LOCUS       DAORHB010000001        23113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_1447_length_23113_cov_4.872365, whole genome shotgun sequence.
ACCESSION   DAORHB010000001 DAORHB010000000
VERSION     DAORHB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 23113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23113
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1447_length_23113_cov_4.872365"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /gene="hutH"
                     /locus_tag="PKM80_00005"
     CDS             complement(<1..1327)
                     /gene="hutH"
                     /locus_tag="PKM80_00005"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNV92317.1"
                     /translation="MQEIIIDGNTLTLEAVEQVALYNAPIRLSESSKEKVIKCRAYVD
                     KIIESGEIVYGLNTGFGKFSTVIIPKENIAELQLNLIRSHSTSVGEPYSEVQTRAIML
                     LRIAVLAKGHSGIRLETLETLVQMLNKGIHPIIPMRGSVGASGDLSPLSHLALVLIGE
                     GEAIYKGERLPGSVAMQKASITPVKLAAKEGLALNNGTQVMAALGVLNLLEAERLCRY
                     ADIIAAISIDALKGTPKAFDELIHNVRPHAGQKTSAKNIRTLLQGSPLRESHLNCGNV
                     QDAYSLRCTPQVNGAVRDACAYVRSVLEVEINSATDNPLIFPDEGKVISGGNFHGEPL
                     AIALDTLAMAVCELASISERRIEQMLNSSLSRGLSPFLATHPGIDSGFMIQQLTAAAL
                     VSENKVLAHPACVDSIPTSANQEDHVSMGSISAIKLCQIVQNVASVLGIE"
     gene            complement(1745..3418)
                     /locus_tag="PKM80_00010"
     CDS             complement(1745..3418)
                     /locus_tag="PKM80_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNV92318.1"
                     /translation="MQKLLQQLNYLNDEHITKVVEVNSHYDPPYMIVEYIHGVSLAKL
                     KTHNPEKLTEDFVRLIAIQIVEAAIKLHQYQLTLSKLALTNVLVKDNNEIVILSSAIT
                     WEERDEREELFSIAGLLAQVLSKSPFYTTIYSQERLANQKFEYIYGVSVPLNKILSEC
                     LHRNIMQRYIGLNEFLDAIINLPPVEKCEIWEEQEKTLPEDMDDISKKEIPKTKIEVT
                     FWVLIILMIGIIVMLLTTNIFSVIFGGKGEKLTYSAWLAPNSESDSVNNPISAKELER
                     NAPLQTSYGELKSGTKSAREDFRKQLPVTNITPSTPSPVVQKPRPPHNMVFIESNSLG
                     LGRLKDNLNHNVSLSSFYMARYEVTQAEWNLYMKPANCSTFGDNLPVDNVSWYDIAIY
                     CNGRSEAEGLTPAYKIRGVGASRVVTCNFKANGYRLPTEAEWELAAKADQLYNYSGSE
                     DPDEISWNRDNSGGKIHTHGTKKPNDYGLYDMTGNVSEWVWDWYDSNYIRSLPTFINP
                     TGPDTGTYKVIRGGSVLNGEGRNLNILWREKGDPNRGYQYVGFRLVRSS"
     gene            complement(3764..6892)
                     /gene="ileS"
                     /locus_tag="PKM80_00015"
     CDS             complement(3764..6892)
                     /gene="ileS"
                     /locus_tag="PKM80_00015"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNV92319.1"
                     /translation="MYKTIDLKESPKHLENRIREYWKNHNLVQKSIDIREGSPRFIFY
                     EGPPTANGKPGIHHVMARTLKDVICRYKTMNGFQVKRKAGWDTHGLPVEIEVEKTLGL
                     QDKRGIEEYGIENFCKKCRESVFSYEKLWREMTELMGYWIDLDHPYITLNNSYIESVW
                     WILNNFFSRNLIYKAYKIVPYCPSCGTPLSSHEVAQGYKDVEDPSIYVKFKALEEDNT
                     YFLAWTTTPWTLISNVALAVHPEETYVKVNYHDQNLILAKSRLQVLDEEAQIISEMKG
                     RELEGLRYQPLFDFVTVDKPAWYVCLGDYVTMSEGTGIVHTAPAFGQDDYSLAMKYDL
                     PFLQPVDGEGKFIEAITPWAHKFVKDADKDIIRHLKESGNLFRREQIKHSYPFCWRCK
                     SPLIYYARESWYIRTTEFKEQLIENNRKIDWYPPFVGEKRFGDWLENNVDWALSRDRF
                     WGTPLNIWICEKCGKQRSIGSIEQLRKEGKLISGEDIPEDLDLHRPYIDEVVLTCSCG
                     NKMYRTPEVIDCWFDSGSMPFAQWHYPFENYDNFEPELFPADIISEGIDQTRGWFYSM
                     LAISTLLKGVSSYKSCLVNDMILDKNGRKMSKSHGNAVDPIELMQNYGADAIRWYLLE
                     VSPPWVPTRFDVDGVKEILGKFIGTLKNVYSFFATYANIDGFIASEYPYNWEREVEID
                     RWIVSRLHSLIVTVRNYFDSYDFTKAVRAIQSFVIDELSNWYVRRSRRRFWASGFDQD
                     KIDAYRTLYQVLVETAKLIAPFAPYLAEELFLNLEAGESIHLELYPVSDNKYIDTQLE
                     KKMQSVIDVVSLGRAARNECQIKVRQPLSEMFVPAKIKEDLLDMLDLVKEEVNIRKIN
                     FVSEQDGFVQYELKPDFKILGPKYGKKVQIINNLLNKTPAAEVLMAFNKTGYFAMDIE
                     QETINLFPEEISVSIIPREGYVFANAKDLFVALDTKLTDELINEGFAREIVNKIQFTR
                     KEMNLDIMDNIEIKIVVTPEIARAIQAHQDYILSETLAKEITILKNPLPDMQGIDING
                     HKIYLSITKLNK"
     gene            complement(6898..7263)
                     /locus_tag="PKM80_00020"
     CDS             complement(6898..7263)
                     /locus_tag="PKM80_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92320.1"
                     /translation="MFILEEKHLEAAKEIAQRNRHKKSCDHCYDRGWIGVSEQNLLVL
                     CPKCVDIDKAMEEWKNYVSENEDLKEHFSELFEEKPIETEQDNLQLQKEREHLKSQPS
                     NPPKFVPGQRRTGHQKKIG"
     gene            complement(7393..7899)
                     /locus_tag="PKM80_00025"
     CDS             complement(7393..7899)
                     /locus_tag="PKM80_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92321.1"
                     /translation="MKIIRHFFCEIKRNREPAPQLCAVCPRIIKCKSFRSWNKLYHQE
                     YLNFVVDITKKFPDKYAMEVYFMAEKQTFVQIVDIATGKIEKVVSLTEIESLSAEDKL
                     TLSRNKTLFIVTHRIEPIVKIELKKSVINAPIQFMDNIPKEEEPIEEVTPLPIKPDKP
                     KAKRPTKS"
     gene            8297..8860
                     /gene="efp"
                     /locus_tag="PKM80_00030"
     CDS             8297..8860
                     /gene="efp"
                     /locus_tag="PKM80_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNV92322.1"
                     /translation="MATMADIRNGMIIEFKDDLYEVVEFLHVKPGKGPAFLRSKLKNI
                     RTGRVLENTFRESDTFNEVRIERTKKEYLYREGDFFVLMDSENYEQMHIDKSLLGEKE
                     KLLTDNMELIVSATPEGEIVGIELPTTVVQTIAECEPNIKGNTASGSGKVAFTETGLR
                     LMVPFFVEIGDKVKIDTRTGEYIERAN"
     gene            8873..9079
                     /locus_tag="PKM80_00035"
     CDS             8873..9079
                     /locus_tag="PKM80_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92323.1"
                     /translation="MGKVKSFAAKVAHDLSTSGKVICPVCNTEVKKIKLITNKKTDGT
                     WAPQKEYVKICKCNEADILAGKNL"
     gene            9266..12400
                     /locus_tag="PKM80_00040"
     CDS             9266..12400
                     /locus_tag="PKM80_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNV92324.1"
                     /translation="MPCLMWKRIVLLLTIVIAGSLSALVPPVPKYKNLPETWEATSLE
                     GNFYTMDNFKGRKPNLPNNILAIRVQFNDVAFRSEAVYPDNIAHDDIFFDRWMLHLKD
                     FFLEASHLQYELDYFLYPQVLTMPHPLSTYGADSTEDIDVNLPQILPDIMDLIDEEVD
                     FTQYGGIIIFHSGTGQESDIESIRPNEIWSTFLTRKMLQKYFEPENDLYPGFITNDGA
                     ILTNVIIVPEDEYQDYFPTEGVDDASSYLFSMYGVLAHQFGHILGLPTLYDNDSSNGI
                     SQGIGNWGLMGTGLWNGNGYVPAQLCAWSRYYLGWDNPVIISQNSLNNAVDHFLNHSP
                     TAIHLYKVPISATEYFLVENRQQNPDGSLDPITNSPSYSFKLLPEGEQDYYPDDPSTP
                     QDESLLPYFNFMENSYLGSEWDFFLPGLGGPIPNNSTYLVDGSGILIWHIDENVINAN
                     FTANFDKNRVNAISAHKGVDLEEADGIQNMDTGLNSIYKWGSPYDSFRDDNNNYFGHQ
                     YHNGVLSLPTSESYYGGIPLEICNISASGLQMTFSVIYSWRLNTSYEGTNPINAAGID
                     FDNDGTDELFYPMPDGRIYIWKNEELMDGYPLYGMPIPYTYTWDGENLFIPMQRDRLC
                     RLYKHNKNESKYVYNSPNYSWVGHPVDLGNSIAMPLKSNNEENTFSLQLYSKEDNSVN
                     TIVDLSGNFKANLVYFHNKLSVVYENTSGEYWLSDIDLADNTITDSKLPISADSTIIA
                     IFKAPVKDDENLIVQCQNSVYCLKGANIVDGFPFVHNLSANSDSSFIAPLSIADVDGN
                     GALDILIGGESDYAVIDYAGRLMNPENPSPQPHSDFISAGILAMDIDNDNKAELIGNF
                     KNNRLKIWENDFRDKRGYPVSFAERSRTLPFVAKASDNNWYVYCATDNGSLFRNQLEG
                     EPKFSAAYNWISEYGNLRRTASIEATDLPNQYQTNELFVPGEVYIYPNPLKSIYRQKI
                     LLNVMTNRDVELELSIFDISGTLVYRQKGFAKAYLKNLDIFDIPSAKLCSGVYIAILR
                     SSEGSKRLKFAIEK"
     gene            12416..13318
                     /locus_tag="PKM80_00045"
     CDS             12416..13318
                     /locus_tag="PKM80_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNV92325.1"
                     /translation="MKQIFTTLCLILISAMCFAGINKDAGTYGYKFLNVPYGPVALSL
                     AGRGVYSSNNSSAFILQPASSCVNDQRLLGITHNMWLADTQANMISYSYSRKIDHFGI
                     AIRNLDYGDIENRDDMGIMIGSYHPIDIDATANYARRLSPNFYTGVNLGVLYQKLNTA
                     SSLALHSDLGFCYLPPVKNTKISLAVRDLGVANKTDDERVKLPTSFEMDINKGFLLAD
                     QQIFLGGSAIQTLDEDLKGSINLEGNLFQIFSIRGGYLLGYDAQDFSAGFGVKYKNIS
                     VDYGYGAFNSELNDVHSFGVTYRF"
     gene            13331..17545
                     /locus_tag="PKM80_00050"
     CDS             13331..17545
                     /locus_tag="PKM80_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNV92326.1"
                     /translation="MNNNLGFMQNRRVGLVFLLICLGLFLQAEITPNQIIFKTTVPVQ
                     IKGTKTGLNAFDSFLTQQGVKQIKPIKGMHQPNYYLANVSEMPSIQTLQQLSFYGIQY
                     VQPNHISKLHTIPNDPLYNQQFHYICSIPDAWNYTTGSKYIKIGVVDSGVLIHHPDLW
                     QNIAINPNEIPDNGIDDDNNGYIDDWCGWDFTDAPEMAENAIGDYIGQDNDVEDENYH
                     GTHVSGIAGAVGNNGIGISGVCWNVGILPIRAGFRTLDNQGYLQDDDVSAAIIYATDN
                     GCNVINMSWGDPDYSAIIADACEYAYSKGVVLIASAGNDGVSGLSYPAKLSTVISVGS
                     VNNAKQRSTFSSYGVDLDLVAVGENVLSTYKLDPGEQYFRLNGTSMSAAFVSGAAALL
                     LSLHPDLSPAEVRASLLTSTDDLYSVGFDIQTGHGLLNVKKILDNLAPPYLEITSPQD
                     QLGISGSVPIIGSVYGPDFADYTVCYKLDDDANDPYWKDVTTHQMDPITHLEQVHNSE
                     LATFYIPDYFPEGKYLLRLQYEKKINNMNRYNYYRTVYVDRSAPVIRSETVSGFTRWN
                     RQNLQYYVSANFNEKVNASLKITASDGQIYNVYSTLADSIHIFKLPSYIPEGMISVQF
                     VATNITGLTSVSDTFNDLLNIHYCLIPHYGYTKEVIGPPQKPLLRKRDYNGNGVSEYV
                     SMEIPASGYGPVKAFELCSGEHIETNDFGDAFWPLDIGSTYTTGSKSELLLLRVENAE
                     LWLTPLGGNYPSNSNDSLVIALDTGISGGVMADYDNDGKTEILLVKNLPAERVIQAYK
                     RMSTGALVQRNTLSNPTTTYSRNNFVPTIIVENLDGDNYPDILCADTDGDIMIYEIIN
                     PTEHHLRWTTRLPVGNTYILTSGDFDGDGNKDFFVAGYNTDITNPNLSFWYGEGFRNT
                     GNNTYSSMGNIMFNEVSTLNSITNADLDKDGCNEIILAITPNLYILKYSNGNFTPVYY
                     DESYRNYQVVTLEDENGAVRIMANIHSDGDNAVSAQWIPSASFTGPATPANLTVKPLN
                     ENSINISWISTGAPEYVLYRKDETGNTVSYNLSAATNYTDTGLQQGKAYQYAIAAIDP
                     SYTPSESNKGPWIEGIPLPKPDVIEISLAGQKELRLLFNQAMPNDIINPGHYFISNGL
                     GNPISANSISGNYGVQLRFRNNFPPIDSLFVIELRNLTGISGVDPVQNFYTFSYQEDV
                     IPPEISQVNVLPSQRGIIITFSEEILPNPALHLANYILHCPQNDPDNTIVSIAHSVDT
                     VTITFAEKLKFTNYAYYLEINNITDLAGNLISPQGNLARFGIQNLNNLDHLNVFPNPV
                     TSKHPQEATFINFPLNKKGKIAIYNSGGQLVFKDDIGPFFEGSNNITYTWNLKNNDQR
                     PVSSGVYFYVVEMGGKLKRGKLAIIK"
     gene            17713..18015
                     /locus_tag="PKM80_00055"
     CDS             17713..18015
                     /locus_tag="PKM80_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HNV92327.1"
                     /translation="MISTIFLLLNILDGHSTYLVLKPNYYHREKNPVARWVFKKLSIP
                     NGIIIFKTVLMAILILAISYYTAWDSLTINIAMTVADLLFLIVVIHNYRLYRRIKH"
     gene            complement(18077..19429)
                     /locus_tag="PKM80_00060"
     CDS             complement(18077..19429)
                     /locus_tag="PKM80_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNV92328.1"
                     /translation="MIKAEVFRQYDIRGIVNEELDENSFYLIGKGFGTYIRNKNLKTI
                     VLGGDARHSTPSFMQAFSKGALETGCDIIDLGIVPTPVLYFSIWKLKTDGGAMVTASH
                     NPPRYNGCKLNLGLSSVYADELQKVLQIIQKGDFVSGQGNYVKNNDMGDIYIDYVVNG
                     IKLQRPVKVIVDGGNGVGGPYLTAILRRLGCEVIDMFCEPDGDFPNHHPDPTIAKNME
                     ALSKAVIENKYELGIGLDGDADRIGVVDEQGKLLLGDQILNILARDYLKHNPGKKIIA
                     DVKCSMNLFNDIIKYGGEPIMYKTGHANIKMYMQEKKVEFAGEMSGHIFLADRYLGFD
                     DAIYVSCRFCEIVAQTLNPVSSFLADQPKLFNTPELHTSCPDAKKFEVVAKVCEEFKK
                     EGYEVNDIDGARITFSDGWGLIRASNTTPIIVTRYEAITEERMNEIKGLIEGKIQKYL
                     "
     gene            complement(19446..20036)
                     /gene="rdgB"
                     /locus_tag="PKM80_00065"
     CDS             complement(19446..20036)
                     /gene="rdgB"
                     /locus_tag="PKM80_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNV92329.1"
                     /translation="MISDILVATHNPDKLKELQELLNNLKVQLHFLDELPQFLPSEEN
                     GKTIYENAMQKALEAAKYSGLLTLADDTGLFIEELNGAPGILSARFAGNSCSYSDNRK
                     KVLNLLQGVENRKAYFETVVALALPDGIVAVLGGKLEGEITTEELGDNGFGYDSIFAV
                     GDKTYAEMDSEQKNKISHRALAMKAIIPILEKVIID"
     gene            complement(20033..20563)
                     /locus_tag="PKM80_00070"
     CDS             complement(20033..20563)
                     /locus_tag="PKM80_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNV92330.1"
                     /translation="MKEDDVIIGKEIPVEEQEKIKDKIIDDDVDSTKLQFYEDLRKKV
                     RSWTNTQLGPLGGKLGEYVFLLPDFFILVCRIAVDKRVSVKQKLLMGGVIAYVMMPID
                     LIPDFIPVLGFVDDLVLIVLGLNMILNELNPQIVLDNWSGEGEVLEHLKKITALAEQF
                     LDKHLLNKIKNVLKKI"
     gene            complement(20774..>23113)
                     /gene="ppdK"
                     /locus_tag="PKM80_00075"
     CDS             complement(20774..>23113)
                     /gene="ppdK"
                     /locus_tag="PKM80_00075"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNV92331.1"
                     /translation="KTNNERFAWDSYRRFVQMYSDVVLGMKPANKEEHDPFEVIINQV
                     KKEKGISSDLELTTEDLKQLVTLFKQEVQEKIHKKFPDDPWEQLWGAIFAVFDSWNND
                     RAIFYRKLNNIPDEWGTAVTVQAMVFGNMGYTSATGVAFTRDAATGENLFNGEYLINA
                     QGEDVVAGIRTPQQIAKIGSQRWAKLAGISEEERKEKYPSLEETMPETYKELYEIQKK
                     LENHYREMQDLEFTIQEGKLWLLQTRTGKRTGAAMVKIAMDMLREGIIDEKTALNRIE
                     PAKLDELLHPVFTKEGLAKAKVITLGLPASPGAATGQIVFFADDAEAWAENNKKVILV
                     REETSPEDLRGMSVAQGILTARGGMTSHAAVVARGMGKCCVAGAGKLHIDYKNRTMTV
                     NGKTYKEGDWISLNGSTGQVLEGKIDTQDPELSGDFGAIMQLADKYTRMKVRTNADTP
                     KDASVARHFGAQGIGLCRTEHMFFEGERINSMREMILAEDEQGRREALKKLLPMQRSD
                     FEGIFTAMNGFPVTIRLLDPPLHEFVPQEEEAQQEIAKELGIPLEIVKARVASLHEVN
                     PMLGHRGCRLGNTYPEITEMQSRAIMEAALAVKARGINVLPEIMVPLTSIPAEFEMQE
                     TIIRETAEKVFTDMNDRVDYLVGTMIEIPRATLLADKIAEHAEFFSFGTNDLTQMTFG
                     FSRDDAGVFLPIYIEKNLLKQDPFQVLDREGVGQLVKIGVERGRKTRPELKIGICGEH
                     GGEPSSVEFCHMVGMNYVSCSPYRVPIARVAAAKAAISE"
BASE COUNT         7069 a         4413 c         4042 g         7589 t
ORIGIN      
        1 gttcaattcc caaaacagaa gccacatttt gcactatctg gcaaagtttt atagctgaaa
       61 ttgaacccat agacacatga tcttcttgat tggcagatgt aggaatagaa tcaacacagg
      121 caggatgggc tagaacttta ttttccgaaa ccaacgctgc agccgtcaat tgctgaatca
      181 taaaaccaga atcaattcca ggatgcgttg ctaaaaatgg tgaaagccct ctggatagag
      241 aagaatttaa catctgttca attctgcgtt cagatatgga agcaagctca caaaccgcca
      301 tagctaaagt atctaaagca atagctaagg gctcaccatg aaaatttccc cctgaaataa
      361 ctttcccctc atcaggaaaa atcaacggat tatccgttgc cgaatttatt tccacttcca
      421 aaacactacg cacataagca caggcatctc taacggcacc attaacttgt ggagtgcatc
      481 gtaagctata agcatcttgc acattcccac aatttaaatg ggattcccgc aaagggctac
      541 cctgtaaaag agttctgata ttttttgcgg atgttttctg accggcatga ggccttacat
      601 tatgaatcag ttcatcaaat gctttgggtg ttccttttaa ggcatctatg ctaattgcag
      661 caataatatc agcataacga cataaacgtt ctgcttccag aagatttaga actcccaaag
      721 cagccataac ttgagtccca ttatttaagg ccaaaccttc tttggcagca agttttacag
      781 gtgttatact tgctttttgc attgccacac ttcctggcaa acgctcacct ttataaattg
      841 cttccccttc accaatcagc actaaagcca aatgtgaaag aggagaaaga tctccactgg
      901 ctccaactga accccgcatc ggaattatag gatgaattcc tttattcaac atctgtacca
      961 aggtttccaa agtctctaat cgaatgccag aatgaccttt ggctaagaca gcaatcctta
     1021 aaagcattat tgcccgcgtt tgcacttcag aatatggttc tcccacgctg gttgaatgac
     1081 tgcgaattag atttaattgc aattcagcta tgttttcctt aggaataatt acggtgctga
     1141 atttaccaaa accagtattc aaaccgtaga caatttcacc actttctatt attttatcca
     1201 cataagcacg acatttaatc actttttcct tgctggattc cgacaggcga atcggagcat
     1261 tataaagagc aacctgctcc actgcttcca gcgttaaagt attgccgtca ataataattt
     1321 cctgcatttt atcctctata tatagtaatt cttatgatgg taaacctaaa aaatgataat
     1381 tttactcctt tatttctata gatatgattt tgtcaagcca agaaagctct cacattggtt
     1441 atattgtatt ttatcctttc tgccttgctt gattctctaa cctcaatcct cattatctcc
     1501 ttctccactc atagttcagg atgggtttag gaggagagaa tgaggagttg atgaggagtg
     1561 gttaacaagc aagatgtaag taaccacttg agaaataaag tggtagcaag aaaaaacaat
     1621 tacacttaat tgaagggatt cttgctttcg cagaattaca gaaacgattg cagaaagatt
     1681 tttaccaaat tggaaatcgg taaactatct tcaaattccc tcttatcata ttataaggta
     1741 acaatcagct gctgcggact aacctaaagc caacatactg ataaccgcga ttcggatctc
     1801 ctttttctct ccagaggatg tttaaatttc tgccttctcc atttaaaaca cttcccccgc
     1861 ggataacttt ataggttccg gtatctggtc ctgtaggatt tataaaagta ggcaaagaac
     1921 gaatataatt ggaatcatac caatcccaaa cccattcgga aacatttccg gtcatatcat
     1981 ataagccata atcattgggt tttttggtcc catgagtatg aattttgcca ccgctattat
     2041 ctctattcca agatatctca tcaggatctt ctgaaccact gtaattataa agttgatctg
     2101 ctttggctgc taattcccat tccgcttcgg ttggtagacg gtatccatta gctttaaaat
     2161 tgcatgtcac aactctggaa gcacctactc ctcttatttt gtaagccggg gttaaacctt
     2221 cagcttcact tctaccatta caataaatag ctatatcata ccaacttaca ttatccacag
     2281 gtaaattatc tccaaaagtt gaacaatttg ccggtttcat atagaggttc cattctgctt
     2341 gggttacttc atatctggcc atataaaaag aagatagaga aacattatga ttcaggttat
     2401 ctttcaatct tcccaaacct aaggaattgg attcaataaa taccatatta tgtggaggtc
     2461 ttggtttttg aactacgggt gaaggagtag aaggagtgat attggtaaca ggaagctgct
     2521 ttctaaaatc ttctcgagca gattttgttc ctgattttaa ttcaccataa gatgtttgca
     2581 aaggtgcatt gcgttctaat tcttttgctg atataggatt atttacacta tccgattcgg
     2641 aatttggggc tagccaggca gaataagtta atttttctcc cttacctcca aaaataacgc
     2701 tgaaaatatt ggtggtaagc aacataacaa ttataccaat cattagaata attaaaaccc
     2761 agaaagtaac ctcaattttg gtttttggga tttctttttt gctgatgtcg tccatatcct
     2821 cagggagtgt tttttcctgt tcttcccaga tttcgcattt ttctactgga ggtaaattga
     2881 taatggcatc gagaaattca ttcaatccaa tatatcgttg cataatattg cgatgcaaac
     2941 attcagataa aattttatta aggggaacac tcaccccgta tatgtactca aatttctgat
     3001 tagctaaccg ttcttgagag tatattgtgg tataaaacgg acttttagaa agtacttgag
     3061 ctaacaaacc agctatagaa aacagttctt ctctttcgtc tctttcttcc caagtgattg
     3121 cagaagaaag aattactatc tcattattat cttttaccag aacattagtt aaggctaatt
     3181 tacttaaagt taactgatat tgatgaagtt ttatggcagc ttcaactatc tgaatagcta
     3241 taagacgaac aaaatcctca gtcaattttt ccggattatg cgtttttagc ttcgctaagg
     3301 agaccccgtg aatatattca acaatcatat agggagggtc gtaatgagaa tttacttcta
     3361 ctactttggt aatgtgttca tcattcaagt aatttagctg ttgtaaaagt ttctgcagag
     3421 aaaatatttt ggttacattg cttaaacttg ctttgtagaa gagctttagt aaatattctt
     3481 taccatcctt ttcagctatg tattttatcc cttctgcatc tttatttaat aaacggacta
     3541 ttttatagtc ccgaaaactg ttgccaggat ttaagagaag ttgagaacgc tttatatcac
     3601 ctttatttac ttgctgtttc gaatcttttt tatctggttc cttttgctga gttttttccg
     3661 cttcttgaat ttgattttct ccacagacag gacaaaactt cgcttgcgct ggtatagagt
     3721 ttccacaatt aatacagaat ttattgatga ctgcctccta accctattta tttaatttag
     3781 taatgcttaa atatatctta tgcccattta tatctatgcc ttgcatatcc ggtagagggt
     3841 tctttaatat agttatttct ttagccaggg tttcgcttaa tatataatct tgatgggctt
     3901 gaattgccct agcgatttct ggagtaacta caattttgat ctcaatatta tccataatat
     3961 ccagattcat ttctttgcga gtaaactgaa ttttatttac tatttctcga gcaaaacctt
     4021 cgtttataag ttcatcagtt agcttagtat ctaacgctac aaaaagatct ttggcattgg
     4081 caaaaacata tccttcacgg gggataatgc taacagatat ttcttctgga aataaattaa
     4141 tcgtttcttg ctcaatatcc atagcaaaat aaccggtttt gttaaaggcc attaaaactt
     4201 ctgctgcagg tgttttattt agaagattat taatgatctg cactttcttg ccgtatttag
     4261 gacccaaaat tttgaaatcg ggcttcaatt catactgcac aaagccatcc tgttcgctta
     4321 caaaattgat tttacggata ttaacttcct cttttacgag atccaacata tccagtaaat
     4381 cttcttttat cttagctgga acaaacattt cacttaaagg ttggcgaact ttaatctggc
     4441 attcatttct ggctgctcta cctaaagaaa caacatcaat cacactctgc attttctttt
     4501 ccagttgtgt gtctatatac ttattatccg aaacaggata caattccaga tgaatactct
     4561 cgcctgcttc cagattcaaa aatagttctt ctgccagata aggagcaaag ggggcaatta
     4621 gtttagctgt ttctacaaga acttgatata aagttcgata ggcatctatc ttgtcctggt
     4681 caaatcctga agcccaaaaa cggcgtctac ttcttcgaac ataccaattg gaaagttcgt
     4741 caataacaaa actctgaata gctctcacgg ctttggtaaa atcgtaacta tcaaaatagt
     4801 tgcgaacagt tactataaga gaatgtaagc gagaaactat ccatctatct atttctactt
     4861 cgcgttccca attataagga tattcagaag caatgaaacc atcaatattg gcataagtag
     4921 caaagaaaga atatacattt ttaagcgttc caataaactt tcccaaaatt tctttaacgc
     4981 catctacatc aaaacgcgta gggacccaag gcggagaaac ttctaaaaga taccaacgaa
     5041 ttgcatctgc cccataattt tgcataagtt caattgggtc aacggcattt ccatgagatt
     5101 tactcatttt cctgccattt ttatccaaaa tcatatcatt tactaaacag cttttataac
     5161 tagaaacacc ttttaacaag gtagaaatgg ccagcataga ataaaaccag ccacgagttt
     5221 gatctatccc ttcagaaata atatctgcag ggaatagttc cggctcgaaa ttatcataat
     5281 tttcgaaagg ataatgccat tgagcaaacg gcatagaacc actatcaaac caacaatcaa
     5341 taacttctgg agtgcgatac attttatttc cacaggaaca agtaagaaca acttcatcaa
     5401 tataggggcg atgtaaatcc aaatcttcag gaatatcctc tccggagatt aatttcccct
     5461 cttttcgtaa ctgctcaata gatccaatgg aacgctgttt tccacatttt tcacaaatcc
     5521 agatatttag tggcgtcccc caaaaacgat ctctggaaag agcccagtcc acattatttt
     5581 ccaaccagtc gccaaaacgc ttttctccta caaaaggagg ataccagtca attttacggt
     5641 tgttttcaat tagctgttct ttgaattctg tggttctgat ataccagctt tcgcgtgcat
     5701 aataaattaa agggcttttg catctccagc aaaaaggata gctgtgtttt atctgctctc
     5761 tacggaataa attgccactt tcttttaagt gacggataat atctttatcg gcatctttaa
     5821 cgaatttatg agcccaaggt gtaatggctt ctatgaattt accttcgcca tctactggct
     5881 gaagaaaagg tagatcatat ttcatagcta aagaataatc atcctgacca aaagctggag
     5941 ctgtatgaac aattccggtt ccttcactca ttgttacata atcccctaaa cagacatacc
     6001 aagcaggttt atcaactgtt acaaaatcga aaagaggttg atatcgaaga ccttccagtt
     6061 cgcggccttt catttctgaa attatttgag cttcttcatc cagaacttga agacgcgatt
     6121 tggccaaaat taaattctga tcgtgataat taactttcac ataggtctct tccggatgaa
     6181 ccgctaaagc tacattggag ataagagtcc agggagtagt tgtccaagcc aagaaatatg
     6241 tattatcttc ttccaatgct ttaaacttta catatataga aggatcttcc acatctttat
     6301 aaccctgagc tacttcatga gaagataaag gagttccaca acttgggcaa tagggaacta
     6361 ttttataggc tttataaata agattgcgag agaaaaagtt atttaaaatc caccagacgg
     6421 attctatata actattattt agagtaatat atggatgatc aaggtctatc caataaccca
     6481 ttaattctgt catttcccgc caaagctttt catagctgaa aactgattcg cggcattttt
     6541 tacagaaatt ttcaattcca tattcttcga tgcccctttt atcttgaaga ccaagggttt
     6601 tttcaacttc aatttcaaca ggcagaccat gggtatccca tcctgctttt cttttaactt
     6661 gaaagccgtt cattgtttta taacggcaga taacatcttt caaagttctg gccattacat
     6721 gatgaattcc aggtttacca tttgcggtag ggggtccctc gtaaaagata aaacgagggc
     6781 tgccttcgcg gatatctatg cttttttgta ctaaattatg gtttttccag tattcgcgaa
     6841 tacggttctc taaatgctta gggctttctt ttagatcgat agttttatac atatacctta
     6901 tccgattttc ttctgatggc ctgtccttct ttgcccggga acaaatttag gaggattaga
     6961 aggttgactt ttaagatgtt cacgttcctt ttgcaactga agattatctt gttctgtttc
     7021 tatcggtttt tcttcaaaga gttcactgaa atgctctttc aagtcttcat tttcggaaac
     7081 ataattcttc cattcttcca tagctttatc tatatctaca catttgggac agaggactag
     7141 taaattttgt tcgctaactc caatccaacc acgatcgtag caatggtcac aacttttctt
     7201 gtgacgattt ctttgggcaa tttcttttgc cgcttctaag tgtttttctt cgagtataaa
     7261 cataatttct ccttacttgg aacatagagg tataaaaaca cctcaataaa tttcagcatt
     7321 gtgagccatt tattttaagc gcacaattct gtaaaaggaa atttttgttc ctaaaattac
     7381 tattctgctt ttttagcttt tggtaggtcg ttttgccttt ggtttatctg gcttaattgg
     7441 caaaggcgtt acttcctcaa ttggttcttc ctccttagga atattatcca taaactgaat
     7501 aggagcatta atcactgatt tcttaagctc gatttttaca atcggctcaa ttcggtgagt
     7561 tacaataaac agggtcttat tgcgagataa agtaagttta tcttctgcag ataaagactc
     7621 aatttcggtt aagctaacta ccttttctat tttaccggta gctatatcta cgatttggac
     7681 gaaggtctgt ttttcggcca taaagtaaac ctccattgca tatttatcag gaaatttctt
     7741 ggtgatatcc actacgaaat taagatattc ttgatgatat aatttattcc aggaacgaaa
     7801 actcttgcac tttataatac gaggacacac tgcacacaat tgaggtgccg gttcgcgatt
     7861 gcgttttatt tcacagaaaa agtgcctaat tattttcata tcttttcctt atcttgtaat
     7921 tcctttatac tgtcaagaaa atttatgttt tccaacaatc cctccaataa tatctacaat
     7981 cttgttcatc ttatacctat tatctttttg tcgaggggct tacgccacca tcgctatcat
     8041 tgaatttccc gttgggttat taaggagaaa tatagtttta taaaatcctc ttaatcctga
     8101 aatcctaaaa tcttttttgc tagaattcaa attctaaaat cagcccccta tttgagaact
     8161 tgatagaaga ttacctcatc agaatgctca gtgtttataa aatagaaggg gatctgcact
     8221 tattttaaaa attcccttgc caaaaaccct ttatctaaaa ttatgactta cttagtctaa
     8281 atataaggaa acaaatatgg ctacaatggc tgatatcaga aatgggatga tcattgaatt
     8341 caaagatgat ctttatgaag ttgtggaatt tcttcatgta aaaccaggta agggacctgc
     8401 tttcttgcgt tccaagctga aaaacatacg cacgggaaga gttctggaaa atacttttcg
     8461 agaaagtgat acttttaatg aagttcgtat tgaacgcaca aagaaagaat atctttatcg
     8521 ggagggagat ttttttgtgt tgatggatag tgaaaactac gaacagatgc atattgataa
     8581 atctctttta ggcgaaaaag aaaagttact taccgataat atggaattaa tcgtttctgc
     8641 tactccggaa ggagaaatag taggaataga attgccaaca acagttgttc aaactattgc
     8701 tgaatgcgaa cctaatataa aaggtaacac tgcttctgga agtggcaaag tagcatttac
     8761 agaaacaggt ttaagactga tggttccctt ctttgtagaa ataggggata aagtaaaaat
     8821 agacaccaga accggtgaat atatagagag agctaattaa aaagaggata aaatgggaaa
     8881 agtaaaaagt tttgccgcaa aagttgcaca tgacctttcc acttcaggga aagtgatttg
     8941 cccggtatgc aatacagaag tgaagaagat taaacttatc acgaataaga agacagatgg
     9001 aacctgggca cctcagaagg aatatgttaa gatttgcaaa tgcaacgaag cggatatcct
     9061 tgcgggtaaa aatctgtaag gacaaccaat ctgcattagt aaaggcgaat taattacgcc
     9121 tgaggagaaa tgcgtctctt caggcgtttt ttcattgacg gtttgtataa gctttataaa
     9181 aaggttttca attatagcta tctgctttca gtagtttggt agcggatttt ttgtcttcat
     9241 aaatttattt tcctggagga ttgaaatgcc ttgccttatg tggaagcgga tagttctact
     9301 gctaacaatt gtaatagctg gttctttatc agctctggtt cctcctgttc ctaaatataa
     9361 gaatctacct gaaacttggg aagctacttc tttggaaggt aatttttata caatggataa
     9421 ttttaaaggc aggaagccaa atttacctaa taatattttg gctataagag tccaattcaa
     9481 tgatgtagcc tttcgttctg aagcagtata tcctgataat atagcccacg atgatatctt
     9541 ttttgatcgc tggatgctcc atttgaagga tttttttctg gaagcgagtc atttacagta
     9601 tgaacttgat tactttcttt atccgcaagt gcttacaatg cctcatcctt tatctactta
     9661 cggtgccgac agcacagaag atatagatgt aaaccttcct cagattttac ctgatattat
     9721 ggatttaata gatgaagaag tagattttac tcaatatggc ggaattatta tttttcattc
     9781 tggaacgggt caggaatcgg atatagagtc cattcgtcct aacgagatat ggagcacttt
     9841 cttaacccgt aaaatgctgc agaaatattt tgagccggag aatgacttat atcctggttt
     9901 tattaccaat gatggagcta ttttaacaaa tgtaattatt gtccccgaag atgaatatca
     9961 ggattatttc ccaactgaag gtgtagatga tgcttcgtcc tatcttttta gtatgtatgg
    10021 agtattagct caccaatttg ggcatatttt aggtttgcct actttatatg ataatgacag
    10081 cagtaatgga atcagccagg gaattggtaa ttggggttta atgggcacgg gtttatggaa
    10141 tggtaatggc tatgttccag ctcagctttg tgcttggtct cgttattatt taggttggga
    10201 caatccagtt atcatatctc aaaatagttt aaataatgca gttgatcatt ttctaaatca
    10261 ttcgcctaca gctatacatc tttataaagt gccaatatcg gcaacggaat attttttggt
    10321 tgaaaacaga caacaaaatc ccgatggaag tttagaccct ataactaata gccccagtta
    10381 tagttttaag cttcttccgg agggtgagca ggattactat cctgatgatc cttctacacc
    10441 acaggatgaa tctctgctac catattttaa ttttatggaa aacagctatt taggcagtga
    10501 atgggatttt ttcctacctg gtttgggagg tccgattccc aataatagta catatcttgt
    10561 agatggctcc ggaattctta tctggcacat agacgagaat gttatcaatg caaattttac
    10621 tgcgaatttt gataaaaacc gggtaaatgc catttctgct cataaaggag tagatttgga
    10681 agaagcggat ggaattcaaa atatggatac cggtttgaat agtatttaca aatggggaag
    10741 tccttacgat agctttcgag atgataacaa caattatttt ggtcaccaat atcataatgg
    10801 agtgctttca ttgccaactt cagaaagcta ttatgggggt attcctttag aaatatgcaa
    10861 tatttcggcc agtggattac agatgacttt ttccgttata tacagctggc gtttaaatac
    10921 ttcctatgaa ggaactaatc ctattaatgc agctgggata gattttgata atgatggaac
    10981 tgatgaactt ttctatccta tgcctgatgg taggatttat atttggaaaa atgaagagct
    11041 gatggatggc tatcctttat atggaatgcc tattccctat acttatactt gggatggaga
    11101 aaatcttttt attcctatgc aacgggatcg gctttgccgt ttatataaac acaataaaaa
    11161 tgaaagcaaa tatgtttata actctcctaa ttacagttgg gttgggcatc ctgtagattt
    11221 aggaaattct atcgctatgc ctttaaaatc caataatgaa gagaatacct tttctcttca
    11281 gctttacagt aaagaggata atagcgtaaa tacaattgtg gatttgtctg gtaactttaa
    11341 agccaacctg gtatattttc ataacaaact ttccgttgtt tatgaaaaca catcgggaga
    11401 atattggcta agtgatattg acttagcgga taatacaata actgattcta agcttcctat
    11461 ttctgccgat tcaacaatca tagcgatctt taaagcgcca gttaaagatg atgaaaactt
    11521 aattgtacag tgccaaaaca gtgtttattg tttaaaggga gcaaatatag ttgatggctt
    11581 tccctttgta cataatctat ctgccaattc agacagtagt tttattgctc ctttaagcat
    11641 agccgatgtt gatggaaatg gtgctttgga tattttgatt ggaggggaaa gtgattatgc
    11701 tgttattgat tatgctggca gattaatgaa tccagaaaat ccaagtcctc agcctcattc
    11761 agattttatt tctgctggaa tattggcaat ggatatagac aacgataata aagccgagtt
    11821 gataggcaat tttaaaaata accgccttaa gatatgggaa aatgattttc gcgataaaag
    11881 aggttatcct gtttcttttg cagagcgcag tagaacctta ccttttgttg ctaaggcatc
    11941 agataacaat tggtatgttt attgtgccac ggataatggt tctctgttta gaaatcaatt
    12001 ggaaggagaa cctaaattca gtgcagctta taattggatt agtgaatatg ggaatctaag
    12061 aagaacggct tcaattgaag ctactgattt acctaatcaa tatcagacca atgaactgtt
    12121 tgtgcctggt gaggtctata tttatcccaa tcccttaaag tccatatatc ggcaaaaaat
    12181 attattgaat gtaatgacca atcgcgatgt cgaactggaa ctaagtatat ttgatatcag
    12241 cggtactttg gtataccgtc agaaaggttt tgccaaagca tatcttaaaa atcttgatat
    12301 ttttgatatt ccttctgcca aactatgcag tggggtttat atagcaatat taagatcctc
    12361 agagggcagc aaacgcctca agtttgctat agaaaaataa cgggagcgta atctaatgaa
    12421 acagatattt actaccttat gcctaatcct gatttcagca atgtgttttg ctggaattaa
    12481 taaagatgcc ggaacttacg gctataaatt tttaaatgtt ccttatggac cggtggcttt
    12541 atctctggca ggaagaggag tgtatagttc taataattca tcagctttta ttttacaacc
    12601 tgcttcttcc tgtgttaatg atcaacgcct tttgggaata actcataata tgtggttggc
    12661 agatacccag gcaaatatga tatcctattc ttattcccga aaaatagatc attttggcat
    12721 tgctatccgc aatttggatt acggtgatat agaaaacaga gatgatatgg gtattatgat
    12781 tggtagctat catccaatag atattgatgc tacggcaaat tatgccagac ggctaagtcc
    12841 taacttttat acgggtgtaa atttaggagt tttgtatcaa aaattgaata cagcttccag
    12901 cctagcttta cattcagacc ttggtttttg ctatcttcct cctgttaaaa acaccaaaat
    12961 atctttagca gttcgggatt taggggtggc aaataaaact gatgatgaaa gagtaaaact
    13021 acctactagt tttgagatgg atatcaacaa gggttttctc ttagccgatc agcaaatttt
    13081 tttggggggc tcagctattc agaccttgga tgaagacctc aaaggtagta tcaatctgga
    13141 aggtaatctc tttcaaatat tttctataag gggaggctat ctgttgggtt atgatgctca
    13201 ggattttagc gctggttttg gagtgaaata taaaaacatt tctgtagatt atggatatgg
    13261 agcttttaac agtgaattga acgatgttca ttcctttgga gtaacttacc gattttaaca
    13321 gaggtataag gtgaataata atcttggttt tatgcagaac cgcagagtcg gattagtatt
    13381 tttgttaata tgtttggggc tctttctcca agcagaaatt actcctaatc agattatatt
    13441 taaaacaact gttccagtac agattaaagg aacaaaaaca ggcttaaatg cctttgatag
    13501 tttccttact cagcaaggag ttaagcaaat aaagccaatt aagggtatgc accaacccaa
    13561 ttactatctg gctaatgttt ctgaaatgcc aagcattcaa actttgcaac agcttagctt
    13621 ttatggcatt caatatgttc agccaaatca cataagtaaa ttacatacta ttcccaatga
    13681 tcctttatac aatcaacaat ttcattatat atgcagcatt ccagatgcct ggaattatac
    13741 aaccggcagc aaatatatca aaatcggtgt tgtggattct ggagtattaa ttcaccaccc
    13801 agatttatgg caaaatatag ctataaatcc aaacgaaata cccgataatg gaatcgatga
    13861 tgataataat ggctatatcg atgactggtg tggatgggat tttactgatg cccctgagat
    13921 ggctgaaaat gctattggag attatatcgg acaggataat gatgttgaag atgaaaatta
    13981 ccatgggacg catgtatcag gtattgctgg agcagttgga aataatggaa tagggattag
    14041 tggagtttgc tggaatgtag gtattttacc aatcagggca ggtttcagaa ctttagataa
    14101 tcaaggttat ttacaggatg atgatgtttc tgcagcgata atttacgcta ccgataatgg
    14161 ttgtaatgtt attaatatga gttggggcga tcctgattat tcagcaataa ttgctgatgc
    14221 ttgtgaatat gcatatagca agggtgttgt tttaattgcc tctgcgggaa atgatggagt
    14281 ttcaggttta agttatcctg cgaaactttc tacagttatt tcggttggtt ctgtgaataa
    14341 tgctaaacag cgttccacct tttccagtta cggagtggat ttagatcttg ttgctgtagg
    14401 cgaaaatgtt ctttctactt ataaacttga tccaggagaa caatattttc ggttgaatgg
    14461 cacttctatg agtgcagctt ttgtctcggg agcagcggcc ttacttttat ctttgcatcc
    14521 agatttgagt cctgcagaag taagagcttc gttattaact tctacggacg atttatattc
    14581 tgttggtttt gatattcaaa ctgggcatgg tttgctaaat gttaaaaaaa tactggataa
    14641 tcttgctccg ccctatctgg aaattactag tcctcaagat caattaggga taagtggttc
    14701 agtaccaatt atcggttctg tttatggacc agattttgcc gattatacag tatgctataa
    14761 attggatgat gatgccaatg atccttactg gaaggatgtt actactcacc aaatggatcc
    14821 aattactcac ttggaacaag ttcataattc tgaattagct actttttata ttccggatta
    14881 tttcccggaa gggaaatatc tgctccggtt gcaatatgaa aaaaaaataa ataatatgaa
    14941 tcgctataac tattacagga ctgtttatgt agatcgtagt gcaccagtta taaggtcgga
    15001 aacggtttct ggatttactc gttggaaccg ccaaaatcta caatattatg tttcagcaaa
    15061 tttcaatgag aaggtaaatg ccagtctgaa aataactgct tccgatggcc agatatacaa
    15121 tgtttattct actcttgcag atagcattca tatttttaag ttaccttcct atattcctga
    15181 agggatgatc agcgttcagt ttgttgctac taatataacg ggtttaactt ctgtctctga
    15241 tactttcaat gatttgttga acattcacta ttgtttaatt ccacattatg gttatactaa
    15301 agaagtaata ggtcctcccc aaaaaccttt actccgtaaa agagattata atggcaatgg
    15361 cgtttctgaa tatgtttcta tggaaattcc agcgagtgga tatggtcccg taaaagcatt
    15421 tgaattgtgt tctggagagc acatcgaaac taatgatttt ggggatgctt tttggccttt
    15481 ggatattggt tccacctata caacaggttc caagagtgaa ttgttattgt taagagtaga
    15541 aaatgctgaa ttgtggctta ctcctttagg aggaaattat ccttcgaata gtaacgatag
    15601 cttagtaatt gcgttggaca ctggaatttc cggaggagta atggccgact atgataatga
    15661 tgggaaaacc gagattttgt tggttaaaaa tctacctgcc gaacgcgtta ttcaagcata
    15721 caaaagaatg tccacaggtg ccctggttca aagaaacacc ctcagcaatc ctactacgac
    15781 ttatagtcgg aataattttg tgcctaccat cattgtagag aatctggatg gcgataacta
    15841 tcccgatatc ttatgtgccg atacagatgg cgacataatg atttatgaaa ttattaatcc
    15901 tacagaacac catttacgat ggacaactcg gttgcccgta ggcaatactt atattcttac
    15961 atcaggggat tttgatggtg atggcaacaa ggatttcttt gtggcaggat ataatacaga
    16021 cataactaat ccaaatctta gtttctggta tggcgaaggt tttcgtaata ccggaaataa
    16081 cacatatagc tctatgggca atattatgtt caatgaggtc tcaactttaa attctattac
    16141 taatgcagac ctggataaag acggttgtaa cgagattatt ttagcgatta ctcccaattt
    16201 atatattctt aaatatagca acggtaattt tacccctgta tattatgatg aaagctatag
    16261 aaattaccaa gttgtcactt tggaagatga aaatggtgct gtgcggatta tggccaatat
    16321 acatagcgat ggagataatg ctgtttctgc acagtggata ccttcagcat catttacagg
    16381 acccgcaact ccagctaatt taacagtgaa acctttaaat gaaaattcta taaatatttc
    16441 ttggatatct acaggtgcac cggaatatgt tttatatcgt aaagatgaaa ccggaaacac
    16501 agtttcgtat aatttaagtg cagcaaccaa ttataccgat actggtttac aacaggggaa
    16561 agcatatcaa tatgcaattg cagctataga tccatcttac actcccagtg aaagtaataa
    16621 aggcccttgg atagaaggta ttcctttacc caaaccggat gttatagaaa taagtcttgc
    16681 aggacaaaaa gagctgcgtt tgttatttaa tcaggcaatg cccaatgata ttattaatcc
    16741 aggccattat tttatcagta acgggctggg gaacccaatt tctgctaata gtatttccgg
    16801 aaattatgga gtccagctaa gatttcggaa taattttcca cctattgatt ctctgtttgt
    16861 aattgagcta agaaatttaa ctggaatcag tggtgttgat cctgtacaaa acttttatac
    16921 tttttcttat caggaagatg taattcctcc agaaatatcc caagtgaatg ttctgccttc
    16981 ccaacgcgga ataataataa cattcagcga agaaatattg cccaatccag ctttgcatct
    17041 tgccaattat atattacact gtccacaaaa tgatcccgat aatacaattg tttccatagc
    17101 tcattctgta gatacggtta caatcacttt tgccgaaaag ctgaaattca ctaattatgc
    17161 ttactacctg gaaattaata atataaccga tttagcgggt aacttaattt ccccgcaggg
    17221 caatttagcc cgcttcggaa ttcaaaacct caataatctc gatcacttaa atgttttccc
    17281 caatcctgta acttccaaac atcctcagga agctactttt attaattttc ctttaaacaa
    17341 aaaagggaaa attgctattt acaacagtgg gggacagcta gtttttaaag atgatatcgg
    17401 tccttttttt gaaggtagta ataatattac atatacttgg aatctaaaga ataatgatca
    17461 gcgacctgtt tccagtggtg tctattttta tgttgtagag atgggtggaa aattaaaacg
    17521 cggcaaactt gccataatta aataagctat gtccttgcta aaaaacacac ctggatttac
    17581 accttattta tatgtaaacc gtaaaatgag ccgcactcta aataaatata acctgataaa
    17641 aagattttct gctaccttga tatgttttgg aagataataa tatctgtgtt taattctcct
    17701 gtatacactc ttgtgattag cactatattt ttattgctta atatcctgga tggacattca
    17761 acttatctgg ttctaaaacc aaactattat catcgcgaaa aaaaccctgt tgccagatgg
    17821 gtatttaaaa agctgagtat tcccaatgga ataatcatct ttaaaactgt gctaatggct
    17881 attttgattt tggctatttc ctattatacg gcttgggatt cgttaactat aaatatagcg
    17941 atgactgttg cggatctcct ttttctgatt gtagttatac acaattaccg tctttaccgt
    18001 cggataaaac attagtcttc tctgcttact aagcaagcaa gtaatttttg gtatcagatg
    18061 ttcctaaggg tggcaattat aaatatttct ggattttacc ttctattaat cctttaattt
    18121 cattcatcct ttcttctgta attgcttcat aacgcgtaac gattattggg gttgtattgg
    18181 aagcgcgaat caaaccccat ccatccgaaa aagttatcct ggcaccgtcg atatcattta
    18241 cttcatagcc ttctttctta aattcttcac aaaccttggc tactacttcg aatttttttg
    18301 catcgggaca gcttgtatgt agttctggag tattaaaaag tttaggttga tcagccaaaa
    18361 aagaactaac cggatttaga gtttgcgcta caatttcaca aaaacggcag cttacataga
    18421 tggcatcatc aaatcccaaa tatctatctg ccaaaaagat atgcccactc atttcacctg
    18481 caaattccac tttcttttcc tgcatataca tctttatatt ggcatggccg gttttataca
    18541 taatcggttc accgccatac tttataatgt cgttaaatag attcatactg catttcacat
    18601 cggcaataat cttcttaccg gggttatgtt tcagataatc cctggctagt atattcaata
    18661 tctgatctcc taacaataat ttaccctgtt catccacaac tccaatgcgg tctgcatctc
    18721 catctaaacc aatgccaagt tcatatttat tttctataac ggctttagat agcgcttcca
    18781 tatttttggc aatagtagga tcaggatgat gattaggaaa atccccatcg ggctcacaaa
    18841 acatatctat cacttcacaa cccaaacggc gtaaaatagc tgttaaataa ggtccgccaa
    18901 ctccatttcc tccatctaca atcactttaa ccggacgctg taattttatt ccattcacta
    18961 cataatcaat gtaaatatca cccatatcat tgtttttaac ataattaccc tggccagaaa
    19021 caaaatctcc cttttgaata atttgtaaca ctttttgcaa ttcatcagca taaacactgc
    19081 ttaaaccaag atttaattta catccattat aacgaggagg attatgacta gctgtaacca
    19141 ttgctccgcc atccgttttt agcttccaga tggaaaaata taaaacagga gtaggaacaa
    19201 ttccaagatc aataatatca caacctgttt ctaaagcacc tttactaaat gcctgcataa
    19261 aagagggggt tgaatgtctg gcatcaccac ccaaaacaat agtttttagg tttttattcc
    19321 gtatataggt tccaaaaccc ttaccgatta aataaaatga attctcatcc agttcttcat
    19381 ttacaattcc acgaatgtca tactgacgga atacttctgc tttgatcatt gataaactcc
    19441 tttaatcagt caataattac tttctccaaa atgggtataa tagctttcat cgccaaggcg
    19501 cgatgggaaa ttttattttt ctgttctgaa tccatttctg cgtaggtttt atctcccaca
    19561 gcaaatatac tgtcgtaacc aaaaccgtta tcgccaagtt cttcagtggt tatttcacct
    19621 tccagttttc ctcccagaac agcaacaata ccatcaggaa gagctaaagc tacaactgtt
    19681 tcaaaatatg ctttacgatt ttctacgccc tgtaacaggt ttagtacttt tttacgatta
    19741 tcagaatagc tgcaagaatt accggcaaag cgagcagata aaatacctgg agcaccattt
    19801 aattcttcaa taaataatcc ggtatcatct gctaaagtaa gtaaaccgct atattttgcc
    19861 gcttccaaag ctttttgcat cgcattttcg taaatcgttt taccattttc ctcgctggga
    19921 agaaattgag gtaattcatc caaaaaatgc aattgtacct tcaggttatt tagtagttcc
    19981 tgcagctctt ttagtttatc aggattgtga gtagcaacta ggatatcact aatcatatct
    20041 tttttaacac attcttgatc ttattcaata aatgcttatc caaaaattgc tctgctaaag
    20101 ctgtaatctt ttttaagtgc tccagaactt caccttcgcc actccaatta tctaacacaa
    20161 tttgaggatt aagttcattt aaaatcatat tcaaaccaag aacaattaaa accagatcat
    20221 ccacaaaacc caaaaccggt ataaaatctg gtataaggtc tatcggcatc atcacataag
    20281 caataacgcc tcccatcagt aatttttgtt ttacgctaac ccgtttatca acagctatgc
    20341 ggcaaaccaa aatgaaaaaa tctggtaata aaaatacata ttcacccagt tttccaccta
    20401 agggacctaa ttgggtatta gtccaactcc ttactttctt acgcagatct tcgtaaaact
    20461 gtaatttcgt ggaatctaca tcatcatcga tgattttatc tttaatcttt tcctgctctt
    20521 caacaggaat ttccttaccg atgattacat catcttcttt cataataaac ctccttttac
    20581 ttatataata atgataagtt tcaggtcaag caaattattt tggatccaac agtttgcgct
    20641 tattataaaa aagggggata tcattttcca ggttggttga tatccccgtc tataatttct
    20701 aagttacctt atctactaat agtaaccata tatcctaatt ataattgaac cacaaagatg
    20761 tagttcattc aatttattca ctaatagcag ctttggctgc agcaacacga gcaataggaa
    20821 ctctataggg tgaacagctc acatagttca ttcccaccat atggcaaaat tctacactac
    20881 taggttcgcc accatgttct ccacaaattc caattttcaa ttcgggacgg gtttttctgc
    20941 cgcgttcaac cccgattttt actaattggc caacaccttc tctatcgaga acttggaaag
    21001 gatcttgttt cagcagattt ttctctatat atatcggtaa aaacacacct gcatcatcgc
    21061 gagaaaaacc aaaggtcatt tgagttaaat cattcgttcc aaagctgaaa aattctgcat
    21121 gttcagcaat tttatcagca agtaaagtag cgcggggaat ctcgatcatt gttcccacca
    21181 gataatccac tctatcattc atatcagtaa aaactttttc ggctgtttca cggatgatag
    21241 tttcttgcat ttcaaattct gcagggatac ttgttaaagg gaccataatt tctggtaaaa
    21301 cattaattcc gcgggcttta acagctaaag cagcttccat tattgccctg gattgcattt
    21361 cggtaatttc aggataggta tttcccaatc gacaacctct atgcccaagc ataggattaa
    21421 cttcatgtaa agaagcaact ctggctttaa ctatttccag cggaattcct aattctttag
    21481 caatttcctg ttgagcttct tcttcttgag gaacaaattc atgtaggggg ggatccaaaa
    21541 gacggatagt tacaggaaaa ccattcatag cagtgaagat accttcaaag tcactcctct
    21601 gcataggtaa taattttttc agtgcctctc ggcgtccttg ttcatcttca gctaaaatca
    21661 tctcccgcat cgagttaatt cgttcgcctt caaaaaacat atgttcagtt ctgcacagtc
    21721 ctattccttg agcaccaaaa tgtctggcta cagatgcatc ttttggagta tcagcatttg
    21781 tgcgaacttt cattctggta tatttatctg ccagttgcat aatagctcca aaatcaccac
    21841 tcagttcagg atcttgtgta tctattttac cttctaaaac ctgaccagtt gagccgttta
    21901 atgaaatcca gtcaccttct ttatatgttt ttccatttac agtcatagtt ctgtttttat
    21961 aatcaatatg taattttcca gcaccagcaa cacaacattt acccattccg cgggcaacaa
    22021 cagctgcgtg agaagtcata ccacctctgg cagtaagaat accttgagcg acagacattc
    22081 cccttaaatc ttcaggtgaa gtttcttcgc gaactaagat aactttttta ttattttcag
    22141 cccaggcttc agcatcatct gcaaaaaaga caatctgtcc agtagctgca ccaggtgaag
    22201 caggtaaacc taaagtaata acttttgcct tagccagacc ttctttagta aaaactgggt
    22261 gtaaaagttc atcaagtttt gcaggttcaa tacggtttag agcagttttt tcatcaataa
    22321 ttccttcgcg taacatatcc atagcgattt tcaccattgc tgcacctgtt ctttttcccg
    22381 ttctggtttg taagagccag agcttacctt cttggatagt aaattcgaga tcttgcattt
    22441 cgcgataatg attttccagc tttttctgaa tttcgtacaa ctctttatag gtttcgggca
    22501 ttgtttcttc taaagatgga tatttttcct ttctttcttc ttctgagata ccggcaagtt
    22561 ttgcccagcg ttgagaacca attttggcaa tctgttgagg agttcttatt ccagcaacta
    22621 catcttcgcc ttgagcatta attaaatatt caccattaaa taaattttca ccggtagcag
    22681 catcgcgtgt aaaggctacc cctgttgcac ttgtataacc catattgcca aaaaccattg
    22741 cttgaactgt tacagcagtt ccccattcat caggaatatt attcagttta cgataaaaaa
    22801 tggcacggtc attattccaa ctatcaaaaa cggcaaaaat tgcaccccaa agctgttccc
    22861 aagggtcatc aggaaacttt ttatgtattt tttcctgcac ttcttgttta aaaagtgtta
    22921 ctaattgttt aagatcttcg gtagttagtt ccagatcgct gcttattcct ttttcttttt
    22981 tcacctgatt aataataact tcgaagggat catgttcttc tttattggca ggcttcatac
    23041 ctaaaaccac atcagaatac atttgcacaa aacgacgata gctatcccag gcaaaccttt
    23101 cattattggt ttt
//
LOCUS       DAORHB010000002        20992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_1721_length_20992_cov_5.649950, whole genome shotgun sequence.
ACCESSION   DAORHB010000002 DAORHB010000000
VERSION     DAORHB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 20992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20992
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_20992_cov_5.649950"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..827)
                     /locus_tag="PKM80_00080"
     CDS             complement(360..827)
                     /locus_tag="PKM80_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92332.1"
                     /translation="MVEKLKELYPEKSKCWKPGVTNAQENNRTFNIICDKNIKKTIVK
                     VWIDGCLIPDQDKKKCDYMFIIPEGKVLIMVECKGNDVKKAIEQIRSTIQILKPLVDL
                     SSQKYAFIVPTMVAPAIQSNIQIVMKKFMREYGIELIVKSHHCDFNLANKKVN"
     gene            complement(834..2093)
                     /locus_tag="PKM80_00085"
     CDS             complement(834..2093)
                     /locus_tag="PKM80_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV92333.1"
                     /translation="MNTLTIKNFGPIKDISLNLKKVNLLIGTQGSGKSTIAKILSIMN
                     DPELVMADTLDKFLVDYNINYDINENTFMEYKTPEFNVTIESNQIKHSLKKTLNDEEL
                     RKFVLSKLKISINDINKWFNTPKESLKSESNEIEQDLINKGFEITSDKIEDTQEYIKF
                     VFNYPIIQLASTLFLESCIYIPSERNFFALLAENIFGLLAENIISSGKKDMTVLPKCI
                     LKFGRYFEQAIKSIKKDDAVDIPFIEELKFIRREGRNEIIYKGVSNSLTQGSSGFQSS
                     IPLAIVCLQNKNSDRSLFIIEEPEQNLYPVTQYNMVKFLAENCLNQNNKLLITTHSPY
                     ILTSFVNLIQAHSSGAIHPKLTAKLIPKTQWIDFNDVSAYFIDKGSAKDILDYEEKTI
                     FAEEIDAASSDIANEYNQLLEIAYRNR"
     gene            complement(2156..3094)
                     /locus_tag="PKM80_00090"
     CDS             complement(2156..3094)
                     /locus_tag="PKM80_00090"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNV92334.1"
                     /translation="MNLAGIQNFRKSRKGKVILYFLKLALSVLILWQIFKKVDLFSAL
                     KDITSLPAYIILLLIAISFLRHTLQFHNWYYSLKLHSKFREKKKSVVSSYFVGQTLRF
                     AVPGGPASFAKIFYVPNNSVLASFWSSIAEKSVMTWVTWTFAIIAGIFYYSKFPLWIW
                     IIVLLVLLSFPFLIKLFLGLSKNYATMKPLYAIQIPKVIAVQTFSTILCYVQYWLILN
                     CLLPLSFWQTFIRMALTQFSNTIPITISGLGLREGFAIHFLAGAGFSAEQAVSTTLTL
                     FFIHDVLPALIGIFFLLQGKKNKSRQAGQGKDIDEI"
     gene            complement(3098..4555)
                     /locus_tag="PKM80_00095"
     CDS             complement(3098..4555)
                     /locus_tag="PKM80_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNV92335.1"
                     /translation="MNELNANLSNLAEVFSELFAPENQSAAQTYFLKELSLKMHSFYQ
                     GKMQTLPKAGIWGFNWFNVWYTPGVSSVSTTIRDNPLKSYELSNRGNLVAVVSDSTRV
                     LGDGDCGIYGGLGVMEGKAMLMKYLGACDAISLCIDSRNEEGKPQAQKIIDFVKMLQP
                     SVGAVNLEDISQPNCYLVLDELRQSCSIPVWHDDAQGTACVTLAGLLNALKLADKDIK
                     KIKCVLFGAGASNTTIASFLMQSGLNPEKLIMFNSKGSLHPKRYDLRDNPAKFKQWKI
                     AQISNPDCQDGIENALTDADVLIALSTPGPDTIKPQWISKMAKKAIVFTCANPVPEIY
                     PYDAKQAGAFIAATGRGDFPNQINNSLGFPGILKGALTVQASKITDNMAIKAAHSLAN
                     FAEKKGINPEYIIPQMDEEDVFAYVSADVATQAIIDNVARKELSWNEVFNIVQREIRE
                     TRSLCLEMMNSGYLKPPDKAIIDGTLQETIMRFGN"
     gene            complement(4845..5741)
                     /locus_tag="PKM80_00100"
     CDS             complement(4845..5741)
                     /locus_tag="PKM80_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNV92336.1"
                     /translation="MDMKTFGYLYYKTRGYAHDDILKNAEITEEEYEFFEKNLQDVLK
                     EIETLRPVAENIGQDFVRLTRYIYERESDQTLGVQRPDAVKARVGEIVSLPAIGTLTM
                     PDMPLAKAIEQRRSRREYSQEALKQEELSFLLWASSWARDFRSTERMELTFRNVPSAG
                     SRHPIETFLDIRRVEGIKPGLYYYHPIKHCLILYDNSEQIAAKIHEGCMFQEMISNAA
                     VNFILTAVPYRTVWRYGQRGYRYLYLDAGHIGQNIHLAAEAIDAGACMIGAFLDEAMN
                     EALGLDGIEEFVIYISSVGKKP"
     gene            complement(5758..6813)
                     /locus_tag="PKM80_00105"
     CDS             complement(5758..6813)
                     /locus_tag="PKM80_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNV92337.1"
                     /translation="MIALIMAGGVGTRFWPKSRKNFPKQFLKVSGELSLLQMTVERLL
                     PQFSFTDIYIVTAQNQVELIKEQLPELPEQNIIIEPFGMNTAPCIALSVMYLKNFYPE
                     QELMFVLPADHIITDKENFLQSLLPAEEQAKKGYLVTFGILPTYPATGYGYIEAGEEI
                     APEVRKVLNFKEKPDKATAEQFLAKGSYYWNSGMFCWSLKSISTAFTNFLPEVDNITK
                     AIANKWQENGLQTDISSLYAKMPRIPIDIGIMEKADFRGVVPVSYDWSDVGSWKALSD
                     LVNKDEDGNYFSAEGKAISATNNYIDTNKFTAIIGVNDLCLIETADAILLCNKEKAEE
                     VKKIVEHLQQVNREDLL"
     gene            complement(6810..7430)
                     /locus_tag="PKM80_00110"
     CDS             complement(6810..7430)
                     /locus_tag="PKM80_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNV92338.1"
                     /translation="MKIAKITPLILFLSCISLLGITSEALHQKIANTYSSFSSFQANL
                     KQENYFAQLNKSITYQGNIYFTKGRMVMRFTKPSFQRLMISGGIVELYDSESKTVFRS
                     KMRPEFGKMNPVEILQLYWKKSSVNVQSSKGDLANVTLKPYSDPIIVSISAVVDTKTG
                     IIQSLSWTNKNNDKVTYNFSNIKTNTKIPASIWQFTYPKDVQVVEQ"
     gene            complement(7417..9663)
                     /locus_tag="PKM80_00115"
     CDS             complement(7417..9663)
                     /locus_tag="PKM80_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNV92339.1"
                     /translation="MANNPKHKTKSHKASQKMSTAKKRTISGIICLLSILVIIALNMG
                     YNSIKNDLESLKAAGSIFSWFQAGNSTTNNPVGIFGIVFGYVFIYLLGFWFSLIGFIL
                     IALISFQYFLAPEAEKGRQKAYLLILVMFLLQVLLVSGQQEYIHSIIPLFVYRILAAV
                     FSGVGARIILIGAMILTVIMLIEYERLKAWISAAAENLQQKKETKIKAPKPTITANNI
                     NPEQTVAEETIPNIQNHTILNPSIDSTPKEFPKPQKENKTKRVFSEGTDKEYQLPAID
                     EFLESPIKLSDREHKEIENQIITTSQILKSKLAEFGIEAEVKNVNIGPIITQYELEPA
                     KGIKVSRFTSLADDLALAIKAKSIRVQAPIPGRGLIGIEIPNLARDMIYLKDLLLSEQ
                     MKQTSSKLAFGLGKDIAGKPIVADLAKMPHLLIAGATGSGKSVCINTIIMSLIMRTKP
                     DDLRLILIDPKRVELAAYNELPHLIGQVVTDADTALETFIWAVREMERRYEILQDARV
                     RDIIGYNEKCEEDEDLEPLPYIVIIVDEFADLIMTSGKDIELPITRLAQMSRAVGMHL
                     ILATQRPSIKVITGIIKANFPARIAFQVSSRVDSRVILDMIGAERLLGNGDMLFLPPG
                     KALPERIHGAFVSDAEIARVCSFLATQPKPTQDFSIVVEDKESLGFFDYDDELFPEAA
                     RVVVSSGTASVSMLQRHFKIGYARAGRLIDLLERARIVGPHLGSKSRDVLATREDLIR
                     IGVIDENR"
     gene            complement(9685..10761)
                     /locus_tag="PKM80_00120"
     CDS             complement(9685..10761)
                     /locus_tag="PKM80_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNV92340.1"
                     /translation="MKAAIIGLPKTGKTTIFNALTRSEHNTDKYAPASLEANIGVVQV
                     ADERIDKLSQLYQPKKTIYATIEYHDYPGIFYKESETSDTALLSDLKTAEAFILVLRG
                     FIDEELEGLYPLGSPETQFQHFQDEMLLADLIMAEKRLEKIELGYKRGVKTPAIQIEE
                     KALRKIVDCLQNNLPLTNLELSSEEEKAIRGFQFLCFKPMMILINCNEDNLTAWDDTL
                     NTFREKGFPAHSIAGKFEEELSKLDNEEAQMFMEDMGISDSIRDRLTHWTYELMGYIS
                     FFTVGPDEVRAWTIEKGSNAVTAAGKIHSDLARGFIRAECFQYEDIINLGSEKALKEK
                     GLFRLEGKDYIVKDGDILSIRFSV"
     gene            complement(10758..11549)
                     /locus_tag="PKM80_00125"
     CDS             complement(10758..11549)
                     /locus_tag="PKM80_00125"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV92341.1"
                     /translation="MKSSSPNSTEPSSKTVSRAGKTIRLNRYLADCGLGSRRKVEEFI
                     TSGRISVNGVIVKDLSTQIFSGKDTVTLDGKIVQPELKKVYLILNKPRGYVVTRSDEL
                     GRKTVYSLLPQQAKELSYAGRLDKDSEGLLLFTNDTILINMLTHPKNKVEKVYRVDIN
                     RSLTNRELNILRNGVEIEGGKTLPAGVFVKAQNIEGMSLKIIISEGRKRQIRQMIEAV
                     DAQVTYLKRLQFGPIKLKKLPVGSWRFLDSAEIQSLLYIKKGKNR"
     gene            complement(11488..11988)
                     /locus_tag="PKM80_00130"
     CDS             complement(11488..11988)
                     /locus_tag="PKM80_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNV92342.1"
                     /translation="MVKKFIYPVLLLLILSSCFYSVYSNAYPHLRKIRILAFENRTSE
                     YGLGDKLLNYLNREIRNDGRLKIVTEDPDCTLEGAILSFSESVYSYDTANQIQDYQLT
                     MVVSVTFTDLINNTVLYENNALTVTELYAVAEGGTAKFKTKEEAVEELIAKLYRTVLQ
                     NSIEGW"
     gene            12084..12758
                     /locus_tag="PKM80_00135"
     CDS             12084..12758
                     /locus_tag="PKM80_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV92343.1"
                     /translation="MAKKFISKAEAAKKLKVSERVIQEMIATNVFESKLVGKNIKIDE
                     DSLNEWLENLNEIDEKMLALKRVICHFEEYMRPENIFLDFTAENKYDAIRILSEKAKD
                     LKLVRDARWLYEVVVAREELVSTAIGNGVALLHPRHLHPSKIKTPSILFGRSSEPVDF
                     DAPDNKPVNIFFMLLLHNDKQHLFSLSYISRLVMDQDVLKRLTEAADATEIHQLLTVI
                     PEQTNK"
     gene            12755..13207
                     /gene="purE"
                     /locus_tag="PKM80_00140"
     CDS             12755..13207
                     /gene="purE"
                     /locus_tag="PKM80_00140"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNV92344.1"
                     /translation="MKPIIRIILGSKSDLDKIAEAKDVLEQFKVPYDLFISSAHRNPD
                     KTIALAQNAKSEGIEVIIAGAGLAAHLPGIIAANTTLPVIGIPFAAGALNGLDSLFSI
                     VQMPPGVPVASMAINGAKNAALFAIQILALKDKELEAKFATYKKDLSA"
     gene            13250..13999
                     /gene="gpmA"
                     /locus_tag="PKM80_00145"
     CDS             13250..13999
                     /gene="gpmA"
                     /locus_tag="PKM80_00145"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNV92345.1"
                     /translation="MYKIVLLRHGESIWNKENLFTGWTDVDLSEKGIVEAQNAGKRLK
                     EEGFVFDEAWTSVLKRAIRTLWIVLDEMDQMYIPIHNDWRLNERHYGALQGLNKAETA
                     IKYGEEQVLLWRRSYDTPPPPLEKNDPRYPGFDPRYANLREDQIPLCESLKDTVKRTM
                     PFWNEIIMPRLAAGRKLIVSAHGNSLRAIVKTLNNISDADIVSVNIPTGIPLIYEFDD
                     NLRPKKNYYLADKAELEKAQQAVADQGKVKK"
     gene            complement(14152..14649)
                     /locus_tag="PKM80_00150"
     CDS             complement(14152..14649)
                     /locus_tag="PKM80_00150"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNV92346.1"
                     /translation="MSIVTKGGDSGKTSLYSGERVFKDDLRVEAYGTLDELNAWISEC
                     RHHLPNSVYKDALREIQQNLSRCMGQLATKNGSFPDPISQNDVDNITEYVYLLEKNLH
                     FDGFVIPGGTPVSAKLDICRTVARRAERRIITLSHKEHIAPELLSYVNRLSDFLFALA
                     RFLET"
     gene            complement(14654..15025)
                     /locus_tag="PKM80_00155"
     CDS             complement(14654..15025)
                     /locus_tag="PKM80_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNV92347.1"
                     /translation="MQSVMTHRAPAAIGPYSQAILMNNTLFVSGQLGIDPGTSSILEG
                     FEEQANLVFNHIKSILDAAGMGMSNIVKVTVFLKDLDDFTLLNDIYTRYFSAPYPARE
                     VVQVSRLPKGGKIEISVIAMR"
     gene            15297..16577
                     /locus_tag="PKM80_00160"
     CDS             15297..16577
                     /locus_tag="PKM80_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNV92348.1"
                     /translation="MVQEPQDFHEFMPNEVWKWKILEEKIEKVLSLHDFQEIRLSILQ
                     DSQILENGIMALVQGKEADSAMKGVLSLANPEDKLSLLSLRPEGTISVLHNTARIANP
                     GDIHRFYYSGPMFRKVDNNQQMEFYQLGVELLGSDSILSENEVIDLGIKLCKTLGLND
                     VRLELNSYGCSNCRRPFFNDLHKYLEEHKSEYCLSCYNELSANPFSQVHCKDPKCKKS
                     LAEGPLIHNYLCKSCKADFRKVKSIQANLGNQYKVNPLLNKNFAYYNKTVFDFIAQNA
                     GKETVIGGGGRYDYLSSLITGKNIPAVGFYLNLDTIFDIMESQGLFATKDPEFVVYIC
                     AESPNLETMTLQIAQELHSQQVKTIISPECKQIKEEIAIAKQNKCPIMIILREDNLRE
                     GKVLIRNLVKEEQNYISLKDTLSTILLARKSMQS"
     gene            complement(17134..19710)
                     /gene="clpB"
                     /locus_tag="PKM80_00165"
     CDS             complement(17134..19710)
                     /gene="clpB"
                     /locus_tag="PKM80_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNV92349.1"
                     /translation="MNTQRFTIKSQELLADMQDLASSYGHQEIKDLHLLQAMLSQEEG
                     LILPALQKLEVSVPKVKSEVENALNKLPKISGSSQQYLSQEVLDILHQAEKEADQFQD
                     DYISTEHLLMALLVKGKEAANILKSEGLQADKLLLALKELRGNQRVTDQSPEAKYQAL
                     EKYTRNLTRLARMEKLDPVIGRDEEIRRCIQILTRRRKNNPVLIGEPGVGKTAIVEGL
                     ARRIVARDVPENLMDKDLLELDMAAIIAGAKFRGEFEERLKAVLSEVEKSEGRIILFI
                     DELHTVVGAGAAEGSVDASNMLKPALARGNLHCIGATTLTEYRKYIEKDAALERRFQP
                     VLVTEPTVEDTISILRGIRDKYEVHHGVQISDNALVAAAQLSERYIADRFLPDKAIDL
                     IDEACALLRMEIDSLPVELDESERRLRQLEIERLSLAKENDPLSQERLEKIKEEIAEL
                     NEENRALRLRWEKEKQVLEQIRALKTEIDTLKGEAEKAEREGNYEKTAQLRYGTIAAK
                     EKELQKLLQNETLTKKDRLLKEIVDEELVAEIVSKWTGIPVSKLAESEMQKLLELENV
                     LAQRVVGQEEGIKALANAIRRSRSGLSDENRPIGSFIFLGPTGVGKTELAKTLASYLF
                     DTEKALIRLDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVLLFDEIEK
                     AHPDVFNVLLQILDDGRLTDGKGRTVVFKHCVIIMTSNIGSQEIYEASDKELERLKPQ
                     LMNILQQYFRPEFLNRVDDIILFHRLDKDHIKKIVKLQLNLLAERVANRNLQLDFSEN
                     LIDHLAEAGYDPAFGARPLKRLIQKEIEDTLARHILAGQFTTGKSIMLDWNPQEGLII
                     TQ"
     gene            complement(19869..20462)
                     /locus_tag="PKM80_00170"
     CDS             complement(19869..20462)
                     /locus_tag="PKM80_00170"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HNV92350.1"
                     /translation="MSYARKSQKRIPRRIYIFTEGSKTEPKYFREFIQYYKISQAQVK
                     VIGRETTASSPKSVIEAMDYYKKSNPVKTKNDIYCMVIDTDRWGKNLKEAVDDAHQRR
                     YLVALSNPCFEIWLLMHFQDANTILNNANLLMTKTEINTQIHSYPITGNNEIDYFPRT
                     DSAVETAEKLDPKPQQRLLEQIGSRVYSLMKILQEYI"
     gene            complement(20459..>20992)
                     /locus_tag="PKM80_00175"
     CDS             complement(20459..>20992)
                     /locus_tag="PKM80_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV92351.1"
                     /translation="ESLRMDLPGNITLKTGERYSMNFKNGIQEAYRILISHKIPATET
                     KVEFKINEESDGTNRIFDLIPVLQLLQEDAIVLVDELDRSLHPLVSRAFVKALFTANK
                     QHQGQFIVTTHDATLLDVKLLRRDLIWFIRKNYQMESTLYSLHDFEKIRNDKALQKAY
                     LAGLYGAVPIIIDEDEE"
BASE COUNT         6422 a         4094 c         3758 g         6718 t
ORIGIN      
        1 tgtagttttg gaaatttgat gaaatatttt ggttgggata ggtctatcta tatgggactg
       61 aagaaaactg cagattattt ggtgatgggg gcaattgcct ttaatttgaa acgcagtata
      121 aaaattttac gagcatagta tagaaggggt ttgcccagat tatgggataa gggtaaaaaa
      181 tgaacaaatt tgagggtttg gggaaggcat aatggtaaaa aacagataaa aaagacctat
      241 tctttgatta tttctcccaa tcagccaaaa atcattgaga taatgccctc gctcctaatt
      301 gttcaatgat cttgtgtatt ccttgcgaga tagcaataat tatagattta ctatagcgat
      361 caattgactt tcttattagc taagttaaaa tcacaatggt gactcttaac tattaactca
      421 atcccatact ctctcataaa ttttttcatt acgatctgaa tattggattg gatggctggt
      481 gctaccatag taggaacaat aaatgcatat ttttgggagc ttaaatcaac caatggtttc
      541 aatatctgga tagttgaacg gatttgttct attgccttct ttacatcatt tcctttgcat
      601 tcaaccatga ttaatacttt gccttctggg atgatgaaca tatagtcaca ttttttttta
      661 tcttggtctg gtattaaaca accatcaatc catactttga ctatagtttt cttaatattc
      721 ttgtcacaaa tgatattaaa tgttctgtta ttttcttggg catttgttac tcctggtttc
      781 caacacttac ttttttctgg gtaaagttct tttaattttt ccaccataat tttttacctg
      841 ttgcggtagg caatttccag caactgatta tattcattcg caatatcaga agaggcagca
      901 tcaatttctt ccgcgaagat tgttttttct tcgtaatcca agatatcttt tgccgatcct
      961 ttatcaatga agtaggcgct aacatcatta aaatctatcc attgagtttt gggaatgagt
     1021 ttagccgtaa gttttggatg tatcgcacca gaactatgag cttgaatcag atttacgaaa
     1081 gaagttaaaa tataaggact atgagtcgtg atcaataatt tattattttg attaaggcag
     1141 ttttcagcaa gaaatttaac catattgtat tgggtaacgg gatagagatt ttgttccggt
     1201 tcttcaatta taaataatga tctatcgcta ttcttattct gtaaacagac aatagccaaa
     1261 ggaattgagg attgaaaacc acttgagccc tgggttaaac tattggaaac acctttataa
     1321 atgatttcat ttcttccttc ccttctaata aactttagct cctcaatgaa aggaatatct
     1381 acagcatcgt ctttttttat ggactttatg gcttgttcaa aatacctacc gaacttcaaa
     1441 atacactttg gcaatacagt catatctttt tttcctgagc taataatgtt ttcagcaagt
     1501 aagccaaaaa tgttttcggc aagtaaggca aaaaagtttc tttcagatgg tatatatata
     1561 caggattcaa gaaataatgt acttgctaat tgaattatag ggtaattaaa aacaaatttt
     1621 atatattctt gtgtatcttc tattttatct gaagttattt caaatccttt attaattaaa
     1681 tcttgctcaa tctcattact ctctgatttt aatgattctt tcggtgtatt aaaccattta
     1741 ttaatatcat taatactaat ctttagttta gagagaacaa attttcttaa ttcttcatcg
     1801 tttaatgttt ttttaagaga atgtttaatt tggttgctct caatagtaac attaaattcg
     1861 ggtgttttat attccataaa ggtattttcg ttgatgtcat agtttatgtt ataatccacc
     1921 aagaacttat ctaaagtatc tgccattact aattcaggat cattcataat ggaaagaatt
     1981 ttggcaatag tgctttttcc actgccctgt gttccaatta gcaagttcac tttttttaag
     2041 ttaagtgata tgtctttaat tggaccaaaa ttttttatgg tgagagtatt catttcattc
     2101 tcctctatca acgcaaagta atatcatttt cgttttttcc tcttataaag gaaggttata
     2161 tttcgtcaat atcttttcct tgacctgctt gccgggattt gttttttttt ccttgcagca
     2221 agaagaaaat accaattaag gcaggcaaaa catcatgaat aaaaaacaaa gtgagagtag
     2281 tggacacagc ttgttcggca gaaaaaccag ctcctgccaa aaaatggata gcaaatccct
     2341 cccgcaatcc caaaccggaa atagtaatag ggatagtatt agaaaattgc gtaagtgcca
     2401 ttctgataaa ggtctgccag aaactgaggg gcaaaaggca gttcagaatc agccaatatt
     2461 gcacataaca gaggatagtg ctaaaagtct gcactgctat aacctttgga atttgaatag
     2521 cataaagagg tttcattgta gcgtaatttt tacttaatcc caaaaatagt ttaatcagaa
     2581 aaggaaaaga tagcaacacc aacagcacga ttatccaaat ccacaaagga aacttggaat
     2641 agtaaaaaat cccagcaatg atagcaaatg tccaagttac ccaagtcatt acactttttt
     2701 cggcaataga actccaaaaa gaggcaagca cactattatt aggcacataa aagattttag
     2761 caaagctagc aggaccaccc ggaacggcaa aacgcaaagt ttggccaaca aagtaagaac
     2821 ttacaactga tttcttcttt tctcgaaatt tagagtgtaa cttcaaagaa taataccagt
     2881 tatgaaattg taaagtatgc cgaagaaagc ttatggcaat cagcagtaaa attatataag
     2941 caggcaagga agtaatatct tttaaggcag agaataaatc caccttttta aatatctgcc
     3001 acagaatcag aacgcttaag gcaagcttga gaaaatataa aatcactttt ccctttctgc
     3061 ttttgcggaa attttggatg cctgccaaat tcattattta gttcccaaag cgcataattg
     3121 tttcttgcaa ggtgccatca ataatagctt tgtccggtgg cttaagataa ccgctgttca
     3181 tcatttccaa acagagggag cgagtttccc ttatttccct ttgcacgata ttaaaaacct
     3241 cattccagct aagttctttc ctggcaacat tatctattat tgcctgtgta gccacatcgg
     3301 cagatacata ggcaaaaaca tcttcctcat ccatttgggg aataatatac tccggattta
     3361 tccctttctt ttcagcaaag tttgctaaag aatgggctgc tttaattgcc atattgtctg
     3421 taattttgct tgcttgaacg gttaaagcgc ctttcaaaat cccaggaaaa ccgagggaat
     3481 tatttatctg attgggaaaa tcgcctctac ccgttgccgc aataaaagca cctgcttgtt
     3541 tagcatcgta aggataaatt tcgggcaccg gattggcaca ggtaaagaca atggcttttt
     3601 ttgccatctt acttatccac tgtggcttta tcgtatccgg accgggggta gaaagcgcaa
     3661 ttaaaacatc ggcatccgtc agcgcatttt ctataccatc ttggcagtca ggattgctga
     3721 tttgggctat tttccactgt ttgaatttag ccggattgtc tcttaaatcg tatcttttag
     3781 gatgcaacga gcccttgcta ttaaacataa tcagcttttc tgggtttaat ccgctctgca
     3841 taaggaaact ggcaattgtt gtattagacg ctccagcccc gaaaagcacg catttaattt
     3901 tcttaatatc cttatctgcc aattttagag cattcagtaa accagccaga gtaacgcaag
     3961 cagttccctg tgcatcatca tgccaaacgg gaattgaaca ggactgcctt agttcatcca
     4021 aaaccaaata acaattcggc tgcgaaatat cttccaaatt cacagcccca acggagggct
     4081 gcagcatttt tacaaaatca atgatttttt gagcttgcgg ttttccctct tcatttctgc
     4141 tatctataca tagggaaata gcatcgcagg cacccaaata tttcatcagc attgcctttc
     4201 cttccatcac tcccaagccg ccataaattc cgcaatcacc atctcccaag acacgagtag
     4261 aatcgctaac cacagccaca aggttacccc gattgctaag ttcataagat tttaaggggt
     4321 tatcgcgaat agtagtagaa acagaagata cgccaggagt gtaccaaaca ttaaaccaat
     4381 taaaacccca aattcccgct tttggcaggg tttgcatttt cccttgatag aaagagtgca
     4441 tttttaggga aagctctttt agaaaataag tttgagcagc ggactgattt tcaggcgcaa
     4501 aaagctctga aaagacctct gccaagttgg aaagattggc atttaactca ttcatttttt
     4561 atcctttata gcattgttgc caggttacaa tattatctat ctgctttttt atgccctaca
     4621 atgaattcct cttccactca ttatcaacac ctttacctct caaccttgag gacgggttga
     4681 tgatgggttg aggaggagag aaggaggagt ggtatattgg aatcaaataa ggaaggcata
     4741 gcaacttatt aattatcatc cagataactt atcaatataa aacagctgca aaagcactac
     4801 cattaagtga tttatattct atacacatag aaataaatat cggattaggg ctttttgcct
     4861 acgctggata tatagattac aaattcttca attccatcca aacctaaagc ttcattcatt
     4921 gcttcatcca aaaaagcgcc aatcatacag gccccggcat ctatggcttc agcggcaaga
     4981 tggatatttt ggccaatgtg gcctgcatca agatagagat aacgatagcc gcgttgtcca
     5041 tatctccata cagttctata gggaacagct gttaaaataa agttgaccgc tgcattggaa
     5101 atcatttctt gaaacataca tccttcgtga attttagcgg caatctgttc ggaattatca
     5161 tacaaaatta ggcaatgctt gatgggatga taatagtata aaccgggttt gatgccttcc
     5221 actcggcgaa tgtccagaaa ggtctctatc ggatgacgcg aacctgcaga aggaacattg
     5281 cgaaaagtga gttccatcct ttcggtagag cgaaaatcgc gagcccagga agaagcccaa
     5341 agcaaaaaag atagttcttc ctgttttagt gcttcctggc tatattctct tctgcttctg
     5401 cgttgctcaa ttgcttttgc taaaggcata tcgggcattg ttagagtgcc aattgcaggt
     5461 aaagatacaa tttctccaac tcttgcttta acggcatcag gacgttgaac gcctaaggtt
     5521 tgatcgcttt cgcgttcata gatataacga gttaagcgga caaaatcttg cccaatattt
     5581 tcggcaacag gtcttaaagt ctcaatttct tttagtacat cctgcagatt tttttcaaag
     5641 aactcatatt cttcttcggt tatttcagca tttttcaaaa tatcatcatg cgcatagccg
     5701 cgagttttat aatatagata gccaaaggtt ttcatatcca tagttttctc tccttgttta
     5761 tagtaggtct tctctgttta cctgttgcaa atgttccaca atttttttca cttcctctgc
     5821 tttttccttg ttacaaagca gaatggcatc cgctgtttca atcaggcaga ggtcgttaac
     5881 tcctattata gcggtaaatt tattggtatc tatatagtta ttggtggcag aaattgcttt
     5941 tccttcagcg gaaaaatagt tgccatcctc atctttatta accaaatcgg aaagtgcttt
     6001 ccagctacca acatcgctcc aatcgtaaga aacagggaca actcctctaa aatctgcctt
     6061 ttccataatg ccaatatcaa tagggattcg gggcatttta gcatacaaag aagaaatatc
     6121 tgtttgtaaa ccattttcct gccatttgtt agctattgcc ttagtaatgt tatcaacctc
     6181 gggaaggaag ttagtaaaag cggttgatat actttttaaa gaccagcaaa acattccact
     6241 gttccaataa taacttcctt ttgccagaaa ttgttccgcg gttgctttat ctggtttttc
     6301 tttaaaatta agcacttttc ttacttcagg ggcaatttct tctccggctt caatatagcc
     6361 ataacctgtg gcgggataag ttggtaaaat tccaaaagta accagataac ctttttttgc
     6421 ctgttcttct gcaggtaaaa gcgattgtaa aaaattttcc ttatctgtaa tgatatgatc
     6481 tgcaggtaaa acaaacatca gctcttgctc gggataaaaa tttttaagat acataacact
     6541 taatgcaata cagggagctg tattcatccc aaaaggctca atgataatat tttgctcggg
     6601 taattctggt aactgctcct taattagttc tacctgattt tgagcggtta cgatataaat
     6661 atctgtaaaa gagaattggg gtaaaagacg ctcaacggtc atctgcaaaa gtgacagctc
     6721 tccgctaact tttagaaatt gtttagggaa gttctttcgg cttttaggcc aaaaacgagt
     6781 gccgactcct cctgccataa ttagggcaat cattgttcaa caacctgcac atctttagga
     6841 taagtgaatt gccagatgga ggcgggaatt ttggtgttgg tttttatatt ggaaaaatta
     6901 tatgttactt tgtcgttatt tttgttcgtc cagcttaggg attgaataat gccagttttg
     6961 gtatctacta ctgctgatat agaaacaatg atgggatcac tatagggttt aagagttaca
     7021 tttgccaaat cacctttgct tgattgaaca ttaactgagg attttttcca atataactgc
     7081 aaaatttcca ccggattcat tttcccgaat tctggacgca tcttgctgcg aaaaacagtt
     7141 ttactttcgg aatcataaag ttctacaatt cccccggaaa tcattaaccg ttgaaaactg
     7201 ggtttcgtga accgcataac cattcgccct ttagtaaaat agatatttcc ctgataagta
     7261 atgcttttgt taagctgagc aaaatagttc tcctgtttta aatttgcctg aaagctactg
     7321 aagctgctat aggtattggc aattttctga tgcaatgctt cactggttat acccaaaaga
     7381 gaaatgcagc ttaaaaaaag gataagcggc gtaattttag cgattttcat ctatcactcc
     7441 tatgcgaatt aaatcttcac gcgtagctaa tacatcgcgt gatttactcc ctaaatgagg
     7501 gccaacaatt ctggctcttt ccaaaagatc aatcaaacgt cctgctctgg catagccaat
     7561 tttaaaatgc ctttgtaaca tagaaacaga agcagttcct gaagaaacaa ctactcttgc
     7621 tgcttcggga aataattcat cgtcataatc aaaaaagcca aggctttctt tatcctctac
     7681 aacaatagaa aaatcctgtg taggtttggg ctgagtagct aaaaagctgc agactcttgc
     7741 aatttctgca tcagatacaa aagcaccatg aattctttct ggtaatgctt tgcctggagg
     7801 taaaaacaac atatccccgt ttcctaaaag tcgctctgcg cctatcatat ctaaaatcac
     7861 gcgggaatct actctggacg aaacttggaa ggcaattcgt gcgggaaaat tagctttgat
     7921 tatacccgtg ataactttga tggaaggacg ctgcgtggct aaaataagat gcatccctac
     7981 cgctctggac atttgagcta aacgcgtgat aggtaattct atatcctttc cactggtcat
     8041 tattaaatcg gcaaattcat ctacaatgat aactatgtag ggaagaggtt ccaaatcttc
     8101 atcttcttcg catttttcgt tataaccaat aatatcacga actcgggcat cttgcaaaat
     8161 ttcataacga cgctccattt cgcgaactgc ccaaataaaa gtttctaaag cagtatcggc
     8221 atcggttact acttgtccaa ttaaatgagg aagttcatta taagcagcca gttcaactcg
     8281 ttttgggtca attaaaataa ggcgcaggtc atcaggtttg gtacgcataa ttaaactcat
     8341 aataatggtg tttatacata cactttttcc acttcccgta gctcctgcga tgagtaaatg
     8401 aggcattttg gctaggtctg caacaattgg ttttccggca atatcttttc ccaaaccgaa
     8461 agctaatttg gaacttgtct gtttcatctg ttccgaaagc aggagatctt ttaaatagat
     8521 catatctctt gccagattgg gtatttcaat tccaattaat ccgcgtcctg gaataggtgc
     8581 ttgaactcta atcgatttag ccttgatagc gagtgccaaa tcatctgcca gagaagtaaa
     8641 acggctaact ttaatgccct ttgcgggttc caattcatat tgagtaataa tagggccgat
     8701 attaacattt ttcacttctg cttcgatacc aaattcagcc agcttactct ttaaaatctg
     8761 gctggtagta atgatctggt tttcaatttc tttatgttcc ctatcggata gctttattgg
     8821 gctttccaaa aattcatcaa ttgcgggaag ctgatattct ttatctgttc cctcagaaaa
     8881 cactcgcttg gttttatttt ctttttgagg tttaggaaat tccttaggtg ttgaatctat
     8941 ggaagggttt aatatagtat gattttggat attgggaatt gtttcttcag cgaccgtttg
     9001 ttcaggatta atattattgg cggtaattgt tggcttggga gcttttattt tggtttcttt
     9061 tttttgttgc agattttctg cggctgcaga tatccaggct ttcaaccgtt catattcaat
     9121 taacataatc accgttaaaa tcattgcacc aattaaaata atccgggctc ccactccaga
     9181 aaaaacagct gccagtatcc gataaacaaa caaaggaata atactgtgaa tatattcttg
     9241 ctgcccagaa acaagcagca cctgcagtaa gaacattact aaaattagca aataggcttt
     9301 ctgcctgcct ttttcagctt ccggagctag aaaatattga aatgaaatga gagcaataag
     9361 gataaatccg attaatgaaa accagaaacc taaaagatag ataaacacat acccgaaaac
     9421 tattccgaaa ataccaacag gattattagt agtggaattg cctgcttgaa accaggaaaa
     9481 aatactgcca gcggctttta aactttctaa atcgttttta atagaattat agcccatatt
     9541 aagtgcaata ataactaata tagatagcaa acaaataata ccgctaatag ttcttttctt
     9601 agcagtgctc attttttggg atgccttatg gctctttgtt ttgtgtttcg gattatttgc
     9661 caatgcgtta tactccttat tctactatac gctaaatctg atagagagaa tatctccatc
     9721 cttaacaata taatcctttc cctccaatcg gaaaagacct ttttctttaa gtgccttttc
     9781 gctgcccaga tttattatgt cttcatactg aaaacattct gcccgaataa aaccccgggc
     9841 taaatcacta tgaatttttc cggctgcagt tacagcattg gaaccttttt caattgtcca
     9901 ggcacgaact tcatcaggac caacagtaaa aaaactgata tatcccatca gctcatatgt
     9961 ccaatgtgtt aaacggtctc taatggaatc actgattccc atatcttcca taaacatttg
    10021 tgcttcttca ttatccagtt tgcttaattc ttcttcaaac tttccggcaa tcgaatgtgc
    10081 agggaaaccc ttttctctaa aggtgtttaa tgtatcatcc caagcagtta agttatcttc
    10141 attacagtta attagaatca tcataggctt gaaacaaaga aattgaaaac cgcgaattgc
    10201 tttttcctct tcggaagaaa gttcaagatt agtaagtggc aaattattct gcagacaatc
    10261 cacaattttt cttaaggctt tttcttcaat ttgaatggct ggagtcttaa ctcctctttt
    10321 ataaccaagc tcgatttttt ccagacgctt ttctgccata atcaaatcgg caagtaacat
    10381 ctcatcctga aagtgttgaa attgcgtttc cggagagcca agtggataaa gaccttccaa
    10441 ttcttcatca ataaagccac gcaaaactaa tataaacgct tcggctgttt ttaggtcgga
    10501 aagtaatgct gtatcagagg tttcactctc cttgtaaaaa atcccgggat aatcgtgata
    10561 ttcaattgtg gcgtagatag tttttttagg ttgatagagc tgggagagct tatcaattct
    10621 ttcatcagca acttgcacaa ctccaatatt tgcttctaaa gaggcaggtg catatttatc
    10681 ggtgttatgt tcgcttcttg ttaacgcatt gaaaatagtt gttttaccgg ttttaggtaa
    10741 accaattatt gctgctttca tctatttttc cccttcttta tatataaaag tgattgaatt
    10801 tctgcagaat ctaaaaatcg ccaactacca acaggaagtt ttttaagttt tatgggacca
    10861 aactgtaagc gctttaaata agtaacttgg gcatcaactg cttcaatcat ttggcgaatc
    10921 tgtctttttc tgccttcact gattattatt tttaagctca tcccttcgat attttgcgct
    10981 ttaacaaaaa cccccgctgg caatgttttt ccgccttcaa tttcaacgcc atttcttaag
    11041 atgttcagtt ctctattagt tagcgagcgg ttaatatcca cacgataaac cttttccact
    11101 ttgtttttgg ggtgggtaag catattaatt aaaatagtat cattagtaaa aagcaaaagc
    11161 ccctcactat ccttatccag gcgtcccgca taagaaagtt ctttagcttg ttgaggtaaa
    11221 agagaataga cagtctttct gcccaattcg tcacttctgg taacaacata gccacgcggt
    11281 ttattcagaa tcaaataaac ctttttaagt tcaggttgaa caattttacc atccaaggta
    11341 acagtatctt ttccggaaaa aatctgtgtg ctaaggtctt taactataac tccatttaca
    11401 gaaattctac cggaagtgat gaattcttca acttttctgc gactgcctaa tccgcaatct
    11461 gctaaatagc gattcaggcg aatggtttta ccagccctcg atactgtttt ggaggacggt
    11521 tctgtagagt ttggcgatga gctcttcaac ggcttcctct ttggttttaa atttggcagt
    11581 accaccttca gcaacagcat aaagttctgt taccgttaag gcgttatttt cataaagaac
    11641 cgtgttatta attaaatcag taaaagtaac tgaaacaacc attgttaact gataatcttg
    11701 aatctggttg gctgtatcgt aactataaac gctttccgaa aaacttaaga tagcgccttc
    11761 caaagtgcaa tctggatctt cagtaactat ttttaaacgg ccatcattac gaatttcacg
    11821 gttcaaataa ttgagcagtt tatcccccaa tccgtattcg ctggttcgat tctcaaaagc
    11881 cagaatccta atttttcgca aatgcggata agcattcgaa taaaccgaat aaaaacaact
    11941 gcttaagatt aacaaaagca gaacaggata aataaatttc ttgaccatta ttccatctcc
    12001 agaaaatgct tatattattg tcaagataaa ccttgcttga acagatataa aatatatata
    12061 aaacctaaaa aagggaggta tttatggcca agaaatttat atctaaagct gaagctgcca
    12121 agaagttaaa ggtctcggaa cgagtcattc aagaaatgat cgcaacgaat gttttcgaaa
    12181 gtaaactcgt aggaaaaaac attaaaattg atgaggattc cttaaacgag tggttggaaa
    12241 atctaaatga aatagacgaa aaaatgcttg ccctaaaaag ggttatttgc catttcgaag
    12301 agtatatgcg tccagaaaat attttcctgg attttacagc cgaaaataaa tacgatgcta
    12361 tccgaatcct tagcgaaaaa gcaaaagacc taaaactggt tagagatgct cgctggcttt
    12421 acgaagttgt agttgcccgc gaagaattgg tctccacagc tattggtaat ggagttgctc
    12481 ttctgcatcc tcgtcactta catccttcca aaattaaaac ccccagcatt ttgtttgggc
    12541 gctcttcgga acccgtagat tttgacgctc cggataataa acctgtgaat atcttcttta
    12601 tgctcttatt gcataacgat aaacaacatc ttttttcgct ttcctatatt tctcgtttag
    12661 ttatggatca agatgtttta aaaaggttaa cagaagccgc tgatgctaca gaaatacatc
    12721 agttgctaac agttattcca gagcagacaa ataaatgaaa cccataatcc gtattatctt
    12781 aggcagtaaa agtgatttgg ataaaatcgc tgaggccaaa gatgttctgg aacaatttaa
    12841 agttccttat gacttattta tctcttctgc acacagaaat ccggataaaa ctattgccct
    12901 ggcccaaaat gccaaaagcg aaggaattga agttataatt gctggagctg gtttggctgc
    12961 tcatttacct ggaattattg cagcaaatac aactctacca gttatcggaa tcccttttgc
    13021 cgcaggagca ttaaatggat tggattctct gttttccatt gttcaaatgc ctccaggagt
    13081 gccagtggcc agtatggcaa taaatggagc caaaaatgca gctctgtttg ctattcaaat
    13141 tttggctctg aaagacaaag aactggaagc aaaatttgct acctacaaaa aagatctaag
    13201 tgcttgaggg actggatgtt taaaccctca gagaaaaaaa ggagttacga tgtataagat
    13261 tgttttactt cgccacggtg aaagtatttg gaacaaagaa aacttattta ctggctggac
    13321 ggatgttgac ctttcagaaa aaggcatagt tgaagctcag aatgcaggta aacgcttaaa
    13381 agaagaaggc tttgttttcg atgaggcttg gacctctgtt ttaaaaagag caatacgaac
    13441 cttatggatc gttctggatg aaatggatca gatgtatatc cctattcata atgactggcg
    13501 gcttaatgaa agacattatg gagccttaca aggtttaaac aaagccgaaa cagctatcaa
    13561 atatggagaa gaacaagttt tactttggcg tcggagttat gatacacctc cacctccttt
    13621 ggaaaaaaat gaccctcgtt accctggttt tgatccccga tatgcaaatt tgagggaaga
    13681 tcaaattccg ctttgtgaat cattgaaaga tactgtaaaa cggacaatgc ctttctggaa
    13741 cgaaattatt atgcctcgtt tagcagcagg cagaaaattg attgtttctg cgcatggaaa
    13801 tagtttacgg gcaattgtaa aaactttgaa taatattagt gatgcggata ttgtaagcgt
    13861 gaatatccca acgggaattc cgcttatcta tgaatttgat gataacttac gccccaaaaa
    13921 aaattattac ctggcagata aggcagaatt ggaaaaagca caacaagcag ttgctgatca
    13981 agggaaggta aaaaaataaa accagacctt ataagctagt aattacatat cctgtaagtc
    14041 aggagtcatt ttgtgcttct atcacatatt tcctatgtga taattgtaat aacacattga
    14101 acttatcaaa gcgatagtaa tgactcctga tttaccgaca taaaaacaaa attaggtctc
    14161 cagaaatctt gccagagcaa aaagaaaatc tgacagccga ttaacatagc taagtagttc
    14221 aggggctata tgttccttat gcgaaagggt aataattctg cgttctgctc ttctggcaac
    14281 agttctacaa atatcaagct tagcagaaac aggtgttcca cctgggataa caaaaccatc
    14341 aaaatgcagg ttcttttcca gtaaataaac atactctgtg atattatcaa catcattttg
    14401 ggaaattgga tcaggaaaag atccattttt tgtagccagt tgtcccatac accgggaaag
    14461 gttttgttga atttccctaa gagcatcctt atatacagaa ttgggaagat gatgtctaca
    14521 ttcacttatc caggcattta gttcatctaa agttccatag gcttcaactc gtaggtcatc
    14581 tttaaaaacc ctttcacctg agtatagaga tgttttcccg ctatctccac ctttagtaac
    14641 aatgctcatt tagttatctc attgcgataa cggagatttc aattttgccg cctttaggta
    14701 aacgagaaac ctgaacaact tcgcgagcag gatatggtgc actaaaataa cgggtataaa
    14761 tatcgtttag aagagtaaaa tcatccaaat ctttcagaaa aacggtaact ttcacgatat
    14821 tcgacatccc cattccagct gcatccagaa tagatttaat atgattgaac accaaatttg
    14881 cctgttcttc aaaaccttcc agaatactag aagtgccagg atcaatacct aattggccac
    14941 taacgaacag agtattattc ataagtatgg cttgcgaata gggacctatc gcagccgggg
    15001 cacgatgcgt cataacagat tgcattttat gctccttaca tttttttgct attatcccta
    15061 tgcagtaata tctgtcaagt ttcataataa caaaaccgaa aacaaaagtt cattgaatta
    15121 gcgataagtg ccggtaacgg taagaatatt tatatatccc gataatccta aaacttctaa
    15181 agtcctcttc tctaaaacaa aatctgcaca tataggtgag agcgatagta aaaataaatt
    15241 gccaattctg taatcttgtt tatatttaat agtaaaggat atgtgaggag taaaagatgg
    15301 ttcaagaacc ccaagatttc catgagttta tgcccaacga agtttggaaa tggaaaatac
    15361 tggaagaaaa aattgaaaag gttttatctc tgcacgattt tcaagaaata cggctttcta
    15421 ttttgcaaga ttcgcagata ttagaaaatg ggataatggc tttagttcaa ggtaaagaag
    15481 ctgattcagc aatgaagggc gttttgtcgc ttgctaatcc tgaagataaa ttaagtttgc
    15541 ttagtttgcg tcctgagggc acaatcagtg ttttgcataa tactgcgcgg attgccaatc
    15601 cgggtgatat tcatcgtttt tactattcag gccctatgtt tcgtaaagtg gacaataatc
    15661 agcagatgga attttaccag ctgggcgtag aactattagg tagtgatagt attctatccg
    15721 aaaatgaagt tatcgatttg ggaattaaac tgtgtaaaac acttggtttg aacgatgttc
    15781 gcttagaatt aaattcttat ggctgttcca attgtcgaag accttttttt aatgacctac
    15841 ataaatatct ggaagaacat aaaagcgaat attgcctttc ctgctataat gaactttcag
    15901 ctaatccttt cagtcaggtg cattgcaaag acccaaaatg taaaaagtcc cttgccgaag
    15961 gtcctctgat ccataattat ctatgcaaat cctgcaaagc tgattttaga aaagtaaaga
    16021 gcattcaggc aaacttaggg aatcagtata aagtaaatcc tcttctaaat aaaaactttg
    16081 cctattataa caaaaccgtc tttgatttta tcgctcaaaa tgcaggaaag gaaactgtta
    16141 tcgggggcgg aggacgctat gattatctgt cttctctaat tacaggaaaa aacatccccg
    16201 ctgttggttt ctacctgaat ctggatacca tctttgatat tatggaaagc caaggattgt
    16261 ttgcaacaaa agatcctgaa tttgtagttt atatctgtgc cgaaagccca aacctggaaa
    16321 cgatgaccct gcaaattgcg caagaactac attctcaaca agttaaaacc atcatctctc
    16381 cggagtgcaa acaaataaaa gaagaaattg ccattgctaa acaaaataaa tgccccatta
    16441 tgattatttt acgcgaggat aacctccgcg aaggaaaagt tcttattcga aatctggtga
    16501 aagaagaaca gaactacatc tctttgaaag ataccctgtc cactatcttg cttgcaagaa
    16561 aatctatgca aagctgaaaa tcacgcaaca ccgaaaagaa tatctacttt attttgtggg
    16621 gtgataagtt aggcgacttt gtccactcag agcaaaagca aggtaagctt gcagtattga
    16681 atatatagta aatcttgata ataccctcct tcggtactgt tcgtttggtg taaaaattta
    16741 cccatccagt tatctgggtt ctaatttttc tatcgctttc caaggtaatc atcctgtatc
    16801 ttgcttagat agttgtggtg gagataccgt ggagctggtg gggaaaaaac cgatagacac
    16861 caccctatct gctccataac taatcaagca aggataaggt tattttatag caatagaatc
    16921 aattagagag tatatgtggc aaagggactg ccggatgttg atttgtaaaa tgcggaacgg
    16981 cataatattg cagttgattt ttcatctgtg atctatcgga gattaataac ccgctttttg
    17041 ctaactacgc aatttgagaa aaaataaggg ctggtatcta actttgttca gtttgcttgc
    17101 cagcccttag agctaagtgt gttatttatt cgtttattga gttatgatta aaccttcttg
    17161 gggattccaa tccagcataa tactctttcc ggtggtaaat tgtccggcca agatatgtct
    17221 ggctaaagta tcctcaattt ccttttgaat aaggcgtttt agcggtctgg ctccaaatgc
    17281 tggatcataa ccagcttctg caagatggtc aattagattt tcagaaaaat caagctggag
    17341 attgcgattg gcaactcgct ctgccagcag gttcaattgc agttttacaa tctttttaat
    17401 atgatcctta tccaaacgat ggaagaggat aatgtcatct acccggttta agaactctgg
    17461 gcggaaatat tgctgtaaga tattcattaa ctgtggtttt agccgttcca gttctttatc
    17521 cgacgcttca taaatttcct gcgaaccaat attgctggtc ataataatca cacagtgctt
    17581 aaaaacaact gttctgcctt taccgtcggt aagccgtcca tcatctaaaa tttggagcaa
    17641 aacattgaaa acatccggat gggctttttc aatttcgtca aaaagcaaca cagaataagg
    17701 tctgcgtcgg actgcttccg ttaaataacc gccctcttca tagcccacat atccaggagg
    17761 tgctccaatg agtcgagcaa cactatgttt ttccataaat tcactcatat ctaagcgaat
    17821 taaggctttt tctgtatcaa atagataact tgccaaggtt tttgcaagtt ccgttttacc
    17881 aacaccggta ggacctaaaa agatgaaaga accgatgggt ctgttttcat cgcttaagcc
    17941 actgcggcta cggcggatag cattggccag agctttaatt ccttcttcct gtcctacaac
    18001 tctttgggca agaacatttt ccagttcaag tagtttttgc atctcgcttt ctgctaattt
    18061 ggaaacaggg atgcctgtcc atttggaaac aatttccgct accaattctt catctacgat
    18121 ttctttcagt aatcggtctt ttttggtgag tgtttcgttc tgtaacagtt tttgtagttc
    18181 tttttcttta gcggcgatag taccgtaacg aagttgagcg gttttttcat aatttccctc
    18241 gcgttcagct ttttctgctt cacctttcag agtatcaatt tctgttttca aggcacgaat
    18301 ttgttccaaa acctgttttt ctttttccca acgaaggcgt aaagctctat tttcttcgtt
    18361 taattctgca atttcttctt ttattttttc cagccgttcc tgtgataagg ggtcattttc
    18421 ctttgccaaa gaaaggcgtt caatttccag ttggcgtaac cgtctttcag attcatcaag
    18481 ttctacaggc aaggaatcaa tttccattct taataaagca caagcttcat caattaagtc
    18541 tattgcttta tcaggtaaaa aacgatctgc aatataacgc tctgaaagct gagctgcagc
    18601 cactaaggca ttatcactaa tttgaactcc atgatgaact tcatatttat cccggatgcc
    18661 tcttaaaatg gaaatcgtgt cttccactgt aggttctgta actaaaacgg gctgaaaacg
    18721 cctttccaaa gctgcatcct tctcaatgta ctttctatat tctgttaagg tcgtggctcc
    18781 aatgcaatgc aaatttcctc ttgctaatgc tggttttaac atattggaag catctacgga
    18841 accttctgca gcaccggctc caacaactgt gtgcaactca tcaataaaaa gaataatgcg
    18901 tccttcggat ttttccacct ccgaaagaac cgctttcaaa cgctcttcaa attcgcctcg
    18961 gaattttgct ccggcaatta ttgctgccat atcaagttcc aacagatctt tgtccattaa
    19021 attttcggga acatcgcggg ctacaattct tcgggctaaa ccttctacaa tagcggtttt
    19081 tccaactccc ggctcgccaa ttagaactgg attattcttc cttctgcgag ttaaaatctg
    19141 aatgcaacgc cgtatttctt catcgcgacc gataaccgga tccagctttt ccattctcgc
    19201 taaacgagtt aaattacgcg tgtatttttc caaagcctga tattttgctt ctggactttg
    19261 atcggttacg cgctgattac cacgcagttc tttaagcgct aaaagtaatt tatcagcttg
    19321 taaaccttca cttttaagaa tattcgctgc ttctttccct ttaactaaaa gagccataag
    19381 aagatgctcg gtagaaatgt aatcgtcctg aaactggtct gcttccttct ctgcctgatg
    19441 taaaatgtcc aaaacttcct gagatagata ctgttgagaa ctaccactaa tcttgggaag
    19501 cttgtttaag gcattttcta cttcactttt cactttggga acagagacct ccagcttctg
    19561 taaagcaggt aaaatcaatc cttcttcctg gctcaacata gcctgtaata aatgcaggtc
    19621 tttaatttcc tgatgaccat aactggaagc taaatcctgc atatcagcaa gcaactcttg
    19681 tgactttatt gtaaatcttt gtgtattcat atatacctcc agtataagta aaatataatc
    19741 gagtttgagg gaatgtcaag ggaaatatta gcagaccaac tcttcgactg ctaattatct
    19801 aacaagtaac caaccctgaa cgaatataac aaatcatctg gagattggaa taaagaataa
    19861 ataggcaatc atatatattc ttgtagtatt ttcattagtg aatacactct ggaaccaatc
    19921 tgctctaata atctttgctg cggttttgga tcaagttttt cagctgtttc tacagcggaa
    19981 tcggttctgg gaaagtaatc aatttcatta ttgccagtaa taggataact atgaatttgt
    20041 gtatttatct ctgtttttgt cattagcaga ttagcattat ttaaaattgt attagcatcc
    20101 tgaaagtgca tcaaaagcca tatctcaaaa caaggattgg acaaagcaac taaatatctt
    20161 ctttgatggg catcatcaac agcttccttt aagtttttac cccatctatc ggtatctata
    20221 accatacaat aaatatcatt ttttgtctta acggggttac ttttcttata ataatccatc
    20281 gcctcaataa cagattttgg actggaagct gtggtttctc tgcctattac ttttacttgt
    20341 gcctgcgaaa ttttgtagta ttgaataaat tccctgaaat atttcggttc cgttttgcta
    20401 ccttccgtaa aaatatatat gcgcctgggg attcttttct gagatttacg ggcataactc
    20461 attcttcatc ctcatcaatt ataattggaa cagctccgta taaccctgct aagtatgctt
    20521 tttgcaatgc cttatcattt cttatctttt caaaatcgtg aagtgaatat aatgtggatt
    20581 ccatttgata attctttctt atgaaccaga ttaaatccct gcgcagtaat ttaacatcca
    20641 ataaagttgc atcatgggta gttactatga actgaccctg atgttgctta tttgcagtaa
    20701 atagagcttt aacaaatgct ctggatacta aaggatgtaa actgcggtca agttcatcta
    20761 caagcacaat agcatcttct tgtaataatt gtaaaacagg aattaagtca aaaatacggt
    20821 ttgttccatc ggattcttca ttaattttaa attctacttt cgtttccgtt gcaggtattt
    20881 tgtgagatat aaggattcta taagcttctt gaataccatt tttaaagttc attgaatagc
    20941 gttcaccagt tttcaaagtt atgttcccgg gtaaatccat tcgcaaactt tc
//
LOCUS       DAORHB010000003        17414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_2367_length_17414_cov_5.287920, whole genome shotgun sequence.
ACCESSION   DAORHB010000003 DAORHB010000000
VERSION     DAORHB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17414
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2367_length_17414_cov_5.287920"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1827
                     /locus_tag="PKM80_00180"
     CDS             292..1827
                     /locus_tag="PKM80_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNV92352.1"
                     /translation="MLKVSIAPDGKEYPLPIEEDAEKERETLKRITAEQRSLGRKIVA
                     VQGLGFVGCVMATVVADATDKDGNPIYFVHGHQRASQRSYWKVPVINNGVPPINSSDP
                     EVPEIFHRTVVEKKNFRATSEESVYSLVDIVVVDIQLDATKPAFGDAEKGYCDITAFR
                     EGIRTLGKYIQPECLVLVETTVPPGTCEQVVKPILEEEFTKRGIDINNQPPLIAHSYE
                     RVMPGAHYVESIRNFWRVYSGVNTKSIELCREFLSNVLDVEHFPLTQLDSTNASELAK
                     TMENSYRAVNIALTLEWAKFAEQIGVDIFKVREAIRKRKGTHDNLLRPSLGVGGYCLT
                     KDPVLANWAMNSLFGVEGTLDFAIKAVNTNDTMPLHTIDIVKSVYPELKNLKIAVLGV
                     SYLQDVGDTRHSPSKILVEFLRKDLAIVKTHDPYVESWPEMEENHIQSDLNNVLPESD
                     VVIFAVGHSQYRNLDPEQLLAICKTKPLIVDCSNFLSDETINKYKALGCAVRGVGKGH
                     IID"
     gene            2055..4505
                     /locus_tag="PKM80_00185"
     CDS             2055..4505
                     /locus_tag="PKM80_00185"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV92353.1"
                     /translation="MILTDAQLFSEISRCEYCENKPCKTACPCDCSPADFMMALAKGR
                     PSDFKRAAGIILASNPLGGICGNVCPDYHCVDACSHKKFDKPIQIPDVQATIIRKARE
                     LDLVPEFSSPIRLNTKIAIVGAGPSAIGAAVTLNQFGYETRMFDPEPLGGQINLISEE
                     RLEEIDIIADLNFVVKTFRLQHIPKKIEKPEELLKEGYAAVIVASGLNVPIRLNIPGD
                     ETAIYGLDILSNPSKYNFSGKRIAIVGGAIAADIALVAAKNSAAYVEMITLENFVEMP
                     ITDREKATLIEAGIEFSPRTRISEIVNENGHLKGIKTKKVYLPKNEKFAPNLIKDEAG
                     VIDNYRIFDMVIIAIGNRATFNVHELERKFDNIFCCGDMVNGPTTVVEAVASGKNTAI
                     KVHLSLSNKAIEKPEKETKSCFPVWGWKKYPVSLETDFFGRIMDSPFILSAAPPTDGF
                     EQMQKAYQAGWSGGIMKTAFDDQDIHIPGEYMFTFGDKKKTFANCDNVSEHSLNRVCK
                     EIARLIKEFPDRLTMASTGGPVTGNDCSDKQCWQSNTKKLEQAGAMGIEFSLSCPQGG
                     DGTKGDIVAQDPVLTAKIVDWVMEISAPEIPKLFKLTGAVTSIYPILSAIKNVLNKYP
                     HKKAGITLANTFPALAFREKEKPWDEGIIVGMSGEGVLPISYLNLARAGNQGIFISGN
                     GGAMNYLDAANFLALGARNVQFCTIVEKYGYNIIEELKMGLSYLLEARGLKSVAELVG
                     IAQPNPIIDFMNLPKKIKSSSLNRELCLKCGNCTRCPYLAIELDEDCFPKIDNDQCVG
                     CSLCTQLCFTGALQMKKI"
     gene            complement(4578..5327)
                     /locus_tag="PKM80_00190"
     CDS             complement(4578..5327)
                     /locus_tag="PKM80_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV92354.1"
                     /translation="MNKNLFVLTLLLTIILLVASCSPEQKKILRVGTDAENPPFTYLE
                     NSEFKGIDIDICEKIADKMGMQLQITQYEFDDLFSALAQNKIDLAASTITITEKRKQT
                     FEFSEPYFQTNLAVVARKASPIEINKIEDIGKYRVGCQSSTTSYQYLKENLVDKDLLP
                     KEKLKTYSTLIETIGELLNKKIDLMVFDEWVAKKVSEQVDIKIVYIIPTNEQYGLAMR
                     PGKEINLRINKALKELKDSGELQRIINEYIK"
     gene            complement(5432..6214)
                     /locus_tag="PKM80_00195"
     CDS             complement(5432..6214)
                     /locus_tag="PKM80_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425332.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNV92355.1"
                     /translation="MFTVLLVLICIAIALCLGWIIFAKDKAISRTWGIVGLSFVIGLT
                     VFGYFFMQSRQAPTSLRVVGYASKLFESDIVKWNLTMQRNVSNDGLKDGYNKMSADVN
                     AFKKYLLEQGIPEKDISIQPTNSYQTNDNYGNPTGYCLNQRIFVVSTDIDKIEKLSLN
                     PDFFAERGILLQNSNLAYLYTKLPDLKKQLLSEATKDAVARAEEISGSGNTKLGKLRE
                     ARAGVFQITEPYSTEVSDYGFYNTSTRSKSISVTLTAIFKLK"
     gene            complement(6226..6663)
                     /gene="tsaE"
                     /locus_tag="PKM80_00200"
     CDS             complement(6226..6663)
                     /gene="tsaE"
                     /locus_tag="PKM80_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNV92356.1"
                     /translation="MITINLPSEQDTIDLANYLAPILKAGDIITLYGDLGSGKTFFVK
                     HLGKELGINEEIDSPSFIIMKEYLGGRLPLYHLDLYRLKDKEELYDLGIFDILEKGVT
                     VIEWPHIAESILPYQTFKLDFHFDGKSRWVNVFPDDDHKQYFE"
     gene            complement(6697..8265)
                     /locus_tag="PKM80_00205"
     CDS             complement(6697..8265)
                     /locus_tag="PKM80_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV92357.1"
                     /translation="MKQMHILWVDDEIDLLKPFILFLEERNYLVDTCNNGTDAIEKVV
                     ENKFDLVILDEMMPGLDGLATLQEIKRINPALPIVMVTKSEEEGLMDKAIASQISDYL
                     IKPINPNQIIMAIKKIFQADEIRANQIGQQYTRYIAELNQKLFATPDWDEWSQIYREL
                     SYWELQIDQVNDEGLRQTHFLEKRNCNTEFTNYVERNYNKWLKSDERPNLSFDLISQH
                     IAPHFEENLPIYFIIIDCMRLDQYLAIQPYIQELFDEELDLYYSVLPTATPYSRNSIF
                     SGLLPIDIAKRFPDYWVTSSEMDNSRNRNEHQLLDEHIEELGYKLDPTSKYVKIFNME
                     EGNFVLRKIETWNKENLIVLVYNFLDLLAHHRSRDQILQETIPNEEGLRAFTKHWFLH
                     SSLYEALKLIAKQDAIVILSTDHGSIKVNRATQVIGDKDTSVTVRYKEGKNLSVNDRH
                     ALFVKKPSDYGLPAKSIVDNFIFAKDDYYFVYPNAYHQYQRQYNGTFQHGGVSMEEMI
                     LPIATCRTKKRTKE"
     gene            complement(8272..8892)
                     /locus_tag="PKM80_00210"
     CDS             complement(8272..8892)
                     /locus_tag="PKM80_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92358.1"
                     /translation="MPNKIMPPESMSAEEKFAALANLKDKLEDNFVMLGQLLSEIKRS
                     KLYLFKGYENFKDFVETEYQLSGSLAGKLISTFDLFIEEMDIDENEVKEIGFDRLQMI
                     KPFMKKADWQVRDEWIHKAEEMPFQELRDHIKELKEKEKETNVDLKEVYIEQFMEKML
                     GWFNCSRKELNFKLALFFQDADLDQMKKIVKERQRLFELETQTNKE"
     gene            complement(8919..9743)
                     /gene="panB"
                     /locus_tag="PKM80_00215"
     CDS             complement(8919..9743)
                     /gene="panB"
                     /locus_tag="PKM80_00215"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNV92359.1"
                     /translation="MTKVKNKKNGIALFQDMKKCKEKISMITAYDYSMAKCVSATSID
                     IILIGDSLGMVVLGYEDTLQVTVENVIYHCSAVRRGAPETFIIADMPYLSYHLSEEQT
                     RKNAADLIIKGKADAVKLEGGYPSRIDAIKAIVDMEIPVCAHLGLTPQSIHKFGGYKV
                     QGKKPEQQEELLKQALAVEKAGAFMLVLEGIPEILGKEITEQINIPTIGIGAGRYTDG
                     QVLVYNDLLGYSDMQPKFVKHYAEINSNIKEAIELYSQEVKNGVFPAPEHIYYPIT"
     gene            complement(9724..10290)
                     /locus_tag="PKM80_00220"
     CDS             complement(9724..10290)
                     /locus_tag="PKM80_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92360.1"
                     /translation="MWLSVTNAIKLNFGKKIKANCISCSAKIIILSFFIFSLGPTIIF
                     AQSNSEEYLEVKNWKAPKDAVAFTELTVQDSIYLYKDKPFTGVAVEHYPNGNLLRAVT
                     YIRGKQSGPMFLWYPDGNPQMSAYYQNGYLHGRFLGWYSNGSVIYDIVINKGTYAGDN
                     LLDEDEGRIGLEMEEGEREGPDNDQSKE"
     gene            complement(10719..11459)
                     /locus_tag="PKM80_00225"
     CDS             complement(10719..11459)
                     /locus_tag="PKM80_00225"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNV92361.1"
                     /translation="MLLWRLLLALFISDFLLQNGRLIKAKKNFTGLIIHCAIYFVVML
                     CLTANLLTGKIIVVLLILAILHGIVDYAKQILRPIFANREWLLFITDQFIHILTIILA
                     VGIISDLDCSYLIAIYNKFPIDAWFKYLALFIVTVFAGIYFTDSALQGFLKHFTKPDN
                     TKQKPNAKQQEYASRWIGVSERFLITISVLIGRYELIGFLIAAKSLIRLPEIQDEKTE
                     DFSNYFLVGTLLSYSWAIAITLLFKKLF"
     gene            complement(11459..11644)
                     /locus_tag="PKM80_00230"
     CDS             complement(11459..11644)
                     /locus_tag="PKM80_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92362.1"
                     /translation="MANLSVIEHICLAVYVSTKYGLAGINKPNTVVSLKVNWNWLMLF
                     NVSFSLSPNKSSLLEGE"
     gene            complement(11674..12339)
                     /locus_tag="PKM80_00235"
     CDS             complement(11674..12339)
                     /locus_tag="PKM80_00235"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HNV92363.1"
                     /translation="MIAVLTCDIISSRSYDKKQRQILQTSISDAFSYTCKIIPEAKAD
                     YMSFSVIQGDEFQFNIEEAPYFYHFLFTFRNQLSLCGIEPPPFFRAGIGFGTRTIEGK
                     NNSYQMDGTAYHNSRFALNSFSENAYKKRLSIVRLDNPLLTKCYNTVLAFCDDWESSI
                     SFPQRRAIQQILTGLTQRQAAQNLGFTEQNMVKLLKRAKWDLFSQAMDFFRTTLPENL
                     PTQ"
     gene            12831..13292
                     /gene="ribE"
                     /locus_tag="PKM80_00240"
     CDS             12831..13292
                     /gene="ribE"
                     /locus_tag="PKM80_00240"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNV92364.1"
                     /translation="MKVIEGKLISRGYKFALVVSRFNEFISSRLLEGALDCLKRHNVN
                     DEDISVIWVPGAFEIPLIAKEAALKEDIDAVICLGAVIRGSTPHFEYISAEVTKGIAT
                     VGLETRKPIIYGVLTTDSIEQAIERAGTKAGNKGFTAASSALEMLNLIAEM"
     gene            13295..13762
                     /gene="nusB"
                     /locus_tag="PKM80_00245"
     CDS             13295..13762
                     /gene="nusB"
                     /locus_tag="PKM80_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNV92365.1"
                     /translation="MGQRRKAREMAVQCLYSLEFSEVDKEYREYGLLNEYPEILLSLA
                     KQEHIQPNSPVCAFAEELVKNSIINIEQIEAVLDKLTDNWDLEDIALLDRSILCISIY
                     ELLYTDTPAPVVINEAIEIAKKFSSESSGKFINGILDALNKEIIQNQKGNLPI"
     gene            13766..14674
                     /locus_tag="PKM80_00250"
     CDS             13766..14674
                     /locus_tag="PKM80_00250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV92366.1"
                     /translation="MNKIKCSVGIFAHNEAANIIPLLQAVENQELQEAEISEIIVVSS
                     ASTDGTDTLVSSYAKDHNKVTLFTQPKREGKSSAINLFLANAKEDIVVIISADVIPQK
                     KTIEKMVSAFKDPKIGATGGRPVPVNDENTYLGYVVHLLWRLHHRMALISPKLGEMIA
                     FRKVMKSIPKDSAVDEASIEAIIKEQGYKLKYIPSAIIKNKGSENLSDHIKQRRRIQN
                     GHLWLKAHQHYKVSSQDSGILLKVVLQELIERPGSFFKLALAMLIEIYCRLLGSWDYY
                     IKGKNPFAWEIARSTKELQRPIKDKE"
     gene            14677..15759
                     /locus_tag="PKM80_00255"
     CDS             14677..15759
                     /locus_tag="PKM80_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV92367.1"
                     /translation="MIYLIFRIMGYWLMKNLGFPHLMPMNYTVSLLYTCNSRCSTCNI
                     WKKQANNLSVDEYKKIFKHIKHTPYWITFSGGEPFLRNDIVEVVTSIYQISRPRIINI
                     PTNGLLVKTIIEKTDAIAKACPKAQIIINVSVDGIDEQHDNIRNVPGNYKKAIATFNG
                     LKALKHKNLSVGIHTVISRFNVDSFPSIANELMRLKPDSYITEIAEERVELDTIGTDI
                     TPSLVAYKSAIDYLIHRIKNTTYKGMSKVTMAFRIEYYNLVKRIMRDKRQIIPCYSGV
                     ASCQISPEGDVWNCCVKAKPLGNLREAKYNFRKIWFSPAAELERRSIHKRECWCPLAN
                     AAYTNMLLHLPTLIRVSWRSFIRWWC"
     gene            15761..17170
                     /locus_tag="PKM80_00260"
     CDS             15761..17170
                     /locus_tag="PKM80_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV92368.1"
                     /translation="MISLHSCEASPPRSVCEYKASRDGSFAWFLLTFHPEKDNVLEEI
                     VAEIKLEVINSKINAISRLEIEKWLKQFFSDLHWKLYGILRKTTLNEKGISLFFGVLY
                     DDELYFVQFGRIFCVLADHKKMKPVGKDWRNYQVQSLEKMQLLGYNEKDLIIKPQRVR
                     IENNQLFVVISGELAQKIFLTVTDPVSIETLIETYITEDNPLWLILGGSENLAKPKRR
                     KLSRLQVTSIILIFLAVLAAVYVMVGNRFLDQMVHKMRLSVKEEKPLRLEQIPTNLNI
                     NSENLKKYMDKLVNMPARNISFTVAWTTELPYQVSLSPSFTLSTIYLVSGNRLSAYDK
                     KSRQLLWTKILEDEILSTTVHQGLLMIYLANQQILGLNTDGIVLWQQYLPTESITGDR
                     FVPCIISNSDDPRLDRSIMVIPSKRGISILDPAKGETLSSITLKQDLDALSVYDSFAN
                     CFYAVVDNAVLCVELNIVN"
     gene            17174..>17414
                     /locus_tag="PKM80_00265"
     CDS             17174..>17414
                     /locus_tag="PKM80_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNV92369.1"
                     /translation="MHRYFIILGLVICLPLLAVVETPIKTGFARLQYDGGGDWYNDPE
                     VLPNLAKYANLVMGTSFPLDKSEVKASDPKLFDFPF"
BASE COUNT         5542 a         3027 c         3236 g         5609 t
ORIGIN      
        1 taaaggagag gactttttac ttttaccact tacagaaaaa agaaaaacct cttgacaata
       61 atggcaatgc aaaaaaggta aaaaacaaag tgcaggtaaa tgctaaaaat tgaagtggta
      121 acaactggat aaggagaaga aagtgagcat cttgttctca gaggaccaat tatgttaagg
      181 tatagtttat tattacacaa acagcataat taagttgata gtggaacggt aggatgtagt
      241 tctttcggat tctcgataga aaaatgccaa agaaaggaga taaccaaaag tatgttaaaa
      301 gtatcgattg ccccagatgg aaaagaatat ccgcttccca ttgaagaaga cgctgaaaaa
      361 gaacgcgaga ctttaaagcg tattacagct gaacaacgtt ccttgggtcg taaaatagtt
      421 gctgttcaag gtttaggttt tgtaggttgt gtaatggcta ctgtcgttgc tgatgctaca
      481 gataaagatg gaaacccgat ctattttgtc catggtcatc aacgtgcttc tcaacgttcc
      541 tattggaaag ttcctgttat caataatggg gttccaccca taaattcatc tgacccagaa
      601 gttcctgaaa tatttcatcg aaccgtagta gagaagaaaa atttccgtgc taccagtgaa
      661 gagagtgttt acagtttagt agatattgta gtagtagata ttcagttgga tgctacaaag
      721 cctgcttttg gagatgcgga aaaaggttat tgtgatataa ctgcattccg agaaggcatt
      781 cgcactttag gtaagtatat tcagccagaa tgtttagtgc ttgtagaaac gactgttcca
      841 cctggaacct gtgaacaagt agtcaaaccg attttggaag aagaatttac taagcgcgga
      901 attgatatca ataatcaacc accccttatt gctcattctt atgaaagggt gatgcctgga
      961 gcacattatg ttgaatccat tcgtaatttt tggcgtgtgt attcaggagt gaatactaaa
     1021 agcatagagc tttgtagaga gtttctttcc aatgtattag atgtggaaca ttttccatta
     1081 actcagttgg atagcacaaa tgccagtgaa ttagccaaaa caatggaaaa ttcctatcgc
     1141 gctgtgaata ttgctcttac tttggaatgg gcaaaatttg ctgaacagat tggcgtggat
     1201 atttttaaag tgcgcgaggc gattcgtaaa cgcaaaggaa cacatgataa tctgttaaga
     1261 ccatctttag gagttggcgg atattgttta acaaaagacc ctgtgctagc taattgggct
     1321 atgaattctc tttttggagt ggaaggaact ttagattttg ccataaaagc tgttaacact
     1381 aatgacacta tgcccttgca tactattgat atcgtaaaaa gcgtgtatcc cgaactaaaa
     1441 aatttgaaga ttgcagtttt aggcgtttcc tatttacaag atgttggcga tacgagacat
     1501 tcaccatcca agatcttagt ggaattttta cggaaagacc tggctattgt taaaactcac
     1561 gatccctatg tagaatcctg gcctgaaatg gaagaaaatc atatacagag cgatttgaat
     1621 aatgttttac ccgaatctga tgtagttatt tttgccgttg ggcacagtca atacagaaat
     1681 ttggatccag aacaactttt ggctatctgc aaaacgaaac ccttaatcgt agattgttct
     1741 aatttcttat ccgatgaaac tattaataaa tacaaagctt tgggctgtgc tgttagggga
     1801 gttggtaaag gacatataat agattagtga gataatgata taaaaagtgt agtcggagga
     1861 cgctgttctt ccgcaaaatg aaaaaatgca gagcttcagc gagctccgtc tacacaaagt
     1921 tgattcctaa ttcataaatt ggtgaaggac actttcgaga atgaaattct gtattccgtg
     1981 tcctgataat tctgcttggc tcatttattt tggtgatttt tgtacttttg gtggctaaaa
     2041 atatgggagg ataaatgatt ttaacagatg ctcaattatt tagtgaaata agtcgctgtg
     2101 aatattgtga aaataaacct tgtaaaactg cctgcccttg tgattgttct ccagcagatt
     2161 ttatgatggc tttagccaag ggaagacctt cggatttcaa aagagctgca gggataattt
     2221 tagccagcaa tcctttagga ggaatttgcg gaaatgtatg ccctgattat cattgcgtag
     2281 atgcttgttc acataaaaaa ttcgataagc cgatccaaat tcctgatgtg caagcaacta
     2341 taattcgcaa agcgcgtgaa ttggatttgg tgccagaatt ttcatctcca attcgtctaa
     2401 acacaaaaat tgctattgtc ggtgctggac cttctgcaat tggagctgct gttactttaa
     2461 atcaatttgg ctacgaaacg agaatgtttg atcctgaacc tttaggagga cagataaatt
     2521 tgatttcgga agagcgttta gaagaaattg atataatagc agacctgaat ttcgtagtaa
     2581 aaaccttccg attgcagcat attccaaaaa agatagaaaa acctgaagaa ctcttgaaag
     2641 aaggttatgc ggcagtaatt gtagcaagtg gattaaatgt accaattcgt ttaaacatcc
     2701 ctggtgatga aacagctatc tatggtttag atattctttc aaacccatca aaatataatt
     2761 tttctggtaa acgcatagct attgttggag gagccatagc tgccgatatt gcattagtgg
     2821 cagctaaaaa tagtgctgct tatgtagaaa tgattacgct ggagaatttt gtggaaatgc
     2881 caatcacaga tagggagaaa gcaactttaa ttgaggcagg aattgaattt tctcctcgca
     2941 cccggatatc agaaatagtt aatgaaaatg gtcacttaaa aggaataaag actaaaaaag
     3001 tctatctacc caagaatgaa aaattcgcac ccaatctgat taaagatgaa gctggagtaa
     3061 ttgataacta ccgcatattt gatatggtta ttatagctat tgggaataga gctactttta
     3121 atgttcacga gctggaaaga aagtttgata atattttttg ttgtggagat atggttaatg
     3181 gacccacaac cgttgttgaa gcagtagcct caggaaaaaa tactgctatt aaagtacact
     3241 tatctcttag taacaaagca atagaaaaac cggaaaagga aactaaaagt tgtttccctg
     3301 tttgggggtg gaaaaaatat cctgtttcct tagaaactga tttttttggt agaataatgg
     3361 attcgccttt tattttatct gcggctcctc caactgatgg ttttgagcaa atgcaaaaag
     3421 catatcaagc tggttggagt ggaggcataa tgaaaaccgc ttttgatgat caagatattc
     3481 atattccggg tgaatatatg ttcacttttg gcgataaaaa gaagaccttt gccaattgtg
     3541 ataatgtttc agagcattct ttaaacagag tttgcaaaga aatagctcgc ttaataaaag
     3601 aatttcctga tcgcttaaca atggcttcta ctggaggtcc tgttaccggt aatgattgtt
     3661 ctgataaaca atgctggcaa tccaatacta aaaaattaga acaagcagga gcaatgggaa
     3721 ttgaatttag tttatcctgt cctcagggtg gagatggaac taaaggtgat attgttgctc
     3781 aagatcctgt tttaactgca aaaatagtag attgggtaat ggaaataagt gctccggaaa
     3841 taccgaaact ttttaaactg accggtgcgg taacttctat ctatccaata ctttcagcca
     3901 tcaaaaatgt gttaaataaa tatcctcata aaaaggcagg tatcacttta gctaatactt
     3961 ttccagcttt agcttttcgc gaaaaagaaa aaccctggga tgaaggaata attgtaggaa
     4021 tgagtggtga aggtgtatta cccatcagtt atttaaattt agcccgcgct ggaaaccaag
     4081 gcatctttat ttctggaaac ggaggggcaa tgaactatct ggatgcagca aactttttag
     4141 ctttaggggc aagaaatgtt cagttttgta caattgtaga aaaatacggt tacaacataa
     4201 tagaagaact gaaaatggga ctttcctatc tgttggaagc gcgaggttta aaaagtgtgg
     4261 ctgaattggt aggaattgct cagcccaatc ctatcataga ttttatgaac ctgcctaaaa
     4321 aaataaagag ctcttctctg aacagggaat tatgcttaaa atgtggaaat tgcactcgct
     4381 gtccctatct ggcaatagaa cttgatgagg attgttttcc aaaaattgat aacgatcaat
     4441 gtgttggatg ctcactatgc actcaattat gttttaccgg agccttgcaa atgaaaaaaa
     4501 tatagagcgc taaaaagtgt gataggtata aggtcaatta tgagcttatt gagctttcag
     4561 ggtttcagta tagaccccta ctttatatat tcgtttataa ttctttgaag ttcaccgctg
     4621 tcttttaatt ctttcagtgc tttattgatt cttaaattaa tttcttttcc aggtcgcata
     4681 gccagtccgt actgttcatt ggttgggatg atatatacta tttttatatc tacttgttcg
     4741 gaaacctttt tggcaaccca ttcatcaaag accatcaggt ctattttctt attgagtaat
     4801 tctcctatgg tttcaatcaa ggtagagtat gttttcaatt tctccttagg taggaggtct
     4861 ttatcgacca ggttttcttt taaatattgg tagctggtag tgctgctttg acagccaact
     4921 ctatattttc caatatcttc aattttattt atttcaattg gagaggcttt tcttgctact
     4981 acagctaaat tagtttgaaa atagggttct gagaattcaa atgtctgttt tcttttttct
     5041 gtaattgtta tggtagatgc ggcaagatca attttgtttt gagctaatgc agagaataag
     5101 tcatcaaatt catactgggt aatttgcagt tgcatcccca ttttatctgc tattttttca
     5161 catatatcga tatctatccc tttaaattca ctattttcca gataagtaaa gggaggattt
     5221 tcagcatctg tgcccactct gaggattttt ttctgctcag gagaacagga cgcaactaac
     5281 aatattattg ttagcaaaag agttagaaca aaaaggttct tattcatttc cacttgcttc
     5341 gatacaggat cttttaaata taatctaaac gatccccttt ggctaacaag taaattagtc
     5401 agaatattgt attttgcttg tggaaatatt tctactttag cttaaagata gcggtaagag
     5461 taacggaaat gcttttactt cttgtacttg tattataaaa tccataatcc gatacttctg
     5521 ttgaataggg ttcagtaatt tgaaaaactc cagctcgtgc ttctctcaat tttcctaatt
     5581 tagtatttcc gcttcccgaa atttcttcag ctcgagctac tgcatcttta gttgcttcag
     5641 atagcaattg ttttttcaaa tctggcagct tagtatagag ataagcaaga ttagaatttt
     5701 gcaagagtat tccgcgttca gcaaagaaat ctggattcaa ggagagtttt tcaattttgt
     5761 ctatatctgt agaaaccaca aagatacgct gatttaagca ataaccagta gggttgccat
     5821 agttatcgtt tgtttgatag ctatttgtag gttgaatgct gatatctttt tctggtattc
     5881 cttgttccaa taagtacttt ttaaaggcat taacatcggc actcattttg ttgtagccat
     5941 cctttaagcc atcgttacta acattacgct gcattgttag attccattta acgatgtccg
     6001 attcaaagag tttactggca taacccacaa cgcgtaaact tgttggtgct tgacggcttt
     6061 gcataaaaaa atagccaaat actgttaatc caataacaaa agaaagacca acaatacccc
     6121 aggtgcggga tattgcttta tctttagcaa agataatcca acccaggcat aaagcaattg
     6181 caatacagat taatactaat agtacagtaa acattttttt tctccttatt caaagtactg
     6241 tttatgatcg tcgtctggaa atacattcac ccacctcgat tttccgtcga agtggaaatc
     6301 aagtttgaaa gtttgatagg gtaagatact ttcagctata tgtggccatt caataacggt
     6361 tacccctttt tccaaaatat caaatatccc taagtcgtaa agttcttcct tatcttttaa
     6421 acggtataga tcaagatggt aaagaggaag gcgtccacct aagtattctt tcattataat
     6481 aaaagaagga ctatcaattt cttcattgat tcccagttcc ttacccaagt gtttaacaaa
     6541 aaaggtctta ccgcttccaa gatctccata aagggtaatt atatctcccg ctttcaagat
     6601 tggagcaaga taattggcaa gatctatagt atcttgttct gaaggtagat ttattgttat
     6661 catactattt tatttaactt gctttcttac cttttttcat tcttttgttc tttttttagt
     6721 cctgcaggta gcaataggca gaatcatttc ttccatagaa actcctccat gttggaaagt
     6781 gccattatac tgtctctgat attgatgata agcattagga tacacgaaat agtaatcgtc
     6841 tttggcaaag ataaagttat ccacaatgct ttttgctggg agcccataat cactgggttt
     6901 ttttacgaat aaagcatgac ggtcatttac ggaaaggttt ttcccttctt tataacgaac
     6961 agttacagaa gtatctttat ctccgataac ttgagtggca cggttaactt ttatcgagcc
     7021 atgatcggta gaaagaatta caatagcatc ctgtttggca atcagtttta aagcttcata
     7081 tagggaagaa tgcaaaaacc agtgtttagt aaaagcacgc aaaccttctt cattggggat
     7141 agtttcttgt aaaatttgat cccggctacg atgatgagcc aaaagatcaa ggaagttata
     7201 taccaaaaca attaggtttt ctttgttcca ggtttctatt ttacgcagca cgaagttgcc
     7261 ttcttccata ttgaatattt ttacatactt gctggttgga tcaagtttat aacccagttc
     7321 ttcaatatgt tcatctaaaa gctgatgttc attacggttt cggctgttat ccatttcact
     7381 ggaagttacc caataatcgg gaaagcgttt agcaatatca atcggcaata aaccactgaa
     7441 aatggaattt ctgctatagg gtgtagctgt aggtaaaaca gaataataga gatccaattc
     7501 ttcatcaaaa agttcttgga tatagggttg aatagccaaa tattgatcca ggcgcataca
     7561 atctataatt ataaaataga ttgggagatt ttcttcaaaa tgcggagcta tgtgttgaga
     7621 aatcaaatca aaacttaagt taggccgttc atcacttttc agccacttat tataattgcg
     7681 ttccacatag ttagtaaatt cagtattgca atttcgcttt tccaaaaagt gagtttgacg
     7741 cagaccttca tcgtttacct gatctatttg caattcccaa tagcttaatt cacgataaat
     7801 ttggctccat tcatcccaat cgggagtagc aaaaagtttt tgattcaatt ctgcaatata
     7861 acgcgtatat tgttggccaa tctgattagc tcttatttca tctgcctgaa agatcttctt
     7921 aatagccata ataatctgat taggatttat gggtttaatt aaatagtccg aaatctgaga
     7981 ggcaatagcc ttatccatca aaccttcttc ttcacttttt gtaaccatta ctataggtaa
     8041 agcaggattg attcttttaa tttcttgaag ggtagcaaga ccatctaaac cgggcatcat
     8101 ttcatccaga attaccagat cgaatttatt ttctaccact ttttcgatag catcagttcc
     8161 gttattacag gtatcaacca gatagtttct ttcctccaaa aagaggataa agggtttcag
     8221 taggtctatt tcatcatcca cccaaagaat atgcatttgt ttcattggat tttactcctt
     8281 gttagtttgt gtttcaagtt caaagagacg ttggcgctct ttaacaatct ttttcatctg
     8341 atccagatca gcatcttgaa aaaatagggc aagcttaaaa ttaagctctt tacgagaaca
     8401 attgaaccat ccaagcatct tctccataaa ttgctcaata tatacctctt ttaggtctac
     8461 attggtttct ttttctttct cttttagttc ttttatatga tcacgaagtt cttgaaaagg
     8521 catttcttcc gctttatgaa tccattcatc acgaacctgc caatcggctt ttttcataaa
     8581 gggtttaatc atctgtaaac gatcaaagcc aatttctttc acttcgtttt cgtcaatatc
     8641 catttcttca ataaacagat cgaaagtaga aataagtttt cctgctaagc ttccggagag
     8701 ttgatattcc gtttctacaa aatccttaaa attttcatat cctttaaata ggtacagttt
     8761 agaccgtttt atttcggaaa gtagctgacc gagcattaca aaattatctt ccagtttatc
     8821 tttcagatta gctaaagccg caaatttttc ttcggcgctc attgattcag ggggcattat
     8881 tttgttaggc atctttttta tccttctttt tacagcattt aagttatagg gtaataaata
     8941 tgttcaggag cagggaaaac cccatttttc acttcctgag aatagagttc aatcgcttct
     9001 ttaatatttg aatttatttc agcataatgt tttacgaatt tgggttgcat atctgaatag
     9061 cccagcagat cattataaac caaaacttga ccatcagtat atcttccagc accaattcca
     9121 atcgtaggaa tgtttatttg ttcagtaatt tctttaccta aaatttccgg tataccttcc
     9181 aaaacaagca taaaggcacc tgctttttcc actgctaaag cttgttttaa aagttcttcc
     9241 tgttgttccg gttttttacc ttgaacttta taaccaccaa atttatgaat ggattggggg
     9301 gttaaaccta aatgcgcaca cacaggtatt tccatatcca caatagcttt tatagcatct
     9361 atacgagaag gatagccacc ttccagtttt acggcatccg cctttccttt aattataagg
     9421 tcggcagcat ttttccgggt ttgctcttct gaaagatgat agcttagata gggcatatca
     9481 gcaataataa aagtttccgg agctcctcta cgaaccgctg aacaatgata aattacattt
     9541 tctacagtca cttgcagagt atcctcatag cccagaacaa ccattcccaa agaatcacca
     9601 atgaggataa tatctataga tgttgcagac acacattttg ccatagaata gtcgtatgca
     9661 gtaatcatac tgatcttttc tttgcacttt ttcatatctt ggaaaagagc aattccattc
     9721 tttttattct ttactttggt cattatcggg tccttctctt tctccttctt ccatttctaa
     9781 acctatccgg ccctcatctt cgtccaataa gttatcgcca gcataagttc ctttattaat
     9841 tactatatca taaataacgc taccattgct gtaccagcct aaaaagcgtc cgtgtaaata
     9901 tccattttgg taatatgcac tcatttgagg attaccatca ggataccaaa gaaacatagg
     9961 cccactttgc tttcctctaa tataggttac agctctaagc aaattaccgt taggataatg
    10021 ttcaacggca actccggtaa agggtttatc tttgtataaa taaatactat cctgcaccgt
    10081 aagttctgta aaagcaaccg catctttagg cgctttccag ttttttacct ctaaatattc
    10141 ctcgctatta ctttgggcaa agataatcgt agggccaagg gaaaagataa aaaaggatag
    10201 tataattatt ttcgctgaac agcttataca gttagcttta atctttttac cgaaattcaa
    10261 ttttatagca ttagtcactg aaagccacat ttttttactc caaagggtta gtcaatcttt
    10321 ttttatttat tgcgagaaac cccgttcatt agatatgata atctaaactt tgtcttttca
    10381 aaacaaagaa ttatcaagcc ataataaagc taaatttata taaggtcggg gaacagcatt
    10441 acctaatagt aaggccataa ctatctctat ctatgtataa tcctttttcc tccattgaat
    10501 ttatctcgag ttatgctcta agaaaagaaa tgaatctcct cttcaattaa taaaataaac
    10561 ctagtttttg cttagtcacc tgtggtagaa ctgcggtgga aatgctcggc aaattcctct
    10621 gtaacaccat tccagcacta ccgtagataa ttaagcaaga acaaacctgc caataattat
    10681 ttaaaaggat taaaacagtt tccctatagg attgtttgtt aaaataactt cttgaataac
    10741 agcgtgatag ctattgccca agaatagctt agtagcgtgc caaccagaaa ataattactg
    10801 aaatcctcag ttttttcatc ttgaatttca ggaagccgaa ttaaggactt agcagcaatc
    10861 aaaaaaccaa ttaattcata acgaccaatc aatacagaga tagtaatgag aaacctttct
    10921 gataccccaa tccaacgcga cgcatattct tgttgttttg catttggttt ctgctttgta
    10981 ttatcgggtt tggtaaaatg ttttaaaaaa ccttgcaggg cgctatccgt gaagtaaata
    11041 ccggcaaaaa cagtaacaat gaataaagct aaatatttga accaggcatc aataggaaat
    11101 ttattataaa tggctattag ataactgcaa tctaaatcgg aaattatccc caccgctaaa
    11161 atgatggtta atatatgaat aaattgatca gttatgaaca gtagccattc tctattggca
    11221 aaaatagggc gtaatatctg tttcgcatag tccacaatcc cgtggagaat ggctaaaatc
    11281 aacaaaacta caatgatttt acctgttaac aaattggctg tcaggcataa cataacgaca
    11341 aagtagattg cacagtgaat aattaaaccc gtaaaattct ttttcgcttt aatcaaccga
    11401 ccattttgca gtaaaaaatc actaataaac aaagctaaca ataaacgcca taatagcatt
    11461 attcaccttc cagtaatgaa cttttattag gagataagga aaagctgaca ttgaataaca
    11521 tcaaccagtt ccaattaact ttcaaactta caactgtatt tggtttattt atgcctgcta
    11581 aaccatattt ggttgagaca taaacagcta aacaaatatg ctctattact gataaatttg
    11641 ccattttcat tgtgcttata gtattttcca acatcactga gtcggtaaat tttcaggaag
    11701 agttgtgcga aaaaaatcca tcgcctgaga aaacaaatcc cactttgcgc gttttaaaag
    11761 tttaaccata ttctgttctg taaatcccaa attttgagct gcctggcgtt gagtaagacc
    11821 tgtaaggatt tgttgaattg ctctgcgttg tggaaaacta atggaacttt cccaatcatc
    11881 acaaaaggca agaacggtgt tataacattt agttagtaga gggttatcta accttacaat
    11941 gctcaacctc tttttataag cgttttcaga aaagctattt agagcaaaac gggaattatg
    12001 atatgcagtt ccatccattt gatagctgtt gtttttccct tcaatggttc tggttccaaa
    12061 accgattcca gctcggaaaa aaggaggtgg ttcaattcca cataaagata gttgatttct
    12121 aaaagtaaac aaaaaatgat agaaataggg cgcttcttca atattgaatt gaaattcatc
    12181 cccctgaata actgaaaatg acatatagtc agccttagct tctggaatga ttttgcaggt
    12241 ataagagaaa gcatcagaaa tactggtctg taatatttgt cgttgctttt tatcgtaaga
    12301 tcgagagctg ataatatcac aagttagaac agctatcatt atttcctcaa atcgattttt
    12361 atgttattaa cattatatta ttggatttga taagtgtcaa gcaaaaagct gaagcaagat
    12421 ggaattttct ttttagttcc cgaggattca atttttcctt tggttgaatg ctactggtta
    12481 tgaaaaagtg atttatacag aaaatatagg tagcgccgaa atatgaaaga ttttccttga
    12541 acaaattcca ataatttaaa taatggtatt tagcataaaa atagacaaga taacaggagt
    12601 taatatagca aagtgggaaa acttcacttc tctaagtgct aatatagcaa aagttttcta
    12661 tcctttgaat aatatatacc ctattattat agtgagccga tatattacct ttgggagtac
    12721 aaaagaggta taaatattag agatattacc gcccctaaag ataaggaaga aaatattcta
    12781 cagaatgcgg agtttatgga aagctccgga tacgcaagga gaaaaaaaag atgaaagtta
    12841 ttgaaggaaa attaatctcc agaggatata aattcgctct ggtagtcagt cgttttaatg
    12901 aattcatcag ttctcgttta ctggaaggag cattggactg tttgaagcgt cataatgtaa
    12961 acgatgaaga tattagtgta atatgggttc cgggagcttt tgaaatacct ttaattgcta
    13021 aagaagcagc tttaaaagaa gatatagatg cggtaatctg tttaggagcc gtaattagag
    13081 gttccactcc ccattttgaa tatatcagtg cagaagttac gaaaggcata gctactgtag
    13141 ggttagaaac ccgtaaacct attatttatg gggtgttaac tacagatagt attgagcagg
    13201 caattgagcg tgctggaact aaagctggta ataaaggttt tacagccgcc agttctgctt
    13261 tggaaatgct aaatcttata gctgaaatgt aaaaatgggg caacgtcgta aagctcgtga
    13321 aatggcagtt caatgtcttt attctctgga attttcagaa gtggataaag aatatcgtga
    13381 atatggtctg ctaaatgaat atcccgaaat tttgctttcc ttggcaaagc aagaacatat
    13441 tcaacccaat tctcctgttt gtgcttttgc tgaggagctg gtaaaaaact ccatcatcaa
    13501 tattgaacaa atagaagcag tactcgataa actaaccgat aattgggatt tagaagatat
    13561 tgccttgctg gatagaagta ttttatgcat ttctatatat gaactattat ataccgatac
    13621 acctgctccg gttgttatta atgaagcgat tgaaattgcc aaaaaattca gcagtgaatc
    13681 ctcaggcaaa tttattaacg gcattctgga cgcccttaat aaagaaatta ttcagaacca
    13741 gaaagggaat ttaccaattt aagctatgaa taagatcaaa tgctccgtgg gaatttttgc
    13801 tcataatgaa gcagctaata tcattcctct gttacaagca gtagaaaatc aggaattgca
    13861 ggaagcagaa attagcgaaa taatagttgt ttccagtgcc agtactgatg gaactgacac
    13921 tttggttagt tcttatgcca aagaccataa taaagtaact ctcttcactc agccaaagcg
    13981 cgagggtaaa tcttccgcta ttaatctttt tttggctaat gccaaagaag atattgtggt
    14041 aatcatcagc gctgatgtta ttccacagaa gaagacgata gaaaaaatgg tatctgcctt
    14101 taaagatcca aaaattgggg ctacaggagg caggcctgtt ccagtaaacg atgaaaacac
    14161 ttatcttggt tatgttgttc atcttttatg gcgtttacat catcggatgg ctttgatatc
    14221 tcctaaattg ggagaaatga tcgctttccg aaaggtgatg aaaagcatcc ccaaagacag
    14281 tgctgttgat gaagccagca ttgaagccat catcaaagaa caagggtata aacttaaata
    14341 tattccttcc gctattatta agaataaggg atcagagaat ttatctgacc atataaagca
    14401 acgaagaaga attcaaaatg gtcatttatg gttaaaggcg catcagcatt acaaggtttc
    14461 ttctcaggat agtggaatcc ttctgaaagt agtgttacag gaattgatag agcggcctgg
    14521 aagttttttt aagcttgctt tagctatgct tatagaaata tattgtcgtc ttctgggcag
    14581 ctgggattat tatataaagg gtaaaaatcc ttttgcttgg gaaattgccc gttctacaaa
    14641 agaattgcaa agaccaatta aggataaaga ataaatatga tttatctcat ctttagaatt
    14701 atgggatatt ggttgatgaa aaacttgggt tttcctcatc tgatgccaat gaattatacc
    14761 gttagtttac tgtatacatg taactctcgt tgcagcactt gtaatatctg gaaaaaacag
    14821 gcaaataatc ttagtgttga tgaatataag aagatcttta aacatattaa gcatacccct
    14881 tactggatta cttttagcgg aggtgaacct tttctgcgta atgatattgt agaagtggtt
    14941 acctctatct atcaaatttc tcgtccccga atcataaata ttccaactaa cggtttgctg
    15001 gttaaaacta taatcgaaaa aactgatgct attgctaagg cttgtcctaa agctcagatc
    15061 ataattaatg tatctgtcga tggaattgat gagcagcatg ataacattcg taatgttcct
    15121 ggaaactata aaaaagctat cgccacattt aatggtttaa aggcgctgaa acataagaac
    15181 ctcagtgttg ggattcatac tgtcatttcc cgatttaatg tagatagctt tccttccata
    15241 gccaatgaac tgatgcgttt gaaaccagat agctatatta ctgaaattgc agaagaaaga
    15301 gtggaacttg atacaatagg aaccgatatt acaccttctc tggttgccta taaaagtgct
    15361 attgattatc ttatccatag aattaagaat accacttata aaggaatgag caaggtaaca
    15421 atggctttcc gaattgaata ttacaacctg gtgaaaagaa ttatgaggga taagagacaa
    15481 attattccct gttattcagg agttgcctct tgccagatat ctcccgaggg cgatgtttgg
    15541 aattgttgcg ttaaagctaa gccattggga aacttgcgtg aagctaaata taattttcgt
    15601 aaaatctggt tttctcctgc tgcagaatta gaacgacgct caattcataa aagagaatgc
    15661 tggtgccctt tagctaatgc tgcttacaca aatatgcttc ttcatcttcc aactctaatt
    15721 cgcgtttcct ggcgtagttt tattcgctgg tggtgttgaa atgatatctt tacatagctg
    15781 cgaagcatct cctccccgaa gtgtatgtga atataaagca tcaagggacg gaagttttgc
    15841 ctggtttttg ttaaccttcc atccggaaaa agataatgtg ctggaagaaa ttgttgccga
    15901 aattaagctg gaggtgatta attctaaaat taacgctatc tctcggttgg aaattgaaaa
    15961 gtggctaaaa caattttttt cagatctgca ttggaaacta tatggaattt tacgcaaaac
    16021 gacactgaac gaaaaaggta tttctctctt ttttggtgtg ctctatgatg atgagcttta
    16081 ttttgtccaa tttggcagga ttttttgcgt tttagctgat cacaaaaaga tgaaacctgt
    16141 gggtaaggac tggagaaatt accaagttca gtccttggag aaaatgcagc tgcttggtta
    16201 taatgaaaaa gaccttatta tcaaacccca aagagtccgt atcgaaaata atcaactttt
    16261 tgtagttatt tccggtgaac tggcacaaaa gatttttctc actgttacag atccagtatc
    16321 tatagaaact ttaatagaaa catatatcac agaagataat cccctctggt tgattttggg
    16381 gggtagcgaa aatttggcaa agcctaaacg cagaaaactt tctcgcttgc aggtaaccag
    16441 cattatctta atttttttag ctgtactggc tgctgtgtat gttatggtag gcaatcgttt
    16501 tcttgatcag atggtacata aaatgcgcct ttctgtaaaa gaggaaaagc cactacggtt
    16561 agaacaaatt cccactaacc tgaatatcaa tagtgaaaat ttgaagaaat atatggataa
    16621 attggtgaat atgccagcca gaaatatttc ttttactgtt gcttggacta cggaactgcc
    16681 ttatcaagtg agtttatctc cttcttttac acttagcact atttatcttg tttccggaaa
    16741 tcgcctcagt gcttatgata aaaaatctcg tcaactgctc tggacaaaaa tactggaaga
    16801 tgaaatcctc tccactacag ttcatcaggg attgttgatg atttatctgg caaatcaaca
    16861 aattttgggg cttaatacgg atggaattgt tctctggcaa caatatctgc caacagaaag
    16921 tatcaccgga gatcgttttg ttccttgtat aattagcaat agtgatgatc cccgtctgga
    16981 tagaagcatt atggttattc cttcgaaaag aggaatcagc attcttgatc cagccaaggg
    17041 tgaaacacta agtagtatta ctttaaaaca ggatttagat gctctatccg tttatgatag
    17101 ctttgctaat tgtttttatg ctgttgtaga caatgctgtt ttgtgtgtag agctaaacat
    17161 tgtgaattaa gctatgcatc gctattttat tattctggga ttagtaattt gcctacctct
    17221 tttagcagta gtagaaactc ctataaaaac aggttttgcc cgtttacaat atgatggagg
    17281 aggcgattgg tataacgatc cggaagtttt acctaatctg gcaaaatatg ccaatttggt
    17341 tatggggaca agttttcctc tcgataaaag cgaagtgaaa gcatccgatc ctaagctttt
    17401 tgattttcct tttt
//
LOCUS       DAORHB010000004        16783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_2520_length_16783_cov_4.603539, whole genome shotgun sequence.
ACCESSION   DAORHB010000004 DAORHB010000000
VERSION     DAORHB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 16783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16783
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2520_length_16783_cov_4.603539"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKM80_00270"
     CDS             complement(<1..266)
                     /locus_tag="PKM80_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92370.1"
                     /translation="MKRILILISILACAVCQVEAISIYEVQFTSSRGSDGTFPSPYMG
                     KDVTLEGIVTATDYREGGFFISEKLNGPWRGILILDRNAKVQRG"
     gene            complement(530..2515)
                     /locus_tag="PKM80_00275"
     CDS             complement(530..2515)
                     /locus_tag="PKM80_00275"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNV92371.1"
                     /translation="MWKFFMNISLLVLLLCPVYIMGNYPKLSCYYLDCFMSDQNSQLV
                     SALSKYDLVILDMEMSEINPTALLSIRQLNPNVKLLAYMTCQEISTNIPNPIYQPLRY
                     SLRNSIQDEWWLRTATGEHTSFWQGTWMLNCTPFCPEVNGFTWIEHFADFINNNVLNN
                     PLWDGFFLDNCWSIVPYEIANIDCNNDGIVDNRDWLDSQWILGMEAMLNQFRIQNPTK
                     ILIGNGGYEYGQYLNGVMFEGWDYNNPNFEFGYDWSTFMNMYNNIEDTFVPPVMNIIQ
                     AMEPNGFLADISHFRFTLTTALMGSAYYAIDLGPSDHSDTWWFDEYDSDLGEPLEGFV
                     NPQISDQPNLLINGEMNSNLAGWELAFVSPTTGYLQNGFENNNSFAICNVTYADGNDY
                     SAQFMQYNNPNMDFQYGNRYLVTFKAKSAQPRTINFVISKHLANWEWVIPHSIPIELN
                     TDWTDYIFSVTSQNTSLNPDQLRFSFNLGNEPGIVYFDDIGLRLMEEELLMREFENGL
                     VICNPYNITLTAELTHPWYHLNGSQDHIVNNGQICTSVSIPAYDGIILRRTPLAIFDS
                     SENIPQVHMSFYPNPAKDKITLYLEATIKCSGRFCLYNLKGQKLRTEILPIIDTGKLE
                     VPLSLVDNNNRPLSKGIYIGSVKFGEFTRNFKLLVLY"
     gene            2802..5729
                     /gene="uvrA"
                     /locus_tag="PKM80_00280"
     CDS             2802..5729
                     /gene="uvrA"
                     /locus_tag="PKM80_00280"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNV92372.1"
                     /translation="MKTAIIIKGAQEHNLKNIDLTIPRNTLVVFTGVSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGQMEKPKVDYIEGLSPAISIEQKTTSKNPRSTVGTV
                     TEIYDYLRVLFARIGKQYCYNCGRPVGSQTIDQMIEHLMQNKTGTKLQILAPLVQNRK
                     GEHKDELEMLRQEGYVRVIINGQLRNLEEDIILDKKSKHNIDVVVDRIVIKEGIESRL
                     SDSLELALKLADGIIKIDYPEEKQEVILSAQNACPHCHIGFEELSPQSFSFNSPIGAC
                     TLCGGLGYKLEFDPDLVISDPGLSIMEGAVAPWGRLEAKQHSWTMNTVKNLARAFDFS
                     LSTSWKNLPEIAKQLILYGSKGKKFKNSWQNENGRGEFLMRFEGVIPQLERRMNETTS
                     EEMRRYYLQYIGDKPCPACNGNKLKPASLAVKIAGKNISDITAMSISEAYDFFAGLHL
                     KGNELLIAEEVLKEIKNRLSFLKNVGLHYLTLDRRSPTLSGGEAQRIRLASQIGSQLV
                     GVMYILDEPSIGLHQRDISKLVSMLLQLRDLGNTVIVVEHDEYTIRTADQIVDFGPKA
                     GIFGGEIVASGDLETIMHHPKSLTGAYLSGSIKIPTPEKRIKPDARKLSIINARHNNL
                     KNLNVDIPLGLFVCVTGVSGSGKSSLVNQTLSPYLHNHFYHTSHKVGKMDEIRGIEYI
                     DKIITIDQQPIGRTPRSNPSTYVKLFDPIRQLFAQLPASKIRGYKPGRFSFNVKGGRC
                     EACEGAGVKQIEMHFMADIFVTCEVCKGKRYNQETLSIRYKGKNIAEVLDMDVQEAIE
                     FFDAIPAIKSKLKTLQQVGLDYLKLGQPSPTLSGGEAQRIKLSRELSKVSTGNTLYVL
                     DEPTTGLHFDDINKLLKVLKKLVAMGNTVIVIEHNMDVIKSSDWIIDLGPEGGDEGGK
                     IICCGTPEDLINCPNSATGIHLQYHMQKEKELSSNSLSELETEMRPRKVSTKRKTDKS
                     QAL"
     gene            5726..7885
                     /locus_tag="PKM80_00285"
     CDS             5726..7885
                     /locus_tag="PKM80_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNV92373.1"
                     /translation="MSYQRFSYAPPTAGKHSVGIAGDFSSWEIISMEEKGGVHYLALE
                     LPPGVYQYKFVVDGSWILDENNPKKVPDGFGGYNSLLVVEPVTEVITWEDIMAELANK
                     TPEKFYQFFRSKENSYELRFFWYPELSDAIYLLYNNQQIQLAHIGQTPLYEVFYCLFE
                     HCGIFTFRLKIQYQNMFLFFGAEGFSEAEEDITPIEIELKAISIFSIPDWVGHSIIYQ
                     IFPDRFYNGNKDNDPDFREWYYEDCRTPPSSSKILSPEKEYYHMVDDWYDINGLKQSP
                     WQKKGVPDFYSFYGGDIAGVKQKLDYLLDLGINVIYFNPLWQAKSNHKYDSADYHSID
                     PHFATTQEMIDFVKLAHEKGIRIILDVAFNHTGETFWAFRDCVEKGPKSPYWNWYDWK
                     KWPLPKPLPPDFNPKDYYQCWWGIKDMPDLNYDLALPHPEENAVRNIKEAHPNAALVD
                     YLMSTVRWWLIDIGIDGFRLDVPDEVPFWFWELFRKEVKKAKPDAWIVGEIWYEARQW
                     VSPLYFDSVMNYAFFKNPVIEFFILSLLNKEEFCRKIESGLALYPFHSLKAMMNLLGS
                     HDTPRIFEISKGDVKRLKLAIAFQMCFIGAPHIYYGDEIAMEGGKDPDNRRPFNWNWE
                     NNPVAGDLRNFYKELISIRKAHPTLCEGHFAFIPAPDKIIAFQRELKGEKILVFLNTE
                     AESIAIDIPESSILLLGKIAERNYILPHSALILSELK"
     gene            8069..10213
                     /gene="asnB"
                     /locus_tag="PKM80_00290"
     CDS             8069..10213
                     /gene="asnB"
                     /locus_tag="PKM80_00290"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNV92374.1"
                     /translation="MCGIAGIYNFSKETVILTEVTGKKGILNTEPAGLSNDIDNLVKI
                     EGGYRQNGAKAILLKKMTEAIKHRGPDDEGYLIKDKNGIRSYSGMDSTEEIKSLYPLF
                     NSEITTLDNLSKKAVGKTVGAENIILGMGFRRLSILELKPIGHQPMYDKELGIAICYN
                     GEIYNYLELREELKKKGYKFCSQSDTEVIIKAYHFWGENCVQHFNGMWSFSLWDEKKN
                     LLLCSRDRYGIKPFYYAVQNGVLYWGSEIKQLMLTPIDKTLNQAMIWRSMKINSMLVY
                     DDETYWQNIHCLKPGHNLIVTNSKVIVKQYYDLDITSFETSQLSFNEAVEQYRNIFLD
                     SIALQLRSDVEIGATLSGGMDSSAIVCSAVHQKKEPLKTFSSYYAFPPELDERKWIEK
                     IIKETSCASYLISPKANDAIDLWEKLTFLNDLPLAAGFVSQNAVLQEAHKQGIKVILS
                     GQGSDELSAGYRHSLYRYFADLIRGVHISRLGKELPGYLKKDNRAFSKLSKIVLSTFL
                     PESSLYNLEFCYYRFEPFTRSFTNEAEISCGEQILKKIVDIKASRLSNFLYNMVHTTS
                     LQTLLHFEDRMAMANSIESRVPFLDYRLVDFVFSLPSQYKVKPPYTKVIHRAAMKELI
                     PEEIYQRTDKGIFSSPFYQSWMKQELKPYISDIFASSTFRQRGLWNLPKINHFWHKYL
                     GGDNSQVEMLYNVVALEIWFQQYENQSTGGLK"
     gene            10210..11094
                     /locus_tag="PKM80_00295"
     CDS             10210..11094
                     /locus_tag="PKM80_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92375.1"
                     /translation="MKNIVKIIALCFVLISLSSCKLFKSPTVTKIQDVKVVSITPDKS
                     TIKISLMVNNPNCYKLKLDKLEVELLNKDRDKVGYANLEKVVEIPKKAAMCINFNVEL
                     ETRPVVRMISSLNQKVQFFIVGKGHGKVKGLSKKFYFDEPYELDLKEELKNIIPKFSV
                     GTQNLFKITKTSVEKMSIGQTELHIHFIILNPYGLTYNLKGFPAEIYIDDKLAGTGNL
                     KNQLKFNENIFSQDGIMVFQLNNLKSIFGAVKGAIKGEIKYSVKGNVIIDAFGMQIKQ
                     PYSYQDSLPVNIVELILN"
     gene            11249..12844
                     /locus_tag="PKM80_00300"
     CDS             11249..12844
                     /locus_tag="PKM80_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92376.1"
                     /translation="MKKYLFLVLIILPVFLLGQVSSEETNIPGDSMPEEESEYPEGTQ
                     WGMGGAVGTVTIDNRTYSQIRLQPELQIGKFGIGLDIDLIIDAEGNVRKEDWDELQDY
                     VNKFLYIRFAQRNDPFYFKIGSIPSYTLGNGLIFNNYSNMILYPSVKNVGGYIGVNTN
                     ISGLGFEAYSHNIRKNEILAGRVHIQPLAATNIPLLEKLRLGVNAGIDRDQNAKYEDK
                     DGDHIPDIYDKFPKDKMRYLDTDNDGIADEDDIDIDGDGILDHPDYNPYIAQNFPGIE
                     DIAQGYNLDIVIFPDTAAAYNKKDEILIYSADYQLPLIETDHFNLVNYGELAKIDGYG
                     TGMIFPGFSSKFFIFDAKVEFRNFGDQFIPGYFDKQYDQQRAEVKHIVLEESGSQYWF
                     LTTKESNLENVEASLGWFGYLRANLFDFIYPKIAYQDMYGKNNFLGKSLWGSITINPY
                     MIPKLKEATIYYSQTNTNYINFRYLRNENAYISGRLVYSLSDNANLIGKYSEFYTDIN
                     CDGKIKGNDEVIESFTFGVEFQF"
     gene            complement(12848..14014)
                     /gene="manA"
                     /locus_tag="PKM80_00305"
     CDS             complement(12848..14014)
                     /gene="manA"
                     /locus_tag="PKM80_00305"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HNV92377.1"
                     /translation="MPYLIKPSLKDYSWGDIKYIQELIGALDMTGKPLAEAWFGAHPQ
                     SPSLIITEEGEIPMDKLLETYPESMLGKNQGNNLPFLFKILAAAQPLSLQVHPDRQTA
                     QYGFQKEEEARIPITDPNRIFKDPNPKPELICALTPFIAMCGFRPYEEIIRNFQILGI
                     DNLWPALSSFQTKPCSKNLQELFNHILTSEPSQLETTLSAMENCKNNISPKFRFTCQI
                     CKHLNEFYPQDSGVLSPLLLNTFTLQPGQAIFLEPGILHSYIQGAGIEIMANSDNVIR
                     GGLTPKHIDRDILFAITRFETYLPKLLHPHLKLNELVPYPNPAEEFHLYRLILEGNYI
                     LTNQYKPMLVFCFRGSLNTESGLSLQRGKAMFIPSEEKMLQISGSADLFIASTY"
     gene            complement(14026..15489)
                     /locus_tag="PKM80_00310"
     CDS             complement(14026..15489)
                     /locus_tag="PKM80_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNV92378.1"
                     /translation="MPPLIQNILNSLVYDPQQPLLFHSTFFLFFFIVVLLFYPLVVNK
                     VKVRTWYLMLASLYFYYKTSGSFIILLIITSLINFGLGALIYRAKSKAGKRFYLILSI
                     VWNLGSLGYFKYTNFVISTVNQIFDGQLPLYDIVLPVGISFFTFQTWSYTLDIYFGKL
                     EPLKSFVDFTFFVSFFPQLVAGPIVRASYFIPQIHKKLSLDKDTIAKALVLIFSGLLK
                     KGVIADYLSINFVDRIFENPALFSGVENLLGVYAYALQIYCDFSGYSDIAIGLAALLG
                     FSLPVNFITPYRATSVTDFWRRWHISLSTWLRDYLYIPLGGNRKGKPRQYLNLMITML
                     LGGLWHGASWNFVFWGGIHGIALVLDKMILKLKIMQTKVMKFVGVIVTFHFVCFGWIF
                     FRSPDFSVAWLILKRIFTDFHGNIFFQWISQYHVCAALILLGYIFHWMPEAYNNNLEK
                     FFRKCPLVLQSLLLAFLIWVLFQFRSAAIQPFIYFQF"
     gene            complement(15499..16767)
                     /locus_tag="PKM80_00315"
     CDS             complement(15499..16767)
                     /locus_tag="PKM80_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNV92379.1"
                     /translation="MLALAFLMLQNNSRAEDNITVDNTTELFTEEEYPSDMDTLEEFI
                     QSYYLLLESNEYLKQFKFINPEKNYISGDKKALQSFYEKLLELRSGLRDRVTIYQIGD
                     SHIQSGYFSGTARTSLQKYFGNSGRGLIFPLRLAGTNQPDDYRITASGAFSRSNSERG
                     LSGYALNATNGMCLEITTNNFFKGDNSFNTVSMITAMPGLKPKIDLAEKNIKEYSIKA
                     GNYFYSRVSWDKAVTKIKLTLAGEPSTLFGISLENNQPGLLYHSCGINGAGFYNLTAQ
                     STLFEQIGLLNPDLIIISLGTNDAQGTYRNEVFSKNLISFMDKLKQYNPETPVLFTLP
                     PDSYKRGKHNSDLDKVEKVIIDYAKENHYAWWNLSIVMGGNNSVQKWRNQQMAANDML
                     HYTPKGYMLQGYLFYQAFIKSYKSFSEKAG"
BASE COUNT         5626 a         2764 c         3187 g         5206 t
ORIGIN      
        1 tcaccccgtt gaactttagc atttctatcc agaattaata tccctctcca gggaccattt
       61 agtttttcac taataaaaaa accgccttct cgataatcag tagcagtaac aattccttct
      121 aaagtaacat ctttacccat atatggagat ggaaaagttc catcgcttcc tcttgaagaa
      181 gtgaactgaa cttcatatat agaaatggct tctacctgac aaacagcgca agccagaata
      241 ctgattagga ttaatattct cttcatctat acctctataa ttacataata aagcattcca
      301 ctatcctgtc aagtaaaatc gtaggcttaa aaatggggaa aaggtaaaaa agttaaatca
      361 ggttttcata tcgttatatc tttcatttca caagggactt acgcttacct cgtccataat
      421 tgttcaagga ttcttatgtg ataacaggtt caatttggca aattcaacag agaaaaaaag
      481 taactcaaat tctgctaacg attcttatta actatcatca tcgaaataat tagtataaga
      541 ctagtaattt aaaattacga gtaaattcac cgaattttac tgagccaata taaataccct
      601 tagataaagg tctattatta ttatcaacaa gcgagagagg aacttctaat ttacctgtat
      661 ctataattgg cagtatttct gttcttagct tttgtccctt gaggttgtat agacaaaatc
      721 tccccgaaca ttttatagtt gcttccagat ataaggttat tttatctttt gctggattag
      781 ggtaaaaaga catatgaact tgaggtatat tttctgaact atcaaaaata gcaaggggag
      841 ttctgcgtaa aataattcca tcatacgcgg gtatagatac agaagtgcat atttgaccat
      901 tgtttacaat atgatcctga ctaccattaa gatgatacca aggatgggtt aattctgcag
      961 taagagttat gttatacgga ttacatatta ccaatccatt ttcaaattct ctcataagta
     1021 attcttcctc cattaagcgt aaacctatgt catcaaaata tacaattccg ggttcattac
     1081 caagattaaa ggaaaagcga agttgatcag gatttaagct tgtgttttga gaggtaacag
     1141 agaaaatata atcagtccaa tcagtattta attcaattgg aatactatga ggaataaccc
     1201 attcccagtt ggcaagatgt tttgagataa caaaattgat tgttctgggc tgagcagatt
     1261 ttgctttgaa agtaaccaga tatctattac cgtattgaaa atccatattg ggattattat
     1321 attgcataaa ttgagcgctg taatcatttc catccgcata ggtgacatta catattgcaa
     1381 atgaattatt attttcaaat ccattttgta aatatcctgt agtgggtgat acaaatgcca
     1441 attcccaacc cgctaaatta gaattcattt ctccattaat caacaaattc ggctggtctg
     1501 aaatttgggg atttacaaaa ccttctaatg gctcgcccaa atcactatca tattcatcaa
     1561 accaccatgt atcagaatga tcagaagggc caagatcaat tgcataataa gcagaaccca
     1621 tcaatgccgt tgtaagagta aagcgaaaat gagaaatatc agcaagaaac ccatttggtt
     1681 ccatagcttg aatgatattc attactggcg ggacgaatgt atcttctatg ttattataca
     1741 tattcataaa tgttgaccag tcgtaaccaa attcaaaatt aggattatta tagtcccagc
     1801 cctcaaacat aactccatta agatattgtc catattcata acctccattt ccgattagaa
     1861 tcttagtggg attttgaatt ctaaattgat ttaacattgc ttccattccc aatatccact
     1921 gactatcaag ccaatccctg ttatcaacta tcccgtcatt attacaatca atattggcaa
     1981 tctcgtaggg gacaatagac cagcaattgt ctaaaaaaaa accatcccaa agggggttat
     2041 taagaacatt gttattaata aaatctgcaa aatgttctat ccaggtaaat ccatttactt
     2101 caggacaaaa aggagtacaa tttaacatcc aggttccttg ccaaaaagaa gtatgttctc
     2161 ctgttgccgt ccttaaccac cattcatctt gaattgagtt tcttaaggaa tagcgaaggg
     2221 gctgataaat tggattggga atatttgtac taatttcttg gcaggtcata taagccagca
     2281 gttttacatt ggggtttagt tgccttatac ttagtaaagc ggtaggattg atttcactca
     2341 tttccatatc cagaattact aaatcatatt ttgaaagtgc ggatactagt tgactgttct
     2401 ggtcactcat aaaacaatca agataataac aggataattt gggataatta cccataatat
     2461 aaactgggca taatagaagt actaaaagcg aaatgttcat aaaaaatttc cacatatttc
     2521 cctctctttt ttttacctgt gttcataaaa taattataat cgattatgga gaaaaagcaa
     2581 atagagtaaa ttgatgggga ttcgattata attaatactc actgttaata aacgaagtaa
     2641 ggaagcgaaa aaattatttc gttactgatc actcataaca tataatcctc ttactggata
     2701 tatgtctcta ttatacattt tcattctgaa acagtcgtca tttttttttc atttgacagc
     2761 cagccaaatg agaaaagact tgccctaaaa tgaggtgaaa tttgaaaaca gctattatta
     2821 ttaaaggtgc tcaggaacat aatctaaaaa atatagattt aacaattcca cgcaataccc
     2881 ttgtggtctt tacaggtgta tccggttcgg gaaaatcatc tttggctttc gatacactat
     2941 atgcagaagg ccaacgtcgt tatgtagaat ctctttctgc ttatgcaagg cagtttttag
     3001 ggcagatgga aaaacctaag gttgactaca ttgaaggtct ttctcctgca atttctatag
     3061 aacaaaaaac cacaagtaaa aatccacgct ctacagttgg aactgttact gaaatttatg
     3121 actatttaag agtcctattt gctcgaattg gaaaacagta ttgttacaat tgtggtaggc
     3181 ctgttgggtc gcagactatt gatcagatga ttgaacatct gatgcaaaat aaaacgggaa
     3241 caaaattgca aatccttgct ccccttgtac aaaatcgaaa aggtgaacat aaagatgaat
     3301 tagaaatgct tcgtcaggaa ggttatgtcc gcgtgattat caatggacag ctgcgcaatt
     3361 tggaagaaga tattattctg gataaaaaaa gcaagcacaa tatagatgta gtagtggatc
     3421 gcatagttat taaagaaggc attgaaagtc gtctttccga ttctttggaa ttagctttaa
     3481 aactggcaga tgggataatt aaaattgatt atccagaaga aaaacaagaa gttattcttt
     3541 cagcacaaaa tgcttgtccc cattgtcata ttggttttga agaactatca cctcagagtt
     3601 tttctttcaa tagtcccatt ggtgcttgta ctctttgtgg agggctgggt tataaacttg
     3661 aatttgatcc agatttggta attagtgatc ctggattgag tattatggaa ggtgctgttg
     3721 ctccctgggg acgtctagag gctaaacaac atagttggac aatgaataca gtaaagaatc
     3781 ttgcaagagc ttttgatttt tccctttcta cttcctggaa gaatctcccg gaaatagcta
     3841 aacagcttat tctatatggt tccaaaggta aaaagttcaa aaattcctgg cagaatgaaa
     3901 atggcagagg tgaattctta atgcgttttg agggtgtaat tcctcagctg gaaagaagaa
     3961 tgaacgaaac aacatctgaa gaaatgcgcc gatattatct tcaatatatc ggtgataaac
     4021 cttgccctgc ttgcaatgga aacaaattga aaccggcatc ccttgcagta aaaattgctg
     4081 gtaaaaatat cagtgacata acagcaatga gcatcagtga agcatatgac ttttttgctg
     4141 gtcttcattt gaaaggaaat gaactgctga ttgctgaaga agttttgaaa gaaataaaga
     4201 accgtttgag ctttttaaag aatgtcggtt tgcattattt aactttggat agaaggtctc
     4261 ccactctttc tggaggtgaa gctcaacgca ttcgtttagc tagtcaaatt ggcagtcaat
     4321 tagttggtgt gatgtatatt ttggatgaac ccagcatagg tttacaccaa agagatattt
     4381 ctaaacttgt ctctatgctt ttgcagctta gagatttagg taatactgta atagttgttg
     4441 aacacgatga atatacaata cgcacagcag atcaaattgt agattttgga cccaaagccg
     4501 gaatttttgg aggggaaatt gtggcttcag gagatttgga aacaattatg caccatccaa
     4561 aatccttaac tggagcatat ctttcgggaa gtattaaaat cccgactccc gaaaaaagaa
     4621 taaaacctga tgcaagaaag cttagcatta tcaatgcgag gcacaataac ctgaaaaatc
     4681 ttaatgtaga cattcctctg ggactttttg tgtgtgtaac aggtgtatct ggaagtggaa
     4741 aaagcagctt agtaaatcag actttatctc cctatctaca taatcatttt tatcatacat
     4801 cgcataaagt tggtaagatg gatgaaatac gcgggattga atatatagat aaaataatta
     4861 caatagatca acaacctata ggcagaaccc ctcgttccaa tccttctacc tatgttaaat
     4921 tattcgatcc gattcgccaa ctttttgctc agttacctgc ttctaaaata cgaggatata
     4981 aacctggcag attttccttt aatgtaaaag gtggacggtg tgaggcctgt gaaggtgcag
     5041 gtgtaaaaca gattgagatg cactttatgg ctgatatttt tgtaacctgt gaggtctgta
     5101 aaggaaaaag gtataatcag gagacacttt ctattcgcta taaggggaaa aatattgctg
     5161 aagtgctcga tatggatgtg caggaagcaa tcgagttctt tgatgccatt cctgctataa
     5221 aaagtaaact gaagacctta caacaggttg gtctcgatta ccttaaattg ggtcaaccct
     5281 ctcccactct gtctggaggc gaagcacaac gcataaaact atctcgggaa cttagtaaag
     5341 tgtctacagg taatactctt tatgtgttag atgaaccaac aacgggactt cactttgacg
     5401 atattaataa actattgaag gttctgaaaa aattggtggc aatggggaat acagttatcg
     5461 tgattgagca taatatggat gtgataaaat cttctgactg gataattgat ttaggacctg
     5521 aaggtggtga tgaaggtggg aagataatat gttgtggaac tcctgaagac cttattaatt
     5581 gtcccaattc tgcaaccggt atacatttac aatatcacat gcaaaaagaa aaggaacttt
     5641 cttcaaattc cttatctgaa ttagaaacag aaatgagacc tcgcaaagta tcaactaaaa
     5701 gaaaaacaga taaaagccag gcattatgag ttatcagcgt ttcagttatg ctccgcccac
     5761 ggcaggaaaa cattcagttg gtattgcagg tgattttagt tcctgggaaa ttatctctat
     5821 ggaagaaaag gggggagtac attatcttgc cttagaactt ccaccaggag tttatcaata
     5881 taaatttgta gtagatggtt cctggattct ggatgaaaat aatcccaaaa aggttcctga
     5941 tggttttgga ggatataatt ctctgttggt tgttgaacct gttacggaag taataacttg
     6001 ggaagatata atggctgaac tcgcgaataa aactccagaa aagttttatc aattctttcg
     6061 ttcaaaggaa aatagctatg agttgcgttt cttctggtat ccagaattgt cagatgcaat
     6121 ttatctgcta tataataatc agcaaattca gcttgcacac atcggccaaa ctcctttata
     6181 tgaggtattt tattgtttat tcgaacattg tggcattttt acttttcgcc taaaaatcca
     6241 atatcagaat atgtttctgt ttttcggagc tgagggcttt tctgaagctg aagaagatat
     6301 aactcctatt gaaatagaac taaaagctat ctccattttt tccattccag attgggttgg
     6361 ccacagtata atttatcaga tatttcccga tcgcttttat aatggaaata aggataacga
     6421 tcccgatttc agagaatggt attatgagga ttgtagaact ccaccttcct ccagtaaaat
     6481 tctttctccc gaaaaggaat attatcatat ggtagatgat tggtatgata tcaatggctt
     6541 gaaacaaagt ccttggcaaa aaaagggggt gccggatttt tattcttttt atgggggtga
     6601 tatcgcaggt gttaaacaaa agcttgatta tctacttgat ttaggtataa atgttattta
     6661 tttcaatccg ctgtggcaag cgaaatctaa tcataagtat gacagcgcag attatcatag
     6721 tatcgatccc cattttgcta ctactcagga aatgatagat tttgtaaagc tagctcacga
     6781 aaaaggaata agaatcattc tggatgtagc ttttaatcat acgggagaga cattttgggc
     6841 ttttcgtgat tgcgttgaaa aaggtccaaa atccccttac tggaattggt atgattggaa
     6901 aaaatggcct ctacccaaac ctcttcctcc ggattttaat cctaaagatt attatcaatg
     6961 ttggtggggt attaaagata tgccggattt gaattatgac cttgctctac cgcatcctga
     7021 agaaaatgct gttcgcaata taaaggaagc acatcctaat gcagctttgg ttgattatct
     7081 gatgtctact gttcgctggt ggttaattga tattgggatt gatggtttcc ggttagatgt
     7141 tccagatgaa gttcctttct ggttttggga actgtttcgc aaggaagtaa aaaaagctaa
     7201 acccgatgct tggatagttg gtgaaatctg gtatgaagca agacagtggg tttcgcctct
     7261 ctatttcgat tccgtgatga attatgcatt ctttaaaaat ccggtaatcg agttctttat
     7321 tcttagcttg ctaaataagg aagaattctg ccgaaaaata gaatccggac tcgctctata
     7381 tcctttccat tccttaaagg caatgatgaa tcttttaggg agccatgata cacccaggat
     7441 ttttgagata agtaaaggag atgttaaacg cctgaaacta gcaattgctt ttcagatgtg
     7501 ttttattgga gctcctcata tttattatgg agatgaaatt gctatggaag gaggcaaaga
     7561 tcctgataat agaagacctt ttaactggaa ttgggaaaat aatccggtag ctggtgactt
     7621 aagaaacttc tataaagaac taatttctat ccgtaaagct catcctactc tttgtgaagg
     7681 acattttgct tttatcccag ctccagataa aataattgct tttcagcggg aattgaaagg
     7741 tgaaaaaatt ctggttttct tgaataccga agcagaatca atagctattg atatcccaga
     7801 atcctcaata ttacttttag ggaagatagc agagaggaat tatatattac ctcattctgc
     7861 attgatttta tcagagttga aatagagttg cttatagata gtgaaaatat attgatgctg
     7921 aaataaaatt cgagaaaaag tgatgcctaa gctgttcagc aaaagagaac atgggatgta
     7981 tcgatagatt gttggataga aaagtaattc aaatatcttg acttcaaacc agcaatggta
     8041 aaatgataat atagaataag gagtatgaat gtgcggaata gcaggtatat ataattttag
     8101 taaagagaca gttattttaa ctgaagtaac tggaaaaaaa ggtatattaa atacagaacc
     8161 agcaggttta agtaacgaca tagataattt agtaaaaata gagggtggtt atagacaaaa
     8221 cggagctaag gcaattctat taaagaaaat gactgaagca atcaagcatc gtggtcctga
     8281 tgatgagggc tatctaataa aggataaaaa tggaatcaga tcttattcag gaatggattc
     8341 aacggaggaa ataaaatcgt tatatccttt atttaattca gaaataacta ctttagataa
     8401 cctttctaaa aaagctgtgg gaaagacagt tggagcagag aacataatct tgggaatggg
     8461 cttccgccga ttgagtatat tggaattgaa accgattggt catcaaccga tgtatgataa
     8521 ggaactgggc atagcaattt gttataatgg agaaatttat aactatcttg aactgcgtga
     8581 agagctaaag aagaagggct ataaattttg tagtcagagc gatacagaag taataataaa
     8641 agcatatcac ttttggggtg aaaattgcgt gcagcatttt aacggaatgt ggtcattttc
     8701 tctatgggat gagaaaaaga acttgctatt atgtagtcgt gatcgctatg ggataaaacc
     8761 attttattat gcggttcaga atggagtttt atattggggg agtgaaatta agcaacttat
     8821 gctcactccg atagacaaaa ctctaaacca agcaatgata tggcgtagta tgaagataaa
     8881 ttctatgctg gtttatgatg atgagaccta ttggcagaat atacactgtt taaaaccggg
     8941 acataatttg atagtaacta acagtaaggt tattgtaaaa caatattatg atttggatat
     9001 aacaagtttc gaaacttctc agctatcttt taatgaagca gttgagcaat atcgaaatat
     9061 ctttctagat tccattgctc tacaacttcg cagtgatgta gaaattggag caactctttc
     9121 gggaggaatg gactcttctg caatcgtgtg ttctgcagtg catcaaaaga aagagccgct
     9181 taaaacattt tcatcttact atgctttccc tccagaattg gatgaacgca agtggataga
     9241 gaaaattatt aaagaaactt cctgtgcttc atatttgatt agccctaaag ctaatgatgc
     9301 tatagattta tgggagaaat taacattctt aaatgatcta cctttagccg caggttttgt
     9361 atcgcaaaat gcagttttgc aagaagctca taaacaaggg attaaagtta tactctctgg
     9421 tcagggatca gatgagctat ctgctgggta cagacattct ttatatcgct attttgctga
     9481 tctgataaga ggtgtgcata ttagtagact aggaaaagaa cttccgggat atttgaaaaa
     9541 agacaatcgg gcattttcca agctgagcaa aatagtgctt tctacttttt taccagaatc
     9601 ctcactatat aatttggagt tttgctatta ccggtttgaa cctttcacgc gttcttttac
     9661 aaatgaagct gaaatttcct gtggtgagca gatattaaaa aagatagtag atataaaagc
     9721 ttcgcggctt tccaatttct tatataatat ggtgcatact acttctctgc aaactctgtt
     9781 gcattttgaa gacaggatgg caatggcgaa ttctattgaa agcagagttc cttttctgga
     9841 ctatcgttta gttgattttg ttttttcgct tccttctcaa tataaagtta aaccccctta
     9901 tacaaaagta attcatcggg ctgcaatgaa agaactgatt ccggaagaga tttatcagcg
     9961 cacagataaa gggatttttt cttcaccatt ttatcagagt tggatgaaac aagaattaaa
    10021 accctatata tcagatattt ttgccagtag tacctttagg cagagaggat tatggaattt
    10081 gcccaagatt aatcattttt ggcataaata tttaggggga gataacagtc aggtggaaat
    10141 gttatataat gttgttgccc tagaaatatg gtttcaacag tatgaaaatc aatctacagg
    10201 aggattaaaa tgaaaaacat agttaaaata atagctttat gctttgttct aatttctctt
    10261 agttcctgta aacttttcaa aagtccaaca gttactaaaa ttcaagatgt gaaagttgtt
    10321 tcaataaccc ctgataaaag cacaataaaa atatccctta tggtgaataa tcccaattgc
    10381 tataaactga aactggataa actggaagta gagttactga ataaagaccg tgacaaagtt
    10441 ggttatgcta atttagagaa agttgtggaa atacccaaaa aggcagctat gtgtataaat
    10501 tttaatgttg agctggaaac gcgtccggta gtgagaatga ttagttcgct taaccagaaa
    10561 gttcaatttt tcattgtagg taaaggacac ggcaaagtta agggtcttag caaaaaattc
    10621 tattttgatg agccttacga gctggatttg aaagaagaat taaaaaatat tattcccaaa
    10681 ttcagtgtag gtacccagaa tctgtttaaa atcacgaaaa ccagcgtgga gaagatgagc
    10741 atcggtcaaa ccgaattaca tatccatttt attattttga atccttatgg ccttacttat
    10801 aatttaaaag gttttcctgc tgagatttat attgatgata aattagctgg aacgggaaac
    10861 ctgaaaaacc agctgaaatt caatgaaaat atcttctcac aagatgggat aatggtattt
    10921 caactaaata acttgaaatc catttttggt gctgtgaaag gtgcgattaa aggtgaaatc
    10981 aaatactctg tcaaagggaa tgtaattatt gatgcttttg gtatgcaaat aaagcagccc
    11041 tactcatacc aggattcctt acctgtaaat attgtggaat tgatactgaa ttaaaactga
    11101 tgcttgtttc gctttgacta tcaaaatggt agcgattaaa agaaagttct agtgtagtgt
    11161 ataaaatcct ggaagcacga ttttgcttga cattttccta aaagcgctta ttataaattg
    11221 taatgatact taagccaagg agataaagat gaaaaaatat ttgtttttgg tattgattat
    11281 attacctgtt ttcttactag ggcaagttag tagtgaggaa acaaatattc ctggagattc
    11341 tatgccagaa gaagaatcgg aatatccgga aggaactcaa tggggtatgg gtggcgcagt
    11401 tggtacagta acaattgata atcgaaccta ttcccagatt cgtttacaac cagaacttca
    11461 gattggtaaa ttcggaattg gtctggatat tgatctgatt attgatgctg aaggtaatgt
    11521 tcgtaaagag gattgggatg aattgcagga ttatgtaaat aaatttttat atattagatt
    11581 tgcgcaacgg aatgatccct tctattttaa aattggtagc attccaagtt atacgctggg
    11641 taatggttta attttcaaca attattccaa tatgatactc tatccaagtg tgaaaaatgt
    11701 agggggttat ataggtgtta ataccaatat atcaggtctt ggttttgaag catactccca
    11761 taacattcgc aagaatgaaa ttttagcagg aagagtgcat attcaacctt tggcagcaac
    11821 caacattcct ttactagaaa aattgcggtt aggagttaat gctggaatag accgtgatca
    11881 aaatgctaaa tatgaagata aagatggtga tcatattcct gatatttacg ataaatttcc
    11941 caaggataaa atgcgatatt tagatactga taatgatggc attgcggatg aagatgatat
    12001 agacatagac ggagatggta ttttagacca tcctgattat aacccttata tagcacaaaa
    12061 ctttcctgga attgaagata tcgctcaggg ttataatttg gatattgtta tattccccga
    12121 tacagcagcc gcgtataata aaaaggatga aattctaatc tacagtgcag attatcagtt
    12181 acctttaatt gaaaccgacc attttaacct ggttaattat ggtgaactag ccaaaatcga
    12241 cggttacggg actggaatga tttttcccgg gtttagcagc aaattcttca tttttgacgc
    12301 aaaagtggaa ttccgtaatt ttggtgatca atttattcca ggatattttg ataaacaata
    12361 tgatcagcag cgagctgaag tgaaacatat agttttggag gaaagcggaa gccagtattg
    12421 gttcttaact accaaagaat ccaatttgga gaatgttgaa gcttctcttg gctggtttgg
    12481 ctatttacgc gctaatctct ttgatttcat ctatcccaaa atcgcctatc aagatatgta
    12541 tgggaagaat aactttctgg gaaaatccct atggggtagt attactataa atccatatat
    12601 gattcccaaa ttgaaagaag caactatata ctattcacaa accaatacta actatataaa
    12661 tttccgatat ctacgtaatg aaaacgctta catttcagga cgcttagttt attcattatc
    12721 cgataatgca aatctaattg gaaaatacag cgaattctat acagatatca attgtgacgg
    12781 taaaattaaa ggtaatgatg aagtaattga atcctttact tttggagtgg agtttcaatt
    12841 ttagacttca ataagttgaa gctataaaaa gatcagcaga accggaaatt tgaagcattt
    12901 tttcttcaga ggggatgaac attgcttttc ctctttgtaa tgataatccg ctttcagtgt
    12961 tcaatgaacc ccggaagcaa aaaacaagca taggtttata ttggtttgtt aaaatgtaat
    13021 taccttccaa gataagacgg tagagatgaa attcttctgc aggattagga tagggaacaa
    13081 gttcattcaa tttaagatgg ggatgtaaaa gtttgggaag ataggtttca aagcgagtta
    13141 tagcaaaaag gatatctcta tctatatgct ttggggttag ccctccccga ataacattat
    13201 ccgaatttgc cataatttca atgccagcac cctggatgta tgaatgcaaa atcccaggtt
    13261 ccaaaaatat agcctgacca ggttgcaggg tgaaggtatt tagtaaaaga gggctaagta
    13321 caccgctatc ttgtggatag aattcattta aatgtttaca aatctggcag gtgaacctga
    13381 attttgggct aatattattc ttacaatttt ccatagcaga gagagttgtt tccaattgag
    13441 agggctcgct tgttaatata tggttgaata actcctgtag attcttagag caaggtttcg
    13501 tctggaaaga agataatgcc ggccagagat tatctatccc caggatttgg aaattgcgaa
    13561 tgatttcttc ataaggtcta aatccacaca tagcaataaa aggtgtaagg gcacagatta
    13621 gttctggttt tggattaggg tctttaaaaa tacgattagg atcagttatt ggtattcttg
    13681 cctcttcttc tttttggaag ccatattgtg cagtttgcct atccgggtgt acttgtaatg
    13741 aaagtggttg ggcagccgca aggattttaa ataaaaaagg tagattatta ccctgatttt
    13801 tacccaacat actttccgga tatgtttcca ataacttatc catagggatt tcaccttctt
    13861 cagttataat aagagatggt gattgaggat gtgctccgaa ccatgcttca gctaagggtt
    13921 ttcctgtcat atccagagca ccaattagct cttgaatata ttttatatca ccccaagaat
    13981 agtcttttaa cgagggttta attagataag gcataaatca tctcctcaaa attgaaaata
    14041 aatgaaaggt tggattgctg ccgaacggaa ttgaaagaga acccagataa gaaatgctaa
    14101 caacaatgat tgtagaacta atgggcattt tcggaagaat ttttccagat tattgttgta
    14161 agcttctggc atccagtgaa atatataccc gagcaaaatt aaggcagcac atacatggta
    14221 ttgactgatc cattgaaaga atatatttcc gtgaaaatca gtaaaaatgc gtttcaatat
    14281 taaccaagcg acggaaaaat cgggagaacg gaaaaatatc caaccaaaac aaacaaaatg
    14341 gaaagttact atcactccca caaatttcat cactttggtt tgcataattt ttaactttag
    14401 aatcatttta tccagcacta aagcgatacc atgaattcca ccccaaaaaa caaaattcca
    14461 agatgctcca tgccacaaac cacctaaaag cattgttatc attagattta aatactgtct
    14521 gggtttgcct tttctgtttc cccccaaagg aatataaaga taatctctta gccatgtgga
    14581 aagagatata tgccaacggc gccaaaaatc ggttaccgaa gttgcccgat agggagtaat
    14641 aaagtttacc ggcaaagaaa aaccaagaag agctgccaaa ccaatggcaa tatctgaata
    14701 accgctaaaa tcacaatata tttgtaaggc ataggcataa actcctaaaa gattttccac
    14761 accggaaaat agtgctggat tctcaaagat gcggtcaaca aagttgatag ataaataatc
    14821 tgcgataaca ccttttttca gtaatccgga aaagattaat acaagagctt tggcaattgt
    14881 atccttatct aaacttaact tcttatgaat ttggggaata aaataacttg cccggacaat
    14941 tggtcctgca accagctgtg gaaagaaaga tacaaaaaaa gtgaaatcca caaaactctt
    15001 gagcggttca agcttaccaa aataaatatc cagagtgtaa ctccaggttt ggaaagtaaa
    15061 aaatgatata cctacaggta aaacgatatc atataaaggt agctgaccat caaaaatctg
    15121 gttaacagta gatatgacga aattagtata tttgaaataa cccagcgaac ctaaattcca
    15181 tacaatgcta agaataagat aaaatcgttt tcctgctttg cttttagctc tataaataag
    15241 tgcacccaag ccaaaattga ttagggaggt tatgataagt aaaataatga aactaccgct
    15301 ggttttgtaa taaaaataca aggaagccaa catcaaatac caggtacgga cttttacctt
    15361 attcacaacc aatggataga aaagaaggac tacaataaag aaaaatagaa aaaaggtgct
    15421 atggaaaagc aacggctgtt gtgggtcgta aactaaacta ttaaggatgt tttgaattaa
    15481 gggaggcata atttattctt aacctgcttt ttcgctaaaa ctcttataac tttttatgaa
    15541 tgcctgatag aagagataac cttgcagcat ataacccttg ggagtgtagt gaagcatatc
    15601 attagctgcc atttgttgat ttctccattt ttgaacacta ttgttgccac ccatcactat
    15661 gcttaaattc caccatgcat agtgattttc tttggcataa tcgataatca ctttttccac
    15721 tttatctaaa tcgctattat gcttgccccg tttatacgaa tcaggtggta aagtaaaaag
    15781 aaccggtgtt tcaggattat attgtttcaa tttatccata aaagaaataa gatttttgct
    15841 gaaaacttcg tttcgatagg tgccctgagc atcgtttgtc cccaaagaaa taataattaa
    15901 atccggattc aatagcccta tttgttcaaa taaggttgat tgagctgtta aattataaaa
    15961 acctgctcca ttaattccac aagagtgata aagtaatccc ggttgattat tttccagaga
    16021 gattccaaat aaagtggaag gttctcccgc aagtgttagt ttaattttgg taactgcttt
    16081 atcccaggaa actctgctgt aaaaataatt tccagcttta atagaatact ctttgatgtt
    16141 cttttccgct aaatcaattt ttggtttcaa accgggcatc gcagtaatca tactaactgt
    16201 gttaaagcta ttatcgcctt tgaaaaaatt gtttgtagta atttccagac acattccgtt
    16261 ggtagcattc aaagcgtaac cacttaaacc acgttcggaa tttgaacggc tgaaagcccc
    16321 gctggccgtt attctataat catccggttg attagtccct gctaagcgta aagggaaaat
    16381 aagccctcta cctgaattgc cgaaatattt ttgtaatgaa gttcttgctg ttccagaaaa
    16441 atacccagat tggatatggg aatcacctat ttgataaata gttactctat ctcttaatcc
    16501 actacgcaac tccagcaatt tttcataaaa actttgcaac gcttttttat cacccgatat
    16561 ataatttttc tctggattaa tgaacttgaa ctgtttcaag tattcgttgc tttccagaag
    16621 cagatagtag ctttgaataa attcttccaa agtatccata tcagaaggat attcctcttc
    16681 agtaaaaagt tctgttgtgt tatcaactgt tatattatct tctgcgcggc tattattttg
    16741 tagcatcaaa aaagctaagg ccagaattaa aacagagagg aaa
//
LOCUS       DAORHB010000005        16349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_2642_length_16349_cov_4.795508, whole genome shotgun sequence.
ACCESSION   DAORHB010000005 DAORHB010000000
VERSION     DAORHB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 16349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16349
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2642_length_16349_cov_4.795508"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PKM80_00320"
     CDS             complement(<1..1401)
                     /locus_tag="PKM80_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92380.1"
                     /translation="MKKRVYLILAILILMLGSLSAKHSDKYLLANYTLLRCYQNVPGW
                     YYTLLDSIQAAKFNASVITVMPGDFPKRTDLLFNAMDERGLDVLHYDMAFNPDSKNPE
                     YGIEAFSIANYWRFEAEYDSTFKENLLDDKYFYNNSLTTGKQVYNELASGKYLLQLNN
                     GQPGYAFNKLEFRWQDKGYTNYLIGNEFRFIQREWAGNFKANPSVNDTLFVTIAFKCS
                     NIPTDPNAELMQFSFNGFDNTKNEHKVPHLNTLSSESGFTSKLTVNQYNKLPLISKDE
                     QGNMLWPHKEITLKVSLQDLHDAGLLEGGILWKYLLSNLNPQIYWNGKGILELDYVEF
                     EDTMNKRQKTDTELIQAVRKRVYDFAERYDNIKYFYLTDEPTQGQFSSYKRIHKDIFK
                     DIKHLTGGSSLFTCSILHRKYVIKPNNMMYDHIGLYSREVDPEIISFDICPLWGWIQW
                     NNPEERRGIQRRLHYDM"
     gene            1984..3021
                     /locus_tag="PKM80_00325"
     CDS             1984..3021
                     /locus_tag="PKM80_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNV92381.1"
                     /translation="MSIFDFFGMMANDIAIDLGTANTLVYKKNGGIVINEPSVVAVST
                     DNKKIIAIGNPAKLMLGKNPDEVRVIKPLKDGVIADFQVTELMLRNLILRAQKKRLLV
                     RPRVIVCVPSGITEVEKRAVRDSALHAGSREVHLISEPIAAAIGADLPIDMACGNMVM
                     DIGGGTSEIAVISLSHIVVHNSIRVGGDQIDMDIINYLRKKSNLLVGPQTAEKIKMTI
                     GSAYPLKQELSMDVRGRDIISGYPVTIKISSEEIREAISETVATIIDAIKRLFERTVA
                     ELSADIAERGIFLTGGGALLKGLDEKIRKTVDLPVHVVPDPTECVVKGAGKVLDDVDR
                     YRQVLIKRIED"
     gene            3080..3847
                     /gene="mreC"
                     /locus_tag="PKM80_00330"
     CDS             3080..3847
                     /gene="mreC"
                     /locus_tag="PKM80_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNV92382.1"
                     /translation="MFKKNLVPLILIVLSLLMVIGSKESRIKKAGWMGNTVFFPFVKS
                     LKAIETTQELKQEVFSLRKQLAETTLEKLELQNQLKQFTGAYSISYDLGEVELIQAEV
                     IGLAGQYQELNLVVDKGFKFGIKINDPVISASGIVGKIIQVAADHSIVLPFSNPRFQL
                     PVMDARTSVQGILQSDISGTVSMNLIKLGSDIAVGDTIVTSNLSRLYPKSYPVGTIKR
                     IRESQDNLFISADINPFTLVENLEHIFILKRQEQIEL"
     gene            3837..4529
                     /locus_tag="PKM80_00335"
     CDS             3837..4529
                     /locus_tag="PKM80_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92383.1"
                     /translation="MSYRKKTEPVVTHWGVRFERIPVKTKILTPQDKISDIIKEYALP
                     LMKPGDILTISESPLAITQGRAIPVSEIRVSPLAKLLSRYVAKVPYGIGLGAPTSMQC
                     AIEETGIMRILFAAFIGAIGKLIGRKGDFYRVAGMQAALIDAATTSPVPPYTDTVIKG
                     PKNPQQICEDLHSEFGYEFAVMDINDIGGCWMIGGSKGVSKHFIESVMKDNPQGQGDE
                     LTPFCIVRRVDN"
     gene            4529..5023
                     /gene="mreD"
                     /locus_tag="PKM80_00340"
     CDS             4529..5023
                     /gene="mreD"
                     /locus_tag="PKM80_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNV92384.1"
                     /translation="MVWKGIWSFILGLFFLYLQILVMPALALFKAIPFILLPWMIYIV
                     WTRPRNFALIVVFIIGMMFDTVNPMTFGFYSLLFCLLVLVIQEFRKPFEAESGVAKLL
                     AIGIANFIFSVLQLVMYGVTFGFDGELLAKSLVGFGYNVIVSIVVFWSMQLISKVRLT
                     INND"
     gene            5016..6929
                     /gene="mrdA"
                     /locus_tag="PKM80_00345"
     CDS             5016..6929
                     /gene="mrdA"
                     /locus_tag="PKM80_00345"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNV92385.1"
                     /translation="MTKNQATITYSITLAVLFILLAASLFRLQVVEGAKYQRIAESNF
                     VRIRRIIATRGEIYDHKYRPIVQNVPSHNMYLTSGKINDINALSRFLEQNFGITESEL
                     KDLVLKQRFKTYEEILLADNVPYETVLALSEELNYYPELSFHIGSTRNYLYPNHFSGY
                     VGRINEKEYDLYKKEDYSLNSYIGKTGLERYYEVLLRGKDGREIVQVDAQGHSLQLFE
                     EGGNIEPLNGLSLVLTIDNDLQEFVSGIFPSGMKGAIVVSDVRSGGILAYVSKPDFDP
                     NLFMQKITPEIWEDLNRDEKPMVDRVIYGTYPPGSVFKPITAGIGLDRGLITPETHLA
                     FCSGGFKVGNRFFRCWFSSGHGSTTVVDALRVSCDTFFYDLSLKIKLDDFYNYVRDSH
                     LFGKTGIDLPNERQGFFPNTEWYKKTYGKNVGIIGHKVNLAIGQGEVLTTPLQINAFY
                     GALANNGLWIQPHLLKQTVGRGRLTREQVVPIAKRRLPMKPSTLKTIQQGLWAVVNAP
                     GGTGANAKINGATVYGKTGSAENVMGRKTHAWFCGYLVTSKPEIAVTVFLENAGGGGA
                     MAAPITAKIFNYYLGNLDIIKQVTPLPPQFRTAEGIENEEFEPGLEGSEVSIPGSEIE
                     PLPASLQEESDVH"
     gene            6919..8139
                     /gene="rodA"
                     /locus_tag="PKM80_00350"
     CDS             6919..8139
                     /gene="rodA"
                     /locus_tag="PKM80_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNV92386.1"
                     /translation="MFINRKKFDFVLLTLLLLLIIFGCIVIFSASTTVIAGEAHTQNN
                     WWKQVIFAVLSLLAIILLLWIPMPIFDLVVLPLYLLNILALIIVLFTPIINGAHRWFS
                     VGGINLQPSESAKLLTILMVARVISKENLNEYRQILYGFLLTLIPVILIMIEPDFGTT
                     LVFWASLIAMLIAADVPLFYILLCISPIVSIISSIWWPAIPIWIIIFVLLLLKARLSW
                     VTITVASIINVFIALITPVFWMGLKDYQQNRILTFMDPTRDPLGAGYQIIQAKIAIGS
                     GSLFGKGWLKGTQKNMNFLPEHHTDFIFSVVGEEFGFLGSILLLILFAAFFSRIINNV
                     GELKIRERKVASAGILAYLMFQTFINIGMNIGLVPATGIPLPLISYGGSNLLINSIAV
                     GVVLKYMNERGFMK"
     gene            8136..8744
                     /locus_tag="PKM80_00355"
     CDS             8136..8744
                     /locus_tag="PKM80_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92387.1"
                     /translation="MKNRVLGLVLLALLSFFINACSVVTTLSFTERDIKDSPNLVENY
                     SFSPYSTIGEEALKGWMVILNPHDNSESPITIDPDTACEGKTSLRIDASDKSVLILSE
                     PFNVRRYGGYYLRCYFKTNSPNPPRPQLRFIVFKENGKIVNRFKTKAKINNDWQRTTI
                     SAGFIKPGARFGRLGIYIPPFKEGSIWIDDTSCFEVHGFKID"
     gene            8744..9181
                     /gene="dtd"
                     /locus_tag="PKM80_00360"
     CDS             8744..9181
                     /gene="dtd"
                     /locus_tag="PKM80_00360"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNV92388.1"
                     /translation="MRITLQRVSKAVCYVDDKITGQIETGLLIFVGFSSFDTEAVLPL
                     AVRKCLELRIFEDENGKMNKSVSDINGSVLVISQFTLYANCQRGRRPDFTQAAPPGKA
                     KELYEEFVHLFKQTEINVQTGIFAAKMQIELVNEGPVTINLEF"
     gene            complement(9533..9820)
                     /locus_tag="PKM80_00365"
     CDS             complement(9533..9820)
                     /locus_tag="PKM80_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNV92389.1"
                     /translation="MTKADLVKIISENTGIIRKDVAVVVDAFLQAIKDSLIKGNHIEI
                     RGFGTYKLKTRKPRVGRNPKTDEKVPVPERTVPTFKFSREFKNSVLDVKTK"
     gene            10354..10983
                     /locus_tag="PKM80_00370"
     CDS             10354..10983
                     /locus_tag="PKM80_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92390.1"
                     /translation="METAILSPLSNMCTFHCKGKLVFLSYGGREHKLFYGKGMQYLIT
                     LIANPDLYISCANIETIKPMPQRVYSQFSSSNEVQIHQLEVQSSFPSIPLTDLQTVKE
                     VKQELIRVIDTLAELEANCDYAQADDIRDQYEKLVQYLEQVYKPEGKIRYFPSEGKKT
                     KKRVLRAINRSLEDIEKLEPELAVELKNSLELGTILHYHPKIDIEVLGY"
     gene            11369..16159
                     /locus_tag="PKM80_00375"
     CDS             11369..16159
                     /locus_tag="PKM80_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNV92391.1"
                     /translation="MKKNILILICLILISQFFAMAKQNEDRERPLYANNLVKIKLSSE
                     AVFRAQLPLSAYAECEKTNLNELDQLFEVNGVQSIIRAHISAKDRNWETKTGFDRWFL
                     LRLDGTKTVEQVIANLKSNRYIEDIIPEYYAYLHNVPDDPLYPNNWGHNNTAQLPVYQ
                     GDSHSGPGVGVIGFDSDAEIAWDSNQGYGDPDIIIGILDTGVDLNHPDLLLVSGYDFG
                     DNDDNPMDDSAEAGHGTCTSGIAAAKANNILGVAGIAGGCSVMPLKVASSNGDILYSA
                     ITNALIYAADNNVNVVSMSFGSNSHVGDNPTVDSALEYAYNNGVILFASTGNANSATI
                     GYPAIHPLVISVGAASPSGERKNPNSSDGENWWGSNYGTNQQDAAGAVDIMAPTILPA
                     TDITSFGGYSLDDYFMWFNGTSCSCPYAAGVAALVLSVNPTLTPAELRTIITSSATDM
                     TAGTSEGWDRWTGYGMVNVAQALCEANPSIPLCHIVYPLNNTGFEINSVITIQVEASD
                     PVERSILEVRFYLDDNPDYVFSDSEMPYEWVWNTAGLSIGQHTIKAEAIDNEGNRRYH
                     SISISLLNPADEGFETADFSKLIWLIKSSSPWTVQSDSYFTGEYSACSGLINAEGASE
                     LKLKVNITEAGEISFYRKVSSLANYGYLKFYIDDELIGEWSGEKDWACVSFPVNPGLR
                     TFSWAYQKNSSNVSGSDCCWIDHIIMPPYENYYWPPQNLTAAGGYGFVLLYWKVPEAG
                     EPTGYNILKNNVLLTTIAETRYTDTDVVAGISYEYTVVAVYAEGQSESTSAQTAIPTS
                     EVVTETIIGNGIIPTSNYEGCPINNYRKSLHGQMIYTARELNLHGIFGPTIITRLGFY
                     INTASAYSLPNFRIRMAHTTAEDVSQWLGEDDLITCYLATNYRPVAGDYDMLTFSTPF
                     LWNGIDNILVDTAFSLTVAVKSSGTVLYTDTPKGYHYTRSNVEDQTDVFTGGAVTMYK
                     PNMKLSVLTQPDIAVNTTNLSFGNVLAGYTAGKTFNITNTGTGYLNGEITVPAGYKIV
                     SLDEEYSSLSYSIRADSTAVFTVLFAPLAPGVYNGIISITHNGEGEERIINLSAVSLT
                     ALNTPFIEGFENYCENWTFVNEGQTNKWFYGFIVPQSGNGGLFVSGDNGLTNSYVPNR
                     GSTSYIYRDIYLPQGTENCKLRFSWKGMGEGTNPYFDFLRVFCVEPNFVPQAGTMISS
                     GQLGTTLNLSDHWKEADFNIPEPVSGVYKRIVFAWRNDASGETQPPAAIDNVRVILNS
                     NTDYAYVYDGSATVNLPAVSDTLGHTYHGNLIITGITEPDEYLVVTAGFASLNEPFSD
                     AGMDFTVTGANFSGAEITFNHNLGYVPYYLGYKIEGRGEWMTFSAAPNWTDKIAYFTV
                     PVYYGNATNISFCFPHANNVSPYYPELNVVLDMQNVVSIIWKCNYTEGISAYQIWRGS
                     SPSFNSAVAVSDLIPHLDTQEENYVYTDTTTDENTRYFYWLRIINIDESSSYWGPKLI
                     VTGTDPLQPPPETTLLGAYPNPFRYGTNISYTIKNAGNVEFKIYNLKGQLVNNYLQYH
                     NNYGTYIYEYTGTDKKGKELASGLYICYMKFGNKTFVQKIIKAN"
BASE COUNT         5159 a         2741 c         3213 g         5236 t
ORIGIN      
        1 catatcataa tgaaggcgtc tttggattcc tcttctttct tcagggttat tccattggat
       61 ccaaccccaa agaggacaga tatcgaaaga gatgatttcg ggatcaacct cgcgagagta
      121 taatccaata tggtcataca tcatattatt aggttttatt acatatttcc tatgcaaaat
      181 ggaacaggtg aatagagatg aaccaccggt taaatgtttt atatctttaa aaatatcttt
      241 atggatacgc ttataagaac tgaattgacc ttgggttggc tcatcggtaa gatagaaata
      301 tttaatgtta tcatatcttt ctgcaaagtc ataaactctt ttgcgaacag cttgaatcag
      361 ttcggtatct gtcttttggc gtttattcat tgtgtcttca aattctacat aatccaattc
      421 caaaattcct tttccgttcc aatagatttg gggattgaga ttgctaagta gatatttcca
      481 caaaatgcct ccttccaaaa ggccggcatc gtgtaaatcc tgtaaagaaa cttttaaagt
      541 gatttcttta tgaggccaga gcatatttcc ctgttcatct ttagagataa gaggtagctt
      601 attatattga tttactgtta gtttactggt aaagccagat tcggaagata gtgtgttcag
      661 atgtggaact ttatgttcat ttttagtatt atcaaaaccg ttaaaagaaa attgcatcaa
      721 ttctgcattt ggatccgtgg ggatatttga acatttaaaa gcaatcgtta caaataaagt
      781 atcgtttact gagggattcg ctttaaaatt acccgcccat tctctttgaa taaagcggaa
      841 ttcattaccg attaaataat ttgtatagcc cttatcctgc cagcgaaatt ctaatttatt
      901 aaaagcataa ccaggttgac cattatttag ttgcaaaaga tatttaccgg aagcaagttc
      961 attgtaaact tgcttgccag tcgttaagga attattataa aaatacttgt cgtccaatag
     1021 attctctttg aaagtagaat catattcagc ttcaaaacgc caataattag caatagaaaa
     1081 tgcctcaata ccatattcag ggtttttact atcagggtta aatgccatat cataatgcag
     1141 aacatccaac ccacgctcat ccatcgcgtt aaaaagaagg tcggttctct taggaaaatc
     1201 accaggcatt acggttatta cagaagcatt gaatttagct gcctgaattg aatccaataa
     1261 agtataatac cagccaggaa cattttgata acaccttaac aaagtgtaat ttgctaaaag
     1321 atatttatct gaatgcttag cagatagcga accaagcatt aagatcagaa ttgccaaaat
     1381 aagatatact ctttttttca tctgaacctt ccttatagaa ctcataaagt tctatttttt
     1441 cacatattat caatttatac cttaacaatt tctatttggt agctgtcaag caaatattca
     1501 agggatactt atcctaatct gatttttagc aaaaaagcgc agcttaacta aatcgattac
     1561 tggcacttcc tgcttaggca attctaaaat gatcttctat catttactaa acgggattaa
     1621 ggaagtggta gtgggatttt tttaagtaac ccaaaagcta aaagcaaaca aaaaaccccc
     1681 gcacaaagaa ctcctgcact aagaactcct gcactaagaa ctaaaaaccc ccgcacaaag
     1741 aactcctgca ctaaaaaccc ctgcactaag aactcctgca ctaagaacta aaaaccccca
     1801 cactaatttc ccaatccatt tttaacaaaa ctggaatctg ggaaatctgt aaaaaagatg
     1861 gaaaaaatgg taactgtcgt ttttacttga cacagcagga tatataaaaa atagggtata
     1921 ggtagaagaa atgttaattt ataaattcaa gctgctaagc aagcaggaaa tagagaggtt
     1981 ttgatgtcca tatttgactt ttttggaatg atggccaatg atattgcaat tgatttggga
     2041 accgcaaaca ccttagttta taagaaaaat ggcggaattg tgataaatga gccctcagtt
     2101 gttgctgtat caacagacaa taaaaagatt attgctatag gcaacccagc aaaattaatg
     2161 ctgggtaaaa atcctgatga agtaagagtg ataaaaccct tgaaagacgg cgttattgct
     2221 gattttcagg ttactgaact aatgctacgc aatttaattt tacgagccca gaagaaacgt
     2281 cttttagttc gtcccagagt aattgtttgt gttccatctg gaataactga agtagaaaaa
     2341 agagctgtgc gtgatagtgc tttgcatgca ggttctcgtg aagttcattt aatttctgaa
     2401 cccattgctg ctgccatagg tgctgattta ccaattgata tggcttgtgg aaatatggtt
     2461 atggatattg gaggtggaac cagcgaaatt gctgtaattt ctttatctca cattgtagtt
     2521 cataattcca ttcgcgtagg tggtgatcaa attgatatgg atataataaa ctatttgcgg
     2581 aagaaaagca acctgttagt tggtccacaa actgctgaaa aaataaaaat gacgattggc
     2641 agtgcttatc ctttaaaaca agaattatct atggatgtgc gtggacgaga tatcatatct
     2701 ggttatcctg taacaataaa aataagctct gaagaaatta gagaagcaat ttcggaaaca
     2761 gttgcgacca tcattgatgc cataaagcga ctttttgaaa gaacagtggc agaactttct
     2821 gctgatattg ctgaacgggg tatattttta acaggtggag gcgctctcct taaaggcttg
     2881 gatgaaaaaa ttcgtaaaac agtagatcta cctgttcatg ttgttcctga tcctacagaa
     2941 tgcgtagtta aaggtgctgg gaaagttttg gatgatgtag accgttaccg tcaggttcta
     3001 ataaaaagaa ttgaggactg atctaaaatc tgaacctatt acccaagatg ataactccct
     3061 attttaaaag ggacgaaata tgtttaagaa gaaccttgtt ccccttattt taattgtcct
     3121 ttctctacta atggtgatag gcagtaagga aagtcgtatc aaaaaagcag gctggatggg
     3181 aaatacggtt ttcttccctt tcgttaaatc tctaaaagca attgagacca cgcaagaact
     3241 gaagcaggaa gttttttctt tgcgaaaaca attggctgaa acaactttgg aaaaactgga
     3301 actacaaaac cagctgaaac agtttactgg agcttattct atttcctatg atcttggtga
     3361 agtagaatta attcaagcag aagttattgg tttagccggt caatatcagg aacttaattt
     3421 agtggtggat aaaggattca aattcggaat caagattaat gatccggtta tttcagctag
     3481 tgggattgta gggaaaataa ttcaagtggc agccgatcat tctattgtgc ttcctttttc
     3541 caatccgcgt tttcaattgc cggttatgga tgcgcgcaca tctgttcagg ggattttaca
     3601 atccgatatt agtggaacgg tttcaatgaa tttaattaaa ttgggttcag atatagcggt
     3661 aggagataca attgtaactt ccaatctttc acggctttat ccaaaatcct atcctgtagg
     3721 aaccataaaa aggattagag aatctcagga taatttgttt atcagtgcag atataaatcc
     3781 tttcacttta gtggaaaatc ttgagcatat atttattcta aaaaggcagg aacaaattga
     3841 gctatagaaa aaaaacagaa ccggtcgtca cccattgggg agtgcgattt gaacgcattc
     3901 cggtgaaaac gaaaatcctt accccccaag ataagatcag tgatataatt aaagaatacg
     3961 ctttaccatt aatgaaacca ggtgatattc taacaattag tgaaagtcct ttagccatca
     4021 ctcagggaag ggcaattcct gtttctgaaa taagagtttc tcctttggca aaactactta
     4081 gtcgctatgt agctaaagtc ccatacggta ttggtttagg cgctccaacc agtatgcaat
     4141 gcgctattga agaaacaggg attatgcgta ttctttttgc ggctttcatt ggtgccatag
     4201 gaaaacttat aggcaggaaa ggtgattttt atagagtggc aggaatgcaa gctgctttaa
     4261 ttgatgcagc tacaacttca cctgttcctc cttatacaga tacagtaatt aagggaccaa
     4321 aaaatcctca acagatatgt gaggatttac actcagaatt tggctatgaa tttgccgtta
     4381 tggatattaa tgatattggt ggttgttgga tgattggggg gagcaagggt gttagtaaac
     4441 atttcataga aagcgtgatg aaagataatc ctcagggtca gggtgacgaa ttaactccat
     4501 tttgcattgt tcgaagagta gataactaat ggtttggaaa ggaatttgga gttttattct
     4561 tggcctgttt tttctgtatc ttcaaattct ggttatgcct gctttagcat tatttaaagc
     4621 gattcctttc attcttttac cttggatgat ttatattgtt tggactcgtc cgcgtaattt
     4681 tgcactaatt gttgttttta tcatcggaat gatgtttgat acagtaaatc cgatgacttt
     4741 tggcttttat tctcttcttt tttgtttgct ggttttagta atccaggaat ttcgtaagcc
     4801 ctttgaggca gaaagcggtg tagccaaatt gttggcaatt ggaattgcta atttcatctt
     4861 ttctgttttg caactggtta tgtatggagt tacatttggt tttgatgggg aacttttggc
     4921 taaatcatta gtcggttttg gctataatgt tatagtatcg attgtcgttt tttggagtat
     4981 gcaactaatt agtaaagtaa gattaacgat taacaatgac taaaaatcaa gctaccataa
     5041 cctattcgat cactttggcg gttctgttta ttttacttgc tgcttcgctt tttcgtttac
     5101 aggtagtgga aggagctaaa tatcaacgca tagcggaaag taactttgtg cgcattcgga
     5161 gaataatagc cacgcgcgga gaaatttatg atcataaata caggccaatt gttcaaaatg
     5221 tcccttcgca taatatgtat ttaaccagcg ggaaaattaa tgatataaat gctctaagca
     5281 gatttctgga acagaatttc ggcattacgg aaagcgaatt gaaagacctg gtattgaaac
     5341 aacgttttaa aacttatgaa gaaattctgc tggcagataa tgttccctac gaaacagtat
     5401 tagctcttag cgaagaattg aattattatc ctgagctttc tttccatatc ggttccaccc
     5461 gtaattatct atatccaaat cattttagcg gttatgtagg gagaattaac gaaaaagaat
     5521 atgacctgta taaaaaagag gattattctc tgaatagcta tatcgggaaa acgggtttgg
     5581 aacgctatta tgaagtttta ctacgcggaa aagatggccg agaaattgtt caggtggatg
     5641 ctcaagggca cagtctacaa ctttttgaag aaggcggaaa tattgaaccg cttaatggac
     5701 tttcccttgt tcttactata gataatgatc tgcaggaatt tgtctctgga atttttccct
     5761 ctggaatgaa aggagctata gtagttagcg atgttcgcag tggaggcatt cttgcttatg
     5821 taagtaagcc agattttgat cctaatctct ttatgcaaaa aataacacca gaaatatggg
     5881 aagatctgaa tcgcgatgaa aagccaatgg tagatagagt tatctacgga acctatcctc
     5941 ccggttcagt ttttaaacct attactgcag gtattggttt agaccgcggt ttaattactc
     6001 ctgaaacaca cctggctttt tgttcgggag gttttaaagt tggtaatcgt tttttccgat
     6061 gctggtttag cagtggacat ggttctacta cagttgttga tgctttaaga gtttcttgtg
     6121 atactttctt ttatgatctt tccttaaaga taaaattgga tgacttttat aattatgtac
     6181 gggattcaca tctatttgga aaaacgggaa ttgacctacc aaacgaaaga cagggcttct
     6241 ttcctaatac agaatggtat aaaaaaacct acggcaaaaa tgtaggaatt attggacaca
     6301 aagtaaatct ggctattgga caaggagaag tattaactac ccccttacag ataaatgcct
     6361 tttatggagc tcttgccaat aatggtttat ggattcaacc tcatttattg aaacaaacag
     6421 ttggtagagg acgtcttact cgtgaacagg ttgtacctat agctaaaaga agattaccta
     6481 tgaaaccaag taccctaaaa actattcaac agggattatg ggctgtagtt aatgctccag
     6541 gtggaacagg agcaaatgca aaaataaacg gagctactgt ttatggtaaa accggttctg
     6601 ccgaaaatgt tatgggtaga aaaactcatg cctggttctg cggttattta gttacttcca
     6661 aaccagaaat tgcagtaact gtttttctgg aaaatgctgg agggggagga gctatggctg
     6721 ctcctataac tgctaaaata ttcaattatt atttgggtaa tttagatatt attaaacagg
     6781 taactccctt gccaccacag tttagaacag cggaaggcat tgagaatgaa gaatttgaac
     6841 ctggtttaga gggctcggaa gtatctatcc ctggaagtga aattgaacct ctaccggctt
     6901 cattgcagga agaaagtgat gttcattaac cgaaaaaagt tcgattttgt cctcttgacg
     6961 cttttattgc tgctgattat ttttggctgt attgtaattt tcagtgcttc cacaactgtg
     7021 attgcgggag aggcacacac tcaaaataat tggtggaagc aggttatctt tgctgtcctt
     7081 tccttattgg ccataattct attgttatgg attccaatgc cgatttttga tttagtagta
     7141 ttaccccttt accttttgaa tatactggct ttgatcatag tgttatttac tcccataatt
     7201 aatggagctc atcgttggtt ttctgtagga ggaataaatc tccagccctc ggaaagtgct
     7261 aaattgctaa caattttgat ggtagcgcgg gttatatcta aagaaaattt gaatgaatat
     7321 cggcaaattc tctatggatt tttattaacc ctgatacctg taatcttgat tatgattgaa
     7381 ccagattttg gtacaacgct ggttttttgg gcttctctga tcgctatgtt gattgctgct
     7441 gatgtacctt tattttatat tttgctatgc ataagtccta tagtaagtat aataagttcc
     7501 atctggtggc cagctattcc gatctggatt ataatttttg tattattact tttgaaagcg
     7561 cgtctttctt gggttacaat tacggtggca agtattatca atgtatttat agcattaatt
     7621 actcctgttt tttggatggg attaaaggac taccaacaaa atcggatttt aacttttatg
     7681 gatcctactc gtgatccttt gggtgcaggt taccaaatta ttcaagctaa aattgctatc
     7741 ggtagcggtt ctctgtttgg caagggctgg ctgaaaggaa ctcaaaaaaa tatgaatttc
     7801 ttgccggaac accatacgga ttttattttt tctgtagtgg gtgaagaatt cggttttttg
     7861 ggctctattc ttctattaat tttgtttgcg gcattcttca gcagaattat taataatgta
     7921 ggtgaactaa aaattcgcga aagaaaagtt gcttcagcag gcattttggc ttatcttatg
     7981 tttcagacct ttataaatat tggaatgaat attggtttag tcccagcaac gggaattccg
     8041 ttacccttaa ttagttacgg tggttcgaat ctattgatta atagcatcgc cgtaggagtt
     8101 gttctaaaat atatgaacga aagaggtttt atgaaatgaa gaatcgcgtg cttggtcttg
     8161 tcttattggc attactatcg ttttttataa atgcctgttc agttgtaacc accctcagtt
     8221 ttacagaacg agatattaaa gatagcccca atctggtaga aaattatagt tttagtccct
     8281 attccacgat tggagaagag gctttaaagg gttggatggt aattcttaat cctcatgata
     8341 attcagaaag ccctattact attgatccag atactgcttg tgaagggaaa acttctttac
     8401 gaatagatgc ttctgataaa tctgtgctta tcttgagtga gccatttaat gtccgacgct
     8461 atggaggata ttatttaaga tgctatttta aaacaaattc tcctaatcca ccccgtcctc
     8521 aactaaggtt tattgtcttt aaggaaaacg gtaaaattgt taatcgcttc aaaacaaaag
     8581 ccaaaattaa taacgattgg caaagaacaa cgattagcgc tggtttcatt aaaccaggtg
     8641 ccagatttgg acgcctgggt atttatattc caccttttaa agagggctct atctggatag
     8701 atgataccag ctgctttgaa gtgcatggat tcaaaatcga ctgatgcgta ttactttgca
     8761 aagggttagc aaagcagttt gctatgtgga tgataaaata accggacaaa ttgaaaccgg
     8821 acttcttatt tttgtggggt tcagctcttt tgatactgaa gctgttttac ctttagcggt
     8881 tcgtaaatgt ctggaactgc gtatctttga agacgaaaat ggcaaaatga ataaaagtgt
     8941 ctccgatatc aatggttcgg tgttagttat ctctcaattt accctctatg ccaattgtca
     9001 aagaggaaga agaccagatt ttactcaagc tgcgcctccg ggaaaagcaa aagagcttta
     9061 tgaggaattt gttcatctat ttaaacagac ggaaatcaat gtccaaacag gtatctttgc
     9121 tgccaaaatg caaatagaac tcgtaaatga aggcccagtt acaatcaact tagagttttg
     9181 atgtttaatt taaaaaagag agattctagt agaacacaat taaattgatt ttaatgaatt
     9241 aacacagcaa tgtaggcaag cggcactgag gatagcgttt tttcgttata gtgataggga
     9301 acaaaaaaca ctatgaaaat atctgtctgt atgtttctca taatagaaaa aaatcgccaa
     9361 aataaaaaaa gccgtttctc cccaaatgaa acggctataa actgatcaaa agatagcttt
     9421 tttatctatc atttccgttt atgacgattc ttacgcagac gctttttcct cttatgggta
     9481 gcaattttat gacgttttct cttttttccg caaggcataa tcttctctct tattactttg
     9541 tttttacatc aaggacagaa tttttgaatt cgcgggagaa tttaaaagta ggaacggttc
     9601 tttcaggaac cggaactttt tcgtcagtct tggggttgcg tccaacacga ggtttacggg
     9661 ttttgagttt atatgttcca aaaccccgga tttcgatatg attaccttta attaagctat
     9721 ctttaatggc ttgtaaaaag gcatcaacaa caacagcaac atctttacgg ataattccgg
     9781 tgttttccga aataatcttt actaaatcgg cttttgtcat ttttccatct ccttaaatat
     9841 acttatcgtc aatattattc attgctatat cgcaataagg tattcataga taaaacctta
     9901 tgatttatgt caagagaaat atctaaaaat atgcaatgag tagtgctgat attcctttta
     9961 caataaagca ttaataggtt ttaatgaaat cacatttttt gtgttcccct ttggtaaatt
    10021 atcctttaaa cacgctaaac caaatagcaa attaaggatt ataggatttt tcgaacagac
    10081 ccagaactat cgcagaatga attggttaca tcattgaatt aggcatcatc ccttaacccc
    10141 aattttatta acaaagaaag ggacaaaata tgaaataagg tgtggcagaa acagatagaa
    10201 ttaccacatc gcaagttaag gaaatgggta cgagtagaga ataattagca acactaatat
    10261 ttctcttgac atattaagcg gtactgaaat tatgaagctg gattgtgaat tatgaaaaaa
    10321 taacttgatc aataaataaa aagtaggtga gatatggaaa cagccatttt aagtcccctc
    10381 tctaatatgt gtaccttcca ctgtaaggga aaattagttt ttctctccta cggtggaaga
    10441 gagcataagc ttttctatgg taaaggaatg cagtatctta taactttaat tgcgaatccg
    10501 gatttgtata tttcttgtgc taatattgaa actataaaac caatgcctca aagggtatac
    10561 agtcagtttt cctcatctaa tgaagtgcaa attcatcaat tggaggttca gagtagtttt
    10621 ccctctattc ctttaaccga tttacaaacg gttaaagaag taaagcaaga attaattaga
    10681 gtgatagaca cacttgctga attggaggca aattgtgatt atgcacaagc tgatgatatc
    10741 agagatcaat atgagaaact tgttcaatat ttggaacaag tttataagcc cgaaggtaaa
    10801 attcgttatt ttcccagcga agggaaaaaa acaaaaaaga gagttttacg tgctataaac
    10861 cgttccttgg aagatataga aaaattggaa ccagagcttg ctgtagagct aaagaactcc
    10921 ttggaattgg gaactatttt acattatcat cctaaaatag atatagaggt tttaggatat
    10981 taaacaatag ttcttgtttt caagctttat tacttattta aaatgtgctg cacataaaag
    11041 ggttgtgtct caaggttggt gtaaaattgg ggaagccaga ttaagaaaaa agtagagccg
    11101 gattccgctc tttgttttga tggttttgct aaaactaaaa agacaaggat aagatcataa
    11161 cccaacccaa gagaaagaag gatgaagata atataattgc aagtatatgc taacatagat
    11221 tgtgagaaat gagagtagta aaatgttttc caacaacccg atatatagcg agttggaatt
    11281 taccccaaca ttggggactc aactattgtg agcatagcat taaagcaaag ttcttatctg
    11341 gaatgaatat tgcttttaga attgaattat gaaaaaaaat atcttaattc ttatctgtct
    11401 tatactgatc agccagtttt ttgcaatggc aaagcaaaat gaggatagag agcgtcctct
    11461 ttatgctaat aaccttgtaa agattaagct aagttcagaa gcagtatttc gtgcccagtt
    11521 gccgttatca gcttatgctg aatgtgaaaa aacaaacctc aatgaattag atcagctttt
    11581 tgaggtgaat ggcgttcaat caattatccg cgctcatatt tctgccaaag atagaaattg
    11641 ggaaactaaa acgggatttg accgctggtt tttactgcgt ttagatggca caaaaacagt
    11701 tgaacaagta atagctaatc taaaaagcaa tcgctatata gaagatatta ttcccgaata
    11761 ttatgcctat ctgcataatg ttcccgatga tcctctatat cccaataatt ggggacacaa
    11821 taacactgct cagctacctg tttatcaagg tgattctcat agtgggcctg gagtaggggt
    11881 tataggattt gatagcgatg cagaaattgc ctgggatagt aatcaaggtt atggagatcc
    11941 tgatattatt attgggattc ttgatacagg tgtagatcta aatcatccag atttactttt
    12001 agtttccgga tatgatttcg gcgataatga tgataatcca atggatgata gtgccgaagc
    12061 aggtcatgga acatgcactt ctggaattgc agcagctaaa gcaaataaca ttttaggggt
    12121 ggcaggaatt gcaggtggct gtagtgttat gccgctaaaa gtagccagca gtaatggaga
    12181 tatactatat tcagctatta ccaatgctct tatttatgca gcagataata atgtaaatgt
    12241 agtcagtatg agttttggtt caaattctca tgtaggggat aatcctactg ttgattcagc
    12301 tttagaatat gcttataata atggagttat tctctttgcc agtaccggaa atgcaaatag
    12361 cgctacaata ggttatccag ctatacatcc tttagtaatt tcagttggcg cagcaagtcc
    12421 ttctggagaa agaaaaaatc ccaattcctc agatggagaa aattggtggg gttcaaatta
    12481 tggaaccaat caacaagatg ctgcaggtgc agttgatata atggcaccta caattcttcc
    12541 tgcaacagat attacttcct ttggaggtta ttctctagat gactatttta tgtggtttaa
    12601 tggaacatcc tgttcttgtc cttatgcagc aggagtagca gctttagttc tttctgtaaa
    12661 tccaactctt actccggcag aacttagaac cataataact tcttcagcca cagatatgac
    12721 agcaggaaca tctgaaggtt gggatcgatg gactggttat ggaatggtaa atgttgctca
    12781 ggcactttgt gaagccaatc caagtattcc tctctgccat attgtttacc cgcttaataa
    12841 tactggtttt gaaatcaatt ctgttattac tattcaggtt gaagcatctg atccggttga
    12901 gcgcagtatt ctggaagttc gtttttatct tgatgacaat ccagattatg ttttttctga
    12961 ttcagaaatg ccttatgaat gggtatggaa tacagcaggt ttatctatag ggcagcatac
    13021 aataaaagct gaagctattg ataatgaagg aaatcggcga tatcattcta tatccatatc
    13081 actcttaaat cctgctgatg aaggattcga aacagccgat ttttccaagt tgatttggct
    13141 tatcaaaagt tcttcgcctt ggactgtgca gagcgattca tattttactg gagaatattc
    13201 agcttgttct ggtctaataa atgcagaagg agcttcagag ctgaaattga aagtgaacat
    13261 aaccgaagct ggagaaatca gtttttaccg gaaggtttct tctttggcaa attacggtta
    13321 tttgaaattc tatattgatg atgaattaat tggagaatgg agtggagaaa aggactgggc
    13381 ttgtgtttcg tttcctgtaa atcctggtct acgaacattt tcctgggcgt atcaaaaaaa
    13441 ttccagtaat gtatcgggga gtgattgttg ttggatagat catataatta tgccccccta
    13501 tgaaaattac tattggcctc ctcaaaatct aactgctgca ggcggatatg gatttgtgtt
    13561 actatattgg aaagtacctg aagcgggtga acctactggc tataatattt taaagaataa
    13621 tgtgttgctt actaccattg cagaaaccag atatacggat actgatgttg tagctggcat
    13681 ttcatatgaa tatacagttg tggcagttta tgccgaaggt caatctgaat caacatctgc
    13741 tcaaactgct attccaactt ctgaagtagt taccgaaacc attatcggaa acggaatcat
    13801 cccaacttct aactatgaag gatgtcctat caataattat cgtaaaagtt tacacggtca
    13861 gatgatctat accgctcggg agcttaatct acacggtatt ttcggtccta caattatcac
    13921 tcgtttagga ttttatataa acacagcttc agcatattca cttcccaatt tcagaattcg
    13981 gatggcacat actaccgcag aagatgtaag ccaatggctt ggcgaagatg atcttataac
    14041 ttgttatctg gcaactaatt atcgaccagt agctggtgat tatgatatgc tcactttctc
    14101 aactcccttt ttatggaatg ggatagataa tatattagtg gatactgctt tcagtttaac
    14161 tgtggctgta aaatcgtctg gaacagtact ttatacggat acacctaaag gctatcatta
    14221 cacacgcagt aatgtagagg atcaaactga tgtttttact ggaggtgctg taactatgta
    14281 taaaccaaat atgaaattat cagttcttac tcaaccagat attgcagtga acaccaccaa
    14341 tctttctttt ggaaatgtgc ttgctggtta tacagcaggt aaaacattta atataactaa
    14401 taccggaaca ggttatttga atggtgaaat tacagttcct gcaggttata aaattgtatc
    14461 cttggatgaa gagtatagtt ctttatcata ttctattaga gctgatagca cggctgtgtt
    14521 tactgtcctt tttgcacctt tagcaccggg tgtttataat ggaattatat ctattaccca
    14581 caatggggaa ggggaagaga gaattataaa cttaagtgca gtttctctta ctgcgctaaa
    14641 tactcctttt attgagggct tcgaaaatta ttgtgaaaat tggacttttg taaacgaagg
    14701 tcaaacgaat aaatggtttt atgggtttat tgttcctcaa tcgggaaatg gaggactatt
    14761 tgtaagtggt gataatggac tcactaattc ttatgttcct aatagaggct ctacaagtta
    14821 tatatacaga gatatttatc ttccccaagg aaccgagaat tgtaaattgc gtttttcctg
    14881 gaaaggaatg ggggaaggaa caaatcccta ttttgatttt ctcagggttt tttgtgtaga
    14941 acccaatttc gtacctcaag caggaactat gataagcagt ggacagcttg gcacaacctt
    15001 aaatctttcc gatcattgga aagaagccga ttttaatatt cctgaaccgg tttctggtgt
    15061 atataaaaga atcgtttttg cctggagaaa tgatgcttcg ggtgaaacgc aacctcctgc
    15121 tgcaattgat aatgttcgcg ttatattaaa tagcaatacc gattatgctt atgtttatga
    15181 tggatcagcc actgtaaatt tacctgcggt ttcggacacc ttaggccata cctatcacgg
    15241 aaatctaatt attactggaa ttacagaacc tgatgaatat ttggtagtta cagctggctt
    15301 tgcttctttg aatgaacctt tttctgatgc cggaatggat tttactgtta caggagccaa
    15361 ttttagtgga gccgaaatta cttttaacca taatttaggt tatgttcctt attaccttgg
    15421 ttataaaatt gaaggaagag gagagtggat gaccttttcc gctgctccaa attggacaga
    15481 taaaattgct tactttacag ttcccgtcta ctatggaaat gccacgaata tatctttctg
    15541 ctttcctcat gcgaataatg tatctccata ctatccagaa ttgaatgtag tgctcgatat
    15601 gcaaaatgta gttagcataa tatggaaatg taactataca gaaggaattt ctgcttatca
    15661 aatatggagg ggaagcagcc cttctttcaa ttcagcagtt gcagtaagtg atctaatccc
    15721 tcatcttgat acccaagaag aaaattatgt ctataccgat actacaactg atgaaaatac
    15781 gcgttatttt tactggctaa gaattatcaa tatagatgag tcttctagtt attggggacc
    15841 aaaactaatt gttactggaa cagatccatt acaaccacct ccggaaacta ctcttttggg
    15901 agcgtaccct aatccattta gatatggaac caatatcagc tacactatca aaaatgctgg
    15961 aaatgtggaa tttaaaatct ataaccttaa aggtcaattg gtaaacaact atctccaata
    16021 ccataataat tatggaactt acatttatga atataccggt acagacaaaa aaggcaagga
    16081 actagccagt ggactatata tctgctatat gaaatttggg aataagacct ttgttcagaa
    16141 aataataaag gctaattaag ttaactgcaa aaaatataaa cggttttatc gatataaaag
    16201 ttgaagcgtt aaatggttgt ttaacctagt gatcgctgtt gtgtaaatca gaagttcaaa
    16261 gttcaaggta ctggagttca aatctgtcgt tcctccgaaa aaaatattct ctcacagatt
    16321 acacagatta cacagatata gtttttgga
//
LOCUS       DAORHB010000006        15799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_2806_length_15799_cov_4.274327, whole genome shotgun sequence.
ACCESSION   DAORHB010000006 DAORHB010000000
VERSION     DAORHB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2806_length_15799_cov_4.274327"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKM80_00380"
     CDS             complement(<1..386)
                     /locus_tag="PKM80_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNV92392.1"
                     /translation="MPFVHLHNHTQYSMLDGACRVDRMIKLAKKYEMPAVAITDHGNL
                     FGVVDFYKTARAAGIKPIIGIEAYIVNGDIESESSKNESRYHIILLAQNEQGYKNLVK
                     LSSISYIKGFYYKPRISKTLLQQYNEG"
     gene            complement(937..1275)
                     /locus_tag="PKM80_00385"
     CDS             complement(937..1275)
                     /locus_tag="PKM80_00385"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HNV92393.1"
                     /translation="MSDTEKTKCNHNGDHICDEECECNDNFITLEMDDGTLKNFFIIG
                     NLENEGKYYIALVAEGESNYDILQVTEEGDNVNLNYIDDDNEYNTIADKFDELYQKEL
                     KEMGIEEETE"
     gene            2153..3232
                     /gene="buk"
                     /locus_tag="PKM80_00390"
     CDS             2153..3232
                     /gene="buk"
                     /locus_tag="PKM80_00390"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNV92394.1"
                     /translation="MSYRVLAVNPGSTSTKIAVYDDSTPVFEKTLRHDPEDLNKYGDL
                     FNQTDFRKTLVMEAMEENNVHSQTLNAVVGRGGLVRPVSGGTWKVNDSMLRDLQDSTI
                     WGRVHASNLGAFIAKAIAEPLGIPYFIVDPVATDEMEDIARLSGIPEIDHKSLFHALN
                     IRYICRLLAAELGKKLEDVNLIGVHLGGGISVAAIKKGRVVDVNNALLGTGPFSPQRA
                     GSLPIGDLIDMCYSGQYSKKELTAYLSKGAGLMAYLGTDSGIEVAQRVSEGDQKAKNV
                     LDAMCYKVSKEIGCCAAVLSGKVDGIYLSGGLAYNDYIVEFIKQHTEFIAPLYVYPGE
                     KEMEALCQGGIRVLNGSEEAKEYPY"
     gene            3229..4905
                     /locus_tag="PKM80_00395"
     CDS             3229..4905
                     /locus_tag="PKM80_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNV92395.1"
                     /translation="MKYVLIILLLSIGLVLNAESWTVLVYMAADNNLAEMGRLDINSM
                     ESVPQPEGLNLIVQADFPEGAKRFRIQQDNSEDISSPVLANLGIIDSANPEVLNSFIN
                     WGFNNFPSQRKMLIIWSHGDSWYKGNDPKWICPDESSQNLISVAEGELTLALTDIPKL
                     DVLLFDACSMQSIEVINEIGFCANYIIGSEDLVPQYGFPYEKIIPLLEGDLDILIAQI
                     PDIYVSSYLPGQGINPGGNLWPITCSVIKTEMLPLFVQKFKSFAISQRPLASKYLPIR
                     HSCYSMNTGLADIDIRDFLEKAQTICDSEAQNLLNLWNTMVISSSFTNPDYTGNIGTA
                     AFWFPESRFNFENGWQRYFNLDFAKTRWLSFVNAALGNDNIPPQPPILNSQNLIYNTL
                     QLSFLASPDPDSLYYEISYDGFEHFQYIYPNPDNSIFNVMLQISKSGTYQLRAIDQNG
                     NKSEPVIGSYDYSTPNVSMLVNPNPISGTSLAVLRWWAGDELSGKIQLEIYNLKGQKV
                     LSKYLGEVYPGEGNFLLSSDQNFRALPSGVYFLKLQLGKRKLTKKMTILY"
     gene            5081..5818
                     /locus_tag="PKM80_00400"
     CDS             5081..5818
                     /locus_tag="PKM80_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV92396.1"
                     /translation="MINNIAYPSFSAEHLRYLSKLKQKKYRLQEKLVIIEGKRTLEQL
                     FSWGIKAIELYVTNEEPTITAEKIYTISEQGLLRICDSEHPQKIAGLFPLPVARKIAF
                     RKAFYLDGISDPGNLGTIFRIAAAFNIDCLILSPNTCEVSSPKVIRASLGAVYKVPFF
                     NCAAENLKELNANIFALDMQGKIPLANFEQPSEPYIVALGNETKGLSSSILEIATETL
                     CIPMLGEMESLNVSISAAILAYTISEK"
     gene            5883..6446
                     /locus_tag="PKM80_00405"
     CDS             5883..6446
                     /locus_tag="PKM80_00405"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNV92397.1"
                     /translation="MLNIVNQKTGWIEVICGSMFSGKTEELIRRIRRAEYAKQKVQVF
                     KPIIDNRYDANNIVSHSQMQAASIPINSPGEIYSYLDDDVKIIAIDEAQFFDSSIVGI
                     CNDLADRGYRVIVAGLDQDYKGEPFGSMPQLMAIAEYITKNLAICVICGNPANRTQRT
                     VHQDEQILVGSTDAYEARCRNCHEVLD"
     gene            complement(6574..6711)
                     /locus_tag="PKM80_00410"
     CDS             complement(6574..6711)
                     /locus_tag="PKM80_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92398.1"
                     /translation="MSELISFVNKLRIKKAIMGEKSKPIIGGKIRRIGPKSGSVIVNK
                     K"
     gene            6730..7314
                     /locus_tag="PKM80_00415"
     CDS             6730..7314
                     /locus_tag="PKM80_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNV92399.1"
                     /translation="MPLFPLDIRHQEFSSSMFGYSKKEVRAFLDQVASDVETYQKQLQ
                     KELARQEQIQNEVKQEAVQAGSAVEELKRREELISRTLVFAEKTKADIIANARKEAEN
                     IIKEADLKAKRAIQEAKQYLSVLEHQYLQLKEQKRQFLIQFKVELQSFQDRISKDPLL
                     SKDSELMLDSEFHQIKDDFVPKPENNEPQNTKSQ"
     gene            7315..8145
                     /locus_tag="PKM80_00420"
     CDS             7315..8145
                     /locus_tag="PKM80_00420"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNV92400.1"
                     /translation="MNNYQETAIWLKNKFSEKPLIAVILGTGLSDLPEAFPVLCSLPY
                     NQIPGFVKTTSPSHPGNLLLCEVKGIPIIFLQGRFHYYEGYSMQEVVFPVRVLASLGI
                     KVLIVTNASGSLRKELKPGAIVQLQDHINFMGTNPLIGINDDNLGERFPSLNNPYDPE
                     LCSLADEVAKELEISTFKGTYIAVTGPSLETKAECNAFAILGADLVGMSTVPEVITAR
                     HSGIKVLAYSIVTNYSNLFHNLAHSQEEIQKTALNSGRQLQKIIAEFIFRVTKTFSLT
                     "
     gene            8208..8609
                     /locus_tag="PKM80_00425"
     CDS             8208..8609
                     /locus_tag="PKM80_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNV92401.1"
                     /translation="MAAKKLSPDKLRHFEELIRGELRESLDYINNINKEQSLGARESS
                     GDLSSYAYHQADQGSDTNLMEQTVMMLESEREKIRQLNEALRRIADETYGVCEICGEF
                     IQETRLEIIPYATCCVACQEKSEDKKRRQKR"
     gene            8606..9172
                     /gene="lspA"
                     /locus_tag="PKM80_00430"
     CDS             8606..9172
                     /gene="lspA"
                     /locus_tag="PKM80_00430"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNV92402.1"
                     /translation="MKPPLTKLKTAPAYFLMMLIVVMDQLTKTLVRVNMKLYEHIPLL
                     EGLFGDTFLLIKVENPGAAFSIGLGNDLVNRIFFISITVIVVGLIIYLLRRAQHQIQV
                     IAFGLILGGAIGNLIDRVLFGPVTDFFSMDFPDFIMERFPVFNIADSAIFIGVCLLII
                     DMLFIKDNSSDSVEEDEQLIINESDKEL"
     gene            9173..10606
                     /locus_tag="PKM80_00435"
     CDS             9173..10606
                     /locus_tag="PKM80_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92403.1"
                     /translation="MKNYLLTFFALVFITGISAQAVLTCYDIQYTTDPTGNSPYYNQT
                     VTVQGIVTHIIPNSAFYIADPEGGEWSGLYVYHRNTSNVVSVGDLVKLTGTVDEYFNL
                     TEFTNVSAYEIISQGNALPPFLELSTANMPSGTNHPENEKYEGVLVRFQDVTIKSSPD
                     GYGQFNIADSSNIWAMVDNGLYAIPANSIVIGESWYIIQGIVDFHSSAGFKLNPRNAN
                     DMIKQDTIENSIIKIVRTDPTTDPLINTDVSMNLISTKIKAEWGVQSYSVSFKLNPAC
                     IHFSGFDTEGTLTPSIPDYYISSAGDTISYTVTYQDGLIYNQNDALLIKILVKPLVYG
                     ESVIELVSFKYNDTNVLNLNDGSLVTKLQKKTAYLDISKIGDKKNIFNPEMGEQIIIS
                     YGTKTGYLSKAIVRIYDAQGRLVYTPVHTNITSSTGIENFIWNGRDANLKLLPPGLYY
                     CHLEVIDRSSGDKDNTVQPIVIKSALK"
     gene            10615..11460
                     /locus_tag="PKM80_00440"
     CDS             10615..11460
                     /locus_tag="PKM80_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92404.1"
                     /translation="MKKLLFACLLLIPATMLLAVYDDYQPSAIARGLGGAYTAVSRDA
                     NAIFYNPAGLVNTTLSAKLGFSQLYNQDFSELKTVALGYKLPQKLGTVALGIRAMDVD
                     YEDVNLMSEQVYSLAHSFYLLQDIHSQISFGYTGNFYLLSMDGEDDDTALGLDLGAMA
                     VLHTRTRLAFAVTNINKAKMGDENQIDLPSKLALGISYIPYDKVTTSIEVKKDFAKET
                     EFMGGVEAHLFEPLAIRFGVHQNPATYNAGLSIYIRDLELDYSYTYHSVLPGTHYFSL
                     GYNFK"
     gene            11471..11749
                     /locus_tag="PKM80_00445"
     CDS             11471..11749
                     /locus_tag="PKM80_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92405.1"
                     /translation="MGMKFKTMIPTFIPIVMEMMRVFKRNSKHDQDIKKKDRTAEKLG
                     SLEHLMVRLEKQVQHNRELYTKSLNKIRIWLCLNSILLIAILIKLFLG"
     gene            12056..12132
                     /locus_tag="PKM80_00450"
     tRNA            12056..12132
                     /locus_tag="PKM80_00450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12090..12092,aa:Arg,seq:acg)
     gene            12178..14094
                     /locus_tag="PKM80_00455"
     CDS             12178..14094
                     /locus_tag="PKM80_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92406.1"
                     /translation="MAKQRKNQKRWNNIILIFRMMLRYWHYLVAGFICMLFFTLFNGV
                     SVTLAIPLFDYVFKPGRNNLLYTDFSGFLTALGELIKQHFGSSGGFFAAIQNYNSLWE
                     GCKKLMLQTDSLSLLYGLCVAIFVIILLKNGFYLLHKIFFNSLRGKTIRDIRNYMFTR
                     YLNQSLTFFGRHRVGDAIVRMVGDVDIVSEQLINSFVNVTREIFTVLVFARIAYLLNP
                     TLLLYSIIVLPVFSFSIGLLGKKIKKYSKRIQEQISNLFSNVEEVLNSMKVVQAFRRE
                     DYEKKIFESINQKHYRMWYKAQYYGALNTPIAELNTAITGILVIIIGGNMILAPGSNF
                     SLGDFTAFLFALFSMLHPLKVITQVYTDIRKALVSLDRIGLVLNQESRIQDKPDAIVK
                     DSFDREISFDNVTFGYKNTKPVLKNFNLTISKGSKVAFVGSSGGGKTTIANLMNRLYD
                     VNSGAITIDGIDIRDIKLDSLRKLFGVVTQESILFSRSIKENIAYGSQDEVTDEQIIK
                     AAQIAYADEFIDELPNKYEEVLSSKGSDLSGGQKQRLCIARAIVGDPPILIFDEATSA
                     LDTDSEQKVQLAIDKVTLNRTVILIAHRLSTILKADQIVVLEKGKIVGIGTHQELLES
                     CSRYQHLYNLQNGK"
     gene            14129..15073
                     /locus_tag="PKM80_00460"
     CDS             14129..15073
                     /locus_tag="PKM80_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92407.1"
                     /translation="MKISGFSFVRNGIKLYYPVIESIQSILPICDEFIIAVGKGDADD
                     DTKERILALNNPKIKIIDTIWDEKLCKRGMINSWQTDIAKKECTGDWLFYLQADEVVH
                     EKYLPVIQKRCEELLNDKEVEGLLFAYKHFWGDYYHYHNGHGWYPYEIRIIRNNPNIH
                     SYQSAQSFRYFEYYDNPRQETGTRKLKVAKVDAEIYHYGWVRPPNLMQNKCKALNSVH
                     WGKEKAEEYYNKAPKYFDYGPLSQLAFFVGTHPIVMQNMITNFNWQDKLQLTGKPNPY
                     RELHKHEEFKYRFLTFIEKHFNGGKQIGTFKNYVLLKR"
     gene            15070..>15799
                     /locus_tag="PKM80_00465"
     CDS             15070..>15799
                     /locus_tag="PKM80_00465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV92408.1"
                     /translation="MNLSVIIPVYNYLDLLEKCLFSVYRQAVQPDEIILSDDGSEEDI
                     IGFFQVQKEKTSIPMILVRQEHQQFRAARVRNNGVSVCNSEILAFLDQDIIIPKNYLL
                     SIKNELTIGRFLSGYPVRLSKDQTEKLTFEVIEQNNWNAILTEAQKKKIIKQYRKDYL
                     SYVLCKYLPFVEHGAKMRGGVSAIFRNDFEAVNGYDENYIGWGNEDDDLGKRLHALGR
                     IGFNFVLSQLPLHQYHQPYHNKGER"
BASE COUNT         5058 a         2608 c         3017 g         5116 t
ORIGIN      
        1 ccttcattgt attgctgcaa aagagttttg gaaattctcg gtttataata aaatcctttt
       61 atataggaaa tagaagagag ttttactagg tttttgtagc cctgctcatt ttgtgccagc
      121 aaaattatat gatagcgact ttcattcttg ctgctttcac tttcaatatc cccatttact
      181 atatatgctt caatgccaat tataggttta atccctgcag cacgggcagt tttataaaaa
      241 tccacaacac cgaataaatt cccgtgatct gtaattgcca ctgcaggcat ttcatatttt
      301 tttgccagtt ttatcatcct gtcaaccctg caggctccat ccaacatact atattgggta
      361 tgattatgta aatgtacaaa aggcatctct cactcccttt ttttatctaa gtaagcaaaa
      421 atgaaatagg gaaatctgtc aaagaatatc tatatcttac aacagaaatc agaagtcccg
      481 aatttgataa tttccaacac ctcatttgat taatgaatca gtaataacac atagcgaaat
      541 tagccttttt tcccaaaaaa tgttcttcat aaaaaagcgg actattgaag gaatcgttaa
      601 cttattttaa gacatttttt agcaataaaa taggtatttt aaaagagaag tttgattctc
      661 gataacttct ttcacttgct gttaccagaa atgttttttt gctcttgtta ttataaacca
      721 atcaaagtag ttcattgata atatatagtt gctcctcctt gctaaaaagc gtcatttaat
      781 tataatatac gattagtttg gcttctcttt tcccttctat gaaaaaggga tagcaaatat
      841 cgctacccct tctgttgtaa gataaaattt tggaatagaa ctgttcttta gatagttaag
      901 atcttgtaaa atacagtata actatgccgt agctaattat tctgtctctt cctcaatccc
      961 catttctttt agttcttttt gatacaattc atcgaattta tcggcgatgg tgttatattc
     1021 attgtcatca tcaatatagt tcaaattcac attatcgcct tcttcagtca cttgaaggat
     1081 gtcgtaattg gattcacctt ctgctactaa ggcaatataa tatttacctt cattttccag
     1141 attcccgatg atgaaaaagt ttttcagagt gccatcatcc atctctaaag tgataaaatt
     1201 gtcattgcat tcgcattctt catcacaaat atgatcacca ttgtgattac atttggtttt
     1261 ctctgtatct gacatttagg tctccttgtt taggtttttg tcattgattt ttaatggctc
     1321 tattctggca agctaaatct ttaacttata gtgggaaata aaggtagaga gtatctccct
     1381 tattcataaa ttcaattatt aaggaagaaa taatatcccg tttccataat tcatcaagaa
     1441 ttttcctctg ttaaaaaatc caaaaatgaa agccacatag tttatggtta tatccagcca
     1501 ctgagtcacc aaatttattt ctcctgaatg gtagttaagc attacttcag ttattaacct
     1561 tctgtttatt taccaagata taattggtga gctcttttcc cctaaacaat aaaatctgga
     1621 gttgatatca aatactatcc taatatattt atctgccttc aaatattatc tgccgtactg
     1681 ccaattgcct atcagcttcg atttctacta ctcgttctta agtgaaggcg ttaatcaagt
     1741 gatcatcatc ccttaatcaa gcgttaatta ttaacgcttg attaagggat gttaaacgat
     1801 agattaatgg ttctaagcaa ggaacatagg aaacaacaac atcggtattt tatgataaaa
     1861 gtgattgata aagttggtat aatacagttt caatagaata ttatcgcaat atctttaagt
     1921 tgttacttga atgctatgaa ttgtgattga aacttatagt cgtaatggac ccttatatgt
     1981 atcttaaaga aataagtact ttaggagaaa tactttttgc ctttactccc cagatctttt
     2041 tttatagtgc gaacgatcta atagtaaagc aagccattaa gaaaattgat tgactttttt
     2101 tagctcttta tttttaaagc gagatagatc aaaaattata tctggaggag aaatgagtta
     2161 tcgtgtgctc gcggtaaacc ctggttcaac atcaacgaaa attgctgttt atgatgacag
     2221 cacccctgtt tttgaaaaaa ctttaaggca cgatcctgaa gacttgaaca aatacggaga
     2281 tctttttaat cagacagatt ttcgtaaaac cctggttatg gaagcgatgg aagaaaataa
     2341 tgtccactcc caaaccttaa atgctgttgt aggtagaggt ggattggtgc gtcctgtaag
     2401 cggtggaacc tggaaagtta atgattcaat gttacgagat ttgcaagatt caacaatttg
     2461 gggaagagtg catgcttcca atttaggtgc atttattgct aaggcaattg ccgaacctct
     2521 tggtattcca tactttattg ttgatcctgt agcaactgat gaaatggaag atattgctcg
     2581 cttatcaggt attcctgaaa ttgatcataa aagtttattt cacgctttaa acattcgcta
     2641 tatctgtaga ttacttgctg cagaattagg aaaaaagctg gaagatgtaa atcttatcgg
     2701 agttcattta ggcggaggaa tttccgttgc agccatcaaa aaaggaagag ttgttgatgt
     2761 aaacaatgct cttttaggaa caggaccgtt ttctcctcaa agagctggct cacttcccat
     2821 cggagattta attgatatgt gctattcagg acaatactcc aaaaaagaac ttactgctta
     2881 tctctctaag ggtgcaggac ttatggctta tttaggaacc gatagcggta ttgaagttgc
     2941 ccaaagagtt agtgaaggtg atcaaaaagc aaaaaatgtt ttagatgcaa tgtgctataa
     3001 ggtttccaaa gaaattggtt gttgcgcagc agttctatcc ggaaaagtag atggtatcta
     3061 tcttagtggt ggtttagcat ataacgatta tatcgtagaa tttatcaaac aacatactga
     3121 gtttatcgct cccctctatg tttaccctgg tgaaaaagaa atggaagcac tctgccaagg
     3181 tggaataaga gttttaaatg gaagcgaaga agctaaggaa tatccctatt gaagtatgtt
     3241 ctgattatcc tgctgctttc tattggtctt gttcttaatg cagaaagctg gacggtcctc
     3301 gtctatatgg ctgccgataa taatttggca gaaatgggac ggctggatat taattcaatg
     3361 gaaagcgttc ctcaacctga gggcttaaat cttattgtcc aagctgattt tccagaagga
     3421 gcaaaacgct ttagaataca gcaggataat tcagaagata tttcttcacc agttttggct
     3481 aatttgggaa ttatagacag tgccaatcca gaggtcttga atagttttat aaactggggt
     3541 ttcaacaatt tcccttctca acgaaaaatg ctgataattt ggtcacatgg agatagttgg
     3601 tataaaggaa atgatccaaa atggatttgc cctgatgaaa gctctcaaaa tttgatcagt
     3661 gttgctgaag gtgaacttac tcttgcctta acagatatcc cgaaacttga tgttttactg
     3721 tttgatgcct gtagtatgca gagcattgaa gttatcaatg aaataggttt ttgtgcaaat
     3781 tatattattg gctcggaaga tcttgtcccg caatacggtt ttccttacga aaagatcatt
     3841 cctctcttgg aaggagattt agatattctt atagctcaaa ttcccgatat ctatgtttct
     3901 tcctatctgc ccggtcaagg tataaatccc ggaggaaatc tttggccaat aacttgctct
     3961 gtgataaaga cagaaatgct gcctttgttt gtgcaaaaat ttaagagttt tgccataagc
     4021 caaagacctc ttgcttctaa atatttgcca attcgtcatt cctgctattc aatgaataca
     4081 gggttggcag atatcgatat cagggacttt ttggaaaagg cacaaacgat ttgtgatagc
     4141 gaagcccaaa atttacttaa cctttggaat acaatggtaa ttagcagcag ttttactaat
     4201 ccagattata caggaaatat aggaacggca gctttctggt ttccagaaag cagattcaat
     4261 tttgaaaacg gctggcaacg ctattttaat ttggattttg ccaaaactcg ttggcttagt
     4321 tttgtaaatg ctgctcttgg taatgataat atcccacccc aacctcctat cctaaattcc
     4381 cagaatttga tctataatac tctacagctc tcttttctgg caagccccga tcccgattct
     4441 ctttactatg aaataagtta cgatggattt gaacattttc aatatattta tcctaatccc
     4501 gataactcca tttttaatgt aatgctgcaa atttctaaat ctggaactta tcaattgaga
     4561 gcaattgacc aaaatggaaa taaaagtgaa cccgtaattg gttcttatga ttattctaca
     4621 ccaaatgttt cgatgttggt taaccccaat cctatatccg gaacttcgtt agctgttcta
     4681 agatggtggg caggtgatga actttcagga aaaattcagc tggaaatcta taacctgaaa
     4741 ggacaaaaag tgcttagtaa gtatttggga gaagtttatc ccggagaagg aaatttcctc
     4801 ctctcttctg atcagaattt tagagcttta ccttctgggg tttatttttt aaaattacag
     4861 ctcggaaaga gaaaattaac aaaaaaaatg actatcttgt actaatatta tgggcttgga
     4921 gagaatgtgg ataacctgtg gataagtttt gtggaaatct ctcaacggtt cccatcctct
     4981 ttatatgtca ataagttagg atttaagagt tatccacagg gcttgtggat aagttcctat
     5041 ctgactgata atttagcata ttgcttaaaa gaggtagttg atgataaata atattgcata
     5101 tccttcattt agcgctgaac atttgcgtta cctttctaaa ctgaaacaga agaaatatcg
     5161 cctgcaagaa aaactggtaa taatagaagg gaaaaggact ttggaacaac tcttctcttg
     5221 gggcataaaa gcaatagaat tatatgtgac taatgaagaa cctactataa cagccgaaaa
     5281 aatatatact atttcggaac agggtcttct aaggatatgc gattctgagc atccccaaaa
     5341 aatagccggt ttgtttcctt tacctgttgc aaggaaaatt gcctttcgta aggcatttta
     5401 tcttgatgga ataagcgatc caggaaattt gggaacaatt tttagaatag ctgctgcttt
     5461 taatattgat tgcttgattc tatctccaaa tacctgtgaa gtaagctctc caaaagtaat
     5521 ccgcgcttcc ttgggtgctg tttataaagt tccttttttt aactgtgcag cggagaactt
     5581 gaaagaattg aatgctaaca tctttgcttt agatatgcaa gggaaaatcc cactggcaaa
     5641 ttttgaacaa ccttcagaac catatattgt tgccttgggt aatgaaacta aaggactttc
     5701 ttcttctatt ttggagattg caacagaaac actttgcatt ccaatgctcg gagaaatgga
     5761 atctttaaat gtatctatct ctgctgctat tttagcctat acaatatcag aaaaatgagc
     5821 aattgacaaa aaaagggata tataggatat ggtaattgta gtttttttgg ggaggaaaaa
     5881 gaatgctgaa tatagtaaac cagaaaacag gatggataga agttatctgc ggaagtatgt
     5941 ttagtggaaa aacagaagaa ttgattcgaa gaattcggcg tgctgaatat gctaagcaaa
     6001 aggtgcaggt ttttaagcca attattgata atcgttatga tgctaataac attgtttctc
     6061 attcacaaat gcaagcagct tccattccga taaattctcc tggagaaatt tacagctatt
     6121 tagatgatga tgtaaagatc attgccattg atgaagctca attttttgat tcctccattg
     6181 tcggtatttg taatgattta gcagacagag gatatagagt tattgtagct ggtttggatc
     6241 aagattataa aggtgaacct tttggtagta tgcctcagct aatggctatt gcagaatata
     6301 ttacaaaaaa tttggctatt tgtgtaattt gcggtaatcc tgctaatcgg acccaaagaa
     6361 ctgtccacca ggatgaacaa attttagtgg gttccacaga tgcatacgaa gcgcgttgta
     6421 ggaactgtca tgaggtttta gattgattta tacaacgagt ttaacaggtt ccatcttatt
     6481 atttgttgtc cgtattatac agatatataa catattgatt ttagccagag tttttgcctc
     6541 ctggataatc aggaatccgt ataaccgtct gtatcatttt ttattaacta taacagaacc
     6601 acttttaggt ccgatccgtc gaattttacc gcctatgatg ggtttggatt tttctcccat
     6661 aatagctttt tttattctta atttattaac aaagctgata agctcgctca tataaaggtg
     6721 gaggaattta tgcccctatt cccattggat ataagacatc aagagttttc ctcttcgatg
     6781 tttggctata gtaaaaaaga agtccgtgct tttttagatc aagtagcttc agatgtagaa
     6841 acataccaaa agcaactgca aaaagaactt gcgcgtcaag aacagataca aaatgaagtt
     6901 aaacaagaag ctgttcaggc aggaagtgca gttgaagaat taaaaagacg ggaagaattg
     6961 attagtagaa cacttgtttt tgcagaaaaa acgaaagcag atattattgc caatgcgcgt
     7021 aaagaagcag aaaatattat taaagaggca gacctgaaag caaaaagagc tatccaggaa
     7081 gctaagcagt acttaagtgt tttggaacat caatatctac agctgaaaga acaaaaacgc
     7141 cagttcctga ttcagtttaa ggtagagctg caaagttttc aggatagaat tagtaaagac
     7201 cctctcttaa gtaaagatag tgaattaatg ctggatagcg aatttcatca gatcaaagat
     7261 gacttcgtac ctaaaccgga aaataacgaa ccgcaaaata caaagagtca ataagtgaac
     7321 aattaccaag aaactgcaat ctggttgaaa aataagttct ccgaaaaacc tctaatcgca
     7381 gtaattctgg gaacgggact aagtgattta cctgaagctt ttcctgttct atgttctttg
     7441 ccctataatc aaattcccgg tttcgtaaaa acaacctctc cttctcatcc aggtaattta
     7501 cttctatgtg aggtgaaggg aattcccatt atttttttac agggtcgttt tcattactac
     7561 gagggctatt caatgcagga agttgttttt ccagtaaggg ttttggcttc tttgggaata
     7621 aaagttttaa tagttactaa tgcctctggt agtttaagaa aagaacttaa gccaggagcg
     7681 atagtgcaat tgcaggatca tatcaacttt atgggaacta atcctttaat aggaataaat
     7741 gatgataact tgggtgagcg ctttccttca ttaaataatc cttatgatcc agaattatgt
     7801 tcacttgccg atgaggttgc caaggaactt gaaattagca catttaaagg tacatatatt
     7861 gctgttacgg gaccctcctt agaaacaaaa gctgagtgta atgcttttgc tatcttagga
     7921 gctgatttag tggggatgtc aactgttcca gaagtaataa cagcaagaca ttcaggcata
     7981 aaagttcttg cctattcaat agttactaat tacagtaatc tttttcataa tttggcacat
     8041 agccaggaag agattcaaaa aacggcattg aactctggcc gacaattaca gaaaataatt
     8101 gcagagttta tttttcgtgt aacaaagact ttttcattga cataagcagt aggtttataa
     8161 ttaatgaaaa attaggtttt ttaaatgcaa agcccaagga gataaaaatg gcagcaaaaa
     8221 aattatcgcc agacaaacta cggcatttcg aagaactgat tagaggtgaa ttaagggaga
     8281 gtttggatta tattaataat atcaataaag aacagagctt aggtgctcgt gaaagcagtg
     8341 gtgatttatc cagttatgca tatcaccaag ctgatcaagg ttcagacact aaccttatgg
     8401 aacagacagt tatgatgtta gagagcgaaa gagaaaaaat ccgtcagtta aatgaagccc
     8461 tgcgaagaat agcagatgaa acctatggag tctgtgaaat ttgcggtgaa tttattcaag
     8521 aaacgcgttt ggaaattatt ccttatgcta cttgctgtgt tgcctgtcaa gaaaaaagcg
     8581 aagataaaaa aaggcggcaa aagcgttgaa acctcccctt acaaagctaa aaaccgctcc
     8641 tgcttatttt ttaatgatgc taattgttgt tatggatcag ctaactaaga ctctggttcg
     8701 tgtaaatatg aaattatatg aacacatccc tttactggaa ggtctttttg gcgatacttt
     8761 cttacttatc aaagtagaaa atccaggagc tgcattttca atcggattag gaaatgatct
     8821 ggtaaacagg atatttttta taagtataac agttatcgtg gtaggtctta tcatttatct
     8881 attacgccgc gctcaacatc agattcaagt tattgccttt ggtttgattt taggaggggc
     8941 gataggtaat cttatagacc gtgttttgtt tggtcctgtt acagatttct tttcaatgga
     9001 ttttcctgat ttcattatgg aacgttttcc tgtgtttaac atcgctgata gtgcaatatt
     9061 tatcggtgta tgtttattaa ttattgatat gctgttcatc aaagataatt ccagtgattc
     9121 tgttgaagaa gatgaacaac taatcatcaa tgaatcagat aaggagctgt aaatgaaaaa
     9181 ctatctttta actttttttg cgcttgtttt tataacaggt atatcagctc aagcagttct
     9241 aacctgttat gatattcaat atactactga tcctacaggc aattcacctt actataatca
     9301 aacagttact gtgcaaggaa tagttactca catcattcct aattccgctt tttatattgc
     9361 tgatccggaa ggaggtgaat ggagcggact ttatgtttat catagaaata ccagcaatgt
     9421 tgtaagtgtc ggagatttag taaagcttac aggaacggtt gatgagtatt ttaacttaac
     9481 cgaatttacc aatgtatctg cttacgaaat aatatctcaa ggaaatgcct taccgccttt
     9541 tttggaatta tctacagcaa atatgccttc cggaacgaat catccggaaa atgaaaagta
     9601 tgaaggagtg ttagtaaggt ttcaggatgt aacgattaaa tcttctcctg atggttacgg
     9661 acaattcaat atagccgaca gcagcaatat ttgggcaatg gtagataatg gactatatgc
     9721 tattccagca aattcaattg ttattggtga aagttggtat attattcagg gcattgttga
     9781 ttttcatagc agtgcgggtt ttaaattaaa tccgcgtaat gccaatgata tgattaagca
     9841 ggatactatc gaaaattcca ttattaaaat tgtgagaact gatccaacta ccgatcccct
     9901 tattaataca gatgtatcta tgaatttgat aagcacgaaa ataaaagccg aatggggcgt
     9961 gcaaagttat tctgttagtt tcaagctaaa tcctgcttgc attcattttt ctggatttga
    10021 tacggaagga actttaactc catccattcc agattattat attagtagtg caggagacac
    10081 aattagctat actgttactt atcaggatgg ccttatttat aatcaaaacg atgctctttt
    10141 aattaaaatt ctggttaaac cgttggttta tggagaatct gttattgaac ttgtttcctt
    10201 taaatataac gataccaatg ttctcaacct gaatgatggc tccctggtaa ccaaactgca
    10261 aaagaagact gcttatttag atattagtaa aattggcgat aagaagaata ttttcaatcc
    10321 tgaaatgggt gaacagatta taattagtta tggaactaaa accggttatc tttccaaggc
    10381 aattgtaaga atttatgatg cgcaaggacg cttggtttat actcctgttc atactaatat
    10441 aacttcttcc acaggcatcg agaattttat ttggaatgga cgcgatgcca atttaaagtt
    10501 actgcctcca ggtttatatt actgtcatct ggaagttatc gatcggagta gcggagataa
    10561 agacaatact gttcagccca ttgttattaa gagtgcgctg aagtgaggtt agaaatgaaa
    10621 aagttattat tcgcctgtct attattaatt cctgcaacaa tgttacttgc tgtgtacgat
    10681 gattatcaac cttcagctat tgccagagga ttgggtggtg cctatactgc tgtttccaga
    10741 gatgcaaatg ccatttttta taatcctgca ggcttagtaa atactacttt gagtgccaag
    10801 cttggttttt ctcagctata caatcaggat ttcagtgaac ttaaaaccgt ggctttgggc
    10861 tataagcttc cccaaaaatt gggcacagta gctttgggga tcagggctat ggatgttgat
    10921 tatgaagatg taaatctgat gagtgaacaa gtctatagtt tggcgcacag tttttatttg
    10981 ttgcaggata tccattcgca aatatctttt ggttatacag gtaattttta tctcctaagt
    11041 atggatggtg aagatgatga tactgcccta ggtctggatt taggagctat ggcagtttta
    11101 catactagaa cgcgcttagc ttttgccgtt accaatatca ataaagccaa aatgggagat
    11161 gaaaatcaga ttgatctgcc cagtaaattg gctttaggca tatcttacat tccttacgat
    11221 aaagtaacta cttccataga agtaaaaaag gattttgcca aagaaacgga atttatgggt
    11281 ggtgtggaag ctcatctttt tgaacctttg gctatacgct tcggggttca tcaaaatcct
    11341 gctacttaca atgctggact tagtatctat attcgagatt tagaattgga ttattcttat
    11401 acttaccatt ctgttttacc tggaacacat tatttttcac tgggatataa ttttaaatga
    11461 ggataaaaaa atgggtatga aatttaaaac aatgattccg acctttattc caatagtaat
    11521 ggaaatgatg agagtattta aacgaaattc aaaacacgat caggatataa agaagaaaga
    11581 tagaacagca gaaaaactgg gttctttgga acatttgatg gtgcgtctgg aaaagcaggt
    11641 gcagcataat agagaattat acacaaagag tttaaacaaa atacgaatct ggttatgcct
    11701 caattctatc ttgctgatcg ctatcttaat aaaactgttt ttgggataag ggattaaagg
    11761 aatataatta atagatgtac agcaatctca caaaagagtg cttcagccat ggaaggcttt
    11821 cttaacgata tttaggagtc tttactgatc tgcgaatgga atattgagta ttaatggaac
    11881 gagatgcatt taaaatcttc tgttatttat gcccactacc cactaatgta tagataatta
    11941 gctataagaa gccattggat tctcaccaaa aataggagaa gaaatagagg gtatatatag
    12001 aataagaaga aatgattgac agaatgcggg gtaatatatt ttgtgaactt attgtgcgcc
    12061 cttagctcaa ttggatagag catctgacta cggatcagaa ggttaggggt tcgactccct
    12121 tagggcgtgc cacttttttc ttgccagctt ttgctggctt ttttttatag gtaaattatg
    12181 gcgaaacaaa ggaaaaatca gaagcggtgg aataatatta tcctgatttt caggatgatg
    12241 ttgcgttatt ggcattattt ggttgccggt tttatctgta tgctattttt tactcttttc
    12301 aatggagtaa gcgttacttt agctattcct ctctttgatt atgtgtttaa accaggcaga
    12361 aacaatcttc tatatacaga ttttagcgga tttctaactg ccttgggaga attaataaag
    12421 caacatttcg gttcgtctgg aggttttttt gctgccatcc agaattacaa ttccttgtgg
    12481 gaaggctgca aaaagcttat gttgcagact gattctcttt ccctgttata tggtttatgt
    12541 gtagcaattt ttgttattat cctattaaag aatggttttt atttactgca taagatattt
    12601 tttaattctt tgagaggtaa aacgatccgc gatatccgta attatatgtt tactcgctat
    12661 ttaaatcagt ccctcacttt ttttggtcgc catcgggttg gagatgcaat tgttagaatg
    12721 gtaggggatg tagatatcgt aagtgaacag ctgatcaatt cctttgtaaa tgtaactagg
    12781 gaaattttta cagttcttgt gtttgcgcgg atagcctatt tattgaatcc aactctgctt
    12841 ttatacagca tcatagttct accggttttt tctttttcga taggtctgtt aggcaagaag
    12901 ataaagaaat attcaaaacg tatacaggaa caaatctcca atctcttttc caatgtagaa
    12961 gaagtactaa acagtatgaa agttgttcaa gcctttcggc gggaagacta tgagaaaaaa
    13021 atctttgaat ccatcaacca aaaacattat cgtatgtggt ataaggcaca atattatgga
    13081 gcattgaaca cacctattgc agagctgaat actgctataa ccggtatctt agttattatc
    13141 attggtggca atatgattct ggctccagga agcaattttt ctttgggcga ttttactgct
    13201 ttcctctttg ctttattttc tatgctacat ccgctaaaag tgattaccca agtctataca
    13261 gatatccgta aggcgcttgt ttctctggat agaataggtt tggtattaaa tcaagaatct
    13321 cgcattcaag ataaacctga tgctattgtg aaagatagtt ttgatcgcga aattagcttt
    13381 gataatgtta cttttggcta taagaatact aagcctgtac tgaaaaattt caatttaacc
    13441 atctccaaag gcagtaaagt agcttttgtt ggctccagtg gagggggaaa gacaacaatt
    13501 gccaatttaa tgaatcgcct ctatgatgtg aattctggtg ctataacaat agatggtata
    13561 gatataagag atatcaaact ggatagccta agaaagctat tcggagttgt tactcaggaa
    13621 tcaattttgt tttcgcgctc aattaaagaa aatatcgctt atggttctca agatgaagtt
    13681 actgacgaac aaatcatcaa agccgcacaa attgcttatg cagatgaatt tatcgatgag
    13741 ctacctaata aatatgaaga agtgcttagc agtaaaggtt ccgatctatc cggaggccaa
    13801 aaacaacggc tttgcatagc gcgggctatt gtaggtgacc ctcctatttt aatttttgat
    13861 gaagcaactt ctgctctgga taccgattct gaacagaaag tacaattggc cattgataaa
    13921 gtaacgctaa acagaactgt aatcttgatt gcccatcggc tttctactat tttgaaagcg
    13981 gatcaaatag tggttttgga aaagggaaag attgtaggaa taggcactca tcaggaatta
    14041 ttagaaagct gttctcgata tcaacatcta tataatttac aaaacgggaa atagcaatat
    14101 gtatagatct taggtatatg aggtaacaat gaaaatcagc ggtttctctt ttgtgcgtaa
    14161 tggaattaaa ctctactatc cagtaataga atcaatccag agtattctgc ccatctgcga
    14221 tgaatttata attgctgtcg ggaaaggaga tgcagatgat gatacaaagg aacgcatttt
    14281 agccttgaac aatcctaaaa tcaagatcat cgataccatt tgggatgaga aactatgcaa
    14341 acgggggatg attaattcct ggcaaacgga tattgccaaa aaagaatgca ctggtgattg
    14401 gcttttttat ttgcaggcgg atgaagttgt gcacgaaaaa tacttacctg tgatccaaaa
    14461 acgctgtgaa gaactgttaa acgacaaaga agtggaagga ttactttttg cttataaaca
    14521 tttttgggga gattattatc actatcataa tggccatggc tggtatcctt atgaaatacg
    14581 cataattaga aataatccta atattcattc ataccaaagt gctcaatcct ttcgttattt
    14641 tgaatattat gataatccgc gtcaggaaac cggaaccagg aaattaaagg ttgccaaagt
    14701 ggatgctgaa atttatcatt acggctgggt gcgtcctccc aatttgatgc aaaataaatg
    14761 caaggcatta aattctgtcc attggggaaa ggaaaaagca gaagaatatt acaacaaagc
    14821 tcctaagtat tttgattatg gacccctttc tcaattggct ttttttgtag gaactcatcc
    14881 tatagttatg caaaatatga taactaattt taattggcaa gataaattac aattaaccgg
    14941 taaacccaat ccttatcgcg aactgcataa acatgaagaa tttaaatatc gctttttaac
    15001 ttttatcgaa aaacacttca atggtggaaa acaaatcggc actttcaaga attatgtttt
    15061 gctgaaaaga tgaatttaag tgtaattatc cccgtataca attatctgga tttattggaa
    15121 aaatgtctat tctcggttta tagacaagcg gtgcaacctg atgaaattat cctctctgat
    15181 gatggctcag aagaagatat aattggcttt ttccaggtgc aaaaagaaaa aacttcaatt
    15241 ccaatgatct tggttagaca agaacatcag caatttcggg ctgccagagt tagaaacaat
    15301 ggtgtttctg tttgcaatag tgaaattctc gcttttctgg atcaggatat tattataccc
    15361 aagaactatt tactatctat taaaaatgaa cttaccatag gtagattctt aagcggttat
    15421 cctgttcggc ttagtaaaga tcaaacagaa aaattaactt ttgaggtgat tgaacaaaat
    15481 aactggaatg ctattttaac tgaggcgcag aagaagaaaa ttatcaaaca atatagaaag
    15541 gactatttat cctatgtact ctgcaagtat ttaccttttg tagaacatgg cgctaaaatg
    15601 cgaggtggtg tttctgctat atttagaaat gattttgaag cggttaatgg atatgatgaa
    15661 aattatatcg gttggggcaa cgaggatgat gatttaggaa aacgactaca tgcattagga
    15721 agaattggct ttaactttgt actttctcaa ctccctttac atcaatatca tcaaccctat
    15781 cataacaagg gcgaaagaa
//
LOCUS       DAORHB010000007        15303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_2949_length_15303_cov_5.160808, whole genome shotgun sequence.
ACCESSION   DAORHB010000007 DAORHB010000000
VERSION     DAORHB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15303
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2949_length_15303_cov_5.160808"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PKM80_00470"
     CDS             complement(<1..822)
                     /locus_tag="PKM80_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV92409.1"
                     /translation="MLLASFLEPRNILFENRVLTKEQLYQILVDRLCSQYKLPICGSK
                     LMELILQRDEEVSPAYSTGIAIPHIRMENFNDTLIAMAFLQNPLDYNGIKVSWVVLII
                     TDKTSSKLYLNIVAALLKLSNDKEAVADLHNTGDGHSVIQYLKKKEVEVKKDVTIADI
                     MVQKPIAVHPENTLRELINLMCMYRVAGMPVVDATGKYIGEVNILNLLKVGIPDFVMM
                     LDDLSFLASYEPLENLLEKQDLLLVNDIMATDEKTLSPETSIIEAVFLMFTNKKRY"
     gene            1190..2911
                     /gene="recJ"
                     /locus_tag="PKM80_00475"
     CDS             1190..2911
                     /gene="recJ"
                     /locus_tag="PKM80_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNV92410.1"
                     /translation="MNKNWLIPAPPGKELLSDIEKLGMELKLPRLVAELLYRKGIKTS
                     SEAQEFFNPSMENLYDPFLFPDMEKAVQRILKAIDNKEKITIYGDYDVDGTTATALLY
                     LGLKRIGANIDFYIPHRMIDGYGLSLTSLDTLRENGSSLIISVDCGVNAIEEINAINS
                     MGMEIIITDHHNPKDELPPAFAIINPKLVGMKYPYEHLAGVGVAYKLLIAIYMKLGID
                     TAENKLKYMDLVAVGTIADIVPLNSENRIFASIGLQHLIEKKNLGLNALIQISGLNQK
                     NLDTSDIVFGIAPRINAAGRMGSASVSVELLISTDEAKSMELAEIIEHQNSLRQQEDQ
                     KTFQEACEIIEKKYKDMQQTSCMVISSDDWHPGVIGIVASKLVEKYYRPVIMISFKDG
                     FGSGSGRSVADFDLFEALKQTEHNLHSFGGHKYAVGLTVYQEYLDRFENELTRYVADH
                     LRLEQIQPPLQIDAEIELYDINTMLLDALDLFAPYGPDNTRPVFMTKNVTIASYPYNV
                     GRNHLKLKVVKDGINFDLIGYNMGDYLPLLRKAGKLNIAYTLEYNRFGNNVTIQGKLR
                     DLQIMKD"
     gene            2916..3752
                     /locus_tag="PKM80_00480"
     CDS             2916..3752
                     /locus_tag="PKM80_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92411.1"
                     /translation="MKQLLFLALILAYAIMLFTLFACSGINPQPPMNIEQFSLINTIP
                     LGMQTDKILYHSQSNTLYAMSVKNQEIYIFQDGELKNVLGGLGSSKVNFQYLADMALG
                     TDNSLYVLDSSTKSVKKFISDGKPSGSIELKGSVQPKCLCIYQEQEMFIFDSASGEII
                     NYSGIDGTEQNRFGRFQLQQINSLSCNHDYLIAYDKDNNLSHIFSLLGQFVKDEPGQV
                     VYDMFNNGICYNEGMLKCLNSPAYLPLTEDKVLIFIHNNILALHQGEEVRILKIVYSQ
                     VE"
     gene            3753..5243
                     /gene="lnt"
                     /locus_tag="PKM80_00485"
     CDS             3753..5243
                     /gene="lnt"
                     /locus_tag="PKM80_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNV92412.1"
                     /translation="MPPLLWVLFSALLLSLSRLPLHLGWLAFTGWIPLLYVLESRKVK
                     LWQAAMIFSLVYIGVVMYWIAGVTFLGLIGICIFYFLFYWLCFYAINRISRVSGYLFY
                     PGFILVMLSFEYLQNFGETRFPWFNNAYSLSDYTVLIQAADLGGVILISLLILVINVL
                     LYEVLIKKNRRGLLYLVGIFILWIGYGIYCLHSLPMQKQEPKIAIMQPSIPQEEKWDE
                     EAYQQILHRYDTLCAKAAEDSTKLIIFPEAAIPVYLMYDNYAKTYFRELMQKYNLEIF
                     TGFPHFTLAPDNHINDELYYNAAALFKPDGSISELYYKNILVPVGERMLWLNLFPFLW
                     KLQFGQANWEFGNGPAYYQTDSLEFSPSICYELAFAEFHQKMAIRKDKDTGSYKKTDF
                     LVNITNDAWFGTSYGPWLHSVMAKFRAVENRIQIYRSANTGISLIVDPFGRILTKAGL
                     FEITNLYAPLIICPKIPVYRYIYWYPWIFVILVAGLFIYSFLKKEA"
     gene            5257..5652
                     /locus_tag="PKM80_00490"
     CDS             5257..5652
                     /locus_tag="PKM80_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNV92413.1"
                     /translation="MKKYYYTIGEVSNLLNVKPHVIRYWESEFPWIKPKKNSGRIRKY
                     SEKQVLLLRKIYDLLYNQRYTIEGARQKIKQERTTIQEIAKDALDESSTSINEVSGMK
                     PEIKEVLLDLKNDLTQIQQTCKKYTQDKK"
     gene            5649..6506
                     /locus_tag="PKM80_00495"
     CDS             5649..6506
                     /locus_tag="PKM80_00495"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNV92414.1"
                     /translation="MNFQDIILTLQDYWAKKGCCLIQPYDLEMGAGTFHPSTFFGTLG
                     KKPCAIAYPQPCRRPKDGRYGENPNRFQHYYQFQVIIKPIPDDIQNLYLKSLAAIGIE
                     IEKHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVGGIDVFPVSVELTYG
                     LERLAMYIQNVDDYRMLAWNETTKYAELFYEREKEYSAYNFELANTKLLFQLFQDYET
                     ECNALLSKQLVYPAYDMLLHCSHTFNLLEARGVISVTERASYISRIRNMARACANNYL
                     NKINNNQEE"
     gene            6509..7168
                     /locus_tag="PKM80_00500"
     CDS             6509..7168
                     /locus_tag="PKM80_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92415.1"
                     /translation="MNKDFSNVMSTLTGIQDSLLQVAVYTDFQPQCVIILEEDPPDLL
                     ESLDSLKNYTIKRKLRFPLLINRNFAESSLDSYPLEFLDIVSSNYENLFVKKDLLKTL
                     KFDPVNLRLQMEREIKSKWLLTRLSYLEMKTDSKKMANIIALTLTNILPVLKGICYLK
                     DGSAPPQYDAAVERATNITDIEFDTIFSWIANKKVSLENVKQYLAILESLMQYLEMID
                     K"
     gene            7165..7941
                     /locus_tag="PKM80_00505"
     CDS             7165..7941
                     /locus_tag="PKM80_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNV92416.1"
                     /translation="MIYSDTYLGNSNFPILAFAIHNGHYLPEELLQGCGISEDTRLRE
                     EDPYTSIFAETFPNRIVVQTSRFAVDLNRSPEKAVYQKPEDAWGLQVRKHPVPEKLNE
                     MLIKAYEAFYRTAHYQIERLLHNNPKLIILDLHSYNHRRGGPNAAPDPQNENPDIILG
                     RNNLPESKYQTIEKLRLLIDNKPCVDSKLDCRCDVKFSGGYFSRWINSTFGDKVICVA
                     VEFKKIFMDEWTGELNLSACLQLKEIFHNAVLEWMEQVNG"
     gene            complement(8156..9655)
                     /locus_tag="PKM80_00510"
     CDS             complement(8156..9655)
                     /locus_tag="PKM80_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92417.1"
                     /translation="MKVSVCFTFLLLVCCLFLFAEVEEIPELDTENLEDNWLNLLYSE
                     IEDGKYNFGLNNRLKYSENCLTNFRLQQKELCLNLNLYFKESSELDANFQLSRLPSKN
                     NRSEIYFGSYRPAFGLGTVLKKSGEKTLFTVNRPAHPVYYSPFGAATIVRINSVSLFF
                     MGSGRKRNASLKENKINSLYKTIHSESPVVQENILAGGLEYQMNNSQFGILAYEQNYD
                     RNFVDSLMAKKMQVYAFSTKMNRENITIATELAYLEKDLALQTTSQFSYHNIAQEISY
                     AYRQGNQLPVYAAKPYLLSSKGESQEINWNLNYQPHKIVYLDLRYALIRKNNCLKSPV
                     WNSRSIINLSLKPSSTAINLQLTRLDKEIISENDSSYITTLPVHYRFRLKVRQELNEN
                     MDFTLLCRYHYEDKMELHKNSFYWENAFRYSQKRVAISTGIKTWQTLNMLIIPDEYTE
                     NPSGTIATASDENIFFIVLSYKGNLFQCKTEMQQSWLNGNRSLYFSISI"
     gene            complement(9652..10431)
                     /locus_tag="PKM80_00515"
     CDS             complement(9652..10431)
                     /locus_tag="PKM80_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92418.1"
                     /translation="MRFKVGREYIVVITTMLILLCYSSYLISVTTENSASASALSGVT
                     ILSDSPADYILSPIIGSSGICLSWQQPYSIDEINIYGLHSAFKVKPIIVATGIAYLAH
                     PDYRYQDEYLALSAEWSCFHFGLTQHLVYEKIEEQSWFTWDNDLALALQKNKIKTEIR
                     CNHLRTEDIAVTLSAAVIANESSSFSSTYSWRKNDRNNYCLATSFAIAKPLLLQCSWQ
                     NEPARFGVGMKVMLGKMSVMYAVRTHTELGLTHIVDLGSAW"
     gene            complement(10467..11570)
                     /gene="ugpC"
                     /locus_tag="PKM80_00520"
     CDS             complement(10467..11570)
                     /gene="ugpC"
                     /locus_tag="PKM80_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNV92419.1"
                     /translation="MAKVTIKNLNKYFENGFHAVKDVSFTAEDKEFIVLVGPSGCGKT
                     TILRMIAGLEDISSGEIYIGDTLVNDVPPKDRDIAMVFQNYALYPHMTVFDNMAFALK
                     MRKMPKEEIKIKVSNSAKLLGIESMLKRKPGQLSGGQRQRVALGRAIVRNPKVFLFDE
                     PLSNLDAKLRVAMRAEIVKLHKTIGNTMIYVTHDQVEAMTMADRIAVMKDGVIQQIDS
                     PLKIYNDPANVFVAGFIGSPAINMTTGKIARTNGALHFVSDDYLLKLTPRQEEILKNY
                     EDKAIIMGIRPEDIYDSRFDALADFPQKIITKVDFVEPLGNEYHVVLTTEHNEFIARF
                     DPKELPKMGQDLSITFDMTKAHFFDPTSEANLY"
     gene            complement(11644..13374)
                     /locus_tag="PKM80_00525"
     CDS             complement(11644..13374)
                     /locus_tag="PKM80_00525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV92420.1"
                     /translation="MKPSSVLFLLKMMNPINSHSNLYDISLFTSIEQEMRSTLKKLIG
                     DFIIADDSTRSEIEEQVIEIVVSACSENCENPLYCEYVGLIIQYLLDYFLQIRTTHES
                     EFYHFLNFLSHFPITQDSPYYSYIAKSAKEKQEKEQEYIELFNQLQLLYILTENGDYA
                     NAAEFLRILEPKVDINNLGLWILIQLSKAGVLRYQNQHRELLRTHLDLIIEIYNRDGS
                     DCAINLIIRWLISIPWHKQGIIKKYLLLRMYDYLEEQKSLNSAMVLYELFTLEDRFVS
                     SAEKIEYQRVLIKYPASVLNVQQLHSLYFFAGYYNCGVLSNFKDSIQNYQYSNYFLHK
                     SWDSLLSLSHFMRDCLDPEHYYSAMPFLESRIRELSNQASLQNNAYVESLQANFYKIE
                     ELYEKVGELSLTDSLTGLRNRRYLEGNIFQMIVLAARHNVPVCFSMIDIDFFKLINDN
                     YGHLVGDYVLKEIAAILSAEFRKSDVIVRYGGDEFLIILFDSDPNLSYSMMEELRNKI
                     EVHTFEYQNLKINITISIGIACDFHKSNKPTNLSKCISFADQACYAAKTNGRNRVEIY
                     KSEKQPENNC"
     gene            complement(13322..13642)
                     /locus_tag="PKM80_00530"
     CDS             complement(13322..13642)
                     /locus_tag="PKM80_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNV92421.1"
                     /translation="MKTKDKKRSPQFRFFFWGAIVLLVIWILFLANNSFLNTWKLKHR
                     VEQLEKETAALKAQNDSLAMENNRLKTDPEAAEKAARENFGLTKPDETVFRFVPAKDD
                     EPDK"
     gene            complement(13642..14766)
                     /locus_tag="PKM80_00535"
     CDS             complement(13642..14766)
                     /locus_tag="PKM80_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV92422.1"
                     /translation="MHRPAVQIRKIDNYHLPEVEEAVANFFSSIKSPKMHKAKRVLIK
                     PNALGAYAPERAITTHPIVLEAIIRYFLKRDKEIWIGDSPGGSVAFDKVWQTCGFASL
                     ADKYPVKVVNFSTTNFRTLSYQNLQIKVSEALFQCGIVINVGKYKTHQLTSFTGALKN
                     LYGFVPGLIKSEYHRANPDTTSFANLLVALYASIRNRITYSIIDGITGMDGNGPSAGK
                     PRDFGLLFGSTSIPALDYTASRIMGFRLKDVPYLSPALHLEGILPSHIDIPISFRNYQ
                     LTDADIKTVRLTQKTLKYVPGIARKAFRKVYYFYPEVSDRCRRCGICVNSCPVKAISW
                     QKNANPYIHREQCIKCLCCHELCPYQAIDIKKSFIAKLGI"
     gene            complement(14766..>15303)
                     /locus_tag="PKM80_00540"
     CDS             complement(14766..>15303)
                     /locus_tag="PKM80_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92423.1"
                     /translation="YEFSNMNMGLTMMPSSKFDWDLKYASYEAVLPLWIAGGVQFATA
                     KYGIAAEGEWEQTSDICDSFEDRYTFKAGLEKYSGDLAYRLGYHFSSNVYSGMIPLAV
                     NYANPDTSNAWDSVPELIYIKDNAQHSITAGISYYSKYGSLNLALMQVVAGDIKKTQV
                     NISLGFYLKKIIHKRNPL"
BASE COUNT         4834 a         2772 c         2722 g         4975 t
ORIGIN      
        1 atatcgtttt ttattagtga acattaagaa caccgcttcg attatagatg tttctgggga
       61 aagagttttc tcgtctgtag ccattatatc gttaactaat agcaggtctt gtttttctaa
      121 aaggttttcc aagggttcgt aagaagcaag aaagcttaaa tcatccagca tcataacaaa
      181 atcaggaatc ccaactttaa gtagatttag gatatttact tctcctatat attttcctgt
      241 tgcatccact acaggcatac cagccactct gtacatacac attaaattga tcagttcgcg
      301 caaagtgttt tctgggtgaa cagctattgg cttttgaacc ataatatcgg caatagtaac
      361 atcttttttc acttccactt ctttcttttt tagatattgg ataacgctat gtccatcacc
      421 tgtgttatga agatcagcta cagcttcttt atcattagat aactttagta aagcagccac
      481 tatattcaga tagagcttgg aacttgtttt atcagttata ataagcacca cccaagagac
      541 tttaattcca ttataatcca aggggttctg cagaaaagcc atagctatca aagtatcgtt
      601 gaaattttcc atcctaatat gagggatagc tattcctgta gaataagctg gagaaacttc
      661 ttcatcccgc tgtaatatta gctccataag tttagacccg caaataggta atttatattg
      721 actgcaaagg cggtcaacta aaatctggta cagctgttct ttagttaata ccctattttc
      781 aaacaggata ttacggggct ctagaaagct tgctaacaac atttagcacc tcataatata
      841 tataatttgt gtccttttta ataccatatt gattttagtc aatagttatt atgtagaaac
      901 ctgataattg gtgtaattag aattatactt tacatttgta atgcaggttt cgcctttgct
      961 acttgatttg ttaacctatt aatctgtgaa atggaaactg aacaagagtg ttttcttttt
     1021 gaagtgcgag ggaaaaataa ttctttacac gatatagctt tcaattatta tagtatttag
     1081 cggatacgat acgcattatt ttcttaagaa aatgttgtaa tatgctgtaa atctgttagt
     1141 ttcgtaccag aataagatac aaataaataa aaaagataaa ggcaaaaaaa tgaacaagaa
     1201 ctggttaata cctgcacctc caggtaaaga actactttcc gatattgaaa agttgggaat
     1261 ggaattaaag ctaccgcgtt tggtagctga attattgtat cgtaaaggga ttaagacctc
     1321 ttcagaggca caagaattct ttaatccttc tatggaaaat ttatatgatc cttttctctt
     1381 tccggatatg gaaaaagccg tgcaacggat tttgaaagcc atagataaca aagagaagat
     1441 aacgatttat ggcgattatg atgttgatgg cacaactgct actgccttat tatatttagg
     1501 tttgaaacgc atcggtgcca atattgattt ttatatcccc catcgaatga tagatggtta
     1561 tggtttatcc ttaaccagtt tagatacttt aagggaaaat ggttcttcac tcattattag
     1621 tgtagattgt ggagtgaatg ctattgaaga gatcaacgcc ataaattcta tggggatgga
     1681 aataataatt acagatcatc acaaccccaa agatgagctt cctcctgctt ttgctattat
     1741 taatcccaaa ctagttggaa tgaaatatcc ttatgaacat cttgctggag ttggagttgc
     1801 ttacaaattg ctgatagcca tatatatgaa actgggaatt gatactgcag aaaacaagct
     1861 taagtatatg gatcttgtag cagttggaac tatagccgat attgttcctc tcaattccga
     1921 aaatcgaatt tttgcttcaa ttgggcttca gcacttaatt gagaaaaaga acctgggctt
     1981 aaatgctttg attcaaattt ccggcttaaa tcaaaaaaat ctggatacca gcgatatagt
     2041 gtttggtatt gcacccagaa tcaatgcagc cggcagaatg ggaagtgcct ctgtttctgt
     2101 agaattgttg atttctaccg atgaggcaaa aagtatggaa ctggctgaaa ttattgaaca
     2161 tcaaaactct ttacgccagc aggaagatca aaagaccttt caggaggcct gtgagattat
     2221 tgaaaaaaaa tacaaagata tgcagcagac atcttgtatg gttatttctt ccgatgattg
     2281 gcatcctggg gtaattggaa ttgtcgcttc caaactagtg gaaaaatatt atcgaccagt
     2341 aataatgatt tcattcaaag atggttttgg tagtggttct ggacgtagtg tagccgattt
     2401 tgatcttttt gaggcactga aacaaacaga gcataatcta cacagttttg gtgggcataa
     2461 atatgccgta ggtttaactg tatatcaaga ataccttgat cgttttgaaa atgaattaac
     2521 cagatatgtg gctgatcatt tacgcttaga acaaattcag cccccactac agattgatgc
     2581 ggaaattgag ctatacgata taaacactat gctgcttgat gccttagatc tttttgcacc
     2641 ttatggtcca gataatactc gtccagtttt tatgacgaag aatgttacca ttgccagtta
     2701 tccttataat gtagggcgta atcatctgaa actaaaagta gttaaggatg gtattaattt
     2761 tgatttgatt ggttataata tgggtgatta tctgcctctt ctaagaaaag ctggtaagct
     2821 gaatattgcc tatactcttg agtataatcg ctttggtaat aatgtaacca ttcagggtaa
     2881 gttaagagat ttacaaatta tgaaggatta gccttatgaa acagcttcta tttttagccc
     2941 ttattttggc ttatgctatt atgctattta ccctttttgc ctgttctgga attaaccccc
     3001 agcctccaat gaatattgaa cagttcagtt tgatcaatac gatccccctg ggtatgcaaa
     3061 cggataaaat tctgtatcat tcacaaagca atacgctcta tgcaatgagt gtaaaaaatc
     3121 aggaaatata tatctttcag gatggagaac tgaaaaatgt tttaggtggt ttaggcagtt
     3181 ctaaagtaaa ctttcaatat ctggctgata tggctttggg aactgataat agtttatatg
     3241 tgttagattc ctctaccaag agtgttaaga aatttatttc ggacggtaaa ccttcaggca
     3301 gtattgagtt aaaaggaagc gttcaaccaa aatgcctatg catatatcaa gaacaagaaa
     3361 tgttcatttt cgattctgca agtggggaaa taattaacta ttcaggaatt gatggcaccg
     3421 aacaaaatcg tttcggcaga tttcaattgc agcagatcaa ttcactctcc tgcaatcatg
     3481 attatttaat cgcctacgat aaagataaca atcttagcca tatcttttcc ttgttaggcc
     3541 aatttgtaaa agatgaaccc gggcaggttg tttatgatat gttcaataac ggcatttgtt
     3601 ataatgaagg aatgttgaag tgtttgaaca gtcccgccta tttaccctta accgaagata
     3661 aagtactaat atttattcat aataatatct tagcgttaca ccaaggtgaa gaagtccgca
     3721 ttttaaaaat agtctattcg caggtggaat aaatgcctcc tttactttgg gttttatttt
     3781 cggctttatt gctttcgcta tcgcgtttac ctttacattt aggttggctg gcatttacag
     3841 gttggatacc cttactttat gtattagaga gcagaaaagt gaagttatgg caagcagcga
     3901 tgattttttc cctcgtttat attggcgttg ttatgtattg gatagcggga gtaacttttt
     3961 tggggttaat tggtatctgt atcttttatt ttctatttta ctggctatgc ttctatgcaa
     4021 ttaatagaat tagtagagta tctggatatc ttttttaccc ggggtttatt ttggtaatgc
     4081 ttagttttga atatctccaa aattttggtg aaaccagatt tccctggttt aataatgctt
     4141 attcgctctc cgattatact gttttaattc aggcagcaga tttgggagga gtaattctga
     4201 tttctcttct gattttggtg attaatgttc tgctttatga agttttaatt aaaaaaaatc
     4261 ggagaggact actttatctt gtcggcatct ttattctctg gattggttat ggaatttatt
     4321 gtttacattc attacctatg caaaagcagg aacccaaaat agctattatg caaccttcta
     4381 ttcctcaaga agaaaaatgg gatgaagaag cttatcagca aattttgcat cgttatgata
     4441 ctctttgtgc taaagcagcc gaagattcta ccaaactaat cattttccca gaagcagcaa
     4501 tacctgtcta tttaatgtat gataattatg ccaaaaccta ttttagagag ctgatgcaga
     4561 aatacaatct ggagatcttt actggattcc cgcattttac tttagctccc gataatcaca
     4621 ttaacgatga attatattac aatgccgcag cccttttcaa acccgatgga tccatttctg
     4681 aattgtacta taagaatatc ttggtgcctg taggagagcg aatgttatgg ctgaatttat
     4741 ttccgtttct ctggaaactg caatttggcc aagccaactg ggaatttgga aatgggcctg
     4801 cttactatca aacggacagt ttagaatttt ctccgtccat ttgttacgag cttgcttttg
     4861 cagagttcca tcaaaaaatg gctattagga aagataagga caccggcagc tataaaaaaa
     4921 ctgatttctt agttaatatt actaatgatg cctggtttgg cacttcctac ggaccctggc
     4981 tgcattctgt tatggcaaaa tttcgtgctg tggaaaacag aatccaaatt tatcgcagtg
     5041 ctaatacagg tatttcttta attgtagacc cctttggtcg cattttaact aaagcaggtc
     5101 tctttgaaat aactaacctg tatgcaccgc ttattatttg ccccaaaata ccagtttacc
     5161 gttatatata ttggtatccg tggatttttg taattctggt ggcaggactt ttcatctaca
     5221 gcttccttaa aaaagaagct taaaaagtga ggtcttatga aaaaatatta ctatactatc
     5281 ggggaagtga gtaatctatt aaatgttaag ccacatgtta tccgttattg ggaaagtgaa
     5341 tttccctgga taaaacctaa aaagaattca ggacgcattc gcaaatatag tgaaaaacag
     5401 gtactattat tgcgtaaaat ttatgattta ttgtataatc agcgctatac aattgaaggc
     5461 gcaaggcaaa aaattaaaca ggaacgaact actattcagg aaattgccaa agatgctctt
     5521 gacgaatctt ccacttcgat aaacgaagta tctggtatga aacctgaaat taaagaagta
     5581 ctactggatt tgaaaaatga tttaacccag atccagcaga cctgtaaaaa atatacacag
     5641 gataaaaaat gaactttcaa gatatcattc ttacactgca agattattgg gcaaaaaagg
     5701 gttgctgcct aattcagccc tacgatttag aaatgggagc agggaccttt catccttcca
     5761 ctttttttgg aactttaggg aaaaaaccct gtgctattgc ttatccgcaa ccatgtcgca
     5821 gacctaaaga cggtcgttat ggagaaaatc ctaatcgctt tcaacactat tatcagtttc
     5881 aagtgattat caaacccatt cctgatgata ttcaaaatct atatttaaaa agtttagctg
     5941 ctattggtat cgagatagaa aaacacgata tcagatttgt tgaagacgat tgggagtcac
     6001 caactttagg cgcttggggt ttgggttggg aagtttggtt ggatggaatg gaaatttctc
     6061 aatttactta ttttcaacag gttggaggca tagatgtctt tcctgttagt gttgaactta
     6121 cttatggttt agaacggcta gctatgtaca ttcaaaatgt agatgattat agaatgctgg
     6181 cttggaatga aaccacaaaa tatgcagaac ttttttatga gcgggaaaaa gagtatagtg
     6241 catacaactt cgaattggca aataccaaat tgttgtttca gctttttcag gattatgaaa
     6301 cagagtgtaa tgctttgcta agtaaacaac tggtttaccc tgcctatgat atgttacttc
     6361 actgttccca tacttttaat ttattagaag caagaggagt gataagtgtt acagaacgtg
     6421 cttcctatat ctcccgcatc agaaatatgg ctcgcgcctg tgcaaacaat tatttgaaca
     6481 aaattaacaa taaccaagag gaatagctat gaataaagat ttctctaatg taatgagcac
     6541 tttaactggg attcaggatt ctcttttaca ggtggctgtt tatacggatt ttcaacctca
     6601 atgtgtaatc attctggaag aagaccctcc agaccttttg gaatcacttg attccttaaa
     6661 gaattatacc ataaagagaa aattgcgttt ccctttattg atcaaccgta attttgcgga
     6721 aagctctttg gattcatacc ctttggaatt tctggatatc gtttccagca attacgagaa
     6781 tcttttcgtt aaaaaagatc tcctaaaaac acttaaattt gatccagtaa acctgcgttt
     6841 acaaatggaa cgcgaaatta aaagcaaatg gttgcttact cgtttgtcct atctggaaat
     6901 gaaaaccgac tccaaaaaga tggctaatat tattgcctta actctcacaa acattctgcc
     6961 agtattaaaa gggatttgtt atttgaaaga tggctctgcc cctccccaat atgatgccgc
     7021 tgtagaaaga gcaacaaata ttaccgatat cgagtttgat acaatatttt cttggatagc
     7081 taacaagaag gtaagcttag aaaatgttaa acagtatctt gccattttag aaagccttat
     7141 gcagtacttg gaaatgatag ataaatgatt tacagcgata cctatctggg aaattctaat
     7201 tttcctatcc ttgcttttgc catccataat ggccattatt tgccagaaga acttctccag
     7261 ggttgtggaa tttccgaaga taccagatta cgagaagaag acccatatac aagtattttc
     7321 gcagaaacct ttccaaatag aattgttgtt caaaccagtc gctttgccgt agatttgaac
     7381 cgttctccag aaaaagctgt ttaccaaaaa ccagaagatg cctggggctt acaagtgaga
     7441 aaacatcctg tgccagaaaa acttaatgaa atgcttatta aagcttatga agcattttat
     7501 agaactgccc actatcaaat tgaacgctta ctacataaca accctaaact gattatttta
     7561 gatttacata gctataatca tcgcagaggt ggaccaaatg cagctccaga ccctcaaaac
     7621 gaaaatcccg atattatttt gggaagaaat aatctgccag agagcaaata tcaaacaata
     7681 gaaaaattgc gtctactgat cgataacaaa ccctgtgtag attcaaaact ggattgccgc
     7741 tgtgatgtta aattctcagg tggatatttt tctcgttgga taaacagtac ttttggtgat
     7801 aaagtaatct gcgttgctgt agaatttaaa aaaatcttta tggatgaatg gacaggagaa
     7861 ttaaacttat ccgcttgctt gcagttgaaa gagatatttc ataatgctgt tttagaatgg
     7921 atggaacagg ttaatggatg aataagtgat aggtttttaa tgaggttata aatagttata
     7981 tcttttttgt gtgatggact tatgcattta gtgctaaagt aatcactacc aacatcgtaa
     8041 cccactaaat ctctataagc taaagaaaat ataactgcag gaaacacatt tttctccttt
     8101 agccacactc catataatta atggaaaggc cgttgctgat ttatatcttc ttgtattata
     8161 tactaataga aaaatagaga gagcggttgc catttagcca agattgttgc atttccgttt
     8221 tacactgaaa caaattccct ttatagctta gcacaataaa aaatatgttc tcatcacttg
     8281 ctgtggctat agttcccgag ggattttctg tatattcatc aggtataata agcatattta
     8341 aggtctgcca agtttttatt cctgtagaaa tagcaactcg cttttggctg tagcgaaagg
     8401 cgttttccca atagaagcta tttttatgaa gttccatttt atcttcatag tggtaacggc
     8461 agagcaaggt aaaatccata ttttcgttca gttcctgcct aacctttaga cgaaaacgat
     8521 aatggacagg gagggtagta atataactac tatcgttttc tgatataatt tctttatcca
     8581 atctggtcaa ttgcagattt atcgctgttg aagaaggttt caaagaaagg tttattatac
     8641 tgcgggaatt ccaaactgga cttttcaggc aattattttt cctgattagg gcatagcgca
     8701 agtccaaata cactatttta tgtggctgat aattcagatt ccagttaatt tcttggcttt
     8761 ctcccttact acttaaaaga tatggtttgg cagcataaac tggtaattga tttccctggc
     8821 gataggcata gctaatttct tgagcaatat tatgatagct gaattgtgaa gttgtctgca
     8881 aggcgaggtc tttttccaga taagcaagct cagtagctat cgtaatattt tccctattca
     8941 ttttggtaga aaaggcataa acttgcattt tttttgccat aagtgaatct acaaagtttc
     9001 tatcgtagtt ttgttcatat gccaaaatgc caaattggct attattcatt tgatattcca
     9061 atcccccagc aaggatattc tcttgcacta cagggctttc agaatggatt gttttataca
     9121 gagagtttat tttattctct tttaaggaag cattgcgttt tcgtcccgaa cccataaaaa
     9181 ataaggatac actatttatc cgcacaatcg ttgccgctcc aaaaggtgaa tagtatactg
     9241 gatgagcagg tctattaaca gtaaaaagag ttttttctcc gctttttttt aatactgttc
     9301 ccaatccgaa agctggacga taggaaccga aatatatttc ggaacggtta ttttttgagg
     9361 gtaaacggga cagctgaaaa ttagcatcta attctgaact ttctttaaaa taaaggttta
     9421 gattcaagca tagttctttt tgctgtaacc gaaaattagt aaggcaattc tctgaatatt
     9481 ttaatctgtt attcagtcca aagttatatt tcccgtcctc tatttctgaa tataatagat
     9541 tcaaccagtt gtcttcaaga ttttctgtat ctaattctgg aatttcttca acttcagcaa
     9601 ataggaaaag acaacaaact aacagtagga aagtgaaaca aactgaaacc ttcaccaagc
     9661 agaacccaaa tccacaatat gcgttaagcc caattctgta tgagttctaa ctgcatacat
     9721 cacactcatt ttacctagca taaccttcat cccaactcca aaacgagctg gttcattttg
     9781 ccaagaacat tgaagtaata agggttttgc tatggcaaaa gaagttgcca ggcagtagtt
     9841 atttctgtcg tttttgcgcc aagaatatgt agatgaaaaa ctggaagatt catttgctat
     9901 cacagctgcc gataaagtta cagctatgtc ttcggttcta agatggttac agcgtatttc
     9961 cgttttaatt ttatttttct gcaaagcaag tgctaaatcg ttatcccagg taaaccaact
    10021 ttgctcttct atcttttcat agaccagatg ttgagttagc ccaaaatgaa aacaagacca
    10081 ctcagcactt aaagccaaat attcatcttg ataccgataa tccggatgtg ccaaatatgc
    10141 aataccggtt gccacaataa tgggctttac cttgaaggca ctatgcaaac cgtatatatt
    10201 gatttcgtcg atactatagg gttgttgcca gctaaggcaa attccactgg aacctataat
    10261 tggagataat atataatcag caggtgaatc agaaagaata gttaccccag ataaggcact
    10321 tgcagaagca gaattttccg tagttaccga tatcaaatag ctactgtaac aaagcaggat
    10381 gagcattgta gtaataacca caatatactc tctccctact ttgaacctca taggataaat
    10441 cctttggtta cataaaggtt gctagcttaa tataggtttg cttcgcttgt tggatcaaag
    10501 aaatgagctt tagtcatatc aaaagtaata cttaagtctt gtcccatttt cggtaattct
    10561 tttggatcaa agcgtgcgat aaattcatta tgttcagttg ttaaaaccac atgatattcg
    10621 tttcccaatg gctcaacaaa atctaccttg gtaataattt tttgggggaa atctgctaag
    10681 gcatcaaacc ttgaatcgta tatatcttca ggtcggatac ccataataat agctttatct
    10741 tcataatttt ttaagatctc ttcctgacgg ggagtaagtt ttaacaaata atcatcgctt
    10801 acaaaatgca gagcaccatt agtgcgagca attttccctg tagtcatatt aatagcagga
    10861 ctaccaataa aaccagcaac aaatacatta gctggatcgt tatatatttt caggggagaa
    10921 tcaatttgct gaataactcc atctttcata acagcaatac gatctgccat cgtcatagct
    10981 tctacttgat catgtgtaac ataaatcata gtattaccga ttgttttatg caatttcaca
    11041 atttctgctc tcatagctac tctcaatttt gcatctagat tagaaagggg ctcatcaaaa
    11101 aggaaaactt tcggattacg aacaatggct cttcctaagg ctactcgctg tctttgtcca
    11161 cctgaaagtt gacctggttt tcttttcagc atactctcta tgcccaataa ttttgctgag
    11221 ttactcactt ttatcttgat ttcttctttg ggcatttttc tcatttttag ggcaaaagcc
    11281 atattgtcaa acaccgtcat atgaggataa agagcatagt tttggaagac cattgctatg
    11341 tctctatcct tgggaggaac atcattaact aaggtatctc caatatagat ttcaccagaa
    11401 gagatatctt ccaaaccggc aatcattctt agaatagttg ttttcccaca acctgaaggg
    11461 ccaactaaaa ctataaattc cttatcttcg gcagtaaaag aaacatcttt tacggcatga
    11521 aagccatttt caaaatactt attcagattc tttattgtta ctttcgccat ttctaaactc
    11581 ctatataatt acttattgaa agcaattggt atatatgtca agtgggaaat catagatatg
    11641 catctaacaa ttgttctcag gttgtttttc tgatttataa atttcaacgc ggttccggcc
    11701 attcgtttta gccgcataac aggcctgatc tgcaaaggag atacatttag aaagattagt
    11761 gggtttattg cttttatgaa aatcacaagc aataccaata ctgatcgtaa tattaatctt
    11821 cagattttga tattcaaaag tatgtacttc aatcttgttc cgtagttctt ccatcataga
    11881 ataacttaaa ttaggatcag aatcgaacaa aataattaag aactcatctc ctccataacg
    11941 cacaattaca tcacttttac gaaactcggc actaaggata gcagcaattt cttttaagac
    12001 ataatcacca accagatgtc catagttatc attaatcaat ttaaagaaat cgatatcgat
    12061 catactaaag caaacaggga cattatgtct ggcagctaaa acgatcattt gaaagatatt
    12121 accttccaaa tatcgtctat tgcgtaaacc cgttaagcta tctgttaatg aaagctctcc
    12181 aactttttca tagagttcct caattttata gaaatttgcc tgcaagcttt ccacataagc
    12241 attattctgt aaagatgcct gattacttag ttctctgatg cggctttcta aaaaaggcat
    12301 agctgaataa taatgttcag gatcaagaca atctctcata aaatgcgata aactgagcaa
    12361 actatcccaa cttttatgca gaaagtaatt ggaatattgg taattttgaa tcgaatcctt
    12421 aaaattggaa agtaccccgc aattatagta tcctgcaaaa aaataaagcg aatgtagctg
    12481 ttgcacattt aatacagaag ccggatattt aattaatacc cgttgatatt ctatcttttc
    12541 tgcagaagaa acaaaacgat cttctaaagt aaaaagttca tagagcacca tcgcactatt
    12601 caaagatttc tgttcttcga ggtaatcgta cattcgtaat aataagtact ttttaattat
    12661 gccctgctta tgccagggaa tagaaattag ccagcggata attaaattta tggcacaatc
    12721 gctgccatct ctattgtaaa tttcaattat taagtccaaa tgtgttctaa gcaattctct
    12781 atgttggttt tgataacgca aaacacctgc tttggaaagc tgaataagaa tccataatcc
    12841 caaattattt atatctactt tcggttccag aattcttaaa aattctgcgg catttgcata
    12901 atctccattt tcggttaata tatataaaag ctgaagttga ttgaagagtt ctatatattc
    12961 ttgttctttt tcctgttttt ctttcgcact cttggcaata tatgaataat agggtgaatc
    13021 ctgagttatg ggaaagtggc tcaggaaatt gagaaaatga taaaattcac tttcgtgggt
    13081 ggttcttatc tgcagaaaat aatccagcaa atattgaata atcaaaccta catattcaca
    13141 atatagcgga ttctcacagt tctcagagca ggcagaaact actatctcta taacttgttc
    13201 ttcaatttct gaacgggtac tatcatcagc tataataaaa tcaccaatta gcttttttag
    13261 cgtggatcgc atttcctgtt caatactggt gaaaagagat atatcgtaaa ggttggaatg
    13321 actatttatc gggttcatca tctttagcag gaacaaaacg gaagacggtt tcatctggtt
    13381 tagttagccc aaaattttcc ctggcagctt tttcagctgc ttcaggatct gttttcaagc
    13441 ggttattttc catcgctaaa ctatcatttt gagctttcag ggcagcagtt tctttctcca
    13501 actgttcaac cctatgcttc agtttccaag tgtttaaaaa actgttgtta gctaaaaata
    13561 aaatccagat aaccagcaga actattgctc cccaaaagaa aaaacggaac tgtggacttc
    13621 gcttcttatc ttttgttttc attatatccc caatttagct atgaatgatt tcttgatatc
    13681 tattgcttga taaggacaga gctcgtgaca gcataagcat ttaatacatt gctctctatg
    13741 aatataggga tttgcattct tctgccagct gatagccttc actggacagc tgttcacgca
    13801 gataccacat cttctacagc ggtcactaac ttcaggataa aagtaataaa ccttccgaaa
    13861 tgctttacga gcaatgcctg gtacatattt cagggttttc tgagttaaac gcacagtttt
    13921 gatatctgca tccgttagtt gatagttacg aaaagaaata ggaatatcaa tatgcgacgg
    13981 taatatgcct tctaaatgca aagcaggact taaataggga acatctttca gtctaaaacc
    14041 cataatccga gaagcagtat aatccaaagc aggtattgat gttgaaccaa aaagaagccc
    14101 gaaatctctg ggtttaccag ctgaaggacc atttccatcc ataccagtta taccgtcgat
    14161 tatgctataa gtaattctgt tacggatact tgcatacaga gctaccaaca aattggcaaa
    14221 acttgttgta tctggatttg ctctatgata ttcactttta ataagcccag gcacaaatcc
    14281 gtaaagattc ttcagagctc ctgtaaaaga agttaattga tgagttttat acttgcctac
    14341 attaatcact ataccacatt ggaataatgc ttcgctaact tttatttgca gattctgata
    14401 gctgagtgtc cgaaaattgg tagtggaaaa atttaccact tttacaggat atttatctgc
    14461 aagagaagca aatccacagg tctgccacac tttatcaaaa gctacagaac ctcccgggct
    14521 atctccaatc cagatttcct tatccctttt cagaaaatag cggataattg cttctaaaac
    14581 aataggatgc gtagtgattg cacgttctgg agcataagcc cctaaggcat ttggttttat
    14641 taaaaccctt tttgccttgt gcatttttgg ggatttgata ctggagaaaa aattagcaac
    14701 cgcttcttct acctcgggca gatggtaatt atcaattttg cggatttgca ctgcaggtct
    14761 atgcatcaaa gtgggttcct tttatgaatg atttttttca gatagaaacc aagggatata
    14821 ttcacctgag ttttttttat gtctccagca acaacctgca tcaacgcaag attcagagag
    14881 ccatattttg aataatagct tatacctgcc gtgatagaat gttgggcatt atccttgatg
    14941 tatattagtt ccggaacact atcccaagca ttggaagtat caggattggc atagtttact
    15001 gccaagggta tcatcccact atagacattg gaactaaagt gataacccaa acggtatgcc
    15061 agatccccac tatatttttc caatccagct ttaaaggtat aacggtcttc aaaactatca
    15121 caaatatcgc tggtttgttc ccattcgccc tctgccgcaa ttccatactt cgccgtggca
    15181 aattgaaccc ctcccgctat ccataaaggc aaaacagctt cataactggc atattttaaa
    15241 tcccagtcaa atttggagga aggcatcata gttaaaccca tattcatatt gctaaattca
    15301 tat
//
LOCUS       DAORHB010000008        15038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3026_length_15038_cov_4.927718, whole genome shotgun sequence.
ACCESSION   DAORHB010000008 DAORHB010000000
VERSION     DAORHB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15038
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3026_length_15038_cov_4.927718"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="PKM80_00545"
     CDS             complement(<1..1720)
                     /locus_tag="PKM80_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNV92424.1"
                     /translation="MKRLSIFLGIITFISLCYSIQVSGNQSGIWSPENNPYEVIGAIT
                     VPSTDSLIIQAGVEVHITGSYQITVQGILNAYGTEADSIRFLNMQTYPTALWPGLRFE
                     NLQLTSHLNYVYIEYATYGVRTLNSPIAISHCHLNLCEKGIELYGIGSAIPLPNVVEY
                     SLIENCTQNAILVTSNSNAYIRYNEIRYNGTGPQFRAAIQMGNQSGVNQCNPNILNNH
                     IHHNYKQGISAWDIASSGSINPQILNNLIEYNYTGIYLLQASGYVADNQINFNFIPGD
                     MNSGAGVMVSGVTSIPYFERNHIEGNYTGFYITNNGKPVLGDLAIDHIWAQGENTIIN
                     NIDASGNNNSVFCDAYANASFIIMAENNFWGFSSAAEIAETINDHNDNAALPTVDFEP
                     FIIPVENTTITGNYTYNGTAQIASARLELITVSESNIQVTVPLSELNFSLETDIAEDF
                     YVQVVLTETATGKDFYGCAGGYSNPTVFSPNPGNSINVGTIEVTDSPLPRYEYVAEPF
                     QENNLTLYPVATGRGLYSYDKLDILYAEGDYLYLKRNFWQTPTGEVVTELPVGTIYKK
                     YLNITAG"
     gene            complement(1978..2148)
                     /locus_tag="PKM80_00550"
     CDS             complement(1978..2148)
                     /locus_tag="PKM80_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV92425.1"
                     /translation="MAVKIDKETCLGCGACVDGCPVSALKMVDGKAECDASLCIDCGA
                     CIATCPVQAISE"
     gene            2332..3633
                     /locus_tag="PKM80_00555"
     CDS             2332..3633
                     /locus_tag="PKM80_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HNV92426.1"
                     /translation="MFSKDIQSTGRPYKEIEKRAIIFKEEEGKLYHFTKRYIKVIRLG
                     NPYPAAYQKKLKDNYWECFLPTQPFPFLFYPPKPENLKSNNYAIRLNALHKESIYKSM
                     EAYIGAEKIELLNGFTSRFWFILCLLFRGGNYALELMQTNPAIGYLLSAYAVFHPLKS
                     KKYWQSSTKLITKKRKDILGYFGFPACETMVKTFAKIKPESCSTNIFFILRTSLQNNP
                     NLLRTLSYFPVHNDYSLFLVCKRLDIMQNYNLIAEIANMNQEEVLNINSQINDIYRML
                     EVLRENGLQDRKVILKNITDLNTYHDELVIQILKLKKIDFVPLPEGIAKDIKCHTLWI
                     EHIHNSIELYREGMEMHHCIYSYLSDIRAKTRFVARMLYPERLTILYENTKNDGLKLK
                     DARGKYNTTPDQNSFCLIKLWLAGVEMDIIDSYEPISQTSH"
     gene            3850..4983
                     /locus_tag="PKM80_00560"
     CDS             3850..4983
                     /locus_tag="PKM80_00560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV92427.1"
                     /translation="MKICIVTNTHPTNDVRLYYKLAMSLAKENEVYLITTNGVINNKL
                     NPYQMVVDASSRWAAIWQLGKKVRDLKPDILICVEPLTLLIAWVLKHKLQLKVIFDVH
                     EFFAIAFSERFPRFLRFPAYLFYQVSLKQLLKIADAVFTVNREMEKQLLSGNKRITSI
                     VLPNYPVKNVWDYECNKPGSLEQICEMNFDFIYIGGLTEDRGVFKILKMVSILKQDFP
                     FLKVLIVGKFFKKEVEQRFNQSINDYNLNALIYYQSWIPAEKIGLLLKRSKFGLWIFN
                     PKNKRLQLSTPLKVLEYLAAGLPVLTIKTPIMKALIEKNGLGICSAYQSKALADACAK
                     LLNLKQSEYQAMSDKCIKLTENKFNWEALEPELFKIINGLNSK"
     gene            4980..6269
                     /locus_tag="PKM80_00565"
     CDS             4980..6269
                     /locus_tag="PKM80_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV92428.1"
                     /translation="MKILYISYFYPPLAGPASLRNLKTVKYLSQLGNTIDVLTVGNIV
                     YNFYDNGLLAKCEQNRIIHIPSFDPMSILEKLSGKNKTMSNNIYHQTPERIKLFIRHL
                     YPLDDKVLWLPNLLKTAKKTLAGDSYNLIYVSCGPFSSAVAARYLSKHFKLPYVLDMR
                     DYWTLLNDYNLQGTYLNRLHSQHHEKRILKDAALIVTATKGIAEDISNHFNKELQSKT
                     FVLYNGHDEEDFSTLPKMETNHDQYTLSYFGTITAKRSLKWLLRALKNLKQENKLPVG
                     FSFNLYGNYHRETYQEIQQSGIEEIVKVTPQITHKEALAVMQASDALLLLINSDSPKG
                     TLTSKVFEYLRIGKPILALIPKQGEAAELLKESGQNFICPMESSVAIKKELEKLFAQR
                     DDEHHFHYLAEKYERKKQVEELNQKLQKINKTQFNNT"
     gene            complement(6312..7409)
                     /locus_tag="PKM80_00570"
     CDS             complement(6312..7409)
                     /locus_tag="PKM80_00570"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNV92429.1"
                     /translation="MKKRLLVATGGGDCPGLNAVIRAVVKRASHEHNWEVLGSVDAFN
                     GILQDPMHIIELTPERVAGIHVQGGTIIGTTNKGGPFTWPVKNQDGTWSTVDRSQDFM
                     DRLRYQNIEALINIGGDGSQRISHKLFEIGCPIIGVPKTIDNDLSSTDFTFGFQTAVD
                     VATDAVDKLVTTAASHHRILILEVMGRYAGWIALHSAIAGGAEVCLIPEIPYSIDKVM
                     DAINQRIDKGHDFANIVIAEGARPIDGEMTYIESEEPGAMKMKLGGAGLRLSQELKDA
                     GCTIDIRETILGHLQRGGTPNAFDRILASQFGVKAFELVLEQKWGSMVAYHHPNIVAV
                     PLAEAIRKYNYIDVSSDLVATARGLGISLGD"
     gene            complement(7450..7884)
                     /locus_tag="PKM80_00575"
     CDS             complement(7450..7884)
                     /locus_tag="PKM80_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV92430.1"
                     /translation="MLTIKRIKQLNNNLYQKVSELWVETGISNPERKDSLEIVQQNLE
                     NTGTMIIAEEDENLIGVVWVTHDFRRLYIHHMAVKPNKQNQGIGTKLMEECVTIAKEF
                     GYQPKLEVSIDNPAARHLYEKFGFKELTGYSVLIKRNILTDL"
     gene            complement(7887..8810)
                     /locus_tag="PKM80_00580"
     CDS             complement(7887..8810)
                     /locus_tag="PKM80_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNV92431.1"
                     /translation="MKKNKLILLLILLLLIGLYFLLRTKQPQEKLLPVFDLDTLAINR
                     IEIYDAQDTLKMVKQGNCWRLIYPVNWEADSLKIHSLFKDILTAKYSKTPMGTGKEAI
                     LKFHQRDEETLHLIVSDGKKTVHTLFSNLNNPYDYFRYAGSEEIYQVKTKVTNVYNTD
                     LANWRSPHIVSHQENDLVKIEVTHPKNKYTLTRKGLDWYYQDAHQNFQIHPYNRAMIK
                     ILNILEHLDTYIFVDNAQAEYAAKFDDPYCTVKLFLTNNRTQDLKFIEHYQGHYLMMV
                     DNDPTVLFVVVWDTVFRFTRPPDVFRMQELG"
     gene            complement(8797..10371)
                     /locus_tag="PKM80_00585"
     CDS             complement(8797..10371)
                     /locus_tag="PKM80_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNV92432.1"
                     /translation="MKKAKKISSILSNLLIKLGIVLMILLIGSYAKIRWDFSHNKAYS
                     LSKISKYAVSKLEDNMVVKLYASKELPPQMNTIDRYVKDLLEEYRQAGKGKFHYDIIT
                     GLTQEELKNKTQLYGIPTMYFQIFENDKTTTKEIIYGLVFEYQGNYESLYVLPEMQNK
                     LEYEMTLKIQKISRYTLPDVTAFTDSLYTIMPKQKYENELNSNYNVHFTNLKEPPQQT
                     EVMIFHSGFDSLTTVQLYNLDQYIMKGGKLVVLADKMASDGERVLELDSNLFDILENY
                     GIKFSNAIAMDIFCDSRQMGVDTNISFPVYPVLRGSQHPITQNISNIVIYMGNGIIFN
                     KKPGLKFQTILATSTSSAFLEGPDYQLDASMFLHPDPEVFVNPPIPLGAIVEGRMESY
                     FAHKPDAKQPGFISEVKDGKIVVFADRELYIDPDNPIYENRYHIILNAVDWLMDRPDM
                     IAIRSRHLQKSILDIPYYMHKHNLVWGEPVKTERRIKLIIKIVAIVLPSLLLVGIGWI
                     SSIMQKRNLLLKNEEE"
     gene            complement(10372..11094)
                     /locus_tag="PKM80_00590"
     CDS             complement(10372..11094)
                     /locus_tag="PKM80_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV92433.1"
                     /translation="MTTVFTIAKKEYQVAMRLVSTYIVFILFLVVIGVFFSNMVFKIG
                     QAELRFLFEMIHIIFLFYIPAITMGSIAKERQSGTLELLSTLPIKLSSIIWGKILAAL
                     YQIITIIFLSLTFFVIIVIFGEGIDYGAIICGYIGLIMVGFAYASIGVFASSFPSNQI
                     LAFVLALLISAAFYVLKFVLPLLPFRVLPVVQYLSFDYHLSSFLKGVIDTRDILFFLG
                     VTVIFALLAQFNLQSRNLMQER"
     gene            complement(11091..12107)
                     /locus_tag="PKM80_00595"
     CDS             complement(11091..12107)
                     /locus_tag="PKM80_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV92434.1"
                     /translation="MIKIENLSRNFGNIKAVDDISFCIQDREILGFLGPNGAGKTTTL
                     RMIVGYLQPTSGNIEIDGESIFANPIKASSQIGYLPEHNPLYDEMTVIELLAYFASLR
                     KLKRDYFKERLDYVVKNCGLKEVLYQKIGTLSKGYRQRTGLAQAILHNPRILIMDEPT
                     SGLDPNQIIEIRELIRELGKEKMVLLSSHIMQEVQALCDRIVIINKGKIIVDDTKDNL
                     PNYLKKSRLLTLEVQGDNVDFSDFLAMYPFLEMEVFLQTETICKMGFYETSEIDLRKE
                     LASFISQKGWLILELSTSKLSLESIFHELTEGEYSSAKTEVASPEQEEQNAHITSIDE
                     EEQQ"
     gene            complement(12110..13282)
                     /locus_tag="PKM80_00600"
     CDS             complement(12110..13282)
                     /locus_tag="PKM80_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV92435.1"
                     /translation="MRKKWWIIIGIIVLIVAGLLFAKSCGKKKQPEKVQSTEEETYTV
                     NRGDISSRIEITGEVQPKAMVSLKSKVSGKIVKFYADENDYVTSGQIIADIEPDYNQA
                     NTLFNTKAMLQKAEINLKNARKDLADKTVLLKKQFISQEEYDKASDALQEAEIEYAQA
                     SSQYEMIRDLDVPGKVTHVYATSSGVVIERNINEGEMVQSSLTSYGEGTVIMKIADLN
                     QMIIKSNINEVDISKFKLSQNAEISLDALPYEKYEGRVVRIAPQAIIENNAKVFPIEI
                     SINTNGKIAKPGMTANVTILADSRENVLVIPIRAVFSNDKNQDIVYLVTGNTTSTPAT
                     KANDKEKTSATPNYVSTPIKLGTNDLLQVEVIEGLKEGDIILLNEPGTAKNNFMMM"
     gene            14286..>15038
                     /locus_tag="PKM80_00605"
     CDS             14286..>15038
                     /locus_tag="PKM80_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92436.1"
                     /translation="MNKYSAIVLMGIALWACSIQAGVIIVAADGTGQVTSIQAGINLA
                     AVGDTVLISPGVWTGVVVLDNKPITIGSYYITDGDTTHISQTIIDGEDIRTGIIIKNC
                     SGAVDTLKVIGLTLRNCRSNNYPLAYEYTSGGGIGVMRSVAEISKCVIQQCRAYYGGG
                     ISVIYSTVLLRSNEIFGNVAENAGGGISAIKPGTGIIFDQNELNSVYLNYSSTGCDIA
                     YAVTCNPTVIYLGNGSVTTNDPYFYFCPGQTPI"
BASE COUNT         4792 a         2859 c         2668 g         4719 t
ORIGIN      
        1 ccccggcggt aatattcaaa tattttttgt aaatagtgcc tacaggtagt tcagtaacta
       61 cttccccggt gggagtttgc caaaagtttc ttttcaggta tagatagtca ccttcagcat
      121 aaaggatatc caacttgtca taactgtaaa gtcctcttcc cgttgccaca ggatagaggg
      181 ttaaattatt ctcctgaaat ggttctgcca catattcata acggggcaga ggactatcgg
      241 taacttcaat tgtgcctaca ttaatggaat ttcctggatt gggtgaaaaa acagtaggat
      301 tgctataacc tccggcacag ccataaaaat ctttgcccgt tgctgtttcg gttagaacaa
      361 cctgcacata aaaatcctca gcgatatctg tttcaaggga aaaattaagc tcggaaaggg
      421 gaactgtaac ttgaatatta ctttcggaca cggtaattag ttccaagcgt gctgaagcaa
      481 tttgtgctgt gccattataa gtataattgc ctgtgatggt agtattttcc acagggatta
      541 tgaagggctc aaaatctact gttggcaaag cagcattatc attgtggtcg ttgatggtct
      601 cagcaatttc ggctgccgaa gaaaagcccc aaaaattgtt ttctgccata ataataaagc
      661 tggcattagc ataggcatcg caaaaaacag aattattatt gccagaagca tcgatattgt
      721 tgataatggt gttttctccc tgcgcccaga tatgatctat agccaggtct cctaaaactg
      781 gtttaccatt attagtaatg taaaatccgg tataattacc ctcaatatgg ttgcgctcaa
      841 aataagggat ggatgtaact cccgaaacca ttactcccgc tccggaattc atatctcccg
      901 gaataaaatt gaagttgatt tgattatccg ctacataacc tgatgcctgt aataaataga
      961 tgccggtata gttatattca atgaggttat ttaaaatctg gggattgatg gaacccgaag
     1021 aagcaatgtc ccaggcagaa attccctgct tgtagttgtg atgaatatga ttatttaaga
     1081 tattgggatt gcattgattt acgccagatt gattgcccat ttgaatcgcc gctcgaaatt
     1141 gaggtccggt tccattatag cgaatttcgt tatacctgat ataagcattg ctattagagg
     1201 taaccaaaat agcgttttgc gtgcaatttt caattaatga atactctact acatttggta
     1261 atggaatagc agaaccgatg ccataaagct ctattccctt ttcacaaaga tttagatgac
     1321 agtgagatat ggcaatgggt gaatttaaag tgcgcactcc ataagtggca tattctatat
     1381 agacataatt taagtgactg gtaagctgta aattttcaaa acgcaaacca ggccaaagag
     1441 cggtaggata tgtttgcata ttcaaaaaac gaatgctgtc tgcctctgtt ccataagcat
     1501 tcaaaattcc ttgaacagtt atttgatagg aacctgttat atgaacttcc acccctgcct
     1561 gaatgattaa agaatctgtg gaaggaacag ttatagcacc tatcacttcg tatggattat
     1621 tttccggaga ccatatacca ctttgatttc cactcacttg aatggaatag caaagtgata
     1681 taaaggttat tattccaaga aatatactta agcgtttcat cttttcacct ccttttgggt
     1741 tttgtattaa tcaaatttga tctgatggaa acgaaacata tttataagat aattgatttc
     1801 ctcattactt acaaagaaaa aattgccaaa gcagcaactt aatgaatgta tgagtttccg
     1861 aactactatt ctatccttga agaggataaa ttgacaatat aagccatcca ctgtggcaaa
     1921 aaagaaatca aattccaatt ttgccacagc gttttagcta aactattatt acttagatta
     1981 ttctgaaatt gcctgaactg gacaagtagc aatacaggca ccgcaatcta tgcaaagtga
     2041 tgcatcacat tctgctttgc catctaccat ttttaaagca gaaactggac atccatcaac
     2101 gcaggcacca catccgagac aagtttcttt atcgatcttg acagccattg tgcgtctcct
     2161 tgatattttt tctcaactat tttatttgct ggggtttcgt caaggaaaat atccttattt
     2221 ctcacttctg ttttttcttg acagtatagt tatctattta cccaatgaac ttatccgatt
     2281 tcttgaaatg ctgataaatt caaggctatg aaaaaagata ggaggcgatc tatgttttca
     2341 aaggatattc aatctactgg cagaccatat aaggaaatag aaaaaagagc aataatcttt
     2401 aaagaagaag agggaaaact ctatcatttt accaaacgct atataaaggt tattcgtttg
     2461 ggaaatccct atcctgctgc ttatcaaaaa aagctaaaag acaattattg ggagtgtttt
     2521 cttcctaccc aaccctttcc ttttctattt tatccgccaa aacccgagaa tctaaaaagc
     2581 aataattacg ctattcgttt gaatgctcta cacaaagaat ctatctataa aagtatggaa
     2641 gcttatatag gagcggaaaa aattgaactg ctgaatggtt ttacttcccg cttttggttt
     2701 attttatgtc tgctcttcag aggaggaaat tatgcgcttg aactgatgca aacaaatccg
     2761 gcaattggtt atttgctaag tgcatatgca gtttttcatc ctctaaaaag taaaaaatac
     2821 tggcaatctt ctaccaaact gattaccaaa aaacgcaaag atattctggg ctattttggt
     2881 tttcccgctt gcgaaacaat ggttaagacc tttgccaaga taaaaccaga atcttgttcc
     2941 actaatattt tctttatctt aagaaccagt ttgcagaata atcctaactt actgcgtaca
     3001 ctttcttatt tccctgtaca taatgattat agtttatttc ttgtatgcaa aagattagat
     3061 ataatgcaaa actacaatct aatcgctgaa attgccaata tgaatcaaga ggaggtcttg
     3121 aatataaact ctcaaataaa tgatatatac cggatgcttg aagttttgag agaaaacggg
     3181 ctacaggata gaaaagtgat actgaaaaac ataaccgatt tgaatactta ccacgatgaa
     3241 ctggtaatac aaatattgaa attaaaaaag atagactttg tgccgttacc ggaaggcatt
     3301 gccaaagata ttaaatgcca tacgctctgg atagagcata ttcataattc catagaatta
     3361 tacagagaag gaatggaaat gcatcattgt atatattctt atcttagcga tatccgcgct
     3421 aaaacacgct ttgtggctag aatgctctat cccgagcgtc taactatctt gtatgaaaat
     3481 acaaaaaatg atgggttaaa actaaaagat gcccgcggaa aatataatac aactcctgat
     3541 cagaattctt tttgcctcat taagttatgg ctggcaggcg tagaaatgga tataatagat
     3601 tcttacgagc caatctccca aacctcgcat tagcatttgg gaaactggga tatagcattg
     3661 ttcaataatt gagagttagc gtattttatt tatccatcct ttcagagcca aattatgggg
     3721 cgcggtatgc tcttttgttt tggtatgctt aaataagtta tcgatgcaat aaccccactt
     3781 ttcctcttga cataaatgct tatgctttaa tataatgtaa aagattaacc taaaaaggca
     3841 gtcctgaaaa tgaagatatg tattgttacc aacacgcatc ctacgaatga tgtgcgctta
     3901 tattacaagc tggcaatgag tttagccaaa gagaatgaag tttatttgat tactaccaac
     3961 ggagtaataa ataacaagct caatccctat caaatggttg ttgacgcttc ctcccgttgg
     4021 gctgccattt ggcaattggg taaaaaggtt agggatttaa aacctgatat cctaatttgt
     4081 gtggagccct taactttgct gattgcctgg gttttgaagc ataaactgca gctaaaggtt
     4141 attttcgatg ttcacgaatt ctttgcgatt gccttcagtg aaaggtttcc ccgttttttg
     4201 cgttttcctg cttatctttt ttatcaagta tctttaaagc agttgctgaa aatagccgat
     4261 gcagttttta ccgtaaacag agaaatggaa aaacaattgc taagtggaaa taaacgcata
     4321 acctctatag ttttacccaa ttatccggta aaaaatgttt gggattacga atgcaataag
     4381 ccgggctctt tggaacaaat atgtgagatg aactttgatt ttatctatat tggtggatta
     4441 acggaagaca gaggagtatt taaaatcttg aaaatggttt ccatcctaaa acaagatttt
     4501 ccttttctaa aagtgctgat tgtgggcaaa ttcttcaaaa aggaagtgga acagagattc
     4561 aatcaaagca tcaatgatta taacttgaat gcgttaattt attatcaaag ctggattccg
     4621 gcagaaaaaa tcggtctttt gctaaaacgc agtaaattcg ggctttggat atttaatcct
     4681 aaaaataagc gcttgcaact ttccaccccc ctaaaagttt tggaatatct ggctgccggg
     4741 cttccagtat taactatcaa aacccccata atgaaagcac tgattgaaaa aaacggttta
     4801 ggtatctgct cggcttatca atctaaagca ttagccgatg cctgtgctaa attgctaaat
     4861 cttaaacaaa gtgaatacca ggcaatgagc gataagtgca taaaacttac tgagaacaaa
     4921 tttaattggg aagcgctgga accagagctt tttaaaataa taaatggact taactctaaa
     4981 tgaaaatact ctatataagt tatttctatc cccctctggc gggacctgcc tctttacgaa
     5041 acctgaaaac ggtgaagtat ttaagccaac tgggtaatac aattgatgta ttaaccgtgg
     5101 gcaatattgt ttataacttc tacgataacg gattgttagc taaatgcgaa caaaatcgca
     5161 ttatccatat tccgtcattt gatccgatgt ctatcttgga aaagctaagc gggaaaaaca
     5221 aaacgatgtc aaataacata tatcatcaaa ctccagagag aattaaactc tttatccgcc
     5281 atctttatcc tttggacgat aaagtgcttt ggctgccaaa tttactaaag acagctaaaa
     5341 aaacccttgc gggagatagc tacaacctaa tctatgtttc ctgtggacct ttttcttctg
     5401 ctgtggctgc aagatattta agtaaacact ttaagttacc ttatgttttg gatatgcgcg
     5461 attattggac tttgctaaat gactataatc tgcagggaac ttatctaaat cggcttcatt
     5521 cccaacatca tgaaaaaagg attctaaaag atgcagcctt aattgttact gccaccaaag
     5581 gcatcgcaga agatattagt aaccatttta ataaggagtt acaaagcaaa accttcgttc
     5641 tctataacgg ccacgatgaa gaagactttt ctaccttacc caagatggaa acaaatcacg
     5701 atcaatatac tttaagctat tttggaacca ttacagctaa gcgttcttta aaatggttgc
     5761 taagagcatt aaaaaatctg aaacaggaaa ataaattgcc tgttggtttt agctttaact
     5821 tatatggaaa ctaccataga gaaacatatc aggaaatcca gcaaagcgga attgaagaaa
     5881 ttgtgaaggt aactccacaa attacacata aagaagcctt ggcagttatg caagcttctg
     5941 atgccttgtt gcttttaata aattctgata gccctaaagg aacccttaca agcaaggtgt
     6001 ttgaatattt aagaatagga aaacctattt tagctcttat ccctaagcaa ggagaagctg
     6061 cagaacttct aaaagaaagc ggacagaatt tcatctgccc aatggagagc tctgtggcta
     6121 taaaaaaaga actggaaaag ctatttgcac aaagagatga tgaacatcat tttcactatc
     6181 tcgcagaaaa atatgaaaga aagaaacagg tggaagaact gaatcagaaa ttacaaaaga
     6241 taaataaaac acaattcaat aatacttaaa cccttgtttc taatatgtta tctgagagag
     6301 accatccaat atcaatctcc taaagaaata cccagtccgc gggcggttgc taccaagtct
     6361 gaagaaacat caatgtaatt atattttctg atagcttcag ctaaaggtac agctactata
     6421 ttgggatgat ggtatgctac catagagccc catttttgtt ctaaaacaag ttcaaaagcc
     6481 tttactccga attgagaagc caaaattcgg tcaaaagcat taggggttcc acctcgttgt
     6541 aaatggccta aaatggtttc gcgaatatca attgtgcagc cagcatcttt taattcttgt
     6601 gaaagccgta atcctgcacc ccctaatttc attttcattg ctccaggttc ttcactttct
     6661 atataagtca tttctccatc tatgggtctg gctccttcag cgataacaat attggcaaaa
     6721 tcgtgacctt tgtctatgcg ctgattaatt gcgtccataa ctttatcgat gctataggga
     6781 atttcaggta ttaagcaaac ttccgctcct ccagcaattg cagaatgaag tgcaatccat
     6841 cctgcataac gacccataac ttcaagaatt agaatacgat gatgacttgc agcagtagta
     6901 acaagtttat ctactgcatc ggtggctaca tctaccgcgg tttgaaagcc aaaagtaaaa
     6961 tctgtagaag aaagatcgtt atcaatggtt ttgggaacac cgataatggg acagccgatt
     7021 tcgaaaagct tatgtgaaat gcgttgagaa ccatcacctc cgatattgat taaagcctcg
     7081 atattttgat accggagacg atccataaaa tcctgagagc ggtctacagt ggaccaagtt
     7141 ccatcttgat ttttaacagg ccaggtaaag ggtcctcctt tgttagtagt tccgataatt
     7201 gttcctccct gaacatgaat gccagcaact cgttcaggag tgagttcaat gatgtgcatt
     7261 ggatcttgta gaataccatt aaaggcatca acgctaccta aaacttccca attgtgttca
     7321 tgtgaagctc tttttacgac tgctctaata actgcattta agccaggaca atcgcctccg
     7381 cctgttgcca ccagtaatct ttttttcata gcaacctcct tttaggcact aaaaaaattt
     7441 gctttgggac tataagtcgg ttagaatatt tcttttaatt agaacgctgt atccagttag
     7501 ttctttaaat ccgaattttt catatagatg cctggcagct ggattatcaa tactcacttc
     7561 caatttaggt tgataaccaa attctttagc aattgtaaca cattcttcca tcagtttggt
     7621 tccgatcccc tgattttgct tatttggttt tactgccata tgatgaatat acagacggcg
     7681 aaaatcgtga gttacccaaa caacacctat cagattttca tcttcttcgg caattatcat
     7741 agttccggta ttttccaaat tttgttgcac aatttccaga gaatctttac gctcaggatt
     7801 gctgatacct gtttccaccc aaagctcaga tactttttgg tatagattat tatttagctg
     7861 ttttattcgt tttatagtaa gcatactcag cccagctcct gcattctaaa aacatcagga
     7921 ggtctggtaa aacggaaaac agtatcccag acaactacaa acaaaacagt tggatcatta
     7981 tctaccatca ttaaataatg cccttgatag tgttcaataa atttcaaatc ctgtgtgcgg
     8041 ttattggtta agaataattt tacagtgcaa tagggatcat caaatttagc agcatattcc
     8101 gcttgagcat tatctacgaa tatataagta tccaggtgtt ccagaatatt taggattttt
     8161 atcatagctc ggttataagg atgaatttga aaattctgat gtgcatcctg ataataccaa
     8221 tccaagccct ttctggttaa agtgtatttg tttttgggat gagtaacctc aattttaacc
     8281 agatcatttt cctggtggct aacgatatga ggtgaacgcc aatttgccaa atcggtatta
     8341 tatacattgg taactttagt tttcacctga taaatctctt ccgacccagc ataacgaaaa
     8401 taatcgtatg gattattcaa attgctaaaa agggtatgca cggttttttt gccgtcgctg
     8461 acaattaaat gtaatgtctc ttcatctctc tggtggaatt taagaattgc ctctttgcct
     8521 gttcccatcg gagttttaga atatttggca gttaaaatat ctttgaatag ggagtgaatt
     8581 tttaggctat ctgcttccca attaactggg tatattaatc tccagcaatt accctgttta
     8641 accattttga gagtatcttg ggcatcgtaa atctcaattc tattgatggc taaagtatcc
     8701 agatcaaaaa caggaagaag cttttcctgg ggttgctttg tccgcaaaag aaagtataac
     8761 ccaatcagca aaagcagaat tagcagcaga atcagtttat tcttcttcat tttttaagag
     8821 cagattgcgt ttttgcatta ttgaacttat ccatccaata ccaaccagca ataaagaagg
     8881 taaaacaatg gcaacaatct tgattatgag tttaattctg cgttcggttt ttacaggctc
     8941 gccccaaacg agattatgtt tgtgcatata ataaggaata tccaaaatgc ttttctgtaa
     9001 atgacgggaa cgaatagcaa tcatatctgg acggtccatt aaccaatcca cagcattcag
     9061 aataatgtga tagcggttct cgtaaatcgg attatcggga tcaatatata gctctctatc
     9121 cgcaaaaaca acaattttac catctttaac ttcagaaata aaaccgggtt gtttggcgtc
     9181 tggcttatgg gcaaaataac tttccattct gccttccact atggctccca agggaattgg
     9241 cggattcaca aaaacctctg gatcggggtg taaaaacata cttgcatcca gttgataatc
     9301 gggaccttct aaaaatgcgc tgctggtaga agtagccaga atagtttgaa actttaaacc
     9361 tggcttttta ttaaaaataa tgccatttcc catataaatt acaatattgg atatattctg
     9421 tgtgatggga tgttgagaac cccttaatac aggataaaca ggaaaagaga tattggtatc
     9481 cacacccatt tgtctagaat cgcaaaaaat gtccatagct atggcgttgc taaatttaat
     9541 accataattt tccagaatat caaagaggtt agaatccagc tccagaactc tttcgccatc
     9601 cgaagccatt ttatctgcta aaacaactag tttacctcct ttcataatat actgatctaa
     9661 gttatacagc tgaacggttg ttaaagaatc aaaaccagaa tgaaagatca ttacttctgt
     9721 ctgttgcgga ggttctttca gattagtaaa atggacatta taattagaat taagctcgtt
     9781 ctcatatttc tgtttgggca ttattgtata tagagaatcg gtaaaagctg taacatcagg
     9841 caaagtataa cgggatattt tttgaatctt tagcgtcatt tcgtattcca gtttattttg
     9901 catctcaggc aacacatata atgattcgta attgccctga tattcaaaaa ccagaccata
     9961 gataatctct ttagtagtag ttttatcgtt ctcaaaaatc tggaaataca tagtaggaat
    10021 gccatacaat tgagttttat ttttcagttc ttcttgggtg agcccggtaa taatatcata
    10081 gtgaaattta cctttgccag cttgtcgata ctcttctaaa aggtccttca catagcggtc
    10141 tattgtattc atctggggtg gtaattcttt actggcataa agcttaacta ccatattgtc
    10201 ttccagtttg ctcactgcat acttgctgat cttgctaaga gaataagctt tattgtgtga
    10261 aaaatcccaa cgtattttgg cataagaacc aatcagcaag atcatcaaaa caatccccag
    10321 tttgattaat aggttggata aaatggagct tatctttttt gcctttttca tttaacgctc
    10381 ctgcatcagg ttcctgctct gtaaattaaa ttgagccagt aatgcaaaaa taacggttac
    10441 accaagaaag aacagaatat cgcgcgtgtc gatcactccc tttagaaaac tacttaaatg
    10501 ataatcaaaa ctcaagtact gtacaactgg aagaaccctg aaaggtaaga gtggtaaaac
    10561 aaatttcaac acataaaaag cagctgaaat tagcaacgcc agaacaaaag ccaaaatctg
    10621 attactggga aaactggaag caaaaacacc tatggaagca taagcaaaac caaccataat
    10681 taatcctata tatccacaaa ttattgctcc gtagtctatt ccttcaccaa aaatcacaat
    10741 gattacaaaa aaagttaaag agaggaaaat aattgttatg atctgataca atgcagccag
    10801 aatttttccc caaattatgg aagagagttt tatcggaagc gtggataata attccaaagt
    10861 tccgctctgt ctctcttttg ctatgcttcc catcgttatt gcaggaatat aaaataggaa
    10921 aattatatgg atcatctcaa atagaaaccg tagctcagct tgtccaatct tgaaaaccat
    10981 attagaaaaa aagaccccaa taaccactaa aaaaaggata aacacaatat aggtagaaac
    11041 caaccgcata gctacttgat attctttttt ggcaatagtg aaaacagttg tcattgttgc
    11101 tcctcttcat ctatagaagt tatatgagca ttttgttctt cttgctcagg ggaagcgact
    11161 tctgtcttcg cagaactata ttcaccttcc gtaagctcat ggaaaataga ttccaagctc
    11221 aatttactgg tagaaagttc caaaattaac caacccttct gactgataaa actcgccagt
    11281 tctttgcgca aatcaatttc cgaggtttca taaaaaccca ttttgcagat agtttcagtt
    11341 tgcaaaaaca cttccatttc caaaaaagga tacattgcca aaaaatctga aaaatcgaca
    11401 ttatcaccct gaacttccaa ggttagcaat ctgctcttct tcaagtaatt tggtaaatta
    11461 tctttcgtgt catccacaat tattttgcct ttattgataa ttacaatcct gtcacataaa
    11521 gcctgaacct cctgcattat atggctggaa agcaaaacca ttttctcttt accaagctct
    11581 cgaataagct ctctaatttc aattatttga ttggggtcaa gcccggaagt aggctcatcc
    11641 ataattaata tgcgtggatt atgtaaaatt gcctgcgcta aacctgtgcg ttgacgataa
    11701 cctttggata gcgttcctat cttttgatat aagacctctt ttaatccaca gtttttaact
    11761 acataatcta agcgctcttt aaaataatct cgttttagct ttcttaatga agcaaaatat
    11821 gccaataact caattaccgt catttcatca taaagcggat tatgctcagg taaataacca
    11881 atttggctac tggctttaat cggattggca aagatgcttt ctccatctat ttctatgttt
    11941 cctgaagtgg gttgtaaata gcccacaatc atcctaaggg tagttgtttt acctgctcca
    12001 ttaggcccta aaaagcccaa aatttctcta tcttgaatgc aaaagctgat atcgtcaaca
    12061 gctttaatat tgccgaaatt acggcttaaa ttctcaattt ttatcattat tacatcatca
    12121 taaagttatt ctttgcggtt ccaggttcat tcagcagaat tatatctcct tctttaagcc
    12181 cttcaatcac ttccacctgt aataaatcgt tagtaccaag ttttatggga gttgatacat
    12241 aatttggagt agcacttgtt ttttctttat cattggcttt tgttgcggga gtagaagttg
    12301 tgtttcctgt taccagataa acgatatcct gatttttatc gttgctaaaa actgctcgaa
    12361 ttggtattac caaaacattt tcacgagaat ctgccagaat agttacattt gctgtcattc
    12421 caggttttgc aattttacca ttggtgttaa tactgatttc aatagggaaa accttggcat
    12481 tattttcgat tatagcttga ggggcaattc taacaactct accttcgtat ttctcatagg
    12541 gaagagcatc taacgatatt tccgcatttt ggctaagctt gaacttggaa atatccactt
    12601 cgttgatgtt acttttaatg atcatttggt tcaaatcggc aattttcatt attactgtgc
    12661 cttctccata gctcgttaaa ctggattgaa ccatttctcc ttcgtttata ttgcgctcaa
    12721 taacaactcc gctggaagtg gcataaacat gagtaacttt tcctggaaca tcaagatcgc
    12781 ggatcatttc atattgactg ctagcttgag catattcaat ttcggcttcc tgtaaagcat
    12841 cggatgcctt atcatattct tcctgtgaga taaattgttt ctttaaaaga acagtcttgt
    12901 cagccaaatc cttacgcgca tttttcaggt ttatttctgc tttttgcaac atcgctttcg
    12961 tgttaaacaa tgtatttgcc tgattatagt ccggttcaat atcggcaatt atctgtccgg
    13021 aggtaacata atcattttca tcagcataaa attttactat tttaccactc accttggact
    13081 tcagagaaac cattgctttg ggttgaactt caccggtaat ttctatccgg gaagatatat
    13141 cgccccgatt tacagtgtag gtctcttctt ctgtcgactg aactttttcc ggttgtttct
    13201 ttttgccgca gctcttggca aaaagcaagc ccgctacaat cagaacgatt attccgatta
    13261 tgatccacca tttcttacgc ataatacctc tatttttttg actatttcac ttataataaa
    13321 acacaagtgg aaaaagcaga atttgtgtca acttaaaaag tcattcgctt tctttgttgt
    13381 cgccctttga gttaaaatcg gtgttgtttg tagtcttcca catcatttca cccttttctc
    13441 tatctttgca taaggtcacc aattatctca tagtatatgc cgattttgac ttaaagccga
    13501 caacctgatt atctcgctct ttgagttaaa atcggtgttg tttatcgtct tccacatcat
    13561 ttcattcttt tctctatctt tgcataaggt caccaataat ctcatagaat atgccgattt
    13621 tgacttaaag ctgactcaga caacttaata gaaagcgtca atcaagtgat aatctaacct
    13681 taatcaagcg ttaactatta acgcttgata aacgcctgat gaacgataga tgaatggctt
    13741 ctggcaagga accgataaag tagcaatatc agaattttat cataacagtg cttattaaag
    13801 tttgtattat accgtcataa gagtgaaatt gtctcataat ttttaagccc ttacctctat
    13861 gcttctaatt gcaataaaca cttatatata acctataaga atgccctttt actttctttt
    13921 cctatcagct acataaaatc aatgcatctg ccaagagtgt tgttgagatc agtatttata
    13981 accttaaagg ccagttggtt aaaacactaa acacaaccga aatctcaaag ggtaacactt
    14041 cctttatatg ggatggcaaa acagacgatg gaagacaagt cgcccaaggt atatactttt
    14101 gtaggatcaa atatccaaac aatctattga caaaaaagtt gattgtatta aactaagaaa
    14161 agataagttt ggtaaagcta tcaggtgggg ctaagtcccc tcctgatagt gtctacaaaa
    14221 aaaagaagta aaatattagt aagataaatg ttcatttaag tagataggat attgggagcg
    14281 gaattatgaa taaatattca gcaatagttc tgatggggat agcattgtgg gcttgttcta
    14341 tacaagcagg ggtgatcata gtcgcagctg atggaacagg acaagtaact tccattcaag
    14401 caggaataaa tttggctgca gtgggtgata ctgtgctcat ctcaccaggg gtttggactg
    14461 gagttgtagt cctggataac aaaccgatca ctattggcag ttactatatt actgatggcg
    14521 atacaaccca catcagccag acaatcatag atggggaaga tatacgaaca ggcattatca
    14581 taaaaaactg cagcggagct gttgatacat taaaagtgat tggacttact ttaagaaatt
    14641 gtcgcagtaa taattatcca cttgcatacg aatatacatc tggtggcggc attggcgtta
    14701 tgcgaagtgt cgcagagata tcaaaatgcg taatacaaca gtgtagagca tattacggag
    14761 gtggaattag tgtaatctac tcaactgttt tgcttagaag taacgaaata tttggtaatg
    14821 tggctgaaaa tgcaggcgga gggattagtg caattaagcc agggacagga attatctttg
    14881 atcaaaatga attaaacagc gtgtatttga actacagtag cactggctgt gatattgcgt
    14941 atgccgttac atgcaatcct actgttatct atttgggaaa tggctctgtt actacaaacg
    15001 atccctactt ctatttctgc ccgggtcaga caccgatc
//
LOCUS       DAORHB010000009        14603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3197_length_14603_cov_4.991133, whole genome shotgun sequence.
ACCESSION   DAORHB010000009 DAORHB010000000
VERSION     DAORHB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14603
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3197_length_14603_cov_4.991133"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PKM80_00610"
     CDS             complement(<1..918)
                     /locus_tag="PKM80_00610"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV92437.1"
                     /translation="MIISKLKEIFVSLTIRNYRLFFMGQGISLIGTWIQRTTMGWFVY
                     RLTGSAFLLGLVSFLSMVPSVFVSPFAGAWADRVNRHHIMIYTQIAFCLQTGILAILV
                     LTGVINKQVQYPIIILSLLQGIIDAIDAPIRQNFVMDLVSSRRLLPNAIATNSAMFNG
                     ARLIGPAIGGFLIMMFSEGVCFAINAISYIPVIISLCFIRISYPPPSPQKEPTLKKIV
                     SGWKYAWENMPIRFLISNLAIYTIFGMSFSTLMPIFAKDILQGTSATQGLLMSAMGVG
                     ALAGSFFLASRKNIRGMPTRLVYLCLVYSI"
     gene            complement(921..2282)
                     /gene="hslU"
                     /locus_tag="PKM80_00615"
     CDS             complement(921..2282)
                     /gene="hslU"
                     /locus_tag="PKM80_00615"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNV92438.1"
                     /translation="MLNYVHLTPKRIVEELDKYIIGQDQAKRCVAIALRNRWRRQQIS
                     GDIKGEIIPNNIILIGPTGVGKTEIARRISRLTGAPFVKVEASKFTEVGYVGRDVESM
                     VRELMNYAVSQIREMMIEEVRPEAEKNATEMILDILLPRSKKMKQNRVSDNAEYDRYL
                     RSREKMRKKLLSGSLDDKEIEIEPELDTTPNLEILPGPGLELLDLDFTEMLTNIFQGK
                     GASRTIKTTVHTAWKRYIETEINRLISKDKLIETAKNSVEENGIIFIDEIDKIANIDK
                     QGGLDVSRSGVQRDLLPIVEGSKVPTKYGMIDTSHILFIAAGAFHSAKPSDLIPELQG
                     RFPIRVELNSLTKEDFERILIEPENSLTKQYQEMFRSEGVELTFMLSAIREIARFAAL
                     ANEKMEDIGARRLHTVMNTLLDDFLFEMPSETLKSVKITKKMVTEYLNPVIESEDLSR
                     FIL"
     gene            complement(2286..2816)
                     /gene="hslV"
                     /locus_tag="PKM80_00620"
     CDS             complement(2286..2816)
                     /gene="hslV"
                     /locus_tag="PKM80_00620"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNV92439.1"
                     /translation="MIMHGTTIIGIRRNGKTAICGDGQVTMGEAIVKGNAQKIRRIFD
                     GKVIVGFAGATADAFSLLERFEDKLKSNKGNLRKAAIDLARDWRQDRILRRLEAMLIA
                     GDKDTLLLINGVGDVLEPDDNIIAIGSGGNYALAAARALARKTKLGAGEIAVEAINIA
                     SEICIYTNSNYIVEEV"
     gene            complement(2819..5023)
                     /gene="ftsH"
                     /locus_tag="PKM80_00625"
     CDS             complement(2819..5023)
                     /gene="ftsH"
                     /locus_tag="PKM80_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNV92440.1"
                     /translation="MMKYILILFCIIALGFLNATTADTLTVPQKTLPEAGNAFMQFST
                     LMNWFILALLLISIISIIRSIIVRNRNIKNKDKPNPDFRNPQPPKNVPPKKSAFSLPM
                     IIMLILMVIIIFHIFSTSGTSITKESYTNFMARVANGQITQVQFSEKDIFYTDIANKK
                     YTTTLPFENPQLVDSLVAMGIKVSATKPSRWASIISYLLPFLLLIIFWAFFLRGMNAQ
                     NAKAFSFGKSKARLYEASKTGVTFKDVSGVDEAKEELQEIVEFLKDPKKFQRLGGRIP
                     RGVLLIGRPGTGKTLLAKAVSGEAGVPFFSISGSDFVEMFVGVGAARVRDLFEQAKKN
                     APCITFIDEIDAVGRHRGSGLGGGHDEREQTLNQLLVEMDGFEPNEAVIIIAATNRPD
                     ILDPALLRPGRFDRRVTVDLPDIKGRTEILKVHTAKVPLANDVHLEIISRGTPGFSGA
                     DLANLVNEAALIAASKNKTQIQMDDFEEAKDKLILGKEKKSRVIPEEDKRLTAYHEIG
                     HVLTSVFLDKVEAVHKVSIIPRGFTGGATHYLLSDKTNYSKSYLLQLLVTLLGGRAAE
                     EVIFTEFTTGSGNDLERSTDIAKKMVCSWGMSDKIGPITIGKEQGEVFLGKELVSRDI
                     YSDETSQMVDREIREIINNAYSQAISILNEHKELMEIMANELFDKETLGTDEIFKLIL
                     DNCRDDESEIVQNKYKKALELRFEPGEKARVSEPETLEDKTEDTDTKLEETS"
     gene            complement(5020..5565)
                     /gene="hpt"
                     /locus_tag="PKM80_00630"
     CDS             complement(5020..5565)
                     /gene="hpt"
                     /locus_tag="PKM80_00630"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNV92441.1"
                     /translation="MNKMNCDISAVLFDEHKIQTRIRELGLEISNSYKDSTPVMVGIL
                     KGGFIFLADLVRSITIPVEVDFLAISSYGNDANSSGIVKIRKDIDIDLSNRDVIIVED
                     IVDTGLSLEYIKDYIWQHKPASLKTCVLLDKPDAHKIDVCFEYIGFSIKNEFVVGYGL
                     DFAERYRNLPYIGILKEELYK"
     gene            complement(5562..6995)
                     /gene="tilS"
                     /locus_tag="PKM80_00635"
     CDS             complement(5562..6995)
                     /gene="tilS"
                     /locus_tag="PKM80_00635"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNV92442.1"
                     /translation="MEIAQALQRLEKNINENELIKIGDKLLLGCSGGADSNAMLYLFS
                     QLRVTYNLTLLAVHVNHQIRGAESDADQESVKELCSNLNIPLIIHKVKVPKTGNLENH
                     ARKLRFSVFAKVLDSYKFDKILLAHQREDQAETVLLNLFRGSGITGMAGIKAISGKIV
                     HPMLCFSRKELEDILSKVDITWRTDSSNLDNKFRRNLLRNELIPRLEREFNPMLSSHL
                     SFVAELFAQADKILKQRSESQYKRICLDIQPERTVLSCPLLIKLSKIERYYLLRNVFI
                     KVCDDDNDFFAVHYQAIDNILATKGSKCIYLPKNIVVIKQYEELIIQVGDIKKNTEPE
                     PMIIDTDRTMAVYGNYRFSFKYLKVQPKDHKLADGQEQIIIDADKVNFPFMIRFRKPG
                     DRFMPFGMTQYKSLKEFFIDEKVPKYERDLIPILEDGEKLFWIVGCRLDDRVKCDETT
                     SRYLQIVANPIASKSRRAIHKKEKRKG"
     gene            7570..8838
                     /gene="hutI"
                     /locus_tag="PKM80_00640"
     CDS             7570..8838
                     /gene="hutI"
                     /locus_tag="PKM80_00640"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNV92443.1"
                     /translation="MSEETMQADLIIINISKLVTLAHNGRPRLGKEMEETATILNKAL
                     AVKDGKILEINDSKSIEEKYRNVECIDANGQIATPGFVDCHTHPIFVHTREDEFAMRI
                     QGKTYVEISKAGGGILNTIQTTRDADEDLLFELAKRRILKMIQQGTTTLEAKSGYGLD
                     TENELKQLRVIRRLQEELLIDIVPTFMGAHEFPPEYRNNHSGYVELLCKEMIPAVAEQ
                     GIAEFCDVFTEAHTFNIEESRKILSCAAEYGLKLKMHSDEIESIGGAELASEMKCISA
                     DHLGSASETGIKALQKAGVVPVLLPATLFSLQSKKYANAKLMMELNLPVAIATDFNPG
                     SCNCDSMSLTMSLACLQMGMTPAASLCAATLNAAFALDRGKIIGSLETGKQADIVLWD
                     IPDLNFIPYHLGSSYITSVLKNGKIVYKAT"
     gene            8839..10866
                     /locus_tag="PKM80_00645"
     CDS             8839..10866
                     /locus_tag="PKM80_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNV92444.1"
                     /translation="MADTSLNSYQIIKTVNSSLRFSLYQAKQIETGNVVLIKTPDSQR
                     TNDIGLKHDLVNEANSHLKLNHPFIRTVYETREEKGTAYLIGEYIEGRSLSAYLKNNP
                     NSFTLEESLSRLQELLEAISYAHKMGCIHLNLNPYNILVDKENHLHIIGFGKNQDAYK
                     TAYEKDETYHPLLYIAPELYSAGIALPQSDLYSFAVIAYHILCGVIPWRVDNQLIPEK
                     QKQQSLCRAVIMPEILNKQVPDWLFAALLQCLKLDPHLRFSNAEELLELFTKRDNWVP
                     EQEAEIIKEVEKVETIVDIEKCEAIEKIEQIEDVQLVDDLPPTNLALNVDSQQKINTD
                     ISHLYGKLDVTIPEEIPQSDSVKTISDTTPKDTPKYSFTTSAPVETIETRKLKKTFKV
                     LIWMSAIVLLFIIIKYVAFGNRPKFTSVADSTQVEIPEGPLNKNLPIAMMLVPADTLV
                     MGNIAPEADDDEFPLLTISIPAFYIGQKEISQAEWMMVFPNNPSHSKDPALPVENVSF
                     YDIIEFCNQKSVLDGFEPCYDYYDNEVVCNFSSNGYRLPTEAEWEFAAKSGKRYDFFV
                     YSGSADPDEVGWYNSNSEAQSHPGGQKKPNQLGIYDLSGNLFEWVWNWYAPYSARNWN
                     LITGPDKGTDKVIRGGSWYHSVSEMRVTNRNYAKPYTKNAYLGFRVVRTKS"
     gene            11122..12198
                     /gene="prfB"
                     /locus_tag="PKM80_00650"
     CDS             11122..12198
                     /gene="prfB"
                     /locus_tag="PKM80_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNV92445.1"
                     /translation="MNKYLKFGGYFDPEPKKLLISELEQKMNAPDFWLDQTNAKKVSK
                     QLSQLRDELDHIRKMENIKGELETYLSLLEEDFNIDLLNEAVSELPRIQNFIEKAEIE
                     CYLNDKYDHNDALLTIHSGAGGTESQDWVEMLLRMYSHWAEKNNYSFNIIDYLPGEEA
                     GIKSVSVEIKGDFAYGMLKSEIGIHRLVRISPFNAQGKRQTSFASVFVYPEFDEDLDV
                     EIDPKDLKIDTFRSSGAGGQYVNTTDSAVRITHLPTNIVVTCQNERSQIQNREKAMSI
                     LKSRLYQYYEEQREKEKQNVESSKTEIGWGNQIRSYVFQPYQMIKDHRTNYETGNTDK
                     VMDGDLNDFIYAWLKYNAKQRING"
     gene            12191..12931
                     /gene="uppS"
                     /locus_tag="PKM80_00655"
     CDS             12191..12931
                     /gene="uppS"
                     /locus_tag="PKM80_00655"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNV92446.1"
                     /translation="MAKQDYKQLLNELDRDKLPKHIGFIMDGNGRWAKKRNKPHLYGH
                     RAGVKSLRQVVELGVELKLQYLTFYAFSTENWSRPESEVKGLLWLLKDQLKKEIPELM
                     EQNIYAQFIGTSIGLEKNYWNEVSSLVAQTHKNTGMIVNFAFNYGGRLEIIEGFKKFM
                     SMNPDKWDKLTPEDFGNYLWTAGQPDPDLIIRTSGEKRLSNFLLWQSAYAEIYITETL
                     WPDFDKTEMIKALLDYASRDRRFGGRKE"
     gene            12928..13728
                     /locus_tag="PKM80_00660"
     CDS             12928..13728
                     /locus_tag="PKM80_00660"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNV92447.1"
                     /translation="MNELFKRVLVAALLIPVVLIILYFQGLSLVITLAIIVFLGGLEY
                     IRMMHKAKIQISYVWIVLNCLFYFAIVYIKGRDFSLLWLALLFALIESITTWNAERSI
                     PRLFATFFCFVYTALFPALIARISLSYSEYNFLLALILMIWFVDSIAYFVGMKIGKHR
                     NITAISPQKSLEGFLAGILAPWLILIILYICKVRIMSFSYLALIAIAAGIIGQIGDLI
                     ESMLKRYCKVKNSSNLLPGHGGILDRCDSILFAGSFLYCALEILTKVR"
     gene            13734..>14603
                     /locus_tag="PKM80_00665"
     CDS             13734..>14603
                     /locus_tag="PKM80_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNV92448.1"
                     /translation="MKKALMLIVLMAATAAIFAQAVDLFFSEYVEGTSNNKALEIFNG
                     TGDPVDLSNYTVKLASNGGTWSTTNILHMTGVLDDGEVYVIANSGAEASILAIANVTS
                     TVTYYNGNDVVGLFNGDTLIDIIGVYQQDPGPANWPVAGTPSGTAEHTLIRKPDVIQG
                     NTNFIAGAGTNQDDSEWIVHPQNYFADLGQHTFNPSAPEQAATPVFNPPAGVYANPIN
                     VTITSETTGAEIRYTTDGSEPSESSTLYTVPIAISSTTTLKAKAFAAGFNPSYTATAN
                     YLFPVQISNIAALR"
BASE COUNT         4719 a         2636 c         2575 g         4673 t
ORIGIN      
        1 gatactatat actaaacaaa gatatacaag acgagttggc atccctctta tatttttgcg
       61 cgaagctaag aagaaagaac cagctaatgc tcctactccc atagccgaca ttaataaacc
      121 ctgagtagca gaagtgccct gtaaaatatc cttagcgaaa ataggcatta aagtagaaaa
      181 agacattcca aaaattgtat atattgccaa attgcttatt aaaaaccgaa tgggcatatt
      241 ctcccacgca tatttccaac cagaaactat ttttttgaga gtaggttctt tttgaggaga
      301 aggtggggga tagcttattc taataaagca caaggaaatt attactggaa tatatgaaat
      361 agcattaata gcaaaacata ccccttcact aaacatcata attagaaaac caccaattgc
      421 aggaccaatt aaacgcgctc cattaaacat agcagaatta gttgctatcg cattgggcaa
      481 taaccgtctg gaagaaacaa gatccattac gaaattttga cgaatagggg catctatagc
      541 atcaataatt ccttgaagca aagataatat tataatagga tactgaacct gcttgttgat
      601 tactccggtc aaaactaata tagccagaat ccctgtttgt aagcagaaag caatttgagt
      661 gtatatcatt atatgatggc ggtttacacg atctgcccaa gcaccagcaa aaggactgac
      721 aaaaacagat ggcaccattg aaagaaaact cactaaacca agcaaaaatg cagaaccggt
      781 taaacgatat acaaaccagc ccatcgtagt tcgttgaatc caggttccaa ttaaggaaat
      841 cccctgtccc ataaaaaaga gacgatagtt tctaatagtt aatgaaacga agatttcttt
      901 tagttttgaa atgatcattg ctataggata aagcgggaaa ggtcctcact ttcgatgaca
      961 gggttaagat attcagttac catctttttt gtgattttaa cgcttttaag agtttcggaa
     1021 ggcatttcaa atagaaaatc atcgagcagt gtattcatta cagtatgtaa acgacgagct
     1081 ccgatatcct ccattttttc atttgctaaa gctgcaaaac gagcaatttc tcgaattgca
     1141 cttagcataa atgttagctc gactccttcg cttcggaaca tctcttgata ctgttttgtt
     1201 aatgaatttt ctggctcaat aagaatgcgt tcaaaatctt ccttagttaa actattgagt
     1261 tccactctta taggaaatct gccttgtaat tccggaatta aatctgaagg tttagctgaa
     1321 tgaaaagcac ctgcagcaat aaatagaata tgagaagtat caatcattcc atatttagta
     1381 ggaactttac taccctcaac aatgggaaga agatctcgtt gtactccact acgagaaaca
     1441 tctaatccac cttgcttatc aatattggca attttatcaa tttcgtctat aaagataatc
     1501 ccattttctt ccactgaatt ttttgctgtt tcaattagtt tatctttact tatcaaccgg
     1561 ttaatttcgg tttcaatata tcgtttccag gcagtgtgaa cagtggtttt aattgttctg
     1621 gatgcacctt taccctgaaa gatattagtc aacatttcgg taaaatcaag atctaataat
     1681 tctaaacctg gtcctggtaa tatttccaga ttgggagtag tgtctaattc gggttcaatt
     1741 tcaatttctt tatcatccaa actacctgat aacagctttt tacgcatttt ttcccgactg
     1801 cgtaaatacc tatcatattc tgcattatca ctaacgcgat tttgtttcat ttttttgctg
     1861 cggggaagca aaatatccag gatcatttca gttgcattct tttctgcttc aggacgaact
     1921 tcttctatca tcatctcgcg aatctggcta actgcataat tcattagttc acggaccatc
     1981 gattctacat cgcgtccaac ataaccaacc tcagtaaatt tggatgcttc tactttgaca
     2041 aaaggagctc ctgtcaatcg tgaaattctt cgagcaattt cagtttttcc tactccagta
     2101 gggcctatca gtataatgtt attgggaata atctccccct tgatatcacc agatatttgt
     2161 tggcgacgcc aacgatttct aagagcaata gctacacaac gttttgcctg atcctgccct
     2221 attatatatt tatccagttc ttcaacaatc cgttttgggg ttaaatgtac atagtttaac
     2281 aatttctaaa cttcctctac aatatagtta ctatttgtat aaatgcatat ttcagaagca
     2341 atatttatag cttcaacagc aatttcacca gcacctaatt ttgtttttct agctaaagcg
     2401 cgagcagctg ccaaagcata atttccgcca ctaccaatag ctattatatt atcatcaggt
     2461 tcaagcacat ccccaactcc attaattaga agaagggtat ctttatctcc agcaattagc
     2521 attgcttcca accgtcttaa tattctatct tgtcgccagt ccctggctaa gtcaattgct
     2581 gctttgcgta aattaccttt attggatttt aatttatcct caaatctttc caataaactg
     2641 aatgcatcag ctgtagctcc cgcaaagcca acaatcactt ttccatcaaa aattcttcgt
     2701 attttttggg cattgccttt tacaatagct tcacccatag taacttgtcc gtctccgcat
     2761 atagcagttt taccgttgcg gcggatacct ataatagtag ttccatgcat aatcataatt
     2821 atgaagtttc ctcaagtttt gtatctgtgt cttcggtttt atcttctaat gtttcaggtt
     2881 cagaaactct agctttctcg ccaggttcaa agcgaagttc aagagctttt ttatatttgt
     2941 tttgtacaat ttcagattca tcatcgcggc aattatcaag aataagctta aatatttcat
     3001 cagtgcctaa agtttcttta tcaaaaagtt cattagccat aatttccatc agttctttat
     3061 gctcattcaa gattgaaata gcttgagaat aggcattatt gatgatttct ctaatttcac
     3121 gatcaaccat ttgtgaggtt tcatcactat atatatcacg ggaaacaagt tcctttccca
     3181 gaaacacttc tccctgttct tttccaatag tgataggacc aattttatca ctcattcccc
     3241 aagaacagac catttttttt gctatatctg tgctgcgttc caaatcattt ccagaaccag
     3301 tagtaaattc tgtaaaaata acttcttcag ctgctcttcc acctaacagg gtaactaata
     3361 attgaagtaa atagctcttg gaataattgg ttttatcgct taaaagataa tgagttgctc
     3421 caccagtaaa acctctcgga ataatggaga ccttatgcac agcttcaact ttatccaaga
     3481 atacagaggt gagcacatgg ccaatttcat gataagcggt aaggcgtttg tcttcttcag
     3541 gtataactct gcttttcttt tctttaccta gaataagctt atctttggct tcttcaaaat
     3601 catccatttg aatctgagtc ttgtttttgc tggcagcaat caaagcggct tcattaacca
     3661 gattagctaa atctgctcca ctaaatccag gagttcctct tgaaattatc tccaagtgta
     3721 catcattagc aagcggaact ttagcagtat gaactttcaa gatttccgta cgccctttaa
     3781 tatcaggtaa atcgactgta actcttcgat caaatcttcc aggacgcagt aaagcaggat
     3841 caagaatatc aggacgatta gtggcagcaa taataattac tgcttcattc ggctcaaaac
     3901 catccatctc tactaaaagt tgattcaagg tttgttcacg ttcatcatga ccacctccta
     3961 aaccacttcc gcgatgtcta ccaactgcat ctatttcatc gataaaagta atacaaggag
     4021 catttttctt tgcttgttca aacaggtcac gaactcttgc agcaccgaca ccaacaaaca
     4081 tttctacaaa atccgaacca ctgattgaaa agaagggaac tccagcttcc ccagatacag
     4141 cttttgccaa aagagtcttt cctgttccag gccgtccgat aagcaaaact ccccgcggaa
     4201 ttcttcctcc taatcgttga aactttttag gatccttcaa aaattctaca atttcctgca
     4261 attcttcttt agcttcatca actccggaaa catctttaaa agtaactccc gttttactcg
     4321 cttcgtataa tctggcttta cttttcccaa aactgaatgc cttagcattt tgagcattca
     4381 tcccacgcaa gaaaaatgcc cagaaaatta ttaacaataa aaacggtaat agataggata
     4441 ttatacttgc ccaacgcgat ggttttgtgg cgctgacttt aattcccatt gctacaagag
     4501 aatctacaag ctggggattt tcgaaaggca gggtagtggt atatttctta ttggcaatat
     4561 cagtgtaaaa aatgtccttt tctgaaaact gtacctgcgt tatttggcca ttagcaacgc
     4621 gtgccataaa attagtatag ctctctttag ttatagaagt accgcttgta ctaaatatat
     4681 gaaagatgat tattaccata aggatgagca taataatcat aggtaaagag aaagcagatt
     4741 ttttgggagg tacatttttg gggggttgag gatttcgaaa atccggattc ggtttatctt
     4801 tgttcttaat atttcgattt ctaacaatta tggatctgat aatactgatt atcgaaatta
     4861 ataataaagc cagaataaac cagttcataa gtgtggaaaa ttgcataaaa gcatttcctg
     4921 cctcagggag agttttctga ggcacagtta atgtgtctgc agttgtagca tttaaaaaac
     4981 ctaatgctat tatgcagaat agaataagta tgtatttcat catttatata gctcttcttt
     5041 taatattcct atatagggta agttacggta tctctctgca aaatcaagtc cgtagccaac
     5101 cacaaattcg tttttaatgg aaaaaccgat gtattcaaaa catacatcta ttttatgggc
     5161 atcgggttta tctaaaagta cacaggtttt caaactggca ggtttatgtt gccagatata
     5221 atccttgata tattctaaag aaagacccgt atctacaata tcttcaacta taattacatc
     5281 tctgttagac aaatcaatat cgatatcctt gcgaattttt acgattccac tactattagc
     5341 atcattacca tagctggaaa tagctaaaaa atcaacttct acagggatag taatactccg
     5401 gactaaatca gccaaaaaga taaatccccc ttttaaaata cctaccataa cgggtgtact
     5461 atctttatag ctgttggata tttccaagcc gagttcacga attcgagttt gaattttgtg
     5521 ttcgtcaaag agaacagcag aaatatcaca attcatctta ttcatccttt cctcttttct
     5581 tttttatgga ttgcccttct ggattttgaa gcaatagggt ttgccactat ttgcaaatag
     5641 cgagaagtgg tttcatcaca ttttacccga tcatccaaac ggcatcctac aatccaaaat
     5701 aacttttctc catcttctaa aatgggtatt aaatccctct catatttagg gactttttcg
     5761 tcaataaaaa actctttaag acttttatat tgtgtcatcc caaaaggcat aaaccgatca
     5821 ccgggttttc tgaatctgat cataaaaggg aaattcactt tatcggcatc aataataatt
     5881 tgttcttgac catctgccag cttatgatct ttgggttgta ccttcaaata tttgaaactg
     5941 aaacgataat tgccataaac agccatagta cggtctgtat ctatgatcat aggttctggt
     6001 tctgtatttt ttttaatatc tccaacttgg ataattaact cctcatattg ttttataacc
     6061 acgatatttt tgggaagata gatacattta ctgcctttag tagcaagaat attatctatc
     6121 gcctgatagt gaactgcaaa gaaatcatta tcatcatcgc agaccttgat aaagacattg
     6181 cgaagtaaat aatacctttc aattttgcta agctttataa gtagtgggca agataaaact
     6241 gttctttcgg gttgtatatc taaacaaatt cgtttatatt gagactcact gcgctgtttt
     6301 aaaattttat ctgcctgagc aaaaagttcg gctacaaatg agagatgtga agataacata
     6361 ggattaaatt ctctttcaag acgaggaata agttcattgc gtaaaagatt tctacggaat
     6421 ttattgtcca aattactgga atcggtcctc caggtaatat ccactttaga taaaatatcc
     6481 tcaagttctt ttctactaaa acaaagcatt ggatgaacaa ttttaccaga aatagcttta
     6541 atccctgcca taccagtaat tcctgaacca cgaaaaagat ttagtaaaac tgtttctgct
     6601 tgatcttctc tttgatgagc aagcagaatt ttatcaaatt tataactatc cagcactttc
     6661 gcaaaaactg aaaaccgcag tttacgagca tggttttcca aatttcctgt tttaggaact
     6721 tttactttat gaataattaa ggggatattc aaattggaac atagttcttt tacagattcc
     6781 tgatcagcat cactttcagc tccgcgaatc tgatgattga catgcactgc taaaagagtt
     6841 aaattataag taactctcag ctgagaaaaa agatagagca tagcattaga atctgcacca
     6901 cctgaacatc caagtaacaa cttatcacct atcttaataa gctcattttc attgatgttt
     6961 ttttccagac gctgaagggc ttgggcaatt tccatcttga ttcctcatct cataatattc
     7021 ctattttcta ttattttgat ataggtttga gtgtcaatag gtttttttac agaaaattgc
     7081 ttattcgttt aacaattata attgtataaa ttacgaggtt tatatgctat gattgatgaa
     7141 cctatagtga atacaggcaa atcagttata ctaaattata cttgcagtgg gttcttgcta
     7201 tgaatccttt aagaataaca ttattgtagg gttttgtgtg tttattgctg ataccaaaca
     7261 tactatttcc ttctgtgctt gatttcgtat attttggaag ctatattaaa gattcaatac
     7321 ttgttttaaa attctcttat ttcatcaagg aaatgctaga ggatccctaa agaatctccc
     7381 aagaagatat cagttacata acaataaaat cagattattc aacgggctta ttatagggga
     7441 tttcctattc attatctaat gtctaatggt ttccgacaat cttattgata gttggattat
     7501 tggttaaaaa cgagtaaaaa aacttgacga ataatcaaat aaacacgaaa aggaattata
     7561 attaattaat tgagcgagga aacgatgcag gcagacctaa ttattattaa tattagcaag
     7621 ttggttactc ttgcccataa tggccgcccc cgtttgggaa aagaaatgga agagacagct
     7681 acaattttaa ataaagcact ggctgtaaaa gatggtaaaa tcttagaaat aaacgatagt
     7741 aagtcaattg aagaaaaata tagaaatgta gaatgtattg atgcaaatgg acaaatagct
     7801 acacctgggt tcgttgattg ccacactcat cccatttttg ttcatacacg agaagatgag
     7861 tttgcaatgc gtatacaggg gaagacttat gttgaaattt ctaaggctgg aggtggcatt
     7921 ctgaatacta ttcagacaac gcgtgatgct gatgaggatt tactttttga actagccaaa
     7981 aggcgtattt taaaaatgat ccaacaaggt actactactc tagaagcaaa aagtggttat
     8041 gggttggata ctgaaaatga actaaagcaa ttaagagtaa ttagacgctt acaggaagaa
     8101 cttctgattg atattgtgcc cacttttatg ggagctcatg aatttcctcc tgaatataga
     8161 aataatcatt ctggttatgt agaactactt tgtaaagaaa tgattcctgc tgttgcggaa
     8221 cagggaatag ctgaattttg tgatgttttt accgaagcac atacatttaa tattgaagaa
     8281 tcgcgtaaaa tacttagttg tgctgctgaa tatggattaa aactaaaaat gcatagtgat
     8341 gaaattgaat caattggcgg tgcagaactt gctagtgaaa tgaaatgtat atctgctgat
     8401 catttaggtt cagctagtga aacaggaatt aaggctctac aaaaagccgg tgtagtacca
     8461 gttttgttac ctgccacttt attttcctta cagagtaaaa aatacgccaa tgcgaaattg
     8521 atgatggaat taaatcttcc tgttgctata gctactgatt ttaatcccgg tagctgtaat
     8581 tgtgatagta tgtccttaac aatgtctctt gcctgtttac aaatggggat gactcctgcc
     8641 gcgtctttat gtgcagcaac tttaaatgct gcttttgctt tagatagagg caaaataatt
     8701 ggctctctgg aaactggtaa acaggcagat atagtacttt gggatattcc cgatttgaat
     8761 tttatccctt atcatttagg atcttcttat ataaccagcg tgctaaaaaa tgggaaaata
     8821 gtgtacaagg caacctaaat ggctgataca tctctaaata gctatcaaat aattaaaact
     8881 gtaaacagct ctttgcgctt ttctctctat caggcaaaac agatagaaac aggaaatgta
     8941 gtcctaatta aaactccaga tagccaaaga acaaatgaca taggattaaa acacgatcta
     9001 gttaatgaag ctaatagcca tttaaaacta aatcatcctt tcatcagaac tgtttatgaa
     9061 acaagagaag aaaaaggaac tgcctatcta attggcgagt atattgaagg aagatctctc
     9121 tctgcttatc tgaaaaataa ccctaattca tttactttgg aagaatcact ttctcggttg
     9181 caagaacttt tggaagccat ctcttacgct cacaaaatgg gttgtattca tttgaattta
     9241 aatccgtata atatccttgt tgataaggaa aaccatctgc atattattgg tttcggtaaa
     9301 aaccaagatg cttataaaac cgcttacgaa aaagatgaga cctatcatcc actattgtac
     9361 attgcacctg aactgtattc ggcgggaatt gcccttcctc aatccgattt atattctttt
     9421 gctgtaattg cttatcatat actatgtggt gtcattccct ggcgggtaga caatcaactt
     9481 attcctgaaa aacaaaaaca gcagtcactt tgccgagctg ttattatgcc agagatttta
     9541 aataaacaag ttccggattg gctttttgca gctttgttgc aatgtttgaa acttgatcct
     9601 catctgcggt tttcaaatgc agaagaactt ttggaacttt ttactaaaag ggataattgg
     9661 gttcccgaac aggaagcaga gataattaaa gaagtagaaa aggttgaaac cattgtagac
     9721 atagagaagt gcgaagcgat tgagaagata gaacaaattg aagatgttca attagttgat
     9781 gatttgccac ctacaaattt ggcattaaat gtggattcac agcaaaaaat taatacagat
     9841 atctcacatc tctatggaaa attggatgtt actatcccag aagaaatacc tcaatccgat
     9901 tcagtaaaaa caatatcgga tactactccc aaagatactc ctaaatattc tttcactact
     9961 tctgcaccgg tagaaactat tgaaaccaga aaactgaaaa aaacctttaa agtccttatt
    10021 tggatgtctg ctattgtcct actattcatt attattaaat atgtagcttt tggtaaccgt
    10081 cccaaattta cttctgtagc agatagcacg caagtggaaa tcccagaagg acctcttaat
    10141 aaaaacctac ctattgcaat gatgttagtt cctgccgata ctttagtgat gggaaatatt
    10201 gctcccgagg cagatgatga tgaatttcct cttttaacta ttagtattcc agctttttat
    10261 atcggccaaa aagaaatcag tcaggcagaa tggatgatgg tttttcccaa taatccgtct
    10321 catagtaaag atcctgcatt acctgtggaa aatgttagtt tttacgatat tatagaattt
    10381 tgcaatcaga agagtgtttt agacggtttt gaaccttgct acgattatta cgataatgaa
    10441 gtggtttgca atttttcatc aaatggttat cgcttgccca ctgaggcgga atgggaattt
    10501 gccgccaaaa gtgggaaaag atatgatttt tttgtttaca gcggttccgc tgatcctgat
    10561 gaagttggtt ggtataatag caatagcgaa gctcaaagtc atcctggagg acaaaaaaaa
    10621 cctaaccaac tgggaattta tgatctaagc ggaaacctgt ttgaatgggt ctggaattgg
    10681 tatgctcctt attcagcacg caactggaat ttgataactg gtccagataa aggaaccgat
    10741 aaagttatta gaggtggttc ttggtatcat agtgtttccg aaatgagagt tactaaccga
    10801 aattatgcta aaccttacac gaaaaatgcc taccttggtt ttcgagtagt aagaacaaaa
    10861 tcttaacaag ttcaaagttc agagttcaaa gttcaaagtt caaagttcag agttcaaagt
    10921 tcaaaagttc aaggtttaaa gttcaaagtt atatttggca aaaccgtgca attcttacaa
    10981 ttaaactctc gctgcgttag ctctcattac ttcatttcta cttgacagca aacggcttac
    11041 cgatgtttag gttataaaaa taaaataaga ggaaaaacca agtggaatac actgattata
    11101 aaaaaacagc caatgaaata attgaacaaa tatctgaaat tcggaggtta ctttgatccg
    11161 gagccgaaga aattattaat ctccgaatta gagcaaaaga tgaatgctcc ggatttttgg
    11221 ttagatcaaa caaatgctaa aaaagtaagt aaacagcttt ctcaactacg agacgaacta
    11281 gatcacataa gaaaaatgga aaatataaaa ggtgaactgg aaacttatct ttctttgctg
    11341 gaagaggatt ttaatattga tttactaaat gaagcagttt cggaacttcc acgcatacag
    11401 aatttcatag agaaagcgga aattgaatgc tacctgaatg ataaatatga tcataatgat
    11461 gctctgctaa ctattcattc cggtgcggga ggaacagaat ctcaagattg ggtggaaatg
    11521 cttctaagaa tgtattctca ttgggcagaa aagaataatt atagttttaa tattattgat
    11581 tacctaccag gagaagaagc tggaataaaa agtgtaagtg ttgaaattaa aggtgatttt
    11641 gcttatggaa tgttgaaaag tgaaataggc attcatcgct tggtaagaat aagtcctttc
    11701 aacgctcaag gcaaaagaca gacctccttt gcctctgttt ttgtatatcc tgaatttgat
    11761 gaagacctgg atgtggaaat agatcctaaa gacttgaaaa ttgatacttt ccgctcaagt
    11821 ggagcaggag gtcaatatgt taacacaact gattctgcag tacgcattac tcatttacct
    11881 accaatatcg tagtaacttg ccagaatgaa cgcagtcaga ttcaaaacag agaaaaagca
    11941 atgtccattc tgaaaagccg cttatatcaa tactatgaag aacaacggga aaaagaaaag
    12001 cagaatgtag agagtagtaa aacagaaatt ggatggggga atcaaattcg cagttatgtc
    12061 ttccaaccat atcagatgat taaagatcat agaactaatt atgaaacggg taatactgat
    12121 aaagttatgg atggtgattt gaatgatttt atctacgctt ggttgaaata taatgccaaa
    12181 cagaggataa atggctaaac aggattataa acaattgctc aacgaactgg atagggacaa
    12241 attacctaag catatcggtt ttattatgga tggtaatggc cgttgggcaa aaaaaagaaa
    12301 taaacctcat ctttatggtc atagagccgg tgtaaagtca ttgcgtcaag ttgtagaatt
    12361 gggagttgag ctaaaacttc aatatttaac cttctatgct ttttctacag agaactggag
    12421 ccgtccagaa agtgaagtaa aaggcctttt atggttacta aaagaccagc tgaaaaaaga
    12481 aattccggaa ctgatggaac aaaatatcta tgcacagttt attggaactt ctattggact
    12541 ggaaaaaaat tactggaatg aagtaagttc gcttgtggct caaactcata agaatacagg
    12601 aatgatagtg aattttgcct tcaattatgg aggtcgtctg gaaataattg agggcttcaa
    12661 aaagtttatg tcaatgaacc cggacaaatg ggataaattg actcccgagg actttggaaa
    12721 ttatctttgg acagcaggac aaccggatcc tgatttgata attagaacca gcggagaaaa
    12781 gagactttcc aattttttgc tatggcaatc agcttatgcc gaaatataca taacagagac
    12841 cttgtggcct gattttgata aaacagaaat gattaaagca cttttagatt atgcctcgcg
    12901 tgaccgtaga tttggaggta gaaaagaatg aatgagttat tcaaaagagt gcttgttgct
    12961 gctttattaa ttcctgtagt ccttattatt ctttattttc agggcttatc tctagttatc
    13021 accttagcaa ttatagtttt tttgggaggg ttggaatata taaggatgat gcataaagca
    13081 aaaatacaaa taagttatgt ctggattgtg ttaaattgct tgttctattt tgctattgta
    13141 tatataaagg gcagagattt ctctttgtta tggttagctt tactttttgc tctaatagaa
    13201 tctattacta cttggaacgc agagcgttct attccacgac tttttgctac ttttttctgt
    13261 tttgtatata ctgctctatt tcctgcttta atagctcgta tcagtttatc ttattcagaa
    13321 tataattttt tacttgcctt aattctgatg atatggtttg tggattcaat agcttatttt
    13381 gttggtatga aaataggaaa acacagaaat ataacagcaa tcagtccgca aaagtcctta
    13441 gaaggttttc ttgcaggaat tcttgcgccc tggttgattt tgattatatt atatatttgc
    13501 aaggttagaa ttatgtcttt ttcctatctg gctttaattg ctattgcagc aggcattatt
    13561 ggtcagattg gagatctgat tgaatctatg ttaaagcgtt attgtaaagt gaaaaatagt
    13621 tcgaatctgc ttcccggtca cgggggaatt ttagatcgtt gtgatagtat tctattcgca
    13681 ggttcgttct tatattgtgc tttggaaatt ttaacaaaag tgaggtaata ataatgaaaa
    13741 aagcacttat gctaatcgtc ttaatggcag caactgcagc tatatttgct caagctgttg
    13801 atctcttctt ttctgaatat gtggaaggga caagtaacaa caaagcatta gagatcttca
    13861 atggaactgg tgatcctgta gatttatcta actatacagt taaacttgct tccaatggag
    13921 gaacctggag tactaccaat attttgcata tgactggtgt tttagatgat ggtgaagttt
    13981 atgtgattgc taattcagga gccgaagctt ctattcttgc tatagcgaat gtgacttcta
    14041 cagttactta ttataatggc aatgatgtag taggtctttt taatggtgat actctaattg
    14101 atatcattgg tgtctatcaa caggatcccg gacctgctaa ttggcctgta gctggaactc
    14161 cttccggtac tgctgaacat actttaatcc gtaaaccaga tgttatacaa ggcaatacta
    14221 attttattgc tggtgcagga acaaatcaag atgattctga atggatagtt catccgcaga
    14281 actattttgc tgacttgggc caacatactt ttaaccctag tgcacccgaa caagctgcta
    14341 ctccagtttt taatcctcct gcaggggtct atgctaatcc tataaatgta actattactt
    14401 ctgaaactac aggagctgaa atacgttata caactgatgg tagtgaacca agtgaatcat
    14461 caaccctcta tactgttcca atagctattt catctacaac aactttgaaa gctaaggcat
    14521 ttgcagctgg ttttaatcct tcttatacag ctaccgctaa ttacctattt ccagttcaaa
    14581 tatcgaatat agcagccctt aga
//
LOCUS       DAORHB010000010        14444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3250_length_14444_cov_5.099103, whole genome shotgun sequence.
ACCESSION   DAORHB010000010 DAORHB010000000
VERSION     DAORHB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14444
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3250_length_14444_cov_5.099103"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKM80_00670"
     CDS             <1..302
                     /locus_tag="PKM80_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92449.1"
                     /translation="TLGSWDSCFALPQNPLLMNYELGILRTREHRFEFSFRCKTELGK
                     SASNTIQKISPVIELVIDNCVNDNVAIIHEVFLLRSTFIYYSVSLSLGLLGKISN"
     gene            complement(282..1013)
                     /locus_tag="PKM80_00675"
     CDS             complement(282..1013)
                     /locus_tag="PKM80_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNV92450.1"
                     /translation="MTKKNALHPLTHILMALLLSTMVFIVKKPSSLLFLTLLASIYAA
                     FRMKNGWQKILKTLWHTLPFLLVLSFFQIVFRRSGSLLWSYGILKISSEAVQISIQLA
                     LRLLTVIYCAKSLAVMEYPEFSKAFSAIRFPEELSFMLSYAIHLVPKFLAQLKGFVTG
                     LKLRGIDSANLSIAKRLQVYKQLAITSLAELIRNSETSAIALELRGFRSEGKRTKLYQ
                     QKFSYRDILFLLVFSGIVVLVTNLS"
     gene            complement(1010..2359)
                     /locus_tag="PKM80_00680"
     CDS             complement(1010..2359)
                     /locus_tag="PKM80_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV92451.1"
                     /translation="MLILKDFGISFADRNLLEGINIEIPEGTVFHLKGPNASGKSSLL
                     NAISGIIPEYVQAKTTGDLIWNNIDLQTIPLKEKHHYLWHQLSDTEAQIFFPDCISEL
                     AFALENMAIPAEKIWERVNLATQQFGLSNLLYRDPSTLSGGEKKLLLCAIAETIDPPI
                     LLLDEPLNGLDNRAVKLVLKWISDKKEQGKIIVVVDHNPIIETLADFSFILADNPQLP
                     KTDIAFISNNYLNIADNLGYQINPNTDILYRVEELDFAYPQSKPLFTKLTVEIGSKQN
                     ILLTGNNGAGKSSFLKLLAGLIKPLKGKIYLGGKLMQGLDTKNFEYIYYQSQITKENL
                     LGISVAQNWLFWQLAIKQLPDLPLKDDPLFTELSAGQQKMVSQQILPQLLSKFWILDE
                     PFASLDKDAGTKLLQLLQYKSKKHPGMLIVSHSLEEHNDMFDRVLTIRNHFLQEETK"
     gene            complement(2359..3285)
                     /locus_tag="PKM80_00685"
     CDS             complement(2359..3285)
                     /locus_tag="PKM80_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92452.1"
                     /translation="MKKYICLLILLEVIVFLTALEIIDSKGISHIYDNAELYKLETQE
                     LKTTREKDGIVRLNNWQGFRFDIWLKQQKLGDFATIRFESSDRYLVTLEKSEFDSLES
                     YIVIAQDGIPFEEYMLRLIFPTLREMQWIKGLDRVVLENFHPLIRPTRFFLLQDTLKK
                     YTLQKDLKPFVNIEGYYLSDVLMDLSPAEEKKVILYSRDGLKQSLTYPLHLQGAVLEK
                     TPEDLYNLKSPQIPGGMWMKDLVYLQCDNYALISEDSINQLIRLAKILHWETTPEMQF
                     RIVYKDSEEIMNFTDALAEPQVFRGALYFEIF"
     gene            complement(3285..3821)
                     /locus_tag="PKM80_00690"
     CDS             complement(3285..3821)
                     /locus_tag="PKM80_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNV92453.1"
                     /translation="MKLLQHFTVKDLIIITSIAALGIAVKPIINPITKIISTPLGIPG
                     GSLTGGLYMLWLVLTIVIVDKPLTGTLYGFLQSILVLLIGMAGRMGAFSLVAYTLPGI
                     IADLIYWLYPKKNKLLTHLLLCAFANLTGSLVSSAIFFKMPPLMIGFNTCLALASGII
                     GGYLGFGIYQSLKATRII"
     gene            complement(3821..4324)
                     /locus_tag="PKM80_00695"
     CDS             complement(3821..4324)
                     /locus_tag="PKM80_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNV92454.1"
                     /translation="MKNTKSTGEKMERPSWQQYFMEMAYLAAKRSTCLRRKVGAVLVR
                     DNQIISTGYNGSPKGVPHCAEIGCLRKQQNVPSGKNHELCRGVHAEQNAIIQAAINGS
                     STRGSILYCTNQPCSICARLIINAEIKTVYIAETYPDELGEELFREAGIELIQYELKS
                     NTLKKLI"
     gene            4894..7650
                     /gene="polA"
                     /locus_tag="PKM80_00700"
     CDS             4894..7650
                     /gene="polA"
                     /locus_tag="PKM80_00700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNV92455.1"
                     /translation="MADKLYLIDGTALLYRAHFAFIKNPLINSKGKNTSAIFGVINSF
                     LHIIENTQAAYVAISFDRKAPTFRHTLYEAYKAQRPPMPEELTAQIEPVKKFFELIGL
                     KEIGMDGYEADDILGTMAKQFKDQFQIVFLTSDKDYCQLIDERSVMLDPMKDVILDRD
                     AVYAKYGVYPEQFIDYLALVGDPSDNIPGVKSIGPKTASMLLSEYKTLDNIYAHLDFI
                     KPRWKELLLENKENAYLSKELATIKTDVPIPKPDLEYLTFWTKDLKKAIPLLEEYEIN
                     SIKRKIELNYPEPTDTIQQSEYQDDIFNMESKKSEAVSEADTKMPFKAILVNTRNFVT
                     LLKEIKEAQLISLDTETDSPDPMLANLAGISFCMNEAKAYYLPLGHQMADNLSLPEVI
                     RHLEDAIQGKLLLGHNLKFDLIVLCRHGLNLNNPIFDTMLAAYILDPGTLNYSLDACA
                     VSELNYKMIPISDLIGKGKNQSTFDYVELDRACIYSAEDAWVVYKLYPIYHRKVDFSV
                     MANLFYTIELPLIKVLQRMEMNGMAIDCSVLTEISHKINQELKKLTDIIYAYAGYEFN
                     LNSTQQLAKMLFEEKKLPTRKKTKTGYSTDNSVLEELAEDYEIAGTIIQYRQLAKLQS
                     TYINALPKMINPETGRIHSSFNQTVTSTGRLSSSNPNLQNIPVRTEIGREIRKAFYAK
                     DEEHLILDADYSQIELRLLALMSEDEVLIEAFKQDLDIHKRMAAKIYNVSLEQVTPDQ
                     RRAAKTINFGILYGMGQRKLSRELGIPQNEAKQIIDDYYAQFPSIREFINQCILKARQ
                     QHYAETLFGRRLYLPNIESKNQGLRSEAERIAVNMPIQGTAADLIKIAMLDIHNQIDK
                     REDIKMILQVHDELLFEVHKKAIEEAKIIVQTCMEHALPKEYADKVHLKTDLGVGVNW
                     FEAH"
     gene            complement(7750..8475)
                     /locus_tag="PKM80_00705"
     CDS             complement(7750..8475)
                     /locus_tag="PKM80_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92456.1"
                     /translation="MKRLIMLLLALLVLAGCVQYDEELWINRNGSGRAVIRIVHRSPY
                     ENPEEIMRKAALPGINLQSYDIYRKGPDVIYTINFKFKNIDAFNNVNDQLGTADFWGK
                     ITLNKEPGRRITFKRRIALGSQEPEDEDDIEHIIGRLQPDNPVWTYKVHLPWKIISTN
                     ALPENVDYNNRTVAWKYDTRKMWHKQELMSVELRKDFPWLLLVIGVIVLILLTFLVHW
                     ILRFPKKSHWLERIKHTEDAEKK"
     gene            8617..8994
                     /locus_tag="PKM80_00710"
     CDS             8617..8994
                     /locus_tag="PKM80_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNV92457.1"
                     /translation="MIALRQLWRCAICGNVVEVTYVGGGELVCCGQPMYLLQENTVDA
                     ATEKHLPVVKDLGDKIEVSVGSVPHPMEEKHYIVFIEVLTDKKVLRKELKPGEEPKAI
                     FKVSMDKVKEVREYCNIHGLWKA"
     gene            9015..9605
                     /locus_tag="PKM80_00715"
     CDS             9015..9605
                     /locus_tag="PKM80_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNV92458.1"
                     /translation="MSFRETRTAENLMKSFAGESQARMRYLYSAKTAKKEGFEQISNI
                     FTETADNEKEHAKVFFKHLLSNGVEGEMINIIASYPVGWSADNTLKNLEYAANGENEE
                     WSELYPLFADVAEEEGFSDIAHSWRMIAKVEREHEKRFRKLYDNVKNQKVFIRQEKVL
                     WKCLNCGYVHEGTEAPKICPSCSHPQSYFELFKENY"
     gene            9626..9766
                     /locus_tag="PKM80_00720"
     CDS             9626..9766
                     /locus_tag="PKM80_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNV92459.1"
                     /translation="MQKYQCMACGWIYDPAENNNIPFEELPDDFVCPECGVGKDIFEP
                     YE"
     gene            9846..10298
                     /locus_tag="PKM80_00725"
     CDS             9846..10298
                     /locus_tag="PKM80_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNV92460.1"
                     /translation="MQQQEFEEILDFAISREEEAVKFYQELQEQSQFQDVKNMLKDLE
                     MMENGHIQIIENIRITGVKEEDIQKVPNLKISEYLCVDPSDLDMSYQNLLVRAMKREE
                     NSFLLYSEMSVKFPDAEIATLFRRLAADEAKHKLFFENLYDDWIRTGN"
     gene            10277..10969
                     /gene="tsaB"
                     /locus_tag="PKM80_00730"
     CDS             10277..10969
                     /gene="tsaB"
                     /locus_tag="PKM80_00730"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNV92461.1"
                     /translation="MDQDWKLKLAIDSTQNSGSIALANEERVIYSAYFDIKITHSETL
                     MPAIDYAFKFCNILRSELQEIYVCIGPGSFTGLRIGLATAKGIAFALGIPLYAYSSLE
                     LAALPASGLGKNIFAAVDAKMQEVYYAYYDQQLKEIIPPSIATPEELCNLDLEDFILC
                     GSASEMLALRFKNSYNFYTLHPVMKIPSAAGLFSIPNLLPEKHIPQILEELEPLYLRE
                     ARAQVKKAKPKQ"
     gene            11341..>14444
                     /locus_tag="PKM80_00735"
     CDS             11341..>14444
                     /locus_tag="PKM80_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNV92462.1"
                     /translation="MKKAFILLTAILIGINLAAFETVIKVDKNAWQTKQQADGYPVTL
                     QTGEPVLPYVPVNVLIPFGQRYQNASIQLAPAELVAERVTFEIAGVQLPISRMNIPNA
                     GSYSSAVLENKLYPEREWQFLGIQYFRGYQIALFNVYPYRYNPITQKLYVSSEISVSI
                     NSEFSEAEAEYQANFYTPNSKTLSYINSLICNPENIASYQSASNYRSVPILSRDIDLT
                     VPKQMIIITSNSRINWFTEYSSWRASKGVSNAIYALEDILSSYPGTDNAEKLRNFIIN
                     VYQTWANSSQPLEYVILGGDDEIIPERGTYGQVGDTIDERMPTDIYFSNLDGNWNANN
                     NNIWGEPADNPDMIPEVHIGRFPAETQNEFNHMFNKIRYYVDNDTYSNNYALFMGENL
                     NNNPLTWGGDYKDDVAQYLPDEYYLQTLYQRDGTYSSMNVYNAINKGAGIMNHMGHAN
                     ESVLIGQSNGTVESLTNTEYGFLYTQGCYPAAFDQRTSGDNESIGEHFVTASGGVFCF
                     IGNTRYGWYSPGSVNGASQFYDRQFFNGLFQQNLPVLGEALTYSRLQNLNAALSNDVM
                     RWCYYEVVLFGDPSIAVKSFNPNLPLLSLESYEITDEEGDNNGTISTGELICIYPVIS
                     NNTNWGTAYNVSVRLENLPEGIEVVGNNLSIAQILPGGMSPQNLKIRLQLSDTVGFGT
                     YYLTLVIDSINPVTNLSTGERRFTVSFEITLMDDRFPWETIINGKSAPLVTNLNSTSG
                     MEIIYVDVYGNSYYLGNDGEIYSTVEAPEGMYIFRSFACGPIDDDDENDLVFCSRSGD
                     IYAMHFDGEIIFNYHDNTMFLFTPVLADINGDGTLETIAGSLFGKIYAISPNGQLLNG
                     FPFDVGGNFQTELAAADFNGDGTMEIVAGSQNGLLYVLGTGGTVLPGFPVQLNGPVTG
                     SPTITNTNRIVCSTPTHIYIISAEGNIIAMRNIDANIAGGFALGDITSDDNGLDIVGI
                     TNNGIIYALTDEGVDLPGYPVATGDNFVCPPLIANLDSDPQAEILVHSYVNSVYAFNS
                     DGSTL"
BASE COUNT         4704 a         2534 c         2693 g         4513 t
ORIGIN      
        1 gaactttggg cagttgggac tcttgttttg ctcttcccca aaatccactt ttaatgaact
       61 acgaacttgg gatattacgc accagagaac acagatttga attttccttc cggtgtaaga
      121 cagagcttgg caaatcagca agcaatacga tacagaagat ttctccagtt atagaactcg
      181 ttattgataa ttgtgtaaat gataatgtag caataataca cgaggtgttt ttgttaagaa
      241 gtacttttat ctattattct gtctctctat cacttgggct actaggaaag attagtaact
      301 aatactacta tcccagaaaa aacaagtaga aataatatat cccgatagct gaacttttgt
      361 tgatatagtt ttgttctttt accttcactt ctaaaacctc taagttccaa agcaatggca
      421 gaagtctcac tatttctgat tagttctgct aaagaagtaa ttgccaattg tttataaacc
      481 tgtaagcgtt tggcaattga aagattggca gaatcaatgc cccgcagttt caatcctgtt
      541 acaaatcctt ttaattgggc taaaaatttc gggacaagat gaatagcata agacagcata
      601 aaagaaagct cttctggaaa acggattgca gaaaaggcct tggaaaattc tggatattcc
      661 attacggcta atgacttagc acaataaata actgttaata aacgtaatgc cagctggata
      721 gaaatttgaa ctgcttctga actaattttc aatatcccat aagaccaaag caaagatccg
      781 cttcttctaa aaacgatttg gaaaaaactt aagaccaaca aaaagggtaa agtatgccac
      841 agggttttta gtattttctg ccaaccattt ttcatcctaa aagcagcata aattgacgcc
      901 aacaaagtta aaaaaagtag cgagcttggc tttttaacga taaataccat tgtagatagc
      961 aataaagcca taagaatatg tgtaagtgga tgcagggcat tcttctttgt cattttgttt
     1021 cttcttgcaa aaaatggtta cgaatagtta ataccctatc aaacatatcg ttatgctctt
     1081 ccaacgaatg agatacaatt aacatcccag ggtgcttctt acttttatat tgcagcaatt
     1141 gtagaagttt agttccagca tccttatcta aagaagcaaa gggttcatcc aatatccaga
     1201 atttagatag taactgaggt aatatttgtt gcgataccat tttttgctgt ccagcagata
     1261 gttctgtaaa taatggatca tcttttagtg gtagatcggg tagctgttta atagccagtt
     1321 gccaaaacag ccaattttgt gcaacactaa ttcccaaaag gttttctttg gtgatctggc
     1381 tctgataata gatgtattca aagttctttg tgtccaatcc ctgcatcagc tttccaccta
     1441 aatatatttt accttttaag ggtttaatta gccctgctaa gagcttcaga aaagagcttt
     1501 tccctgctcc attattacca gttaatagta tattttgctt gctacctatc tcaacagtaa
     1561 gctttgtaaa tagcggcttc gattgaggat aagcaaaatc aagctcttct acccggtaaa
     1621 ggatatcagt gttaggattt atctggtaac ctaaattatc agctatattc aaatagttat
     1681 tactgatgaa ggcgatatct gttttaggga gttgcggatt atctgctaaa ataaaagaaa
     1741 aatcggctaa ggtttctatt atagggttgt gatctaccac tacaattatt ttgccttgtt
     1801 ctttcttgtc actaatccat tttaagacca atttaactgc ccgattatct aatccattta
     1861 ggggctcatc taaaagcagt attggtggat ctattgtttc agcaattgca caaagcaaca
     1921 gttttttttc gcctccgctt aaggttgaag gatccctata aagtaaatta ctaaggccaa
     1981 attgctgggt agcaagattt actctttccc atattttttc cgcggggata gccatatttt
     2041 ccaaagcaaa agcaagttct gaaatacaat caggaaaaaa gatttgagct tctgtatcgc
     2101 ttagttgatg ccatagatag tggtgttttt ctttcagggg aattgtttgc aagtctatat
     2161 tattccaaat aagatcaccg gtcgttttag cttgaacata ttcgggaata attccactga
     2221 tagcatttag cagagaactt tttccgctgg cgttaggccc ttttaaatga aatacagtcc
     2281 cctcaggaat ttctatattg atcccttcaa gcaagtttcg gtctgcaaac gaaatgccga
     2341 aatcctttaa gataagcatc aaaaaatctc gaagtaaagc gctcccctaa aaacttgagg
     2401 ttcagctaaa gcatcggtaa aattcattat ttcttcacta tctttatata caatgcgaaa
     2461 ttgcatctcc ggagtggttt cccaatgcag aatttttgcc agacgaatta gttgatttat
     2521 cgaatcttca ctaattagcg catagttatc acattgtaaa taaacaagat ctttcatcca
     2581 cattccaccc ggaatctgag gactttttaa gttatagaga tcctctggag ttttttccaa
     2641 aacggctccc tgcaagtgta atggataagt taaactctgt ttaagtccat ctcgactata
     2701 aagtataacc tttttttctt ctgctggcga taaatccatt aaaacatcac ttagataata
     2761 accctcaata ttcacgaagg gcttgaggtc cttttgtaaa gtgtattttt ttagagtatc
     2821 ttggagcagg aaaaaacgcg tcggacgaat aagcggatga aaattttcca atacaacacg
     2881 atctaaacct tttatccatt gcatttctcg caaagtaggg aaaataaggc gtagcatata
     2941 ttcctcaaaa ggaatgccat cttgagcaat aacaatgtag ctttccagag aatcaaactc
     3001 acttttttct aaagttacca gatagcggtc agaggattca aaacggatag tggcaaaatc
     3061 acctaacttc tgctgtttta gccagatatc aaacctaaaa ccttgccaat tattaaggcg
     3121 aacgattcca tccttctcgc gagtagtttt tagttcctgt gtttccagtt tatacagttc
     3181 tgcattatca taaatatggc taataccttt actatctatg atttctaaag cagttaagaa
     3241 gacaataact tccagtaaaa ttaaaagaca aatgtatttc ttcatcatat aatccttgtt
     3301 gctttcaatg attgataaat tccaaaacca agatagccac ctataattcc agaagctaat
     3361 gctaaacaag tattaaagcc aatcattaaa ggaggcattt taaaaaagat agcagaagaa
     3421 actaaagacc ccgttaaatt ggcaaaagcg cacaacaaca aatgagtaag cagtttattt
     3481 ttcttaggat atagccaata aatcaggtct gctataattc ctggaagtgt atatgccact
     3541 aaagaaaaag cccccattct acccgccatt ccaatcagta aaactagaat tgattgcaaa
     3601 aacccataaa gtgtaccagt taaaggttta tccactatta cgatcgttaa aactaaccat
     3661 aacatataaa gtccgcctgt tagtgaacca ccaggaattc ctaaaggagt ggaaataatt
     3721 ttagtgatag gattaataat aggttttacg gcaattccca aagcagcgat ggaagtgata
     3781 ataattaaat cctttacggt aaaatgctgg agcagtttca ttagataagt ttctttagtg
     3841 tattactttt taattcatat tgaatcagtt caattcctgc ctcgcgaaaa agctcttctc
     3901 ctaattcgtc aggataagtc tctgctatat aaacagtctt aatttcagcg ttgataatta
     3961 aacgagcaca aatactacag ggttgatttg tacaataaag aattgaaccg cgcgtggaag
     4021 aaccatttat agctgcttga attatagcat tttgttcagc atgcacacct ctacaaagct
     4081 catgattttt tccagaagga acattttgct gttttcttaa acaaccaatt tctgcacagt
     4141 gaggaacccc tttggggcta ccattataac cggttgaaat aatttgatta tcgcgaacca
     4201 aaactgcacc tacttttctg cggagacaag tgcttcgctt tgctgcaaga tatgccattt
     4261 ccataaaata ctgttgccag gaaggtcttt ccattttttc tcctgtagat tttgtatttt
     4321 tcaattatct tataacccta ctttctgtca atagctttgc tgtaacttaa aatcaacttt
     4381 ttacttaggg ggtcaggatt tgttactttc aataagaaaa aatgctccca agttatgaat
     4441 tgtaagaaag aaaaaagctg gttttatgtt acccaaaaga gaaagaacat acattgtatc
     4501 caggcagcag ttgtaaatga aattatatcc agtgtgtaaa ggtaaaagat ctggaaacct
     4561 gtttcgggag gttatcctaa ctactaaact gctaaaaaat atggatgatg caatgagcag
     4621 atatctttaa tacaagcttg atagtatatt ctttgtaggt tgatgtggac atcgccctcc
     4681 gccctccgcc ctcagccccc tgccctccgc tctccgccct cagccctccg ccctccgctc
     4741 tccgccctcc gctaaaatgc tcctaaactc cttgtttcta aatttacatc tcgccatttc
     4801 cctatttcgc taatcgctat tatcagaatt cccttgccaa aaaatccctt tccttatttt
     4861 aatggattca taataaaaaa ggggaagttc ataatggcag ataaattata tttaattgat
     4921 ggcaccgcac tattatacag ggctcatttt gcctttatca aaaatccttt gatcaacagt
     4981 aaaggaaaaa ataccagtgc tatctttgga gtaataaatt cttttttaca tatcatcgaa
     5041 aatactcagg cagcttatgt agccatttct tttgaccgta aagctcctac ttttcgtcat
     5101 actctctatg aagcatataa agctcagcgt cctccaatgc cagaagaact tactgcacaa
     5161 attgagccag tgaaaaaatt ctttgaatta attggtctaa aggaaatagg gatggatggt
     5221 tacgaagctg atgatatttt aggaactatg gcaaagcaat tcaaagatca atttcaaatt
     5281 gtgtttttaa ccagtgacaa agattactgt caattaattg atgagcgttc cgtgatgctt
     5341 gatccgatga aagatgtaat tttggatcgg gatgccgttt atgccaagta tggagtttat
     5401 cctgaacaat ttatagatta tttagctttg gtaggagacc cttccgataa tattcctgga
     5461 gtaaaaagta tcggacccaa aactgcctca atgttacttt ccgagtataa aacgctggat
     5521 aatatttatg cccatttgga ttttataaag ccgcgctgga aagagctgct tttagaaaac
     5581 aaagaaaatg cttatctttc aaaggagtta gctactatta aaactgatgt tcctattccc
     5641 aaaccggatt tagaatattt aactttttgg acgaaggacc taaagaaggc aattcctcta
     5701 ttggaagaat atgaaataaa ttccatcaag cgtaaaattg aattaaacta tccagagccg
     5761 acagatacaa tacaacaatc tgaatatcaa gatgatatct ttaatatgga aagcaaaaaa
     5821 tcagaagcgg tttcggaagc tgacacaaaa atgcctttta aagcaatttt agtgaatacc
     5881 cgcaattttg ttactttgtt aaaggaaata aaagaagcac agctgatttc gcttgatacg
     5941 gaaaccgatt cgccagatcc gatgctggca aatttagcag gaatatcctt ttgtatgaat
     6001 gaagcaaaag catactatct tcctttgggt caccagatgg cggataatct atcccttccg
     6061 gaagttatcc gtcatctcga agatgccata caaggaaaac tgctcttggg gcataacctc
     6121 aagtttgatt taattgttct ttgcagacat ggattgaact taaataatcc tatttttgat
     6181 actatgttgg ctgcttatat tcttgatccc ggcactttaa attattcatt agatgcctgt
     6241 gcggtaagcg aattgaatta taaaatgatc cccatcagtg atttaattgg taaaggcaaa
     6301 aatcaatcca catttgatta tgtagaattg gatagagcct gtatttattc agcagaagat
     6361 gcctgggtag tatataagct atatccgatt taccatagaa aagtagattt tagtgttatg
     6421 gcgaatcttt tttacacaat cgaattgcct ctaataaagg ttttacaacg aatggaaatg
     6481 aacggtatgg ccattgattg ttctgtttta acggagatat cccataaaat aaatcaggaa
     6541 ttgaagaaat taacagatat aatttatgct tatgcgggct atgaatttaa tctcaattct
     6601 acacagcaat tagcaaaaat gctctttgaa gaaaaaaagc tcccaacccg aaaaaaaacc
     6661 aaaacaggtt attctacgga taacagcgtg ttagaagaac tggcagaaga ttatgaaatt
     6721 gctggtacca taattcaata tcgacagctt gctaaactgc aatctactta tatcaacgct
     6781 ttacctaaaa tgataaatcc tgaaaccgga agaatacatt cttcattcaa tcaaacagtt
     6841 acttccacag gacgtctttc ttcttccaat cccaatctgc aaaatattcc tgtccgtaca
     6901 gaaattggac gcgaaatcag aaaggcattt tacgctaaag atgaagagca tttaattttg
     6961 gatgccgatt attctcagat agaattgcgt ttgttggctc tgatgagtga agatgaagtt
     7021 ttaatagaag cttttaaaca agacctcgat attcataaaa gaatggctgc caagatttat
     7081 aatgtatccc ttgaacaagt tactccagat caaagaagag cagccaagac tattaacttt
     7141 ggcattctct acggtatggg gcaaagaaaa ctttcccgcg aactgggtat ccctcaaaat
     7201 gaagcaaaac aaataattga cgactattat gcgcaatttc cttccatccg agagtttatt
     7261 aatcaatgta ttcttaaagc ccgccagcaa cattatgctg aaaccctttt tggtaggcgt
     7321 ttatatcttc ctaatataga aagtaaaaac cagggattga gaagtgaagc agaaagaatt
     7381 gctgtaaata tgcctattca gggaacagca gctgatttaa tcaaaatagc tatgctggat
     7441 attcataatc agatagataa acgggaagat ataaaaatga ttctacaagt tcacgatgaa
     7501 ttgctctttg aagttcataa aaaggcaata gaagaagcta aaataattgt tcaaacctgt
     7561 atggaacatg cgttacctaa agaatatgcg gataaagttc acctgaaaac agacctggga
     7621 gtgggagtaa attggtttga agcgcactga gaatgaagaa ttaagtcaga agctggcatc
     7681 gcttatataa ctttaaatca gccatataac caaattagtg gaagctgcat aaacattatc
     7741 tgtaggatac tatttctttt ctgcatcctc ggtatgtttt attctttcta accaatgtga
     7801 tttctttgga aagcgtaaaa tccaatgtac taggaaagtg agtagaataa gaacaattac
     7861 cccaataacg agtagcaacc aagggaagtc ctttcttaat tccacagaca taagttcttg
     7921 cttatgccac attttacggg tatcatattt ccaggcaacg gtacgattat tataatctac
     7981 attttccggt aaggcattgg tagaaataat tttccaaggt agatgaactt tatatgtcca
     8041 tacaggatta tcgggttgta atctaccaat aatatgttca atatcatctt cgtcttcagg
     8101 ttcctgactg ccaagagcaa ttcttctttt aaaagttatt cttcttccgg gttctttatt
     8161 cagagttatt tttccccaaa aatcagctgt tcctaattga tcattcacat tattaaaagc
     8221 atctatgttt ttgaatttaa agttaatagt ataaataaca tcaggacctt ttcggtaaat
     8281 gtcataggat tgtaaattta ttcctggtaa agctgctttg cgcataattt cttctgggtt
     8341 ttcataagga gaacgatgaa ctatacggat aacagctctt cctgagccat tgcgattaat
     8401 ccatagttct tcatcatatt gaacgcatcc agccaaaacc agaagcgcta gcagcagcat
     8461 tataaggcgt ttcatttagc aatccttctt tattaataaa gctttataaa tcaataaaac
     8521 aaaaagcgtt ttttttggca agggaaaaat ttcagttgac aaaagtacta aattgcttat
     8581 aataaaagaa agagaatcaa ttagggagga aagaaaatga tagcattaag acaactatgg
     8641 cgttgcgcta tttgtggaaa tgtagttgaa gtaacttatg ttggaggtgg tgaattagtt
     8701 tgctgcggtc aaccaatgta cttgttacag gaaaacacag tagatgctgc aacagaaaaa
     8761 catttacctg tcgtgaaaga tctgggagac aaaattgaag tctctgtagg aagcgttcct
     8821 catccgatgg aagagaaaca ttatattgta tttattgagg ttctaaccga taagaaagtt
     8881 ttgcgcaaag aactgaaacc tggagaagaa cctaaagcta tttttaaagt atcgatggat
     8941 aaggttaaag aagtgcgtga atattgtaat atacacggac tttggaaagc ataactataa
     9001 taaggagaag aaaaatgtca tttagagaaa caagaactgc tgaaaatctg atgaaatctt
     9061 tcgcaggtga aagccaagcc agaatgagat atttatattc agctaaaaca gctaagaaag
     9121 aaggctttga gcaaatatct aatattttta cagaaactgc agataacgaa aaagaacatg
     9181 ccaaggtatt cttcaaacat ctattgagta atggtgtgga aggtgaaatg attaatatca
     9241 ttgcttctta tcctgttggt tggtctgcag ataacacact aaaaaatctg gaatatgcag
     9301 caaatggtga aaatgaagaa tggtcggaat tatatccttt gtttgccgat gttgcagaag
     9361 aagaaggttt tagcgatata gctcattcct ggagaatgat agctaaagtg gaaagagaac
     9421 acgaaaagcg ttttcgcaag ctctatgaca atgtgaaaaa ccagaaggtc tttattcgcc
     9481 aggaaaaagt cctatggaaa tgtttgaatt gtggatatgt gcatgaagga actgaagctc
     9541 ctaagatttg cccttcctgc agtcatcctc aaagttattt tgaactcttt aaggaaaact
     9601 actaaaaact gcaaggagac caaaaatgca aaaatatcaa tgtatggcat gtggatggat
     9661 ttacgatccc gctgaaaaca acaacattcc cttcgaagag cttcctgatg attttgtctg
     9721 cccagaatgt ggtgtcggaa aagatatttt cgaaccctac gaataactaa ataacctttc
     9781 tggccactgg ggagttttat ctctccagtg gcttaacaaa aaataaatga tatgtgaggt
     9841 ctaaagtgca acaacaggaa tttgaagaaa ttttggattt tgctatctcg cgtgaagagg
     9901 aagcggtaaa attctatcaa gaattgcaag aacaatccca atttcaggat gtaaagaata
     9961 tgctgaagga tttggaaatg atggaaaatg gtcatattca gataattgaa aacattcgta
    10021 taacaggtgt gaaagaagaa gatattcaga aagtgcccaa tttgaaaata agtgaatact
    10081 tatgcgtaga tccctctgat ctggatatga gctaccaaaa tttacttgtt cgagctatga
    10141 aacgagaaga aaactccttt ctgctctatt cggaaatgag cgtgaaattt cccgatgcag
    10201 aaatagctac tctttttaga cgcctggctg ccgatgaggc aaagcataaa ctgttttttg
    10261 aaaacttata cgacgattgg atcaggactg gaaattgaaa cttgccatag attctactca
    10321 aaattccggt tctatagctt tagcaaatga agagcgggta atctattctg cctattttga
    10381 tataaagata acccacagtg aaacattgat gccagcaatt gactacgcat tcaaattttg
    10441 taatattttg cgtagtgaat tacaagaaat ctatgtgtgc ataggtcctg gttcatttac
    10501 cggactaaga atcggattgg caaccgcaaa agggattgcc ttcgctttag gaattcccct
    10561 atatgcatat tcctctttgg aattggcagc attaccagca agcggattgg gaaaaaatat
    10621 tttcgctgcc gttgatgcta aaatgcaaga agtttattat gcctattacg atcagcagct
    10681 aaaagaaatt atacctccct ccattgccac gcctgaagaa ttatgtaatc tggatttgga
    10741 ggattttatc ttatgcggaa gcgcttcaga aatgcttgct ctgcggttta aaaattccta
    10801 taatttttat actctccatc ctgttatgaa aattccttcg gcagcagggc tgttttctat
    10861 accaaacttg ttaccggaaa agcacatccc ccaaattttg gaagaattgg aacccttata
    10921 tttaagagag gcaagggcgc aggtaaaaaa agcaaaacca aaacagtgaa aaaaataaga
    10981 aaaaatgcac ttgttacaca acaatctcct gtttactcgg ttgctgcata cataatttaa
    11041 cgatagcagc attcttttat gggaaccagg attgtttctt ccttagttat ttgtggtaga
    11101 gttgcggtgg agctacggcg ggaaaatgcc gagtaacacc attgtagctc agcggtagtt
    11161 aataagtagg aaccaagcaa aaatgaataa gttagtttca attgaggaag taaacatagg
    11221 atcaaattat ttttcagata accctcggga aataacttga cggattggtt gattttatga
    11281 taaaggaaca gaacttgctg taattatctt agcagaacaa aaaaaaagat ggatatgaat
    11341 atgaaaaaag cttttatatt actaacggcc atcctgattg gaatcaattt ggctgccttc
    11401 gaaacagtga ttaaagtaga taagaatgcc tggcaaacga aacaacaggc agatggttat
    11461 cctgttacat tacaaactgg tgagccggtt cttccttatg taccggtaaa tgtgctaata
    11521 ccttttgggc aaagatatca aaatgccagt attcaattag ctccagctga acttgtggca
    11581 gaaagagtaa cttttgaaat tgctggagtt caattgccta tttccagaat gaatattccc
    11641 aatgccggtt cttattcgtc agcggttttg gagaataagc tttatcctga aagggagtgg
    11701 cagtttttag gaattcaata ttttcgtggt tatcaaattg cgcttttcaa tgtttatcct
    11761 tatcgctata atcctattac gcagaagctg tatgttagtt ctgagattag tgtatccatc
    11821 aacagtgaat tttcggaagc agaagcagaa tatcaagcga atttttacac tcctaattct
    11881 aaaactctat cttatataaa ttcgctgatt tgcaatcccg aaaatatagc aagctatcaa
    11941 tctgcaagta attatagaag tgtacccatt ttaagccggg atattgatct tacggttccc
    12001 aaacaaatga ttattattac tagtaattcc cgaatcaatt ggtttactga atattcttct
    12061 tggcgtgcca gtaaaggtgt ttccaatgct atttatgctc tggaagatat tcttagttcc
    12121 tatcccggaa ccgataatgc agaaaagctt cgtaatttta ttataaatgt ttatcagacc
    12181 tgggcaaata gttctcaacc cttggaatat gttattctgg gcggtgatga tgaaattatt
    12241 ccagagcgag gcacttatgg ccaagttggg gatactatag acgaaagaat gcctacggat
    12301 atttatttta gtaatttaga cggtaactgg aatgctaaca ataataatat ttggggtgaa
    12361 ccagctgata atccagatat gattccagag gttcatattg gtagatttcc tgctgaaact
    12421 caaaatgaat ttaaccatat gtttaataaa atccgctatt atgttgataa cgatacttat
    12481 agcaataatt atgcgttatt tatgggcgaa aacctgaata acaatccttt aacttgggga
    12541 ggtgattata aagatgatgt ggcacaatac cttcctgatg aatattattt gcaaactcta
    12601 taccaaaggg atggcactta tagctctatg aatgtttata atgcgattaa caaaggcgct
    12661 ggaattatga atcatatggg gcatgcaaat gaatcagttt taattggtca atccaatgga
    12721 accgtagaaa gtttaactaa tacagaatac ggttttctat atactcaggg ttgttatcct
    12781 gctgcttttg atcaacgtac ttctggcgat aatgaatcaa tcggagaaca ttttgttacg
    12841 gcttctggag gagtattttg ttttattggc aatactcgtt atggatggta tagtcctgga
    12901 agtgttaatg gtgcttctca attttatgat cgtcaatttt ttaatggtct atttcaacag
    12961 aatcttcctg ttctgggaga ggctttaacc tattcccgtc tgcaaaattt gaatgcagca
    13021 ttaagtaatg atgtaatgcg ttggtgttat tacgaagtag ttctttttgg tgatccttcc
    13081 atagcagtaa aatcttttaa tcctaatttg cctttgctta gtttggagag ttatgaaatt
    13141 accgatgaag aaggtgataa taacggaaca atcagcactg gcgaactaat ttgtatttat
    13201 cctgttatct ctaataacac gaattggggt acagcatata atgtatcagt gagattagaa
    13261 aatttacctg aaggcattga agtggtaggt aacaatttat ctattgctca aattcttcct
    13321 ggtgggatga gccctcaaaa tttgaaaatt cgtttgcaac tatctgatac tgttggtttt
    13381 ggcacttact accttaccct ggttattgat tctataaatc ctgtaactaa tttatcaact
    13441 ggagaacgga gatttactgt tagttttgag attacattaa tggatgatcg ttttccttgg
    13501 gaaactatta taaatggaaa atcagcacct ttggtaacaa atttgaattc taccagtgga
    13561 atggaaatta tctatgttga tgtgtatggg aatagttatt accttggtaa cgatggtgaa
    13621 atttattcta cagtagaagc acctgaaggt atgtatatat tccgcagctt tgcttgtgga
    13681 cctatagatg atgatgatga aaatgatctg gttttttgta gccgtagcgg tgatatttat
    13741 gctatgcatt ttgatggtga gataatcttc aattaccatg ataatacaat gtttttattc
    13801 actcctgtac tggcagatat aaatggagat ggaactttag aaacaattgc cggaagctta
    13861 tttggaaaaa tctatgccat ttcccccaat ggacaattgc ttaatggctt cccgtttgat
    13921 gttggcggca atttccaaac tgaacttgct gctgccgatt ttaatggtga tggaactatg
    13981 gaaattgttg ctggttccca aaatggactt ttatatgttt taggaacagg tggaacagtt
    14041 cttcccggtt ttcctgttca gctaaatggt cctgtaactg gttctcctac gattacaaat
    14101 acaaatagaa tagtatgttc cactcctacc catatctata ttatttctgc ggaaggaaat
    14161 atcatcgcga tgcgtaatat tgatgctaat atagccggtg gttttgcttt gggagatatt
    14221 acttctgatg ataatggatt ggatattgtt ggaataacaa acaacgggat tatttatgcc
    14281 ttaacagatg aaggtgttga tcttcctggt tatcctgtag caactggaga taattttgtt
    14341 tgccctcctc ttattgctaa tttggatagt gatcctcaag ccgaaatttt agtacatagt
    14401 tatgtaaatt ctgtgtatgc gtttaattct gatggcagca ctct
//
LOCUS       DAORHB010000011        14415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3259_length_14415_cov_4.869290, whole genome shotgun sequence.
ACCESSION   DAORHB010000011 DAORHB010000000
VERSION     DAORHB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14415
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3259_length_14415_cov_4.869290"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..323
                     /locus_tag="PKM80_00740"
     CDS             195..323
                     /locus_tag="PKM80_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92463.1"
                     /translation="MSFFQKSIAGGRRSSVRKKSAELQRAPATHLGTGTLALGFLA"
     gene            741..1901
                     /locus_tag="PKM80_00745"
     CDS             741..1901
                     /locus_tag="PKM80_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92464.1"
                     /translation="MKKIILLAFILGVVIANLAAISNYVLMVSCFEPSAINLALGGSP
                     VGVVNIWHNEPLNAYDNPAYPSLHTGLSYSNTCYDFMRLKHPGDEKESAYYASMMSLS
                     YKGFGFLFPSVTNVHTWGINADYGSIAFVDENGYEIGITKQLKEFMYPLGISVNFADL
                     YRIIHNDNTFSNKKLDLCLGMNYILNCSSIDIGYSYPANIGEEYDADCVDSGALLKYS
                     FPLTDKIHLESVAGFSMFNAYGDGIKYNSGQQHKIYKRINTGIACSASLKNPNYLKEN
                     ELTDNFENIGCLRLLAGMNDDRTQSPNIYGIGTELGFLDTIFLRSGYHKDKGGNIEGL
                     TYGIGLDLHYRNLVSLNGNYSYFPGGSQCKDKNIYSFGCNLNVFQIASEMLK"
     gene            1922..2302
                     /locus_tag="PKM80_00750"
     CDS             1922..2302
                     /locus_tag="PKM80_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV92465.1"
                     /translation="MNMTKIYILAECIFLLIMLMGCSLATKPESTADESTANDNCLME
                     VYPNPVVKGETVVVNFNFHLAGLYKLVITNVLEQEVISFDITDNSLIWDGRDKYGQLC
                     ETGVYYITLYSDELVLSRKILLFA"
     gene            complement(2488..2610)
                     /locus_tag="PKM80_00755"
     CDS             complement(2488..2610)
                     /locus_tag="PKM80_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92466.1"
                     /translation="MNNLSNLHNLHNLRNLRELFPVALVAKYSSNILAYKVNIS"
     gene            3260..4810
                     /locus_tag="PKM80_00760"
     CDS             3260..4810
                     /locus_tag="PKM80_00760"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNV92467.1"
                     /translation="MENQNPLEKLRAKRAAALLGGGEKRIVEQHLKGKLTARERIALL
                     VDPDSFEEFDMFVTHRCTNFGMERYKHPGDGVVTGCATINERLVYIFAQDFTVFGGSL
                     SKTYADKICKVMDMALKNGCPIIGLNDSGGARIQEGVDALAGYAEIFWRNVMASGVIP
                     QISAILGPCAGGAVYSPAITDFVVMEKSNSYMFVTGPKVVKTVLNETVTNEDLGGAKV
                     HTSKSGVAHFIANSEEDAMLTIKLLLSYLPSNNLEDPPYVPNADPITRNCDVLNNLVP
                     ENPNKPYDMLEVIYPIVDNGEFLQVMSNFAQNIIVGFGRMNGRTVGIVANQPKILAGV
                     LDIDASIKGARFVRFCDSFNIPLVVLEDVPGFLPGTMQEHNGIIRNGAKLLYAFAEAT
                     VPKVTVIIRKAYGGAYCVMNSRHMRADIVYAWPSAEIAVMGPEGAVEVIFRKEAMASE
                     DPKAILQAREKEYREKFANPFVAAERGYIDDIIEPSATRFRLIRALEMLANKQDSIPP
                     RKHGNIPL"
     gene            4821..5666
                     /locus_tag="PKM80_00765"
     CDS             4821..5666
                     /locus_tag="PKM80_00765"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNV92468.1"
                     /translation="MRKIVFLAIFACSFLSAFAQIDLIEQTISDSLALVREKEILSEY
                     GFRDTATLAEVAEKLQIKDIASWKKYLGLEPYNEKLDKMSLRKLGITPYKALLAKQYS
                     VYGFTELSTLDEVASVLNMPIKKIKQKIGLEKPNSQQWDSYSLQSLNVTPEDIIKIEQ
                     EFNEQSMNYGLNVTLVGMLTVFTALLITSIIVGQLNLLNRPPKKEKADLKISPGGKVR
                     AASATLNQNVIAALVTAVMLHEQTLEDQRKIALTFKRTPTNQWRANAMLNMPNREMNN
                     RRRYR"
     gene            5663..6376
                     /locus_tag="PKM80_00770"
     CDS             5663..6376
                     /locus_tag="PKM80_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNV92469.1"
                     /translation="MKTYKLLINNEHFEAKILEYSPTHARINLNGTEYLVQIENDKID
                     SVPKLAAQEKAVPLAPAFSSNADLFSGEIRAPLPGVIVTIPVSEGDEVKAGQSILIIE
                     AMKMQSEIASPFDGKIEKILVKERAPVKEGDLLMKITGVDIKSPPISTSPKHSPEKES
                     EKTGNKDKILRAPLPGTIMDIKVQVNDYVKEGDIVLILEAMKMESDIYSPRTGKVSKV
                     YVHKGDLVQDNDPLIEFED"
     gene            6381..7508
                     /locus_tag="PKM80_00775"
     CDS             6381..7508
                     /locus_tag="PKM80_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNV92470.1"
                     /translation="MHELSQFIQTTGFLNVTWGNLVMIISGMIFIYLGVAKEFEPLLL
                     IPIGFGIIVGNMPGVSQQGLGVMNEGSVLNYLYFGVNKSIYPSLIFLGIGAMTDFSTL
                     IANPRLILLGAAAQFGIFATFIGSLLLGFNVMEAASIGIIGGADGPTSIFLASKLAPH
                     LLGSIALAAYSYMALVPVIQPPIMKLLTTKKERLIRMKPLRVVSKKEKIIFPIVAFVV
                     TTFISPGSVVLLAMLFLGNLLKESGVTERLANSARTVLIDVVTVLIGLTVGAKTTAEV
                     FLTPATLKIFFLGAFSFAAATAAGVLFAKIMNLFSKNKINPLIGSAGVSAVPASARVS
                     QVVGQQYDKTNYLLMHAMAPNVAGVVGSAVAAGVLLGILGK"
     gene            7546..8466
                     /locus_tag="PKM80_00780"
     CDS             7546..8466
                     /locus_tag="PKM80_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HNV92471.1"
                     /translation="MPKVLIATEKPFAADAVQKIKTELENAGYNYSFLENYTEKSALH
                     NALADAEALIVRSDIIDEAAFNAAPKLKIVVRAGAGYDNIDLKSATAHNVVVMNTPGQ
                     NSNAVAELAIGMMIYMARNKFNGKSGTELAGKTLTLYGFGWVARYVAKIAKGIGMDVY
                     AYDPFISDEVIQKEGVKPLHSVEDIFKLGDYISLHIPATEDTKKSVNWNLLSLVKDDA
                     ILVNTARKEVIEEDALLKAFAEKKKFRYVSDVAPDNSAKLLELYPDRVFFTPKKMGAQ
                     TSEANTNAGIAAAKQIIAYFEKGDVKYKVN"
     gene            complement(8732..10123)
                     /locus_tag="PKM80_00785"
     CDS             complement(8732..10123)
                     /locus_tag="PKM80_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV92472.1"
                     /translation="MKSNDLTHILVLDDETSIRDGLIHYFSERNYRISGASNWEEAQL
                     VLTKSIVHIIILDVVLPGFDRKKDGLDVLSLIKQKYPDTEVIMISGHATVEKVITAWN
                     QGALMFLEKPYSLEEMEKAVKRAEYYSRGFSRLHQLEAQVISQKMEQSLGTKVIAKSP
                     QMKKCLSLAKKYAQNNHSVLITGESGTGKEIIAKLIHYFSNRSEEEFVAVNVNAIPHE
                     LFESTLFGYVKGAFTGANTNTTGLVEKAGKGTIFLDEIGDLPMEQQVKLLRLIETREY
                     YKVGSSRPSRTEARFIFATMQNLNQKVEQQSFRNDLFYRISDLEINIPPLRERKEDIS
                     ALCEYIIPQLAKEEGIAKIPVLTKDAYKAILTYSFPGNIRELKKILRKAILNLESNEI
                     LKEDLFPPVEQNVTKTKGTFCPDCEAYFIKKVLTNTDFNASLTMKLLGISRSSFYDKL
                     KRYNINVKHNIEE"
     gene            complement(10110..12227)
                     /locus_tag="PKM80_00790"
     CDS             complement(10110..12227)
                     /locus_tag="PKM80_00790"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV92473.1"
                     /translation="MEEKEIKLLEKALSGKPTKDNFYQGLKLVSYYIGCNPHKAEEST
                     QILLQMANLMGTAEEIAFANKTMGSVNSVLGNHQEAIPYYQKAVNLFSQIGKNLQMAQ
                     SLASIASMNTTLGNTAQALKTFMEVIPLFEQENDQHSIAITYSNMANIYKAKMDYAQA
                     IQYYQKAADLAESIQCLDLQVSSLCIMGSIYSSLMDYNKNLACCLKALTLAESTGNNY
                     LIGEVHCSLGVAYQELQDYDKALEYYYKSLKIGREQQIPYYISGNLSNIADIYRKQGK
                     LKEALKAYKEALKYDLQTGDIYNQSASLRTIALTYIDMHRYALARKTILQALNIISAD
                     EHLEMGINLQADLLLTCIKQQDYKYAEALLKKLLKKVQDNPVSEVQISIYEYAAGLYT
                     KLRNYKKANYFLNLHSQLYKEFINQEREKINRFMQISFETARLQDEKEMLQFQNELLE
                     KRVQAELNKIKEKDKLLAQQEQLAMLGKISAGIAHEINNPLAAIKQIVEITLEDLNSL
                     QPRGYLSQKKNDVLQLLERINRLVEIIKVIARSPERFNANSFSLNQIIEEFIELFGDK
                     IISKDVLLIKDCEPDLPPVYADNIRFIQLLSILATNSNDALKNVNRSEGKYITISTKF
                     IDGRIMLVVEDNGCGMDQETLSRATEPFFSSKEIGQGTGLGLSLAHSIVLNSNGNMTM
                     ESELGKGTSVSLSFPVEDHYEKQ"
     gene            12568..12690
                     /locus_tag="PKM80_00795"
     CDS             12568..12690
                     /locus_tag="PKM80_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92474.1"
                     /translation="MTKLPVDFTVVNVGINGILMLGIAVISPICLESSNIFISY"
     gene            complement(12720..13814)
                     /locus_tag="PKM80_00800"
     CDS             complement(12720..13814)
                     /locus_tag="PKM80_00800"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNV92475.1"
                     /translation="MFYCPTRIIFADDAIQKAKDKIAVLGKKALLVSGEKSAKQSGAL
                     SDLITVLNELEIHYVLFDRIKENPTLDSVMEGKQIFLQNNCDFVIGIGGGSPIDAAKA
                     ISLAAANSLNRDEIYNIAAFKNVYPIMAIPLTAGTGSEATQYSVLSDPTHKKKAGLGS
                     DLAFPALAAIDPKYTLTLTPKVTMHTALDALSHLLEGLYSNQRSPLVYPFIYKGIASI
                     MKCLPRALSEPDNLAARKELMQFALYGGIVIAQGSTTLQHSIGYPLTSNYNIPHGLAN
                     AMVMEDIMELYYPAVSKELNDLFAYLNLSKDAFYAWLRALPFERKIELSDTFIEEAIP
                     QVMGSRNMALNPLPVSEEHIRIIFEHIRKS"
     gene            complement(13823..>14415)
                     /locus_tag="PKM80_00805"
     CDS             complement(13823..>14415)
                     /locus_tag="PKM80_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV92476.1"
                     /translation="LISAHSDIYHNLTVDPDKTIVSWNSMLPYIYTFNNAAEPIYTAN
                     MIDGSNGAGQCIAFKHDFNGTFVMFGFPLYFMQYPEVRIMLHQLLPTLNPALPITDET
                     VAMPEVSISVMPNPFNPSTTISFYLPVSGYIECELYNLKGQKVTTLAKEKMTAGKHSI
                     SFNGCDSQGRYLASGVYFLRLQHPNGVINKKITLLK"
BASE COUNT         4449 a         2528 c         2848 g         4590 t
ORIGIN      
        1 gacgggtttt aacccggcgc aataaagaat gggtataaaa gtcccagcgg gacgatacat
       61 attaacgacg ggttttaacc cgttgaaaat acaggaaggt tgacctcctg gtcaacccac
      121 aacaaacaaa ctaccaagct atcctaaaat atagtggaaa caatatagct tagttgtcag
      181 gaggaacaac gaggatgtca tttttccaga aaagtatagc cggaggacgc cgttcctccg
      241 taagaaaaaa aagtgcggag cttcagcgag ctccggctac ccatttagga acagggacat
      301 tagctttagg gtttttggct tagtggagaa ataggccaaa attatgtcat atttatctgt
      361 attttacctt tttgccttct caaaccccgc aaatttattc attactttcc ctttttgcct
      421 caccaaaccc cgcaaattta ttcattttct gcctattttc catactctgg gcacttctca
      481 cactctaaaa tcccagctta tatgccaaaa gaagatatct tatactaaca tagaacattc
      541 cgtaacgact ctcttacact tctctaacgc tgccgtagtt tcgttagaga agtgttacgg
      601 aattgtttct ggactataag ttaagagagc aggcaaggca tttcagaaaa aagtgttact
      661 attggtaggt cggaataaaa ttcttgacag aaaaagataa tattttacaa gtaaaaaaag
      721 aaggttttga ggggtttttt ttgaaaaaaa taatcttgct ggcttttata ttaggtgtgg
      781 tgatagcaaa tttagcggct atctccaatt atgttttaat ggtaagttgt tttgagccct
      841 cggcaattaa tcttgccttg ggtggaagtc ctgtaggagt tgtaaatatt tggcacaatg
      901 aacctttaaa tgcttatgac aatcctgctt atccatcttt gcatacaggg ctcagttatt
      961 ctaatacttg ctatgatttt atgagattga aacatcccgg agatgaaaaa gaaagcgcct
     1021 attatgcttc aatgatgagt ttatcttata agggttttgg ctttcttttt ccttctgtta
     1081 ccaatgttca tacttgggga ataaatgctg attatggaag catagcattt gtcgatgaaa
     1141 atggttatga gataggaatt acgaagcaac ttaaggaatt tatgtatccc ttgggaattt
     1201 cagttaattt tgccgatctc tatcggatta ttcataacga taatacattc tcgaacaaga
     1261 aattagacct ctgcctcggt atgaattata ttctaaattg cagttctatt gatattggct
     1321 atagttatcc cgctaatata ggtgaagaat atgatgctga ttgtgtagat tccggagctc
     1381 tgttaaaata tagttttccc ttaaccgaca aaatacatct ggaatctgta gccggatttt
     1441 ctatgttcaa tgcctatggg gacggtatta aatataatag tggtcagcaa cataaaatat
     1501 acaaacgcat aaacactgga attgcctgtt ctgcctcgct taaaaatccg aactatctga
     1561 aagagaatga gttaacggat aattttgaaa atattggatg cctgcgtctt cttgccggaa
     1621 tgaatgatga tcgaacacaa agccccaata tttatggaat tggaacagag cttggttttc
     1681 tggataccat ttttttaaga agtggctatc ataaagataa agggggaaat attgaaggtc
     1741 ttacttatgg cattggtttg gatttgcatt atcgaaatct ggtttcactt aatggaaact
     1801 attcttattt ccccggagga agccaatgca aagataagaa catatacagt tttggttgca
     1861 atttaaatgt ttttcagatt gcttcagaaa tgcttaaata aattataggt gaggaactaa
     1921 gatgaatatg actaaaatat atattcttgc cgagtgcatt tttctgctta taatgcttat
     1981 gggatgcagt ttggctacaa aacccgaatc cactgctgat gaatctactg ccaatgataa
     2041 ttgcttaatg gaagtttatc caaatcctgt agtgaaaggg gaaactgttg ttgtgaactt
     2101 taactttcac cttgcaggat tgtataaact ggtaattacc aatgtgttgg agcaggaagt
     2161 catcagtttt gatattactg ataattctct gatctgggat ggaagagata aatatggaca
     2221 gctttgtgaa accggggtct attatataac cttgtattca gatgagttag ttcttagcag
     2281 gaagatatta ttgtttgcat agatgcttgt ctgaacgggc aaaaatgaaa tataccaagg
     2341 atgaacggga ttatatttgc agagtgtaga gagcttaggg ctaagagaaa aggtctgcga
     2401 ttttaggatt agtacccaaa gatttagtct tagaaacaag gatttaaggg tttaaatgtt
     2461 agaaacaagg atttaaggat tataggatta agagatatta actttataag caagaatatt
     2521 agaagagtat ttagccacca aagccaccgg aaataactca cgcaagttac gcagattatg
     2581 cagattatgc agattactca ggttattcat atatgagcag cttaaggttc aaaggcatcg
     2641 ctgtagcgaa ctcgcatcct ttttgtagaa tttttattca tttctttctc cttaatttta
     2701 gtccactgat ttcaggatgg ctcaagcaaa caacccatca tcctctcctc ctcttctcct
     2761 cttcaactca tcgttaagga tgagataaga ttaggtggag gacaagttga atatcggtaa
     2821 cttgcaagaa atgtaaggtt taggttgaaa cgatctataa atgttgttaa tattcctggt
     2881 gttagtgaat ctcaagagct tcggattatc gattataata tacttaatgc atagataaac
     2941 aatctgctat atattggaag tgttagtatg caagaagttg gcaaaaagaa aaaacggtca
     3001 agccaattgg atcggtgtac acattatgca atgtaatctg catttgcagt acaattcatt
     3061 ttgaaagata tgtgtagcaa tcattgctat aataatatga atattttggt tttgccagga
     3121 aaacaataga agacagcttt agatatcaca actataaaaa aacccagaac atttcccttt
     3181 actaaaaaac agcttttaaa ataatgacta tggcataact taatacaaaa aaataaacat
     3241 agaataggaa aggaaagaaa tggaaaacca gaatcctctg gaaaagctaa gagctaaaag
     3301 agctgcagct cttttgggtg gtggagaaaa acgcattgtt gaacaacatc taaaaggaaa
     3361 gcttacagcg agggaaagga ttgccctctt ggttgatcct gacagttttg aagaatttga
     3421 tatgttcgta actcatcgtt gcaccaattt tgggatggaa cgatataaac atcctggaga
     3481 tggtgtagta actggttgcg ctacaatcaa tgagagattg gtctatattt ttgcacaaga
     3541 tttcactgtt tttggtggat ccctctctaa aacttacgca gataaaatct gtaaagtaat
     3601 ggatatggca ttgaagaatg gttgtccgat tataggttta aatgattctg gtggagcaag
     3661 aattcaagaa ggcgttgatg ctcttgccgg ttatgctgaa atattttggc ggaatgtgat
     3721 ggcaagtgga gtaattcctc aaatttcggc aattttaggt ccctgtgcag gtggtgcagt
     3781 ttattctcct gctatcacag attttgttgt gatggaaaaa agcaacagtt atatgtttgt
     3841 aactgggcca aaggtagtta aaactgtttt aaatgaaacg gttactaatg aagatttagg
     3901 cggagccaaa gtgcatacca gtaaaagtgg tgtagctcat tttattgcca atagcgaaga
     3961 agatgctatg cttactatca agttgctgct aagttatttg ccttcaaata atttggaaga
     4021 tcctccctat gttcccaatg ctgatcctat cactcgtaat tgtgatgttt taaataatct
     4081 cgtacctgaa aatccaaata agccatatga tatgctggaa gtgatatatc cgattgtgga
     4141 taatggtgaa tttttgcaag tgatgagtaa ttttgctcaa aatatcattg ttggttttgg
     4201 aagaatgaat ggacgcacag taggtattgt tgcaaatcag ccaaagattt tagctggtgt
     4261 tttagatatt gatgccagta tcaaaggagc cagatttgtg cgtttttgtg attctttcaa
     4321 tattcctctg gtggttttgg aagatgttcc tggtttttta cctggaacga tgcaagagca
     4381 taatggaatt attcgtaatg gggcaaaact tctttatgcc tttgcggaag ctacagttcc
     4441 caaagttaca gttattatcc gtaaagctta cggaggtgca tattgtgtaa tgaatagtcg
     4501 tcatatgcgt gcagatattg tttatgcctg gccatctgca gaaattgcag ttatgggtcc
     4561 ggagggagct gtagaagtaa ttttccgtaa agaagcaatg gcttcagagg atcctaaagc
     4621 cattcttcag gcacgagaaa aagaatatcg ggaaaaattt gccaatccct ttgtggctgc
     4681 agaaagggga tatattgatg atataattga accctctgcg acaagatttc gtttaattcg
     4741 cgctttggaa atgctggcta ataaacagga tagcattcct cctcgcaaac atggcaatat
     4801 ccctctgtaa ggtataggca atgcgaaaga tagtgttttt agccattttt gcctgtagct
     4861 ttttgagtgc atttgctcaa atagatctta tagagcaaac aatttctgat agcttagctc
     4921 ttgtccgcga aaaagaaatt ttgtcggaat atggctttcg cgatactgct acccttgcgg
     4981 aagtagcaga aaaattacaa attaaagata tcgccagttg gaaaaaatat ctgggtttgg
     5041 aaccttataa cgaaaaattg gataagatga gtttacgcaa attgggtatc actccttata
     5101 aagctctctt agcaaaacaa tatagtgttt atggctttac agaactttca actctggacg
     5161 aagttgcctc agttctgaat atgcccataa aaaaaattaa gcaaaagatt ggattggaaa
     5221 aacccaattc tcagcaatgg gattcatact cattacaatc attgaatgta actccagaag
     5281 acattataaa aattgaacag gaattcaatg aacagagtat gaattatggg cttaatgtaa
     5341 ctctggtagg gatgctgaca gtttttacag ctttattaat taccagtatt atagtgggac
     5401 agcttaacct tttgaatcgt cctcccaaaa aggaaaaagc cgatcttaaa atatcacccg
     5461 ggggaaaagt tcgtgctgct tctgccactt taaatcagaa tgtaattgct gctttagtta
     5521 cagctgtgat gttacatgaa caaactctgg aagatcaaag aaaaattgcc ctaaccttca
     5581 aacgcactcc tacaaatcaa tggcgtgcaa atgctatgtt gaatatgcca aacagagaaa
     5641 tgaataatag acggagatat agatgaagac ctataaacta ctaatcaata acgaacactt
     5701 cgaagccaaa atcttggaat atagtcctac gcatgccagg ataaacttaa acggtacaga
     5761 atatctggtt caaattgaaa atgataaaat tgatagtgta ccaaagcttg ctgcgcagga
     5821 aaaagctgtt ccattagctc ctgcatttag tagtaatgca gatctttttt ctggagaaat
     5881 tcgcgctccc cttcctgggg taatagttac tatccctgtt tccgaaggtg atgaagtaaa
     5941 agcaggacaa tcgatcttaa taattgaagc gatgaaaatg caaagcgaaa ttgcctctcc
     6001 ctttgatgga aaaatcgaga agatactagt gaaagaaaga gctcctgtta aagaaggaga
     6061 tttgctgatg aaaattactg gggtagacat aaaatctcct cctataagta cttctcctaa
     6121 acacagtcct gaaaaagaaa gcgagaaaac tggaaataaa gataaaattc tacgcgcacc
     6181 tcttcctgga acgattatgg atataaaagt gcaggttaat gactatgtga aagagggaga
     6241 tattgtttta attctggaag caatgaaaat ggaatcggat atttacagtc ctcgaaccgg
     6301 aaaagtaagt aaagtttatg ttcataaggg agacctggtt caggataatg atcctttaat
     6361 tgagtttgag gattagcttt atgcacgaat taagccaatt tatacaaacc acaggattcc
     6421 tgaatgttac ctggggaaat ctggtgatga ttatctccgg aatgatcttt atctatttag
     6481 gtgttgcaaa agaatttgag ccactgctac tgattcctat cggttttgga attatagttg
     6541 gtaatatgcc tggtgtttct caacagggat tgggagttat gaatgagggc tctgttttaa
     6601 actatcttta ttttggagtg aataaaagta tctatccttc gcttatcttt ttaggaatag
     6661 gagcgatgac tgatttttcc actctaattg ccaatccgcg gcttatcctt ttaggagctg
     6721 ctgctcaatt tggaattttt gctaccttta ttggcagtct ccttttagga tttaatgtga
     6781 tggaagcagc ttcaatagga attattggag gcgccgatgg tccaacctca atttttttgg
     6841 cttctaagtt ggcacctcat cttttgggtt caatagcttt agcagcatat tcatatatgg
     6901 cattggtgcc tgttattcaa ccacctatta tgaaattgct aaccactaaa aaagagcgtc
     6961 ttattcggat gaaaccctta cgagtagttt ctaagaaaga aaagatcatc tttcctatcg
     7021 ttgctttcgt agttacaact tttatttctc caggcagtgt tgttctttta gcaatgctct
     7081 ttttgggaaa tttgttaaaa gaaagcgggg taactgaacg ccttgccaat agtgccagga
     7141 ctgttttaat cgatgtcgta accgttttaa ttggtttaac agtaggagca aaaactactg
     7201 cagaagtatt tcttactcca gccactttga aaatcttttt cctgggtgca ttttcttttg
     7261 cagcggctac cgctgcaggt gttctttttg ccaaaattat gaatctcttc tccaaaaata
     7321 agattaatcc tttaattggt agtgcgggtg tttctgcagt tccagcttca gcacgagttt
     7381 cacaagttgt tggtcaacaa tatgataaaa ccaattattt attaatgcat gcaatggcac
     7441 caaatgttgc cggtgttgtt ggttcagcag ttgctgctgg tgtcctttta ggaattttag
     7501 ggaaatgaat aagcaagtaa acaaacaaaa taataggagg caaaaatgcc aaaagtcctt
     7561 attgcaaccg aaaaaccctt cgctgcagat gcagttcaga aaattaaaac tgaactcgaa
     7621 aatgctggtt ataattactc ctttttggag aattataccg aaaagagtgc actgcacaat
     7681 gctttagctg atgcagaagc tttaattgtt cgtagcgata ttatcgatga agcagctttt
     7741 aatgctgcac caaaactaaa aatcgtggtt cgtgccggag ccggttatga caatatcgat
     7801 ctgaaatctg ctacagctca caatgttgtt gttatgaata ctccaggcca aaattctaat
     7861 gccgttgctg aactggcaat cggaatgatg atttatatgg cacggaataa gttcaatgga
     7921 aaatctggaa ctgaacttgc aggtaaaaca ctaactctat atggttttgg atgggtagct
     7981 cgttatgttg ccaaaattgc taaaggaatc ggaatggatg tttatgccta tgatcctttt
     8041 atttccgatg aagtaattca aaaagaaggt gttaaaccct tgcatagcgt agaagacata
     8101 ttcaaacttg gggattatat atcattacat attcccgcaa cggaagacac taaaaagagt
     8161 gtaaactgga acctcctatc tttagtgaag gatgatgcca ttttggtaaa tactgcccgc
     8221 aaagaagtta ttgaagaaga tgctttgctt aaagcttttg ccgaaaagaa aaaattccgt
     8281 tatgtgagtg atgtcgctcc cgataatagt gctaaattat tagaactcta tccagaccgg
     8341 gtgttcttta cacctaaaaa aatgggtgct caaaccagcg aagctaatac aaatgcaggt
     8401 attgctgcag ctaaacaaat tatcgcttat ttcgaaaaag gcgatgtaaa atataaagta
     8461 aattaactca atctctcctc ttatttcaac ggaaagctaa acttagcttt ccgttttggt
     8521 tttcagtaat tatatcccga gctgtatagc gcacaaatac ctctattggc ataaataata
     8581 atgataaggc aataataatg ataaggcaat taagtgaggg cagatattgc gtaaagatct
     8641 gttattaaag aaatagtgga tagcaacaag ctatccacct atttgtttga tatccttcag
     8701 cacatacaag ggataccctc agatcatatt cctactcttc gatattatgt ttaacattga
     8761 tgttatagcg ttttaattta tcgtaaaaac tgctgcggct gatgcccaag agtttcattg
     8821 ttaagctggc gttaaaatct gtattagtaa gaactttttt gatgaaatat gcctcacaat
     8881 cgggacaaaa agttccttta gttttggtaa cattttgttc aactggcggg aaaaggtctt
     8941 ctttgagtat ttcattgctt tccaggttca aaattgcttt tcgcaggatt tttttcagct
     9001 cacggatatt tcctggaaaa gaataagtaa gaatggcctt ataggcatct ttagtcaaaa
     9061 caggtatctt ggcgatacct tcttcttttg ccaattgcgg gataatatat tcgcacaaag
     9121 cagaaatatc ctcttttctt tcccgcaagg gaggaatatt tatttccaaa tcggaaatgc
     9181 ggtaaaatag gtcattccga aaggactgtt gttcaacttt ttgattcagg ttttgcattg
     9241 tggcaaaaat aaaacgagct tcggttcgag aaggtcggct ggaacccact ttataatact
     9301 ctctggtttc tattaagcgt aaaagtttta cttgctgttc cattggcaag tcaccgattt
     9361 catccagaaa aatagttccc ttacctgctt tttccaccag ccctgtagta ttggtgtttg
     9421 cacctgtaaa ggcacctttc acatagccaa ataaagtgct ttcaaaaagt tcgtgaggaa
     9481 tggcatttac attaacggca acaaattctt cttcagaacg gttgctgaaa taatgaataa
     9541 gcttggctat aatttctttt ccggttcccg attcgccggt gattaaaaca gaatggttat
     9601 tttgggcata ttttttggca agagataggc attttttcat ttgggggctt ttggcaataa
     9661 ctttagtccc taagctttgc tccatttttt ggctgataac ttgtgcttcc aattgatgca
     9721 aacgcgaaaa accgcggctg taatattcgg cgcgtttaac cgctttttcc atttcttcaa
     9781 gtgaataggg cttttccaga aacatcaggg caccctgatt ccaggcggta attacttttt
     9841 ctacggtggc gtgtcctgaa atcattatca cttcagtatc cggatatttt tgtttaataa
     9901 gtgacagaac atccaagcca tcttttttac gatcaaaacc gggtaaaacc acatccaata
     9961 ttatgatatg tacgatagat ttggttagaa caagctgtgc ttcttcccaa ttggaagctc
    10021 cgctgatgcg ataatttcgt tcgctgaaat aatggatcaa gccatcgcgg atactggttt
    10081 catcatccaa aacaagtata tgggttaaat cattgctttt cataatgatc ctctacagga
    10141 aaactgagtg aaacagaggt tccttttccc agttcacttt ccattgtcat attaccattg
    10201 ctgtttaaaa cgatgctatg tgccaaggat aaacccaatc ccgttccctg gccaatttct
    10261 ttggagctga aaaatggctc ggtggctctg cttaaggtct cttgatccat tccacaaccg
    10321 ttatcttcta ctaccagcat tatcctgcca tctataaact tggttgaaat tgtgatatat
    10381 tttccctcgg agcgattaac attcttaaga gcatcattgg aattggttgc cagaatggat
    10441 aaaagctgaa taaacctgat gttatctgca taaacaggag gtaaatctgg ttcgcagtcc
    10501 ttgatcagca aaacatcctt gctgattatt ttatcgccaa aaagttctat aaactcttct
    10561 ataatttgat tcaaactaaa agaattggcg ttgaaccttt cgggacttct ggcaataact
    10621 ttaatgattt ccaccaagcg attgatacgc tccaataatt gtagcacatc atttttcttt
    10681 tgtgaaagat aaccccttgg ctgcaaacta tttaagtcct ccaaagtaat ttctacaatt
    10741 tgttttatag cagccaaggg attgttaatt tcgtgtgcga tgcctgcaga aattttaccc
    10801 aacatagcca attgttcttg ttgagccagt aatttgtcct tttcttttat tttattcagc
    10861 tctgcttgga cgcgcttttc caaaagttca ttctgaaact gaagcatttc tttttcatcc
    10921 tgaaggcgag ctgtttcaaa actaatctgc ataaagcggt taattttctc ccgttcctga
    10981 ttgataaatt ccttgtagag ttgtgaatgg agatttaaaa agtaatttgc cttcttataa
    11041 tttcgtaact ttgtatagag tcccgcagca tattcataga tggaaatttg cacttcggaa
    11101 acggggttgt cttgcacttt ttttagcaat ttttttaata gggcttcggc atatttatag
    11161 tcctgttgtt tgatgcaagt taaaagaagg tctgcctgca gatttatccc catctccaga
    11221 tgttcatcag cagaaattat gtttaatgct tgtaaaatgg ttttcctggc aagtgcgtaa
    11281 cgatgcatat ctatataagt aagggctata gttcgtaaag aagcgctctg attataaata
    11341 tctccggttt gcaaatcata ctttaatgct tctttatatg cttttagggc ttctttcagc
    11401 tttccttgct tacgataaat atcggcaata ttacttaaat tacctgaaat ataataggga
    11461 atctgctgtt cccttccaat tttaagcgat ttataataat attccaatgc tttatcataa
    11521 tcctgcaact cctgataagc aacccctaaa gagcaatgga cctcgcctat cagataatta
    11581 ttgccggtgg attctgccaa agttaatgcc ttcagacagc aggcaaggtt tttattgtaa
    11641 tccattaaag atgaatagat ggaacccatt atgcaaagag aactaacttg caaatccaga
    11701 cattgaatgc tttccgcaag atctgctgcc ttttgataat actgaattgc ttgggcataa
    11761 tccattttag ctttatagat atttgccata ttagaatagg ttatggcaat actatgctgg
    11821 tcgttttcct gttcaaaaag agggattact tccataaagg tcttcaatgc ctgggcagtg
    11881 tttcccaaag tggtattcat agaagcaata gaggcaagcg attgtgccat ttgcagattt
    11941 ttgcctattt gagaaaataa atttaccgct ttctgataat aagggattgc ctcctgatgg
    12001 ttgcctaaaa cggaatttac cgatcccatc gttttattgg caaaggcaat ttcttcagcg
    12061 gttcccataa gattagccat ttgcaataat atttgggtag attcctctgc cttatgggga
    12121 ttgcaaccga tatagtaact tactaattta agcccttggt aaaaattatc ttttgtcggc
    12181 ttgccggata atgctttttc taaaagctta atttcctttt cttccattta ttcctcgagt
    12241 tatgttattt attcattttt ttctgcgtat ggcattttgc aatagatgaa cacataactc
    12301 ttttccaaaa ctttggtaaa attgtcaaga atagatcgca agatgtgttc caaaactgaa
    12361 taatgatact atatctttat ttatgaataa cttacgaata gaagaataga aggcagttat
    12421 ttattgcgtt gggtaaccga acaggtatat cacgcataac taactattta ataagttata
    12481 acaagccata caagtgttct ctatatacag gttaggagtc attggttaca gacagaacgg
    12541 aaccctaaat gtccccctgg caaccaaatg acgaaacttc ctgtagattt tacagtggtt
    12601 aatgtgggta tcaacggaat tttgatgtta ggaattgccg taataagtcc catttgcctt
    12661 gaaagttcta atatattcat tagttattaa agtacaaagg acacctaact gagaataatt
    12721 taactttttc gaatatgttc aaaaataatt cttatatgtt cttcggaaac gggtaatgga
    12781 ttcagagcca tatttctact acccataact tgtggaattg cctcttctat gaaggtatcg
    12841 cttagctcta tttttcgttc aaagggcaga gctcttagcc aggcataaaa tgcatcttta
    12901 ctaagattaa ggtatgcgaa caagtcattt agttctttag aaacggcagg ataatatagt
    12961 tccataatat cttccataac catagcgttt gctaaaccat gtggaatatt gtaattggaa
    13021 gttaaaggat aaccaatgga atgttgaaga gtagtacttc cttgagcaat aactattcct
    13081 ccgtaaagag caaactgcat caattctttc cttgcggcta aattatctgg ttcggataat
    13141 gcccttggta agcatttcat tatactggca attcctttat aaataaaggg ataaaccaga
    13201 ggtgaacgct gattgctata cagaccttct aaaagatgac ttaaagcatc caaagccgta
    13261 tgcatagtta cttttggcgt taaagttaaa gtgtatttcg ggtctatagc agccaacgcg
    13321 ggaaaagcta aatcactacc caagccagct tttttcttat gcgtgggatc ggataggacg
    13381 ctatattgag ttgcctcaga tcctgtccct gcagtaagtg gtatagccat aataggatat
    13441 acattcttga aggcagcaat gttatatatc tcatcgcggt tcaaagaatt cgcggcagcc
    13501 aaagatattg ctttagctgc atcaataggg ctacctcctc cgatgccgat tacaaaatca
    13561 caattatttt gtaaaaagat ttgcttgcct tccatcacgc tatccagggt gggattttct
    13621 ttaatgcgat caaaaaggac atagtgtatt tccagttcgt ttaaaactgt tatcagatca
    13681 gatagtgctc cactttgttt agcgcttttt tctccgctaa ccaataatgc cttttttccc
    13741 aaaacggcta tcttatcttt tgccttttga atagcatcat cggcaaagat gatgcgagtt
    13801 ggacaataaa acatttgagt acctacttta agagagttat ttttttattt attacaccgt
    13861 taggatgttg caaacgcaga aaatatacac ctgaagctag gtagcgtcct tgtgaatcac
    13921 aaccgttaaa actgatacta tgctttccgg cagtcatttt ttcctttgct aatgttgtca
    13981 ctttctgtcc tttcaggtta tatagttcac attctatata tccgctaacc ggaaggtaaa
    14041 aagagatggt tgtggaaggg ttaaaaggat tgggcataac agatatagat acttcaggca
    14101 tagccactgt ttcatccgta ataggtaaag cgggattaag ggttggcaga agctgatgca
    14161 acataattct tacttctgga tactgcataa aatacagagg aaaaccaaac attacaaaag
    14221 tgccgttaaa atcgtgcttg aaagcaatac actgtcctgc tccattactg ccatctatca
    14281 tattggcagt ataaattggt tcagcggcat tgttgaaagt ataaatgtag ggaagcattg
    14341 aattccagga aacaatggtt ttatcgggat caaccgttaa attatgatat atatcagaat
    14401 gagcactgat taaac
//
LOCUS       DAORHB010000012        14283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3301_length_14283_cov_4.427889, whole genome shotgun sequence.
ACCESSION   DAORHB010000012 DAORHB010000000
VERSION     DAORHB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_14283_cov_4.427889"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKM80_00810"
     CDS             <1..279
                     /locus_tag="PKM80_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92477.1"
                     /translation="ANEDPLYQRLITGWKYFDETIYGVGAEYTYLNLISLRGGYYSDK
                     EGEVEGASFGVGVEYKFSKRYKLGFDFAMVPAGGLTDYNKIFSLGFEF"
     gene            511..3612
                     /locus_tag="PKM80_00815"
     CDS             511..3612
                     /locus_tag="PKM80_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV92478.1"
                     /translation="MVISILPAQKMFLSKTGEYSKLLKHPYLDQLIQSNSKLDAIRKD
                     SANYNRLLVILVDFTLEEPDDFNTTGNGKFQLEPDPEYIYSIGAPPHNRQYYEANLDA
                     LKYYYLAVSAGSYELQYDVYPKDKVAYTLPKSMGYYNPPGASSELFVSRMEEYFKTAF
                     ELADSLDEEIDFASYSHYMIIHSGSDWQHDISANTPSDLPSFYIRVGDGKEAVVDNGT
                     VLISHSCNVPETISQDFYISQSNGQNIHNGYGALNSVIAHEFGHSLGMVDLYNVYNSS
                     PMVGVFDIMDSGGSGILVDELENGDIVYVEGALPTLPGAFSRNLMFRDNYLERGLLKE
                     FPDFPVSSELSLNAISAMQNTTDPVPHTYKIPLNAKEYILVENRNVDPDGDGGTAVYG
                     ALDGRVVLYPTPFEDITPPPPTYEYDYLLPSFIKTNGSAIGGGILVWHINEAILYDEG
                     QTDSFGNWTSNFDANTVNTNFYRKAVSIIEADGLTDLGSEYSMYWTGTPYDYFHTHKP
                     ILDSGGLFVNWSQELWKPELTPNTKPPLVDSFNMPGFYHLKQISNPSRKMSFRVESGL
                     FDSTQILDYDSATTITGPVINSGFSEWNIPVLKTGNVNLLSNNNGIWQDLMGDYTIPF
                     SSTDYPLQRMDINQDGFWELVGIENNQLYSIDWANDDLIVKTITFPNPPTTTPLSMDN
                     SLIVGTDEGIYALNNFVEPYYVSIRGIKCLAGYDDKIIALGENYFKIFSTVDLSLISE
                     FSLPESFGNIEPLIYTNPDRTITMIFVMSNSGNLYRLYQNRLELIFQNHSNNTPGQLA
                     CTSLGDISPVLFFGIGNQFYALKADGTKLTNFPIVSYDTVSSWESPMALQLNVDETLF
                     YPLRGSGYLAVDKYGKIVPDMSLSVNREHKSDYLFYNQQQQSLCWYYTDNSGKLYIHT
                     KNGISTNPILFAGFRNGASGCAIFDFKDESTSSTEKYAYIYPNPVRKNYFKINLQNYY
                     GQTKLWLYDISGTLIRNLVIPETLNNPRDFELSTKGLSSGVYIMILENGCTTKQLKFA
                     IEK"
     gene            3631..4599
                     /locus_tag="PKM80_00820"
     CDS             3631..4599
                     /locus_tag="PKM80_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92479.1"
                     /translation="MKKTALLSLLLIFSFCLFGQNKQETDIDQWLYQTEEENIDASRD
                     TVITNDLIKVNYNQKSAHLAMAMSMLIPGTGQFYANRTAITTYIFPILELGFIGGIVY
                     YNKQGNDKTDKYEKYANGELVTYLTPDGTPISGYRYERGRQNQIQDILKDINTVDIYE
                     DTYFRLDENNSQHFYEDIGKYPHYVFGWMDWYYRFAADESGNFVTPRWQFDGPNDSPH
                     THWTGNYPICGQNTDIAISINSHEMSRMRQTYIKMRNDAKDDYGIAHLFTFGLAFNHL
                     VSGLDAVRVTRNFNRYYISQAAPSFNFYAAMPSGNVTPMVGFKWMF"
     gene            4587..5246
                     /locus_tag="PKM80_00825"
     CDS             4587..5246
                     /locus_tag="PKM80_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92480.1"
                     /translation="MDVLKYKFWLIVLIVLSASIPLLALPKIVYPVSSLILPGSGELL
                     LGHNTRGATLLGADLVNLYAFYATDKEIDLQKENYMKFAELYAGVPYGMPKQHYQAVQ
                     NYPSSSYYNNIQDMMARNYYLIYNYDPQSYTDYIAANTYQGDEEWNWQSEDKWNQYKA
                     IRKRHQKAKMNNQLCLGLLLLNRCLSVIDSSILSKKEMGNVYLSPLSNEGAMLHYQID
                     F"
     gene            5257..5904
                     /locus_tag="PKM80_00830"
     CDS             5257..5904
                     /locus_tag="PKM80_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92481.1"
                     /translation="MKRIYIFLLIILFAAMLFAENRSLMVLKSLAVPGLSQINSGKEY
                     GYVMLTTEALIIGSLFYFNSESDILKQESYEYAIKYAHLAPGHYSDTYFKHLARYESS
                     GFDAGGYNNWIRQTALELYPYDPAMQQQYIAENCYTDDQYWYWDSSENRNQYNKIRNR
                     SNDYRDYAMVTGGILLLNHLTSAIDVMRWSAEAKRGNISFSLKERTPILQLNYQW"
     gene            complement(5918..6400)
                     /locus_tag="PKM80_00835"
     CDS             complement(5918..6400)
                     /locus_tag="PKM80_00835"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNV92482.1"
                     /translation="MIKLSELPVSYGKVHLPAKVALVVTQKPTSGYNLITIEWFMRTS
                     LQPPMFAISIGESRYSHECLQANRYFNLVFPSLKMKQLLSLCGSNSGRDMDKFTVAKV
                     EYIQGKLNKLPVLKDAVACFECEVVSEIRSGDHTIFIGQVHYSWQNEEEELFYYQEKL
                     "
     gene            7178..7618
                     /locus_tag="PKM80_00840"
     CDS             7178..7618
                     /locus_tag="PKM80_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MraZ"
                     /protein_id="HNV92483.1"
                     /translation="MSGEFLGIFENSVNKQRVIIPAAFKKKFSEESEKKVIVTLGPND
                     TIAIYPLDNWNETLERLRIGDEHSNKLRTQLIDFAMTEQELEGPGRIRILERLLNEVN
                     ITDSVIIKGEGHYISLWNPKVYNEVRASKLNQHRKEFTSEDYQL"
     gene            7615..8613
                     /gene="rsmH"
                     /locus_tag="PKM80_00845"
     CDS             7615..8613
                     /gene="rsmH"
                     /locus_tag="PKM80_00845"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNV92484.1"
                     /translation="MIFHTPVMAEKCISYLNLRAGKIYVDATTGGGGHSLAMLKSEPS
                     IRLYCFDQDMEAIEEASKKLSNYENVELIKANFQQLRTELAYRKVKGIDGILFDLGVS
                     SHQLNNENRGFSFEKDAPLDMRMDSSQEYSAYDAVNELDAKELARIFRDYGEELNAFR
                     IAKAIERSPKPVNTTGELTRIIESVVGKGTKASLKTKVRIFQALRIYVNKELEVLAST
                     LKDAINLLNPKGRIVVLSYHSLEDRIVKQEFKRAEQECICPPRSLACNCNHHKQLVIL
                     TSKPITASEEEILANPRSRSAKLRAAEKVKEENKEEKTYRKSNRQKTQEEKCVAKS"
     gene            8595..8951
                     /locus_tag="PKM80_00850"
     CDS             8595..8951
                     /locus_tag="PKM80_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92485.1"
                     /translation="MRGKILILVSLLGVVFFLSFYNNNRIVQYTRQISELEKTYYAEK
                     NINTELLVELDDLRSGRHIASLVRVELSNFIPDQQAGKIIYVHEPSAKQDKKTYCIID
                     LLTTKAEAKNIQVLLD"
     gene            8953..11127
                     /locus_tag="PKM80_00855"
     CDS             8953..11127
                     /locus_tag="PKM80_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV92486.1"
                     /translation="MKSRFYFLVVIFGLASIFWVAYLFCIQILDPFHLAGVRRMRYTP
                     KKEILIPRRGSILDSQGNLLVSSISYYQIDIDRSSVAMWAKDNNLTLEDAFAKYAELI
                     AAHTSLNKDEVLKRLTIGNKNTSVQISNKISEVELDKLIRDFKSKEMIGLIHSFASMR
                     RIYSKDILAARVMGSVREVSDGYDPATHSKSLYQLSGVCGIESTYDKYLSGNYGWKEI
                     VLDANHRPVPYPNLHSKKPQNGYNVYLTIDSKIQEIVENALFEGMEQYSAKNAGAIVM
                     DPNTGKIIAMAGVSRDDRIEDPNLVRVKTNIPVSFMFEPGSTMKPLTMLPAIEYKLVK
                     PTETFQCGRYQTGRRVISDTHQYGNLTPREIIVKSSNVGIAKIAERIGPTRLYEKFIA
                     LGFGQKTALNMTGESSGMFAKLQNWDGYTLHSVSFGQGISTTALQLATAYCAIANGGK
                     LMKPIIVDTIRDEEGKIVEQFEPTVLRNVSTPAATDTIKSYLQGVVDNGTAKHIKMDY
                     IQVGGKTGTAQKNITGTKGYSEGKYSSVFVGMFPIEDPQMVIVVFYDEPAPGFHFGST
                     SSAPTFKKIVENILFMPDCHILPYNERLMQTSLTMPKLIGMHLFQAERTLNYYGFQYK
                     VEGPDSASVVIDQYPKEGVTVDPHYPITLKIGPSADKKAPVREAGIMPNLIGLSLREA
                     LKQASAHCLAINIRGSGIVRSQSILPGSRILEGSKCHLEASL"
     gene            11124..13973
                     /locus_tag="PKM80_00860"
     CDS             11124..13973
                     /locus_tag="PKM80_00860"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNV92487.1"
                     /translation="MINKIINALKQNSLLIETANLEQLQNWQGKVQTDTRKLQPGDIF
                     VCIKGTNYDGHNFIPSALNKGAALIVGQEFDNITFPYLKVKDSRKAAAIIARIVLLPE
                     KTPFTLIGITGTNGKTTTSIMIYEAIRDLGFRCGWIGTLGYYIEGRTFPSERTTPDII
                     ELNEIFAQMIDERVNYVVMEVSSHSLSLNRVYGVKFDYCLFSNLGRDHLDFHLTIENY
                     GQTKMSFFEQGAKDGSVSIVNTGDPFGIRIKEMLVEKHAKVLSLGSEDADFRFADVQT
                     DINNSSFRLQSDKDEIYIISRLIGNFNIQNLGLTALTLYAMGFKPEEIHKSIKDIPPV
                     RGRMEKVENNYGIGIFIDYAHTPQAIENVLEAGEDLPHKRMLCLFGAGGDRDKGKRPL
                     MLKAALNHSDVVIISDDNPRSENPEAIIRDIVSSSDIFLPWWIIRDRKQAIESIIRFA
                     QKDDIVLICGKGSENYQEIEGVHYHFDDLETTLEALAKRDTKIKDDAEFILPVDQTLM
                     KLLFISNWNPEPSGYKEPVYYKYLYTDSRILKPESIFVAIQGEKFDGHNYLKEVLAPK
                     ENCAIGEKSWSEESPIAEFQYFQVNNSLEAMGSICRKYLALFAPHKIALTGSTGKTTS
                     KELMAQILESSAPTLKTLANENNMIGLCKTILRIKPEDRYALFELGTNHFGEIASLAD
                     VVDPEIGIILNIGPSHLEYFKDEDGVFQEKIALFNRPLALRLYPGDDSRFQYFAKQGL
                     SIGFNENCDYRITQHQILDSSQSFYLNEDLWIIPYGAPYFALNAAFAIVLCLKLGISK
                     KDIQSALNSPIYLELRNQVVKRGTGILILDCYNANPYSMQKALEYWQTLSSEQPHYAV
                     LGDMLELGNSSVMYHKMIAAMLSEMRYEKLITVGSFSRLFHPDRENAILHYNNVEELI
                     ASGNLRTFPNNAIILVKGSHSIQLERIIPILKGEN"
     gene            13973..>14283
                     /locus_tag="PKM80_00865"
     CDS             13973..>14283
                     /locus_tag="PKM80_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNV92488.1"
                     /translation="MFYHLLYPLAKYSIVFNVFRYVTFRSIGAFITSLIISLLVGPTF
                     IKMLRKRSAVETIDEDLPEKHRLKQGTPTMGGIIILFSLLISSLLWNVLTNPAILMMY
                     LA"
BASE COUNT         4720 a         2457 c         2659 g         4447 t
ORIGIN      
        1 gcaaatgagg accctttgta tcaaagattg ataaccggat ggaagtattt tgacgagaca
       61 atatatggag taggtgcaga gtatacttat ctaaatctga tctctttacg aggtggttat
      121 tattctgata aagagggaga agttgaagga gccagttttg gagtaggtgt tgaatataaa
      181 ttcagtaagc gttataagct tggtttcgat tttgctatgg ttcctgcagg tggactgact
      241 gattacaata aaatattctc tttagggttt gaattttaac tcctaataaa ataatgcaaa
      301 aaaaaatata taggtagttg tttgatgagg gctgctaagt ctcttaagaa tggaagtcct
      361 cgtcgaacac ctttatctgt tgatataatg ttattgacaa tttttttgat aattcaatat
      421 tagagtagta ttggttatat ataaagtaac ctcgaacaca ggatcaaaat taataatatg
      481 aaaaagctat cattactgct actatcccta ttggtaatat ctatattacc tgcccaaaag
      541 atgtttttaa gtaaaactgg cgaatatagt aaactactaa aacatcctta tttagatcaa
      601 ttaattcaat ccaattccaa actagatgca ataagaaagg attctgccaa ttataaccgt
      661 ttgttggtaa ttttggttga ttttacttta gaagaaccgg atgatttcaa taccactggc
      721 aacggcaaat tccagcttga acccgatcct gaatatattt attcaattgg agctcctcct
      781 cataacagac aatattatga agccaatctg gatgccctaa aatattatta tttagctgtt
      841 tcagcaggtt cttatgaact ccaatatgat gtatatccta aagataaagt tgcttataca
      901 ttacctaaaa gtatgggata ttataatcct ccgggagcaa gttcagaact atttgttagc
      961 agaatggaag aatatttcaa gactgctttt gaattagcag atagtttaga tgaggaaatt
     1021 gattttgcca gttatagcca ttatatgatt attcattctg gatctgattg gcaacatgat
     1081 atttctgcaa acactccttc cgatcttcct tctttttata tcagagttgg cgatggcaaa
     1141 gaagcagttg ttgataatgg tacagtcctt atatcacatt cttgtaatgt tcccgaaact
     1201 atctctcagg atttttatat cagccagagt aatggacaaa atattcataa tggctatgga
     1261 gctttaaatt ctgttatagc acacgaattt gggcactcct taggaatggt agatttatac
     1321 aatgtataca attcttctcc tatggtaggc gtgtttgata ttatggatag tgggggttcc
     1381 gggatattag ttgatgaatt agaaaatggg gacattgttt atgtggaagg tgccttacca
     1441 acattaccgg gggctttttc tcgcaattta atgtttcggg ataattatct cgagcggggg
     1501 ttattaaaag aatttcccga ttttcctgtt tcatcagaac tatccttgaa tgcaatcagt
     1561 gctatgcaaa atacaactga tcctgttcct cacacttaca aaattccact aaatgccaaa
     1621 gaatatattt tagtagaaaa ccgcaatgta gatcctgacg gtgatggagg tacagcagtt
     1681 tacggagcat tggatggaag agttgtttta tacccaactc cctttgaaga tataactcct
     1741 ccacctccca cttatgaata tgattatctt ctaccttcat ttataaaaac taacggatca
     1801 gcaataggtg gcggaatctt ggtttggcat ataaacgaag ctattctata tgatgaaggc
     1861 caaactgata gctttggtaa ttggaccagt aattttgatg ccaatacagt gaatactaat
     1921 ttttaccgaa aagcggtttc aattattgaa gctgatggac ttacagacct aggtagtgag
     1981 tattctatgt attggacagg cacaccttac gattacttcc atacacataa accaatactt
     2041 gattctggtg gtttatttgt caactggagc caggaacttt ggaaaccaga acttactccc
     2101 aacacaaaac cacctttggt ggatagtttt aatatgcctg gtttttatca tttaaaacag
     2161 ataagcaatc catctcgtaa gatgagtttt cgggtagaaa gtggtttatt tgatagcact
     2221 caaattctgg attatgattc tgctaccacg ataacaggtc ctgtaataaa ttccggattt
     2281 agcgaatgga acattcccgt gttaaaaaca ggaaatgtaa atttgctaag caataacaac
     2341 ggtatctggc aagaccttat gggagattat actatcccat tttcttctac tgattatccc
     2401 ttacaaagaa tggatatcaa tcaggacggc ttttgggaat tagtaggcat agagaataat
     2461 caactttatt ctatcgattg ggctaatgat gatcttattg taaaaacaat cactttccct
     2521 aatcctccta ctactactcc tttatctatg gataattcgc ttatagtagg taccgatgaa
     2581 ggcatatatg ctttgaataa cttcgttgaa ccgtattatg tttctattcg aggaataaaa
     2641 tgcctggcag ggtatgatga taaaataata gctttaggcg aaaactattt taagattttt
     2701 tccacagtag acttaagctt aataagtgag ttttctcttc cagaatcctt tggcaatatt
     2761 gaaccactaa tttataccaa tcccgataga actatcacta tgatttttgt tatgtctaat
     2821 agtgggaatc tatatcgtct atatcaaaac cgactggaat taatctttca aaatcacagt
     2881 aataatacac ctggccaatt agcttgtact tctttaggtg atatatcgcc agttttattc
     2941 tttggtatcg gtaaccaatt ttatgctcta aaagctgatg gaaccaaact aaccaacttc
     3001 cctattgtat cttatgacac agtttccagt tgggaaagcc ctatggcttt acaacttaat
     3061 gtggatgaga ccctattcta tcctttacga ggtagtggtt atttagctgt agataaatac
     3121 gggaaaatag ttccagatat gagtttgtcg gtaaatcggg aacacaaatc agattatctt
     3181 ttttacaatc aacagcaaca atcattatgt tggtattata cagataattc cggtaagcta
     3241 tatattcaca caaaaaatgg tatatcaacc aatcctattc tttttgctgg tttcagaaat
     3301 ggggctagcg gctgtgccat ttttgatttt aaggatgaga gcactagttc cactgaaaag
     3361 tatgcctata tatatcccaa tcctgttcgc aaaaactatt tcaagataaa tcttcagaac
     3421 tattatggcc aaacaaaact gtggctttat gacatcagcg gaactttaat tcgcaattta
     3481 gtaattcctg aaactctgaa caaccccaga gattttgagc tgagcactaa gggtcttagt
     3541 tccggagtat atattatgat tttagaaaac ggttgtacaa caaagcaact taagtttgcc
     3601 atagaaaaat gatctattaa ggagttcaca atgaaaaaaa cagctttact atcgcttctc
     3661 cttatcttta gcttttgtct atttgggcaa aataaacagg agacagacat tgaccagtgg
     3721 ctgtatcaaa cagaagaaga aaatatagat gcatcccgag atacagttat tacaaacgat
     3781 ttaataaaag ttaactacaa tcaaaaaagc gctcatttag caatggcaat gtctatgttg
     3841 attccaggta caggtcaatt ttatgccaat cgaacagcta ttacaactta tattttcccg
     3901 atattagaac ttggttttat tggtgggatt gtatattaca ataaacaggg taacgataaa
     3961 accgataaat atgaaaaata tgccaatgga gaacttgtta cttaccttac tcctgatgga
     4021 actccaatat caggatatcg ttatgaaagg ggaaggcaaa atcagattca ggatatcttg
     4081 aaagatatta ataccgttga tatatatgaa gatacctatt tccgcctgga tgagaataat
     4141 tctcagcatt tctatgaaga catcggaaaa tatccacatt atgttttcgg atggatggat
     4201 tggtattacc gttttgcagc tgacgaatca ggaaattttg ttactccacg ttggcaattt
     4261 gatggcccca acgatagccc tcacactcac tggacaggca attatcccat ttgtggacaa
     4321 aatacagata tagccatttc tattaattca catgagatga gccgaatgag gcaaacttat
     4381 attaaaatgc gcaatgatgc taaagatgat tatggtatcg cccatctttt tacttttggt
     4441 ttagctttta accaccttgt atcagggctt gatgctgtcc gcgtaaccag aaattttaac
     4501 cgttattata tttctcaggc agctccttcc tttaatttct atgcagcaat gccttcagga
     4561 aatgtaaccc ctatggtagg ttttaagtgg atgttttaaa atataaattc tggctgattg
     4621 ttttaatcgt attatcagct tctattccgc tattggcttt acccaaaata gtatatcctg
     4681 tttcttcttt aatattaccg ggaagcggag aattactttt gggacataac acaagaggag
     4741 caactttgtt aggagcggat cttgtaaatc tatatgcatt ttacgctaca gacaaagaaa
     4801 ttgatttgca aaaagagaac tatatgaaat ttgcggaact ttatgcagga gttccttatg
     4861 gaatgccaaa acagcactat caggcagttc aaaattatcc cagtagttct tattacaata
     4921 atatccaaga tatgatggca agaaactatt atctgatata caattatgat cctcaatctt
     4981 acactgatta tatcgctgct aatacttatc aaggtgatga agaatggaat tggcaaagcg
     5041 aagataaatg gaatcaatac aaagctatcc gcaaaagaca tcagaaagcg aaaatgaaca
     5101 atcagctctg tctaggatta ttacttctta atcgctgcct cagtgttatt gatagctcca
     5161 ttctatctaa aaaggaaatg ggtaatgtat atctatctcc tttatcaaac gaaggcgcta
     5221 tgctgcatta tcaaatagac ttttaggagt aacattttga aacgtatata tatttttctg
     5281 ctgatcattc tttttgctgc aatgttattt gcagaaaacc gtagtttgat ggttttaaaa
     5341 tctttggcgg ttcctggctt gtctcaaata aattccggaa aggaatacgg ctatgtaatg
     5401 cttactacag aggccttaat tattggctca cttttttatt tcaatagtga aagcgacata
     5461 ttaaagcagg aatcttatga gtatgccata aaatatgctc atcttgctcc cggacattac
     5521 agtgatacat attttaagca cttagcccgt tatgaaagta gcggttttga tgccggaggc
     5581 tataataact ggattcgcca aactgctctg gaattatatc cttacgaccc cgctatgcaa
     5641 caacagtata ttgcagaaaa ttgttataca gatgatcaat actggtattg ggatagttcg
     5701 gaaaatcgta atcagtataa taaaatacgc aatcgcagta acgattacag agattatgct
     5761 atggtaacag gaggcatttt actactaaat catctgacaa gtgctattga tgttatgcgt
     5821 tggagtgcag aagctaagcg aggtaatatc tctttttctc ttaaggaaag aacacctatc
     5881 cttcagctaa actatcaatg gtaaaccttt ttctgtatca gagtttctcc tgataataga
     5941 ataattcctc ttcctcattt tgccaactat aatgaacttg accaataaag atagtatgat
     6001 ctccgctgcg aatttcagat acaacctcac attcaaaaca tgcaacggca tctttcaaaa
     6061 ctggtaattt attcaattta ccctgaatat actccacttt ggcaaccgta aatttatcca
     6121 tatctctccc tgaattcgag ccacacaaag ataaaagctg cttcatttta agcgatggaa
     6181 agaccaaatt aaaatatcga tttgcctgaa gacattcatg agaatatcgg ctttcgccaa
     6241 ttgatattgc aaacatcgga ggctgaagag aagtgcgcat aaaccactca atcgttatta
     6301 gattataacc actcgtcggc ttttgcgtta ctaccagtgc tactttggct ggtaaatgaa
     6361 cttttccata acttacaggc aattctgaaa gtttaatcat cttatctcct aagcaatatt
     6421 agttatatat attatagccc aaatttgaag ttttgcaacc tcaagattga ttttccctcg
     6481 gcctgttaat agatacagct ttgcattctt agtgttatga atctgggctc aaaacctgcc
     6541 attattttcc ataatgctat cgaatatagt aagatccatt tacaataatc cgctgctata
     6601 catagaacct acaaaattca aagggaataa cagtatcact tttatgctgt ttattaaaaa
     6661 ccaatcgtcc aatttccttt atgaaaatgg gcatagtatt ctaaaaatag agataagctt
     6721 acccagaata tctgcaaaat cggattattt tgaagaagcg ccaaaaaaat attttcccgc
     6781 ccaaaatata gctgtaagct tgtaacaatg cggaaaattg catagttaca gcttacttta
     6841 aagaaaaaat attttaagaa aataacaact tattgcctgc caaatagtta agcccctaaa
     6901 cttgataaat gagcttaaga gtatatagcc ataactataa gtagtataaa atgataaact
     6961 ttccaccttg aaccctgatg ttgaaaaaaa tactctatct atttataaaa aaacgcattt
     7021 tgcacttgac aataaaaacg agatctcata taagaaccat agaaagtcat aaaaactcaa
     7081 aatatctcaa ggcggttcta aaaaatgagt caaaaaagca cggactttaa agaaaaagag
     7141 ctttataaag ggaaactcta aaaaaaggat ttcgagtatg tctggtgaat ttcttggtat
     7201 tttcgaaaat tcagtgaaca agcaaagggt aataattccc gcagcattca aaaagaagtt
     7261 ttctgaggaa tcggaaaaga aagttattgt tactctgggg cccaatgata caatagctat
     7321 ctatcctttg gataactgga atgaaaccct ggaacgttta agaattggtg acgagcatag
     7381 caataaactt cgcactcaat taatcgattt tgctatgaca gaacaggaat tagagggtcc
     7441 gggaagaatt cgtattctgg aaaggttgct gaatgaagta aatattacag atagtgttat
     7501 tattaaaggc gaggggcatt atatttcact ttggaatccc aaggtttata atgaagttcg
     7561 tgccagcaaa cttaatcaac accgcaaaga atttacttca gaggattatc agctatgata
     7621 tttcacactc cagtaatggc agaaaagtgc atctcgtatt taaatttgag agctgggaaa
     7681 atatatgttg atgcaacaac cggaggtgga ggacacagct tagcaatgtt aaaatctgaa
     7741 ccgtccataa gattatattg tttcgatcag gatatggaag caattgaaga agcaagtaaa
     7801 aaactaagta actatgaaaa tgtagaattg ataaaagcta atttccaaca gcttcgtact
     7861 gagcttgcct atcgtaaagt gaaaggtatt gatggcatac tttttgattt gggtgtttct
     7921 tctcaccagc tcaataatga aaaccggggt tttagttttg agaaagatgc tcctttggat
     7981 atgcgaatgg atagcagcca agaatatagt gcttatgatg cagttaatga attagacgcc
     8041 aaagaattag ctaggatatt tagagattat ggagaagagc taaatgcctt tcggatcgct
     8101 aaagccatag aacggagccc aaaacctgtg aatacaacag gcgaattaac cagaataatt
     8161 gaaagcgttg ttggaaaagg aactaaggcc tcgcttaaaa ctaaagtgcg tatatttcaa
     8221 gcattaagga tttatgtaaa taaggaattg gaagttcttg cttctacttt gaaggatgct
     8281 ataaatttat taaatccgaa aggtaggata gtggttctta gttatcattc tttagaagat
     8341 cgaatcgtta aacaagaatt taagagagca gaacaagaat gtatatgccc tccccgttcc
     8401 ttagcttgta actgtaacca tcataaacag ttagttattt taacttccaa acccataacc
     8461 gcatcagaag aagaaatttt ggcaaatcct cgttcgcgca gcgctaaatt gcgtgctgca
     8521 gaaaaagtta aagaggaaaa caaagaggaa aaaacctacc gcaaatcaaa ccggcagaaa
     8581 acacaggagg aaaaatgcgt ggcaaaatcc tgatacttgt gtccctattg ggagttgtgt
     8641 tttttctaag cttttacaat aataaccgaa tcgtgcaata taccagacaa atttctgagc
     8701 tggaaaagac ctattatgcg gaaaaaaaca tcaatactga attattagta gaacttgatg
     8761 atttacgtag tggaagacat attgcttctt tagtaagagt agaattgagc aattttattc
     8821 ctgatcaaca agcaggtaaa attatatatg tacacgagcc ttcagctaaa caagataaga
     8881 agacctattg tattatagat ctattaacta ctaaagctga agctaaaaac atacaggttt
     8941 tgttggattg agatgaaatc tcgcttttac tttttagttg ttatttttgg tcttgcttca
     9001 attttttggg tagcgtatct attctgcatt cagattttag atcccttcca tttagctgga
     9061 gtaagaagaa tgcgatatac acctaaaaaa gaaattttaa ttcctcgtag gggttcaatt
     9121 cttgattccc agggcaatct tttagttagc tcaataagct actatcaaat agatatagat
     9181 agatcttccg tggcaatgtg ggcaaaagat aataacttaa cattagaaga tgcgttcgcg
     9241 aaatatgcag aattgattgc tgctcatact tctttaaata aagatgaagt gttaaaacgc
     9301 ctaactattg gcaataaaaa cacttctgtt caaatttcca acaagattag cgaagtagaa
     9361 ctggacaaat taattcggga ttttaaatct aaagaaatga tcggtttgat tcatagcttc
     9421 gcttcaatga gaagaatata ctccaaagat atattggctg caagagtaat gggttcagtg
     9481 cgcgaggtat ctgatggcta cgatcctgca actcatagta aatctctcta tcaactctct
     9541 ggagtttgcg ggatagaatc cacttacgat aaatacttat ctggaaatta tggctggaaa
     9601 gaaatagtat tggatgccaa tcatcgacct gtcccctatc ctaatctgca ttccaaaaaa
     9661 cctcaaaacg gttacaatgt ttatcttaca attgattcta aaattcaaga aattgtagaa
     9721 aatgctctat ttgagggaat ggaacaatac agcgcaaaaa atgctggcgc tattgttatg
     9781 gacccgaata ccggtaaaat tatagcaatg gctggagtat ccagagatga tagaattgaa
     9841 gatccgaact tggtgagagt aaaaaccaat attcctgtta gttttatgtt tgaacctggt
     9901 tctacaatga agcctttaac tatgttgccc gctattgaat ataaactggt aaagccaacg
     9961 gagacatttc aatgcggtag ataccaaacc ggacgaagag ttatttcgga tacacatcaa
    10021 tatggaaatc ttactcctcg cgaaataata gttaaatcca gtaatgtagg tattgctaaa
    10081 atagcggaac gaattggccc cacgcggtta tatgagaaat ttatagcttt gggttttggc
    10141 caaaaaaccg ctttaaatat gacaggagaa tccagtggaa tgtttgccaa actgcaaaac
    10201 tgggatggct atacattaca ttcagtttct tttggccaag gtatatctac aactgcctta
    10261 caacttgcca ctgcctattg tgccattgct aatggaggga aattgatgaa acctattatt
    10321 gtggatacca tccgcgatga agaaggaaaa atagttgaac aatttgaacc cacggtttta
    10381 cgcaatgtat ctactccggc agctactgat actataaagt catatcttca aggtgtggta
    10441 gataatggaa cagctaaaca tataaaaatg gattatatcc aggttggcgg aaaaactgga
    10501 actgctcaaa aaaatattac aggaaccaaa ggatacagtg aaggtaaata tagttctgta
    10561 tttgtgggaa tgttccccat tgaagatccc caaatggtaa tagtggtttt ttatgatgaa
    10621 ccggctcctg gatttcattt tggcagcaca agtagcgctc ccactttcaa aaaaatagtg
    10681 gagaacattc tatttatgcc cgattgccac attttgcctt ataatgaacg tttgatgcaa
    10741 acctcattaa ctatgcctaa acttattgga atgcatcttt tccaggcaga aaggactttg
    10801 aactattatg gatttcaata taaagtcgag gggccagatt ctgcctctgt agttattgat
    10861 caatatccaa aagaaggagt tacagtagat ccacattatc caataacctt aaagataggc
    10921 cccagtgcag ataaaaaagc ccctgtccgc gaagcaggaa ttatgccaaa tttaatagga
    10981 cttagtttaa gagaagctct aaaacaagca tcagctcact gtttggcaat caatattcgt
    11041 ggttcaggaa ttgtccgctc tcaatctatt cttcctggga gtagaatatt ggaaggcagt
    11101 aaatgccatc tggaggcatc gttatgatta ataaaataat aaatgcctta aagcagaatt
    11161 ctctattgat agaaacagct aatttggaac aactgcaaaa ctggcaggga aaagttcaaa
    11221 ccgataccag aaaattgcaa ccaggtgata tttttgtctg tattaaaggc acaaattatg
    11281 atggccataa ttttattcct tccgctctta ataaaggtgc agcattaatt gtaggacagg
    11341 aatttgataa catcactttc ccttatctaa aagtgaaaga cagtcgtaaa gcggcagcta
    11401 ttattgctcg aattgtgttg cttccagaaa aaactccatt tactttgatt gggataacag
    11461 gcactaatgg aaaaactact acctcaataa tgatctatga agctatcaga gatttggggt
    11521 ttcgttgtgg ttggattgga actttgggtt actatattga aggtagaact ttcccttctg
    11581 agagaaccac tcccgatatt attgaactaa atgaaatatt cgctcagatg atagatgaaa
    11641 gggtaaatta tgtagttatg gaggtttctt cccattcact atctctaaat agagtttatg
    11701 gagttaaatt cgattattgt ttgttcagta atctgggtcg agatcatctt gattttcatc
    11761 ttactataga aaactatggt caaacgaaaa tgagcttttt tgaacaaggc gcaaaagatg
    11821 gtagcgtttc catcgtcaat accggcgatc cttttggtat aaggataaag gaaatgcttg
    11881 ttgaaaaaca cgctaaagta ctttctcttg gaagtgaaga tgctgatttt cgttttgctg
    11941 atgtacaaac tgatataaat aacagcagct ttcggttgca atccgataaa gatgagattt
    12001 atattatcag ccgcttgatt ggtaatttta atattcaaaa tttggggtta acagctctca
    12061 ctttatatgc aatggggttc aaacctgaag aaattcacaa atccataaaa gatattcctc
    12121 ccgtccgagg cagaatggaa aaagtggaaa acaattatgg tatcggcatt tttatcgatt
    12181 atgctcatac gcctcaggct attgaaaatg ttttagaagc aggagaggac ttacctcata
    12241 aaagaatgct ctgtttattt ggtgccggag gagatagaga taaaggaaaa agacccctaa
    12301 tgttgaaagc agctctcaat catagtgatg ttgttattat cagtgatgat aatccacgca
    12361 gcgaaaaccc tgaggcaatt atcagggata ttgtatcctc aagcgatata tttttaccct
    12421 ggtggattat cagagataga aaacaggcaa tagaatccat tattcggttt gcacaaaaag
    12481 atgatattgt tcttatttgt gggaaaggga gtgagaacta ccaagaaatt gagggggttc
    12541 actatcattt tgatgatctc gaaacgactt tggaagcact ggcaaaaagg gatacaaaaa
    12601 ttaaagacga cgcagagttt attctacctg ttgatcagac cctaatgaaa ctactattta
    12661 tttcaaattg gaatcccgaa ccttctggtt ataaagagcc agtatactat aaatatctat
    12721 atacagattc tcgtattcta aagcccgaaa gcatatttgt agccattcag ggagaaaaat
    12781 tcgatggcca taactatcta aaagaagtac ttgcccctaa agaaaattgc gctattggtg
    12841 aaaaatcttg gtcagaggaa tctcctatcg cagaatttca atattttcag gtgaataata
    12901 gtttggaagc aatgggttct atctgccgta aatatttagc tctatttgct cctcacaaaa
    12961 ttgctctaac cggaagtact ggtaaaacta cctccaaaga attgatggct caaattttgg
    13021 aaagttccgc tcccactttg aaaaccctgg ctaatgagaa caatatgata ggcctctgta
    13081 aaactatttt gaggataaaa cctgaagatc gttatgccct ttttgaactt ggaaccaatc
    13141 actttggcga aattgcttct ttggcagatg ttgtcgatcc tgaaattggt attattttga
    13201 atatcggtcc ttctcattta gagtatttta aggatgaaga tggagttttt caagaaaaga
    13261 tcgctctatt taatagacct cttgctttac gcttatatcc tggagatgat tctcgttttc
    13321 aatattttgc caagcaggga ttaagtattg gctttaatga aaattgtgat tacaggatta
    13381 cgcaacatca gattttggat agtagccagt ccttttattt gaatgaggat ttatggatta
    13441 ttccttatgg agcaccttac tttgctttaa atgctgcctt tgctattgtg ttatgcttga
    13501 aattgggaat cagcaaaaaa gatatacaat cagctttaaa ttctcccatc tatctggaat
    13561 taagaaatca agtggtaaaa agaggaacag ggatactgat tctggattgc tataatgcta
    13621 atccttattc tatgcaaaaa gcattagaat attggcaaac actatcttca gaacaacctc
    13681 attatgctgt tttaggtgat atgctggaac ttggaaatag ctctgttatg tatcataaaa
    13741 tgattgctgc tatgctttct gaaatgcgct acgaaaaact gatcactgtt ggttcctttt
    13801 cgcgattgtt tcatcctgat agagaaaatg ctatcctgca ttataataat gtggaagaat
    13861 taattgcttc gggaaattta cgaacctttc ccaacaatgc aataattcta gttaagggct
    13921 cacattcaat tcaactggag cgcataatcc caatccttaa aggagagaac taatgtttta
    13981 tcatcttctt taccctcttg ctaaatatag catcgttttt aatgtgtttc gctatgtaac
    14041 cttccgttct attggagctt ttattacttc tttgataata tcgcttttgg tgggcccaac
    14101 ttttattaaa atgctgcgta aaaggtctgc cgtagaaaca attgatgaag acctccctga
    14161 aaaacatcgc ttaaaacaag gaactccaac tatgggagga atcattatct tattttcact
    14221 gctcatttct tctttgttat ggaatgtact aaccaatcca gctattttaa tgatgtattt
    14281 ggc
//
LOCUS       DAORHB010000013        14266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3307_length_14266_cov_5.856942, whole genome shotgun sequence.
ACCESSION   DAORHB010000013 DAORHB010000000
VERSION     DAORHB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14266
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3307_length_14266_cov_5.856942"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1380
                     /locus_tag="PKM80_00870"
     CDS             373..1380
                     /locus_tag="PKM80_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV92489.1"
                     /translation="MKRLALFFLILCTFSVIWANPIVENLISRFWFEENNLLTIELSA
                     FIGLAQIETVTYLDAEDSLTITVNPNTTNVQVIYIPQSCNTPAQGYFTIRVFGMQDEI
                     VRWGEGLENDLSPLNGTQCGIQLLYSNLIGDSIYGWAKDTSPSACGPNYCSARFTYRV
                     ECTNLSGLPVAGVSVYHSQCCGNGFDNPAISDVAGIATDELWPMKTHLWVQNPLTSET
                     VFSTHFFAEPSELKVFSVQLPISVEDSVLPESEGRLSIHPSVISYSTGGVLHIAYDEK
                     ILGEAELILYDLKGQKICKTKYDKKEVDWQLPKLANGVYFVRLTSKGRALGTAKLIVI
                     K"
     gene            1377..1949
                     /locus_tag="PKM80_00875"
     CDS             1377..1949
                     /locus_tag="PKM80_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92490.1"
                     /translation="MKYVFLSVLIIGLATLSALSFEAEDLNFFLNDNLWVMEGLYYFA
                     NYDTTAVSQLIYFPVLCDSVCSPAILEKIEIHEGTNCQITMLQQMDNGFSYLLFLPAK
                     ALCTVQIIYSQHLKGNYVKYILTTANKWGRPLPYAKYTLVKGKEVQITSLPFPVQEQE
                     ADKYVWEFYDFTPKKDWEVYFVPHREKSEE"
     gene            2133..3341
                     /locus_tag="PKM80_00880"
     CDS             2133..3341
                     /locus_tag="PKM80_00880"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNV92491.1"
                     /translation="MKILVLNCGSSSIKYQLIDMQTENLIAEGIVEKIGEDIALFTYK
                     STRFTKKKREMVIDNHEQGLQLILEALIDPVTGVLSGLDEIDAVGHRLVHAGEHYSDA
                     VVVTDDVIRVLQECVSLAPLHNPANLIGIEAVKVNMPNCPQCGVFDTAFHQTMPAMAY
                     LYPLPLEFYTNHKIRRYGFHGTSHKYVSLKAADYLKRPVEELKMITCHLGNGASITAI
                     KNGKSVDTSMGFTPLEGLMMGTRCGDIDPAIPIHLQQQLGFTVDEVNTILNKKSGMLG
                     LSHISNDMREIEDEILIKNNPKAIMAMDVYCYRIKKYIGSYFAALNGLDVLVFTGGIG
                     ENMSMLREQVCREMEAIGIKLNLEENSKRIDGIQVLSAEDSKVIVLKIPTNEELMIAL
                     ETESLLRQEK"
     gene            3338..6253
                     /locus_tag="PKM80_00885"
     CDS             3338..6253
                     /locus_tag="PKM80_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV92492.1"
                     /translation="MNKMNQSFYRMLFDKSPVSLWIEDFTGIQAELEKMKKRGISDLR
                     THLKLHPDQLDALVNKLVVVDVNNATLSLYKAKDKQELLNNLQLVFKGEVKEYLLESF
                     IAVDEGKEYFEGQGINYDLEGNKLYFKITWSIPESEQKDYETVIVAMQDMTKLTLTSN
                     ELEKREALFRCIFEQSSEGMLLLDNSGTIMMANETMGEHLGIPVNRIIGSYLWDVYEE
                     FGKKVTYETSYELSKEKVLRVISEHQDRPREAQFSFLDSCHKVYARKHTLMPISTKDV
                     TLYAFISTDLTPAYRSERITAILHKISHAVNTTESLDELFHIIHDSLATIIDATNFYI
                     ALYDGKNNMITFPYLVDEMEENASPVVADDSKSLTAQIINEARPLLLDADEIQDRVKI
                     IGAYGVECKNFLGIPLILSGNVIGALVVQSYTKTNLYDEEDQLLLESVSEQIAFALHK
                     KQTDDSIKILIQAIEQAGEGTIIFSPEGNIKYVNSIFEKIIGFRKIDLIDKPFEFLPF
                     ESSKRWNMKQSWMRDRSSQPWRGKVDLTRKDGSKATLDMIVKPVIDDEGRLSSIVASC
                     KDVTYEILQEEQMKRSQRLEALGRLTGGIAHDFNNILSAIVGYTELAADDLIPESDAA
                     MNLLEVLKSATRAKEMIKQLISFSRQEESKTEVIDLVDHVRESVRFLKSYMPRSIQIK
                     EDYKTDKSSVLAVPSQIHQIVINLGTNSMQALKRDDALIEISVENVSFRSSDMQAFPE
                     LEQSQYLKISVSDNGTGIEPSIIDHIFDPYFTTKGATDGTGLGLSIVHSIIQAHHGAI
                     RVKSEPGFGTTFDVYLPLYTSEQRREDSTEEIVDEIDYSGTECIMFVDDEPSLVSVFR
                     QGMMRLGYKVEGFTDPRKAEEFFSKNPDAVDIVVTDTTMPYINGVDLAARLMTIKPDI
                     PIIICTGFTTLISMEEARQRGIRDYIMKPFKIKDIAIRIKDILNSAGSDDAPGSIAE"
     gene            6228..6488
                     /locus_tag="PKM80_00890"
     CDS             6228..6488
                     /locus_tag="PKM80_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNV92493.1"
                     /translation="MRLDLLLNKLCLTKTRSIAKNACDKNLVLVNGKPAKASTIIKAG
                     DIIIFRLYNAEHEIRITQIPEGNVAKKDSTKYYELLRKEDLS"
     gene            6525..7502
                     /locus_tag="PKM80_00895"
     CDS             6525..7502
                     /locus_tag="PKM80_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92494.1"
                     /translation="MLFLQKQKGIAVTNERNKKLLKRFFILLSLLIAGYLYANYTLQL
                     KTNNLVIYAESPISVPLETMLKSLDDNIDSFQMNIGVYLDLLAPVYLIENNTSYQTLS
                     LGKAKIVEFSDAFYSGSEQRIYVRPLASIQENHRKVLMHEYIHWYLEQIYEQAPLWFH
                     EGMATHYAQQVGFEQFIYYLEQSFLGKKSDLFRLSYSYPEKKEDWSLFYLSSAMAVRY
                     MSEKKPKEWQDFWELAASYQRKKTKASFTDCFVRAYRTTLYDFHKEYSQYTKRLSYQY
                     LFWSFNALLAILLPIILFIAWRIRKKRMAALPDLPLPEDEIINEDETVD"
     gene            7517..8188
                     /locus_tag="PKM80_00900"
     CDS             7517..8188
                     /locus_tag="PKM80_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNV92495.1"
                     /translation="METIQTHPYKPFLPEEAKVLLLGSAPPYRFCTGYINDLKNKDID
                     YYYGSNSNLLWDILFLALKPESINCISELRFRQDERIKRTAYQVNFLQSFLAENKLAM
                     ADILLKFIRKDTSAEDYKLKVLKYQDILTILYSRPTLNKILCTSRNRVHYWLVDYLQQ
                     KEKLDLVKMDENQTKLILKSGVQREIKIDILPSPSARSVMRYDNKEDFLQKVSEIYKQ
                     RILNN"
     gene            complement(8622..10307)
                     /gene="ftcD"
                     /locus_tag="PKM80_00905"
     CDS             complement(8622..10307)
                     /gene="ftcD"
                     /locus_tag="PKM80_00905"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNV92496.1"
                     /translation="MKLMECVPNFSEGRDSSILNAIAAAIKSVKGVVLLDMDPGADTN
                     RTVFTIAGEPEAVVEAAFQAIKKAAELIDMSKHKGAHPRMGATDVCPFIPISEMTMEE
                     CVEYANKLGKRVGEELGIPVYLYEYAATKEEWKNLANIRSGEYEALSEKAKDPYWKPD
                     FGPHTFNAKSGATAIGAREFLIAYNINLNTRDKKKASEIAQIIRESGRAARDKNGKLM
                     KDENGNTIYIPGLFTHCKAVGWFIESYDRAQISINLTNYKITPPHLVLEKVRELASEM
                     GIQVTGSELVGLIPKAAILEAGKFYLSRMNESTGIPERMIMQTAIQSMGLAELAPFDI
                     DKKVIEYAISRKDRLVNLKVDAFCDLLSTDEPAPGGGSVAALCASMSGALSAMVANLT
                     IGKKGYENVAKEAMELAPIGQEIKEKSLEVIDKDTDAFFTMMDAMRLPKKTEEEIEFR
                     KTQIEKCTQAAINAPLETMHLAMSAAELALKIAKIGNANALSDAGVAALTANCASQAA
                     YYNILINLPGIADENYIHKIHAEAKDLLEKCNLLCDSVEKDVLAKLMGEKVER"
     gene            complement(10322..11575)
                     /locus_tag="PKM80_00910"
     CDS             complement(10322..11575)
                     /locus_tag="PKM80_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV92497.1"
                     /translation="MAVAQIVLILIVLLFFLVLSFFFSGLETGMLSIDQIKLEQEAKK
                     SKSKAVLLQFVRQPDKFLGTTLIGNNIANVIIASLSTYLANRLFAPVFDARYTSLVVG
                     IVVLIFGEMIPKTIFRDNAETLIPLFFPLLQFFYYLLKPLVMIVTWLNNFLRKLLKLE
                     EGYQYDYLTKDDLSFLLSQTAIDETNDHQIEMIEDALDFTELDARNVMVPRTDIVAIQ
                     DKTSLSEVANIARKEGFTRYPVYCNNIDDIIGILIIYDILKQDCSPDMTAGDIIHEPL
                     FAPENTDLDVLLKEMQTKQRSMAIIVDSFGGTAGIVTMEDILEEIVGDIEDEYDTDED
                     ENSVQQVGPKTWLVAADTEIDRLIDDYGIQLPEGDYETVAGLILDRLEKIPHQGQVVN
                     VPPYTIQVLQATSKQIIKVKIHKIN"
     gene            complement(11576..12643)
                     /gene="prfA"
                     /locus_tag="PKM80_00915"
     CDS             complement(11576..12643)
                     /gene="prfA"
                     /locus_tag="PKM80_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNV92498.1"
                     /translation="MLPLEKLQSLENELNEMQEQISDPALINDTKKYRDCLRRFKELK
                     EIDSAWKHYRKLEKHMEEVKELEKSENDPEMLNLIKDEENSLQNQLEQAELKLRDLLI
                     PSDPNDSKNAIVEIRAGTGGEEAALFAADLFRMYSHYAEMKGWKLEVISMNETELGGY
                     KEVVFQLSGKDVYSYMRFESGVHRVQRIPVTESSGRIHTSAVTVAVLPEAEDIDIDIQ
                     DSDLRIDVYRSTGHGGQSVNTTDSAVRITHLPTGIVVTCQDEKSQIKNKAKALKVLRS
                     RLLDAEISRQEQEIARARKAQVSTGDRSAKIRTYNFPQSRVTDHRINLTSYNLDSFLA
                     GNIEEFIQALRIAWRNEKVNT"
     gene            complement(12649..13623)
                     /locus_tag="PKM80_00920"
     CDS             complement(12649..13623)
                     /locus_tag="PKM80_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNV92499.1"
                     /translation="MSEKKEISVGGQAVIEGVMMRGPDLLATAIRRKDGSIELLKTPF
                     VSITQKKKFLGLPIIRGFISLIEMMIIGIKTLTFSANRFELDFIAEEKAKGKKVKSKN
                     KTAEQAENIVSYIIAFGLAFLLFGWLPYKLADWVHLSRQDFLFNVFAGVIRIIFFVLY
                     VYLISLMKDIKRLFRYHGAEHKNVNAYEHNSPLIIDKIKEWSTIHPRCGTSFIFFVLL
                     ISILIFSIVDTLVSAFILHSPVPVYLRLAYHILLMPLISGVSYEVLKFSGKNLKHPIV
                     KLLTVPGMALQHITTQPPDDEMLEIGLVAMKAALDMDISDHNVKIVEE"
     gene            complement(13704..13913)
                     /gene="rpmE"
                     /locus_tag="PKM80_00925"
     CDS             complement(13704..13913)
                     /gene="rpmE"
                     /locus_tag="PKM80_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNV92500.1"
                     /translation="MKKAIHPKYEMVTVTCACGNTFQTKSTKDKLQVEICNACHPFYT
                     GKQKIIDTAGRVEKFNKKYNLKQEN"
     gene            complement(14069..>14266)
                     /locus_tag="PKM80_00930"
     CDS             complement(14069..>14266)
                     /locus_tag="PKM80_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92501.1"
                     /translation="RCAATAALKIVRRKQNCRAEIQVRECKVQSAGVRSSKCGSAKFK
                     VILSVTSVISVANYKKSLKSL"
BASE COUNT         4456 a         2549 c         2746 g         4515 t
ORIGIN      
        1 aggttttaag gggcggaaat agtttttctt ataggagtgt ttattgctgg gtggaaacct
       61 gtcgcggata tctatcgttt ctacggaata agtactttat ccttgaaatc ctgtaatcgc
      121 gttcctaata tcaaattttc agatattcag atagtctttt ttattccagg caattaaatc
      181 tgcgtgatta ccgagagatt attatgttgc ataaaaggga cggtaatttg cctatttgta
      241 taataaaggg gacggtggct attttaattg tgcgctaaaa aggtatcttc tttccagcaa
      301 tgattataaa agcaggttct ggaataaaag ttgcttataa ttatggtaat ataaaaaagg
      361 gggagaagaa aaatgaaacg gctcgctcta ttttttttaa tcctttgcac cttttccgtt
      421 atttgggcaa atcccatagt ggaaaattta atcagcagat tctggtttga ggaaaataat
      481 ttactgacga ttgaattgag cgcttttata ggtttagctc aaattgaaac ggtaacttat
      541 cttgatgctg aggactctct tactataact gttaatccta atactacgaa tgttcaagta
      601 atttatattc cccaaagctg taatacacca gcgcaaggat attttactat acgagtgttt
      661 ggaatgcagg atgaaattgt tcgttgggga gagggtttgg aaaacgattt aagtccgcta
      721 aacggaactc aatgtggtat tcaattgctt tattctaatt taattggaga tagtatctat
      781 ggttgggcaa aagacacatc accttcagcc tgcggtccta attattgttc tgccagattt
      841 acttatcggg ttgaatgtac taatttaagt ggattgcctg ttgctggtgt ttctgtttat
      901 cacagtcagt gttgtggaaa tggatttgat aatccagcta tatctgatgt ggcaggtatt
      961 gctacagacg agttatggcc tatgaaaacg catctttggg tgcaaaatcc gctaactagt
     1021 gaaacggttt ttagcactca tttttttgct gagccatcgg aattaaaggt tttttcggtg
     1081 caattaccta tttctgtgga agatagtgtt ttgcctgaat ctgaggggag gctttctatt
     1141 catccttccg taatatctta tagtacggga ggagttttac atattgctta tgacgagaag
     1201 attttaggag aagcagagct gattttatat gacctcaagg ggcaaaaaat ttgtaagaca
     1261 aaatacgata aaaaggaagt ggattggcag cttcccaagc ttgctaatgg cgtatatttt
     1321 gtgcgtttaa cgagcaaagg cagagcattg ggaacagcta aactgattgt tatcaagtga
     1381 aatatgtttt tctgtcggtt ctaattatcg ggttagcaac cctttctgct ctcagttttg
     1441 aggcggaaga ccttaatttt ttcctaaatg ataacttatg ggtgatggag ggtttatatt
     1501 attttgctaa ttatgatacg acagctgttt cccaactaat ctattttccc gttttatgtg
     1561 atagtgtatg ttcacctgct atactagaaa aaatagagat tcacgaaggt acaaactgcc
     1621 agataactat gttgcagcaa atggacaacg ggttttcata tctgcttttc ctacctgcaa
     1681 aagctttatg cacggtgcaa attatttatt ctcagcacct gaaaggcaat tatgtaaaat
     1741 acattctcac tacggcaaat aaatggggaa ggccattacc ctatgcaaaa tatactttag
     1801 taaaggggaa agaggtgcaa ataacttctc ttccttttcc ggttcaggaa caggaagcag
     1861 ataaatatgt gtgggagttc tatgatttta ctcccaagaa agattgggaa gtgtattttg
     1921 tgcctcatag ggaaaaaagc gaggaataaa aaatgcctaa ttagctctgc tgttttttta
     1981 ttatcagagt tggaagattt tagcaacaga tagataatca aggagataaa aggataatta
     2041 cgataaaatg caaaaagaaa gaaaaaagga tttgacagtt attcaagaaa agaagaaaag
     2101 acacacaact aatttcaata agttgaggaa agatgaaaat attagtatta aattgtggta
     2161 gttcatccat taaatatcag ctaattgata tgcaaacgga gaacttaatt gcagagggca
     2221 tagtagaaaa aatcggggaa gacattgcac tttttactta taaaagcacc agatttacca
     2281 aaaagaagcg ggaaatggta attgataatc atgaacaagg tctgcagctt atactggaag
     2341 cattaattga tccggttact ggtgtcttga gtggtttaga tgaaattgat gcagtaggtc
     2401 atcgattggt acatgcagga gaacattatt ccgatgcggt tgttgttaca gatgatgtaa
     2461 ttagggtatt gcaagaatgc gtatctttgg ctccattaca taatcctgct aatttaattg
     2521 gcatagaagc agtgaaagtt aatatgccaa attgtcctca atgtggtgtt tttgatactg
     2581 cattccatca aacgatgcct gctatggcat atttatatcc tctaccttta gaattttaca
     2641 caaaccataa aatacggcgt tatggatttc atggaaccag ccacaaatat gtaagtctga
     2701 aggcggctga ttatttaaag agacctgtgg aagagctgaa aatgatcact tgccatttag
     2761 gtaacggagc atccataact gccataaaaa atggaaaatc agttgatact tctatgggtt
     2821 tcactccttt ggaaggacta atgatgggaa cccgttgtgg agatatcgat cctgctattc
     2881 cgattcattt gcaacagcag cttgggttta cagttgatga agttaatact attttaaaca
     2941 aaaaaagcgg aatgctgggc ttaagccata tttccaatga tatgcgcgaa attgaagatg
     3001 aaatattaat taagaataac ccaaaagcta ttatggctat ggatgtatat tgctatcgga
     3061 taaaaaaata tataggcagc tattttgcag ctttaaacgg attagatgtc ctggttttta
     3121 ctggcggaat aggtgaaaat atgtctatgt tacgagaaca ggtttgccgc gaaatggaag
     3181 caattggaat aaaattaaat ttggaagaaa attcaaaacg cattgatggt atccaggttt
     3241 tgagtgcaga ggattctaag gttattgtac ttaaaatacc taccaatgaa gagctgatga
     3301 ttgctctgga aactgaaagt ttattaaggc aggaaaaatg aacaaaatga atcagtcatt
     3361 ttaccggatg ctgtttgaca agtcaccggt atctttatgg atcgaagatt ttactggaat
     3421 ccaggcagaa ctggaaaaaa tgaaaaaaag gggtatttct gatcttagaa ctcatctgaa
     3481 attgcatcct gatcaactgg atgctttagt aaataaacta gtagttgtgg atgtaaacaa
     3541 cgcaactcta tctctttata aagctaaaga taagcaggag ctattgaata atctgcagct
     3601 agtatttaaa ggtgaagtaa aagaatattt actggagtct tttattgcgg ttgatgaagg
     3661 aaaagagtat tttgaagggc agggtataaa ttatgatctg gaaggcaaca aactgtattt
     3721 taaaattact tggagcattc ctgaaagtga acagaaagac tatgaaactg tgattgttgc
     3781 tatgcaggat atgacaaagt taactttaac cagtaatgaa ttggaaaaaa gagaagcgct
     3841 gtttcgttgt atttttgaac agtcctcgga aggaatgctg ttgcttgata attctggtac
     3901 gattatgatg gctaatgaaa caatgggaga acatcttggt atacccgtaa atagaataat
     3961 cggttcatat ctgtgggatg tttatgagga attcggcaag aaagttacat acgaaaccag
     4021 ttatgaattg agcaaagaaa aggtactaag agttatttcc gaacatcagg atagaccccg
     4081 agaagcacaa ttttcctttc tggatagctg ccataaagta tatgcccgca aacacacttt
     4141 gatgccaatt tctacaaagg atgtaactct gtatgctttt atttctacag atctcactcc
     4201 ggcttatcgt tctgaaagaa ttactgctat tcttcataaa atcagccatg cagttaatac
     4261 tactgagtca ttggatgaac tttttcatat tatccacgat tccttagcaa cgattattga
     4321 tgccacaaat ttctatattg ctctatacga tgggaaaaac aatatgatta ccttccctta
     4381 tctggttgat gaaatggaag aaaatgcttc tccggttgtg gctgatgaca gtaaatctct
     4441 aacggctcag ataattaatg aagccagacc tttgcttttg gatgctgacg aaattcagga
     4501 tagagttaaa ataattggag cttatggagt tgaatgcaaa aactttttgg ggattccttt
     4561 aatcctctcc gggaatgtga tcggggcgtt agtagttcag tcatacacaa aaacaaatct
     4621 ctatgatgaa gaagatcagt tgctgttgga atctgtttct gaacaaattg cctttgcttt
     4681 gcataagaag cagacagatg atagtattaa aatcctaatt caggcaattg aacaagccgg
     4741 agaaggcact ataatctttt ctccggaagg aaatattaaa tatgtaaatt ccatttttga
     4801 gaaaattatc ggttttcgta aaattgattt gatcgataaa cccttcgaat ttcttccttt
     4861 tgaatcgagt aaaagatgga atatgaaaca atcttggatg cgtgatcgtt cttcacagcc
     4921 ctggcgggga aaagttgatt taaccaggaa agatggctct aaagctactt tagatatgat
     4981 tgtgaaacct gttattgatg atgagggacg cctttccagt atagttgcca gctgtaaaga
     5041 tgttacttat gaaattttgc aagaagaaca aatgaaaaga tctcaacggt tggaagcgct
     5101 gggacgatta actggaggta ttgcacacga ttttaataat atcttaagtg caattgttgg
     5161 ttatacagaa ttggctgcag atgatcttat tcctgaaagc gatgccgcga tgaatttact
     5221 tgaggtttta aaatctgcca ccagagctaa ggaaatgatt aaacagctga tttctttctc
     5281 ccgtcaggaa gaatctaaaa cagaagtaat tgatttggtt gatcatgtaa gagaatcagt
     5341 ccgttttctg aagtcctata tgccgcgttc tatccaaata aaagaagatt ataaaactga
     5401 taaaagttct gtgcttgcgg ttccgagcca aatacatcaa atagtgatta atcttggtac
     5461 aaactctatg caagctttaa aaagagatga tgccttgatc gaaatatccg tagaaaatgt
     5521 ttcatttcgc agtagtgata tgcaggcatt tccggaactg gaacaaagcc aatatttgaa
     5581 aatatcggtt agtgataatg gtacgggaat agaaccttct atcatagacc acatatttga
     5641 tccctatttt actactaaag gtgctacaga tggaactggt ttggggcttt ccattgtaca
     5701 tagtattatt caggctcatc atggagctat cagagtgaaa agtgaacccg gatttggaac
     5761 tacttttgat gtgtatttac ctttatatac atctgaacag agacgggagg atagcactga
     5821 agaaattgta gacgaaattg actacagcgg aactgaatgt ataatgtttg tagatgatga
     5881 accgagctta gtttctgttt ttagacaagg gatgatgcgc ttaggttata aagtggaagg
     5941 atttaccgac ccgcgtaaag cagaagaatt ttttagtaag aatcctgatg ctgtagatat
     6001 tgttgttact gatactacga tgccttacat taatggtgtt gatcttgctg caagactgat
     6061 gactataaaa cccgatattc caataataat ctgcactggg tttaccaccc taatttctat
     6121 ggaagaagct cgacaacgcg gtattcgaga ttatattatg aaaccattca aaataaaaga
     6181 tatcgctatc cggattaagg atattttaaa ttctgcaggg agtgacgatg cgcctggatc
     6241 tattgctgaa taaactgtgc ctaactaaaa cccgttccat agctaaaaat gcctgcgata
     6301 aaaacctggt gttagttaat ggaaaaccag caaaggcatc cacgataatt aaagctggtg
     6361 atattattat ttttcggctt tataatgcgg aacacgaaat ccggataacg caaattccag
     6421 aaggaaatgt agcgaaaaag gacagcacga aatattacga gttgttacgc aaagaagacc
     6481 tttcctgatc aggggcatct agctatgtcc ttttttctgc ttttatgtta ttccttcaga
     6541 agcagaaagg aattgctgta acaaatgaaa ggaataagaa attgctaaag cgctttttta
     6601 tcttgctttc gttattgatt gcgggatacc tttatgcaaa ctacaccctg cagctaaaaa
     6661 ctaataatct tgttatatat gcagaaagtc caatttccgt tcctttggaa acaatgttga
     6721 agtccttaga tgataatatt gatagttttc agatgaatat aggagtatat cttgatctgc
     6781 ttgcgcctgt ctacctgatt gaaaataata caagttatca aactctttct ttagggaagg
     6841 caaaaatagt ggaatttagt gatgctttct atagtgggtc agaacaaaga atctatgtgc
     6901 gtcctcttgc ttcaattcaa gaaaatcatc gcaaggtttt gatgcatgaa tatattcatt
     6961 ggtatctgga gcaaatttat gaacaggcac ctttatggtt tcatgaaggg atggctacgc
     7021 attatgctca gcaggtaggt tttgaacagt ttatctatta tttggaacag agttttttgg
     7081 ggaaaaaaag tgatctgttt cggctcagtt atagctatcc agaaaaaaaa gaagattggt
     7141 ctttattcta tttatcttcg gcaatggctg ttcgctatat gagtgaaaaa aaaccaaagg
     7201 aatggcagga tttttgggaa ctggcagcga gctatcagag aaaaaaaact aaggcatctt
     7261 tcaccgattg ctttgtccgt gcttacagaa ctacactata tgattttcat aaggaatatt
     7321 ctcaatatac caagcggtta agttatcaat atctgttttg gagctttaat gctcttctgg
     7381 ctatactttt accgattatt ttatttatcg cctggcgaat caggaaaaaa agaatggcag
     7441 ctctccccga tttaccttta ccggaagatg aaattattaa cgaagacgag actgtagatt
     7501 aatcactttt tgatatatgg aaaccataca aacccatccc tataaacctt tcttgcctga
     7561 agaagctaaa gttttacttc tgggaagtgc tcctccctat cgtttttgca caggatatat
     7621 caatgatctt aaaaataagg atattgacta ctactatggc agcaacagca atttgctttg
     7681 ggatattttg tttttggctc taaaaccaga atcaataaac tgtatttcgg aattaagatt
     7741 taggcaagac gagagaataa aaagaactgc ttatcaggtt aattttttac aaagcttctt
     7801 agccgaaaat aagctggcaa tggcagatat cctcttgaaa tttatacgca aggataccag
     7861 cgcagaggat tataaactga aagttttaaa atatcaggat attttaacaa ttttgtatag
     7921 ccgtcccacc ctcaataaaa ttctctgcac cagcagaaac agagttcatt attggttagt
     7981 ggactacctc cagcaaaaag aaaaattgga tttagtgaaa atggacgaaa accaaactaa
     8041 gctaattcta aaaagcgggg ttcaaagaga aataaagatt gatattttgc cttctccttc
     8101 ggcaagaagt gttatgcgtt atgataataa agaggacttt ctgcaaaaag tatccgaaat
     8161 ctacaaacaa agaatattga acaattagcg ataaggacac aatctcgatg attttttaac
     8221 taaaagggag aagtattcga tcatattcgg tgtttttatc tggattttac ctttttttgc
     8281 tgtcctaaaa ccctcttttt cgttcatctt ttaactcttg aaaattgggc ttacctctcc
     8341 aacaagcaat tagaaaaagt taaaatagct gaactgtgta tcagagagag aaaaaataag
     8401 ggaaaagata gatttattag gttaggaaaa gtaatggagt atttatataa agatagagtc
     8461 cttaccaccg aaataagttt ccaacatacc tataactcgt ttggcagtga ggactcctta
     8521 taaagaaggt tattttccac aaattaaatc gtgggtttca cctctcgact tctcgacttc
     8581 tcgacctctc gacttctcga cttctcgact tcccgacttt ttcacctttc cactttttca
     8641 cccattaact tagccaaaac atctttttct acgctatcac aaagcaaatt acatttttcc
     8701 agcagatctt ttgcttcggc gtggatttta tgtatataat tttcatcagc aattccggga
     8761 agattaatca ggatattata gtaagcagct tgagaagcgc aatttgcagt taaagcagcc
     8821 actccagcat cggaaagagc attggcatta ccaattttag ctatttttaa ggcaagttcg
     8881 gcagcggaca ttgctaagtg catagtttcc aaaggagcat taattgcagc ttgagtgcat
     8941 ttttcaattt gagttttgcg aaattctatt tcttcttcgg tttttttggg taaacgcata
     9001 gcatccatca tagtgaagaa ggcatctgta tctttatcaa tcacttccag cgacttttct
     9061 tttatttcct gtccaatagg ggctagttcc atagcttctt tagccacatt ttcatatcct
     9121 tttttgccaa tagtaagatt tgcaaccatc gcagaaagag ctccactcat agatgcacaa
     9181 agagcagcaa cacttccacc tccgggagcg ggttcatcag tagaaagcaa atcacagaag
     9241 gcatccactt tcaggtttac caatcgatct tttctggaga tggcatattc aataactttt
     9301 ttatctatat cgaagggagc taattctgcc aagcccatag attgaattgc tgtttgcata
     9361 atcattcttt cggggatacc ggtactttcg ttcattctac ttaaataaaa ttttccagct
     9421 tctaaaatag ccgctttagg gattaagcca accagttcac tgccagtaac ttgaatgccc
     9481 atttcgctgg ccagttcgcg cactttttcc aaaaccagat ggggaggggt aattttataa
     9541 ttggtaagat ttatactaat ttgagcgcga tcgtaggatt caatgaacca gccaacagct
     9601 ttacagtgag taaataggcc agggatatag atggtatttc cgttttcatc tttcatcagt
     9661 ttaccatttt tatcccttgc tgctcttccg ctttcgcgaa ttatttgagc tatttcactt
     9721 gcttttttct tgtcgcgagt attgaggttt atattatagg caattagaaa ttctctcgct
     9781 ccaatagctg tagctccact tttggcatta aaagtatggg gaccgaaatc gggtttccaa
     9841 tatgggtctt tagctttttc agatagtgct tcatattccc cgctacggat attagccaga
     9901 tttttccatt cttctttagt ggctgcatat tcatataagt aaacaggtat gccaagttct
     9961 tcgccaactc gttttcctaa tttatttgca tattctacgc attcttccat cgtcatttcc
    10021 gaaattggga tgaagggaca tacatcggta gctcccattc tgggatgagc tcctttatgt
    10081 ttactcatat caatcagttc agcggctttt ttaattgcct gaaaagctgc ttccacaact
    10141 gcttcgggtt ctccagctat ggtaaaaaca gttctgtttg tatcagcgcc cggatccata
    10201 tccaataaaa ccactccctt aacactttta atagctgctg caatagcatt tagaatggag
    10261 ctgtctcttc cttcactgaa attggggaca cattccatca gtttcataat tgaacctcta
    10321 tctaatttat tttatgtatt ttaaccttta taatctgttt tgaagttgcc tgtaaaacct
    10381 gtatagtata aggaggaaca ttaactacct gaccctgatg cgggattttt tccaagcggt
    10441 ctaaaattaa acctgcaact gtttcataat cgccttcggg caattgaata ccgtaatcat
    10501 ctatcaatct atcaatttca gtatcagcgg caaccagcca tgtctttggt ccaacttgtt
    10561 gaacagagtt ttcatcttca tcggtatcat attcatcttc aatatcacca acgatctctt
    10621 ccaggatatc ttccatagta acaattccgg ctgttcctcc aaaggaatca acaataatag
    10681 ccatactgcg ttgtttagtt tgcatttctt tcaaaaggac atccagatct gtattttcag
    10741 gagcgaagag aggttcgtga ataatatctc ctgcagtcat atcaggagag caatcctgtt
    10801 tcagaatatc ataaataata agaattccga ttatatcatc aatgttgtta caatatacag
    10861 gatagcgagt aaaaccttct ttacgagcaa tattggctac ttcagataga gaggttttat
    10921 cttgaatggc aacaatgtct gtacgaggaa ccataacatt tctggcatcc agttctgtaa
    10981 aatccaaggc atcttcaatc atctcaattt ggtgatcgtt tgtttcatct atagcggttt
    11041 gggaaagcag aaaggagagg tcatcctttg ttaaataatc gtactgatat ccctcttcca
    11101 gtttcagcaa tttccgcaag aaattgttca gccaggtaac tatcattacc aacggtttca
    11161 gcagataata gaaaaattgc aatagaggaa aaaacaaagg gatcagcgtt tcggcattat
    11221 ctctgaaaat agttttggga atcatttctc caaatatcag cacaactatc cctactacca
    11281 aagaagtgta tcgagcatca aagacagggg caaagagtcg atttgctaaa taggtagaaa
    11341 gagaggcaat aatcacatta gcaatgttat taccaattaa agtggtacct aaaaatttat
    11401 cgggttgacg gacaaactgc aaaagaacag ctttgctttt actctttttg gcttcttgtt
    11461 ccaattttat ctggtctata gaaagcattc ccgtttccaa gccagaaaag aaaaaactaa
    11521 gcaccagaaa aaaaagtaat acgatcagga tcagaacaat ctgagccact gccatttatg
    11581 tattcacctt ctcgtttctc caggcaattc gtaaagcctg aataaactct tctatatttc
    11641 ctgccaaaaa ggaatctaga ttgtagcttg tcaagttaat tctatgatct gtaactcggg
    11701 attgtggaaa attataagtt cggatttttg cactgcggtc accggtggag acctgagctt
    11761 ttctagctct ggcaatttct tgttcctgac ggcttatctc agcatccagt aaacggctgc
    11821 gtaacacttt taaggcttta gctttgtttt taatctgcga tttttcgtcc tggcaggtaa
    11881 ccacaattcc tgtaggcaga tgagtaattc tcactgcgga atcagttgta ttaacacttt
    11941 gtccgccatg ccctgtactt ctatagacat ctatgcgcaa atcactatcc tggatatcta
    12001 tatctatatc ttctgcttca ggtaaaacag ccacagtaac agcagaagta tgaattctac
    12061 cagaggattc tgttaccgga attctttgca ctctatgtac cccgctttca aaacgcatat
    12121 aggaatatac atctttaccg ctaagctgaa aaacaacttc tttatagcca cccagttcgg
    12181 tttcgttcat tgagatcact tccagtttcc agcctttcat ttctgcataa tggctataca
    12241 ttctaaataa atcagcagca aaaagagcag cttcttctcc acctgttcct gcccgaattt
    12301 ccacaatggc atttttggaa tcattaggat cagagggaat aagcaaatcg cgcaatttta
    12361 gttctgcctg ttcaagttgg ttttgcaaag agttttcttc atctttaatt agatttagca
    12421 tttctggatc gttttcgctt ttttccagtt ccttcacttc ttccatatgt ttttccagtt
    12481 tccgataatg tttccaggca gaatcaattt cttttagttc tttaaagcgg cgcaagcaat
    12541 cacggtactt tttagtatca ttgatcaaag caggatcgct gatttgttcc tgcatttcat
    12601 tcagttcatt ttccaggctt tgtaattttt cgagaggtag caagagtgtt attcctccac
    12661 gatttttaca ttatgatcac ttatatccat atccagagca gccttcatag caactaaacc
    12721 gatttctagc atttcatcat caggtggctg agtagttata tgttgtaaag ccatcccagg
    12781 aacggttaaa agttttacga taggatgttt tagatttttt ccggagaatt tcagcacttc
    12841 ataagaaaca cccgaaatta atggcatcag taaaatatga tatgctaagc gtaaataaac
    12901 tggaactgga gaatgcaaaa taaaagctga aactaaagta tccacaatgg aaaagatcag
    12961 gatgctgatt aataacacaa aaaagataaa actcgttcca caacgaggat ggatagtaga
    13021 ccattcctta attttatcta ttattaaagg actgttatgt tcataggcat ttacattttt
    13081 atgctcagct ccatgatagc gaaacaacct ttttatatcc ttcatcaggg aaatcaaata
    13141 aacatagagt acgaagaata ttatcctgat tactccagca aagacattaa acagaaaatc
    13201 ctgccgcgaa agatgaaccc aatcagcaag tttatatggt aaccatccaa acaggagaaa
    13261 agctaagcca aaggcaatta tataactgac gatgttttcc gcttgttctg cagttttgtt
    13321 tttacttttc acttttttac ccttggcttt ctcttctgct ataaaatcca gctcaaaacg
    13381 attggcagaa aaagtgaggg tcttaattcc aataatcatc atttcaatta aggaaataaa
    13441 accacgaata atcggcagcc ccaaaaattt cttcttctga gtaatgctga caaagggcgt
    13501 ttttaacaat tcaatactgc catcttttcg gcgaatagca gttgccagaa gatctggacc
    13561 gcgcatcatt acaccctcga taacagcttg gccccctacg ctaatttctt tcttttcaga
    13621 catatttctt cctaagatag aaaaaacgcc attttccccg atttcagaaa acggataaaa
    13681 tggcgtaaac tgggtataaa accctaattt tcctgtttaa gattatattt tttattgaat
    13741 ttctctactc ttccagcagt atcaataatc ttctgcttac ctgtataaaa aggatgacat
    13801 gcattgcaga tttctacctg caatttatct ttagtgcttt tggtttgaaa tgtatttcca
    13861 caagcacagg taacggttac catttcgtat ttaggatgta ttgctttctt catctatctt
    13921 tttctccttg aaaatcttaa tcgtacttat accacaaatt tagatggcag aattatgtca
    13981 atgcaaaaag tggggaaatt tggattgtct ctcacagatt acagatttca cagattaaaa
    14041 atagataata ggtctgggat gaacaggatt aaagggattt tagggatttt ttataattag
    14101 ccaccgagat caccgaagtt accgaaagaa taactttgaa ctttgcactc ccgcactttg
    14161 aactacgaac tcccgcactt tgaactttgc actcccgcac ttgaatttcc gctctacaat
    14221 tctgtttcct ccttactatc ttcagtgctg ctgtggcagc gcacct
//
LOCUS       DAORHB010000014        14192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3336_length_14192_cov_6.070666, whole genome shotgun sequence.
ACCESSION   DAORHB010000014 DAORHB010000000
VERSION     DAORHB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3336_length_14192_cov_6.070666"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..450)
                     /locus_tag="PKM80_00935"
     CDS             complement(13..450)
                     /locus_tag="PKM80_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92502.1"
                     /translation="MKVKYRNALKSYQGKNDGMVYYYNSRLKKSICRRYAYPKITQNN
                     LHFAQINHNLKELNPSEGYKSDLKMYANIIKADKKEKGYICWNNVYRKLMFALAKDKD
                     LDLASLTREDIYSQNLPCKTVKESIEAGYLSPVEGYQTFVHEL"
     gene            1093..2424
                     /gene="dnaA"
                     /locus_tag="PKM80_00940"
     CDS             1093..2424
                     /gene="dnaA"
                     /locus_tag="PKM80_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNV92503.1"
                     /translation="MDQDIWQNILDKLEENINPQSFKTWFSDTRLVDMNDKELMIRVS
                     TQFSANYLNQNYKEILSEISYSLYGRKYDIQFITQPRHNHNYNSDYPEIKSNRVTSNV
                     QLNERYTFDEFVVGKNNNFAYSAAKAVAESPGYTYNPLFIYGESGMGKTHLMQAIGNF
                     VLKEGRNSSIYYITSEAFTNAMIDGIRSNKMPEFREKFRKVDLLLMDDIHFLSRKEGT
                     QEEFFHTFNALFDNKKQIVLTSDRPPKDIPDLEKRLVTRFESGLLCDLKNPDFETRVA
                     ILRKKAEPESVILADEIINFIADNITSSVRALEGSLIRILAFSSYNKINPEQIDVSMA
                     EDILSDMISEKTYSITLDAITQEVCKYYQITPAQILEKTRKPQVAFPRQVAMYLANYL
                     IPQLSLKEIAEYYNRKDHTTVIHAKKMIENQFREKEDFRIQLEMMIKNLQK"
     gene            2924..4108
                     /locus_tag="PKM80_00945"
     CDS             2924..4108
                     /locus_tag="PKM80_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92504.1"
                     /translation="MLVLIMISGCTDKKNLTGTNFSNVNAKSVNDKEGIIMGYSYPAE
                     SQTKISGSETKLLAGNYQNAVALSFLRFTELPSTIESVVSDSCFLTINIIKRSMLPRN
                     PLKLQLFKLNKIWNDSLDLSNLSAGDLSASPLAEYTITDTIGISSKEITFPLSVSDVF
                     SWSEPDSLGWNFVIKAENEGWVEIASLEGTNKPKLNIKYRKDSESAYLNYSKTPAKDT
                     YTLNAPETTACADWKISNLNSSRLYLKYDPTYTIFRDNEDNILDDTQIKRMTVNKAEV
                     VLHIKNNDYYNSYTSYSLYSFNVIRDSINSPVPLVTGDYEIIAYTVTSTGIIKGDSLT
                     VDVTPIIQAYTSGDKTPKGIMIQSLQERKNFGSLEFWDCNEGTLPEKKPYIKITYTPP
                     FL"
     gene            4111..4911
                     /locus_tag="PKM80_00950"
     CDS             4111..4911
                     /locus_tag="PKM80_00950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV92505.1"
                     /translation="MKKYIIIFVASILLFSCAKKEEKGIALAQVDKEVLYLEDFKSTF
                     SLEDWDNLSPEQRKKYIEDWVNLTLLSQYADSKGLDKDKAVKQKISYATKKVKANALI
                     AKRLSQIEVSEDQLFNYFRLHQGEFKKNAVEYNIQRIALVDKISAENVLQQLNQGMNF
                     AEAVQRYSTEELKYRGGYMGMVSSNGPDSLFWLKAREIKENEYGLVYKEPTWYVFRVL
                     ETKETLQEANFDDYRAEIKRKYLLEKQDQVYQDLIKEIKSQTSEVYYY"
     gene            5028..6284
                     /locus_tag="PKM80_00955"
     CDS             5028..6284
                     /locus_tag="PKM80_00955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV92506.1"
                     /translation="MRKLTITLILIIFSLMLSAELVDKIVAKVGTDIILLSDLQKELA
                     QMQRAGYLDENTDPRMVLEEMVNQKLIIQKAKDLKLTVNEDEIKSMAENYLKKIKAQY
                     PSPAAFSTDLKNSKLTESDLLQFYKDMLTEQSLIDQILKREITSKVSVSEAEVEHFYN
                     ETKDSLAVKPVSWELGLIVREIKPSEATKQAKLAEIKEIQNRLKNGEDFATIAKNESD
                     CPSKEVGGDLGFVKKGQMVKPFEDAAFALKLGEISDIVESNYGYHIIKLEEKKGEEIR
                     VRHILKMLSSTAEDSLRERQLMEEIRTRYANGESFASLAKEYSMDKESSEDGGSLGEF
                     TEKDIPPLFSVQIMQTPVGEMTPVLENSGLLYIFCRLQEYPPRMYSFNEVKDQVRDLV
                     LRMKQNEAYNSWIANLRKEAYVQITL"
     gene            6284..6778
                     /locus_tag="PKM80_00960"
     CDS             6284..6778
                     /locus_tag="PKM80_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNV92507.1"
                     /translation="MTEKQKKLNIYTFLAAFLGIGFIPFMPGTFGSLAAYGIYLLPKG
                     ELFIGTGLLYFIPAWLIFCLLAVYLSGKAEKTLGKDNGAIVIDEVCGYFVSVLFLPHN
                     WLIGLYAFALFRVFDIAKPFPIMRAQKIPAGWGIVIDDIIAGIYANLLIQILIKIYPR
                     FFGL"
     gene            6787..7140
                     /gene="yajC"
                     /locus_tag="PKM80_00965"
     CDS             6787..7140
                     /gene="yajC"
                     /locus_tag="PKM80_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNV92508.1"
                     /translation="MNYILIQATGKAATQPQQSGTGTTLIFMIIMFVILYLLLIRPQQ
                     KKAKETQKMQDSLKVNDKVLTSAGIYGRIVSLKPDKGIVVLEIDDNNKVRIDIQKNAI
                     VGVLNPSEPTPKNEE"
     gene            7130..7693
                     /gene="def"
                     /locus_tag="PKM80_00970"
     CDS             7130..7693
                     /gene="def"
                     /locus_tag="PKM80_00970"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNV92509.1"
                     /translation="MKNRPNLLPVRLYGDDLLRKKLPELDFNTEGLQEFIDDLIYTMY
                     KRDGVGLAANQVGSEWRLFVIDPDWANSENDNKNPIVMINPVIESRSGEIVYEEGCIS
                     LPDIFGSVKRSANISYSYTDRYGKRITENAEQTKAVVIQHEYDHLEGILFVDHLGTLA
                     RLKIMHQLKRLQARAKDGQNILEGLSL"
     gene            7693..8637
                     /gene="fmt"
                     /locus_tag="PKM80_00975"
     CDS             7693..8637
                     /gene="fmt"
                     /locus_tag="PKM80_00975"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNV92510.1"
                     /translation="MRYLFIGSSEYGLPALNKLIQNGYKPSLIISQPAKPAGRNRHII
                     PTPISEFADKQNLPLWTPENINSEEAIAKIAKYEAEIIITAAYGGYFKKNIRCLYPLG
                     TVNLHPSLLPKYRGASPIQSAILNGEKETGTTLYKVTAKMDAGPILLQKKLPILDNET
                     YSELNERLKQQAADLLWDFWKMLLKKQELMPTFQDESQASYCSKIDNNTCRINWQKTA
                     KEIHNQIRAFSLTPGAWTYFRSKKLKILASEKTKENTEGEPGLICGWDKNRGFFVNCL
                     DYKLLITKVQAAGKKVLSASAFANGARLAAKEKLGSDQ"
     gene            8634..9383
                     /locus_tag="PKM80_00980"
     CDS             8634..9383
                     /locus_tag="PKM80_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HNV92511.1"
                     /translation="MKKEYFAIIKFPMFVGLSLLAILLIFFLMVLGSYHHLGLSGWGN
                     FFYTLIFILIIILIASWILTLISTHCKIKSTWLRKYISWMLVRVYYPLAIWISIPFFQ
                     KRASIQESFLYFNNEIIVTNYQGIHFSNLLLLLPHCLQNSNCKIRISSDIVDCQECGG
                     CDIAKMKSITRDNHIKAAVASGGSLARKLVNDTKPDMIIAVACHRDLTEGVRDSWRYP
                     VYSVLNERPKGPCFETTVSYSVIDFAIKKFI"
     gene            9396..10232
                     /locus_tag="PKM80_00985"
     CDS             9396..10232
                     /locus_tag="PKM80_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV92512.1"
                     /translation="MKRINLFTVILILLLNAFITMALINKYMDKSVAIDNSYTEYSSK
                     PSKASAANRINSITEAVKAVEPAVVSINVIKTEYVRARSPFGFGFFDFFGFAPMMRQV
                     ESIGSGVIYDPKGYIITNAHVVSGATQIKVILPDKREYEGVIAGIDEIHDVAKLKING
                     NNLPVARLGNSNDLIIGEWSIAVGNPYGFLMNDSKPSVSVGVISAVSRNFAARTDKRE
                     YKSMIQTDAAVNPGNSGGPLVNINGEVVGINTFIFSESGGNIGIGFALPINSVKTILA
                     KI"
     gene            10253..12952
                     /locus_tag="PKM80_00990"
     CDS             10253..12952
                     /locus_tag="PKM80_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV92513.1"
                     /translation="MLIFIAGSLLCAQITHSCYDIQYNPEGGSNSPYLDQVVTVQGIV
                     SGISFYSGTSVNSYGFFIADAAGGPWSGLYIYNQTYHPNLGDLVQITGTVNEYYGLTE
                     ISQLTTFQVLSQDNPLPEPAEINSGALNNLSSGEQWESVLVKVLNVSVVTEPNNYQEF
                     YVDDGSGVCQIDDQFFGSGHSWSNITLGQVYSEITGIVDYAYNYYAINPRFLSDLVLS
                     GTTATLLIPHQAVSLHSSVTVPVNALGIDLASSYQSYSFNLAFNPNIISYQSVSTEGT
                     ISAPGTINASCTNGLLSVSYQSNSVISGQGVLLNLNFYATNTGTTVLALENVFFGNDS
                     IQSLIDGSLTVNSCYNSPGDTLTVIQRPLLNIPEIVIPGETLTITCLAPQNTTNWNAW
                     LKHSNKRINMPISNSQWLNNPNRWELVTTIPSVPIFELYDLEVSASGGISDITENAIQ
                     VIPTRKNNYYFVHITDAHLPNRLYYPNAGFETDSTAVEDFRAVMEDINLIHPEFVLFT
                     GDLVNEGELESFANQYWYGWTQKLLSEFDIPVYVTSGNHDIGGWNQTPPPAGSARRNW
                     WKYFGWNWLDSEDPNWLYHTQDYYFTYGNTIFIGLESYDNYDYWRLNIYGTTSFTDQQ
                     IAWLNSTLSLFPNYTKVLFHHYDFQDELNLSALGIDLALWGHIHSNNGSISNFPYNLA
                     TRSICDGNRAYRMVRVNNNQIIPYNTIYAGATGNNLSIQYFPSNYGVADSIRATIINN
                     QPLTFENAQIKFIMPSGMSGYRVQGGILEQVDRSGVNNVCYVRVSLSANSTTNISIAD
                     SGVENIDPLQVPKPLQIKSIYPNPLSLQGNIQLYSEKSLPTLTLQLYNLKGQLAYQQT
                     LQNINKGENTLAFTLPDSLGNGIYFLRLPEDKNTTQKILILKK"
     gene            12960..13967
                     /locus_tag="PKM80_00995"
     CDS             12960..13967
                     /locus_tag="PKM80_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV92514.1"
                     /translation="MPEKRPQTAKLLYIPWILKHLSYYVKLNTHILKKGVPLKEILIL
                     KFPFWFSDAKIPPLLSVDITDACNLKCNYCNNPFFPHPRTMMSKEVFEALIEHLKRQN
                     VSRIRISGGEPTLHPQFDTMLKEMSHYCKYLSIITNGQWKNKEITEKLLDCGLSFIEL
                     SMDAGGKEIYEKNRPGADYDLFLQNLKDLYELRNRKNTDLIIRIRLMLRPSTLSLEKE
                     DSHYWKQFSDCVLPQHILQHPETPPQEDVFMQDSILQNQLPVCTVTYRDLQIRPDGKV
                     PLCPAKGCAINLEDRIFIGDIQKDDILDLWNAPMLKQMRNAHRKRQGEILNLCRNCHY
                     G"
BASE COUNT         4761 a         2470 c         2706 g         4255 t
ORIGIN      
        1 aaatccttgt ttctataact cgtggacaaa ggtttggtat ccctcaactg gtgatagata
       61 accggcttca atactctctt ttactgtttt acagggcagg ttttggctat aaatatcctc
      121 gcgggtaagg gatgccagat ccagatcttt atctttagcc agagcgaaca ttagttttcg
      181 gtaaacatta ttccagcaga tatatccttt ttcctttttg tctgctttaa taatattcgc
      241 atacattttc aagtcggatt tatatccttc cgagggatta agttctttta ggttatggtt
      301 aatttgggca aaatgcaaat tattttgggt aattttgggg taagcatatc ggcggcatat
      361 tgatttttta agacgggaat tgtaataata caccattcca tcgttcttac cctgataact
      421 tttcagtgcg ttacggtatt taactttcat tttttctctc cttttaattt tgtcctctat
      481 atatataggg ggtgacaaat ctggccaagt ggtgacaaaa ttgtaacttt ttattttcct
      541 tagggtagct cctcatattc ctgacttctt ctcctccaaa acgaggggaa gaggaggagt
      601 catagacggg tagaggggga gaggagaagg ggagtttagc tggcaaaagc ttatgggaaa
      661 agaaataaaa tttttatgat atcggtaagt ctccctacct atagaattta cagctttata
      721 tcgcaaatct ccaaaatagc gatatatagc tataaagtta aaaatagcag ggaattatta
      781 attttactat ttgctaactt ctatatttgt tgaagtttag ctacttgaca aatcagagaa
      841 tagatttttt ttgggcatag ctgtcttttg tctcaggaaa agaattggat aagttcccat
      901 agaattgagt gttgctctac atttgtgctt aagctgtgga taacttgtgg ataactcagg
      961 gaaaggcagc gtcagccgct attagtttac ataattaagt ggttgacaat taacaagata
     1021 tctataataa acttgtttcc tgttttgcgg gaagcagttt gtggataaat aaattttaca
     1081 ggatattgaa ttatggatca ggatatttgg caaaacatac ttgataaact tgaagagaat
     1141 attaatcctc aaagtttcaa gacatggttt tcggatacaa gattagtaga tatgaacgac
     1201 aaggagctga tgataagggt ttctacccag ttttcggcga attatttgaa tcagaattac
     1261 aaggagattt taagcgaaat atcgtatagc ttatatggta ggaaatacga tattcagttt
     1321 atcactcaac ctcgtcataa tcacaattat aattctgatt atccagagat taaaagcaac
     1381 cgggttactt ccaatgtgca gttgaatgag cgttatactt ttgatgaatt tgtggtaggt
     1441 aagaataata attttgctta cagtgcggct aaggcagttg cagaatcacc tggttatact
     1501 tacaatcctc tttttatcta tggtgaaagc ggaatgggta aaacgcattt aatgcaagcc
     1561 attggcaatt ttgttttgaa agaagggcgt aatagttcaa tttattatat tacttccgag
     1621 gcgtttacca atgcaatgat tgatggaatt agaagcaata agatgccgga attcagggag
     1681 aaatttagaa aagttgatct gttattgatg gatgatattc attttttatc gcgcaaggaa
     1741 ggcacgcagg aggaattttt ccacactttt aacgctcttt ttgataataa gaagcagatt
     1801 gtgctcactt cggataggcc tcctaaagac atcccggatc tggaaaagcg gttggtaaca
     1861 agatttgaaa gcggtttact ctgcgacctg aaaaatcctg attttgagac gcgcgtagct
     1921 attctacgca aaaaagccga accggaaagt gtgattcttg ctgatgagat catcaatttt
     1981 attgctgata atataaccag tagtgtgcgt gctttggaag gatctttaat tcgcattctg
     2041 gctttctctt cttataacaa gataaatcct gagcagatag atgtttctat ggcggaagac
     2101 attctctctg atatgatttc ggaaaagaca tattcgatta ctttagatgc tatcacgcaa
     2161 gaggtttgta aatattatca aataacccct gcccaaatat tggaaaaaac aagaaagcct
     2221 caagttgctt ttccgcgtca ggtggctatg tatctggcaa attatttaat tccccagcta
     2281 tcattaaaag aaatagcgga atattataac cgtaaagatc atacaactgt gattcacgct
     2341 aagaagatga ttgaaaacca gtttcgcgag aaagaagatt ttcgaataca actggagatg
     2401 atgattaaaa atttacagaa gtaagcggtg gatatttgga atatccagag gaaaatttaa
     2461 agccggttga ttgataattt acagcttata ttgaaaataa tagaccaaaa accatataag
     2521 ctgagttaat atttttcttg attttaagga ttaaacaaag cttccttgaa atcgttataa
     2581 agatgtttat atatgtgaaa acaggctaaa aaagaagtga ctttaaatag ttatccacat
     2641 ttgtggatag gaaactgtgg atagttgtga ataacttgtg aataaaagag ttttgagttt
     2701 atgagtgtgg ataagctgtg tagaaaaagc cagtttgtcc acaagttatc cacattaaga
     2761 atagcgatcg atatttatat aagacagcga tcaaagaaaa agcttgacca aatatcccca
     2821 tattcacaac gcctacttat attactttat attagattta tattaaaata ggattatatt
     2881 tatggaatca ttcagaacat tttcaaaggc attttgctgc cttatgctcg tcttaattat
     2941 gataagcgga tgcacagata aaaaaaacct aacaggaaca aattttagta atgttaatgc
     3001 gaaatccgtt aatgataaag aaggaattat tatgggatat agttatcctg cagaatccca
     3061 aactaaaata tccggcagtg aaacaaaact gttggcagga aactatcaaa atgctgttgc
     3121 tcttagtttt ttacgattta cagagcttcc ttctacgata gagagtgtgg tttcagatag
     3181 ttgtttttta acaattaata ttataaaacg ctctatgcta ccgcgtaatc ctttaaaact
     3241 tcagcttttt aaattgaata aaatttggaa tgattctttg gatcttagca atctttcggc
     3301 aggagattta tcggcgagtc ctttagcaga atatacaata actgatacaa ttggcatttc
     3361 cagcaaggaa ataacttttc cgctttctgt ttccgatgtt ttttcttgga gcgaaccgga
     3421 tagtttaggt tggaattttg taattaaagc agaaaacgag ggttgggtag agatagcttc
     3481 tttggaagga accaataagc ccaagctaaa cataaaatat agaaaagatt cggaaagtgc
     3541 ttacttaaat tatagtaaaa ctcctgcaaa agatacctat actttaaatg caccagaaac
     3601 aacagcctgt gctgattgga aaataagcaa cctaaattct agtcgtcttt atttaaaata
     3661 tgatcctact tatactattt ttagggataa cgaagataat atattagatg acacccaaat
     3721 taagaggatg acggttaaca aagcagaagt agtgctccac ataaaaaata atgactatta
     3781 taattcttac acttcatatt cgctttattc tttcaatgta attagagatt ctataaattc
     3841 tcctgtgcca ttagttacag gtgattatga aataattgct tatacagtta ccagtactgg
     3901 aataattaaa ggagattcgc taacggttga tgtaactcca ataatacaag catacacttc
     3961 tggagataaa actcctaaag ggataatgat tcaatctttg caggagcgga agaattttgg
     4021 aagtttggaa ttttgggatt gcaacgaagg cacattgcca gagaaaaaac cttatataaa
     4081 aataacctat acaccaccct tcttatgaat atgaaaaaat acataattat atttgtcgcc
     4141 tctatattgc tgttttcttg tgcaaaaaaa gaagaaaaag gtatagcgct tgctcaagta
     4201 gataaagaag tgctatatct ggaagatttt aaatctactt tttctttgga agattgggat
     4261 aatctaagcc ctgaacagag gaaaaagtat attgaggact gggtaaatct tacgctttta
     4321 tctcaatacg cagatagtaa agggttggat aaagataaag ctgttaaaca aaaaatcagc
     4381 tatgccacaa aaaaagttaa ggcaaatgcg ttgattgcca aacgcctttc tcagatagag
     4441 gtttcggaag atcaattgtt taattacttt cgtttacatc aaggcgaatt taaaaaaaat
     4501 gcagtagaat ataacattca gcgtatagct ttagtagaca aaatatcagc ggaaaatgta
     4561 ttacagcagt taaaccaagg aatgaatttt gcggaagcgg tgcaacgata ttctacagaa
     4621 gaacttaaat acagaggtgg ttatatgggt atggtttctt ctaatggtcc ggattccctt
     4681 ttctggttaa aagccagaga aataaaagaa aatgaatatg gcttagtata taaagaacct
     4741 acttggtatg tatttcgcgt tttagaaact aaagaaactc ttcaggaagc aaattttgat
     4801 gattaccggg cagaaattaa acgcaagtat ctattggaaa aacaagacca ggtttatcag
     4861 gatttaatta aagaaattaa atcccaaacc agtgaagtat attattactg aaatgtttaa
     4921 attaaccaga tcaataagat tgaattatta tttagcaaac aatagagtgg cagatataga
     4981 atctgcctca aaacaaaacc tttacaaata aaaaaggaaa tattagaatg cgaaaactta
     5041 ctataactct tatcctgatt atcttttcct taatgttatc tgcggaatta gtagataaga
     5101 tagtagccaa agtaggaacg gatattattc tgctgtcaga cctccaaaaa gaactggcac
     5161 aaatgcaaag agcaggatat ttagatgaaa atacagaccc cagaatggtt ctggaagaaa
     5221 tggttaatca gaaattaatc atacaaaaag ctaaggactt aaagctaacc gtaaatgaag
     5281 atgaaataaa atcaatggct gaaaactacc taaaaaagat aaaagcccaa tatcctagtc
     5341 cagcagcttt ttctacagac cttaaaaata gcaaacttac ggaatctgac ctgttacaat
     5401 tctacaaaga tatgctaacc gaacaatctc tcatcgacca gatattgaaa agagaaatta
     5461 caagtaaggt atctgttagc gaagcagaag tggaacattt ttataatgaa acaaaggatt
     5521 ctttagcagt taaacctgtt tcctgggaac tggggcttat cgtaagagag ataaaaccaa
     5581 gcgaagcaac aaaacaagca aaactggcag aaattaaaga aatccaaaac agattaaaaa
     5641 atggggagga ttttgccaca atcgctaaaa acgaaagtga ttgccccagc aaagaagttg
     5701 gtggcgattt aggttttgtg aaaaaaggac agatggttaa accttttgag gatgcagcgt
     5761 ttgctttaaa acttggagag ataagtgaca tcgtagaaag taactatgga tatcatatca
     5821 tcaaattgga agaaaaaaaa ggcgaggaaa tccgcgttcg acatatctta aaaatgctat
     5881 cttccactgc agaagattca ttaagagaaa gacagctaat ggaagaaatc agaacccgct
     5941 atgctaatgg tgagagtttt gcttccttag caaaggaata ttctatggat aaagaaagca
     6001 gcgaagatgg aggttctttg ggtgaattta cagagaagga catacctcct cttttctccg
     6061 tgcaaattat gcaaacacct gttggagaaa tgactcccgt tttagaaaat tcaggtttat
     6121 tgtatatttt ctgtcgatta caagaatacc ctcccagaat gtatagcttt aatgaagtga
     6181 aagaccaggt gcgtgatttg gtattaagaa tgaaacaaaa tgaagcgtat aattcctgga
     6241 tagccaattt aagaaaagaa gcttatgtgc agattacact ataatgactg aaaaacaaaa
     6301 gaaactgaat atctatacct ttttagccgc cttcctcgga attggtttta ttccctttat
     6361 gccaggaact tttggttcgc tggctgctta tggaatttat cttttgccga agggagaact
     6421 ttttatagga acagggttat tatatttcat cccagcctgg ctgatatttt gtctacttgc
     6481 cgtttatcta agtggcaaag cagaaaaaac cttaggtaaa gataatgggg caattgttat
     6541 agatgaggtt tgcggctatt ttgttagtgt tctattttta cctcacaatt ggctgatagg
     6601 tctttatgcc tttgcgctgt ttagagtttt tgatattgcc aaaccctttc caattatgcg
     6661 agcacaaaaa atacccgctg gctggggaat tgttattgac gatattatag ccggaattta
     6721 tgctaacctc ttaattcaaa tactaattaa aatatatcca agattctttg gattatagga
     6781 gataaaatga actacatact aatacaagct acaggaaaag ctgccacgca accgcaacaa
     6841 tcaggaacag gaactaccct gatctttatg atcattatgt tcgtgattct gtatttattg
     6901 cttatccgtc cccaacaaaa gaaagctaaa gaaactcaga aaatgcaaga ttccctgaag
     6961 gttaacgata aagttttaac cagcgcagga atctatggac gcattgtatc gttaaaaccc
     7021 gataaaggaa ttgtggtttt agaaatagac gataataaca aagtgcgtat cgatattcaa
     7081 aaaaatgcaa ttgttggagt tttgaatcct tccgaaccta ccccaaaaaa tgaagaatag
     7141 accaaatctt ttacccgttc gcctctacgg agacgattta ttaagaaaga aacttcccga
     7201 gctggatttt aatacagaag gcttgcagga attcattgat gacctaattt atactatgta
     7261 caaaagagat ggcgttggtt tagctgctaa tcaagttggt tcggaatgga ggttatttgt
     7321 tatagatccg gattgggcta atagcgaaaa cgataacaaa aatccgatag tgatgattaa
     7381 tccagtaatc gaaagtcgct ctggagaaat tgtttatgaa gagggctgta tcagtttgcc
     7441 agatattttt ggaagcgtaa aacgcagtgc aaatatatct tattcatata cggatagata
     7501 tggtaagcgg attacagaaa acgctgaaca aacaaaggcg gttgttattc agcacgaata
     7561 tgatcatctg gaaggtatcc tgtttgtaga tcacctggga acccttgccc gcttgaaaat
     7621 tatgcaccaa ttaaaaagat tgcaggcaag agcaaaggac ggacagaata tattggaagg
     7681 actatcccta taatgcgtta cctatttatt ggctcttcag aatatgggtt acctgcccta
     7741 aataaactaa tccaaaacgg ctataaaccc tctctgataa taagccaacc agctaaacct
     7801 gctggacgca ataggcatat aataccaaca cctatttctg aatttgcaga taaacaaaat
     7861 ttgccactat ggacacctga gaacataaat tcagaggaag ccattgcgaa aatagcaaaa
     7921 tatgaagcag agattattat tacggcagct tatggcggat attttaaaaa aaatatccgc
     7981 tgcctctatc ctttgggaac ggttaatctc catccttctc ttttaccaaa atatagagga
     8041 gccagcccta ttcaaagtgc gatcttaaat ggtgaaaagg aaaccggaac tacgctatat
     8101 aaagtaacag caaaaatgga tgcaggtcct atcctacttc aaaaaaaact accaatttta
     8161 gataacgaaa cttacagcga actgaatgaa cgcttaaaac aacaagcagc agatttactt
     8221 tgggatttct ggaaaatgct gctaaaaaaa caagaattaa tgccaacctt tcaagatgaa
     8281 agccaagcct cctactgcag taaaattgac aataatacct gtaggataaa ctggcaaaaa
     8341 acggcaaaag aaatccataa ccaaatccgt gctttttctt taactcccgg cgcctggacc
     8401 tatttcagaa gcaaaaaact gaaaatattg gcttcagaaa aaactaagga aaacactgaa
     8461 ggagaaccgg gattaatttg cggatgggat aaaaaccgcg gtttttttgt taactgtctg
     8521 gattataaat tgctcattac taaagttcag gcagcaggca aaaaggtttt aagtgcttcc
     8581 gcttttgcta atggagcacg ccttgccgct aaagaaaaat taggaagtga ccaatgaaaa
     8641 aagaatattt tgccataatt aaattcccta tgtttgttgg cttatctctg cttgccatct
     8701 tgctgatttt ctttctgatg gttttgggca gctatcatca tttagggctt tcaggatggg
     8761 gaaatttctt ctataccctt atctttatat taattattat attaattgcc agctggattt
     8821 taaccctgat cagtacgcac tgtaaaataa aatcaacctg gctgagaaaa tatatttcct
     8881 ggatgctagt gcgtgtatac tatcctctgg ctatctggat ttctattccc ttcttccaga
     8941 aaagagcttc aatccaggaa tcttttctct attttaataa cgaaattatc gtaactaact
     9001 atcaagggat acacttttct aatctattat tgcttttacc acattgctta caaaattcta
     9061 actgtaaaat ccgcattagt agcgatatcg tagattgtca ggaatgcggt ggttgcgata
     9121 ttgccaaaat gaaatctatc accagagaca atcatataaa agctgccgta gcttctggtg
     9181 gttctttagc cagaaaattg gttaacgata caaaaccaga tatgattatt gcagttgcct
     9241 gtcatcgaga tttaacagaa ggagtgcgtg atagctggcg ttatcctgtt tactcagttt
     9301 taaatgaacg ccccaaagga ccctgtttcg aaacaaccgt tagctattca gtgatcgatt
     9361 ttgccataaa gaaattcata taaaaggaga aaaccttgaa acgcatcaat cttttcaccg
     9421 ttattctgat cctgttgcta aatgccttta tcacgatggc tttaattaat aaatatatgg
     9481 ataaatctgt tgcaattgat aattcataca cagaatattc ttccaaacca agtaaagcgt
     9541 cagctgccaa tcgcattaat tctataaccg aagctgttaa agcagtagaa ccagcagtag
     9601 ttagtatcaa tgtaataaaa acggaatatg tgcgagctag aagccccttc ggctttggtt
     9661 tttttgattt ctttggtttt gcaccaatga tgcgtcaagt ggaatctatc ggaagtggcg
     9721 ttatttacga tcctaagggc tatataatta caaatgccca tgtggttagt ggtgctactc
     9781 aaattaaagt tattttgccc gataaacgcg aatatgaagg tgtgatagca ggaatagatg
     9841 aaattcacga tgtggcaaaa cttaaaatta acggaaataa ccttcccgtt gccagattag
     9901 gaaattccaa cgatctaatt atcggagaat ggagtattgc tgttggtaat ccctatggct
     9961 ttttaatgaa cgattccaaa cccagtgtat cggttggagt tatttctgcc gtaagcagaa
    10021 attttgctgc tcggacagat aaacgcgaat ataaatctat gattcaaaca gacgctgctg
    10081 ttaatcctgg aaatagtggt gggccgctgg tgaatattaa tggcgaagtt gttggtataa
    10141 atacttttat cttttccgaa agtggaggga atataggcat tggctttgcc cttcctatca
    10201 attctgtgaa gaccattctg gccaaaatat gagcaaaaaa atccccttaa taatgctaat
    10261 attcatagca ggaagtttgc tttgtgcgca aattacacat agctgttatg atattcaata
    10321 taatcctgaa ggaggttcaa attcccccta cctggatcaa gttgtaactg ttcaaggaat
    10381 tgtaagcggg atatcttttt attcaggaac cagcgtaaat agctatggtt tctttattgc
    10441 tgatgcggca ggcggaccct ggagcggttt atatatttac aatcaaacct accatcccaa
    10501 tttgggagat ttagttcaga tcacaggaac agttaatgaa tattatggtt taacagaaat
    10561 ctctcaactt actacttttc aggttttaag ccaggataat ccacttccag aacctgcaga
    10621 aataaatagc ggagccctca ataatttgag cagcggagaa cagtgggaaa gtgttttagt
    10681 gaaagttctt aatgtttccg ttgttaccga accaaataac tatcaggaat tttatgttga
    10741 tgatggcagc ggtgtatgtc agatagatga tcaatttttt ggttctggtc acagctggtc
    10801 taatattaca ttagggcaag tatattctga aattacaggt attgtagatt atgcctataa
    10861 ttactatgct ataaaccccc gctttctttc agaccttgtt ttaagtggga ctacagccac
    10921 attactaatt cctcatcaag cagttagttt acatagctct gttactgttc cagtaaatgc
    10981 tttgggcata gatttagctt ccagctatca atcctattct ttcaacttag ctttcaatcc
    11041 taatattata tcttatcagt ctgttagcac cgagggaaca atttctgccc caggaactat
    11101 taatgcttct tgcacaaacg gtttgctctc cgtttcttat caaagcaatt cagttattag
    11161 cggtcagggt gtacttttaa atctgaactt ttatgcaacc aatacaggaa cgactgtttt
    11221 agccctggaa aatgtgtttt tcggcaacga tagtatccaa agcttaattg atggcagttt
    11281 aaccgttaat agctgttata attcgcctgg tgacacttta acggttatcc agcgtccact
    11341 tcttaatatt ccggaaatcg ttattccggg agaaacatta acgataacct gtttggcacc
    11401 tcaaaataca actaactgga atgcctggct gaaacatagt aataaacgca taaatatgcc
    11461 gatttcaaac agccaatggt taaataatcc taacaggtgg gaacttgtta caactattcc
    11521 atctgttccg atttttgaat tatacgattt ggaggtttct gccagtgggg gaattagcga
    11581 tattacagaa aatgcgattc aggtgattcc tacgcgaaaa aataactatt actttgtgca
    11641 tataacagat gcgcatttac ctaaccgttt atactatccg aatgcaggct tcgaaacgga
    11701 ttctactgca gtagaagatt ttagagcagt gatggaggat atcaatctta ttcatccgga
    11761 atttgtgctt tttacaggtg atttggttaa tgaaggcgag ctggaatctt ttgcgaatca
    11821 atattggtat ggctggacgc aaaaactgct ttccgaattt gatattccag tttatgtaac
    11881 ttctggaaat catgatatcg gcggttggaa tcaaactcca cctccagctg gatctgccag
    11941 aagaaactgg tggaaatatt ttggttggaa ctggttagat agtgaagatc ccaattggct
    12001 atatcatact caggattatt atttcaccta cggaaacaca atttttatcg ggctggaaag
    12061 ctatgataac tatgattatt ggcgtttaaa tatctatggc acaaccagtt ttaccgatca
    12121 acaaattgct tggctaaata gtactctatc tctttttcca aactatacca aggtgctatt
    12181 tcatcactac gattttcagg atgagcttaa tctttctgct ttaggaattg atctggcttt
    12241 gtgggggcat atccattcca ataatggctc aatatctaat ttcccctata atttagccac
    12301 gcgtagtatc tgtgatggaa atcgtgccta tcgaatggtg cgagtgaata ataaccaaat
    12361 tataccctac aatactattt atgctggagc aacaggaaat aacctctcta ttcaatattt
    12421 cccttccaat tatggagtag cggatagcat tagagccact attatcaata atcaacccct
    12481 aacctttgaa aatgcccaaa taaaatttat tatgccttct ggaatgagcg gctatagagt
    12541 tcagggagga attttggaac aggtagatag aagcggggtt aataatgttt gctatgtacg
    12601 cgtttcgctt tctgctaata gcactaccaa tattagtatt gccgatagcg gagtggaaaa
    12661 tatagaccct ctgcaggtgc ctaagccatt acaaataaaa agcatctacc ccaatccttt
    12721 atccctgcaa ggaaatatac aactttatag tgaaaaatcc ttgcctacct taaccttgca
    12781 actatataac ctgaaaggac aactggcata tcaacaaacc ttacaaaaca ttaacaaagg
    12841 ggaaaatacc ctggctttca cattgcccga ttccttagga aacggaatat actttctacg
    12901 cttacctgaa gataaaaaca caacccaaaa aatattaatt cttaaaaagt gagattataa
    12961 tgcctgaaaa aagacctcaa actgctaaac ttctctacat accttggatt ttaaaacatc
    13021 tatcctatta tgtgaaattg aatacccata tccttaaaaa aggggtgcca ctgaaagaaa
    13081 tcctgatctt aaaatttccg ttctggttca gcgatgccaa aattccaccc ttgctttctg
    13141 tggatatcac cgatgcctgc aatttaaaat gtaactattg taataatccc ttcttcccac
    13201 atccccgcac aatgatgagc aaagaagttt ttgaagcatt aattgaacac ctaaaacgcc
    13261 aaaatgttag ccgcatcaga attagcggtg gagaaccaac tctgcatcca caattcgata
    13321 caatgctgaa agaaatgtcc cactactgta aatatctatc aataatcacc aatggtcaat
    13381 ggaaaaataa agaaataaca gaaaaactgc tcgattgtgg acttagcttt atcgaacttt
    13441 ctatggacgc cggaggaaaa gaaatatacg aaaaaaatcg ccccggtgcc gattacgatc
    13501 tcttcttaca aaacctgaaa gacctgtatg aactgcgtaa ccggaaaaat acagacctaa
    13561 taattagaat tcgattaatg ctgcgtcctt ccactctatc cttagaaaaa gaagatagcc
    13621 actattggaa acaattcagc gattgcgttc tgcctcaaca tattttacaa catccggaaa
    13681 caccacccca agaagatgtt tttatgcagg attctatcct ccaaaatcaa cttcctgtct
    13741 gtactgtaac ctatagagat ctgcaaatta gacccgatgg aaaagttcca ctctgccccg
    13801 caaaaggatg tgccataaat ctcgaagacc gtatttttat tggcgatatc caaaaggacg
    13861 atattctgga tctgtggaat gcaccaatgc tgaaacaaat gagaaacgct cacaggaaaa
    13921 gacagggaga aatattaaac ctctgccgta attgccatta tggatgaatg cccactcatc
    13981 ggggaacaaa ataacctcac gcagatcacg cagatttagt tttagaaaca aggattttag
    14041 gattagattt aagaaacgag gatttaagga ttttaggatt aagaggattt tttagaaaca
    14101 aggattctag gattaagccc cttagggcga cacaaagata gcgatggtgg cgtaaataat
    14161 ctgtcggttg tcggctttaa gtcaaaatcg gc
//
LOCUS       DAORHB010000015        14020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3414_length_14020_cov_5.419119, whole genome shotgun sequence.
ACCESSION   DAORHB010000015 DAORHB010000000
VERSION     DAORHB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14020
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3414_length_14020_cov_5.419119"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3850)
                     /locus_tag="PKM80_01000"
     CDS             complement(<1..3850)
                     /locus_tag="PKM80_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNV92515.1"
                     /translation="MSKQTIFSTVHIEGSLLSNEFLQCLINGDQTLEGIKPEDYYLSP
                     TERLNEAATRSYLRLTGVWESFKNHIKDLAEDDIGTTETRERWLYPLFHELGYNALQT
                     QKSIEIEGKFYPISHKSSEPVAIHLISYKWDLDRINPSARGSARRSPHSLMQEFLNRS
                     SEHLWGFLANGYKLRLLRDNVSLTRSSYIEFDLQAMMDGEVYSDFFLLWMLCHQSRLK
                     VDLDDFGKPLPPESCFLEKWYNFSQSEGIRIWDELRDKVKLAIEAFGCGFLSHPSNSI
                     LKENLSSGELTKSDFYHQILHLVYRIIFILVAEERNLLLPPDLPDNLKKIYYQYYSLQ
                     RIKKIAKRKRGTKHSDLYKQMRITFTGLYSGNEILGLPALGSFLFKEESTSALNNCEI
                     ANSDLLTALRFLCYTQKNNVYQEISYRNLGSEELGSIYESFLEMHPDLNIEAGTFVLK
                     VISGSERKTTGSYYTPDSLVNCLLDTALEPVINSALKKAKTEDNSPENQEKALLNLKI
                     CDPACGSGHFLLKAAHRLAKRLASLRAQEEEPSPLVIQNALRDVISHCIYGVDLNPLA
                     VELCKISLWMDALEPGKPLTFLDHHIKCGNSLLGCTPQLLKEGIPDSAFTPLTGDDKS
                     YCAYYKKRNKEERSGVQDIFTPDNKNWSYEQNIAPSQRNLNQMPEENLADLQKKEQAY
                     QELQASESYLNSKFIYDAWCAAFVIKKTEQESYPYLITQGILYKISENPYYIDPNLRK
                     EIIRLADVYQFFHWHLAFPEVFSADIANDDNAEQTNQLPIKKTLGFDCVLGNPPWEKV
                     KLQEKEFFAARDTDIANAPNASKRKELIAALPNTNPDLYQEFRKQLRIAEASSLLIRE
                     GGNFPLCGIGDLNTYTVFAELNRNLLNNIGYCGCIVPSGIATDDTTKMFFSSLIITGS
                     LHSLYDFENRKGIFPGIHRSFKFCLLTIAPLNLEYILNKKNSTEPAIADFVFFATSIA
                     DLKDPERHFKLSQEDIELLNPNTLTCPIFRNKSDAELTKYIYRRVPILINENYGEEGN
                     PWGISFLRMFDMSNDSYLFKTKIELEAEGFELSGNHFTKGEEQYLPLYEAKMIHHFNH
                     RFGDYTDLPEGSVSTKLPRVPLDRLQDPHYEPLPRYWVNIKEILQKLLNPGILFGFRD
                     ITNVTNERTFISSAFPLSAVGNTLPLISSSKYDLLPLLLCNMNSFCCDYLIRQKVGGT
                     HMTYNYVKQFPVLTDNQYPAQLFQFIISRFIELSFTSYGMTNNDVTIYNGPPFIWDEE
                     RRFEIQCELDALYFHLYLGTQ"
     gene            complement(4118..6982)
                     /locus_tag="PKM80_01005"
     CDS             complement(4118..6982)
                     /locus_tag="PKM80_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNV92516.1"
                     /translation="MEQLQPDKSSNDIPEDYAIGSLVSVRGREWVVQPESTSELLIVK
                     PLGGSDDEITGILTKLEEVHPASFSLPNPNELGDYQSCNLLRNALRLGFRNSAGPFRS
                     FGRLAVNPRPYQLLPLLMALKLDPVRILIADDVGVGKTIEACLIARELLDRGEISRIC
                     VLTPPHIAEQWQSELLDKFHIDAELVLSSTVRQLEKNCAQNESVFEVYPFTVVSIDYI
                     KSGRHKDEFIRTAPEMIIIDEAHTASFDSVSTSSRHQRYELVNALSKDPLRHLILVTA
                     TPHSGNVNAFRSLLSLLNSEFTAYPDDLTGTENENYRKTIAKHFIQRRRADIEHYLED
                     TSFPEAIIEELSYKLSEPYRQLFSKALNYAREIVQDKEGGDLHRRIRWWSALSLLRAL
                     ASSPAAALKTMNNRNATQETDSPFEADELGRRQVYDTYDRDATESSDIVPGSISSEEE
                     NSYSNKKLRALAQEAESLFGELDYKLLNIINPLKTYLKEGFKPIIFCRFIQTAEYLTD
                     QLKQRLPQDVEISAVTGVLPPEEREKRIIELSKHKKRVLVCTDCLSEGINLQNYFDAV
                     IHYDLSWNPTKHEQREGRVDRFGQTSKKVKLLTYYGTDNQIDGIILDVLLKKHHQIKS
                     TLGVSVPVPYDSEQVLEAIFEGLLLREQESSSEQLLLPFLDDFLAQDKQKLHLAWDKA
                     KDREKLSRSIFSQQALSKDLKEITTKELDKINESIGSRLDIRNFVKHSLIRFNASIIE
                     HEDTLQIDASAVPTFVKESCYFPSDKLTLKFELPVSENEIYLPRTHPIVEGLADIAIN
                     TALEGNSAIAKRCGVIRTSSVERKTTLLLMRYRFHLIEAFHHKSYPILVEDSQILAFQ
                     GKPEEPEWLENKEISKLLESIPAVNVSPEFAKEQLENVILNYETLLPHLNTFAEKRAR
                     ELLSAHTAVRDLSLIRKSVKPYVKALLPADLVGVYIYLPV"
     gene            complement(7060..12186)
                     /locus_tag="PKM80_01010"
     CDS             complement(7060..12186)
                     /locus_tag="PKM80_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV92517.1"
                     /translation="MNIFELRDKIIEEYSSFVNGFINIKDSKASNYVLDKINKGLLWP
                     DPLVQINPFFTLGESVKELVSQGFLHPDCEKIFCLRNEKEIGSKPLQLYKHQSDAIKK
                     AVANESYILTTGTGSGKSMAYIIPIVNHILQTGSGKGIKAIIIYPMNALANSQKQELE
                     KFINPHHKSSANNISFRCYTGQENDAERQEILANPPDIILTNYVMLELILTRPDETPL
                     VSASKNLKFLVLDELHTYRGRQGADVAMLIRRVKEATGTKDVIFVGTSATLSSGDIET
                     QKQEIAKVSSLLFGIDFNQGNVIGESLERITAEYDFTNRDELELLKKEISEVINVPKL
                     EFEQIKHSHLASWIESNFGIRTDPKTGILVRSEPISITGPKGGARLLHNLTDKENELC
                     VRAIQKMLMLGFQTLNPINSFPVFAFRLHQFISRGQTVYASLEDNDSRYFTMQKQLYV
                     PDTNKLKVLLPLTFCRFCGQDFYTVHRTLNKDNETLYTPRELYDIYLYEEDAGFLYLP
                     DNPDCDLTAMIPPEWKDINGNIIAARKYDMPEKVSVRPDGIENPAGRIMYFFKTPFRF
                     CPSCGVSFDATQRSDFPKLTALGTEGRASATTILSLSVIRNIKQMDMEDKAKKLLSFT
                     DNRQDASLQAGHFNDFIQIGLLRGALYQALVNAGSDGIRHSELTQKVFDCLNLNYEEY
                     ASNPLADRDSARIYTERALCDVLGYRLYYDLRRGWRITAPNLEQTGLLKIDYLALEEL
                     AYSEEEWQNCHPALSSCDHQARYNILKTLLELMRRSLTIKVNYLDPRFQDIMLQNSHQ
                     RLIPPWGFDESTQSRNLTYASILYLDPNSNARNRGSNLYVTPRSSYGQYLRRATTFKD
                     YKTTIDLQQTKTIIENLIDRLTRYGIIEKVDDKNNGYQLVADSIIWKQGDGKTGYYDP
                     LHQPTESLEGQPVNEFFKEYYSTIALTTHKIYAREHTAQVSYEERKKRETAFREGTLP
                     ILYCSPTMELGIDISWLNVVNMRNIPPTPANYAQRSGRAGRSGQPALILSYCSTGSPH
                     DQYFYKHPEKMVSGVVSTPQIDIANENLLHSHINAIWLSEAKLSLGRALNNIIDISTE
                     KLELLPSVKSTLNNSELQNRAFERAESVIATVQNYLQSTIWFNNDWLTDVIKQIPNEF
                     EQACERWRDLYRAALNQQQLQNSIILDVSRSAADRKQAIQLRKEAETQLNLLTSIEGA
                     FQSDFYSYRYFASEGFLPGYNFPRLPLSAYIPARGTVKGEDEYLNRPRFLAITEFGPR
                     SLIYHEGSKYIISQVIMPPTRDNNNLPSLASMKLCAECGYLNGYENDICENCKAQLDS
                     PLTNLFRMQNVVAKRREQISCDEEERLRMGYEMRTTIRFAKKGGKLSCFQTELYSENE
                     LLAKLTYGDSADIWRINMGFKKNHRGRQLGFWLDSERGFWERNPDTDNEDDSPLGNRR
                     ILVCPYVSDTKNCLLFEPQFNAEPAVMASLQSALKNAIQQVFDIEDNELAAEPLPNRN
                     VRHSILFYESAEGGAGVLKRLMDEQNFRKTIRKALEICHYDPDSFTDLEMLKNASERC
                     EAACYDCLLSYKNQLDHLLLDRKSIIIHLKEMLNADFKRSSSEVTRLELYHQLRNLCG
                     SELESKWLDFIYNNNYILPLHAQQLIEKAQTRPDFTYPDIYVAIYIDGQVHDFSNNIE
                     KDREVDARLDDLGWKVIRFLYNQDWQTIIDQHPEIFGTRK"
     gene            complement(12431..13771)
                     /locus_tag="PKM80_01015"
     CDS             complement(12431..13771)
                     /locus_tag="PKM80_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV92518.1"
                     /translation="MKYINLLLAFLVAGILFAQPNFMPVTTIAEDFGSLSCPNCLVAW
                     QGLQAVHSETHNGEFITARLFTESGSLSNAEIEERFSYYGVIGLPNVIFNGLISIEGA
                     GEGTADGTNYLNALSQTRFSASPVKIDITDFSLGRGAVSANVTMLSPTAVISNAKLVY
                     YLLEDNVGSETNVLRGVLYDDIASLSGAGTTLNFNRTFDLNPDWSVSNLWGLIFIQLS
                     DKTIVQCVSSLPLPEYNFRVAMDWKTFVTVEPNTNYLSSPIWLFNLGAADNYTMSIQV
                     DNAPADWYFNYCDEEGNCYPGSIDRPFTLAAGEATSIHLNLMIGSSGTADFRIAVNSP
                     HIGSYNVPFHITASTPIIDDTNTLSPYIIKGFYPNPVKSSAVFHLSNPKNVGSATIEI
                     YNVKGQKVQTLKETNLHSGENEISFVPDTNLPNGIYYYRLQNSNSKAGRFIIMK"
BASE COUNT         4296 a         2607 c         2686 g         4431 t
ORIGIN      
        1 gctgcgttcc caaatagaga tgaaaataaa gcgcatccaa ttcacactga atttcaaaac
       61 gacgctcctc atcccaaata aaggggggtc cattatatat ggtaacatca ttattggtca
      121 taccatacga agtaaaggaa agttctataa atcttgaaat gataaactgg aagagttggg
      181 caggatactg attatccgtt aatactggaa attgcttaac ataattatag gtcatatgtg
      241 tcccacctac tttttgccta attagataat cacaacaaaa actattcata ttgcacaaaa
      301 gtaatggcaa tagatcatat tttgagctac taataagtgg aagagtattt ccaacagcac
      361 ttaaagggaa agcagatgat ataaaagtcc tttcgtttgt tacatttgtg atatctctaa
      421 agccaaataa aatccctgga ttaagtagct tttgcagaat ttctttaata tttacccaat
      481 agcggggtaa gggttcgtaa tgtgggtctt gtaatctatc taatgggact ctgggtaatt
      541 tagtggaaac cgaaccttca ggtaaatcag tataatcccc aaaacgatga ttgaaatgat
      601 gaatcatttt tgcttcatat aggggtaaat attgttcttc tcctttggta aagtgattac
      661 cggaaagttc aaagccctct gcttccaatt ctatttttgt tttaaagaga taagaatcgt
      721 ttgacatatc gaacattctt aaaaatgata ttccccaagg gtttccttct tctccataat
      781 tttcattaat aaggatagga acccggcggt agatatattt agtaagttcg gcatcgcttt
      841 tatttctgaa aatagggcag gtaagggtat tgggattaag caattcaata tcttcctggc
      901 tcagcttaaa atggcgttcc gggtctttta aatcggctat ggaagtagca aagaagacaa
      961 aatcggcaat tgcaggttct gttgaattct tcttatttaa gatatattca aggttaagag
     1021 gagcgatagt taaaaggcag aatttaaagc tgcgatgaat tcctggaaat atgcctttcc
     1081 tgttttcaaa atcatagagg gaatgcagag agccagttat aattaaagaa ctaaagaaca
     1141 ttttagttgt atcatcagta gcaataccac tgggaacaat acaaccacaa taacctatgt
     1201 tgtttaaaag gttacgattt aattctgcaa agacagtata ggtattcaga tcacctatac
     1261 cgcaaagagg aaagtttccc ccttctctaa ttagcagaga agaagcttcg gcaatacgaa
     1321 gttgttttct aaattcctga taaaggtcag gattagtatt aggtaaagca gcaattaact
     1381 ctttgcgttt tgaggcattg ggggcattgg caatatcagt atcccgagct gcgaaaaatt
     1441 ctttttcttg cagtttaact ttttcccaag gtggatttcc gaggacacaa tcaaagccga
     1501 gtgttttttt tataggcagt tgattagttt gttcggcatt atcatcgtta gctatatctg
     1561 cagaaaagac ctctgggaag gcaagatgcc agtggaagaa ttgataaaca tcagccaggc
     1621 ggataatttc ctttcgcagg ttaggatcta tgtaataagg attttcgctt attttataca
     1681 aaatgccttg ggtgattaga tagggatagc tttcctgttc ggttttttta ataacgaaag
     1741 cagcacacca ggcatcgtaa ataaatttac tgtttagata ggattcggag gcctgaagtt
     1801 cctgatatgc ctgttctttc ttttgcaggt ctgccaggtt ttcttccggc atttggttta
     1861 gatttctttg ggaaggtgca atattttgtt cataactcca atttttatta tcgggggtaa
     1921 aaatatcctg aactccgctg cgttcttctt tatttctttt tttgtaatag gcgcaatatg
     1981 atttatcgtc tccggttaga ggtgtaaaag cggaatccgg aattccttct ttcagcaatt
     2041 ggggagtgca gcccaaaaga gaatttccgc atttgatgtg atggtcaaga aaagtgagag
     2101 gttttccggg ttccaaggca tccatccaaa gagatatttt acaaagttca actgccaaag
     2161 gattgagatc gacaccataa atgcaatgac tgataacatc ccttagggca ttttgaataa
     2221 ctaaggggga tggttcttct tcttgagcac gaagggaagc cagtcgtttg gctaaacgat
     2281 gagcagcttt caataaaaaa tgtccgctgc cgcaggcagg atcacaaatt ttaagattca
     2341 gcaatgcttt ttcttggttt tcgggagaat tatcttctgt ttttgccttt ttcagagcag
     2401 aattgataac tggttctaat gctgtatcca gtaaacaatt aactaaggaa tcgggagtat
     2461 aataacttcc ggttgtcttt ctttctgaac cggagataac tttcaagaca aaggttccag
     2521 cttcaatatt aaggtcagga tgcatttcca gaaaggattc atagatactg ccaagttctt
     2581 cggaacccag attgcgataa ctgatttcct gataaacatt gttcttttga gtataacaaa
     2641 gaaaacgcag tgcggttaat aagtcgctat tggctatttc acaattgttt aaagcggaag
     2701 tagattcttc tttaaataga aatgaaccca aagctggtaa gcccaaaatt tcgtttccac
     2761 tataaagacc tgtaaaagta atccgcattt gtttataaag gtcactatgc ttggttcccc
     2821 tttttctttt ggcgattttt tttattcttt gcagagagta atactgataa tatatctttt
     2881 taaggttatc gggtaaatct ggagggagca gaagatttct ttcttctgca accaaaataa
     2941 aaattatgcg atagaccagg tggaggattt ggtgataaaa atcggatttg gtaagctctc
     3001 cagagcttaa attttctttt aatatagaat tggaaggatg agaaaggaat ccgcaaccaa
     3061 atgcctcaat tgccagctta actttatccc gcaattcatc ccatattcta ataccttcag
     3121 attgggaaaa attataccat ttttccagaa aacaactttc aggaggcaag ggttttccaa
     3181 aatcatcaag atcaactttt aaccgcgatt gatgacacag catccaaagc aggaaaaaat
     3241 cgctatagac ctccccatcc atcattgcct gcagatcaaa ttctatataa gaactgcggg
     3301 ttaaagaaac attatctctt aagaggcgta atttataacc attggcaaga aacccccaaa
     3361 gatgttcaga actcctattc aaaaattcct gcatcaaaga atggggcgac cttctggctg
     3421 atcctctggc agaaggatta atgcggtcta aatcccattt gtaactaatt agatgaatag
     3481 caacgggctc tgaacttttg tgactgatag gatagaactt tccttcaatc tcaatggatt
     3541 tttgagtttg taacgcatta tatcccaatt cgtgaaaaag aggatataac cagcgttctc
     3601 ttgtttcagt agtcccaata tcatcctctg caaggtcttt aatatgattt ttaaaacttt
     3661 cccacacacc ggttaatctt aggtaggaac gagttgctgc ttcattcaaa cgctccgtag
     3721 ggcttaaata atagtcctct ggttttattc cttccagggt ttggtcaccg tttattaagc
     3781 attgtaaaaa ctcattagat aaaagagagc cttctatatg cacagtggaa aagattgttt
     3841 gtttagacat tagcatccta ccttataatt taaactcaca tagctaagag agtttggcgt
     3901 attaggggtt agaacccaga taaaaattag ctttgcagct tctattaagc tataagctac
     3961 agagtgaaga acaaaaacaa caagattaca tatagtacta agaggaaaga tagtttcttc
     4021 cctttccaga aggttttctg cattttggct tagataatta ggcaattcca ttctttacta
     4081 ctctctatta taataatctg aattaacttt agaggtttta aactggtaga tagatatata
     4141 cacctaccag gtctgctgga agtaaggctt ttacataggg tttaacagat tttcttatta
     4201 aagataaatc ccgcactgca gtatgagccg ataaaagttc ccgggctctt ttttcagcaa
     4261 aagtattcaa atgaggcaga agtgtttcgt aatttagaat aacattttcc agctgttctt
     4321 tggcaaattc aggagagaca ttaacagccg ggatggattc aagtagtttg cttatttctt
     4381 tgttttccag ccattcaggt tcttcaggtt taccttgaaa agcaagaatc tgagagtcct
     4441 ctactaagat aggataggat ttgtgatgaa atgcctcaat taaatggaaa cgataacgca
     4501 ttaacagaag cgtggttttt cgctcaaccg aagaggttct aattactcca catctttttg
     4561 caattgcgct gttgccttcc aaggcagtat ttatagctat atctgccaat ccttctacta
     4621 taggatgagt acggggaaga tatatttcat tttcactaac gggaagctcg aattttaggg
     4681 ttagtttatc ggaaggaaaa taacagcttt ctttaacaaa ggtcggtaca gcggaagcat
     4741 caatctgtaa tgtatcttcg tgttctatta tagaagcatt aaagcggatt aagctatgtt
     4801 ttacaaaatt tctgatatcc aacctggaac cgattgattc gttaatttta tccagctctt
     4861 tggttgttat ttcttttaag tctttggata atgcctgctg agaaaatata gaccgactaa
     4921 gtttttcccg gtctttggct ttatcccaag ccagatgtaa tttctgttta tcttgggcta
     4981 ggaaatcgtc aagaaaggga agcaaaagct gttctgaact gctttcctgt tcccttaaaa
     5041 gcagaccttc aaaaattgct tccagaacct gttcggaatc gtaaggaacg ggaactgaaa
     5101 cacccaaggt agattttatt tgatgatgtt tttttagcag aacatccaga ataatgccat
     5161 ctatttgatt atccgttcca taataagtta gcagcttcac ttttttgctt gtttgtccga
     5221 aacggtctac ccttccttct ctttgttcat gtttggtagg attccagctg agatcatagt
     5281 gaattacagc atcaaaataa ttctgcaggt taattccttc agaaagacaa tcggtacaaa
     5341 ccaaaacccg ttttttatgt ttggaaagtt caattattct tttttctctc tcttctggag
     5401 gtaaaacacc cgtaacagca gaaatttcta catcttgggg taaacgctgt ttgagctggt
     5461 cggtaagata ttcagcagtt tgaataaagc ggcaaaagat gataggtttg aagccttctt
     5521 ttagataggt ctttagtgga ttaattatat ttagcagttt atagtccagc tctccaaaaa
     5581 gggattctgc ttcttgggca agagctctta gttttttgtt actgtaagaa ttttcttctt
     5641 cggaggaaat actaccggga acaatatcac tgctttctgt ggcatcgcga tcataagtat
     5701 catacacttg gcgacgaccc aattcatcgg cttcgaaggg actatcagtt tcctgagtag
     5761 cattgcggtt attcattgtt tttaaagcag cagcaggact ggaagctaaa gctcgtaata
     5821 aagaaagtgc agaccaccat ctaatccttc tgtgaagatc acccccttcc ttatcctgga
     5881 caatttctct ggcataattc agtgctttgc taaaaagctg acgatagggt tcggagagtt
     5941 tataactaag ttcttcaata atagcttcag gaaaagaagt atcttccaga taatgttcaa
     6001 tatcagctct gcgacgctgg ataaaatgtt tggcaattgt cttacgataa ttttcgtttt
     6061 cagttcctgt tagatcatca ggataagcgg taaattcact gttcaacaaa cttaataggg
     6121 aacgaaaggc attcacattt cctgaatggg gagttgccgt taccaaaata agatgacgca
     6181 aggggtcttt acttaatgcg ttaaccagtt catagcgttg atgtctgcta cttgtgctaa
     6241 cactatcaaa actggcagta tgagcttcgt ctataataat catttctgga gctgtgcgga
     6301 taaattcatc tttatgacga ccgcttttaa tataatcgat ggaaactacc gtaaagggat
     6361 agacctcaaa gacggattca ttttgggcac aattcttttc caattgacgc actgtgcttg
     6421 ataatactaa ttctgcatca atatgaaatt tatccaataa ttcgctttgc cattgttcag
     6481 ctatatgagg gggagttaaa acacaaattc ttgatatttc acccctatcc aaaagttctc
     6541 gggctattaa acaggcttca atggttttac caacacctac atcatcggca atcaaaattc
     6601 taacgggatc aagctttaaa gccatcagta agggtaataa ttgataagga cgggggttaa
     6661 cagccaatct gccaaaactt ctaaatggac ctgcgctgtt tctaaaccct aatctgagag
     6721 catttcgcaa gagattgcag gattgataat cgcccaattc attaggattg ggcaaactga
     6781 aagaagcggg atgaacttct tctaatttgg ttagaatacc ggtaatttcg tcatcactgc
     6841 ctcctaaggg tttaacaatt agaagttcag aggttgattc tggttgaaca acccattccc
     6901 tacctctaac agaaacaagt gaaccgatag cataatcttc aggaatatca tttgaagatt
     6961 tgtctggttg aagttgttcc actttttctc cttttcaaga tgatataggt aatttatcta
     7021 taaccggttt tatggtgtta gttataaacc ggaacatatt cattttctcg ttccgaaaat
     7081 ctccggatgc tgatcaatta tagtttgcca gtcttgatta taaaggaatc ggattacttt
     7141 ccagccaaga tcatcaagac gagcatcaac ttctctgtct ttttctatat tattggaaaa
     7201 atcgtgaacc tggccatcaa tgtaaatagc aacatatata tcagggtaag taaaatcggg
     7261 gcgtgtttgt gctttttcaa ttaactgctg ggcgtgaagg ggcaggatat agttattatt
     7321 atagatgaaa tccagccact tactttccaa ctcggaacca cacaaatttc gcaattgatg
     7381 gtaaagctca aggcgagtaa cttcactgga ggatctctta aaatcagcat taagcatctc
     7441 cttgagatgg attataatag attttcggtc taaaaggaga tgatcgagct gattcttata
     7501 gcttaaaaga caatcataac aggctgcttc acatctttcg ctggcatttt tcagcatttc
     7561 caaatcggtg aaactatccg gatcataatg acatatttca agcgctttcc tgatagtttt
     7621 acgaaagttt tgttcatcca tcaatcgttt tagaactcct gcaccacctt ctgcagattc
     7681 ataaaataaa atcgaatgtc tcacattcct gtttggtaaa ggttctgctg ccagttcgtt
     7741 atcctcaata tcaaaaacct gttgaatagc attctttaaa gcagattgta aagaagccat
     7801 aacagcaggc tctgcgttaa actggggttc aaaaagaaga cagtttttag tgtcgctaac
     7861 ataagggcag accaatattc ttctgttgcc taaaggcgaa tcgtcctcat tatcagtatc
     7921 aggatttctt tcccaaaaac cgcgttcact atccagccag aaacctaatt gcctacctct
     7981 atggttcttt ttaaaaccca tattgatacg ccagatatca gctgaatctc cataagttag
     8041 ttttgctaaa agctcatttt cagaatataa ttccgtttgg aaacaggata gtttacctcc
     8101 ctttttggca aagcggatag tagtcctcat ttcatatccc attcgtaacc tttcttcttc
     8161 gtcacagcta atttgttctc ttcttttagc aacaacattc tgcattcgga aaagattagt
     8221 taaaggcgaa tcaagttgag ctttacaatt ttcacaaata tcattttcat atccatttag
     8281 atatccgcat tcggcgcata acttcataga agctaaagag ggtagattat tattatctct
     8341 ggtaggaggc ataataactt gagatattat gtatttagaa ccttcatggt agataagaga
     8401 gcggggtcca aattccgtga tagctaaaaa tctgggtcta ttaagatatt cgtcttcgcc
     8461 tttaactgta ccacgagcag gtatataagc agataaaggc aatctgggaa agttatatcc
     8521 aggtaaaaaa ccttctgaag caaaatagcg ataactataa aaatcagatt gaaaagcacc
     8581 ctctattgaa gttaacaaat ttagctgagt ttctgcttct tttctaagct gtatagcttg
     8641 ttttctatca gcagctgatc tgctaacatc taaaataata ctattttgta attgctgctg
     8701 atttaaagca gcccgataga ggtctctcca acgttcacaa gcctgttcaa actcgttggg
     8761 aatttgctta attacatctg taagccagtc gttattgaac caaatggtac tttgtagata
     8821 attttggact gtagcaatca cactttctgc tcgttcaaaa gccctgtttt gcaactcaga
     8881 gttattcaag gtagatttta cagaaggtaa aagctctaat ttttccgtac taatatcaat
     8941 aatattgttt aaagctctac ccaaacttag ttttgcttca gacaaccaaa tggcattgat
     9001 atgagaatgt aacaggtttt cattagcaat atctatctga ggtgtggaaa caactccgga
     9061 aaccattttt tcgggatgct tatagaaata ttggtcgtga ggacttcctg tgctacagta
     9121 acttaaaatt aaggcaggtt gtccactgcg tcctgctcta ccactacgct gagcataatt
     9181 tgccggtgtg ggaggaatat tacgcatatt aacaacattt agccaggaaa tatcaattcc
     9241 taattccatt gtgggagaac agtataaaat tggtaaagtc ccttctcgga aagcagtttc
     9301 tctttttttg cgctcttcat aactaacctg agcagtatgt tcgcgagcgt atattttatg
     9361 ggtagttagg gcaatagtag aataatattc tttgaaaaat tcatttaccg gctgaccttc
     9421 caaagattca gtaggttgat gaagagggtc gtaataacca gttttcccgt caccttgttt
     9481 ccagataata gaatcggcaa ccaactgata tccgttattt ttgtcatcaa ccttttctat
     9541 tattccatag cgcgttaacc tatctatcag gttttcgatg atagttttgg tttgttgtaa
     9601 atctatcgta gtcttataat ccttaaaggt ggtggctctg cgtaaatatt ggccataaga
     9661 actacgaggg gtaacatata aattacttcc ccgatttcgc gcattgctat taggatcgag
     9721 ataaagtata gaagcatagg ttaaatttcg ggattgtgtg ctttcatcaa aaccccaagg
     9781 aggaataaga cgttgatggc tgttttgtaa cataatatcc tgaaaacggg gatcaagata
     9841 atttactttt atcgttaaag atctgcgcat tagttccaac agagttttca ggatattata
     9901 tcttgcttgg tgatcacagg atgacaaagc aggatggcaa ttttgccatt cttcttctga
     9961 atacgctaat tcttccagag ccaagtaatc aattttcagc aggcctgttt gttctaaatt
    10021 gggtgctgta attctccatc ctcttcgcag atcataatac agacgataac ctaaaacatc
    10081 acagagagcg cgttcagtat aaatacgggc agaatccctg tccgcaagag ggtttgaggc
    10141 atattcttca taatttagat taagacaatc aaatactttt tgggttaatt cgctgtgacg
    10201 aattccatcg ctacctgcat taactaaagc ttgatacaaa gctccacgca ataaaccaat
    10261 ttgaataaaa tcattaaaat gaccagcttg aagagaggca tcctgacggt tatcggtaaa
    10321 gcttaataat tttttggctt tatcttccat atccatttgt tttatattgc ggataacgga
    10381 aagggaaaga atcgtggttg ctgaagctct tccttccgtt cccaaagcgg ttaacttggg
    10441 aaaatcactg cgttgggtag catcaaaaga cacaccacag gaagggcaaa atctaaaagg
    10501 agttttaaaa aagtacatta tcctgccagc ggggttttca atgccatcgg gtctaacaga
    10561 tactttttcg ggcatatcat atttacgcgc agcaatgata tttccattga tgtctttcca
    10621 ttcaggtgga atcatagcag ttaaatcaca atcaggatta tccggaagat aaagaaaacc
    10681 agcatcctct tcatatagat agatatcgta caattcacgg ggagtgtata gagtttcgtt
    10741 atccttattt agcgttctat gcactgtata aaaatcctga ccgcaaaaac ggcagaaggt
    10801 aagtggtaat agaactttta atttgtttgt atcgggaaca tataattgct tttgcattgt
    10861 gaaatagcga gaatcattat cttccagaga agcatataca gtttgacctc tgctgataaa
    10921 ctgatgaagt cgaaaagcga agacaggaaa gctattaata ggattaagtg tctgaaagcc
    10981 taacatcaac attttttgta tggcacgaac acaaagttca ttttctttat cagttaagtt
    11041 atgcaataac cgagctccgc ctttgggtcc ggttatgctg attggctcag aacgcactaa
    11101 aattcctgtt ttaggatcgg ttctaattcc aaaattgctt tctatccagg aagctaaatg
    11161 gctgtgtttg atttgttcaa attccagctt aggaacattt ataacctccg agatttcttt
    11221 ttttaacagc tcgagttcat ctcgattagt aaaatcgtat tcagctgtaa tcctttccaa
    11281 agattcacca ataacatttc cttgattgaa atcaatccca aaaagaagag aagaaacctt
    11341 ggcaatttcc tgcttttgag tttctatatc gccacttgat aaagtagcag aagtaccaac
    11401 aaaaataaca tcctttgttc ccgtagcttc ttttactctt ctaattaaca ttgcaacatc
    11461 cgcaccttgc ctgccacgat atgtatgaag ctcatccaaa actaaaaact tcagattttt
    11521 agaagcactc accaaaggag tttcatctgg ccgagttaaa ataagttcca acattacata
    11581 gttagtaaga ataatatcag gtggattggc cagaatttct tgtctttctg cgtcattttc
    11641 ctggcctgta taacatctga aacttatatt gtttgctgaa gatttatgat ggggattgat
    11701 gaatttttcc agttcttgtt tctgactatt ggctaaggca ttcatcggat agatgataat
    11761 tgctttgatg ccctttccag aaccagtttg aagtatatga tttactattg gaattatgta
    11821 tgccatactt ttgcctgaac cagtacctgt agttaagata taactttcat tggcgacagc
    11881 ttttttgatt gcgtcggatt ggtgtttata caattgtaag ggcttactac caatttcctt
    11941 ttcatttctc aggcaaaaga tcttttcaca atcaggatgc aagaaacctt gggaaaccag
    12001 ttcttttaca gattctccta aggtaaaaaa aggatttatc tgcacaaggg gatcaggcca
    12061 caataaaccc ttattaattt tgtctaagac ataattgctg gctttactat cctttatatt
    12121 gatgaaacca ttaacaaatg aactatactc ttcaattatc ttgtctcgga gttcaaagat
    12181 attcattgct atttctccta ttgcttaaag cttatttggt aaaaaaaaca tattaacttg
    12241 tcaagggaaa tctctcaaca taaaaaaagc agacacatat ttcaaattac gaactgactt
    12301 ttacaaatag cttgggtgtt cagaatgcta agttacgggc agagatgatg agtcctttgt
    12361 gcctgtagaa ttcaccaaaa aggtaaaata atcgaggcac aaagggcttc tcacttgtta
    12421 agcagggggc ttatttcata ataataaagc gtccggcttt gctgttacta ttttgcaagc
    12481 ggtaataata gatcccgttt ggcaaatttg tatctggtac aaagctgatt tcattctctc
    12541 cgctatgcag gttagtttct ttcaatgttt gcactttctg gcctttcaca ttgtatattt
    12601 caatggtggc agatcctaca tttttagggt tagagaggtg aaacacagca gaacttttaa
    12661 ccgggttagg gtaaaaccct ttaataatat agggggatag agtattagta tcatcaataa
    12721 taggggtgct tgcagttata tgaaagggaa cattgtaact gcctatatgg ggactattta
    12781 cagcaattcg gaaatcagcc gttccggaag agccaatcat caggtttaga tgaatggagg
    12841 tagcttctcc ggcagccaga gtaaaaggac gatcaattga accaggatag caattccctt
    12901 cttcgtcaca atagttaaaa taccaatctg caggggcatt atctacttga atggacattg
    12961 tgtaattgtc tgctgctccc aaattgaata accatatagg ggaagaaaga taattggtgt
    13021 tcggttcaac agttacaaaa gttttccaat ccatagctac tctgaaatta tattcaggca
    13081 aaggaagaga agatacgcat tggactatgg ttttatcgga aagctgtata aagattaatc
    13141 cccagagatt actgaccgac caatcagggt tcaaatcgaa ggttcgattg aaattaaggg
    13201 tggttccagc gccgcttaag gatgcaatat catcgtaaag tacacctcta aggacattag
    13261 tttcgctacc aacattatct tccagcagat agtataccag cttggcatta gaaattacag
    13321 cagtaggaga aagcattgta acattggcac ttacagctcc tctccctaaa ctgaaatccg
    13381 taatatcaat ttttacaggc gaagcagaaa agcgagtttg agatagggca ttaagataat
    13441 tcgtgccatc tgcagttcct tctcctgctc cctctataga tattaaacca ttaaagatta
    13501 cattgggtaa accgatcact ccataataac taaatctctc ttctatttct gcattgctca
    13561 aagacccgct ttcagtgaat aaacgggctg tgataaattc gccattatgg gtttcggaat
    13621 gcactgcctg taaaccctgc caagcaacaa gacaatttgg acaagacaga gaaccaaaat
    13681 cttcggcaat agtggtaaca ggcataaagt tgggttgggc aaataaaatg cccgcaacca
    13741 ggaaagccag tagtaaatta atatatttca ttttatgctc cttgtgagtt atataataac
    13801 cgattatggg aagttgacaa ccaaaaatat gggaattaat atcacgcgtt ctttctatca
    13861 aacaggtctt agggatgtat ggaagctata tcggcagtga tgattaggaa cagatgcaat
    13921 tacctgatag ttcgataacc ctgtgttcat cccaaaccta ttctatctgc gtgaaatatc
    13981 ctatttaaaa gcgggtgtta agctctatcc gtaaaccttc
//
LOCUS       DAORHB010000016        13305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3715_length_13305_cov_3.980981, whole genome shotgun sequence.
ACCESSION   DAORHB010000016 DAORHB010000000
VERSION     DAORHB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3715_length_13305_cov_3.980981"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1137)
                     /locus_tag="PKM80_01020"
     CDS             complement(25..1137)
                     /locus_tag="PKM80_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HNV92519.1"
                     /translation="MINKIKGQEDALKLLRKAIENERISQAYLFYGSDGVGKFTTALY
                     FGMAVNCMATNELKPCGHCDSCHKFLQLEHPDFVYIFPTPNLNLSPEGEIKNSDALKM
                     FQKYIENKVNTPWADFFFKDTIEIRKESITMLSKHFELSIHEARYRIVIVEDADMMTI
                     ATANAFLKTLEEPPERTIIILITERIQKLLPTILSRTQPIYFRPLTRSVIEEILTTEF
                     EISAPIARTASRISAGNLKTAIRVASDSVSLSRNWAFEIFALAATKDDLGYLGLAKAY
                     KDQQKKDQVIDLLKYIRIIASDLGALNANFEAEITNIDKIDDLKAMVENKSGIDDRLY
                     DFLLFLEDLNRKIEGNVNLNLVMTNLYLNCKHLLNS"
     gene            complement(1138..1743)
                     /locus_tag="PKM80_01025"
     CDS             complement(1138..1743)
                     /locus_tag="PKM80_01025"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNV92520.1"
                     /translation="MKYFRKLVGEKCYLSPVSMDDVEKYTEWLNDMEIGQFVTLSHQV
                     IDIEKESELLRELMKNNCIFAIVEKDTNKVIGNCGIHQVSDVHRNASLGIFIGEKTFW
                     NQGIGSEATNLLLDFAFNMLNMHNVYLSVLAYNKRAIRCYEKIGFKKVGVQREFMFVS
                     GQYHDVIIYDMLASEFTSPYIKKVYTYSVSDEAGRSKISIV"
     gene            2717..2802
                     /locus_tag="PKM80_01030"
     tRNA            2717..2802
                     /locus_tag="PKM80_01030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2751..2753,aa:Leu,seq:tag)
     gene            2834..4126
                     /locus_tag="PKM80_01035"
     CDS             2834..4126
                     /locus_tag="PKM80_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNV92521.1"
                     /translation="MQVEFNQINVVIKEINITIPSDEVTKAYEKYLVKIAKEIEVPGF
                     RKGKAPLYMVERVNTEKIKENFCYDYVIDVFDKVAEENHIEYLLYPDIKDLQWEKGND
                     MTVKIQIEHEPQLELKQIEGLTVPYKPAILEESVEKFIADLIAQNTIIKDVEIAEEND
                     EVEVELIVQNNSDTYTVSAIFYACSEDNDYSIPALIGHKIGDTLSLILSGKKIQTNAL
                     NADFNLVDDTEYSCELCINRISRKEIPALDDEFAKDMEYDSLEEMRAKIAEEMKLRIE
                     HINIDIENNSIINKLFTDNPFPLPMKTVEYLAFQQISDVSDDNLRKLLLNRYYYMIMN
                     QMVQLYIGNALMKEKPIELTDEMVEEYINHQAIIQDVTVEAYKEHSKIDFSSQDTQNS
                     IKEYFILRQIAKTCEFVIEKEPPETENTESGTEKIEEE"
     gene            4126..4710
                     /gene="clpP"
                     /locus_tag="PKM80_01040"
     CDS             4126..4710
                     /gene="clpP"
                     /locus_tag="PKM80_01040"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNV92522.1"
                     /translation="MSFVPIVIEQVGRSERAYDIYSRLLKDRIVFVGGVIEDNLANTV
                     VAQLLHLEGEDPERDIYMYINSPGGVISAGLAIYDTMQYIKPRVSTICIGQAASMAAV
                     LLAAGAPGKRTALPNSRIMIHQPMGGAQGQATDIEIQAKEILYLRGRMNEILHKHSGQ
                     TIDKIREDTERNYFMSAEEAQEYGIIDEVISMRK"
     gene            4800..6047
                     /gene="clpX"
                     /locus_tag="PKM80_01045"
     CDS             4800..6047
                     /gene="clpX"
                     /locus_tag="PKM80_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNV92523.1"
                     /translation="MEKYRTLTCSFCGRAEHEVKNLIRGIAGNICDECIVMCHTIIDS
                     SKEEISQEEFDAPVPSRIKAYLDQYVIGQDNAKEIISVAVYNHYKRIFKRKYKDDIDL
                     EKSNILMIGPTGSGKTLIAQTLARFLQVPFAISDATTLTEAGYVGEDVENILVRLLQN
                     ANYDIAKAERGIVYIDEIDKISRKSETPSITRDVSGEGVQQALLKILEGTKVNVPPKG
                     GRKHPQQDFIEMDTKNILFIAGGAFFGLEKIIKERMDKKSVGFDVELGKRQDEATIFD
                     YTTPQDLLKYGLIPELIGRMPVICTLHELSEEALVDILVKPKNAICKQYQKFFEMDGV
                     QLEFTEPALKEIARTAIKQKTGARGLRSIMEKFMLKIMFSIPDRTDIEKCIITPEVIT
                     NSQEPRYEYIKPLNSRNKVANEK"
     gene            6062..7300
                     /locus_tag="PKM80_01050"
     CDS             6062..7300
                     /locus_tag="PKM80_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNV92524.1"
                     /translation="MTSEVTIPRREKLLSTLKEKEALIIFAAEEAKLEKFKQDNNFLY
                     LTGLDIPEAIYFAFRGQNGAIEMLFIQRGDPEKEIWTGAKMTAEEASQKCGLSLQKIH
                     YLDEFLSILSFYCPVIDKIWANIGEQYLDKPPTMQLFRLSPIRERYPNIIIESIETII
                     TPLRKIKSDWEIKQLQRAIDITGKAIIDVLEKAEALMMEYELEAIMFYRMQSMGIKHW
                     GFAPIIGTGINAATLHYEKNECQIENGDVILMDVGASYLNYSADITRCFPINGSFTDR
                     QKEVYEVVLDVQKRVIEMIKPGIELLELNQVARDLLAKGAIELGLIDREEDIYKYYMH
                     SISHFLGMDTHDVGSRNAILETGNVITVEPGIYIPEEKLGIRIEDDVLVTDTGYCVLS
                     QHIPKEISEIEEIRIKAMTK"
     gene            7300..7779
                     /gene="coaD"
                     /locus_tag="PKM80_01055"
     CDS             7300..7779
                     /gene="coaD"
                     /locus_tag="PKM80_01055"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNV92525.1"
                     /translation="MSKAIYPGTFDPITLGHLNILEKACSIFDEVILAVADYTGKDNL
                     FSLQERYQLCKKSTEHIPNVSVSTFQGLFVDYAKSQNCNLMIRGMRAVSDFEYELSLA
                     LTNKKLNPEIETLFLVPSLRYMYLSSSIIRQLAALQGPLEDYVPTCVAEAMREKHFS"
     gene            7784..9160
                     /gene="dnaB"
                     /locus_tag="PKM80_01060"
     CDS             7784..9160
                     /gene="dnaB"
                     /locus_tag="PKM80_01060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNV92526.1"
                     /translation="MAKQVDAEKLNKVQTERALPADINAEAALLSAMLIDSDVVSKGI
                     EKIKEEYFYRNAHKMIFRTMEELFNEGIEIDQLTLINRLERNNFLEKVGGIPYINEIA
                     DFVVSAANFDYHLNIVTEQALLRHLIVASKGIIENCYTAAKPVKTIVDEAEQAIFAIA
                     ELPSHQGFVSFDQVSQEVITNIDKIASTKTSVTGLSSGFGDLDRFTGGFRPGQFIIIA
                     ARPAMGKTSLALNIAAHIAVNLNKKVAIFSMEMAADEVVMRMFSSASEVKMDGMLRGY
                     GMNEEKLIRIMQASEVLSSKQIFIDDSGTNSPLDIRAKTRRLSAEIGGLDLIVIDYLQ
                     LMSLVREKDNRQQEISEISRALKILAKDMKIPVIALSQLNRAAETRDNKRPKLADLRE
                     SGAIEQDADIVMFIYRDEYYFAETTTKPGIAEINIAKNRHGPTGSVELGFDQEFTLFR
                     PLETKYDQ"
     gene            9150..9899
                     /locus_tag="PKM80_01065"
     CDS             9150..9899
                     /locus_tag="PKM80_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV92527.1"
                     /translation="MISNIFAGIGEYTIFVGKCLSKITSLFKRYQEFLIQFKRIGFDS
                     LFLIFLTAAFTGLVTALQAVYQSKGYVPVNLLSVLIGKSTMIELAPVLTALVLTGKVG
                     ASIAAEIGTMRVSEQIDALESMNIDPYEFLYMPRIVAGILAFPLVTVFANAVGIVCAW
                     YFSWLHYGLHYYTFFNNMRSYFVTSDLWGGIAKSIVFGFIITSFSCYHGDRCFGGAEG
                     VGKATTLTVVYSSVFILVMDFIVAWVLFGAY"
     gene            9901..11013
                     /locus_tag="PKM80_01070"
     CDS             9901..11013
                     /locus_tag="PKM80_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV92528.1"
                     /translation="MKSWKLSLLLALLLLFSCGQEKNKQGNTHYKHKLVVYTTDEFRK
                     SGFEHSIVPDFQNKHKCQLEFVLFRNTAELCKAIKDKANYGKYDLAIGIDNSFAVSET
                     LAVNFVPPESFDSELLIRETIFDPALRLIPYAYSNLSLIYNTTQIKNPPQSFGELQDA
                     KYLAQIAICDPISSGLGRSTLFWSVALFGSEGYEYLWKSLRKNIYKTYATHTEALEAL
                     KRGECSLMIGYNTTPAFLQELDPLNKDFQVSMLKEGSYQYIESIGIHRGTSKSALCNK
                     FVDYFLASEEQKMVIYKLGMFPANRKTLLPMHFSAIPFISYSVNDRISNNLIQEQILS
                     WLDFWQRLFGYQIALQEGQISWKLESYTPSNINKES"
     gene            11168..11623
                     /locus_tag="PKM80_01075"
     CDS             11168..11623
                     /locus_tag="PKM80_01075"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNV92529.1"
                     /translation="MFHIVLFEPEIPQNTGNIGRLCVGTESTLHLIQPMRFILTDKAL
                     KRAGLDYWPNLNLQIHNSIEEIYAIFPQERIFLASTKVKKCYWDIKYQKDDVFVFGPE
                     SRGLPIELLNKYPEQGIRIPMSNKIRSINLANSVAIIMYEAVRQINCFF"
     gene            complement(11620..>13305)
                     /locus_tag="PKM80_01080"
     CDS             complement(11620..>13305)
                     /locus_tag="PKM80_01080"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNV92530.1"
                     /translation="ANMIYLQTNRNYLQLEEKLRLFFSLYDKARQGNLNYTDWKNEYQ
                     KIVTENHKLKLYRTFYQSYIQEANINYDIIMNTSLKDLRYLYRYGMFITEVNIKMAQF
                     INAYPEKELNDIACYIVQCWIDGFTRSNKNYRIKQTASIIVPCGMERLGCLIYNELDK
                     IGIKGQFNVPYKIYENKQFTYDHRFDNALIIDRDYTDMKINVYAEVVEDLKDLVSGDA
                     GPVYVELFGETPFSPVAKNTALALTDEQQELSREFMSKSAQIYYQYYRRDQGSFCIIA
                     FPSPEIGEHFPEIFADTLKLNLLDSMHYAKIQQNIIDVLDKADYVNVKGKPGNDTDIN
                     VKLHPLQNPDKETNFENCVADVNIPVGEVFTSPLLTGTNGTLHIDDIYLGSLRFYNLK
                     IQFEDGWIKGYSCTNFDDPEEGKKYIYENLLLPHKTLPIGEFAIGTNTLAYQMAKKYD
                     ILPLLPILIIEKMGPHFAIGDTCFSYEEDSPHPSFVNSKEMIAVDNEKSITRKEDPLN
                     AYTHKHIDITLPYEMLESITAVTKDGERYDIIRNGRFVVPGTEELNIPLEEGV"
BASE COUNT         4425 a         2221 c         2406 g         4253 t
ORIGIN      
        1 ccgattcgag tgttgagatt ccatttaaga gtttaagagg tgtttacagt tcaagtacag
       61 attggtcata accaggttga gatttacatt accctcaatt ttacggttga gatcttcaag
      121 aaacagtaaa aaatcgtata gacgatcatc tataccagat ttattttcta ccattgcttt
      181 cagatcatct attttatcaa tattggttat ttccgcttca aaattagcat taagagctcc
      241 taaatcactg gcaataatgc ggatatattt cagtaaatca attacctgat ctttcttttg
      301 ttgatcctta tatgcttttg ctaatcctaa atagcccaaa tcatctttgg ttgctgctaa
      361 ggcaaaaatc tcaaaagccc aattgcgcga taatgaaaca ctgtctgaag caacccgaat
      421 agcagttttt aaattgcctg cacttattcg agaagcagtt ctagcaattg gagcacttat
      481 ttcaaattct gtagtaagaa tttcttcaat aacactgcgt gtgaggggtc taaaatatat
      541 aggttgagtt cgagaaagaa ttgtaggaag aagtttttga attcgttcag taataagaat
      601 tataatagtc cgttctggag gttcttccaa tgtctttaga aatgcgttag ccgtagctat
      661 agtcatcata tctgcatctt ccacaattac aattctatat cttgcttcgt gaatggatag
      721 ttcaaaatgt ttgctaagca ttgtaatgct ttctttgcga atttcaatag tatccttgaa
      781 gaaaaaatca gcccagggag tgtttacctt attctcaatg tacttttgaa acatttttaa
      841 ggcatcagag tttttaatct caccttcagg actgaggttc aaatttggag tagggaaaat
      901 atacacaaaa tctggatgct ccagctgcag gaatttatgg caggaatcac aatgcccaca
      961 gggttttaat tcatttgttg ccatacaatt tacagccata ccaaaatata gtgctgttgt
     1021 aaatttgccc acaccatcac ttccataaaa aagataagcc tgggatattc gctcattttc
     1081 tatagcttta cgcaaaagct tgagagcgtc ttcttgtcct tttattttgt ttatcattta
     1141 cacaatacta attttactgc gacccgcttc gtcactaaca ctgtaggtat ataccttttt
     1201 aatataagga ctggtgaatt cactggcaag catatcgtaa ataatcacat catgatattg
     1261 accggataca aacataaatt ctctttgcac accaaccttt ttaaagccaa ttttttcata
     1321 acacctgata gctcgtttgt tgtaagcaag aacagaaaga tatacattat gcatattcaa
     1381 catattaaat gcaaaatcga gcaaaagatt cgtagcttca gaacctattc cttgattcca
     1441 aaaggtcttt tccccaataa aaattcctaa agaggcattc cgatgaacat cagaaacttg
     1501 atgtataccg caatttccaa tcaccttatt agtatctttt tctacaattg caaaaatgca
     1561 gttattcttc atcaattcac gcaataactc actttctttt tcgatatcta taacctgatg
     1621 agagagggtt acaaattgtc ctatttccat atcattcagc cattcagtat atttttccac
     1681 atcatccata gatacaggag aaagataaca tttctcccct acaagtttac gaaaatactt
     1741 cattttattc tccctgtgtt tatacatatt atggtattta taaacttctt atggtattga
     1801 tttcttaacc ttagcaaagt gtcaagaagt ttccgaaatt ctcgtttcac accaaaataa
     1861 agatatgatt gttccatatt ttggacaagg acaatgtctc gctaagatat gccataatta
     1921 ttaacctacc cttaatatat gtccagcaga actagttgct actcaacatc atctaatagc
     1981 atacaataaa tattgttcac aagcaacaac tcatactcct ataaatgata tcattatgat
     2041 gatctgtgca tttcttgctt gattatctac aatagagcta cagtgaagct attcgggatt
     2101 ttgccaatta atagcaccac aactccacga cagctaacta agcgatacat aactggctaa
     2161 aacagtagaa tgctgattta gtaaaaaggc aataaaaatg gtgattattt tcccagagag
     2221 gcttataaat ctgttttaat aaaaaatatc accataagtt gtcgtaataa gaaaactctc
     2281 ttgagggtga tccgtcatag gttttttgca tataaaaaac ggtataactg ctactatagt
     2341 tgtattgtcc aaagacttaa agcaatatct ctatattttt atggaaccca attaatggca
     2401 ttaaccataa aaatttatag agcataagga ggaatattgt aaacattttc tttccctcaa
     2461 ctctatattc tgtgtcaatt tcttttaggc gattcactct ttttttatta cagagacaaa
     2521 atcatctaga tttattaggt aaaacaactc attagtaagc gacatatcat acctagttct
     2581 tttctctctt atagtttgta ttactttctc ttattccaat cgaattattt tgagcagtaa
     2641 atatatgata tattacacag tacttgacag aaggcaagga tcattttttt tggatttttt
     2701 acaatgaaaa atggaagcga aagtggcgga attggcagac gcgctggact taggatccag
     2761 tgagttatac tcataggggt tcaagtcccc tctttcgcac caattttttt aatatgataa
     2821 acaggagttt ccagtgcaag tagaatttaa tcaaattaat gttgtaatca aagaaattaa
     2881 cattacaata ccttcagacg aagttacaaa agcttatgaa aaatatcttg ttaaaatagc
     2941 caaggagata gaagttcctg gttttcgtaa aggtaaagct cctctatata tggtcgagcg
     3001 ggtgaatact gaaaaaataa aagagaattt ttgttatgat tatgttattg atgtatttga
     3061 caaggttgct gaagaaaacc acatagaata tttactttat cctgatataa aagatctgca
     3121 atgggaaaaa ggcaacgata tgacggttaa gatacaaatt gaacatgaac cccagcttga
     3181 gcttaaacaa atagagggtt taaccgttcc ttataaacca gcaatactgg aagaaagtgt
     3241 agaaaaattt atcgctgatt taattgctca aaacacaata ataaaagatg ttgaaatagc
     3301 tgaagaaaat gatgaagtag aagtggaact aattgttcag aataacagtg atacttatac
     3361 tgtttctgct attttttatg cttgttcaga agataatgat tattcaatac ctgcgctaat
     3421 tgggcataaa attggagata ctctttccct tatcttatct ggtaaaaaaa ttcaaaccaa
     3481 tgcactgaat gctgatttta atcttgttga tgatactgaa tattcttgcg aactatgtat
     3541 taatcggatt agcagaaaag aaattcctgc tcttgatgat gaatttgcca aagatatgga
     3601 atacgattca ttggaagaaa tgcgggcaaa aatagctgaa gagatgaagt tgagaattga
     3661 gcatattaat atagacatag aaaataacag tataattaat aaattattta cagataaccc
     3721 tttcccttta cctatgaaaa ctgttgaata tctcgcattt caacaaatca gtgatgttag
     3781 tgatgataac ctaaggaagc ttttactaaa tcgttactat tatatgatta tgaaccaaat
     3841 ggtacaatta tatataggta atgctcttat gaaagaaaaa cctattgaat tgaccgatga
     3901 gatggttgag gaatatataa atcatcaagc tattatccaa gatgtaacgg ttgaggcata
     3961 taaagaacat agtaaaatag atttttcctc gcaggacacc cagaacagta taaaagagta
     4021 ctttattctt aggcaaattg ccaaaacctg cgagtttgtg atagaaaaag aacctccgga
     4081 aacagaaaat acagaatccg gaacagaaaa aatagaggag gaataatgag ctttgtccct
     4141 atcgtaatag aacaagttgg ccgttctgaa cgtgcttatg acatttattc ccgtttgcta
     4201 aaagatagga tcgtttttgt tgggggagtt attgaagaca atttggcaaa tactgtagtg
     4261 gctcagttac tccatctgga aggagaagat cctgaacgcg atatttatat gtatataaat
     4321 agtcccggtg gtgttatttc agctggtctg gccatttatg atactatgca atatattaaa
     4381 cctcgggtaa gtactatttg tataggtcaa gctgcaagta tggctgctgt tttacttgct
     4441 gctggtgctc ctgggaaaag aactgccctg ccaaatagta gaattatgat tcatcaacca
     4501 atgggtggag ctcagggtca agcaactgat attgaaattc aagctaaaga aatcctatat
     4561 ttacgtggac ggatgaatga aattttacac aagcatagcg gccaaactat agataaaatt
     4621 agagaagata cagaacgcaa ttattttatg agtgctgaag aggcacaaga atacggtatc
     4681 attgacgaag tgatatcgat gcgaaaatag cttgactaaa ttttaacaat cttattatca
     4741 ggtataggtg atatcaatat atcacctatg aatttttaaa atattaaaag gaggaatcct
     4801 tggagaaata cagaacatta acctgctcat tctgtggaag agcggaacat gaagtaaaaa
     4861 atttaataag aggaattgca ggtaatatct gtgacgaatg tattgttatg tgtcacacta
     4921 ttatagattc cagtaaagag gaaatttctc aggaagaatt tgatgctcct gttcccagta
     4981 gaataaaagc ttatcttgat caatatgtta tcggacaaga taatgccaaa gagattatat
     5041 cagtggcagt atataatcat tacaaacgca tattcaaacg gaaatataaa gatgatatag
     5101 atctggaaaa aagcaatatc ttgatgatag gtcctaccgg aagtgggaaa actcttattg
     5161 ctcaaacttt ggcaagattt ttacaggttc cttttgctat ttctgatgcc acaactttaa
     5221 ctgaagcagg gtatgtgggt gaagatgtag aaaatatact tgtccgctta ttacaaaatg
     5281 caaattatga tattgctaaa gcggaacgag gaattgtata tatagatgaa attgataaaa
     5341 taagccgtaa atccgaaacg ccttccatca caagagatgt tagtggtgaa ggagttcaac
     5401 aggcattatt gaagatcttg gaaggaacca aagtgaatgt ccctccaaaa ggtggacgca
     5461 aacatcctca acaggatttt attgaaatgg acacaaaaaa catcctgttc attgctggtg
     5521 gagctttttt cggtttggag aaaattatca aggaaagaat ggataaaaaa tcagtcggct
     5581 ttgatgtaga gctcggtaaa agacaggatg aagctactat ttttgattat actacccccc
     5641 aagacctttt aaagtatggt ttaattcctg aactgattgg cagaatgcct gttatttgta
     5701 ctttacatga actaagcgaa gaagctttag tagatatttt agtgaaaccc aaaaatgcaa
     5761 tctgtaaaca atatcaaaag ttctttgaaa tggatggagt ccagctggaa tttacggaac
     5821 cagctttaaa agaaattgcc agaaccgcta tcaaacaaaa aacaggagct cgcggtttgc
     5881 gttcaataat ggaaaaattt atgctaaaaa ttatgtttag tattccggat aggactgata
     5941 ttgaaaagtg tataattact cctgaggtaa taactaattc tcaggaacct cgatatgaat
     6001 atatcaagcc acttaattcg cgcaataaag ttgcgaacga aaaataacag gagaaccgat
     6061 aatgacttct gaggttacta ttccgcgccg tgaaaaatta ctttccactt taaaggaaaa
     6121 agaagcacta attatctttg cagcagagga agcaaaactg gaaaaattca agcaggataa
     6181 caacttcctg tatttaactg gtttagacat tccggaagca atctattttg ctttcagagg
     6241 acagaatgga gctatagaga tgctgtttat tcagcgtgga gatccagaaa aagaaatttg
     6301 gacaggagca aaaatgacag ctgaagaagc aagccaaaaa tgtggtctta gccttcagaa
     6361 aatacactat ctggatgagt tcttaagtat tctctctttc tactgtccag taattgataa
     6421 aatttgggct aatatcggag agcaatattt agataaacca cctacaatgc aacttttccg
     6481 tttatctcca atccgcgaac gctatcctaa tattattatt gaatccattg aaaccataat
     6541 tactcccttg cgaaaaataa agagcgactg ggaaataaaa cagttacaac gagcaatcga
     6601 tataactggt aaagctataa ttgatgttct ggagaaggca gaagccttga tgatggaata
     6661 tgaactggaa gcaataatgt tctatcgaat gcaaagtatg ggaattaaac actggggttt
     6721 tgctccaatt attggaactg gaattaatgc tgcaactttg cattatgaaa aaaatgaatg
     6781 ccagatagag aacggagatg taattctgat ggatgtggga gcttcctatt taaactacag
     6841 tgcagatatc actcgttgct ttcctataaa tgggtctttt actgatcgtc aaaaagaagt
     6901 ctatgaagta gttttggatg ttcaaaaaag ggttattgaa atgatcaaac cgggcattga
     6961 acttttagaa ctaaatcagg tagctcgcga tcttttagcg aagggagcaa ttgagcttgg
     7021 cttaatagat agagaagaag atatttataa atattatatg catagcatta gtcacttctt
     7081 aggtatggat actcatgatg taggtagccg aaatgctatt ttagaaacag gaaatgtgat
     7141 cactgttgaa ccgggaatct acattccgga agaaaaactt ggtatccgta tcgaagatga
     7201 tgttttagta acagatactg gttattgtgt tctttctcaa catatcccca aggaaatttc
     7261 tgaaatagaa gaaatccgta ttaaggcaat gacgaaatga tgtctaaagc aatttatcca
     7321 ggtacttttg atccgattac tctgggtcat ctgaatattt tggaaaaggc ctgcagtatt
     7381 tttgacgaag ttattcttgc tgtggctgat tacactggta aggataatct ttttagttta
     7441 caggaacgct accaactctg taaaaaatct acagaacata tccctaatgt atcagttagc
     7501 acttttcaag gattgtttgt agattacgct aaaagccaaa actgtaattt aatgattaga
     7561 ggaatgagag cagtatccga ttttgaatat gaactatctt tagcgttaac caataaaaaa
     7621 ctcaatcctg aaatagagac cctgtttttg gtgccttctt taaggtatat gtatctttct
     7681 tcatcaatca tccgtcagct ggctgcttta caaggtccgt tggaagatta tgttcctacc
     7741 tgtgttgctg aagctatgag ggaaaaacac tttagttaat attatggcta aacaagtcga
     7801 tgctgaaaaa ttgaacaagg tacaaaccga gagagcttta cctgctgata taaatgcaga
     7861 agcagctctt ctttcagcta tgttaataga tagcgatgtt gttagtaaag gtatcgaaaa
     7921 gatcaaagag gaatactttt accgcaatgc gcataaaatg atctttcgca caatggaaga
     7981 gcttttcaat gaaggcatcg aaatagatca attaacttta atcaaccgct tggaaaggaa
     8041 taactttctg gaaaaagttg gtggtattcc ctatattaat gaaattgccg attttgtagt
     8101 ttctgctgcc aattttgatt atcaccttaa tattgttact gagcaagcat tacttagaca
     8161 tttgattgtt gcctccaagg gaattattga aaattgctac acagctgcca aaccggttaa
     8221 aactattgtc gatgaagctg aacaggctat tttcgctata gctgaattac ccagtcatca
     8281 aggttttgtg agttttgatc aagttagcca ggaagttatt actaatatag ataaaatagc
     8341 ctctaccaaa acatctgtta caggtttaag tagtggtttt ggagatttag accgttttac
     8401 tggaggtttt agacctggtc agtttattat tattgcggct cgtcctgcta tgggaaaaac
     8461 ttctttggct ttaaatattg cagctcatat agctgtgaat ttaaacaaaa aagttgctat
     8521 tttttctatg gaaatggcag ccgatgaagt agttatgcga atgtttagtt ccgcttcgga
     8581 agttaaaatg gatggtatgt tacgaggtta cggaatgaac gaagaaaaac ttatccgcat
     8641 tatgcaggca tctgaagtgc tctcttcaaa acaaatattc attgatgatt ctggaacaaa
     8701 ctcaccatta gatatcagag caaaaacaag aagattgtca gcagaaattg gtggtctcga
     8761 tcttattgta atagattact tgcaattaat gtctttagta agggaaaaag ataaccgaca
     8821 acaggaaatc tcagaaattt cacgcgctct gaaaattctg gctaaagata tgaaaattcc
     8881 agtaatagct ctttcacaat taaaccgcgc agctgaaacc cgtgataata aaaggcctaa
     8941 attagcagac ctgagagaat cgggagctat tgaacaagat gctgatattg taatgtttat
     9001 ttatcgggat gaatattatt ttgcagaaac aactaccaaa ccgggaatag cggaaattaa
     9061 tatagcaaaa aatagacacg gacctactgg aagtgtagaa ttaggattcg atcaggaatt
     9121 tactcttttc cggcctcttg aaacaaaata tgatcagtaa tatctttgcc ggcataggtg
     9181 aatacacaat ctttgtcgga aagtgcctat ctaaaataac ttctcttttt aagcgttatc
     9241 aggaattctt gattcaattc aaacgaattg gtttcgattc gctatttctg atttttttaa
     9301 cagctgcttt tacaggactc gtaactgctt tacaggcagt atatcaatcc aaaggttatg
     9361 ttccagtaaa tctacttagt gtgttgattg gtaaatccac aatgattgaa ttagcgccag
     9421 tattaactgc tttagtttta accgggaaag ttggtgcttc cattgcagct gaaattggca
     9481 cgatgagagt aagcgaacaa attgatgctt tggaaagtat gaatattgat ccttacgaat
     9541 ttctctatat gccaagaatt gttgcaggca tcttagcctt tcctcttgtt actgtttttg
     9601 ctaatgctgt aggtatagtt tgtgcctggt atttttcttg gttacattat ggacttcatt
     9661 attacacttt tttcaataat atgcgcagtt attttgtaac ttccgatctt tggggtggca
     9721 tagccaaatc gattgttttc ggatttatta tcactagttt ttcttgttat catggagacc
     9781 gttgttttgg gggagcagaa ggagttggga aagcaacaac attaactgtt gtttattctt
     9841 ccgtttttat tctggtgatg gattttattg ttgcctgggt tctgtttgga gcatattaaa
     9901 atgaaaagtt ggaaacttag tttactgtta gctcttttgc tcttattttc atgtgggcag
     9961 gaaaaaaata agcagggaaa cacacattac aaacataaac tcgtagtcta cactaccgat
    10021 gaatttcgta aatcgggttt tgaacattct atagttcctg attttcaaaa caagcataaa
    10081 tgccagctgg aatttgtgct ttttcgtaat actgcagagc tttgcaaagc aattaaagat
    10141 aaagcaaact atggcaaata cgatcttgct attggtattg ataatagctt tgcagtttca
    10201 gaaacgctgg ctgttaattt tgtgcctcca gaatcttttg attcagaatt gctaattcgg
    10261 gaaactatct ttgatcccgc gttaagatta ataccttatg cttatagtaa tctttccctg
    10321 atatataata ctacccagat aaaaaatcct cctcaatcat ttggagaatt gcaggacgcc
    10381 aaatatcttg ctcaaattgc tatctgcgat cctatcagtt caggtttggg acgctcaacc
    10441 cttttttgga gtgtagcact ttttggatct gagggctatg aatatttatg gaagagctta
    10501 cgcaagaata tatacaaaac ttatgctact catacagaag ctttagaagc acttaaaaga
    10561 ggggaatgtt cattaatgat tggatacaat accactcctg cctttttaca ggaacttgat
    10621 cccttaaaca aagatttcca ggtttctatg ctaaaagagg gtagttatca atatatagaa
    10681 tcaattggta ttcatcgtgg aacttcaaaa tccgctctat gcaataagtt tgttgattat
    10741 tttctggctt ccgaagaaca aaaaatggta atttataagc tgggaatgtt ccctgccaat
    10801 agaaagactc ttctgcccat gcatttttca gctataccct ttataagcta tagtgtcaac
    10861 gatcgcatat ccaataattt gattcaagaa caaattttgt catggctgga tttttggcaa
    10921 aggttatttg gttaccaaat agctttgcaa gaagggcaaa tatcctggaa attagaaagt
    10981 tatacacctt ctaatataaa caaagagtca taaacgcaat aattaattag cggggactta
    11041 agatttcttt atataagcat agtgcactca ctacataaat ttaggtaggt attgccaatg
    11101 ggagttatat ataaagatat cgtttatttt gctgaggatg agcatcccta aatttctcga
    11161 ggtaaaaatg tttcatattg ttttatttga acctgaaata ccacaaaata caggaaatat
    11221 aggccgtctt tgtgtaggta cagaatctac tctacattta attcaaccaa tgcgttttat
    11281 cttaacggat aaagcactaa aaagagcagg attggattat tggccaaacc taaatctgca
    11341 aattcataac agcattgaag aaatctatgc tatatttcct caggaacgaa tcttcttagc
    11401 aagcaccaaa gtaaaaaaat gttattggga tattaaatac caaaaagacg atgtttttgt
    11461 atttggacct gaatccagag gtttacctat agagctttta aataaatatc ccgaacaagg
    11521 aatccgcata cctatgagta acaaaattcg cagtataaat cttgctaatt cagtagctat
    11581 tattatgtat gaagcagtaa ggcaaattaa ctgttttttt taaacacctt cttccagagg
    11641 gatattcaat tcttctgttc cgggaacaac aaatctgcca tttctaataa tatcgtatct
    11701 ttccccatct ttagttacag cagttataga ttccagcatt tcatagggca gggttatatc
    11761 aatatgttta tgagtatatg catttagcgg atcttcttta cgagtaatag atttttcgtt
    11821 atctacagca atcatctctt tagagtttac aaaactggga tgggggctat cttcttcata
    11881 actgaaacag gtgtcaccaa tggcaaaatg aggccccatt ttttctatta tcaatatggg
    11941 cagtaaaggt aggatatcat atttcttagc catctgataa gccaaagtgt ttgttccaat
    12001 agcaaattct ccaatcggaa gagtcttatg aggcagcaac aaattttcgt atatatactt
    12061 cttgccttct tccggatcat caaaattcgt acaggaataa cctttaatcc agccatcttc
    12121 aaattgaatt ttcaaattat aaaagcgcaa agaaccaaga taaatatcat ctatatgtag
    12181 tgttccatta gtgcctgtta ataggggaga agtaaaaacc tcaccaacag ggatattaac
    12241 atcggctaca caattttcaa agttggtttc tttatcagga ttttgcagag gatgcaattt
    12301 aacattgata tcggtatcat ttcccggttt gcctttcaca tttacataat cagctttatc
    12361 caacacatca attatattct gctgaatttt ggcatagtgc atactatcca gaaggtttaa
    12421 ttttaatgta tcagcaaaaa tttctgggaa gtgttctcca atttcaggtg aagggaaagc
    12481 gataatgcaa aaactgcctt gatctcttct ataatattga taataaattt gagcggattt
    12541 agacataaat tctctggaaa gttcctgttg ctcatcagtt aaagctaaag cagtgttttt
    12601 tgccacaggt gaaaaaggtg tctctccaaa aagttctaca taaacaggtc ctgcatcacc
    12661 gctaaccaag tctttcaggt cttcaactac ttcagcatag acatttattt tcatatctgt
    12721 ataatcccta tctataataa gcgcattatc aaaacggtga tcgtaagtaa actgtttgtt
    12781 ttcataaatc ttataaggga cattaaattg tccttttatc cctattttat ccagttcgtt
    12841 ataaatcaaa caccccaatc tttccattcc gcaaggaaca ataatacttg ctgtttgctt
    12901 aatccgataa tttttgttgg agcgggtaaa accatcaatc cagcactgaa caatataaca
    12961 ggcaatatca tttaattctt tttccggata ggcattgata aattgagcca ttttgatgtt
    13021 aacttcagta ataaacattc cataacgata gagataacgc aaatctttta gactagtatt
    13081 cattattata tcgtagttta tgttcgcttc ctgaatgtaa ctctgataaa atgtccgata
    13141 gagttttaat ttatggtttt ctgtcacgat tttctggtat tcatttttcc aatccgtata
    13201 gtttaaattt ccttgcctcg ctttatcgta taaactaaaa aacaagcgca atttctcttc
    13261 taattgcaag taatttctat ttgtttgtag atagatcata ttagc
//
LOCUS       DAORHB010000017        13219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3763_length_13219_cov_4.887040, whole genome shotgun sequence.
ACCESSION   DAORHB010000017 DAORHB010000000
VERSION     DAORHB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13219
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3763_length_13219_cov_4.887040"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="PKM80_01085"
     CDS             <1..1669
                     /locus_tag="PKM80_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92531.1"
                     /translation="LVVVFTLFTIPFFAQKITTAEPTKVDTLELVEKKVTKAEIENWI
                     KANHLDTNEHIFGASPLHIDYGDIYHNGYFYVGRSEMNMPERNGFETLSGIFSPALYY
                     GYFNSLYQVKEYQRTVNYTNNSYPFPPVLTSLKAGLGDYDHHYARINLSKNELFSYPY
                     LEYQGDFLVQNGYWTDITSTETSMKHRLSQSGKLVTWELEFANWKKDIAMNELLPVYW
                     QSTNFTINHNLQQFYASFALPWFKLKLLQESESANTNLFYKEYNRNSTRLQFSAQQKI
                     GFLQTKELYEHNWNKGFSCFCGFYNAEEYQDKLGFGFQINNPVKINLTADCLDWKRYR
                     LFANADYSLKNFNLGATHEHKMGDDSSVLKVKDIFNADGELDLIDISFSQESSVYCSY
                     NLFNIKPLVAVGTREIEQEAENSWLNIKKQQPFLRLAMDVNYSWKKWLITARPEWVIS
                     KYYPSLTENPEYRFHSVQQIKYILPYDNAVVVGCDIYGHSEYYVANALNPYLIESSTM
                     LDAWAGINVGNLFELRTGFKNVLSSSLYGTYPVPVSVYIDVLWLYLN"
     gene            1932..2735
                     /locus_tag="PKM80_01090"
     CDS             1932..2735
                     /locus_tag="PKM80_01090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNV92532.1"
                     /translation="MKLFETHCHLDHPDFDSDRESLINKCFSSGIEYIINVAFNKETA
                     FSSLGLAKKHLHIFSTVGYHPHDAIDFDAEIVKKLAREKKVVAIGEIGLDFFRNLSPF
                     TVQREVFANQAHLAVEYDLPVIVHDRDAHQECYNILKNENIKEAVFHCFSGDIIFAQQ
                     VLDAGWMISFTGNITYANIHLDDVIRLVPLDRFMVETDCPYLPPHPHRGERNSPLYLP
                     LVVEKIAEVKGISPKEVAELSFANAYRFFRIPPDPVKALHHKSSHKKVN"
     gene            2783..3988
                     /locus_tag="PKM80_01095"
     CDS             2783..3988
                     /locus_tag="PKM80_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92533.1"
                     /translation="MKKAVIIICGAILCISLQAGNSIFSFYGFPVHNYGRDIYSLGMG
                     DTGASDVFRINTGYANPAMHNRNNYTLFSTGMNMGYTKYHSQYEGIERTFRDDSLDFP
                     YFNINIPIGRNRLGVQFNSLASGLVTNYHYLPDGSVESQTSDKYLYKADLIYSYSFSE
                     VNLGISANYYFGHNKQYFSQTGNYSNFDTRESIIMDFKNPSITVGTIKLFDKYSVGAH
                     YCAPVTLKGELIRSSIHNTEPAEDYKFKLPEQYCLSATALPFEQFKVAADINYEPWST
                     ISDDYRDCYKIGLGLAYEPNMEAHKNIFMRFPMRVGASMRQLYFRDKDGKDIDEVSLS
                     CGLTLPLKNEIDRIDLGFQYLKRGDLATNNLSENSYMLMIGFTGFDIISKAPDRTAPR
                     NIPVKEEVE"
     gene            3985..5541
                     /locus_tag="PKM80_01100"
     CDS             3985..5541
                     /locus_tag="PKM80_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNV92534.1"
                     /translation="MKPDEPNSNMDSSNDILNQILKSRNLSQQDLQTNLNMLPDEALF
                     ANIDKVEHRIRAALYENEPMVIFGHDDPDGITSTYILYNFLNSCGYQKHRYYIPNRNL
                     EPHGIQAGFIDFVREGNYKLIVTVDNGIASLEGVKQLNALGCDVIITDHHIVQPEVLP
                     PAYAIMNPQLPQCDYPFKQLAGVGVVLMLIRYLGNKWEHPIEPASYFWTAVGSVADKV
                     PMIGMNRILVRYALEHFYEIQDDTVEFLLRNYNRINTLSDINSFLQYTAHLLANGRDD
                     KGRHTALRFILQLSDAKADLFQELENQKNKWDSDLNRVFNFLDTLAADFVGNWFVYYD
                     DEDIIPYPLLGTAATYILNKLNIPTLLLKSHNGDTVCEGRCSEGFNIMDAFTHCKKYL
                     KQFGGHPKAAGFTMQQENYDTFLDCFNNFLLKHFHPSHTASTKYDAVVAAKDINRENW
                     QKLEILLPWGQLNPEPTLLIRNTSKAEIMNYVALDNSGREIPNQGKGDILALWKAANM
                     IKVLSWQQTD"
     gene            5570..6157
                     /locus_tag="PKM80_01105"
     CDS             5570..6157
                     /locus_tag="PKM80_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HNV92535.1"
                     /translation="MIQLGETLRKSIHLSSLIIPLGYRYLLSFNRSLGFSILLIAFTI
                     SLVIEFYRFWQKGFRKTFHRIFGIILRKHEFKDFTGATYMLFAAVLCVAFFPPLISSC
                     AMAFLTIGDTFAALVGINFGKRWYIKQNKSLEGSLACFVSCSVFGIFWLGSPFLAVLG
                     ALAATLAELSNIPIDDNITIPIFSALVMTLLSIVI"
     gene            6169..7152
                     /locus_tag="PKM80_01110"
     CDS             6169..7152
                     /locus_tag="PKM80_01110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV92536.1"
                     /translation="MKLSFVIPVLNEQDSLVQLYSEIIQNCNGYDYEIIFIDDGSTDN
                     SFNIMSQIALSDMKVKVVQFRCNFGKAAALQTGFNLTSGDIVFTLDGDLQDNPIEIPN
                     FIAKLDEGYDLVSGWKRKRRDPIYKTLPSKLFNFVTAKTFHLKLRDYNCGFKAYRHPL
                     EKEIILYGEMHRYIPVLAHSLGYKVGEIPVAHRPRKFGKSKFGKERYLRGFFDLLTVK
                     MITQYSKSPLYLFGRIGIASTFLGLIISIYLAILKIFMNQPLSNRPLLFLGILLILGG
                     LQFISMGLISELIVNRFRQGNRTPVSIAQYVNIDQIESKPYQQTNLPEVNE"
     gene            7145..8368
                     /locus_tag="PKM80_01115"
     CDS             7145..8368
                     /locus_tag="PKM80_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV92537.1"
                     /translation="MSKLELFFLSRYLKAPKRNLLRFSFVFMILGIILSVGILSAGLN
                     LFEGYERTLKDVLLGAFPHLTISKDNLGFISHSETKNLLNKMANCKEIQQVTPVLSYP
                     VMAAGKAKVRGATLNAYDFSNKKSFPQAKYIQKGKKIPASQEVIIGKYLAKELGKDIG
                     DTLKLVFTRLDNISALGIFPSEYYITVVGIYSSGFYETDRSLILANITDAENMLNSAS
                     GFSKIEIRLDPKYIDKAPEIGQKLQEIVGNYYSVYPWQTFSAGLLHLVTMEKWLIFII
                     FCFLVLIAGINVISAVSTIILDKKNEIAVLKTLGANSTSIKRVLSFQVGLSALLAILA
                     GQILGALLSWGIEKQNFYKLKGDVYFIDSLNTSIAPLNQLIIFIVSAALVFICIYYPL
                     KQIDKLEIIELLRNP"
     gene            8421..9092
                     /locus_tag="PKM80_01120"
     CDS             8421..9092
                     /locus_tag="PKM80_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92538.1"
                     /translation="MVCLAGYDLCKSYLDSNQTINVLRKATIEVKEAELVCITGQSGS
                     GKSTLLHLLGLLDSPDSGKVMISGREINSTMPEAASIRNLELGFVFQFHYLIEDLNAQ
                     ENVALPMIISGVNSSQAIKKAAELLQALDLEDRINHYPNQLSGGEQQRVALARALINN
                     PKIVLADEPTGNLDPEHSAEVWDMIIKLNKERGQTFVIVTHDIVNASKAKVIYNLSEG
                     KLEKK"
     gene            9105..13169
                     /locus_tag="PKM80_01125"
     CDS             9105..13169
                     /locus_tag="PKM80_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92539.1"
                     /translation="MPRSKKFLIFVLILLLINTGFFLAWYAFGLNNFFKNSVAKYIGQ
                     LTKGKVTIEELHFSDRQLLAKGVNYTSADSSLAISINRLSARYNLTRYLLSGFKTNKL
                     VNDIEIYKPVVHYSYQYKPQPPKAKKPLVLPNFAKYFKHLQLNNGAAVLSFNIPVKIT
                     QEGNLTISESLSNINLEADNKSTTNATINALTSNGGKISAKGLLDKGRIVSVDGEISA
                     YKPLFVSHPDLQNLHTEISLVGSLKQDSLAAPLKYNAEAQIWGTETLFAKNYPVLIPY
                     LWVKTDGNNLKLSLSKSNVGSSDLAAEVAISNLRHKIKFERVDISADVDLAMIQKDLK
                     GMVNANIIGEGTIDKPELELSLVSEQAAYQNYTFNKFNFSGTYQNDLLDFIIPELIFG
                     NQIINLTGSFNPLNMILDANLETKPINKKAQPYLATAKLDLYAELWGKYPYVDATIHQ
                     LDFAYELANIENVSGKIKLLPLPDVNDLYLDANLSSSNGFNIEVIGDVLDRNLLVDAK
                     FDDLSIAELYAQPTLVNLNPGLKGELKAIITGDNIVVQTLTGLTLDNPVPVDLTLDAL
                     GTINYKMKDASIYVSGTEGIVNNQPLNFELAAMLKDNIVTLQGLKVNDLINVSGAINL
                     ANYADMNIGLNINNLNYLDVVRYYPKLDVNLPEFNGLNVFLEYNRDNLQRLRANVFLS
                     DIDLLAVTPFSLELGMEGPLNNILVFSTIKNKEQNIVDMTGYVSLTPELNVNLDAAFK
                     DLHIQDVLINSPIYGIIDGISGITWHKVKEQKPELELRADLISRDIVLGNYTIDKAII
                     KGKQTPRKMIVDSLFVKSTGLFELSGSGAINYNALYNEFFEGPEHLNLRAQGELFTWL
                     NGLTPYIQEAKGSSSLSASIGTTEEQFMVSTGKINISGGYIQIKDQTEPCSNINIRGG
                     FDKNRVVIDYGQIQMGEGKLVFNNIFEVDNSDHIMLGFLDLGILRLMVEEPGIMINVP
                     KYTTPRSLTKVILKGNNSRYATIKGPFDQMKISGEVIASNTNVLYPPDTENLLTLATS
                     MRGTNKKSSSEPSPLPFTLDVKLIAGENVKYVTYPAKLRVVPGGYLHLMYDGLSFIAE
                     EASFSSEQGTVDIFGTIFQVDKVNLTLVDSQNLLGLEGIFFKRAPDGSMITLTAVTSN
                     DLTKPFMERLQLNLASDNPEDKTISQILARLRYSGSETPDQKQGDILQDEALTLISGN
                     LDASLFTPILSPVENYIRRTLHLDSFTINAGFLQNLYTEYTSNPKQFAEYTDMNELSS
                     DIAKFSSSILLNNLSISMSKYLGSSLFLDYMLTLQEATNLQKRTRILVSNEVSIRLIL
                     PDNYRVSYTLKNEAQEKKTSHEIMLSRSYRFWGL"
BASE COUNT         4246 a         2213 c         2431 g         4329 t
ORIGIN      
        1 gttggttgta gtttttaccc tttttactat tccttttttt gcccagaaaa taacaactgc
       61 agagccaact aaagttgata ctttagagct tgtagagaaa aaagttacta aagctgaaat
      121 tgaaaattgg ataaaagcaa atcacctcga cactaatgaa catattttcg gtgcatcacc
      181 tttacatata gactacggag atatatatca taatggctat ttttatgtag gacgctctga
      241 aatgaatatg ccagaaagaa atggttttga aactttatcc ggtatttttt ctcctgctct
      301 ctattatggg tatttcaatt ccttatatca ggttaaagaa tatcagcgca cagtaaatta
      361 tacaaacaac agttatcctt ttccgcctgt tctaacatct ttgaaagcag gattgggaga
      421 ttatgatcat cattatgcgc gcatcaattt atctaaaaat gagctttttt cttatcccta
      481 tcttgaatat cagggtgatt ttctggttca aaatggttat tggacagata ttacttctac
      541 tgaaacttct atgaaacatc gcctttccca atcaggaaaa ttagttactt gggagctcga
      601 atttgccaat tggaaaaaag atattgctat gaatgaactg cttcctgtat actggcaatc
      661 tacgaatttc acaatcaacc ataacttaca gcagttttat gcctcatttg ctttaccttg
      721 gtttaaacta aaattactac aggaatccga aagcgcaaat actaatctgt tttacaaaga
      781 gtataatcgt aattccactc gtttacaatt ttctgctcag caaaaaattg gctttctgca
      841 aactaaagaa ctatatgaac ataactggaa taaaggtttt tcttgttttt gcggatttta
      901 taatgctgaa gaataccaag ataagctcgg gtttggtttc cagataaaca atccagtaaa
      961 aatcaatctt accgcggact gcttagattg gaaacgctac aggttatttg ctaatgctga
     1021 ttattctctt aagaacttta atttgggagc aacccatgaa cacaaaatgg gtgatgattc
     1081 ctctgttttg aaagtgaaag atatttttaa tgctgacgga gaattggatt taatagatat
     1141 ctctttttcg caggagtcat cggtttattg tagctataac ctcttcaata ttaagccatt
     1201 agtggcagta gggacaagag agattgagca ggaagcagaa aattcctggt taaacataaa
     1261 aaaacagcaa ccttttctcc gcctggcgat ggatgtgaat tatagctgga aaaaatggtt
     1321 gataaccgca agaccggaat gggttatcag taaatattat ccatcgctta cagaaaatcc
     1381 ggaataccgc tttcatagtg ttcaacagat aaaatatata ttaccctatg ataatgcagt
     1441 tgtagtaggt tgtgatattt atggccattc agaatattat gtagccaacg cgctaaaccc
     1501 atatttgatt gaaagttcca caatgctgga tgcttgggca ggaattaatg taggaaattt
     1561 atttgaatta cgcactggat ttaaaaatgt tctttcctct tctctatatg gaacatatcc
     1621 agttccagtt tctgtatata tagatgtttt atggctctat ttgaattaag aatagataac
     1681 ggataactgg atcgattgga ttaacaaggt ttatttcaat agaagattta atctgtatga
     1741 actatttttt aatctgagta atcaagataa tctgtgagag gggttatata tatgaaagtt
     1801 acgagtttaa tatctatcaa tgttttgatg ttcttaatga agaataaata gcataataga
     1861 acctgcttga caaaaaaagg actcttatat tatattgaat tcatcctaaa ataggggtta
     1921 aataattaaa aatgaaatta tttgaaacac attgtcacct ggatcatcct gattttgaca
     1981 gcgatcgaga aagcttaata aataaatgct ttagcagtgg aatagaatac attattaatg
     2041 ttgctttcaa caaagaaacg gcgttcagtt ctttggggct ggccaaaaag cacttacata
     2101 tcttttccac agttggttac caccctcatg atgctattga ttttgatgct gagatagtaa
     2161 aaaaactggc aagagagaaa aaggtagtag ccattggtga aatcggattg gactttttcc
     2221 gaaacttatc tccatttaca gtgcagcgtg aagtttttgc caatcaagca catttagctg
     2281 tagaatatga tttaccggtt attgttcatg atcgagatgc tcatcaggaa tgttataaca
     2341 ttttaaaaaa tgagaatatt aaggaagcgg tttttcattg tttctccggt gatattattt
     2401 ttgcgcaaca ggttttggat gcaggttgga tgatttcttt tacaggaaat atcacctatg
     2461 ctaatattca cttagatgat gtaattagat tagttccctt ggatagattt atggtagaaa
     2521 cggattgtcc ctatttacct cctcacccgc atagaggtga aagaaattcc ccactatatt
     2581 tacccttagt agtagaaaaa atagctgaag taaaagggat ttctcctaag gaagtagcag
     2641 aattatcatt tgccaatgct tatagatttt tccgtattcc tcccgatcca gttaaggctt
     2701 tacatcacaa atctagtcat aaaaaggtta attaaaactt taagctgcaa aaagatatgg
     2761 ctttaaagtt aaaggatata gaatgaaaaa agcagttatt attatctgcg gagcaatctt
     2821 atgtatatct ctccaggcag gaaactccat attttcgttt tacggttttc ccgttcataa
     2881 ttatggtaga gatatttaca gtttaggaat gggcgataca ggagccagtg atgttttcag
     2941 aatcaatact ggttatgcca atccagctat gcacaacaga aataactaca ctttattttc
     3001 cactggaatg aatatgggtt atacaaaata tcactcacaa tatgagggaa tagaaagaac
     3061 ttttagagat gattctttag attttcctta ttttaatatt aatattccaa ttgggagaaa
     3121 taggttaggt gttcaattca attctcttgc ctctggatta gtaaccaatt atcactatct
     3181 tcccgacggg tcggttgaat ctcaaaccag cgataaatat ctttataagg cagatttgat
     3241 ttacagttac agttttagtg aagtgaacct gggaattagt gccaactact atttcgggca
     3301 taacaaacag tatttttcac aaactggaaa ctactccaat tttgacactc gtgaatcaat
     3361 aataatggat tttaagaacc ccagcataac cgttggaact atcaaattat ttgataaata
     3421 tagtgttgga gctcattatt gtgcacctgt tactttgaaa ggtgagctga ttcgtagcag
     3481 catacacaat actgaacctg cagaagatta taaatttaaa cttcctgaac aatattgtct
     3541 aagcgctaca gccttgccgt ttgaacaatt caaggtggct gctgatatta attatgaacc
     3601 ctggagcaca atatcagacg actatcgtga ttgttataag attgggctag gacttgctta
     3661 cgagccaaat atggaagcac ataaaaatat ttttatgcgt tttccgatga gggtgggtgc
     3721 ttctatgcgt caattatatt ttcgcgataa agatggcaaa gatattgatg aagtatcact
     3781 tagttgtgga ttgactcttc ctttaaaaaa tgaaattgat agaattgatt taggatttca
     3841 atatctgaaa cgcggtgatt tagccaccaa taatctcagc gaaaattctt atatgttgat
     3901 gattggattt actggttttg atattatcag taaagctccc gatagaacag ctccgcgtaa
     3961 tatacctgta aaagaggaag ttgaatgaaa ccagatgaac ctaatagtaa tatggattct
     4021 tctaatgata tattaaatca aatactaaaa tctcgcaatt tatcgcagca ggatttacaa
     4081 actaatctta atatgcttcc tgatgaagct ctttttgcaa atatagataa agttgaacac
     4141 aggattcggg cagcactcta tgagaatgaa cctatggtaa tttttggcca tgatgatcct
     4201 gatggtataa ctagtactta tattttatat aactttctta attcttgtgg ttaccaaaaa
     4261 catcgttatt atattcccaa tcgcaatctg gagccgcatg gcattcaagc aggattcata
     4321 gattttgtgc gcgaaggtaa ttataagctt atagttacag tagataacgg tattgcatct
     4381 ttggaaggag ttaaacagct taatgcctta ggatgtgatg ttataataac tgatcatcat
     4441 atcgtgcaac cagaggttct tccaccagct tatgcaatta tgaatcctca gcttcctcaa
     4501 tgtgattatc ctttcaaaca acttgctgga gttggagttg ttttaatgtt aattcgttat
     4561 ttgggaaaca aatgggagca tcccattgag ccagcaagtt atttttggac ggctgttggt
     4621 tctgttgccg acaaagttcc tatgatagga atgaatagaa tattagtccg ttatgcatta
     4681 gagcattttt atgaaattca ggatgacaca gtggaattct tattgcgtaa ttacaatcgc
     4741 ataaacacat taagcgatat taacagtttt ctgcaatata cagctcatct tttagcaaat
     4801 gggcgtgatg ataaaggaag gcatactgct ttgcgtttta ttctacaact ttcagatgca
     4861 aaagcagatc tttttcagga gctggaaaac caaaaaaaca aatgggatag cgatttaaac
     4921 cgagtattca attttttgga taccttagct gctgattttg taggcaactg gtttgtatat
     4981 tatgatgatg aagatattat tccctatccc cttttaggta cagcagctac ttatatttta
     5041 aacaaactga atattcccac tttattgctt aaaagccata atggcgatac tgtttgtgaa
     5101 ggtagatgca gtgaaggttt taatattatg gatgccttca cccactgtaa aaagtactta
     5161 aagcaatttg gaggtcatcc taaagcagca ggttttacga tgcaacaaga aaattatgat
     5221 actttcttgg attgttttaa taatttcctc ctgaaacact ttcatccttc ccatacggca
     5281 tctacgaaat atgatgctgt ggtagcagcc aaagatatta atcgggaaaa ttggcagaaa
     5341 ctggaaattt tactaccctg gggacagttg aatccggagc caactttgtt aattcgtaat
     5401 acttctaaag ctgaaataat gaattatgtt gctctggata atagtggacg ggaaattccc
     5461 aaccagggca aaggcgatat tttggctcta tggaaagcag ctaatatgat taaagtttta
     5521 tcctggcagc aaacagacta atgatcaagc ttgctggagg cacagacatt tgatacaact
     5581 tggtgaaacc cttaggaaat ctattcattt aagcagttta attattccgc tgggatatag
     5641 atatctgctt tccttcaatc gttctttggg cttctcgatt ttgttgattg ctttcactat
     5701 cagtttagta atagaatttt accgtttctg gcaaaaggga tttcgtaaaa cctttcaccg
     5761 catatttggc atcatattaa gaaagcacga atttaaggat tttacaggag caacttatat
     5821 gctctttgcg gcagtgttgt gtgttgcttt tttccctcct ttgatatctt cctgcgcaat
     5881 ggctttttta acaataggtg atacttttgc tgcattggta ggtataaatt ttggtaaacg
     5941 ctggtatatt aagcagaata aaagtttgga aggtagtttg gcctgttttg ttagctgttc
     6001 tgtctttggt attttttggt tgggaagtcc ttttttagct gttttgggtg ccttagccgc
     6061 aactttggct gagctaagta atattcccat agacgataat ataacaattc ccattttttc
     6121 agctttggtg atgactttgc tgagtattgt aatataaaaa aggtagctat gaaattatcc
     6181 tttgttattc cggttttaaa cgaacaggat tctcttgttc agctatattc tgaaattata
     6241 caaaactgta atggctatga ttatgaaatt atctttattg atgatggctc aacagataac
     6301 agttttaaca ttatgagcca gatagcttta agcgatatga aagtgaaggt tgtacaattc
     6361 agatgtaatt ttggcaaagc tgctgcttta caaactggtt ttaatcttac ttcaggagat
     6421 atcgttttta ctttggatgg tgatcttcag gataatccta ttgaaattcc caattttata
     6481 gccaaattgg atgagggcta cgatctggtt tcaggttgga aacgcaaaag acgcgatcct
     6541 atttataaaa ctctcccatc caaacttttt aatttcgtaa ccgcaaagac ctttcatctt
     6601 aagctaagag actataattg tggttttaaa gcatatcgtc atcctttgga aaaggagatt
     6661 atactttacg gtgagatgca tcgttacatt ccagttttag ctcattcttt aggatataaa
     6721 gttggcgaaa ttccggttgc tcatcgtccg cgtaaatttg gcaagagtaa atttggaaaa
     6781 gagcgttatc tgcgtggttt ttttgatctt ttaactgtta aaatgattac ccaatatagc
     6841 aaaagtcctt tatatttatt tggacggatc ggaattgctt ccacctttct gggtttgatt
     6901 atcagtatct atctggcaat tctgaaaatc tttatgaatc aacctctctc caatagaccc
     6961 cttttgttct tgggaatttt gcttatcttg ggtgggttgc aatttatctc tatgggccta
     7021 atttctgaac ttatagtaaa tcgtttccga cagggaaacc ggacacctgt ttctatcgct
     7081 caatatgtaa atatagacca aattgaatcc aaaccctatc aacaaactaa cttacccgaa
     7141 gtaaatgagt aagctggaac tctttttttt aagtcgttat ctgaaagctc ccaaacgtaa
     7201 cctattacga tttagttttg tttttatgat tttggggatc attctctccg tgggaatcct
     7261 aagtgccgga cttaacttat ttgaaggtta tgaacggact ttgaaagatg ttttattggg
     7321 agcttttccg catctgacaa taagtaaaga taatctgggt ttcatctccc attccgaaac
     7381 aaagaaccta ctgaataaaa tggctaattg taaagaaatt cagcaagtta ctcctgtcct
     7441 tagctatcct gttatggctg caggtaaagc aaaagtgaga ggagccactt tgaatgctta
     7501 tgattttagc aataaaaaat ctttccctca ggcaaagtac attcaaaaag gtaaaaaaat
     7561 accagcttct caagaagtta tcattggcaa atatctggca aaggaattag gaaaggatat
     7621 tggcgatact ttaaagttag tatttactcg tttggataat atatctgcat taggcatttt
     7681 tccttcggaa tactatataa ccgttgtagg tatttacagc agtggatttt atgaaacaga
     7741 tcgctcttta attcttgcca atattaccga tgcagaaaat atgcttaatt cagcatcggg
     7801 cttcagtaaa attgaaattc gtttggatcc taaatatata gataaagcac cggaaattgg
     7861 gcaaaagctt caagaaatag ttggtaatta ctattcagta tatccttggc aaacctttag
     7921 tgcaggttta ttacatttgg taactatgga aaagtggcta atttttatta ttttctgttt
     7981 tttagtttta attgctggaa ttaatgttat atctgcggtg tcaactatca ttctggataa
     8041 aaaaaatgaa atcgccgtgc ttaaaacctt gggtgccaat tctacatcta taaaaagagt
     8101 ccttagtttc caggttggac tttctgctct gctagctatt ttggcaggac aaattttggg
     8161 agctcttctt tcttggggta tagaaaagca gaacttttat aaactaaagg gtgatgttta
     8221 ttttattgac agcttaaata caagcatagc acccttaaat cagttgatca tctttattgt
     8281 ttcagcagct ctggttttta tttgtatata ctaccccctt aagcagatag ataagctgga
     8341 aattattgaa ttgttacgca acccctgatg gattataata taatataaca ctaactaatt
     8401 tttataaaaa ggattcgaaa atggtctgtt tagcaggtta tgatctctgc aaaagttacc
     8461 ttgatagtaa tcaaacaatt aatgtgctta gaaaagccac aattgaagta aaagaagccg
     8521 aactggtttg tattacagga caatctggct ccggaaaaag cactctatta catcttttag
     8581 gtttattgga ttccccagat tcaggaaaag tgatgataag cgggcgcgag ataaattcca
     8641 ctatgccaga agcagcttca attcgtaatt tagaactcgg ttttgttttt caatttcact
     8701 atctgataga agatctaaat gcccaagaaa atgtagcttt accgatgatt atttctggcg
     8761 taaatagttc ccaagcaata aaaaaagctg cagaactttt acaagctcta gatttagaag
     8821 atcgtatcaa ccattatccc aatcagttaa gtggcggtga acagcaacgg gttgctcttg
     8881 ctagagcttt gataaacaat cccaaaattg ttttggcaga tgagcctaca ggaaatctcg
     8941 atcctgaaca tagtgccgag gtttgggata tgattattaa attgaataaa gagcgcggtc
     9001 agacctttgt gattgtaact catgatattg ttaatgcctc taaagcaaaa gtaatctata
     9061 atttaagtga aggcaaactg gaaaaaaaat aggcaagatt aagtatgcca agatcaaaga
     9121 aattcctgat attcgtttta attctgttgt tgataaacac aggattcttt cttgcttggt
     9181 atgcatttgg tttaaataat tttttcaaaa actctgtggc taagtatatc gggcaactta
     9241 ccaaaggcaa ggttactatt gaagagttgc attttagcga tcgtcaatta ttggctaaag
     9301 gagtaaatta tacttctgct gatagcagtt tagctataag cattaatcgt ttaagcgcac
     9361 gctataatct aactcgatat cttctctccg gtttcaaaac caataagttg gttaatgaca
     9421 tagaaatata caagccagtt gtacactata gttaccaata taagccccaa cccccgaaag
     9481 caaaaaaacc gcttgtcctt ccgaattttg caaagtactt taaacatctt cagcttaata
     9541 atggagctgc agttttgtcc tttaatatcc ctgttaagat tacacaagag ggcaatttaa
     9601 ctatatcaga gagtttatcc aatataaatc ttgaagctga taataaatcc acaacgaatg
     9661 ctacaattaa tgccctcact tccaatggtg gaaaaatatc tgctaagggt ttactggata
     9721 aaggaagaat agtttctgta gatggagaaa tatcagcata taaacctctg tttgtctctc
     9781 atcctgattt gcaaaatttg cacacagaaa tatcgttggt tggttcttta aaacaggatt
     9841 ctttagcagc acctctgaaa tacaatgcag aagctcagat ttggggaaca gaaacactat
     9901 ttgccaaaaa ttatccagtc cttattccct atttatgggt taaaacagat ggtaacaatt
     9961 taaaattatc cctttccaaa agcaatgttg gcagcagcga tcttgctgca gaagttgcaa
    10021 tcagcaattt aaggcataag ataaaatttg aacgggtaga tatttcggct gatgtagatt
    10081 tggcaatgat ccaaaaagac cttaagggaa tggtgaatgc taatattata ggagagggta
    10141 caattgataa accagaattg gagcttagct tagtttctga acaagctgct taccaaaatt
    10201 acacttttaa taaatttaat tttagtggaa cttatcaaaa cgacttgctg gattttatca
    10261 ttcctgagct tatttttggt aatcagataa ttaacttaac aggtagtttt aatccgctaa
    10321 atatgatatt ggatgctaat ctggaaacta aacctatcaa taaaaaagct caaccttatt
    10381 tagcaactgc caaattggat ttatatgccg aattatgggg taaatatcct tatgtggatg
    10441 caactatcca ccagcttgat tttgcttacg agcttgctaa tattgaaaat gtaagtggaa
    10501 aaataaaact attgcctctt cctgatgtca acgatctata tttagatgcc aatttatcaa
    10561 gtagtaatgg atttaatatt gaagtaatag gagatgttct tgatcgtaat ctcttagtag
    10621 atgccaaatt tgatgatctt tctattgcag aattatatgc acagccaacc ttggtaaatc
    10681 tgaatcccgg tttgaagggt gaattgaaag ctattattac cggtgataat attgttgtgc
    10741 aaacccttac tggtttaact ctcgataatc cagttcccgt tgatcttact ttagatgcct
    10801 tgggtaccat caattataaa atgaaagatg ccagtattta tgtttcagga acagagggaa
    10861 ttgtaaacaa tcagccccta aacttcgaac tagccgctat gctaaaagac aacattgtaa
    10921 ccttacaagg cttgaaagtt aatgatctaa tcaatgttag tggagcaatt aatctggcaa
    10981 actatgcaga tatgaatatt gggttaaaca tcaacaacct aaattatttg gatgtcgttc
    11041 gctactatcc caaattagat gtcaatcttc cagaatttaa tggcttgaat gtttttttag
    11101 aatataatcg ggataattta caacgtttac gagctaatgt tttcttatcg gatatagacc
    11161 ttttagcagt tactcctttt tctctggaat tgggaatgga aggtcccttg aataacattc
    11221 ttgtatttag cacaattaaa aataaagaac aaaatattgt ggatatgaca ggttatgtta
    11281 gtcttactcc tgagctgaat gttaatttgg atgcagcttt taaagacctc catattcaag
    11341 atgttcttat caattctccc atctatggaa ttatagatgg aatctcagga attacttggc
    11401 ataaggtaaa agaacaaaaa ccggaattag agctgagagc agatttaatt tcgcgagata
    11461 ttgttcttgg taactataca atagataaag caattattaa gggtaagcaa actcctcgta
    11521 aaatgatcgt agatagttta tttgttaaat ctacaggatt atttgaactt tccggttccg
    11581 gagcaattaa ctataatgct ctctataacg agtttttcga gggtcctgaa catttgaatt
    11641 tacgggctca gggtgaactt tttacttggt tgaatggtct tactccctac atccaagaag
    11701 caaaaggttc atcatcttta agcgcaagta tcggaactac agaagaacaa tttatggttt
    11761 ccactggaaa aataaatatc agcggaggct atatccagat taaagaccaa acagaaccct
    11821 gctccaatat caatatccgt ggtggattcg acaaaaatag agtggttatt gattatggac
    11881 aaatccaaat gggtgaaggc aaactagtct tcaataatat cttcgaagtc gataatagcg
    11941 atcatattat gctgggattc cttgatttag gtattttaag attgatggta gaagaacctg
    12001 gaattatgat taatgttcca aaatatacaa ctccccgttc tctaaccaaa gtaattttaa
    12061 aaggtaataa cagccgttat gcaactatta aaggaccctt cgaccaaatg aaaatcagcg
    12121 gagaggttat agcttccaat actaatgttt tgtaccctcc agatacggaa aacttattaa
    12181 ctttagcaac ctctatgcga ggtacaaata aaaaaagcag ttcagaacca tctcccttac
    12241 cctttacctt agatgtaaag cttattgcag gagaaaatgt taagtatgta acctatcccg
    12301 caaaattaag agttgttcca ggcggctatt tacatttaat gtatgatggg ctatctttca
    12361 ttgcagaaga ggcatcattc tcttctgaac aaggaaccgt agatatcttt ggcaccatct
    12421 ttcaggtaga taaagtaaat ctaaccttag ttgattccca aaatttattg ggtttggaag
    12481 ggatattttt taaacgcgct cccgatggtt ctatgataac tcttacagca gtaacttcca
    12541 acgatttaac caagcccttt atggaacgct tgcaattaaa cttagccagc gataatcccg
    12601 aagataaaac aatcagccaa attttagcgc gcttaagata tagcggctct gaaacaccag
    12661 atcaaaaaca gggagatatt ttgcaggacg aagcattaac tttaatttct ggaaacttgg
    12721 atgcctcgct ttttactcct attctttctc cagtggaaaa ctatattcgt agaacattac
    12781 atttagatag ttttaccatt aatgctggtt ttctacaaaa tctttacaca gaatatacca
    12841 gtaatccaaa acaatttgca gaatatacag atatgaatga acttagctcc gatattgcca
    12901 aatttagttc atctattttg ctgaacaacc tttccatttc aatgagtaaa tatttgggaa
    12961 gcagtttgtt tttggattat atgcttactt tacaggaagc tacaaacctc caaaaacgca
    13021 ctcgcattct ggtttccaat gaagtttcca tacgccttat tttacctgat aattaccgtg
    13081 tgtcttacac cctgaaaaat gaagcacagg agaaaaagac cagtcacgaa attatgctgt
    13141 ctcgatctta tcgcttttgg ggactttaga aacaaggatt ttaggattag atttaagaaa
    13201 cgaggattta aggatttta
//
LOCUS       DAORHB010000018        13089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_3829_length_13089_cov_5.315099, whole genome shotgun sequence.
ACCESSION   DAORHB010000018 DAORHB010000000
VERSION     DAORHB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13089
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3829_length_13089_cov_5.315099"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PKM80_01130"
     CDS             complement(<1..128)
                     /locus_tag="PKM80_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV92540.1"
                     /translation="MKHIIFNEKGFTLFETIAVITISSILIVTASMGIGVFFRKYQ"
     gene            complement(137..613)
                     /locus_tag="PKM80_01135"
     CDS             complement(137..613)
                     /locus_tag="PKM80_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92541.1"
                     /translation="MKKNIHTLPENNSPNERSFNFSVLGSQGGYSLAEVLVVAIVLAV
                     MVLTVYIGIEFAEKKITQDYRQRVATLLLTGELEKQYTLYMKENIFRPFTEVPVEIDK
                     TEELTVTGMMSISVGREVENYMTHTYGYQYVIGEIRWVDPVTEKVHFVRLREDFYD"
     gene            complement(616..1671)
                     /locus_tag="PKM80_01140"
     CDS             complement(616..1671)
                     /locus_tag="PKM80_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNV92542.1"
                     /translation="MTIHELLRFTAEAGASDLHIAAGAHPMVRVNGRMKKLNLPILSP
                     EEVETLVFGVMNESQQALFKKNLEIDFSTKLSNDVRFRVNAFHQINGVSCAFRVIPNE
                     IKSYEELHLPDILRHLTTKEKGLILVTGPTGSGKSTTLATMIDSINEGRNCHIITVED
                     PIEFVHRSKNSLINQRELGHDTWSFTAALRSALREDPDVILVGEMRDLETVSLALTAA
                     ETGHLVFATLHTGSCTKSIDRIIDMFPKEQQQQIRSMLSESLEAVIAQTLLPTKDGKG
                     RVPSVEIMIANAAVRNLIREEKTYQIPSVIQASTKEGMQTRDQSLYNLVMNNLIERTI
                     AEQFADNPKQFATGAGF"
     gene            complement(1691..2128)
                     /locus_tag="PKM80_01145"
     CDS             complement(1691..2128)
                     /locus_tag="PKM80_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV92543.1"
                     /translation="MLRKLKNQKGFTLIEILVVVIIVAILAALAVPRYLRYVEKSRST
                     EAQTAINTIRKTYDIYMQTNGSTDGFSVEQALKEANLGESTVKNWKFEVVGNPPKKYI
                     ATSTQDFAGGEGKQVWYDVTDAQFHGYGIDTWTNPETGGMETD"
     gene            2466..4505
                     /locus_tag="PKM80_01150"
     CDS             2466..4505
                     /locus_tag="PKM80_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV92544.1"
                     /translation="MSRSLSFYLGLLLLCINITFLGADNTPKPVSNDKRINETDILAE
                     FDGGVITRKDVEAKISKLPVQYQARYKTVEGQTEVLNAMAMEEVFYKKAQQMGLENDP
                     IVLERIANIEKRFYIQEYYKRNVSDLVVITEDDLQSYYNQNMSQFYLNPYIEIDYLQA
                     ADENEAKKALEELKKGVSFAEVSDKYNQNTYAKGLKGRIKNIRLNGNIPGIGNDYELE
                     NLIRTSKVDSLSFIGPVNTSYGWHIFRTVNWVDGRQKDFSEVKQEIEQRLRPLKEREL
                     LDSIIENSKIKYAVVIDSSLVNTITLDANKRNNNNPILDNVVLSSSDPDLRFTVRQLL
                     QIYDKLSPQEQQFVIKGGGINLLINQELTQYLMYLEAKAAGYDKYIADNEDYLQAKRA
                     YILRRAYEKLITENIEISDEEIAQRYERDREQFSSPEYRTIEVLFFKDKKTAEKALKK
                     YSKAYKKNNEKAMQDVIKKYSIKPNNAIMDYQYRNGIITGLGDDAKFSNMIWDNPVGY
                     LSPVFTTAKGDIVFFRTIKETPKVYKSKLEVEPRIYGMLKAEKEKAKQAEVTENLYVE
                     FNMKKYPERITLTMTAEELFTQADNAARQRNYRDAIVYYDQIINHYKNNVDDYKASFM
                     KAFLVAEEMKQTDLALQLFREFLQKYPTGDLNESAQFMIDSLEGKIPEHFEPVED"
     gene            complement(4826..5587)
                     /locus_tag="PKM80_01155"
     CDS             complement(4826..5587)
                     /locus_tag="PKM80_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92545.1"
                     /translation="MKWRLIFLCIFCFILGEALFAQTDSLKTSQYINSGELTQLPDSL
                     DKAIRDAEQKKNADLFYNLGVDFFARGDIGMANLYFLKALNINSAHSQAKNNLNLSIR
                     YSPDADLYPEHLFLVQVLYKVLDFFSVNRLAIFVLIFLFLSALALCWLLFYDPEKERA
                     LPILVLALCLIICIASSITLGIKLHHQKHNPNAVVILNQTNLLSPTSNNVIQTVHSGL
                     IVKIVQSENNHYLVHLPNGQLGRLKKEAVKTVIGS"
     gene            complement(5584..7365)
                     /locus_tag="PKM80_01160"
     CDS             complement(5584..7365)
                     /locus_tag="PKM80_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNV92546.1"
                     /translation="MKKTILLIMLFPLMLSALKIDLSVNKYVISTNDQLEVTLKITDS
                     DRIKVVEPTPPSIPLFSFRNMTSGSSSSVVFEGTKLVSEFSETFRFFYFPLKTGKTTI
                     PSFQVKVNGRFYSTREINIEVVKGTSKAPSQSAPSYPNFGAFGFDEPDSWDDREQWIG
                     DSFLLAIPQEQTIYRGFPTIVSYYLYTDEMVRSFNLENEIDSDGYGKSTYEQPSVLNY
                     ENVNLNGKQYKRALIKRLAIIPNKEGRLQAPILEGYARLYRFSYSNKTLRSEGGIINV
                     LPLPKNNVPASFGGAVGDFKISYSLSRKELSLGETITFTLKIKGRGNFNQFSAPEFAS
                     GKGFQVSTPMVIDNLKAGIEGERTYYYTLIPQDKGSYELPELSFTWFDNYSGSYKTYR
                     SPKEMIKVSSANVLSYFNRLRQSKTPKAMLPKVVKKHFPPYVPYVQQPWYWILVLLIF
                     AFSLFVAFFALDSKMKRDNPERYASLKAEKVLLRYLKPATLAAKNLSLEFYPLAEKAL
                     FEYLASKYKLSNRLSTKEKLQALSQENIPDWLLEEIQLFLNHSQAARFMPESERAVNL
                     DEDLNRIRKIFNGFTRLGKNKHNRSTK"
     gene            complement(7367..7951)
                     /locus_tag="PKM80_01165"
     CDS             complement(7367..7951)
                     /locus_tag="PKM80_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV92547.1"
                     /translation="MSIKAKRIILLVIVIIILMLILELLLRPKVIRQSFADSFYQAKK
                     YNKAENLFRKNTTNNDAAAYANLAKSLYKQNRFAEADSAISIALNNAQDKKDILYDRG
                     NIAYLQKDYQKALKDYKEALLLNPNDDDLRANYELTLRKLQKQPNPQPLMQQNEDKHK
                     DEDILNILKGLDNKESTDRQQQKPKQSFKTDKWW"
     gene            complement(7941..8936)
                     /locus_tag="PKM80_01170"
     CDS             complement(7941..8936)
                     /locus_tag="PKM80_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV92548.1"
                     /translation="MNIANPYYLALLAFCLIFLILLFRRENRLRRRFQKYAELHLKEH
                     YYHSQSPFWIGFKLFICILALGCIIIALTRPQWDYEKKDLQSSGMDIIFAIDVSKSMD
                     ATDMMPSRLLRAILQIGSFLDQVKTDRIGIIAFAGTATLQCPLTDDYEAVRIVLNGLN
                     SNSVEIPGTNIGKALALAAEAFPTDSKANTLILISDGEDLENSVLRKAKELKAKGIRV
                     YTMGVGSPKGTIIRHPETGEEVKTFLDESTLQEIAELTNGEYFRVTPGGEEIQLILKR
                     IYESESIRRGKKVTILKEQYYLFGILALLLLIIESLLDPRKRKRGLMAAEHSDEH"
     gene            complement(8933..9934)
                     /locus_tag="PKM80_01175"
     CDS             complement(8933..9934)
                     /locus_tag="PKM80_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV92549.1"
                     /translation="MIKFGHPWLLLLLLIIPLYLVWELKFNNRRRNYLPHSRVKQLRF
                     LSHNQYLYRYLFPVLRALIIFFLCIAIAEPRWGTRSRDLSNKGVDIIMAIDISGSMLA
                     MDFAPQNRLSAAVSVAKDFVKKRPNDRFGLVAFSEYALTQVPLTFDHLAMLNSLEKLK
                     VNEEASATALGMGLAKAVARLKNSTAKSRVIILITDGVNNTGEIDPLTAAEMAKELGI
                     KVYPIGVGSKGLVPFPYSDPVFGTRYINTLIDLDMETLNKIATTTGTGKATLATDAKS
                     LADIMNKIDRLEKTQFTTQFRYNYSEQFMPFLWLAFIFLAIELLLKTVILPILPDQL"
     gene            complement(9931..10812)
                     /locus_tag="PKM80_01180"
     CDS             complement(9931..10812)
                     /locus_tag="PKM80_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92550.1"
                     /translation="MKKSSVLAFSLIFFPLIIWAFHQELQGADSLKVGTPFLLKITAD
                     YPIHSVSIPDTLEDFAILESRQDERNTRSWQIKIAPLKIGALSFPRIAVNPVDSSVTP
                     VYTDGFRVYVLSVLAEGDTLLRDIKPLQKYPLQLGIWVYLILLLIAIALAIYLWLKRP
                     KKPKREYITQPETKQAILPAWKKSIQALDELIKEDLLAKGEVVLYHFRLSNILRGFLE
                     ETYHFPALEMTGNEIITYLNKRNIPNYREIRAFINYCDMVKFAKRQPSLQEIENYTLW
                     LKDYLISFIGNTIEAKN"
     gene            complement(10809..11693)
                     /locus_tag="PKM80_01185"
     CDS             complement(10809..11693)
                     /locus_tag="PKM80_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV92551.1"
                     /translation="MFTQSVSDILKKIRRIEIRTRNPVAEMFRGEYHSRFKGQGLEFS
                     EVREYQPGDNYRDIDWNVSARLGQLYIKKYRETRELRVVFLVDVSSSQEFGTRSMIKR
                     ERIAELVAALSFSAVANQDLAGLILFSDQVERFLPPRKGRNNALEILREVLYYEPKRK
                     KTSLQAACEYAYKMLKKRCIIFILSDWIDSGYERSLKILAAKNEVVALQVVDDSELEL
                     PPAGILSLKDPETGEEIWINSSDPKLRKAYQEVINNEQKELQEFFRECNCDYLMIKTS
                     DSYVSSLRKFFALRGKRR"
     gene            complement(11710..12696)
                     /locus_tag="PKM80_01190"
     CDS             complement(11710..12696)
                     /locus_tag="PKM80_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV92552.1"
                     /translation="MLIEAIQNKVEQSSVALENVRLEIARVIVGQQEIIDRLLIGILA
                     NGHILIEGVPGLAKTLIISSLASVFDASYSRIQFTPDLLPADITGTMIYNPKSGDFSV
                     KKGPVFANFILADEINRAPSKVQSALLEAMQERQVTLGDNTFILPKPFFVMATQNPIE
                     QEGTYPLPEAQVDRFFMKLKIKYPSYDEERIILERMVNEQPIPLKKVLSPADIESMRE
                     VMYMVYMEEKLKDYILHLINSTRHPERYPRLNDLKGLIEFGASPRATIYLARAAKANA
                     YISGRAYVIPDDIKAIARDVLRHRIILSYEAEAEEITTEDIINRILDEIEVP"
     gene            complement(12826..13017)
                     /gene="rpmB"
                     /locus_tag="PKM80_01195"
     CDS             complement(12826..13017)
                     /gene="rpmB"
                     /locus_tag="PKM80_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNV92553.1"
                     /translation="MSKVCDICGKTAQVGNYRSHALNATKRRFYPNLHKVRVEDEKGT
                     KRISVCSSCLKANKVRKVI"
BASE COUNT         4170 a         2457 c         2316 g         4146 t
ORIGIN      
        1 tcttgatatt tacgaaaaaa gacacctatt cccatagagg ctgttactat taatatggat
       61 gagatcgtaa taacagctat ggtttcaaat aatgtaaatc ccttttcatt gaatatgata
      121 tgtttcatgg ctcgtcctaa tcgtaaaaat cttcgcgcaa acgcacaaaa tgtacttttt
      181 ctgtaacggg atcaacccaa cggatttctc caattacata ttgataacca tatgtatgag
      241 tcatatagtt ctcaacttcc cttcccacgg atatagacat cattccagtg acggtcaatt
      301 cttctgtttt gtcaatttct acaggaactt cagtaaacgg tcggaaaata ttttccttca
      361 tatatagggt atattgtttt tccagctcac cagttagtag caaagtagca acgcgctgtc
      421 tataatcctg agtgatcttt ttttcggcaa actctatacc tatatataca gtaaggacca
      481 tcaccgccaa aacaatggcg actacgagaa cttcagccaa actataccct ccctgagaac
      541 caagaacgct aaaattaaag cttctttcat ttggcgaatt gttctcaggt agggtatgaa
      601 tatttttctt cattcctaaa aacctgctcc tgtagcaaat tgtttaggat tatctgcaaa
      661 ttgttccgct attgttcttt ctatcagatt attcatcacc aagttataca aagattgatc
      721 tctggtttgc ataccttctt ttgtacttgc ttgaataaca gaaggtatct gataggtctt
      781 ttcttcacga atgagatttc gaacagctgc attagctatc ataatctcaa cggaaggaac
      841 acgacccttt ccatcctttg tcggcaaaag ggtttgagca ataactgctt ccagtgattc
      901 ggaaagcatt gaacgaatct gttgttgttg ctctttaggg aacatatcaa tgatacggtc
      961 aatcgatttt gtacaacttc cagtatgcaa agttgcaaac accaagtgac cagtttctgc
     1021 agctgtcaac gctaaggaca ctgtttccag atcgcgcatt tcaccaacca gaataacatc
     1081 aggatcttca cgcagggcac tacgcaaggc agcagtaaag ctccaagtat catgaccaag
     1141 ctcacgttgg ttgattaagc tatttttact gcgatgtaca aattcgatag ggtcttcaac
     1201 tgtaataata tgacagttac gaccttcgtt tatgctatca atcattgtag ctaaagtagt
     1261 agattttccg ctacctgtag gtcctgtaac caggatcagt cctttttctt tggtagttaa
     1321 atgtcttaaa atatctggaa ggtgtaattc ctcataactt ttaatttcat tggggattac
     1381 acgaaaggca caagatacac catttatctg gtgaaatgca tttactctga atcggacatc
     1441 gttactcagc ttagttgaga agtcaatctc caaattcttt ttaaatagtg cctgttgaga
     1501 ttcattcata actccgaaaa caagggtttc tacttcttcc ggacttagaa taggtaaatt
     1561 tagttttttc atacgaccat ttacgcgcac cataggatga gcgccagcag ctatatgaag
     1621 gtcagaagct ccagcttctg cagtaaagcg taataattca tggattgtca agttactatc
     1681 ctcctcgggg ttaatcggtt tccattcctc cagtttcagg attggtccaa gtatctattc
     1741 cgtaaccatg gaattgggca tctgtaacat cataccaaac ttgttttcct tcgccaccgg
     1801 caaaatcttg agtagaagtt gcaatgtatt ttttaggtgg gttaccgaca acttcaaatt
     1861 tccaattttt aacggtgctt tcaccgaggt tggcttcttt aagggcttgt tcaacgctaa
     1921 agccatctgt tgaaccatta gtttgcatat agatatcata agtcttacga atggtgttta
     1981 ttgcagtttg ggcttctgta ctacgagatt tttctacata acgaagataa cggggaacgg
     2041 ctaaggccgc cagaatggct acaataataa ccaccactaa gatttcgatt agggtaaaac
     2101 ccttttggtt tttcagtttc ctgagcattt ttttatcctc ctacaggaat gtttttttta
     2161 cttttatata tatgcacaat gcgtgccaaa tcccaattaa aaatttgaat aattgaaaat
     2221 agggagagtg taaacagcta tagcacaaga aaaagcgttg tttggttata tagttgggcg
     2281 aatgttgaaa accgagataa gcaaattatg ggcaacctga aaaagtaaaa aagaaccgca
     2341 gatatttaaa ctataaatgc tttttcaaaa tcgtatatac cttatagtat aaaacaacac
     2401 ttgacttctt ttactatctt acaactttgt ctcacagaca aaaataattt attaaggagc
     2461 atacgatgtc ccgatcttta agtttctacc ttgggttgct cttactgtgt atcaatatta
     2521 cttttttagg agcagataat acccctaaac ctgttagcaa tgataagcga ataaatgaaa
     2581 cggatatttt agcggaattt gatggcggag taataacccg taaagatgtt gaagctaaga
     2641 tttccaaact tcctgttcaa taccaagcgc gttataaaac agttgaaggt cagacggaag
     2701 ttttaaatgc tatggctatg gaagaagttt tttataaaaa agctcaacaa atgggactgg
     2761 aaaatgatcc tattgttttg gagcgaatag ccaatattga aaagcgtttt tacatccagg
     2821 aatattataa aaggaatgta agtgatttag tagttattac agaagacgat ctacaatcct
     2881 attataatca aaatatgtcc caattttatc ttaatcccta tattgaaata gattatcttc
     2941 aagctgctga tgagaatgaa gcaaaaaaag cattggaaga attaaaaaaa ggtgtttcat
     3001 ttgctgaggt ctccgataaa tataaccaaa atacttatgc caagggtttg aaaggtagaa
     3061 taaaaaacat tcgtttaaat ggaaatattc caggtattgg caacgattac gagttagaaa
     3121 atcttattcg tacttctaaa gtagattctc tatcttttat tggaccagtg aacacttctt
     3181 acggatggca tattttccgt actgttaatt gggtagacgg gcgtcaaaaa gatttttctg
     3241 aagtaaagca agaaatcgaa cagcggcttc gtcctctgaa agaaagagaa ttattggatt
     3301 ctatcataga gaattctaag atcaagtatg cagttgttat cgatagctct ttagtaaaca
     3361 caatcactct tgatgctaat aaacggaata ataataaccc tattctggat aatgtagttc
     3421 ttagttccag tgatccagat ttacgattca ccgtgaggca gttattacaa atttacgata
     3481 agctatcacc tcaggaacaa caattcgtta tcaagggtgg tggaattaac ctgttaataa
     3541 atcaagagct cactcaatat ttgatgtatc tggaagctaa ggcagctggc tatgataaat
     3601 atattgctga taatgaagat tacttgcaag caaaacgtgc ctatatctta cgcagagctt
     3661 atgaaaaact tattaccgag aatattgaaa tcagcgatga agaaattgct caacgctatg
     3721 aacgggatag agaacagttt tcctctcccg aatatcgcac tattgaagtc ctcttcttta
     3781 aagataaaaa aacagctgaa aaagcactga aaaaatacag caaagcctat aagaaaaata
     3841 acgaaaaagc aatgcaagat gtaattaaga aatattcaat caaaccgaat aacgctatta
     3901 tggattatca atatagaaat ggaatcataa ctggtctggg cgatgatgca aaattttcta
     3961 atatgatttg ggataatccc gttggatatt tgagccccgt ttttactact gcaaaagggg
     4021 atattgtttt cttcaggaca attaaagaaa ctcccaaagt ttataagagt aaattggaag
     4081 tagaacctcg catctatgga atgctgaaag ccgaaaaaga aaaagcaaag caagcagaag
     4141 ttactgaaaa cctctatgtg gaatttaata tgaaaaaata tcctgaacgt atcactctta
     4201 ctatgacagc cgaagaactt tttacccaag cagataatgc tgctagacaa cgaaactata
     4261 gagacgctat tgtttattac gaccaaatta ttaatcacta taaaaataat gttgatgact
     4321 ataaagcatc ttttatgaaa gcattcctgg ttgctgaaga aatgaaacaa acagatctgg
     4381 ctttacaact tttccgtgaa ttcttgcaaa aatatcctac aggcgatcta aatgaatctg
     4441 ctcaatttat gattgattcc ttggaaggaa aaattccgga acactttgaa cctgtggaag
     4501 attaataata aatgtacata aaacgctggc tttaaaattc taagctggcg agaataaaat
     4561 aagcagctat atatataagg aagaatggaa taccgtcatt cttccttttt ctattcaaca
     4621 atcctaataa tgctgcccac agccctaaac ataaatatag tcgataaatc gctattcctt
     4681 ctttattact tgcgctgtag ttgtagtgct gatgttggga attttgccca gtatcagcac
     4741 ggcagcccta ccgtagatac taaggaagag ataagctgga attataagta aaagaatgat
     4801 tgcagaaata actaacagat ggtaatcagg atccaataac agtttttaca gcttcttttt
     4861 ttaagcgccc tagttggcca ttaggtaaat gaaccagata atggttattt tcgctttgca
     4921 ctattttaac gatcaatccc gaatggactg tttggataac attattactt gtgggggaga
     4981 gaaggttagt ttgatttaaa atcacaacag cattaggatt atgtttttgg tggtgcagtt
     5041 ttattcccag agttattgaa gaagctatgc agataattaa acacagagct aaaactaaga
     5101 ttggcagagc tctttccttt tctggatcat aaaaaagcag ccaacatagg gctaaggcag
     5161 atagaaacag aaagataaga acaaagatag caagcctgtt aacggaaaaa aaatccagaa
     5221 ctttataaag aacctgcacc aaaaaaaggt gttcaggata taaatctgca tctggactat
     5281 agcggatgga taggtttaag ttgttttttg cctgagaatg ggcagaattg atgttcaatg
     5341 cttttaagaa gtataaatta gccattccga tatctcccct ggcaaagaaa tctactccca
     5401 gattataaaa gagatcagca ttcttttttt gttctgcgtc tcggattgct ttatccaggg
     5461 aatccggtaa ttgagtaagc tcgccggaat ttatatattg tgatgttttt aatgaatctg
     5521 tttgagcaaa gagagcttct cctaaaataa agcagaagat acataagaaa attaaacgcc
     5581 acttcatttt gtgctcctgt tatgtttatt tttacccaaa cgagtaaagc cgttaaatat
     5641 cttacggatg cggtttagat cttcgtccaa atttacggct ctttccgatt ctggcataaa
     5701 tctggctgct tggctatggt ttaaaaatag ctgtatttct tccagaagcc aatctgggat
     5761 attttcttgg ctaagcgcct gcaatttttc ttttgtggat agacggttgg agagcttata
     5821 tttggaagca agatattcaa acagagcttt ttctgccaaa ggatagaatt ccagcgacaa
     5881 atttttggca gctaaagtgg ctggtttcaa ataacgaagc aaaacttttt cagctttcaa
     5941 agaagcatag cgttctggat tatctctttt cattttacta tccagagcaa aaaatgcaac
     6001 aaataatgaa aaagcaaaga taagtaacac caaaatccaa taccagggct gttggacata
     6061 aggaacataa ggtggaaaat gctttttcac tactttgggc agcatcgctt tgggtgtttt
     6121 agattgtctt aaacggttaa agtaacttaa tacatttgcc gaagaaacct ttatcatttc
     6181 ttttgggctg cggtaggttt tgtagctacc gctatagtta tcaaaccaag taaaactaag
     6241 ttctggaagt tcataacttc ctttatcctg aggaattagg gtataataat aggtacgctc
     6301 tccttcaatt cctgccttga gattgtctat aaccattgga gtagatacct gaaatccttt
     6361 cccagaagca aattcaggag cagagaattg attgaaatta cctctgccct taatttttag
     6421 ggtgaaagtg atagtttccc ccaaagaaag ctctttacgg gaaagagaat agctaatttt
     6481 aaaatctcct actgctcccc caaaagaagc tggaacattg tttttgggca agggcagaac
     6541 atttataata cccccttcac tgcgtagggt tttattggaa taactgaaac ggtataaacg
     6601 agcataacct tctaaaatag gagcttgcaa ccttccttct ttattgggaa taattgccaa
     6661 tcttttaatt agagcccgct tatactgctt tccgttcaaa tttacattct cataattcaa
     6721 aacagagggt tgctcataag tgctttttcc atagccatcg gaatcaatct cattttccaa
     6781 attgaatgag cgaaccattt catccgtata gagatagtaa gaaacaatag tgggaaaacc
     6841 acgataaatt gtttgttctt gtggaattgc taaaagaaaa gaatctccta tccactgttc
     6901 tctatcatcc caagaatctg gttcatcaaa accgaaagct ccaaaattgg gatatgaagg
     6961 tgcactttga cttggtgctt ttgatgttcc cttaactact tcaatattga tttctcgagt
     7021 agaataaaag cgtccattca ctttcacttg aaaagaagga atagtggttt ttccagtttt
     7081 taagggaaaa taaaaaaaac gaaaggtttc tgaaaactcg gaaaccagct ttgttccttc
     7141 aaaaactaca gaactactgg agccacttgt catattgcga aaactaaata gaggaataga
     7201 aggaggagtt ggttcaacaa ctttaattct atcgctgtct gtaatcttta aggttacttc
     7261 cagttgatca tttgtactga tcacatattt atttactgat aaatctatct tcaaagcaga
     7321 tagcataagc gggaaaagca taatcaacaa tatagttttt ttcatttcac caccatttat
     7381 cggttttgaa actttgtttg ggcttttgct gctgacggtc tgtgctttct ttattatcca
     7441 atccttttag aatattaagg atatcttcat ctttatgctt atcttcattc tgttgcatca
     7501 atggctgagg atttggctgt ttttgtaatt tgcgtaaagt caattcataa tttgcccgta
     7561 gatcatcatc attaggattc aaaagtaaag cttccttata gtcctttagt gctttttgat
     7621 aatccttttg tagataggca atattgcctc tatcgtagag aatatctttt ttatcctggg
     7681 cattgttcaa agctatggat atggcagaat cggcttcggc aaaacggttt tgcttatata
     7741 aactcttggc taaattggca taagctgcag cgtcattatt tgtagtgttt ttacggaaca
     7801 ggttttctgc tttgttatat tttttggctt ggtaaaaaga atcagcaaag ctctgccgaa
     7861 tcacttttgg ccttaatagt aattccagaa taagcatcaa aattataatt actatcacta
     7921 ataaaattat ccgctttgct ttaatgctca tcactatgct ccgcagccat caaacccctt
     7981 tttctttttc tgggatcaag gagggattct ataataagta aaagcaaagc caaaatgcca
     8041 aaaaggtaat attgctcttt caatatagta actttctttc ctcggcggat gctttcgctc
     8101 tcatatattc tttttaatat cagctggatt tcttctcctc caggagttac ccgaaaatac
     8161 tctccattcg taagttcagc aatctcctga agggttgact catccagaaa agtttttacc
     8221 tcttctcccg tttcgggatg acggattatt gttcctttcg gacttcccac tcccatagtg
     8281 taaactctaa ttcctttggc cttaagttct ttggctttac ggagcacaga attttcaaga
     8341 tcttctccat ccgaaattaa gattaaagta ttagctttag aatctgtcgg aaatgcctct
     8401 gcagccaaag caagagcttt tccgatattg gttcctggaa tttctacact attactgttc
     8461 aaaccgttta aaacaattct tactgcttcg taatcatcag ttaagggaca ttgtaaagta
     8521 gctgttcccg caaaagcaat aatgccaatt cggtctgttt ttacctgatc taaaaaacta
     8581 ccaatctgta aaatagcacg caacaatcta ctgggcatca tatctgtggc atccatactt
     8641 ttagaaacat ctatagcaaa tataatgtcc attccgctac tttgcaaatc ctttttttca
     8701 taatcccatt gtggtctggt gagagctatg attatacatc ccaaagcaag aatgcagata
     8761 aataatttaa aacctatcca gaaaggtgat tgagaatgat aataatgctc ttttaaatgc
     8821 agctctgcgt atttttgaaa gcgacgccgt aatctgtttt cccttctgaa aagtaaaata
     8881 agaaagataa ggcaaaatgc caaaagtgct aaataatatg gatttgcaat attcatagct
     8941 ggtctggtaa aataggtaag ataaccgttt tcagtaacag ttcaatagcc agaaagatga
     9001 aagcaagcca taaaaaaggc ataaactgct cactgtagtt atatctgaat tgggtggtga
     9061 attgggtttt ttccaagcgg tctattttat tcataatatc cgctagactt ttagcatcag
     9121 ttgctaaagt tgcttttcca gttcctgttg ttgttgctat tttattcagg gtctccatat
     9181 ccaaatctat tagtgtatta atatagcgag ttccaaaaac aggatcagaa taggggaaag
     9241 gaactaatcc tttacttcca actcctatgg gataaacttt tatgccaagt tccttagcca
     9301 tttccgctgc agttaaaggg tctatttctc cagtgttatt aacaccatct gtaataagaa
     9361 tgatcactct acttttggct gtgctatttt ttaaacgggc aactgctttt gccaaaccca
     9421 ttcctaaggc agttgcagaa gcttcttcat taacttttag cttttctaaa gaatttaaca
     9481 tagcaagatg atcaaaagta agaggaacct gagttaatgc atattcggaa aaagctacta
     9541 agccaaatct gtcgttggga cgctttttta caaagtcctt agctacacta actgcagcac
     9601 ttaatctatt ttgaggggca aaatccatag ctaacataga accactgata tcaattgcca
     9661 ttataatatc cacaccctta ttacttaaat ctcgggaacg agttccccaa cgcggttcag
     9721 ctatagcgat acaaagaaaa aatattatta aggcacgtaa aaccgggaaa aggtaacgat
     9781 aaagatattg gttgtgagat aaaaatcgca actgctttac tctggaatga ggtagataat
     9841 tccttcttct attgttgaac tttaactccc atactaaata taaaggaata atcaacaaaa
     9901 gcaacaacaa ccagggatgg ccaaatttaa tcaattcttt gcctctattg tgttaccaat
     9961 aaaactaatc aaatagtcct tcagccataa agtgtaattc tcaatctctt gtagcgaggg
    10021 ttggcgtttg gcaaatttaa ccatatcaca atagtttata aatgctctaa tttcccgata
    10081 attcggaata ttgcgtttgt ttagataagt gatgatttcg ttacctgtca tttccaaggc
    10141 gggaaaatga taggtctctt ctaaaaagcc acgcaaaata ttggataacc gaaaatgata
    10201 taaaaccacc tctcctttgg caagtaaatc ttctttaata agctcatcta acgcttgtat
    10261 agattttttc caagcaggta aaatagcttg tttagtttct ggctgcgtta tatactctcg
    10321 cttaggtttt ttaggacgct tcaaccaaag ataaattgct aaagctattg cgattaataa
    10381 taaaatcaga tacacccata ttcctaattg taaaggatac ttttgcaaag gtttaatatc
    10441 ccttaaaaga gtatcccctt cggctaaaac tgaaagaaca tacactctaa aaccatcagt
    10501 gtaaacagga gttacagagc tgtcaactgg atttactgct atgcgtggaa aagataaggc
    10561 ccctattttt agaggtgcta ttttaatttg ccagctgcgg gtattccttt catcctgtct
    10621 gctttccaat atagcaaaat cctctaaagt atcagggata gaaacactat gtattgggta
    10681 atcggcagtt atttttaaga ggaaaggagt tccaactttt aagctgtctg ctccttgaag
    10741 ttcttgatgg aatgcccaaa ttatcaaagg gaaaaagata agggagaaag ccaaaacgct
    10801 ggattttttc atcgtctttt tcctcttaga gcaaagaatt ttcgtaatga acttacataa
    10861 gaatctgaag ttttgatcat tagataatca caattacatt cgcggaaaaa ttcctgcaac
    10921 tctttctgtt cattatttat tacttcttga taggccttac gcaatttggg atcggaacta
    10981 tttatccaaa tttcttctcc ggtttcggga tccttcaagg ataaaatgcc tgcaggggga
    11041 agttctagtt cgctatcatc tacaacctgt aaagcaacaa cttcattttt agcagccagg
    11101 atttttaagg agcgttcata tccagaatcg atccaatctg ataaaataaa aataatacag
    11161 cgttttttca acattttata ggcatattca caagctgctt gcagggatgt ctttttcctc
    11221 tttggctcgt aatagagaac ttcgcgaaga atttccaaag cattatttct tccttttcga
    11281 ggaggcaaaa aacgctctac ttggtcagaa aaaaggatca ggccagctaa atcctgattg
    11341 gccactgcag aaaaagataa agctgccact aattcagcaa tgcgttctct ttttatcata
    11401 ctacgggtgc caaattcttg tgaactactt acatcaacca agaaaactac tctcaattct
    11461 cgggtctcac ggtatttctt tatgtaaagc tgacccaaac gcgctgaaac attccagtca
    11521 atatctctgt aattatctcc gggctgatat tcccgcactt ccgaaaattc caaaccctgt
    11581 cctttaaacc gactgtgata ttctccacga aacatttcgg caacgggatt tcgcgtccga
    11641 atttctatcc tgcgaatttt ttttaggata tcggaaacac tttgggtaaa cataagcaga
    11701 gatatatctt taagggactt ctatttcatc caaaattcga ttaattatat cttcggtagt
    11761 aatttcttca gcttctgctt cataagaaag aattatccga tgccttaaaa catctctggc
    11821 aattgctttg atgtcatctg gaataacata agccctacca gatatataag cattggcttt
    11881 tgctgctctt gccagataaa ttgtagcgcg gggtgaagcc ccaaattcaa ttaatccctt
    11941 taaatcgttt aagcgaggat aacgttctgg atgacgagta gaattaatca agtgtaaaat
    12001 ataatccttc agcttttctt ccatatatac catatacatc acttcgcgca tactttctat
    12061 atcagctgga cttaatactt ttttcagagg aattggttgt tcatttacca tcctttccaa
    12121 aattattcgt tcttcatcgt aagaaggata tttgatcttc agtttcataa aaaatctatc
    12181 tacttgagct tctggaagag gataagttcc ttcctgttca attggattct gagtggccat
    12241 cacaaaaaac ggtttaggca gtataaaagt gttatctcct aaagtaactt gcctttcctg
    12301 cattgcttct aaaagtgcag attgtacttt ggaaggagca cgattgattt catcagccaa
    12361 gataaaattg gcaaagacgg gacccttctt cacactgaaa tcgccacttt tgggattgta
    12421 aatcattgtg cctgttatat ctgcaggtaa caaatcaggt gtaaactgaa tcctggaata
    12481 agaggcatca aaaactgaag ctaaggaaga tattatcagg gtcttggcta agccaggcac
    12541 accctcaata agaatatgac cattggcaag aattccaatc agcagacggt ctataatttc
    12601 ttgctgacca actatcactc gggcaatttc caagcggaca ttttcaaggg ctactgaact
    12661 ctgttctact ttgttctgaa ttgcctcaat taacataaat cccccatatt ttattcactt
    12721 actttaagta aaaaatcggg aatctgacca aaccaagatt cccgaaaaat gttcttctat
    12781 tttattgagt ccaggagtaa aaaacccaaa cagaacggat tattgttaaa ttactttacg
    12841 aactttattt gctttcagac aagaactaca aacacttatt cgtttggtcc ctttttcatc
    12901 ttcaacacga actttatgca gattaggata aaatctgcgc ttagtggcat ttaatgcatg
    12961 gctgcgataa ttgcctacct gagctgtttt tccacatata tcacaaactt tagacataat
    13021 tcacttccca atttgttttc ttttaaacca caaaaattat tactccattt ttgacaagca
    13081 attatttag
//
LOCUS       DAORHB010000019        12690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_4066_length_12690_cov_4.384171, whole genome shotgun sequence.
ACCESSION   DAORHB010000019 DAORHB010000000
VERSION     DAORHB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_12690_cov_4.384171"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /gene="ricT"
                     /locus_tag="PKM80_01200"
     CDS             <1..657
                     /gene="ricT"
                     /locus_tag="PKM80_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNV92554.1"
                     /translation="TSKNFSIRRHASSEDIDKMRNLSYEEEKASRTFLSILERYPFEM
                     KLIETIYQFDGNKLTFFFTAEGRIDFRTFVRELATVFKTRIELHQTSGRDEARRLGGF
                     GMCGKQYCCGSFLKRFNQVTIKMVKDQNLAGNLTKISGPCGRLLCCLNFEEDFYVEEV
                     KGFPLIGTFVSYQNTKMMVFRLNVIGKKIMLASEEGIIQEIDLDEFSKLPIISLPILE
                     "
     gene            657..1709
                     /gene="rlmN"
                     /locus_tag="PKM80_01205"
     CDS             657..1709
                     /gene="rlmN"
                     /locus_tag="PKM80_01205"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNV92555.1"
                     /translation="MFTNIYSLMPEELANKIKIEQLGSPVYRTKQLLFWLYKSYINEP
                     DKMSNLPAEFKTYLLNNYSFYLPETDSKLVSSDKAIKYRLKLEDNELIESVLIPTEKK
                     NTLCISTQVGCGRNCQFCSTAKMGLIRNLYNYEIVGQVIYAARQLKSSDNKKLTNLVL
                     MGMGEPLDNLDNVLSALRILQSQEGLSFSPRRITVSTSGVVPGIIALADSGIKAKLAL
                     SLTSAIDSKRSKLMPVNKQYNLIELKQALLYYLRKSSYRITIEYILIPSFNMDDDDLK
                     ALSKYVGDLSCKINFIPYNPGKDSIFRAPTVKEINDFMYRAQSLPQAITLRKSRGADI
                     FGACGQLNKNNLGAKI"
     gene            1706..2482
                     /gene="deoC"
                     /locus_tag="PKM80_01210"
     CDS             1706..2482
                     /gene="deoC"
                     /locus_tag="PKM80_01210"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNV92556.1"
                     /translation="MKDIPQIAKDLFGDNHLAQCGGGHKICLNCYNCHLKSPERICDP
                     DTNLASVIDATILKAEAKRKDVEELCKTANLYKTASVCVNSYFIPLVKELLEQPVKIC
                     TVINFPLGASSPEAILNEAIAVLEAKAEEIDMVQNLSALLSGDYKNNLLSTQEVVNIC
                     NKHNALLKVILETCYLTEEQIIISALLAKKAGADFIKTSTGFGSQGATIENIALMREV
                     VGPKIGVKAAGGIRTKEQALAMIKAGANRIGASNVVAILQ"
     gene            2495..3916
                     /gene="thyX"
                     /locus_tag="PKM80_01215"
     CDS             2495..3916
                     /gene="thyX"
                     /locus_tag="PKM80_01215"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNV92557.1"
                     /translation="MKVNVAGYNIDKGLIDKLNSPVATPELISAAYARISRSNKSIED
                     LRKEAITELEKARKTNTNIIFEMGHSSVAEHAVFNIDILGISRALTELVQRSRIASYT
                     EKSQRYVTFGKDYIVPKEFANRPKLKQPYKQMMDTLFEEYEYCFNALNELYKETKPEL
                     KNRQRECLAKEDARYILPLSTKTQMGMTINSRSLENLLRRLSKSPLKEAAELKDLLFK
                     SVYAIAPSLIRYTEDDNFSGIVNLEKVGFTDFLQQELPWVKELDMENKLNIIHTPNNI
                     DDTVLAAIIYQQGELSWNETKETVAHLPKIIKRQLWNQVFTNLKPWHKVPRAFELIDF
                     SFEITMSESCWAQFKRHRYCTIIQKGGSALATKIPPSIKKINRDKIWSDLNTQLIRLG
                     SSLPPKLAYLSPYFRTNASLVTILAKMNLREIYHFVRLRSDENAQWEIRELSQLMAKQ
                     VKNAAPQAGFWLCGKSEFPKQSS"
     gene            4157..5614
                     /locus_tag="PKM80_01220"
     CDS             4157..5614
                     /locus_tag="PKM80_01220"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNV92558.1"
                     /translation="MKMWRQLVLVLIALLMTSCASAKDNMIDPNGPNPVVEVVKNVRE
                     AVVQIRVEAKVTVQNYINPFLDDPWFRQFFRFPQEQTRPIVSLGSGFIYEYNPGSREA
                     FILTNNHVVEKGRDGTITVTMADKVVYNASIVGLDPSTDVAVIKIVVKEGEKVTVAPL
                     GDSDKLQIGEWAIAIGNPFSEGLDRTVTLGVISATSRSNLNLGDNSPIYQDFIQTDAA
                     INSGNSGGPLLNIKGEVIGINSALASTNGGNVGIGFAIPINLAKRVVEDLVASGKVTR
                     AYIGILPQEITPDIMEAFGLKEVAGVLVAKVEKDSPADKAGIKTGDIIIEINGEKVPS
                     VPKFRIAIATARVGQQIPLKIVRDKKDMTIRVTLEAYPEDIAAASDSVKGGIATGISV
                     EAIDSQTAKRLGVTSDKGVVVTKVDPNSPAAKSGLKVGYVILQIEDKEVNSPKEFNTE
                     LEKAKTNMEKENRKTIRLYVLDTNKQPRFIVLKFE"
     gene            complement(5902..6225)
                     /gene="trxA"
                     /locus_tag="PKM80_01225"
     CDS             complement(5902..6225)
                     /gene="trxA"
                     /locus_tag="PKM80_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNV92559.1"
                     /translation="MSIVAVNSSTFEEEVLKSSLPVLVDFWAPWCGPCKALSPTVAKV
                     ANETAGKVKVVKVNVDEAQDIAAKYSIMSIPTLLVFVNGVVSDQLIGLVQKDKIMEKL
                     NKFMQ"
     gene            complement(6231..6503)
                     /locus_tag="PKM80_01230"
     CDS             complement(6231..6503)
                     /locus_tag="PKM80_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNV92560.1"
                     /translation="MKLRPVEDNVVVKLNTADNEKTIGGIIIPDTAKEKPQIAEVIAV
                     GNDEDLQKIVKVGDKVLFGKYAGTEIEFEGEKLLILSKSDLLAIVE"
     gene            6856..8031
                     /locus_tag="PKM80_01235"
     CDS             6856..8031
                     /locus_tag="PKM80_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92561.1"
                     /translation="MATISKKVLFIDTPFAIEEGLRAKRSRFLWDIVSRNFDADLLLL
                     KSDIYREKPISSHSGYDKLYSLSLKSENKLEPIIYHHLGKGQAGRFAHILDSKRYEII
                     FFGGLACLPLLKIANRVLPNCEMIIDVEKVYISDLKEKWESNRELNNISYLWEYIQQY
                     IWDKYLINKKPNCLYANPGDLDLLCNFYKINKSNSHFVPLPITDLRFSESTEKDKRYI
                     IFWGSPENKDNLTVAKNIVSEIYPRISKKLVEKNIGIILCGEEKMQEFCSGRISYAPY
                     DDLDNILEQALFIILPLEKPDTEGRILSCAAHHKALVCTKIAIQNFPVEENCYLQGDC
                     FTEVSDKILELLRGINKLNEYSDNFFNACWSNFQAAEIERNLLERINTWIKKDVTTQ"
     gene            8015..8665
                     /gene="udk"
                     /locus_tag="PKM80_01240"
     CDS             8015..8665
                     /gene="udk"
                     /locus_tag="PKM80_01240"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNV92562.1"
                     /translation="MLPLNNVRLILIGGGTCSGKTTIAKAIGMRLKDLKTVIVAQDNY
                     YRDLSNLPFQQRMKVNFDHPDAIDVDFLLSDLKLMLEGKSVDIPDYDFVTHCRKEGKT
                     CVAFADLIILEGIFALYYPELISISDLKIYVDTDSDLRLARRMQRDIVERGRDVENVL
                     DQYLSTVKPSHEAFIEPTKKNADVIIPGDRDFDKVLYMLNGYLLYELIANNREHKH"
     gene            complement(8893..10284)
                     /locus_tag="PKM80_01245"
     CDS             complement(8893..10284)
                     /locus_tag="PKM80_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HNV92563.1"
                     /translation="MNVLGFKFPIQERKTALYNLEENWYLPLLAEKRGLSLQNIKRSN
                     FGQYSMAVNYLTSVLEEAAAINKVAVYNIDHMAQLYFYGPDVVTLLNRALTGNFTDMK
                     VGQCKYSLLLNDKGGVQDDMILMRVSETSFILVINAGHDITDTVEIDNKKQEYIADID
                     RIMACKKGGEEVFAKDISDTLVKIDIQGPLSYKLIKEVYGADVLKNRNNPEKNMSFFT
                     FNEFELNGHHYYLSRTGYTNRWGWELYIPAAAAEEDAKKIITKALDFGGLLVGLGGRD
                     ENRISAGPFGLPLMGQEYDPYHTPLNAPLFETAVDMNKDYFVGKDALAKEIAEGNQKR
                     LYIFVSEGIVSNRGIYKEGKRLGTVTSSINSPNVSLEMRLAIGSKRKNVNEENGTAAI
                     GLGWFYAPLFEKDIEGKEIAEIEGKPIRIPVEFYREDENRNPIGSPVLGFVTLEGITP
                     ATAHKALKNIENL"
     gene            complement(10469..12046)
                     /locus_tag="PKM80_01250"
     CDS             complement(10469..12046)
                     /locus_tag="PKM80_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNV92564.1"
                     /translation="MNYIFTNGFVLNSQTQNFEEKSVLIHNDRIALIGNLQECKMQAK
                     SSYEIINLKNRLLLPAFLDAHTHFVEYAKGRILVNLNGCHTIDEIRNYLISYRDNLSW
                     KLKWILGGGWDRNCLNNPDELSLKLLDTIFPDIPVALMSKDYHSKLCNSLALKIAGIC
                     KDTANPKGGLIEHNSIGELTGVLYESANELIDPYIVYPESEVIIQAISETVDSIYPLG
                     LVGFNSMESIFSRDLMLKTQEKRKKFRFCWHFYPEDYEKVLQEGIKSYEGNEFYKLGG
                     LKLFGDGSLGSQTAAMFESYPQGEKGILRYTDDELFSLVLSAAENGLSSTIHSIGNRC
                     VKQVIDCFLRLKKTGKHNTLFNRIEHIQAIRNEDIPLLKSSGLFASLQPVHIANDIPL
                     INKYWSDIKEQTYSIKSLISTGIEYAFGSDAPIETINPFLGIYSAIERRQNNNPKNPQ
                     FRPEQSINPFEAIWGYTMGAAKSTKSEHKRGSIETGKLADLIVIEDFRQYPSTFWLTA
                     KSELTMINGDIVYVNEG"
     gene            complement(12140..>12690)
                     /locus_tag="PKM80_01255"
     CDS             complement(12140..>12690)
                     /locus_tag="PKM80_01255"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNV92565.1"
                     /translation="IRIVPGGSSARNGCYIATGVAIMPPAFINIGAYVDSGTLIDSHS
                     LVGSCAQIGKNVHLSAGAIIGGVLEPVGLRPVIIEDDVFVGGNTGIYEGIIVQSKAVI
                     ASGTVITASTPIYDSVQGRYLNYDSGNSFTIPYGAVVVPGSRRLKSNPEFQVACPIII
                     KYRDEKTDKAVELEQTLRELLE"
BASE COUNT         4139 a         2282 c         2215 g         4054 t
ORIGIN      
        1 acttccaaaa atttcagtat tcgccgtcat gctagttctg aagatatcga taaaatgaga
       61 aatttaagtt atgaagagga aaaagcatct cggacatttc tatcgatact ggagcgttat
      121 cctttcgaaa tgaaacttat tgaaacaatt tatcagtttg atggaaataa actaaccttt
      181 ttcttcactg ctgaagggag aattgatttc agaacttttg tacgggaact agctacagtt
      241 tttaaaactc gcatcgaatt acatcaaaca tcaggtagag atgaagctcg tcgtttaggt
      301 ggttttggta tgtgtggaaa acaatattgt tgcggtagtt ttttaaaacg cttcaatcag
      361 gtaaccatca aaatggtaaa agaccaaaat ttagcaggta atttaaccaa aatttctgga
      421 ccctgtggcc gtttactctg ctgccttaat ttcgaagagg atttttatgt agaagaagta
      481 aagggattcc ctttaattgg aacttttgtt tcatatcaga atacaaagat gatggttttt
      541 cgtcttaatg ttattgggaa aaaaattatg ctcgccagtg aagaaggaat catccaagaa
      601 attgatttgg acgaattttc caaattaccc attatcagct tacctatttt ggaataatgt
      661 ttaccaatat ctattcttta atgcccgaag agctggctaa taaaataaaa atagagcagc
      721 tcggctcacc tgtgtataga actaagcaac ttcttttctg gctgtataaa tcctacatca
      781 atgaacctga taagatgagt aatctgcctg cagagtttaa gacatattta ttaaataatt
      841 actccttcta tttaccggaa acagatagca aacttgtatc cagcgataaa gcaataaaat
      901 accgtttaaa attagaagac aatgaactta tagagagtgt gctgattcct acagagaaaa
      961 aaaatactct ctgcatttct acccaagttg gttgtggacg caattgccaa ttttgttcta
     1021 cagccaaaat gggactaatt cgcaatttat ataattatga gatagtaggt caggttattt
     1081 atgcagccag gcaactgaaa agttcagaca ataagaaact tacaaactta gttctaatgg
     1141 gtatgggcga accattggac aacttagaca atgtattatc cgccctgaga attttacaaa
     1201 gccaagaagg cctttctttt agtccaagac gaatcaccgt ttccacttcc ggagttgttc
     1261 caggcattat agctttagcc gatagtggta taaaagctaa acttgctctt tctttaactt
     1321 ctgccataga ttcaaaacgc agtaaattaa tgcctgtaaa taaacaatat aacctaattg
     1381 aattaaaaca agcattgctc tattatctaa ggaaaagttc ttatcgcatc actattgaat
     1441 atatcttgat tcccagtttc aatatggatg acgacgatct aaaagcacta agtaaatatg
     1501 ttggagatct ctcctgcaaa attaatttca ttccttataa tcctggaaag gattctattt
     1561 ttagagctcc tacagtaaaa gaaattaatg attttatgta tagagctcaa tcccttccac
     1621 aagcaataac tttacgcaaa agcagaggag cagatatttt tggtgcctgt ggacagctta
     1681 ataagaataa tcttggagcg aagatatgaa agacattcct caaattgcca aagatctatt
     1741 tggagataat catttagcac aatgtggagg cggacataaa atatgtctga actgttataa
     1801 ttgccattta aaaagcccgg agcgcatctg cgatcctgat acaaatttag cttctgtaat
     1861 tgatgccact attctaaagg cagaggcaaa acgaaaagat gtggaagaat tgtgcaaaac
     1921 agcaaattta tataaaaccg cttctgtttg tgtaaattca tattttattc ctttagtaaa
     1981 agaactcctg gaacaacctg ttaaaatctg caccgttatc aattttccct taggagcatc
     2041 ctcaccagaa gctattttaa acgaagctat agcagtttta gaagctaaag ctgaagaaat
     2101 tgatatggtt cagaatttaa gcgctttatt gagtggtgat tacaagaaca atttgctttc
     2161 cacccaagaa gtggttaata tatgtaataa gcataatgct ctgcttaaag taattttaga
     2221 gacctgctat ctaactgaag agcaaataat tataagtgca ctgttagcca agaaagctgg
     2281 cgcagatttt attaaaactt ctaccggttt cggctctcag ggtgctacca tagaaaacat
     2341 agctttgatg cgtgaagtag ttggacccaa aattggcgtt aaagcagcag gtggaattcg
     2401 aacaaaagaa caagctttag caatgattaa agcaggagcc aatagaattg gcgcttccaa
     2461 tgttgttgcc atattacaat aaaggttaca tcaaatgaag gttaatgtcg ccggatataa
     2521 tatagataag ggacttatag acaaattaaa tagccccgta gctacaccag aattgatttc
     2581 tgcagcttat gccagaatca gccgcagcaa taagagtatt gaagatctgc gtaaagaagc
     2641 tattacagaa ttagaaaaag cccgtaaaac taacactaat attatctttg aaatgggaca
     2701 cagttccgta gctgaacacg ctgtatttaa tattgatatc ttaggaattt ccagagcgtt
     2761 aactgaatta gtacaacgca gtagaattgc ttcttacaca gaaaaatctc agcgctatgt
     2821 aacttttggg aaggattata ttgtccccaa agaatttgcc aaccgtccca aactgaagca
     2881 accatataag cagatgatgg acaccctttt tgaggaatat gaatattgtt ttaatgcttt
     2941 aaatgaattg tataaagaaa caaaaccaga acttaaaaac agacaaagag aatgcctggc
     3001 caaagaggat gcgcgttaca ttttacctct ttctacaaaa actcagatgg gaatgactat
     3061 taattcccga agtttggaaa atttgctaag acgcttatct aaaagcccct taaaagaagc
     3121 agctgaactg aaggacttac tttttaaatc tgtctatgcc attgctccat ctttgattcg
     3181 ctatacagaa gatgacaatt tcagtggcat agtaaattta gagaaagtag gttttaccga
     3241 ttttttgcaa caggaacttc cttgggtgaa agaactggat atggaaaaca aactgaatat
     3301 aatccacact cccaataata tcgatgatac tgttttagcc gctattattt atcaacaggg
     3361 agaactaagc tggaatgaaa ctaaagaaac agttgcccat cttcccaaaa ttataaaacg
     3421 acagctctgg aatcaagtat ttaccaattt gaaaccctgg cacaaagttc cgcgagcttt
     3481 tgagcttatc gattttagtt ttgaaataac aatgagcgaa agttgctggg ctcaatttaa
     3541 gcgtcatcgt tattgcacaa ttatacaaaa gggaggaagc gctttagcta caaaaatccc
     3601 tcctagtatc aagaaaatta atcgagacaa aatatggtca gacctcaata cacagttaat
     3661 tcgcttagga agttcgctgc ctccaaaact ggcatattta tctccctatt ttagaactaa
     3721 cgcctccctt gttacaatct tggccaaaat gaatttaagg gaaatttacc attttgtccg
     3781 tttgcgtagt gatgaaaatg cccaatggga aattagagag ttatctcaat taatggcaaa
     3841 acaggttaaa aatgctgctc ctcaagcagg attttggctt tgtgggaaaa gcgaatttcc
     3901 taaacaatca tcctaaatca ctttaacttt ttcgcaatta tgcttattat taagatttac
     3961 ggtagtgatt ggagtatttt ttaacgaaag gaaatatggc aatcttggta agtagcaaat
     4021 tccacttgac cgtaaaccat tttcagatta taatgtatat atataaattt tattaacggg
     4081 tttacccgca ggttttttat cttttccaag tattacttag agaaaatctt atcttaaaaa
     4141 gtaaggagat ataactatga aaatgtggag acaattagtc ctggtgctaa tagcattact
     4201 gatgacgagt tgtgctagtg ccaaagataa tatgattgat cctaatgggc ccaatcctgt
     4261 agtagaagta gtaaaaaatg tacgcgaagc agttgtccaa atcagagttg aagccaaggt
     4321 aacagttcaa aattacatta atcctttttt agacgatcct tggtttcgcc aattcttccg
     4381 attcccccaa gaacaaaccc gtcctattgt ttctttaggt agtggtttta tttacgaata
     4441 taatccaggc tcccgtgaag cttttatttt aaccaataac catgttgtag aaaaaggtcg
     4501 ggatggcaca atcactgtta cgatggctga taaggttgtc tataatgctt ctatcgtagg
     4561 tttagatccc agcactgatg tagccgtaat taaaattgta gtaaaagaag gcgaaaaggt
     4621 aacggttgct cctttaggag attccgataa attacaaatt ggtgaatggg ctattgctat
     4681 tggaaatcct ttcagtgaag gattggatag aactgtaaca ttaggtgtaa tatctgccac
     4741 cagccgttct aatcttaatt tgggagataa ttctcctata tatcaggatt ttatccaaac
     4801 tgatgcagct atcaatagtg gaaacagtgg tggcccttta ttgaacatta aaggtgaagt
     4861 tatcggtatt aattctgccc tggcaagtac taatggaggc aatgttggaa tcggttttgc
     4921 cattcctata aacctggcta agagagttgt tgaagatctt gtcgccagcg gaaaagttac
     4981 ccgtgcctat attggtatct tacctcaaga aataaccccc gatattatgg aagctttcgg
     5041 cttaaaagaa gttgccggag ttttagtagc caaagtagaa aaagactctc ctgcagataa
     5101 agccgggatt aaaaccggag atataattat cgaaataaac ggagaaaaag tccccagtgt
     5161 tcccaaattc cgcattgcta ttgccacagc cagggtggga cagcaaatac ccttaaaaat
     5221 agtgcgcgat aaaaaagata tgactatccg cgttacctta gaagcatacc ctgaagatat
     5281 tgccgctgcc tccgatagtg taaagggtgg aatcgctact ggaataagcg ttgaagctat
     5341 tgatagtcaa acagctaagc gtttaggagt tactagtgat aagggagttg ttgttacaaa
     5401 agttgatcca aattcaccag cagccaaatc cggattgaaa gttggatatg taatattaca
     5461 aattgaagat aaagaagtga atagccccaa agaattcaat actgagctgg aaaaagctaa
     5521 aactaatatg gaaaaagaaa atcggaaaac tatccgcctg tatgttttgg ataccaataa
     5581 acaaccccga ttcatcgtgt tgaaattcga ataaccttca ctttctccaa aaattgaaag
     5641 agcggaagat atattgcctt ccgctctttt attattccac acttttatcc cgaaaaaagg
     5701 tttgttcttc cataattacc taccgtgtag atgcaatggt acttcttggg aatttcccga
     5761 aaagcgcccc ggtagctcca cctcaactaa ctaagcaagg acaacctttt ccccatttta
     5821 acaaagccat attttcccaa aattaaaaaa gcccggaatg cttgttacat accgggcttt
     5881 aactatagtt tcagggcaaa actattgcat aaacttattt agcttttcca taattttgtc
     5941 tttttgtacc aaacctataa gctgatcgct aactacacca ttaacaaaga caagcagggt
     6001 cggaatggac ataatggaat attttgctgc aatatcctga gcttcatcca cattcacttt
     6061 tactacttta acctttcctg cggtttcatt agcaaccttg gcgacagtag gactaagtgc
     6121 tttacaaggt ccacaccagg gagcccaaaa atccactaat actggaagtg aggattttag
     6181 aacttcctct tcaaaggtgg aagaatttac tgctactata gacattttta ttactctact
     6241 atagccagaa gatcactttt ggaaaggatt aaaagcttct ctccctcaaa ttcaatttcg
     6301 gtaccagcat atttgccaaa gagaactttg tctcccacct tcacgatttt ttgtaaatcc
     6361 tcatcattgc caacagcaat cacttcagca atctgaggtt tttctttagc ggtatcggga
     6421 attataattc cgccaatggt cttttcatta tctgcggtgt taagcttaac taccacattg
     6481 tcttcaacag gtctgagttt cattttaacc tccattaatt atctttcttg ttcattaata
     6541 atataagtga ttatggtcta tttagcaaac acaaaattag actgctaatt ttttgtcaag
     6601 ataaaaatac ttttcctgca tccgttcttt agattatgct accataaaaa aaatagcttt
     6661 tcacacaaat gaaaatacac ttggaaagag gattttgcag cttttacaaa ttatacaaat
     6721 actcagtact tttttttagg atatctatct tctttatata acgatatctt cgcctcaatt
     6781 aagttctcaa tccatttggt gcttgacata gtttgctatt tcaaaattaa aggaatagga
     6841 gatatataag ataagatggc tacgatttca aaaaaagtac tgtttattga tactcccttt
     6901 gcaatagaag agggattaag agcaaaacgc tctcgttttt tatgggatat tgtttcccgg
     6961 aattttgatg ctgaccttct tcttttaaaa agtgatattt atagagaaaa gcccatctct
     7021 tcccatagcg gttatgacaa actatattca ttgagtttaa aatctgaaaa caaactggaa
     7081 ccaattatct atcatcatct gggtaaaggt caagcaggac gttttgctca tattctagat
     7141 agcaagagat atgagatcat ctttttcgga ggattggctt gtttaccttt actgaagata
     7201 gctaataggg ttttgcctaa ttgtgaaatg attatagatg tagagaaagt gtatatatcc
     7261 gatttgaaag aaaaatggga aagcaataga gaacttaata atatttccta tctttgggaa
     7321 tatattcaac agtatatttg ggataagtac ttgattaata aaaagccaaa ttgtctttat
     7381 gccaatcctg gagatttaga tcttctttgt aatttctata aaattaacaa gagcaacagc
     7441 cactttgttc ctttacctat aactgattta aggttttctg aaagcacaga aaaggataag
     7501 agatatatca tattttgggg ttcaccggaa aataaagata atcttacagt agctaaaaat
     7561 attgtatccg aaatttatcc tcgtatttcg aaaaagctgg tagaaaagaa tataggtatc
     7621 atcctttgtg gagaagaaaa aatgcaggaa ttttgcagtg gaaggatatc ttatgctcct
     7681 tatgatgatt tggacaacat attggaacaa gcattgttta ttattctacc tttagaaaaa
     7741 cctgatactg aaggcagaat tctttcctgt gcagcacatc ataaagcatt agtttgcaca
     7801 aagatagcga ttcagaattt tcctgttgag gaaaattgct acttgcaggg agattgtttt
     7861 acagaagtgt ctgataagat attggagctt ttacgcggga tcaataaact aaatgaatac
     7921 tctgataatt tctttaatgc ttgttggtca aattttcaag cagctgaaat tgagaggaat
     7981 ttgttagaaa gaataaatac ctggatcaaa aaagatgtta ccactcaata atgttcgttt
     8041 aattttaatt ggagggggaa cctgttctgg taaaactact attgctaaag caattggtat
     8101 gcgtttaaaa gatctaaaaa cagttatcgt ggctcaggat aactattata gagacctaag
     8161 taatctgcct tttcagcaac gaatgaaagt taattttgat catcctgacg ccatcgatgt
     8221 tgatttttta ctttccgatc taaaacttat gcttgaaggg aaatcagttg atattccaga
     8281 ttatgatttt gttacgcatt gccgaaaaga agggaaaacc tgtgttgctt ttgcagatct
     8341 tattatatta gaaggtattt ttgctcttta ctatcctgaa cttatatcta tatcggatct
     8401 gaaaatctat gtggataccg attctgattt acgcttagca agaagaatgc aaagagatat
     8461 tgtagaaaga ggaagagatg ttgaaaatgt attagaccaa tatttaagca ctgtaaaacc
     8521 ctcccatgaa gcatttattg aacccacaaa aaagaatgcg gatgttatta ttcccggaga
     8581 cagggatttt gataaggttc tttatatgct aaatggatac ctgctttatg agctgattgc
     8641 caataacaga gagcataaac attaattctc tgcaagtgaa aagataaacc ccaaacaatc
     8701 aactttcctc ttaataatac acttgcaaaa gagctatctt ggctaaaaca taattaattt
     8761 taacggaatg aatccctgct gcctaatgaa taataggcag gataaatgtt attcagccat
     8821 tgatgctttg aactttgtta tcttttacaa aagcttaatt tcttgttttc taatgtttac
     8881 atttttgtag atttacaggt tttctatgtt ctttagtgct ttatgagctg tggcaggagt
     8941 tattccttcc aaggtaacaa atcctaaaac agggcttcct atcgggttac gattttcatc
     9001 ttcacgataa aattctactg gtatacgaat tggcttacct tcaatttctg ctatttcttt
     9061 cccttcgatg tctttttcaa aaaggggagc ataaaaccaa cctaaaccta tggcagcagt
     9121 tccattttct tcatttacat ttttacgctt ggaaccaata gctaaacgca tttctaaaga
     9181 tacattagga gaatttatgc tgctggtaac agttcctaaa cgctttcctt ctttataaat
     9241 tcccctgttg gaaacaatcc cttcagaaac aaaaatataa agtcgtttct gattaccttc
     9301 tgcaatctct ttagctaagg catcttttcc tacaaagtaa tccttgttca tatctactgc
     9361 tgtttcaaaa aggggagcat ttagaggtgt atgataagga tcatattctt gtcccattag
     9421 cggtagacca aaaggtccag ctgaaattct gttttcatct cgtccaccta aaccaactaa
     9481 aagtccgcca aaatctagag ctttagtaat tatcttttta gcatcttctt cagctgctgc
     9541 agctggaata taaagttccc agccccatct attagtataa cctgttctgg ataaataata
     9601 atgatgcccg tttaattcaa attcgttgaa agtgaaaaaa ctcatgttct tttcaggatt
     9661 attacgattt ttaagcacat ctgccccata gacttcttta atcaatttat aagaaagtgg
     9721 accctggata tctattttca ctaaagtatc cgaaatgtct ttagcaaaaa cttcttcgcc
     9781 cccttttttg caagccataa tccgatctat atcagcaata tattcttgtt tcttattatc
     9841 gatttctaca gtatcagtta tatcatgtcc agcattgata accaaaatga aactggtttc
     9901 tgaaaccctc attaaaatca tatcatcttg aactccaccc ttatcgttca agagcaaaga
     9961 gtatttacac tggcctactt tcatatcggt aaaatttccg gttaaagcac gatttaacag
    10021 ggttacaaca tcaggtccat aaaaatataa ttgtgccata tggtctatgt tataaactgc
    10081 aactttatta atggctgctg cttcttccaa tacagaagtt aaatagttta cagccattga
    10141 atattggcca aaattactgc gttttatgtt ttgaagagac agccctcttt tttcagcaag
    10201 tagaggaaga taccaatttt cttctaaatt gtagagtgct gttttacgtt cttggattgg
    10261 gaatttaaaa cctaatacgt tcatttttat gctcctattt attttctttt tgtcatacta
    10321 gaaaagattg gtctttttgt ccagaataat cttatttttg ggtagctgcc ttattatttt
    10381 gttcgcaaca aaatgagtag taacacaatt attatttaca agaagaacga taagctataa
    10441 tttacccttt ttgttatcta acgagattct acccttcgtt aacataaaca atatcaccgt
    10501 ttatcatcgt taattcactt tttgcagtta accaaaaagt ggaagggtat tgtctaaagt
    10561 cctcaataac gattaaatct gccaatttac ctgtttctat tgatccgcgt ttatgttcac
    10621 tttttgtaga tttagcagct cccattgtat atccccaaat tgcttcgaac ggattgattg
    10681 attgctctgg tctaaattgt ggatttttag gattattatt ttggcgtctt tctatagcag
    10741 agtaaattcc taaaaatgga ttgatagttt ctatgggagc atctgaacca aaggcatatt
    10801 caattcctgt agaaattagg cttttaattg aatatgtttg ttctttaata tcactccagt
    10861 atttattgat tagaggaata tcattggcta tatgcacagg ttgtaacgat gcaaatagtc
    10921 cagaactttt tagcagaggg atatcctcat tacggattgc ctgaatatgt tcaatacggt
    10981 taaaaagggt attatgttta ccagtttttt tcagccgcag gaaacaatca attacttgtt
    11041 ttacacagcg gtttccaatt gaatgaattg tagaagaaag tccattctct gcagctgaaa
    11101 gcactaagga aaataattca tcatctgtgt agcgtaaaat acctttttcc ccttgcggat
    11161 aactttcaaa catagcagct gtttgagagc ctaaactacc atctccaaaa agttttaagc
    11221 caccaagttt ataaaactca tttccttcat agcttttgat accttcctgc aaaacctttt
    11281 cataatcctc tggataaaaa tgccagcaaa aacggaattt ctttctcttt tcttgggttt
    11341 ttaacattag gtctctgctg aaaatgcttt ccatactgtt aaaaccaacc aaacccaaag
    11401 ggtatataga atccaccgtt tctgagatag cttgaataat aacttcagat tccggataga
    11461 caatataggg atctattagt tcattggctg attcataaag aactcctgta agttcaccaa
    11521 tactattatg ctcaattaaa cctcctttag gatttgctgt atctttacaa attcccgcta
    11581 tttttaaagc taaggaattg catagtttac tatgataatc cttactcatt aaagcaacag
    11641 gaatatccgg aaagattgta tccaaaagct taagggatag ttcgtcagga ttatttaagc
    11701 agtttcgatc ccaaccacct cccaaaatcc attttagctt ccagctaaga ttatctctat
    11761 aggatataag gtaattgcgt atttcatcta tagtatggca accgtttaaa tttaccagta
    11821 tgcgtccttt ggcatattct acaaaatgag tatgcgcatc taaaaaagca ggcagtaata
    11881 atctattttt gaggttgatt atttcataag atgattttgc ctgcattttg cattcttgca
    11941 gatttcctat taaggcgatt cggtcattat gaatgagaac ggatttttct tcaaagtttt
    12001 gggtctggct gtttagcaca aatccgttag taaagatgta attcattatt ttatcctaat
    12061 attgctttta tctagctgcc taaagcatag aagcttaggg ttctttaatt tgtaaaataa
    12121 ggaggcattg gtggctccct tattctaata actctcttaa ggtttgttcc aattctacag
    12181 ctttatcagt tttttcgtcc ctatatttaa taataattgg gcaggcaact tgaaattctg
    12241 ggttactttt taaccgtctg gaaccaggca caacaacagc tccataagga atagtaaaac
    12301 tattaccgct atcataattt aaatatcttc cttgtacact atcataaata ggagtggaag
    12361 cagttatcac agttccagaa gctatcactg ctttactttg aacaattata ccttcataga
    12421 tacctgtgtt tcctcctaca aaaacatcgt cttctataat tacaggacgt aaacctacag
    12481 gttctaatac gcctccaatt atagctccag cagacagatg tacatttttc ccaatttgtg
    12541 cacaggaacc aaccaaagaa tgggaatcaa taagtgttcc actatctaca taggcaccaa
    12601 tatttataaa ggcaggaggc ataattgcaa ctccggtagc tatataacaa ccatttcgtg
    12661 ctgaacttcc tccagggaca attctaattc
//
LOCUS       DAORHB010000020        12667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_4085_length_12667_cov_4.327624, whole genome shotgun sequence.
ACCESSION   DAORHB010000020 DAORHB010000000
VERSION     DAORHB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12667
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4085_length_12667_cov_4.327624"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PKM80_01260"
     CDS             <1..1247
                     /locus_tag="PKM80_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNV92566.1"
                     /translation="QLMAITHELMRIVNINELERAIVSACIDFTNSTRGFLIKRDSDG
                     AIIYTVQLNAAKQILPTVSGVSKTVLSLSQNTQEIVSTFNAVEDNRFKSAISVQDYVL
                     HTIFCAPIIVDNNVYGYIYLDNLNDNNREMYLNTEIIKLLLEQITIALKNAMLYENLL
                     QKNNELNTFETLKDEFMAIVSHELNTPLTTLQGYVTRLKRNLFADEEEKKDIINKIEN
                     SVKKIILTINDITTMNTYNLAKSLPFSTLSVQEILEIIYQEVQILSRKRKMFIKLEIE
                     KDLPPIKANWEAFHLMIYNLVLNAIRFTNDFGTVIIGARKSAFQSEKIDGKESLVIYV
                     QDNGIGIPEYQLKNVFRKFYELNEIYAHKSGTIEYRSSGLGLGLATSKRIAELHSGKI
                     WIKSKENEGTTVFVTIPIKQSK"
     gene            1264..4185
                     /locus_tag="PKM80_01265"
     CDS             1264..4185
                     /locus_tag="PKM80_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV92567.1"
                     /translation="MKRYIIMMLCLVLMCICFAESVGKKSSPSLLAVSNSETYAYRYI
                     VELYDAGDAYNLSKEITLFKATYPESVYLPYIRLIEGNLAMESGNYTKAIEIYELLLN
                     IDLVQNVRNDILLNYAYCLSELKQYDKAFNILQRLDSETVNPVYIELSNNLRGDIYFH
                     LGQFYSAEKAYKRAMVAFPDNEGIRFSLFSTYLALDKDDDALELLKAITPESDYYYKY
                     IGSWLGYLLDNELYEDFDRFVEEQKLEDITSNLDIIDIRIRRALHIGDFEKTSQLLQK
                     ITIESPQFQYYQALVLINAGKEKQADSLLALLVSSARPEISVPAYMERVKLLFKKNPK
                     AAIEQLNSYLKQTPSDFMKAELYYTMGFFYYNTGNYSEAIRHLGIARHYETSRELCSR
                     IDILIAEAFYASGNNDLAKDTFNRYLNLYPYGSRRDKAYFYLGYMSFQEKDYTQAKVN
                     FQMLQEQYPNSPYCNDATFYLAEMDFYLANYNLALENYLYLYSKKVDNEVVALRITQV
                     YYYLGDYEKSESYIANLVPSYDTCILKGNIMLVKKEYEKAIDQFLQAEKYAPDNLKTI
                     EAKSYRALCLYQMKHFKEASALYLQLSGQKESPDTYLFLAAKSAYAAKDYHLALELYN
                     SFIDKYPESTHFLEALSNIANTYYNMGNYDRAINDWINILTRFRNQTKFTDNQLATIQ
                     DALIGLELALQYVNKEDLLAELLVLPDTFFSEYIKFELNYILVKVYANKKLWADLIAS
                     AEKMKEDFPQYRQEDVELLMATALIELNEYTKADSLLSELYAESRNNDTLLKWAELEF
                     ITGNYESALEKYKLAYKNNPNSEIWVNMLKCSEANNYNEFDHLWTLGIDFLENCPEVN
                     VFRIRQLYYTEHFEEANQLAEHLINYSLSTFDHATAFLVMGMINYRKGEYQTAISTLS
                     KVVMLFPEHKDICSKAINYIVRSLLDSGAYTEAEAFFIQHENELTITDKTELTELLEG
                     I"
     gene            4185..5663
                     /locus_tag="PKM80_01270"
     CDS             4185..5663
                     /locus_tag="PKM80_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92568.1"
                     /translation="MKRFFLTLASILTLSLLIAQTQELPDLTISGESSFKPYLHKHSL
                     LFDHSLVMGDSLPAFIPPAVSTNENRSESYTMQHRCYLQFEGNVDYGFNSFISYYPTS
                     EVLNALTYTLDMCSPVSKLLSVHNKLFLGTEFSSKLPLSFRLQNVSSHADDFDNTLLD
                     FTVSHHRPIVSIGDFILKDISAQLGYSYLYQQNLGSSYKGNYLNLYLASRLDVDPVDI
                     KAKILTREGDVGFQLSTNAKDEYMNISRLGVSVLADEYTFIPSLEFLYRSPIQKFGVV
                     SIANEPLLESNTFLSHMEENPWIAFDDGHKLKKIPLNFKLGLEYLYPEKMNFTLAYLT
                     IKNNSRYEIDSPVLINSINYSIPTVRYTDIFSNLTIIEAFFKLDEVNIHQGMELDLSY
                     LSEDDYRRAPYRPLLNLDSRFSYNFPRWFFSLDILQKYFTVNHLGNDLPEAVIINIGA
                     EYREANYAVYFELANLFNRKQYVFSELPAQGRNFYLGLKHRF"
     gene            5729..6568
                     /locus_tag="PKM80_01275"
     CDS             5729..6568
                     /locus_tag="PKM80_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92569.1"
                     /translation="MKLKVLNLIREILNVLFGDYNGIQVFIAPVTLVYWIDSNSLITS
                     ATSLISFRIHYLPLLAFFTILAFVVFMLVKIKLLYPCSGSEFIDLTIQLNVSVMALIL
                     IALIIYALSNFLAYFYGIKGTVKSGLYLLFKLYTAILILYHYLINVVLAPFYRRRYSH
                     LHALKNFLVWARHNKFLLFRYVLLIVMVIFFAVRVYQIVLQFLIIPAINSISDFTGFD
                     LKFKLYPFLGVSDIFTNILAIFGAFTVSNLLFFPIIWVLKYLVDRFLPFSNLIRSNYA
                     QIT"
     gene            6552..7841
                     /locus_tag="PKM80_01280"
     CDS             6552..7841
                     /locus_tag="PKM80_01280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HNV92570.1"
                     /translation="MPKSHKKKLRKIKRRRKNNNLGVFILVGFLACFIIFVIIREPIN
                     EQVGKKTDTVDQKQDMPSRSKKRNKKEGKILTHDKKSTSVSDRKALGTNQIREEKNTP
                     EVAIVNTIGKLGIPKSYYRKRNTEEYTIYNVPIDKSIMDLVYANMIFKGELESNGAKF
                     IKGTDNPGKQTLLFNFPNVPKQYQINIYYDSELYKNKQTRRTITLVVDDFGPVGGDLL
                     EGFLALDKEICFAILPDETYSVLTMEKAKEQGRTTIIHIPMEPIGYPEVNPGKNAILV
                     QYEDRQIDKVLSHFIEQLPYCEGVNNHMGSLATTDEEVMSAVMATLKKHNKFFLDSRT
                     TNISIAYAIAQKYHIKSYRNDLFLDSPNISQSTMDAKLNQIIELSNNNKNVIAISHCH
                     NFEKLQYLKRIINRLKAAGFTLVPITEIDKYNVPEIL"
     gene            7851..8666
                     /locus_tag="PKM80_01285"
     CDS             7851..8666
                     /locus_tag="PKM80_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNV92571.1"
                     /translation="MSFFQAIIMGIIQGLTEFIPVSSSGHLVLFQYFFGMGESENIVF
                     ELFLHLGTLFAVLIYFRKTLWELFKSLFCWGNTVNREQHRKNRTLVYYLIISTLATAI
                     IYILVGDYFKAAFAMPILVAFFLLITGGIVFLSDYFVDQGIPASNVGFFRAVIIGIGQ
                     GIAILPGVSRSGTTIAYSLATGMKRKDAAQYSFLLSIPAILAGNLSEYKALTELDSTM
                     LQNYLVGFVSSFIIGYLVIAFLIRLIENSRLKYFGIYCWIAGLLSIILLALGI"
     gene            8666..9667
                     /gene="holA"
                     /locus_tag="PKM80_01290"
     CDS             8666..9667
                     /gene="holA"
                     /locus_tag="PKM80_01290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNV92572.1"
                     /translation="MEAPIFALDFAAANVNLGSSYLLVGTDSYLVDKVIETIKKKLKK
                     KENIDTFIIYGDDVKSSELADILDTYTIFSSAKLVILKNAEQLNKYELKTLSSYFSSP
                     SEIQSLAIATEKIDLHNESWKQIKAHSTIINCESPRYGGAIRSWLDKSLKDMNKTMTI
                     KAIEEFINRIELDYYYAANELNKLDLLTTGRNRITEEDVMKSLGTTRTGTLIDFYRAL
                     GKKQEKQAIEALEKMLNANWEFLQIFFQFTKFYNNIWHILLLKKAHISDNEIVSKHLS
                     DIYPNQRKEFLELSRSYNLASLEKIFTILLETDQQFKLSVADSQILLITCLIRVMEA"
     gene            9667..11130
                     /gene="malQ"
                     /locus_tag="PKM80_01295"
     CDS             9667..11130
                     /gene="malQ"
                     /locus_tag="PKM80_01295"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNV92573.1"
                     /translation="MHKTGILLHISSLPSDFGIGDFGPEAIRFAEYLKTEGYTYWQIL
                     PITHCGYGNSPYNPLSAFALNPYFISPELLYQQGLINQRMLKAVKIPASNITQYERVY
                     NLKDKMLAQATDNWMLTNNLDDYIEENAFFLKPYLTFLSLSRIYDDSCWYNWNEEHKH
                     YSEALYQKTRQLDSSFINIKAATQAMLSEQLKYFKEYLHKMGIILVGDLPLYLSYDSS
                     EVWAHPEFFDLDEQGRRLHFAGVPPDAFSEKGQLWGNPLYKWDVLQQDSFKLFMNRIG
                     MALSYLDILRLDHFIGYVNYWQVKPNFDEKGHPILPQNAINGSWEPALPEEFFSTLLK
                     NYGIERFLAEDLGILNQEVCRVRDSFGFPGMIILQFCFEDSVPEVKKYPPERWLYTGT
                     HDNHTIRGWFESLPSDSLSLKHLQEYCIQNKITDIHQMINSENIHNIMRNIALSSGCQ
                     QIIFPLQDILGLGDEARMNVPGTALGNWQWRLEERIY"
     gene            11158..>12667
                     /locus_tag="PKM80_01300"
     CDS             11158..>12667
                     /locus_tag="PKM80_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV92574.1"
                     /translation="MPKLMLLGDEALAQGALDAGISGFYGYPGTPSTEIIEYAQKSKQ
                     AAENNIHRTWSSNEKTALEAALGMSASGKRAMVTMKHVGLNVAADPLMNSAITGASGG
                     LIIAVADDPSMHSSQNEQDSRYYGNFAMIPVLEPGNQQECYDMAFYGFELSEKYHIPV
                     LLRLTTRLAHSRSGIVTKEPIPQNEMRKPEDMFQFMLLPAIARKKYQHHLELQKDFMR
                     ESLNSPFNAYKTEASSKKLGIITTGLAYNYLREAFKGEEIPYPVLKIGQYPLPEKQIH
                     QLYDSCDELLVIEEGMPVVEEQLKGLAFPGLKPIHGRLDGYLPRTGELNPNLVAKAFS
                     LPDTIGRPVPEIVVGRPPALCVGCPHSDTFLSLNEVMAEYGRGHVFSDIGCYTLGFMP
                     PYNSINSCVDMGASITMAKGASDSGLFPAVAVIGDSTFCHSGITGLLDCIYDKANVMI
                     VILDNATTAMTGGQNYTGYGKLEDICLGLGVEKEHIRIFVPLKKNYPEITQIY"
BASE COUNT         4157 a         2080 c         2318 g         4112 t
ORIGIN      
        1 accagctgat ggctataaca cacgaattga tgcggattgt aaatataaac gaattggaaa
       61 gagccatagt atctgcctgc attgatttta ctaatagtac aagaggtttt ctgattaagc
      121 gtgatagtga tggagcgata atatataccg tgcagctcaa tgcagcaaaa caaattctgc
      181 ccacggtatc tggagttagt aaaactgttt taagtttgtc tcaaaacact caggaaattg
      241 tctccacttt caatgccgtg gaagataatc gttttaagag tgcgataagt gttcaggatt
      301 atgttttgca cacgatcttc tgtgctccca taattgtaga caacaatgtg tatggctata
      361 tctatctgga taacttgaat gataataacc gcgaaatgta tctgaataca gaaataataa
      421 agctgctatt ggaacaaatt acaattgctc tgaaaaatgc tatgctctat gaaaacttac
      481 tgcaaaagaa taacgagcta aatacatttg aaacgctgaa agacgaattt atggcaatag
      541 tatctcatga attaaataca ccgctaacaa ctttgcaggg atatgttacg agattaaaaa
      601 gaaatctttt tgctgatgaa gaagaaaaga aagatattat caacaaaatt gagaattcag
      661 tgaagaaaat cattctcact atcaacgata ttactactat gaatacctat aatttagcca
      721 agtcgttgcc cttttccact ctatctgtgc aggaaatatt ggaaattatc tatcaggaag
      781 tccaaattct ttctcgtaaa cgtaagatgt tcataaaatt ggaaattgaa aaagacttac
      841 ctccgattaa agcaaattgg gaagcgtttc atttaatgat ttataatctg gttctaaatg
      901 ccattcgttt taccaacgat ttcgggactg tgattattgg agctcgtaaa tctgcttttc
      961 aaagcgaaaa gattgacggt aaagagtcat tagttattta tgtacaagat aatggaatcg
     1021 gaattccgga ataccaattg aagaatgtat tcagaaaatt ctatgaactg aacgaaatct
     1081 atgctcataa atctggaact atagaatatc gtagtagcgg tttgggtctt ggactggcta
     1141 catccaaacg cattgctgaa ttgcattctg gtaaaatctg gataaaaagt aaggaaaatg
     1201 aaggaacaac tgtatttgtt acaataccaa taaaacaaag taaataggtg aaataaacaa
     1261 cttatgaaac gatatattat aatgatgtta tgccttgtct tgatgtgtat ttgttttgct
     1321 gaatctgtag gaaaaaagag cagcccttct ttgttggcag taagtaatag cgaaacatac
     1381 gcatatcgtt atattgtaga actttacgat gcaggtgatg cttacaatct gagtaaagaa
     1441 attactctgt ttaaagctac ctatccagaa tcggtatatt taccctatat aaggttgatt
     1501 gaaggtaatc ttgctatgga aagtggaaat tacactaaag cgatagaaat atatgaactc
     1561 ttactgaata tcgaccttgt tcaaaatgtg cgtaatgata ttttgttgaa ttatgcttat
     1621 tgcctgagtg agttaaaaca atatgataaa gcattcaata tattacagcg tttggatagt
     1681 gaaacagtaa atccagtgta tatcgaatta tcaaataact tgagaggaga tatatatttt
     1741 catttagggc agttctattc tgccgaaaag gcatataaaa gagcaatggt agcatttcct
     1801 gataatgagg gtatccgttt ttccttgttt tctacttatt tggctttaga taaagatgat
     1861 gatgccttgg aactgctgaa agcaataact cctgaaagtg attattatta taaatatata
     1921 ggtagctggc tgggatatct attagataat gaactttatg aagactttga tagatttgtt
     1981 gaagagcaga aactagagga tatcacttct aatctggata ttatagatat cagaattcgc
     2041 agagctttgc acataggtga ttttgaaaaa acatctcaac ttctgcaaaa aattacaatt
     2101 gaatctcctc agtttcaata ttatcaggca ctggttctga taaatgcagg taaagaaaag
     2161 caagctgatt ctctattggc tttgcttgtt tcctctgcta gaccagaaat ttctgttccg
     2221 gcttatatgg aaagggtaaa actattattc aaaaagaatc ctaaagctgc aattgagcag
     2281 ctgaacagct atttgaaaca aactcccagc gactttatga aagccgaatt atattacact
     2341 atgggctttt tctattataa tacaggtaac tattctgagg caatcagaca tttaggaata
     2401 gctcgccatt atgaaacaag ccgcgagctt tgtagtagaa tagatattct gatagcagaa
     2461 gcattctatg catctggaaa taacgatttg gctaaagata cattcaatcg ctatcttaat
     2521 ctttatccat atggcagcag aagagataag gcatatttct atcttggcta tatgagcttt
     2581 caggaaaagg attataccca agccaaggtt aactttcaaa tgcttcaaga gcaatatcca
     2641 aattccccct attgtaatga tgcaactttc tatttggcag agatggattt ctatttagca
     2701 aactacaatc tagcattgga aaattacctt tatttgtaca gtaaaaaagt agataatgaa
     2761 gtagttgctt tacgcattac acaagtatat tactatctgg gagattatga aaagagtgaa
     2821 agttatatag caaatttagt tcctagctat gatacctgca tcttaaaagg gaatattatg
     2881 ctggtaaaaa aggaatatga gaaagctata gaccaatttt tacaagcaga gaaatatgct
     2941 ccagataatt taaaaacaat agaagcgaag agctatcggg ctttgtgctt atatcagatg
     3001 aaacatttta aagaagcatc agctttgtac ctacaacttt ctgggcaaaa agaaagccca
     3061 gatacatatc ttttcctagc tgctaaatcg gcttatgctg ctaaagatta tcatttggct
     3121 ctggaattat acaattcttt tatagataaa tatccggaat caacgcattt tttggaagcg
     3181 ctttccaata ttgccaatac ctattataat atgggtaatt acgatagagc tattaatgac
     3241 tggataaata tcttaaccag gttcagaaac caaacaaaat ttaccgataa ccaactggca
     3301 actatacagg atgccttaat tggtttggaa ttggctttac aatatgtaaa caaagaagat
     3361 cttctagcgg aattgctagt tttgccagat acttttttca gcgaatatat caaattcgaa
     3421 ttgaattata ttctggtgaa agtttatgca aacaaaaaac tctgggcaga ccttattgcc
     3481 agtgccgaga aaatgaaaga ggattttccc caatataggc aagaagatgt ggaattgttg
     3541 atggctacag ctcttattga attgaacgaa tatacaaagg cagattcatt gctatccgaa
     3601 ttgtatgctg aaagtagaaa taacgatact ttactgaaat gggcagaact cgaatttatt
     3661 acaggaaatt atgaatcagc tttggaaaaa tacaaactag cttataaaaa caatcctaat
     3721 agtgaaattt gggttaatat gctaaaatgt tcagaagcga ataattataa tgaatttgat
     3781 catctatgga cactaggaat agactttctg gaaaattgcc ctgaagtaaa tgttttccgc
     3841 attcgccagc tctattatac tgaacatttt gaagaagcaa atcagcttgc agaacacctt
     3901 attaattata gtttatccac ttttgatcat gctacagctt ttttagtgat ggggatgatt
     3961 aattacagaa aaggagaata tcaaacggca atttctacct taagtaaagt ggtaatgctc
     4021 tttccagaac ataaagatat ttgtagtaaa gcaataaact atatagttcg ttctcttctt
     4081 gattcgggag catatacaga agcggaagcg ttttttattc aacatgaaaa cgaattgacg
     4141 ataactgaca aaacagaatt gacagaactt ttggagggaa tataatgaaa cgcttctttt
     4201 taactcttgc ctcaatcctt actctttcat tattgatagc tcaaactcag gagcttcccg
     4261 atttaacaat tagcggtgag agttccttta aaccctatct acataaacat tctttactat
     4321 ttgaccattc acttgttatg ggggatagct tgcctgcttt tattccccca gctgtttcta
     4381 ctaacgaaaa tagaagcgaa agctatacta tgcagcatcg atgttatctt caatttgaag
     4441 gtaatgtaga ttatggattc aatagtttca tcagttatta tccaaccagt gaagtgttaa
     4501 atgctctaac ctatacttta gatatgtgct ctcctgtttc gaaactgtta tctgttcata
     4561 ataaactctt cttagggacg gaattcagtt ctaagcttcc tctctctttt cggctccaaa
     4621 atgtttcctc gcatgctgat gattttgata atactctttt agattttact gttagccatc
     4681 atagacccat tgtttccata ggagatttca ttttaaaaga tatttctgca caacttggat
     4741 atagttatct ataccagcaa aacctgggta gttcttataa aggtaactac ttaaatttat
     4801 atcttgcctc acgcttggat gttgatccag tggatataaa agcgaaaatc cttaccagag
     4861 aaggggatgt aggattccaa ctatctacaa atgctaaaga tgagtatatg aatatttcca
     4921 gattaggggt tagtgtatta gctgatgaat atactttcat cccttcatta gaatttcttt
     4981 atcgttctcc tattcaaaag ttcggtgtag tgagtattgc taatgaaccg ctgttagaaa
     5041 gcaatacttt tctttcccat atggaagaaa atccctggat agcttttgat gatggacata
     5101 agctaaagaa aataccgctt aacttcaaat taggtttaga atacctatat cccgaaaaaa
     5161 tgaatttcac tttagcctat ctaaccatca agaataatag tcgttacgaa atagacagcc
     5221 ctgtacttat caatagtatc aattattcta ttcccactgt tcgttatacg gatatattct
     5281 ccaatctaac tataatagaa gcgtttttca agctggatga agttaatata catcagggaa
     5341 tggaattaga tctatcctat ttgagcgaag atgattacag aagagcaccc taccgtcctc
     5401 ttctaaactt ggattctcgc ttttcttaca attttcccag atggtttttt agtttggaca
     5461 tcttgcagaa atactttact gtaaatcact tgggtaatga tctacctgaa gctgtaatta
     5521 tcaatatagg tgcagaatat cgtgaagcaa attatgcagt ctattttgaa cttgctaatc
     5581 tctttaatcg caagcaatat gttttttcag aattacctgc acaaggtcgt aatttttatc
     5641 ttggtttgaa acacagattt taggatttta ttgacaaaat accctatgta taggaataaa
     5701 gaatacaatg ttaaaatgaa ggtaaataat gaagttaaag gtgcttaatc taatacgcga
     5761 aattcttaat gtcctgtttg gagattataa tgggatacag gtatttattg cacctgtaac
     5821 tcttgtatat tggatagata gcaatagctt gatcacttct gcaaccagtt tgatcagttt
     5881 tcgaattcat tatcttcctt tacttgcttt ttttactatt ctggcgtttg tcgtttttat
     5941 gttggtaaaa attaaattac tatatccttg ttccggtagt gagtttattg atttaacaat
     6001 tcaattgaat gtgagcgtaa tggctttaat tcttattgca ctgattatat atgctttatc
     6061 aaatttcttg gcttatttct atgggataaa aggaacggta aaatctggat tatatctgct
     6121 tttcaaacta tatacagcaa tattaattct ttatcattat ctcattaatg tggttttagc
     6181 tcctttttat agaagacgat acagccattt gcatgcgctt aagaactttc ttgtttgggc
     6241 aaggcataat aaatttctgc tttttcgcta tgttttattg atagttatgg tgatcttttt
     6301 tgcagttcgt gtataccaaa tagtcctaca atttcttatt ataccagcaa tcaatagtat
     6361 tagcgatttt acgggcttcg atttaaaatt caagttatat ccatttttag gagttagtga
     6421 tatttttaca aatatactgg ctatatttgg tgcttttacg gtctccaatt tactcttctt
     6481 tcccataatc tgggtattaa aatatttggt agaccggttt ttgccttttt ccaatctaat
     6541 aaggtctaat tatgcccaaa tcacataaga aaaaactgcg taaaattaaa cgccgcagaa
     6601 agaacaataa tctaggcgta tttattcttg tgggattctt ggcttgcttt atcatttttg
     6661 tgataattag agagccaatc aatgaacaag ttgggaagaa aacagatact gtagaccaga
     6721 aacaagatat gccttcaaga tctaagaaaa gaaataaaaa agaaggtaaa atccttacac
     6781 atgacaagaa aagcacttct gtatcagata ggaaagcact tggaacaaat caaatacgag
     6841 aagaaaaaaa cacgccggaa gtagctattg ttaacacaat tggcaaattg ggtataccca
     6901 aaagctatta ccgtaagcgt aatacagagg aatatactat atataatgtt cctattgata
     6961 aatcgattat ggacctcgtt tatgctaata tgattttcaa aggcgaattg gaaagtaatg
     7021 gagctaaatt catcaaggga acagataatc ctggtaaaca aaccctacta tttaatttcc
     7081 cgaatgttcc taaacaatat caaataaaca tatactatga ttctgaacta tacaagaata
     7141 aacagacaag acgcacaatt actttagtgg tggacgattt tggtcccgtc ggtggtgacc
     7201 tgcttgaggg ttttctggct ttggataaag aaatctgttt tgctatatta ccagatgaaa
     7261 cttacagtgt tttaacaatg gagaaagcaa aagaacaggg acgaactacc attatccata
     7321 ttcccatgga accaattggt tacccagaag taaatcctgg taaaaatgcc attttagttc
     7381 agtatgaaga tcgtcaaatt gataaagtcc tttctcactt tatagagcaa ttaccttatt
     7441 gtgaaggagt aaataatcat atgggcagtt tggctactac agatgaagaa gttatgagtg
     7501 ctgttatggc aactctgaaa aagcataaca agttcttttt agatagccgt actacaaata
     7561 tctcaattgc ctatgcgata gctcaaaagt atcatatcaa atcttatcgt aatgatctat
     7621 ttctcgattc tcctaatatc agtcaatcga ctatggatgc aaaattaaat caaattattg
     7681 aactttccaa caacaacaaa aatgttatag ctatatccca ttgtcataat tttgaaaagt
     7741 tgcaatatct taagcggata attaatcgtc ttaaagctgc tggcttcact ttggttccta
     7801 tcacagaaat tgataaatac aatgttcctg aaatattata gaggtatatt atgagttttt
     7861 ttcaagctat tattatgggt attatacaag gattaacgga atttatcccg gtaagcagtt
     7921 cggggcatct ggtattattt cagtatttct ttggtatggg agaaagcgaa aatatagttt
     7981 ttgagctctt cttacatttg ggaactttgt ttgcagttct tatttatttc cgtaaaactc
     8041 tatgggaatt attcaaatct ttattctgct ggggaaatac agtgaaccgt gaacagcatc
     8101 gtaaaaacag aactttagta tattatctaa ttatctcaac cttggcaacc gctataatat
     8161 acattcttgt tggtgattat tttaaagctg cttttgcaat gccaatacta gtagctttct
     8221 ttttattaat taccggcggc atagtttttc tttccgatta ttttgttgat caaggtattc
     8281 ctgcttcaaa tgttggtttc tttcgtgctg tgataattgg tataggacag ggtatagcta
     8341 tattacctgg agtttcacgt tcgggaacta ctattgctta ttcgttggca acagggatga
     8401 agcgtaagga tgcagctcaa tattcttttt tattaagcat tccagctatt ctagcaggta
     8461 acctttctga atataaagca cttactgagt tggattctac tatgctgcaa aactatctgg
     8521 ttggctttgt atcttctttt ataattggct acctggtgat tgcattttta atcagattaa
     8581 tagaaaatag ccgtctgaag tattttggaa tatattgttg gattgcaggt ttgctctcta
     8641 ttatccttct ggcattaggt atataatgga agcacctatt ttcgctcttg attttgctgc
     8701 tgcaaatgtt aatttgggct cttcctatct tcttgttggg acagattcct accttgtgga
     8761 taaagtgata gagacaatca agaaaaaatt gaagaaaaag gaaaatatag atactttcat
     8821 tatttatggt gacgatgtta aaagctcaga actggcagat attttggata cttacacaat
     8881 tttctcttca gcaaaacttg tgatcttgaa aaatgcagaa caattgaaca aatatgaact
     8941 gaagacctta tctagctatt tttcttcacc ctccgaaatt caaagtttag ctatagcaac
     9001 ggaaaagata gacctgcata atgaatcctg gaaacaaatt aaagctcatt ctacaataat
     9061 taattgtgaa tctcctcgtt atggaggggc aattcgttcc tggttggata aaagtttgaa
     9121 agatatgaat aaaacaatga ctatcaaagc tattgaagaa ttcataaacc gcattgaact
     9181 cgattattat tatgctgcta atgaattgaa caaactggac cttttaacta ccggcaggaa
     9241 tagaattaca gaagaagatg ttatgaagag tttaggcact acccgtactg gaactttaat
     9301 agatttttat cgggctttag gcaaaaaaca ggaaaagcaa gctatagaag cattggaaaa
     9361 gatgctgaat gccaattggg aatttttgca aatattcttc cagttcacga aattttataa
     9421 taacatctgg catatattgc tgctgaaaaa ggcacatatt tccgataatg aaatcgtatc
     9481 caaacatctt agtgatatat atcctaatca gcgaaaagaa tttttggaat tgtcccgttc
     9541 ctataatcta gcttcgctgg aaaaaatctt caccatttta ctggaaaccg atcaacagtt
     9601 taaactttcg gttgctgatt ctcaaattct tctgattact tgtttaatta gggttatgga
     9661 agcataatgc acaagacagg tatcttattg catatcagct cgttgccttc agatttcggt
     9721 atcggagatt ttggacctga agcaattcga tttgcagaat atttaaaaac agaagggtat
     9781 acttattggc agatattacc cataactcat tgcggatatg gaaattctcc ttataatcct
     9841 ctttccgctt ttgccttaaa tccctatttt atcagtccgg agctgcttta tcaacaaggc
     9901 ttaatcaatc aaagaatgct gaaagcagta aaaatacccg caagtaatat cactcaatat
     9961 gaaagagtat ataacttgaa agacaaaatg ctagctcagg cgactgacaa ctggatgcta
    10021 accaataatc tggatgatta tatagaagaa aatgcctttt ttttaaagcc ctatttaact
    10081 tttctatcgt taagcagaat ttatgacgat agctgttggt ataattggaa tgaagaacac
    10141 aaacactata gcgaggcctt atatcaaaaa actcgtcaat tggattcctc cttcataaat
    10201 atcaaagcgg caactcaggc aatgttatcg gaacagctga aatactttaa agaatatttg
    10261 cataaaatgg gaataattct cgttggcgat ttaccgcttt atctttccta tgatagttct
    10321 gaggtttggg ctcatcctga gttctttgat ttagacgaac agggaaggcg tttacatttt
    10381 gcaggtgttc ccccagatgc tttttcggaa aaaggtcagc tttggggtaa tccactttac
    10441 aaatgggatg ttttacagca ggatagtttt aagcttttta tgaatagaat tggtatggcg
    10501 ctaagttatc tagacatttt gcgactggat cattttatcg gttatgtaaa ttattggcag
    10561 gtaaagccaa attttgacga aaaaggacat ccaatacttc cccaaaatgc cataaacggt
    10621 tcttgggaac ctgctttacc agaagagttc ttctccactt tattaaagaa ctatggtata
    10681 gagcgttttc ttgcggaaga tttaggtata ctaaatcagg aagtatgcag agtaagagat
    10741 agttttggtt ttccaggaat gattatttta caattttgct tcgaagatag tgtacctgaa
    10801 gtaaaaaaat atcctcctga acgttggctc tatactggaa ctcatgataa tcatacaatt
    10861 cgtggatggt tcgagtcgct tccttctgat tcactatcat taaagcactt gcaagaatat
    10921 tgcatacaaa ataaaataac tgatatccat cagatgatca atagcgagaa tatacataac
    10981 ataatgagga atattgcctt atcttcaggt tgccaacaaa tcatatttcc acttcaagat
    11041 attcttggtt taggtgacga agcaagaatg aatgttcctg gtactgcttt aggtaattgg
    11101 caatggcggt tagaagaaag aatttattaa ttataaataa aataaaaagg agaaaaaatg
    11161 ccaaaactta tgcttttggg tgatgaagcc cttgctcagg gagctttaga tgccggaata
    11221 tcaggatttt acgggtatcc gggaactccc tcaactgaga tcatcgagta tgcacaaaag
    11281 tcaaaacaag cagcggaaaa caatattcac agaacctgga gttcaaacga aaaaactgct
    11341 ttggaggctg ctttgggtat gagtgcttcc ggaaaaagag ctatggtaac tatgaaacat
    11401 gtaggattaa atgtggcagc agatccttta atgaattctg ctatcacagg agccagtggt
    11461 ggtttgatta ttgctgtggc agatgatcct tctatgcaca gttctcaaaa tgaacaggat
    11521 agccgttact atggaaattt tgctatgatt cctgttttgg aaccaggaaa tcagcaggag
    11581 tgttatgata tggcttttta cggattcgaa ttatccgaaa agtaccatat acctgtgctt
    11641 ttaaggttaa caacgcgtct agcacattcc cgttcaggga ttgttacaaa agaacctatc
    11701 cctcaaaatg aaatgcgcaa acctgaggat atgtttcaat ttatgcttct accggctatt
    11761 gccagaaaaa aatatcagca tcatttggaa ttgcaaaagg attttatgag ggaatcctta
    11821 aattcacctt ttaacgctta taaaacagaa gcgagctcaa aaaagttggg tataattaca
    11881 actggcttag catataacta tctgcgagaa gcttttaaag gtgaagaaat tccctatccg
    11941 gttctgaaaa taggtcagta tcctttacct gaaaaacaaa ttcatcagct ctatgatagc
    12001 tgcgatgaac ttctagttat tgaagaaggt atgcctgttg tggaagaaca acttaaaggc
    12061 ttggcttttc ctggtctaaa accaattcac ggacgtttgg atggttacct tcctcgtact
    12121 ggtgaactta accctaatct tgtagctaaa gcattttctc tgcccgacac aataggtaga
    12181 ccagttccag aaattgttgt gggtagacct ccagctttat gtgtcggttg tcctcattcc
    12241 gatacttttc tttctttgaa tgaggttatg gctgaatatg gaagaggtca tgtttttagc
    12301 gatattggtt gttacactct tggatttatg cccccatata attctatcaa ttcttgtgtg
    12361 gatatgggtg cttctattac tatggctaaa ggtgcatctg atagtggact tttcccagcc
    12421 gttgctgtta ttggagatag cactttctgc cattccggaa taactggatt gctggattgc
    12481 atttatgata aagccaatgt tatgatcgta attttggata atgccacaac tgctatgacg
    12541 ggcggacaaa attatacagg atatggaaaa ctggaagata tttgtttggg tttaggagtg
    12601 gaaaaagaac atattcgaat ctttgttccc ttaaagaaaa attatcccga gattacccaa
    12661 atctata
//
LOCUS       DAORHB010000021        12066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_4412_length_12066_cov_5.696861, whole genome shotgun sequence.
ACCESSION   DAORHB010000021 DAORHB010000000
VERSION     DAORHB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4412_length_12066_cov_5.696861"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..348)
                     /locus_tag="PKM80_01305"
     CDS             complement(160..348)
                     /locus_tag="PKM80_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92575.1"
                     /translation="MKDKKEIAAIGAVLALLQSEETYEQYKEPVRGIPSQPYTAYGRQ
                     LTMQYRDLTQRRVIKRSR"
     gene            complement(370..1923)
                     /locus_tag="PKM80_01310"
     CDS             complement(370..1923)
                     /locus_tag="PKM80_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNV92576.1"
                     /translation="MQSTKDAIQGLLEKEKKILQMGGDKAVAKQKQGGKLTARERIDL
                     LFDEGTFRELDMFVSHRCDNFGMEKIEIPSDGVITGHGLVDGRPVFAFSQDFTSRGGS
                     LGEMHAAKICKVMDMALKSGVPLVGIQDSGGARIQEGVDALKGYGDIFFRNSRASGVI
                     PQITAIMGPCAGGAVYSPAMTDFVFMVKNTSFMFITGPDVIRTVTGEDTNQEDLGGAM
                     AHNSKSGNAHFACESDADAIEQIKVLLSYLPSNNMEEPPRIKTNDDPGRLCPELDSII
                     PDDPKMSYDMRDVIHSVVDDGIFFEPHYYYAQNVIIGFSHLNGRTIGIIASQPAFLAG
                     CLDIDASDKATRFVRFCDAFNIPLITFVDVPGYLPGTHQEWNGIIRHGAKLLWCYSEA
                     TVPKLTVVTRKDYGGSYIAMSSRHLGADMVFAWPTAEIAVMGAQGAANIIFRKEIASA
                     EDQEAKRQEMIKIYEEQFNNPYVAASRGYVDAVIVPSETRKRLIDALEILCTKSESLP
                     PKKHGNIPL"
     gene            2373..5570
                     /locus_tag="PKM80_01315"
     CDS             2373..5570
                     /locus_tag="PKM80_01315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV92577.1"
                     /translation="MARLFAKDYHNKSTSWYFNQAVIVLEKDDLWFWKTRNEEGNYQI
                     RFFPVPQKREYFTEYVDIVFNPETELIISHNCSQCNTDESCRHYLSVLRYAYLYLSTR
                     IFEEDTVETCDGNALAGDELWLNLARNAKLYVEGIYNPETDKIRFYHNELSPLDPVLI
                     GKLYAQEEITDISERQVEYYLSNLSAFWDEELHLFSFLNKNRAAYSRKSKFWSIYKKD
                     FAAALLIMQKCRTRFIVKETGEELIFQEQTYPLALRIEPAGTNNYSLRAVVVDELSVW
                     FTGNPSWLFFRNRIYKIYLPFRKEVVDNIFSSGEILSTRDLVYYRCIVHRELHEHNIY
                     LDFEDSIVLPEIVDETPLKRLHIKKLGDKVLVGGSLAFSGEREIPLSVLRFQKPLIHS
                     KYVSPAGEGNAWFHLSPELYEKTQELLEKLPEPELNRLEEYAELVFSGEERIEQLRTA
                     IFNLSDEDWDIQIDPELQNYFISKIPLQVEIIARTDEDIDWFTYEVRYRYRDLNFTHE
                     ELKRFFRSKEEFLHTAEGRIVFISNPQVFYEVEELISHSEQKADKVYRSRLMNLPYYL
                     RLREENPAFRMLGDEWLEGLYDALLNRHLKTFESLPLYLQTVLRGYQKAGVAWIKMLA
                     HYHLNGILADEMGLGKTIQALSAILSTTAGKVSLVICPKTLLYNWAAEIDKFHTNIPY
                     AIVDGNKETRIEILSNPNVRLFIMSYSMVLGDIAYLKNMEFEWIVLDEAQNIKNVSAQ
                     RTSAIKKLNSKHRLALTGTPIENNLTELWSIFDFLNPGYLGTLNKFKQNYLPAEGEIK
                     ARLSLQKMVAPFLLRRVKKEVLLELPDKQEQISWCKLNTLQEKLYLQILDMVHKKLLP
                     EGQEMQSYIHILAALTKLRQVCNHPHLANSDILAELEASSKLEQLLELVTEATNAGHK
                     VLVFSQFVQMLSIIRKVFDAHSLSYSYLDGQTKDRVTPIKRFETNPDIKLFLISLKAG
                     GTGLNLTAADTVILYDPWWNPMVENQAIDRTHRIGQTQKVQVFRLITKGTVEEKILQL
                     QESKRELFDTVIEGGQNMIKAMTKEDIKSLFSYSG"
     gene            complement(6180..7160)
                     /locus_tag="PKM80_01320"
     CDS             complement(6180..7160)
                     /locus_tag="PKM80_01320"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNV92578.1"
                     /translation="MLSIFSPSNYAPFNIACEEYILRDFSEDVFLLYINDSSIIVGKH
                     QNTLAEINYEWVLKHNIPVVRRLTGGGTVFHDSGNLNYSFMVKENGDMNRSFERYTKP
                     ILAILHDLGVPAILEGRNDLTINGCKFSGNAKTVAFGKTLQHGTILFSSNITNLSAAL
                     NPREEKFNDKAVKSVQARVTNVSDHLPYPLSLQDFVTLIRAKVKIMYPEIQDYFLSLK
                     DKEEIQALMNSKYDTWQWNFGKSPRYNLSHSIKTQAGVIEFFLYVNKGIIEEVSIYGD
                     FFTNREISELENALRGIEHKPDTVTKVLQQMDYQSYFGEVNLDELLSAMF"
     gene            complement(7162..8883)
                     /locus_tag="PKM80_01325"
     CDS             complement(7162..8883)
                     /locus_tag="PKM80_01325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNV92579.1"
                     /translation="MFITDEKLKQMPYENLPVDFDVSSWEQVQAFLEELVQHDVSTID
                     NLETMLLKYSDLLKCMADELSWRYIKMTVNADDETKEQAYNDYFANIYAPAEAYQFKI
                     KKRFYESPVRTELESRKYSLLNQIFSNDIKLFREENIPLMIQESELANKYAGIVSKMS
                     AIFEGEEKTPAQLSVYLKDPDRNKRENAWRLRMNMFAEKQEELNKLYSDLIALRHQIA
                     VNVDFSNYRDFKHQEMGRFSYTPEELFAFHKSVEKVVMPFIAEQNKKRKEALKLDTLR
                     PWDTAVDLDGRILKPFETTEEFINKSLKVLYQVNPEYAMQLEKMKNTGLLDLENRKGK
                     APGGYNSSINNLASSFIFMNYVKIHNDIVTLLHESGHAMHSAAMANIKIAQYLETPSE
                     VAELASMTMELLTMDYWDEFYSNPEDLKKAKRDQLEGTLSFLPWCMTVDALQHWVYLH
                     PEHSVKERYDAFVDINKRFSAGVDWTGLENYRKILWTQQLHIFEVPFYYIEYGMAQLG
                     ALSIYMNYRQDKQKALQQYQDFLNLGYTKSVKDIYKTAGISFDFSEDRIYTLVNFVRE
                     ELEKLED"
     gene            complement(8887..9291)
                     /locus_tag="PKM80_01330"
     CDS             complement(8887..9291)
                     /locus_tag="PKM80_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HNV92580.1"
                     /translation="MDIKDIFSQIRSMVNNAMGGGLSFGQPSKLGDLYVIPVARVIYG
                     MGGGGDQPSSEQETEKEEKTETDKAENKTKVKVAFSGGGGVGTFSKPVGLFSIKEDRI
                     KFHPVIDLMELVALGSFMTIFMIIMKKLRFIK"
     gene            complement(9430..11277)
                     /locus_tag="PKM80_01335"
     CDS             complement(9430..11277)
                     /locus_tag="PKM80_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNV92581.1"
                     /translation="MKNFKMLICSFLLVCGGSLCGSITRYYTFDYSTAEYNPINGSQL
                     TFPDMNNYISSGIEIGFPFPYCGEMYEYVKISTNGFINPGATWNDDSPNNQLEYGIYP
                     IIAPLWDDLSLGEGNLQYATIGSAPNREFWVQYSHARWPYNQFNCYVNFQVVLSENGK
                     IEFHYGNTNGTPVNGSASIGINMPNAGAGNFFSITPGNPPLVSTTQENYSINEIIPSG
                     TVYTFNPLPPVLYDLSLEIELDLIYMNGIYHNWQESFVLPVTVKNEGTETVDSYDVVL
                     FCQQMELCRLNGISLAPAESQLYELVATIDSSGWQYLTAVAELPEDEYPEDNIDGYTC
                     IIRPNPATGIIVGAGNELQRIPIDVYWKYSLYETIYYADEIGDSGTIYGISFYMNSTF
                     CHFLTVKVWIGETNLTNLSEGWIAAGELTPVYYSDINIPADSIEMYLPFSVPYQYHQG
                     NLCVMVYHTHSYLLTCSDPFLAQTVPEMRALKRWTDAYDPDPYNPPIYNDLYGKMPKT
                     AFYLISANDIDDEYLPIVKLLSCYPNPFSETVTLSLNLAKREQICLEIYNCKGQKVTT
                     LYNGNIEKGETKISWNGLDSNNRKVANGLYIGQLRTKEKCEKVKLIVLK"
     gene            complement(11827..>12066)
                     /locus_tag="PKM80_01340"
     CDS             complement(11827..>12066)
                     /locus_tag="PKM80_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HNV92582.1"
                     /translation="EEKRRDIVKSIKKISEDTRVAIRNIRRDSNELVKKQKKDSEISE
                     DEEKKQLKELQDITDDWIKKIDEAEKAKEKEIMEV"
BASE COUNT         3641 a         2400 c         2120 g         3905 t
ORIGIN      
        1 catatcttca gttctgcccc gagtagcgaa aagtgactga tgtccatccc tgaaagttaa
       61 atcctgaact ttaacaggat ttgcagcttt cgggcggtct ggttcataat gcatagtagt
      121 tttacttacg caactatgtt tatccattta tatctccttt tatctgcttc ttttaattac
      181 tcttcgttga gttaaatcac ggtattgcat agttaactgt ctgccatagg cagtataagg
      241 ctggctggga attcccctta ccggttcctt gtattgttca tatgtctctt cactttgaag
      301 aagagctaaa acagctccaa tggcagcaat ttctttttta tctttcataa tcttgtttcc
      361 ttgttttggt tagagaggaa tgttgccatg ttttttagga ggcaaacttt cactcttagt
      421 gcaaagtatt tccaaagcat ctattaagcg tttccgagtt tctgaaggta cgataacagc
      481 atccacataa ccacgcgaag cagcaacata aggattattg aactgctctt cataaatctt
      541 tatcatttcc tgccttttgg cttcttgatc ttcagcagaa gcgatttctt tacggaaaat
      601 aatatttgct gcaccttgag cacccataac agcaatttca gcagtgggcc aagcaaaaac
      661 catatctgcc cctaaatgtc tgctactcat agctatatag cttcccccat agtcctttct
      721 ggtaaccact gtcaatttgg gaaccgtagc ttcggaataa caccataaaa gtttggctcc
      781 gtgacgaata atgccattcc attcttgatg cgttcccgga aggtaacctg gcacatccac
      841 aaaagtgatt agcggaatat tgaaagcatc acaaaaacgc acaaaacgcg ttgctttatc
      901 ggaagcatca atatccaaac aaccggctaa gaaagctggc tgactggcta tgatgccaat
      961 tgtccttcca tttaaatgac taaaaccaat aatcacattt tgggcataat aatagtgggg
     1021 ctcaaaaaag attccatcat caaccacgct atgaatcaca tcccgcatat cataactcat
     1081 tttaggatca tcaggaataa tggaatccag ctctggacac aaacgcccgg gatcatcatt
     1141 tgtttttatc cggggtggtt cttccatatt attagaaggc aaataggaca gcagcacttt
     1201 tatctgttca atggcatcgg catcactttc acatgcaaaa tgggcatttc cacttttgct
     1261 attatgagcc attgcacccc ccaaatcttc ctgattggta tcttcgccag ttacggtacg
     1321 aattacatca ggaccggtaa taaacataaa gctggtattc ttcaccataa acacaaagtc
     1381 cgtcattgca ggagaataaa cagctccacc tgcacagggt cccataattg ctgttatttg
     1441 aggaattaca ccactggcac gcgaattacg gaaaaagata tctccataac cttttagagc
     1501 atcaactcct tcttgaatac gggctccacc tgaatcttga ataccaacta aggggactcc
     1561 actttttaaa gccatatcca taactttaca aattttggca gcatgcattt ctcccaagga
     1621 acccccacga gaagtaaaat cctgactgaa ggcaaaaacc ggacgaccat ctactaaacc
     1681 atgaccagtt ataacaccat cggaagggat ttctattttt tccattccga aattatcgca
     1741 acggtgcgag acgaacatat ccagttcccg aaaagtgcct tcatcaaata ggaggtctat
     1801 acgttcacgg gcagttagtt ttcccccttg cttttgtttt gcaacagctt tatcaccacc
     1861 catttggagt attttcttct ctttttccag taagccctgg atcgcatctt ttgtagattg
     1921 cattagattt cctcactgtg tatataataa ttaatcgaaa ataaaaaaca aattgctttt
     1981 actacccttg ttaataggga tagagtataa atttacattg tgttctcctt ctagtgcatt
     2041 atttttttag tggatacagt gctttgttga tggcaactat aatcctatag tatttatagc
     2101 tattttgcct ttttctcata gagctttttt ttgtcaaatc aaatttatgt aaaacctgta
     2161 agttgatatc tatgcatact ctacccaaat atctataaag taatctaaaa tgaatgggaa
     2221 gcttcataac ttggctgcaa aaagagcgat gaggacatcg atcttccgac attatagcag
     2281 cagaatatgg tttttatcgt ttttttcatt tgacagaatt gactatatta tcctatatga
     2341 attaattaat attaaaagga ctgaataaat cgatggctcg tctttttgca aaggactatc
     2401 acaacaaatc taccagctgg tatttcaatc aggcagttat tgttctggaa aaagatgatt
     2461 tatggttttg gaaaacccgt aatgaagagg gaaactatca aattcgcttt tttccggttc
     2521 cccagaaaag agaatatttt actgaatatg tagatattgt tttcaatccg gaaaccgagc
     2581 tgataatttc tcataattgt tctcagtgta atactgatga atcctgtcgt cattatctat
     2641 ctgttttacg ctatgcttat ctttatttaa gcacgcgtat ttttgaagaa gacacagttg
     2701 agacctgtga tggtaatgcc ttagcaggag atgaattatg gctaaatctt gctcgcaatg
     2761 caaaactcta tgtggaagga atttacaatc ccgaaacgga taaaatccgg ttttaccata
     2821 atgaacttag tcccttggac ccagttttaa ttggcaaatt atatgctcaa gaagagataa
     2881 cagatatttc tgaaaggcaa gttgaatatt atctttccaa tttaagtgcc ttttgggatg
     2941 aagaactgca tctttttagc tttttaaaca aaaaccgagc ggcctatagt cgtaaaagca
     3001 aattttggtc tatatataaa aaagattttg ctgcagcact tttaataatg caaaaatgtc
     3061 gcactcgttt tatcgttaaa gaaaccggag aggagcttat ttttcaggaa cagacctatc
     3121 cgcttgcttt aaggattgaa cctgctggaa ctaataacta ttctttgaga gcagtagttg
     3181 tagatgaact ttcagtatgg tttaccggaa atcctagctg gctatttttt cgtaatcgga
     3241 tttataaaat ttatctacct tttcgcaaag aagtggtcga taatattttt agcagcggag
     3301 aaatacttag tactcgggat ttagtttatt atcgttgtat tgttcatcgc gaacttcacg
     3361 agcataatat ataccttgat tttgaggata gtatagttct tccagaaatt gttgatgaga
     3421 cacctctaaa gcgattacat atcaaaaaat tgggcgataa agttctggtt ggaggttctt
     3481 tagctttttc cggcgaacga gaaatccctc tttccgtgtt gcgttttcaa aaacctctaa
     3541 ttcattccaa atatgtttct cctgcaggtg aaggcaatgc ttggtttcat ctttcacctg
     3601 aactttatga aaaaacacag gaattgcttg aaaaacttcc tgaaccggaa ttaaaccgtc
     3661 tggaagaata tgctgaactc gtttttagtg gtgaagaaag aattgaacag ctacgaactg
     3721 ctatttttaa tcttagtgac gaagattggg atattcaaat tgatcctgaa ctgcaaaatt
     3781 acttcatttc taaaatccct ctgcaggtgg aaattattgc ccgtacagat gaagatattg
     3841 attggtttac ttatgaagtg cgttatcgct atcgagattt aaatttcacg cacgaagagt
     3901 tgaaacgttt tttccgcagt aaagaggaat ttttgcatac tgcagaaggc agaattgtgt
     3961 ttatcagcaa tccccaggtt ttttatgaag tggaagaact catttctcac agtgaacaga
     4021 aagcggataa agtatatcgt tctagattga tgaatctgcc atattattta agactgcgcg
     4081 aagaaaatcc cgcttttcga atgttgggag atgaatggct ggaaggactt tatgatgcct
     4141 tactaaatag acatttaaaa acttttgaat ccttaccctt atatttacag acagtattgc
     4201 gtggttatca aaaagcaggt gttgcctgga ttaagatgtt ggcacattat cacttaaatg
     4261 gtattcttgc cgatgaaatg ggtttaggaa aaaccattca agccctctcg gctattttat
     4321 ctacaactgc cggaaaggtt tccctggtta tttgtcccaa aacattactt tacaactggg
     4381 ctgctgaaat cgataaattc catactaata tcccttatgc cattgtggat ggtaataaag
     4441 aaacccgcat tgaaattctc tccaatccta atgttcgact ctttattatg agttattcta
     4501 tggttttggg agatattgct tatctgaaga atatggagtt tgaatggatt gttctggatg
     4561 aggcacagaa catcaaaaat gtttccgcac aacgcacttc tgctattaaa aaactgaaca
     4621 gcaaacaccg cttggctcta acaggaaccc ctatcgaaaa taatctaacc gagctttggt
     4681 ctatctttga ttttctgaat ccaggttatt taggtaccct gaataaattt aagcagaatt
     4741 atcttcctgc tgaaggagaa ataaaagccc ggctttcttt acaaaaaatg gttgctcctt
     4801 tcttactgcg tagagtgaaa aaagaagttt tattggaact tcccgataaa caggaacaaa
     4861 tatcttggtg taaactaaat actctacaag aaaaactata cctgcaaatc ttggatatgg
     4921 tgcataaaaa acttctgccc gaaggccaag aaatgcaaag ctatattcat atcttagcag
     4981 cacttacaaa gcttcgccaa gtttgcaatc acccccatct tgccaatagt gacatattag
     5041 cagaactgga agcatcttcc aaattggaac aacttttgga attggtaacc gaagcgacaa
     5101 atgcaggaca taaggtcttg gttttcagtc agtttgtaca aatgctatct attattcgca
     5161 aggtttttga tgcccattcc ttatcctata gttatttgga tggtcaaacg aaagatagag
     5221 ttaccccaat taaacgcttt gaaactaatc ctgatatcaa actctttctt atttctttga
     5281 aagcaggagg taccggctta aatttaaccg ctgctgatac agttattttg tatgatccct
     5341 ggtggaatcc tatggtggaa aatcaagcaa ttgatagaac tcatagaatt ggccaaaccc
     5401 aaaaagttca ggtttttcgt ttaatcacca aaggcaccgt agaagaaaaa atattacagc
     5461 tacaggaaag taaacgcgaa ctctttgata ccgttattga gggcggacag aatatgataa
     5521 aggctatgac aaaagaagat ataaaatcgc ttttcagtta ctcaggatga agaaggtaga
     5581 attacaggtt cagtgaatcc tctttgtgga atggaagtag agaaggttaa agataagggg
     5641 aaaaagtgga aaagttagac ccaagacgaa catcgtataa tagatagata ataaaagtta
     5701 taagttattg tgtagccgga gctccgcatt ttgtgtagtc agagctcgct gaagctctgc
     5761 attctttcaa ttattatttt ttgcggagga acggcgtcct ccgtctacac atcaaaatcc
     5821 tccgaatcct caaacccttg tttctgaaac tcaaatctgt acgaaatatc ttcggtgttt
     5881 tcggtggctt ataaacaaat cctcttaatc ctaaaatcct taaatccttg tttctaaaac
     5941 tcaaatctgc gtaatctgcg aaatctgcgt gagatagaaa aagcggagga acggcgtcct
     6001 ccgtctacac atcaaaatcc tccgaatcct caaacccttg tttctgaaac tcaaatctgt
     6061 acgaaatatc ttcggtgttt tcggtggctt ataaacaaat cctcttaatc ctaaaatcct
     6121 taaatccttg tttctaaaac tcaaatctgc gtaatctgcg aaatctgcgt gagaaaaaat
     6181 tagaacatag cgctaagcaa ctcatccaga tttacttcgc cgaagtagct ttgatagtcc
     6241 atttgttgta gcacttttgt cacagtatca ggtttatgtt caattccgcg gagagcgttt
     6301 tccagttcgc tgatttcgcg gttggtaaag aaatcaccgt aaatactcac ttcttctata
     6361 attcccttat tcacataaag gaaaaattca atcacaccag cttgagtctt aatagaatgg
     6421 gataaattat agcgaggaga ttttccgaaa ttccattgcc aagtatcgta tttactattc
     6481 atcagggctt ggatttcttc tttatctttc aaggatagaa agtaatcctg aatttcagga
     6541 tacataattt tcactttagc tctaattaaa gttacaaaat cttgcaaaga aagcggatag
     6601 ggaagatgat cagaaacatt ggtaactcgt gcctgcacag attttaccgc tttatcgtta
     6661 aatttttctt ctctaggatt tagagcagcg ctaagattgg taatatttga actgaaaagg
     6721 atggtgccat gttgcaaggt ttttccaaag gcaacagttt tcgcgttacc ggaaaactta
     6781 cagccattaa tagtaaggtc atttctgcct tccaaaatag caggaacacc caaatcatgt
     6841 aatatagcta aaattggttt agtatagcgt tcaaaactac gattcatatc tccattttct
     6901 ttcaccataa atgaataatt caggttacca gaatcatgaa atacagttcc gccaccggtt
     6961 aaccgacgca ctactggaat attatgtttc aggacccatt cataattaat ttccgccaaa
     7021 gtgttttgat gttttcctac aatgatgctg gaatcgttaa tatagagcag aaatacatcc
     7081 tcagaaaaat cccttaggat atattcttcg caggcaatat taaaaggagc atagttgctc
     7141 gggctgaaga tagagagcat tttaatcctc caatttttcc agttcttctc ttacaaaatt
     7201 caccagagta taaattctat cttcagaaaa gtcaaaagaa atccctgcag ttttatagat
     7261 atcctttacg gattttgtat atccgagatt cagaaaatct tgatattgct gtagtgcttt
     7321 ttgcttatcc tggcgataat tcatatagat agaaagcgct cccaattgtg ccattccata
     7381 ttctatataa tagaaaggca cttcaaaaat gtggagctgt tgcgtccaca gaattttacg
     7441 ataattttcc agaccagtcc aatctactcc cgctgaaaag cgtttattaa tatcaacaaa
     7501 ggcatcatat ctttccttaa cgctatgttc tggatgtaaa taaacccaat gttgcaaagc
     7561 gtccacagtc atacaccaag gaagaaagct taatgtccct tccaattgat cgcgttttgc
     7621 tttttttaga tcttccggat tggagtaaaa ttcgtcccaa taatccattg ttaaaagttc
     7681 catcgtcatt gaagccagtt ctgccacttc cgaaggagtt tccaaatact gagcaatttt
     7741 gatattcgcc atggcagcgc tatgcattgc atgtcccgat tcgtgcagta aagtaacaat
     7801 atcattatga attttcacat agttcataaa aatgaaagaa cttgccagat tgttaatcga
     7861 gctgttatag cccccgggag ctttgccttt acggttttcc aaatccagta gacccgtatt
     7921 tttcatcttt tccaattgca ttgcatattc aggatttact tgatagagca ctttcagcga
     7981 cttattgata aattcttccg ttgtttcaaa gggttttaaa atgcgcccat ccaagtccac
     8041 agcagtatcc caaggtctta atgtatccag ttttaatgcc tcttttcttt ttttgttttg
     8101 ttccgcaata aaaggcatca ccactttctc cactgactta tgaaaagcaa aaagttcctc
     8161 tggagtatat gaaaaccgcc ccatctcctg atgcttgaaa tcacgataat tgctaaaatc
     8221 aacatttaca gcaatttgat gacgcaaagc aatcagatca gaatagagct tattcagttc
     8281 ttcctgtttt tcagcaaaca tattcattct caggcgccat gcgttttccc gtttgtttct
     8341 atcaggatct ttcagataaa cggaaagttg tgccggggtc ttttcttcgc cttcaaaaat
     8401 agcactcatt ttgctgacaa tcccggcata tttatttgcc aattccgatt cctgaatcat
     8461 caaggggata ttttcctcgc ggaaaagctt tatatcatta ctaaatattt gattcagcaa
     8521 actgtatttt ctggattcca attctgtgcg gacagggctt tcataaaaac gctttttaat
     8581 tttaaattga tatgcctctg cgggagcata aatattggca aaataatcgt tatatgcttg
     8641 ttctttagtt tcgtcatcgg catttacagt catttttata taacgccagg atagttcatc
     8701 tgccatacat ttcagcaggt cagaatattt taataacata gtttccaaat tatcaatagt
     8761 gctaacatca tgttgcacaa gttcttctaa gaatgcttga acttgttccc aagaagagac
     8821 atcaaaatcc acaggcagat tttcgtaggg catctgcttt aacttttcat cagttatgaa
     8881 catttcttac tttataaacc tcaacttctt cattattatc ataaatatag tcataaaact
     8941 tcctaaagcc actaattcca taagatcaat tacaggatgg aatttaattc tatcttcttt
     9001 aattgaaaat agacctactg gcttactaaa agttcctacg cctcctccac cactgaacgc
     9061 aactttcacc tttgtcttat tttctgcttt atcggtttcg gttttctctt ctttttcagt
     9121 ttcttgttca gaagagggtt ggtcgcctcc accacccata ccataaataa ctctggcaac
     9181 cggaatcaca taaagatctc ccaatttaga aggttgccca aaagataaac caccgcccat
     9241 agcgttattt accatagagc gaatttggga aaagatgtcc ttaatatcca tagccaactc
     9301 cttattcata tctctatttt atatattatg ttttttttgt caagcgacaa ttgcactttt
     9361 cgctaaactg gataccagaa gtctgttaat tatctttgcc taaaactgat tataagttag
     9421 cttaaggttc tatttcaaca ctatcaattt taccttttcg cacttctctt ttgttctcag
     9481 ctgaccaata tacaagccat tggcaacttt cctgttattg ctgtctaaac cattccaaga
     9541 tattttagtt tcgccttttt ctatattgcc gttatatagg gttgtaactt tttgcccttt
     9601 gcaattgtat atttctaagc agatctgttc tcttttagcc aagtttaagc ttagggttac
     9661 agtttctgaa aagggattag gatagcagga gagtaattta acaatgggca gatattcatc
     9721 atcaatatcg ttagctgaaa ttaaataaaa agccgttttt ggcatcttac catataagtc
     9781 attgtaaatg ggaggattat aaggatcagg atcgtaggca tcagtccagc gttttagagc
     9841 tctcatttct ggaaccgttt gagccaaaaa aggatccgaa caggtaagta gataagaatg
     9901 agtatgatat accattacgc acaaattgcc ctgatgatac tgataaggga ctgaaaatgg
     9961 cagatacatt tcaatgctat ccgcaggtat gttaatatcg gaataatata ctggcgtcaa
    10021 ttctcctgca gcaatccagc cctcgcttaa atttgttaaa ttggtttccc ctatccaaac
    10081 ctttactgtc agaaagtgac aaaaagtgga attcatataa aaactaatgc cataaatagt
    10141 tccgctatca ccaatttcgt cagcatagta aattgtttcg tagagtgaat atttccagta
    10201 aacatctatg ggtattcttt gaagttcatt tcccgcacct acaatgatac ctgtagcagg
    10261 attagggcgg ataatacagg tataaccgtc aatattatcc tccggatatt catcttcggg
    10321 aagttctgct accgctgtta aatattgcca tccactggaa tcaatagttg ctactaattc
    10381 atataattgg ctttctgcag gtgccagaga aataccattt aaacgacaaa gctccatttg
    10441 ctggcaaaaa agaaccacat catagctatc aactgtttca gttccttcat tttttactgt
    10501 taccggaaga acaaaacttt cttgccaatt gtgatatatc ccattcatat aaattaaatc
    10561 taattctatc tcaagcgaaa gatcataaag cacaggaggt aatggattga aagtgtaaac
    10621 tgttccggaa ggaataattt cgttgatact ataattttct tgcgtagtag aaaccaaagg
    10681 aggatttcca ggagtgatac taaaaaaatt gcccgctcca gcattaggca tattaattcc
    10741 gatggacgca gaaccattta caggagttcc attcgtgttc ccgtaatgga attcaatttt
    10801 accattttcg gaaagcacta cttgaaagtt tacataacag ttaaattgat tatagggcca
    10861 gcgagcatgg gagtattgaa cccaaaattc acggttggga gcagagccaa ttgttgcata
    10921 ttgtaaattg ccttccccca aactaagatc atcccaaagg ggagcgatta taggataaat
    10981 gccatattct aactgattat tagggctgtc atcattccaa gtggcacccg gattgataaa
    11041 accattagta gaaattttaa catattcata catctctccg caatagggaa aaggaaaacc
    11101 gatttctatg ccagaactga tataattgtt catatctgga aaagtaagtt gacttccgtt
    11161 tataggattg tattcagcag tagaatagtc aaaagtataa tagcgggtaa tggagccaca
    11221 caagctccct ccgcagacaa gcagaaatga acaaattagc attttgaagt ttttcatcgt
    11281 ccatctcctt ttggaaagga accgaaacct aatattctca ttttccttta tattatttct
    11341 tcaaaactaa gtcaagcatt ttcttatttt actattttct tattgcttgc ctatatacat
    11401 ctcctcctcc tcccatcttc aactcctcct ccactcgtca ttaaggatga gtaaaggttg
    11461 ggtgaattag gagagaggat aaagggagag agaataacta gatgatagat gaagaactaa
    11521 gaatgtctat ggtttcgccg ggttaaaacc cgtcgctcaa atctgtcgtc ccgctgggac
    11581 ttttttatcc gttctttttt ttcgccgggt taaaacccgt cgttagtatg tatcgttcct
    11641 aacggaacta atctctttat gtaaatttgt ttcgagggct tatgctctat cgctatgatt
    11701 gtgtcggcct gaggggcttt tattggctat ataattcaat cctgtaaatc cctttcatct
    11761 tgttcatccc atacctattc tttttttaat ctgcgtaatc tgcgtaatct gcgtgagata
    11821 aaccctctac acttccatta tctctttttc ttttgctttt tctgcttcgt ctattttttt
    11881 aatccagtcg tcggttatat cttggagttc ttttagttgt tttttttctt catcttcgct
    11941 gatttcgcta tcctttttct gttttttaac cagctcatta gaatcgcggc gaatatttcg
    12001 aatagcaact ctggtatctt cggatatttt tttgatgctt ttcacaatat ctctgcgttt
    12061 ttcttc
//
LOCUS       DAORHB010000022        11837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_4562_length_11837_cov_4.245629, whole genome shotgun sequence.
ACCESSION   DAORHB010000022 DAORHB010000000
VERSION     DAORHB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4562_length_11837_cov_4.245629"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..2459)
                     /locus_tag="PKM80_01345"
     CDS             complement(291..2459)
                     /locus_tag="PKM80_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNV92583.1"
                     /translation="MIGGGGGGGHSGRGAHFEDELKNKIYDRRLFSNVIHYIKPYLKW
                     VILSFLFLMLISGAEVIIPLIQRSAIDDYIVSDKTLAIFTQEDDYHEFINRYQKLGFK
                     EYKYGDNHFLVISSKDRNKMDRADLEKLKAKGILKEDTVFLVSAKDKNISILKQYLPE
                     NINPEQPNLTGWFKISSETIAIPKEQLKELPKAKRLEVRSDASKQLLILALIFLAIIT
                     LRFVASYFQTVFTAYFSQTAMADLRHDVFAHLQKMPTKFFDTNPVGRLVTRVTNDIAA
                     IDEMLASGVITIIQDVVLIITIVILMLALNWHLALVSFSILPLVIWVIVIFRRKTRVV
                     YREVRKHLAVLNATLAEHIEGQKIIQLFNQYFHKRQEFADINQKYYLASIKQIRLFAF
                     FRPIIHVSSQIAVALIIWYGGGKILQNVITIGLLMAFTQYIQKLFEPINDFSEKFNFL
                     QSALASAERIFNLLALSPDDYREEKHQNIKLEGEIEFENVWLAYNENEWVLKDISFKV
                     APGEKIALVGHTGSGKTSIVNLILGMYPYQKGRILIDGKDIHNYSLADLRRNVGIVQQ
                     DVFLFSGNIKDNIALNNDELSDEEIIQVAKYVNADKFISKLPNGYNEPVMERGATLST
                     GQRQLIAFARVLAYNPSIFILDEATSNIDTETELLIQDALNKIIKDRTSIIIAHRLST
                     IQNVDRILVLHKGEIVEEGSHFELLDKQGLYYDLYRLQYT"
     gene            complement(2459..4210)
                     /locus_tag="PKM80_01350"
     CDS             complement(2459..4210)
                     /locus_tag="PKM80_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92584.1"
                     /translation="MRTFDAVLPYLKKSYTRILGGLVMLILVDLVQLAVPKVMQYAID
                     HIQERTIDYRGLAWIGLIIFGCAIAVMVLRYFWRILIIGNSHLIEKSLRQDYYDHLLT
                     LSQNFFNKSKTGDLMAYATNDLNAVRMLFGMGLISAMDIVLMTIASFSFMVSINAKLT
                     LLAIIPMPIISLTVLYFGKKVHTRFKAVQESFATLSGKVQETISGIRIVKAFCQEKNE
                     LKKIDKVSHDFVDQNLALAKIAGVFHPFMGFIISISMIITLYFGGKAAIRGEISVGGF
                     IAFFQYLGMLVWPMIAIGHIVDMYQRGTASLKRLNDIFEVNPEIDDAFADERITHLKG
                     KIQFQNLSFRYADHLPFIFNDITAGIEAGKTMAIVGPTGCGKTTLIELLVRIYNPPRN
                     SIYIDDYELFTIPLNVLRRDIVLVPQDIFLFSETIADNIRLGNPEASDEEVFEAARTA
                     QVYDEIMEFEHKFDTIVGERGVTLSGGQKQRVAIARALLTNPNILILDDALSAVDTKT
                     ERRILNRLIEIRKGKTTFIIAHRISALQHSDLIIVINNGIIAEQGTHQELLAKGGLYK
                     DLYEKQRIRAKIEGEEE"
     gene            5008..5481
                     /locus_tag="PKM80_01355"
     CDS             5008..5481
                     /locus_tag="PKM80_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV92585.1"
                     /translation="MNKLFMPELVKIIEKFENKNACLHYMADLLSESGCLSFPDRFLA
                     AVKGREEIMSTGIGRGIAIPHARDLTVSSLKIAVCMIKQGLDFTSVDNLPVNLVFMIA
                     VPQSSNQDYMKILRSLSGFLRRDENRESLLQATDERDLYEKVQVLEEELRPQLGV"
     gene            5471..6400
                     /locus_tag="PKM80_01360"
     CDS             5471..6400
                     /locus_tag="PKM80_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNV92586.1"
                     /translation="MAFSFLIILPLFSQHQDAGTTGFSTLKIYYSARANAMGQAMLGR
                     AMNFEGMQFNPATLIKAKNKAVYTTFADHFVGSGGGSINYMVPKNIYTSYGFYLNYWN
                     SGSIDRTDIGANGEFIELNDTFGAQDITAGFSVAKFVSPALDAGGSVKLVLDQIDGKS
                     ATAALIDLGILHHTPNEKIKVGLTIRNLGFQLSHYTNKDYSEGIPITFGAGIGIDAAK
                     NTLLNIDLNKANGENFIGKFGIEQSVHPSLVLRAGFKTNGGDYSIGGDLGWSSGISLG
                     LGWTWKKYAIDYAIASYGDLGFTNQVSIRYNFD"
     gene            6437..8530
                     /gene="glyS"
                     /locus_tag="PKM80_01365"
     CDS             6437..8530
                     /gene="glyS"
                     /locus_tag="PKM80_01365"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNV92587.1"
                     /translation="MKQNQFLLELGVEELPEKQITIACNSVKLSFANFLKENKLNCSG
                     YKVSGTPRRIFLQAIDLDSHQKDEEIVKTGPAFRLAYDEAGNLTNAGKGFLKRNQVEE
                     SAIFIQETDKGKFLAVKYIKTGISTGELLQKWIPDMILQIPFDKKMLWNKPGFAFSRP
                     LRWLLILLNNNPLTIDFFGIPCGNTTYGNRYLGLDRCITINKAEEYIPKLLEHKVVAE
                     IDERRKEIVDQMANIFPKGDYRVIKDLPLVETVVNLVEFPTAVCGEFEHKYLSLPDKI
                     IISTISQAQKYFAVHDKIGKLSNKFVFISNGDPAAWDLIKQGNEKVVNARLADALWYW
                     NEDTKHPLDYWTKHLDMVVFQAKLGTMADKTKRIICLTELIAKELNLPSEQREKVLRC
                     AELCKADLVTNMLGEKEFTKLQGYIGKQYAIVSGEDEEVAEGIYEHYMPRGSADKLPS
                     TLCGALVAIADKMDTVAGIIGIGMLPTGSGDPFALRRAANGIVQIISHRKWDFNIFAL
                     ADEALELISKQTELDKNAYSNLHSFIEQRITGLLKTKGIAYDVIDSVMHIDKSHIYDL
                     ENRAKALNDLKSKPNFIHLVIGFKRVANIIAETTEFVPFDKNKLVEPEEINLYISLQK
                     LHNDIDSALQKKDYPLALQYLISFGKVIDAFFDAVLVNCDDEVLHNNRHSLLREVKNE
                     FLRVADLSLIVLETE"
     gene            8543..9547
                     /gene="pta"
                     /locus_tag="PKM80_01370"
     CDS             8543..9547
                     /gene="pta"
                     /locus_tag="PKM80_01370"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNV92588.1"
                     /translation="MFIIDEVKKKAKKTKGSVVLPESSDPRVLIAAAQIVKEDLAKII
                     LLGKEEKISEDAKALNLDLNGVTIINPETSEYLSAFADAFYLRRKHKGVNPVQALETV
                     KDPLYFGASLVKQGKVSGMVAGAANTTADVLRAALQVIGVMPGLKTVSSTFIMPVPDF
                     RGEPKVFLFADCAVVPNPTAEQLADIAICTALTRKAIIGDEPKVALLSFSTLGSANDE
                     LVDKVISAKAILDERKVDFEYDGELQLDAALIPEVAIRKAPQSPIAGKANTLIFPDLR
                     AGNIGYKLVQYIGKVKAIGPIIQGLAAPASDLSRGCSYEDIVNTVAYVLLLAAQQKYK
                     "
     gene            9572..10780
                     /locus_tag="PKM80_01375"
     CDS             9572..10780
                     /locus_tag="PKM80_01375"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV92589.1"
                     /translation="MATKLSNRCRLIKPSPTLSLSAKAKEMKDAGIDVISFSVGEPDF
                     PTPDYIKNAAHKAIDANYTRYTNNAGILELRQAICDKLLRDNDLKYSPKEILVSPGAK
                     ASIVNALTAVCDVKDQVLMGAPYWVSYPYQVALANAEPIYIPTKEEDGYKIRAAELEI
                     AIQENPCTKALILNSPGNPTGAVYTKEELAALAEICIKYDILVISDEIYERLVYDDVK
                     HISIASISEEMKQRTIVINGVSKAYAMTGWRLGYAAGPANIIATAGKVQEHSTSCVNS
                     ITQYACVTALNEEDDSIEKMRQEFSVRRDFLYNELKKIPHISCFKPQGAFYIMPGIKW
                     YLENNNQNIQTSDDFCAKLLDKYSVALVPGSSFGMEGTVRFSYANSMENIKEGISRFA
                     SFLQELNPGR"
     gene            10784..10999
                     /locus_tag="PKM80_01380"
     CDS             10784..10999
                     /locus_tag="PKM80_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92590.1"
                     /translation="MDDFNDKMNDRWKEKRKKAYNWPRLIIMILALIAILYIMNHLGN
                     TKNVVQTPSATVTDTLQADSVHKEITP"
     gene            10996..>11837
                     /locus_tag="PKM80_01385"
     CDS             10996..>11837
                     /locus_tag="PKM80_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNV92591.1"
                     /translation="MSLVIVGSIGLDSISTPAGEVCEALGGSAIYGSLAASHFTNTHI
                     VGVVGKDIPVEYLNLLQKSGINLDGLEVKDGKTFRWKGKYSTWNRVETLSTDLNVFAD
                     FSPQLPESCKCCRSLLLANIHPALQLQVLNQVSGYSHLACDTMNYWIELCPEEVEKVL
                     KRVDIVFMNEDEIRDYTKIADIYSAAYEILSLGPKWVIVKRGECGSVAVSKDDMYFAP
                     AYPVKKVKDPTGAGDSFAGAFMGYLADHDFVNHALIKEAILYGTVLAAMNVSDFSVQG
                     LVGKS"
BASE COUNT         3765 a         2065 c         2322 g         3685 t
ORIGIN      
        1 tgatcctcct tatagatcat aaatgatact ttcttctaat atactgccaa ggataaaata
       61 agattactcc gtaggatttg ggttattgta tacttatacc agagtttttt attagcaata
      121 tggataagca caatacggat aaaactctta tttaatcttg gcagaacact ctatcacata
      181 tataggtggt gaccaatttc gtcgataaat tatgaaagcc accattgtat attgatatca
      241 taattcgaat cccaaattct gtcaatcact ttttctatat cagcccaatt ttaggtatac
      301 tgtaatctat ataaatcgta atataagccc tgtttgtcaa ggagttcgaa atgcgaacct
      361 tcttcaacaa tttcaccttt atgcaacact aaaatgcgat caacattctg aattgtagaa
      421 agtcgatgag caattattat tgatgttcta tccttgataa ttttgtttaa cgcatcttga
      481 ataagaagtt cggtttcggt atctatattc gaagttgctt catccaaaat aaaaatggat
      541 ggattataag ccagcactct ggcaaaagcg ataagttgtc tttgccccgt agagagagtt
      601 gcacctcttt ccataaccgg ttcattatat ccattaggta atttactaat aaacttatca
      661 gcattaacat atttggctac ttggataatt tcttcatcgg aaagctcatc attattgaga
      721 gcaatattat cttttatatt tcccgagaag agaaaaacat cttgttgcac gataccaaca
      781 tttcttcgca ggtcagctaa agaatagtta tgtatatctt tcccatctat cagaattcta
      841 cctttttggt aggggtacat acccaagatc aaatttacga tagaggtttt gccactgcct
      901 gtatggccaa ctagagcgat tttttcgcca ggagcaactt tgaaagaaat atcctttagc
      961 acccattcgt tttcgttata ggcaagccaa acattttcga attcaatttc gccttccagc
     1021 ttaatattct gatgtttttc ttcccgataa tcatctggcg aaagagcaag taaattgaat
     1081 attctttctg cacttgccaa agcactctgc aagaagttaa atttctccga aaaatcgttt
     1141 atgggctcaa agagtttctg gatatattga gtgaaagcca tcagtaaacc gatagtgatt
     1201 acattttgta atattttccc tcctccatac caaataatca atgctacagc aatttgagat
     1261 gaaacatgga tgataggacg gaaaaaggca aacagacgga tctgttttat cgaggccaga
     1321 tagtattttt gatttatgtc cgcaaattct tgacgcttat gaaaatattg attgaagagc
     1381 tggataattt tttgcccttc gatatgctct gccaatgtag catttagaac agcaaggtgt
     1441 tttcgtactt cgcgatagac cactctggtc tttctacgga agataactat tacccaaatt
     1501 actagtggca agatactaaa gcttactaaa gccaaatgcc agtttaaagc cagcattaat
     1561 atcacaatcg taataattaa aactacatct tgaattatag taataactcc ggaagctaac
     1621 atttcatcaa tagcagcaat atcgtttgta actcgagtaa ccaaacgtcc cacaggattg
     1681 gtatcaaaga attttgtagg cattttttgc agatgagcaa aaacatcatg tctcagatca
     1741 gccattgcag tttgcgaaaa atatgccgta aatacagttt gaaaatagct ggctacaaag
     1801 cgtaaagtta taatggccaa aaagataagt gctaatatca ataattgctt tgaggcatcg
     1861 gaacgaacct ccaggcgttt tgctttaggt aattctttaa gttgttcctt gggaatagca
     1921 atagtttcag agctaatttt aaaccatccg gtaagatttg gttgctcagg atttatattt
     1981 tccggcagat attgttttaa gatgcttatg tttttgtctt tagcgcttac taagaatacc
     2041 gtatcttctt ttaaaatacc ttttgctttc agcttttcca agtccgctcg gtccattttg
     2101 ttacgatctt tggaactgat aacgagaaag tgattatcac catatttata ttctttaaat
     2161 ccaagttttt ggtagcggtt tataaattcg tgataatcat cttcttgggt aaaaatagcc
     2221 agagttttat cggacacgat ataatcatca atagccgatc tttgaattaa gggaataata
     2281 acttctgcac cggaaattaa catcaggaac agaaaagaaa gaataaccca ttttaggtag
     2341 ggttttatat aatgaatgac attgctaaaa agccgtctat cgtatatttt attctttagc
     2401 tcatcttcaa aatgtgcccc tcgtccagaa tgacctccac cgccaccacc gccaatcatt
     2461 attcttcttc tccttcgatt tttgctctaa ttctttgttt ttcatagagg tctttgtaca
     2521 atccaccctt tgccagaagc tcttgatggg ttccctgttc ggcaattatt ccattattga
     2581 taacaattat tagatcagaa tgttggagcg cagaaatgcg atgggcaatt ataaaagttg
     2641 tttttccttt cctaatctct attaagcggt tcaagatcct acgctcggtt tttgtatcta
     2701 ctgccgaaag agcatcgtcc aaaattaaaa tattaggatt agtaagcaaa gctctggcaa
     2761 tcgctacgcg ttgtttttgt cctccagaaa gagtaactcc tctttctcca acaatagtat
     2821 caaatttgtg ctcaaattcc attatttcat catatacctg agcagttctt gctgcctcaa
     2881 aaacttcctc atctgaagct tcaggattgc ccaaacgaat gttatcggca attgtttcag
     2941 aaaataggaa aatatcttga gggacaagca caatatctct tcgtaaaaca tttaagggaa
     3001 ttgtaaaaag ctcataatca tctatataga tactattgcg tggggggtta tatatcctta
     3061 ctaatagttc aattaaagta gttttaccac aacctgttgg accaacaatt gccattgttt
     3121 ttccagcttc tatccccgct gtgatgtcat taaaaataaa gggtagatga tctgcatagc
     3181 ggaaagataa attttggaat tggattttac cttttagatg agttatacgc tcatctgcaa
     3241 aggcatcatc aatttcagga ttaacttcaa atatatcatt caagcgtttc agggaagcag
     3301 ttccgcgttg gtacatatct acaatatggc caatagcaat cataggccaa actagcattc
     3361 ctaaatattg gaagaaagca ataaaaccac caacgcttat ttcgccacga atggctgcct
     3421 tacctccaaa atagagagtg ataatcatac ttatactgat aataaaaccc ataaaaggat
     3481 gaaatacacc agctattttc gctagcgcta aattctgatc tacaaaatcg tgagatacct
     3541 tatcaatttt ctttaattcg tttttttcct gacagaatgc cttcactatt ctgatgccag
     3601 aaatggtttc ctgaactttt ccacttaatg tagcaaaact ttcctgcaca gctttaaacc
     3661 gagtatgcac ctttttccca aaatacaaaa cagttaagga tataataggc ataggaatta
     3721 ttgccagtaa tgttaattta gcatttatcg aaaccataaa tgaaaaagag gcaatggtca
     3781 ttagcacaat atccattgca gaaatcaatc ccattccaaa aagcatccgt accgcattta
     3841 aatcatttgt agcataagcc attaaatcac cggttttgct tttattaaaa aaattctgag
     3901 ataaggttaa caaatgatcg tagtaatcct gacgcaagct cttttctatg agatgcgaat
     3961 tgccgattat cagaatccgc caaaaatatc ttagtaccat cactgctatg gcacaaccga
     4021 atataattag cccaatccaa gcaagacctc tgtagtcaat tgtccgttcc tggatgtgat
     4081 ctatggcata ttgcatcact ttagggacag caagctgcac aagatcaacc aaaattaaca
     4141 ttaccaatcc acccaaaatc cgggtgtagc tcttcttcaa atagggaaga acagcatcaa
     4201 atgttcgcat tttgctccca taatcaatta tttggggatg aaagctaacc tgaagaacag
     4261 gtgctataga ctattaaaac ccaaagtcaa cctttagaaa tggataattt tgtcaaatgt
     4321 tttcagttga tattgcatat tcctatatgg taaaaaacca atgtactatc ataaaatagg
     4381 ggatttttat agtatcgaga ttttaacaaa tagattgaat cttgcttaga tagttgtggc
     4441 agaactaccg tgatgctatg gagggaaatg cccaagaaat accactgtat ctctaccaca
     4501 tataattaag caagaaatat gtatactgag tccttctgaa aaaagtggac caaaattaag
     4561 aagaaagaaa tgaacaaaaa gtttacctaa aaggctgcat attcaggata ggatgccttc
     4621 gaatatataa gttgctcata tctgggcatt cagcgtgaat tatttttgcg gaagaacggc
     4681 gttctccgac tacccagttg tcatcttctt ggtatactct ttttaaatct gagagtatta
     4741 ttttcggtgg tttcgatggc ctcggtggct aaatcctctc aatccttaaa tcctaaaaat
     4801 ccttgtttct aaaattaagc cagcgtgaat tatttttgcg gaggaacggt gtcctccggc
     4861 tacccatttg tcatcttttt tgtatactct tttcaagcca gtgtgaatta atttagataa
     4921 tttggtttca tcaagcgaga agaattttga ttgacgataa atgccttgta aaaaactatg
     4981 tgtcttacaa aaaattatgg tgagacaatg aacaagcttt ttatgcctga gctggtaaaa
     5041 attatagaaa aattcgagaa taaaaatgcc tgtttacatt atatggcaga cctgctatcg
     5101 gaaagcggtt gtttaagttt tccagatcgt tttttggctg cagtaaaagg tagagaagaa
     5161 ataatgagta ctggtattgg aagaggaata gcaattccgc atgcaagaga tctaactgtt
     5221 agtagtttaa aaattgctgt atgtatgata aagcaaggat tagattttac aagtgtggat
     5281 aatttacctg taaatttggt atttatgatt gctgtgcctc aaagttccaa tcaggattat
     5341 atgaagatat tacgctcgct ttcaggcttt ttgaggcggg atgaaaaccg tgaatcgtta
     5401 ttacaagcaa ccgatgaaag agatctatat gaaaaagttc aggttcttga agaagagctt
     5461 cgccctcagc ttggcgttta gctttcttat aatattaccg ttattttcac agcatcagga
     5521 tgcgggaacg accggattct ctactttgaa gatttattat tctgctcgag ctaatgctat
     5581 ggggcaggca atgctgggga gagcgatgaa ttttgaaggg atgcaattca atcccgctac
     5641 cctaataaaa gctaaaaaca aggctgtcta tactactttt gcagatcatt ttgtaggttc
     5701 tggaggcgga tctataaatt atatggttcc caagaatatc tatacttcct atggttttta
     5761 tctcaattac tggaattctg gttctatcga cagaacggat atcggagcaa atggcgagtt
     5821 tattgaatta aacgatactt ttggagcaca ggatattacc gctgggtttt ctgtggccaa
     5881 atttgtcagt cctgctttag atgcaggtgg aagcgtaaaa cttgttttag atcaaattga
     5941 tggtaaatct gctactgctg ccttaattga tcttggaatt ttacatcaca ctcccaatga
     6001 aaaaataaaa gtggggctta ctatccgcaa tttaggcttt caattgagcc attatactaa
     6061 taaagattat tctgaaggaa taccgattac ttttggtgct ggtattggaa ttgatgccgc
     6121 aaaaaatact ctgctcaata tagacctaaa taaggctaat ggagaaaact tcatcgggaa
     6181 atttggcata gaacaaagtg ttcatccttc tctggtgctg agagctggtt ttaaaacaaa
     6241 tggtggcgat tattctatag gcggcgattt gggttggagc tctggaatca gtttaggctt
     6301 gggatggact tggaaaaaat atgcgattga ttatgcaatt gcttcttatg gcgatttagg
     6361 atttacaaat caggttagta tacgctataa ttttgactaa ctatagacga ctatatatta
     6421 aggtatagga aaacttttga aacagaacca atttttactt gaattgggag ttgaagaact
     6481 tcctgaaaaa cagattacta ttgcttgtaa tagtgtaaag ctatcttttg ccaatttttt
     6541 aaaggagaat aaacttaatt gctcgggtta taaagtgagt ggaacaccga gacgaatttt
     6601 cttgcaggca atagacctgg atagccatca aaaagatgaa gaaattgtta aaacaggtcc
     6661 cgcttttcgc ctcgcatatg atgaggcagg aaatttaacg aatgccggaa aaggtttttt
     6721 gaaaagaaat caggtggaag aaagcgcaat atttattcag gaaacagata aaggtaagtt
     6781 tttagcggta aaatatatca aaaccgggat atccacagga gaacttttac agaagtggat
     6841 cccggatatg attttacaga tacctttcga taaaaaaatg ctgtggaata aacctggttt
     6901 tgctttttcg cgtcctttac gctggcttct aattctgctt aataataatc ctcttactat
     6961 agattttttt ggtatacctt gtgggaatac aacttatggt aatcgctatt taggtttgga
     7021 tagatgcatt acaataaata aagctgaaga atatatcccc aagctgctgg aacataaagt
     7081 tgtagctgaa attgatgaac gccgcaaaga aattgtagac caaatggcta atatctttcc
     7141 caaaggtgat tatcgggtta taaaagacct ccctttagtg gaaacggtag taaacctggt
     7201 agaatttcct acagcagttt gcggagaatt tgaacacaag tatctatcac tacccgataa
     7261 aattattatc agtacaataa gtcaggcaca aaaatacttt gctgtacacg ataaaattgg
     7321 caagctgagt aacaagtttg tctttatttc caatggtgat ccagcagcct gggatcttat
     7381 taaacaggga aatgagaaag ttgtaaatgc tcgtttagct gatgcattgt ggtattggaa
     7441 cgaagataca aaacacccac tggattattg gaccaaacac ttggatatgg ttgtctttca
     7501 agcaaaattg ggaacaatgg ctgataaaac aaagagaata atttgcctaa ctgagcttat
     7561 agctaaagaa cttaatctac cttcagaaca gagagaaaaa gtgcttcgct gtgcagaact
     7621 gtgcaaagcg gatttagtta ctaatatgct aggcgaaaaa gaatttacca aactccaggg
     7681 atatattggt aaacagtatg ctattgtttc cggtgaagat gaagaagtgg cagaagggat
     7741 atatgaacac tatatgcccc gtggttctgc tgataagcta ccttctactt tgtgtggagc
     7801 tctggttgct attgctgata aaatggatac tgtggctgga attataggga ttggtatgct
     7861 tcccactgga agtggagatc cttttgctct tcgacgtgca gccaatggta tagttcaaat
     7921 tatcagccac cgaaaatggg attttaatat ctttgcttta gctgatgaag cattggaact
     7981 aatcagtaag caaacggaac tggataaaaa tgcttatagc aatctacata gctttattga
     8041 acaaaggata accggtttgc tgaaaacaaa aggcattgct tacgatgtga ttgatagcgt
     8101 tatgcatatc gataaaagcc atatatatga tctggaaaac agagcgaagg ctttgaatga
     8161 tttaaaaagc aaaccaaatt ttattcattt ggtgatcggt tttaaaaggg tagccaatat
     8221 tattgcagag accactgaat ttgtcccctt tgataaaaac aaattggttg aaccggaaga
     8281 gattaatcta tatatatcgt tgcaaaaatt gcataatgat atagattctg ctttacaaaa
     8341 aaaggattat cctctggctt tgcaatatct gatctcgttt ggcaaagtga tagatgcctt
     8401 ttttgatgct gttttagtaa attgtgacga cgaagtttta cataataata gacactccct
     8461 gctaagggaa gtgaaaaatg agtttttaag agttgccgat ctatcgttga tagtattgga
     8521 aaccgaataa caggaagaag aaatgttcat aattgacgaa gtgaaaaaaa aagctaaaaa
     8581 aactaagggc agcgtagttt tacccgaatc atctgatcct cgggttttaa ttgctgccgc
     8641 tcaaatcgtg aaggaggatt tggcaaaaat aatactatta ggcaaggagg aaaagatatc
     8701 tgaggatgct aaagcattga atctggattt gaatggtgta acgataatta atcctgagac
     8761 ctcagaatac ctatctgctt ttgctgatgc cttttatctg aggcgcaaac ataaaggagt
     8821 aaatcctgtc caagcattgg agacggttaa agatcccctt tattttggcg cttctttagt
     8881 aaaacaggga aaggttagtg gaatggttgc tggagctgct aatacgaccg ctgatgtttt
     8941 aagggcggct ctacaggtga ttggtgtgat gcctggattg aaaactgttt ccagtacttt
     9001 tataatgccg gttcctgatt tcagaggtga acccaaggtc tttttgtttg ccgattgtgc
     9061 tgttgttcca aatcctactg ctgaacaact tgctgatata gctatatgta ctgctctaac
     9121 gcgaaaagca attattggag atgaaccaaa agttgccctg ctttcttttt ctaccttagg
     9181 cagcgcaaac gatgaattgg tggataaagt tatatctgcg aaagcaattt tagatgaaag
     9241 aaaggtagat tttgagtatg atggtgaatt acagcttgat gcagccctca ttccagaagt
     9301 ggcaattaga aaagctcctc aaagtcctat tgctggaaaa gctaataccc ttattttccc
     9361 ggacttgcga gctggaaaca ttggttataa actggttcaa tatataggaa aagtaaaagc
     9421 cataggacca atcattcaag gtctggctgc tcctgcaagt gatttgtctc gcgggtgctc
     9481 ttacgaagat attgtgaata cagtggctta tgttcttctt ttagctgctc aacaaaaata
     9541 taaataaaat aaaaaaatac tggaggcaaa gatggccaca aagctatcca accgctgccg
     9601 tttgattaag ccctcgccta cacttagttt atctgctaag gcgaaggaaa tgaaggatgc
     9661 tggaattgat gtaatcagtt tcagtgtcgg agagccggat tttcctactc cggattatat
     9721 taaaaatgct gctcataaag ctattgacgc taattatact cgttatacca ataatgctgg
     9781 tatcctggaa ttgagacagg caatttgtga taaacttcta cgagataatg atcttaaata
     9841 ttcccccaaa gaaatcctgg tttcgccagg ggctaaagca tcaattgtta atgctttaac
     9901 tgcagtatgt gatgtaaagg atcaagtgct aatgggagca ccttattggg taagttatcc
     9961 ctatcaagtt gccctagcca atgcagaacc aatatatatt cctaccaaag aagaagatgg
    10021 ctataaaatt cgagctgcag aactggaaat tgctatacag gaaaatcctt gcactaaagc
    10081 cctgattcta aattctcccg gaaatcctac tggtgctgtg tatacaaaag aagaacttgc
    10141 tgctcttgct gaaatatgta tcaaatacga tattctggtt atttcagatg aaatctatga
    10201 acgcctggtt tatgacgatg taaaacatat atccatagcc agtatcagtg aagaaatgaa
    10261 acaacggaca attgttataa acggagtttc taaggcttat gcgatgacag gttggcggct
    10321 gggttatgct gcaggacctg caaatattat tgctacggcg gggaaagttc aggaacatag
    10381 cacttcctgt gtaaattcta ttactcagta tgcttgtgta acagctttaa atgaagaaga
    10441 tgattccatc gaaaaaatgc gacaagaatt ttctgtccgc agagattttc tctataacga
    10501 actgaaaaag attcctcata tttcctgttt taaaccccag ggtgcttttt atattatgcc
    10561 aggcataaaa tggtatttgg aaaacaacaa tcagaatatt caaacttctg atgatttttg
    10621 tgctaaatta cttgacaaat atagtgttgc cctagtacct ggtagttctt ttgggatgga
    10681 aggaaccgta cggttctcct atgcaaacag tatggaaaac attaaggaag gaattagtcg
    10741 ctttgcttcc tttttacagg agctaaatcc tgggaggtaa aagatggatg attttaacga
    10801 taaaatgaat gatcgctgga aagaaaagcg caagaaagca tataattggc ctagactgat
    10861 tattatgata cttgctctga tagcaattct ttacattatg aatcatctgg gaaatactaa
    10921 aaatgtggtg caaactcctt cagcaactgt aacagacaca cttcaggcag attctgtgca
    10981 caaggagata acaccttgag tttagttatc gttggttcaa ttggtttaga tagcatatct
    11041 acacctgcag gagaagtttg tgaagctttg ggtggttctg ctatatatgg ttctttggcg
    11101 gcttcccatt ttaccaatac tcatattgtt ggtgtagtag gaaaagatat acctgttgaa
    11161 tatctaaacc tgctgcaaaa aagtggcatc aatttagatg gcttagaggt taaagatggt
    11221 aaaacttttc gctggaaagg gaaatattct acctggaata gagtggaaac cctttctacc
    11281 gatttaaatg tatttgccga tttttctcct cagctgcctg aaagctgtaa atgctgtcgt
    11341 agtttactgc ttgccaatat ccatccagca ttgcaattac aagtgctaaa tcaggtatcg
    11401 ggatattcgc atctggcttg tgacactatg aattattgga tagagctgtg tcctgaagaa
    11461 gtagaaaaag tgctgaaacg ggtagatata gtttttatga acgaggatga aattcgggat
    11521 tatacgaaaa ttgcggatat ctactctgct gcgtatgaaa ttctatcatt aggtcctaaa
    11581 tgggttattg ttaaacgcgg tgaatgtggt tcagtagctg tttctaagga tgatatgtat
    11641 tttgctccgg cttatcctgt aaaaaaagtt aaagacccaa ctggagcagg tgatagtttt
    11701 gctggtgctt ttatgggcta tctggctgat cacgattttg tgaatcatgc tttaattaaa
    11761 gaagcaatcc tttatggaac agttcttgct gcaatgaatg tatcagattt tagcgttcag
    11821 ggtttagtgg ggaaatc
//
LOCUS       DAORHB010000023        11701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_4664_length_11701_cov_6.503521, whole genome shotgun sequence.
ACCESSION   DAORHB010000023 DAORHB010000000
VERSION     DAORHB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11701
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4664_length_11701_cov_6.503521"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PKM80_01390"
     CDS             complement(<1..733)
                     /locus_tag="PKM80_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV92592.1"
                     /translation="MMWIVKLFHTIWQMILLWSFVCRSYFIRLFAKNPIKRRWLQIKN
                     MSNSSRKFLRAFHVKLRVKNQEVLQQLKDNNYLLVANHSSLLDIFLLGAVDNYVFITS
                     TEMSETPIIGNIIRLGGCLYTDRKNKLNLPQEIKRFTDVLKKGFKVVLFPEKPGTDGT
                     KVNEFRPSLFQTAIDAACTVVPVCIRYLKLDGKPINGKNRFVVFWSKSINYLKYYWNL
                     LAHKMEVEINFLEPIEFDPNRDRSEL"
     gene            complement(1194..1579)
                     /gene="rnpB"
                     /locus_tag="PKM80_01395"
     ncRNA           complement(1194..1579)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKM80_01395"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1720..2529)
                     /locus_tag="PKM80_01400"
     CDS             complement(1720..2529)
                     /locus_tag="PKM80_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV92593.1"
                     /translation="MKTSNIIIIALAVLILNAGITLVLINQKEINAPASTTTIVDPSL
                     NQRHNEITAAVQLIEPAVVSVNVTKTQLVRPGFGFSIFDFFGDIPIQRQVVSIGSGVI
                     YDANGYIITNAHVIEGATEIKVVLPDKREFIANVVGVDNTYDVAKLKIDGNNFPYAKL
                     GTAKELLIGEWAIALGNPYGFMMSDPKPSVSVGVISALNRSFQPQDGHRYINMIQTDA
                     AINPGNSGGPLVNVKGEVIGINTFIVSGTGDNVGLGFAIPIDRVKSIIKIL"
     gene            complement(2538..3293)
                     /locus_tag="PKM80_01405"
     CDS             complement(2538..3293)
                     /locus_tag="PKM80_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HNV92594.1"
                     /translation="MKDQDLYLSVIKFPAFVSISLLIISAVFLIILFNVFAQPLLANW
                     VRTLFIILLAILIFIVLGWMITLFSTHTRLKPRWLQKLSKWMLFYIYYPLAKGVAHIT
                     FSSKKNLIESFLNFNNEIVLTDQKDISNTNILVLLPHCIQKEDCTIRITNDIIKCEEC
                     GGCDMAKIKKLITEQNVDAAVATGGFLERKMIEEKNPEVIVAVATAKELVNSLRDNWN
                     YPVYAVLNEQSITANEKNPVNISFIEFAIKQFK"
     gene            complement(3290..4264)
                     /gene="fmt"
                     /locus_tag="PKM80_01410"
     CDS             complement(3290..4264)
                     /gene="fmt"
                     /locus_tag="PKM80_01410"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNV92595.1"
                     /translation="MKIVFAGSSEFGIPALKKLKEKHQLLLIISQPDRPAGRNQRIKP
                     CPIARFAREQGWELYQPENINSPESVDKISKLYPELLVTASYASLIKKELRQIPTFGA
                     INLHPSLLPKYRGPTPIQTALLNGDHKTGTTIFRLTSRLDAGPILAQRELVILPEDNF
                     GSLKEKLANISAELLIEIIPAIKDNSLTERPQDENLASYTHKFSKKDFWINWDNKIKI
                     IFNQIRAFSPSPGARTSIHNTNLKILQTEIYDSSKNDKPGTIAGFIKNKGILVNCQDG
                     QLLITRIQAEGKKVMSAWAFQLGARLTAGESFVPFDKIFIENSYREEL"
     gene            complement(4281..4835)
                     /gene="def"
                     /locus_tag="PKM80_01415"
     CDS             complement(4281..4835)
                     /gene="def"
                     /locus_tag="PKM80_01415"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNV92596.1"
                     /translation="MSRKPKLLPIHIYGDKVLRLKSEEVQKIDENLLQFAEDLIATMY
                     QWDGVGLSAPQVGVSKRIIVIDPQIKDNPKKHSPMVMINPVIEESSGETETEEGCISI
                     PDIYAKVVRPSNITVSYTDLQNERHRINLSGYAAVIVQHEYDHLEGILFIDHLSTITR
                     LKIRNKLKELQKNTVDGINIRCEE"
     gene            complement(4844..5194)
                     /gene="yajC"
                     /locus_tag="PKM80_01420"
     CDS             complement(4844..5194)
                     /gene="yajC"
                     /locus_tag="PKM80_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNV92597.1"
                     /translation="MIYTLLQAATNSAQAKQSSGASGMSTLIILILLFVVMYFLMIMP
                     QQKKQKELQKMLDSLKENDKVVTTSGIYGRIVSIKPDKNTVVLEIDDNNHVRIEIQKG
                     AIASIVSSAEKANA"
     gene            complement(5208..5693)
                     /locus_tag="PKM80_01425"
     CDS             complement(5208..5693)
                     /locus_tag="PKM80_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNV92598.1"
                     /translation="MKSKSLSVETFLASVFGVGFIPYAPGTYGSIVAAAIYLLLPQML
                     FSGKGWLWYTGFLVILIALAVWLSSKAEKKLGPDAPQIVIDEVCGFFLSVLLLPKSFL
                     MAVYALVIFRALDIAKPFPIKISQKLPKGWGIVIDDLIAGVYTNILIHFIKLIAPKFF
                     G"
     gene            complement(5690..6952)
                     /locus_tag="PKM80_01430"
     CDS             complement(5690..6952)
                     /locus_tag="PKM80_01430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV92599.1"
                     /translation="MKKLILILLTSLLIFSLSAELVDRIVAKVGSDIILLSDVYKMMY
                     QMQLAGIPKEQISMDMALREIIEQKVISQKAKELNIKVDDDKIKRYAQNYLQELKSQY
                     PSPQEFQAALAQENLTEAELLEYFIEEIKNNAMTQQLIDLYVSSQVSVTEEEMREFYN
                     TTKDSMAVKPTTWETGMIKYKITASKATEDSVYKQMQDIKKRLDAGADFATLASEVSE
                     CPSKVRGGDLGYFSRGMMLKPIEDAAFSMDVGEISGIIRSENGYHILKLTDKRRDEVR
                     ASHILKLVQPTKSDTLAAYAIMERIRELYESGQSSFSDLALQYSTDPDVTKNKGIIGE
                     LTENEFPEPFKNYILSTPVGKMTPVLEKDGTLYIFVRLKEIPPRVFTYDEVKDKLYTY
                     LYQYKLQSAYNKWIDKLIKETYVEILEK"
     gene            complement(6974..7789)
                     /locus_tag="PKM80_01435"
     CDS             complement(6974..7789)
                     /locus_tag="PKM80_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92600.1"
                     /translation="MKRFLIAAIMVFILCSSCELKKEKEEFTPLAEVNGEVLTLEAFR
                     SLYTDEQWNKLSETQKRKEIENWVNLTILAQEADKEKISEESATKLRIDFAVKKVKAN
                     ALLARKLANLTISEEEMFNYYRLHQADFTKKLMEYNIQRILCPDENAAKSLLNKIKTG
                     EYDFNLAVLENSLEELKYKQGRMGFVSANSPDSLFWKAAHKLKINEPGIVTVNGKTYI
                     IRYTEQREGTQEATFVDYRNEIYELLLKEKKQQVYNDLVLELKMKNNNIYYYY"
     gene            complement(7793..9034)
                     /locus_tag="PKM80_01440"
     CDS             complement(7793..9034)
                     /locus_tag="PKM80_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92601.1"
                     /translation="MKTIQLQKMRLLLVATLFLAIILGGCINDHNPTGNNWSNVRPMS
                     FTTPLDIVAGYSFPGSGSIKGTETSLVCGNYNGIESVAFVRFTGLPSVGTFHIPEAYQ
                     DSTYLNLVLTRKFSESRNPVEVKIYKLNQSWAADSTNLIQDSNLSLITPQSYTITDSI
                     SSAGTQIKIPIPVAALEQWTSEADSLGLTLAIKTGENSYIEFKSRESGAGPKLHFLYR
                     TTGENPIEKKYDQYCNKDSYRIDEETAPIITNQWILSNIPPSRIYLYFPMNFSLFQDT
                     EGNPLNEIQLKRTTINHAELILYVKENPYYGTSTQYRLRADRVNDSLDINTAVAVLDE
                     QVASGIITQSYIRGDSLVVNVTPLIQAYTSGDKINYGIVIRSMQELINYGKIELWHFN
                     DSSTPAEKQPKLRVTYTPPFL"
     gene            complement(9293..10624)
                     /gene="dnaA"
                     /locus_tag="PKM80_01445"
     CDS             complement(9293..10624)
                     /gene="dnaA"
                     /locus_tag="PKM80_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNV92602.1"
                     /translation="MDQDIWQNILDKLEENINPQSFRTWFSETKLVDISDNELVVKVP
                     TQFAANYLNQNYTDTLGEIAYALYKQHYKIKFISPPHYVKNNPNQTAGYSDNRVMVNT
                     KLNERFSFEEFVVGRNNNFAYSAAKAVAEAPGYTYNPLFIYGESGMGKTHLMQAVGNY
                     VVKEGRNCSIYYTTSEEFTNEMIESIRANKMPEFRSKYRKVDLLLVDDVHFLSKKEGT
                     QEEFFHTFNALFDNRKQIVLTSDRPPKDIPDLEKRLVTRFESGLLCDLKNPDFETRVA
                     ILHKKAEPENIVLSDEVFSFIAESITSSVRALEGSLIRILAYASYNNLNPEELDVETV
                     RSILSDMISEQHKEITLDTITQEVCLAYNITMPQIVDKTRRQQISYPRQIAMYLANLL
                     IPQLSLKEIAEYYKRKDHTTVIHAKRMIENRFREDINFRSHIEQLIKNIKS"
     gene            complement(11189..11434)
                     /locus_tag="PKM80_01450"
     CDS             complement(11189..11434)
                     /locus_tag="PKM80_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92603.1"
                     /translation="MAVTKYDVKCYGFLLDYLEKNDPADEIEVISRLSYEKEWDSIPL
                     ELKQKILEIDKIILDKYASNFNYLLWKRFIQILKSHQ"
BASE COUNT         3650 a         2019 c         2001 g         4031 t
ORIGIN      
        1 atagttcgct tctatcacga ttagggtcaa actctattgg ttctaagaaa ttgatttcta
       61 cttccatttt atgtgccagc agattccagt agtattttaa ataattaatg ctcttagacc
      121 agaaaacgac gaaacgattt ttaccattaa ttggtttccc atccagtttc agataacgaa
      181 tacagacagg aacgacagtg caagcagcat ctatggcagt ctgaaagagg gagggacgga
      241 attcattgac ttttgttcca tcagtgccag gtttttcagg aaatagcaca actttaaaac
      301 ctttttttaa cacatcagtg aagcgtttga tttcttgagg gagatttaat ttatttttcc
      361 ggtcagtata aagacatcca ccaagacgga taatattacc aataatagga gtttcactca
      421 tttcggtgga ggtgataaat acataattgt caactgcacc aagcaaaaat atatctaata
      481 aagatgaatg attagctacc agcagatagt tgttatcttt aagttgttga agcacttcct
      541 gattctttac tctaagtttg acatggaaag cacgaagaaa ttttcgggaa gaattgctca
      601 tatttttaat ctgtaaccag cggcgtttaa ttggattttt agcaaaaaga cggataaaat
      661 aggaacgaca gacgaaagac cagagtagta tcatctgcca gatagtatga aataatttaa
      721 ctatccacat cattttatcc tcttttttat tataatcttc ttttctatat atatcgtaat
      781 ctgtcaagtt tttttctgct ggtttctacc ataacctcaa gaaaatatct tttatgttat
      841 agccttatat gactttatta agcttttata atttaattca ttccgagaat tatgaaaagg
      901 tataattatt gttttttacc gttataacaa attataaaaa catctctgca attttatttc
      961 ttctttcttg gcagtttatc cattactgat gtaaagatta tgtccaaatc ggacataagc
     1021 tggacatgag ttggacataa gctggacatg agctggacat gagcaaccca tcaagaggaa
     1081 acaataattg ggtggattta tctttcataa gctattggtt ctaaggaagg taggatttgc
     1141 tttacagttg gtagtatggt ttagggaaag atattaattt aaaaaaagaa aaactcacgg
     1201 gaaaataata agccggattc tgtacctcct tctatcagga ggttgatgtc cattcctcta
     1261 catccattct cacgaatgga ctttagctgc ctacccggaa gccataatcg tgcgggcaac
     1321 acttcttggc ttccctattt ggcattgcac cggataaggc tttcctggca aatcctgtca
     1381 ccaggatttc cggtggtctc ttacaccacc atttcaccct tacctgaaat agataattat
     1441 aaaataacta tttcaggcgg tttatttctg tggagcttgc tctgcattac tgcagcttcc
     1501 tgttaggaag tatcctgccc tttggtgtcc ggactttcct cccctacttt tcagtagcag
     1561 tggacatctc attttcccgt gagctttaag atataatata tgaaattgtt attctgacaa
     1621 gaaaaatatt tttcttgctt gcgcaaattt aattgagttt cttactactg agttatgaac
     1681 taattcagtt taagtaaatt ttagaaagaa gctatccagt tataggattt tgattataga
     1741 tttaactcta tctatgggta tagcgaatcc aagtccgaca ttatctcctg ttccagaaac
     1801 gataaaggta ttaatgccaa taacttcacc tttaacattt accagtggac caccgctatt
     1861 accaggattg atagcagcat cagtctgaat catatttatg tatctatgac catcttgagg
     1921 ctggaaactt ctatttagag cagaaataac accaactgaa acggatggtt tggggtcact
     1981 catcataaat ccataaggat ttcccaaagc aatagcccat tcaccaataa gtaattcttt
     2041 ggcggttccg agtttggcat aaggaaaatt attgccatct attttcaatt tagccacatc
     2101 gtaagtgtta tctacaccaa ctacattagc aataaattct cgcttatctg gaagcacaac
     2161 tttgatttcg gttgcacctt ctattacatg agcatttgta ataatataac catttgcatc
     2221 atagataaca ccacttccga tactaaccac ctgtctttga atgggaatgt ctccaaagaa
     2281 atcaaagatg ctaaaaccaa aacctggtct cactaactgc gttttggtca cattaacact
     2341 aactacagct ggttcaatta gttgaactgc tgcggtaatt tcattatggc gttgattaag
     2401 tgaagggtct acaatagtag tagtagaagc aggcgcattg atttctttct gatttatgag
     2461 taccaaagtg ataccagcat taagaataag tacagccaag gctataataa taatattaga
     2521 tgttttcata agccatccta tttaaactgt ttaatggcga attcaataaa agagatattt
     2581 actggatttt tttcattagc agtaatagat tgttcattta agactgcgta aacaggataa
     2641 ttccagttat ctctcagact attaaccagt tctttagcag tagcaacagc cacaataact
     2701 tctggatttt tttcttctat cattttccgt tctaaaaaac ctccggtagc aactgctgca
     2761 tcaacattct gttctgttat aagtttctta attttagcca tatcacatcc accgcattct
     2821 tcacatttaa taatatcatt agtgatgcga atggtgcaat cttctttttg aatacaatga
     2881 ggaagaagaa caagaatatt tgtgttagat atatctttct ggtcggttag gacaatttca
     2941 ttattgaagt ttaagaaaga ttcaatcaga ttctttttag aagagaaagt gatatgggca
     3001 acccctttag ccaagggata ataaatgtaa aaaagcatcc atttactaag cttttgaagc
     3061 cagcgcggtt ttaatctggt atgggtagag aaaagggtaa tcatccagcc taacacgata
     3121 aaaatgagta tagctaatag tataataaat agagttctaa cccaattagc cagcaaaggt
     3181 tgagcaaaga cattgaataa tataatcaga aagacagcac taataattaa aagactgatg
     3241 cttacaaaag cgggaaactt tataactgag aggtaaaggt cttggtcttt cataattcct
     3301 ccctgtaaga attttcaata aagattttat caaaagggac aaaactttcc cccgctgtaa
     3361 gtcttgctcc caattgaaaa gcccaggcag acataacttt tttaccttca gcctgaattc
     3421 tggtgattaa aagttgtcca tcctggcaat taactaatat acctttattt ttaataaatc
     3481 ctgcaatagt tcctggctta tcattttttg atgagtcata aatctcagtt tgaaggattt
     3541 taaggttagt attatgtata gatgttcttg cacctggtga aggtgaaaag gcacgtatct
     3601 ggttaaagat aattttaatt ttattatccc agtttatcca aaaatctttt ttactaaatt
     3661 tatgggtata agaagccaga ttttcgtctt gaggtcgttc tgttagacta ttatctttaa
     3721 tagcaggaat aatttcaatc aatagctcgg cagagatatt agccagtttt tcttttaagg
     3781 agccaaagtt atcttctggt aggataacca attctctttg ggctaagata ggacctgcgt
     3841 caagacgaga agttaacctg aaaatagtgg ttcctgtttt atggtctcca ttaagaaggg
     3901 cagtttgaat aggagtagga ccacgatatt taggaagtaa agatggatga agattaatag
     3961 ctccaaaagt aggaatctgt ctaagttctt tttttattaa agaggcatag gaagcagtga
     4021 ccagaagttc aggatataac ttgctaattt tatccacact ttcaggagaa ttgatgtttt
     4081 ccggttgata taattcccag ccttgttctc gtgcaaaacg agctatagga cagggtttaa
     4141 ttctttggtt tcgtcctgcg ggtctatctg gttggctgat aattagtagt aattgatgtt
     4201 tttctttgag tttttttaaa gcaggaatgc caaattcaga gctaccagca aagacaattt
     4261 tcattggata ttagcctctt ctattcttca caacggatat taatcccatc caccgtattt
     4321 ttctgcaatt ctttcaattt atttcttatt ttcaacctgg taatagtact tagatggtca
     4381 ataaacagga ttccttccag atggtcatat tcatgctgaa cgataacggc tgcatatcca
     4441 gaaagattaa ttctgtgtcg ttcattttgt aaatctgtat aagagacggt tatatttgaa
     4501 ggtctaacca ctttagcata gatatcagga atactgatgc acccttcttc ggtttcagtt
     4561 tcaccactac tttcttcaat aacagggtta atcataacca tcggagagtg ttttttaggg
     4621 ttatccttta tttgaggatc aatcacgata attcttttgc taacgccaac ttgaggtgca
     4681 gaaagtccaa ctccatccca ttgatacata gtagcgataa ggtcctcagc aaattgcaat
     4741 aagttttcat caattttttg cacttcctcg gacttaagtc tgaggacctt atcaccataa
     4801 atatggattg gtaataattt aggtttacgc gacattagat ttattaggca ttagcttttt
     4861 cagcagagct aactatactg gcaattgcac ctttctggat ttcaattcta acatggttgt
     4921 tatcatctat ttccaagaca acggtattct tgtctggttt gatagagacg atacgaccat
     4981 aaattccgga agtggtaaca actttgtcat tttctttcaa gctatccagc attttttgca
     5041 gctctttttg ctttttttgt tgtggcataa tcatcagaaa atacatcact acaaatagca
     5101 gaattagaat aattagagtg ctcattccgc ttgctccgga actctgtttt gcttgtgcgc
     5161 tattagtagc agcttgaagt agagtatata tcattttttt ctccttttta gccaaagaat
     5221 tttggcgcta taagttttat gaaatgaatc aggatattag tatagactcc tgcaatgagg
     5281 tcatctataa caatacccca acccttgggt agtttttgag aaatctttat aggaaatggc
     5341 ttggcaatat ccaaagccct aaaaataaca agggcatata cagccattag aaacgatttc
     5401 ggtaatagta atacacttag aaaaaatccg catacctcgt caatcactat ttgaggtgca
     5461 tcaggtccaa gctttttttc tgctttagaa cttaaccaca cagctaaagc aatcaagatg
     5521 acaagaaatc cagtatacca gagccatcct ttaccggaaa atagcatttg tggaagcaag
     5581 agatagatag ctgctgctac aatagaacca taggttccgg gagcataagg aataaatcca
     5641 actccgaaaa ctgaagccag gaaggtctct acagatagag atttagattt catttttcaa
     5701 gtatttcaac ataagtttct tttattaatt tatctatcca tttgttataa gcagattgta
     5761 acttatactg gtaaagataa gtgtaaagtt tatctttaac ttcatcataa gtgaaaacac
     5821 gaggaggaat ttctttaagc cggacaaaaa tatacagggt tccgtctttt tccaggacag
     5881 gggtcatttt tcccacagga gtagaaagaa tgtagttttt aaagggttca ggaaattcat
     5941 tttctgtaag ctctcctata atccctttgt ttttggtaac atcagggtca gtagaatact
     6001 gtaaggcaag gtctgagaaa gaactttgtc cactttcata aagttcacga attctttcca
     6061 taatggcata ggcagctaag gtatcagatt ttgtaggttg aaccaatttg agaatatgac
     6121 tggctcgcac ctcatctctt cttttatcag taagttttag aatatgatag ccattttcac
     6181 ttcggatgat cccagatatt tctcctacat ccatagagaa ggctgcatct tctataggtt
     6241 ttagcatcat acccctgctg aaatagccta aatcaccgcc tctaacttta cttgggcatt
     6301 cactaacctc actggctaaa gtagcaaaat ctgctcctgc atctaatctc tttttaatgt
     6361 cctgcatttg cttatacaca gaatcttcag tggctttact tgcagtaatt ttatacttta
     6421 tcattcctgt ttcccaggtg gtaggtttca cagccattga gtccttagtt gtattataga
     6481 attcacgcat ctcttcttcc gtaaccgata cctgtgagct cacatataag tctatcaatt
     6541 gctgagtcat agcattattt ttaatttctt caatgaagta ttctaataat tcagcttcag
     6601 tgagattttc ttgcgctaaa gcagcttgga actcttgagg agaaggatat tgagatttta
     6661 attcctgaag atagttttga gcatatcttt taattttatc gtcatcaact ttgatgttta
     6721 attctttagc tttttgagag ataacctttt gttcaattat ttcacgtaaa gccatatcca
     6781 tagaaatctg ttcttttggt atcccagcca gttgcatctg atacatcatt ttataaacat
     6841 cacttaatag gataatgtct gatcccactt ttgcgacaat acggtcaacc agctcagctg
     6901 aaagtgagaa gatgaggagt gaagttagta gtataaggat taattttttc attatattcc
     6961 ttttttatag agattagtaa tagtagtaga tattgttatt tttcattttg agttcaagta
     7021 ctaaatcgtt gtaaacttgc tgtttttttt cttttagcag tagttcatag atttcattac
     7081 gatagtctac aaaagtggct tcttgcgttc cttctctttg ttcagtgtag cgaatgatat
     7141 aagtttttcc attaacagta actatgcctg gttcgttaat tttaagctta tgtgctgctt
     7201 tccagaacag tgaatcagga ctattagcag agacaaatcc cattcttcct tgtttatatt
     7261 ttaattcttc taaagagttt tctaaaactg cgagattaaa atcgtattca ccggttttaa
     7321 tcttatttaa caggcttttt gcagcatttt catctgggca gagtatcctt tgaatgttat
     7381 attccatcag ttttttagta aaatcagctt gatgtaagcg ataataatta aacatttctt
     7441 cttcactaat agttaaatta gccagttttc gggcaagcaa agcattagct ttaactttct
     7501 ttacggcaaa atctatacgc agttttgttg cactttcttc actaattttt tctttatccg
     7561 cttcttgagc cagaatggta agattaaccc agttttctat ctcttttctt ttttgcgttt
     7621 cacttaattt attccattgt tcatctgtgt aaagagagcg aaatgcttcc aatgtaagaa
     7681 cttctccatt aacttctgcc agtggagtga attcttcctt ttcttttttt aattcacaac
     7741 ttgagcagag gataaatacc attattgctg caattaaaaa tcttttcata ctttagagaa
     7801 agggtggagt gtaagtaact cttagtttag gctgtttttc tgcaggagtg ctactatcat
     7861 tgaaatgcca taattctatc tttccgtagt taattaattc ttgcatagaa cggataacaa
     7921 tcccataatt tattttatcg ccagaagtat aagcttgaat taaaggagtt acattaacta
     7981 ctaaactatc tcctctgata taagattgag tgataattcc agaagcaacc tgttcatcta
     8041 agacagctac agcagtattt atatccaaag aatcattcac tctatcagct cttaatctgt
     8101 attgagtaga ggttccataa tagggatttt ctttaacata taagataagt tctgcatgat
     8161 tgatagtagt tctcttaagt tgaatctcat taagtggatt tccctctgta tcttgaaata
     8221 aagaaaagtt catagggaag tataaatata ttctggaagg tggtatatta cttaatatcc
     8281 attgattggt tattatgggt gcagtttctt catctatccg ataactatcc ttgttgcagt
     8341 attgatcata tttcttttct ataggattct cacctgtggt tctataaaga aaatggagct
     8401 ttggtccagc acctgattcc cgtgatttaa attctatata tgaattttct ccagttttta
     8461 tagccagagt caatcccaga gaatcagctt cagaagtcca ttgttctaaa gctgctacag
     8521 gaataggtat tttaatttga gttccagctg agctaataga atcagtgata gtatatgatt
     8581 gaggagtaat taaacttaaa ttggaatcct ggataagatt agtgctatct gctgcccagc
     8641 tttgatttag tttataaatc ttaacttcaa caggattacg agattctgaa aattttctcg
     8701 taagcacgag attaagatag gtagaatcct gataagcttc tggtatatga aatgttccca
     8761 cggaaggtaa acctgtaaaa cgcacaaaag ctacggattc tataccatta taatttccac
     8821 agactaaact ggtttctgtt ccttttatag aaccactacc aggaaaactg tagccagcta
     8881 ctatatctaa aggtgtagta aaagacatag gtcttacatt agaccagtta tttccggttg
     8941 gattatggtc attaatgcaa cctcccaata taattgccag aaataaggtt gctacaagga
     9001 gtaatctcat tttttgaagt tgaatagttt tcatatatta cctaatttat taaaatagat
     9061 tttcttttat agtagtttaa gtaggcaatg tgaataagtg gataaagggt caagtctttt
     9121 tttatcttat tgaaataaag cataatatct ttagagattt tttggggata agtagtgggt
     9181 aattttatac aaagttaatt tttttccact ctgccaggta agatatatct tatatactaa
     9241 cttatccaca ttaatccacc ggaattatcc acttatccac atatttttaa gtttatgact
     9301 ttatattttt gattagttgt tcaatatggg accggaaatt gatatcttca cgaaagcggt
     9361 tttcaatcat tcttttagcg tgaataactg tggtatggtc ttttctttta taatattctg
     9421 ctatctcttt tagagagagc tgtggtatga gtagatttgc cagatacata gcaatttgac
     9481 gtggatagga gatttgctgt cttctggttt tatcaacaat ttgtggcatt gtaatattat
     9541 aagccagaca gacttcttgg gtgatggtat caagtgtgat ttctttatgt tgttcagata
     9601 tcatatcgct aaggatagaa cggactgttt ccacatctaa ttcttcagga ttaagattat
     9661 tgtaagatgc ataggctaaa atacggatga gagaaccttc cagagcacgg acactagaag
     9721 taatggattc agcaatgaaa gaaaacacct catcactgag aacaatattt tccggttctg
     9781 cttttttatg aaggatagcc acccgagttt caaaatcagg attcttcaaa tcgcaaagta
     9841 gaccactttc aaacctggtt acgagtcttt tttctaaatc tggtatatct tttggaggac
     9901 ggtcagaggt taaaactatc tgtttacgat tgtcaaatag agcattaaaa gtatggaaaa
     9961 attcctcttg agtaccttct tttttggaaa gaaaatgcac atcatcaaca agcagtaaat
    10021 ctactttacg gtatttggaa cgaaactctg gcatcttgtt agcacgaata gattctatca
    10081 tctcattagt aaattcttca ctggtggtgt aatagataga acaattacgt ccctctttta
    10141 caacataatt tcctactgct tgcattaaat gcgttttccc cattccactt tctccataaa
    10201 taaaaagagg attataggtg tatcctggag cttcagctac tgctttagca gcagaatagg
    10261 caaaattatt gtttcgtccc accacaaatt cttcaaaact aaaacgttca ttgagcttgg
    10321 tattgaccat aacccgatta tccgaatagc cagcggtttg attaggattg tttttcacat
    10381 aatgaggagg agaaataaac tttatcttat aatgttgctt atacaaggca taagcaatct
    10441 ctcctaaagt atcagtatag ttttgattta aataatttgc cgcaaattgc gttggaacct
    10501 tgactacaag ttcattatcc gaaatatcca ccaactttgt ctcagaaaac caggttctaa
    10561 agctctgtgg attgatgttc tcctctaact tatctaaaat attctgccaa atatcctgat
    10621 ccatcttacc atcctaaagc taaaaaaatt tatttatcca caaaatcagg agaggcttgt
    10681 cctcagccta aacccgattc tttatttatt gctaatcaat aagataacta taatttaagt
    10741 tcaatttgta gttttcttta gatttagtta tccacacatt ttccacattc aattcccact
    10801 tcattcactt caaactcatc ctttaatttt tactgtggat agcttttcta aggtggaagt
    10861 tcttgtcaag tggctaaatc atttaactat ttataattac agggtttcat aatctataag
    10921 ttatgaaaaa taatattatt aaaacttgaa tcttggtttt aaaaaaatcc cttgccagaa
    10981 gtttatccac ttatgctaaa gtaaattaaa gaaattttaa attaatttaa tctcaattag
    11041 gaagagctgg aagcatttat gagctaatag atttgaaatt ataaagatag ggatttttga
    11101 ttaactcttc cataatatga agggaagaaa agttatcagg cagaaagttc aaattttaaa
    11161 attaagggaa ggaggtcgta aaatatctct attggtggct ttttagaatt tgaatgaacc
    11221 tcttccaaag tagataattg aaattagaag catatttatc caggatgatt ttatctatct
    11281 caagaatctt ttgctttagc tcaagcggta tggaatccca ttctttttca taagataatc
    11341 gagatataac ttcaatttca tcagctgggt catttttttc cagataatcc aacaaaaatc
    11401 catagcattt aacatcatat tttgttactg ccataataaa tcctcaagtt atcagtatat
    11461 ttaataaaca tcgtagaaaa cggttttatt atctaactta gacataatat ttattattca
    11521 aggattgatt ttaccttatg tgtcaaggaa gattatttca atagagagaa aagacctggt
    11581 agaaaataaa ttgacagaaa gataagaata gatataaagg gcaattgctt taatgaatgg
    11641 cacagaatag aattttatta tgagataaat gaaaaagtga aagaggtgaa ttatgaagaa
    11701 t
//
LOCUS       DAORHB010000024        11592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_4733_length_11592_cov_4.214094, whole genome shotgun sequence.
ACCESSION   DAORHB010000024 DAORHB010000000
VERSION     DAORHB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4733_length_11592_cov_4.214094"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /gene="hydF"
                     /locus_tag="PKM80_01455"
     CDS             <1..994
                     /gene="hydF"
                     /locus_tag="PKM80_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNV92604.1"
                     /translation="GQKRVQATQKILYRADIAIFVSDGRPFTKAELTMMDKIIGMELP
                     ILVVFNKSDIIEANPENMDLCLKKSLPYVSVSCITKDGIRECKDKLINLAPQVLKDNR
                     IIAADLVNPQSNIILVTPIDSSAPKGRLILPQVQTLRDLLDRNCVVTVVKETELKSAL
                     ENIKEPPSLVITDSQVIDMVAKIVPEEIPLTTFSILFARYKGELDILLEGINRLKSLK
                     DGDKILIAEACSHHIQKDDIGRVKIPNWMRSFSSKTLTFEVYAGHDFPDNLEEYAVCV
                     HCGGCMITSMEMNRRIMECHRRGVPITNYGLTISYLKGVLDRTIQPLMKEKKEQ"
     gene            991..1782
                     /locus_tag="PKM80_01460"
     CDS             991..1782
                     /locus_tag="PKM80_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNV92605.1"
                     /translation="MKVSVLQFEPRLLDKAYNLMRITDLLSSLKTDLVVMPELCTSGY
                     VFSTKEEVYSIAEVSGKGEAFLALSNIATKNNCSIVYGYPEVDGDKLYNSSMLIMPDG
                     DYHNYRKTHLFNREKLFFSPGDTGFQVFTTPAGVKIGMMICFDWQFPEAARTLALSGA
                     QIICHPTNLVLPWCQAAMKIRSLENRVFTITSNRIGTETNAGVTISFTGASQVLNTKG
                     ELLANLPVNKEGIATAEINPLEANDKTITERNDAFKDRRPELYRL"
     gene            1779..3782
                     /gene="ligA"
                     /locus_tag="PKM80_01465"
     CDS             1779..3782
                     /gene="ligA"
                     /locus_tag="PKM80_01465"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNV92606.1"
                     /translation="MNKEEIKAEIEELRAEIEKHNRLYYVLAKPEISDYEYDMLVKHL
                     QDLEAQYSEETIEESPTRKVGSDLAPGAKTIPHKVRMTSLENAYSLEEVLSFMNRTNN
                     DLGYNSEYCAELKIDGFSINLYYENGEFIYATTRGDGLEGEDVTENFRLIPEVPFTID
                     YKRPVEIRGEIYIPIQDFLALNETRRENELKTFANPRNAAAGSIKLKNSEEVKSRHLQ
                     ALFYALGYYESPLISKNEVVDKQTRLLDFLTELGFKTSKRALCNSYKEIEEFCLQVEQ
                     ERYTLPYDIDGIVIKVNNLEDQKKLGWTAKSPKWAIAYKFKPEEKETILESVSFQVGR
                     TGAVTPVANLKPVYISGSTVSHSTLHNADEIKRLDLHYGDTVLVVKSGEIIPKILSVN
                     ISKRPQNAKPVEFPVSCPVCYTPLERDEDGSIIYCPNANCPAQVQRKLEHFASREAMD
                     IYGLGSSLIARLIETGQIKSIPDIYHLDYDKIASMDRMGKKSAENLRKAIEASKTKNF
                     DRVLFALGIRHIGLITARTLAQHFKTIDALIAATDEDLLGVPDIGSIIAHSIIGFFSQ
                     KSNLELIDSLKAVGLQFAYTSSQRSNTLANKTFLITGTLSNYGRKEMESLIMEHGGKI
                     LSGVSKQLNYLIVGENPGSKLDKAHQLGTVKIINELELLDMLK"
     gene            3779..5107
                     /locus_tag="PKM80_01470"
     CDS             3779..5107
                     /locus_tag="PKM80_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNV92607.1"
                     /translation="MKILKRYLLKEHIAPFFIALMVVTFVLLSDRIIDLLNLIIEKKL
                     PWQIIFELFGLSLPYMLALSIPMAVLVATILAFGRMSVDREIIAIKSSGINIYSLIGP
                     LVIVALLLTGLMVYFNHWFLPETNHKLKNLTLKIAYYKPMTIIKEKEFTNFMDYTIFA
                     NEATDSLLTDVLIYDRSQTHFPRTVFAKTGNVIQKDNGNSLQIILNNGEMHERNEKEP
                     GKYQKTTFTNYVINVRNLGSNVDMFETGYRSDREMNYGQLVAAIKGHKEELTIKQQEI
                     VELNKRIELLKKSPPSFTRNMELQRLHSMQKMTSDRVEELKELISSLQVEYHKKFSIS
                     FAIIIFILIGIPLGLMTRTSGIGMSFSVSSVIFLIYYIALNAGEQLADKGNMPPFLSM
                     WLSNIAFLIIAIFLIISSIKEKKLIDMPTLMWRIKHYRNRKAPVPDEIVH"
     gene            5110..6198
                     /gene="lptG"
                     /locus_tag="PKM80_01475"
     CDS             5110..6198
                     /gene="lptG"
                     /locus_tag="PKM80_01475"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNV92608.1"
                     /translation="MKILDKYIIREFLRTYLIIFLSFVAIFVVIDVIDNLPRLIRNGA
                     TTQQATIYYLMRLPYLLVLTSPVTVLLTGLFMMSALSKHNESIAIRAAGISIKRAMLP
                     LFGIGLIISIAVALFGEYVVPWAETTKNYVYNVQIKGQQPDDQMLKARIHYQGKANDF
                     YYFGFFDGYKNNLKIIDLTRIDYKTHQISEHITASTADWNGSRWVLRDCEIRHFDNGK
                     QVFMAYYPQTEIVNLDVQPQDFIRITQKTLSLNFWQLREYIDRMKKLGDDPAREIVDL
                     HMKISFPLTNLIVIFFFIPIATSNIRSKGRGWIIMLGLVVCFVYLIVVRVCQSLGYNS
                     IINPVWAAWFPNLFFTILGLGFIYKAEI"
     gene            6482..8560
                     /locus_tag="PKM80_01480"
     CDS             6482..8560
                     /locus_tag="PKM80_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV92609.1"
                     /translation="MADSNRIAIHPILPVPDKKEIYFYWNGQKMQAKCGETIASALIA
                     NGISVFGHHHKDGSAQGIFCANGQCSQCSVIANGLAVKSCMTAVSENMIVQSLEGLPV
                     LPAVDNPLSFEPLEQIDTDVLIIGGGPAGLSAAIELGKENIPTLVIDDKNALGGKLVL
                     QTHKFFGSQADSSAGTRGIDIGKNLAAEIATYSSVDIWLNSTAVFVFSDKKVGVLKDG
                     VYKIVQPKRILNAAGAREKFLRFPGNNLAGIYGAGAFQTLVNRDLVRPTQRLFIIGGG
                     NVGLIAGYHSLQAGIDVVGLAEAMPVCGGYKVHSDKLKRFGVPIYTSHTILCANGDDT
                     VESITIAELDKNFQPVKGTEKTFTCDTILIAVGLNPLSEFTLEAEAAGLPIDAAGDAL
                     EIAEASSAMFNGKIAGVKIASILKGEKKNPIPDDWFIKAEELKSAPGKTNNYNTPEKE
                     EGVFPILHCLQEIPCNPCTTVCPTNSIHTEDGSLTAVPVYTGSCIGCGKCLLICPGLA
                     ITLVDYRKDKENPTISIVYEVSNYPVKVGDNKILNDINAKELGKYAITAVQDFPKQHT
                     QIVKFKVPKAIAKKVAGFQIQDANVSAPLTKPVLLEKTADEAMVCLCERVTAGEVRAL
                     IRKGITDMNLIKSITRAGMGPCGSKTCEVLIKGLLREEGIPEKEIAPITKRPLFIEIP
                     LEKFPNGSAK"
     gene            8557..9711
                     /locus_tag="PKM80_01485"
     CDS             8557..9711
                     /locus_tag="PKM80_01485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV92610.1"
                     /translation="MNKIYDVIVIGAGSTGLPTAYNLAKAGLKVLVIEAEHSPGQGNN
                     KKAIGGVRATHSDFGKINVCLRSIEILKNWSEEMGEDIGWLSNGYSYPAYNETDAKTL
                     KDLMQVQLGFGLNIKWLNPDEYSDIVPGIWKEGLQGSTYSPEDGSCSSLLVSAAYFEH
                     CKEEGVDFHFQEHITGFNIVSSKITKVITDKGIYSSAKVVNAAGSYAREVGQMANLDL
                     PVFPENHEAGITEPVERFFGPMVIDMRVAPGSSNFYFYQNSEGQVVFCITPEPPISGI
                     DTRSTSLFLPMCCKRMLTIYPRLRYLKVRRTWRGQYPMTPDGFPIVGIMDEAENLINA
                     VGMCGQGFMLGPGMGELVTRICLESTTEKDKEVLKSFDPYRQFTGQEAFK"
     gene            complement(9975..10574)
                     /locus_tag="PKM80_01490"
     CDS             complement(9975..10574)
                     /locus_tag="PKM80_01490"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNV92611.1"
                     /translation="MTNLYQNDIKHLTENGVFAGIDEAGRGALAGPVVIAAVVLDYNK
                     IIEGLNDSKQLSPKKREILFQQIVELALDYSIVEIGPDYIDKHNILQATLLGFKKAFL
                     QLKSKPEHCLIDGKDIHKDLKPYAKAIIKGDQLYAAIAAASILAKVHRDKLMLAYDEK
                     YPEYGFASHKGYGTAFHSKRIHSFGLSPIHRKSFKVPEV"
     gene            complement(10577..>11592)
                     /locus_tag="PKM80_01495"
     CDS             complement(10577..>11592)
                     /locus_tag="PKM80_01495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV92612.1"
                     /translation="QQFMQNKNPNFVLLSTNTQFVESLISMDSAHWEHFHPELNYRNL
                     CVGYNNPHRLLLHSQKQLLRLIRIPDSIYDLGGIRYSQQDDSTAYSFFATHYDQYMAH
                     VDYESWVNYVLSWFKQYSSLQLNKILELACGTANFSEQLVNRGYQVYACDNSIDMLHM
                     AAKKAVKPILYYASLTDPIPGRNYQLIICMFDSINYLTQAKQITKTLNEVSLALENGG
                     LFVFDISTLENSLDNFSDVSELYHYSDGLMLHQAWFESVRLMQKSALHFFKKDTIGYC
                     YQYEQHLQRVYFTNELIELIHNSPLKLRAIHSTDTKTNFYPKHLNSIDEKYSRLFFIL
                     EKI"
BASE COUNT         3750 a         1962 c         2272 g         3608 t
ORIGIN      
        1 gggacaaaaa cgcgttcagg ccactcaaaa aatactatat cgtgctgata tcgccatttt
       61 tgtttctgat ggcagaccat ttactaaagc tgaactgaca atgatggata aaattatcgg
      121 aatggaattg ccaatactgg tggtttttaa taaaagtgac ataatagaag caaacccgga
      181 aaatatggat ttatgcctaa agaaatccct gccttatgtg agtgtatctt gcataactaa
      241 agacggaatt agagaatgta aggataaact gatcaatttg gcacctcagg ttttaaaaga
      301 caatcgtatt attgccgctg atctagtaaa tccccaaagt aatataattc tggttactcc
      361 tattgattct tctgccccca agggtcgtct tattctgcct caagttcaaa ctctgcgtga
      421 tttgctggac cgaaattgtg tagtaaccgt tgtaaaggaa acagaactaa agtcagctct
      481 ggaaaacata aaagaaccac cttccctggt aataacagat tcccaagtaa ttgatatggt
      541 agctaaaatc gtacctgaag aaattccctt aaccactttt tctattcttt ttgcccgtta
      601 taaaggtgaa ttagatattc ttttggaagg cataaaccgc ttaaaatcac ttaaggatgg
      661 tgataagatt ttaattgctg aggcctgttc tcatcatatt caaaaagacg atatcggcag
      721 agttaaaatc cctaactgga tgagaagttt tagcagtaaa actcttactt ttgaggttta
      781 tgcaggacac gattttccag acaatttgga agaatatgct gtttgtgttc attgcggtgg
      841 ctgtatgatt acatctatgg aaatgaacag acgcattatg gaatgccata ggcgcggggt
      901 tcctattact aattacggat tgacaatttc ttatctgaaa ggtgttttgg atagaactat
      961 tcagccatta atgaaggaga aaaaagaaca atgaaagtat cagttttaca atttgaacct
     1021 cgtttattgg ataaggcata taacttaatg cgtatcacag acttgctaag ctctctaaaa
     1081 accgatctgg tagttatgcc tgaactttgt acaagcggtt atgttttcag cacaaaagaa
     1141 gaggtatatt ccattgctga agtttctggt aaaggagaag cgtttcttgc tttatctaat
     1201 atagccacaa agaataactg cagcatagtt tatggttatc ctgaagttga tggtgataaa
     1261 ctatacaatt ccagcatgtt aataatgccc gatggtgatt accataacta ccgaaaaacc
     1321 catttgttca accgtgaaaa gctgttcttc agtcctggag atacaggttt tcaagttttt
     1381 actactcccg ctggagtaaa aatcggaatg atgatttgtt tcgattggca atttcctgaa
     1441 gcagcaagaa ctcttgccct atcaggtgct cagattattt gtcatccaac gaatttagtt
     1501 ttgccttggt gtcaggcagc aatgaaaatt cgcagtttag aaaatagggt atttaccata
     1561 acctcaaatc ggatagggac agaaaccaat gctggagtta caattagctt tacaggtgca
     1621 agccaggttc ttaatacaaa aggagaactt cttgccaatc ttcctgttaa caaggaagga
     1681 attgcaactg cagaaataaa tccccttgag gcaaatgaca aaactataac agaacggaat
     1741 gatgctttta aagatagacg accggagctt tatcgcttat gaataaagaa gaaataaaag
     1801 ccgagataga agagcttaga gctgaaatag agaaacataa ccggctttat tatgtattgg
     1861 caaaacctga aatcagcgat tacgaatatg atatgctggt taagcatttg caggatttgg
     1921 aagcgcaata ttctgaagaa accatagaag aatcccctac tcggaaagtt ggcagtgatc
     1981 tagctcctgg agctaaaacc attcctcata aggtacgaat gacaagcttg gaaaatgctt
     2041 actccttgga agaggtttta tcgtttatga acaggacaaa taatgatttg ggttataata
     2101 gtgaatattg tgctgagctt aaaattgatg gattcagcat caatttgtat tacgaaaacg
     2161 gagaattcat atatgctaca actcgaggag acggtttaga aggtgaggat gtaacagaga
     2221 acttccgttt gattccagaa gtacctttta ctatcgatta taaaagaccc gtagaaatca
     2281 gaggtgagat ctatattcct atccaggatt ttttagcact gaatgaaacc cgaagagaaa
     2341 atgagctaaa aacctttgct aatccgcgta atgctgctgc tggttcaatc aaacttaaaa
     2401 attctgaaga agttaaatct cgccatttgc aagctttgtt ttatgctctt ggttattatg
     2461 aatcaccctt gatttccaaa aacgaagtgg tagataaaca aacacgctta ttagattttt
     2521 taacagagct tggctttaaa accagtaaaa gagctctatg caattcctat aaagaaattg
     2581 aagaattttg tttgcaagta gagcaggaac gttatactct tccgtatgat attgatggaa
     2641 ttgtaattaa ggttaataat ctggaagatc agaaaaagct tggttggacg gctaaaagtc
     2701 ccaaatgggc aattgcttat aaatttaagc cagaagagaa agaaacaata ttggaatcag
     2761 tcagcttcca agttggcaga accggtgcag ttactcctgt agcaaattta aaacctgtat
     2821 atatttcagg aagcactgtt tctcattcta ctttgcataa tgccgatgaa ataaagcgac
     2881 tcgatttaca ttatggggac acagttttgg tagttaaaag cggtgagatt attcctaaaa
     2941 ttctttcagt aaacatcagt aaacgcccgc aaaatgctaa accagttgaa tttcctgtta
     3001 gttgcccagt atgttataca cctttagaac gcgatgaaga tggaagcata atttactgcc
     3061 caaatgctaa ttgtcccgct caagtacaac gaaaactgga acattttgct tctcgtgagg
     3121 caatggatat atatggctta ggtagttctt taattgcccg tttgatagaa acaggtcaga
     3181 taaagagtat tcctgatatc tatcaccttg attatgacaa aattgcctct atggatagaa
     3241 tgggaaagaa atctgcagaa aatttacgga aagctattga agcatccaaa accaaaaatt
     3301 ttgatagagt cctttttgct cttggaatta ggcatatagg actaattact gctcgaacac
     3361 tggctcaaca ttttaaaacc attgatgctc taattgcagc aaccgatgaa gatcttttag
     3421 gtgtccctga tattggttct attatagctc attccattat tggctttttc tctcaaaaaa
     3481 gcaatctgga actgattgac tctttgaaag cagtcggatt gcagtttgcc tataccagct
     3541 cacaaaggtc taatacattg gcaaataaga cttttctgat aactggtact ctgtctaatt
     3601 atggtaggaa ggaaatggaa agcttaataa tggaacacgg gggtaaaata ttaagcggtg
     3661 taagcaaaca actcaattac cttatcgtag gtgagaatcc tggttctaaa ctggataaag
     3721 cacaccaact gggaacagtt aaaataatta atgaattaga gcttttggat atgctgaaat
     3781 gaaaatcctg aagcgatatc ttttgaaaga gcatattgct ccatttttta ttgctctgat
     3841 ggtagttacc tttgttttat tgagtgatcg aattatcgat ttattgaacc tgattattga
     3901 aaagaaactg ccttggcaaa taatatttga actatttggt ctttctctgc cttatatgct
     3961 tgctctttct attcctatgg ctgtgcttgt tgcaactatt cttgcttttg gaagaatgag
     4021 tgtagataga gaaattatcg ctattaaatc cagcggaata aatatttatt cgctaattgg
     4081 ccctttggtt atagtagctc tgttattaac cgggttaatg gtttatttta accattggtt
     4141 tcttcctgaa actaatcata aattgaagaa tttaacactg aaaattgctt attataagcc
     4201 aatgacgatt atcaaagaga aagaatttac gaactttatg gattatacta tcttcgccaa
     4261 tgaagcaacg gattctttac taacggatgt tctaatatat gatcgcagtc aaacacattt
     4321 cccccgtact gtttttgcca aaacaggaaa tgtaattcag aaggataatg gcaattctct
     4381 tcagataata ttaaataacg gagaaatgca tgaacgcaat gaaaaagagc ccggtaaata
     4441 tcagaaaacc acttttacca actatgtgat aaatgttcgt aatttaggta gcaatgttga
     4501 tatgtttgaa acaggttatc gtagcgatag agaaatgaac tatggccagt tagttgctgc
     4561 tattaaaggt cacaaagagg aacttacaat aaaacaacaa gaaatagtag aattgaataa
     4621 acgcatagaa ctgcttaaga aaagccctcc aagctttact cgcaatatgg aattacaacg
     4681 cttgcattca atgcaaaaaa tgacaagtga ccgagtagaa gaactaaaag aactgattag
     4741 ctctcttcag gttgaatatc ataagaaatt ttctatctct ttcgctatca ttatctttat
     4801 cttgataggt ataccacttg gtttaatgac tcgaaccagt gggataggta tgtccttttc
     4861 ggtttcttcg gtaattttcc taatttatta tatagctttg aatgcaggcg aacaacttgc
     4921 tgacaaagga aatatgccac cttttctttc aatgtggtta tccaatattg cgtttttaat
     4981 cattgccatt tttttgatta ttagctccat taaagagaaa aagttgatag atatgcccac
     5041 tctgatgtgg agaattaagc actaccgtaa cagaaaagca cccgttccag atgagattgt
     5101 acactaaaga tgaaaatcct cgataaatat attatccgtg aatttttgcg cacctatctg
     5161 attatctttc tatcttttgt cgctattttt gtggtgatag atgttattga taatttgccg
     5221 cgcttaattc gtaacggtgc taccactcaa caggcaacta tatattattt aatgcggtta
     5281 ccttatttgc ttgtcttaac ttctccagta acagttctgc taactggttt atttatgatg
     5341 agtgcacttt ctaaacataa cgagagtata gctatccgtg ctgctggaat tagtataaaa
     5401 agagctatgt tgcctctttt tggtattggt cttattatta gtattgcagt tgctctattt
     5461 ggagaatatg tagtcccttg ggctgaaacc accaaaaatt atgtttacaa tgttcaaata
     5521 aagggacagc agccagacga ccaaatgctg aaagccagaa tacactatca gggcaaagcg
     5581 aacgattttt attactttgg tttttttgat ggttataaga acaatctaaa aattatagac
     5641 cttactcgta ttgactataa aactcatcaa ataagtgaac acataactgc ctctactgct
     5701 gattggaatg gaagtagatg ggtgttaaga gattgtgaga ttaggcattt tgataatggc
     5761 aaacaagttt ttatggctta ttatcctcag actgaaattg tcaatttaga tgttcaacct
     5821 caggatttta taaggattac gcaaaaaacc ctatccttaa atttttggca attgagagaa
     5881 tatatagaca ggatgaaaaa gctgggtgat gatccagccc gtgaaattgt agatctacat
     5941 atgaaaatat cctttccgct cactaaccta attgtgatct ttttctttat ccccatagct
     6001 acttctaata ttcgttctaa gggtcgcggt tggatcatta tgctgggctt ggtggtttgt
     6061 ttcgtctatt taattgttgt aagggtctgc cagagtttag gttataatag tataattaat
     6121 cctgtttggg ctgcctggtt cccgaatttg ttctttacta tccttggttt aggattcata
     6181 tataaagcag aaatttagca attctgtagt gctttttacc ataacagtca ttagaagaat
     6241 gggaatgcag ttagaatttg cttaaacttc ttatttctgc aatagattat aaaattggag
     6301 ctatctatct tgctgtagat ataaaaaaca acaagtgcta ctgctaaatg tgccacaatt
     6361 actgcttgac aaactaccaa ggaaaaaatt gaagggaaga taaataatat tagaaaaagg
     6421 gaatatgctc tgctgactcg taaagcaaca tatctcttta aagaataagg agtttgaaca
     6481 aatggcagat agcaatcgta tcgccattca tcccattttg cctgttcctg ataaaaaaga
     6541 aatatacttt tactggaatg ggcaaaaaat gcaggcaaag tgcggagaaa caattgcttc
     6601 agctcttatt gccaatggta tttcggtttt tgggcatcac cataaagatg gcagtgctca
     6661 aggaattttt tgtgcaaatg gacaatgttc acaatgtagt gtgatagcta atggtttagc
     6721 tgtaaaatct tgtatgacag ctgtcagtga aaatatgatc gtgcaatcat tggaagggct
     6781 tcctgtttta cctgcggtag ataaccctct atcgtttgaa ccgttggaac aaattgatac
     6841 cgatgttctt attattggag gaggtccagc aggactttct gcagctatag aactcggcaa
     6901 agaaaacatc ccaaccttgg ttattgacga taaaaatgcc ttaggtggca agcttgttct
     6961 acaaactcat aaattctttg gttctcaagc agatagcagt gcaggaacaa gaggtattga
     7021 tattggcaaa aatcttgcag cagaaattgc tacatatagc agtgtagata tatggctgaa
     7081 tagtactgct gtttttgttt tcagcgacaa aaaggtagga gttctaaaag atggagttta
     7141 taaaattgtt caacccaaac gtattttgaa tgctgctggt gccagagaga aattcttaag
     7201 atttccaggt aacaatttag caggaattta tggtgcaggt gcttttcaaa cacttgtaaa
     7261 tcgggatctc gtacgtccta cccaacggct ttttataatc ggaggaggca atgttggttt
     7321 aattgctggt taccattcct tgcaagcagg tattgatgtt gttggcttag cagaagcgat
     7381 gcctgtttgt gggggttata aagttcattc tgataaactg aaacgctttg gagtaccaat
     7441 ctatacttcc cataccattc tttgtgccaa tggagatgat acagtagaga gtattactat
     7501 cgcagaatta gataagaact tccagcctgt aaaaggaact gaaaaaacct ttacctgtga
     7561 tacaatttta attgcagtag gacttaatcc tttaagtgaa ttcactttag aggcagaagc
     7621 agcaggttta ccaattgatg cagccggcga tgcattagaa attgcagaag caagttctgc
     7681 aatgttcaat ggaaaaatag ccggtgtgaa aatcgcttcc atcctaaagg gagaaaaaaa
     7741 gaatcctatt cctgatgact ggtttataaa agcagaggag ctgaaatcag ctccaggaaa
     7801 aacaaataac tataatactc ccgaaaaaga ggaaggggtc ttccctattt tgcattgcct
     7861 tcaggaaatt ccctgtaatc cttgcacaac tgtttgccct accaatagta ttcacacaga
     7921 ggatggttct ttaacagctg ttcctgttta tactggctct tgcattggct gtggaaaatg
     7981 tttgttaatt tgtccaggtt tagctattac tttagtggac tatagaaaag acaaagaaaa
     8041 tccaactatc agtattgtat acgaagtaag caattatcct gttaaagtag gggataataa
     8101 aatcctgaac gatataaatg ccaaggagtt aggtaaatat gctattactg cagttcagga
     8161 ttttcccaaa caacatactc agattgtaaa atttaaagtt ccaaaagcaa ttgctaaaaa
     8221 agttgctgga ttccaaattc aggacgcaaa tgtttctgca ccattaacaa aaccagttct
     8281 tttagaaaag acagctgatg aagcaatggt ttgtttatgt gaaagagtta cagcaggtga
     8341 agtacgagct cttataagaa aaggaattac agatatgaat ttgataaaat ctataaccag
     8401 agccggaatg ggaccttgtg gctctaaaac ttgtgaggta ctgataaaag gtttacttcg
     8461 tgaagaaggc attccagaaa aagagattgc tcccattact aaaagaccgt tatttataga
     8521 aattcctctg gagaaatttc ccaacgggag tgcaaaatga ataagatata tgatgtaata
     8581 gttattggag caggttcaac tggactccca accgcttata atttagcaaa agctggatta
     8641 aaagtcttag ttatagaagc agaacatagc cctgggcagg gaaataacaa aaaagctatt
     8701 gggggagtac gtgccaccca ttctgatttt ggtaaaataa atgtatgttt acgatcaatt
     8761 gagatattaa aaaactggtc agaagaaatg ggcgaagata ttggttggct ttccaatggt
     8821 tacagttatc cagcttataa tgaaactgat gccaaaacct tgaaagacct gatgcaagtt
     8881 cagctcggtt ttggacttaa tataaaatgg ctaaaccctg atgaatatag tgatatagtt
     8941 cccggaatct ggaaagaggg tttacagggt tctacctatt ctcccgaaga tggctcttgc
     9001 tcttctttac ttgtttctgc agcttatttt gagcattgta aagaagaagg tgtagatttc
     9061 cattttcagg aacatatcac cggattcaat attgtttcca gtaaaattac aaaagtgatc
     9121 acagataaag gcatttattc ttctgctaaa gttgttaatg cagcaggaag ttatgctcgc
     9181 gaagttggtc aaatggctaa tctggattta cctgtatttc ccgaaaacca tgaagcaggc
     9241 ataactgaac cagtagaaag attttttggc ccgatggtta ttgatatgcg tgtagcacct
     9301 ggaagttcta atttctattt ctatcagaat agtgaaggac aagttgtttt ttgcataact
     9361 cctgaacctc ctatttctgg aattgatacc cgctcaactt ctctcttttt acctatgtgc
     9421 tgtaaaagaa tgctaactat ctatccccgt ttacgatatc taaaagttcg ccgaacctgg
     9481 agaggacagt atcctatgac cccagatgga tttcctatag tgggaattat ggatgaagca
     9541 gaaaatctga ttaatgctgt tggaatgtgt ggtcagggtt ttatgcttgg tcctggaatg
     9601 ggtgaattag ttactcgtat ctgtttagaa agcactacag aaaaggataa agaagttcta
     9661 aagagttttg atccctaccg tcagtttacc ggtcaggaag cattcaaata gccctttcgt
     9721 attaagggtt taagaaagct aagtccaaaa ctacttgtat aagtctaaag ccatacaagc
     9781 aaagagagaa aagaattact ccgagctaaa aactaaatgt tgatagagaa cgcagtttag
     9841 cttgattcct gtttaattct ctttgataga tatgcggtgt cttctcttgg atatttaatg
     9901 agaggagtca ccgcattatt atggtattta ttctgtgggt ttatatccca aaacttcata
     9961 atcctcacag ccttttagac ctcaggtact ttaaaacttt tccggtgaat aggagatagc
    10021 ccgaaactat gaattctttt actgtggaaa gcagttccat atcctttatg ggaggcaaaa
    10081 ccgtattccg gatatttttc atcataagca agcatcagtt tatctcgatg gactttagct
    10141 aaaatagagg cagcagcaat tgcagcatag agttgatcac ctttaataat tgcttttgca
    10201 taaggtttca ggtctttgtg tatatctttt ccatcaatca agcaatgttc cggtttactt
    10261 ttcagctgta aaaacgcttt cttgaaaccc aaaagagttg cctgaaggat gttgtgttta
    10321 tctatgtagt ctgggcctat ttctacaatg ctataatcaa gagcaagttc cacaatttgc
    10381 tgaaagagaa tttcgcgttt tttgggagaa agttgtttgg aatcgtttag gccttcaata
    10441 attttgttat aatctaaaac tacagctgca ataacaaccg gtccagctaa ggcacctcta
    10501 cctgcttcat caattccggc aaagacacca ttttccgtaa gatgttttat atcattctga
    10561 tagaggtttg tcattttcag attttttcca ggataaaaaa caggcgacta tatttttcat
    10621 ctatgctgtt taagtgtttt ggataaaagt ttgttttagt atccgtgcta tgaatagctc
    10681 ttagtttcag aggagaattg tggataagct caatcagttc attagtaaaa taaacccttt
    10741 gtaagtgttg ttcatattga tagcaatagc caattgtatc ctttttaaaa aagtgcagtg
    10801 cagatttttg cattaatcgc acactttcaa accatgcttg atgaagcatt aaaccatcag
    10861 aataatggta tagttcagag acatcagaaa agttgtctaa actgttttcc agagtagaaa
    10921 tatcaaaaac aaataaacct ccgttttcta aagccagcga aacttcgttc agtgttttgg
    10981 ttatttgttt tgcctgagtg agataattga tactatcaaa catacaaatt atcagctgat
    11041 aattccttcc gggaatagga tctgttaaag aagcataata taatataggt tttactgctt
    11101 ttttagctgc catatgcaac atatcaatag aattatcgca agcataaacc tgatatcctc
    11161 tatttacaag ttgttctgag aaatttgctg tcccgcaagc gagctctaag atcttattaa
    11221 gctgtaaaga ggaatattgc ttaaaccagg aaagaacata attcacccat gattcataat
    11281 ccacatgagc catatattga tcatagtggg ttgcaaaaaa agaataggca gttgaatcat
    11341 cctgttgaga ataacggatc cctcctaaat cataaatgct atcaggtatg cgaattaacc
    11401 ttagtagctg tttttgggaa tgtaacaata accggtgagg attattgtat ccaacacaga
    11461 gattacgata atttaattct ggatgaaaat gttcccaatg tgcactatcc atacttataa
    11521 gggattccac aaattgagta ttagtgctta gtagaacaaa attcggattt ttattctgca
    11581 taaactgttg ga
//
LOCUS       DAORHB010000025        11300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_4931_length_11300_cov_4.197777, whole genome shotgun sequence.
ACCESSION   DAORHB010000025 DAORHB010000000
VERSION     DAORHB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_11300_cov_4.197777"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..79
                     /locus_tag="PKM80_01500"
     CDS             <1..79
                     /locus_tag="PKM80_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNV92613.1"
                     /translation="GASIKKREETHRMAEANKAFAHLRW"
     gene            197..273
                     /locus_tag="PKM80_01505"
     tRNA            197..273
                     /locus_tag="PKM80_01505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:231..233,aa:Arg,seq:ccg)
     gene            369..2132
                     /locus_tag="PKM80_01510"
     CDS             369..2132
                     /locus_tag="PKM80_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNV92614.1"
                     /translation="MNEVLLTLESVSFLYGDRIILQDVSFGIHNSEKIGIVGINGSGK
                     TTLLNIIGGIIKPSTGIVTSRKDLKIGFLEQNPQFIPELSVLQHTIPTQGEIKDEYHY
                     KSILSRLGIEDYNQKMILLSGGQRRKADLARVLALEPDLLILDEPTNHLDLDTIEWLQ
                     NYLADTNKAIIFVTHDRYFLDAVSTKIIEIERTRLFYYEGNYSAYIHGKLIRATDSQR
                     KETRRQAQLKKELDWLNRGAKARTSKPKNHIDRVKELLSKSYLISNQDLDISFQIDRM
                     GKTILELHRLSKSYGSRVLFEDLDHNFQNRERIGIIGPNGCGKTTLLKIIAGEDEPDI
                     GVVKAGINTHFAYYKQDEDLFDPNTTVYEYIAQFAEVIKTENGNKITAAEMLKRFLFD
                     GKMQQMKLGSLSGGERKRLYLLKALMFGANFIIMDEPTNDLDIRTLEILEDYLDAFKG
                     CLLIVSHDRFFLDRTVDYLFIFQEGKIRKFAGNYSDYLLVKRFEQEEDTTKKKPAILR
                     PKRISKGLTYNEQREFSQTEKDIEKIENQLIELEDKLNDFSQTLTPNDYHQISITILK
                     LEEQHQNLLNRWLELSDKQDN"
     gene            2181..2753
                     /locus_tag="PKM80_01515"
     CDS             2181..2753
                     /locus_tag="PKM80_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV92615.1"
                     /translation="MISELVYRNRSYRRFYESKRITHSQLIQIMETARFIPSAGNLQK
                     LCYYLIEERSECDKMFPHLKWASYLKKWNGPEPGERPSAYIIILVPKSCSNMVYIDTG
                     IAAQTILLTAVDNGLGGCLLASVDKDAVHKIFLLPDNMDIVLVIALGYPAETVVIEDV
                     TDQDKIEYWRDENTVHHVPKRKLSDLILNY"
     gene            2823..4163
                     /gene="xseA"
                     /locus_tag="PKM80_01520"
     CDS             2823..4163
                     /gene="xseA"
                     /locus_tag="PKM80_01520"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNV92616.1"
                     /translation="MENVIVYSVYEVTRQIRQVIESSIEPLYVMGEISGFVHHSSGHI
                     YFNLKDENSSLRCTFFKGANYNLTFKPEDGMQVICYGKLTVYEKGGTYNLNVLKMQEA
                     GQGLLQQKFEILKKKLEKEGLFATAHKKPIPKYPERIGIVTSPTGAALQDILNIISRR
                     YPVEIIVYPALVQGLEAPPQIINGIKYFNTTKDVDFIILTRGGGSQEDLFCFNDESLV
                     RAIFDSDLPIISAVGHEIDFTLADFVADLRAPTPSAAAELAVPDKKDLLNLLNSLKKR
                     LDLTVQNKLEMKHKPIMNYQLQLTQYHPEKVWREYQQRFDLAEMAMKNALKQLPDKRL
                     QFEMRTQTIMQQVTSKIDLCIAQAKANSNLAWHQLNDKTLNLLHNKANHLNSLGNILE
                     QLSPRSIQRKGWIMAFKDGKLISSVKQITKGNHLNLDFIDGIADSIIERVENKE"
     gene            4175..5608
                     /locus_tag="PKM80_01525"
     CDS             4175..5608
                     /locus_tag="PKM80_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNV92617.1"
                     /translation="MIFSLLFLLKLSAEIRSVWVLPWDITTESAIDQVINTAVDCNQN
                     ELLVEVRYRADALFDTSQGAYLYPNPEPVSYILNNSSFDPLTYIIQQAHNNGLAVQAW
                     IVVFNATQTDHYLIRQNYIYNNHRNWITYDKNGYQMNNGYRYGYFIDPGIPEVQEYLL
                     NIVGNIAAGYPELDGIHLDYIRYPDADLGYHPISQELYRAYCLNQMDITYNEWRIMQI
                     NNFVEKAYHLIKEINPSLLLTAAVFADIADANVSYAQDWQSWLKNGIIDRVYPMAYDI
                     NYAKFSKHTEQMKLLNFDSKIVIGLRAWNENGSSLALGKGASYNVQNVARKIDLAREQ
                     GFAGISLFSYSGLIKGNAWAQLTRLSYSEKAEYPFEKVSTPLVNQSVTELKQPPDVKL
                     LRLNNEILIQLQIPSEGLWNWELYKESFLLQHNRYYLKGNNIDILNVINPNICASIEP
                     GQYIVQLYQENSSFKYFIPVELKIANE"
     gene            5601..7064
                     /locus_tag="PKM80_01530"
     CDS             5601..7064
                     /locus_tag="PKM80_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNV92618.1"
                     /translation="MSDLPKQAGILSATEFFRLAIKSIIGIALARIITPAELGSYRQL
                     FLIYTTFSTLLLLGIPQSMLYFLPKAENAEKQRLLITRSLNLISILALIFALCLLIFR
                     NFIAQIFHNPQLGNLLIIYALYPLFLFVTQLYSSIMMGLKQPLRAAKFTIFSVLSDFI
                     FIVSAAFFTRNLTIIIWAVIASAFLQWLYAQITLNEFKEKKVRFDFASVKEQMNYALP
                     LGLSSIIGMLSIQLDKLMISGFFPPEKFAVFSLGATEFPIVGILANSINSVLLPHLSS
                     TNTATMGELYSGAVRKNAILVFPLMALCFIFAEPIFILLYGKIYAEAAVYFRIYLFLM
                     PLRIATYGILFQAFGKTKFIMYNSLFVLIANAVLNYILIINLGMKGAAIATVIVTWLS
                     VMIYLIQIAKVLKLKLLDFFPIGKITKNALVTIISLFLSFLIIWLGKQNLLFVAIGGI
                     VFILSYLLLGRKTGVILDYDVQLAKELINDLKGKFKK"
     gene            7061..7510
                     /locus_tag="PKM80_01535"
     CDS             7061..7510
                     /locus_tag="PKM80_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92619.1"
                     /translation="MNYYAESTWKNYDPEQQADIRIAKLVSLFPENMHYYKYTNCGLT
                     YNAYGHLQSFSKNTLNSLLTLEFSFTAQDILCPLNRDFIPFLLDIKQHWLKQWFNPPS
                     GTIDSNCGKSEFLNKKSLFTKSINYFINMSTKPQPYWFMELFVWREN"
     gene            7513..8784
                     /locus_tag="PKM80_01540"
     CDS             7513..8784
                     /locus_tag="PKM80_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV92620.1"
                     /translation="MKNLLFITYHFPPTGGSGVQRGLKFAKYLPQFGFRPIVLCADYR
                     CLKQPKDYTLLKELPPSVKIYRTFTLDANWFFKLLWGLKLNKVVTFLQRYILIPDTEV
                     LWLPFAFLKLRKILDANHIDLVLISAPPYSSLFLAKYCKHRYQLKVCVDFRDPWSFGI
                     GRKYLKPPSWIANKENNWEKEIVTLADKIICVNDKMVDEFRQLYTKIPAVKFCSITNG
                     YDEEDFSISTPLKKSDKFKIVYTGSFYDARRPLILWQALWELIEEGKINPQKITVNIY
                     GRNTKNFVLGEYQDNLLIQKIVHFYGYTPHTQIIKILLSADVLLLYSGFGNTEQLNSP
                     AKLFEYLRSGKPVFAIIDPESSAAEILLPANTIFLANSASLSSIKNTLSELYTQWEEG
                     KLKVSPDWEYIKNFERKALTARLAEVLSSIT"
     gene            8784..9953
                     /locus_tag="PKM80_01545"
     CDS             8784..9953
                     /locus_tag="PKM80_01545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV92621.1"
                     /translation="MKILIIPSWYPYPKKPYAGKFFIDQAKALSENSNWQFEILNWGQ
                     NEFQMQIRYPLSSIAKLFAYLKAKPSSRNFNSNLKELFIPHITWTSKLLSGNIKTLIK
                     KMELIPKPDIIHSHVTFPAGFVAMLFAEKWKIPYIITEHSGPFPFPEFVSNNRISEKI
                     TLPLQKAAAVISVSRYLQKQILSTCLVKSIVIPNLIDTEFYKPVSISEKRRRFSFFSL
                     SALIPAKGVLDLIEAFRLAVNKGMDADLYLGGSGYLAPKLRKLIIQYHLENRIHLLGY
                     LTPQQALEQFNKCNCYILPSHIESFSMVLLEAMACGKPIIATDCGGPKDIITETTGVL
                     IPPQKHDVMAIEMLKMANNSNKYNPDSIREWCLKNYSPHIICAQISRVYESITGS"
     gene            complement(9962..10330)
                     /locus_tag="PKM80_01550"
     CDS             complement(9962..10330)
                     /locus_tag="PKM80_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV92622.1"
                     /translation="MTKILVVEDDVSFRNVLVQMLKNSGYFVQQAGDGDQALEICCEF
                     DLDIVITDIIMPNKEGLETIQDLMAIKPGIKIIAMSGGGKYGPYSYLPLALKLGAKAV
                     LEKPFMRNEMIEVIEKVLNS"
     gene            complement(10514..10589)
                     /locus_tag="PKM80_01555"
     tRNA            complement(10514..10589)
                     /locus_tag="PKM80_01555"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10554..10556),aa:Phe,seq:gaa)
BASE COUNT         3734 a         1988 c         1970 g         3608 t
ORIGIN      
        1 aggcgcatcc atcaagaaac gtgaggaaac ccacagaatg gctgaagcaa acaaagcttt
       61 tgcccatctc agatggtaaa accaatcaac atcttaactc aataccccgg ctgaaaaagc
      121 tggggtattt gctttgaatc tgcaagtttg caattgacaa gataccctgt ttttaatctc
      181 tagtacttat cagcgcgtcc ctgtagctca gttggataga gtggcaccct ccgaaggtga
      241 aggtcaggcg ttcaaatcgc ctcagggacg ccacaaaaat gaacaagtta accaataaat
      301 gggattaaca ggttaacaga tgaaaaagtg atatcctaca tctttattat cggtattaca
      361 taaattatat gaatgaagta ttacttacct tggaaagtgt ttccttcctg tatggagata
      421 gaatcatcct gcaagatgta tcctttggca ttcacaattc agaaaagatt ggaattgtag
      481 gcattaatgg aagtggtaaa acaactcttt taaatatcat cggtggtatc attaagccca
      541 gtacagggat tgttacttca agaaaagatc taaaaattgg ttttttagaa cagaatcccc
      601 agtttattcc agagctatct gttttacaac acactatacc tacccaagga gaaataaaag
      661 atgaatatca ttataaatca atattaagcc gtttaggtat agaagattac aatcaaaaaa
      721 tgattttatt atcgggaggg cagcgtagaa aagccgattt ggcaagggtt ttagctttag
      781 aaccagattt attgatttta gatgaaccta ccaatcattt ggatttggat actatagaat
      841 ggttacaaaa ctatttagcg gatacgaata aagccattat ttttgttact cacgatcgct
      901 attttttgga tgcagtatcc accaaaatta tagaaattga gaggactaga ctattttatt
      961 acgagggaaa ctatagtgcc tacattcacg gaaagctaat tagagctaca gacagtcaac
     1021 gaaaagaaac acgccgtcag gcacagctta aaaaagaatt ggattggtta aaccggggtg
     1081 ccaaagcacg aaccagtaaa cctaaaaacc atatagatcg cgtaaaagag ctattaagca
     1141 aaagctacct tatttccaat caagatttgg atatatcttt tcaaatagat cgaatgggca
     1201 aaacgattct ggaattgcat cgcttaagca agtcctatgg aagtcgagtt cttttcgaag
     1261 atcttgatca taattttcaa aacagggaac gcattgggat tataggtccc aatggctgtg
     1321 gaaaaacaac tttactgaaa ataattgcag gagaagatga accggatatt ggtgttgtaa
     1381 aagctggtat aaacacccat tttgcttatt ataaacaaga tgaagatctc ttcgacccca
     1441 acactacagt ttatgaatac atcgctcaat ttgcagaagt gattaaaact gagaacggaa
     1501 ataaaattac tgccgcagaa atgcttaagc gttttctctt cgatggtaaa atgcaacaaa
     1561 tgaaattggg ttcattatcc ggaggtgaaa gaaagcgtct ctatttactc aaagcactga
     1621 tgtttggtgc aaatttcatt attatggatg aaccaaccaa cgatttggat attcgaactc
     1681 tggaaatttt agaggactat ctggatgcct ttaagggctg tctattaatt gtatcacacg
     1741 atcgtttctt cttggataga acagtggatt acctttttat ttttcaggaa ggaaagattc
     1801 gtaaatttgc cggtaattat tcagattatt tgctggtaaa gcgttttgag caagaagaag
     1861 atacaacgaa gaaaaagcct gctattttac gtcccaaacg aatttccaag ggtcttacct
     1921 ataatgaaca acgagaattt tctcaaaccg aaaaagatat tgagaagata gaaaatcagc
     1981 tcatagaatt ggaagataag ctaaatgatt tttcccagac tcttactccc aatgattatc
     2041 atcaaataag cataacaata cttaaattgg aagaacaaca tcaaaacctt cttaaccgct
     2101 ggttggaact gagtgataag caagacaact gatttaatat acaacctatc aaatatttcc
     2161 ttaaaaaaag gaggtacaaa atgatatcag aattagtcta ccgcaatcgt agttatcgta
     2221 ggttttacga atcaaaaagg ataactcact ctcagcttat tcagattatg gaaacggcaa
     2281 gatttattcc cagcgcaggt aacttgcaga aattatgtta ttatctgatt gaagagcgta
     2341 gtgaatgtga taaaatgttt ccacatttga aatgggcaag ttacttgaaa aaatggaatg
     2401 gacccgaacc aggagaaaga ccttcagctt atataattat tctggttccc aaaagctgtt
     2461 ccaatatggt ttatatcgat accggtatag cagctcagac aatactctta accgctgttg
     2521 ataatggctt gggaggatgt ttgttggctt ctgtcgataa agatgctgtt cataaaatat
     2581 tcttgctgcc cgataatatg gatatcgtat tagttatagc tcttggttat ccagctgaaa
     2641 cagttgtaat agaagatgtt acagaccagg ataagattga atactggcgc gatgaaaata
     2701 ctgttcatca tgttcccaaa cgcaaattga gtgatcttat tctaaattac tgatcgtata
     2761 aagttgccag gtattctagt tataattgac aaatatagat agtttgggat agtggtttag
     2821 ttatggaaaa tgtaattgta tacagcgttt atgaagttac caggcagatt cgtcaggtta
     2881 ttgaatcttc aattgaacct ctgtatgtga tgggtgaaat atcaggtttt gttcatcata
     2941 gttcagggca tatatatttt aatctgaagg acgaaaattc ctccctacgc tgcacttttt
     3001 tcaaaggggc caattataat ctaactttca aaccggaaga tgggatgcag gtaatctgtt
     3061 atggtaaatt gaccgtttat gaaaaggggg gaacttataa cctgaatgtt ttaaaaatgc
     3121 aagaagctgg acaaggactt ttacaacaga aatttgaaat attgaaaaag aagctggaaa
     3181 aggaaggtct ttttgcaaca gcacataaaa aaccaattcc taaatatcct gagcgcattg
     3241 gaattgttac ttctccaacg ggagcagctc ttcaggatat attaaacatt atcagccgtc
     3301 gctatcctgt tgagataatt gtttatcctg cattagttca aggtttggaa gcccctcccc
     3361 aaattattaa tggtataaaa tacttcaata caactaaaga tgtggatttt atcattctta
     3421 ccaggggggg cggttcgcaa gaagatctat tttgtttcaa tgatgaatca ttagttagag
     3481 ctatattcga ttccgattta cctataattt ctgctgtagg acatgagatt gattttactt
     3541 tggcagattt cgttgctgat ttacgagctc caactccttc agcagcagca gaattagcag
     3601 ttcctgataa gaaagacctg ctaaacctgt taaattcttt aaagaaaaga ttggacttaa
     3661 cggttcaaaa caagctggaa atgaaacata aaccaattat gaattatcaa ctacagctaa
     3721 cccaatacca tcccgaaaag gtatggcgag aatatcagca acgttttgat ctggcagaaa
     3781 tggctatgaa aaatgcacta aaacaacttc cagataagcg tttacagttt gagatgagaa
     3841 cccaaaccat tatgcagcag gtaacaagca agattgatct gtgtatagcc caagctaaag
     3901 cgaacagtaa tttagcctgg catcaattaa atgacaagac actaaattta cttcacaata
     3961 aagccaatca cttaaactct ttaggcaaca ttctggagca actttctcct cgcagcattc
     4021 aaagaaaggg ttggataatg gcttttaaag atggaaagct aattagttcg gttaaacaaa
     4081 ttaccaaagg caatcatctt aatctcgatt ttattgatgg tatagcagat tccataatag
     4141 aaagggtgga aaataaagag tgaaaagagg attattgata ttttcattac tgtttctgct
     4201 aaagttgagt gcagaaattc gttcagtttg ggttttacct tgggatataa cgaccgaaag
     4261 cgctatagat caagttatta acacagccgt tgattgtaac caaaatgagt tgctagtaga
     4321 agttcgttac cgggctgatg cattatttga tacttcccaa ggtgcctatc tatatcccaa
     4381 tcctgaacct gtaagctata tattgaataa ttcatcattt gatccgttaa cttatataat
     4441 ccaacaagca cataataacg gcttagcagt tcaggcttgg attgtagttt tcaatgctac
     4501 tcaaactgat cactatctta ttcgacagaa ttatatctat aacaatcatc gcaattggat
     4561 cacttacgat aaaaacggtt accaaatgaa taatggatac cgttatggtt attttatcga
     4621 tcctggaatt cctgaagttc aggaatattt gctaaatatt gttggaaata tagcagctgg
     4681 ttatcctgaa ttagatggta ttcatttaga ttatatccgt tatccggatg cggatttagg
     4741 ttatcatcct atttcccaag aactttatag agcatattgc ctaaatcaga tggatatcac
     4801 ttataatgaa tggcggataa tgcaaatcaa taattttgta gaaaaggctt accacctaat
     4861 caaagaaatc aatccttctt tattattaac cgctgccgtt tttgctgata ttgccgatgc
     4921 aaatgtatct tatgctcaag attggcagtc ctggcttaag aacggtatta tcgatagggt
     4981 ttatcccatg gcttatgata ttaattatgc aaaattcagc aaacacacag aacaaatgaa
     5041 actgttaaat tttgattcaa aaatagtgat cggtttacgt gcctggaatg aaaatggttc
     5101 ttctcttgcc cttggtaaag gagcttcata taatgttcag aatgtagcta gaaagataga
     5161 tttagccaga gagcaaggat ttgcaggcat atctctattc agttattcag gtttaataaa
     5221 aggaaatgcc tgggcgcagc ttaccaggct aagttattct gagaaagcag aatatccttt
     5281 tgaaaaagtc agtactccac tggttaatca atctgttaca gaattaaaac aacctccgga
     5341 tgtaaagctt ttacgattaa acaatgaaat tcttatacag cttcaaatac caagtgaggg
     5401 attatggaat tgggaacttt ataaagagag ttttttgcta cagcataata gatattatct
     5461 gaaagggaat aacattgata tcttgaatgt tataaaccct aatatttgtg ctagtatcga
     5521 accgggacag tatatagttc aactctatca agaaaattct tccttcaaat attttattcc
     5581 agtagaactg aaaatagcca atgagtgatc ttccaaaaca ggcaggtatt ctttccgcta
     5641 ctgaattctt ccgtttagca atcaaatcta taattggaat tgctttagcc cgcataatta
     5701 ctccagcaga attaggtagt taccgtcaac tttttttgat ttatactact ttctctactc
     5761 ttttattgtt aggaattccg caaagtatgc tatattttct tcccaaagca gaaaatgcgg
     5821 aaaagcaaag gttattgata acccgcagtt tgaacctgat ttctatttta gcactcattt
     5881 ttgccttgtg tttgcttatt ttccggaatt ttattgctca aatatttcat aatccccaat
     5941 tgggaaatct gctaattatc tatgctctat acccgttatt tctattcgta acacaattat
     6001 atagctctat aatgatgggt ttgaaacaac ccttgagagc agcaaaattc actatctttt
     6061 ctgtgctttc ggattttatc tttattgtaa gtgccgcttt ttttacccgc aatcttacta
     6121 ttataatctg ggcagtgatt gcttctgctt ttctgcaatg gctttatgcc caaattactc
     6181 tcaacgaatt caaagaaaaa aaagttcgct ttgattttgc ctcagttaaa gaacaaatga
     6241 attatgcttt gccacttggt ttatcctcta ttattggaat gctcagtatc caattagata
     6301 agttaatgat atccggtttt ttcccgccgg aaaagtttgc tgttttctct ttaggagcaa
     6361 cggaatttcc gattgttggc atattagcta attccatcaa ttcagttttg ttaccgcatt
     6421 tgagttctac caataccgca actatgggag aactttatag tggtgctgtt cgaaaaaatg
     6481 ccattctggt ttttccttta atggctttat gttttatttt tgctgaaccg atctttatat
     6541 tattatacgg taaaatttat gccgaagcgg ctgtttattt ccgcatctat ctgtttttaa
     6601 tgcctttacg gattgctact tatggcattt tgtttcaagc attcgggaaa accaaattca
     6661 taatgtataa ttctctcttt gtgcttattg ccaatgctgt gttaaattat atcttaatca
     6721 tcaatttagg aatgaaaggt gcagcaattg caacagttat tgtcacttgg ctatcggtta
     6781 tgatttacct tatccagatt gctaaggtct taaaattaaa gttgctggac tttttcccaa
     6841 ttggaaaaat aacaaaaaat gcccttgtaa ccattatttc cctctttctc agttttctga
     6901 taatatggct tgggaaacaa aatcttctct ttgtagccat cggaggaatt gtgttcattt
     6961 tgagctattt actattgggt agaaaaaccg gagttatttt ggattatgat gttcaacttg
     7021 ccaaagaact tatcaatgat ctgaaaggga agttcaaaaa atgaattatt atgctgaatc
     7081 cacctggaaa aattacgatc ctgaacagca agcagatata aggattgcca agcttgtatc
     7141 actttttccc gaaaatatgc attattataa atataccaat tgtggcttaa catacaatgc
     7201 ttatgggcat ttacaatcct tttctaaaaa tactttgaat tctcttttaa ctctggaatt
     7261 ttcttttaca gcccaagata tactctgtcc tttgaatagg gattttattc cctttttact
     7321 tgatattaaa cagcattggc taaaacaatg gtttaatcct ccttcaggaa ctatcgattc
     7381 caactgtgga aaaagtgaat tcttaaataa aaaatctctg ttcacgaagt ccattaatta
     7441 ctttattaat atgtcaacga agccacagcc ctattggttt atggaattgt ttgtctggcg
     7501 ggagaattga aaatgaaaaa tttactgttt attacttatc attttcctcc tacaggcggt
     7561 tccggagtcc aaagaggact taaatttgcc aaatatttac cgcaattcgg ttttagacca
     7621 atagttcttt gtgccgatta tcgctgctta aaacaaccca aggactatac tttgctaaaa
     7681 gaattacccc ccagtgtaaa aatttatcgc accttcactt tggatgcgaa ctggtttttt
     7741 aagctgctgt ggggattaaa attaaataaa gtagtcacct tcttgcaacg gtatatcttg
     7801 attccagata cagaagttct gtggctacct tttgctttct taaaattaag aaaaattctt
     7861 gatgctaatc atattgattt agttttaatt tctgctccac cctattcctc gctatttttg
     7921 gctaaatatt gcaaacatcg ttaccagcta aaggtctgtg tggattttag agacccttgg
     7981 agttttggga taggcagaaa atacttaaaa ccacccagct ggatagcaaa taaagaaaac
     8041 aattgggaaa aagagattgt aactttggca gataaaatta tctgtgtaaa cgacaaaatg
     8101 gttgatgaat tcaggcaact ttatactaaa attccagccg ttaaattttg cagcatcacc
     8161 aatggctatg acgaagagga cttttctata tccactcccc taaaaaaaag cgataagttc
     8221 aaaattgtat atactggtag tttttatgat gccagaagac cgttaattct ctggcaggca
     8281 ttatgggaac tgatagaaga gggaaaaata aacccgcaaa aaatcaccgt gaatatttac
     8341 ggtagaaata ctaaaaattt cgttttaggc gaatatcagg ataatctcct tatccaaaag
     8401 attgtgcatt tctacggcta tactccgcat acacaaataa taaaaattct cttatctgcc
     8461 gatgtgcttt tattatattc tggttttggc aatactgagc agctgaatag tccagccaaa
     8521 ttatttgaat atttacgcag tgggaaaccc gtttttgcca ttatagatcc agaaagttcc
     8581 gcggctgaaa tacttctacc agcaaatact atttttcttg ctaattccgc ttctttaagt
     8641 tccataaaaa atacattatc agaactatat actcaatggg aagaaggcaa attgaaagtt
     8701 agcccagatt gggaatatat caaaaatttt gaacgcaaag ccttaaccgc taggcttgca
     8761 gaagtactat cttctataac ataatgaaaa tcctcattat cccttcttgg tatccatatc
     8821 cgaaaaaacc ttacgctgga aaattcttta tagatcaggc aaaggcatta tctgagaaca
     8881 gcaactggca atttgaaata ttaaactggg gacagaatga attccaaatg caaattaggt
     8941 atcctctatc ttcaattgca aaattattcg cttatttgaa agcaaaacct tcctctcgta
     9001 atttcaattc taacttaaaa gagctattca tcccacacat cacctggact agtaaactgc
     9061 tatcgggaaa cataaagacc ctaataaaga aaatggaatt aattcccaaa ccggatataa
     9121 ttcattccca tgtaacattt ccagctggct ttgtagctat gcttttcgca gaaaaatgga
     9181 aaatcccata tattatcaca gaacatagcg gtccctttcc ctttcctgaa tttgtttcta
     9241 ataacagaat ctcggaaaaa attactcttc ctcttcaaaa agcagcagca gttatatctg
     9301 taagtagata tcttcaaaaa cagattctat ctacctgttt ggtaaaaagt atagttatcc
     9361 ccaatttgat tgatacagaa ttctataaac cagtttctat atcagaaaaa agaagacgct
     9421 tttccttctt ttctttaagt gcactaattc ctgccaaagg tgttttagat ttaattgaag
     9481 ctttccgact agcagtaaat aaaggtatgg atgctgatct atatttaggt ggatctggtt
     9541 atttagcacc caaattaaga aagctgatta ttcaatacca tttggaaaat aggattcatc
     9601 tactaggata tcttacacct cagcaagcgc ttgaacaatt taataaatgt aattgttata
     9661 tcttaccaag ccatatagaa tctttcagta tggttttact ggaggcaatg gcttgcggga
     9721 aaccaattat agctacagat tgtggtggac caaaagatat aattactgaa acaacaggag
     9781 tccttatccc tcctcaaaaa catgatgtta tggctataga aatgctaaaa atggccaata
     9841 acagcaacaa atataaccct gatagcatca gagaatggtg tttaaaaaat tattctcctc
     9901 atataatctg tgctcaaata tcaagagtat atgaaagtat tactggaagt taaataatct
     9961 tctaactgtt taggactttt tcaataacct caatcatctc attgcgcata aatggctttt
    10021 ccaatactgc tttagctcca agttttaatg ccagaggaag atagctgtat ggaccgtatt
    10081 ttcccccacc actcatcgcg atgattttga ttccaggttt tatggccatt aagtcctgaa
    10141 tggtctccaa accttcttta ttaggcataa tgatgtccgt tataactata tcaagatcaa
    10201 attcacagca tatttctaat gcctgatcgc catcacctgc ctgctgaaca aagtatccag
    10261 aattttttag catttgtact aaaacattgc ggaaacttac atcatcttca acaactaaaa
    10321 ttttagtcat taaatatcct cgtttatgta atatatattt atagtaatta gttaattaaa
    10381 actaggttgg aaccttaatt tctatcccgt ttcattgtaa aggagaattc tttagataaa
    10441 tagaattttg aaataaaaat gtagaatatt atccacaatg cgtaatctaa aagggttttc
    10501 aagtgcaaaa aaatggtggc gaaacccaga atcgaactgg ggacacaagg cttttcaggc
    10561 ctctgctcta ccgtctgagc tatctcgcca accattttcc tctttaattt taccagcttt
    10621 ttctgtcaag aggaaattat gttgactttc tatcttattg ttttgtaata tgttaacgaa
    10681 atgttctttc cctcttggat agctctatat taaattcaaa ttgattgata agcattattt
    10741 gggcatagat aagactattc tttcaaactt tagaaactcg taaaatacta taccattgct
    10801 ttataattct ataatttcgt tagaggatcg ttaaagaatc taataacaaa tgaactatta
    10861 tctgctgcaa agtaaaaata ctatataaaa ccgttgaact attatgagtt aaggcaaaat
    10921 tctgataatc tatgacgaaa ttaggagaga tatttgaaaa acttcaatgt ttttttctgc
    10981 tgcttgattt ttagtttgta cacaaaaaaa cctgaatgcc aaggtttcta cctcttcaag
    11041 ctatggaaca gaatgaacca ggcagattag catattctcc tcaatatcat tgacgggttt
    11101 tacttctatg cactaaataa tagttttatc tataatggag agtggagaaa cggcatctta
    11161 caaaataaac ggttactttt ctcattaaat catagctctt accgacaaga gaagattatc
    11221 ttcaaccttg catcatatag tatatcactc attatactag ccttaatcat gcgttaatta
    11281 ttaaggagtg attaagggat
//
LOCUS       DAORHB010000026        11214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_4997_length_11214_cov_5.380948, whole genome shotgun sequence.
ACCESSION   DAORHB010000026 DAORHB010000000
VERSION     DAORHB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4997_length_11214_cov_5.380948"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(604..3402)
                     /locus_tag="PKM80_01560"
     CDS             complement(604..3402)
                     /locus_tag="PKM80_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNV92623.1"
                     /translation="MKKIVLSLILIGCLFSLGAILPPEYSALPLQQDPEVITGILDNG
                     LKYYIKQNAKPEKRIELRLCIDAGSVDEDDDQRGLAHFVEHMAFNGSKNFPRTEMVDY
                     LTSIGMGFHNGLNGGTSYNSTVYQFKLPTDDEAKLLKGISILSDIAWQLSFEPSEIER
                     ERGIIQEEWRLGQSAQQRIQDQIDKVRFAGSRYAERNPIGTIENLKSFKHNSLIRFYQ
                     DWYRPDLETVIIVGDCDPKKMENVIKEYFGAIPKRENPRPKVHYSVPDNIEPRAITVL
                     DKELPYTMLRSTWKVKSEPITELGSFYYSIKQDLFFTLVNARLQELSHQSNPPFSFAY
                     IYNQNWLKGFVATDCIMFANEGQSEEAFRTLITELARVRQHGFQPGELDRAKQIMIRE
                     AEQRVAEKKTMNSEDLVWEILDDANSGNTILSFEQYEQLLKGLINEIALSEVNEMVDE
                     VITSENLTLSLAGTAKEGVKYPSKDDLLNIYHQATAQELEPWEDITVNEPLLETIPNP
                     GKITKEKVYPKSEIKQWVLSNGIQVYSKKTDFKADEVIMIAQSPGGKAKLKPENYQAA
                     DLLNQYFYVAGFGNFDGNALKKAMAGKIADVYPTISNYSEGWRGSCSPQDMELMFQML
                     YQYNYAPRYNEAAFSSTVASMKTWTQNYLLDPSTAFFDTLDALIYENHPIKRQLHPED
                     LDTLTLKQIEDIYKDRFSDYSDFTFYIVGNFEEEQLKNYCRTYLANLPAKGRIEKRTD
                     VGVRTFTGKKDIVFTKGSDRSFVSNVTVGKTSVSPQTKVNQNALMILANDKLRENVRE
                     NMSGVYAVTVWSSYDFLPKLSFVTQTWMGCDQIRAKDLNAATFATLDSLKNGLFADKY
                     LDATRTTLHKTYEENINTNKYWINNMSNNVSLGLPIDSFMDYPALYDNVNKKAIMEAA
                     KQYFGFDKSCLSVYMYPE"
     gene            complement(3421..4032)
                     /locus_tag="PKM80_01565"
     CDS             complement(3421..4032)
                     /locus_tag="PKM80_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92624.1"
                     /translation="MKNRIWIILSFAIILLMLSACESGAKLKVYNRCSYPAYIKVEGQ
                     EFKTIGADEEVEYNIDTKTQYIFAGDVKKKLLIWLKGETYSIHDKDLNAYLDSTWIEV
                     QAGEIYKIFLLPNRASVKIVNASEQTISRAEIWQHSDLTHLKVGVLTNIMPGENEFLC
                     VEYGTDYYYQVEIYLSDGTHQTFGSYNTILDKDEQFLITYRSE"
     gene            complement(5110..5397)
                     /locus_tag="PKM80_01570"
     CDS             complement(5110..5397)
                     /locus_tag="PKM80_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92625.1"
                     /translation="MSKYKLKGNDLYDPYSHRIATIKGANIYDEHIHKVATIKGNDIY
                     NEHGNKVATLRGLDICDGHSHKIATLNDVHKAIDGAMGGASIVGLWLFFVR"
     gene            complement(5827..7977)
                     /locus_tag="PKM80_01575"
     CDS             complement(5827..7977)
                     /locus_tag="PKM80_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV92626.1"
                     /translation="MQHLRFLSVFFILLGFVSILESTKVSGIVTDVDKKPLESVRLVS
                     GKKTTLTRQNGSFTIEVMDSMQVSRLGYKKQTLSLKEISALPKDNRGIQIILQDEPIQ
                     LPAYYVFAYLGEENISAADVVTLPIDPDKNYNSASELISQTSSFQSTGTQLKGEIAEI
                     NILGNISRHTLVLVDGIAMNTLGEPFDFSRLNSENIASIDVVKNNASVYGGGSAIGGI
                     IMITTKQGAAIKTNLESKTEFGSYGYLMQQITVSGLNPKNSYRLCLNAYNTDNDFPIR
                     KGELIPEDSESIRKYNSKQQLSFSGAYSTRIAKMLTSFSTDYLTFQRELPGPLNFADL
                     YYKSFLEGSSLRPQIRLSANFGNFTWQSNNWLLKDETTYDNTQSLIEGFSQKYKQKMD
                     NYGFRQGLNYNSQFFQAAFSGEYSYNRYRYQDLINPHQSKIDYVRNQFALALKLNQQK
                     EFAWLIVNNGLAGRYDYIEGEDEFSGRLEFSVRNWGNNYLETGATIGNSFALPSPFDL
                     YWKGDSQTLGNPNLKSEKATGYQIWIKGNIPQATLKATLHQNKIKNLIQWRQVQMYGP
                     VWKPMNIGKATVRNLELEGNCQPLKQLELTASAVFTKAKDTTYYSFEDAPKLMYRPEV
                     LYSFALDWQPGIFHFWTKYSFTGKQWITPDNLIDPISAYALMDCGLSLHWQKDNWKFT
                     PALTIKNLTDENYMVHAYVPEPGRTFYATLQLNR"
     gene            complement(7989..8756)
                     /locus_tag="PKM80_01580"
     CDS             complement(7989..8756)
                     /locus_tag="PKM80_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92627.1"
                     /translation="MIELKNVTCGYGDFPVLKDISLQIAEGDFCALLGPNGAGKSTLL
                     YTIMGYLKPSEGYITIFEKDIAKIKHSWLAKQIAFVPQETRSEFDHTVQETVLMGRYP
                     YLGFLQSYSSEDYEAVDAVLENLKLTEFKHRWLSEISGGEKQRVLIARALVQQTPFIL
                     LDESLSQLDINYQIEIMKLLRTIHSKENKTVLIVSHNINLASNYAERLIFLKEGKLLA
                     AGKPEDLMQKETLKDLFAVELDIMINPHTGHPNIIYP"
     gene            8907..9122
                     /locus_tag="PKM80_01585"
     CDS             8907..9122
                     /locus_tag="PKM80_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92628.1"
                     /translation="MIIVLAIYPLNKTDYLLKVKRGILFNLGKEEDLFICTLSKKPGV
                     CLFYFFLSLCKRLSSKKAVDIFSLSGG"
     gene            9157..10005
                     /locus_tag="PKM80_01590"
     CDS             9157..10005
                     /locus_tag="PKM80_01590"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV92629.1"
                     /translation="MRENPKSIFTIDVEDYYNVTEERGEPPISEWDFLPSIVESGFMK
                     LLNLLDLYQVKATCFFLGYVAQKYPHLVIEAKKRGHEIASHGMFHRLVYKLSREEFIA
                     DLLSSKKILEDIISEEVISFRSPSFSVTESIPWFFDALVEAGFKIDSSVFPVKRDYGG
                     YQTTQKKPYWIKTDKGMLCEFPISVAPFCGKDFCFFGGGYLRLFPKRIILSMHSKLAR
                     EGNPTLFYIHPREMEPQHPRLKMNKLAYFKSYVNMNTVKPKLEAILSSSKFITCKEYM
                     AIIKDN"
     gene            10006..10650
                     /locus_tag="PKM80_01595"
     CDS             10006..10650
                     /locus_tag="PKM80_01595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV92630.1"
                     /translation="MQNNKKTASFFDGYSGDFNAIYGTGTGAFQRLINKWFRQTMLIR
                     FQKTIDGCKPLEGKTILDIGCGPGIYDVTLAKNSPAYVYGIDFAPGMIELAKNEAKKA
                     GVDKICEFEIADFNTLPEDKKYNYIILMGFMDYMENPKQVIEKAMRLATDKVFFSFPS
                     NKGFLAWQRQIRYKFKCPLYLYSEKQLQELFSNIAPWKSRIENIGRDFFVTLYK"
BASE COUNT         3447 a         2126 c         1925 g         3716 t
ORIGIN      
        1 tcaaaacaat tttgaggcta acactatcct ctggaatgga atagataata aaggtatgcg
       61 cagcgcttcc ggaatctatt tcttgaaagt gaaagttact ggcgacaaac ctgtaacccg
      121 taaaatattg gttctataaa ttagttccga actttcagct ataccagatt cgggataata
      181 catcgctggt tattgctgaa atagcaggaa ttcaccaaag ctatatacgg tgaatctata
      241 tttgaagagt gaaaatagca taatataagg gtggtagatg ttacatctgc cacccttttc
      301 ttattcatcg ctttatcttt tttcccttat ccttgcttag ttagctgtgc aggtactgca
      361 atggtgactg ttgggatttt gccgagtagc tccacgacag ttttaccaca actaattaac
      421 tagggactgc agacagtaaa aacaaaatga gaattgttct caccgggtta aaacccattg
      481 ctcaaatcag taggtctgct gggactttta cttattaaat tatcccataa atagaatctg
      541 tgttatctgc aagaaacatc tttttcttag ttatctgcgg gaaacctatc tatgagctac
      601 tttttattca ggatacatat acacagaaag gcaggattta tcaaagccga aatattgttt
      661 tgcagcttcc ataatagcct tcttatttac attatcataa agagcaggat aatccataaa
      721 gctatcaatc ggcaagccca gagacacatt attgctcata ttattgatcc agtatttatt
      781 ggtattgatg ttttcctcgt aggttttatg cagagtagtt cttgtggcat cgagatattt
      841 atcggcaaaa agtccatttt tcaagctatc cagggtagca aaggtagcag cattcaaatc
      901 tttggctctt atttgatcgc aacccatcca agtttgagta acaaaactca gtttaggaag
      961 aaaatcataa ctactccaga cggtaacggc ataaactccg ctcatatttt cgcgaacatt
     1021 ttcacgcaat ttatcgttag ccagtatcat caacgcattt tgattaacct tggtttgagg
     1081 ggaaacagat gtcttaccaa ctgtaacatt ggaaacaaag ctacggtctg atcccttggt
     1141 aaaaacaata tcctttttcc ctgtaaaagt tcttacgcca acatcagttc ttttttctat
     1201 tctacctttt gctggtaaat tggctaaata tgttctacaa tagtttttta gctgctcttc
     1261 ctcaaaatta cccactatat aaaaggtgaa atcggagtaa tcgctaaagc ggtctttata
     1321 gatatcttca atttgcttca gagttaatgt atccagatct tccggatgta actgtctttt
     1381 tataggatgg ttttcataga taagagcatc caaagtatca aaaaaagcag tactgggatc
     1441 cagaagataa ttttgcgtcc aggttttcat tgaagctacg gtagaagaaa atgctgcttc
     1501 attataacga ggggcataat tatattgata cagcatctga aacattagtt ccatatcctg
     1561 aggtgaacag ctacctctcc aaccttctga ataattggat atagttggat agacatcagc
     1621 aattttgcca gccatagctt tcttcaaggc atttccatca aaattgccaa aaccagctac
     1681 ataaaaatat tgatttagaa gatcagctgc ttggtaattt tccggtttca gtttggcttt
     1741 gccaccaggg ctctgagcta tcattattac ttcatctgcc ttaaaatctg tctttttact
     1801 gtaaacctgt ataccattgg aaaggaccca ctgtttaatt tcggatttgg gataaacctt
     1861 ttctttggtt attttacctg gattaggaat ggtttctaaa aggggttcat taactgttat
     1921 atcttcccag ggttccaatt cttgagcagt tgcctgatga taaatattca gtaaatcatc
     1981 cttacttgga tattttaccc cttcttttgc tgttcctgct aagcttaaag tgagattctc
     2041 ggaagtaatg acttcatcaa ccatttcatt tacttcgctt aaggcaatct cattaatcaa
     2101 acctttcagt aattgttcat attgttcaaa gctaagaatt gtatttccag agtttgcatc
     2161 atccaaaatt tcccaaacca gatcttccga attcattgtc tttttctccg caactctttg
     2221 ttccgcttct ctaatcatta tttgtttggc tcgatctaat tctccaggtt gaaaaccgtg
     2281 ctgacgaacc cgagctaatt ctgtgatcaa ggttctaaag gcttcttcac tttgcccctc
     2341 attagcaaac attatacaat cggtggcaac aaaacctttc agccagttct gattatatat
     2401 ataagcaaaa gaaaaaggag gattagattg atggcttagc tcttgtaaac gcgcatttac
     2461 caaagtgaaa aataaatcct gctttatact ataataaaag gaacctaatt ctgtaatagg
     2521 ttcagatttt actttccaag tacttctaag catcgtgtat ggcagttctt tatctaaaac
     2581 cgttatagct cgcggttcta tattgtctgg aactgaataa tgaactttgg ggcgaggatt
     2641 ttcccgcttg gggatagcgc caaaatattc tttaattacg ttttccattt tcttgggatc
     2701 acaatcgcct acgatgataa ctgtttctaa atcgggacga taccaatcct gataaaaccg
     2761 aattaagcta ttatgcttga aacttttcaa gttttcaatt gttccgatgg ggttacgctc
     2821 agcataacga gaacctgcaa aacgcacttt atctatctga tcctgaattc gttgttgcgc
     2881 gctttgtccc aatctccatt cttcttgaat aattcctctt tcacgctcaa tttcactggg
     2941 ctcaaagctt aactgccagg caatatctga taaaatggat atccctttta acagtttagc
     3001 ctcatcatcg gtggggagtt taaattggta aactgtggag ttataacttg ttccaccatt
     3061 taacccattg tgaaaaccca ttccaataga agttaaataa tctaccattt cagttctggg
     3121 aaaattcttg ctaccattaa aagccatatg ctctacaaaa tgagccagac ctctttgatc
     3181 atcatcttca tcaacactgc cagcatcaat acaaagccga agttcaattc gcttttcagg
     3241 tttggcattt tgcttgatgt aatattttaa gccattatcc aatattccag ttattacttc
     3301 cggatcctgc tgcaagggta aagctgaata ttcaggagga aggattgccc ctaaagaaaa
     3361 gaggcatcca atcaggatca agctgaggac tatttttttc atttgtactc cctgttaatt
     3421 ttactctgat ctatatgtaa ttaaaaactg ttcatctttg tctaaaatgg tattgtagct
     3481 tccaaaggtc tgatgagttc catcggaaag ataaatctcc acttgataat aataatccgt
     3541 tccatattct acgcataaaa attcgttttc accaggcata atattcgtca atacccctac
     3601 ttttaaatgg gttaaatcac tatgctgcca aatttctgcc cgactaatag tttgctccga
     3661 tgcatttact attttcacgc tggctctatt gggtaaaagg aaaattttat aaatctcccc
     3721 tgcctgcact tcaatccagg tagaatcaag ataagcatta agatccttat catgaatact
     3781 ataggtttct cctttcagcc aaattaacag cttctttttt acatcaccgg caaatatata
     3841 ttgagttttg gtatctatat tatattcaac ttcttcatct gcgccaatag ttttgaattc
     3901 ttgcccttca acttttatat aagctggata ggaacaacga ttataaactt ttagctttgc
     3961 ccctgattca caagcggaaa gcattaaaag aataatcgcg aagctaagga taatccatat
     4021 tctatttttc attattcttt cctcccttct taaaagttat cccttttatc agactctaac
     4081 tgtcaagtaa attgttggtt gaccagatcc acactagttt tttataatag gtaggattaa
     4141 ttgctgatat ctcttccctc tttgaaagcc cttttgggca atacaaaaat agcgaagtga
     4201 cgtaagccat tttcaaaaat tcacaatgca gacggcaaaa ggttttggct tacaattttc
     4261 acctacatta cccaaaacca cattccacga actttttact aacaaccaag ctataactgg
     4321 tttccgaatt gtaaaattgt gagttaaaac agagtatttt tctaaaagat ttttcctaaa
     4381 agagtggatg ctaaagagat aaatatatcc ctaaggtatt cattcctata tgataactat
     4441 aagtgtcaat tacaattgga aagattaaag taacaactta aagatattgc gatcatttcc
     4501 ctattgagac agtattatac cgatttttgt aatcacttat ataataaaaa cctactattg
     4561 ttgtctctta tctcccttga ttaagattag ataaatacct gactaacgct ttgtattaag
     4621 aacgaagagc ggtgactgaa tgtaatatgc agtttatact aaagtagtaa atacctgctg
     4681 acaggtaaat atattaggat aagacataat accaacacca aattttaaag ttcgggagaa
     4741 aatgagctaa tcaattctta ctttacaaat aaaccgaagt ttgccaacca acttaatgct
     4801 tacctgtttt ttagaagttt tttattcggc acctcatttg ctgactatgt ccataaactg
     4861 tgtggcttac ttattcggat ttttttgcca gtgggaaatt cctgatgaat tgtagaaacg
     4921 gtatattcct tcttccttag taactgaact tattaaaaag ggggatactt tttcccttta
     4981 cttccatact atgcaagcaa catttcttgt caaaaatgaa atttgtagtg ccaaagacaa
     5041 tattttaatt tttatctatt tctaattcct ctcatttaca aaattgaact gtcaaacatc
     5101 agtaattggt taccttacaa aaaacagcca caaacctact atagatgcac cacccatagc
     5161 cccatcaatg gccttatgca catcatttag ggttgctatt ttgtggctat gtccatcaca
     5221 aatgtctaac cctcttagag ttgcaacctt attgccatgt tcattataga tgtcattacc
     5281 tttaatagtt gctactttgt gaatgtgctc atcataaata ttggcaccct taatagttgc
     5341 aatacgatga ctataagggt cataaaggtc attacctttt agcttgtatt ttgacataat
     5401 ggtctccttt actgtttttt tgctgagttc caaatgttag tatccatacc aacttaatgt
     5461 ttctcttatt agttgagggc atctaacatc ccttgttgct cacaatctat gtaaagttag
     5521 cgtctaattg catagttttc agtttgctaa tttaggtcaa gttctttttt gtaaaatttg
     5581 cattacccta aaattatctt agttactttc tcaaatatga taaactactc aaataattta
     5641 gggaaggaca aatatcgttt cgtttatggg ctggttataa ctaaaatggt gtgcgatggt
     5701 tagatgcgta aatctaattt agtttcccta aagctgtttc tgcaagaaaa agtcatctgc
     5761 taagttgcaa attggcataa aaaagttatt tattcagttt cacagtttca cagtggcata
     5821 aaaaagttat cggttcagtt gcaaagtggc ataaaaagtt ctacccggtt cagggacata
     5881 agcgtgaacc atatagtttt catccgtaag gtttttaata gtgagggcgg gggtaaattt
     5941 ccagttatct ttttgccaat gcaagcttaa tccgcaatcc attaaagcat aagcagaaat
     6001 aggatctatc aaattatcgg gagttatcca ttgttttccg gtgaagctat attttgtcca
     6061 aaagtgaaaa atccctggtt gccaatcaag ggcaaaagaa taaagaactt caggacggta
     6121 cataagtttt ggtgcatctt caaaactata ataagtagta tccttagctt tcgtaaaaac
     6181 cgccgaagca gtaagttcca attgtttaag cggttggcaa tttccttcca attccagatt
     6241 ccgaactgtt gcttttccga tattcattgg tttccaaaca ggaccataca tttgcacttg
     6301 ccgccattga atcagatttt taatcttgtt ttggtgtaag gttgctttta gcgttgcctg
     6361 gggaatattg ccttttatcc agatctgata accagttgcc ttttcacttt taagatttgg
     6421 attacccaaa gtttgagaat cccctttcca ataaagatca aaaggcgaag gcagagcaaa
     6481 agaattgcca attgtggctc ccgtttccag ataattattt ccccaattgc gaacggaaaa
     6541 ttccagccgt ccgctaaatt catcttcacc ctctatgtaa tcgtagcgac cagccaaacc
     6601 gttattcaca atcagccagg caaattcttt ttgctgatta agctttaagg caagcgcaaa
     6661 ttgattccta acataatcaa tcttgctttg atgaggattt attaaatcct gatagcgata
     6721 gcgattataa gaatattctc cgctaaaagc tgcctggaaa aattgggagt tgtagttaag
     6781 accttgtcga aaaccgtaat tatccatttt ttgtttatat ttttgggaaa atccctcaat
     6841 taaggactgc gtattatcgt aagttgtttc atccttcaag agccaattat tactttgcca
     6901 agtaaaattg ccgaaattgg cagagagccg aatttgcgga cgcagcgagg aaccctccaa
     6961 aaaagactta taatataaat cggcaaaatt tagcgggcca ggaagctctc tctgaaaggt
     7021 taaatagtca gtagaaaagc tagttagcat tttggcaata cgagttgaat acgcaccgga
     7081 aaaagaaagc tgttgtttac tgttatattt ccggatgctt tcactatctt ctggaatcaa
     7141 ttctcctttc cttatgggaa aatcgttatc cgtattgtag gcattaagac ataaccgata
     7201 agaatttttg ggatttaatc cgctaaccgt gatttgttgc attaaatagc cataagagcc
     7261 gaattctgtt ttgctttcca gatttgtttt tatggcagca ccctgttttg tagtaatcat
     7321 tattatacca ccaatggcag atccacctcc ataaacactg gcattatttt tcactacatc
     7381 tatgcttgct atgttttctg aattcaaccg ggaaaaatca aaaggctcgc ctaaagtatt
     7441 catcgcgatt ccatctacta aaaccaaagt gtgtctgctg atatttccca ggatgtttat
     7501 ttccgctatt tcacccttta gctgagttcc agtactttga aaagaagaag tttgtgagat
     7561 taattccgaa gcgctattat aatttttatc tggatctatt ggcaatgtta ccacatctgc
     7621 agcagaaata ttttcttcgc caagataggc aaaaacatag tatgcaggta gctgaatcgg
     7681 ttcatcttgc agaattattt ggattcctct attatcctta ggcagagcag atatttcttt
     7741 tagggatagg gtttgctttt tatagcccaa acggctaacc tgcatagaat ccattacttc
     7801 aattgtaaaa ctgccatttt gtcttgttaa agttgttttt ttcccgctaa ccaaacgaac
     7861 cgattccaaa ggttttttat ctacatctgt aacaatacca gatactttgg tactttccag
     7921 aatagataca aaaccaagaa gaataaagaa aacggataaa aaacgaaggt gctgcatagc
     7981 gtaaactctt atggataaat aatattggga tgtccggtat gcggattgat cataatatcc
     8041 agctccacag caaatagatc tttcagtgtc tctttttgca tcaagtcttc cggtttgccg
     8101 gcagccaata gttttccttc ttttagaaat attaaccttt cagcatagtt ggaagccagg
     8161 tttatattgt gagaaactat taaaaccgtc ttattttctt tactgtgaat ggttcttaag
     8221 agtttcatta tttctatctg ataatttata tccagttggg acaaactctc gtccaacaaa
     8281 ataaatggtg tctgctgaac taacgctctg gcaattaata ccctctgttt ttcgcctcca
     8341 ctgatttcag aaagccagcg gtgtttaaat tctgttaact tcaaattttc caaaacagca
     8401 tctaccgctt cataatcctc agagctatag gattgcaaaa aacccagata gggataacga
     8461 cccatcaaaa cagtttcctg aactgtatga tcaaactcag acctagtttc ttgaggtaca
     8521 aaggcaatct gttttgccaa ccaagaatgt tttattttag ctatatcctt ttcaaatatg
     8581 gtaatatatc cctcagatgg tttcaaatag cccataattg tgtatagtaa agtagatttt
     8641 ccagctccgt taggacccaa aagtgcacaa aaatcccctt ctgcaatttg cagagaaata
     8701 tctttcaaaa ccgggaaatc cccgtaaccg caagttacat tctttagctc tatcattgtt
     8761 ttgttttcca gtttttttct ccttaagtat gatcattttt ttgcttgcgg tttatctgtc
     8821 aactgaactt tcagcaaaga tatgtttgtt ttttggtaaa aaagtctttt tatatctcgg
     8881 caaccaaata agaactcgga taaaagatga ttattgtttt agccatttat ccactgaata
     8941 aaacagatta tttgcttaaa gtgaaaagag gaatattatt taatttggga aaagaagaag
     9001 atttgtttat atgtacacta agtaaaaaac ccggtgtttg tttattttat ttcttcttat
     9061 cgctatgtaa gcgtttaagt tctaagaaag cagttgacat tttttccctt tcaggaggat
     9121 aggatattac ttgttttata ggaaatctaa atagttatgc gagagaatcc gaaatctatt
     9181 ttcactattg atgtggaaga ttattataat gttaccgaag aaagaggtga gcccccaatt
     9241 tctgagtggg attttttacc cagcatagta gaaagcggtt ttatgaaatt gctaaacttg
     9301 cttgatcttt atcaagttaa ggcaacctgt ttctttttag gatatgtagc ccaaaaatat
     9361 cctcatttag tgatagaagc aaaaaaaaga ggtcacgaaa ttgcatctca tggaatgttt
     9421 catcgccttg tatataagct aagcagagag gaatttattg ctgatttgct atcctccaag
     9481 aaaatattag aggatattat ttccgaggaa gttatatctt tccggagccc aagtttttca
     9541 gttacagagt ctatcccctg gtttttcgat gctttagtag aagcaggttt taaaattgat
     9601 tcttccgttt ttccggttaa aagagattat ggtggttatc aaacaactca aaaaaaaccg
     9661 tattggatca aaaccgataa gggtatgctt tgtgaatttc ctatttcggt agcccccttt
     9721 tgtggtaagg acttttgttt ttttggggga ggatatttgc gtctgttccc aaaacggatt
     9781 atactttcta tgcacagtaa actggcaaga gaaggaaatc ctactttatt ctatattcac
     9841 ccgcgagaga tggaaccaca gcatcctcgc ttaaaaatga acaaactggc ctatttcaaa
     9901 agttatgtaa atatgaatac agtaaagccc aagctggaag caatactttc cagcagtaaa
     9961 tttatcactt gcaaagaata tatggcaata ataaaggata actaaatgca aaataacaaa
    10021 aaaacagctt ctttcttcga tggctattct ggcgatttta atgccattta cggaactgga
    10081 actggagctt ttcaacgcct tataaataaa tggttcagac aaacaatgct gatccgtttt
    10141 caaaagacaa tagatggatg taaaccctta gaaggaaaga caattttgga tataggttgt
    10201 ggacccggaa tttatgatgt aactctagct aaaaactctc ctgcttatgt gtatggcata
    10261 gattttgctc ccggaatgat tgaattggcc aaaaacgaag caaagaaagc cggtgtagat
    10321 aaaatatgtg aatttgaaat tgccgatttt aatactctgc ccgaagataa aaagtacaat
    10381 tacattattt tgatgggttt tatggactat atggaaaacc caaagcaggt tattgaaaaa
    10441 gcgatgcgtt tggcaaccga taaagttttc tttagttttc cttccaataa gggctttctg
    10501 gcctggcaac gccaaattcg ctataaattt aaatgcccgc tttatctata tagtgaaaaa
    10561 caactacaag agcttttttc taatattgct ccctggaaat cccggataga aaatatcggc
    10621 agggattttt tcgttactct gtataagtag caatagtgta cactatctcc ccaaaagtgt
    10681 agccggagga cgccgatcct ccgcaagaaa aaatccaaaa agcggagctt cagcgagctc
    10741 cgactaccca aaagtgtagc cagaggacgc cggtcccccg caagaaataa tccaagaatt
    10801 tattagccca ttttcctccg cacttaaaaa ttgcaggtta atatttagta atatcctcat
    10861 atatttactt gtcatctgat attttctaca ttaacgccaa ctacatatct aatgcaatca
    10921 ccactcttcg ttcttaagtg aaagcgttaa tcaagtgttt atcactcctt aatcaagcgt
    10981 taattattaa cgcttgatta aggtatgtta aacgatagat taagggcttc gcacatgaaa
    11041 tatagaaaga aatacggaga ttctattagg aaagtggtag ggaaagccag cataatatgg
    11101 ttccgatagc taaatttatt cgatatttct aatttagtgc tttcagttgt agttgatagt
    11161 tctatgtatc ttataggaat gataacttaa ggggagatac tttttacctt tacc
//
LOCUS       DAORHB010000027        10611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_5509_length_10611_cov_5.004547, whole genome shotgun sequence.
ACCESSION   DAORHB010000027 DAORHB010000000
VERSION     DAORHB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5509_length_10611_cov_5.004547"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..2384)
                     /locus_tag="PKM80_01600"
     CDS             complement(204..2384)
                     /locus_tag="PKM80_01600"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNV92631.1"
                     /translation="MKTRYWIIPLLLMLCLLMAAKKEKYGSIYGSITEAGKNTFVADL
                     TVQCLQKNTVIATAVSDQNGFYRFDKLKPGTYTIKINDPNFAVYENKKVKIKANKPAL
                     LNIVLVANPQKPVPEKKTETGKLSITVTDNNTPLQYADVAIYDNNKRLTSGQTDAQGK
                     AVILNIPAGTYSLKCSLIGYNTVEKSNITIEAGKTTTLTITMQKTGIIMESVKVNAVS
                     DMDVVPWQTLLSASMQNQYITPFNTEQYEAIKPNIFHSPLAEPLSTFSIDVDTGCYSN
                     IRRILQQGQLPEPKSIRIEELVNYFAYNYPKPEGEHPFSVYTEMSVCPWNQNRNLVLI
                     GLQAKKLDFSKAAPSNLVFLLDVSGSMDEPNKLPLVKASMKLLVDNLRDEDKIAIVVY
                     AGAAGEVLPSTSARQKKDIYDAIDRLEAGGSTAGGAGIELAYKISEENFIKGGNNRII
                     LCTDGDFNVGASSSAHLTQLIESKRNKGIYLTALGYGMGNYKDNTLELLADKGNGNYA
                     YIDDISEAKKVLVNEMASTLYAVAKDVKLQIEFNPAHIKAYRLIGYENRLLNKEDFKD
                     DTKDAGELGAGHCVTAVYEIIPTASKEPIPELDELKYQDIKISDEARKSPEALTVKLR
                     YKLPDSDTSIPFEVPVMNKTIHLENASENYLFACSVIGYGMLLQDSQYKAALNWDMVK
                     ELATKNIGKDTEGYRTEFLKLIDLAAKLQEKQIREENRDFNKEY"
     gene            2634..2709
                     /locus_tag="PKM80_01605"
     tRNA            2634..2709
                     /locus_tag="PKM80_01605"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2667..2669,aa:Lys,seq:ctt)
     gene            2855..4021
                     /gene="metK"
                     /locus_tag="PKM80_01610"
     CDS             2855..4021
                     /gene="metK"
                     /locus_tag="PKM80_01610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNV92632.1"
                     /translation="MTAALHALDKTDRNGYVFTSESVSEGHPDKVCDQISDAILDAYL
                     AADPHSKVACETLATENRIILSGEISSKATVDIESIVRKTIQEIGYTSPNKGFDYNCQ
                     INNYLHRQESALEENTGAGDQGLMFGYACSQTQNMMPLPIELAHKLLFNLATARKKGT
                     IKHLLPDSKSQVSMRYLGRKAVDLKCLVISTHHDILTEMEFEELKEQIQQLIIIPSIR
                     DIEDGCKGYFETKNCEIKINPLGPWQDGGPAFDTGLTGRKIIVDTYGGWAQHGGGAFS
                     GKDATKVDRSAAYMGRHIAKSIVASGLADECLIQFSFVIGEQKPISLMVDTFGSGKES
                     DAEIERIIAKNFDLTVSGIIDYLNLRKPIYFPTASYGHFGRDNFSWERVKKITR"
     gene            4058..4855
                     /gene="kdsA"
                     /locus_tag="PKM80_01615"
     CDS             4058..4855
                     /gene="kdsA"
                     /locus_tag="PKM80_01615"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNV92633.1"
                     /translation="MSKLYTDLANSQNFFLIAGPCVIEEERIMYSIAETLKELCTRKG
                     IPLIFKASYKKANRSSVDSFSGPGLEEGLQTLAKIKQDFELPVLTDVHECIEITSAAD
                     VCDILQIPAFLSRQTDLIKNAAATGKIINIKKGQFMAPEDMQFAAEKARSTGNEQILL
                     TERGTTFGYHNLVVDFRSFTIMSQFGYPVVYDVTHSLQRPSLSNISGGNPEYAFSMAK
                     AAIATGKVKGLFIETHPEPQKAKSDAASMLSLDKMESLLDGCLQLLN"
     gene            4868..5428
                     /locus_tag="PKM80_01620"
     CDS             4868..5428
                     /locus_tag="PKM80_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNV92634.1"
                     /translation="MYDKRALNRPNKRVVPWNEIKLLVFDCDGVLTDGRFVYGTNNLE
                     IKNFHGHDGLGFKLLQHTDIIVCFITGRSSEALERRCLDLNITYLFQNVANKKECLDN
                     LLKDLSLTYTNVAYMGDDWNDVPVMRKVAFSAAPADAQPGIIHLADWVSEFKGGHGAA
                     RDLINYVLIHKGIYEKTILSYLDSIG"
     gene            5364..5942
                     /gene="lptC"
                     /locus_tag="PKM80_01625"
     CDS             5364..5942
                     /gene="lptC"
                     /locus_tag="PKM80_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNV92635.1"
                     /translation="MSLFIKASMKKRFCPIWIASVSVVILLSSCQKVDLQSHTGVLAR
                     DLPDEISYKVQLTQLNNDHNEYILEAERIERFYDRRLLYAYKVTLTTFDKNNQISSVI
                     KADTTIVDDARNLIFANGNASLNSPNGTLSTPRMAWDRTVDEITAPLEVTVTRKGNVM
                     YGRNLRTNSKLSFVEMDAVSAEGYFKEEEINW"
     gene            5975..7015
                     /locus_tag="PKM80_01630"
     CDS             5975..7015
                     /locus_tag="PKM80_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNV92636.1"
                     /translation="MLFSLYAKGQADEKFRLIHSDKLFMTKAGEEQALELNGNVHFFY
                     GNTEFLCRNALILDTQKIARLSGNVIVKNDSLTLEADTLAYYRIPDLMNLGGRITATE
                     KTKTGIYRWIKSNFASYDKANDILTTWNRVNSFDYQENAHIQCGYAQWDRKKGYAILL
                     EEPFITSGTKDTLSVAAEKMEYFDQEHKLIATFNVKVERNEFHATSDFLIYFLNEEKA
                     IFTGEPKFFSNFADAKAMEFHLFLKDRSLMRAELIDSCHIDFAEEQFMEKKNKVDADL
                     VTMDFLDDQLRIFVAEGKVDYYFQQDQTEKKDFMINSATGAYLQANFDDNSKLQSLQM
                     KTDIKGKYVFQQ"
     gene            7173..7934
                     /locus_tag="PKM80_01635"
     CDS             7173..7934
                     /locus_tag="PKM80_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV92637.1"
                     /translation="MQIPFNMNRLKDTELRYPQRFANMLTKNYGLIFFNEGNKASQDS
                     NHAIIQDLIGVESSLRDIESFYKSKGIRPCLYPSLTQKELAVLAPLIENHNFKLVMKN
                     SEFFLHDHESSLREIDDLKIERVHKLDIDIMETIAIEYGGDWTIRVVERHLLHPSYHF
                     LGGYYKGELATMASISIFAGYTRIDDVFTRDKFRGKGFAGAMIHHLVNYHSRISPNYL
                     YVQSDLPSTIKIYEKAGFSCLPRDFDCWIAHKENN"
     gene            8239..8961
                     /locus_tag="PKM80_01640"
     CDS             8239..8961
                     /locus_tag="PKM80_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNV92638.1"
                     /translation="MIGQRIKQIRTALGIKQVELAKVLKMNPSAVSQIESGRTNPSLE
                     TLSELGKIYNINLHWLITGTGEMFNTIAETESQSGAWDNFQKLMNERLEEIVQARKDL
                     MDSDIVEIAVSGEIAAGAPIENYGASLEVVTVSRSLINGSLSDFVALRVNGRSMEPDI
                     RNQDVVLIRLSNEWRELEGKICAIRIDGGITLKRLLLDDARKMVVLVPINEDFQPIIV
                     DPDSHSDVTLIGSLYFLFRILS"
     gene            8980..10353
                     /gene="rmuC"
                     /locus_tag="PKM80_01645"
     CDS             8980..10353
                     /gene="rmuC"
                     /locus_tag="PKM80_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNV92639.1"
                     /translation="MSSIYLLLLALILLSLLNLYILLFHRRKESRTDNLNELQNAFNS
                     FEHYNREEISKLRTELLNISAENRKELNESIRAQNEVLIQQSTTSREELNNSFNALKQ
                     QFNNDAATNREELKKALNDLSDNFSRKMTELINIQQQEAENSRKNLESKMEQIRQSNE
                     TKLEQMRQTVDEKLHDTLEKRLGESFKQVSERLELVHKSMGEMQNLANGVGDLKKALT
                     NVKTRGVLGEIQLENLLEEMLTPEQYEKNFRPNKNRDEKVEFAIRLPGKSDEQDFVYL
                     PIDAKFPIEDYYRILDAYDLGDLASVENAQKSLVQKIKNCAKDIRDKYINPPITTDFG
                     MLFLPFEGLYAEVLRNTGLFETIMRDYQVIICGPTTTAAVINSLQVGFRTLAIQKKSS
                     EVWKILASIKQEFGKFGTLLDQTQKKLQEASNTIEKASHRSRQIEKRLRKVQELPVQE
                     TEQIMDV"
BASE COUNT         3412 a         1867 c         2037 g         3295 t
ORIGIN      
        1 taaagtcgac taatcgtcag ttataaaata caatcctctg aatcctaaca cccttaaatc
       61 cttgtttcta aagctaaatc tgcgtcatca gcgtaatctg cgtgaactaa tccttaaatc
      121 cttgttccga aaatccaaat ctgtgtaatc tgtgagagat aaaaaaaccg gaggaacagt
      181 gtcctccggc tacccaatat tatttagtat tctttattaa aatcccggtt ttcctcgcgg
      241 atttgttttt cctgtaattt agctgccaga tctatcagtt ttaaaaattc agttctatag
      301 ccctcggtat ctttgccgat atttttggtt gccagttctt taaccatatc ccaattgagg
      361 gctgctttat attgagaatc ttgcaaaagc attccatagc caataacgga acaggcgaaa
      421 agataatttt cggaggcatt ttccaagtgt atagttttat tcataaccgg cacttcaaaa
      481 ggaatgctag tatcgctatc cggaagttta taacgcaatt taaccgttaa tgcctcaggt
      541 gatttacgcg cctcatcgga aatctttata tcctgatatt tcagttcatc cagttcggga
      601 ataggttctt tggaagcggt gggaataatt tcataaacag ctgtaacaca atgtcctgca
      661 cccaattcac cggcatcttt ggtgtcatcc ttaaagtcct ctttattcag cagacggttt
      721 tcatagccga ttaaacggta tgccttaata tgagcgggat taaattcaat ttgcagtttc
      781 acatctttgg ctacggcata aagagtgctt gccatttcat taaccagaac ttttttagct
      841 tcactgatgt catctatata agcataattg ccatttcctt tatcagccaa aagttccaag
      901 gtgttatcct tataatttcc cattccatat cccaaagcgg taaggtaaat gcctttattg
      961 cgtttggatt caattaattg agttaaatgg gcagaggaag atgcgcccac attaaaatca
     1021 ccatcggtac aaagaataat acggttgttt ccacctttga taaaattctc ctcgctaatt
     1081 ttataggcaa gttcaattcc tgctcctcca gctgttgatc ctcctgcctc taatctatca
     1141 atagcatcat aaatatcttt tttttgtcta gcactagtgg aaggtaaaac ttctcctgca
     1201 gctccggcat aaacgacaat cgcaatttta tcctcatccc gtaaattatc caccaacaat
     1261 ttcattgatg cttttaccaa aggcagttta ttcggttcat ccatagaacc ggaaacatcc
     1321 agcaggaaaa ctaaattact gggtgcagct ttagaaaaat ccagcttttt tgcctgcagg
     1381 cctattaaaa ccaagttacg attttgattc caggggcaca cactcatttc agtataaacc
     1441 gaaaaaggat gttccccttc cggcttagga taattatagg caaaataatt taccagttct
     1501 tcaatgcgga tgcttttggg ctcaggtaat tgaccttgtt gcaaaatacg ccggatgtta
     1561 ctatagcaac cagtatccac atctatggaa aaagtggaaa gcggttctgc taaaggactg
     1621 tgaaatatat tgggtttgat tgcttcatat tgttctgtgt taaaaggagt tatatattga
     1681 ttttgcatac tagcggaaag taatgtctgc cacggcacta catccatatc ggaaacagca
     1741 ttgaccttta cactctccat tatgattcca gttttttgca ttgttattgt aagagtggtt
     1801 gttttccctg cctcaattgt gatattactc ttttcaactg tattataacc aattaatgaa
     1861 cattttaaag aatatgtacc agcaggaata tttaaaataa ctgctttccc ttgggcatct
     1921 gtctgaccac ttgttaggcg tttattattg tcataaatag ctacatcagc atattgaagg
     1981 ggagtattat tatcggtaac ggttatggat agcttacctg tttccgtttt cttttcagga
     2041 acaggttttt gcggattggc aaccagcaca atatttaata aagcaggttt attggctttt
     2101 atcttcactt tcttattttc ataaaccgca aagttgggat cgtttatctt aatggtgtaa
     2161 gttcctggtt ttagtttatc aaatcggtaa aaaccattct ggtcggaaac agcggtggca
     2221 ataacagtgt ttttttgcag acattgcaca gtgagatctg ctacaaaggt atttttacct
     2281 gcctcggtaa tagaaccata gatagaaccg tatttttctt ttttagctgc catcaataag
     2341 catagcatta gcagcaaagg aattatccaa tagcgtgttt tcatttttcc tccatacttt
     2401 tatccatttt ttatagttta atgcataatc attatttgtc aagcaattat tgaagtttac
     2461 ctgtaaacgg tttatgcgag taagaaagaa atacagtacc taatagctaa ttttttaata
     2521 ccttttccat atctctattt accatttttg ataactatta attacactaa tttgcatcac
     2581 cctttttgct tgacattatg cacctcttga aaaagatgac ccaaacttta tgtgcatctt
     2641 tagctcagct ggcagagcaa ctgactctta atcagtgggt ccggggttcg aatccccgaa
     2701 gatgtaccat ttttaaaata gcggtgattt gccctttgct ccgcagccaa aatgtgctaa
     2761 agtatggctt tataagtttt gccggcttta gcagccggtt ttttttagat ataaaagggc
     2821 aacttaagca gataaattat attaggagat acaaatgact gcagcattac atgcacttga
     2881 caaaacggac agaaacggct atgttttcac ctctgaatct gtctcggaag gtcatccgga
     2941 taaagtttgc gatcagatat cggatgctat attagatgct tatttagcag ctgatcctca
     3001 ttcgaaggtt gcctgtgaaa cattagctac ggaaaacaga ataatcctat caggagaaat
     3061 ttcttcaaaa gcgacagtag atattgaatc tattgtccgt aaaacaattc aagaaattgg
     3121 ctatacctcc cccaacaaag gttttgatta taattgccaa ataaacaatt acttacaccg
     3181 tcaagaaagc gccctggaag aaaataccgg agctggagat caaggtttaa tgtttgggta
     3241 tgcttgttcc caaactcaaa atatgatgcc tttgccaatt gaattagcac ataagttact
     3301 gttcaatctt gctacggcac gcaaaaaagg aacaataaaa catctgctcc ccgattctaa
     3361 aagtcaggtt tctatgcgtt atttaggaag aaaagctgta gatttgaaat gcttggtaat
     3421 ttctacccat catgatattt taactgaaat ggaatttgag gagctaaagg aacagataca
     3481 gcagctgatt ataattccca gcataagaga tatcgaagat ggctgcaagg gttattttga
     3541 aactaagaat tgtgaaataa aaattaatcc cttaggtcct tggcaggatg gaggccccgc
     3601 ttttgatact ggccttacag gaagaaaaat aattgtggat acttatggag gttgggctca
     3661 acatggagga ggagcttttt ctggaaagga cgccactaaa gttgatagaa gcgctgccta
     3721 tatgggccgt catattgcta aaagtattgt agccagcggt ttagctgatg agtgtttaat
     3781 tcaatttagc tttgtgattg gtgaacagaa accaatatca ttgatggtag atacttttgg
     3841 cagtggcaaa gaaagcgatg cagaaattga aaggattatc gcaaaaaact ttgacttgac
     3901 agttagcggg atcatagatt atttaaattt gcgtaaaccc atatattttc ctactgcctc
     3961 ttacggtcat tttggcagag ataatttttc ctgggaaaga gtaaaaaaaa ttaccagatg
     4021 atggagaaaa cagcatagcc tagctgattg catactgatg agtaaactat atactgacct
     4081 tgcaaatagc caaaacttct ttctaattgc gggaccctgc gttattgaag aagaaagaat
     4141 aatgtattcc attgcagaaa cactaaaaga gctttgtact cgaaaaggga taccgctgat
     4201 ttttaaagca tcctataaaa aagcaaatcg cagttctgta gattctttta gtggtcctgg
     4261 tttagaggaa gggctacaaa ccctggcaaa gatcaaacag gattttgagc ttccagtgct
     4321 aactgatgtc catgaatgta tagaaattac ttctgctgct gatgtttgtg atattttaca
     4381 gattcctgct tttctatccc ggcagacgga tttgataaaa aatgctgccg ccactggaaa
     4441 gataataaat atcaaaaaag ggcaatttat ggctccagaa gatatgcaat ttgctgccga
     4501 aaaagcaaga agcacaggta atgagcaaat tttattaact gagcgcggaa ccacttttgg
     4561 ctaccataat ttagttgtag attttcgcag ttttactatt atgagtcaat ttggctatcc
     4621 ggttgtttat gatgtaactc attctctgca aaggccttcg ctaagcaata tttctggtgg
     4681 caatccagaa tatgcttttt caatggctaa agccgcaatt gccacaggaa aagtaaaagg
     4741 gctttttata gagactcatc cagaacccca aaaagccaaa agcgatgctg ctagtatgct
     4801 ttctctggat aaaatggaat ccctcctgga tggctgttta cagttattaa actgaaggat
     4861 cacagatatg tacgataaaa gagcattaaa tagacccaat aaaagagtag ttccctggaa
     4921 tgaaattaaa ctgcttgttt ttgattgtga tggagtttta acagacggac gcttcgttta
     4981 cggaactaat aatctggaaa tcaagaattt tcacggacac gatggtttgg gtttcaaact
     5041 tttacaacat acggatataa tagtatgttt tataacagga cgcagttccg aggcattgga
     5101 aagacgctgt ctcgatttaa atatcacata tctttttcag aatgttgcca acaaaaaaga
     5161 gtgcttggat aatctattaa aagacttaag cttaacttat actaatgtag cttatatggg
     5221 tgacgactgg aatgatgttc cggtaatgcg aaaagtagca ttttctgcag caccggctga
     5281 tgctcaacct ggaattattc atcttgctga ttgggtttcc gaatttaaag gtggtcacgg
     5341 tgctgcaaga gatctgataa actatgtcct tattcataaa ggcatctatg aaaaaacgat
     5401 tctgtcctat ttggatagca tcggttagtg ttgttattct gctttcttcc tgtcaaaaag
     5461 tagatttaca atctcatacg ggtgttttag cacgtgattt accggatgaa attagttata
     5521 aagttcagct aacccagctc aataacgacc acaatgagta cattttagaa gctgaaagga
     5581 ttgagaggtt ttatgatagg cgtttacttt atgcttataa agtaaccttg accacttttg
     5641 ataaaaacaa tcagataagc tctgttatta aagcggatac aactattgta gatgatgctc
     5701 gtaatctaat ttttgccaat ggaaatgctt ctctaaattc acctaatgga actttatcta
     5761 ctcctagaat ggcttgggat agaactgttg atgaaataac tgccccttta gaagtaactg
     5821 ttactcgtaa aggaaatgtg atgtatggta gaaatttaag aactaacagc aagcttagtt
     5881 ttgtagagat ggatgctgtg tctgcagaag gatattttaa ggaagaggaa ataaactggt
     5941 gaaaaggatt ttgtcttgtc tgatactgtt tttgatgctt ttttctttat atgcaaaagg
     6001 gcaggctgat gaaaaatttc gcttaattca ttccgataaa ctgtttatga ctaaggcagg
     6061 agaggaacag gcacttgaat tgaacggtaa tgttcatttc ttttatggta ataccgaatt
     6121 cttatgtaga aatgccctga ttttagatac tcaaaaaata gctcgtctct ctggaaatgt
     6181 aattgttaag aatgacagtt taaccttgga agcagatact ttagcttatt atcgcattcc
     6241 ggatttgatg aatttgggtg gtagaatcac tgctactgaa aagacaaaaa caggaatcta
     6301 tcgctggata aaaagcaatt ttgcctctta cgataaagca aacgatattt tgaccacttg
     6361 gaacagagta aacagttttg attatcaaga aaacgctcat atccaatgtg gatatgccca
     6421 atgggataga aaaaagggtt atgccatctt actggaagag ccatttataa cttcaggaac
     6481 aaaagatacc ctttctgtag ctgccgaaaa aatggagtat tttgatcagg aacataaact
     6541 gattgctacc tttaatgtaa aagtagagag aaatgaattc catgctacca gcgattttct
     6601 catctatttc ctgaatgaag aaaaagcaat ttttactggt gaaccaaagt tcttttccaa
     6661 ttttgcggat gctaaagcta tggaatttca tctgttttta aaagaccgct ccctaatgcg
     6721 agcagaatta atagattcct gccatatcga ttttgccgaa gaacaattta tggaaaagaa
     6781 aaataaggta gatgcagact tagttactat ggatttttta gatgaccaac tccgtatttt
     6841 tgtggccgaa gggaaggtag attattattt tcaacaggat caaacagaaa agaaggactt
     6901 tatgatcaat agtgctactg gagcatatct gcaagccaac tttgatgata acagcaaact
     6961 gcaatctctg caaatgaaaa ctgatattaa ggggaaatat gtgtttcaac agtaaggtcg
     7021 cttaatagaa agaacctaag ccctttttgc cgtatatttc tgaaaaaccc actttattat
     7081 ttcttcattt ctccaagttt tatttgctct ttctgaaaaa agcaatatct tttaaataga
     7141 gttaaatttt tttatgtgag aaggagttag atatgcagat accatttaat atgaatcggt
     7201 tgaaagatac ggaacttcgc tacccgcagc gttttgccaa tatgttaacc aagaattatg
     7261 gattaatttt tttcaatgaa ggcaacaagg catctcaaga tagcaatcat gctataattc
     7321 aagatctgat tggagtggaa agttctctgc gtgatataga atccttttac aaaagcaaag
     7381 gtatacgccc ctgtttatat ccctctctta cgcaaaaaga attagctgtt ttagcaccgc
     7441 taatcgagaa ccataatttc aaactggtta tgaaaaacag tgaatttttc ctgcacgacc
     7501 acgaaagcag cttacgagaa atcgacgatc tgaaaattga aagagtccat aagctagata
     7561 ttgatattat ggaaactatc gctattgaat atggaggcga ttggactata cgagtggtgg
     7621 aacgtcattt actgcatcct tcctatcatt ttttaggcgg ctattataaa ggtgaattag
     7681 ctactatggc ttccattagt attttcgccg gttacacccg aatcgatgat gtttttacca
     7741 gggataaatt cagagggaaa ggtttcgccg gagcgatgat tcaccatctg gtaaattatc
     7801 acagtagaat ctcgccaaac tatctttatg tgcagtccga tttgccctca actattaaaa
     7861 tttatgaaaa agctgggttt agctgtctgc ctagagattt cgattgctgg atcgcacata
     7921 aggaaaataa ctgatttatt tacctgaaga attttgcaca tagtgcttag tcgcctaaat
     7981 attacccttt tgtcctatac tgaacctaca aaattcatta ttgcttaatt caatgccttt
     8041 tatccctgtg ttttgttttt taatttagct atctaccggt gggtaatttt agtgctactt
     8101 tatattctac gatagaaaag aggataaata ttaaatcagc tataaattac ttgacagctt
     8161 tagcaatgct aataattgtg gatatagatg ttagatatgc taccaactta gtaactctaa
     8221 catcataagg agtgtgatat gattggacaa agaatcaaac aaattcgcac tgctttagga
     8281 attaagcagg ttgaattagc caaggttcta aaaatgaatc catcagctgt gtctcaaata
     8341 gaaagcggac gcactaatcc ctctctggaa actctttctg aattgggtaa gatatataat
     8401 atcaatcttc attggctgat tacaggaaca ggagaaatgt tcaatacaat agcagaaact
     8461 gaatctcaat ccggtgcctg ggataatttt caaaagctga tgaatgaacg gctggaagaa
     8521 attgtccaag cccgcaagga tttaatggat tcggatatcg ttgagatagc ggttagtgga
     8581 gaaatcgcag ctggtgcacc catagaaaat tatggagctt cacttgaagt tgtaacagtt
     8641 agtcgttcat tgataaacgg ttctttaagt gattttgttg ctctccgagt taatggaaga
     8701 agtatggaac cagatatcag aaatcaagat gtagtgttaa ttcgtcttag taatgaatgg
     8761 cgcgagctgg aaggtaaaat ttgtgcaatt cgcatagacg gtggcataac cttgaagcga
     8821 ttattacttg atgatgcccg aaaaatggtt gttttagttc cgatcaatga agattttcaa
     8881 ccgattatag ttgatccaga tagccatagt gatgtaacat taattggatc gctatatttt
     8941 cttttcagaa tattaagtta aagtgaggta taaagcataa tgtcttctat ctatcttctt
     9001 cttttagcac ttattttgct atctttattg aacctctata ttttgttatt tcaccggcgc
     9061 aaagaaagca gaacagataa tttaaatgaa ctgcaaaatg cttttaatag ttttgaacat
     9121 tataaccgtg aagaaatcag caaattacgc acagagcttt taaatatcag cgcagaaaac
     9181 cgtaaagaac taaatgaatc tatccgagcc caaaatgaag tgcttattca acaaagcact
     9241 accagccgag aagaattgaa taattccttt aatgccttaa aacaacagtt taataatgac
     9301 gcagccacaa atagggaaga actgaaaaaa gcactgaacg acctctccga taatttttcc
     9361 agaaaaatga cagaactgat aaatatccaa caacaggaag cagaaaactc cagaaaaaat
     9421 ttagaatcca aaatggaaca gatccgtcaa agtaatgaga ccaaacttga acagatgcgt
     9481 caaacagttg atgaaaaact ccacgatact ttagaaaagc gtttggggga aagttttaag
     9541 caagttagtg aacgcctcga gctggtacat aaaagtatgg gagaaatgca aaatttggca
     9601 aacggagtag gcgatcttaa aaaagcactt actaatgtaa aaacacgtgg tgttttagga
     9661 gaaattcaac tggagaattt gctggaggaa atgcttactc cagagcagta cgaaaagaat
     9721 tttaggccca ataaaaatag ggatgaaaag gttgaatttg ccattcgttt acccggaaaa
     9781 tcagatgaac aggattttgt gtatctgccc attgatgcaa agtttcctat tgaggactac
     9841 tatagaattt tggatgcata cgatttggga gatttagcct ctgtagaaaa tgcccaaaaa
     9901 agtttggtgc aaaaaattaa gaactgcgcc aaggatataa gagataagta tataaatcct
     9961 cctataacaa cagattttgg aatgctattt ttaccctttg aaggtctgta tgctgaagtt
    10021 ttaagaaata ctggactttt tgaaaccatt atgcgtgatt atcaggttat tatttgtggt
    10081 ccaactacta cagccgctgt tattaatagt ttacaagtag gttttcgaac tctggctatt
    10141 cagaaaaaaa gctctgaggt atggaaaatc cttgcctcta tcaaacagga atttggaaaa
    10201 tttggaactt tactggatca aacccaaaag aaactacaag aagcatctaa tacaattgaa
    10261 aaagcaagtc atcggagtcg ccaaattgaa aaacgcctga ggaaggttca ggaattgcct
    10321 gttcaagaaa cagaacaaat tatggatgta taatatcatt tatgagtgag ataaatgccg
    10381 agcgctaatt ttcctgcttc gtaattatcc ttcctgagca agcataaaac catacagaaa
    10441 gtaataatgc ctaattgatt gaaaatagac tttaagcacc tggcaattag gtttcgaggg
    10501 gcttaagcca ccatcgcttt cattgtgtcc gtctttgagt taaaatcggt tttgtttgtg
    10561 attttccaca taatttcatt ctttttccct aactttgaaa aaagtcacta t
//
LOCUS       DAORHB010000028        10384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_5705_length_10384_cov_5.216962, whole genome shotgun sequence.
ACCESSION   DAORHB010000028 DAORHB010000000
VERSION     DAORHB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5705_length_10384_cov_5.216962"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /gene="cls"
                     /locus_tag="PKM80_01650"
     CDS             <1..1470
                     /gene="cls"
                     /locus_tag="PKM80_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNV92640.1"
                     /translation="GGLVLETFSKVTIRIINILFGISIVLIILIVVMENGSPATTMAW
                     ILVLIFLPIIGFILYLFFGRNWRKERLFSKKDYADTQLLATYIQKYPLTIEDNWQTEL
                     SARLNKLLKNNSKATLTVNNEVKLYSDTMEAFNAILKGISSAKHFVHMEYFSIFSDET
                     GYDMQKILIQKALEGVEVRFIYDDVGCWMLKKEFKQELHNAGVEFVPFMPVWIPFLNS
                     RINYRNHRKLVIVDGKKAYLGGLNIGDKYLGKNSYYGYWRDSLAEIEGQAVLSLQAIF
                     LADWNFVTGKNLMQETLLDKYLPTDITVKDKFLPMQVVANGPDSDYASIMQLYFAAIT
                     YAKHNIRISTPYLILNETLLSALKIAAISDVKIQIIMPDKPDHFLVFWASRSYFQQLL
                     DVGVEIWTYHKGFNHSKILIVDDEVLLIGTVNMDLRSFSHNFELAATIYDRNVCAEAI
                     MHFEEDLMNSSKINAEEFKQRSMLYKTRESICRLVSPLL"
     gene            1631..4450
                     /locus_tag="PKM80_01655"
     CDS             1631..4450
                     /locus_tag="PKM80_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNV92641.1"
                     /translation="MKFISYPLSRNLDRIVQELAKPNSILVFPTLIAAQRAEREYLRN
                     WDLSRCNFYSVEELRSALILPQLPIVTDEKRLLCLYMVMEESERDFFHIYSYSDIVEW
                     GRRFFDFFEELCVECIEMETLEQLPNSGIMSMQEWQEKYLEKIIAIRNNYFQFISSLG
                     FSDAIFYLKSENIIVPWQGCEVIYINQYYYSALEKQQIEALEDAGNQVTIVTHGLEIS
                     GETDDWKVQDFDLKNAWNSLERKPVIEILETENETQMALSFLSWNMDNNCPNSAIIDS
                     SFHLKSYSHFFPEEHFAKPDNYPFSDGTIYKMLSAIYKGLKASKESDGYLPVRILANY
                     ISEDWFCHYFFEENSNLEREDYIQQLRLELSDIINKDTLYVNCAFFNNINKPVLQSLV
                     LQYNNLMEAFTEATNLSEICSLIDSPNGLCLIKLLQEHEKQYTDIIPVFWTQMANFMA
                     IENLGLINSWKQIFEEDTLGENLLDLLLNYLKSAKISIQRNEKLSPEWEISNLLDSRN
                     RAITKVAFFQMIEGIVPSNPTPVWLFNESQRAKLGLKTYNDVRKWERYYFFRLLLCSE
                     QAICLSYANQERDISPSSFLGELEELLQNEDVSFLQKKMVGVSLTEVYSENTPVKTIP
                     ELENNEYCCVEKLPEEDFFIIPSDPMTDFPKSNIRFSASALIQFMKNPFLWYVENKCK
                     LYTQNWEAEETISYKLFGNIMHSYFSTTLSKLTGRHISTDALEAFFGNSAKLEAQLRE
                     VITSDKLIYKIPKNYNAEFLGDILAKKLAQSLYLFYKDWLKKQVEYRTFDLYPETDDD
                     SNQVVEYKKLGTVSFQERDYLLYIGGRADLRVELDNKAIIVDFKTGSHDYRQLCIYEW
                     YYYLLDEILPADNVSSLFWNILDPTKKTEGVNEEKRQKLKEQIFEHFTYCLANGYSTI
                     TKVSDRQRLQYITRADLLREIRGFE"
     gene            4447..7344
                     /locus_tag="PKM80_01660"
     CDS             4447..7344
                     /locus_tag="PKM80_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV92642.1"
                     /translation="MIENNCRIICASAGTGKTYRLSLEYLALILKYYGKSEFSLDNIL
                     VLTFTRKATAEIRERIIKHLELLLSDNVEDMDEQNILLNNLRELMEERFVPLSEIEKN
                     MLLSARKELICDQSHLQVMTIDSYTGQIFRNIVRPICNIEHYDIDTEAIIKIMPLLFK
                     HLMKPIFRESLQDLLSKKVSRSLDDYTSFFRSLIEQRWLYYLITKRKSTRDSSSNGIL
                     MNLDPDADSTEYYGNFLNAMQELIQIVVNICAEKNEASLADYFNKEFKQLVNIESNTI
                     NAIYIMDNLKRLDLDAAEKLLTILDKSNIWNKQKISAKKYTVENTQLQNAQDMAKLFL
                     ADYLILKLYIPEQKQILGIWSDVLKEYDRLIYRYKNLTYNDITWLTFEALFSNEPPFL
                     NPQSADSATEFYQFLTHRTRFLLIDEFQDTSLIQFNVLKPIIEEITAGEGSKPFGGVI
                     VVGDEKQSIFGWRGGERDLLLNLKDIFPALKEVKMESLKQSFRCGPTLMEFINQVFSN
                     SALHQLLQEKEMQWNYARIESQEYKKEEGSEIEFCLRNLSKSADNTNKEKDIYQDFIE
                     CMVMPALRKDETGSIAILCRTNNELTKIQHVLDEYKITSLYQPNSSIIEHHYVAPLLS
                     WLRFQAWGDWMDFIAFLRSDYVLINTKVLKQTLAIIARTLELQKEWEKPILPDFSALP
                     IINTLFQLSLQQQGKKATEICRDLIDFCLPSKQLSERDYLNLQRFLDILATRMLTDPE
                     KGTSFPDLLAYFNENANSENFQQVSVNISDSLQLLTFHKSKGLQFKRVFVFYNFYGKH
                     RDTSDTISWALSYEDKTFNKIRDYGISLHYQKIMKLSSYAPLYLQEQKREQLEEMNNL
                     YVAITRAEQKLHLYFGFQYKEGWDAYYDKYKSESLPVAICNAVQEIFMKSTPDERGVY
                     VQKSMFELCEKDKTEKSERSKAIREQINSSIKTVIIDLLILQTIFLQKFQI"
     gene            7410..7787
                     /locus_tag="PKM80_01665"
     CDS             7410..7787
                     /locus_tag="PKM80_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92643.1"
                     /translation="MSFIIRNELKEHQYALYRCLNRYGAIFTGSQIDNFAARCKQVCE
                     HNPYLFTTRWNEIFTEQEIVENSKIFRIDRLMINTAEKESLIIDYKSGDIHDPKQLIN
                     YKNALLKLPALQNYQIDTKIVPL"
     gene            8013..8279
                     /gene="purS"
                     /locus_tag="PKM80_01670"
     CDS             8013..8279
                     /gene="purS"
                     /locus_tag="PKM80_01670"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNV92644.1"
                     /translation="MLKAKIYVKLKASVLDPQGKAVTNSLHNLGFTDVLETRISKYIE
                     MNFTSDDREKVSQEVEYICNSLLANPNTEIYQYEIVPLEQNLEN"
     gene            8269..8955
                     /gene="purQ"
                     /locus_tag="PKM80_01675"
     CDS             8269..8955
                     /gene="purQ"
                     /locus_tag="PKM80_01675"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNV92645.1"
                     /translation="MRISVVTFPGSNCDYDAYRVCLSRGHQTKLIWHKDTDLANPDLV
                     ILPGGFSYGDYLRGGALARFSPIVNEVISFAKKGGLLLGICNGFQILTECGLLPGTLL
                     MNESLLFICKHQYIRVETEASPFTQSIGKGKVLDVPIAHKEGNFYTDSVTLKQLQDNN
                     QIIFRYCDKQGEINLQSNPNGSLDNIAGICNPQRNILGMMPHPERSATDTVPTQDGKW
                     IFEAIEKALE"
     gene            8936..9634
                     /locus_tag="PKM80_01680"
     CDS             8936..9634
                     /locus_tag="PKM80_01680"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNV92646.1"
                     /translation="MKKLWNNFLELIVPPACLVCGNRIEDRTQVICEFCEAKLSLIGE
                     GTCPVCGSENKELPCEVCLDGQFEFDSARSIFRYIGPIRELIHILKYEGYTYPAGYFA
                     LPIAELIESEPSLKNHDYICAVPLHRVRKRERGYNQTDLIAYAVSKLICMPYLNPVTR
                     RINTFSQTLLSKEHRIANLSGAFTVKHKDQVTGKKIILIDDVFTTGSTLNEIAKTLRA
                     AGAKKISTITVARA"
     gene            9637..10026
                     /locus_tag="PKM80_01685"
     CDS             9637..10026
                     /locus_tag="PKM80_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92647.1"
                     /translation="MNKEYLEIRPETSESEINEMIEKVAKFIAERHLAPAGIMFFESI
                     RPLHGIGSQALFMILPIAEIIFDSRKYQQFAIMMEDEKNLKKLISRMDELDEEYNRER
                     RKENRLKRQHRKAKRKAFFRKIFKTKK"
     gene            10052..>10384
                     /locus_tag="PKM80_01690"
     CDS             10052..>10384
                     /locus_tag="PKM80_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNV92648.1"
                     /translation="MQYDEKRLTRIVATDCGSTTTKAILIEWVDGEFRQTLRGEAPTT
                     VEAPLNDVTKGVINATQELEELARLKYHNPNIKFMENGEFVIPRKGDVGVDAYVSTSS
                     AGGGLQMMV"
BASE COUNT         3495 a         1714 c         2004 g         3171 t
ORIGIN      
        1 ggaggcttag ttttagaaac tttcagtaaa gttactatac gcatcatcaa tatcctgttt
       61 ggtatctcta tagtattaat cattcttatc gtagttatgg aaaatggttc tccagcaaca
      121 acgatggcat ggattttggt tctaatcttt ttaccgataa ttggctttat tttgtatttg
      181 ttttttggca gaaattggcg aaaggagagg ttattcagca aaaaggatta tgctgatact
      241 cagttgctgg ctacctatat tcaaaaatat cctttaacca tagaagacaa ttggcaaaca
      301 gaattgagtg caagattaaa taagctttta aagaacaata gtaaagctac ccttacagta
      361 aataacgaag ttaaattata ttccgatacg atggaagctt ttaacgcaat cctgaaaggt
      421 atatcctctg caaaacactt tgttcatatg gaatattttt caattttttc cgatgagaca
      481 ggttatgata tgcagaaaat tcttatccaa aaagcccttg agggagtgga ggtacggttt
      541 atttatgatg atgtaggttg ctggatgctg aaaaaggaat ttaaacagga attacataat
      601 gcaggagttg aatttgttcc ttttatgccg gtatggattc cctttctgaa cagtcgtata
      661 aactatcgta atcatcgtaa actggtaatt gtagatggta aaaaggcata tttgggggga
      721 ttgaatattg gtgataaata cttgggaaaa aacagctatt atggttattg gcgtgattcc
      781 ttagctgaaa ttgaaggaca agcagtactt tctttacagg cgattttttt agcagactgg
      841 aattttgtga cgggaaagaa cttgatgcag gaaaccttac tggataaata tcttccgaca
      901 gatattactg ttaaggacaa atttctacca atgcaagtag ttgcaaatgg tcccgatagc
      961 gattatgcta gtattatgca attatatttt gcagcaatta catatgcgaa acataatatt
     1021 cgcatttcta ctccctattt gattttgaac gaaactctgc tatccgcttt aaaaattgct
     1081 gcaatcagcg atgttaaaat acaaattatt atgcccgata aaccggatca ttttttggtg
     1141 ttttgggctt ctcgaagtta ttttcaacaa cttttagatg taggtgtgga aatatggact
     1201 tatcataaag gctttaatca ctccaaaata ctaattgtag atgatgaagt tctattaatt
     1261 ggaactgtta atatggattt gcgtagcttt agccataatt ttgaactggc tgctacaata
     1321 tatgatagaa atgtatgtgc agaggcaata atgcattttg aagaagacct gatgaatagc
     1381 agtaaaataa atgcggaaga atttaagcag cgtagtatgc tgtataaaac ccgagaatca
     1441 atatgtagat tggtttctcc tttattataa agggagaaat atgtgcaata ttataaggag
     1501 aaaaggctat taaccagaaa ggttgatatt ctatcaatcc tgaaactaac ttatccagca
     1561 gctgaaaaaa cataggtgac taaatagcta aagtgtttat tattaataga ttattatagg
     1621 atagaagatc atgaagttta tcagttatcc cttatctcga aatctggaca gaatagttca
     1681 agaactggct aagccaaata gtattcttgt ttttcccact ttaattgctg cccaaagagc
     1741 tgagcgcgaa tatttacgaa attgggatct ttcccggtgt aatttttata gcgtggaaga
     1801 attgcgttct gcactaatat taccccaatt gcccatcgtg acagatgaaa aaaggctact
     1861 gtgtctgtat atggttatgg aagaaagtga gcgtgatttt tttcatatct atagctattc
     1921 cgacatagtg gaatggggta ggcgtttttt tgatttcttt gaagaactct gtgtagaatg
     1981 cattgaaatg gaaactttgg aacaactacc taattccgga attatgagta tgcaggaatg
     2041 gcaggaaaaa tatctggaaa agattatagc tatccgtaat aactactttc aatttattag
     2101 ttctttaggt ttcagcgatg ccatttttta tcttaaatcc gaaaatatca ttgtgccctg
     2161 gcagggctgt gaggttattt atatcaatca atactattac agtgctctgg aaaaacagca
     2221 gatagaagct cttgaagatg ctggaaacca ggttactatc gttactcacg gtttggaaat
     2281 ttcaggcgaa acagatgact ggaaagtgca ggattttgat ttgaaaaatg cctggaattc
     2341 gcttgagcgc aaacctgtta tagaaatttt agaaactgag aatgaaactc agatggctct
     2401 gtcgttccta tcctggaata tggataataa ttgtcctaac agcgctatta tagatagcag
     2461 tttccatcta aaaagttaca gtcatttctt tcctgaagaa cattttgcta aaccggataa
     2521 ttatcctttt agcgatggaa ctatctataa aatgctatct gcaatatata aagggttgaa
     2581 agcatccaaa gaaagcgatg gctatcttcc agtaagaatt ctggctaatt atatatccga
     2641 ggattggttt tgccactact tttttgagga gaacagtaat ctggaaagag aggattatat
     2701 ccaacagttg cgtttggagc ttagtgatat aattaataaa gatactctct atgtaaattg
     2761 tgcttttttc aataacatta ataagccggt tttacaatct ctggtgcttc agtataataa
     2821 cttaatggaa gcttttacag aagcgacaaa tctatctgaa atctgctctc taattgatag
     2881 ccctaatggt ttatgcctaa ttaaattgct gcaagaacac gaaaagcagt atacagatat
     2941 tattcctgtt ttctggacac aaatggccaa ctttatggct atagaaaact tggggttaat
     3001 aaactcctgg aaacagattt ttgaggaaga caccttggga gagaatttac tggatctgct
     3061 gcttaactat ctaaaaagtg ctaagataag tatacaaagg aatgaaaagc tatctcctga
     3121 atgggaaata agcaatctac tggattcgcg taatcgggct attaccaaag tagctttctt
     3181 tcaaatgatt gaaggcattg ttccttccaa tccaactcct gtctggttat ttaatgagtc
     3241 gcaacgggca aaactggggt tgaaaactta taacgatgtt agaaaatggg agcgttatta
     3301 tttcttccgt cttttgctat gttctgagca ggcaatttgt ttaagttatg cgaatcagga
     3361 acgcgatatt agccctagtt cctttttagg agaattggaa gaactattac aaaatgaaga
     3421 tgttagtttc ttgcaaaaaa agatggtggg tgtttcttta actgaagtat atagtgagaa
     3481 tactccagtt aaaactatcc ctgagcttga gaataatgaa tattgctgcg tggaaaaatt
     3541 gccagaggag gatttcttta tcattccttc cgatccgatg acagattttc ctaaaagcaa
     3601 tattcgtttt agtgcttcag cacttatcca gtttatgaaa aatccttttt tgtggtatgt
     3661 ggaaaacaaa tgtaaactct atacacaaaa ttgggaagct gaagaaacaa tcagctataa
     3721 actcttcggc aatattatgc atagctattt ttctacaact ctctcaaaat tgacaggtcg
     3781 ccatatatct acagacgctc tggaagcatt ttttggaaat agtgctaaat tggaagctca
     3841 actgcgagaa gtgattactt ccgataaact tatttacaag attcccaaga attacaatgc
     3901 ggagttcttg ggagatattt tggccaaaaa attagcacaa tctctttacc tattttataa
     3961 agactggctg aaaaaacagg ttgaataccg cacttttgat ctatatccag aaacggatga
     4021 tgactcaaac caagtggtag aatataaaaa attgggcact gtctcattcc aggaaagaga
     4081 ttatttactt tatatcggag gtagagcaga cctgcgtgtg gaactggata ataaagcaat
     4141 aattgtagat tttaaaacag gcagccacga ctatcggcag ctctgtattt atgagtggta
     4201 ctattatctt ttggatgaga ttttgccagc ggataatgtt tcatctctat tttggaatat
     4261 attagacccc actaaaaaaa cggaaggtgt gaatgaagaa aaacggcaaa agctgaaaga
     4321 gcaaatattt gaacacttta cctattgttt ggctaatggc tatagcacaa taactaaagt
     4381 ttctgataga cagcgtttac agtatattac ccgtgctgat ctactcagag aaataagggg
     4441 ctttgaatga ttgaaaataa ctgccgaata atttgtgcca gtgctggaac tggaaaaacc
     4501 tatcgtctct ctttagaata tcttgcgcta atcctgaaat attatggaaa aagcgaattc
     4561 tctttagata atatcctggt tctaactttc actcgtaagg caactgctga aatcagagaa
     4621 cgaattatta agcatttgga attgctactc tctgataatg tagaagatat ggatgaacaa
     4681 aatatattgc taaataattt gcgagaactg atggaggaaa gatttgtccc tttaagtgaa
     4741 atagaaaaaa atatgctact gagtgcccgc aaagaactaa tctgcgatca aagccattta
     4801 caagtgatga ctattgattc ttacactgga caaattttta gaaatattgt gcgtcctatt
     4861 tgtaatatag aacactatga tattgatacc gaagcgatta ttaaaataat gccgttattg
     4921 tttaaacatc taatgaaacc catattccgc gaaagcttac aagatttatt aagcaagaaa
     4981 gttagccgct cattggatga ttatacttcc ttcttccgtt ctttaattga acagcggtgg
     5041 ctatattatc taattaccaa gcgaaaatct acccgggata gttcctcaaa tggaatattg
     5101 atgaatttag atcccgatgc cgatagcaca gaatattatg ggaacttcct gaatgctatg
     5161 caggaactta ttcagatagt ggtaaatatt tgtgctgaaa aaaacgaagc ctccttggca
     5221 gattatttta acaaggaatt caaacaactg gtgaacatag aatccaatac aattaatgct
     5281 atatatataa tggataattt gaaacgcttg gatttagatg ccgcagaaaa actacttaca
     5341 attctggata aaagcaatat ctggaacaag cagaaaatca gtgctaaaaa atatactgta
     5401 gaaaatactc aactgcaaaa tgcgcaagat atggcgaaac tctttttggc ggattatttg
     5461 atcctgaaac tatatattcc cgaacaaaag caaattttgg gaatttggag tgatgttctg
     5521 aaagaatacg atcgactaat ctaccgctat aaaaatctta cctataatga tataacctgg
     5581 ttaacttttg aagccctttt cagcaatgaa cccccttttc taaatcctca aagtgctgat
     5641 tccgctactg aattttatca gtttttaacc catagaactc gtttcctgtt gattgatgaa
     5701 tttcaagata cctcccttat tcaatttaat gtgctaaaac caattattga agaaatcact
     5761 gctggagaag gttctaaacc ctttggcggc gtaattgttg tcggtgatga aaaacaatct
     5821 atctttggct ggagaggagg tgaacgcgat ttattgctaa atttgaaaga tatttttcct
     5881 gccctgaaag aagtgaaaat ggaatcctta aaacaaagct ttcgctgtgg acctactttg
     5941 atggaattta ttaatcaggt gtttagtaat agcgctctac atcagctttt acaagaaaaa
     6001 gagatgcaat ggaattatgc ccgaattgaa agtcaggaat acaaaaaaga agaaggctcg
     6061 gaaattgaat tctgcctgcg caatttatcg aaaagtgccg ataataccaa taaagaaaaa
     6121 gatatttatc aagattttat cgaatgtatg gttatgcctg cgctgagaaa agatgaaacc
     6181 ggttcaatcg ctattttatg cagaaccaat aatgagctta ccaaaattca acatgtttta
     6241 gatgaatata aaattaccag tttatatcaa cccaatagtt ctattattga acatcactat
     6301 gttgctccat tactttcatg gctgcgtttt caggcttggg gtgattggat ggattttatt
     6361 gcttttctgc gttcggatta tgtgttaatc aataccaagg tactaaaaca aactttggct
     6421 attatcgctc gaactctgga attgcaaaag gaatgggaaa agccaatcct accggatttt
     6481 tctgctttac ctataataaa tactctattc caactgtcct tgcaacaaca ggggaaaaaa
     6541 gctaccgaaa tatgcagaga tttgattgac ttctgtttgc cttccaaaca actaagtgaa
     6601 cgagattatc taaatctgca acgctttctg gatattttgg ctacccgtat gttgactgat
     6661 cccgaaaaag gaacttcctt tcctgacttg cttgcctact ttaatgaaaa tgctaattct
     6721 gagaattttc aacaagtatc cgtaaatatc agcgacagct tacaattgct taccttccat
     6781 aaatccaaag gattgcagtt caaaagagtt ttcgtttttt ataattttta cggtaaacat
     6841 agagacacca gcgatactat atcctgggct ttaagctatg aagataaaac ctttaataaa
     6901 atcagggatt acggaataag tttgcactac cagaaaataa tgaaactatc aagctatgca
     6961 cctctctatc tacaagaaca aaaaagagaa caattggaag aaatgaataa tctctatgtg
     7021 gctatcaccc gcgctgaaca aaaattgcac ttatattttg gctttcaata taaagaaggt
     7081 tgggatgcct attacgataa atataaaagt gagagcttac cggtcgctat ttgcaatgct
     7141 gttcaggaaa tctttatgaa atctactccc gatgaacgcg gtgtttatgt ccaaaaaagt
     7201 atgtttgaac tatgcgagaa ggataaaacg gaaaaatccg aaagaagcaa agccataaga
     7261 gagcaaatca attcttccat aaaaactgtc atcatagacc ttttgatcct gcaaactatc
     7321 ttcctgcaga agttccagat ataaaagacc tgaaaaaata ttatctaatt gagcgcccta
     7381 acctgattgg agatttactg catttttata tgtcctttat tattcgtaat gagctaaaag
     7441 aacatcagta tgcattgtat agatgcctaa accgctatgg agcaatcttt acaggttctc
     7501 aaatagataa ttttgctgcc cgctgtaaac aagtctgtga acataatcct tatctattta
     7561 ctaccagatg gaatgagatt tttacagaac aggaaattgt ggagaatagt aaaatattca
     7621 gaatagatcg gttgatgatt aatactgctg aaaaagaatc actgattata gattataaaa
     7681 gcggagatat acacgatcca aagcagctta taaattataa aaacgctctg ctgaagttgc
     7741 ctgccttaca aaattaccag attgatacta aaatagtacc cctataattg taaagtgtca
     7801 acactgctga aatagcttat aatatctctg ttaatcctat gtaccagata aaatacagca
     7861 agataaagaa attgtatcgt tggctgtgag tagatgaaga catcaaccgt cccgggaaat
     7921 ggcctctgct gactctttta aacaaaagta ttgactaaat ctgcaagttt atagaaaagg
     7981 ataaattagt ataattacct aaaggaaaag caatgctaaa agcgaaaatt tatgtgaaac
     8041 taaaagccag cgttcttgat ccacaaggca aagctgttac caattcccta cataatttgg
     8101 gttttacaga tgtcttggaa acccgaatca gcaaatatat agaaatgaat tttacaagtg
     8161 acgataggga aaaggtgagc caggaagtag aatatatttg taatagcctg ttagctaatc
     8221 ctaacactga aatctatcaa tatgagatag ttccgttgga gcaaaatctt gagaattagt
     8281 gttgtaacat ttcccggctc taattgtgat tacgatgcct atagagtctg ccttagcaga
     8341 ggtcaccaaa caaaactcat ttggcataag gatacagact tggctaatcc tgatttggtt
     8401 attcttccag ggggcttttc gtatggagat tatcttcgtg ggggagctct ggcaaggttt
     8461 tctcctattg tgaatgaagt tatttctttt gctaagaaag gtggtttatt gcttggtatt
     8521 tgtaacggct ttcagatttt aacggaatgt ggtttactgc caggaacatt attaatgaac
     8581 gaatctttac tatttatctg taaacatcaa tatatccggg tggaaactga ggccagtcct
     8641 ttcacccaaa gtattggcaa aggaaaagtt ctggatgttc ctatagctca caaggaaggg
     8701 aatttctata cagattctgt aactttaaag caattacaag ataataatca aataattttc
     8761 cgctattgcg ataagcaagg tgagatcaat ttacaaagta atcccaatgg ctcattagat
     8821 aatatagccg gaatttgcaa cccgcagaga aatattctag ggatgatgcc tcatccggaa
     8881 agatcagcta ccgataccgt tccgactcaa gatggaaagt ggatttttga agcaattgaa
     8941 aaagctctgg aataattttc tggaactaat tgttcctcct gcatgtttag tctgcggaaa
     9001 tagaattgaa gaccgcactc aggttatttg tgaattttgc gaagccaagc tttctttaat
     9061 aggagaagga acctgtcctg tttgtggtag cgaaaacaaa gaactaccct gtgaggtctg
     9121 tttggatggt caatttgaat ttgattctgc acgctccata tttcgttata taggtcctat
     9181 acgagaactg attcacattt tgaaatatga gggctatact tatcctgccg gttattttgc
     9241 tttaccgata gcggaattaa tagaatctga accaagctta aagaatcacg attatatatg
     9301 tgcggttcct ttgcatagag ttagaaaaag ggaacgcggt tataatcaaa ccgatttgat
     9361 tgcttatgct gtttcgaaac tgatttgtat gccctatttg aatcctgtta caagaagaat
     9421 aaacactttt agccaaactc tgctctccaa ggaacataga attgcaaatt taagcggtgc
     9481 tttcaccgtt aaacataaag accaggtaac tggtaaaaaa atcattctga tagatgatgt
     9541 ttttaccact ggcagtactt taaatgaaat agctaaaacc ctgcgtgctg caggggctaa
     9601 aaaaataagc acgattactg ttgcccgagc ttgaagatga ataaagaata cttggaaatc
     9661 cgaccggaaa cttcagaaag cgaaattaac gagatgattg aaaaagtagc aaaattcata
     9721 gctgaacgac atttggctcc agcaggaata atgttttttg aatccatccg tcctttacac
     9781 ggaattggca gtcaagcgct atttatgatt ttacctatag cggagattat tttcgattcc
     9841 cgaaagtacc aacaatttgc tataatgatg gaagacgaaa aaaacttgaa aaaactgatc
     9901 agtcgtatgg atgaactgga tgaagaatat aatagagaac gcagaaaaga aaatcgattg
     9961 aaaagacagc atcgaaaagc caaaaggaaa gcattcttta gaaaaatatt taaaaccaaa
    10021 aaataaaatt gcttgaataa gtggaggata tatgcaatac gatgaaaaac gccttacacg
    10081 tattgttgca actgattgtg gcagcactac caccaaggca atattaatcg aatgggtgga
    10141 tggagaattt cgtcaaaccc ttcgcggtga agcacctact actgtagaag cacccttaaa
    10201 cgatgttacc aaaggtgtta tcaacgctac ccaagaactg gaggaattag cacgcctaaa
    10261 atatcataat cccaatatta agtttatgga aaatggggaa tttgttattc cgcgcaaagg
    10321 ggatgtaggt gtagatgctt atgtatcaac ttcttctgct ggaggtggat tacaaatgat
    10381 ggtt
//
LOCUS       DAORHB010000029        10337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_5744_length_10337_cov_4.570998, whole genome shotgun sequence.
ACCESSION   DAORHB010000029 DAORHB010000000
VERSION     DAORHB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5744_length_10337_cov_4.570998"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKM80_01695"
     CDS             <1..405
                     /locus_tag="PKM80_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNV92649.1"
                     /translation="LIGMGKLGAKVTVCGPKTLMPGNMESVYDCRVEYDLPRALKDAD
                     VVMGLRMQLERMTEGLFPSLEEYSKHYVLSKETLKYAKRNALIMHPGPMNRGVEILPE
                     IADSNQSVIVEQVANGVAVRMALMFLILGGKA"
     gene            417..1703
                     /locus_tag="PKM80_01700"
     CDS             417..1703
                     /locus_tag="PKM80_01700"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNV92650.1"
                     /translation="MKILIKNAKIFQKDKFIKGDVLIERKKITKLGGTITVNADKIID
                     AQGACVFPGFIDLHSHLRDPGHTYKEDIVTGTKAAAHGGFTTVCAMPNTEPVTDNIAS
                     VEYIQLRAKDYGSARVYVIGAITKQSAGEEIAEMATMKSGGIVAVSDDGKCVQDARLM
                     LSCMKYASNFGLPIIIHPEDYSLAGKGQIHSGKVATKLGLSGIPGLAEEVIIARDIML
                     AESAGAQLHIAHISTARSLELVKNAKDKGLPVTCEVTPHHLVLNEEATITFNTNTKMK
                     PPLRSEKDRLACVQALKDGLIDCIATDHAPHADFEKEKEYDHAPFGIIGLETAFPVLY
                     KNLVQTGLISLNRLVEALTIAPAKILDLPGGVLEVGKAADLTIIDLEKETLFSVENIL
                     SKSKNTPWLNQFLPGRVLCTICNGNVTYMDSEKWTN"
     gene            1691..2482
                     /locus_tag="PKM80_01705"
     CDS             1691..2482
                     /locus_tag="PKM80_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNV92651.1"
                     /translation="MDKLKPQFLERAIYSREELCGDYFILWIWDEELGKKCQPGQFFE
                     IRAHYSIENAEETRSLPKLFKPISIYDNAEGRIGFFIKKVGYGTNALCKLKAGDLLEL
                     IGPLGNGFPIVSGKKILLVSGGIGYPPLWYLQKRLIKHNLIYWVHGGSNQCDVFPCDE
                     VWTEDGSLGKMGMVTEGVRSLIMEKKINLIYSCGPIPMLKECARIALELEIEHYCSLE
                     AYMACGIGVCYGCAVPIGNTEDWNYLRVCKEGPVFNAKEVLWNRM"
     gene            2467..3384
                     /locus_tag="PKM80_01710"
     CDS             2467..3384
                     /locus_tag="PKM80_01710"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNV92652.1"
                     /translation="MEQNVSQIYTSLGRLTLNSPVTIASGTFALENLDFMAQDYLGAY
                     VCKTITRYPKKGNPPPRLFETEAGLLNSIGLQNPGLDKFINDDLPILRETLTTPLIVS
                     FSGSSLNEFKLMLESLEQNEGIDGYELNVSCPNVENEGIAFGVDPEIVYKLCALLAPL
                     TNRELIIKLSPNVTDIAVIAKAAEDGGATSISLINTIWGMAIDIQSGKSMLKKGIGGY
                     SGIGIKPLALALTYRAAKAVDIPIIAMGGIYSWQDALEFFWAGASAVALGTANFIDPL
                     AVPKVYEGIDNFLRERNCTLNEIIGKCEV"
     gene            3592..5649
                     /gene="fusA"
                     /locus_tag="PKM80_01715"
     CDS             3592..5649
                     /gene="fusA"
                     /locus_tag="PKM80_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV92653.1"
                     /translation="MKEYEMKKIRNLLFIGASGAGKTTLAEQIFYLTKTSNRLGKIDE
                     GNTIMDFDPEEITKQMSLGLSIGYVNYLDHKINILDAPGCPDFVGDTIVALPAVENVV
                     IVANAAGGYEVGLELAIEQIEKSKMGKVIVVNRIDNEHSDYDKTLEAIAENTKIKPVK
                     VHIPIGKEGSFEGVIDIIKRKAIKGKTERDIPANMSEIVEEARMHLMEAVAETDEDLL
                     NDYLENMELPEDKLIAGLKKGIISGEISPAFVCSAGTGEGVLALLSGIIDYLPSPEDS
                     NEMEVLKENEKVSFICKPDGELLGYLFKLYADPSMGDFAYARIFSGTLKTGVEFYIPE
                     KDAKDKAGTMYYMLGKNRNDCNEIKAGDIGALLKLKNAKVMNSIVATGSKLSVVPVEL
                     PTPTTWQTLKAVNQADEDKIGTVLQRVIAEDPTINYELNVETHENVLSGMGEQQLQLV
                     LKKLKNRYKVDAILKDPRIPYKETIMASSESNYKHKKQSGGRGQYADVYFRIKPTERG
                     EGFKFINSIVGGVIPSNYIPAIEKGLIETMEKGIIAGYPVVDISVEVYYGSYHEVDSS
                     EIAFKIASSMALKEGFKKSKPILLEPINELVIIVPSEYMGDVMGDISTRRGKILGMEQ
                     NGKKQYLTAQMPLAEMYSYYPALKSFTQGRGRFTQKFSHYEKVPDDIAQKVIAAWQEQ
                     SNE"
     gene            5867..6622
                     /locus_tag="PKM80_01720"
     CDS             5867..6622
                     /locus_tag="PKM80_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNV92654.1"
                     /translation="MSGHNKWSSIKHKKGAADAKRGQIFTRLVKEIILAARAGGGDPD
                     MNPRLRTAINTAKENNMPKENIERAIKRGTGEIEGAAYEEIIYEGYGHSGVGIVVEVM
                     TDNKKRSVSELRHIFSKYGGNLAETGSVAWNFEQKGFMNVPAAGLDEDDFMMKALEAG
                     AEDVELNEDNFDIYTSPTDFHTVISNLEKEGFPIHNAELTRVPKNTIAVDDDIAEKLM
                     RLIDSLEELDDVQKVYANFEFSDAFMEKLSQAQ"
     gene            6627..7103
                     /gene="ruvC"
                     /locus_tag="PKM80_01725"
     CDS             6627..7103
                     /gene="ruvC"
                     /locus_tag="PKM80_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNV92655.1"
                     /translation="MIIIGLDPGSRYCGYGLLQIEDRKILAAGCDVLDVTKGKTLSLR
                     LKSLYENIIAVLDEYQPEIAAVESIFFHRQVRSVFTLGHARGVILLALAMKDIPIVEY
                     SPREIKKSVCGNGSASKQQVRYMINKIYPLKESPKRDDAFDALAVATCYYNRMKWL"
     gene            7103..7702
                     /gene="ruvA"
                     /locus_tag="PKM80_01730"
     CDS             7103..7702
                     /gene="ruvA"
                     /locus_tag="PKM80_01730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNV92656.1"
                     /translation="MIQYISGTLTFKNPVMAIIETMGIGWEIKIPISTYEVLPSVGKN
                     CKLLTFLHISQDDMRIFGFSTEAERELFMMLNKVSGIGPKIALSILSTLSIPTFIKAI
                     NSGEEGLLTRVPGIGKKSAQRLIVELQDDVHTLMSHIDQKDLQIGDKNSEVESALLSL
                     GFNLQQIRKELALLDESMRQLNTETLIKEIIKRIYQRNK"
     gene            7699..9015
                     /gene="rsmB"
                     /locus_tag="PKM80_01735"
     CDS             7699..9015
                     /gene="rsmB"
                     /locus_tag="PKM80_01735"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNV92657.1"
                     /translation="MNFRTEAYSTILKVLKDKEYSDTLLHQRAKKIKNAGEDPALFYN
                     LVKGVIKLCLKLDYILQQYVDKKKFASTDVKIKTLLYMGLYQLMYLNSIPDHAAVNET
                     VELAKTLFNEQVADFVNAVLRSYLRNPEITYPKEPELRIAYEHSFPPELVKQWLELWG
                     EENTEYLAMFFNENPELHIRVNTTATTPDKLKAYFAKRDIILKPSAASSNMFYTAQTA
                     EVLEDVAFSEGYFSVQDTSAALIVELLDPQKEESVLDLFAAPGGKCSYIAERMNNTGE
                     VIAVDKIPSKMKLLKQAADRLQLTNIKTVITDAFKYGPVAPAFQRVLVDAPCSGWGVF
                     GRKSDLRWQVHQNIPELIKLQEKALDYAANFVAPNGFLVYSTCTMNPKENEDQIASFL
                     KRNKKFELVPASQFIPDKYTSSGFLKTIPFKHNMDGAFAAKLQRIK"
     gene            8978..9610
                     /locus_tag="PKM80_01740"
     CDS             8978..9610
                     /locus_tag="PKM80_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNV92658.1"
                     /translation="MVHLPQNYNGLNKNRYLQGKGKTKNKQIGYLVGIGICVIIVTAF
                     VFSQIFFPIILGRTPKVEVPSVVGMNLAHAKKILQDEKLHAVVKDSLFSDVAAPEVVL
                     EQHPVPGDKLHQDGTVYLVISKGSSTVTVPSVVGKPFQEAFIILRNLDLHSTVVDSTY
                     SDIYPVNTVIRSVPSAGAKALKKSTVKLILSKGSEPTADTLDYEMIPYYF"
     gene            9642..10115
                     /locus_tag="PKM80_01745"
     CDS             9642..10115
                     /locus_tag="PKM80_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92659.1"
                     /translation="MNEELKEIKIRARMMPGAVTYDGFLGNDKRSLSEIIAADNKELE
                     RFNRYVEEIADRMKYFTDATFDVFDNKVVIDDIYEVESTSSKGNLSCPYGHGGLFRKY
                     ETTLTNRKNNITVKWTAMTIHLIEAHHFFGGKGSHYRYEPETLLKALFTEPERKK"
BASE COUNT         3439 a         1633 c         2147 g         3118 t
ORIGIN      
        1 ttaattggaa tgggcaaact gggtgctaaa gtaacagtat gcggtcctaa aactctaatg
       61 ccaggtaata tggaaagcgt ttatgattgc cgcgttgaat atgatttgcc aagagcatta
      121 aaagatgctg atgtagttat gggcttaaga atgcagctgg aaaggatgac agaagggtta
      181 tttccttctt tggaagaata cagtaaacac tatgtccttt ccaaagaaac cttaaaatat
      241 gctaaacgaa atgctcttat tatgcatcca gggccaatga ataggggagt ggaaatcctt
      301 cctgaaattg ccgatagcaa ccaaagtgta atcgtggaac aagttgctaa tggagttgct
      361 gttagaatgg cattaatgtt tttaatctta ggcggaaagg cataaggaga aatattatga
      421 agatcttaat taaaaatgca aaaatcttcc aaaaggataa attcataaaa ggtgatgtgc
      481 taatagaacg gaaaaagatt accaaattgg gaggcactat aacagttaat gccgataaaa
      541 ttatcgatgc ccaaggtgcc tgtgtttttc caggttttat tgatcttcat tctcatttac
      601 gagatcctgg ccatacttac aaggaagata ttgttacagg aactaaagct gctgctcatg
      661 gaggttttac cactgtttgc gctatgccaa atactgaacc agttaccgat aatattgcct
      721 ctgtggaata tatacaactt cgtgccaaag attatggctc tgccagggta tatgtaattg
      781 gagctattac aaagcaatct gcaggcgaag aaattgcaga aatggcaact atgaaatctg
      841 gaggtattgt agctgtatcc gatgacggta aatgtgttca ggatgcgcgt ttgatgctct
      901 cttgtatgaa atatgctagt aatttcggac tgcccattat tattcatccc gaggattatt
      961 ctttggctgg aaagggacaa atccattccg gaaaagttgc taccaagctt ggtcttagtg
     1021 gtattccggg tttggctgaa gaagttatta ttgccaggga tattatgctt gctgaaagtg
     1081 ctggggcaca acttcatatt gctcatattt ccactgcccg atctttggaa ttagtaaaaa
     1141 atgccaaaga taaaggatta ccggtaacct gtgaagtaac tcctcaccac ttagtgttaa
     1201 atgaagaagc tacgataaca ttcaatacaa ataccaaaat gaaaccacct ctaagaagtg
     1261 aaaaagatcg cctggcttgt gttcaggcat taaaggatgg tctgattgat tgtattgcta
     1321 cagaccatgc tcctcatgca gattttgaaa aagaaaagga atatgatcat gctccctttg
     1381 ggatcatcgg attagaaaca gcttttccag tgctctataa aaatctggta caaacaggac
     1441 tgataagttt aaatcgtttg gtagaagcat taacaatagc tcctgctaaa atattagatt
     1501 tacctggtgg tgtactggaa gtaggtaaag cagctgattt aaccattata gatttagaga
     1561 aggaaaccct cttttcggta gaaaatattc tatctaaaag taaaaatact ccttggctaa
     1621 atcaattttt accgggaaga gtgttatgca caatctgcaa cggcaatgta acctatatgg
     1681 atagtgaaaa atggacaaat tgaaacccca atttttagaa cgagcaatat actctcgtga
     1741 agaactctgc ggagattatt tcatactgtg gatatgggat gaagagctgg ggaaaaaatg
     1801 ccaaccaggt caattttttg aaatacgcgc tcattacagt attgaaaatg cagaagaaac
     1861 acgttcgtta cccaagcttt ttaaaccaat ctctatctat gataatgctg aaggtaggat
     1921 tggattcttt attaaaaaag tgggatacgg cacaaatgct ctttgtaaac tgaaagctgg
     1981 agatctactt gaattgatcg gtcctctagg aaatggcttt cctatagtta gcggaaaaaa
     2041 aatactatta gtaagcggtg gaattggtta tccacctcta tggtatcttc aaaaaagact
     2101 tattaaacac aatctaattt attgggttca tggggggtcg aatcaatgtg atgttttccc
     2161 ttgtgatgag gtctggactg aagatggcag cttaggtaaa atggggatgg ttactgaagg
     2221 agtaagatct cttattatgg agaagaagat aaatttaata tacagctgtg gaccaatccc
     2281 gatgctgaag gaatgtgcca gaatagcttt agaacttgaa attgaacact actgttcttt
     2341 ggaagcatat atggcatgcg gaattggtgt ctgttatggt tgtgctgttc ctatcggtaa
     2401 tactgaagat tggaattatt taagagtctg taaagaaggt cctgttttta atgctaagga
     2461 ggttttgtgg aacagaatgt aagccaaatt tatacttcgt tgggacgctt aacattaaat
     2521 agtccagtaa ctattgcttc cgggactttt gctctggaga atctcgattt tatggcgcag
     2581 gactatttag gtgcttatgt atgtaaaact attacgcgtt accctaaaaa aggaaatcct
     2641 ccacctcgtc tgtttgaaac agaagcagga ttgctaaatt ccattggatt acaaaatccg
     2701 ggactggata aattcattaa tgatgattta cctatactta gagaaacact tactactccc
     2761 ttaattgtta gtttttctgg ttcttcccta aatgaattca aattaatgtt ggaatctttg
     2821 gaacagaatg aaggaataga tggttatgaa ctaaatgttt cctgtcccaa tgttgaaaat
     2881 gagggtattg cctttggagt tgatcctgaa attgtctata agctttgtgc acttcttgcg
     2941 ccgttaacta atagggaatt aattattaag cttagcccaa atgtaactga tattgccgtt
     3001 attgctaaag ctgctgaaga tggaggagca acatctatct cgttaataaa tacaatttgg
     3061 ggaatggcaa ttgatatcca aagcggaaaa tcgatgttaa aaaaaggaat tggcgggtac
     3121 agcggaattg ggataaaacc cttagctcta gctttaactt atcgagcagc aaaagctgta
     3181 gatataccta taattgctat gggtggaatt tatagctggc aggatgctct ggaatttttt
     3241 tgggctggtg cttcagctgt tgctctgggg actgcaaatt ttatcgatcc actagctgtt
     3301 cctaaagtat atgaaggtat agataatttc ttgcgagaaa gaaattgtac tctaaatgag
     3361 ataataggta agtgcgaagt ataagtaaaa taatgagcag atataatgaa tattatcgat
     3421 attgctaaaa ttagaaccaa tgtaagcctt aaaagctgca ataatctatg ccttctaata
     3481 agcatacatc ttgggcaaaa taacaattga cttaaatacc aagtaaaaaa aaatggctca
     3541 ttactttaga attatacata aaaaataagg aataaactta ctgaggaaga aatgaaagaa
     3601 tatgaaatga aaaagatccg taatctattg ttcattggtg ccagtggtgc aggtaagaca
     3661 actttggctg aacaaatctt ctatttgact aaaacaagca accgtctggg caaaattgat
     3721 gaaggcaata ctataatgga ttttgatccc gaagaaataa ctaaacaaat gtctttgggg
     3781 ctttccattg gttatgttaa ttatttagac cataaaatta acattctgga cgcaccaggc
     3841 tgtcctgatt ttgttggaga tacaatagta gctcttccag cagtggaaaa tgttgttata
     3901 gtagccaatg ctgcaggtgg ttatgaagtt ggtttagaac ttgctataga acagatagaa
     3961 aaaagcaaaa tgggtaaagt aattgttgtt aatagaatag acaatgaaca ttcagattat
     4021 gataaaaccc ttgaagcaat agcagaaaat actaaaatca aacccgtaaa agtccatatt
     4081 ccgattggta aagaaggcag cttcgagggt gtgatagata ttataaagcg caaagctatc
     4141 aaaggtaaaa ccgaaagaga tatcccggcc aatatgagtg aaatagtaga agaagccaga
     4201 atgcacttga tggaagcagt cgcagaaact gatgaggatt tacttaatga ttatctggaa
     4261 aatatggagc ttcctgaaga taaactaata gcggggctta aaaaaggtat catcagcggt
     4321 gaaatatctc ctgcttttgt ttgctctgca ggaaccggtg aaggtgtgct tgctttatta
     4381 agtggaatta tcgactatct tccttctcct gaagattcca atgaaatgga agttttaaaa
     4441 gaaaacgaaa aagttagctt tatctgtaaa cccgatggtg aacttttagg ctatttattt
     4501 aagctttatg ctgatcccag tatgggtgat tttgcctatg cacgcatatt ctctggaact
     4561 ctaaaaacag gtgtggaatt ttacattccc gaaaaagatg caaaagataa agcaggaact
     4621 atgtattata tgctgggcaa gaatcgtaat gattgcaacg aaattaaagc tggcgatatt
     4681 ggcgctctgt taaagctgaa aaatgcaaag gttatgaata gtatcgtggc caccggttca
     4741 aagttgtctg tggtgcctgt tgaattacct actccaacta cctggcaaac cttgaaagca
     4801 gtgaatcaag ctgatgaaga taaaatcgga actgttctgc aaagagttat tgctgaagat
     4861 cctactataa attatgagct taatgtggag acccatgaaa atgtactttc cggtatgggt
     4921 gaacagcaat tgcaattagt attaaagaaa ctgaaaaacc gctataaagt agatgctatc
     4981 cttaaggatc ctcgtattcc ttataaggaa acaattatgg caagttcaga atctaattat
     5041 aaacacaaaa agcaatcagg tggtcgcgga cagtatgctg atgtctattt tcgcataaag
     5101 ccaaccgaaa ggggagaagg atttaaattt atcaattcca ttgtcggtgg tgttatccct
     5161 tccaattata ttcctgctat cgaaaaaggt cttattgaaa caatggaaaa aggaattata
     5221 gctggatacc cggttgtaga tatttctgtt gaagtgtatt atggtagcta ccacgaagtg
     5281 gacagttctg aaattgcttt caaaatagcc tcttcaatgg ctttaaaaga aggattcaaa
     5341 aaaagcaaac ctatattatt ggaacccatt aatgagctgg ttattattgt tcccagtgaa
     5401 tatatgggag atgttatggg tgatatttct actcgcagag gtaaaattct gggtatggaa
     5461 caaaacggta aaaagcagta tttaacagct caaatgcctt tagcagaaat gtattcatat
     5521 tatcctgctt tgaaatcctt tactcaggga cggggccgct ttacgcagaa attctctcat
     5581 tacgagaaag ttcctgatga tattgcacag aaagttatag ctgcctggca ggaacagtca
     5641 aacgaataat acgatttaac aggtgagaac cctaagttaa atcacaatag atataagagg
     5701 aacggcgttc gcgtcgttcc ctgttttaga aattaaaaaa gctatctcta tttttcatag
     5761 agcaataaat taatctggtt taccgatttt ttctttttta cttgacagta gaagataggt
     5821 ttacatagtg ttcttaagta gtgataaata aaaaaacgag gttaatatgt ctggacataa
     5881 taagtggagt tcaattaaac ataaaaaagg tgctgccgat gcaaagagag ggcagatttt
     5941 tacccgttta gttaaggaaa taattttagc agcaagagct ggcggtggcg atcctgatat
     6001 gaatcctcgc ttacgaactg ctattaatac agctaaagaa aacaatatgc ccaaggaaaa
     6061 tatcgaacgc gctattaaaa gaggcacagg tgaaattgaa ggtgcagctt atgaagagat
     6121 tatttatgaa ggttatgggc atagtggagt gggaatagtc gttgaagtga tgacggataa
     6181 caaaaaacga agtgtttctg aattgcggca catattttca aaatatggtg gaaaccttgc
     6241 cgagacagga tcagttgctt ggaattttga acagaagggt tttatgaatg ttcctgcagc
     6301 aggattggat gaggatgatt ttatgatgaa ggcactggaa gcaggagctg aagatgtaga
     6361 gctaaatgaa gataactttg atatatacac ttcccctacg gattttcaca cagtaatcag
     6421 caatttagaa aaggagggtt tccctatcca taatgcagaa ttaaccaggg ttccgaaaaa
     6481 tactatagcg gttgatgacg acatagctga aaagcttatg cgtttgattg attccctcga
     6541 agaactcgat gatgtgcaaa aagtttatgc caattttgag ttttccgatg cctttatgga
     6601 gaaactttcc caagcacagt aattaagtga ttatcattgg tttagatccc ggcagccgtt
     6661 attgcggtta tggtcttctt caaattgaag acagaaaaat tcttgctgca ggttgtgatg
     6721 tgctggatgt aacaaaaggt aaaactcttt cgcttcgttt aaaatcactt tatgagaata
     6781 ttatagcagt tttggatgaa tatcaacctg aaatagcggc cgtagaaagc attttttttc
     6841 atcgtcaggt gagaagtgtt tttacactcg gtcatgccag gggtgtaata ttacttgcct
     6901 tagccatgaa agatatacca attgtagaat atagtccccg cgaaataaaa aaatctgttt
     6961 gcgggaatgg tagtgctagt aaacaacaag ttcgctatat gataaataag atttatccct
     7021 taaaagaaag tcctaaaagg gatgatgctt ttgatgcttt agcggtagcg acctgttatt
     7081 acaacaggat gaagtggctg taatgattca atatatttca ggtactctta ctttcaaaaa
     7141 tcctgtaatg gctattattg aaactatggg tattggttgg gaaataaaga ttccgattag
     7201 tacttatgaa gtgttacctt ctgttggaaa gaattgtaag ctattaactt ttttacatat
     7261 cagccaggat gatatgcgta tttttggttt ttctacagaa gcagagagag agctatttat
     7321 gatgttgaat aaagttagtg gcatagggcc gaaaatagcc ctttcaattc tttcaacttt
     7381 aagtattcct acatttataa aagcgattaa ttcaggtgaa gagggattac ttacaagagt
     7441 tcctggcatt gggaaaaaat ctgcgcaacg tttaattgta gagttgcaag atgatgttca
     7501 cactttaatg agtcatattg accagaaaga tttacaaatt ggagataaaa atagtgaagt
     7561 tgaatcagca ttgctttctt tggggttcaa tttacagcaa atcagaaaag aacttgctct
     7621 tcttgatgaa tcgatgaggc agttgaacac agaaacttta attaaagaaa ttatcaagcg
     7681 tatttatcag agaaataaat gaattttcgt accgaagcat attctactat cctaaaagtg
     7741 ctaaaagata aagagtattc tgatactcta cttcatcagc gcgcaaaaaa aataaagaat
     7801 gctggagaag atcctgcttt attttacaac ctcgtgaaag gagttatcaa gctctgtttg
     7861 aagttggatt atatcctgca gcaatatgtg gataaaaaga agtttgcttc tacagatgta
     7921 aaaattaaaa cccttcttta tatgggttta tatcagctta tgtatctaaa ttccattcca
     7981 gatcatgcag ctgtgaatga aacagttgaa ttagccaaaa ctctgtttaa tgaacaagta
     8041 gcagattttg taaatgctgt tttgagaagt tatcttcgca atcctgaaat tacctatccc
     8101 aaagaacctg aattgaggat tgcctatgaa cattcttttc ctccagaatt ggttaaacag
     8161 tggctggaat tatggggcga ggaaaatact gaatatctgg ctatgttttt taacgaaaat
     8221 cctgaactgc atatccgagt aaatacaaca gctacaacac ctgataaatt aaaagcatat
     8281 tttgctaaac gggatattat attaaaacct tctgcagcaa gctctaatat gttttataca
     8341 gctcaaacag cagaggttct ggaggatgta gctttctctg aaggttattt ttctgttcaa
     8401 gatacttctg cggcattaat tgtagaattg cttgatcctc aaaaagaaga aagtgttctt
     8461 gatttatttg ctgctcccgg aggaaaatgc agttatattg cagaaagaat gaataataca
     8521 ggagaagtta ttgctgtaga taaaatacca agtaagatga aactattaaa acaagcagct
     8581 gaccgtttgc agcttactaa tatcaaaacg gtaattacag atgctttcaa atatggtcct
     8641 gtagctcctg cttttcagag agttttagta gatgcacctt gttctggatg gggtgttttt
     8701 ggcagaaaat ctgatctacg ctggcaggtt catcagaata ttcccgagct tatcaagctg
     8761 caagaaaaag ctttggatta tgcagctaat tttgttgctc cgaacgggtt tttggtttat
     8821 tctacatgca caatgaaccc gaaggaaaac gaggaccaaa ttgcctcttt tttaaaaaga
     8881 aataaaaagt ttgaactggt tccagcttct cagtttattc ctgataagta taccagttcc
     8941 ggttttttaa aaacgatacc ttttaagcat aatatggatg gtgcatttgc cgcaaaatta
     9001 caacggatta aataagaata gatacctgca aggcaaagga aaaaccaaga ataagcaaat
     9061 cggctacctt gtaggaattg ggatttgtgt aatcattgtt actgcctttg ttttcagcca
     9121 aatctttttt cctataattt tgggaagaac acctaaagta gaagtaccct ctgtagtcgg
     9181 gatgaacctt gctcatgcta aaaaaatatt gcaggatgaa aaattgcatg ctgtagttaa
     9241 agattcttta tttagcgatg ttgcagctcc tgaggttgtt ttagaacagc atcctgtccc
     9301 tggagataaa ttacatcaag atggaacagt atatttagta attagtaaag gatcatcaac
     9361 agtcacagtt ccttctgttg ttggaaagcc ttttcaggaa gcattcataa ttttaagaaa
     9421 tcttgatttg catagcactg tagtggattc tacctattct gatatctatc ctgtaaatac
     9481 tgttattcgt agcgttcctt cagctggagc aaaagctcta aaaaaatcta cagtaaagct
     9541 cattttaagt aaaggttctg aaccaacagc tgatacgctt gactatgaaa tgataccata
     9601 ctatttttaa tagaaagaaa ttataattta ggacaagaac tatgaacgaa gagttaaaag
     9661 aaattaaaat tcgcgcccga atgatgcctg gagccgtaac ttatgatggt tttctgggaa
     9721 atgataagcg ttctctttct gaaattattg ctgctgataa taaagaactg gaacgtttta
     9781 accgttatgt tgaagaaata gcagacagaa tgaaatattt tactgatgcc acctttgatg
     9841 tgtttgataa taaggttgtt atagatgata tatatgaagt tgaaagcaca agcagtaaag
     9901 gtaatttgtc ctgcccatat ggacacggag gcttattccg taaatatgaa actactctca
     9961 ctaatcggaa gaataatatc actgttaaat ggactgcaat gaccatccat ttaattgaag
    10021 ctcatcattt ctttgggggg aagggttcac attatcgcta tgaacccgaa actcttttaa
    10081 aggccctatt taccgaacca gaaaggaaaa aataacttta taaactattc tgagtatttc
    10141 aacctcagaa ttatgagctt atgctaagag tcattacgac tattgatatc atgagtcttc
    10201 actgccgaat atttagaagt gtttacaagc tatttcggca gtgataactc ctgacttatt
    10261 gtgaggagtt atttgtgctt aaataacttt tgtatctatt aatacatttg aagcatcaaa
    10321 taacctaact gtcactc
//
LOCUS       DAORHB010000030        10272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_5801_length_10272_cov_4.981306, whole genome shotgun sequence.
ACCESSION   DAORHB010000030 DAORHB010000000
VERSION     DAORHB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5801_length_10272_cov_4.981306"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PKM80_01750"
     CDS             <1..532
                     /locus_tag="PKM80_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNV92660.1"
                     /translation="KTKDMIYLKLFWTFFKIGLFSFGGGYAILAMIRQEVVLKNAWIS
                     SSQFMDVVAISQMTPGPIAINAATFIGYQKGGLWGAVLSTLGVIFPSLIIMTLITITY
                     IKLKNQPWFKNIFQKLRLLSLGLIAAALIMVLGDAFRDLFSILVFAGSFLVSWRFKLN
                     PFYLLMGAAVIGVIFG"
     gene            complement(574..3033)
                     /locus_tag="PKM80_01755"
     CDS             complement(574..3033)
                     /locus_tag="PKM80_01755"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNV92661.1"
                     /translation="MMNDDFCTKLSQLSINGTVEEIVLRFMEHLKFDLGKDQFSAAPY
                     DCYISFASAVKDILLDRWLITQPQEYALQRKRVYYLSLEYLIGRSLTNSILNLDIQQQ
                     SIKALTEIGFDLSMLGELEWDAGLGNGGLGRLAACFLDSMASLQIPAYGYGIRYEYGI
                     FFQHIINGEQVETPDNWLRYGSVWEIPRPTRLFPVNFGGTVREIINEDGTKKKHWQPE
                     VTVMAMAYDYLIPGFQNSYVNTLRLWSAKSSREFDLRYFNEGDYVQAVAEKNNGEMIS
                     KVLYPNDNKMQGKELRLKQEYFCVCATLQDILRRFFKKNKDLRNLPDKAAIQLNDTHP
                     CIAVPELMRILVDEKNIPWETAWDITQKCLSYTNHTILPEALEKWQVQLFEKMLPRHL
                     QIIYEINHRFLSDIQISRKVDDDFLRRVSLIEEEPVKSVRMANLAIVGTHSINGVSEL
                     HTELLKHQVFSDFYSLMPDKFNNKTNGITPRRWLMLCNPLLCHLITKAIGTEWKTDLE
                     QLSDLNKYKNDSSFLEDLAEVKYQNKLDFCKYYEDMHDRRFNPDTIFDFHAKRIHEYK
                     RQLLNALGIIYLINQIRDGKELTPQSFFFAGKAAPGYFTAKLIIRFICALGDYIEKDK
                     QLSSYLNVCFLPNYRVTLAERIIPAAEISRQISLAGTEASGTGNMKFALNGALTIGTL
                     DGANIEIREAVGEDNFFLFGMTAAEVHYLKEQGYHPYEMMRQNEDIKRIFDFIESDEL
                     NPLNPGLFTPLTDLLLYQGDSYCLIADLQDFIRVNKEATETYKDKQKWNRMVLANIAN
                     MGKFSSDNTILAYAKDIWGVK"
     gene            3245..5518
                     /locus_tag="PKM80_01760"
     CDS             3245..5518
                     /locus_tag="PKM80_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNV92662.1"
                     /translation="MKLMSNYQPKLMVIIAVFYLFCFGIRAAVVQPEIALLAAKNYLQ
                     ETGLSKSVNTDDYQIFTYNSAGKLASLPHIYVQNENPVLYYIQYADGNFAVVSADDNF
                     YPVLAYSNEGLTRIDNLPSAFYYWLDTYSAQIQLIREKNALYPENSQLWQSLLEGTYT
                     NLSRTDRSVGPLLTTNWDQSWPYNALCPADQHGPGGHVYAGCVATAMGMVMKYWDHPV
                     SGVGNESYYCPGYGYQSANFETTTYLWDQMPNSISSNYLPVATLLYHCGVAVHMGYSA
                     DGSGAQSTDAAEALVDHFRYPTAQYESKDSYSNSEWNNLMTAQIDNGTPVYYSGYSNE
                     GGHAFVLDGYDTANHFHFNFGWSGSGNGYFYTTDVNGFSTWQGAIINTIPENYNINTI
                     PVVLNANNAEAGDNFTLEIKTKPVLGNWNVNHYDIMLYYDNENLDYTGYSVQGTLSQE
                     GNITIVENSPGNLSIDWNSNTSLCGGGVIIKLNFVARDAGEFLFDILSMHYNTLPVNN
                     VVFTMVNITAPVATLAESNITLSNIMHLVYNSIGFTQVNTSYLLPSWNVNHYQFNLNY
                     NPAKLEYAGYDVNGPLSDGCEINVDSSTPGVINISCDTESKFVGSGILIKPQFRAIGN
                     TSTISITQISLSDFYYNDTAVLGSGSANVVLSPYTAIDDEVISTPKPKLEIYPNPFSG
                     NTTIKFTGTAKGAVTINVYNLKGQFVKELTISDFSQREIQWNATDSKGRNVSEGIYFM
                     QWQQGNLRGTDKVLIIK"
     gene            complement(5717..6325)
                     /locus_tag="PKM80_01765"
     CDS             complement(5717..6325)
                     /locus_tag="PKM80_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV92663.1"
                     /translation="MVKIFYYLERKLGALLLRLLRKTLKFEVINQETSDRIRCIYMFW
                     HRNLLLMTLQRIDQGAAVMVSSSKDGEMIAGPLSELGYIPVRGSSSRKGSMAMREMIR
                     MSKTISLAITPDGPKGPCYTFHPGLFQIAYLAKIPIVAVAVNANKEWLFNSWDKFRFP
                     KPFAKLTMIYSDPIWVNNKEDIAIVEETIRSFLEEKETEFHK"
     gene            complement(6318..7463)
                     /gene="lpxB"
                     /locus_tag="PKM80_01770"
     CDS             complement(6318..7463)
                     /gene="lpxB"
                     /locus_tag="PKM80_01770"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNV92664.1"
                     /translation="MTRKESYKIFWLVGESSADLHSELVMKALNDKFGNLTHIGIGGP
                     RMQRQGLKPLFPFERFAVMGFVEVVKHLFFFLKVEHKLSKLFATEEIDLAILVDYPGL
                     NLRVAKMADELRIPVLYFICPQFWAWKHKRVYQLRDSVRYVACILPFEEEKLSIHNIN
                     CSYVGHPIAEEVNIELSRDAFAQFFGIDPNKKWLGFFPGSRNNEIAKMLPTFLQASEK
                     WDPNEYELLFSKSHSVNHQLYMSYIEGKKHITLIDGYTYEMMKYCDLLICTSGTVTLE
                     AAFIGTPLLICYKANFTSYLIGRHLVKIKRIGLPNIILDNDILPELIQKEMNPSNIYK
                     IGMQILNDPEKNEEIRKQLYKLKALLSDKHPSVEMPKIVRHLLDTYG"
     gene            complement(7460..8026)
                     /locus_tag="PKM80_01775"
     CDS             complement(7460..8026)
                     /locus_tag="PKM80_01775"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNV92665.1"
                     /translation="MNKYLKPLLLMILIVVLSQPLFGNSTADKTVAWLESKNVSPELI
                     VLIISMIPVIELRGSIPVAIFLFNFHWLKAALLSIIGNMIPIPFILLLVDWFFSLISK
                     VKPGKKFADWIFARTRRKGKSIENYEAIGLTIFVGIPLPGTGGWTGALAANIFGIRFW
                     RAMFCILLGVILAAIIVTILSLMGTLAV"
     gene            8656..9261
                     /locus_tag="PKM80_01780"
     CDS             8656..9261
                     /locus_tag="PKM80_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNV92666.1"
                     /translation="MNDQLQELLKKVYEEGVAKANAEAEKIINSAKEQAEDTVSQAKQ
                     KAETILKEAEKKAADLKKNTESDLNMAYNHTLTALKQKITDLILSEALNTKLNAAFNE
                     TEFVKKLIFETLTAWKENASSGTIMISEKMKPLLDDFYLKSLKDIFDGKLKVDFSPTM
                     KQGFIIAPEDGTYKIKFESDDFANLFKSYLRPRTKEILFGK"
     gene            9268..10155
                     /locus_tag="PKM80_01785"
     CDS             9268..10155
                     /locus_tag="PKM80_01785"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNV92667.1"
                     /translation="MKQEYYYFVSGLPNIIIDDTKLPWTPEEFLAEAKNQVSESDYRL
                     LEILHLPMDMDNLLRVLYKSNKEHNNEGLYEDTFWEEYLVFLRAVVENPELKTPVEFA
                     TLPSFIKEIIVKVLNQEEMQILPQTELELLKQFYAWTAESPNDFIRDWFAFDANLRNI
                     LTAINGRKFNLPYAQYLIGDSETVERLSKSHAADFGLGKDNDLFNALIRIYEQNNILY
                     RERGYDIVRWKWIDDYNFFNYFNIDRILGYYCQLRILARWIKSSPELGKEVFNSILSD
                     LNNSFSFPADFNIRSTQRK"
BASE COUNT         3297 a         1854 c         1804 g         3317 t
ORIGIN      
        1 gaaaacaaaa gatatgattt acttgaaatt gttctggacc ttcttcaaaa ttggactctt
       61 cagttttggt ggaggttatg caattttagc aatgatccgt caggaagtag ttttaaaaaa
      121 cgcctggata tcatcttcac aatttatgga tgtagtagct atttctcaaa tgactccagg
      181 ccctatagct ataaatgccg ccacttttat cggttaccaa aaagggggat tatggggagc
      241 agttcttagc actctgggag tgatttttcc ctctttgatc attatgacct taattacgat
      301 aacctatatc aagcttaaaa atcaaccctg gttcaaaaac atcttccaaa aattgcgttt
      361 actttctttg ggattaattg ctgctgctct aattatggtt ttgggagatg cttttagaga
      421 cctgttttct atcctggttt ttgctggctc ttttttggtg agctggagat ttaagctgaa
      481 tcctttttat ctgctaatgg gagctgctgt tataggggtc atttttggct aatttgtaaa
      541 cgccaaaaac acatttgtaa tttccgtggt agactatttt actccccaaa tatctttggc
      601 ataagctaag atagtattat cggaggagaa tttgcccata ttagcgatat tagctaagac
      661 cattctattc catttttgtt tatctttata ggtttctgtg gcttctttat taacccgaat
      721 aaaatcctgt aaatcagcta ttagacaata actatctccc tgatataaaa gcaaatctgt
      781 caatggagta aataaacctg gatttaaggg attgagttca tcggattcaa taaaatcaaa
      841 tatccgcttt atatcttcat tttgacgcat catttcgtaa ggatgataac cctgttcttt
      901 caaataatga acttcggcag ccgtcattcc aaataggaag aaattatctt cacccactgc
      961 ttctcgaatt tctatattag cgccatctaa agttccaatt gtaagtgctc catttagagc
     1021 aaatttcata tttcccgttc cagatgcttc tgttccagcg agggaaattt gacgggaaat
     1081 ttcagcagcg gggataattc tttctgccaa agttactcga taattgggca gaaaacatac
     1141 atttagataa gaagacagct gtttatcttt ttcgatgtaa tcacccaaag cgcaaataaa
     1201 acgaataatc aattttgctg taaagtaacc tggtgctgcc tttcctgcaa aaaagaaact
     1261 ttgtggagtt aactctttgc catcccggat ttgatttatc aagtagataa ttcccaaagc
     1321 attaagcagc tgtcttttat attcatgaat gcgttttgca tgaaaatcaa aaatagtatc
     1381 aggattaaac ctacgatcgt gcatatcttc gtaatattta caaaaatcca gcttattttg
     1441 atatttcact tctgccaggt cttccagaaa agagctgtca tttttatatt tattcaaatc
     1501 tgaaagctgc tctaaatcag ttttccattc ggtaccaata gctttagtaa ttaaatgaca
     1561 taaaagtgga ttacataaca tcagccaccg gcgaggagtt atgccattgg ttttattatt
     1621 gaatttatct ggcataagag aataaaaatc gctgaatacc tgatgcttta acagttctgt
     1681 atgcaattcc gaaactccat taatactatg ggttcctaca atagccaaat ttgccattcg
     1741 cacacttttt actggttctt cttctatcaa agagaccctg cggaggaagt catcatctac
     1801 ttttctgctt atttgtatat cagagagaaa acggtgattt atttcataga tgatttgcag
     1861 atgacgaggc aacatttttt caaacagctg aacttgccat ttttctaacg cttccggcaa
     1921 aattgtatga tttgtatacg aaaggcactt ttgggtaata tcccaagcgg tttcccaagg
     1981 tatatttttt tcatccacta aaatacgcat aagttcagga acggcaatac agggatgagt
     2041 atcattaagt tgaattgcag ctttatcagg aagattgcgt agatctttat tctttttaaa
     2101 aaacctgcgt aagatatctt gtaaagtagc acacacacaa aaatactctt gcttcaatct
     2161 aagttctttc ccttgcattt tgttatcatt gggatacagt accttggaaa tcatttctcc
     2221 attattcttt tcggctacag cttgtacata atccccttca ttaaaatatc tcagatcaaa
     2281 ttcacggctg cttttggcac tccataatct taaagtattt acatagctat tttgaaaacc
     2341 agggatcaaa taatcataag ccatagccat aaccgtcact tctggttgcc aatgtttctt
     2401 tttggtgcca tcctcattga taatttctct aacagtccct ccaaaattaa caggaaataa
     2461 tcgtgtcgga cgaggaattt cccaaacgga accatatctt aaccagttat caggtgtttc
     2521 tacttgttca ccattgataa tgtgttgaaa gaaaatacca tactcataac gaataccata
     2581 accgtaagca ggtatttgta gggaagccat agaatctaaa aaacaagctg ctaaacgtcc
     2641 taaccctcca tttcccaatc cggcatccca ttctagttca cctagcattg ataaatcaaa
     2701 accaatttca gtaagagctt taatgctttg ttgttggata tccagattga gtatggaatt
     2761 ggttaaagaa cgaccgatta aatactccag ggatagataa tagactcttt tcctttgtaa
     2821 agcatattct tgaggttgag ttatcagcca gcggtctaat aaaatatctt ttacggctga
     2881 agcaaaactg atataacaat cataaggggc agcagaaaat tggtccttac caagatcgaa
     2941 ttttaaatgt tccataaaac gaagcactat ttcttctact gtaccattaa tgctaagctg
     3001 agaaagttta gtgcaaaaat catcgttcat catatctcct cccgctaatc ataagtccat
     3061 tataaataat aaacggtttt caggataaac caaataagat gtttaatata gaaaagaagt
     3121 gtaactgcca taataattaa tgtcttccaa cttcttcatt tctatttatt ccctgctaaa
     3181 atataaattg atttggaata attcttgtat gtattatatt atctataaaa gtctaggagg
     3241 atatatgaaa ttgatgagta actatcaacc aaaactaatg gttatcattg cggtttttta
     3301 cctgttttgc tttggtataa gggctgctgt ggttcagcca gaaattgctt tgctggctgc
     3361 caagaattat cttcaggaaa cgggattatc caaatccgtt aatactgatg actatcaaat
     3421 ttttacttat aatagcgcag ggaaacttgc ttctctgcca catatatatg tacagaatga
     3481 gaatccggtt ctctattata tccaatatgc cgatggtaat tttgctgttg tatcagcaga
     3541 tgataatttc tatcctgtct tggcttattc taatgaaggt cttaccagga tagataattt
     3601 accttcagcc ttttattatt ggctggatac atattctgct caaatacaat tgattagaga
     3661 aaaaaatgct ctatatccgg aaaactctca gctttggcag agtttactgg aaggaactta
     3721 tactaattta agcagaactg atagatccgt aggtccttta cttactacta attgggatca
     3781 aagttggcca tataatgctc tatgtcctgc agatcaacac ggtcctggtg gtcatgttta
     3841 tgctggttgt gtagctactg ctatggggat ggttatgaaa tattgggatc atcctgtttc
     3901 cggagttggt aacgaaagtt actattgtcc tggctatggt tatcagagtg ctaattttga
     3961 aactaccaca tatctttggg atcagatgcc caattccata agttctaatt acttacctgt
     4021 agctacttta ttatatcatt gtggggttgc agttcatatg ggatattctg cagatggttc
     4081 aggagctcaa agtacagatg cagctgaagc acttgtcgat cattttcgtt atcctacagc
     4141 tcaatatgaa agtaaagata gctattctaa ttccgaatgg aacaatctaa tgacagctca
     4201 aattgataac gggactccgg tatattatag tggttatagc aatgagggtg ggcatgcatt
     4261 tgtcctggat ggttatgata cagcaaatca ttttcatttc aatttcggct ggagtggatc
     4321 aggaaacggt tatttttaca caacggatgt taatggattt tctacctggc agggagccat
     4381 tataaatact attccggaga actataatat caatactatt ccagtagttc ttaatgccaa
     4441 caatgctgaa gctggtgata attttacctt ggaaataaag acgaaacctg tattaggtaa
     4501 ctggaatgta aatcactatg atattatgct ttactatgat aatgaaaatt tagattacac
     4561 tggctacagt gtgcagggaa ctttatctca ggaaggtaat ataaccatag tggaaaactc
     4621 cccgggaaat ttaagtattg actggaatag caacactagt ttatgcggtg ggggagtgat
     4681 tattaaactg aatttcgttg ccagagatgc aggcgaattt ctttttgata ttttgagtat
     4741 gcactataat accctacctg taaacaatgt tgtttttact atggtaaata ttactgctcc
     4801 tgtggctact ttagcagaaa gtaacattac tctttcaaac attatgcatc ttgtttacaa
     4861 ttcaattgga tttacacaag taaatacttc ttatctgctt ccttcctgga atgttaatca
     4921 ttaccaattt aaccttaatt acaatcctgc caagcttgaa tatgcaggat acgatgtaaa
     4981 cggaccatta agcgatggct gcgaaatcaa tgtagattct tctactccag gggttataaa
     5041 tatatcctgc gatacggaaa gtaaattcgt tggctcaggt atattaatta agccccaatt
     5101 tagagctatt ggcaatacta gtactatttc cattactcaa atatctctta gtgattttta
     5161 ttataacgac acagcagttt taggttctgg gagtgcaaat gttgttcttt caccttatac
     5221 tgctattgac gatgaagtga tttcaactcc aaaacccaaa ctggaaatat atcctaatcc
     5281 tttttccgga aatactacca taaaatttac tggaacagcc aagggagcgg ttacaataaa
     5341 tgtatataac ctgaagggac aattcgtgaa ggaattaact attagcgatt tttcccaaag
     5401 ggaaattcaa tggaatgcca cagacagcaa aggcagaaat gtttcggaag gaatttactt
     5461 tatgcaatgg cagcaaggta atttacgagg cacagataaa gtattaatca tcaagtaaat
     5521 aatataaagc ttgggaagat tgaagttgtt actgctgata acagcttttc tcttcccttt
     5581 cattttttat aatgtaagat tcggaaaagc ataagcagct ttaagattaa ttcgatattc
     5641 ttgtaatatg taatggagga tatttctgag taattaggat ttccttcaag ttatatatct
     5701 tatttctgca ctttagttat ttatgaaatt ctgtttcctt ttcttctagg aaacttcgaa
     5761 ttgtttcttc tactattgca atatcctctt tattgtttac ccatatagga tcggaataaa
     5821 tcattgttaa tttagcaaag ggctttggga agcggaattt atcccaggaa ttaaacaacc
     5881 attctttatt ggcatttacg gcaactgcta caatgggaat tttagccagg taagcaatct
     5941 gaaaaagtcc gggatgaaaa gtgtagcaag gacctttagg tccatcaggt gtaatagcta
     6001 aggaaattgt tttagacatt ctaatcatct ctcgcatagc cattgatcct tttctcgagc
     6061 tggaaccgcg aactggaata taaccaagtt cggataatgg cccagcaatc atttctccat
     6121 ccttggaaga agaaaccata acagctgctc cttgatcaat tcgttgcaga gtcatcaaaa
     6181 gcaggtttct atgccagaac atatagatac agcgtatcct gtctgatgtt tcttgattaa
     6241 ttacttcgaa ttttaaggtt ttacgcagca gccgcaggag cagagcaccc aattttcgtt
     6301 ccaggtaata gaatatttta accataggta tctaatagat gacggactat ttttggcatt
     6361 tccacagaag gatgtttatc gctcaataaa gctttaagtt tatacaattg tttacggatt
     6421 tcctcatttt tttctggatc gttcagtatt tgcattccta tcttatatat attagagggg
     6481 ttcatctctt tttgaatcaa ttccggtagg atatcgttat ctaaaattat attaggtaaa
     6541 ccgatccgtt ttatttttac taaatgtctt cctatcagat aagaggtaaa attagcttta
     6601 taacagatta ataggggggt tccaataaat gcagcctcta aggttactgt tccggaagtg
     6661 cagatcaaaa gatcgcaata tttcatcatt tcataggtat agccatcaat tagtgttata
     6721 tgttttttac cttctatata gctcatatat aattgatgat ttacactatg ggatttagaa
     6781 aaaagcagtt catattcatt cggatcccac ttttcacttg cctgcagaaa agtaggtagc
     6841 atttttgcta tttcattatt cctacttcct gggaaaaatc caagccattt tttatttggg
     6901 tcaataccaa aaaattgagc gaaggcatca cggctaagtt caatattaac ttcttccgca
     6961 atcgggtggc caacataact gcaattaata ttatgaatag atagcttttc ttcttcaaaa
     7021 ggcaaaatac aggcaacata gcggacagaa tctcttaatt gatataccct tttatgtttc
     7081 catgcccaaa attgagggca gataaaatat aaaacaggaa tccgtaattc atcagccatt
     7141 ttagcaactc ttaaatttaa gccagggtaa tcaaccaaaa tagcaagatc aatttcttca
     7201 gtagcaaaaa gtttgcttag tttatgttcc actttcaaga aaaaaaacag atgctttact
     7261 acttccacaa aacccataac cgcaaagcgt tcaaagggaa agagaggttt caaaccttgc
     7321 ctttgcatcc ggggtccacc aattcctata tgcgtaagat ttccgaattt atcattcaga
     7381 gctttcatca ctaattcgga atgtagatca gcagaactct cacccaccag ccaaaagatt
     7441 ttataacttt cttttcttgt cataccgcca aagttcccat aagcgataaa atagttacaa
     7501 tgatagctgc aagaattact cctaataaaa tacagaacat tgcacgccag aatctaatac
     7561 caaaaatatt agcagctaag gcacctgtcc atcctcctgt tccaggaaga ggtataccaa
     7621 caaaaatagt taaacctatt gcttcatagt tttctattga tttgcctttg cgtctggttc
     7681 tggcaaaaat ccaatcagca aatttcttac ctggtttcac cttacttatc aaagaaaaaa
     7741 accaatctac caataacaaa ataaatggaa tgggaatcat attaccgatt atggataaaa
     7801 gcgctgcctt aagccaatga aaattaaaca agaaaatagc tacaggtata ctacctcgca
     7861 attcaataac cggaatcata gatattataa gtactatcaa ttctggtgaa acattcttag
     7921 attccaacca ggcaactgtt ttatctgctg tagaattacc aaaaagcggt tgtgacaaaa
     7981 ctactatcaa gatcatcaac agcaacggtt tgaggtattt attcaagttc actccttagc
     8041 atctttgatc tttattccat ttattattag cctatttttt gtcaatttga tttgtaacag
     8101 cgtttgagta ataaaagtaa aaagtactct ctcttattat cctttccaat catctgcagc
     8161 tattatcatt caagggcata atatcatagt ccgaatgaaa cgatgttatt atatgtgagt
     8221 tgtagctgaa taagatatta ttgttaatcc tctaaactaa aaaaaatgtt ggcaaacgaa
     8281 gatgtataaa aagcacttat gttactttta tgagaattca gaataggcag aaagaccatt
     8341 gtggtggagc atttagaaat ttcatcaaaa acgatagatt tgagcgacag ggtttacccc
     8401 ggctccaata ttatcaagat aatatccttg cgtgtaactt gttcaatggt tttattttac
     8461 actgagaacc ttgtatataa actatattct gcataatcag aaaaatctgc aggtgtatat
     8521 tttctcaggt acaccaagtt cttgggtaaa tagttagcca aaaggaatca gaataatctg
     8581 cttatcacaa tttcaattga caaaatattg gtatagtata tttgttacgc aaagtgactt
     8641 tactggagat atattatgaa tgaccagtta caagaactac tcaaaaaagt ttatgaagaa
     8701 ggcgttgcca aagccaatgc tgaagctgag aagataatca attctgctaa agaacaagct
     8761 gaagacactg tttcccaagc caaacagaaa gcggaaacaa ttttaaaaga ggcagaaaaa
     8821 aaggcagcag accttaaaaa gaacacagaa tcagatttga atatggcata taatcacact
     8881 ttaactgctc tcaaacagaa aattaccgat cttattcttt ccgaagctct caacacaaaa
     8941 ctaaatgctg cattcaatga aacagaattt gttaagaagc tcatttttga aacacttact
     9001 gcctggaagg aaaatgcctc ctctggaacg attatgatca gcgagaaaat gaaaccttta
     9061 ttagatgatt tttatcttaa atctctgaaa gatatcttcg acggtaaatt gaaggttgat
     9121 ttctcgccta ctatgaaaca gggctttatt atcgctcctg aagatggaac ttataaaatt
     9181 aaattcgaaa gcgatgattt tgccaaccta tttaagagct atttacgccc gcgcacgaag
     9241 gaaatccttt ttggcaaata ataagctatg aaacaagaat attactattt cgtctccgga
     9301 ttacccaata tcataattga cgatacaaaa ctaccttgga ctccggaaga atttttagct
     9361 gaggcaaaaa accaggttag cgaaagtgat tatcgcctcc tggaaatttt gcatctgcca
     9421 atggatatgg ataatctttt gcgggttctg tataaaagca ataaagaaca taataacgaa
     9481 ggattgtatg aggatacttt ctgggaagaa tatctcgttt tcttgcgagc tgttgttgaa
     9541 aatccggaat tgaaaactcc cgttgaattt gctacccttc cttctttcat taaagagatt
     9601 attgtaaaag tgctaaatca ggaagaaatg cagatcctac ctcaaactga attggaactt
     9661 ttaaaacaat tttatgcttg gactgctgag agcccgaatg attttatcag agactggttt
     9721 gcctttgatg caaatttgcg aaatatctta acggcaataa atggccgcaa atttaatcta
     9781 ccttatgctc aatatctgat tggcgatagt gaaacagtgg aaagactaag taaaagccat
     9841 gctgctgatt ttggtttagg taaagataat gatcttttca atgccctaat tcggatctat
     9901 gagcaaaata atattctgta tcgagaaagg ggatatgata tcgtccgctg gaaatggata
     9961 gacgattata acttcttcaa ttactttaat atagaccgaa ttttaggtta ctattgtcaa
    10021 ttgcggattt tggctcgctg gataaaatcc tcaccagaac tgggaaaaga agtttttaat
    10081 agcattttaa gcgatttgaa taatagcttc agcttccccg cggactttaa tataagaagc
    10141 actcaaagaa aatagaaggt aaataaatat gactacaggt atcgttaaag gaattatctc
    10201 taacctggtg caagtagagg tctctggacc tgtttcccaa aatgagcggt ctggcggcat
    10261 ccatgcaggg tt
//
LOCUS       DAORHB010000031        10101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_5964_length_10101_cov_6.522098, whole genome shotgun sequence.
ACCESSION   DAORHB010000031 DAORHB010000000
VERSION     DAORHB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5964_length_10101_cov_6.522098"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PKM80_01790"
     CDS             <1..761
                     /locus_tag="PKM80_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HNV92668.1"
                     /translation="ENFIPSSANAIISFLDKLIMPKVGFNIFNFVILIVSSYSLFNVI
                     RSTFDRILSMQLKTSQDTITQLVKFFGTVVFGLLIIVLLFSSSSIPIISRLLKSRVLQ
                     WIMVIVPFVLQFLAIMFLYMLLPSVRINRGALFRGAFWTTVIWVIAKSAFDFYIYNLT
                     NYQALYGVMAVLPIFLLWIYINWAIILGGMVLVSVIENKKTGGFLTKEPHNAVRITME
                     LFTNHKLNQRLEGLLSKDDLKKLVETLNEEEEDK"
     gene            758..2299
                     /gene="purH"
                     /locus_tag="PKM80_01795"
     CDS             758..2299
                     /gene="purH"
                     /locus_tag="PKM80_01795"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNV92669.1"
                     /translation="MKKYALISVSDKTGIENLAVELEKLGYTILSTSHTAEYLKKYCQ
                     QVVLVSDLTKFPEILEGRVKTLHPIIYAGILADRQNPSHEKTLSELNIDHIDVVAVNL
                     YPFAAVRKKENASEQEIIENIDIGGPALIRAAAKNYRNVTVLVDPMDYNHTLELLKEN
                     SILPKNYSAYLAKKAFSKTRDYDSEIAAYFMEMETVAKDAIPFQLSISASLNQNLRYG
                     ENPHQNAGYYTNTSAAWEVLHGKELSFNNIMDIDSALRAIRLFSEPTAIIVKHCNPCG
                     IGSGEKLAEAYTKAFATDTEAPFGGIVIVNRSLDLETATLINNIFTEIIIAPDYEPGV
                     LEFLKKKKNRRLIRYDLSLLAKPINPIEIKTLTFGYLAQNWDLVNESIENWKIVTEKQ
                     PTSEEFQALIYAWKAVSILKSNAIAIAKKDRTLGLGCGQTSRIDAVQLAIWKAKKFNH
                     ILEGSVCASDGFFPFRDCIDVLVENGISAIIQPGGSKNDEESIAACNELGITMLFTGF
                     RHFRH"
     gene            2513..2603
                     /locus_tag="PKM80_01800"
     tRNA            2513..2603
                     /locus_tag="PKM80_01800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2548..2550,aa:Ser,seq:gga)
     gene            2732..4450
                     /gene="sppA"
                     /locus_tag="PKM80_01805"
     CDS             2732..4450
                     /gene="sppA"
                     /locus_tag="PKM80_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNV92670.1"
                     /translation="MKSNKTFLWGCLIFLVAFIAACVLGFIFTFGSAVPKSAKIPTNA
                     WLYLNPSAMISDYNEIEDVKFFSVTSFSVQEINSKIIAAAKDKRIKGLLIEPKMIMTN
                     YPALGEMSLAIETFQKSGKPIVAFGENFSQADYLLASCADKIYMEPSASAGLALQGVS
                     ANMMFYKEMLDKLGIKMHILQSGAYKGAGEPYSQMELSKGTKDNIDAALQGIYNHLLT
                     FVAKNRKLDPAEVKDIFENRDDFFLTANKAKDLKLIDYAMGRDEMLSNLGLSEDNFIK
                     IGSYSPSSPKSKDSKIAVVYLNGNITPSTNYEFSSQNYISSAKVKKICKQIRDDKDVK
                     AVVLRINSPGGSALESELIYQQLIKLKKDYPLVISMGGTAASGGYYISCAGDYIIADP
                     GTLTGSIGVIGLIPETVGLGKKIGVRSQTIKYGKFAGAFNLFESYDSALIESLKRNST
                     GTYNEFQQRVMSCRNITPEQIESVAEGRIFNAEDALENNLIDEIGTLDKAINKAASLA
                     KVTTYSSTNYPKKASLMEVLKESNLLNVKEQIQISNTPELQMEKYLKQISPTGEWLYL
                     MPLKLD"
     gene            4452..5969
                     /locus_tag="PKM80_01810"
     CDS             4452..5969
                     /locus_tag="PKM80_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNV92671.1"
                     /translation="MKKDSYNTEIIVNVHPLEKRVAVLEDNRLVELFVERREQQNIVG
                     NIYKGIVKDVLPGMGAAFIEIGLERTAFLHYSDIVMDFLDIYESDQPRTKVNPEDSSQ
                     IDKLLKPGQEIVVQIHKGPIGSKGARLTGQISIPGKYLVLFPNKDKIAISRKVYSQNE
                     RNRIRNLLSEIKDPGYGLIVRTEAEGCEEDDFVTEYKALLKTWRLIEKQLKYAKAPVC
                     VFEENALENYLIRDLFGENVDRLVIDDKNFARRIISQLEDISPELISNIEIYREDSPI
                     FDAWGIEKKIETGLYSRIYLPSGGNIKIEQTEALVAIDVNTGSFTGKTHYNETIRKTN
                     LEAAAEIARQIRLRDLSGVIVVDFIDMVEEKHKTEVLDMLKKGLRRDRAKNKVYPFTE
                     LGLVEITRKRMRNTLISNFSDPCPFCNGSGRILSKNAVIMRIYRWLNRGDYFVKNKNL
                     RIMVHPDLLTHISQHSEDFVAYENQIEFAAEPSIRIDQFKVYTLPDLEEITSKFS"
     gene            complement(6429..6719)
                     /locus_tag="PKM80_01815"
     CDS             complement(6429..6719)
                     /locus_tag="PKM80_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNV92672.1"
                     /translation="MRFGQNRLRFRHWGKYKAFTCEPNQCLALDQIPEGYNATVIHNN
                     NLKTIERGLYLGAPITVFRNNIDEPNIIVAVGDARYVLDRRIARTIKARISQ"
     gene            complement(6700..8880)
                     /gene="feoB"
                     /locus_tag="PKM80_01820"
     CDS             complement(6700..8880)
                     /gene="feoB"
                     /locus_tag="PKM80_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNV92673.1"
                     /translation="MRNRPIIALAGNPNVGKTTLFNALTGSRQTVGNWPGVTVEHKSG
                     ICSFNNKVYEVIDLPGIYSLAGESPDELVARNYILSEKPDIIINIVDASNLERNLYLT
                     VQLMELGAPLIMCLSMVDIAEHNGIVIDTQHLSSHLGFAVIPLVLNKRIDVSRILEKV
                     EEYVLQPPVPDTIHYDEVVEKHLENIWQIVLKAGLEDPEKSVSGRFKKDAALNAYAIL
                     IKSRWQALKLIEGDAEINSHLTEEERSLVEKERMAIAKHRGQEPAVVIADDRYAYIRG
                     LIKDVVKRKRKSRWTFSDMVDKVVLNSYIGLPIFFIVMYLVFLVAVKLSQPCIEIINA
                     YLGWLFVEQFGKLLTLMSVPQWLIYFFSEILGGGLVTIGSFIPPIFFIYISLSILEDS
                     GYMARAAFIADKFMRKIGLPGKAFIPLIVGFGCTVPAIMATRTLEKPRDRVFASILTP
                     FISCGAKLPVYTYLVMLFFPQRADIVIFALYFLGIIMGILTGLLLKATIFKSAPGDFV
                     MELPAYHIPTINGIFMHTWHRLKSFLLRAGKTILLVIVIINILQVIQIPVGNKNEKTP
                     LLELGGKLITPVLQPMGIKNDNWPASVALISGLFAKEAIVGTLQGLYQNSEDEGAIKH
                     LTDNIKKGFGSNASVFAYLVFILLYSPCAAALTMLFKEHGFKWMLFDFGYLTVLAWMV
                     ATLVYQILAFNALSIFWFAVIGVIFIIIYINLRSIGSRYAIRAK"
     gene            complement(8870..9328)
                     /locus_tag="PKM80_01825"
     CDS             complement(8870..9328)
                     /locus_tag="PKM80_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNV92674.1"
                     /translation="MEEYADIFRKYLDKQGLKFTQPRRYILDEVFQMHTHFNAEELYD
                     RIRSKTNEISLATVYRTIPLLLEAGLVQRADRSEGRERYEHIFGHPKHIHWVCRNCGA
                     LLETDLNTLYPALAKQAKDLKFKPEDIELSVKGLCWKCNPNENENQSDEK"
BASE COUNT         3227 a         1896 c         1803 g         3175 t
ORIGIN      
        1 tcgaaaactt cattccgagc tcagcaaatg ctataataag ctttttggat aagctgatta
       61 tgcccaaagt tggctttaat atcttcaatt tcgttatttt gattgtaagc tcatattcac
      121 tgttcaatgt aatcagaagt actttcgacc gtattcttag tatgcagtta aaaacttcac
      181 aagatactat aacgcaatta gtaaaattct tcggcacagt tgttttcggg ttgttaatta
      241 tcgtgttact cttttccagt tcatccatac cgattatttc ccgtttatta aaatcccgcg
      301 ttttacagtg gataatggta attgttcctt ttgttttaca atttttagct ataatgtttt
      361 tgtatatgct actgccttca gttagaatta atcgtggtgc actatttaga ggtgcttttt
      421 ggactactgt tatctgggtg attgctaaat ctgcatttga tttttatata tataacttaa
      481 ctaattatca ggcactttat ggagttatgg cagtgctgcc aatttttctg ctatggattt
      541 atattaattg ggctattatc ttgggaggaa tggttttggt aagtgtaatt gagaataaga
      601 aaactggtgg tttcttaact aaagaacctc ataatgcagt tcgcataaca atggaactat
      661 ttacgaatca taaacttaac cagcggttgg aaggtctact aagtaaagat gatctaaaaa
      721 aactggttga aaccctgaat gaggaagaag aagataaatg aaaaaatatg cattaattag
      781 tgtttcagat aaaactggca tcgaaaattt ggctgtggaa ctggaaaaac ttggctacac
      841 tattctttcc acttcccata cggctgagta cttaaaaaaa tactgtcagc aagtagtgtt
      901 ggtttctgat ttaaccaaat ttccggaaat tctggaagga agagttaaga ccctacaccc
      961 aattatttat gccggaattt tggcagaccg gcaaaatcct tctcacgaaa aaacgctttc
     1021 tgagctgaat attgatcaca tagatgttgt agccgtaaat ttgtatcctt ttgccgccgt
     1081 tcgtaaaaaa gaaaatgcca gtgaacaaga gataattgaa aatatagata ttggcggtcc
     1141 agctttaatt agagccgcag ccaaaaatta tagaaatgtt accgttttgg ttgatcctat
     1201 ggactataat cataccttgg aactgctaaa agagaatagt attttgccaa agaactatag
     1261 tgcttatctg gcaaaaaagg catttagcaa aacacgggat tacgattctg aaattgccgc
     1321 ttattttatg gaaatggaaa ctgtagctaa agacgctatc ccttttcagt taagcatttc
     1381 tgcctcgtta aaccaaaatt tgcgttatgg tgaaaaccct catcaaaatg ccggttatta
     1441 tacaaatact agtgcagctt gggaagtttt gcatggcaag gaattatctt tcaataatat
     1501 aatggatatt gattctgctt taagagccat tcgtcttttt agtgaaccaa ctgccattat
     1561 tgtaaaacac tgtaatcctt gcggcattgg cagtggagag aaattggcag aggcatatac
     1621 aaaagctttt gcaacagata cggaagcacc ttttggggga attgtaatcg tgaatcgttc
     1681 gctggatctg gagacggcaa ccctaatcaa taacattttt accgaaatca ttattgctcc
     1741 tgattatgaa ccgggtgtgc tggaattctt gaagaaaaag aaaaaccgac gcctaattcg
     1801 ctacgacctt tctctattgg caaaacctat taaccctata gaaattaaaa cattaacttt
     1861 cggatattta gctcagaact gggatctggt aaacgaatcc atagaaaact ggaaaatagt
     1921 taccgaaaaa caacctactt cggaagaatt ccaagccctt atctatgcct ggaaagctgt
     1981 gtccatccta aaatccaacg ccattgccat cgctaaaaaa gacagaacat tagggcttgg
     2041 ttgtggacaa acatctcgca ttgatgcagt tcaattagct atctggaaag ctaaaaaatt
     2101 taaccatatt ttagagggct ccgtttgtgc ctccgatggc ttcttccctt ttcgggattg
     2161 tatagatgtt cttgttgaga acggaatttc tgctattatt caaccaggtg gttccaaaaa
     2221 cgatgaggaa agtattgctg cttgtaacga attgggcatc actatgctct ttaccggttt
     2281 ccgtcacttt aggcattaat cgtgacagag aaaggaaaaa gttgatttct cgttctactt
     2341 gaactggaaa ccacttcttt attctaaagc aaccaagttc attccacaag tatatacttc
     2401 tatctttgtt cgcatctttg tatagttata ttttagttgg gactgaaatg aaacatatat
     2461 aattctgttt gacagaaaga ggcagggaaa aaaggttgtt aaaaatacgg aaggagatgt
     2521 gtccgagttg gctgaaggag cacgattgga aatcgtgtaa acgtgtgagc gtttctaggg
     2581 ttcgaatccc tacatctccg ccattatttt actctgtaga ttaagcttaa taaagggact
     2641 tatatagcta ttgagtttat ttaatagctg ctaatttaat ttgctgtcat ttcaaatttg
     2701 tttatattat gaggtaagga gtttataaat tatgaaatca aacaaaacct ttctgtgggg
     2761 ctgtttaatc tttctggttg cttttatagc tgcttgtgtt cttggcttta tatttacctt
     2821 tggttctgca gtacccaaat ctgcaaaaat tcctaccaat gcttggttat atttaaatcc
     2881 gagtgctatg atttcggact ataatgaaat tgaagatgta aaatttttta gtgtaacctc
     2941 atttagtgtt caggaaatta actccaaaat aatagctgca gccaaggata agcgcattaa
     3001 gggtctgttg attgaaccca agatgattat gaccaattat cctgctttag gtgaaatgtc
     3061 tttagccata gaaactttcc aaaaaagtgg taaaccgatt gttgcttttg gggagaattt
     3121 ttcccaggca gattatttac tcgcatcctg cgcagataaa atttatatgg aaccttcagc
     3181 gtctgcaggt ttggctttac agggcgtttc agctaatatg atgttttaca aagagatgct
     3241 ggataaattg ggcatcaaaa tgcatatttt acaatccgga gcttataaag gagctggtga
     3301 accatattct caaatggaat taagtaaagg caccaaagat aatattgatg ctgctttaca
     3361 agggatttat aatcatttac ttacttttgt ggctaaaaac agaaaattgg atcctgctga
     3421 agtaaaagat atttttgaaa atagggacga tttttttctt actgcaaata aagcaaagga
     3481 tttaaagcta atagattacg ctatggggcg tgatgaaatg ctgagcaatt tagggcttag
     3541 tgaggataat tttatcaaga ttggctccta ttctccatct tctccaaaga gtaaagatag
     3601 caaaatagca gtagtctatc ttaatggaaa tatcacgcct tcaacaaatt atgaattcag
     3661 cagccagaat tatatcagct ctgccaaagt taaaaaaata tgtaaacaaa tccgtgacga
     3721 caaagatgtt aaggcagttg ttttaagaat aaacagtccc ggaggttctg ctttggaatc
     3781 cgaacttatc tatcagcagt taattaagct gaaaaaggac tatcctcttg ttatctcaat
     3841 gggaggaact gctgcatctg gaggatatta tatttcttgt gcaggagact atataatagc
     3901 cgatccagga actttgactg gttcaattgg agtaataggt ttaattccag aaacagtggg
     3961 gctggggaaa aaaatcggag tgcgctcaca aactataaaa tatggtaaat ttgctggtgc
     4021 cttcaatctt tttgaaagct atgattctgc cttaattgaa tccttaaaac gcaattccac
     4081 aggaacttat aatgagtttc aacaaagagt gatgagctgt agaaatataa ctcctgaaca
     4141 aattgaatct gtagctgaag gacgcatatt taacgctgaa gatgctttag agaataactt
     4201 gattgatgag atcggaactc tggataaggc aattaataaa gctgcttcct tagctaaggt
     4261 aacaacatac agttctacaa attatcccaa aaaagcaagc ttgatggaag tactcaaaga
     4321 atctaacttg ttgaatgtga aagaacagat acaaatatcc aacactcccg aattgcaaat
     4381 ggaaaaatat ctgaaacaaa tttccccaac cggcgaatgg ctatatctta tgcccctaaa
     4441 attggattaa tatgaagaaa gattcttata atacagaaat aatcgttaat gtgcatcccc
     4501 tcgaaaagcg ggttgctgtt ttagaagata atcgcttggt tgaacttttt gtggagcgta
     4561 gagagcagca aaatatcgtt ggcaacatct ataaaggtat agtaaaagat gttttgcctg
     4621 gaatgggtgc agcttttatt gaaatcggat tagagcgaac tgctttttta cattacagtg
     4681 atattgtgat ggattttctc gatatttatg aaagtgatca accccgaaca aaagttaatc
     4741 ctgaagattc ctcacaaatt gataaactct tgaaaccggg ccaagaaatc gttgttcaaa
     4801 tacacaaagg gcctatcggc tcaaaaggag ctcggttaac aggtcaaata tctattccgg
     4861 gtaaatacct cgtgcttttt cctaataaag ataaaattgc tatttcccgt aaagtatatt
     4921 ctcaaaatga acgcaatcgt atccgcaacc ttctctcgga gataaaagac cctggttatg
     4981 gtttaattgt acggacagag gcagaaggtt gtgaagaaga tgattttgtg acagaataca
     5041 aagccctgct taaaacctgg cgcttaatag aaaaacaact gaaatatgcc aaagctccag
     5101 tttgcgtgtt cgaagaaaat gctttggaaa actatctgat ccgtgatctt tttggcgaaa
     5161 atgtagatcg cttagtaatt gacgataaga actttgcccg tagaatcatc tctcaattgg
     5221 aagatatttc tcctgaattg attagcaata tagagatcta cagagaggac tcccctattt
     5281 ttgatgcctg gggaatagaa aaaaaaatag aaaccggact atatagcaga atttaccttc
     5341 ccagcggcgg gaatattaaa attgaacaaa cagaagcatt ggtagctata gatgttaata
     5401 caggtagttt tacaggaaaa actcattaca acgagacaat ccgtaaaact aatctggaag
     5461 ccgctgctga aattgctcgt caaatccgcc tgcgtgacct ttccggagtg attgttgtcg
     5521 attttatcga tatggttgaa gaaaaacata aaaccgaagt tctggatatg ctgaaaaaag
     5581 gactgcgtcg tgaccgtgcc aaaaataaag tttatccttt tacagaactt ggccttgtgg
     5641 aaattacccg taaacgtatg cgcaacactc tgatctccaa tttctccgat ccttgcccgt
     5701 tttgtaacgg tagcggaagg atcttaagta agaatgcagt aatcatgcgt atttatcgtt
     5761 ggctgaatcg cggcgattat tttgttaaaa ataaaaattt gcgaataatg gttcatcccg
     5821 atttgttaac tcacattagc caacatagcg aagattttgt ggcttatgaa aaccaaatcg
     5881 aatttgctgc agaaccttct attaggattg accaatttaa ggtttatact cttccagacc
     5941 tggaagaaat tacttctaag ttttcataat acaattttgt tacggcatcg tggagccttg
     6001 ttcacgatgc ttttttttgt tttataacat ttacagtaag tgcttatctt tcattttaat
     6061 tgcctacagc agtttagttc attagagtcc tcttctttta cacaacaatg atattcgcct
     6121 gtcaactttt taggacgcct catttccatt taggcgatcc tctaataatc cgctaaccct
     6181 tccgtaacga aactaaggaa tcgttaaaaa aaatgttaga gtgtgataaa aggatattat
     6241 ttgctgatag gataagtatg cttctttcgc ctcgttatgt ttagatattc tgttctaatt
     6301 caaaatacac ttcagctatt aaagaagccg cataaagtta taattattat cattctttat
     6361 aaagctctta gctatagcgg atagaatctg tttagaatca gaactacttt gctaaagtag
     6421 cataaactct attgacttat tcttgcttta atagtacggg caattcttct atccaaaaca
     6481 tatctggcat ctccaacagc tacaattata ttcggttcat cgatattatt tctaaatact
     6541 gttattggtg ctcccaaata cagaccccgt tcaattgttt ttaaattgtt attatgaata
     6601 actgtggcat tatatccttc tggtatttga tcaagagcaa gacattgatt aggttcacag
     6661 gtaaacgctt tatattttcc ccaatggcga aatcttaatc tattttgccc gaatcgcata
     6721 tctactccct atactgcgta aattgatgta aatgataata aaaattactc cgataactgc
     6781 aaaccaaaaa atggatagag cattaaaagc caaaatttga taaactaaag tagccaccat
     6841 ccaagccagt acagttaaat atccgaaatc aaaaagcatc catttaaaac catgctcctt
     6901 aaacagcatt gttaaagctg ctgcacaagg tgaatatagc aaaataaaaa ccagataagc
     6961 aaacacagaa gcattactgc caaaaccctt tttgatatta tccgtcaagt gcttgattgc
     7021 tccttcgtct tccgaatttt ggtagagacc ttgtaaggtt cccacaattg cctctttggc
     7081 aaaaagccca ctaattaaag ctaccgaagc aggccagtta tcatttttaa tacccattgg
     7141 ctgtaaaacc ggagtaatca gcttaccacc aagttctaaa agcggagttt tttcgttttt
     7201 gtttcccacc gggatttgaa taacttgcaa aatattaatt attacaatca ccagcaaaat
     7261 agtcttccct gctctcaaca aaaagctttt cagccgatgc caggtatgca taaaaatccc
     7321 attaatagta ggaatatgat aggcaggcaa ttccatcacg aaatctccag gcgcactctt
     7381 aaaaatagta gcctttaata ataaaccggt taaaatcccc attattatac ccaaaaaata
     7441 gagagcaaaa ataactatat ccgctcgctg cggaaaaaat aacattacca gatatgtata
     7501 aacaggtagc tttgccccac aggaaataaa tggagttaaa attgatgcaa aaacacggtc
     7561 tcttggtttt tccaaagtcc tagttgccat aattgccgga actgtgcaac caaaacctac
     7621 aatcaaagga ataaatgcct tacctggaag cccgatttta cgcataaatt tatcggcaat
     7681 aaaagctgcc cttgccatat aaccagaatc ttccagaata gataaactta tgtatataaa
     7741 gaaaataggc ggaatgaaac tgcctatggt gaccaatcca cctcccaaaa tttcactgaa
     7801 gaaatagatt agccattgcg gaactgacat caaagttaat aacttgccaa attgctctac
     7861 aaacaaccaa cccaaataag cattaataat ctctatgcag ggctggctaa gctttacagc
     7921 aaccaaaaaa accagataca tcacaataaa gaatattggt aaaccaatat aactattcag
     7981 tacaacttta tctaccatat cggaaaaagt ccaccggctt tttcttttcc gcttaactac
     8041 atcttttatt aaccctctaa tataagcata acgatcatca gcaattacaa ctgccggttc
     8101 ctggcctctg tgcttagcaa tagccattct ctctttttct acaagcgatc tttcttcctc
     8161 agttaaatga gaatttattt ccgcatcacc ttcaattaat tttagcgctt gccacctact
     8221 tttaattaaa atcgcataag catttagtgc tgcatctttc ttaaatctgc ctgaaactga
     8281 tttctccgga tcttccaaac ctgccttaag tactatctgc catatattct ccaaatgttt
     8341 ttccactacc tcatcgtagt gaatcgtatc cggaactggt ggctgcaaaa catattcctc
     8401 tactttttcc aatattcggg aaacatcaat acgcttattc aaaaccaaag gaataactgc
     8461 aaaacccaaa tgcgaactta aatgctgtgt atctatcaca attccattat gctctgcaat
     8521 atctaccata gataaacaca taatgagcgg tgcccctaac tccatcaatt gaactgttaa
     8581 atataaattc cgttcaagat tggaagcatc tacaatgtta ataattatat ccggtttttc
     8641 agacaaaatg taattacgag ctacaagttc atccggactt tcccctgcca gagaatagat
     8701 cccgggaaga tctataactt cataaacctt attattaaag ctgcatatcc cagatttatg
     8761 ctctactgtt actcccggcc aattgccaac cgtttgtctg gaaccagtta aggcattgaa
     8821 aagcgtggtt ttgccaacat ttggatttcc agcaagtgcg attataggtc tatttctcat
     8881 ctgattgatt ctcattctca tttggattac acttccaaca taatcccttt acgctcaatt
     8941 ctatatcttc gggcttaaac tttaaatctt ttgcctgttt agctagcgca ggataaagcg
     9001 tgttcaaatc cgtctctaat aaagcaccac aatttctgca gacccagtga atatgttttg
     9061 ggtgaccaaa aatatgctca taacgctctc tgccttcact acgatccgct ctctgtacta
     9121 accccgcttc cagcaataaa ggaattgttc tgtaaactgt cgccaaagat atttcgttgg
     9181 tcttgctcct aattctgtca taaagttctt cagcattaaa atgagtatgc atttgaaaga
     9241 cctcatccag aatataacga cgcggctgag tgaacttaag tccttgctta tcaaggtatt
     9301 ttctaaatat atcagcatat tcttccatta ttttgtctcc tactatattg agaatcattt
     9361 acaatatgat tcagatgccc ttatttgtca agtgcgattt tttcccctcc gctaagttgt
     9421 ttattttata tctgttctct ttatacatcg agagcttttc taatgaatat caaaattatc
     9481 gatacatcgt gattatggat gaagctatct gtttttgact ttaaccgaac ttcagctttg
     9541 aaagtatcag gtaatgaaat acttttcctg ttatatcaac ttctcgttgc aaacatttca
     9601 gatgggttgg gttggaattg ttcgttattt gtggcttcga agtgattttc tgttttaact
     9661 ctaaaccgag cttcagcttt gtaagttgaa ggcaaagaac aaattttcct gttgcctctg
     9721 cttctcgttg caaattcaag aaggatcggt ttagagtcaa aacttccacc gatgcttgtt
     9781 ttgacttaca attttgccac taccctctct gtaataacat acctaaaatt ataaacattt
     9841 ccatccgagc tataactggt ctcccaactt caaaattgtg agttaaaaca agaaaagagc
     9901 acactggcag atgagttaca gctttgaaag tatcaggtaa tgaaatactt ttcctgttat
     9961 gtcaacttct cgttgcaaac atttcagatg ggttgggttg gaattgttcg ttatttgtgg
    10021 cttcgaagtg attttctgtt ttaactctaa accgagcttc agctttgtaa gttgaaggca
    10081 aagaacaaat tttcctgttg c
//
LOCUS       DAORHB010000032         9831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_6235_length_9831_cov_5.040200, whole genome shotgun sequence.
ACCESSION   DAORHB010000032 DAORHB010000000
VERSION     DAORHB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9831
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6235_length_9831_cov_5.040200"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKM80_01830"
     CDS             complement(<1..667)
                     /locus_tag="PKM80_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV92675.1"
                     /translation="MLKQVTIKGFKGITDLTLNLDKINVLIGINSSGKTTILQALDLL
                     ANCVSRDVSEYLKDKNWKVSDIKSQISKSSLLSYNALFEFEENGNPFLLIWQIEFKLI
                     SATSVNLTKESILKVNGKWNKAYCNIDKQQKLFENAIPLYTYRDGIVIYEAFHDQKAK
                     NQESEFYLSLGSSGLKIIDFAGNKDICLLKEFLKHTTNFETLAAENMRRSSRGTTEDI
                     GIGG"
     gene            complement(758..1687)
                     /gene="rnhC"
                     /locus_tag="PKM80_01835"
     CDS             complement(758..1687)
                     /gene="rnhC"
                     /locus_tag="PKM80_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNV92676.1"
                     /translation="MHKEIEIYLSHLLPELQKAGISIDEQQEIDYGVQLRVSRHNYKS
                     ILNIYYSQKKGISTIPMGLNNPLKKLLSDLCSFQEELDIKMPPMHNWNAWIGSDECGK
                     GDYFGPLIVCAFAFDKKQEKEFKNIGVMDSKKLKTDAIYRIAKHLYKNYANCINCIVL
                     KPEKYNELIAKFKLQRKSLDDLLAWLHSTAIINVQKNHPESEGALVDQFSISQKVWRS
                     LKEKKFPLPCIERTGAEVDPAVAAASIIARYQFLESFAAMREFYQLNFPLGANGTVIK
                     TAREYINKYGEKRLGEVAKLHFKTTNQIENLSE"
     gene            complement(1687..2961)
                     /locus_tag="PKM80_01840"
     CDS             complement(1687..2961)
                     /locus_tag="PKM80_01840"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNV92677.1"
                     /translation="MLYKEFLDHIYKRYSGNVKLELNRMTGLLSDMEDPERYLNGFHI
                     GGTNGKGSVCATLEALCLASGLKTALNTSPHLINYSERFRINGSELPFTTILSTFRRY
                     ETLFKKWDASFFEITTALAFAMFSKQNVEVAIIEVGLGGRLDATNLFTPDISVITNIG
                     LDHIKTLGGTLEIIAAEKAGIIKKGIPLIIGDMEESPRSIVEGVAMAKQAPVFRFGRD
                     WNTETLSDSLDGISFNYSFGKYEFENLQANLIGEHQVVNVGTALTAFLLYAEKRDIPI
                     SEEIIRSALKQINWKGRLQVLSQKPLVITDGAHNVHGAKSLLRTLDRLFPNRKLKFLV
                     SILRDKDYPDMISLFCSKAEFIYITKNQSERAASIEEQVEEVKKHNVPYKTVPTVAEA
                     YALAVKECKPDDILITCGSLYTVGEILAISMN"
     gene            complement(3076..3720)
                     /locus_tag="PKM80_01845"
     CDS             complement(3076..3720)
                     /locus_tag="PKM80_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92678.1"
                     /translation="MLRNQNGISFYTVLSIILFIALVFILALPNFFNLDKEKNQEDCI
                     NNMKQIWVATTDYMRDTNTDFNGDLSLLTKTPKAQDPKHTYLSSNLYCPETSRQKNDY
                     TVYGKYVAEQIGTEIKHNYGVIVYCPNLVKFPKHIIPKAFYENMDPTQLQNYIIDDID
                     YIDTETGSNGAKKVELIEKYIQIWKTDPDAFVKRKANTTALRAILFPDKFSVSQ"
     gene            complement(3776..4297)
                     /locus_tag="PKM80_01850"
     CDS             complement(3776..4297)
                     /locus_tag="PKM80_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV92679.1"
                     /translation="MKHKKRKKDNTRRIISIFLVLFLLLILVYNPISLRLTTIGVAIY
                     YGLNPVIFYRLIKTESSFRSFAISDAQAIGLGQIKESTAFYIHKEHKRGYLFLPLYNL
                     KLAAKYLNYLKKRYNDNWSLTLAAYNWGESNVNRRISSHQIEKDKDYRHLFSDIPETY
                     NYINKILYPKNKT"
     gene            complement(4391..4876)
                     /locus_tag="PKM80_01855"
     CDS             complement(4391..4876)
                     /locus_tag="PKM80_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNV92680.1"
                     /translation="MAVYSVNEVIEMAVQIERNGYAFYNEAIKRSDLDEASKELLTYL
                     RDEELTHENTFLALRDEIDLEVLKLSIDWELIAEYLKAIVDSRIFNSESSALQLVANS
                     KDAREIINNAITFEKDTLLYFHTLSDNISDPKTHNALRKIINEEIRHILKLNEFKKKM
                     G"
     gene            complement(5066..6076)
                     /locus_tag="PKM80_01860"
     CDS             complement(5066..6076)
                     /locus_tag="PKM80_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNV92681.1"
                     /translation="MNKDNYLLAKAFLPKQRLWQACGIGCDYLLSLAIHKPVVNFYPP
                     ALMLEPTNICNLRCPLCLTGNGTLNRPKGMMSLALFKKICKDVQDKIGMLILWNQGEP
                     FINPDFYEMLKIASKHQFYTMTSTNASLELDIEWIVKSGLNKIIISMDGITEETYNNY
                     RVNGDFELVLKNMQALVRCKEKLHSKTPYLVWQFIIMKHNENELEKVKHLAKELKVDK
                     LEFKTAQIYNGEDQAFLPNNSKYSRYQKNGDNFILKTKLKNRCRRLWTQPVINWNGEM
                     NICCYDKNGTIQIGNMEKNSFSELWFGKAMQNMRKAILKDRSAFEICRNCGEGIVQKI
                     KD"
     gene            6748..7860
                     /locus_tag="PKM80_01865"
     CDS             6748..7860
                     /locus_tag="PKM80_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92682.1"
                     /translation="MKVKVLFMLTLLIASLCAAIDFTYVDMENYGSGVSRIVVGFYGT
                     GNYEVIPNSDYVEVKIDNNNPSKVNISYEGGNIVRSIVQRNNSIRISVSPTFRLERMV
                     LEDPFRLVLDIIVAKPNRQQKLDIAEFYTSCGKLNSADKIYVELNRDYPNDGEVLYKW
                     ALLLEQRGSNRLKAIVAKIPKNSAYYSAGQDILNRVYGRKAAAGTSSEKAALQEKPKS
                     VEEPKPIEPLKANKEIPPAVETEQTVQSESLVATPPLPQDIQEAPLPSGSEESKLSFF
                     EKNPLLIVFFVLLLLLIIIIFIFIHSGKKKPKPVVFAPPENRKEGEALDTETLCRMVS
                     RLLINGWTNKQIAKELKLPEEEVERLVKLSHQGNYI"
     gene            7864..9390
                     /locus_tag="PKM80_01870"
     CDS             7864..9390
                     /locus_tag="PKM80_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92683.1"
                     /translation="MPAKICVIVLLLWCSLLPAMRFIPNSAFNPEVQNSLKPSLLSYP
                     QSAKDDSISTYGNGLHIPLVPIPRFTDAIMDFFRLGKANHSLMSYQSNDRKFKADMNV
                     TAGLEPVKKDDICSFLYGGIYLESQYGEHFELNTNWWNGIFLGDQKTAETSWLVDGYY
                     NYSNNKLNIDNLSGDISYNANNLTLALGRGKFPVGNAISGSIILNDEVNDYGYILAEA
                     RSGLFSISFMHASLMADSTYNIYNNPYYNSKNYPDKYLALHQFGFQPYSNLNFFLGEE
                     IIYGNRSLDINYFLPVAFWRAIEHNLWDRDNVMIYAGGSYRPIQSLFFYTQVLIDEFS
                     FSELGSDWWGNKYALQGGIKWKTPFALPESPFADITTELTAIRPFTYTHYLNHTMFST
                     DGKPLGYPKGSNIADFSCLLRLPYRNILLWQGSVSYSKQGSFGSDWSEDYHNAFPDSE
                     AMDNGTASWFQGTKTNTITLKSNVLIDVFAHHRLLFGWEGCHNDDWQNTLYSAWQLIF
                     "
     gene            9401..9814
                     /locus_tag="PKM80_01875"
     CDS             9401..9814
                     /locus_tag="PKM80_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92684.1"
                     /translation="MHKVDRLEFPKRYKLKQVVLVVVIILVIWGIFIQRSGLKKTASE
                     IQISDISISRYGTQFIELEYTLANTGKKDQEIALMAKVWDANGEELASALYSIKIKAN
                     SKSRRSKMLDKLNRALKEGERPYKAEISLYTRHIP"
BASE COUNT         3063 a         1748 c         1722 g         3298 t
ORIGIN      
        1 ttcccccgat accgatatcc tctgtagttc ctctacttga tctacgcata ttttctgctg
       61 ctagagtttc aaaatttgtt gtatgtttaa ggaattcttt cagcaggcaa atatctttat
      121 taccagcgaa atcaataatt tttagccccg aagaacccaa ggacaaataa aattctgatt
      181 cttgattttt tgctttctga tcatgaaatg cttcataaat cacaatccca tctctatagg
      241 tgtataaagg aatagcgttt tcaaaaagct tttgctgttt atctatgtta caatatgctt
      301 tattccattt gccatttacc tttaaaatgc tttccttagt tagattaaca ctggtagcag
      361 aaatgagctt aaattctatc tgccagataa gaagaaaggg gttcccgttt tcttcaaact
      421 caaaaagtgc attataggaa agcaatgaac ttttggatat ttgggattta atatcggaga
      481 ccttccaatt tttatccttt aaatattccg atacatctcg ggaaacgcaa tttgccaata
      541 aatccaatgc ttgcagaata gtggtttttc cggaagagtt gataccaatt aaaacattta
      601 tcttatcaag gtttaaggtg agatcggtaa tacctttaaa gccctttatt gttacttgtt
      661 tgagcatctc tacctccaaa ggcagttaat taattaatct ttaagcaatg atgttatatt
      721 ctctgatagt gtcaaggtat aattcgtaat ctatatatca ttcagataag ttttctattt
      781 ggtttgtggt tttaaaatgc agtttggcaa cttcgcccaa gcgtttttcc ccatatttgt
      841 taatatattc ccgcgcggtt ttaataacgg ttccatttgc tcccaagggg aaatttagct
      901 gatagaattc acgcattgca gcaaaacttt ccaaaaattg atagcgagca ataatagaag
      961 cagcagccac cgcaggatca acttccgcgc cggttctttc tatacaagga agaggaaact
     1021 ttttttcttt tagggatctc cacacttttt gagaaatgct gaattgatcc accagcgcac
     1081 cttcactttc cggatggttt ttttgcacat ttataatagc tgtagaatga agccaggcaa
     1141 gcaaatcatc caacgatttt ctttgtagtt tgaatttagc aatcagttca ttatattttt
     1201 ccggttttaa aactatgcaa ttgatgcaat tggcataatt tttgtagagg tgtttggcga
     1261 ttctatagat agcgtcggtt tttagctttt tgctatccat cacgccgata tttttaaatt
     1321 ccttttcctg ttttttgtca aaggcaaagg cacacacaat cagcggaccg aaataatcgc
     1381 ctttaccaca ttcatcacta ccgatccagg cattccaatt atgcatcgga ggcattttaa
     1441 tatccagttc ttcctgaaaa ctacacaagt ccgatagcaa tttttttagc ggattattca
     1501 accccatagg gatagtagaa attccctttt tttgtgaata gtatatgttt aggatgcttt
     1561 tataattgtg gcgggatact cttaactgca ctccataatc aatttcttgc tgttcatcta
     1621 tggaaatacc agctttttgc agttccggaa gcaggtggga aaggtatatt tcaatttctt
     1681 tatgcatcaa ttcatagaaa tagccagaat ttctccaaca gtatataaag aaccgcaagt
     1741 aattaaaata tcatctggtt tgcattcttt tacagccaga gcataagctt cggcaactgt
     1801 tggaactgtt ttatagggga cattgtgttt ttttacttct tccacctgtt cttcaatact
     1861 agctgcgcgt tccgattggt ttttagtaat atagataaat tcagctttgc tacagaatag
     1921 ggaaatcata tcaggataat ctttatctcg caaaatagaa acaaggaatt ttaacttacg
     1981 gtttgggaat aacctatcca aagtccgcag cagagattta gcgccatgta cattatgggc
     2041 tccatcagtt attactaacg gtttttgaga aagaacctgc aacctacctt tccagtttat
     2101 ctgttttaat gccgagcgaa ttatctcttc agaaataggt atatcccttt tttcagcata
     2161 taagagaaaa gcggttaaag cagttccaac atttactact tgatgttctc cgattaaatt
     2221 tgcctgtaga ttctcaaatt cgtatttgcc aaaactatag ttaaagctaa ttccgtccaa
     2281 agaatccgac agtgtttcag tgttccaatc gcgtccaaaa cgaaagacag gtgcctgttt
     2341 tgccattgcc acaccttcaa ctatagatcg aggagattct tccatatcac ctattatcag
     2401 aggtatccct ttttttatta ttcctgcttt ttcagccgca ataatttcca gagttccgcc
     2461 aagagtcttt atatggtcta acccaatatt agtaattacc gaaatatcgg gagtaaagag
     2521 attggtagca tccaatctgc ctcctaagcc aacttcaata atagctacct ctacattttg
     2581 tttactaaac attgcaaaag caagggcagt tgttatttcg aagaacgaag catcccattt
     2641 tttaaacaag gtctcataac gacggaaggt gcttaaaata gttgtaaaag gtagttcact
     2701 accattaatt ctaaatcttt cgctatagtt gatcagatgg ggagaagtat taagtgcagt
     2761 tttcaatcca cttgctaagc agagtgcttc caaagttgca caaacacttc ctttcccatt
     2821 agtgcctccg atatgaaaac catttaaata gcgttcggga tcttccatat cagaaagcaa
     2881 acctgtcatt ctgttcaatt ccaattttac attaccagaa tagcgtttat agatatgatc
     2941 aagaaattct ttatagagca tttggttctt ctcatttttt atatttttat gctgcttact
     3001 acaaaaacag ttcaaacggc atcacgatta atgatgctgt ttgaactaac cgaaaaatag
     3061 cttttagata atgcgttatt gactaacgct gaatttatca ggaaaaagaa tagctcttaa
     3121 agcagttgta ttcgctttcc ttttgacaaa agcatcggga tcagttttcc aaatttgtat
     3181 atatttttct atcaattcaa ctttttttgc cccatttgag ccggtttcag tatcaatata
     3241 atcgatatca tcaatgatat aattctgtag ctgagtggga tccatatttt catagaaagc
     3301 tttgggaatt atgtgttttg ggaatttcac aaggttgggg caatatacga taacaccata
     3361 gttgtgtttt atttcggtac caatttgttc agcgacatat tttccgtaaa cagtataatc
     3421 atttttttgg cgactggttt cggggcaata caaattagaa gaaagataag tatgttttgg
     3481 atcttgtgct ttaggggttt tggtaagtaa agaaagatca ccattaaaat ctgtatttgt
     3541 atcacgcata taatcagtag ttgctaccca gatttgtttc atattattga tacaatcttc
     3601 ctgatttttt tctttatcca gattaaagaa atttggcaat gccaagataa acaccaaggc
     3661 aatgaatagg attatactta agacagtata aaacgagatt ccgttttgat tacgcagcat
     3721 aaatcactcc tgaaatgaat tatagaaata tgatttttat caagggctat tctcgtcaag
     3781 ttttattttt aggatagagg attttattaa tgtaattata ggtctcaggt atatctgaaa
     3841 agaggtgtcg atagtcctta tctttttcta tctgatgaga actaatccga cgatttacat
     3901 tgctttcacc ccaattataa gccgccaaag ttaagctcca gttatcatta tatctctttt
     3961 ttaaataatt aagatactta gctgccaact tcagattgta taacggtaaa aacagataac
     4021 ctcttttatg ttccttatga atgtaaaaag cggtggattc tttaatttga ccaagaccaa
     4081 tagcttgagc atcggagatg gcaaaactac gaaacgaact ttcagtttta attaatcggt
     4141 aaaaaattac tggattcaga ccataataaa tagctacgcc gatagtagtc aacctcaaag
     4201 agattggatt ataaaccaga atcaataaca gaaacaacac cagaaagata ctgataatcc
     4261 ttctcgtatt gtcttttttc ctttttttat gtttcacttg ctatctatac tgcttatctg
     4321 gctacatttc tgtttcgccg caatccattc ctgtattttt aatgttaata taggttttgt
     4381 taataggtag ttatcccatt tttttcttaa attcgttcaa tttcaaaatg tgtctgattt
     4441 cttcgttaat tatttttcgt aaagcattat gggttttggg atcactgata ttatcggata
     4501 gagtatgaaa atagagaagt gtatcctttt cgaaagtgat tgcattatta attatctctc
     4561 ttgcatcttt tgaatttgca acaagttgca gagcagagga ttcgctgtta aaaatacggc
     4621 tatcaacaat agcttttaag tattcagcaa ttaattccca atcgatagat agtttcaata
     4681 cttctaaatc gatttcatct cgcaaagcca aaaaagtgtt ttcatgcgtt agttcttcat
     4741 cccgcaaata ggttaataac tctttggagg cctcatcaag gtcactgcgt tttatggctt
     4801 cattataaaa tgcatagcca tttctttcaa tctgcaccgc catttcgatt acttcattaa
     4861 cagaatatac tgccattttt tcctcctatg gaatacgatt tttttataat atatctcgct
     4921 atgaaaaaaa gtcaaggaaa aagtgtaaaa gcaccttgga aggataggat attacagaaa
     4981 tagagattta aatacagagt ataattttct acctacgcta aatgtagttc caaaactgaa
     5041 attatgtttc aagaaagcca ataagctaat ctttaatttt ctgcactatt ccttctccgc
     5101 agtttctgca aatttcaaaa gcggaacgat ctttcaatat cgcctttcgc atattttgca
     5161 ttgcctttcc aaaccataat tccgaaaaac tgttcttttc catattgcca atttggatag
     5221 ttccattttt atcgtagcaa caaatattca tttcaccgtt ccaatttata actggttgag
     5281 tccataaccg cctacagcga tttttcagct tagttttcag gatgaaatta tctccgtttt
     5341 tctgatagcg actatatttc gagttattgg gcagaaaggc ttggtcttcg ccattgtata
     5401 tttgggcagt tttaaattcc aatttatcta ctttcagttc ttttgccaga tgtttcactt
     5461 tctccagctc attttcattg tgtttcataa ttataaattg ccaaacaaga tagggtgttt
     5521 tactatgcaa tttttcttta cagcgaacca atgcctgcat atttttgagg acaagttcaa
     5581 aatctccatt tacgcgatag ttattatatg tttcttccgt tattccatcc atagaaataa
     5641 taattttatt tagtccgctt tttactatcc attcaatatc cagttccaaa ctggcattag
     5701 tagatgtcat cgtataaaat tgatgttttg aggcaatctt tagcatttca taaaaatcag
     5761 gatttataaa gggctcgccc tgattccaca aaatcagcat tccaatttta tcctgaacat
     5821 ctttacagat ttttttaaaa agtgccaaag acatcattcc tttgggacga ttcaatgtcc
     5881 cattcccagt taaacacaaa ggacagcgta aattacaaat attcgttggc tccaacatta
     5941 aagcaggcgg gtaaaaatta accacaggtt tatgaatagc aagcgacaaa agatagtcac
     6001 atcctattcc acaggcctgc cataaacgct gttttggcaa aaaagccttc gctagtaaat
     6061 aattatcttt attcatattt ttgctttttt catatatggc ttttgcgtca agagaaaaga
     6121 gggaactcag aatataaatg tttttgtaat ataacaatta tgcccaaaaa cagatagaaa
     6181 agtgcttaga tcacagagtt taaagcgaag agcattgaat ttatttactg ttttttctta
     6241 cttcttgctt aataagctgc ggtagcgatg cggtgctgta cctaggtatt ttccccatca
     6301 gcatcgccac aaccccacgc tagcttataa gcaagaaata gcccagcttc gtaccctcaa
     6361 gcattattta tgcccctatt aacatttggc agaatagcat tttagccgga aaaacagatt
     6421 gatgcttacc ctcctttttt ctttattttg ttatgtttgc tttattagtg ttaaaaatag
     6481 cttgccaatt ttaaagcggc aaaaattata gtaaaaatgg aatagttcag acaagataga
     6541 gctatataaa agggaagata ttagctgata aggtgttaac aatatggaac ttagatgaaa
     6601 agaggaagtg tattgttgca taggttttat attttatctt ctatataggc ttagaattgg
     6661 gattgattta tacaagagga ataagagtgt aagtgagatg attgttgata tcaggttttt
     6721 ttcaaatata gagaaggaga cagctaaatg aaagttaaag ttttatttat gcttacctta
     6781 ttgatagcgt cgctttgtgc agctattgat tttacttatg tagatatgga aaattatggt
     6841 tcgggggttt cgagaatcgt tgtaggattt tatggcacag gtaattatga ggttataccc
     6901 aatagcgatt atgtggaagt gaagatagat aacaataatc cctcaaaagt aaatatcagc
     6961 tatgaaggtg gtaatatcgt ccgaagcatt gtgcaaagaa acaatagtat tcgaatttct
     7021 gtttctccta catttcgatt ggaaaggatg gttttggaag atccttttcg tttagttttg
     7081 gatatcattg ttgctaagcc caatcgtcag caaaagcttg atatagcaga attttacact
     7141 tcctgtggaa agctgaatag tgctgataaa atttatgtgg agctaaaccg tgattatccc
     7201 aatgatggtg aagttttgta taaatgggca ttattattgg aacaaagagg cagcaatcgc
     7261 ttgaaagcta tagtagcaaa aatacccaaa aactcagctt attattctgc aggacaggat
     7321 attttgaata gggtgtatgg aagaaaagca gctgccggga cctcttcaga aaaagctgca
     7381 ctccaagaaa aacctaaatc agttgaagaa cctaagccaa ttgagcccct aaaagctaat
     7441 aaggaaatcc cccctgctgt agaaacagaa caaactgtgc agtcagaatc tttagttgcc
     7501 actcctccct tgccacaaga tatacaggaa gctcctcttc cttcaggatc agaagaaagc
     7561 aaattatcct tttttgaaaa gaatcccttg ctaatagtgt tttttgtgtt acttctgcta
     7621 ttaattatca ttatttttat ttttatccat agtggcaaaa agaaacccaa acctgttgtg
     7681 tttgctcctc ccgaaaatag aaaagaaggc gaagctctgg atacagaaac tctatgtcgg
     7741 atggtaagtc gtttattaat taacggttgg acaaataaac aaattgccaa agaactaaaa
     7801 cttccggaag aagaagtaga acgcttagta aagcttagtc atcaaggaaa ttacatctaa
     7861 aatatgcctg ccaaaatttg tgttatagtg ttattactct ggtgttcatt gttacctgct
     7921 atgcgtttta ttcctaattc tgcttttaat cctgaagtac aaaacagttt gaaaccgagt
     7981 ttacttagct atcctcaatc cgcaaaagat gatagtatct ctacctatgg taatggatta
     8041 catatacctc ttgtccctat tcctcgtttt actgatgcca taatggattt ttttcgctta
     8101 ggaaaagcaa accattctct gatgagttat caaagcaacg atcgtaaatt taaggcggat
     8161 atgaatgtta ctgcaggttt ggagccagtg aaaaaagatg atatctgcag ctttctttat
     8221 ggtggtatat atttagaatc gcaatatgga gaacactttg agctgaatac taactggtgg
     8281 aatggcattt ttttgggtga tcaaaaaact gcagaaactt cctggctggt tgatggctac
     8341 tataattata gcaataacaa actgaatata gataatctta gcggagatat cagctacaat
     8401 gccaataatt taaccctggc tttagggcgg gggaaattcc ctgtgggcaa tgcaattagc
     8461 ggtagtataa ttctaaatga cgaagtgaat gattacggct atatattagc agaagctaga
     8521 tctggattgt tcagcatttc ttttatgcat gcatctttaa tggcagatag cacttataac
     8581 atatataaca atccttatta caacagcaaa aactatcccg ataagtattt agcgctgcat
     8641 caatttggct ttcagcccta ctcaaatttg aactttttct taggtgaaga aattatatac
     8701 ggaaaccgct cgctggatat taattatttt ttgcctgttg ccttttggcg cgcaattgaa
     8761 cataatcttt gggatagaga taatgtaatg atttatgcag gaggttcata tcgtcctatt
     8821 cagtcgcttt tcttttatac tcaagttctt atagatgaat ttagtttcag tgaactgggt
     8881 agtgactggt ggggaaataa atatgcgctg caaggaggaa taaaatggaa aactcccttt
     8941 gctttaccag aaagtccttt tgcagatata actacagaac taactgctat ccgtcccttc
     9001 acttatactc actatttaaa tcatacaatg ttctctactg atggtaaacc tcttggctat
     9061 ccgaaaggtt caaacattgc agatttttcc tgtttactac gcttaccata tagaaatatt
     9121 ctgctttggc agggaagtgt atcttatagc aaacagggaa gttttggcag tgactggagt
     9181 gaagattatc ataatgcttt tccagattca gaagcaatgg ataatggaac ggcttcctgg
     9241 tttcaaggaa caaaaactaa tactatcacc ttaaaaagta atgtgcttat agatgttttt
     9301 gcacatcacc gtttgctttt tggttgggaa ggctgtcaca acgatgattg gcaaaatact
     9361 ctatattccg cttggcaatt aatcttttag gacaaatatt atgcataaag tagaccgtct
     9421 ggaatttcca aaacgctata aactgaaaca agttgttttg gttgtagtta ttatcttagt
     9481 tatttggggc atttttatcc aacgctcggg tttaaaaaaa acggcttcag aaatacagat
     9541 aagtgatatc agcatcagcc gttatggcac tcaattcatc gagctggaat acacccttgc
     9601 caataccggc aaaaaagatc aagaaattgc tttaatggca aaagtttggg atgccaatgg
     9661 tgaagaactt gccagtgctc tttatagcat taaaattaag gcaaatagta aaagtagaag
     9721 atcaaaaatg ttggataaat tgaaccgagc tttaaaagaa ggggaaagac cctataaagc
     9781 agagatatca ttatatacca gacatatacc ataaaagagt gcgggagttc a
//
LOCUS       DAORHB010000033         9510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_6585_length_9510_cov_5.989847, whole genome shotgun sequence.
ACCESSION   DAORHB010000033 DAORHB010000000
VERSION     DAORHB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_9510_cov_5.989847"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..2468
                     /locus_tag="PKM80_01880"
     CDS             87..2468
                     /locus_tag="PKM80_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV92685.1"
                     /translation="MKCRVIIICLLCLIGLFACEVNTMYNARNYFKSAQSKPLNSNGR
                     PNAQAIDEYTKAIKKCGKIIATDKKGKRVEEAYYLMAKSLYYKGNSAFQAKDQFQNLV
                     IRFPDSKYVPEAYIYIAKILRETNQPKEAEKLLDEFLRNPKYRKHHPMALSVLADFAI
                     QDKDYIKAQHYLERIINEFPKTKEYREAYFLFGKNYYEQKDFAKSLEAFKKMQNARGI
                     DKAKKLDGTYYIGLNELELGEAEKALKTAKGLLKAESRPDKLPFVRLLKARAQFALGD
                     TTAARSEIELINKNYPRTAGSAGGYYYLAEHYYYNQGDIHKAIASYNRVRTEFSSSPL
                     AASAQVKSSALGLVSPRQGLDCEKNLSNFLDYQYQAAESFLNVLALPDSAIARYRIVI
                     AQKDILVNRCDSLLALISKNNAILDSLRAIVPIQEISEEDSIGIVDKTELLTQLDDLL
                     DVSTFTDSLSSTADSLKITKPEIAAEDSLLKDNIIAIQDSSSNQIDTLVVSIRSEITR
                     LEQENELCQDRINTLEEIIERFDSEIIPFCLFAIGSIYHNNYPESPENAEILTQMQEQ
                     YPENKFTKALYALQNDLPVRLIDPLEEREEQLLELYLEQIYTAPDSALVGLEELTRSR
                     YPKIKLAANYRLGWFYSFEQVDTLLAKPYLNAVLEEPEAGEYATITQRFFNGDIFLLR
                     GAPKFDIPVADSTVVDSTSLNINENKKELPLPEGAEIHKSLSDSLKIYNEIPVLVDTL
                     AKIIPPVENKQEEDISSLETETPEVNTPEPEENPVPTLPEESVPEKKEEIPLE"
     gene            2465..3103
                     /locus_tag="PKM80_01885"
     CDS             2465..3103
                     /locus_tag="PKM80_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92686.1"
                     /translation="MKFISYPISGQHLYLKLAVFLTGLAIGVDSSLRQLLIQLILILV
                     ILGFEPSLYRLFLNSLKRLLPFFAGYWVFATLFAQDFLTTVFFSIQILYLIAVTVYVL
                     GNVKLKDIAIESKRLRRWKTVNYLFYYLFATIYFIEAFFAEYRNLKQVKGGNLVLAQI
                     RDVLKNVSSLSPEINTSVTDLLSFEREKEIHNYRANYLGIFFLALLVIVHSL"
     gene            3116..3922
                     /gene="truA"
                     /locus_tag="PKM80_01890"
     CDS             3116..3922
                     /gene="truA"
                     /locus_tag="PKM80_01890"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNV92687.1"
                     /translation="MARYLIKICYDGTAYCGWQKQKNGLAIQEVMENALQIFTCEKNS
                     LIAAGRTDAGVHSLGQYAHFDYMGSMKEYQLLLAFNRYLPDDIKVLEIKKVSPALSAR
                     YQAYERSYRYILAKEKNPFNRNYTGFIPYLTLKLERMQAAATYLIGKYDFSSFGRNNP
                     EIPNRMCELKVCDINEQTDYFSFDFVADRFLHNMVRRMVGTLANISHFNLPPETISDI
                     LEKKCPRQNLVITAPASGLYLMNVKYPDKFLDESYLPELQENNGEISDLP"
     gene            4006..5271
                     /gene="hisS"
                     /locus_tag="PKM80_01895"
     CDS             4006..5271
                     /gene="hisS"
                     /locus_tag="PKM80_01895"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNV92688.1"
                     /translation="MKYNIPRGTFDILPEDSYKWQFLQDSFRKFAICFGYQEITTPIF
                     EVSELFERSSGESSDVVQKEMYRFCDKKGREFALRPEGTAPVVRSYVENNLDKLSKRS
                     KLFYLGPMFRYDRPQAGRYRQFYQYGIEFIGSNNPYYDAEVIAISMLWLNSLGLNKLR
                     LEINSVGCPDCAALYDKALQEYYRPFLGKLCPDCQNRFNTNPRRLLDCKVPSCIELRD
                     DAPSQLNYLDEPCEKHFEAVCNYLQAMKIKYIINPRIVRGLDYYTNTAFEIVYEGLGA
                     QNSLAGGGRYNGLIEQIGGKSVPAIGFAGGFERLLIALEEEKINYAEKPKPDAYLVCL
                     GENVRLEVLTYLNDLRIKGLIVDYDPDKNSMKAQFKAADASGAKYAIIIGEDELANKT
                     INLKNLQTGEQIRVAMNAEDTKQRIRAKI"
     gene            5522..6358
                     /locus_tag="PKM80_01900"
     CDS             5522..6358
                     /locus_tag="PKM80_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV92689.1"
                     /translation="MNAINFFKTKKLDLIVDFYTSVLALPVWLNQDSCIIFRAGNQLL
                     GFCRCDISEISGTITFFCKDRKEVDAIYSRLKAIAKTAPKPNLAYNIYNFWAADPEGR
                     SLEFQCFDHQLEPYITLEEGLKTRRSIRKYKSEPVPEELLNKVFELCRYSPTACNLQS
                     YYYIVIKQREILEKIVSLRGPSGKPILASPYAIAVASKGDISHRKIQDACIAAYHLML
                     AAKAYDLGTCWVTDMDKDEIKDLLGIPHQDYIACLTPIGYPAEHFPVPERHQVKDFVR
                     YL"
     gene            6355..7314
                     /locus_tag="PKM80_01905"
     CDS             6355..7314
                     /locus_tag="PKM80_01905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNV92690.1"
                     /translation="MKPLLFLTRLLPEPAIKLLEDNFTLKINPYNRSLTKQEIMENIK
                     DADALICLLTDIIDKEIIDAAPKLKVISNYAVGYNNIDIEYAIKCGIVVCNTPGVLTE
                     TTADLTWALIMATCRRITESERFIRSGKFKGWEPMLMLGQDVNNKTLGILGMGRIGQA
                     VAKRAVGFNMQIIYHNDIPLNQNLPYEATEVDFNTLLKEADILTLHIPLTENTYHLIG
                     KNELEIMKDSAVLINTSRGPIIDEQELIRALSTKKIFAAGLDVYEQEPYIPPELLALD
                     NVVLLPHIGSASIETRTKMALLAVENAISVIEGRKPPAMVKLT"
     gene            7524..8378
                     /locus_tag="PKM80_01910"
     CDS             7524..8378
                     /locus_tag="PKM80_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92691.1"
                     /translation="MKHIRFLLPVILFTLCGTVNAQLAQIIDFFVPQTVPVTPQESFV
                     RYMRGGYLTSGNYYQYLNPDEGFEHIESASVVILHYTYSKFSSQTVPKPLPMVTFGIL
                     DNVAAIQGSFNGDLKNSYFMDVLSLSGSYTYPLLNYNAYLNAGIKTAATYDAPYADKI
                     TALGVEHLFVESIECEYDPANYTYRVTKINQPEPYLHFSAEASARLGVRIYNNWFLAL
                     ALGVRQSPKVEGRWFMKDKVNSWLAGNAYYDTDWDMEQTIDKSLPKEVYFLGGTDFFL
                     CLSVSPFY"
     gene            8444..>9510
                     /locus_tag="PKM80_01915"
     CDS             8444..>9510
                     /locus_tag="PKM80_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV92692.1"
                     /translation="MQKNTKCSNMGFFSGLSEDVLQELTKDLERIQFKKGEAIITEHS
                     WGDNLFFICKGKVEINKGLHNPETPFAQLSILQAGDFFGEMGIIEDSPRSASVVALED
                     VELMIIPREAFHNMIFAYPLIMLNLVRTISGRLRSTNERFVELVDEMIKKNRLMAIGL
                     AASKIIHDIKTPLTVIVLTAQVLENVFPEGREFTENIIKQTKLIDQLVHEILDFAKGT
                     ETPPLIQQVDLDVFFRELQEMYFPNLKERKIDFIVENRVPNYVHFDEGKIRRVLINLI
                     KNAIEAMPVEGEIKILSSISSGWLQISVIDNGPGIPQKVQEDLFQPFVSDGKNYGTGL
                     GLAICKKLVQEHKGRLEYIPI"
BASE COUNT         3113 a         1571 c         1810 g         3016 t
ORIGIN      
        1 aattgaactt gttcgttcta agcgtttgga tgaaatttgc tctgaaatta attgacagga
       61 atatagcatt ccgcggttat tgaattatga aatgccgagt aataattatc tgtctgcttt
      121 gccttatcgg gctttttgcc tgtgaggtaa atactatgta taatgccaga aattatttta
      181 aatcggcaca atccaaaccg ctaaacagta atggtagacc aaatgctcaa gcaattgatg
      241 aatatacaaa agcgataaag aaatgcggaa aaattattgc tactgataaa aaaggcaaaa
      301 gggttgaaga agcatactat ttgatggcaa aatcccttta ctataaaggt aatagcgctt
      361 tccaggcaaa agaccaattc caaaatctgg taattcgctt tcccgatagt aaatatgttc
      421 ccgaagcata tatctacata gccaaaattt taagagaaac aaatcaaccc aaagaggctg
      481 aaaaactact cgatgaattt ttacgcaacc ctaagtaccg taaacatcat ccaatggctc
      541 tatccgtttt agctgatttt gctatacagg ataaagatta tattaaagct caacactatc
      601 tggaaaggat aattaacgaa tttcctaaaa caaaagaata tcgcgaggca tatttccttt
      661 ttggaaagaa ctactatgaa caaaaggact ttgccaaatc cctggaagca tttaaaaaaa
      721 tgcaaaacgc tcgtggaatc gataaagcaa aaaaattgga tggaacttat tatatcggat
      781 taaatgaatt ggaattggga gaagcggaaa aagcactcaa aactgcaaaa ggattgttaa
      841 aagcagaatc tcgtcctgat aaattaccct tcgtccgttt attgaaagca agagctcaat
      901 ttgctttggg ggatacaact gcagccagat ctgaaattga acttatcaac aaaaattatc
      961 ctcgcacagc aggttctgca ggtggatatt attacttggc agaacactat tattacaatc
     1021 aaggagatat tcataaagca attgctagtt ataatagagt acgaactgaa ttttcttcat
     1081 ctcctcttgc agcatctgct caggtaaaat cttcagcctt aggtttggtc tctccccgcc
     1141 aaggtcttga ttgtgaaaaa aatctaagca attttttgga ttatcaatat caggctgcag
     1201 aaagcttttt gaatgtatta gctttaccag attctgccat agcacgctat agaattgtta
     1261 ttgctcaaaa agatattctg gtaaacagat gtgattcttt actagctctt atcagtaaaa
     1321 ataatgcaat tctggattcg ttaagagcca ttgtaccgat acaggaaatt tctgaagaag
     1381 atagtatagg tatagtagat aaaactgaat tgcttaccca attagatgat cttcttgatg
     1441 ttagcacttt tacagatagt ttatcaagca ctgctgattc cttaaaaata acgaaaccgg
     1501 aaatagctgc tgaagatagt ttactaaagg ataacattat tgcaatacag gatagctctt
     1561 ctaaccagat agatacttta gttgtatcaa ttcgttctga aattaccaga ttggaacagg
     1621 aaaacgagtt atgtcaagac agaataaaca ctctggaaga gattatagag cggtttgata
     1681 gtgaaataat tcctttttgt ttgtttgcta taggctctat ttatcataac aactatccag
     1741 aaagtcctga aaatgcagaa attttaaccc aaatgcaaga acaatatcca gagaataaat
     1801 ttacaaaagc gctatatgcc ttacagaatg atctgccagt gcgtttaatt gatcctttag
     1861 aagaacgaga agaacagctt ttagagctgt atctggaaca aatatacact gctccagatt
     1921 cggctttggt aggtcttgaa gaacttaccc gttcgcgcta tccaaaaatt aagctggccg
     1981 ctaattatcg tttgggatgg ttttacagtt ttgaacaggt cgatacctta ttggctaaac
     2041 cttatttaaa tgctgtttta gaagaacctg aggctggcga atatgccact atcacacaaa
     2101 gattctttaa tggtgatatt ttcttgctaa gaggagctcc taaatttgat attcctgttg
     2161 ctgattctac tgtagtagat tccacttctt taaatattaa cgaaaacaag aaagaactac
     2221 ctcttccgga gggagcagag atacataaat ccttgtcgga tagtttaaaa atatataatg
     2281 aaattcctgt tttagttgat actttggcta agattattcc tccggtagaa aacaaacagg
     2341 aagaagatat tagttcactt gaaactgaaa ctccggaagt aaatactccg gaaccagaag
     2401 aaaatcctgt tcctactctt ccagaagagt cagttccaga aaaaaaagaa gaaatacccc
     2461 ttgaatgaag ttcatatctt atccaatttc gggacagcat ctctatttga agttagctgt
     2521 ttttttaaca ggtctggcaa taggagtaga ttcttcgctt cgccagctat tgattcagct
     2581 gattttgata ttggttattt taggttttga accttcttta tatcggttat ttttaaattc
     2641 tctaaaaaga ttattgcctt tttttgctgg ttattgggtt tttgcaactc tttttgctca
     2701 agatttttta acaactgtgt tcttcagtat acaaatacta tatttgattg cagttacagt
     2761 ttatgttctg ggaaatgtta aactaaagga tattgccata gagagcaaga ggttacgcag
     2821 gtggaaaacg gtaaactatt tgttttatta tctgtttgct accatttatt ttatcgaagc
     2881 attttttgct gaatatagaa atctaaaaca ggttaaaggt ggaaacttgg ttttagctca
     2941 gattagggat gttctaaaaa atgtatcttc cctttcgcct gaaataaaca catcagttac
     3001 tgatttatta agttttgagc gcgaaaaaga aatccataac taccgggcta actatctagg
     3061 aatattcttc ctggcgttat tagttatagt gcacagtttg taatacagag gaaaaatggc
     3121 aaggtactta ataaagatat gttatgatgg cacagcttat tgcggctggc aaaaacagaa
     3181 aaacggactt gctattcaag aagtaatgga aaacgcatta caaatcttta cctgtgaaaa
     3241 aaattcttta attgctgctg gaagaacaga tgccggagtt cattcattag gccaatatgc
     3301 gcattttgat tatatgggtt caatgaaaga atatcagttg cttttagctt tcaaccgata
     3361 tcttcctgat gatattaaag tgttggaaat aaagaaagta agccctgcat taagtgctcg
     3421 ctatcaagcg tatgaaagaa gttatcgtta tattttagct aaggaaaaaa atccctttaa
     3481 ccgtaattat actggtttta taccctattt aactttaaaa ctggaaagaa tgcaagcagc
     3541 agctacctat cttataggaa aatatgattt ttcttctttc ggacgcaaca atccagaaat
     3601 acccaataga atgtgtgaat tgaaagtatg cgatattaac gagcaaacag actattttag
     3661 ctttgatttt gtagcagacc gttttctgca taatatggtt cggagaatgg ttggaactct
     3721 ggctaatatt tcccatttta atcttcctcc tgaaacgatt tcggatattt tggaaaagaa
     3781 atgtccccgc cagaatttag tgataacagc ccctgcctca gggctttact taatgaatgt
     3841 gaaatatccg gataagtttc ttgacgaatc ttaccttccc gaattacagg aaaataatgg
     3901 tgaaatttcc gatttgccct aaaagggtaa ggactataat ataatatata tgctgaagag
     3961 aagataataa caaaatggaa tttaaaaatt taagaggaaa tactaatgaa atataacatc
     4021 cctcgtggaa cttttgatat tctgcctgaa gatagctata aatggcaatt cctgcaggat
     4081 agctttcgta aatttgccat ctgttttggc tatcaagaaa ttactacccc catttttgaa
     4141 gtttcagaac tctttgaacg cagttctggg gaaagctcag atgtagttca aaaagaaatg
     4201 tatcgtttct gcgataaaaa gggtcgcgaa tttgcattgc gtcctgaagg aacagcccca
     4261 gtggtgcgta gctatgtaga aaataatttg gataaattat ccaagcgctc aaaacttttt
     4321 tatttagggc ctatgtttcg ttacgaccgt ccccaagctg gtagatatcg tcaattttat
     4381 caatatggga tagaatttat cggtagtaac aatccctatt acgatgcaga agtaattgcc
     4441 atttctatgc tatggcttaa tagcttggga ttgaataaat tacggttgga aattaacagt
     4501 gtgggttgcc ctgattgtgc tgccctttac gataaagctt tgcaggaata ttatagacct
     4561 tttttaggta aactttgccc cgattgccaa aatcgtttta acactaatcc tcgccgatta
     4621 cttgattgta aagtgccttc ctgtatagaa cttagagacg atgctccttc tcaattaaat
     4681 tatctggatg agccctgtga aaaacatttt gaggctgtat gtaactatct gcaggcaatg
     4741 aagataaaat atataatcaa tcctcgaatt gtacgcggac tcgattacta tacaaatact
     4801 gcctttgaaa tagtttacga aggtttgggc gcacaaaatt ccttggccgg gggaggaaga
     4861 tataacggac ttattgaaca aatcggaggt aaaagtgttc ccgcaattgg ttttgctggt
     4921 ggttttgaac gcctgcttat agctttggaa gaagaaaaga ttaattatgc agaaaagccg
     4981 aaaccggatg cctaccttgt ttgtttagga gaaaatgtac gcttagaggt tttaacttac
     5041 ttaaatgatc taaggattaa gggacttatt gtagattatg atcccgataa aaattctatg
     5101 aaagcacaat ttaaagcggc tgatgccagc ggagcaaaat atgccattat tatcggagaa
     5161 gacgaacttg ctaataaaac tataaatcta aaaaatttac aaaccggaga acagataagg
     5221 gtggctatga atgcagaaga tactaaacaa agaataaggg ctaagattta agtttaaagt
     5281 tctaagtgca gggatttctt agttcaaaca ttgtagttgt cttggcacaa attactccgg
     5341 atttacataa tcctcttttc ttttttttct tattgtttaa aagaaatccg ctcaatccta
     5401 taatcctgta gtccgttatc taaaactaag tccattaaat cttgtgtcca tcctacttta
     5461 tatttgcttt tctgggtaaa atagattaca ttatactata gtaacttatt aaggatttat
     5521 gatgaatgct attaatttct tcaaaacaaa aaagttagat ttgatagtgg atttttacac
     5581 ttcagttttg gcactccctg tgtggcttaa tcaggattct tgcataattt ttagagctgg
     5641 aaatcaatta cttggttttt gtagatgtga tataagtgaa atctctggaa caataacttt
     5701 tttctgtaag gacagaaaag aagttgatgc tatctattcg aggctaaaag caatagccaa
     5761 aactgctccg aaacctaatc ttgcctataa tatctataat ttctgggctg ccgatcctga
     5821 aggtagatct ttagagtttc agtgttttga tcatcaattg gaaccatata taacattgga
     5881 agaaggtttg aaaaccagac gcagtatccg taaatataaa tctgaaccgg ttccagaaga
     5941 gcttttaaat aaagtttttg aactttgccg ctattctccc actgcttgta atttgcaaag
     6001 ttattattat atagtaatta agcagcgaga aattctggag aaaattgttt ctttaagggg
     6061 tccttccgga aaaccaattt tagcttctcc ttatgctatt gccgttgcct ctaaaggaga
     6121 tatttctcat cggaagattc aggatgcctg tattgctgct tatcatttaa tgctggctgc
     6181 taaagcttat gatttgggaa cctgttgggt aaccgatatg gataaagatg aaattaagga
     6241 tttgctggga attccgcatc aagattatat tgcttgctta actccaattg gctatcctgc
     6301 tgaacatttt cctgttcccg aaagacatca agtgaaggat tttgtgaggt atctatgaaa
     6361 ccgcttttat ttttaactcg tttgcttccg gaacctgcaa taaagcttct ggaagataat
     6421 tttactttaa agatcaatcc ctataaccgc tctttaacta aacaggaaat tatggaaaac
     6481 attaaagatg ccgacgcctt gatctgctta ctaaccgata ttatagataa agaaattata
     6541 gacgcagctc ctaaattgaa agttatttcc aattatgcag tcggctacaa taatatcgat
     6601 attgaatatg caataaaatg tggaatcgtg gtttgcaata ctcctggagt tttaacagaa
     6661 acaactgctg atcttacttg ggctctaatt atggcaacct gtagaagaat cacagaaagt
     6721 gaacgcttta tccgaagtgg caaatttaag ggttgggaac caatgctgat gttaggtcag
     6781 gatgttaata acaaaacctt gggaatttta ggtatgggta gaatcggtca ggcagttgct
     6841 aaacgcgctg tcggttttaa tatgcagatt atttatcata atgatattcc tcttaaccaa
     6901 aatttgccct atgaagcaac tgaagtagat tttaacactc tactaaaaga agcagatatc
     6961 ctcacactac atatccccct aacggaaaat acctatcatc taatcggaaa aaatgaattg
     7021 gaaattatga aagatagcgc agttctaata aatacttctc gcggtcctat tatagatgaa
     7081 caagaactga ttagggcact ttccacaaaa aaaatatttg ccgctggatt ggatgtttat
     7141 gaacaagaac cttatattcc tccggaactt ttagccttgg ataatgtagt tttactccct
     7201 catattggat cagccagtat tgaaaccaga actaaaatgg ctcttctggc tgtagaaaat
     7261 gctatttccg ttattgaagg aagaaaaccg cccgcaatgg taaagttaac ataatattag
     7321 acgcgaagat tacacatatc catctgcatt caataaccag ccggagtatt gaacattgta
     7381 ttagaacgca tagttgtgca ggcttttttt cagtgagact ttatagctta ctccatttca
     7441 caaatgaagt aaataaataa ttattcttga cagatattgg cattattgaa ataattaaat
     7501 caataaggaa agagagtttt atcgtgaaac atattcgttt tctgctgcct gttatattat
     7561 ttactctgtg cggaaccgtt aatgcacaat tggctcagat aatagatttt tttgttcctc
     7621 aaacagttcc ggttacacct caagagtcgt ttgttcggta tatgcgagga ggatatttaa
     7681 cttctggaaa ctactatcag tatctgaatc cggatgaggg ttttgaacat atagaatcag
     7741 cttctgtagt tatattacat tatacctact ccaaattttc ctctcaaact gttcccaaac
     7801 ccttacctat ggttactttt gggattttag ataatgttgc tgcaattcaa ggttctttta
     7861 atggtgacct aaaaaactca tattttatgg atgtcttatc gttatccggg agttacacct
     7921 atcccttgct gaattataat gcatatctaa atgcaggaat aaagacagca gctacttatg
     7981 atgctcccta tgctgataaa attactgcct taggggtgga acacttattt gtggaatcaa
     8041 ttgagtgtga atatgatcca gccaattata catatcgggt aacgaaaatt aatcagccag
     8101 agccctatct acatttttcc gctgaagcat cagctcgttt aggagttaga atatataata
     8161 attggttttt agctttggct ttgggagtta ggcaatcgcc caaagttgaa ggtagatggt
     8221 ttatgaagga caaagttaat tcttggcttg caggtaatgc ttattacgat acagattggg
     8281 atatggaaca gaccatagat aaatccctgc cgaaagaagt ttattttctg ggtggaactg
     8341 atttctttct ttgtctaagc gttagtcctt tttattaaga tgtaaaacaa ttgtggttaa
     8401 aattaggaag taatttaaca actatagcag atgtgaggta aaaatgcaaa aaaacacgaa
     8461 gtgtagtaat atgggttttt ttagcggtct ttctgaagat gtattgcaag agcttactaa
     8521 agatttagaa agaatacaat ttaaaaaagg tgaagcaatt attaccgaac actcttgggg
     8581 cgataacctt tttttcatct gtaaagggaa ggtagaaata aataaaggtt tacataatcc
     8641 cgaaacccct tttgcacagc tttctatttt acaagctggc gatttttttg gcgaaatggg
     8701 cattattgaa gatagccccc gctctgcaag tgtagtagct ttagaagatg tagaattgat
     8761 gatcatccca cgagaagcat ttcataatat gatctttgct tatccattaa ttatgttgaa
     8821 tctggtgagg acaatttcag ggaggttgcg cagtacgaat gaacgttttg tagaactcgt
     8881 ggatgaaatg atcaagaaga atcgcttaat ggctattggt ttagccgcaa gtaaaatcat
     8941 tcatgacatc aaaactccct taacggttat agttttaact gcgcaggttt tggagaatgt
     9001 ttttccagag ggtagagaat ttacggagaa tattattaaa caaaccaagc tgattgatca
     9061 attagttcac gaaatattgg attttgctaa aggaacagaa actcctccac tgattcaaca
     9121 agtagattta gatgtctttt tccgggaact tcaggaaatg tattttccca atcttaaaga
     9181 gcgaaaaata gattttattg tagaaaacag agttccgaat tatgttcatt ttgatgaagg
     9241 aaaaatccgt agagtgctaa ttaatcttat taaaaatgct attgaagcaa tgcctgttga
     9301 aggggaaatt aagattttga gtagtatttc ttctgggtgg ttacaaatat cggttataga
     9361 taatggtcca ggtataccac aaaaagtgca ggaagacctt tttcagccat ttgtatccga
     9421 tgggaaaaat tatggtaccg gtttaggatt ggcaatttgc aagaagttgg ttcaggagca
     9481 taagggtcgt ttagaatata ttcctataga
//
LOCUS       DAORHB010000034         9506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_6589_length_9506_cov_5.752407, whole genome shotgun sequence.
ACCESSION   DAORHB010000034 DAORHB010000000
VERSION     DAORHB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6589_length_9506_cov_5.752407"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKM80_01920"
     CDS             complement(<1..714)
                     /locus_tag="PKM80_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92693.1"
                     /translation="MNRKVIILFLAILCILAFTLQGQEIPSSSTFHNGNLKEDNFGLI
                     ALRQNSYSLIKLNQYATNLQDYQAEMKYKEYSSMPEWSKCVTLHSAHEYLCYDNTDYD
                     VSWQQFNSFNTLGVKSKFAGVEVCYNIMNFELDDDHSAQSYNLNKYGIGFGFPNSESL
                     RLFGWLMQSDYRDLIPNLSFTSYKAELAKRVISDSQITELGVEYEYYEVPDIYNAQFS
                     LYDSEYFPQYYRFSNPNTKL"
     gene            complement(910..1476)
                     /locus_tag="PKM80_01925"
     CDS             complement(910..1476)
                     /locus_tag="PKM80_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNV92694.1"
                     /translation="MKKLMLLTLCLLLFCSVLLAQADTPKDKAAPKAKTEELKKANPE
                     RPMQKCLEDLKLTDAQKKKWEELKTSFEKTKNTLQAEIKNLKIDLRTALKNENFQNAK
                     ELNKQIAAKRNALDDAKVDFLAARMKELTSEQKATLKKHLEQCPNCHNAPMQNKKAMQ
                     MRHSEKENCNNCRECGSADMPTKINIRK"
     gene            complement(1473..1925)
                     /locus_tag="PKM80_01930"
     CDS             complement(1473..1925)
                     /locus_tag="PKM80_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92695.1"
                     /translation="MSKRWLIAILLISVSFNLAFIGSIIYLHYIHPFPKPPMENEHRQ
                     ASRPPFGPPPFERDEEIRNLRKNFEDIKHSLMLELANDPVDMKKVNALIDSSLIAQNI
                     LEQKLAERMVAYRKTLSAEEAKEHFLRRAEFVKNHSNRQRKSQNRRTQ"
     gene            complement(1918..2364)
                     /locus_tag="PKM80_01935"
     CDS             complement(1918..2364)
                     /locus_tag="PKM80_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNV92696.1"
                     /translation="MRCSKAKRYLEKYLDGELDPHLQAKLNAHLDVCAQCKSWLVDAK
                     KLQLMLTALPMADFPSWVHNQIMDKVHRLDSQNPGFFRRYKLAPVTAMLTIALSLLIG
                     IKVGTVSYKNIVPEKQDYTISTSVAYGGFGENTILDYVSENGEQDE"
     gene            complement(2410..2898)
                     /locus_tag="PKM80_01940"
     CDS             complement(2410..2898)
                     /locus_tag="PKM80_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV92697.1"
                     /translation="MKKEHFEAFLTANEKRIYHYVLSLCSNEQDTLDIVQTVFIAVYQ
                     NLERIEEETALAYTYKIAHNKCLSFLKQKSRYVNIEPDKFNSMQDKNLNYKEPDYTVL
                     KNAIASLPPRLAAVIQLQYYEKMSYKEISTTLGISIKAVESLLVRAKRILRKKILQDK
                     EK"
     gene            3013..4269
                     /locus_tag="PKM80_01945"
     CDS             3013..4269
                     /locus_tag="PKM80_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNV92698.1"
                     /translation="MINKSVVYLSRKYLNGRKSKGISKTHYLCLIGITLGVLALLCVT
                     TVMNGFRFDIRNRITGTFSEIRIVANEGETVKNYSSICKQLQDMKFYASPVIRNELLL
                     KYEQVVLPSLCFGIEAEKHKSVSSLLHKPKNDNREIIQGIVAGNIAPEDFNSSGIALG
                     AGLASQLGVYLGDEIQVLSPMFNRPTAFGMIPRIRYLKVQAIFAAGMPEYDQNYSLIG
                     IDNAAFFSSYQDDEADYIEVKPSPNAPQNHIMQTLKKAFPQYQIEDWSSFDPNLYSAI
                     RFEKFLMFIIMLFMFIIASFNLTGNLLKTISQKKRELGLLKALGLTDKDLQTLFLLQA
                     LFLCTVGIIAGLGFGSILLLIQKYTGIVKLGLGGGEAIILPVKFLFTDYLLIVVVAYL
                     ITCLSVLLPLKRLNKINAVELIKRTV"
     gene            complement(4584..5957)
                     /gene="mnmE"
                     /locus_tag="PKM80_01950"
     CDS             complement(4584..5957)
                     /gene="mnmE"
                     /locus_tag="PKM80_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNV92699.1"
                     /translation="MSNVGEIICAPITPNGFSAISVIRLSGKGCIELVAKHFSQSRKL
                     LALSSHRLIFGTFYSESGEAIDEVLISVFREPNSYTGEDCLEISCHGNPNLVYNIIQT
                     LLLSCRMAKPGEFTLRAFLNGKMDLSQAEAVNDLIQAQASKAEKAALMQLKGKLSNYL
                     QELLNRITELRIRFELAIDFADQDLPMPDINLMKQELMDIIKTAEELKSTGEQGIKIR
                     EGIKICLTGAPNVGKSSLFNALLQHNRAIVTPHPGTTRDYLEECLSLNGFPIVIYDTA
                     GLREAKDDIEKEGIAKSYELMQRADLILYLVEAKQIANLELELPELIKLSTIPPELQS
                     KTLIVFSKADLMEENSPKISSGIYCSVKKENGLQALTKAILNRLMLSDELLTKPIIIN
                     NRHLAALSKCINSLHQADLSLKENLGFEFIAFELISASNALEEILGIITTDDLLDKIF
                     SEFCIGK"
     gene            complement(5950..7818)
                     /gene="dxs"
                     /locus_tag="PKM80_01955"
     CDS             complement(5950..7818)
                     /gene="dxs"
                     /locus_tag="PKM80_01955"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNV92700.1"
                     /translation="MILENITDPNQIKSLTSSELKILAKEVRNRIVEVVSKTGGHLAP
                     SLGTVDLTIALLYLFDPLVDRIVWDVGHQTYGWKILTGRNAQFDSLRQYKGLCGFTNR
                     DESPYDAFTTGHSSTSISATLGIACGRDLNNEKGHCIAVIGDGALTGGISFEALNHGG
                     HLQKDKFIVILNDNEMSISKNVGGLQKYMTRMLASKSYNVLKKQIWDLSLTLPSNIRR
                     SFIYGAQKLEESMMNILVPNIIFEDLGYKYVGPIDGHDIDQLLRIIKRVKNYMVGPVL
                     IHIVTQKGKGYAPAEKDAALFHGTGPFELKTGKQLSSGKQSWSDFMGSTLVNLAKKNP
                     KIVAITAAMIAGTGLTKFEETFPDRFFDVGIAEQHSVTLAAGMATKGIKPFVAIYSTF
                     MQRALDQVIHDVALPKLPVVFCIDRAGIVGEDGATHQGAFDLSFLNFIPNLIILTPST
                     AEELSAMLSWAADYQEGPVAIRYPRGAAIHSLEHQEPVQFDPFSVKIHSHEGKIALVA
                     CGDAIFIAEEVHKLLEKNKIQSKLVQLLSVKPLNEKALSDLASTCNYIFTFESNAVIG
                     GMGARITQLLSTYPVKVINFGYPDSFIPHGKISELLDEIGFTPAKLYNKISQILNE"
     gene            complement(7835..>9506)
                     /locus_tag="PKM80_01960"
     CDS             complement(7835..>9506)
                     /locus_tag="PKM80_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92701.1"
                     /translation="TKLKPSHKHPPTAIAILIDTSYSMDYAERGKSSLQYAKDILRKI
                     NTKANSDDRLIPVSSDENWNLMHSQIYAGTIPETLIDQLKITYNPLSLEEMIAYAETK
                     LSESQMPNREIYLVSDLRIPPSTIKTKIPVALIPLLETSSYENLACISANALPQLVDK
                     RNQQLIQFTIANYGNIEYKDVLVKAVVNDIKLAEKFVSVPPKSTITETITTELRSDGW
                     QSGYIEINDECQMMDNRCYFAFPFYSKPRIAVISKNDNLPAILATVLRVYSSATYQVI
                     SPDALSLSDLDQYQLFVVYECGAITPRLNEIFTNLRNHKTGLLFCIGNNLAADLKSYL
                     NNNFGLEIKDKTHKAVSIDNLNAHHYISSLISGKPLKNPLIVDYWQAVNKSAGVLVSA
                     QSFPMAVINGKQSLWLWNIASTENPFFIDPAFPVLAFRTLDYIAGSDFPESEAKIGQI
                     LTFDKLKLPTGETISSRRYLANEPGVYIFEPDTPRNYAMAINIDYSDSEDRTNFPKGI
                     KNLGDKWEEKLFFSRLGFDLWKILLAIAFALMILEIIIIKTEEARAKP"
BASE COUNT         2794 a         1748 c         1761 g         3203 t
ORIGIN      
        1 taactttgta ttaggattgc taaatcgata atattgggga aaatactccg aatcatataa
       61 gctaaattga gcgttatata tatcaggaac ttcgtaatat tcatattcta caccaagttc
      121 cgtaatctga gaatcactga taacgcgttt tgctaattct gctttatagc ttgtaaaact
      181 cagatttgga ataagatctc tataatctga ttgcatcaac cagccaaata gccgtaatga
      241 ttctgaattg ggaaaaccaa aacctatccc atatttattt aaattataac tttgtgcgct
      301 gtgatcatcg tcaagttcaa agttcattat attgtagcaa acctccacac cggcaaattt
      361 agatttcact cccagagtat tgaaagaatt aaattgttgc caagatacat cgtaatctgt
      421 gttatcatag catagatact catgagcaga atgaagtgta acacatttac tccactccgg
      481 catactggaa tactctttat atttcatctc tgcttgataa tcttgaagat ttgtagcata
      541 ctgatttagt ttaatgagag aatagctatt ctggcgtaat gcaattaagc caaaattatc
      601 ttcttttaga ttgccattgt ggaaagtaga gctcgatgga atctcttgac cctgtagggt
      661 aaaagccaaa atacaaagaa tagctaaaaa cagaattatc acttttctgt tcattttttc
      721 ctcctgaaaa caattttggt tataaatatt ataatggctg gctttgtcaa tgattttttc
      781 tgcatcattt gcatcattct tgcacagatt gattttagta aagaataaat acaaaaacca
      841 cgggaggagt agcccgtggt tttttctgtg tgttgctgag gagggattat tttggacttt
      901 ctatagtatc tattttcgga tatttatttt tgtgggcata tctgcggagc cacattctct
      961 gcaattatta caattttcct tttcagaatg tctcatctgc attgcttttt tattctgcat
     1021 tggagcatta tggcaattgg gacattgttc taagtgtttt ttcaaagtgg ctttctgctc
     1081 agaggtaagt tctttcattc tggcagccag aaaatctact ttggcatcat ctaaggcatt
     1141 gcgttttgca gcaatttgtt tgttcaattc cttagcgttt tggaaatttt catttttcag
     1201 agcagttctc aagtcgattt tcagattttt gatttccgct tgcagagtat ttttagtttt
     1261 ttcaaaactc gttttcagtt cttcccattt ctttttttga gcatcagtta gttttagatc
     1321 ttcaaggcat ttctgcatcg gtctttcggg attagctttt ttcaattctt ctgtttttgc
     1381 tttgggagca gctttgtctt taggggtatc tgcctgtgct aataacacac tgcaaaaaag
     1441 cagtaggcag agagttagca acattaattt tttcattgtg ttctcctgtt ttgagatttt
     1501 ctttgtctgt tggagtgatt tttaacaaat tcagcacgcc gaagaaagtg ttctttagct
     1561 tcttctgcac tgagtgtttt acgataggca accattcttt ctgcaagttt ctgttcaagg
     1621 atattttgag caattaagga cgaatcgatt agggcattca cttttttcat atcaacagga
     1681 tcattagcca gttccagcat taatgaatgt ttgatgtctt cgaagttttt tcgtaaattc
     1741 cttatttctt catcacgctc aaatggagga ggaccaaaag gtggacgaga tgcttgcctg
     1801 tgttcatttt ccattggagg tttaggaaat ggatgaatat aatgcagata gataatactg
     1861 ccaataaaag caagattgaa gctgaccgat atcagtagaa ttgcaattaa ccaacgctta
     1921 ctcatcctgc tctccatttt ctgaaacata atctaatatg gtgttttctc caaaaccgcc
     1981 ataagctaca gatgtggata ttgtatagtc ctgtttttct ggaactatat ttttatagct
     2041 gacagtgccc acttttattc caatcaacaa acttagagca attgttagca tagcggtaac
     2101 gggagcaagt ttatagcgac ggaaaaatcc tgggttttgt gaatccagac ggtgcacctt
     2161 atccataatt tggttatgaa cccaggaagg gaaatctgcc attggcaatg cagttaacat
     2221 tagctgcagt tttttagcat ccacaagcca gcttttacat tgtgcacata catcaagatg
     2281 ggcattaagc ttcgcttgta aatgcggatc aagttcgcca tcgagatatt tttccagata
     2341 gcgtttggct ttactgcatc tcatttaact gctccttatt aaaatttttg gttccaatta
     2401 ttataccttt cacttttctt tatcctgcaa tatttttttt cgtaaaatcc gctttgctct
     2461 caccaataaa gattcaactg cctttatgct gattcccaaa gttgtagata tctccttata
     2521 ggacatcttt tcataatatt gtaactgaat tacggcagct aaccgaggtg gtagagaagc
     2581 aatagcattt ttcagaactg tgtaatcagg ttccttatag tttaagtttt tatcctgcat
     2641 agaattaaat ttatctggct cgatatttac atatcgggat ttttgcttta gaaaactgag
     2701 gcatttatta tgcgcgattt tatatgtata tgcaagagct gtttcttcct ctatgcgttc
     2761 caaattttga taaacagcaa taaacacagt ttggacaata tctagagtgt cctgttcatt
     2821 ggagcatagg gatagcacat aatgatatat acgcttttca ttggcggtta aaaatgcctc
     2881 aaagtgttct tttttcatag ctgtttattc tgaagtgtcc ttcaaatttt ctttttgtcg
     2941 tgcaaaaaat aaaccaaacc taaaacaaag cttgacttat tgctatgtta tcagttattt
     3001 ggtcaactta gtatgataaa taaaagtgtt gtttacctgt caagaaaata tctaaatggg
     3061 cgtaaaagca aaggcatcag caaaacgcat tatctgtgtc ttatcggcat cactttaggc
     3121 gttttagctt tgctttgtgt tactactgtg atgaatggtt ttcgctttga tattcgcaat
     3181 cgtattacag gtactttttc cgaaattcgc atagtggcta atgagggaga aaccgtaaaa
     3241 aactacagtt caatatgtaa gcaattgcag gatatgaaat tctatgccag ccccgtgatt
     3301 cgtaatgagt tattattgaa atatgagcaa gttgttttgc catccctctg ttttggcatc
     3361 gaggcagaaa agcataaaag tgtttcttct ttgcttcata agccgaaaaa tgacaaccgc
     3421 gaaattatcc aaggcatagt agccggaaat attgctccag aggattttaa ctcttctgga
     3481 atagctttag gagctggctt ggcttctcaa ttaggtgttt atttggggga tgaaattcag
     3541 gttctttctc caatgttcaa tagacctaca gcttttggaa tgatacctcg tatccgctat
     3601 ctaaaagtgc aggctatttt tgccgctgga atgcctgaat atgaccaaaa ttatagtttg
     3661 attggaattg ataatgctgc ctttttttca tcttaccaag atgatgaagc ggattatatt
     3721 gaagtaaaac cctctcctaa cgctccacaa aatcatatta tgcaaaccct taaaaaagca
     3781 tttccccaat accagataga agattggagc agttttgatc ccaatctata ttctgctatc
     3841 cgcttcgaaa aattcctgat gtttataatt atgttgttta tgtttatcat tgccagcttc
     3901 aatcttacgg gaaatttgct gaaaacgatt tcccaaaaaa aacgggaatt gggtctacta
     3961 aaagcattgg gactaacaga taaagacctg caaactcttt ttttgctgca agcacttttt
     4021 ttatgtaccg taggaataat cgcaggactt ggtttcggct cgattctatt gctcattcaa
     4081 aaatatacgg ggattgtgaa attgggattg ggtggtggag aagcaataat tttacctgtg
     4141 aaatttcttt tcacggatta tttactaatc gtagttgttg cctacctaat cacctgttta
     4201 agcgtgctac tcccactaaa aagattaaat aaaatcaacg ctgtagaact catcaaacgc
     4261 actgtttagt taattaactg ctgcgcatat actcccgaaa agattccctt atcattccca
     4321 tatcgccata agggatgtat atgggaataa tatgatgaac aacagagaaa tgcttaatgc
     4381 tattattgga tcattttttc gtggggatag attatttgtg agtgttcaat cacttgggac
     4441 atagaggtaa gcttttaggg ctatcagcta tccaattttt tgctttcttt tttccgattc
     4501 ttgatctatt cttttataat gaggttataa atacattttg tacctagata gataatgata
     4561 gtaggagaga aaaaaacctg tcgttatttt cctatacaga actcgctgaa gatcttatct
     4621 aatagatcgt cggtagtgat aattcctaaa atttcttcca gggcattgct ggcagaaatt
     4681 agttcaaaag ctatgaattc gaagcccaga ttttctttta gtgaaagatc tgcctgatgc
     4741 agagaattta tacatttaga aagagccgcc agatgtctgt tattaattat gattggtttt
     4801 gttagcaatt cgtcgctgag catcaatctg tttaaaattg cttttgtaag tgcttgcaaa
     4861 ccattttctt tttttacact gcaataaatt ccgctggata ttttaggtga attttcttcc
     4921 atcaaatctg ctttactgaa cacgataagg gttttggatt gtagttcagg aggtattgta
     4981 gatagcttaa tcaattctgg aagctcaagt tcaagatttg cgatttgctt tgcttccact
     5041 aaatagagaa tcaggtctgc tctctgcatc aattcgtagc ttttggcaat tccttctttt
     5101 tctatatcat cttttgcttc gcgcaatcct gctgtatcat aaataactat aggaaatcca
     5161 tttagggaaa gacattcctc caaataatct cttgttgttc ctggatgggg ggtaacgata
     5221 gctctgttat gttgcagcaa agcattaaaa agtgaggatt taccaacatt gggtgcacca
     5281 gttaaacaga tttttatccc ttctcgaatt ttaattccct gttcacctgt acttttcagt
     5341 tcttctgctg ttttaataat atccatcagc tcttgtttca ttaaatttat atccggcata
     5401 ggcaaatcct gatcagcaaa atcgatagcc aattcaaagc ggatacgcag ttctgtgatt
     5461 cggtttagca attcctgtaa ataattgctc agtttgcctt taagttgcat aagagcagct
     5521 ttttcagctt tagatgcttg ggcttgaata agatcattaa ctgcttctgc ttgactcaaa
     5581 tccatttttc catttaaaaa agcgcgcaga gtaaattccc ctggttttgc cattctacaa
     5641 gagagtagta aggtttgtat aatattatat acaagattag gatttccatg gcaggatatt
     5701 tcaagacagt cctctcccgt ataactattt ggttctctaa aaaccgagat cagaacttca
     5761 tctattgctt cgcctgattc ggaataaaaa gtaccaaaaa tgagccgatg ggaagataga
     5821 gcaagcagtt ttcggctttg ggaaaagtgt ttggcaacca gttcaataca gccctttccg
     5881 cttaagcgaa tcaccgaaat ggctgaaaat ccgttgggag tgataggtgc acaaataatt
     5941 tcgccaacat tactcattta ggatctgcga tattttattg tagagtttag caggtgtgaa
     6001 accaatttca tccaatagtt cagaaatttt cccatgcgga ataaagctat ccggatagcc
     6061 gaaattgatt accttcactg gataagtaga aagtaattga gttattctgg ctcccattcc
     6121 tccgataaca gcattgcttt cgaaagtaaa gatataatta caagtagaag ccaggtctga
     6181 aagtgctttc tcgtttagcg gtttaacgga tagcaattga acaagtttag actggatctt
     6241 attcttttcc agcagcttat gaacttcttc ggctatgaag atggcatctc cacaggcaac
     6301 taaggcaatt ttcccttcat gagaatggat ttttactgaa aaaggatcga attgtaccgg
     6361 ttcctgatgt tccaacgaat gtattgctgc accccgagga tatctaatag ccacaggacc
     6421 ttcttgataa tctgcagccc aggaaagcat cgcggaaagt tcttctgctg tagaaggagt
     6481 aagaataatt aaattgggga taaaatttaa gaaagagagg tcaaaagctc cttgatgcgt
     6541 agctccatct tcacctacaa tacctgctcg atcaatacaa aaaacaacag gtaattttgg
     6601 aagagccaca tcgtgaataa cctgatctaa agctctttgc ataaaggtgg aatagatggc
     6661 tacaaaaggt tttatccctt ttgtggccat tcctgctgcc aaggttacag aatgttgttc
     6721 agcaatgcct acatcgaaaa agcgatccgg gaaggtttct tcaaatttgg ttaatccagt
     6781 tccggcaatc atagcagctg taattgctac aattttagga tttttctttg ccaaatttac
     6841 cagagttgag cccataaaat cgctccaaga ttgttttccg ctgcttaatt gctttcctgt
     6901 ttttagttca aagggtccgg ttccatgaaa gagagctgca tctttttctg cgggagcata
     6961 tccctttcct ttttgggtta caatatggat aagaacgggg ccaaccatat agttcttaac
     7021 tctttttatg atgcgtagaa gttgatctat atcatgacca tctatggggc ctacatattt
     7081 gtagccaaga tcttcaaaaa tgatattagg caccagaata ttcatcatag attcttccaa
     7141 tttctgtgca ccgtaaataa aactgcggcg gatattggaa ggcaaagtta agctcaaatc
     7201 ccaaatttgt tttttcagta cattatagga tttactagca agcattcgcg tcatatattt
     7261 ttgcaaacca cctacattct tagagatgga catttcgtta tcgtttaaaa taacaatgaa
     7321 cttatccttt tgcaggtgtc cgccatgatt taaagcctcg aaactaatac caccagttaa
     7381 ggcaccatct ccaataacag ctatacaatg ccctttttca ttatttaaat ctctgccaca
     7441 ggcaattccc aaagtggcag aaatggaagt actgctatga cctgttgtaa aagcatcata
     7501 gggactttca tcccgattgg taaaaccgca tagtccttta tattgcctta agctatcaaa
     7561 ttgagcattg cgtccagtta agattttcca gccgtaggtt tgatggccca catcccaaac
     7621 aattctatcc acaagcggat caaaaagata tagcaaggca atggtaagat cgacggttcc
     7681 taaactggga gctaaatgac cacctgtttt agaaacaact tcaacgatac gattgcgaac
     7741 ttccttagcc aggattttta gttcgctgga ggttaagctt tttatttggt ttgggtctgt
     7801 aatattctcc agaatcatat ctactccttt tggcttacgg ttttgccctt gcctcctctg
     7861 tttttataat aataatttcc aaaatcatca aagcgaaagc aatggcaagt aaaattttcc
     7921 aaaggtcaaa acctaagcgt gagaagaaaa gtttttcttc ccatttatcc cccaagttct
     7981 ttattccctt gggaaaatta gttctgtcct cggaatcgct ataatctata tttatggcca
     8041 ttgcatagtt gcgaggagtg tcaggttcaa aaatatatac tccaggttcg ttagctaaat
     8101 accgtcggct gctgatagtt tcacctgtag gtaattttaa tttatcaaag gtaagtatct
     8161 gaccaatctt cgcttcactt tctggaaaat ccgaacccgc tatataatct aaggttcgaa
     8221 aagccagaac tggaaaagcc ggatcgataa aaaatggatt ttccgtagag gcaatattcc
     8281 atagccaaag tgactgcttt ccgttgataa cagccattgg aaaggattga gcagaaacta
     8341 aaactcccgc acttttatta actgcctgcc aataatccac tattaaagga ttttttagcg
     8401 gttttccaga aataagcgaa ctaatataat gatgtgcatt gaggttatct atagatacag
     8461 ctttatgcgt tttatcctta atttccagcc cgaaattatt attcagatat gatttcagat
     8521 ccgcagcaag attattacct atacaaaata ataatccggt tttatgattt cgcaagtttg
     8581 taaaaatttc attcaagcgg ggagtaatag ctccacattc ataaaccaca aagagttgat
     8641 actgatccag atcactaagt gaaagtgcat caggtgaaat tacttgatag gttgcagagc
     8701 tgtaaactct taaaacggtt gcaagaatag ctggcagatt gtcattttta gagattacag
     8761 ctatgcgggg tttactataa aaaggaaaag caaaataaca gcggttatcc atcatttgac
     8821 attcatcatt aatttcaata tatccacttt gccagccatc ggaacgcaat tcagttgtta
     8881 ttgtttcagt tattgtactt ttgggaggaa cggaaacaaa cttttcagcc agcttgatat
     8941 cgtttaccac agctttaacc agaacatctt tatattcaat gttgccataa ttggctatgg
     9001 taaattgaat taattgttga ttgcgcttat caacaagttg aggtaaagca tttgcagaaa
     9061 tgcaagccaa gttttcatag ctacttgttt ccagcaaagg aataagagca actggaattt
     9121 tggtttttat tgttgatggg ggaattcgga gatcgctaac taaataaatt tcccgattgg
     9181 gcatctgact ttcggaaagc ttagtttctg cgtaagcaat catttcttcc aaagaaagag
     9241 gattataagt gattttcagc tggtcaatta atgtttcagg tatagttccc gcataaattt
     9301 gactgtgcat caagttccaa ttttcatcgg aacttacagg aataagacga tcgtctgaat
     9361 tagcttttgt attaattttc ctaagaatat ctttagcata ttgcagagaa ctttttcccc
     9421 tttctgcata gtccatactg taagaagtat ctatcaaaat agcaattgcc gttggtggat
     9481 gtttatgcga aggttttaat ttggtt
//
LOCUS       DAORHB010000035         9213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_6928_length_9213_cov_4.659969, whole genome shotgun sequence.
ACCESSION   DAORHB010000035 DAORHB010000000
VERSION     DAORHB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6928_length_9213_cov_4.659969"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1958)
                     /locus_tag="PKM80_01965"
     CDS             complement(90..1958)
                     /locus_tag="PKM80_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92702.1"
                     /translation="MKKRILFFFLSLFLLCSPYWKAQTQGLDWTVYSSQQVLSIENAL
                     QYRFYPTAKTSLVINSKSNIENRLNFNQEAKNADLNVGLELMSKHFMHTISSDYQTFY
                     DNSDLEPAAYVNKTAILGYHINYSPVDSLNIGIFSKGILRNEQDRYVNGYSLSSEGYW
                     FGSNASYSNYILNSIAGISANAERKRMDWESFDYANLGANYNYYGNYLNFACLANYNY
                     HSEDIYVLAAPEQNEIRSNYQLSDNQLRWNFDISGNLQYSPEDRVQLEIRDNYSLRQT
                     SYSKSEIRNNTDHYNQAQLHLDYQISQKVSWQSDLAHTYAIKDYYYDLNTRQTDNRHI
                     GSRINWEYSEGDSLIAAYFVNLQIIKFPEDDNQWDNDLLTHNLRFGWKHYWHERVVLG
                     NWFGYSFSKDVYIDSLLSANNKEISSWTMFPECKFLIGDRIAFFQTYQIRADYSDYIY
                     NTGKNNTLYRQLGYQYNLVFDTFPLIARTLDPRWLELPFRNSPENAFMIDLGFAYEEN
                     QYAQQKQSYYELQTKNRRWTASIDFRHDIKNLYWTLSPQYTWGTWKELTFNLNSACLF
                     NNNSLIEFKLAPFIDNFENIDSVSFHNFSANKFFKMVKQCSEAADWRLSTTLRLYF"
     gene            complement(1909..2691)
                     /gene="rsmA"
                     /locus_tag="PKM80_01970"
     CDS             complement(1909..2691)
                     /gene="rsmA"
                     /locus_tag="PKM80_01970"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNV92703.1"
                     /translation="MKALKALGQNFLIDKTIASEIVALGDLHKGDKVWEIGPGTGILT
                     DEILSWNVQLRAFELDKRLQKYLSEHYQGRFVLESTDILQANWDSLIQQDGYPLKLIA
                     NIPYQITSPLLALLEKHSKFFSRIVLMVQKEVAQRLCAQSHSKHYSPLTIRLHLVYDI
                     YSKIQVNKEKFNPVPKVDSTVILLLPRTDKPIIKYPESFNNIIDAAFAHRRKTLANNF
                     IPVLGKEKTKELENLSQINFRQRGEELEEKDFILLSELVSSL"
     gene            complement(2755..2931)
                     /locus_tag="PKM80_01975"
     CDS             complement(2755..2931)
                     /locus_tag="PKM80_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92704.1"
                     /translation="MAKNNRGKGIKKLPNSGRGECPVCHRTGIKLLNEVKVGEKTYKV
                     CKTCKSVAAKKLSA"
     gene            complement(3251..4309)
                     /locus_tag="PKM80_01980"
     CDS             complement(3251..4309)
                     /locus_tag="PKM80_01980"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNV92705.1"
                     /translation="MNSENKKYLFDLLQTHSPSGFETPAQVLTNAYLSNYCEDITIDI
                     NGNLIAHKKGSGKHKIMIIGHADEIGFMVNYIDESGFIYVKALGGFDVNMLPGLRVDI
                     HHNDNIVHGIIGKKPIHLLRNSDEPSKLKLEDLWIDIGARNKEDAQNLVSIGDTITFS
                     SQIEELNEELIVSKATDNKVGVYIASAVMKELWEKDIKADYYAVSSVGEETTMRGAIT
                     SAYRIDPDIAVAVDVTFTSDIPGVDKHIYGDVSLGKGPVLCLGSALHPAINKKLTELA
                     KEHNIPLQIEISPSRTGTDADAVFASRQGTATAILSIPNRYMHTPNEIISLADLDNAV
                     QLLVEFVLSIDDTFELKR"
     gene            complement(4319..4714)
                     /locus_tag="PKM80_01985"
     CDS             complement(4319..4714)
                     /locus_tag="PKM80_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV92706.1"
                     /translation="MKVIHYDEAELEPVDAEGAKGAQIRWLIAQKDGAPTFAMRMFEI
                     EPGGHTPFHQHEWEHEVYCLSGNGAIVTERGEMAFSTDDAIFVEPNLTHTFKNVGKET
                     LKFLCLIPHEKPVIKKALNPFADEEANNC"
     gene            complement(4782..5888)
                     /gene="ribD"
                     /locus_tag="PKM80_01990"
     CDS             complement(4782..5888)
                     /gene="ribD"
                     /locus_tag="PKM80_01990"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNV92707.1"
                     /translation="MTDFEINKYFNLAIQAAEKARGKCSPNPFVGSVIVKNNKVIAIG
                     WTLQYGGDHSEIQAIKHAGKEAEGATIFVTLEPCSHYGKTPPCAKAIIEAGIQEVYIG
                     IPDPNPIVSGNGIAMLKNAGIKVHCGFYEDKIKKQLEYYLCHIEKGRPFVTLKTALSL
                     DGKYAAGDGSSKWITGEKARKYVHKLRSENDVILTGIKTILADDPLLNARLPGNPKQP
                     LRVVLDPYLKIPVSSRFINSLSEFPGLIICSNSMLKTDKAKVLRQTGAVIHGLDCENG
                     HFNLSEVLNMLHNMNFYSILLETGSGLAEAFLEDNLIDKFIFIYGAKILGGTKSPFPN
                     LGLSNINQAILLSDISYRKIEQDLMVIAYPKVIT"
     gene            complement(5881..7059)
                     /locus_tag="PKM80_01995"
     CDS             complement(5881..7059)
                     /locus_tag="PKM80_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92708.1"
                     /translation="MLKLFKSNRCQSCYNERAMRQCPRKKKNICWQCCNETRCDTHCP
                     DSCPYSPKRDASNPFPAFKADSRAEAENVIKLHIDLWIGRENPMLQNITPEKMAVESP
                     QEMLSWLSGFQYPQYFPMDYLLQKLNLPFSPTSSKEDPETIAGKWMNSLIQLKWDNLR
                     QYTINESSLYDLAERYTEIIKSIPQFSKISTYSMLYSGLGEDGISAIVYMELNHKQDW
                     TLVLSNIKGDWKIRQNIAGNPEAYFKQNKLYSNIAEAIGKADDATAWDLIESNLKIYP
                     DCADLYYYRALYWQMVKQLDQATVDFFNSIALDNDWQEPYLHLSSHYLAKGDYEQAKV
                     WLLELQKMLPDDPNVLNNLAAAYAGNGEFKYAKQLWQEILKKNPAFELARKNLEHLSD
                     "
     gene            complement(7060..7803)
                     /locus_tag="PKM80_02000"
     CDS             complement(7060..7803)
                     /locus_tag="PKM80_02000"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNV92709.1"
                     /translation="MNYRKEIERIVAFIQNYCANTKFTNLLIGLSGGIDSALVAALAV
                     QAIGKKCVIAYNLPHRNSHPESAADALLMANHLDIPLQTIKIDDLVEKYFEHYAPDAS
                     SLRKGNWMARIRMNILYDQAAKHNALVIGTSNRSEYLVGYFTQFGDSACAFEPIAHLY
                     KTEVWEIAREIGLPDKIIQKTPTADLWAGQSDEIEMGITYPVLDSILKYLTEGLPKPD
                     AEEKTIAKVKQMIEKSNFKRQMPPCLERI"
     gene            complement(8741..>9213)
                     /locus_tag="PKM80_02005"
     CDS             complement(8741..>9213)
                     /locus_tag="PKM80_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92710.1"
                     /translation="VFEKYNSAYRFVDNKIIPITNQQEIATIEEAAKTGIKAIDYHLN
                     RAIYIFSNKENKDYVNTVKEAISAVEATVNFINGTKGTTLGDALDKLKEKKSIHPALC
                     DAFKKIYGYTSDKKSGIRHCIFDETDCIPDFTDAKYMLVACSAFINYLLSKAKI"
BASE COUNT         2815 a         1739 c         1510 g         3149 t
ORIGIN      
        1 tgcttcattc ctgatctgac atagttcact tgcagttatt gcattgcgcc tggctaattc
       61 cattaaattt aggatattca ttctgtcaat taaaaataca atctcaaagt agtggataaa
      121 cgccaatctg cagcttcgga acattgttta accatcttaa agaacttatt tgcactaaag
      181 ttatgaaaac ttaccgaatc tatattctca aaattatcaa tgaagggagc aagcttaaat
      241 tcgattaggg aattattatt aaaaaggcaa gcagaattta aattaaaagt taattccttc
      301 caggtacccc aagtatattg aggagagaga gtccagtata aattttttat gtcgtgcctg
      361 aaatcaatac ttgcagtcca ccttctgttt ttagtttgca gttcataata ggattgtttt
      421 tgttgagcgt attgattttc ttcgtatgca aagcccagat caatcataaa agcattttct
      481 ggactattgc gaaaaggaag ttctagccaa cgcggatcta aagttcgagc aattaaagga
      541 aaagtatcga acaccagatt atattgatag cccaattgac ggtaaagagt attattttta
      601 ccagtattgt atatataatc actataatct gctcttattt gatatgtctg gaaaaaagct
      661 atcctgtctc caatcagaaa cttgcattcc ggaaacatag tccagctgct tatctcttta
      721 ttgtttgccg atagcaaaga atctatatac acatctttac taaagctgta accaaaccag
      781 tttcctaaaa ctactcgttc gtgccaataa tgtttccagc caaatcgtaa gttatgggtt
      841 agcaggtcat tatcccattg gttatcatct tcggggaatt tgattatttg caaattaaca
      901 aaataagcag caattaatga atccccttca ctgtattccc aatttatcct gctaccaata
      961 tgtctattat ctgtttgcct tgtattcaaa tcatagtaat agtccttaat agcataagta
     1021 tgagcaagat cgctttgcca cgaaactttt tgcgaaatct gataatctaa gtgtagctgt
     1081 gcctgattat agtgatcagt attattacga atttcacttt tactgtagct tgtttgcctt
     1141 aaactgtaat tatcccgtat ttccaattga acacggtctt caggagaata ttgtagattt
     1201 ccagagatat caaaattcca gcgtaactga ttatccgata gctgataatt actacgaatt
     1261 tcattttgtt ccggagccgc tagaacatag atatcttcgc tatgataatt ataatttgcc
     1321 aggcaggcaa aattcagata atttccgtaa taattatagt tcgctcccaa attagcatag
     1381 tcaaaggatt cccaatccat tcttttgcgt tctgcatttg ctgatatacc tgctatggaa
     1441 tttaaaatat agttagaata acttgcattg gaaccaaacc aatatccctc gctggaaaga
     1501 gaatagccat tcacataacg gtcttgttca tttcttaata tacctttgct gaaaatgcca
     1561 atatttaagc tgtccacagg actgtaattt atatgataac ccaaaatagc agttttattt
     1621 acataagcag caggttccaa atcgctatta tcataaaagg tttgataatc cgaactaata
     1681 gtatgcataa agtgtttact cattaattcc aaacctacat tcaaatctgc atttttggct
     1741 tcctgattaa agtttaaccg attttctatg ttgcttttgc tgttaattac taatgaagtt
     1801 ttagctgtag gataaaatct atactgtaaa gcattttcta tgctgagaac ttgttggctg
     1861 gaatatactg tccagtccaa tccctgagtt tgagctttcc aatatggact acaaagaaga
     1921 aacaagctca gaaagaagaa taaaatcctt ttcttcaagc tcttctcctc tttggcgaaa
     1981 gttgatttgc gacaaattct ccagttcttt ggttttttct tttcccaaaa cagggataaa
     2041 attgttggct agtgtttttc tgcgatgggc aaaagccgcg tctataatgt tattaaaact
     2101 ttctggatat ttaattatgg gtttatcggt tcgagggagt aatagaataa ctgttgaatc
     2161 tacttttggc acaggattga acttctcttt atttacctgt attttgctat aaatatcata
     2221 aaccaaatgc aatcgaatag ttaaaggaga ataatgtttg ctgtgggatt gggcacaaag
     2281 tcgctgagca acttcttttt gaaccataag cacaattcgg gaaaagaatt tagaatgctt
     2341 ttctaacagt gctaaaagcg gactggttat ttgataaggg atattggcta tcaactttaa
     2401 ggggtatcca tcttgttgaa ttaaggaatc ccaattcgcc tgcaggatat ctgtactttc
     2461 cagaacaaat ctcccctgat aatgttcaga aaggtatttc tgcaagcgtt tatcaagttc
     2521 gaaagctctc aattgaacat tccaggatag aatttcatcg gttaatatac ctgtcccggg
     2581 tccaatttcc caaactttat cacctttatg taaatctccc aaagctacaa tttcgcttgc
     2641 gattgtttta tcgattaaaa aattctgccc gagcgcttta agagctttca ttaaagagaa
     2701 aaaatcgctt tcgttctaat tttacaaatt caaaacgaaa gcgaaaacaa gagattacgc
     2761 gcttaatttt ttagcagcca cacttttaca ggttttgcaa accttatatg tcttctcacc
     2821 aactttaact tcattaagta gttttatacc ggttcgatga catacagggc attcacctct
     2881 tccagaatta ggaagttttt ttattccttt gccacgatta tttttcgcca ttatattctc
     2941 cttatgggtt aatatactaa tttttaaaac catatctgaa aaaggtgact tttcagtcaa
     3001 tgcttttttc cgggagtcag aaatctcttg tatctatgac attttccctt tttattattt
     3061 tataagctca aagtgctaag gatgatatta gctaattggc ctataacaat ttatagccgg
     3121 aataagaaca tatatggtaa caacttacaa ttgtaatgag ccctcactgc caaacaaatt
     3181 atcgggaaaa tactagtttc gaatggcagc gaggactcct tgcttttaat gagaacatca
     3241 ttcattcagt tcatcgttta agctcaaaag tatcatctat actcaatacg aattcaacca
     3301 ataactgcac agcattatcc aaatcagcaa gagagataat ctcattagga gtatgcatat
     3361 accgattcgg aatactaagt atggctgttg cggttccttg tctggaggca aaaacagcat
     3421 cagcatctgt tccagttcgg gaaggggaaa tttctatctg aagtggaata ttatgttctt
     3481 ttgctaattc tgtcagtttt ttgtttatcg caggatgcaa agcagaaccc aaacagagta
     3541 cgggtccttt acccaaagaa acatcaccgt aaatgtgttt atccactcca ggaatatcag
     3601 aagtgaaggt tacatctaca gcaacagcaa tatcaggatc aatccgataa gcacttgtta
     3661 tagcacctcg catagttgtt tcttctccga cagaggaaac agcataataa tctgctttta
     3721 tatctttttc ccaaagttct ttcataactg cagaagctat ataaacacct actttgttat
     3781 cggtggcttt acttacaatc agttcttcat tcagttcctc gatttgtgag ctgaaagtta
     3841 tagtatctcc aatagaaaca aggttttggg catcttcttt gtttctagca ccgatatcta
     3901 tccagaggtc ttctaatttc aattttgaag gttcatcact attgcgtaat aaatgaattg
     3961 gcttttttcc aataatacca tgtactatgt tatcgttatg atgaatatct actcttaaac
     4021 caggaagcat atttacatcg aaaccgccta atgctttaac ataaataaag ccgctttcat
     4081 caatgtaatt taccataaat ccgatttcat cagcgtgacc tataatcatt attttatgtt
     4141 taccactgcc ttttttatga gcaatcagat taccattgat atcgatagtt atatcttcac
     4201 agtaatttga aagataagca ttggttagaa cttgagcagg tgtttcaaaa ccagaaggac
     4261 tgtgggtttg caacagatca aacaggtatt ttttattttc gctgttcatt tatgtacctt
     4321 agcagttatt agcttcctca tccgcaaagg gattgagggc tttttttatt accggttttt
     4381 cgtgcgggat taaacagaga aactttaggg tttcttttcc tacattttta aaagtatgtg
     4441 ttaaattggg ctctacgaaa atagcgtcat ctgtgctgaa tgccatttct cccctttctg
     4501 taactattgc accatttccg gaaaggcaat aaacctcatg ttcccattcg tgttgatgaa
     4561 agggggtatg tcctccgggt tcaatttcaa acatacgcat tgcaaaagta ggagcaccat
     4621 ctttttgagc aataagccaa cggatttgtg cgcctttagc tccctctgca tctacgggtt
     4681 ccagttccgc ctcatcgtaa tgaataactt tcattttttc ctccttaagg atttgtgtgt
     4741 tttatagata aactaaagcg aaaagccaaa aaaccaaata cttaagtaat tacttttggg
     4801 taagcaatta ccataagatc ttgctctatt ttacgatagc ttatatcgct tagtaaaatt
     4861 gcctgattaa tattacttag ccccaaattc gggaatggac tttttgtccc tcccaaaatt
     4921 ttggctccat agataaagat gaatttatcg atcaaattat cttctaaaaa agcttcagcc
     4981 aaaccactac ccgtttccaa taaaatgctg taaaaattca tattatgcag catatttaac
     5041 acttctgata aattaaaatg accattctca caatccaaac catgaattac tgcccctgtt
     5101 tgtcttagta cttttgcctt gtcagttttt agcattgaat ttgagcagat aattaaaccg
     5161 ggaaattcgg atagagagtt aataaatctg gaggaaacag gaatttttag gtaaggatct
     5221 aaaacaactc ttagtggttg ttttgggttc ccaggtaggc gagcattcag cagaggatca
     5281 tcagcaagaa tagtttttat tcctgttaat ataacatcat tttcactacg caatttatga
     5341 acatattttc tggctttttc accagttatc cattttgaag agccatcccc ggcagcatat
     5401 tttccatcca acgaaagagc cgtttttaaa gttacaaagg gtctcccttt ttcaatatga
     5461 cataggtaat attccagctg cttttttatt ttgtcttcgt aaaatccgca gtgaaccttg
     5521 atccctgcat tttttagcat tgctattcca tttccagaaa ctatgggatt gggatcagga
     5581 attcctatat aaacttcttg aatgcctgcc tcaattattg cttttgcaca aggaggagtt
     5641 ttcccataat gagagcaagg ttctaaagtt acaaaaatag ttgcaccttc tgcttctttg
     5701 ccagcatgtt taattgcttg aatttcagaa tgatcaccgc catattgtaa agtccaacca
     5761 attgctatca ccttgttatt ttttacaatt acagaaccta caaagggatt gggagagcat
     5821 ttaccccgtg ctttttcagc cgcttgaatt gctaaattaa agtacttatt tatttcaaaa
     5881 tcagtcactt aagtgttcca gattcttacg ggcaagttca aaggcagggt tctttttaag
     5941 aatttcttgc cataattgct tggcatattt gaattctcca ttacctgcat aagcagcagc
     6001 caagttattt agcacattgg gatcgtctgg aagcattttt tgtagctcta gcagccatac
     6061 ttttgcctgc tcataatccc cttttgctaa ataatgtgaa cttaagtgca agtaaggttc
     6121 ctgccaatca ttatccaaag caatggaatt aaaaaaatct acagttgcct gatcaagttg
     6181 ttttaccatc tgccaatata atgctctgta ataatataga tctgcgcaat ccggatatat
     6241 ctttaaattg gattctatca aatcccatgc agttgcatca tctgcttttc caattgcttc
     6301 agctatattg gagtataatt tattttgttt aaaatacgct tcgggattac cggctatgtt
     6361 ttgtcgtatt ttccaatcac ctttaatgtt gctaagcacc aaagtccaat cctgtttatg
     6421 attaagttcc atataaacaa tagcgctaat tccatcttcg cctaatccac tatataacat
     6481 actgtatgta gatattttac taaactgcgg aatagacttt ataatttctg tgtatctttc
     6541 tgctaaatca tatagggaac tttcatttat tgtatattgg cgtaagttat cccatttaag
     6601 ttggattaga gaattcatcc atttcccggc tattgtctct ggatcttctt ttgaggaagt
     6661 gggggagaag ggcaaattca atttttgcaa cagataatcc attggaaaat attgaggata
     6721 ctgaaaaccg cttagccaac ttaacatttc ctgcggcgat tcaacagcca tcttttctgg
     6781 ggttatattt tgcagcatag gattttctct accaatccaa agatcaatat gtagttttat
     6841 cacattttct gcttcagctc ttgaatcagc tttgaatgca ggaaaaggat tactggcatc
     6901 tcttttagga gaatagggac aactatctgg acagtgggta tcacatcttg tttcattaca
     6961 acattgccag caaatatttt ttttcttacg cggacattgt cgcatagctc gttcattgta
     7021 acaggattga cagcggttgc tcttaaacaa tttcaacatt tatatccgct ccaaacaagg
     7081 tggcatttgc cttttaaaat tggatttctc tatcatctgt ttcacttttg ctatcgtctt
     7141 ttcttctgca tccggctttg gcaaaccctc agtaagatat tttaagatgc tatccaaaac
     7201 aggatatgtt attcccattt ctatttcatc actttgccct gcccataaat ctgctgtggg
     7261 tgttttttga atgatcttat ctggcagacc aatttcccgg gcaatttccc acacctctgt
     7321 tttgtataaa tgagcgatag gctcaaacgc acaagcagaa tcgccaaatt gcgtaaaata
     7381 acccaccaaa tattcactgc gattacttgt gccaatcact aaggcattat gttttgctgc
     7441 ttgatcataa agaatgttca ttcggattct tgccatccaa ttaccttttc gtaaagagct
     7501 ggcatcggga gcataatgct caaaatattt ttctaccaaa tcatctatct ttatcgtttg
     7561 cagggggata tcaaggtgat ttgccattaa taaagcatct gcagcacttt ccggatggct
     7621 atttcgatga ggtaaattat aggcaattac acattttttg cctattgcct gaactgccaa
     7681 cgcggcaact aaagcggaat caatcccacc cgataaacca atcagcagat tggtaaattt
     7741 ggtatttgcg caataatttt gaataaacgc aactattcgt tcaatttcct ttctatagtt
     7801 catcttaggt tttactccta ctttgccatt tatcgatgct aattatcctc tttttttcag
     7861 cccgcattcg tcaagcaatt tcttgtttct gcctgtattt tcgtctttca aaccactctt
     7921 ctaccttgtt caatataaac agtacagtaa cacttccacc atatttctgt aacgcgatta
     7981 gagaagtgtt acgaaagtgt gagagagttg ttaggtaatc aactaagttg caatcactta
     8041 tctattatgg gtggtaacag acataattga tggctattta tctgcatatt atagatctaa
     8101 ctgtctatgt cgttatattc aagttgagaa ggttgagagg gtagcaatag cttataaatt
     8161 gagagaaagg aaaatgtcta aacgaaaaaa gataggagaa agtgtaaaaa atattagggg
     8221 agattaaccg ataaatataa ctgcaataaa gagttgtcca attctaaata taaaaaacat
     8281 ctgtgggttt ctgacgaaaa aagatagtta catataaaga agctgaataa tccatcctga
     8341 agtcatttta tcaaatttcc agatacgcaa aataacaatt acagtgtaac aatttttttc
     8401 ccctttaagt atgatagata ataacgacgg attttgaacc cagcgaaaaa acagtccatt
     8461 ctttaaaaaa cataaggttc ccatatacaa tatcaattac cttgtctgcc aaatttatta
     8521 cttcatagtt ttatctttca ttcattacgg tttatttttg ggtaggttat cattctgatg
     8581 tttattgtga cattcttgtt ttaactctca attttgaaat agataaacca gttaatacga
     8641 gtatgcctta ctgtaaattt taggaaaagt tacattagat caatacatat caaaattgaa
     8701 agtcaaaaca agcagttttc agatgcaaca atctatcaga ttatatcttt gccttagaga
     8761 gtaaataatt tataaaagca gaacaagcca ccaacatata tttagcatca gtaaaatctg
     8821 gaatacaatc tgtttcatca aatatacaat gtcttatacc cgatttctta tctgaagtat
     8881 atccatagat ttttttgaag gcatcacata aagcaggatg aatggatttc ttttctttta
     8941 atttgtccaa agcatcacct aaggtcgttc ctttagttcc attgataaaa ttaaccgttg
     9001 cttcaactgc agagatagct tctttaacag tattcacata atctttattt tccttattgg
     9061 aaaatatata aatagccctg tttaaatgat aatctattgc ttttataccc gtttttgctg
     9121 cttcctctat tgtagctatt tcttgctgat tcgtaattgg aattatcttg ttatcaacaa
     9181 aacgatatgc agaattgtat ttttcaaaaa cag
//
LOCUS       DAORHB010000036         9213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_6929_length_9213_cov_4.573815, whole genome shotgun sequence.
ACCESSION   DAORHB010000036 DAORHB010000000
VERSION     DAORHB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6929_length_9213_cov_4.573815"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..571
                     /gene="secG"
                     /locus_tag="PKM80_02010"
     CDS             233..571
                     /gene="secG"
                     /locus_tag="PKM80_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNV92711.1"
                     /translation="MVLYSIALIIHVIICIALVLVILAQTSKGGLDSNLGGAAMNVFG
                     GSGASKFLKKWTQILAVVFAASCVLLAFLVRDINSSALTGIQERQKKIKDTEQQAPAA
                     NPIPKQVPTK"
     gene            576..663
                     /locus_tag="PKM80_02015"
     tRNA            576..663
                     /locus_tag="PKM80_02015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:610..612,aa:Leu,seq:aag)
     gene            810..1259
                     /locus_tag="PKM80_02020"
     CDS             810..1259
                     /locus_tag="PKM80_02020"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNV92712.1"
                     /translation="MKRRILALITLTISGCLLAQPVNKDPTKAVIYSLFPGGGQIYNE
                     AYIKAGAVIGFQSYLLATAIHNNDKVNDYKDKISITTDPILIESYKSKQKNYKEKRTR
                     DIWLMGLTMAFSTLDAYIDAHLSNFNSDKEKVHLRFEKDILLLEYEF"
     gene            1275..2057
                     /locus_tag="PKM80_02025"
     CDS             1275..2057
                     /locus_tag="PKM80_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNV92713.1"
                     /translation="MRKFLLLLILLLLSCTQSSKTISISQFEQRIWELTNQERIAHNL
                     SPLLYDEGLADLARMHSSNMFKYDFFAHKDHLGYLVDDRKRRYYPQLIVASIGENLAK
                     IVSGAKIFTPEEIVEGWMNSTEHRENILNGSFTHLGVGVFTQGSVLYATQNFGTPIAK
                     LLSQIPEQLEVKNKYQLQFEFMSLEPVNELEAILNFPESNFKYNIDDQYYTLGLKPIP
                     IHWITDKQFEVIIDFPAGKGDYNLCFGFNRSYFADGIKLKVK"
     gene            complement(2082..2852)
                     /locus_tag="PKM80_02030"
     CDS             complement(2082..2852)
                     /locus_tag="PKM80_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNV92714.1"
                     /translation="MKLTLWSWNVNGLRAALNKGFIKIIQNEQPDILGIQETKLQEHQ
                     IPEELNTLANYLIYWSHSIRKGYSGTGLFTKILPISFSTSFGNPEFDSEGRINIAEYD
                     KFIYFNIYFPNGQKDDERLNYKLRFYDRCLEMMEEKRTTGKIILVGGDFNTAHKPIDL
                     ANPKENEKTSGFLPIERAWLDKIETMGWVDTFRHFDKSPEQYSWWSYRTKARPRNVGW
                     RLDYFWVNQEGLKHIINAGIRQDIEGSDHCPVFVELEI"
     gene            complement(2862..3503)
                     /locus_tag="PKM80_02035"
     CDS             complement(2862..3503)
                     /locus_tag="PKM80_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92715.1"
                     /translation="MPSCKATLYSLISILILLSLTACKIDMKQEIWLKKDNSGKASVI
                     VHMQIPFGLQNETLDLQELNSFNALSSLAEVARKTPGVSVTRLAQEETHNKKEMNLSY
                     YLDFTFKDVEGLSTVLSAEGRNAIFLTKSGKHKVLCIKAEELALIEDDDSDEFDFLSF
                     IEINLRNKVNLPARVKKIDNTGYGKYKGKTATWDIELDDDWLSCTSHLLYVHF"
     gene            3618..6041
                     /locus_tag="PKM80_02040"
     CDS             3618..6041
                     /locus_tag="PKM80_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNV92716.1"
                     /translation="MKNTLIRLFLVSLLMLIMLPICAQSEAISPAMPDTLFPEIPDSL
                     DINIVKTDSLFYKADSIHFEYTTEIIKLYGKPQINYQDTEIIADSITIDIKNECAYSY
                     GPTRMKDGDQLLLGTNIRFDVNTQTGILDDGKSLIEGGYYSGQELRKTDKDTYDIDGG
                     TFTNCDLEEPSFWFWAKQLRVYKGDKIVGKPVIGYVNHMPVFFFPFITIPIRQGRYPG
                     FLIPSPDYNNVDGFVIRNLAVYYPYKDYADFVFGFDIMEKTGWKGHFDTSYLKRYSFS
                     GNLNASYQHNLKDDVTYDDWSLQGRHHQDLPEKSSLDANINFVSNKRIWESSSNVDQS
                     LAQRLTSSVSYSKPIGSSYFNAGSNFTQDLINDTATLSLPSASFYLASRPVSELLNVS
                     SDSWLSNFNYRYNAYLEHTGYLKEKNYSISDLFWDNTVDPSDTTGLTMLNEHHFGIKH
                     NAGISRNSKLFGWLTLGESFDYSEAWMDRDKQNDKYARGNAWTASVNARFNLYGLRNF
                     SNFPVTSVRHIITPNIGYSYQPDTRKNSDLYNFGSIAVSSVKESSNLNFSVDNNWQLK
                     YGTKGKETKLNDLLYWRIGTSANLKKKDQPFSNISHNLTFKPGTYKIGTLSINDGKYQ
                     VKDIKVGYSSQFSATQNPYQIHWNDMNLRNQYFSQGISISGSAPYTKYFVAPKNRNFE
                     VFESYDSLNAYPKSFQEDTGSNDWSFSISQNIYADKDIFHSESSNLRMNLTCKITENW
                     SLAYTNYYNLKTSNMLAQTLSISRDLHCWKMDINITLRNDYWDYRITLFNTLLPDALR
                     FQTHDSRKY"
     gene            6022..6612
                     /locus_tag="PKM80_02045"
     CDS             6022..6612
                     /locus_tag="PKM80_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV92717.1"
                     /translation="MTAENIKSAIFLDRDGVISPDDFGYISEPEQYHLYPYTIEALRI
                     FKELDFLLFVVTNQSGIARGYFSIEDLERVLAKMRSLISAGGIELDGVYYSPYYKDGI
                     IPPYNIDHEDRKPGIGMFKKAYSDFHFDIEQSWMIGDRHSDIAFGKNAGLKNILLLTG
                     NGHKDFIEIIKTNELKPNFVCENLLTAANLIRDFKL"
     gene            6609..7418
                     /locus_tag="PKM80_02050"
     CDS             6609..7418
                     /locus_tag="PKM80_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV92718.1"
                     /translation="MKFSACYSATKFKAFTYEEQIKALHKLILCLEQNLSNPEKRLSI
                     IDHLLKLSQLVDEPLPQKMHFFLKELNPRLSPHQLMNKLYFYLEGSLRKDGSMSLRIG
                     DGKISADPFLRKKAKQITVICDNLRSVFNVGSLFRTSECLGIGKILLCGITPTPEHSN
                     MPKTAMGTEKLVEWQYLQTSIDAITICRESGMYIYALETVEEAGSIFQTIYKLPLAIV
                     IGNEALGIEPSVLELCDECIALPQLGWKNSLNVGVATGIALYQIIFGGPNG"
     gene            7411..8208
                     /locus_tag="PKM80_02055"
     CDS             7411..8208
                     /locus_tag="PKM80_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HNV92719.1"
                     /translation="MDNLTFYEKISIFLKEHKTVWQASIIESDGSTPAKCGMKLAVTL
                     NEEPFGNLGGGDLEHTVIEMIKKEKPRTAQIFSFNLSTDGAKTGIATSMLCGGQVKIY
                     IEPLFLSDKLYIIGAGHCGKALGKLARLCGFYVYLIDNRKEIIDNLLEDCYHTAILHN
                     YTDLSEIIDFDSDSWIVIMTQGHLHDKEVLEQCLRTNFRYLGMMGSKKKVLDTFAKLK
                     EEGFTEKELFRCHSPIGLPIGSNTPEEIAVSIMAELISIRNSKNNER"
     gene            complement(8387..9022)
                     /locus_tag="PKM80_02060"
     CDS             complement(8387..9022)
                     /locus_tag="PKM80_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNV92720.1"
                     /translation="MNKKDSTYLIILPDCVIKHFNDPKHFQAELYAYQAGLRMLPDLL
                     DFQVPQWIKIEKVKGIPYLDRLDYFYPELLAETYANFHLATLKNDKCLCHVDNSPKNI
                     LFCQGKYYFIDFADSRFEYPERDITHLLLFWAADFPADYFKYALETFLNGYLSFVTLN
                     SERWNSYLKSNIILFDQRRKLHSKKTGKESPSIQNLNRELLAQMKLCYMKE"
     gene            complement(9015..>9213)
                     /locus_tag="PKM80_02065"
     CDS             complement(9015..>9213)
                     /locus_tag="PKM80_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92721.1"
                     /translation="IDDLGRIYISSWGQNMIFEIPQEQNRFLATFTDIEDAADILYYL
                     PNNELIVPLFNKNKIIRLKLE"
BASE COUNT         2989 a         1537 c         1660 g         3027 t
ORIGIN      
        1 tacaacagtg tgttataact tgtccacatc acttttttgt gggaatgcct tgaaaaatat
       61 gtctatttct atttatcgct tttccatatg gtaataatgt atataataac tataaatgag
      121 cactcaagca agtttcaatt tctatgctac cgattatttt gattgacaga aagaggacat
      181 acaatttttt gtaaaattct aaaaagatta taaggttaat aaggactaaa taatggtttt
      241 atactcgatt gccttaatta tccatgtgat aatttgtatt gccctagtat tggtgatttt
      301 agcccaaact tccaagggcg ggttggattc caatttaggt ggagccgcaa tgaatgtttt
      361 tgggggcagt ggtgcctcta aatttctaaa aaagtggact cagatacttg ctgttgtttt
      421 tgctgcttcc tgtgtgctgc ttgctttttt ggttagagat ataaacagca gtgctcttac
      481 aggaattcaa gaacgccaaa aaaagattaa agatacggaa cagcaagctc ctgcggctaa
      541 tccaattcct aaacaagtac ctactaaata aataagcaga agtggtggaa ctggcagaca
      601 cgcaagacta aggatcttgt gcctgtaatg ggtgtgcggg ttcgagtccc gccttctgca
      661 ccatttttta tattaggagc tttaacagct cctttttttt atggagtgaa agaatccctt
      721 tggttgcact tttttattct ccagcatatt gttattgaca aaatagtgga ataagaagaa
      781 aaagccaaaa aagctaaagg ggagttatct tgaaacgaag aatattagcg ttaatcacat
      841 taaccataag cggttgcttg ttagctcaac cggttaataa ggatcctacc aaagctgtta
      901 tatattctct atttcccgga gggggtcaga tttacaatga agcatacata aaagctggag
      961 ctgtaattgg atttcaaagt tatttgctgg ctactgccat tcataataat gataaggtta
     1021 acgattataa ggataaaatc agcattacta ctgatcctat actaatcgaa agttacaaaa
     1081 gcaaacaaaa aaactataaa gaaaaacgca cgcgtgatat ttggttgatg gggttaacta
     1141 tggctttttc cactctcgat gcatatatag atgcacatct atcaaatttt aattctgata
     1201 aggaaaaagt acatcttcgt tttgagaaag atattcttct tttagaatat gaattttagc
     1261 ttgatcgagg atagatgaga aaatttctat tacttcttat attattactt ttgagctgca
     1321 ctcaatcgtc caaaaccatt agtatttctc aatttgaaca aagaatatgg gagctgacta
     1381 atcaggaacg aatagcacat aatctttctc ctcttttata tgatgagggt ttggcagatt
     1441 tggcccgtat gcacagtagt aatatgttta aatatgattt ctttgcacat aaagaccatc
     1501 ttggctatct tgttgatgat cgtaaaagaa gatactatcc gcaattaata gttgcctcta
     1561 ttggcgaaaa tttagctaaa atcgtgagcg gagcaaaaat ctttactcca gaagaaatag
     1621 tagagggttg gatgaattct actgaacata gagaaaatat cttaaatggc tctttcactc
     1681 atttaggagt tggtgtgttt acccaaggga gtgttttgta tgctactcag aattttggaa
     1741 cacctatagc taagttgctt tcgcagattc ctgaacagct tgaagtgaaa aataaatatc
     1801 agctacagtt tgaatttatg tctctcgaac cggtaaatga attagaggca atactaaact
     1861 ttcctgaatc taatttcaag tataatatag atgatcagta ttataccttg ggattgaaac
     1921 ctattccaat ccactggata accgataaac aatttgaagt aataatagat tttcctgcag
     1981 ggaagggtga ttacaatcta tgttttggtt ttaaccgttc ctattttgca gatggtatta
     2041 aattaaaagt aaagtagata agcacagttt aaatatacag cttatatttc cagttctaca
     2101 aatacgggac aatggtctga accctcaata tcctgtctga tccctgcgtt tataatatgt
     2161 ttcagacctt cttgattcac ccaaaaataa tcgagacgcc atcctacatt tcttggtctg
     2221 gcctttgttc tatagctcca ccaactgtat tgttccggac ttttatcaaa atgacggaaa
     2281 gtatctaccc agcccatagt ttcgatttta tccagccagg cacgttcaat aggtagaaat
     2341 ccactagttt tttcattttc tttcggattg gctaggtcta tgggtttgtg agcagtatta
     2401 aaatctccac ccactaaaat aatcttgccc gttgttcttt tttcttccat catttccaga
     2461 cagcgatcat agaaacgcag tttataattt agccgttcat catccttttg tccgtttggg
     2521 aaatagatat tgaaatagat aaatttatcg tattcggcaa tgtttattct accttcgctg
     2581 tcaaattccg gattgccaaa actagtggaa aagctaatcg gtaatatctt tgtaaataat
     2641 cctgtgccgg aatatccttt tcggatacta tgagaccaat aaataagata gttcgctaaa
     2701 gtatttagtt cttcaggaat ttgatgttcc tgaagttttg tttcctgtat tcctaagata
     2761 tccggttgtt cgttttgaat gattttaata aaacctttat ttaaggcagc tcgcaatcca
     2821 tttacattcc aagaccagag tgttaatttc aaaaatcccc cttaaaaatg aacatataat
     2881 agatggctgg tacaactaag ccaatcgtca tctaactcaa tatcccaagt agcagtttta
     2941 cccttatatt tcccataacc tgtgttatct attttcttta cccttgccgg cagattaacc
     3001 ttatttcgca aatttatttc gataaagcta agaaaatcaa attcgtcaga atcatcatct
     3061 tcgatgaggg caagttcttc tgcttttatg catagcactt tatgcttgcc tgattttgta
     3121 aggaatatag catttctccc ttccgcgctc aaaactgtgc tcaacccttc tacatcttta
     3181 aaggtaaaat ccagataata agacaaattc atttctttct tattatgagt ttcttcttga
     3241 gctaatctgg taactgatac accaggtgtt tttctggcaa cttcggcaag tgaagataga
     3301 gcattgaaag aattaagttc ctgcaagtcc agggtttcat tttgtaggcc aaaaggaatc
     3361 tgcatatgta ctataacaga tgcctttcct gaattatctt ttttcagcca aatttcctgt
     3421 ttcatatcaa ttttacaggc agttaagctt agcaaaatca ggatacttat caaagaatat
     3481 aatgtagctt tacaggaagg catttgtatc tcctttaaaa aaagcgtttt ttatatatat
     3541 taattggctt tggcttaaag gcaaaaatta ttatcccgct tgacacatag atagctctgt
     3601 ttttttttga ttacaaaatg aagaataccc taatccgttt atttctggtt tccttactaa
     3661 tgctaataat gcttcctatt tgtgctcaaa gtgaagctat tagccccgct atgcctgata
     3721 cgctgtttcc tgaaattccg gatagcctag atattaacat agtaaagaca gattctcttt
     3781 tttataaagc ggatagcatc cattttgaat atacaacaga aataataaaa ctctatggta
     3841 aacctcaaat taattatcag gatacagaaa ttattgccga ttctatcaca atagatatta
     3901 aaaacgaatg tgcttatagc tatggtccaa cccgaatgaa agatggcgac cagttacttc
     3961 ttggtaccaa tattcgcttt gatgttaata ctcaaaccgg tattttggat gatggcaaaa
     4021 gtttgataga aggtggttat tatagcggac aagaactccg taaaaccgat aaagatactt
     4081 atgatattga tggaggaaca tttactaatt gtgatctgga ggaaccttca ttctggtttt
     4141 gggcaaaaca attacgggtg tataaaggcg ataaaatcgt tggtaaacca gttataggct
     4201 atgtaaatca tatgccagta ttttttttcc cttttatcac gattccgatc agacagggaa
     4261 ggtatcctgg ctttttaatt ccttcgccag attataataa tgtcgatggc tttgtaataa
     4321 gaaatcttgc tgtttattat ccatacaaag actatgcaga ttttgttttt ggttttgata
     4381 ttatggagaa aactggctgg aaaggtcatt ttgataccag ttatctaaaa cgctattctt
     4441 tttcaggtaa tttgaatgct tcttatcaac ataacttaaa agatgatgtt acttatgatg
     4501 attggtcttt gcagggtaga catcaccaag atcttccaga aaaatcatct ttggatgcca
     4561 acatcaattt tgtaagtaat aaaagaattt gggaaagtag cagtaatgtt gaccaaagct
     4621 tagctcaaag attaacttcc agcgtttcgt attccaaacc gattggttct tcttatttta
     4681 atgcaggtag taattttact caggatttaa taaatgatac agctacttta tctttaccaa
     4741 gtgcctcatt ttatttagca agccgtcccg tatcggaact tttaaatgtt tcatccgatt
     4801 cttggcttag caatttcaac taccggtata atgcttatct tgagcataca ggatatttaa
     4861 aagaaaagaa ttactctata agcgatttgt tttgggataa tacggttgat ccttcagata
     4921 ccacaggact aacaatgcta aatgagcatc attttggtat aaagcataat gcagggattt
     4981 cgcgtaattc caaattattt ggttggttaa cattagggga aagttttgac tattcggaag
     5041 cttggatgga tagagataaa cagaatgata aatatgccag aggaaatgcc tggactgcat
     5101 ctgtaaatgc cagattcaat ctttatggat taagaaattt tagtaatttt ccagtaacat
     5161 ctgtccgtca tattattact cctaatatcg gttattccta tcagccggat acgcgaaaaa
     5221 actctgattt atataatttt ggcagcatag cggttagcag tgtaaaggaa tcatctaatc
     5281 taaatttcag tgtggataac aattggcagc ttaagtatgg aacaaagggt aaagaaacaa
     5341 agctcaatga cttgttatat tggaggatag gtacatctgc aaatcttaag aagaaggatc
     5401 agcccttttc taatatttcg cataatttaa ctttcaaacc agggacatat aaaataggca
     5461 cgctttctat caatgatgga aaataccaag ttaaagacat aaaagttggt tattcatctc
     5521 aatttagcgc tactcagaat ccctatcaaa ttcactggaa cgatatgaac ttgagaaatc
     5581 agtatttttc tcagggaata tcaattagcg gttcagcacc ttatactaaa tattttgttg
     5641 ctcccaaaaa cagaaatttc gaagtatttg aaagttacga ttcgttaaat gcatatccaa
     5701 agagctttca agaagatact ggttccaatg actggagctt ttccatcagc caaaatattt
     5761 atgccgataa ggacatattc cattctgaaa gtagtaactt aagaatgaac ttaacctgta
     5821 aaattactga gaactggagt ttagcatata ccaattatta taatttaaaa accagcaata
     5881 tgcttgctca aaccttatct atttccagag atctgcattg ctggaaaatg gatattaata
     5941 ttactttaag aaatgattat tgggattata gaattacact atttaacacc ttattaccag
     6001 atgctttgag gtttcagacc catgacagca gaaaatatta aaagtgccat ttttttagac
     6061 cgagatggag ttatcagtcc cgatgatttt ggatatataa gcgagcccga gcagtatcat
     6121 ctttatcctt atactataga ggcattaagg atatttaaag aattggattt tttgcttttt
     6181 gtggttacta atcagtccgg aattgcaaga ggttattttt ctatagagga cttagaaaga
     6241 gtattggcaa agatgcgttc tttaatttct gctggaggaa tagaactgga cggagtatat
     6301 tattctccct attataaaga tggtattatt ccaccatata atattgatca cgaagatcgt
     6361 aagcctggaa ttggaatgtt caaaaaagca tattccgatt ttcattttga tattgaacaa
     6421 tcttggatga ttggagatcg ccatagtgat attgcctttg ggaaaaatgc gggactaaag
     6481 aatattctct tgttaacggg aaatggacac aaggatttta tcgagattat aaagacgaat
     6541 gaacttaaac ctaattttgt ttgcgaaaat ttacttacgg ccgccaatct gattagggat
     6601 ttcaagttat gaagttttct gcttgttaca gtgcaacaaa atttaaagca tttacatatg
     6661 aggaacaaat aaaagcgcta cataaactga tattgtgctt agagcagaat ctaagcaatc
     6721 ctgaaaagcg tctttccata attgatcatc tgctaaagct ctctcaacta gtagacgaac
     6781 ctttaccaca gaaaatgcac ttttttttaa aagaactgaa tcctcgctta agtccacatc
     6841 agcttatgaa taaactctat ttttatctgg aaggatcttt aagaaaagat ggttcaatgt
     6901 ctctaagaat cggggatgga aaaatatcgg cagacccttt tctccgcaaa aaagcaaaac
     6961 aaataaccgt tatttgtgat aatttgcgtt cggtttttaa tgtgggttcc ttgtttcgaa
     7021 cttctgagtg tttaggaatt gggaaaattc tgttatgcgg aataactcca actccagagc
     7081 atagcaatat gccaaaaaca gctatgggaa ctgaaaaatt ggttgaatgg caatatttac
     7141 aaacatctat tgatgcaatt actatatgtc gtgaatctgg tatgtatata tatgccttag
     7201 aaacagtaga agaggcaggt agtatatttc aaaccatcta taaactccct ttggctattg
     7261 ttatcggtaa tgaagctctg ggtatagaac cttcagtttt agaactttgt gatgaatgca
     7321 ttgctttgcc tcaattggga tggaaaaact ctttgaatgt tggagtggca acaggaattg
     7381 ctctctacca gattattttt ggaggtccaa atggataatc ttacttttta cgaaaagata
     7441 tctatctttc taaaagaaca caaaactgta tggcaggcaa gcattattga aagcgacggt
     7501 tcaactccag ccaaatgtgg catgaaatta gccgttactc taaatgaaga accttttggc
     7561 aatctgggtg gaggggattt agaacacact gttatagaga tgattaaaaa agaaaaaccc
     7621 aggactgcac aaatattcag ctttaactta tctacagatg gggctaaaac aggaattgcc
     7681 acttctatgc tttgcggagg acaggttaaa atatatatag aacccttatt tttaagtgat
     7741 aaactctata tcattggagc aggacattgc ggaaaagcat taggtaaatt agctcgactc
     7801 tgcggattct atgtttatct gatcgataac cgcaaagaaa ttattgataa tttacttgag
     7861 gattgttatc acactgctat tcttcataat tatacagacc tttcagagat tatagatttt
     7921 gattcggata gctggattgt tattatgact caaggtcatt tgcacgataa agaagttttg
     7981 gaacagtgct taagaactaa cttccgttat ttaggaatga tgggtagtaa aaaaaaggtg
     8041 cttgatactt ttgccaaatt aaaagaagag ggctttacag agaaagaact tttccgttgc
     8101 cattcgccta taggtttacc tattggctca aatacccctg aagaaattgc tgtaagtatt
     8161 atggcagaac tgatctctat tcgcaattct aaaaacaacg agcgttgaat atttagtttc
     8221 ttattgtagt ttttcagtta aaagccaatt cttgattcta ttgatacgag gttaatttag
     8281 caaaaatata attattaccc tgtttttcta ttcgaagctg aagcttaggt tattttaaaa
     8341 taaacgaaat atcgatccga tatgggggaa aatcataaaa acaagttcac tctttcatat
     8401 aacacagctt catttgggct aaaagttccc ggtttaaatt ctggattgaa ggggattctt
     8461 ttccggtttt tttgctgtgg agttttcttc tttggtcaaa aagaataatg ttacttttta
     8521 agtaagaatt ccagcgttca gaatttaagg ttacaaaaga gagatatcca ttcaaaaaag
     8581 tctcaagcgc atacttaaaa taatcagcag gaaaatcggc agcccaaaaa agtaaaagat
     8641 gagtaatatc cctttctgga tattcaaatc tactatcagc aaaatcgata aaataatatt
     8701 ttccttgaca gaacaggata ttcttaggtg aattatcgac gtggcagagg catttatcgt
     8761 ttttaagtgt agctaaatga aagttagcat aagtttcagc aagaagttca ggataaaagt
     8821 aatccagtct atccaaatag ggaatgccct ttactttttc aattttaatc cattgcggta
     8881 cttgaaaatc taaaagatca ggcaacattc tcagtccagc ttgatatgca taaagttctg
     8941 cctgaaagtg cttcgggtca ttaaaatgct tgatcacaca atcaggcaga ataattagat
     9001 aggtagaatc ttttttattc aagctttaag cggatgattt tattcttatt gaacagagga
     9061 acaattaatt cattattcgg cagataatat aaaatatcag cagcatcttc aatgtccgta
     9121 aatgtggcta aaaagcgatt ttgttcttgg ggaatctcaa aaatcatatt ttgaccccaa
     9181 ctgctaatat aaattctgcc taaatcatca ata
//
LOCUS       DAORHB010000037         8891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_7301_length_8891_cov_4.024219, whole genome shotgun sequence.
ACCESSION   DAORHB010000037 DAORHB010000000
VERSION     DAORHB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7301_length_8891_cov_4.024219"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            603..3179
                     /locus_tag="PKM80_02070"
     CDS             603..3179
                     /locus_tag="PKM80_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNV92722.1"
                     /translation="MKKCTCLLLCVIPILGFAAPFYNIPSQAIQPDGSVLNLYASGDE
                     YSNRLHDANGFTIIQSPDDGYYYYAILENGEPVPSAYRFGTIDPASYGITPNINISKG
                     ARQRKINFMNAHKRGNERGPSTGVVNNLCVYIRFSDQTEFEIPRTVYDARFNEVGDDA
                     RSLRSYFQKASYNQLHYMTYHYPVCPDIFNLSYQDSHPRSYYLPYNYITNPGGYHNED
                     ERCIREQTLLMNAINSIASQVPSSLNIDADNNGTVDNVCFIIRGPHSAWADLLWAHRW
                     SLFYTSAYINNKEVYDFTFQPEDQNDVTTLCHEMFHSVGAPDLYHYEYDGLTPVGCWD
                     LMESGDGHMGMYMKYAYGGWIPSIPLAQIGNTYTLNPVTSAENNVYKYPIPGSTNQFL
                     IFEYRKKSSDIFEENLPNSGLLIYRIDQTMDGNVEGPPDGVYVYRPDGSLTNNGLVAE
                     APFSANNFRTAFNEYTNPSSFLNNGSTFPVNITNITEAGETISFTIFSPAVTMAPVID
                     AIHPANGSILADNTLNIYLQVTDPAEAIQRVEYTLDGILVYTATAEPYTGIISDYLLT
                     PGVHNIGITAFSASNLSTTVNINYRIVNPDLENWFSWLSSEPHWIEYGRGAIPIKAAI
                     DMDLGNQEYIVKALRFNITPDPWGEPDMPGMVYARINRFSNGAITDQLLLDIGYCYNF
                     DYDPNFTFNVVDTTHISGQIAVILDLYEYQNMRFDQNAACGHSWIYEPNRPWTDALGR
                     GIVGAAAIELLLQNPNVSIEEPNCPALNISLSSYPNPFTSTTKIKYSLPASGKVNLTI
                     YNLKGQKVKNLLTENKTVGNYEMEWNGTDTQGKNVGSGLYLCRLVSGNRTATTKLLRL
                     SD"
     gene            4015..5358
                     /gene="gcvPA"
                     /locus_tag="PKM80_02075"
     CDS             4015..5358
                     /gene="gcvPA"
                     /locus_tag="PKM80_02075"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNV92723.1"
                     /translation="MPYISNTDNDRRKILKVIGANKFEDLISAIPAKLRMCNPLAISE
                     PLSELEINRNIQTKADKNKPCIKVNSFLGGGVYDHFIPAAVDAIVSRPEFFTAYTPYQ
                     AEVSQGTLQYIFEYQTMICELTGMEIANAGMYDAASSIAEAILMAVRKNHLTKAILPA
                     TLHPNYVKVIKAYTEGIGIELLTVPEKEGVTDLSALEAMMDETIGSVVLQTPNFYGNL
                     EDAKAIDTIVHKNSKCLLIASVDPVSLAVLIPPSEYNADIAVGEGQALGNNMYMGGPL
                     FGFFATKLDMARLMPGRIVGGTIDKAGEKAYVLTLQAREQHIRRAKATSNICSNESLC
                     TLAAVVYLCLLGKEGLIEVATQSVQKAHYAASELCKIPSLALAYPKATFFKEFVIRTP
                     KKAADIINALLPENIYAGIDLAPYGKPNELMIAITEKKSKTEIDSFVQAMKEVVK"
     gene            5355..6809
                     /gene="gcvPB"
                     /locus_tag="PKM80_02080"
     CDS             5355..6809
                     /gene="gcvPB"
                     /locus_tag="PKM80_02080"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNV92724.1"
                     /translation="MSKTIFEHSSPSRKGVTLPKREIDTPLPTLIPETMLRTKKVRLP
                     EVSELDVMRHYISLSHKNHFIEKGLYPLGSCTMKYNPKLNETLVRHSSFTTLHPFQPE
                     ETMQGSLELMYELQNDLAEISGMKGVTLQPSAGAQGEFTGIKIIAAYHKAKGNNHKDT
                     IIIPDSAHGTNPASCSLVGYKTVELASNKEGRCDIAKLRELVNENTAGIMITNPSTLG
                     LFETQIEEIAEIIHSVDALMYMDGANLNALLGIVQPGKIGFDLMHFNLHKTFATPHGG
                     GGPGSGPVAVNEKLLPFLPVPIVTKDECGYHLSYENEATSIGKVHSFYGNFAVLIRAY
                     IYIKMLGAEGLRRVSENAILNANYLMKILGDYYHIEHQENCMHEFVMDGTRQKKEYGI
                     TTLDIAKRLLDKGFHSPTIYFPLIIPEAMMVEPTETECLESLDAFAKAMIEIAEECKE
                     NPELVKEAPLTTPVKRVDDVRAVKVLEPIFPLPN"
     gene            6840..7466
                     /locus_tag="PKM80_02085"
     CDS             6840..7466
                     /locus_tag="PKM80_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNV92725.1"
                     /translation="MKKHILVLIVLVASFALAYAQPVKLGFVNTDRLLLESNEAAEVA
                     RLFALDKQNWTNQIRTLDDEIKQMERDFEIRKLTMNDAAKRETQSRIDTKKTEAGRLL
                     EEYFGDGGKAEQRYRELIDPLTLKIHNLIKKIAEDEKYTMIFDVSMGSLLYALPTLDL
                     TEQILLELNKDTVKPTDVSPTDINPPKDQTPPEIKSPEGYEEFKPDKK"
     gene            7463..8512
                     /gene="lpxD"
                     /locus_tag="PKM80_02090"
     CDS             7463..8512
                     /gene="lpxD"
                     /locus_tag="PKM80_02090"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNV92726.1"
                     /translation="MKRFSTNISEEILLHICQGELIGKIPENLCKVSEPQEADQQTII
                     FLEQEKLLDIVKASSAGLIITTKRYVPMFPDRALLIVEKPYFALMLLITYLLNQENKN
                     ITYTIHPSAVVAEDVILDKEVAIGANVVIGSGSKLGKGVIIGEGCSIGKNVYIGERTK
                     LYPNVCLYDDCVIGNNCILHSGVIIGADGFGFMLIAGVQQKIPQVGNVVIGNDVEIGA
                     NSCIDRATLGSTIIGNNTKIDNLVQIGHNCVVGEHSVICSQVGLAGSTIVGDFVYLAG
                     QVGVADHLKIGNRAMIGAQSGVSSNIPDDGKYFGYPALDANLTKRIMAVQKNLPEMYF
                     AYLKAKKKETNSGDK"
     gene            8512..>8891
                     /locus_tag="PKM80_02095"
     CDS             8512..>8891
                     /locus_tag="PKM80_02095"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNV92727.1"
                     /translation="MEYKQTIGGESSYTGIGLHTGEISTIRFKPAGPEEGIVFIRTDL
                     PGKPEIPADIDHVVDISRGTTIGINGATVATIEHVLSAVVGMRIDNIRIEINGPEAPV
                     ADGSAVVFLNILRQCGIMQQDSER"
BASE COUNT         2829 a         1554 c         1729 g         2779 t
ORIGIN      
        1 catcgacaac gaataacttg gtttttgtta ataagcctct atccttaatt gtcatcttga
       61 tattatcttg gacgatactc tttctctact cgttcctctc tcctcctaat cgaggagtat
      121 aggaggagag aaggataggt ggatgaggag aggcaaaaag tgaggcatcc tggaaaaagt
      181 ggacttaacc caagaagtaa gaaatgcaca aaaagtttat tgaaaggttg catattcagg
      241 atagtgattt cttcgaactt gagattgcta acatttgagt aatctttggt gagttatttt
      301 tggtgacttc ggtggctaaa tattcttcta aaaaatcttg tttatagaga taaattctct
      361 taatcctaaa atcctaaaat ccttgtttcg aaaactaaat ctgcgtgagt tatttccggg
      421 aagaacgaca tcctgtcgtt cgatggtctt tatctgcaaa ttttcgttgg tttttttaac
      481 aggaaaaagc aaaattcagt ctgctatttt aggactgaac agttttaaca tattgttttt
      541 atgctttttt gattgcttta ggtacagatc ttgcttacta tttatatata aggagatgaa
      601 ttatgaagaa atgtacttgc ttgttattat gtgttatccc tattttggga tttgctgccc
      661 ctttttataa tataccttca caagcgattc aacctgatgg ctctgttcta aacttatatg
      721 ccagtggtga tgaatattct aatcgtttac acgatgctaa tggttttaca ataattcaaa
      781 gtccggatga tggatattat tattatgcaa tcttggaaaa cggtgaaccg gttccttcag
      841 cttatcgttt tggaacaatt gatcctgctt cttatggtat aactcccaat attaatattt
      901 ccaaaggagc caggcagaga aaaattaact ttatgaatgc tcataaaaga gggaatgaac
      961 gaggccctag cactggtgta gtaaacaatc tatgcgtata tatccgtttc agcgatcaga
     1021 ccgaattcga gattccgcgc acagtttatg atgcccgttt taatgaggtt ggcgatgatg
     1081 ctcgcagttt acggagctat tttcaaaaag caagttataa tcagttgcat tatatgactt
     1141 atcattatcc cgtatgccca gatattttta atctttcata tcaggatagc catccccgta
     1201 gttattatct tccatataat tatattacaa atcctggtgg ttatcataat gaagatgaaa
     1261 gatgtatccg tgaacagacc ttgcttatga atgccataaa ttctattgct tcgcaagttc
     1321 cttcttcttt aaatattgat gcggataaca atggtactgt ggataatgtc tgttttataa
     1381 ttcggggtcc acattctgct tgggcagatc ttttatgggc acaccgttgg tctctgtttt
     1441 ataccagtgc ttatataaat aataaagagg tttatgattt tacttttcaa ccggaagacc
     1501 aaaatgatgt aactactctt tgtcatgaaa tgtttcatag tgttggtgct ccagatttat
     1561 atcattatga atatgacggt ttaactccgg ttggttgttg ggacttaatg gaaagtggag
     1621 atgggcatat ggggatgtat atgaaatatg cctatggggg atggatacct tctattcctc
     1681 ttgctcaaat tggtaatacc tatacactaa atccagttac ttctgctgaa aataatgtat
     1741 ataaataccc tatacctggt tcaaccaatc agtttttaat ttttgaatac cggaaaaaaa
     1801 gtagcgatat ttttgaagag aacttaccca attctgggct tttgatttat cgaattgatc
     1861 aaacaatgga tggtaatgta gagggtcctc cggatggagt gtatgtttat agacctgatg
     1921 gatctttaac caataatggc ttggttgctg aagccccatt cagtgctaat aattttagaa
     1981 ctgcttttaa tgaatacacg aatccatctt cttttctaaa taacggttct acttttccgg
     2041 ttaatattac taatattact gaagcaggtg aaactataag ttttacaata ttctctccag
     2101 cagtaactat ggctcctgtt attgacgcta tccatcctgc caatggttct attttagctg
     2161 ataatacgct gaatatttat cttcaagtta cagatcctgc ggaggctata cagcgtgtgg
     2221 aatatacttt ggatggcatt ttagtttata cagcaactgc tgaaccatat acaggtataa
     2281 taagcgatta tctattaacc cccggagtcc ataatattgg aataactgct ttttctgctt
     2341 ccaatttgtc tacaacagtt aatattaatt atcggatagt taatcccgat ctggaaaatt
     2401 ggttttcctg gctttcttct gagccacatt ggatagaata tggacgggga gctatcccaa
     2461 taaaagctgc tatagatatg gatttgggga atcaagaata tattgtaaaa gcgcttaggt
     2521 ttaatatcac acccgatcct tggggtgaac ctgatatgcc tggaatggta tatgcccgca
     2581 ttaatagatt ttccaatgga gcaattactg atcaacttct attagatatt ggctattgct
     2641 ataattttga ttatgatcct aattttactt ttaatgtggt ggatacgacc cacatatcag
     2701 gacagattgc tgtaatttta gacctctatg aatatcaaaa tatgcgcttt gaccaaaatg
     2761 ctgcttgtgg tcacagctgg atttatgaac ccaacagacc ttggacagat gctttgggaa
     2821 gaggaattgt tggcgctgct gccattgaac tacttttaca aaatcctaat gtgagtatcg
     2881 aagaacctaa ttgcccagcc ctgaatatat ccttatcaag ctatcccaat ccttttactt
     2941 ctacaacaaa aattaagtac tcgctacctg caagtggaaa agtgaattta actatctaca
     3001 acctcaaagg tcagaaagta aaaaacctgc ttacggaaaa taaaacagtt ggaaattatg
     3061 aaatggaatg gaacggaact gatacacaag ggaaaaatgt tggtagcgga ttatatcttt
     3121 gccgtttagt atccggaaat aggacggcta ctacaaagct tttaagatta tcagattaaa
     3181 gaaataaatt tcccatagtg aggagttctc tgcgcctata aaaaagttct cgtaaagaac
     3241 aaaattataa gggcatagag gattcctctc ttaactattt ctcaatataa taattcaagc
     3301 tctaaaattc cccaaaatcc tgttcatctt atgcctatta tcttttttat cgaggggctt
     3361 acgcaccatc gctaacactg tgttgacctt tgagttaaaa tcggttttgt ttgtggtttt
     3421 ccacacaatt tcattctttt tccctatctt tgaaaaaggg cactattaat ctcatagaaa
     3481 aggccgattt tgacttaaag tcgactaatt gtcagttata aaatataatc ttctccaatc
     3541 cttaaatcct tgtttctaaa aatgaatctg cgtgagttat tttcggtggt atcggtggct
     3601 aaaaactctt ctaacatcct tgtttataaa gctatatcct ctcaatccta aaatccttaa
     3661 atccttgttt cagaaaatct aatccttaca tccttgtttc taaaaatgaa cctgtgtgag
     3721 ttattttcgg tggtttcggt ggctaaatac tcatctaaca tccttgttta taaagctata
     3781 tcctcttaat cctaaaatcc ttaaatcctt gtttcagaaa atctaatcct tacatccttg
     3841 tttcgaaaat accatcagtg cataaggcct ttttcttaca ttatcagtgt gaatcagttt
     3901 aatgccataa atatctgtgc tattctatgc aaatccaaaa accaattgac ataaaccata
     3961 actttatttt tgaagtataa actaaagcaa atgtctataa aacaaggagc gaaaatgccc
     4021 tatatctcta ataccgataa tgataggcga aaaattctaa aggtaatcgg cgctaataag
     4081 tttgaggatt taatctctgc tattcctgcc aaactgagaa tgtgtaatcc tcttgctata
     4141 tccgaacccc tatcggaact ggaaataaac cgtaatatcc aaacaaaggc agataaaaat
     4201 aaaccttgta ttaaagtaaa ttcttttttg ggcgggggtg tttacgatca ttttattcct
     4261 gctgcagtgg atgccatagt ttcgcgtccg gaatttttta ctgcctacac tccttaccag
     4321 gcagaagtaa gtcaaggtac acttcagtac atatttgaat accaaacaat gatatgtgag
     4381 cttactggta tggagattgc caatgctgga atgtatgatg cagcaagttc aattgcggaa
     4441 gctattttga tggctgtgcg taaaaaccat ttaactaaag caattctgcc agcaacttta
     4501 cacccgaatt atgttaaagt gattaaagct tatactgaag gtatcggtat agagctttta
     4561 actgtgcccg aaaaagaggg agtgacagat ttatctgccc tggaagcaat gatggatgaa
     4621 acaattggaa gtgtagttct ccagactccc aatttttatg gcaacttgga agatgccaaa
     4681 gctatagata ctattgttca taagaactcc aagtgcttac taattgccag tgttgatcct
     4741 gtttctttag ctgttcttat acccccttca gaatataatg ctgatatagc agttggtgaa
     4801 ggtcaggcac ttggtaataa tatgtatatg ggaggtccgc tttttggatt ctttgctacc
     4861 aaattggata tggcgcgttt gatgcctggt agaattgtag gtggaacaat agacaaagca
     4921 ggtgaaaaag cttatgtttt aactttacaa gccagggaac aacatatcag acgagcaaaa
     4981 gcaacttcca atatatgttc caatgaatca ctttgcactt tggctgccgt agtatatttg
     5041 tgtttattgg gtaaagaagg gcttattgaa gtagctactc aatctgttca aaaagcacat
     5101 tatgcggcaa gcgaactttg taaaataccg agtttggctc ttgcttatcc caaagcaact
     5161 ttctttaaag aatttgtaat ccgcactccg aaaaaagccg cagatattat aaacgcttta
     5221 ctacctgaaa atatatatgc cggaattgat cttgctcctt acggaaaacc gaatgagctg
     5281 atgatagcga taaccgaaaa gaagagcaaa acagaaattg acagttttgt tcaggcgatg
     5341 aaggaggtag taaaatgagt aaaacaattt ttgaacatag ctctccaagt agaaaaggag
     5401 taactttacc caaaagagaa atagatacac ctttgccaac attgattcct gaaacaatgc
     5461 ttagaactaa gaaagtgcgt cttccggaag tgagtgaatt ggatgttatg cggcattata
     5521 tatctctttc gcataaaaac cactttatcg aaaagggatt atatccatta ggtagctgta
     5581 caatgaaata taatcccaaa ctaaatgaaa ccctggtaag gcattcatct tttactacct
     5641 tgcatccttt tcaaccagaa gaaacaatgc aaggttcttt ggaactgatg tatgaattgc
     5701 aaaatgactt agccgaaata tctggaatga aaggagttac acttcaaccc tcagcaggtg
     5761 ctcaaggtga atttactggt attaaaatta ttgctgctta tcataaagca aagggtaata
     5821 atcataaaga tacaatcatt attcccgata gcgcacatgg aactaatccc gcatcctgta
     5881 gtttggtcgg ttataaaaca gtggaactcg cttccaataa agaaggacgc tgtgacatag
     5941 ctaaacttcg cgaattagta aatgaaaata ctgcaggaat tatgattact aatcccagta
     6001 ccttagggct ttttgaaacg caaattgaag aaattgctga aattattcat agcgtagatg
     6061 ccctaatgta tatggatgga gccaatttga atgctctttt gggaatagta caacctggaa
     6121 aaatcggttt tgacttgatg cattttaact tgcataaaac ctttgctact ccacatggag
     6181 gaggtggccc aggttcaggt ccggtagctg taaacgaaaa attgctacct tttttgccgg
     6241 ttcctatagt tacgaaagac gaatgtggct atcatctttc ttatgaaaat gaagctactt
     6301 ccataggtaa agtgcatagt ttttatggta actttgctgt cctaatccgt gcctatatct
     6361 atattaagat gttaggagcg gaaggattgc gaagagtttc tgagaatgca attttaaatg
     6421 ctaattacct tatgaaaatt ctgggagatt attatcacat cgaacatcag gaaaactgta
     6481 tgcacgaatt tgtgatggat ggtaccaggc aaaagaagga atatggaatc actactttag
     6541 atattgctaa acggctttta gataaaggat tccattcccc cactatctat tttccactta
     6601 ttattcctga agcaatgatg gtggaaccaa ctgaaacgga atgcttggaa agtttggatg
     6661 cctttgccaa agcgatgatc gaaatcgctg aagaatgtaa agagaatcct gaacttgtta
     6721 aagaagcacc attaactacg ccagttaaaa gagtggatga tgtgcgtgct gtaaaagtgc
     6781 tggaaccaat ctttccgctt cccaattaat taaagtccgt ttatcaataa ggagaaaaaa
     6841 tgaaaaaaca tatcctagtc ctaattgttc tggtggcatc gtttgccttg gcttatgcac
     6901 aacctgtaaa acttggtttt gtaaatacag accgtctgct cctggaaagt aatgaagcgg
     6961 cagaagtagc caggttgttt gctttggata aacaaaactg gacaaatcaa attcgtactt
     7021 tggatgacga gattaagcaa atggaacgcg attttgaaat ccgtaagcta actatgaacg
     7081 atgctgctaa gagagaaact caaagcagaa tcgatactaa aaaaacagag gcaggtcgct
     7141 tgctggaaga atattttggc gatggaggaa aagcggaaca gcgttatcgt gaacttattg
     7201 atcccctaac tctgaaaatt cataacctga tcaagaaaat tgctgaagac gaaaaataca
     7261 ctatgatctt tgatgttagt atgggtagct tactttatgc acttcccact ttagatttaa
     7321 ccgagcaaat attgttggaa ctgaataaag ataccgtaaa accaacagat gtatcaccaa
     7381 cggatattaa ccctcccaaa gatcaaactc ctcccgaaat taaatcccct gagggttatg
     7441 aagaatttaa gccagataaa aaatgaaacg ctttagcaca aatatctcag aggaaatact
     7501 ccttcatatc tgccaagggg aacttatagg caaaatacct gaaaaccttt gtaaagttag
     7561 tgaaccacaa gaagcagatc aacaaaccat aatctttctg gaacaggaaa agctattaga
     7621 catcgttaaa gccagctctg caggcttaat tattactact aaaagatatg tacctatgtt
     7681 tccggataga gctttgctga ttgtggaaaa accctatttt gccttaatgc tactaattac
     7741 atatctactt aatcaagaaa acaagaatat cacctatact attcatccct ctgctgttgt
     7801 tgctgaagat gttattttag ataaagaagt ggccattgga gctaatgttg ttattggtag
     7861 tggtagcaaa ttaggcaaag gagttattat tggtgagggt tgctcaattg gcaaaaatgt
     7921 gtacatagga gaaagaacga aactttatcc taatgtttgc ctttatgatg attgcgtaat
     7981 tggtaataac tgtattttac acagtggcgt aattattgga gctgatggtt ttggctttat
     8041 gttaattgct ggcgttcaac aaaaaattcc ccaggtggga aatgttgtta ttggcaacga
     8101 tgttgaaatc ggagccaata gttgcataga tagggctact ttaggttcta ccataatagg
     8161 aaataacaca aaaatagata atctggttca aataggccat aattgcgttg ttggtgaaca
     8221 tagtgttatt tgttcccaag ttggtttagc gggaagcacg attgtaggag attttgttta
     8281 tctggcaggt caagttgggg tggcagatca ccttaaaatt ggcaatagag caatgattgg
     8341 agctcaatct ggagtatcca gcaatattcc tgatgatggg aaatattttg gctatccagc
     8401 tttggacgcg aatcttacta aacgcattat ggctgttcaa aaaaatctac cagaaatgta
     8461 ttttgcctac ctgaaagcca agaaaaaaga aacaaatagc ggagataaat aatggaatac
     8521 aaacaaacaa taggcggcga atcttcttat actggtatcg gacttcatac tggggaaatt
     8581 agcacgatta gatttaaacc tgcaggaccg gaagaaggga ttgtttttat cagaacggac
     8641 ttaccaggaa aacctgaaat tcctgctgat attgatcatg tagtagatat ttccaggggg
     8701 acaactattg gtattaatgg agccacagtt gccacaattg aacatgttct ttcagccgtt
     8761 gtaggtatgc gaattgataa tatccgcatt gagataaatg gaccagaagc tcctgtagct
     8821 gatggttctg ctgttgtttt cttaaatata ttaaggcaat gtggtattat gcaacaggat
     8881 agtgaacggg a
//
LOCUS       DAORHB010000038         8719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_7540_length_8719_cov_4.007618, whole genome shotgun sequence.
ACCESSION   DAORHB010000038 DAORHB010000000
VERSION     DAORHB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7540_length_8719_cov_4.007618"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PKM80_02100"
     CDS             complement(<1..148)
                     /locus_tag="PKM80_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNV92728.1"
                     /translation="MKFFIETYGCQMNIADSELIASILIEAGHQEVEQIEKADLLIFN
                     TCSVR"
     gene            complement(145..2145)
                     /locus_tag="PKM80_02105"
     CDS             complement(145..2145)
                     /locus_tag="PKM80_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92729.1"
                     /translation="MLRFALYVSNHGFGHSTRISALAEEFIKYGIFCHIISTKPAYLF
                     QNLDPHYFIIHQRSVDVGVKHNENLTVDMERTVSSLLELLSKRNEIIAREVKFLHQEK
                     IDCVIADVPFLVSEFGAYCQLPVYAVTNFEWHYIYSSLLTNNNIINSQTEFTNPVTLR
                     PVLNLIWSLYQRFDACFQLPFSSSESISAFKNNIPCGLLSRKKDSYSDIRLINNWDKD
                     VLILLVIFGGEGKLELDYEALCSAFKGVVISTQTGVQAKNHYQVTMDADFLDLIYNAD
                     FVLCKPGYSTLAEAVQMNKFIIYCPRKKYPEETELIQGLSKYSNCLQIDTLQMNTKQW
                     KAIFESIHIRKQPVDQYKNNNINVAGSILKKYAQMIGNNLVSVFDLGSNNLNYLLYSL
                     DNKQIIHKTQLTTSLGKDFKNDKLSAKRLQSVKQIIEPLLQIDGYIESKKVLLATGVM
                     RKALNANLLTDWIEKHYEIKSKIISQEEEIRYVYYAARHFQSGEEAYLAIDIGGFSTE
                     FIDLGNDEKIQGIGLPFGLMTLLNEFDVNCDTATKYIQDQLATLTYNGSYHLIGVGLT
                     YTYLAAVIFKFHYNDIDKMEGKTITRSLLQQLFHSIEVKDEDRYLPFLLEESYLPVLK
                     LSILFIISLLDRFNTSEIIVCNSGIAVGYANWLAKTKNKALK"
     gene            complement(2153..2521)
                     /locus_tag="PKM80_02110"
     CDS             complement(2153..2521)
                     /locus_tag="PKM80_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNV92730.1"
                     /translation="MKKDSILNDLRPADLVLILIVLLTIVFSISYYLSSKPNSQVYIY
                     KDNWLVGVCSLKNDKIIKIDSHNIVEIKKGKVRMKYADCPDKRCVKQGFTNSMPIICL
                     PNKVIIEIRNNGEQKKLILH"
     gene            complement(2522..3385)
                     /gene="amrS"
                     /locus_tag="PKM80_02115"
     CDS             complement(2522..3385)
                     /gene="amrS"
                     /locus_tag="PKM80_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNV92731.1"
                     /translation="MRKAMYYTNEGEKIKCLLCPRECLLSEGQKGFCRSREVINGSFV
                     ATAYGKTTSLALDPIEKKPLYHFHPGSMIVSLGPNSCNLTCKFCQNWEISQQDCPTRY
                     LAIEDLIEIIKNQQDQKVAFTYTEPLMWYEYILDFAAKAPEIDIVLVTNAYINKEPWS
                     NMMQYVKAVNIDIKSFRDDFYRNICGGTLEVVLSNIIIAKEMGVHLELTNLLIPGYNN
                     SDEELTNLSQFIASVDKNIPLHLSAYYPCYKMTVRPTTREEVEHACEIASKYLTYVYA
                     GNVYSSRYSRS"
     gene            complement(3385..4620)
                     /locus_tag="PKM80_02120"
     CDS             complement(3385..4620)
                     /locus_tag="PKM80_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNV92732.1"
                     /translation="MILLSIFNIILEVVYFLFFPCIYLLLKHKNYVEAIKANGINIQK
                     GILFHSASMGELTAIKSLIKKHLQEYPDIKLCITSSTVSSCLEANNISPKVKGFLSVI
                     DIPHLRNRQLKKINPGLICVVETEIWPNMLLWAKRNKVPVLFLNARMSKSTLRGYSLI
                     KFLFKHLQAPITEIHTQSEEDAKRFREIFKKPVLVSGNLKFSIELKDYDAEQLKKEWG
                     YNNDDFIICWGSSRPGEEELLISILPYLKQYISNLHLIIAIRHPRRLDEVLELLNGLA
                     YSCYTKREIYDPAEDILIIDTLGILDKAYCICDIAIVGGSFYDFGGHNPLEPAYYKKP
                     VIIGPYHYSCLDSVKKLQQEGGILISDKNSLHSDIITLYKDNELRYNMGEKAKKVLTQ
                     NARTLDIYLKALEKWLKEI"
     gene            complement(4667..4744)
                     /locus_tag="PKM80_02125"
     tRNA            complement(4667..4744)
                     /locus_tag="PKM80_02125"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4707..4709),aa:Asp,seq:gtc)
     gene            complement(4876..6411)
                     /gene="guaA"
                     /locus_tag="PKM80_02130"
     CDS             complement(4876..6411)
                     /gene="guaA"
                     /locus_tag="PKM80_02130"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNV92733.1"
                     /translation="MHNKVLILDFGSQYTQLIARKIRSLNIYCEIHPFTISADKLYSF
                     APKAIILSGSPYSINNNETPKPDECIWQLGIPILGICYGMQLMVAHFGGKVQPSAKRE
                     YGLAYIKIIDHNELLADLGNREEVWMSHSDAISNLPECFSVLAETENSPYAAIAHNEL
                     PFYALQFHPEVVHTKNGIKILENFLYRIVNLTPDWTAGTFINKSIEKIRSQVGDKKVI
                     LGLSGGVDSTVTAALLYRAIGKQLLPIFVDTGLMRYREADRVKETFSAYPDLNIRFVD
                     AGETFLNKLKGITDPETKRKIIGGTFIEVFDKVASEFEDVHFLAQGTLYPDVIESASL
                     TGPSATIKSHHNVGGLPDNMLLVLIEPLRELFKDEVREVGTLLGLPESIVQRHPFPGP
                     GLAVRILGEVDAQKVNILQLADEIFISELQENGLYNKTWQAFVVLLPVRTVGVMGDER
                     SYEQVCALRAVDSVDGMTATFSQLPFHFLQKVANRICNEVKGISRVVYDLSSKPPATI
                     EWE"
     gene            complement(6420..7046)
                     /locus_tag="PKM80_02135"
     CDS             complement(6420..7046)
                     /locus_tag="PKM80_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV92734.1"
                     /translation="MLKIESTVLLESYQTNTYLLWETDSSNAFLIDPANPSEAFLKRI
                     RQLELKVKMIINTHGHADHIGGNHFFAKALNCPVAIHSLDAEMLTDNSKNFSIYMGFE
                     LSLFGPQVILNDGDELFLGTDKVKVIHTPGHTKGSICLLADKYLISGDTLFEQSIGRT
                     DFPGGSHTAIIDSIKNKLFILDDEVLVFPGHGFTTSIGLEKKNNPFIY"
     gene            complement(7063..8055)
                     /locus_tag="PKM80_02140"
     CDS             complement(7063..8055)
                     /locus_tag="PKM80_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HNV92735.1"
                     /translation="MKIHKAIALFSGGLDSIIAVKWMQNRGYSVHSVFFKAPYIFPER
                     ALHYASKNNIELEVIDISEDHIKLLDNPVYGFGKYFNPCIDCHGLMFKKAAELLPSRN
                     ADYIISGEVLGQRPKSQRHDALDSVGRLSGVKDLIIRPLSQNLLSDTLPLREGWIDKQ
                     DMLSIHGRGRYAQLALAEQLGIVDFPSPAGGCLLTDRNYCLRIKDLKKHQQDTLENIE
                     LLAWGRHFRLDNETKLIIGRDEAENEVLENMFRNGTKLQARDYMGPVGFITGNASYET
                     LSLALSIFLLYHPKAPENAAIEIWKVIDKQLTQHTVKIATKAKKELNKDYLISY"
     gene            8383..>8719
                     /locus_tag="PKM80_02145"
     CDS             8383..>8719
                     /locus_tag="PKM80_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92736.1"
                     /translation="MKNIALLVLLVFSTAVLIAGVSVNYSLEMPKIDSNGQYTKIKLE
                     NTQSWGQPGNPDLPWFGIKILLPEGMEADKIVVSKTDPVVYSLPKRVSPIQKQYPFSH
                     KIIETPDAPN"
BASE COUNT         2847 a         1625 c         1370 g         2877 t
ORIGIN      
        1 cgcgaacact gcatgtattg aatataagta aatcagcttt ttctatttgt tcaacttcct
       61 gatgaccggc ttcaattaaa atggaagcga ttaattcact atcagcaata ttcatctgac
      121 aaccgtatgt ttcaataaaa aatttcattt tagcgcctta ttttttgttt tagcaagcca
      181 attagcgtag ccaactgcta ttcctgaatt gcatacaatt atttcggagg tattaaatct
      241 gtccagtaaa gaaataatga aaagtatact taacttcagc acaggaagat agctttcttc
      301 cagcaaaaat ggtaaatatc ggtcttcatc tttaacttct attgaatgaa aaagttgctg
      361 caataaagac ctagtaattg ttttaccttc cattttatct atatcattat agtgaaattt
      421 aaaaatcact gctgctagat aggtataagt tagtcctact cctataaggt gataagaacc
      481 attataggtt aaggtagcaa gctgatcttg aatatatttt gtagccgtat cacaattgac
      541 atcaaattcg tttaacaatg tcattaaacc aaaaggtaga ccgataccct gtattttttc
      601 atcatttcct agatcgataa actccgtaga aaagccacct atatcgattg caagatatgc
      661 ctcttcccca ctctgaaaat gtctggctgc ataatataca tagcgtattt cttcttcctg
      721 ggagataatt ttacttttta tttcataatg cttctcaatc caatcagtta gcaaattcgc
      781 attcaatgcc tttcgcataa ctcctgttgc taaaagcact tttttggatt cgatatatcc
      841 atcaatttgc aataatggtt ctataatctg ttttacgctc tgcagccgct ttgcactaag
      901 cttatcattt ttaaaatctt tccccaggga tgtcgtcaat tgcgttttgt ggataatttg
      961 cttattatca agggaatata gtaaataatt caaattattg gaaccgagat caaacacact
     1021 gacaaggtta ttgcctatca tttgtgcata tttttttagt attgatccag ctacattgat
     1081 attgttattt ttatattgat caacaggttg tttccgaata tgaatgcttt cgaaaattgc
     1141 tttccactgc ttagtattca tttgcaatgt atctatctgc aaacagttgg aatatttact
     1201 taaaccttgt attaactctg tttcttcagg atacttctta cgggggcagt agatgataaa
     1261 tttgttcatt tgcacagctt ctgctaaggt actataacca ggtttgcaaa gaacaaaatc
     1321 cgcattgtat attaaatcca aaaagtcagc atccatagtt acctgataat ggttttttgc
     1381 ctgtacacct gtctgagtag aaatgaccac acctttgaaa gcagaacaca gagcttcata
     1441 atccaattct aatttacctt cccctccaaa gataaccagt aatataagta catccttatc
     1501 ccaattattt ataagccgga tatcactata agaatctttt ttacgggaaa gcaaaccaca
     1561 aggaatgtta ttcttaaaag cagatatact ttcagaactg ctgaaaggaa gctgaaaaca
     1621 agcatcaaag cgttgataca aagaccaaat aagatttaat acaggtctca aagttacagg
     1681 gttagtaaat tcagtctggc tgtttattat gttgttatta gttagtaaag agctatatat
     1741 ataatgccat tcaaaattgg taactgcata aaccgggagc tgacaatatg ctccaaactc
     1801 actaaccaaa aaagggacat cagcaattac acaatcaatc ttttcttgat gtaagaattt
     1861 aacttctctt gctataattt cgttacgctt agaaagtaat tccaataaag aagaaacggt
     1921 cctttccata tccacagtta gattttcatt atgtttcact ccaacatcca cagaacgttg
     1981 atgtataata aaataatgtg gatcgagatt ttgaaaaaga taggcaggct ttgtgctaat
     2041 tatatgacaa aaaataccat actttataaa ttcttcagct aaagcgctta ttcgggtaga
     2101 atggccaaaa ccatgattag aaacatataa agcaaatcgc aacatattat atttaatgaa
     2161 gaatcaactt tttttgttca ccgttattgc gtatttctat tatcacttta ttgggtaaac
     2221 aaataatcgg catagaatta gtgaaacctt gcttaacgca acgtttatca ggacaatcgg
     2281 catatttcat tctaacttta ccttttttta tttccacgat attgtggcta tcaatcttga
     2341 ttattttatc attctttaaa gaacatactc caacaagcca attatccttg tatatatata
     2401 cttgggaatt gggtttacta cttaaataat agctgatcga aaaaacaatc gtcaataaaa
     2461 caattagaat tagtaccaga tcagcaggcc taaggtcatt taagattgag tcttttttca
     2521 tctaacttct ggaatatcga gaagaataaa cattacctgc atacacataa gttaaatatt
     2581 tagacgcaat ctcgcaagcg tgttcaactt cttctcttgt ggtaggtctc accgtcattt
     2641 tataacaagg ataatatgca gagaggtgta aaggaatatt cttgtcaaca gaagcgataa
     2701 attgagataa attagtaagt tcttcgtcac tattattata tccaggaata agcagattgg
     2761 ttagttctaa atgaacaccc atttctttgg ctataatgat gttagataat acaacctcaa
     2821 gtgtacctcc acatatattt ctataaaaat catctctaaa agactttata tcgatattga
     2881 ctgcttttac atattgcatc atattactcc agggctcttt atttatgtag gcattggtta
     2941 ctaaaacaat atctatttca ggagcttttg ccgcaaaatc gagaatatac tcataccaca
     3001 tcaagggctc agtataagta aaagcaactt tctgatcttg ttggttttta attatctcaa
     3061 ttaagtcttc aattgccaaa taacgagttg ggcaatcttg ttgactgatt tcccaattct
     3121 gacagaattt acaggttaaa ttacaggagt ttggtcctaa agatacgatc atactacctg
     3181 gatgaaaatg ataaagaggt tttttttcga tagggtctaa agcgagagag gttgtttttc
     3241 catatgcagt ggcaacaaaa gaaccattga taacttctct gcttctacag aatccttttt
     3301 ggccttcact taataaacac tccctggggc aaagcagaca ttttattttt tcgccttcat
     3361 tagtataata cattgctttt ctcattatat ctccttcagc catttttcta aggccttaag
     3421 gtaaatatct aaagtgcgag cattttgagt caagaccttt tttgcttttt cccccatatt
     3481 ataccgtaac tcattatctt tatacaaagt tataatatca gaatgtaagc tatttttatc
     3541 gcttattaaa attccaccct cctgctgaag ttttttaacg gaatcaaggc aggaataatg
     3601 atagggacca ataattactg gttttttata gtaagcaggt tcaagtggat tatggcctcc
     3661 aaaatcataa aaagaaccac ccacaatagc tatatcgcat atacaatatg ctttatccaa
     3721 aatgcccaag gtatcgatga taagtatatc ttctgctgga tcgtaaattt ctcttttagt
     3781 atagcaacta tatgctaagc cattcaatag ttccaaaact tcatctaaac gtctgggatg
     3841 tcggatagcg attattaagt gtaaattaga gatatattgt tttagataag gcaagataga
     3901 aataagcaat tcttcttctc cgggacggga agagccccaa cagataataa aatcgtcatt
     3961 attatatccc cattcttttt ttaactgttc ggcatcatag tccttcaatt cgatagagaa
     4021 tttaaggtta ccactaacaa gtacaggttt tttgaatatt tcacgaaagc gttttgcatc
     4081 ttcttcgctc tgggtatgaa tttcagttat tggtgcttgc agatgtttaa acaaaaactt
     4141 aatcaaggag tatccacgta aggtgctttt ggacattctg gcatttaaaa acaaaacagg
     4201 aactttattt ctctttgccc atagcaacat atttggccaa atctctgttt ccacaacaca
     4261 gattaagcca gggttgattt tttttaactg acggtttcgt agatgcggta tatcgataac
     4321 tgaaagaaaa cctttaactt tggggctaat gttattagct tcaaggcaac tggatacagt
     4381 gcttgaagtt atacaaagtt taatatcagg atattcctgc aggtgttttt ttataagtga
     4441 ttttatggca gttaattcac ccatagaagc agaatgaaac aagatccctt tttgtatgtt
     4501 tataccgttg gctttaatag cttccacata atttttatgt tttaatagaa gatagataca
     4561 cgggaaaaaa aggaagtaaa caacctcgag gataatatta aaaattgaaa gtaaaatcat
     4621 aatttatatg ttatagcaaa ttaagatttg ccatcctaat ttaaaatggc gggagcgacg
     4681 agactcgaac tcgcggcctt ctgcgtgaca ggcagacgtt ctaaccaaac tgaactacgc
     4741 ccccagcgca ttcaaataag cattataaaa attcaaatgg aaaaaatggc tttttctgtc
     4801 aagataaaag ttttgctgaa ttcgccactt tatacataaa cgcatagcat tcattgcagt
     4861 tagcataaga atattttatt cccattcaat tgtagcaggt ggtttggaag ataaatcata
     4921 aactactcta ctgatacctt ttacttcgtt acaaatacga ttggcaactt tttgcaaaaa
     4981 atgaaatgga agctgagaga aagtagctgt catgccatca acactatcca cagctctaag
     5041 agcacaaact tgttcatagc ttctttcatc tcccataact ccaacagttc gaactggtaa
     5101 taaaactaca aatgcctgcc aggttttatt ataaagtcca ttttcttgta attcacttat
     5161 aaagatttca tcagcaagct gcaagatgtt aactttttgg gcatctactt ctcccaaaat
     5221 tcttacggcc aaaccaggac ctggaaacgg atgtctttga acaatagatt caggtaaacc
     5281 aagcagggtc cctacttcgc gaacttcatc cttgaaaagt tctcttaagg gttcaattaa
     5341 taccaagagc atattatcag gcaagccccc tacattatga tgacttttga ttgtagcaga
     5401 aggtcctgtc aaagatgcac tttcaattac atcgggatat aaggtccctt gagcaagaaa
     5461 atgaacatct tcaaattcac tggcaacttt atcaaagact tcaataaaag ttcctccgat
     5521 tatttttcgt tttgtttcag gatccgtaat accttttaat ttatttaaaa atgtttcccc
     5581 cgcatctaca aaacggatat ttaaatctgg ataagctgaa aaggtttctt ttactctatc
     5641 tgcttccctg taacgcatta aacctgtatc tacgaaaatg ggtaatagtt gttttccgat
     5701 tgctctataa agtaaagcag cagtaaccgt agaatcaacc ccaccactta agcccaaaat
     5761 aactttttta tcaccaacct ggctcctgat tttttcaatg cttttattaa taaaagttcc
     5821 agctgtccaa tcaggagtta gatttactat cctatagaga aaattttcta aaatcttgat
     5881 cccattttta gtatgtacta cttccggatg gaattgtaat gcataaaaag gtaattcatt
     5941 atgagcaata gcggcatagg gagagttctc tgtttcagct aaaacagaaa agcattcagg
     6001 caaattggaa atagcatcac tatgactcat ccagacctct tccctatttc ctaaatcagc
     6061 aagcaattca ttatggtcaa taattttaat atatgctaaa ccatattctc ttttagcaga
     6121 aggttgaact tttcctccaa agtgtgcgac catcaattgc ataccataac aaattcccaa
     6181 aatgggtatt ccaagttgcc atatacattc atctggttta ggagtttcgt tattatttat
     6241 gctatatgga cttccggata atatgatcgc tttaggggca aaggaatata gtttatctgc
     6301 acttattgtg aaaggatgaa tctcacagta gatgttcagg gaacgtattt ttctggctat
     6361 caattgggtg tattgagaac cgaaatctaa tattaaaact ttattgtgca ttgctactcc
     6421 taataaataa aaggattatt tttcttttcc aaaccaatgg aagttgtaaa cccatgacca
     6481 ggaaaaacta aaacttcgtc atctaatata aagagtttat tctttataga atcaattatg
     6541 gctgtatgac ttccaccagg aaaatcggtc ctaccaatgc tttgttcaaa taaagtatct
     6601 ccactgatta gatatttatc tgctaaaaga catatactac ctttagtatg acccggagta
     6661 tgaataactt tcactttatc cgttccaaga aacagttcat cgccatcatt caaaataact
     6721 tgtgggccaa ataaacttaa ttcaaaaccc atataaatac taaaattttt gctattatct
     6781 gtaagcattt ccgcatccaa actatgaatg gcaacaggac aatttagagc tttcgcaaaa
     6841 aagtggtttc ccccaatatg atcagcatgg ccatgagtat ttataatcat tttaactttc
     6901 agctcgagtt gtctaatacg ctttaaaaat gcttcactgg gattggcagg atcgattaaa
     6961 aatgcatttg aactgtcagt ttcccaaagc agataagtgt tggtttgata actttccagt
     7021 agaacagtac tttcaatttt taacatcttc tgacctcttt tattaatatg atatgaggta
     7081 atctttattt aactctttct tagcttttgt ggcaattttt acagtatgct gagtaagctg
     7141 cttatctata actttccaaa tctcaatagc agcattttct ggtgccttag gatgatataa
     7201 caaaaatata ctcaaggcta aacttaatgt ttcatagcta gcatttccag ttataaatcc
     7261 tacaggcccc atataatctc gtgcctgcag ttttgttcca tttctaaaca tgttttctaa
     7321 tacctcattt tctgcttcat ctctaccaat aatcagcttt gtttcattat ctaaacgaaa
     7381 gtgacgaccc caggcaagaa gttcaatatt ctccaaggta tcctgttgat gtttcttcag
     7441 gtcttttatg cgtaaacaat aattacggtc tgttaataaa catcctcccg caggagaagg
     7501 aaaatcaact attcctaatt gttcagcgag tgccaattga gcgtatcttc cgcgtccatg
     7561 aatagatagc atatcttgtt tatcaatcca accttctctg agaggtaagg tatcagaaag
     7621 taaattttga cttaatggcc taataattaa atctttaacg ccgcttaatc tgccaacact
     7681 atctaaggca tcatgcctct gacttttggg tcgttgacct aaaacttcac cactaatgat
     7741 gtaatctgca ttgcgagaag gtaataattc tgcggctttt ttaaacatca atccatgaca
     7801 atcaatacaa ggattaaaat actttccaaa accatatacc ggattatcca acagcttaat
     7861 atgatcttct gaaatatcta taacttccaa ttctatgtta tttttggaag cataatgaag
     7921 cgctcgctcc ggaaaaatat atggagcttt aaaaaaaacc gaatgcacag aatatcctct
     7981 attttgcatc catttaacgg ctatgatact atctaagcca ccagaaaaaa gggctatcgc
     8041 tttatgtatt ttcatattac ctacccctga aaaaagcact tttttgtcaa gcaggaattc
     8101 taggttgaag gttgatatcc ctccaccagt gattatacac tcttctgtga gatgcctttt
     8161 taatcttctt ccctttttct tgatttggga tccggtgtca acttttttag tatgattcaa
     8221 tttctcttag gcgattctct aacgagatta aagaatcgtt aatgatttgt tagaataata
     8281 ttagccaaat gatagagaaa tgtgtattat ttcccgagaa tggaacagac cttgcataat
     8341 atattaagaa gaaaaatcaa agaacaaaaa gggtagcata aaatgaaaaa catcgcatta
     8401 ttagtattac tagtgttttc tactgcagtt ctaatagctg gagtatcagt gaattattcc
     8461 ttagaaatgc ccaagattga ctcaaatgga caatacacaa aaataaagtt agaaaatact
     8521 caaagctggg gacaaccagg aaatcctgat ctgccctggt ttggaataaa aattttattg
     8581 cctgaaggta tggaagcaga taaaattgta gtttccaaaa ccgatccagt tgtatacagc
     8641 cttcccaaac gagtatcccc tattcagaaa caatatcctt tttctcataa gataattgaa
     8701 actccagatg caccaaatc
//
LOCUS       DAORHB010000039         8627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_7655_length_8627_cov_4.548413, whole genome shotgun sequence.
ACCESSION   DAORHB010000039 DAORHB010000000
VERSION     DAORHB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7655_length_8627_cov_4.548413"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PKM80_02150"
     CDS             complement(<1..522)
                     /locus_tag="PKM80_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92737.1"
                     /translation="MPKITIVTLLPGEIEKVIDVCKFSPINSELEIEIIYPQKDVSSH
                     QAGNLKLTKFEDVKELPVPDKSHICFFASPISCDLYRKQAKINVSSLYVHPELAILQL
                     QSDNPFKTKLSDYGLSPCVLEGDAAADLYIEQDRLIYFCDFQQFMQNKKPNFVLLSTN
                     TQFVESLISMDSAH"
     gene            complement(529..1326)
                     /gene="pyrE"
                     /locus_tag="PKM80_02155"
     CDS             complement(529..1326)
                     /gene="pyrE"
                     /locus_tag="PKM80_02155"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNV92738.1"
                     /translation="MEEKVIVTVATYTNLFEAELAKSLLEDSGFHPNLLNERMMGMYA
                     SIAGDMYKIELQVPESEEQEAKELITGLEDSDYVTRILQSEGALLDGHFQLTSGKHSN
                     QYIEKIRILQNPEAAHNLCKRLAEHLVEYEFDAVVGPAYGGIALAFEVALIMKKNFLF
                     TQRKDGEMTLRSGFDLSKLKKVAIVEDILTTGGSIQEVISCLENRGLEVVAVGVLVDR
                     SGGKVQFPVPLESLFCIDIPAFNPDNCDACKQGLPLVKPGSSDKVSF"
     gene            complement(1418..1963)
                     /locus_tag="PKM80_02160"
     CDS             complement(1418..1963)
                     /locus_tag="PKM80_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV92739.1"
                     /translation="MTTEMICAGFGGQGVLTIGKFIAQAAMQEGKNVSWLPSYGPEMR
                     GGTANVSTVVSTEPIASPIVSFPDVLVALNQPSIDKFAHSIRPGGVLIYNTNMCPRGC
                     QREDIIQIAVPMNEIASEIGSMIVLNMLATGIIIGKTNIIKYETLEGNLTSFLKEKNP
                     DLLAKNLAAIQRGIEIGKQYA"
     gene            complement(1963..2712)
                     /locus_tag="PKM80_02165"
     CDS             complement(1963..2712)
                     /locus_tag="PKM80_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV92740.1"
                     /translation="MEIIAKRPEALTKEPFHYCPGCMHGVAHRLIAEAIDENGLINEI
                     AGVAPVGCGVFAYKYFNFDMAEAAHGRAPAVATGIKRARPDMHVFTYQGDGDLAAIGT
                     AEIVHAANRGEKISVFFINNAIYGMTGGQMAPTTLPNMKTTTSPYGRNVEDIGYPIRV
                     CELLSTLVAPYYIERVSLLTSADIIKAKKAVEKAIRYNKENRGFTFVEFISTCPTNWG
                     YEPEKARQWAKENMLPFFKPGVFRDKGEGVE"
     gene            complement(2728..3807)
                     /locus_tag="PKM80_02170"
     CDS             complement(2728..3807)
                     /locus_tag="PKM80_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNV92741.1"
                     /translation="MSKILMKGNEAIAEAAIRSGCRLYFAYPITPQSELIEYMAKMMP
                     KVNGTFIQAESEVAAINMVYGAAGSGKRVMTSSSSPGISLKMEGISYIAGAELPCVIV
                     NVQRGGPGLGDIQPAQGDYFQATKGGGHGDYRLIVLAPSSVQEFADMASEAFDLADKY
                     RNPVMILADGMLGQMMEPVEFKPAKTDAELQELANQHLSWCICPNADGDKKHHHEINS
                     LELDPQVLEKHVLDLYKKYAEIEKNEARYETYNISDDNEVLCVAWGTASRVVKSAINE
                     VMKEGKSVGLIRPIRVWPYPYDAIKQAIGKNVKKVYVFELNSGQMVEDVKIAVEGKVP
                     VDFWGKLGGVVFTPAEIKEKLETCF"
     gene            complement(3816..4019)
                     /locus_tag="PKM80_02175"
     CDS             complement(3816..4019)
                     /locus_tag="PKM80_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNV92742.1"
                     /translation="MPKMTVDPDYCKGCGLCINACPRKIIRLSENINAKGYNYAECFE
                     QEKCTACKLCYITCPDVAITIEK"
     gene            5421..5915
                     /locus_tag="PKM80_02180"
     CDS             5421..5915
                     /locus_tag="PKM80_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNV92743.1"
                     /translation="MKKLKKKLTACFSAFLLLFIAPVIAQTVASVNEEEFFTDRSLTV
                     IDSLQTQEPPGAPPGEKMIATYYSKRFHGRKTASGERYNRDAFTCAHKSLPFQTLLKI
                     TNPQNGKSVTVRVNDRGPFNRNRDIDLSYAAAKEIDMLAAGVLPVEVEIMMSSYTDSL
                     LAQH"
     gene            5971..6888
                     /locus_tag="PKM80_02185"
     CDS             5971..6888
                     /locus_tag="PKM80_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNV92744.1"
                     /translation="MHSLLNYSSRIKKALDLGKPILAMESTVLTHGLPYPDNLDILET
                     LEELCYSLEVEPATIIVLDGQLHIGMDDQEKEILKNKLQHSQELNKFSMRDIPYAINY
                     KKSGGTTVSATMLLSYKAGIRVFATGGIGGVHKSWNETLDISSDLKALSEIPIIVVSA
                     GCKAILDIPATLEVLESEAVPVLGWKTNEFPAFYSQKSGKKIDKIESAKDVVNIYKTI
                     QKLYPNPTGILVANPVPEKDQIPYKEIAEHIIAAIDEAKAKGISGKELTPFLLANLAE
                     KTAGKSVQTNLALLKNNVLIGAQIAKELK"
     gene            6885..7715
                     /locus_tag="PKM80_02190"
     CDS             6885..7715
                     /locus_tag="PKM80_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNV92745.1"
                     /translation="MKIYISTDMEGMPGTFNWEQEKTNRPEVMKNYQAHISDCLNAIL
                     YSGFNEFIEEITVADSHSGGDNLSYDITSLDKRITLISGGPRPQYMMPAFSSEYSMVF
                     LLGYHSGTGALKGNMDHTYSNSKIFKIWINDIPMNEAIINSAYAGYLGVPVALISGDK
                     ALEEEITTAMPWVHFVRTKEAVAKFAAKNYSSIVVKEALVKAVQASLAKKDIPLYTFQ
                     SPIHLKIEFHSTSMTDCAALMPHTKRLDGRTIEFIDDDYGVIFEAIMALVTLAYGSIL
                     "
     gene            complement(7954..8568)
                     /locus_tag="PKM80_02195"
     CDS             complement(7954..8568)
                     /locus_tag="PKM80_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNV92746.1"
                     /translation="MHSDPKEKEQNGDGRMKKDIILAGVGGQGILTIATILGTAAIKR
                     GMNLKQAEVHGMSQRGGDVQSHLRLSDDPIWSDLIPYGSADMILSVEPLEALRYLPYL
                     KEDGWIISNSTPFKNIPNYPPEEEIYREIKKIPNYVLFDADTLANAQKANRSMNIVVL
                     GAAITHLGFSQEEIEEAIKAIFAPKGEAIVEANLRALLAGIETK"
BASE COUNT         2717 a         1686 c         1494 g         2730 t
ORIGIN      
        1 atgtgcacta tccatactta taagggattc cacaaattga gtattagtgc ttagcagaac
       61 aaaattcggc tttttattct gcataaactg ttggaaatca caaaaatata ttaatctatc
      121 ttgctcaata tataaatcag cagctgcatc tccctccaaa acacaagggg ataaaccata
      181 atcagaaagt tttgttttaa atggattatc gctttgaagt tgtaatattg ccagttctgg
      241 atgcacatag agagaagata catttatttt tgcctgttta cgatacagat cacaggaaat
      301 aggacttgca aaaaaacaaa tatgactttt atcgggaaca ggaagttcct taacatcctc
      361 gaattttgtt agttttaagt ttcctgcttg gtgggaagaa acatctttct gaggatagat
      421 aatctcaatt tccagctctg aatttatcgg agagaatttg caaacatcta taaccttttc
      481 aatttcgcca ggtagaagtg ttacaatcgt tatcttgggc atataagatt agaatgaaac
      541 tttatcgctg ctaccaggtt ttactaatgg taaaccttgt ttacaagcat cacaattatc
      601 aggattaaaa gctggaatat caatacaaaa caaagattcc agtgggacag gaaattgaac
      661 ttttccacca ctgcgatcaa ctaaaactcc tacagcaaca acttccagcc ccctattttc
      721 aagacaggat ataacttctt gaatacttcc gccggtagta agaatatctt ccacaatggc
      781 tacttttttc agctttgaga ggtcaaaacc gcttcgcaaa gtcatttcgc catcttttcg
      841 ctgggtgaat agaaaatttt tcttcattat taaggcaact tcaaaggcaa gagcaattcc
      901 tccataagca ggaccaacta cggcatcaaa ttcatattca acaagatgtt ctgcaagacg
      961 cttacaaaga ttatgagctg cttcaggatt ttgcagaatt cttatttttt cgatatactg
     1021 attgctatgt ttgcctgagg ttaattgaaa atggccatcc aaaagagctc cttcactttg
     1081 taggattctt gttacataat cagaatcctc cagaccagtt atcaattctt ttgcttcctg
     1141 ttcttcactt tccggaactt gtaattcaat tttgtacata tctccagcaa tagaagcata
     1201 catacccatc attcgttcat ttagtaaatt tggatgaaaa ccagaatcct ccaataagct
     1261 ctttgctaat tcagcttcaa agaggttagt gtaggtagcc acagtaacta taactttttc
     1321 ttccattgtc tcttccccct agttaactaa aatgtaaagt attccaggaa ttggaaagat
     1381 agagactctc caattcctgg tattattata gtgttattta agcgtattgt ttaccaatct
     1441 caataccccg ttgaatagct gcaagattct ttgcaagtaa atcgggattc ttttctttca
     1501 ggaaggaagt taaattacct tccagagttt cgtacttaat aatgttggtt ttaccaatta
     1561 taataccagt tgccaacata ttgagaacaa tcatactgcc gatttctgaa gctatttcat
     1621 tcatcggaac cgctatttga ataatatctt cgcgttgaca gccccgaggg cacatattag
     1681 tattataaat cagaactccc ccaggacgaa tagaatgggc aaatttatcg atcgacggtt
     1741 gattcaaggc aaccaataca tcaggaaaac taacaattgg cgaagcaatt ggttctgtgg
     1801 aaacaactgt tgaaacatta gcagttccac ctctcatttc aggtccgtaa gatggtaacc
     1861 aggaaacatt tttcccttct tgcatagctg cttgagcaat aaatttgcct atcgtaagaa
     1921 caccttgacc gccaaaaccg gcacatatca tttcagtagt cattattcca ctccttcacc
     1981 tttatcacga aaaacacctg gtttgaagaa aggcagcata ttttccttag cccattgcct
     2041 tgctttttca ggttcataac cccaattagt agggcaagta ctaataaatt ctacaaaggt
     2101 aaaaccacgg ttttccttat tataacgaat cgctttttct actgccttct ttgccttaat
     2161 aatatcagca gaggttaaaa gtgaaactct ttcaatgtaa taaggagcca ccagtgtaga
     2221 taatagctca caaaccctga ttggataacc aatatcttca acatttctac cataaggact
     2281 ggtagtggtt ttcatattag gtaaagttgt tggagccatt tgtccacctg tcattccata
     2341 gatagcatta ttgataaaga aaacgcttat cttttctcca cggtttgcag catgaacaat
     2401 ttctgcagtt ccaatagcag ctaaatcacc atctccctga taagtaaata catgcatatc
     2461 aggacgagct ctttttattc ctgtagcgac tgcaggagca cgaccatgag ctgcctctgc
     2521 catatcaaaa ttaaaatatt tataggcaaa aacaccacaa ccaacaggag ctacaccagc
     2581 tatttcattt attaatccat tttcatcgat tgcttcagca attaaccgat gagctacacc
     2641 atgcatacaa ccaggacaat aatggaaggg ttctttagta agtgcttcag gacgttttgc
     2701 gataatttcc attttttcct cctaccttta aaaacaagtt tccagttttt ctttgatttc
     2761 ggctggggta aaaacaactc cacctaattt tccccaaaaa tcaactggaa cctttccttc
     2821 tactgctatt tttacatctt ccaccatttg tcctgagttt agctcaaata catacacttt
     2881 tttcacattt ttacctattg cctgtttgat ggcatcatac ggatagggcc aaacccggat
     2941 aggacgaatc aagccaacac ttttcccttc tttcattact tcattgatgg ctgattttac
     3001 aactcggctg gcagttcccc aggcaacgca tagaacttca ttatcatcag atatattata
     3061 agtttcatag cgagcttcat tcttttcgat ttcagcatat ttcttatata aatctaaaac
     3121 atgcttttct aaaacctggg gatcaagttc cagagaattt atttcatgat ggtgtttttt
     3181 atcgccatct gcattgggac aaatacacca ggaaagatgt tgattggcaa gttcttgtag
     3241 ttccgcatca gtctttgccg gtttaaattc caccggttcc atcatctgac ctaacattcc
     3301 atcggcaaga atcataacgg gattacgata tttatctgcc aaatcaaatg cttcactggc
     3361 catatcagca aattcttgaa ctgaacttgg agccaaaaca attaaccgat aatcgccatg
     3421 gccgccacct ttagttgcct gaaaataatc accttgcgca ggttgaatat cacctaaacc
     3481 gggaccacca cgttgaacat taacaattac acagggaagt tcagctccag ctatgtaaga
     3541 gataccttcc atctttagag aaatacctgg tgaggaagaa gaggtcataa ctctttttcc
     3601 acttccagca gctccataaa ccatattaat ggcagcaact tcactttctg cttgaataaa
     3661 ggtaccattt accttcggca tcatttttgc catatattcg atcaattcac tttggggggt
     3721 gatgggatag gcaaaataca agcgacaacc acttcggata gccgcttctg caattgcctc
     3781 atttcctttc attaatattt tggacattca taacctcact tctcaatcgt tattgccaca
     3841 tcgggacagg ttatataaca tagcttgcag gcagtgcatt tttcctgctc aaaacactct
     3901 gcataattat agcctttggc attaatattt tccgataagc gaataatctt tctcggacaa
     3961 gcatttatgc acaagccaca acctttgcag taatctggat caacagtcat ttttggcata
     4021 tcgaatacct cgcttttttt attttttctc tttatgtgat aaagagattt tcagcggtat
     4081 tctgtcaaac tatttctttg ttgtgtatgg gctgtaaaag tagaaaatta cctccctctt
     4141 aagtttccat tcctattacg atacatataa atatcaatca caattgaaat gattaaagca
     4201 acaacaaaaa gatattgcga ttatttttct attgaaattg tattttacca acctcaacaa
     4261 tcacttttat aataaaatcc taatattgtc gtttcctatg ttccttgctg ggaggtttta
     4321 atctctcgtt taacatccct taatcaagcg ttaataatta acgcttgatt aaggttagat
     4381 aaacacttga ttaacgcttt tacctaagag cgagtagtag aagttgaact tgatatgcag
     4441 tttatacaaa ggtactaaac attttatggt aggtaaatat attaggatag taatcaatat
     4501 caacccataa ttttaaagtt aagaggtaaa tgagcttacc agttcttgct tggcaaataa
     4561 accgaagttt gctaacgaac tatgagcttg cccctcttta gagtaatttt attcagcacc
     4621 taagttgcgg tatatgtaca taaactatgt ggctttctta ttcggatttt tttgataaag
     4681 ggaaatttct gatgaattgt aaaaaagtta tattacttct tccttaggaa tgaaacttaa
     4741 taaaaagaga ggtacttttt ccctttactc cacttatctg tagtcgatat gtcttgactt
     4801 cttgcctacc gtgaatattg ttatttccat attgattttc ataatggttg cattccctat
     4861 gcatgtttca ataactccta tttatatatt tttggattta tcaccatttt ttctttgcga
     4921 tatttgtaat ctcctgtagg tagttcttat aactcttaca agccaatttt attaaaccgg
     4981 aacaaagaca tcttgtagtt ctatttgtaa cccatcttta ttgttattat gaattttacg
     5041 gtttccaaat aacaaatgaa aaataattga tgaggacatc aacctttcgg aatacagaaa
     5101 ttatttaatt gtaaaaatag catctgcagc ggatatataa tagcaaagta cctttgcctt
     5161 acatagtaat atagcttata attgctgtta cttaatcctt tctttaacga ttttttagcg
     5221 cttacttaac actgctttaa acccgttaag gtatcaataa agatatgtta aaggatcaaa
     5281 ataagtaaaa acaagagaca agtaacacat cgtataactg attgatatat aattatataa
     5341 accctgtaaa gaaaattttt tcttgacatt atcccccctg ctgaacagga tgttccacat
     5401 taaatgagca aaggactaat atgaagaaac ttaagaaaaa attaacagct tgctttagtg
     5461 cttttctatt gttatttata gcaccggtca tagctcagac cgtggcaagc gtgaacgagg
     5521 aagaattttt tactgatcga agtttgacgg taattgattc tttacaaacc caggaaccac
     5581 cgggagctcc tcctggtgaa aaaatgatcg ctacttatta ttctaaacga tttcatggta
     5641 gaaagacggc aagtggcgaa agatataatc gcgatgcctt cacttgtgcc cataaatccc
     5701 tcccctttca aaccctttta aaaataacta atcctcaaaa cggcaaatcg gtaaccgtgc
     5761 gtgtaaacga cagaggtccc ttcaatcgta atagagatat tgatctttct tatgcagccg
     5821 ctaaagaaat tgatatgctt gctgcaggag ttttacctgt ggaagtggaa attatgatgt
     5881 cgtcttatac agattcttta cttgcacaac attaatatag ttacctatcc ttgttgacac
     5941 cctgttatga ataacgacag gtaactacag atgcattcac tattaaatta ctcctctcgc
     6001 ataaagaaag cattagattt gggaaagccc attttggcta tggaatcaac tgttttaacg
     6061 catggtttgc cttatcccga caatttggat attttagaga cattggaaga gctctgttac
     6121 agtttggaag ttgaaccagc tacaataatt gtgctggatg gtcaacttca tatcggaatg
     6181 gatgatcaag aaaaggaaat cctgaaaaat aaactacagc attcacaaga actgaataaa
     6241 tttagtatgc gagatatacc ctatgcaata aattataaaa aaagcggagg gacaactgtt
     6301 tctgccacaa tgctgcttag ctataaagcc ggaataaggg tttttgctac aggtggaata
     6361 ggcggagttc ataaaagctg gaacgagaca ttggatattt cttctgactt aaaagccctt
     6421 tcggaaattc cgataattgt ggtttctgcc ggctgtaaag caattttgga tattccagca
     6481 acgctagaag ttttggaaag tgaagctgtt cctgtattgg gatggaaaac gaatgaattt
     6541 cctgcattct attcgcaaaa aagcggaaaa aagatagaca aaattgaaag cgctaaagat
     6601 gttgtaaata tctataaaac gatacaaaaa ttgtatccta atcccacagg aattttggtg
     6661 gctaatcctg taccggaaaa agatcaaatt ccttataagg aaatagccga acacattatc
     6721 gcagctattg atgaggcaaa agcaaaagga atttccggta aagaattaac tcctttcctg
     6781 ctggcaaatt tggcagaaaa aactgcaggc aaatcagttc agaccaatct tgctttgctg
     6841 aaaaataatg tactaatcgg cgcgcaaatc gccaaggagt taaaatgaaa atatatattt
     6901 ctactgatat ggaaggaatg ccaggaactt ttaactggga gcaggaaaaa accaaccgtc
     6961 cggaagttat gaaaaattat caggcacata taagtgactg cttgaatgcc attttgtata
     7021 gcggatttaa tgaattcata gaggaaatca ctgttgccga ttcccacagt ggaggagata
     7081 atttgagcta tgatataacc tctttggata aaagaattac gctaatttcc ggaggcccaa
     7141 gacctcaata tatgatgcct gcctttagta gcgaatattc tatggtcttt ttactgggtt
     7201 atcattctgg aaccggtgcc ctgaagggaa atatggacca cacttattct aacagcaaga
     7261 tttttaagat ctggataaac gatattccga tgaatgaagc aatcataaat tctgcttatg
     7321 ctggttattt aggtgtcccg gtggccttaa taagtggtga taaagctttg gaagaagaaa
     7381 taacaacggc tatgccctgg gtgcattttg ttagaactaa agaagcagtc gctaaatttg
     7441 ctgctaaaaa ttattcatct atcgtggtca aagaagctct cgtgaaagca gttcaagcat
     7501 ctttagctaa aaaagatata cctctatata catttcaaag ccctatacac cttaaaattg
     7561 agtttcactc tacctctatg accgattgcg ctgctctgat gcctcataca aaacgccttg
     7621 atggaagaac cattgagttc attgatgatg attatggagt tatctttgag gcaataatgg
     7681 ctttggtaac actggcttat gggtcaatcc tgtaaaaaag agatagtgac ttggtgagat
     7741 agaaattttg gaaagagata agtttcgttg ccaatataag aagtcgaggg atctggaagt
     7801 ttaaaaaaaa gaacatcgat aactggatcg gctaaattag ctggaacgat ataattacat
     7861 aaatataatc tgcgctctct gcgtaatcag cgtgagttac ttaagcaatc tgtgagaaac
     7921 gaaaaagggc gtagataact acgcccaata agtttacttt gtttcaatac cggctagcaa
     7981 agctcttaaa tttgcttcaa caatggcttc gcctttggga gcaaaaatcg ctttaattgc
     8041 ctcttcaatt tcttcttggc taaagcccaa atgagttatc gctgcaccca aaactacaat
     8101 attcatgctg cgattggctt tttgtgcatt agctaaagta tctgcgtcaa aaagaacata
     8161 attagggatt ttcttaattt ccctgtagat ttcttcttcc ggaggataat taggaatatt
     8221 tttaaagggg gtggaattag atataatcca gccgtcttct tttaaatatg gcagatagcg
     8281 taatgcttcc aagggctcta cgcttaaaat catatcggca gagccataag gaattagatc
     8341 actccaaata ggatcatcgg aaaggcgtag atgagattgc acatctcccc ctctttgact
     8401 cattccatgc acttctgctt gttttaaatt cattcctctt ttaatagcag cggttccaag
     8461 aatggtggct atggttaata taccttgtcc gccaactcct gcaagtatta tatccttttt
     8521 cattctaccg tctccatttt gttctttttc tttagggtct gaatgcattc tctacgtgca
     8581 ataacaacag aaacaccgtt gtatgcaagt tcttctttat agatttg
//
LOCUS       DAORHB010000040         8589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_7709_length_8589_cov_5.243145, whole genome shotgun sequence.
ACCESSION   DAORHB010000040 DAORHB010000000
VERSION     DAORHB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7709_length_8589_cov_5.243145"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1150
                     /locus_tag="PKM80_02200"
     CDS             158..1150
                     /locus_tag="PKM80_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV92747.1"
                     /translation="MIGKILTFARQGELEVEQVSVKEVLEDALPMIRATLPSNINIET
                     NIECERFVMADVTELQQVLINLASNAFHAMRPDGGTLTIKLNEISGFELIGIDPHIEL
                     ERDYLHLEVSDTGFGIAPEIINRIFDPYFSTKAAGEGTGLGLSIVHGIITSYKGFITV
                     HSIPGEGTSFHIFLPIVNAPKEAKKEEQPKTVYPYIPAKLMVIDDEPDLADIFHQALT
                     NAGYDVQSFNNSTVALEAFENNPDDFDLVIADVTMPQLDGMKLTAKIKALRNIPVILY
                     TGFSDHGVQQRATEAKVDRLLSKPLLPDELVAEVRRILYLISGGDINNNNPDYS"
     gene            1214..1591
                     /gene="gcvH"
                     /locus_tag="PKM80_02205"
     CDS             1214..1591
                     /gene="gcvH"
                     /locus_tag="PKM80_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNV92748.1"
                     /translation="MKIQDDLYYTETHEWVQVNGDIATIGISDFAQHELGDIVFVELP
                     EVGQKVSSGEPCGSIEAVKAVEDLVSPISGKVQEKNTDLEDSPELINKSPYEEGWLFK
                     VKISNQEELDNLLTAAEYQRMLP"
     gene            1620..2246
                     /gene="gmk"
                     /locus_tag="PKM80_02210"
     CDS             1620..2246
                     /gene="gmk"
                     /locus_tag="PKM80_02210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNV92749.1"
                     /translation="MIARNSSFLIILSAPSGGGKSTLVNEVLKVAKNIEYSVSYTTRS
                     PRGKEENGVHYHFVSEAEFLKRIDEGDFLEHARVFGNWYGTSKSFIKSRLAKNHHVIL
                     DIDVQGASLISATDIPYVKIFILPPSMQVLKKRLMLRATDSEEEIAKRIQIAKEEVTH
                     IPKYDYLVINDELEKAVQDVLAIIRAEENRVCRYSHPCEDFINIGENQ"
     gene            2243..2662
                     /locus_tag="PKM80_02215"
     CDS             2243..2662
                     /locus_tag="PKM80_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92750.1"
                     /translation="MIHEKIETFLEKADINYLFCLLSELEAYRLLQLPVYVRQKFPEK
                     ITVMAMNHVAENEVPDYISELAEAELAAMEQESPFIEEEGGEEEAEETVDDSTKFIED
                     FADSEGFKEEEDEDEDEEDEDEEDFYDYDNDSEDDDL"
     gene            2666..3397
                     /locus_tag="PKM80_02220"
     CDS             2666..3397
                     /locus_tag="PKM80_02220"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNV92751.1"
                     /translation="MKLNALHQYLEFLANSGIKELYFPGSDKRQRLNDLQIQYSTCSK
                     CPLHKNRHNFVYGEGNPDAIAMLIGEGPGEQEDLKGRPFVGAAGQLLDKMLAAIHINR
                     SEIYITNIVKCRPPGNRNPELEERLACLPYLVEQINIIQPKILLIMGLVAAQTLLGNN
                     NSLGWHREKIHYFMDIPVFVTYHPSALLRNSNWKRPAWIDLQEFEKEYRKILNEQTNF
                     DAKFPDKPNIATSNALTDSELPLSD"
     gene            3414..4238
                     /locus_tag="PKM80_02225"
     CDS             3414..4238
                     /locus_tag="PKM80_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV92752.1"
                     /translation="MCNSKIFPSSFSQRSLLLSVLFSLFLLVFSCLQARIAIYGDTRS
                     NPDIHKQIVKQIATFKPEAIFHTGDLNSSGKNQKEYDTFFQIISPLTQFGEFYPARGN
                     HEKDLSLFLKNFPYLNGSSYYTVMYDSIRFIILDSVLDLSPTSEQYCWLHEQLQEKIP
                     SLLILHHPVFSSGEHSDELGLQLFLPELLQQSSVKVVFSAHDHNYERSEYNNITYIVT
                     GGGGAPLREIYSHNPYSLVFSKQHHYIIAEREGPLINFKVYALSGDIIDTFLIGGF"
     gene            4241..5092
                     /locus_tag="PKM80_02230"
     CDS             4241..5092
                     /locus_tag="PKM80_02230"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HNV92753.1"
                     /translation="MPNPNVLAIHDLSGFGHTSLMAVIPIMYRMGIEVATMPSALLSA
                     NTDHPGYIIQDNSDLLEKSFQHWKELKLSFDAIYTGFLGSPKQVALLLDYLPKLKKNE
                     TITLVDPVLADEGKLYSCYTWEMVEAMRSLLAISNLITPNWTEFAFLSGYQTWEAIKP
                     EQTKERCRKLAAWGPRYIVVTSAPNSKGKFSSIVFYDADNDYYQELDCLYAPVNFPGS
                     GDCFSAMLLAGIINGYSIINSIKGAIAFMHKAIEISIPTVVNRITGINLAAALKNDPF
                     LFFSDKA"
     gene            5434..8454
                     /locus_tag="PKM80_02235"
     CDS             5434..8454
                     /locus_tag="PKM80_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNV92754.1"
                     /translation="MKKLAIVLGLALLVTGLFATFEWSDAVAIRQGVNIEWFRTGIET
                     TDGGAIYVWSDTKLGERDLWAQKVNASGDMVWGEPILIDGKLDRQEDPVITRTSDNNY
                     VIAWVDFSADLDGDIYAQKIDEQGNLLWQVGGVSVCSLVGMQISINMEPDNEGGVFII
                     WVDSRHPSKDLFGQHLSASGNALWTQNGIPIADGLGDEIQNTMLPDGQGGFIIAYTHS
                     YASNDDLYAKHFDANGTMTWPNPVVISNANGNQSDIRMAALTDGDFVVTWQDKRNTDP
                     DIYAQKINLAGDLLWGDYLVVYSDQDGLARPQLSPRIVKTSDNCAIIVWEDFSLDTQN
                     ADLFAQKISTTGTKLWSEEGVALCTAEFAQYGPRMAPDDNGGCYVVWDDLRNGNAPND
                     DIYAQHLSSTGVALWEANGKPICTAPNQQNGSLIKVSNNTVFINWMDIRNGSVGIYYQ
                     SLSSTGTPLLATNGVEVFWGLSGDTPLDNYLILKRSSDVVIIWQDTRFANDGYRIYFQ
                     FLNPDGSIGLETNGRPVTTSVGGTQSTPCAYVTPDDQIVIIWEDARGDNPKVYAQLIG
                     QNGERLWGDEGIPLTVSSPIRQKDPKVTYYNGSFYFGWSGSDQIGLNFFYHVYGQRIQ
                     NGQKMWGPDGLMISNLSQSDFNNECTLYDLIDNYYVWERYHPDNTRTVWVKRVAEDGS
                     SMDNWPEDGLRASTYNDYDTIQLLPKAHKTPDGIFVMWKDARGDFLQNYWGQHISDNG
                     NYLWNTIGVNLADSGREQELPTITVTDSGITFAWCEIIDGVNDIKAQKFSFTGSPLWG
                     DTGFFVVQRDSTQSTPFLASFPNGGYLISWEDYNIERDLYYKYIRENGDMIGNINGNV
                     LCDKAKSQYQPQIAILNNKAYCVWADGRSSGKTQILGLYAQKLGNGTPNIDEIAPSVQ
                     NMMLQQNYPNPFNPQTTINFTLKDNIHSLNLSIYNIKGQLVKTLYRGALQKGQYNLVW
                     DGTNDSGKQVSSGIYFYKLSNGKETQQKKMVLMK"
BASE COUNT         2719 a         1539 c         1728 g         2603 t
ORIGIN      
        1 ctaaaatcct taaatcctcg tttcgaaaat ccaatcctta aatccttgtt tctaatgata
       61 atctgcgtga tctgcagcca gaggcaaaat tagcgatccg caagcctctg aagctgatct
      121 gaatcaggca attaaagcct gcctgagagc caaaagtttg attggtaaaa ttctcacttt
      181 cgcccgccag ggagaattag aagtagaaca agttagcgtt aaagaagttc tggaagatgc
      241 tttaccgatg attcgagcta ctctgccttc caatattaat atcgagacga atatagagtg
      301 tgagcgcttt gttatggctg atgtaactga attgcaacag gttcttatca atcttgccag
      361 caatgctttt catgcaatgc gaccagatgg aggaactcta actattaagc taaatgagat
      421 atcaggattt gaactaattg gaatagatcc tcatatagaa ctggaaagag attatctgca
      481 tttggaagtt agcgatactg gctttggcat cgctcccgaa attatcaatc gtattttcga
      541 tccctatttt agcactaaag cagctggaga aggaacaggt ttaggcttat ccattgtcca
      601 tggtatcata accagctata aaggttttat cactgttcat agcattcctg gggaaggaac
      661 cagttttcat atcttccttc caattgttaa cgctcctaag gaagccaaga aagaggaaca
      721 accaaaaact gtttatccct atattccagc aaaattgatg gttatagatg acgaacctga
      781 tcttgcggat attttccatc aagcattaac taacgcagga tatgatgttc aatcctttaa
      841 taactctact gtagcattgg aagctttcga aaacaatccg gatgatttcg atcttgtaat
      901 agctgatgtt actatgcctc aactggacgg tatgaaatta accgctaaaa tcaaagccct
      961 aagaaatata cctgtgatcc tctataccgg tttcagtgat cacggagttc agcaacgcgc
     1021 tacagaggca aaagtagata gattgcttag taaacctctt ttaccagatg aactggtagc
     1081 cgaagttaga agaatcctat atcttattag tggtggggat ataaacaata ataatcctga
     1141 ttattcttga cataaaataa ccatccaaag atttgggcta tacaatatat aattctggta
     1201 aatggaggaa aaaatgaaaa tacaggatga tttatattac actgagaccc acgaatgggt
     1261 tcaagttaat ggcgatatcg ctactattgg tattagtgat tttgctcagc atgaacttgg
     1321 tgatattgtt tttgtggaac ttcccgaggt tggccaaaaa gtttcttctg gtgagccctg
     1381 tggttccata gaagccgtaa aagcagttga agatctcgtt agtcctatta gtggtaaagt
     1441 tcaggaaaag aatactgatt tagaagatag ccccgagctg attaataaat ccccctatga
     1501 agagggttgg ctattcaaag taaaaatatc caatcaagaa gaactggata atcttcttac
     1561 tgcagcagaa tatcaaagaa tgcttcccta acagtacatt ctgcagtggt ttaaattctg
     1621 tgatagccag aaatagcagc tttttaatta ttctctccgc tccttcggga ggaggaaaaa
     1681 gcactttagt aaacgaagtt ctgaaagttg ctaagaatat cgaatattct gtctcttaca
     1741 caactcgcag tccccgagga aaagaagaga acggagttca ttatcacttc gtctctgaag
     1801 ctgagttttt aaagcgaatt gatgagggag attttttaga gcatgctcgc gtattcggaa
     1861 attggtatgg aacttccaag agttttatta aaagccgctt agctaagaat caccatgtta
     1921 ttttggatat tgatgtgcag ggagcatctc ttatcagcgc taccgatatc ccctatgtga
     1981 agatctttat acttccacct tctatgcaag tattgaaaaa acggttaatg ctgcgggcta
     2041 cagatagcga agaagaaata gctaagcgta tacaaatcgc taaggaagaa gttactcaca
     2101 ttcccaaata tgattatctg gttattaacg atgagctgga aaaggcagtt caagatgttt
     2161 tagccattat ccgagctgaa gaaaaccggg tttgccgtta tagccacccc tgcgaggatt
     2221 ttattaatat aggagaaaat caatgataca cgaaaaaatc gaaacttttc tggaaaaagc
     2281 tgatataaac tatttattct gtcttttatc cgaattggaa gcatatcgtc tgcttcaatt
     2341 acctgtctat gtaaggcaga aatttccaga aaaaataacc gttatggcta tgaatcatgt
     2401 tgccgaaaat gaagttccgg attacatttc agaattggca gaagcagaac ttgctgcaat
     2461 ggaacaagaa agtcccttta tagaagaaga gggtggcgaa gaagaagctg aagaaactgt
     2521 ggacgattct accaagttca tagaagattt tgccgattca gaaggtttta aagaagaaga
     2581 agacgaagac gaagacgaag aagacgaaga cgaagaggac ttttacgatt acgataacga
     2641 tagtgaagac gacgatcttt aaccgatgaa gctaaatgcc ttacaccaat atttagagtt
     2701 tttagcgaac agcggtataa aagaactcta ttttccagga agcgataaac gacaacgctt
     2761 gaatgatctt caaattcaat attctacctg tagtaagtgc cccttacata aaaaccgtca
     2821 taactttgtt tatggagaag gaaaccccga tgccatagct atgttgattg gtgaaggacc
     2881 cggagaacaa gaagacctga aaggaagacc ttttgtagga gctgcaggtc agcttttgga
     2941 taaaatgctg gctgcaatac atattaaccg tagcgagatt tatataacta acattgtaaa
     3001 atgccgtcct ccaggaaatc gtaatcctga attagaggaa cgtctggcct gtttacccta
     3061 tctggttgaa caaattaaca ttattcaacc caaaatattg ttgattatgg ggttagtagc
     3121 agcacaaact cttttgggaa acaacaattc tcttggctgg catagagaaa aaattcacta
     3181 ttttatggat attcctgttt ttgtaaccta tcatccttct gctctcttaa gaaactctaa
     3241 ttggaaaaga cctgcctgga tagatttaca ggaattcgag aaagagtatc gcaagatctt
     3301 aaatgagcag actaatttcg atgcaaaatt tcctgataaa ccgaatatcg ctacttccaa
     3361 tgcactcact gatagtgagt tgccattatc tgattaagct aagataataa ataatgtgca
     3421 acagcaaaat ctttccgtct tcattttctc agcggagttt gcttttatct gttttatttt
     3481 cattattcct cctggtattt tcctgcttac aagctcgtat tgcaatttat ggagatacaa
     3541 gaagcaatcc tgatattcat aaacagatag ttaaacaaat tgctactttt aaaccggaag
     3601 ctatttttca tacaggagat ttgaatagct ctggaaaaaa tcagaaagaa tatgatactt
     3661 ttttccaaat catttcaccg ctaactcaat ttggtgaatt ttatcctgcc agaggtaacc
     3721 acgaaaaaga tctctctcta tttttaaaga attttcccta tctgaatggc agctcatact
     3781 atactgttat gtatgatagc atccgtttta ttattttaga tagtgtttta gacctctccc
     3841 ctacctcaga acaatattgt tggttacacg aacaattaca ggaaaagatc ccttcgctgc
     3901 tgattttgca tcatccggtt ttttcttctg gagaacatag tgatgagctt ggtctccagc
     3961 tttttctccc tgaactttta caacaatcct cagtgaaagt tgtttttagc gctcacgatc
     4021 acaattacga acgctctgaa tataacaaca taacctatat tgttacaggt ggagggggag
     4081 cacctttacg cgagatttat agccacaatc cctattcact tgtttttagc aaacaacatc
     4141 attacattat tgccgagcgc gaggggccct taattaattt taaggtttat gccttatccg
     4201 gcgatattat tgatactttt ttaattggtg gattttagat atgcccaatc ctaatgttct
     4261 tgctattcat gatttatctg gttttggcca tacctcttta atggctgtta tacctattat
     4321 gtatagaatg ggtatcgaag tggctactat gccaagtgct ttgctttctg ccaatacaga
     4381 ccatccagga tatattatac aagataatag cgatctttta gaaaagtcct ttcagcattg
     4441 gaaggaacta aagcttagct ttgatgctat atatacagga tttttaggtt cgccgaaaca
     4501 agtagctctg ttgcttgatt atttaccgaa acttaaaaaa aatgagacga ttactttggt
     4561 tgatcctgtt ttggctgacg aggggaaatt atacagttgt tacacctggg aaatggttga
     4621 agcaatgcgt tcattacttg ccattagtaa tctcatcact cctaattgga cagaatttgc
     4681 ttttttgagc gggtatcaaa cttgggaagc tattaaacct gaacaaacca aagaacgctg
     4741 tagaaaatta gctgcttggg gacctcgtta tatcgtggtt actagtgcac ccaattctaa
     4801 aggcaaattt tcctctattg ttttctatga tgcagataat gattattatc aagaattgga
     4861 ctgcctttat gcccctgtta attttcctgg ttccggagat tgtttttctg caatgctttt
     4921 agcagggatt atcaatggct atagtattat caattccatc aagggagcaa tcgcttttat
     4981 gcataaggca attgaaataa gcattcctac agtggttaac cgcataaccg gtatcaattt
     5041 ggctgcagcc ttaaaaaatg atcctttcct tttcttctcc gataaagctt agaaaagaaa
     5101 tatatcatat tataccaaga tgtcttaatg gagatcttgg tttttttttg attatttatt
     5161 ttatgattaa tgaggcttat tcagttcttt aaaaccttgt ctcctaaaca aaatcaacat
     5221 accagagttg catattagat atcctcttag tagataataa ctatttgttt ggaaacaatc
     5281 ttgcattaat atttatagat atggagataa taattcgttt tcacaaatat gaatggggtt
     5341 ctgattatgc tttgctttat attttataga aaaggaccta aatgtataga tgttatggcc
     5401 acataatgtt atataagata aggagataga gaaatgaaaa aattggcgat agtgcttggt
     5461 cttgcccttt tagttacagg gctttttgcc acttttgaat ggagcgatgc agtagctatc
     5521 cgtcagggtg taaatattga atggtttcgc accggtattg aaactaccga cggaggagct
     5581 atttatgttt ggtcagatac aaaacttggc gagcgcgatc tttgggctca gaaagttaat
     5641 gcctcaggag atatggtttg gggagaacca attctaatag atggcaagtt agaccgtcaa
     5701 gaagaccctg taataaccag aacaagtgat aataattatg ttattgcctg ggtagatttt
     5761 tctgcagatt tagatggcga tatctatgct caaaaaatag atgaacaggg taatttactt
     5821 tggcaagtag gcggagtttc agtttgttcc ttagttggaa tgcagatttc gataaatatg
     5881 gaacccgata atgagggagg agtgtttatt atttgggtgg acagtcgcca tcccagtaaa
     5941 gacctttttg gccagcatct ttctgcaagt ggcaatgctc tatggacgca aaatggtatt
     6001 cctatcgccg atggactggg agatgaaatt caaaatacaa tgcttcctga tggacaggga
     6061 ggatttatta ttgcatacac ccacagctat gccagtaatg atgatctcta tgctaaacat
     6121 tttgatgcca atggaacaat gacttggcca aatcctgttg ttattagtaa tgcgaatgga
     6181 aaccaatctg acattaggat ggctgcttta actgatggcg attttgttgt tacctggcag
     6241 gacaagagaa ataccgatcc cgatatttat gcccaaaaaa tcaacttagc cggagatttg
     6301 ctctggggag attatcttgt tgtttatagt gatcaggacg gcttagcaag gccgcaattg
     6361 agtcccagga tagtaaaaac ctctgataac tgtgctataa tagtttggga agattttagc
     6421 ttagatactc aaaatgctga tctctttgcc caaaaaatat caacaaccgg cacaaaactc
     6481 tggtcagaag aaggtgttgc cctttgtaca gcagaatttg ctcaatatgg acctcgtatg
     6541 gctcctgatg ataatggtgg ttgttatgta gtttgggatg atttacgaaa tggtaatgca
     6601 cccaatgatg atatatatgc tcaacatcta tcttcaactg gagttgcctt atgggaagca
     6661 aatggaaagc ctatttgcac tgcacccaat cagcaaaacg gaagcttaat aaaagttagt
     6721 aataacaccg tttttatcaa ttggatggat attcgcaacg gcagtgtagg tatatactat
     6781 caatcactat cttctacggg aactcctctc ttagctacaa atggagttga agttttttgg
     6841 ggtttgagcg gagacacacc tctggataat tacttaattt taaagcgtag ttcagatgta
     6901 gtaattattt ggcaggatac ccgttttgcc aatgacggtt ataggatata tttccaattc
     6961 cttaatccag acggtagtat aggtttggaa acgaatggac gcccagtaac aacttctgtg
     7021 ggaggaactc aatccacacc ttgcgcttat gtaactccag atgatcaaat tgttattatt
     7081 tgggaagatg ccagaggtga taatccaaaa gtttatgccc agctgatcgg acaaaatgga
     7141 gaaagattat ggggagacga aggtattcct ttaactgtaa gtagcccgat aagacagaag
     7201 gaccctaaag taacttatta taacggttcg ttttactttg gatggtcagg ttcagaccaa
     7261 attggattaa atttctttta ccatgtttat ggtcagcgga tacaaaatgg acaaaaaatg
     7321 tggggaccag atggattgat gatttccaat ctctctcaga gtgatttcaa taacgaatgt
     7381 actctctatg atcttattga taactattat gtttgggaga gatatcaccc cgataatact
     7441 agaacagttt gggttaaaag agttgcagaa gatggttcat ctatggataa ttggcctgaa
     7501 gatggattac gagcttctac ttataacgat tatgatacta tccaattgct tcccaaagct
     7561 cataaaactc cagacggtat ctttgtgatg tggaaagatg cccgaggtga ttttcttcaa
     7621 aattactggg gacagcatat ctcggataat ggaaactatt tatggaatac tataggcgta
     7681 aatcttgctg atagcggaag agaacaagaa ttaccaacaa tcacagtaac cgatagcggt
     7741 atcacttttg cctggtgtga aataattgat ggtgttaatg atattaaggc tcagaagttc
     7801 tcttttacag gatcaccttt atggggcgat acaggtttct ttgttgtaca acgagattcc
     7861 actcaatcca caccattttt ggctagtttc cccaatgggg gttaccttat cagctgggaa
     7921 gattataaca tcgaacgcga cctctactat aaatatattc gtgaaaatgg cgatatgatc
     7981 ggaaacatta atggcaatgt cctctgcgat aaagccaaat ctcaatatca acctcaaata
     8041 gccattttga ataataaagc atattgtgtt tgggcagatg gacgatccag tggaaaaact
     8101 caaattttgg gactctatgc ccaaaaacta ggtaacggaa cccccaatat cgatgagatt
     8161 gctccttctg ttcaaaatat gatgctacag caaaattatc ccaatccctt caatccgcaa
     8221 accactatta actttacttt gaaggataat atccactccc taaacttaag catctataat
     8281 ataaaaggac aattggtcaa aactctttac cgcggagccc tgcaaaaagg ccaatataac
     8341 ttagtgtggg atggaaccaa cgattccgga aaacaggtta gcagcggaat atatttctat
     8401 aaactaagca atggaaagga aacacaacag aaaaaaatgg tgctgatgaa ataataaccg
     8461 tgaactgcat ataaaaaata tatgcaataa gtgaatagcg tgttagatta acatagggaa
     8521 ttatattata agagtcccca ttgccataat tggttgttac atatagtacg aactttgggc
     8581 agttgggac
//
LOCUS       DAORHB010000041         8462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_7918_length_8462_cov_3.336981, whole genome shotgun sequence.
ACCESSION   DAORHB010000041 DAORHB010000000
VERSION     DAORHB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7918_length_8462_cov_3.336981"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKM80_02240"
     CDS             <1..491
                     /locus_tag="PKM80_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92755.1"
                     /translation="KATPDPGALTKYKIQDGNKTLFFYNVFAANDPQSTVGIIKMVTG
                     HLQNVEKIIILNSRADRLFRSHQLVDAVKDLDFSYLLLTGEIPEKVESYCLQAGIPKE
                     KIIPLGEPLPEVIYKKVIELTNSESHILGIGNIAGDIKYGAQIVAHFKHKMKENIERS
                     RM"
     gene            488..943
                     /gene="pgsC"
                     /locus_tag="PKM80_02245"
     CDS             488..943
                     /gene="pgsC"
                     /locus_tag="PKM80_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HNV92756.1"
                     /translation="MIDTIVQAAVGMGVIVSLMFSELLGASAGGIVVPGYIALYLDKP
                     LQIIGTLIISLITWGIIRIISSFTLMFGKRRMVLSILIGFILGWASRIFVFQDITVHQ
                     LQMQSIGYIIPGLIANWFERQGFWKTVSSMGIAAILVRLALMVIFNGEI"
     gene            946..2067
                     /gene="pgsW"
                     /locus_tag="PKM80_02250"
     CDS             946..2067
                     /gene="pgsW"
                     /locus_tag="PKM80_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HNV92757.1"
                     /translation="MYRPSLKSDRSLIILLILAIVLFYIAQSSYLHIKSDNYELKIAA
                     ANLMKASIDSLTAEINRRGIEIDPIDDPLKTGLIGNRLSSITTDRGLLSEKQAAINPN
                     ISAIFVEELSKLKLMPGDCIAVGITGSNPAVNIALYSAIQVMELNPKIIVSLSSASYG
                     ANRPELTWLDMENILKEKGLINFKSAYASFGGKEDLGIGLSDNGIKALRDAMQRNNVP
                     LLIGNNLDDNISLRMAAYDEMLPEGQRYKAFINIGTGIANVGSEPNANLIPEGINRNL
                     AEHSYEKEGVMIKMAKNNIPVLHFRRILRWAKRYGLPTSFDTIPKVGQGKMFSSLVHN
                     QTVNIICLAILVIAIAMVIIFDRHDRRFMANIVDPDEEL"
     gene            2080..2901
                     /locus_tag="PKM80_02255"
     CDS             2080..2901
                     /locus_tag="PKM80_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92758.1"
                     /translation="MHKHHFSIILIILFANILFAQTANRYKVLPFDNPGTKRLLKTET
                     GNYYYYRSLPERALILNTTGVDKIEIRSFSYGEVRKPEIVSVIGKERKVYQLSLKEKK
                     GDYYIYNPFTLDIPEGTKEIKVICYDRSIYMRAFSVLPPKPAKPKKQPNLVIKAHSGV
                     VSVQHNSSSTDYYSFMPDQPFKFTINNGRNAYVYIRPRLLDRSLPQLGVYVNGELVEV
                     IDFTYKRTTKYHSQGINNLGIAKKIVLPENTNSTNYELRALSDHLFLAKPVLLKK"
     gene            2911..4062
                     /locus_tag="PKM80_02260"
     CDS             2911..4062
                     /locus_tag="PKM80_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92759.1"
                     /translation="MSNSNSKIPSVDTKPDMIKELKEIVDSNESSSSTMQEKRNIPKK
                     HKIRRKTSIRPIIMLLSLCLFLGFIDIDAQITASVKIGATYSDNVFHLSEYDINRFDD
                     HHPNLDFVDTMDDLTLSTRIELGYPMSYRWWKFTPSITSTLSQNISNEDKYRYDWAAK
                     FRVDRYYWNCSIQYASSPYIYFRHFIDTDGTKELEKYNYSRDTFRGDLAVKPFPKTTL
                     KADLRYDIYRYNEYFTEADGKAFAEELGVSYKFPYFTIEGSYAYRDFSCDKRIENKDC
                     SYQSNTYKGKIVLPKMPLESKSETLWQPSLTLNYEQKYYQGGGSWYGGRADYTYTLSA
                     GMNILFSPKWNLSLDYSHLFRNVETDNESVLRLKEYNENRLSAALRFKF"
     gene            4078..4413
                     /locus_tag="PKM80_02265"
     CDS             4078..4413
                     /locus_tag="PKM80_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92760.1"
                     /translation="MVFKKESDPKKIKEFLTLVENPKVELRRYKRPVLVWAVQEGTVY
                     YSFWEDDLLSRYGLENVDGWDFEEDLLFCPDWVEDSDEEDLDDIDLDEDDIEELNRFF
                     HINHDDKDK"
     gene            4413..5420
                     /locus_tag="PKM80_02270"
     CDS             4413..5420
                     /locus_tag="PKM80_02270"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNV92761.1"
                     /translation="MRIAILGGGGWGLALSALLSENGHQVLVWEFNPVYITLLKETHS
                     NPHLLPEITIPGEVDFSNDFKDIVIFSPEIVILATPSQFLRTTLQNTPDSLWHSPSLK
                     AIVNVAKGIEEDSLKTLDIVIKDELPFFPENKICALSGPSHAEEVARKIPTTVVIAGS
                     DEDLLCQLQIVFSNSYFRVYRNSDLVGVEIGGAVKNIISIAAGIIYGLNFGDNTIGAL
                     LTRGIVEIQRLGITLSARPETFLGLSGLGDLITTATSPHSRNRYVGMEIGKGKKLFDI
                     ISQMEMVAEGVTTTRSVYLLAKKMQVEMPIVEKVYQVLYEDEDPRKAIVELMTRELKA
                     E"
     gene            6025..>8462
                     /locus_tag="PKM80_02275"
     CDS             6025..>8462
                     /locus_tag="PKM80_02275"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNV92762.1"
                     /translation="MNIRWFICGFILLYANIAIASIRYTTLENFESGSIILSSWSDDD
                     VQPDAWTLSTSTPDGSSYCLQLTGNCYKLQQITPYPIDSTNVFQIQVKTTSNPCIQGI
                     GFTDGMHSLFYSFSGTRILDIEVWIPVYQGAFINNVWNTIQLPISNDWQAFWGYHPII
                     NGIIYINDLDGLSSRSVWFDNLIDISSDLPVAPVVSISTSYQVKDTGVFFYNDVIDPD
                     SDVFTYEWSFGDSTYSNLPNPYHLYTIGSSYPYTVTLKVTDDTGKVGFASTDIILETG
                     NSNLPVTCNFVGDIMLARRYENPGGIIPTLGVNAIFNPTKPYFGDAADINIANLEVVL
                     SNVGVHHPTKSVYYRGNPANVSGLVYAGIDIVCTANNHTMDYGIEAYQQMQGFLDSAG
                     IIYSGCGANAYEAYLPAFYNCRGINIAFLRSSDRTGQYNNAQPFLQAGYCKPGFAYMT
                     PYYIQQQIQAVEGIADLKIVEMHGGSEYSLAPGAGYDKELNPFLEDTEDEDYCYRNDV
                     PHQWDREIRHSAIDSGADLVIVHHPHIIQGLELYQNKLIAHSLGNFVFDLDYPETMPT
                     MILYADAYLDGFRNFRIKPFYIDQFIPKPATGQLAIYILDYIASRSRDLDTRVSVDKI
                     ALEAQVLMDDDYPPVVANAFSCHKQLQNLNENMSCTPPIKLPRRGSICEITNVEPPAE
                     YQFRLGQENIWFGNFEDEGSTLWVPPDYSTSDFIDGTRSAKLSTTSSSTVTSTISNRC
                     KIYDNSKKYTLHGWIKTENVYSANIMIRFYTSRTSGSSSYTDYVIDNLSGTTEWTYYF
                     KELNLQSNVYYYDIR"
BASE COUNT         2758 a         1369 c         1615 g         2720 t
ORIGIN      
        1 agaaggcaac tcctgatcct ggggctttaa cgaaatacaa aatacaggat ggaaacaaaa
       61 ctctattttt ttataatgtc tttgccgcta atgatcccca atccacagta ggaataatta
      121 agatggttac tggtcatttg cagaatgtgg aaaaaattat aattttgaac agtagagcag
      181 atcgtctctt tcgtagccat caattagtgg atgctgtgaa agatctcgat ttctcttatc
      241 ttttactaac tggagaaatt cctgaaaaag ttgaatctta ttgtttgcaa gccggtattc
      301 ctaaagaaaa aatcatccct ttaggtgaac ctttaccaga agttatttat aagaaggtta
      361 tagagcttac taacagcgaa tcccacattt tgggaatagg aaacatagca ggcgatatta
      421 aatatggggc acagatagtt gctcatttca agcataaaat gaaagagaat atagaaagga
      481 gccggatgtg attgatacaa tagttcaagc cgcggttgga atgggggtaa ttgtctcact
      541 aatgttcagt gaattacttg gtgcttctgc agggggtata gtagtaccag ggtatattgc
      601 cttgtatctg gataagcctt tgcagataat aggcacccta attattagtt taatcacttg
      661 gggaattatc cgtataatta gtagttttac tcttatgttt ggtaaaaggc gtatggttct
      721 aagtatatta ataggtttta tcctaggatg ggcctcacgc atttttgtat ttcaagatat
      781 taccgttcac cagttgcaga tgcaatctat aggttatatc ataccaggac ttattgccaa
      841 ttggtttgag cgtcaaggtt tttggaaaac cgtatcttca atgggtatag cagcaatact
      901 tgttcgactt gccctgatgg taatatttaa tggagagata taattatgta tcgcccttca
      961 ctgaaatctg atcgttccct gataattcta ttgatattag ccattgtgtt attttatatt
     1021 gcacaaagca gttatctaca catcaaatca gataattatg aattaaagat agctgctgcc
     1081 aatttgatga aagcatcaat tgattcgtta actgcagaaa taaatcgacg agggatagaa
     1141 attgacccga tagatgatcc gttaaaaacc ggtttaatag gaaaccgtct tagttctatt
     1201 acaacagata gaggtctatt gtctgaaaaa caagctgcta taaatcctaa tatatctgct
     1261 atctttgttg aagaactatc caaactgaaa cttatgcctg gagattgtat agctgtagga
     1321 ataacgggaa gcaatcctgc tgtaaatatt gctctgtatt ctgcgataca agtaatggaa
     1381 cttaatccca aaataattgt ttctttatct tcagcttctt atggagctaa ccgtccggaa
     1441 cttacatggt tagatatgga aaacatatta aaggaaaaag gattaattaa ttttaaatct
     1501 gcctacgcct cctttggggg aaaagaagat ttgggaattg gtttatccga taacgggatt
     1561 aaagcactac gagatgcaat gcaaagaaat aatgttcctc tattaatagg taataatctg
     1621 gatgataaca tttctcttag aatggccgct tatgatgaaa tgttaccaga aggtcaacgc
     1681 tacaaagcat ttataaatat tggaaccggt atcgccaatg tgggtagcga acccaatgcc
     1741 aatctcattc cggaaggtat caaccgaaat ttagctgaac atagttatga aaaagaaggg
     1801 gtgatgatta aaatggcgaa aaacaatata ccggtattac attttcgccg tattttacgt
     1861 tgggctaaaa gatatggtct tcctacttct tttgatacta taccaaaagt gggacagggg
     1921 aaaatgttta gttcacttgt tcataatcaa accgtcaata ttatttgtct ggctatctta
     1981 gttattgcta ttgcgatggt aatcatattt gatagacacg atcgcaggtt tatggctaac
     2041 atagtagatc cggacgaaga actgtaaggg ataataaaaa tgcataaaca tcatttttct
     2101 atcatactaa tcatcctttt tgccaatata ctctttgcgc agacagcgaa tcgatataag
     2161 gttcttcctt tcgataatcc aggcactaaa cgccttctta aaacagaaac aggaaactat
     2221 tattattacc gcagtttacc tgaaagagca ttaatactaa atactaccgg agttgataaa
     2281 attgaaatcc gcagttttag ttacggcgaa gtgcgtaaac ctgaaattgt ttctgttatt
     2341 ggtaaagaac ggaaagttta tcaactatcg ttgaaagaaa agaagggcga ttattatatc
     2401 tataatccct tcacattaga tattcctgaa ggcactaaag agataaaagt aatttgctat
     2461 gaccgctcta tatatatgag ggcttttagt gttttaccac ctaaacctgc caaacctaaa
     2521 aaacaaccga accttgttat aaaagcacat agtggagtgg ttagtgtgca gcataatagc
     2581 tcaagcacag attattatag ctttatgcct gatcaacctt ttaagttcac tattaataat
     2641 ggtcgaaatg cctatgttta tattcgtcct cgtttattgg ataggtcttt acctcaactg
     2701 ggagtttatg taaatggcga attggttgaa gtgatagatt ttacctataa gcgcactaca
     2761 aaatatcata gccagggtat caacaacttg gggattgcca aaaaaatcgt tttaccggaa
     2821 aatactaaca gtaccaatta tgagttgcgt gctctaagtg atcatttatt ccttgcaaaa
     2881 cctgttctat tgaagaagta gagataaatt atgtcgaatt caaactctaa aattccttcc
     2941 gttgatacaa aacctgatat gattaaagaa ctaaaagaaa tagtagattc aaatgaatcc
     3001 tcttcatcaa cgatgcaaga gaaaagaaat ataccaaaaa agcataaaat aagaaggaaa
     3061 accagtatcc gacctattat tatgctgctt tctttatgcc ttttcttagg cttcatagat
     3121 attgatgcac aaattacagc ttctgtaaag attggagcta cttattcaga taatgtattt
     3181 catctatctg aatatgatat taatcgtttt gacgaccatc acccgaatct ggattttgtg
     3241 gatacaatgg atgatttaac tcttagcact cgcattgaac tgggttatcc tatgtcttat
     3301 cgttggtgga aatttacccc ttctataaca agtactctat ctcaaaatat tagtaatgaa
     3361 gataaatatc gctacgattg ggcagctaaa ttcagggtgg atagatatta ttggaattgc
     3421 agcattcagt atgcaagcag tccttatatc tactttcgtc attttattga tacggacgga
     3481 actaaagagc ttgaaaaata caattatagc cgggatacat ttcggggaga cctagctgta
     3541 aaaccatttc ctaaaacgac actgaaagca gatctgcgct atgatatata tcgttataat
     3601 gaatatttca ccgaagctga tggtaaggct tttgctgaag aattgggcgt aagttataaa
     3661 tttccctatt ttactataga aggttcttat gcctatcgag atttcagctg cgataaacga
     3721 attgaaaata aagattgctc ctatcaaagt aatacctaca aaggaaagat tgttttaccg
     3781 aaaatgccgc tggaatcaaa aagtgaaact ctttggcaac cttcactaac actcaattat
     3841 gaacagaaat attaccaggg aggaggttct tggtatggtg gtagagccga ttatacttat
     3901 acattatcag ctggaatgaa tatattattt tcaccaaagt ggaatttatc tcttgactac
     3961 tctcacttat tcagaaatgt agaaactgat aatgaatcag tgttgcgttt gaaagaatat
     4021 aatgaaaatc gtttaagcgc tgctctcaga ttcaaatttt gagcatagag gtttaaaatg
     4081 gtatttaaga aggaaagcga tcctaagaaa ataaaggaat tcctaacttt agtggaaaat
     4141 cccaaagtgg aattgcgacg ctataaaaga cccgttctgg tttgggctgt tcaagaggga
     4201 actgtatatt attcattttg ggaagatgac ctcctatcac ggtatgggct ggaaaatgtt
     4261 gacggttggg atttcgaaga agaccttctt ttctgtccgg actgggtaga agatagcgac
     4321 gaagaagatt tggacgatat tgatctggac gaagatgata ttgaagaatt aaaccgtttc
     4381 tttcatataa atcatgatga taaggataaa taatgcgtat agctatcctt ggaggtgggg
     4441 gatggggact agcattatca gcattgctat cagaaaatgg acatcaagtt ttagtttggg
     4501 aatttaatcc tgtatatata acactgttaa aagaaaccca ttccaaccca catctgttac
     4561 cagaaatcac tattcctgga gaagttgatt tttctaatga ttttaaagat attgtcattt
     4621 tttctccgga aatagttatc ttagctactc cttcacagtt tttaagaaca accttacaga
     4681 ataccccaga ttctttatgg catagcccat ctttaaaagc gattgtgaat gtagcaaaag
     4741 gtatcgaaga agacagtttg aaaacccttg atatcgtaat aaaagatgaa ttgccttttt
     4801 ttccagaaaa caagatttgt gctttatccg gtccttctca tgcagaagaa gttgccagga
     4861 aaattcctac aacggtagtt attgcgggta gcgatgaaga tttactttgc caattacaaa
     4921 ttgtttttag taattcttat tttcgagtat atcgtaattc agaccttgtc ggagtggaaa
     4981 ttggaggtgc tgtaaaaaac ataatctcca tagctgcagg tataatttac ggtttgaact
     5041 ttggagataa cacaatcgga gcattactaa ccagaggaat tgtggaaatt caacgtttgg
     5101 gaattacttt atcagcgcgt ccggaaacct ttttaggact ttcaggtttg ggagatttaa
     5161 taactacagc tacttcacct catagtagaa ataggtatgt tggaatggag attggaaaag
     5221 ggaagaaact atttgatatt atatcacaaa tggaaatggt ggctgaagga gttactacta
     5281 cccgcagtgt ttatctgctt gctaaaaaaa tgcaagtgga aatgcctatt gttgagaaag
     5341 tatatcaggt tctttatgaa gacgaagatc ccagaaaggc aattgtagaa ttaatgactc
     5401 gcgaactgaa agcagaataa aagggctgat taatttcatc ttagagatta gttaaagatt
     5461 attatattcg gataatattc tataccagtg tcttattatt gaatgattag gtataggttt
     5521 ctccaaacga taatgatgaa aacaaggaaa tgttctcaaa gtttacttat accagatagc
     5581 ttaatttgga tatgtgaaag cgaaatggta ttagccactc ttccatctga aaattgcaga
     5641 gccgatatca gaactggatt agcataatac gaatcataac ttctttaggg ttatagataa
     5701 tctctatatt ggataaggac atagtttact tcttcataat aagctcttta aaaaatatgg
     5761 aaccatttat gatatagatt gcccaagaca gaatgatacc tttttattgt ctattttatc
     5821 ttctacgatc cttgtggaga actaatccca gttttcaata gtcagcagat aagacattat
     5881 ataatattag cttatataat attaatattg gcttacgggg gaggcaaatg aaagaaaaca
     5941 aatacgcttg tctggcttaa aaaatgcttt attttatatt ctgaatatag caataagcaa
     6001 taaatagggg aaggggagga tagaatgaat ataagatggt ttatatgtgg tttcatcctt
     6061 ttatatgcaa acattgctat agcttctatt cgctatacaa ccttggaaaa ttttgaaagt
     6121 ggttcgatca ttttgagttc ttggagtgat gatgatgttc aaccggatgc ctggactctt
     6181 agtacatcaa ctccagatgg tagttcctac tgtttacaat taaccggtaa ctgttacaaa
     6241 cttcagcaaa taactcccta tcctatagac agcaccaatg tttttcagat acaagttaaa
     6301 actacatcaa atccctgtat tcagggcata ggttttacag atggtatgca tagtttattt
     6361 tatagctttt ctggaacgag aatattggat attgaggttt ggattccagt atatcagggt
     6421 gcttttatta ataatgtttg gaacacaatt cagcttccta taagtaatga ttggcaggcg
     6481 ttttggggtt atcatccaat tatcaatggg atcatttata tcaatgattt agatggcctt
     6541 tcttcgcgta gtgtctggtt tgataatctc attgatatta gttctgatct tccggttgct
     6601 cctgtggtaa gcatatctac atcctatcaa gtaaaagata caggtgtatt tttttacaac
     6661 gatgtaattg atcctgatag tgatgttttt acctatgaat ggtcttttgg ggattctaca
     6721 tatagcaatt tacccaatcc atatcatcta tatacaatag gatcttctta tccctatact
     6781 gtaactctaa aggtgactga tgatacagga aaagttggtt ttgcttcaac tgatattata
     6841 ttggaaacag gtaactcaaa tttacctgta acctgtaatt ttgttggaga tataatgctt
     6901 gcaagaagat atgaaaaccc gggaggcatt atacccactt taggagtaaa cgctattttt
     6961 aatccaacca aaccttattt tggtgatgca gcagatatta atatagcaaa tttggaagtt
     7021 gttttatcta atgttggggt tcatcaccca acaaaaagtg tttattatag aggtaatcct
     7081 gctaatgtaa gtggtttggt ttatgcagga attgatattg tttgtacagc caataatcat
     7141 actatggatt atggaattga agcatatcaa caaatgcaag gttttcttga tagtgcaggt
     7201 attatatata gtgggtgtgg agcaaatgcg tatgaggcat atcttcctgc attttataac
     7261 tgtagaggta taaatattgc cttcttaaga agttcagata gaacaggcca atataataat
     7321 gctcaacctt ttttacaggc aggttactgt aaacccggct ttgcttatat gactccttat
     7381 tatatacagc aacaaattca agcagttgaa ggtattgccg acctcaaaat tgttgaaatg
     7441 catggaggta gtgaatattc tttagctcct ggcgctggat atgacaaaga gcttaatcct
     7501 tttttagaag acacggaaga tgaggattat tgttatcgga atgatgttcc tcatcaatgg
     7561 gatagagaaa ttcgtcatag tgccattgac agtggtgccg atttagtaat agttcatcat
     7621 ccacatatta ttcaaggtct ggaactatat caaaataagc tcattgctca ttctttggga
     7681 aactttgtat ttgatttgga ttatccagaa acaatgccta ctatgatact ctatgcagat
     7741 gcttatttag atggctttag aaatttccga atcaaacctt tttatattga tcaattcatt
     7801 cctaaaccag caactggaca gcttgctatt tatattttgg attatatagc cagcaggtct
     7861 cgtgatcttg atacccgtgt ttcagtagat aaaatagcat tggaagccca agttttaatg
     7921 gatgatgatt atcctcctgt agttgccaac gcttttagtt gtcataaaca gctgcaaaac
     7981 cttaatgaaa atatgagctg tactccacct ataaaacttc ctcgaagagg atctatatgc
     8041 gagataacaa atgttgagcc tccagctgag tatcaatttc gtctaggaca ggaaaatatc
     8101 tggtttggta attttgaaga tgagggttct actctttggg ttccacccga ttattccaca
     8161 tcagatttta ttgatggaac ccgtagcgca aaacttagca caaccagttc ttctactgta
     8221 acttctacaa tttctaatcg ttgtaagatt tatgataaca gcaaaaaata cactcttcat
     8281 ggatggataa agaccgaaaa tgtttatagc gctaatataa tgataagatt ttatacttcc
     8341 agaacttctg gttcatcatc ttatactgat tatgttatag ataatctatc tggaacaact
     8401 gaatggactt attattttaa agaactaaac cttcaatcaa atgtctatta ttatgatatc
     8461 cg
//
LOCUS       DAORHB010000042         8400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_8007_length_8400_cov_4.892750, whole genome shotgun sequence.
ACCESSION   DAORHB010000042 DAORHB010000000
VERSION     DAORHB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8007_length_8400_cov_4.892750"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /gene="murJ"
                     /locus_tag="PKM80_02280"
     CDS             <1..1470
                     /gene="murJ"
                     /locus_tag="PKM80_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNV92763.1"
                     /translation="AFFFGTTYLNDAFNVGYNIPNLLRRLFGEGALSTAFVPIYNEIL
                     IEDGKDKQIDFALNLLSILTLILVILTILGIAFASLIVKCLYPGLAPETTVLAIKLTR
                     IIFPYLFFIGLSSTFIAILNSHNYFFMTGLSSALLNVGMISTIIIPYYVLKVSGEELI
                     IWAGWGVIVGGFLQTIVNFPYLKKIGYRWAFCFRFGSTALAQLWKRIIPSMIGIGIRE
                     INLIADSLMASFLPIGSITALSFGNRLMQLPLGIFGISTGTAVLPFYSRCVTNGKYDE
                     LSESIRFTGLNLAYIMLPVTTIILALGEDFVRILFQSGAFNERAVWMTSQALIFYSLG
                     LVFFGLNQTITPIFYAHKDTKTPVKIAAAMVALNITLNFILMQFMAHRGLALSTSITA
                     GVNYFILLYLIRKKMPGITFSGIIRNIAKTLLICLIIFFLLVIGRNILPVQGRIELLI
                     RDAVFSVVAFGLFYLSAILIKIDYITQAGKALCKRFLRK"
     gene            1446..3260
                     /gene="uvrC"
                     /locus_tag="PKM80_02285"
     CDS             1446..3260
                     /gene="uvrC"
                     /locus_tag="PKM80_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNV92764.1"
                     /translation="MQALSKEIESKLSLLPDQPGVYLWKDKNGTIIYIGKALNLKHRV
                     RSYFASSGKDVKTEHLVKNIADLEYIITNSESDAFILEATLIKHHRPKYNIMLKDDKR
                     YPFVKITVNEPFPRIFITRELLKDDAKYFGPYTEVRYLRSTLRDLEWIFPLRNCNRNI
                     PKTKYKQPCMNYQLGKCPAPCVGCINQEDYAVIVNRLLRFFSGRYTELIDELRAEMQN
                     ASDSLDFEEAAKLRDRIVAIQSIQKRQSVFNVEGGNTDIIGFYREEKRAVCVVFKMQN
                     GAIIHQENYPLANLDYEESDSILASFLQLYYADKEEMPDEVLLPFAPKDIDKLNSWLG
                     NKLQVPQRGEKTKLIAMAKRNAFNIVEESKLAHLRKANRTIFPIQELKEVLKLPKLPR
                     KIVCMDISTIQGSDTVSSAVFFENGKSKKKYYRHFIIRSIDTQNDFAAMQETMARFLT
                     ETEKDEQMKPDLIIVDGGKGQLSASEQILNSSGKNDISLIALAKRAEEVYLSGNEQSI
                     VLPRSSSALRLLITIRDEAHRFAITFHRKRRTQRTLESALEAIPGIAETNKFLLLKEL
                     GSIDNIAKADIETLSSIKGIGIKTATKIYNHFHNEENK"
     gene            3257..4402
                     /locus_tag="PKM80_02290"
     CDS             3257..4402
                     /locus_tag="PKM80_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNV92765.1"
                     /translation="MKYRKMPYLDDKLSTLGFGCMRLPKNKEGKIDEEQALAMLHYAY
                     ENGVNYYDTAWSYHNGESEPLLGKFLSQIERNKVFIATKLPCWLVKNRADMDNFLEKQ
                     MEHLQTSYIDYYLLHSLNQKSWMEMKRLGVIDFLDKAKMEGKVRHLGFSFHDSYPAFK
                     KILLAYQWDFCQFLLNYLDTNYQAGMNGYKLAGQNGTGIVAMEPLRGGKLAGHLPENV
                     AAVWKKHNIRLSPVKFALRWVWNLEGCQVLLSGMSSLEQVKENVHYANICKANVLDAK
                     TLKIYKEVRREFLKRIPVMCSECRYCLPCPQKVAIPDIFGIYNDSIMFNEKDRHQKEY
                     ELFIPDESKADKCIACGACVSKCPRHINIPEEVKKIAEYFAECKVID"
     gene            4490..6586
                     /locus_tag="PKM80_02295"
     CDS             4490..6586
                     /locus_tag="PKM80_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNV92766.1"
                     /translation="MNSKYILLCIVTAIVIVGIYQIVAVGRVNYPEYNLKEGQVADVE
                     IIAPFDYPILKSTSELADEREQVLSQVNKPYEISDDPMFEAIKALDNIYAVLCSLQPT
                     LDPNAIATELKRNGYIFSSEGLSFATHPMLRDKIYESLRNKLRAIYKQGIYETLKEDS
                     VLIDQNGKLKTVNIREFLSIEQVKEDLVTNFPEAKTFVLEFSEQFLKPNLQVDEEKLN
                     ELNQDSLGTIYQSKGMVLRNEVIVRKNSRITQDEVRKLESLQEAYRRQKIRKSPLQEM
                     FLTLGLLMFVFLNIFLTNHYFGVQSKEEKIPVADFIPVNLGFILLVVFTVLSNYALSA
                     NNLIIPFSMIVIAGAILVNFEFGLLYSICNILVISPFLNWETFTPVIMILSSVICLLI
                     LRRQNAWHEYLMIWLYLLLSQIVVIISIGIYKRDTLLTILRSTGYSFISSTLSVSGIL
                     LIVPYYEHKWNRATKQTLLELLDFNHPLLKRLATEAVGTYHHSLVVGSLAEHSAEAIG
                     ANPLLARVGSYYHDIGKITNPDIFTENNEDSADIHSQMEPEESTQLIRNHVTDGIALA
                     KKYKIPQPVIDIIMQHHGNSVIRYFYEKASKEKKELSKDTYRYPGPRPQTKEAALVML
                     ADIVESTTKAKNIENEAEVAKIIDDTIHYLLKEGQFDEAPISMKDLQTVKQCFIPVLT
                     SIYRKRLDYPEPPKDE"
     gene            6579..7043
                     /gene="ybeY"
                     /locus_tag="PKM80_02300"
     CDS             6579..7043
                     /gene="ybeY"
                     /locus_tag="PKM80_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNV92767.1"
                     /translation="MNSLEVLGAVIIPESEQEKIAELICSKEDPDHCYQICVKIVDSN
                     TMKRYNKRYRGLAETTDILSFVTAELPFCESDLTFTEDGFPDKPIRFCDIIIDINQLF
                     YQRDSNIIEDNYRTVLIHGLLHLIGYDHIRSADAEKMRAKEEYYFKLTQGEN"
     gene            7046..8311
                     /locus_tag="PKM80_02305"
     CDS             7046..8311
                     /locus_tag="PKM80_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV92768.1"
                     /translation="MDDGKAFIIIVLFALSAFFSGSETALFSLSRVYLKRLENSKSKS
                     AKRILKLLKKPRELLITLLLGNTFVNLAISSFGTLLALQIGRKYSYTESSVVTIQIIL
                     TTILILFFGEISPKLIALSKADTLSGILSLPLLMFRYLFYPIVWVLVKMSELISSHQN
                     VDRYVGTKFTDEEFHNLIQSESSSHSLEEHEKKMLVGLFRFREAEIKEIYVPRVKITA
                     IEENQSIEDLKKIITESGYSRIPVYRGSIDEIVGIIYVKDLILYPEKKTIKQLMRPVW
                     FVTENMKVQTLLNQFKQRKLQVAIVVDEYGGTSGIISLEDILEEIVGEIRDEYDKEEI
                     PELIKIDENTYILSGNFSIRQFNENFSTDISLEDYDNLAEFLLAYYNHVPNIGDTVFY
                     DERIEFTILDADEKSIKQIQVKIKTGELT"
BASE COUNT         2777 a         1360 c         1550 g         2713 t
ORIGIN      
        1 gcttttttct ttggtactac ctatttaaat gatgctttta atgttggtta taatatacct
       61 aatttgcttc gacgactttt tggagaggga gctctttcta cagctttcgt acctatctat
      121 aatgagattt taattgaaga tggaaaagat aaacagatag attttgcttt aaacttgctt
      181 agtattttaa ctcttatcct tgtaattcta actatcttag ggatagcttt tgcctcttta
      241 attgtaaaat gtctttatcc tggactggct cctgaaacta ctgttctggc tataaagcta
      301 acccggataa ttttccccta tttgtttttc attggtttat cctctacttt cattgccatt
      361 ctcaattctc ataactattt ctttatgaca ggtctttcat ccgctttact taatgttgga
      421 atgatatcta ctatcattat tccttattat gttctaaaag tgagtggaga ggagttaatt
      481 atctgggctg gctggggagt gatagtagga ggttttttac aaacaatcgt caattttcct
      541 tacttaaaaa aaataggata tcgttgggca ttttgtttca gatttggttc tacagctctt
      601 gctcagcttt ggaaacgcat aattcccagt atgataggaa taggtattcg ggaaataaat
      661 ctgatagccg attcacttat ggcttctttt ttacccatcg gatcaattac agcattaagt
      721 tttggtaacc gtttaatgca attgccgtta ggtatatttg ggatttctac tggaacagca
      781 gttttacctt tttattctcg ctgcgtaact aatgggaaat atgatgagct atcggagagt
      841 attcgtttta caggtttaaa tcttgcttat attatgttac ctgttacaac tattatatta
      901 gcattaggcg aagattttgt aagaattctt tttcagagtg gtgctttcaa tgaaagagct
      961 gtttggatga cctcccaagc acttattttt tattctctgg gtttagtctt ttttggtttg
     1021 aatcaaacaa ttacgccaat tttttatgcc cataaagata ctaaaacccc agtaaaaatt
     1081 gctgcagcta tggttgcttt aaatataact ttaaacttca tccttatgca atttatggct
     1141 catcgcggtt tagctctttc cacatctatt actgcgggtg tgaattattt tattttgctc
     1201 tatctgatta gaaagaaaat gccaggtata actttttctg gaatcatcag aaacatagct
     1261 aaaactttac tgatttgcct tattatattc tttttattgg taataggacg caatatactt
     1321 ccggttcagg gtagaattga attattaatc agagatgccg ttttttccgt agtcgccttt
     1381 gggttgtttt atttatccgc tattttaatt aaaattgatt atatcactca ggcaggaaaa
     1441 gcactatgca agcgctttct aaggaaatag aatctaaact ttctttgcta ccagatcaac
     1501 ctggagttta tctttggaaa gataaaaacg gtactataat ctatataggc aaagctctta
     1561 atctaaaaca ccgggtaaga agttattttg cttcaagcgg caaggatgta aaaactgagc
     1621 atctggtgaa aaacattgct gatcttgaat atatcatcac taacagtgaa tcagatgcct
     1681 ttattttaga agcaactctt atcaagcatc accgtcccaa atacaatata atgctgaagg
     1741 acgataaacg ctatcccttt gtaaaaataa cagttaatga acctttccca cgcattttta
     1801 ttactcgtga attattgaaa gatgatgcca aatattttgg accatatact gaagtccgtt
     1861 atctacgctc aactttacgc gatctggaat ggatatttcc tttaagaaac tgtaatcgaa
     1921 atatccctaa aacaaaatac aaacaaccat gtatgaatta tcagctggga aagtgtcctg
     1981 ctccttgtgt aggatgcatt aaccaagaag attatgcggt tattgtaaat cgcttacttc
     2041 gttttttcag tggacgctat acagaattga ttgatgaatt gcgcgctgaa atgcaaaatg
     2101 cgtcggattc tttagatttt gaagaagcgg caaaattacg ggatcgaatc gtggcaattc
     2161 aaagtattca aaagcgccaa tctgttttta atgttgaagg tggaaataca gatattattg
     2221 gtttttacag ggaagaaaaa agagctgttt gtgtagtttt taagatgcag aatggagcaa
     2281 tcatccatca ggaaaattat ccccttgcaa atctggatta tgaagaatca gacagcatcc
     2341 tggcaagttt tttacagctt tactatgcag ataaagaaga aatgcccgat gaagttcttc
     2401 ttccttttgc tcccaaagat attgataaac ttaattcttg gctgggaaac aaattgcagg
     2461 ttcctcaaag aggcgaaaaa accaaactta ttgctatggc taaacgcaat gcttttaaca
     2521 ttgtagaaga aagtaaattg gcacatttgc gtaaagccaa ccgtactatt ttcccgattc
     2581 aggaattgaa agaagtctta aaacttccca agctgccccg taaaattgtc tgtatggata
     2641 tatctactat tcaaggaagt gatactgttt catccgcggt tttctttgaa aacggtaaaa
     2701 gcaagaaaaa atactaccgt cacttcatta ttcggagtat agacacccaa aatgattttg
     2761 cagcaatgca agaaacgatg gctcgttttt taacagaaac tgaaaaagat gaacaaatga
     2821 aacctgatct gattatcgtt gatggaggaa aaggtcagct ttctgccagt gaacaaattc
     2881 ttaactcttc cggtaaaaat gatatcagct taatcgcttt agcgaaaaga gctgaagagg
     2941 tctatctctc tggaaatgaa caatctatag ttcttccacg atcttcttca gcactacgat
     3001 tattaattac tattcgtgat gaagcacatc gttttgctat aacttttcac agaaaaagaa
     3061 gaactcaaag aactctggaa agtgctttgg aagctattcc tggtattgca gaaacaaata
     3121 aattcctgct actaaaagaa cttggtagca tagataatat agccaaagca gatatagaaa
     3181 ccctttcaag cattaaagga ataggaatta aaactgctac caaaatatat aatcactttc
     3241 acaatgagga aaacaaatga aataccgtaa aatgccttat ttggatgata aactatccac
     3301 tcttggtttt ggctgcatgc gcttgccgaa aaataaagaa ggaaaaatag acgaagaaca
     3361 agcattggct atgttacatt atgcttatga aaatggtgta aattattatg ataccgcttg
     3421 gtcttatcat aatggagaaa gtgaaccctt attgggcaaa tttcttagcc agattgaacg
     3481 caataaagtg tttatagcca caaaactacc ctgttggtta gttaaaaata gagctgatat
     3541 ggataacttt ctggaaaaac agatggaaca tttacaaacc agctatatag attactattt
     3601 gctacattca ttaaatcaaa agtcctggat ggaaatgaaa agattggggg ttattgattt
     3661 tctagataaa gcaaaaatgg aaggaaaagt aagacatctc ggtttttcct ttcacgatag
     3721 ctatcctgca ttcaaaaaaa tcctgcttgc ttatcaatgg gatttttgcc aatttttact
     3781 aaactatctg gatacaaatt accaagctgg aatgaatggc tataaattgg caggacaaaa
     3841 tggaactgga attgttgcta tggaacctct tagaggagga aaacttgcag gacatcttcc
     3901 agaaaatgta gcagctgttt ggaaaaaaca taatatccgt ttaagccctg taaaatttgc
     3961 tttaagatgg gtttggaatt tggagggctg tcaggttcta ctaagtggaa tgagttcttt
     4021 agaacaggta aaagaaaatg tgcattatgc gaatatttgt aaagcaaatg ttttagatgc
     4081 taagacacta aaaatatata aagaagttag gcgagaattt ctgaaacgaa tacctgttat
     4141 gtgttctgaa tgcagatact gtcttccctg ccctcaaaaa gtagcaatcc cagatatttt
     4201 tggtatatat aacgattcaa ttatgtttaa tgaaaaagac aggcatcaaa aagaatatga
     4261 acttttcatt ccagacgaaa gcaaagcaga taagtgtatt gcttgtggag cttgtgtttc
     4321 caagtgcccc agacatatta atatcccgga agaagtgaaa aagatagctg agtattttgc
     4381 tgaatgcaag gttattgatt aattacgggt gtattatgaa tagagaggat gttgaactta
     4441 tgttaaatga cacttgacaa agaacaagct ctataaaaaa gataaaaata tgaattcgaa
     4501 atacattctt ctttgcatag taacggctat agtaattgtt ggtatttacc agatagttgc
     4561 cgttggcaga gtaaactacc ctgaatataa tctgaaggaa ggtcaggtag cagatgtgga
     4621 gataattgct ccctttgatt atcctatttt aaaatctacc agcgaattag ccgatgaaag
     4681 ggaacaagtt ttatctcagg ttaataaacc ttatgaaata tctgatgacc cgatgtttga
     4741 ggctatcaag gcattagata atatttacgc tgttttatgt tctttacaac caactttaga
     4801 tccaaatgct attgccacag agctgaaacg aaatggatat attttttctt ctgaagggct
     4861 cagttttgct acacatccta tgcttcgtga taaaatatat gaatctctgc gtaataaact
     4921 tagagctatt tacaaacaag gcatctatga gacattgaaa gaagatagtg tattaattga
     4981 ccaaaatgga aagctgaaaa cagtaaatat aagagaattt ctctctatag aacaggttaa
     5041 agaagatctg gtaacgaatt ttccggaagc taaaaccttc gttttggaat tttctgagca
     5101 attcctgaaa cctaacctcc aagtagatga agaaaaacta aatgaactga atcaagattc
     5161 tctgggaaca atttaccaaa gtaaaggtat ggttttacgg aatgaggtta tagttcgcaa
     5221 aaactccaga ataactcaag acgaagttcg taagctggaa tcgttacagg aagcatatcg
     5281 gcgtcaaaaa atacgaaaat ctccccttca agaaatgttt cttactttgg gtttgctgat
     5341 gtttgtcttt ttaaatattt ttcttacaaa tcactatttt ggtgttcaaa gcaaagaaga
     5401 aaagatacca gtggcagatt ttatacctgt aaacttgggg tttatcctct tagtggtttt
     5461 tactgtgctt agtaattatg ctctttctgc aaacaattta ataattccct tttctatgat
     5521 tgttatcgca ggagccattc tggttaattt tgaatttggg ttattgtata gcatctgcaa
     5581 tatactggtt atcagtcctt ttctgaattg ggaaacattt actccggtca ttatgatctt
     5641 atcctcggta atatgtttat taattttacg ccgtcagaat gcctggcacg aatatttgat
     5701 gatctggtta tatttgcttt tatcccaaat agtggtaatt atcagtatcg ggatttataa
     5761 aagagatact ttgctaacaa tactgcgttc aacaggttat agctttatat cctcaactct
     5821 tagcgttagt ggaattttgt taattgttcc atattatgaa cataaatgga atcgggcaac
     5881 aaaacagaca ctattggagc tcctagattt taatcatccc cttctaaaaa gattagcaac
     5941 tgaagcagtt ggaacatatc atcattcttt ggtagttgga agcttggcag aacattctgc
     6001 tgaagctatt ggagctaatc cactattagc cagagtaggc agttattatc atgatatagg
     6061 taaaattacg aatccagata tttttacgga aaacaacgag gattcggcag atattcattc
     6121 gcaaatggaa ccagaagaaa gcactcaatt aattcgtaat catgtaacag acgggatagc
     6181 tttggcgaaa aagtacaaaa tcccgcaacc ggtgatagat attattatgc agcatcatgg
     6241 caattctgtt attcgctatt tttatgagaa agctagtaaa gagaaaaaag aattgtctaa
     6301 agatacctat cgttatcctg gacctcgtcc tcaaaccaaa gaagcagctt tagtaatgct
     6361 tgctgacatt gtagaatcta ctactaaagc caaaaatatt gaaaatgaag cagaagttgc
     6421 taaaattatt gatgatacaa tacactatct attgaaggaa ggtcaatttg atgaggcacc
     6481 tatttctatg aaagatctgc aaaccgtaaa acagtgtttt attccagttt taacaagtat
     6541 ctaccgtaaa cgtcttgatt atccggaacc tcctaaagat gaatagttta gaagttttag
     6601 gtgctgttat tattcccgaa tcggaacagg aaaaaatcgc agaacttatt tgttctaaag
     6661 aagatcctga tcactgttat caaatctgtg ttaagatagt agatagtaac acaatgaaac
     6721 gctataacaa acgttatcgt ggtttagcag aaactaccga tattttaagt tttgtaactg
     6781 cagaattacc tttttgcgag agtgatctta cttttacaga agatggtttc cccgataagc
     6841 cgatacgctt ttgcgatatt attattgaca taaatcagct tttttatcaa agggattcta
     6901 atattatcga agataattac cgcacggttt tgattcatgg cttattgcat ttaattggtt
     6961 atgatcatat tcgcagtgct gacgcagaaa aaatgagagc taaagaagaa tattatttta
     7021 aacttacaca aggtgaaaat tagaagtgga tgacggtaag gcatttataa ttatcgtcct
     7081 ttttgcgtta tcagcttttt tttccggtag cgaaacagct ttattttcgc tttccagggt
     7141 ttatcttaaa cgcttggaaa acagtaaaag caaaagtgca aaaagaatcc ttaagttact
     7201 aaaaaaacca agagaattgc taattacctt gcttttaggc aatacttttg ttaatttggc
     7261 tatatcctct tttgggacac tactggcttt acaaattggc aggaaatata gctatacaga
     7321 atcgtccgtg gttactattc aaattattct cacgaccatc ctgattttgt tttttggaga
     7381 aatttccccg aaattgattg ctctatccaa agctgatact ttaagtggta tactttcttt
     7441 accattgctt atgttccgct atcttttcta tcctatcgtt tgggtgctag taaaaatgag
     7501 tgagttgata tcttctcacc aaaatgtaga ccgctatgta ggaactaaat ttactgatga
     7561 agaatttcac aatcttattc aatctgaaag cagcagtcac agtttagaag aacacgaaaa
     7621 gaaaatgctt gttggcttat tcagattcag agaagcagaa attaaggaaa tatatgtccc
     7681 ccgtgttaaa attaccgcta ttgaagaaaa ccaaagtata gaagacctaa aaaagataat
     7741 cacagaaagc ggttattctc gaatccctgt ttatagaggt agtatagatg aaattgttgg
     7801 gataatatat gtaaaagact tgattctcta tccggaaaag aaaaccataa agcaattgat
     7861 gcgtccagtt tggttcgtta cagaaaatat gaaagtgcaa accctgttaa atcaattcaa
     7921 acagagaaaa ttgcaggtag ccattgtagt tgatgaatat ggaggtacaa gcgggataat
     7981 ttctttggaa gacattctgg aagaaatagt tggtgaaatt agagatgagt atgataagga
     8041 agagattccc gaattgatta aaatagatga aaacacttac attttgagcg gaaactttag
     8101 catccgtcaa ttcaatgaga atttctctac tgatatttct ctggaggact atgataatct
     8161 tgctgaattt ctattggcat actataatca tgttcccaat attggtgata ctgtatttta
     8221 cgatgagaga attgaattta ctattttaga tgctgatgag aagagcataa aacagattca
     8281 agttaaaatc aaaaccggag aattgacctg aaatatatcc taacactatt aatgcttgtt
     8341 aatggatttt tagcggccat aaccacagat tattatatga aagctgttgg gattaaaatt
//
LOCUS       DAORHB010000043         8310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_8130_length_8310_cov_3.899818, whole genome shotgun sequence.
ACCESSION   DAORHB010000043 DAORHB010000000
VERSION     DAORHB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8130_length_8310_cov_3.899818"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1370)
                     /locus_tag="PKM80_02310"
     CDS             complement(153..1370)
                     /locus_tag="PKM80_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV92769.1"
                     /translation="MSIIGIIRIAFKSLARNKTRTFLTMLGIIIGVAAVITMLAIGQG
                     AKKIVEDQISSMGTNVLMVTVNFTRSTTNVRQAAGSGTLLELDDVFAIRSQVEGALYA
                     SPVYNSFAQLKFESQNWRTGIMGVDTDYFYIRNMNTEKGELFYDSDVENGTKVCVIGK
                     TVADNLFGDIDPVGQIIRIRNIPFTVKGVMESKGQNVMGGDQDDSVWAPYTTVYARLL
                     GRRWRNMMIVVSAVSKDRIPEVQQDILELLQNRHRGTTSEEFLVRSQTDIAETASKVS
                     DTMTILLASIAGISLLVGGIGIMNIMLVSVTERIKEIGIRMAVGAGKRDVLLQFIIEA
                     VAISILGGFIGILLGFGASAIVGNAMDWSIAVTPFSVLISVGFSMAIGIFFGWYPASK
                     AANLNLIDALRYE"
     gene            complement(1367..2119)
                     /locus_tag="PKM80_02315"
     CDS             complement(1367..2119)
                     /locus_tag="PKM80_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92770.1"
                     /translation="MSDLLIRTENLVKTYQMGEIQVHALRGVSLEINKGEFVAIMGAS
                     GSGKTTLMNLLGCLDLPTSGEYYINNIGIHEITPTELTKIRNQMIGYVFQNFYLLPRT
                     TALENVELPLLYRNKMSHHQRIRKAIEALTTVNIANRAKHFPNQLSGGQQQRVAIARA
                     IVNDPMFLLADEPTGNLDTRTSLEVMTIFQKLHKEGKTILLVTHENDIARYAQRLITF
                     RDGRIISDVPNKNPQNAEYDLAHLPKLPGDEE"
     gene            complement(2121..3413)
                     /locus_tag="PKM80_02320"
     CDS             complement(2121..3413)
                     /locus_tag="PKM80_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV92771.1"
                     /translation="MKKYLKYIIIIALIIIAIIVISSMRKAKNKPEFRTVTPSFGNIK
                     EVVTATGSLNPYVLVSVGTEVSGKIDKLYKDFNDRVKKGELLAKLDTDILSTNLEAAK
                     ADLAKAKTALDQAKLDYDLQKELLEKQMTPEYDAKKALYAYQTAQQNYNNAQLTLQRA
                     QKNLANAYITSPIDGIVVSRDVDAGQTVAASMSSPTLFQIANNLDQMQISANVDEADI
                     GKIIVGLPVEFTVDAYPSENFSGTVKQIRLNPTTESNVVSYSVIIDAANPEQKLLPGM
                     TTNVTIIIQSRENVMRISEAATRFKPSKEVWEQFGLKWSDDLIKQAQKEAYASLASAM
                     SSSSNTDSIKVSKNTSGKRTQKGSANNVSSGKLALVWVLENDVPKAKGIKTGISESGF
                     IEVISGLNGNEVLITGVNSKKNSDTTNGTQGGPGMRPF"
     gene            complement(3449..4693)
                     /locus_tag="PKM80_02325"
     CDS             complement(3449..4693)
                     /locus_tag="PKM80_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV92772.1"
                     /translation="MKVITVILMLLPLLLAAQSYTLEELISFGLENSFSVQKSSLSYQ
                     SSRSMLNTSKWNLLPDASVTGGVNKDFDPAAGTDDVSSSAGLSISKTISLNDAAYFNY
                     LYASLDTKTANMQLEQTRRTYAYNVFSAYIKVLSSQKQRSSLEENLLIQTRVWEQSKI
                     LLQLGKITPFEVKQNEIEVLNSNIALMQLDNTIANARKELFALVQMQDEGYNLTELDL
                     DIQKELPAFNTENNADIQILNQELKRLNLQLKQNKLDDFPRLNLAYNFDRTVSGADFD
                     FDRYNTNHGISLSLSYPLLNLFENSESRTRTKISRQLSQVSIDEKKDQLQRNYDSALR
                     ELQYLKRMNELYSEQLAQSRDQITQAEERYRLGLIELLELDKTRTNYIETDISYNNNL
                     YSIMAKQEEINYLLSEPVLGKW"
     gene            complement(4900..5346)
                     /locus_tag="PKM80_02330"
     CDS             complement(4900..5346)
                     /locus_tag="PKM80_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92773.1"
                     /translation="MKVNFKYGLSGYTGKADDLVFCYDRISGKIYARKRVYPRLTEEN
                     ARIANISRNIFALQPSEVYKNDLRLYLLRYNGLKVNRYKQLRSWVNLYHLLMANMAKL
                     YPEIDLKTITREQIYQENLPCISVKKAVEAGVLPQVNGYERMEALI"
     gene            5776..6777
                     /gene="argF"
                     /locus_tag="PKM80_02335"
     CDS             5776..6777
                     /gene="argF"
                     /locus_tag="PKM80_02335"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNV92774.1"
                     /translation="MAFNLKNRNFLTLMDFTPKEIQFLLDLSMELKKAKYAGTEQQKL
                     KGKNIALIFEKDSTRTRCAFEVAALDQGAHTTYLGPTGSQLGKKESVADTARVLGRMY
                     DGIEYRGFGQEIVEELAQYAGVPIWNGLTDQDHPTQVLADFLTAMEHLNKPLNEMVFV
                     YCGDGRNNMANALMIGACKTGMDFRIVAPKSLFPDQNLVNKCLEVAKETGAKIAFTDN
                     IDEGVKNADIIYTDVWVSMGEPDSVWEQRITLLKPYQVNSEMMLKTGKSTTIFMHCLP
                     SFHDLKTKIGKDIYDKFGLTCMEVTDEVFEGPQSVVFDEAENRMHTIKAVMVATLGS"
     gene            complement(6847..>8310)
                     /locus_tag="PKM80_02340"
     CDS             complement(6847..>8310)
                     /locus_tag="PKM80_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV92775.1"
                     /translation="DSQTTIQNEISINNLTTGQLYHFALTAVDDNDNESMPIFNQAVP
                     RLIPSVPVSFTDHPLPQVVELVWQANTELDLAGYKLYRSLDSSEIGELIATLPPTVQS
                     FTDNNVQGGDFYYYYRLCAFDNDNNNSPYTAVIKTRPINMNYGVLIVDETMDFSGSNL
                     FQPTDEQVDSFYDQLVAGLWSTSHFDLATLTDTLRLCDLGIYSVIIWHGNDYTDMQYP
                     YYIKDALKSYIYYGGKVLFSLYHPSMAFEMNSGYPASFPSGSFINEVLGISNVDYSTS
                     ARFHKAVSTDPNYIDVNVDPAKVPPGLNGHLFHIEVINPNAQATPIYSYASAYDHNTL
                     QGVLNGGVVGILNNYYAGKTVTLSFPLYSMDFNQSRQVLRYIVETCFEQPISVSDEVI
                     NQVPEISILPNQPNPFSSTTQFNILCKNISAPLEVKIYNLKGQLVKTLYKGIADSKNN
                     ISWDGTDYKDKQTASGIYLIKANQGNTIETRKILKLK"
BASE COUNT         2524 a         1560 c         1439 g         2787 t
ORIGIN      
        1 tatgtaagag aagagtcatc attgccaaaa aagctgtaat gcttattaca aatttatttg
       61 acagtgatga ctcataattt gagcgactta ttgcttattt gatttgcatc gcagtgtttt
      121 ttcttcttgg gagtttatat tctcaaacat atttattcat atcgtaaagc atctatcaga
      181 ttcaggtttg ccgctttaga tgctggatac catccaaaga agatacctat agccatcgaa
      241 aaacctactg atattaacac ggaaaaagga gttacagcga tgctccaatc catagcattt
      301 ccaactattg ctgaagctcc aaaaccaaga agtattccaa taaaaccacc taaaatacta
      361 atagcaactg cttcaataat aaattgtaat aaaacatctc tttttcctgc gcctacagcc
      421 atcctgatcc caatttcttt tatacgctct gtaactgaaa ccagcattat attcataatt
      481 ccaattccac caacaagtaa tgatatacct gctatgcttg ctaaaagtat agtcattgta
      541 tctgatactt tactggcagt ttcagcaata tcagtttgac tcctaactaa gaattcttcg
      601 ctggttgttc cgcgatgtct gttttgtagt aattctaaaa tatcttgttg cacttcggga
      661 attctatctt tggaaacagc cgacacaaca atcatcatat ttctccatct acgacccaat
      721 aatcgagcat agacagttgt ataaggtgcc caaacactat catcctgatc tccacccatc
      781 acattttgtc ctttggattc catcactcct ttcactgtga aagggatatt gcgaatacga
      841 ataatctgtc caactgggtc tatatctcca aagagattat cagcaacagt tttcccgatt
      901 acacacactt tagttccgtt ttcaacatcg ctatcgtaaa acaattcacc cttttcagta
      961 ttcatattgc ggatgtagaa ataatctgta tctacaccca taatgcctgt tctccaattc
     1021 tgggattcaa atttcaattg ggcaaagcta ttataaacag gtgaagcata taaagcacct
     1081 tctacttgtg atcgaatagc aaaaacatca tcaagttcaa gtaatgtacc acttccagct
     1141 gcttgacgaa cattagtagt gcttcgtgta aaattaactg taaccataag tacatttgtg
     1201 cccatagaag atatttggtc ttctactatt tttttggcac cttgcccaat ggcaagcata
     1261 gttatcacag ctgccacacc gataataatt cccagcattg ttaaaaaagt tctggtttta
     1321 tttctagcaa gcgatttgaa agctatgcga attataccta ttatactcat tcttcatctc
     1381 caggtaattt aggaaggtgg gctaagtcat attcagcatt ttgaggattt ttattgggaa
     1441 catcagatat tatgcgtcca tcacgaaaag taatcaatct ttgtgcatac cgggcaatat
     1501 cattttcgtg ggtaaccaga aggattgtct ttccttcttt atgtaatttc tggaaaatag
     1561 tcataacttc caaacttgtt cttgtatcca aatttccagt tggctcatcg gcaagtaaaa
     1621 acataggatc attcacaata gcacgggcaa tagcaactct ttgttgttgc ccacctgaaa
     1681 gctgattagg aaaatgtttt gctctgttag caatattaac agtagttagt gcttcaattg
     1741 cttttctaat tctctggtga tgagacattt tatttctata tagcagaggg agttcaacat
     1801 tttccaaagc agttgttctg ggcagtaaat agaaattctg aaacacataa ccaatcatct
     1861 gattgcggat tttggttagt tcagtaggag tgatttcgtg gatgcctata ttatttatat
     1921 aatattcacc gctggtaggt aaatccagac agccaagcaa attcataaga gttgttttgc
     1981 cgcttccgct tgctcccata atggcaacaa attctccttt atttatttcc agagaaacac
     2041 ctcttaatgc gtgaacctgg atttctccca tctgatatgt tttaactaag ttttctgttc
     2101 taatcagtaa atctgacata ttagaaaggc ctcattccag gaccaccttg cgttccatta
     2161 gtggtatctg aattcttttt actattcact ccggttatca atacttcgtt accgttaaga
     2221 cctgaaataa cttcaatgaa accactttcg gaaattccag tttttattcc tttagctttt
     2281 ggaacatcat tttccagtac ccagacaagt gctaattttc cagaacttac attattagct
     2341 gatccttttt gagttctttt tccggatgta ttcttgctta ccttaattga atctgtatta
     2401 ctggaactgg acattgcaga agccagagaa gcataagctt ctttttgggc ttgtttgata
     2461 agatcatcac tccattttaa cccaaattgt tcccaaactt ctttactggg tttgaagcgt
     2521 gtagctgctt ctgaaattcg catcacattt tctcgggatt gaataataat agttacattt
     2581 gtagtcatac caggaagaag tttttgttca ggattggctg catcaataat gacgctatag
     2641 gatacgacat tggattccgt agttgggttt aagcgaattt gcttcacggt accggaaaaa
     2701 ttttcgctgg gataggcatc caccgtaaat tccacaggca aaccaactat tatttttcca
     2761 atatctgctt cgtccacatt agctgaaatt tgcatttgat cgaggttatt agctatttga
     2821 aataaagtag gtgaactcat actggctgct acagtttgtc ctgcatctac atcacgggaa
     2881 accacaattc catcaatagg actggtaata tatgcattag ccaagttttt ttgagctcgc
     2941 tgtaaagtta attgggcatt attataattc tgttgtgcag tttgataagc atagagggct
     3001 ttttttgcat catattcagg ggtcatttgt ttttccagaa gttctttttg caaatcataa
     3061 tccagttttg cttgatctaa agcagttttt gcttttgcca aatcagcttt agcagcttcc
     3121 agattagtag aaaggatatc cgtatccagt tttgctagta attctccttt ctttactcta
     3181 tcgttaaaat ctttgtatag tttatcgatt ttgccactaa cttcggttcc tacactaact
     3241 aaaacataag gatttagcga tcctgttgca gttacgactt cttttatatt accaaaggaa
     3301 ggggtaacag ttctaaattc aggtttgttt tttgccttgc gcatactgga tatcacaatg
     3361 atagcaataa ttatgagcgc aattataatg atatacttga gatatttttt cattagttgt
     3421 ccttgatttt tatcttataa attttacttt accattttcc taaaactggt tcagaaagca
     3481 gataatttat ctcttcctgt tttgccatta tagaatatag attgttatta taggagatat
     3541 cggtttcaat ataattggtt cgtgttttat ctagttccaa aagttcaatt agacctaaac
     3601 gatagcgttc ttctgcctgg gtaatttgat ctctggattg agccaattgt tcactgtaaa
     3661 gttcattcat tcttttcaaa tattgcaatt ctcgtaaagc actatcatag tttctttgta
     3721 actgatcctt tttttcatca atgcttactt gggaaagctg acgactgatt tttgttctag
     3781 ttctgctttc agaattttca aataaattca ggagaggata gcttagcgaa agagatattc
     3841 catgattagt gttgtaacgg tcaaaatcaa aatcagcacc acttactgta cggtcaaaat
     3901 tataagccaa attaagccgg ggaaagtcat ccagtttgtt ttgctttaat tgcaagttca
     3961 gtcgtttcaa ttcttgattt aaaatttgaa tatcggcatt attttctgta ttgaaagcag
     4021 gaagctcttt ttggatatcc agatccagct ctgttaaatt ataaccttca tcttgcattt
     4081 gtacaagggc aaaaagttcc tttcgtgcat tagcgattgt attatccaat tgcattaaag
     4141 ctatattgga atttaaaacc tctatctcat tctgcttaac ttcaaaagga gtgatttttc
     4201 ccaattgcaa tagtattttg ctttgttccc aaacccgggt ttgaataaga agattttctt
     4261 ccagagaact gcgttgtttt tgtgaactta gcacttttat atatgcgctg aatacattat
     4321 atgcataagt tctgcgagtt tgttccagct gcatatttgc tgttttcgta tccaaagaag
     4381 catatagata attaaaataa gctgcatcgt ttaaactgat agttttactt atggataaac
     4441 cagcagaact gcttacatca tcagttccag cggcaggatc aaaatcttta tttactcctc
     4501 cagtaacaga agcatccggt aataaattcc attttgatgt attcagcatt gatcttgagc
     4561 tttgataact tagagagctc ttttgaacac tgaaactatt ctccagtcca aaacttatca
     4621 gctcttccaa agtgtaactt tgggcagcta acaatagggg cagcaacatt agtataactg
     4681 tgataacttt catttttttc ttttttcctt gattccttac aatttcctta tctgtatttt
     4741 gtatctcgtt tcttttaatt ataccatctt atgcagtata tcctgcaact ttttttatat
     4801 ctacagacaa agatttcttt tttaccttcc aatatttctc tctttactcg tcaatagttt
     4861 tcttccagac tgcaaaaacg aagaggataa ctttttcctt tatatcaatg cttccattct
     4921 ttcatagcca ttcacttggg gaagaacacc tgcttcaacg gcttttttta cacttataca
     4981 gggtaaattc tcttgatata tttgttctct ggttatcgtt ttcaggtcta tttccggata
     5041 gagtttagcc atatttgcca ttagcaggtg gtacaaattt acccaagagc gtagctgttt
     5101 atatctattt acttttaatc cattgtaacg caaaagataa agccgcagat cattcttgta
     5161 tacctctgaa ggctgtaggg caaatatgtt gcgagatata ttagcaattc gagcattttc
     5221 ttcggttaag cgaggatata ctctcttcct tgcgtaaatt ttcccggata ttctgtcata
     5281 acagaacacc aaatcatcag ctttaccggt gtatcctgac aggccatatt tgaaatttac
     5341 tttcattttg ttttctccta tttcgtcctt ctatatatat agggggtgac aaaagtgggg
     5401 aagtggtgac aaaaatcgtg tttttttgcc atcaaataaa aagttctttc gaacatacat
     5461 accggactta cacagccgat atgattccca tatccttccc atatcggata agggaatcat
     5521 atgggagtgt ttttgctggt atattggcta tgattaagtt tccatactaa atactcttag
     5581 gagaattgat taactaaccc tgggagaaga cagttcataa agccccataa ccccaagaaa
     5641 agagaaactc attgacagtt aagccatact tttaaaaaag gataacactc gcttagaatg
     5701 aaaaaccgtt tttttttact taaagagtct ttagcgataa aaccaaatac taatataaca
     5761 taaaaggaga ccaaaatggc tttcaacctt aaaaaccgta actttcttac ccttatggat
     5821 tttactccta aggaaattca atttctgctt gatctgtcaa tggagcttaa aaaagccaaa
     5881 tatgcaggaa ctgaacagca aaaattgaaa ggaaaaaaca ttgctcttat ctttgaaaaa
     5941 gatagcacac gcactcgttg tgcctttgaa gttgccgctt tagatcaagg tgctcataca
     6001 acatatttag gcccaactgg ttcacaattg ggcaaaaaag aatcagttgc cgatactgca
     6061 agggttttag gcagaatgta tgacggaatt gaataccgtg gcttcggaca agaaattgtg
     6121 gaagaattag cccaatatgc cggtgttcca atttggaatg gtttaaccga tcaggatcat
     6181 cctacccagg ttttggctga ttttttaaca gctatggaac atttaaacaa gcccttgaat
     6241 gaaatggttt tcgtttactg cggagatgga cgcaataata tggcaaatgc tttgatgatt
     6301 ggtgcttgca aaactggtat ggattttcgc attgttgctc ctaaatctct attcccagat
     6361 caaaatctgg ttaataaatg cctggaagtt gccaaagaaa ccggtgccaa aatcgctttt
     6421 actgataata ttgacgaagg tgtgaaaaac gctgatatca tttatactga tgtgtgggtc
     6481 tctatgggtg aaccggatag cgtttgggaa caaagaatta ctcttcttaa gccctatcag
     6541 gtaaacagcg aaatgatgct taaaactggt aaaagtacaa ctatatttat gcattgttta
     6601 ccttccttcc acgatctgaa aaccaaaatt ggcaaagata tttacgacaa attcggctta
     6661 acctgtatgg aagttacaga tgaggtcttt gagggacccc aatctgtggt ctttgatgaa
     6721 gctgaaaaca gaatgcatac aattaaagca gttatggtag ccactttagg atcgtaaaat
     6781 aaatctaaat ataaatatgg gagcgggata acataatacc tgctcccatt ttcgtaataa
     6841 tactgtttat ttcaatttca agattttacg ggtttctatt gtattgccct gatttgcctt
     6901 aattagatat attcccgagg cagtttgttt atctttatag tctgttccgt cccaggaaat
     6961 gttatttttg gaatcagcaa tgcccttgta aagagtttta actagctgac ctttcagatt
     7021 gtaaatctta acttccagtg gggcactgat gtttttacag aggatattaa actgcgttgt
     7081 actagaaaaa gggttgggct gattgggtaa aatagaaatt tcggggacct gatttataac
     7141 ttcatcggaa actgatattg gttgttcaaa acaggtctct acgatatagc gcaaaacctg
     7201 acggctttgg ttgaaatcca ttgagtataa gggaaaactt aaagttactg ttttacctgc
     7261 ataataattg tttaggattc caactactcc tccatttaaa acaccttgca gggtattatg
     7321 atcataagcg gaagcataac tataaatcgg agtagcttga gcattcggat tgataacttc
     7381 aatatgaaat aaatgaccat tgagaccagg tggaacttta gcaggatcaa catttacatc
     7441 aatataattg ggatcagtac ttacagcttt atggaaacgc gcacttgtac tatagtctac
     7501 attgcttatt cctagaactt catttatgaa agaaccagaa ggaaaagagg caggataacc
     7561 actattcatt tcaaaagcca tactgggatg ataaagagag aacagaactt ttccaccata
     7621 ataaatatag cttttaagag catccttaat gtaataggga tactgcatat cggtataatc
     7681 gttgccatgc cagataatta cggaataaat acccagatca cacagacgca aggtatcagt
     7741 taaagttgct aaatcgaaat gagatgtgct ccataaacca gcaaccaatt gatcataaaa
     7801 gctatcaacc tgttcatccg ttggttgaaa aagattgcta ccgctaaaat ccattgtttc
     7861 atcaacaatt aaaacaccgt aattcatatt tataggtctg gtttttatca cagcagtata
     7921 aggactgtta ttattatcat tatcaaaagc acagagtcga tagtaataat aaaaatctcc
     7981 tccttgaaca ttgttatccg taaaactttg aacagtggga ggtaaagtgg caataagctc
     8041 accgatttct gagctgtcca aagaacggta taatttataa cctgctaaat caagctccgt
     8101 atttgcttgc caaacaagtt caactacctg aggcaaagga tgatctgtaa aactaactgg
     8161 gacggaagga attaatctgg gaacagcttg attaaatata ggcatacttt cattatcatt
     8221 atcatcaacg gcagttaaag caaagtgata aagctggccg gttgtcagat tgttaatact
     8281 tatctcattc tgaattgtag tttgagaatc
//
LOCUS       DAORHB010000044         8219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_8266_length_8219_cov_5.365630, whole genome shotgun sequence.
ACCESSION   DAORHB010000044 DAORHB010000000
VERSION     DAORHB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8266_length_8219_cov_5.365630"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            483..1334
                     /locus_tag="PKM80_02345"
     CDS             483..1334
                     /locus_tag="PKM80_02345"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV92776.1"
                     /translation="MPNEHKLDKAIRYIEAGLKTRLILEDIAEQANMSAWHFHRVFLA
                     STGITVGEYIRKRRMSEAAKELVFTEKPIKQLASEYQYESQAAFTRSFMGVYRITPGK
                     LRRQLGPLQYFNALLPQTKGEKMLTPQIKHKDAFRVVGIACQNTMKNNNIPSLWNEFD
                     IMICDKITECLNPGTAFGICFYQDQSVVNEDTPFTYLAGVEFPLEKAVPEGLEYRDIS
                     DADYAVFEHCGSLDTLSQTYSFIYKEWLPNSGFQRKKSDDFELYDHRFQFGTEKSIME
                     IWVPIEK"
     gene            complement(1565..2560)
                     /locus_tag="PKM80_02350"
     CDS             complement(1565..2560)
                     /locus_tag="PKM80_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNV92777.1"
                     /translation="MLKKTLYLLALCLLICSNLSAGSKKPVWISDPYSQYPAHRYLCS
                     VGMANDLKSAKNDALKNIAGFFETRVTAVTKSMESESNSASGSQINSSITTSVKAISE
                     GTLKYTEIKATWQDPTTKTYYALAALDKTAVQALYEKEIVESEDYIANCLFKTDDPLI
                     RFAKLCQAIDKHNATQNALVYYNALAGESGSFLRPAFSMQELKAMRAETAKEIPIKIL
                     VVGDESAYLETALRNVIRQLGFSENGISENTEYELSATLETDPQKTIGKQIYQPLRIN
                     ISLKKGDNLLFSYVDKTEQGGPTIEEANSRSIKVLAKNIENKFNKEFTQYLLSIE"
     gene            complement(2651..3367)
                     /locus_tag="PKM80_02355"
     CDS             complement(2651..3367)
                     /locus_tag="PKM80_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92778.1"
                     /translation="MRLSRILLILAILLSMLSLHTALDAKKKKKEVEIKLYLDDAQLE
                     NPEYYNKLPVLSLESFATALLTPKTTNFKIINHNYQIARADSKIAEEMIAVSLGMRQE
                     FVKVEAEGKSLLSGNLEGSEAEISEAMKTKLEDLKNNKAQLSESQKVYFLSGFIFLTS
                     SIIREKIVLESTKMFVNEAKNAKGLDAVFYVKDIPYATNVVVGLPTLLTTQMKTISEF
                     ITIAKTQNIDIPEDVTASLF"
     gene            complement(3381..4499)
                     /locus_tag="PKM80_02360"
     CDS             complement(3381..4499)
                     /locus_tag="PKM80_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92779.1"
                     /translation="MRKLLTIILLSVTMLFSVAYLEAAVKVKVKATVETKKNKPKPEE
                     IKAAVNNAKILAVKKYASEKDTRKRELVNELMPDIEKNIDNYILEINYLNEGNKTNGI
                     WEQLLEVTIDDVQLELLINQKMKDVMEMAEPTYLSFVYVAREVAAVESQGQTIKKDLS
                     TKSDVSASGKIATSGDTDSNLSDPNKISTSSQAEVNIDKSISREGSMVYSQSVVNASE
                     GIYYRTFNPGEIDTKVTEIFNKANFEVVPAYEVGISPEKFAYDFAALNEISSSTQKEA
                     TDIAREAGLDFLAVAMLDVGREEIDPVSGGSKVYVKVNGYILDLRKKFAVKVCSAGPY
                     QFSGIGSNPSVAKTNALIEAAKNASQDLVDQLRVKLGN"
     gene            complement(4484..5593)
                     /locus_tag="PKM80_02365"
     CDS             complement(4484..5593)
                     /locus_tag="PKM80_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92780.1"
                     /translation="MVKNIGMRKNVKIAFIFTLAAGLLFANQDVKYAGFCFSGAYADI
                     SKNYPYTQKMLQDSSSAQSYLDKLFFDYFSANSELGNFTLDIHNQTKATLSLAIALNR
                     EEVAFEYYDNETKAIYNLGCTVFIMDFADLTILQSYPINVTYIDLYKGEPDQQTIIQT
                     IKKLYSNYILQKLMSNQKNIYIRSSGSLTIKVANVNFQEEALPFLGSYKTEPEIETYA
                     AIVAQHLTEALAFKMNLTVLPYSRSNLSYKMSLSFSDASIQNFTIPAASYDIDITVKR
                     FLKKLYKETAAERVDLYGVELEMRIYDAEFNQEYWKQTIQNGAAKQTVVGQIIGNEFY
                     KYNEILLLTLSDKIPATLKEDKKLMKGILKKCANY"
     gene            complement(5855..6694)
                     /locus_tag="PKM80_02370"
     CDS             complement(5855..6694)
                     /locus_tag="PKM80_02370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV92781.1"
                     /translation="MPIVAKVFEFEISEQDLERECAKVSITERKECLQNALKRLIDRY
                     LLLYKAIESGITISDAEYDKAIWELLDEENPLDLWSTSLDQLTPKEMENLLKERLMIN
                     KYINSFFSHDTPVTKDKVKEFYDEQKEYFLKAEQVRCSHILISNTKKDAKALAEEIRK
                     KIHNAEDFGYYCKEYSDCLSNSSCGDLGWFSRGIMIPEIEEVAFALKVGEISQPFPSK
                     YGYHILLKTGHSEKDYIPFEEIKDSLYARVQQIEKEYLLIRHLAELHKQYAAKIIIYK
                     EYL"
     gene            complement(6669..7613)
                     /gene="miaA"
                     /locus_tag="PKM80_02375"
     CDS             complement(6669..7613)
                     /gene="miaA"
                     /locus_tag="PKM80_02375"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNV92782.1"
                     /translation="MIPVITIEGATASGKSALAIALAESLCTEIISADSRQVYRYLDI
                     GTAKVAKAEQQRAKHHLLDIINPDETYNAGSFAADAEKIITSLHSEGKIPIICGGTGL
                     YIKALLNGLFTLPPLPTKIRYDLKQRLKEEGLTSLYSELLNIDPEFASKISENDTQRI
                     LRGLEVFSGTGIPLSEHWRKQKETGKYNAFRILIDMPRPQLYTRINERMEKMLAEGLL
                     QEIENLFTMGYTDHSPGLQCLGYKEFLPYFREEASIEDCAIIAAQHQRNYAKRQVTWY
                     RKYTFDLTIDSSSIILYNIVTKIKEGLKKKNANSSESI"
     gene            complement(7610..>8219)
                     /locus_tag="PKM80_02380"
     CDS             complement(7610..>8219)
                     /locus_tag="PKM80_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNV92783.1"
                     /translation="YDILLKNEEDYINPWQFHNTYIFIQVEDGLVIIDQHAAHERIIY
                     EKLLQRTGGAPAMRQKLIIPLVIDIPPYIASDIKELVEENLELLEKVGFTLKKFSGDS
                     IVIEEIPAELSDFASGQIFIEILKQLEQEMEIATDFKEGLAKSIACKAAIKANHKLTR
                     KEMLNLINDLFACKLPYFCPHGRPLILKIPVSDFEKKFKRIV"
BASE COUNT         2425 a         1455 c         1561 g         2778 t
ORIGIN      
        1 ttctgtcaag gaaaaaataa actaataagt attactctga tttacaagaa gttatggatg
       61 ctttataaaa tagggtcgtt ttgctgtttt gacttttggg caaagagaaa aaaagaacta
      121 atgatagaaa gcaataagaa gttacattat tataggttta ttagatacca tagggaacac
      181 cggaattact gaaagaagct aataaggcag atttgttttt ttaataatta tattagcaag
      241 gattccccaa gggtgaaaat aataataggt aggtatggct atcattatgc taagtatata
      301 ggtaagcgat acagtacagt aattatattc taacaattct ctttctggtt ttaggagcac
      361 agcaagtagt cggctagtta tgttatagta taattccgca aaaaaaatca aatctttctg
      421 cttgacttat gaaatgctga aaattatagc aataattatc aaaagagcgt taggagtgta
      481 aaatgcccaa tgagcataaa cttgacaaag caatacggta tattgaagct gggcttaaga
      541 caagactgat tttggaagac atcgctgaac aggctaatat gtcagcttgg cactttcacc
      601 gtgtattttt ggcttccaca ggaatcacgg taggtgaata tattcgcaaa cgcaggatga
      661 gtgaagctgc aaaagaacta gtttttacag aaaagcccat caagcaatta gcaagcgagt
      721 atcagtatga atctcaagca gcgtttacaa ggtcttttat gggggtttat agaatcactc
      781 ccggtaagtt aagacgccaa ttgggccctt tacaatattt taacgcgcta ttaccacaaa
      841 ccaaaggaga aaaaatgctt acacctcaaa ttaagcacaa agatgctttt cgcgtagttg
      901 ggatcgcttg tcaaaatact atgaagaata acaatattcc ttctttatgg aatgagtttg
      961 acataatgat ttgtgataag attacagagt gtttaaatcc tggaacagct tttggaatct
     1021 gcttctatca ggatcagagt gttgtgaatg aagatacacc ttttacttat cttgcgggag
     1081 tggaatttcc gttagaaaaa gcagttccag aaggattgga atatagagat atttccgatg
     1141 ccgattatgc tgtatttgag cattgtggtt cattggatac tctatctcaa acatacagtt
     1201 ttatttataa agaatggtta ccgaatagtg gtttccagcg caaaaaatct gatgactttg
     1261 aactatacga tcatcgcttt caatttggga cagaaaaatc tattatggaa atttgggtac
     1321 cgatagaaaa gtaataacaa gtcaaaacag atatttcaga ggtttgggag atatattaat
     1381 gtttcccaaa ccatttattt tttagtgttt acagaattgc tagttcatat gtcaagccac
     1441 aaaattatag ttgtagcttt tacgaggggc ttacgccacc gtcgctatcg ttaacagtgt
     1501 tttagcttct gggctataaa ggtataatta cattcaataa aagaccccta aaatgttctt
     1561 atctctattc tatgcttagc aagtactggg taaattcctt attgaattta ttttcgatat
     1621 tcttagccag tacttttatg ctacgcgagt ttgcttcttc aattgttggt ccaccctgtt
     1681 ctgttttatc cacataagag aaaagtaagt tatctccttt ctttaagctg atatttatac
     1741 gcaggggttg ataaatctgt tttcctattg ttttttgcgg atcggtttcc agggttgccg
     1801 ataattcata ttctgtattt tcggaaattc cgttttcgct aaaccccaat tggcgaataa
     1861 catttctgag ggcggtttcc agataggcag attcatctcc tacaacgagt attttaattg
     1921 gaatttcttt ggcagtttca gctcgcatgg ctttcaattc ttgcattgaa aaagcagggc
     1981 gcaaaaatga acctgattca ccagctaaag cattataata aactaaagca ttttgggttg
     2041 cattatgttt atctattgcc tgacacaatt tagcaaagcg gataagcggg tcatcggtct
     2101 taaaaaggca attggcaata tagtcctcac tttccacaat ttctttttcg taaagggctt
     2161 gcacggcggt tttatctaaa gccgcaaggg cataataagt tttagttgta ggatcttgcc
     2221 aagttgcttt tatttcggta tattttaaag ttccttcaga aatggctttt acggaggtcg
     2281 ttatagagct gtttatttga gagcctgaag cagaatttga ttcactttcc atacttttag
     2341 taacagctgt aaccctggtt tcaaaaaagc ctgcgatatt ctttaaagcg tcattttttg
     2401 cacttttgag gtcattagcc attcctactg agcaaagata acgatgggca ggatattggg
     2461 aatagggatc ggagatccaa accggttttt tacttcctgc agataggttg gaacagataa
     2521 gtagacacaa ggcaagcagg taaagagttt tttttagcat aataatatcc atatagcgaa
     2581 attacaatca gcaagggctg ctataaagct atgctgctta gaatcttttg ttataaagcc
     2641 aagggggtta ttagaacaag cttgcggtaa catcttccgg aatatcaatg ttttgggttt
     2701 tagcaatggt gataaattcg ctgatagttt tcatctgagt ggtaagcaaa gttggcagtc
     2761 caacaaccac atttgtggca taagggatat cttttacata aaaaacagca tccaaaccct
     2821 tagcattttt tgcttcgtta acaaacattt tagtgctttc caaaactatt ttttcgcgga
     2881 taatggagct agttagaaag atgaatccgc ttaaaaaata tactttttgc gattcgctta
     2941 attgagcttt attatttttt aggtcttcca gttttgtttt catcgcttca gaaatttctg
     3001 cttcgctgcc ttccaaattt cctgaaagaa gagatttccc ttctgcttcc acttttacaa
     3061 attcctggcg catccctaac gaaacagcaa tcatttcttc cgctattttg gaatcagctc
     3121 tggcaatttg atagttatga ttgatgattt tgaaattggt agttttgggg gttagtaaag
     3181 cggtggcaaa agattccaag gaaagcacag gcagcttatt ataatattcc ggattttcca
     3241 attgagcatc atcaagatat aattttatct ctacttcctt ttttttcttt ttggcatcca
     3301 gagctgtatg caaagaaagc attgaaagca ggatagcgag aattaataag attctggata
     3361 atctcatata attcttatgt tcaatttcca agctttacgc gaagctggtc aactaaatct
     3421 tgggaggcat tttttgcagc ttcaattaag gcattagttt tggctacaga aggattggag
     3481 ccaataccgc taaactgata aggtcctgca ctgcatactt ttactgcaaa ttttttgcgt
     3541 aggtctaaaa tatagccgtt tacttttaca taaaccttac tacctccaga aacaggatca
     3601 atttcttctc tgccaacatc taacattgct acagctagaa aatctaaacc tgcttcgcga
     3661 gctatatcag tagcttcttt ttgggtggaa gaggagattt catttaaggc agcaaaatcg
     3721 taggcaaatt tttcgggtga aatgcctact tcataagcgg gaacaacttc aaaattggct
     3781 ttattgaata tctcggttac ttttgtatct atttccccag ggttaaaagt gcggtaatag
     3841 attccctcac tggcatttac tacagattga gaataaacca tagaaccttc tcgggagata
     3901 cttttatcaa tattcacttc agcttggctg cttgtgctta ttttattagg gtcggataaa
     3961 ttgctatcgg tatctccgct ggtggcaatt tttcctgagg cagagacatc cgatttggta
     4021 gaaaggtctt ttttaatagt ctgtccttgg ctttctacag ctgcaacttc acgagctaca
     4081 tacacaaagc ttaaatatgt tggttcagcc atttccatta catctttcat tttttggtta
     4141 attagcaatt ccagctgaac atcgtcgatg gtaacttcca acaattgttc ccaaatgccg
     4201 tttgttttgt ttccttcgtt taagtaattt atttccagga tgtaattatc aatattcttt
     4261 tctatatccg gcatcagttc gttgacaagt tctcttttgc gtgtgtcttt ttcggaggcg
     4321 tattttttaa cagccagaat tttagcatta ttaacagcag ctttaatttc ttcaggtttg
     4381 ggcttgttct ttttggtttc tacggtggct ttaactttca ctttgacagc tgcttccaga
     4441 taagctaccg aaaagagcat tgtaacgcta agcaatataa tagttaatag tttgcgcatt
     4501 tttttaatat ccccttcatt aattttttat cttctttcag tgtggcgggt attttgtccg
     4561 acagcgttaa aagaagaatt tcgttgtatt tataaaactc attgccgatt atttgtccga
     4621 caacggtttg tttagcagca ccattttgaa tagtctgttt ccagtattct tgattaaact
     4681 cggcatcgta aatacgcatt tctaattcca caccataaag atcaactctt tctgcggcag
     4741 tttctttgta aagcttttta agaaagcgtt taacagtgat atctatatcg taactggcag
     4801 cagggatagt aaaattctga atgctggcat cagaaaagga aagagacatt ttgtatgata
     4861 aattactgcg agaataaggt aaaacggtta aattcatttt aaaagcaagt gcctctgtta
     4921 aatgctgagc tacaatagcg gcataagttt caatttctgg ttctgtttta tatgaaccaa
     4981 gaaacggcaa tgcttcttcc tggaaattca cattggcaac tttgatagtt agcgaaccag
     5041 aagaacggat atagatgttt ttttgattac tcatcagctt ctgtaaaatg taattggaat
     5101 agagtttttt tatagtttgg ataattgttt gctgatcggg ctctcctttg taaagatcaa
     5161 tataagtaac atttatggga taggactgga gaatagtaag atccgcaaaa tccattataa
     5221 aaacagtgca gcccaaattg taaattgcct tggtttcatt atcgtaatat tcaaaagcaa
     5281 cttcttcccg gtttaaagct attgccagag atagggttgc cttggtttga ttgtgaatat
     5341 ctaatgtaaa attacctagc tcggaatttg cagaaaaata gtcaaaaaaa agcttatcta
     5401 aataggattg agcggaggaa ctatcctgaa gcattttttg agtgtatgga tagtttttgc
     5461 ttatatcggc ataagcacca ctaaaacaaa agccagcata tttaacatct tgattggcaa
     5521 ataataaacc tgctgccaga gtgaatatga acgctatttt tacatttttt cgcataccta
     5581 tatttttaac catcccctat aagtacatct ttctttaaat tttcctatag tttattctgc
     5641 tatttctgtc aagaaaaaga tgtttagata acttcagcag atatttttta gccccagaaa
     5701 acaccgaata aggtaaagcc tataaattag attataactt taagctccca cactacaaac
     5761 taacaacttt tatagttacc gcaaatataa gtcctcaccg ccaaacacaa gatagagatg
     5821 ttcataagat atttgggcag tgataactct cttgttatag atattcttta tagataatga
     5881 tcttagcagc gtattgttta tgaagttcag ccaaatgcct aatcagcaag tattcttttt
     5941 caatttgttg gacgcgggca tacaaagaat ctttaatttc ttcgaagggg atataatctt
     6001 tttcgctatg acctgttttc aacaggatgt gatagccata tttagaaggg aaaggctggc
     6061 taatttcgcc aacttttaaa gcaaaggcaa cttcctcaat ttcgggaatc attattcctc
     6121 tggagaacca tcccaaatca ccacaggagg aattactaag acaatcggaa tattctttac
     6181 agtagtagcc aaagtcctcc gcgttatgta tttttttgcg gatctcttct gccagggctt
     6241 ttgcatcttt tttcgtgtta ctgatcagga tatgtgaaca gcgaacctgc tctgctttca
     6301 aaaaatactc cttttgttcg tcgtagaatt cttttacttt gtccttagta acaggagtat
     6361 catgcgagaa gaagctattt atatatttgt ttatcatcaa tcgttctttt aagaggtttt
     6421 ccatctcttt gggggtgagc tgatctaagg aagtgctcca caggtctaag ggattttctt
     6481 catctaagag ttcccaaatt gctttatcgt attcggcatc gctgattgtg attcctgatt
     6541 ctatagcttt ataaagcaag aggtatctat caattaaacg tttaagcgca ttttgcaggc
     6601 attctttccg ttctgtaata ctaactttgg cacattcgcg ttctaaatcc tgttcgctta
     6661 tctcaaattc aaatactttc gctactattg gcatttttct tttttagacc ttccttaatc
     6721 ttggtgacta tattgtatag tataatcgaa gaagagtcaa ttgtcaaatc gaaagtgtat
     6781 ttacgatacc aagttacctg tctttttgca tagttgcgtt gatgttgagc tgcaataatt
     6841 gcacaatcct ctatgcttgc ttcttctcta aaatagggca gaaactcttt atagccaagg
     6901 cattggagtc ctggtgagtg atctgtataa cccattgtaa agaggttttc aatttcttgc
     6961 agtaagccct cggcaagcat tttttccatt cgttcattaa ttcgtgtata tagttgggga
     7021 cggggcatat cgatcaaaat tcggaaggca ttatattttc cagtttcttt ttgcttgcgc
     7081 cagtgttcag aaaggggaat tcccgttcct gaaaaaactt ccaagcctcg caaaattctt
     7141 tgtgtgtcat tttcactgat cttagaggca aattctgggt ctatatttag cagctcggaa
     7201 tatagagagg taagtccctc ttccttaagg cgttgcttta aatcgtatct tatttttgtg
     7261 ggtaagggag gcagggtaaa aagcccattt aataatgcct ttatatataa acccgttcct
     7321 ccacagatta ttggtatttt gccttcagaa tgcagcgaag tgattatttt ttctgcgtcg
     7381 gctgcaaaag aaccggcatt ataggtttcg tcgggattga taatatcaag caggtgatgt
     7441 tttgctcgtt gttgttcagc tttagcaact tttgcagttc caatatccag atagcgatag
     7501 acctgtctgg aatcggcaga aatgatttct gtgcataatg attcagctaa tgcaatagct
     7561 aatgcagatt tcccagaagc ggttgcccct tcgatagtga taacaggaat catactatgc
     7621 gtttaaactt tttctcgaaa tcggagaccg gtatttttag aatcaagggt ctgccatggg
     7681 gacaaaaata gggaagctta caagcaaaga gatcattaat taaattcaac atttccttgc
     7741 gggtgagttt atgattggct tttatagcag ctttacaagc aattgatttt gccagacctt
     7801 ctttgaaatc ggtagcaatt tccatctcct gttccagttg tttcagaatc tcgataaata
     7861 tctggccact ggcaaaatcg gataactcag caggaatttc ttcaatcacg atagaatcac
     7921 cactgaattt ctttagggtg aaacctacct tttcaagcag ttccagattt tcttctacca
     7981 gctctttaat atcagatgca atatagggag gtatatcaat aaccagagga ataattaatt
     8041 tttgacgcat agcgggggct cctccggtac gctgtaaaag tttctcatag attattcttt
     8101 cgtgagcggc atgctgatca ataataacta agccatcttc tacttgaata aaaatatagg
     8161 tattatgaaa ctgccaggga ttgatatagt cctcttcatt tttcagcagg atatcgtat
//
LOCUS       DAORHB010000045         8201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_8291_length_8201_cov_4.400442, whole genome shotgun sequence.
ACCESSION   DAORHB010000045 DAORHB010000000
VERSION     DAORHB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8291_length_8201_cov_4.400442"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1016
                     /locus_tag="PKM80_02385"
     CDS             105..1016
                     /locus_tag="PKM80_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNV92784.1"
                     /translation="MNIFLGIDIGGSSFKYGLGNCEVGLQYYQILNLPEKNLNTFLSV
                     AAKMFNDVDNQFGLNKLKGIGIGIPGAIDKITGCAIGTNPNLPFWVNHSPLELIPDNC
                     KLPLAFDNDANLMALSEANSYCKQYAIGVTIGSGIGCGIISDGQILHGANGYAGELGH
                     ICVVDNGAKCNCGKNGCLEAYSSVEGLRNLLAIHNPLYSKLDLPTLVAKKETDPLVNE
                     YCKQGKQLLSIALAGLATCLDPEIIILGGGAMDFDLYYISEIEKDILELLPKAHRAKI
                     SITKAKNGNKAGVLGAIKLIEEKFYGN"
     gene            1064..1420
                     /locus_tag="PKM80_02390"
     CDS             1064..1420
                     /locus_tag="PKM80_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92785.1"
                     /translation="MRKIMLTMVLMAVLMMLATAGYALKSSPVECFDISPNPMYKDCE
                     IYVAVSAPLSINIQIQSLDGKVIRDIYSGLVGKDITLYWERTDNWGNYVPDGEYFVVL
                     GFSTRYTSLKKTLILK"
     gene            1451..2212
                     /locus_tag="PKM80_02395"
     CDS             1451..2212
                     /locus_tag="PKM80_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV92786.1"
                     /translation="MQYKRKKSLTDFFLFLEEATTELFHIRGNRHVSLMVVIRQILFT
                     GVEALPLIGFIALAIGCLTIMQGYAFLSNFGQGIWVHIILVRVIVGELSGLITALVVI
                     ARSGTAISTELGNMVVNREMQLLKSFDIAPMGYLVVSRILGVVISMLVLTLYFNIIAV
                     LGGWLFAQFFNHLSFSAFMNDFFSILTFPHIIMTVLKSIVFGFVIAITASYQGMSVSK
                     ASTEVPQRTIKAVVNSIVAVIIVDIIISWLFWIGV"
     gene            2212..2919
                     /locus_tag="PKM80_02400"
     CDS             2212..2919
                     /locus_tag="PKM80_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV92787.1"
                     /translation="MHIAFENIVIKGLVNNLNLEFDTKGCTVLYDPCEIVSSTILSAL
                     IGLYELDGGNIYLDGIPKQEYFLNHSLISTFSMIFNEGIMLANLTVKENLLLPYHRRF
                     QIVETSIFEKELKFFMQVFNQDTDLELRPAFLTSSQRKFFCFIRSLLLKPKILLIDDP
                     YYLFNRNDRKQIYDFLKRFSSSSGIDIDFATKHNNIQDSYNYEKNGIPEMLISTSDED
                     FTDNFATQVIDLSQFKS"
     gene            complement(2916..5351)
                     /locus_tag="PKM80_02405"
     CDS             complement(2916..5351)
                     /locus_tag="PKM80_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNV92788.1"
                     /translation="MRKTTFCLLLLMLSISLSFGQYIVNFEGADETKTAYASGDVTLS
                     GLSWNLTEVLIGNLATDWKNGTKSARLRGYGTSAMTMLENKTSGLGTLSFYYRRYATD
                     AQVDWKVEYSIDNGSSWMQIGSVFTAPASDTPSLFSEDVNVSGDVRIRIQRATETGTS
                     NNRLNIDDITLTDFTGIEVEPPTLQASNIIAYPANTEITLEWTPGNGARRLVKINTIN
                     SFTSPVDGSNLAANDVYSGIGEQVIFNGSTQIVEGLPLNGCNVSGLTPLTTYWFRIYE
                     YNGSGSFTRYNTSESTNNPISAITTNTPGSDYYTGITGYGITLKTNLHNLLRTTHTTR
                     YSYDALWIKLPYTDEDPTNTNNIIEIYTGWSVPKTYYGSGTSQWNREHTWSKSHGNFS
                     ETPPAGTDLHHLRPCDSTVNSAKSNKDFDYATTSYYDSSPYNGYPQDTGCKTSTYAWE
                     PRDADKGDVARMIMYMAVRYEGTDTSYDLEIVDYTNTSGPYYGKLSTLLEWHIIDPPD
                     SIEMERNNRIEELQGNRNPFIDEPLYAVQIWAPMPQNPTNITQTSFTANWSAPISATK
                     YYLQLATDASFNNIVPGYDNLDAGLILSKTISGLSNTNTYYYRLRSFFVSGYSMYSPY
                     MEVTLSASAQLNSGTALTEYNLNAAILQLNLVDTTWRDNILMLANFTINNAPDGLSLL
                     SVQYLSSNSALLVMHFNNADFDDNILDFCVTIDAAEINYPSSITTNSLAIIAYVESPL
                     TIQIQGQQLILNIQTVAEADAYIVFSSIEPYGIYSEISSSGNFDAQIPTRWTCILPTE
                     NIRFYKAAAVKYP"
     gene            5539..6099
                     /locus_tag="PKM80_02410"
     CDS             5539..6099
                     /locus_tag="PKM80_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92789.1"
                     /translation="MERIAFILLITLLLCSCDLFTVRDSEHPGAETPWIDFATECDSV
                     LLNLEYSYEYVQNAVHYSGIFTPDYVFHFAPQDISDLSIEQTWTRVQEQDMIQVLHNR
                     YKEMQVTLEPVETQDQIESTQAKIYRQYLITGIFRSEGKERLVLASGNLELHLKKEYG
                     YWYLNQWYDYRSSNSPTWGRLKYENH"
     gene            6086..6661
                     /locus_tag="PKM80_02415"
     CDS             6086..6661
                     /locus_tag="PKM80_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92790.1"
                     /translation="MRTISIILIFLTFLIGSCSNPFCPELNDVSSAEIQNRTPEELLQ
                     NLERAYTEKNINLYKSLLHPDFRFELLASEVSLIGIDMNGDGMRDSWWGYDQEIEYTD
                     RMFNRGSSDGTYYPPDEIKLRLQIPPSDRWQTDPEVGHEDWIIIPCNFDLILSYKESN
                     SLFNANGIARFYLKPVKNGWQIAIWRDESNI"
     gene            6658..7074
                     /gene="ruvX"
                     /locus_tag="PKM80_02420"
     CDS             6658..7074
                     /gene="ruvX"
                     /locus_tag="PKM80_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNV92791.1"
                     /translation="MNPEGRILALDYGEKRIGLALSDPNRIFAKPLCILSNKGFEQLI
                     CELQEIIVNNQVTMLIVGIPYAIDGSFTAKTEECQSVLDRLQEVLTIPVEGFDERYST
                     WEANEELKKMGYNWQQARKIKDAMAAAIFLREYLSK"
     gene            7071..8054
                     /gene="mltG"
                     /locus_tag="PKM80_02425"
     CDS             7071..8054
                     /gene="mltG"
                     /locus_tag="PKM80_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNV92792.1"
                     /translation="MNKKKVVIISLAIVVFLVMLYIAYLIFVPKHSEELIVRIKPGDN
                     ARVIANKLSESGIIRSKTMFIWLTKLRNADRNLKPGSYIFGGNTYLWQTVTRLYKGQS
                     ENITITFPEGLSLYKTLKRIDASGLATFEELSAKAKDPTVVNKLTGFDVPSLEGFLYP
                     ETYRFPIDISADSILAIQTAEFFKKLNQEGIDPYSTPNFYNKLILASIVEKEAGTESE
                     KEIIAGLFMNRMAIGMALQSCSTVDYLLEPKGIKRDVLTYTDTQINSPYNTYIYQGLP
                     PTPICNPSVNTIKAVLNAKPNSYLYFFSDRKGKNVFSRTYEEHLAKQRQML"
BASE COUNT         2545 a         1379 c         1564 g         2713 t
ORIGIN      
        1 accggattgt ggttccgggc gttgtgggtt caaatcccat cacccacccc attttttttc
       61 ttagcgatct tactgtaaac aaaatgaata gatgaagcaa aattatgaat atattcttag
      121 gcatagatat tggcggttcc agttttaaat atggtttggg caactgtgaa gtaggtttac
      181 aatattatca gatcctcaat ttaccagaga agaatttgaa cacattttta tcagttgctg
      241 ctaaaatgtt caatgatgta gacaaccaat ttggtcttaa taaattaaaa ggtattggaa
      301 ttggcattcc tggcgctatt gataaaatta caggttgcgc tattggtaca aatcctaatt
      361 tacctttttg ggttaatcac agccctcttg aactgattcc cgataactgt aaacttccct
      421 tagcatttga taacgatgcc aatttgatgg ctttgtccga agccaatagt tactgtaaac
      481 aatatgctat tggagttacc attggtagtg gtattggttg tggaataatt tctgatggcc
      541 aaattcttca cggtgcaaat ggctatgctg gagaacttgg ccatatttgc gttgtagata
      601 atggagcaaa atgtaattgt ggcaagaatg gctgtttgga agcatattca tcagttgagg
      661 gactacgcaa tctattagct atccacaacc ccctgtactc taaattagat ttaccaactc
      721 tagtggctaa aaaagaaact gatcctttag taaacgaata ttgtaaacaa ggtaaacagt
      781 tattatctat tgccttagca gggcttgcca cttgtcttga tcccgaaata ataattttgg
      841 gtggcggagc aatggatttt gatttatatt atattagtga gatagaaaag gatattttgg
      901 agcttttgcc taaagcacat agagctaaga tatcgattac caaagcaaaa aacggtaata
      961 aagcaggagt tttaggtgct ataaaactta tcgaagagaa gttttatggt aattaggaat
     1021 gaatgttgca taatatattt acatcaataa ttaggagtaa aaaatgagga agattatgct
     1081 tacgatggtg ctgatggcag tgttaatgat gttagccaca gctgggtatg ctttaaagag
     1141 ttctcctgtt gagtgttttg atatttctcc caatcctatg tataaggatt gtgagatata
     1201 tgttgctgtg tctgctcctc tgagtattaa tatccaaatt cagagtttag atggtaaagt
     1261 tattcgtgat atttacagtg gtttggtagg caaagatata actttgtatt gggagcgaac
     1321 tgataattgg ggcaattatg taccggatgg tgagtatttt gtagttttgg gatttagcac
     1381 ccgttatacc tctttaaaga agacacttat tctcaaataa caacgctttt tttaagcgat
     1441 tatgtaactt atgcaataca aaagaaaaaa gtccttaacg gacttttttt tgtttttaga
     1501 agaggcgaca accgaattat ttcatattcg aggcaatcgc catgtaagtt taatggttgt
     1561 catcaggcag attctgttta caggtgttga ggcattaccc ttgataggtt ttatcgcttt
     1621 agctattggg tgtttaacga ttatgcaggg ttatgcattt ttaagcaatt tcggacaggg
     1681 aatttgggtt catataattt tggtaagggt tatagtgggg gaattaagtg gtcttattac
     1741 agcgttagta gttattgcca gaagtggaac tgcgatttct acagaattgg gaaatatggt
     1801 agttaaccgg gaaatgcaat tattgaaatc ttttgatata gcaccaatgg gatatttggt
     1861 tgtttctcgt attttgggag ttgtaatttc tatgctggtg ttaacattat atttcaatat
     1921 cattgcagtt ttgggtggtt ggctttttgc gcagttcttt aatcatttat cctttagtgc
     1981 gtttatgaat gatttctttt ctattcttac ttttcctcat attataatga ccgttttaaa
     2041 atctattgtt tttggttttg taatagcaat tactgcttcc tatcagggaa tgagtgtttc
     2101 caaagcatca actgaagttc ctcaacgcac gataaaagct gttgttaatt ccattgtagc
     2161 tgtaattatt gtagatatca taatctcttg gctgttttgg attggtgtgt aatgcatatc
     2221 gcttttgaga atatagtaat caaaggttta gtgaacaacc tgaatttgga gtttgacact
     2281 aagggatgca cagtattata tgatccttgt gaaattgtat cttccactat cttatctgct
     2341 cttattggac tttatgaatt agatggcggt aatatatatc tggatgggat acctaagcaa
     2401 gagtattttt taaaccactc tctcatttct actttttcta tgatttttaa cgaaggaata
     2461 atgctggcca atctaactgt gaaagaaaat ttgcttcttc catatcatag gcggtttcaa
     2521 atagtggaaa caagcatctt tgaaaaagag ttgaagttct ttatgcaggt ctttaatcag
     2581 gatactgatc tggaattgcg tcctgctttt ttaacatctt cccagcggaa atttttctgc
     2641 ttcattcgtt cgttgctctt aaaacctaag attttattaa ttgacgatcc ctattatctt
     2701 ttcaatagaa acgataggaa acagatatat gattttttaa agcggtttag ttcttcctct
     2761 gggattgata tcgatttcgc tactaagcat aataacatac aagatagcta caattatgaa
     2821 aaaaatggaa ttccagaaat gctgattagt accagtgatg aagattttac cgataacttt
     2881 gccactcagg ttatagacct ttctcaattt aaatcttaag gatatttaac cgcagctgcc
     2941 ttataaaatc ttatattctc agttggtaaa atacaagtcc aacgcgttgg aatttgagca
     3001 tcaaaattgc cgcttgagct tatctcagaa taaataccgt atggttctat gctggaaaaa
     3061 acaatatagg catcggcttc tgcaaccgtt tgaatattta aaattagctg ctggccttgt
     3121 atttggatag ttagcggact ttctacatag gcaatgatcg ctaacgaatt agtcgttata
     3181 ctagatggat aatttatttc ggcagcatct atagtaacac aaaaatccag aatattatcg
     3241 tcaaaatcag cattattaaa atgcataacc agcaaagcac tattagaact taaatattga
     3301 acacttagca aagaaaggcc atcaggagca ttatttatag taaagttggc tagcataagg
     3361 atgttatctc tccaggtagt atctaccaaa ttcaattgta agatagcagc attcaaatta
     3421 tattccgtta aagctgttcc agaatttagt tgtgcagaag cagaaagagt tacttccata
     3481 tagggagaat acatactgta gccactaaca aaaaatgaac gcaagcgata ataataagta
     3541 ttggtattgg ataagccact aatagttttg gaaagaatta aaccagcatc cagattatca
     3601 tatccgggaa caatgttatt gaaagaagca tccgtagcca attgcaaata atattttgtt
     3661 gctgatatgg gagcagacca atttgccgta aaactggttt gagtgatatt agtaggattt
     3721 tgtggcattg gtgcccagat ttgaactgca tagagtggtt catcaataaa aggattacgg
     3781 tttccttgca attcttcaat ccggttattt ctttccattt caatggaatc gggaggatca
     3841 attatatgcc attctaataa tgttgagagc tttccataat atggtccact ggtattggta
     3901 taatccacaa tttccaaatc gtaggaagta tccgttcctt cataacgaac tgccatatac
     3961 ataatcattc tggctacatc acctttatcc gcatctcggg gctcccaagc ataggtagat
     4021 gtcttacatc ctgtatcttg tggataaccg ttatagggag aagaatcata ataagaagta
     4081 gtagcataat caaaatcttt attacttttg gcagaattta ccgtagaatc gcaagggcgc
     4141 aaatgatgca aatcagttcc agcagggggt gtttcactaa aattaccatg acttttactc
     4201 caagtatgtt cccgattcca ttgtgaagtt ccgctgccat aatatgtttt gggaacactc
     4261 caacctgtat atatttcgat aatgttatta gtattagtcg gatcttcatc tgtgtagggt
     4321 aattttatcc acaaagcatc atacgaatag cgagttgtat gagtagtccg aaggagatta
     4381 tgcagattcg ttttcagtgt aattccataa cctgtgattc cagtgtaata atcactaccg
     4441 ggagtatttg tagtgatagc agaaatagga ttatttgtgg attctgatgt attatagcga
     4501 gtaaaactac cgctgccgtt atattcataa attctaaacc aataagttgt tagaggtgtt
     4561 aagccagata cattacaacc atttaaagga agtccttcta ctatttgtgt tgaaccatta
     4621 aagataactt gttctccaat accactgtaa acatcgttgg cagcaagatt gctgccatca
     4681 acgggagaag taaatgaatt aatggtattg attttaacta agcgtcgtgc cccattacca
     4741 ggagtccatt ccaaagttat ttcagtattg gcaggataag caattatatt tgaagcttgc
     4801 aaagtggggg gttcaacttc tatgcctgta aaatccgtta aagttatgtc atctatattt
     4861 aagcgattat tagaagttcc tgtttcggta gcgcgttgaa ttcttattct cacatcccca
     4921 ctaacattta catcttcaga aaataaactg ggtgtatcac ttgcaggagc tgtaaaaaca
     4981 gaaccaattt gcatccaact actgccattg tcaatactgt attctacttt ccagtccact
     5041 tgcgcatcag tagcatatct tctataataa aaagaaagag tgcccaaacc actagttttg
     5101 ttttctaaca ttgtcattgc agaagtccca taacctcgca acctagctga tttcgtacca
     5161 tttttccaat cagtagctaa attaccaatt aacacttctg tcagattcca agaaagaccg
     5221 cttaaagtta catcacctga agcataagca gttttggttt cgtccgctcc ttcaaaatta
     5281 actatgtatt gaccaaagct tagtgaaata cttagcatta aaagcagcaa gcagaatgtg
     5341 gttttccgca ttgagtaact ccctttgttt attatatatt atccttttcc cttgcttcct
     5401 caaattgtca agccaaatcc taaatttgtc tatggggtgc tctgtttttt tagcggtaca
     5461 caggactctt aattatttta gcatccaaaa tccacagatt ttgattgaca taatatttaa
     5521 aatccttttt atgaacttat ggaaagaatc gcttttattt tgctgataac tcttttgctc
     5581 tgtagctgtg atttatttac agtacgagat tcagagcatc caggagcaga aacaccgtgg
     5641 atagattttg ccacggaatg tgattctgtg ttattaaatc tggaatatag ctatgagtat
     5701 gtgcaaaatg cagttcatta ttcaggtatt tttacacctg attatgtttt tcattttgca
     5761 ccgcaagata taagtgattt gtctatagag caaacttgga caagggttca ggagcaggat
     5821 atgattcaag ttttgcataa tcgctataaa gagatgcagg ttactttaga gcctgtggaa
     5881 actcaggacc aaatagaatc cactcaagcc aaaatatatc gtcaatacct tataaccggg
     5941 atttttcgca gtgaaggaaa agagcgttta gttttggcta gcggcaatct tgaattacat
     6001 ttaaagaagg aatatggcta ctggtattta aatcagtggt atgattatcg cagtagcaat
     6061 tctccaacct ggggaaggtt aaaatatgag aaccattagc ataattctaa tattcttaac
     6121 ttttctgatt gggagttgta gcaatccctt ctgtcctgag cttaatgatg tttccagtgc
     6181 tgaaattcaa aataggacac cggaagaact tttgcaaaat ctggagcgcg catacacaga
     6241 aaaaaatatc aacctttata aatcgctctt gcatcctgat ttccgttttg aacttttggc
     6301 aagcgaggtt agtttaattg gtattgatat gaatggagac ggaatgcgag attcttggtg
     6361 gggttatgac caggaaattg aatatacgga tagaatgttc aatcgcggtt ccagtgatgg
     6421 aacttattat cctcccgatg aaataaaact gcgtttacag attcctcctt ctgatcgatg
     6481 gcaaaccgat cctgaggttg gtcatgagga ttggataata attccctgca attttgattt
     6541 aatcctatcc tataaagaaa gtaattcctt gtttaacgcc aatggcatag ccagatttta
     6601 tttaaaaccg gtaaaaaatg gctggcaaat tgccatttgg agggatgaat ccaatatatg
     6661 aatcccgaag ggcgaattct tgccttggat tatggtgaaa aaagaattgg gcttgcttta
     6721 agtgacccta atcgcatatt tgccaaaccc ttgtgcattc ttagtaataa aggttttgaa
     6781 cagcttattt gcgaattaca agaaattata gttaataatc aagtaacaat gctcattgtt
     6841 ggcattccat atgccattga tggttccttt actgctaaaa ctgaagaatg ccaatctgtt
     6901 ttagatcgtt tacaagaagt tcttactatt ccagttgaag gatttgatga acgctattct
     6961 acttgggaag caaatgaaga attaaaaaaa atgggatata attggcagca agcaagaaaa
     7021 attaaagatg ctatggcagc tgccatcttt ttaagagagt atttatctaa atgaataaga
     7081 agaaagttgt tatcatcagt ttggcaattg ttgttttttt ggttatgtta tatattgcct
     7141 atctgatttt tgtacccaag catagcgaag aattaattgt gcgtataaag cctggagata
     7201 atgccagagt aattgccaat aaactttccg aatcaggaat tattcgcagt aaaacaatgt
     7261 ttatttggct taccaaacta agaaatgcag atagaaacct aaaaccggga agttacattt
     7321 ttggaggtaa tacctatctt tggcaaaccg ttacccgctt atataaaggt caaagcgaga
     7381 acattacaat cacctttcca gaagggcttt ctctatataa aaccctgaaa agaattgatg
     7441 ccagcggctt agctactttt gaagaattat cagcaaaagc aaaagatcct acagtagtta
     7501 ataaacttac cggatttgat gtcccatctc tggaaggatt tttatatccg gaaacatatc
     7561 gttttccaat tgacatttca gcagattcca ttttggcaat tcagactgca gaatttttca
     7621 aaaagctaaa ccaagaagga atcgatcctt attctacgcc caatttctat aataaattaa
     7681 tccttgcttc cattgtggaa aaagaagcag gaacagaaag tgaaaaagaa attattgctg
     7741 ggctttttat gaatcgaatg gcaattggta tggctcttca atcttgttcc acagtagatt
     7801 atcttcttga gccaaaaggc attaaacgcg atgttttaac ctatactgat acccagataa
     7861 attctcctta taacacctat atttatcaag gtttacctcc tacgccgatc tgtaatcctt
     7921 ctgttaatac aataaaagcg gttttgaatg ccaaacctaa ttcgtacctc tattttttct
     7981 ctgatcgcaa agggaaaaat gtgttttccc gaacctacga agaacattta gccaaacaaa
     8041 ggcaaatgct ataaaatctg tgttatttgt gagaagtact tatatctctt tttcggtgat
     8101 ctcggtgccc tcggtggcta aaaaaatcct ctaaccctta aatccttaaa tccttatttc
     8161 taaaactaaa tgggtgtaat ttatgtagtc tgcgtgaatt a
//
LOCUS       DAORHB010000046         8175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_8326_length_8175_cov_4.302463, whole genome shotgun sequence.
ACCESSION   DAORHB010000046 DAORHB010000000
VERSION     DAORHB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8326_length_8175_cov_4.302463"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..938
                     /locus_tag="PKM80_02430"
     CDS             141..938
                     /locus_tag="PKM80_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92793.1"
                     /translation="MRCKYCNARLAIHDIWCMQCGRHTSALKNELFSIKSLSETYRAY
                     KVNMSSSLPGAAVAVILGLIPVAVLIWMFSSIIVLESNTTLQLLLNLLIKSIVFSIFI
                     PFMLIAFNPICKHNDYHLNLSEMFTALRCYPRYFLWTLINAVFFCIVYIICFGLPNFS
                     SAPILHLVWLILVNYWIAIVLPSLVLMEELSINPWKAIIKSYQHFQDVRWNIYLLALV
                     LAVLNALGCLFFWLLLIPEALILPLSFFAVRDYTRRLIEFELLTYRL"
     gene            949..1953
                     /locus_tag="PKM80_02435"
     CDS             949..1953
                     /locus_tag="PKM80_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNV92794.1"
                     /translation="MNRREIISLIILIVVIGFGAYKYLTRSFTELKSQYLMDTIVEIS
                     ATSQSKNVGRQIDSVFNFIRSLEDKLNEYDPNSYLGKINADSLNTVFDMDPDMYNLLT
                     IADSLFKMTNGAFDPTIKPVWDLWNFNAENPVPPDSLVLKEMLSKVDFTKIRYDEKRL
                     IKPLGMQLTFGAIAKGYILDKARDHMQTLNLDNGYINCRSSMTFFGYKKPQIVYIQHP
                     RKLDDYIASFKVLNQSISTSGDYQQFYEYNGVRYHHIISPFTGYPVPDVHSVTVLCSS
                     AAWSDGISTALFLLPPETVLDSIKQIADCEAIIYYSKADSLASIKTAGMKDKDLKEKL
                     "
     gene            1950..2720
                     /gene="folE2"
                     /locus_tag="PKM80_02440"
     CDS             1950..2720
                     /gene="folE2"
                     /locus_tag="PKM80_02440"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNV92795.1"
                     /translation="MSIPDIQSQPDTRKITIDKVGVKGIRYPIVVQDRENGIQHSVAD
                     LDIYVELPHQRRGTHMSRFLEVLNRYHTEVFIDKLDSFLNELKSTLKADTAYIDISFP
                     FFIKKQAPVSKVVSLLGYDCFFNASYNNNFELWIGVKVPVTTLCPCSKAISVSGAHNQ
                     RSEIFIKVRYREFVWLEELIQLAEEHSSCEIFPLLKRSDEKFVTDKAYSNPKFVEDIV
                     REITMELNKDKRITGFYVEAENFESIHAHNAYALISRI"
     gene            2728..5391
                     /locus_tag="PKM80_02445"
     CDS             2728..5391
                     /locus_tag="PKM80_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNV92796.1"
                     /translation="MNDKLITGNISFPKPICRNLSNGLEVISVKDNSHPVLCLQLYIR
                     TGSTLENDQQRGYSHFIEHLSFKSTSDFPNNKISLYASGLGGMLNAFTDYDCTCYYLN
                     LPSEKLKEGFHILSQLAFHSTFSKEDVSKEKEIILEEIKQYQNDPESDFLEYIQTNCY
                     QKSPLRYPVLGNPKSINSARYKELREFYKNRYTPVNSFLVICGNYQENELDAYLDTYF
                     SAWENISKPLPYLLDIEPEMNGFRYIFRKKAIGDDTIAFAFPELSEKHPLANALLIAM
                     RYFAIGRSSRLYNRLVEKDKLCSSVKVNSLCGVLSGVSVIYITPISATFIPDIIRIVY
                     EEYSALLQFGIPLAELELIKKDIINSWLFSFEGMENLANLIAAEKFTGDLCRLQKYGD
                     EINSISIEAVMAAIHKYWLPEGITVYHEGSCEISGTKKQAKQIKESLAPKKTILCAEE
                     IHNISIQNLNPPPLYNAENTRRGYITQIDDNYYQIVLSNNVKVIFKQQKNNNISGFSL
                     STPISQISETPSEIGHNFFCTSLQLYKTQKHSHQELLQYSRNNGFNIRLIHHLDTTTF
                     RGKCLGSNLDKALAMLAESIFLPSFDHNYFSVLTSTALDGIRRDNDYPVSYAYMNWYK
                     MLVGNKSNLFRPTGSATQIRSIRIKHLQQWYEDWNIGKDFCLAIVGNHNPQEILELCE
                     KNFGLVHNVSHSPKFKPFYSPSSVHFKKTYRNTDQAIIYIGGFASPAIDRDENTAFYI
                     LSQILGGDISSRFFDILREKYAYAYQTGFDFHSLNELGFWNAYAFCDKDDYNNCLALM
                     KEIIYSIADKGVKENELETAKQYLIGMNRFEDESVSYTASTISNLVALGYEPEYYLLR
                     EERIKKVNSELIWQIAKKWLLPDNQYIYIML"
     gene            5394..6158
                     /locus_tag="PKM80_02450"
     CDS             5394..6158
                     /locus_tag="PKM80_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV92797.1"
                     /translation="MFKLGIDIGSRNTKVVIFNEHTKSIEFSDCKSTEIFILSGVNKL
                     LHNAFLASGINKNELTAIGVTGYGRKLFKDATLTISEISCHTAGCLYYFPDAKTIIDM
                     GGQDSKIITLTDSGKVKDFVMNDKCAAGTGRFLEMTAMRLDCDVSDLSAIASKSTKEL
                     KLNSTCVVFAESEIIGMLSSSTAPENIVRSVHRSIAKRILAQMASLVWEPPIVFTGGV
                     ALNQDIVKCFSESLSYQLITPPDPEITAALGAAILA"
     gene            6158..6901
                     /locus_tag="PKM80_02455"
     CDS             6158..6901
                     /locus_tag="PKM80_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNV92798.1"
                     /translation="MKFDYHIHTEDSYDSSIKTIDLIDRAIQLKYDEIAITEHLDLLP
                     IEMKSYGAPSLKHYFKRINDLQNTISELTLLSGVEVGDYHIVKDYAVSLLEGINFDLI
                     LGSVHFLSDQTNVAIPLKKQLSEEQIRDYYLQNLALVSNCDIDVLAHLGVYKRCYNSI
                     PDERIHYPIIQDIFRVIIDRKIALEINYSSLRRGYPSFIPEQHILELYLALGGQLFSL
                     GSDAHFIEHFDLFRDSIPQEFSNSFRYHL"
     gene            7162..7434
                     /locus_tag="PKM80_02460"
     CDS             7162..7434
                     /locus_tag="PKM80_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HNV92799.1"
                     /translation="MNERCHILTIIMEDRESAFHPVNELLHSYAPHILLRVGYPMRDL
                     GVAVIFLIVELPIAEMGSFSGKLGQIKSVKVQVTTLKLEKGEKNEH"
     gene            7424..>8175
                     /locus_tag="PKM80_02465"
     CDS             7424..>8175
                     /locus_tag="PKM80_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV92800.1"
                     /translation="MNISTEGVKSFIPTTEIEEHLARNRFADTIRIRDIIDKSLDKQR
                     LNPDETAALINVTDPELKQQILEGAHTLKERIYGNRIVLFAPLYVGNDCINDCVYCGF
                     RSSNKECHRATLSKEELISETEALEDAGHKRLIMVYGEHPKYSPEFIADTVQTVYNVK
                     HNKGEIRRVNINAAPLDIEGFRIVKSVGIGTYQIFQETYHEETYKTLHPRGPKSNFLW
                     RLDSLDRAMQAGIDDLGIGALMGLYDWHFEVMG"
BASE COUNT         2592 a         1386 c         1459 g         2738 t
ORIGIN      
        1 cttgtggttg gagtaaatgt tagccagtgc ttgacaaaag cagcccttct gattttttgc
       61 ctcttacaga aatagcagat gatacattta tctgctattt cccttaaaag tgattcagaa
      121 attaaataaa aggagtatga atgcgttgta aatattgtaa tgctcgcctt gccattcatg
      181 atatatggtg tatgcaatgc ggaagacata catctgcttt aaaaaacgag ctcttctcaa
      241 taaaatcctt atctgagaca tatagagcgt ataaagttaa tatgtcctct tccctaccgg
      301 gagctgctgt tgctgttatt ttaggtttga taccggtagc agttttgata tggatgttca
      361 gcagtattat cgtattggaa agcaacacta ctttgcaact tctattaaac ctacttatta
      421 aatcaatagt gttttccatc tttatacctt ttatgttaat tgcttttaat cctatttgta
      481 aacacaacga ttatcatctc aatctttctg aaatgtttac tgctttacgt tgttatcctc
      541 gttatttttt atggactttg atcaatgcag tatttttctg catagtatat ataatatgtt
      601 ttggcttacc taatttcagc tcagctccca ttttacattt ggtctggctt attttggtta
      661 actactggat tgccatcgtg ttaccctctt tggtgttgat ggaggaatta tctatcaacc
      721 cctggaaggc aataattaaa tcctaccagc attttcagga tgtccgttgg aatatctatt
      781 tacttgcttt agtgttagct gtattgaatg cccttggttg tctttttttc tggttactgt
      841 taattcctga ggccttgatc ctgcctctat cattttttgc agtgagggat tatacacgtc
      901 ggctaataga atttgaattg ttaacctaca gattgtgagg tgttattaat gaatcgtaga
      961 gaaatcattt ccctaataat cttgattgtc gtaattggtt ttggagcata taaatatctt
     1021 acgcgctctt ttactgaact taaaagtcaa tacctgatgg atacgatagt agaaatatct
     1081 gccacttcgc agagtaagaa tgttggccgt cagattgatt cagtattcaa ttttattcgt
     1141 tctctggaag ataaactcaa tgaatatgat ccaaacagct atttgggaaa aataaatgct
     1201 gattctctta atactgtttt tgatatggat cctgatatgt ataatttact tactatagct
     1261 gatagcttat tcaaaatgac gaacggcgct tttgatccaa ctataaaacc agtttgggat
     1321 ttatggaatt ttaatgcaga aaatcctgtc cctcccgatt ctctagtact gaaagaaatg
     1381 cttagcaaag ttgattttac taagatacga tatgatgaaa aaaggctgat aaagccactg
     1441 ggaatgcagt taacttttgg agctattgca aaaggttaca ttctagataa ggcacgcgat
     1501 catatgcaga cgctaaattt agataacgga tatattaatt gtcgcagttc tatgaccttt
     1561 tttggttata agaaacctca aattgtttat attcagcacc ctcgtaaact ggatgattat
     1621 attgccagtt tcaaagtgct aaatcaaagc atatctactt ctggtgatta tcagcaattt
     1681 tacgaatata atggtgttcg ctatcaccat attataagtc ctttcaccgg ttatcctgtt
     1741 cccgatgtgc attctgtaac tgttctttgt tcttctgcag cttggtctga tggaatatca
     1801 acagctcttt ttttgttgcc tccggaaacg gttcttgatt caattaagca aatagcagat
     1861 tgtgaagcaa ttatttatta cagcaaagcc gattctctgg catcgatcaa aactgcaggg
     1921 atgaaggaca aagatctaaa agagaaatta tgagcattcc cgatattcaa tcccaacctg
     1981 acacccgtaa aataactata gataaagttg gtgttaaagg tatccgctat ccgattgttg
     2041 ttcaggatag agaaaatggc attcagcata gtgttgccga tttagatatc tatgtagagc
     2101 tacctcatca gaggcgtgga acacatatga gccgttttct ggaagttcta aatcgctatc
     2161 atactgaagt ctttatagat aaacttgatt catttttaaa cgaattaaaa agcactttaa
     2221 aagctgatac tgcctacata gatatcagct tcccgttttt tataaaaaaa caggcaccgg
     2281 tttccaaggt agtttctctt ttgggttatg attgcttttt caatgcatca tataacaata
     2341 attttgaact gtggataggt gtaaaagttc cagtaactac tctttgccct tgttccaaag
     2401 caattagtgt ttcaggagct cataaccaaa ggtctgaaat atttatcaaa gttcgctata
     2461 gggaatttgt ctggctggaa gaattgatcc aacttgccga agagcattcc agctgcgaaa
     2521 tttttcccct gctaaaacgt tctgatgaga agtttgttac tgataaagca tatagtaacc
     2581 ctaaatttgt ggaagatatt gtgcgcgaaa ttactatgga attgaataaa gataaacgca
     2641 tcacaggatt ctatgtggaa gctgaaaact ttgaatctat tcatgctcac aatgcttatg
     2701 ctttgataag tagaatataa catcgttatg aacgataaat taattactgg aaatatttct
     2761 ttccccaagc caatatgcag gaatttatct aatggcttgg aagttatctc cgtaaaagat
     2821 aattcccatc cagttctttg cctccagctt tatataagaa ctggttccac cttagaaaat
     2881 gatcaacaaa gaggatattc tcattttata gaacacttaa gctttaaatc taccagtgat
     2941 tttccaaata acaaaatatc gctttatgct tcgggtttgg gtgggatgct caatgctttc
     3001 acagattacg attgtacttg ttattattta aacttacctt cagaaaagct aaaagaaggt
     3061 ttccatattc tttctcaact tgcttttcac agcactttca gtaaagaaga tgtaagcaaa
     3121 gaaaaagaaa tcattctgga agaaatcaag cagtatcaaa acgatcctga atcggacttt
     3181 cttgagtata ttcaaacaaa ctgctatcaa aaaagccctc tcagatatcc tgttttaggt
     3241 aatcctaaaa gcattaacag cgcaaggtat aaagaacttc gcgaatttta taaaaatcga
     3301 tatactccag ttaattcctt tctagtaatc tgtggtaact atcaagaaaa tgaattggat
     3361 gcttatcttg atacttattt ttctgcttgg gaaaacataa gtaagcctct tccctatctg
     3421 ttagatattg aaccggaaat gaatggcttt cgctacatct ttaggaaaaa ggcaattggt
     3481 gatgatacta tcgcctttgc ttttccagaa cttagtgaaa aacatccttt ggctaatgca
     3541 cttttgatag ctatgcgtta ttttgccata ggaagatctt cgcggctata taatcgctta
     3601 gtagaaaaag ataaactatg ttccagtgtg aaagtaaatt ctctttgtgg agttctttcc
     3661 ggagtttcag ttatttatat cactcccata tcagcaactt ttattcctga tattattagg
     3721 atagtttatg aagaatattc cgctctttta cagtttggaa tcccattagc agaacttgaa
     3781 ttaataaaaa aagatataat taatagctgg ttatttagct ttgaagggat ggaaaatctt
     3841 gccaacctga tagctgcaga gaaatttaca ggagatttat gccgccttca gaaatatgga
     3901 gatgagatta attctatttc tatcgaagcc gtaatggctg ctattcataa atattggcta
     3961 cccgaaggta ttactgttta ccatgaaggt tcttgtgaaa tttctggaac aaaaaaacaa
     4021 gctaagcaaa taaaagaaag tttagccccc aaaaagacaa tactatgtgc agaggaaatt
     4081 cataatattt ctattcaaaa tctgaatccc ccaccattat acaatgcaga aaatacgaga
     4141 agaggttaca taacccaaat agatgataat tattaccaga ttgtcctctc taacaatgta
     4201 aaggttattt tcaaacagca aaagaataat aacattagcg gcttttcctt atcaactcct
     4261 attagtcaga ttagcgagac tccttcagaa attggtcata atttcttttg tacttcttta
     4321 cagctctata aaacccaaaa acatagccat caagaattgc tgcaatattc tcggaataat
     4381 ggttttaata taaggttaat tcatcattta gatacaacca ctttcagggg aaaatgttta
     4441 ggtagcaatc ttgataaagc actagctatg cttgcagaat cgattttttt accaagtttt
     4501 gatcataact atttctctgt attaacatct actgctttag atggaatccg tcgtgataac
     4561 gattatcctg ttagctatgc ttatatgaac tggtataaaa tgctggtagg caataaaagt
     4621 aatctatttc gacctacagg gtctgcaacc caaattcgtt caatccgtat aaaacatctt
     4681 cagcaatggt atgaagattg gaatatagga aaagactttt gcttagctat cgtaggaaat
     4741 cataatcccc aagaaatttt agaactttgt gaaaagaact ttggcttggt tcataatgtt
     4801 tctcattcgc ccaagttcaa accattctat tctccttctt cagtgcattt taaaaagacc
     4861 tatcgaaata ctgatcaagc cattatatat attggaggat ttgcttctcc tgctattgat
     4921 cgagatgaaa ataccgcttt ctatatttta tcccaaattt taggtggtga tatctcttct
     4981 cgcttttttg acattctaag ggaaaaatac gcttatgctt atcaaactgg tttcgatttc
     5041 cattctttga acgaattagg tttctggaat gcatatgcct tttgtgataa agacgactat
     5101 aataattgcc ttgctttgat gaaggaaatt atctattcta ttgctgataa aggagtaaaa
     5161 gaaaatgaac tggaaactgc aaaacaatac ttaattggga tgaatcgctt tgaagatgag
     5221 agtgtgtctt acacagcatc aactatttcc aatcttgttg ccttgggcta tgaaccagaa
     5281 tattatctct tacgcgaaga acgtattaaa aaggttaata gcgaattaat ttggcaaata
     5341 gccaaaaagt ggctattgcc tgataaccaa tacatctata ttatgttgta actatgttca
     5401 aacttggtat cgacatcggc tctcgtaata caaaagttgt gatctttaat gaacacacaa
     5461 agagtattga gttcagcgat tgcaaatcaa ctgaaatatt tatccttagc ggagttaata
     5521 aacttttaca caatgcattc cttgcttcgg gaattaataa aaatgaacta actgctatcg
     5581 gagttacagg ttacggtcgg aaacttttta aagatgcaac cttaactata tccgaaataa
     5641 gttgccatac cgccggttgt ttatattatt ttcctgatgc aaaaacgata attgatatgg
     5701 gtggacagga ctccaaaatc atcaccctta cagacagcgg aaaagtaaaa gattttgtta
     5761 tgaacgataa atgtgcagct ggaacaggac gctttttgga aatgactgca atgcgtttgg
     5821 attgtgatgt ttctgatctt tctgctatag cttccaaatc aactaaagaa ctaaaactaa
     5881 attccacttg tgttgtgttt gccgaatcgg aaattatcgg aatgttatca tcttcaactg
     5941 ctccagaaaa tatagtccgt tctgttcatc gaagtattgc aaaacgtatt ttagctcaaa
     6001 tggcatctct ggtttgggaa ccacctattg tatttacagg aggtgtagct ttaaaccaag
     6061 atatagtaaa atgtttcagt gaatcacttt cctatcaatt aattacacct cccgatccgg
     6121 aaattacagc tgccttaggt gctgctatct tagcataatg aaattcgatt atcatattca
     6181 tactgaagat agttacgata gctctattaa aacaattgat ttgatagata gggctattca
     6241 attaaaatat gatgaaatag ctattacaga acatctagat ttgctaccta ttgaaatgaa
     6301 aagctatgga gcaccttcct tgaaacatta tttcaaaaga attaatgatt tacaaaatac
     6361 aatatcagaa ctcacattac ttagtggtgt tgaagtaggt gattatcata tagttaagga
     6421 ttatgctgtt tcacttttag agggtataaa ttttgatttg atattgggat ctgtgcactt
     6481 tctatcggat caaacgaatg tagctattcc tttaaagaaa cagctttccg aagaacagat
     6541 tagagattat tatttgcaaa acttggcttt agtatccaat tgcgatattg atgtccttgc
     6601 ccatttaggg gtttataaac gttgttataa cagtattccg gatgaacgaa ttcactatcc
     6661 gataatacaa gatattttca gggttattat agaccgaaaa atagctctgg aaatcaatta
     6721 cagttcattg cgtagaggat atccttcttt tattccggag cagcatattt tagaattata
     6781 tctagcttta ggaggacaac ttttttcttt aggtagcgat gcacatttca ttgagcattt
     6841 tgatttgttt agagatagca ttccgcaaga attcagcaat tcgtttcgat atcacttata
     6901 gtaaacatag ataacattta tcaatatcct gaatttatcc tgctgtacta aatgtatact
     6961 gtgcttcact atatttaatc tgctttgcta ttttccattt tttgtacttt ctttaggcat
     7021 aaatttccct tgccaaaaat gctatattga ttataatgtc ttaaaaataa acctgcgatg
     7081 agagataagt tctggcatca agggtggtaa aaacctgttt gatttgcctt tttatcatct
     7141 ctcgctgaag gaaattatat tatgaacgag agatgtcata tcctcactat tattatggaa
     7201 gaccgtgaat cagctttcca tccggtaaat gaactcttgc atagttatgc tccacatata
     7261 ttattaaggg ttggatatcc tatgcgcgat ttgggtgttg ctgtaatctt tctaattgta
     7321 gagctaccga tcgctgaaat gggttctttt tctggaaaat taggacagat taagtccgtg
     7381 aaagtacagg ttacaacttt aaagcttgaa aaaggagaaa aaaatgaaca ttagcacaga
     7441 aggcgtaaaa tcctttattc ctacaaccga aattgaagaa catctggcaa ggaatcgttt
     7501 cgctgatacg attcggataa gagatattat tgataaatca ttagataaac agcgtttaaa
     7561 tccagatgag acggctgccc taattaatgt taccgatcca gaattaaagc aacagatttt
     7621 agaaggcgca catactctta aagaacgcat ttacggtaat cgtatcgttt tgtttgctcc
     7681 cttatatgta ggtaacgatt gtataaacga ttgtgtttat tgtggatttc gcagttccaa
     7741 taaagaatgt catcgagcta cacttagtaa agaagaactt atttccgaga ccgaagcttt
     7801 agaagatgca gggcataagc gtttaattat ggtttatggt gaacatccga aatattcacc
     7861 ggagtttatt gcagacacag ttcaaacggt ttataatgtt aaacataaca aaggtgaaat
     7921 tcgcagggta aatattaatg ctgctcccct tgatattgaa ggttttagaa tagtgaaatc
     7981 cgtaggtatt ggaacttatc agatttttca agagacctat catgaagaaa cctataaaac
     8041 tctacatcca aggggaccta aaagtaattt cttatggcgt ttggatagtt tagacagagc
     8101 tatgcaggct ggaattgatg atttaggtat tggtgcttta atgggtttat atgattggca
     8161 ttttgaagtg atggg
//
LOCUS       DAORHB010000047         8167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_8343_length_8167_cov_5.217209, whole genome shotgun sequence.
ACCESSION   DAORHB010000047 DAORHB010000000
VERSION     DAORHB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8343_length_8167_cov_5.217209"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..800)
                     /locus_tag="PKM80_02470"
     CDS             complement(222..800)
                     /locus_tag="PKM80_02470"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNV92801.1"
                     /translation="MKIINKITMFLAKKRLLITRVLCIAVILEFILNFHYRIEHIITN
                     PTAIIISIILIVLGAFIRSWSAGIIEKRNRLVMEGPYALCRNPLYAGSSLILLGAVLF
                     VNDIWAWLLAGLLILVIFPFTIKKEELTLSTNFPEDWKNYTATTGRYFPKKLDWQRLK
                     QKWSFQLWLKNREYNMFLFSLLLIIIICVLGF"
     gene            complement(941..2212)
                     /locus_tag="PKM80_02475"
     CDS             complement(941..2212)
                     /locus_tag="PKM80_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV92802.1"
                     /translation="MNIKDGINSAFQSIFSHKLRSLLTLTGIVIGVLAVVTMFSSVYA
                     IKGLIKKNMEGMGWDNSIIIYPSVGNSDKEIGKNRSHIRRPKQSVPFLNYDDYLALKE
                     NLNYKCIYGSISKQSLYRVGNKVNNIILRATEVDFFKNKSYPIGKGRYFNAYEAENNI
                     PVAVLGYQFAEEYFKDKDPIGQVLALGSHRFTVVGVLAPDVLNSGNGMNFNPWERQWD
                     LKAVYIPLKYGATYIEPNKIIHQIYLQSYSTEDYLKLKNEARQIMLARHNMYPNFQFM
                     DIGDMILKITSEINKFMDKWNITLIAIASISLIVGGIGLFSTLLISIQERMTEIGIRK
                     SIGATEQDIFFYFIFEALALAFTGAVIGVILAWILITLIASAIKFPLYLPMQGVVVGI
                     GFSLLIGFLSGLYPAWKATGIDPIQAIYYRE"
     gene            complement(2216..4435)
                     /gene="topA"
                     /locus_tag="PKM80_02480"
     CDS             complement(2216..4435)
                     /gene="topA"
                     /locus_tag="PKM80_02480"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNV92803.1"
                     /translation="MSKGLIIVESPAKAGTISKFLKNKYNVKASMGHIRDLPKHELGV
                     SVSQNFKPDYIMDKSKSKVISELKEATLAADSVFLASDNDREGEAIAWHLTEALKKEL
                     QNKPVYRIVFNEITSQAINEAIKNPGKIDMAKVEAQQARRILDRLVGYEISPLLWKVI
                     TKELSAGRVQSVALRLICEREAEIKAFVPKEFWKVEGDFWKDNLPPFKAVLEKINGKK
                     VEIPDEKSAIEIVDSIQNAETKLKEIKRSNRNIDPLPPFITSTLQQEASKILGFQAQK
                     TMSIAQVLYEGIDLGGESTGLITYMRTDSTRMAEEAVTKAKDLIKERFGADLVNPKVR
                     VFKNKQSAQDAHEAIRPTDPFRTPESIAQYLTKEQLKLYTLIWQRFIATQMKPVKLLS
                     TNVKISMGNAEFTSTGAQIIEEGFLKAYSYINIPLGEKIHPDYTKDDELQHNALEKTQ
                     HFTTPPSRFTEASLIKELEAKGIGRPSTYASIIDTLRKRKYVNMEKKSFIPTPLGNDV
                     NRFLVDKFDCLFNVKFTAEMETKLDEVEYSKIAWNDVVKEYYDQLQALINQVDIKKEK
                     NNFIQDTGIACDVCKQGTMLIKHSKRGEFLACSRYPQCKNSKSFTRDADGNIKIVVPT
                     PLEEKCPECGSPLMLRNGKYGEFIACSNYPKCKYSRPKTLGIKCPECGIGELTAHKSK
                     KGRPFYSCNRYPECKYITNDKPLPISCPKCGNPYLVEKYSREKGKYKMCPKCKTEME"
     gene            complement(4473..7634)
                     /gene="secA"
                     /locus_tag="PKM80_02485"
     CDS             complement(4473..7634)
                     /gene="secA"
                     /locus_tag="PKM80_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNV92804.1"
                     /translation="MLEKVLKKIFGDKNIQDLKHYEPVVREINEIYSGLEQYEDEQLI
                     NRIQEIKQEIADKLNPLRNKLSDLEKEYRETREDNERNRLDNSIDTTKKELKLVTKNT
                     VDDYLPEVFAIVKDTCRRMLGQKFEVRGHEVEWNMVPFDVQLIGAMALHDGKIAEMAT
                     GEGKTLVATMPLFLNALVGRGVHLVTVNDYLASRDAEWMSPIFNFHGLSVGCITTGMD
                     FDSRKEAYNCDVTYGMNSEFGFDYLRDNMAVSPEQLVQRDFYYAIVDEVDSILIDEAR
                     TPLIISGPIAQDKNFYRELRPLIAQVVQTQNALVQRYLSEINEDLNDNNPNADNNRLA
                     KNLLLLKRAAPRNKAFMKLMQDGELKKMVNDIEGIYLRDKKMNELDDNLFFVVEERHN
                     SVDLCEKGREMLSKKDKDLFVVQSLDEILAEIDNNEALSEEEKQQQKAIQTNRFMDKS
                     EKLHNINQLLRAFTLFENNQEYVVIDNKVIIVDEFTGRQMPGRRFSDGLHQALEAKEN
                     VEIEAGTQTFATITLQNYFRMYDKLSGMTGTAVTEESEFMEIYNLPVMVIPTNVPITR
                     IDHDDLIYLSKNDKYQAIINEIIYWHEKQKPVLVGTVSVEVSEIISRLLKRKGITHNV
                     LNARQHQREAEIITEAGQPGAVTIATNMAGRGTDIKLGKGVVEGPYESYVGLPKTLTE
                     EYPYGLPLDGLHVIGSERHESRRIDRQLRGRAGRQGDPGTSRFYLSLEDDLMRLFGSD
                     RIAPMMVKMGLKSGDVIRHPWMTKAVEKAQKRVEEHNFEIRRELLKYDEVMNQQREVI
                     YSYRRSVLKGYDLKNEILEMITDTIVNMLDEYIPPHSYPEDWNLERICQWFQRNLNLG
                     LNVKDIFSDHLNRELLENTLLEYALSAYENKERQIGSEQLRNIERRALLEVVDDEWRD
                     HLHEMDLLKDGVYLRAYANKDPLIEYKKESYDLFQGLIARIQENVSRKVFTTYVLSRE
                     QMENFLKNANLTHQDLNAFLKAQQEQQMNQNMTAPPQYNNPGGEIEKVRPVKVAPKVG
                     RNDPCPCGSGKKYKKCCGMYDTE"
BASE COUNT         2397 a         1685 c         1314 g         2771 t
ORIGIN      
        1 caaattttgt agattacata aataatatag tacacaggga taattgttgc ttaatgaaat
       61 acagtatata tgtagtaact tctcggggaa ttttgccgag aggcataacc gtatactttc
      121 gataaaaatt aagtaagaaa caaactcgcc cctgaaaagg aagttgcctc agctatctat
      181 ttacaaaatt atgcgtaaat tcccttttcc ccacttcctt tttagaaacc aagaacgcat
      241 ataatgatta tcagcagaag cgaaaacaga aacatattat actctctatt tttaagccac
      301 agttgaaacg accatttttg cttcaaacgc tgccaatcta attttttagg aaaatatctt
      361 ccagttgttg ctgtataatt cttccaatcc tcagggaaat ttgtacttaa ggttaattct
      421 tcctttttaa ttgtaaacgg aaagataacc aaaatcaaca gaccagctag caaccaagcc
      481 caaatatcat tcacaaataa aacagctccc aataaaatta aagaactgcc tgcatagaga
      541 ggatttctac ataaagcata aggtccttcc attacaagcc gatttctttt ttcaattatt
      601 ccagcagacc aagaacggat aaaagcaccc aaaacaatca gaattatact gattatgatt
      661 gcggtaggat ttgttattat atgttcaatg cgatagtgaa agttaagaat gaattccaaa
      721 ataacggcta tgcagagaac tctggtaatt agcaatcgct tttttgccaa gaacattgtt
      781 attttgttga ttattttcac tcggatttct cctttttaat atttgcggag gaacggcgtc
      841 ctccggctac acatttttca tattttgcag aggaacagct cctccggcta cccaaaaata
      901 agttcggaga aaaaaacgac aagttgattc ttccctattt ttattctcga tagtaaattg
      961 cttggatagg atcaattcca gttgctttcc aagcaggata aagtccagat agaaaaccta
     1021 tcaacagaga gaaaccaatt cctacaacaa ctccttgcat aggcagataa agaggaaatt
     1081 ttatagcgct tgctattagc gtaatcaata tccaggcaag gataactcct ataacagctc
     1141 ctgtaaaagc caaagccagt gcttcaaaaa taaaatagaa aaagatatcc tgttctgtag
     1201 ctccaatgct tttgcgaatg ccaatttcag tcattctttc ttgaatgctg atgagtaaag
     1261 tgctaaatag ccctattcct cctacaatta aagaaatgga agcaatggca ataagagtta
     1321 tattccactt atccataaat ttattaatct ccgaagtgat tttcaggatc atatcgccaa
     1381 tatccataaa ctgaaaatta ggatacatat tatgtctggc aagcattatc tgtctagctt
     1441 cattttttaa tttgagataa tcctcggtgg aatatgattg tagatagatc tggtggataa
     1501 tcttattggg ctctatgtaa gtagctccat atttcaaagg tatgtataca gctttcagat
     1561 cccactgcct ttcccagggg ttaaaattca ttccattgcc tgaattcaat acatcaggag
     1621 ctaaaacacc cacaactgtg aaacgatgcg aacccagagc taaaacctgt ccaatagggt
     1681 ctttatcttt aaaatattct tcggcaaatt gataacccaa aacagcaacc ggaatattgt
     1741 tttctgcttc gtaggcatta aaatagcgtc ccttgccaat aggataactt ttatttttaa
     1801 aaaaatctac ttccgtagca cgcaggataa tgttatttac tttattccca acccggtata
     1861 agctctgttt ggaaattgac ccgtaaatac atttataatt caggttttct ttcagagcca
     1921 gataatcatc ataatttaga aagggtacgc tttgtttagg tcgccttata tgactgcggt
     1981 ttttccctat ttctttatca ctattgccta cacttgggta aataatgatg gaattatccc
     2041 agcccatacc ttccatattt tttttaatta gtcctttaat ggcataaaca ctggaaaaca
     2101 ttgttacgac ggcaagcaca ccaataacta taccggtaag agttaaaaga gacctaagtt
     2161 tatggctgaa aatgctttgg aaagcactat tgatgccatc ttttatgttc attgcctatt
     2221 ccatctcggt tttgcatttg ggacacattt tatatttacc tttttcgcgg ctgtattttt
     2281 ccaccaaata aggatttcca cacttaggac agctgattgg aagtggttta tcgttagtta
     2341 tatatttaca ttcaggatag cgattacagg aataaaaagg acgtcctttt tttgatttgt
     2401 gggctgttaa ttctccaata ccacattccg gacacttaat tcccaaagtt ttaggccttg
     2461 aatatttaca cttaggataa ttggaacaag cgataaattc tccatatttc ccgtttctaa
     2521 gcattaaagg cgaaccacat tcagggcatt tttcctctaa aggagtagga acaacaattt
     2581 ttatatttcc atctgcatcg cgggtaaagc ttttgctgtt tttgcattgg ggatagcggc
     2641 tacaggcaag aaattctccc cttttactat gtttgattag catagttccc tgtttacaaa
     2701 catcgcaagc aatgcctgta tcctgaataa agttgttttt ttccttttta atatctacct
     2761 gattgatcaa tgcttgtaat tgatcataat attccttcac tacatcattc caagcaattt
     2821 tactgtattc cacttcatcc agtttggttt ccatttcagc ggtgaatttt acattgaaga
     2881 ggcaatcgaa tttatctacc aaaaaacgat taacatcgtt ccccagggga gttgggataa
     2941 atgacttttt ctccatattt acatattttc gctttctaag agtgtcaatt atggaagcat
     3001 aagtagaggg acgtccaatc ccttttgctt ccagttcttt gatcaaagat gcttctgtaa
     3061 atctggaagg aggagtagta aaatgttgag tcttttccaa cgcattatgc tgtaactcat
     3121 catctttggt ataatcagga tgaatctttt cccccaacgg gatatttatg tatgagtatg
     3181 ctttcagaaa accttcttca ataatttgag ctccggtaga agtaaattca gcattgccca
     3241 tactaatttt tacattggta gaaagcaatt ttacaggttt catctgagtg gcaataaaac
     3301 gctgccagat aagagtatat aatttcagtt gttctttggt taaatactgg gcaatgcttt
     3361 ccggagttct aaaaggatca gtagggcgaa ttgcttcatg agcatcttga gcactttgct
     3421 tatttttaaa aacccgcacc ttaggattaa ctaaatcggc accaaacctt tctttaatca
     3481 ggtctttagc cttagttaca gcttcttcag ccattctggt agaatcggtt cgcatataag
     3541 ttatcagacc agtactttct cctcccaaat caataccttc ataaagaact tgagctatgc
     3601 tcatcgtttt ttgtgcctga aaaccaagta ttttacttgc ttcttgttgt agagtactgg
     3661 ttataaaagg aggaagagga tcaatattac ggttactgcg ttttatttcc tttagttttg
     3721 tttctgcatt ttggatacta tccacaattt ctatggcact tttctcatcg ggaatttcca
     3781 cttttttacc attgattttt tctaaaacag ctttgaaagg tggcaaatta tctttccaaa
     3841 aatccccttc aactttccaa aattctttag gaacaaatgc cttgatctcg gcttcccttt
     3901 cgcagattaa tcgcaaagca acagattgaa ctcttccagc agaaagctct ttagttatca
     3961 ctttccaaag cagagggctt atttcataac caaccagacg gtctaaaatt cttcttgcct
     4021 gttgagcttc cacttttgcc atatctattt tgccggggtt tttaattgct tcattaattg
     4081 cctgtgaagt tatttcatta aacacaatcc gataaacagg tttattttgc agttctttct
     4141 ttagtgcttc tgttaaatgc caagctatag cttcaccttc acgatcatta tcacttgcca
     4201 gaaaaacact atccgcagcc aaagttgcct cttttaattc agagataact ttgctcttgg
     4261 atttatccat tatataatca ggtttaaaat tctgcgaaac actcactccc aattcatgct
     4321 ttggcagatc acggatgtgt cccatcgatg cttttacatt atatttattc ttcagaaatt
     4381 tgctgattgt tcctgctttt gcaggagatt ctactataat tagtcctttg ctcatatctt
     4441 tcctttttac caattaacgc ttactatctc acttattctg tatcatacat tccgcagcat
     4501 ttcttatatt tttttccgct tccacaaggg cagggatcat ttcttccaac tttgggagct
     4561 actttaacag ggcggacttt ttctatttcg ccacctggat tattatattg agggggggca
     4621 gtcatatttt gattcatctg ctgttcttgc tgtgctttta aaaaagcatt caagtcttga
     4681 tgagttaagt tcgcattctt caaaaagttt tccatttgtt ctcgagataa cacataagta
     4741 gtaaatactt ttcgggaaac attttcctgg atgcgggcaa ttaaaccttg aaacaggtca
     4801 taactctctt ttttatattc aattaatgga tccttattcg cgtaggcccg taaatataca
     4861 ccatctttca gaaggtccat ttcatgtaaa tgatcgcgcc attcatcatc tacaacttcc
     4921 aaaagagctc gtctttcaat atttctaagt tgttctgatc caatctgtct ttccttattt
     4981 tcataagcgg aaagcgcata ttccaaaagg gtattttcca gcagctctct atttaagtgg
     5041 tcgctaaata tatctttaac attaagaccc agatttaaat ttcgctgaaa ccactgacag
     5101 atgcgttcca gattccaatc ctcaggataa ctatgaggtg gaatatattc gtctagcata
     5161 tttacgatag tatctgtgat catttccaaa atttcatttt tcaggtcata acccttcaac
     5221 acactacgac gataagaata aataacttcg cgttgctgat tcattacttc gtcatattta
     5281 agcagttctc tccggatttc aaagttatgt tcttccactc ttttttgagc tttttccact
     5341 gctttagtca tccacggatg acgaattaca tctccagatt ttaaacccat tttcaccatc
     5401 atcggagcta tcctatcaga accaaaaaga cgcattaaat catcttccag agaaagataa
     5461 aatcgggaag ttccaggatc accttgccgg ccagctcttc ctcgaagttg tctatcaata
     5521 cgtctacttt catgtctttc actaccaatt acatgaagtc catccaaggg taggccatag
     5581 ggatattcct ccgttaaggt tttcggtaaa cccacatagc tttcatatgg accttccaca
     5641 actcctttgc caagctttat atcagtacct cgaccagcca tattagtagc gatagttaca
     5701 gctccaggtt gaccggcttc tgtaattatt tctgcttctc tttgatgttg acgagcattt
     5761 agtacattat gagttattcc tttccgtttc agcaaccggg aaataatttc ggagacctct
     5821 acgctcaccg ttccgaccaa gacaggcttt tgcttttcat gccaatagat tatttcattg
     5881 ataattgcct gatatttgtc atttttgctt aggtaaatca gatcatcatg gtcgattcgc
     5941 gtaataggta cattggttgg aatcaccatc actggcagat tgtaaatttc cataaactct
     6001 gattcttcgg taacagcagt tcctgtcatt ccggataatt tatcatacat tctaaagtag
     6061 ttttgtaaag taatagtagc aaaggtttgc gtgccagcct caatttccac attttctttt
     6121 gcttccagag cttgatgcaa gccatcggaa aaacgacgtc ccggcatttg tctaccagta
     6181 aattcatcta ctattattac cttgttatca ataaccacat attcctggtt attttcaaag
     6241 agggtaaaag ctcttagcag ctgattgata ttatgcaatt tctcactttt atccataaag
     6301 cgattggttt gaattgcttt ctgctgttgt ttttcttctt cagaaagggc ttcattgtta
     6361 tctatctcag ccagaatttc atcgagggat tggaccacga agaggtcttt atcttttttg
     6421 gaaagcattt cgcgcccttt ttcacacagg tcaacgctgt tatgtctttc ttccaccaca
     6481 aagaaaagat tatcgtccaa ttcattcatc tttttatccc taagatagat gccctctatg
     6541 tcattcacca tttttttcag ctcaccatcc tgcatcaatt tcataaatgc tttatttcga
     6601 ggagctgctc gtttcaataa aagaaggttt tttgctaagc gattgttgtc agcattagga
     6661 ttattatcat ttagatcttc attaatttcg cttaaatacc tttgcactaa agcattttgc
     6721 gtttgaacca cttgagcaat taaaggacgt aattctctat aaaaattctt atcctgagca
     6781 ataggaccac taattatcaa aggtgttctg gcttcatcaa ttaaaatgct atccacttcg
     6841 tccacaatag cataataaaa atcacgctga accaactgtt caggagaaac agccatatta
     6901 tcacgcaaat aatcaaaacc aaattcactg ttcatcccgt aagttacatc acaattatag
     6961 gcctctttac ggctatcaaa atccattcca gtagttatac aacctacaga aagaccgtga
     7021 aaattaaaaa tagggctcat ccattcagca tcacgacttg ctaaataatc atttactgta
     7081 actaaatgga caccgcgtcc aactaaggca ttcaaaaata agggcatagt agccacgagg
     7141 gttttacctt ccccggttgc catctcagca attttcccat catgcaaggc catcgcacct
     7201 ataagctgta catcaaaagg caccatattc cattcaactt catggccgcg aacttcaaat
     7261 ttttgcccaa gcatacgacg acaagtatct tttacaatag caaaaacctc tggcaggtaa
     7321 tcatccactg tattttttgt tacaagcttc agttcctttt ttgtcgtatc tatagaattg
     7381 tctaatcggt tgcgttcatt atcttccctt gtttcacgat actccttttc cagatcggag
     7441 agcttattac gcaggggatt taatttatca gctatttcct gtttgatctc ttgaatgcga
     7501 ttgataagct gttcatcttc atattgttca aggcctgaat atatctcatt tatctcccga
     7561 acaacgggtt catagtgttt caggtcttga atgtttttat cgccaaatat cttttttagt
     7621 actttctcta acattttgtt tccttgttac tcggtttaca aatagctctg caagccaagg
     7681 ttaatgcatt agtccctttt ttgtcagttt attttgaagg tggaattgtg tcaaataaaa
     7741 aaggatacac tgtttagatt tgttccacac agatttcgct ttctcacaca tattatgcag
     7801 atttatatgc gttcacattt gtttttcaac tcgttttacc atttttccat ttaagcaact
     7861 aatctttctc aggagcccaa gtgagtgaga gacaaaaaaa atagcataaa atactactta
     7921 gtatgaacac ctcgtatatt attgttgcat taacaattat acaactaaca ataatgaggt
     7981 atcaaaacga attatctgag aattgtcaat accatctcca gtttgtgcat cgggtatagg
     8041 tatgtcattt ccttcaaact tataagtcat agtgtcagga attgctgtta catcaatcgt
     8101 aaacactgaa tctataacag catttgatcc tgtagatttc cattcaatgg aatatgcagc
     8161 gatgctg
//
LOCUS       DAORHB010000048         8149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_8372_length_8149_cov_4.453546, whole genome shotgun sequence.
ACCESSION   DAORHB010000048 DAORHB010000000
VERSION     DAORHB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8372_length_8149_cov_4.453546"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKM80_02490"
     CDS             complement(<1..291)
                     /locus_tag="PKM80_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNV92805.1"
                     /translation="MSSTPRGERLIIAIIGKRNAGKSSLINALVGQEIAIVSEIPGTT
                     TDPVDKHYELLPLGPVTFYDTAGVDDTGELGQKRVQATQKILYRADIAIFVSD"
     gene            complement(525..1175)
                     /gene="coaE"
                     /locus_tag="PKM80_02495"
     CDS             complement(525..1175)
                     /gene="coaE"
                     /locus_tag="PKM80_02495"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424716.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNV92806.1"
                     /translation="MQLNNRPLLIGITGNIGSGKSSFCNYLAANGLRIISADVLANQH
                     LEDKDIQSALVTRYSPDILTTSVRDKNVLTINRKLLADKVFISETETNYLNSLIHPLV
                     LQDFQQIAEQSQEKVLCFEVPLLFEANLQDCFDYLILISASKEKRLDRLEKKGEEKSK
                     AEKRIMHQIPDINKRNLVDLVIENNNDIDALHKAADNFANHISEIKLKEVRPFILV"
     gene            complement(1162..1908)
                     /gene="cdaA"
                     /locus_tag="PKM80_02500"
     CDS             complement(1162..1908)
                     /gene="cdaA"
                     /locus_tag="PKM80_02500"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNV92807.1"
                     /translation="MEFLIPRYQDIVDILFIAFLIYQALLIVRKSGGYQVLWGLLFVL
                     VLYIVAIIFNLKMVGSLLNAIRNYWFIAVVILFQPELRSILSHLNLPRELSKAFSKQE
                     KSSLYTPIIDAVSSMSFRKIGALIVLENKRKLNEYIYSGEPIDAAVSMRLILTIFNPK
                     SVLHDGAIIIRDQRIMAAKVVLPLSRKDEYVRKFGTRHLAGIGITEISDAIAIIVSEQ
                     TGQISVANAGKIQTNVAFEELMQILTDATK"
     gene            complement(1909..2742)
                     /gene="folP"
                     /locus_tag="PKM80_02505"
     CDS             complement(1909..2742)
                     /gene="folP"
                     /locus_tag="PKM80_02505"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNV92808.1"
                     /translation="MNKNYYPEHIPVIMGILNVTEDSFSDGEQFLDRDSAIKHSIEMI
                     AQGAEIIDIGAESTRPGSLPVNSETQLKRIVPIITTIKEKYPDQILSVDTQNTAVAKK
                     SIELGVTIINDISALRTDSEMAELLADNPQIKVVLMHMQGIPRTMQLNPFYTDVIAEI
                     NDFFQERIDYCLSKGILLENIILDPGIGFGKTLEHNLTILANLKSFKQFGLPVLLGAS
                     RKSFIDKISPSLPSQRIGGCLAAAFAAACGEMDIIRVHDVLAHKQFFDVLIAIAKAGK
                     N"
     gene            complement(2735..3454)
                     /locus_tag="PKM80_02510"
     CDS             complement(2735..3454)
                     /locus_tag="PKM80_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92809.1"
                     /translation="MRRFNPFKLFNGYFISVVFHLLLFLFFAFYFLKPYTPEKWYSFE
                     WESSQENKVKKTSSLRIEDLIGGKNAAGSAGISKSQESSGNMSLNLNKLAPERAGESE
                     SVITMPLNVPNQEENKANIPSSFTRNRGAHSLKEIPGTFQKTNPNFSATLEEGGGEAY
                     IISQSKPQIIPTEEGEVYIEFKLMRNGAIDMNSVNILSFTNAAYVESIRKVMPSWKFG
                     FKGVYNPKTTYRLLCRFVINE"
     gene            complement(3457..3858)
                     /locus_tag="PKM80_02515"
     CDS             complement(3457..3858)
                     /locus_tag="PKM80_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNV92810.1"
                     /translation="MKLRVSKQRISTTMLISMTDVIFLLLLFLLIGSNFVSQTGLPIR
                     LPGSSTSVRQSYETLLITYYNDGHLYFMDRPITIQQLKDILQQKYQTPEQVVRIAVEK
                     QVSVQKLIDLMDIVREIGYEHIFIATTVEKG"
     gene            complement(3855..4481)
                     /locus_tag="PKM80_02520"
     CDS             complement(3855..4481)
                     /locus_tag="PKM80_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNV92811.1"
                     /translation="MSILDLIVKGGFLMYVLTVISVISIGIIIEKYRQFRKVRIANNK
                     LIESLKSQDKLENIRALINLHQNECPMGIMLYKLFNSPSDDLDLINQSMEATANLELH
                     KLERGLGWLSTFAAIAPLIGFLGTVLGMVNVFMKIQAANQSGVDISMLAGGIWVALLT
                     TVGGLIVGIFTIIFYNDLVQKLENIVKDMQDNAIEHIIKYKKIMQGKL"
     gene            complement(4578..5663)
                     /gene="serC"
                     /locus_tag="PKM80_02525"
     CDS             complement(4578..5663)
                     /gene="serC"
                     /locus_tag="PKM80_02525"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNV92812.1"
                     /translation="MERVHNFNAGPAVLPEEVLREAQSDLFNYKGMGLSVMEMSHRSK
                     EYEAIINEARNAAKRIYGLGDDWDVLFLHGGASLQFLMVPLNFIPEGKVANYIHTGVW
                     SKKAMAEVKKLNKPLHIAASSEDKSFSYIPKNYQLSDNPAYLHITTNNTIYGTEWKTD
                     PDVPEGIPLIADMSSNFMSKPIDINKYSLIYAGAQKNVGPAGCAVVLVKKDFLATAAA
                     NLPSMLDYQIHAKNGSMYNTPPCFTIYMVGLVMKWIENYGGLAMIEQNNKAKAKYIYD
                     AIDASNGFYRGTVIPEYRSLMNITFRLPNEELEALFISEAKKNGMIGLKGHRDVGGCR
                     ASCYNALPLNAAYDLAEFMKNFQKQNG"
     gene            5805..6398
                     /locus_tag="PKM80_02530"
     CDS             5805..6398
                     /locus_tag="PKM80_02530"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNV92813.1"
                     /translation="MIHELLHNKKVILASASPRRKELFALLGINAYIIPAEIAEPING
                     EEPQVQAMQNALRKAEIVKNKVDKDAVVIAADTLVAIDNHILGKPLNQKEASRYLRLL
                     SGNTHSVYTGICIFYNEIANINYEQTLVHFAELTELEIKSYIETGEPMDKAGAYGIQG
                     YGAQFITAVDGCYFNVMGFPVRKFYEMLNAILEKEEK"
     gene            6395..7858
                     /locus_tag="PKM80_02535"
     CDS             6395..7858
                     /locus_tag="PKM80_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV92814.1"
                     /translation="MNILANLYLWIEGKFTEEPKSLLVHNTSISEILPYPVPEQYKNI
                     SMKDLNGSYAYPGFIDTHTHSFEGGLYSLGVDLSTAKNIEDILALLSAANSKKTKDEF
                     IFAWQMDETLLKERRFPTLTELNSAVPDKPLILRRIDGHSCMLNSYARNMVTGLKTKE
                     EILRGGENDYAVHFFHKRLNDEAILNAYRAAANIGLQAGFTSLHTMIGDAENSINHYE
                     LIRDNLNKFPLRFILYPQSFNIKAALEAGANRIGGCILADGSIGSETAAISEPYLNSN
                     SWGVLYQSDEFWQKFITEASQHRLQVAIHCIGDRAIRQINNVYKSLASTADLRHQLIH
                     CEITPDNLIEEIATSGAVPVMQPNFDYLWGGENGFYAKKIGIKRSRIMNRFATLLKSG
                     ITITGGSDWYITSLNAAHSLRAAINHHNPLERLSTAQAIDIYTKNAAWLNSEEDTYGK
                     IKIGYKADLSIYSHPIENETTELYYLIRDGEIKYAAI"
     gene            7845..>8149
                     /locus_tag="PKM80_02540"
     CDS             7845..>8149
                     /locus_tag="PKM80_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92815.1"
                     /translation="MQLSDNDLIKVDIKQVIKQELLFHPELQLIDLYKLVFQAYMGPG
                     HLINNIDEAANYIITEMLLHSEPYLPIKQDIGNGKGFFRVSLDLLMPEKELKKEQAIV
                     "
BASE COUNT         2611 a         1477 c         1454 g         2607 t
ORIGIN      
        1 atcagaaaca aaaatagcga tatcagcacg atatagtatt ttttgagtgg cctgaacgcg
       61 tttttgtccc aattcacctg tatcatcaac tccagcggta tcataaaaag taacgggtcc
      121 caaaggtaga agttcataat gcttatctac gggatcagta gttgtgcctg ggatttcgga
      181 cacaatagct atttcctgcc ccacaagagc atttattaaa ctgcttttac cggcatttcg
      241 ttttccgata atggctataa ttaagcgttc accgcgaggc gtactgctca tttttttact
      301 ccatattcta atatacctat tccttttctt aagaacttag gtattagtca agtcctttct
      361 ttgcttatta ctttaggaag tagaaaagtt attgatgtca gcaagtttaa ttaatgcaaa
      421 ggttatttta cctgtatata tagtgattag caaaaaacat caaagctgat aggaatgtaa
      481 actacgcaaa atgtaatggg aattggataa gtgctggcaa caaattatac taaaataaag
      541 ggacgcacct cttttagttt aatttcgcta atatggttgg caaagttatc tgcagcctta
      601 tgaagggcat ctatatcatt gttgttttct ataactaagt ctaccaaatt gcgtttatta
      661 atatccggta tttggtgcat aattcttttt tctgccttcg atttttcttc accttttttt
      721 tctaaacggt ctaaacgttt ttctttagaa gcactaatca aaataagata atcaaaacaa
      781 tcttgaagat ttgcttcaaa aagaagagga acttcaaagc agagaacttt ttcttggctt
      841 tgttcagcta tttgctgaaa gtcctgtaag acaagagggt ggattaagga gtttaaataa
      901 ttcgtttctg tttcactaat aaaaaccttg tcagccaaga gtttacggtt tatagtaaga
      961 acatttttat ctcttacgga agtggttaaa atgtctggag aatagcgtgt taccaaagca
     1021 ctttgaatgt ctttatcttc cagatgttgg tttgccagaa catctgcaga gattattctc
     1081 aagccattag cagccaggta attacagaaa gaactctttc cgctaccaat atttcctgta
     1141 attccaataa gcaaaggacg attatttagt tgcatcggtt aaaatctgca tcaattcttc
     1201 aaaagcgaca ttcgtttgaa tttttccagc gttagctaca gatatttgtc cagtttgttc
     1261 agaaacaata atagctattg catcgcttat ttctgtaatt cctattccgg ctaaatggcg
     1321 agtaccgaat ttacgaacat attcatcttt tctcgataga ggtagcacta ctttagcagc
     1381 cattatacgc tggtcgcgaa taataattgc tccatcatga agcacgcttt taggattgaa
     1441 tattgtaaga ataagccgca ttgaaacagc ggcatcaata ggttcgccgc tatatatata
     1501 ttcatttaat ttccgtttat tttccagcac aatcaaagct ccgattttac gaaaactcat
     1561 agaagaaaca gcatcaataa taggagtata gagggaggat ttttcctgtt tactgaatgc
     1621 tttacttaat tctcgcggta gatttaaatg agataaaatt gaacgtagtt ctggttgaaa
     1681 gagaatcaca actgctataa accaatagtt acggatagcg ttcagcaatg acccaaccat
     1741 cttaagattg aatattatcg ctacaatata gaggacaaga acaaacagta aaccccataa
     1801 aacctgataa cctccggact tacgaacaat tagcaatgct tgatatatca ggaaagcaat
     1861 aaacagtatg tctacaatgt cttgataacg aggaattaaa aattccactt aattttttcc
     1921 agccttggca attgctatta aaacatcaaa gaattgttta tgagccagca catcatgcac
     1981 ccgaataata tccatttctc cacaggcagc agcaaaagca gcggcaagac atcctcctat
     2041 gcgttgcgag ggaagtgagg gagaaatttt atcaataaag ctttttctgc tagcacccaa
     2101 cagaacaggt aatccaaatt gtttgaagga tttcaaattg gcaaggatgg ttaaattatg
     2161 ctccagggtt tttccgaaac caattccagg atcaagtatt atattctcga gcagaattcc
     2221 tttacttaaa caataatcta tccgttcctg aaaaaagtca ttgatttctg ctatcacatc
     2281 tgtataaaaa ggattgagtt gcattgtgcg tggaattcct tgcatatgca taagaacgac
     2341 tttaatttgc ggattgtcag ctaacaattc agccatctca gaatcggttc tgagagcaga
     2401 aatgtcgttg attatagtga cgccaagttc tatggacttc tttgctacag cagtattttg
     2461 agtatctacc gatagaattt gatctggata cttttctttg atagtagtaa taattggaac
     2521 gattcttttc aattgagttt ccgagtttac aggcaaagaa ccggggcggg tagattctgc
     2581 tccaatatca attatttctg ctccctgagc aatcatttct attgaatgtt taattgcgga
     2641 atccctatct aaaaactgtt ctccatccga aaatgaatcc tcggtaacat tcaagatacc
     2701 cattataaca ggaatatgtt caggatagta attcttattc atttatcaca aacctgcaaa
     2761 gtaaacgata agtagtttta ggattgtata cacctttaaa tccgaacttc caggaaggca
     2821 taactttacg gattgattct acataagctg cattagtaaa ggaaagaata ttaacactgt
     2881 tcatatctat cgctccattt ctcataagtt taaattctat ataaacctca ccttcttcgg
     2941 taggaattat ttggggtttg gattggctga taatatatgc ttctccacca ccctcttcta
     3001 aagttgcaga aaagtttgga tttgttttct ggaaagttcc tggtatttct tttaaagaat
     3061 gggctccacg attacgggta aaactggaag gaatattagc tttattttct tcttgattgg
     3121 gaacatttaa aggcatagtg ataacagatt cactttcacc agcgcgctca ggagctaatt
     3181 tgttcagatt taagctcata tttccagagc tttcctggga tttggaaatt ccagctgatc
     3241 ctgcagcatt ttttcctcca atcaggtctt caattcttag ggaagaagtt ttttttactt
     3301 tgttttcttg cgaagattcc cattcaaagc tataccattt ttccggagtg tatggcttaa
     3361 gaaaatagaa ggcaaaaaag aggaataaca gaaggtgaaa aacaacagag ataaaatatc
     3421 cgttaaatag cttgaaagga ttaaagcgac gcactactat cccttttcta ctgtagtggc
     3481 aataaagata tgttcgtagc cgatttcacg aacaatatcc attaggtcaa ttaatttttg
     3541 cactgacact tgtttttcga cagcaatacg gacgacctgc tcaggagttt gatatttttg
     3601 ctgtaatata tctttaagct gctgaatagt tatgggtcta tccataaaat acagatgtcc
     3661 atcattataa taggtgatta ataaagtttc ataactttgg cgtaccgaag ttgaagaacc
     3721 gggaagccga attggaaggc ctgtttgact aacaaaattc gagccgatta ataaaaacaa
     3781 taatagaagg aagatgacat cggtcatcga tattagcata gttgtggaaa tccgttgttt
     3841 ggaaacccgc agtttcatag ttttccttgc attattttct tgtatttaat gatatgttca
     3901 attgcattat cctgcatatc ttttactatg ttttcaagtt tttggactag gtcgttgtaa
     3961 aaaatgatgg taaaaatacc tacaatcaat cctcctacgg tagttaacag agcaacccag
     4021 attcctcctg caagcataga aatatctact ccactttgat tcgctgcttg gattttcata
     4081 aatacattca ccattcctaa aactgttccc aaaaaaccta tcaagggagc aatagcggca
     4141 aaagtagaaa gccagcccaa acctctttcc aatttatgca gttctaaatt tgctgtagct
     4201 tccatactct gattaattaa atccagatca tcactgggac tattaaacag tttatagagc
     4261 attattccca ttggacattc attctgatgt agattaatta gtgcccggat gttttccaat
     4321 ttatcctggg atttcaatga ttctatcagt ttattattag caattcttac ttttctgaat
     4381 tgacggtatt tttcgataat aattccaatg ctgattacgg agataaccgt aagtacatac
     4441 atcaggaatc cgccttttac gattaaatct aaaattgaca ttaaacctca cgcatgcgtt
     4501 tttgctttta tattactttt actgctgtag taccggttaa ggtactacag cagtagcatt
     4561 actattatag ttaataatta cccattctgt ttttggaagt tcttcataaa ttcagctaaa
     4621 tcgtaagctg cgttcagcgg aagagcgtta taacaggaag cacggcatcc acctacatca
     4681 cgatgacctt tcaggcctat cattccattt ttcttcgctt cactgataaa gagagcttcc
     4741 agttcttcat ttggaaggcg gaaggtgata ttcattaaag aacgatattc aggaatcaca
     4801 gttcctctat aaaaaccgtt tgaagcatca atagcatcgt aaatgtattt tgcctttgcc
     4861 ttgttattct gctcgatcat ggctaatcca ccatagtttt caatccattt catcacgaga
     4921 ccaaccatat aaattgtgaa acaaggagga gtattataca tacttccatt tttagcatgt
     4981 atttgataat ccagcatcga aggaagatta gcggcagctg tagctagaaa atctttttta
     5041 accaaaacaa cagcacaacc tgcaggacca acatttttct gagcaccggc atatattaag
     5101 ctgtatttat taatatcaat cggtttgctc ataaagtttg agctcatatc agcaatcaaa
     5161 ggaatacctt caggaacatc gggatctgtt ttccactctg taccgtaaat agtattatta
     5221 gttgtaatat gcaagtaagc agggttatct gatagctgat agttttttgg aatgtagcta
     5281 aaacttttat cttcggaact tgcagcaata tgcaaaggtt tattcagttt ttttacttct
     5341 gccattgctt tcttactcca tacaccagta tgaatataat tggccacttt cccttccgga
     5401 atgaagttta agggaaccat caagaattgt aaactggctc ctccatgcag aaaaaggaca
     5461 tcccaatcat caccaagacc gtaaatcctc tttgcggcat ttcgtgcttc gttaataatt
     5521 gcttcatact ctttactgcg atggctcatt tccataacgg aaagccccat ccctttgtaa
     5581 ttgaaaaggt ctgactgtgc ctctctaaga acttcttcag gtaagacggc aggacctgca
     5641 ttaaagttat gtactcgttc catttgtatc ctccaggata ttttttttct ttctatggac
     5701 aagtttttct aataccttac tttaaggcaa gttatttttt acaaatagag aaaattattg
     5761 acttaaacca gcctttatat tctatgtaac aaaggtttaa aattatgata catgagttat
     5821 tacacaataa aaaagttatc cttgcttctg cttctccgcg cagaaaagaa ctttttgctt
     5881 tattgggaat aaatgcttat attattcctg cagagattgc agaaccgatt aatggagaag
     5941 aaccgcaagt tcaagctatg caaaatgctt tgcggaaagc agaaattgtt aaaaataaag
     6001 tggataaaga tgcagtagtt atagctgcag atactttagt agccatagat aatcacatct
     6061 taggcaagcc cttaaaccaa aaagaagcaa gcagatattt gagattactt tcaggaaaca
     6121 ctcacagcgt ttataccggt atctgtattt tctataatga gatagcgaac ataaactatg
     6181 agcagacctt agtgcatttt gccgaattga ctgagttaga aattaaatcg tatatagaaa
     6241 caggagaacc tatggataaa gcaggagctt atggaattca gggttatgga gcacaattta
     6301 tcacagcagt tgatggatgt tattttaatg ttatgggttt tccagttcgt aaattctatg
     6361 aaatgctgaa tgccatatta gaaaaggaag agaaatgaac atattagcca acctctatct
     6421 ctggatagag ggtaaattta ctgaagaacc gaaatcgcta cttgttcata atacaagtat
     6481 ttcagagata ttgccttatc ctgttccaga acaatataaa aacatctcta tgaaagattt
     6541 aaatggttct tatgcctatc ctggctttat tgatacgcac acgcacagtt ttgaaggagg
     6601 attatattct ctgggagtgg atttgtctac tgctaaaaat atagaagaca tcctggctct
     6661 gctttcggca gcaaattcca aaaagacaaa ggacgagttc atttttgctt ggcaaatgga
     6721 tgagacatta ttaaaagaaa ggcggtttcc cactctaaca gaactaaata gtgctgttcc
     6781 cgacaaaccc ttaattttaa gaaggataga tggtcattcc tgtatgctca attcttatgc
     6841 aagaaatatg gttacaggtt tgaaaactaa agaagagatt ttgcgaggag gggaaaatga
     6901 ttatgctgtc catttttttc ataaacgctt aaatgatgaa gcaattttaa atgcttatcg
     6961 ggcagcagca aatattggtt tacaggcagg ttttacttct ctacatacta tgattggtga
     7021 tgcagaaaat agtattaatc actacgagct catcagagat aatttaaata aattccccct
     7081 acgatttatt ctatatcctc aatcttttaa tatcaaagca gctttggagg ctggagctaa
     7141 tagaatcgga ggttgtatat tagctgatgg ttcaatcggt tctgaaactg cagctatatc
     7201 agaaccatat ttaaatagta attcttgggg agttctttat caaagcgatg aattctggca
     7261 aaaatttatt acagaagcaa gtcaacaccg gctccaggta gctatacatt gtattggtga
     7321 tcgagcaatt cgccaaatta ataatgttta caaatcacta gctagcacag cagatttaag
     7381 acatcagctt atccactgtg aaataactcc cgataactta atcgaagaaa ttgctacttc
     7441 tggagctgtt cctgttatgc aaccaaattt tgattattta tggggaggtg aaaatggttt
     7501 ttacgctaaa aaaataggga taaagcgtag tagaataatg aaccgttttg ctactttatt
     7561 aaaatccggc attactatta ccggtggttc cgattggtat ataacatctt taaatgcagc
     7621 acattcactt agggcagcaa tcaatcatca caatccctta gagcgtttat ctactgcgca
     7681 agcaattgat atttatacta aaaacgctgc ctggttaaat agtgaagaag atacttatgg
     7741 gaaaatcaag attggatata aggcagattt atctatctat tcacacccaa tagaaaatga
     7801 aacaacagag ctttactatc ttatccgcga cggggaaata aaatatgcag ctatctgata
     7861 atgatttaat taaagttgat atcaaacagg ttattaagca ggaattactt tttcatcctg
     7921 aattgcaact gattgacctc tataaactcg tttttcaggc atatatggga ccgggacatc
     7981 taattaacaa tattgacgaa gcagcaaatt atattataac agaaatgctg ctacattctg
     8041 aaccatattt acctataaaa caggatatcg gtaacggaaa aggatttttt cgtgtaagtt
     8101 tagaccttct gatgccggaa aaagagctaa aaaaagaaca ggcaattgt
//
LOCUS       DAORHB010000049         7937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_8723_length_7937_cov_4.337351, whole genome shotgun sequence.
ACCESSION   DAORHB010000049 DAORHB010000000
VERSION     DAORHB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8723_length_7937_cov_4.337351"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..728
                     /gene="rplA"
                     /locus_tag="PKM80_02545"
     CDS             24..728
                     /gene="rplA"
                     /locus_tag="PKM80_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNV92816.1"
                     /translation="MKHSKRYNVAYSMFDRQKRLDLNEAVKLIKSLPASKFDETVEVH
                     FNLGVDPRKADQQIRNSLVLPHGTGKEMRVLVFAEGDKAEEAKQAGADYVGVDDLIEK
                     ISGGWFDFDVVIATPNLMGRIGKLGRVLGPRGLMPNPKVGTVTMDVGKAVQEAKGGKV
                     TYRVDKFGNLHIPAGRLSFDEDKLKENIKAIIAAVLRERPSTLKGIYIKSITLCTTMS
                     PGIKIQVASATLEARS"
     gene            742..1278
                     /gene="rplJ"
                     /locus_tag="PKM80_02550"
     CDS             742..1278
                     /gene="rplJ"
                     /locus_tag="PKM80_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNV92817.1"
                     /translation="MVQSIKYDIVTDLTARLSGAKAIVLVDYKGINIEQVNQLRNRFR
                     ESQVDYFVQKNTLIKIALNGLGITELDPYLNGPTAVAVCKMDEVSPARELTKFLKEVM
                     ENKNYPSFKVGYIAGHIFDAVELTALAKLPSREELLAKVLSCMNAPLSNFVSINQGII
                     RKFVYALDAIVKKQAEAS"
     gene            1312..1692
                     /gene="rplL"
                     /locus_tag="PKM80_02555"
     CDS             1312..1692
                     /gene="rplL"
                     /locus_tag="PKM80_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNV92818.1"
                     /translation="MSDKKQQVIDLIKEMTVLELSELVKEMEEVFGVSAAAPVAVVAA
                     TQGAAEGGVKEEQTEFDVILTSAGEKKINVIKVVREITKLGLKEAKDLVDSAPKMVSE
                     KVSKEEAESIRKKLEEAGATVEVK"
     gene            1911..5690
                     /gene="rpoB"
                     /locus_tag="PKM80_02560"
     CDS             1911..5690
                     /gene="rpoB"
                     /locus_tag="PKM80_02560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNV92819.1"
                     /translation="MRKVKSYSRISGRFAELGIPEVEIPNLLAMQVDSFNEFLQKDIH
                     PQRREKKGLQAVFENAFPLEDAKGNFLLEFVEYSVLQEKYTIEECRDRNLTYQAPLKA
                     KMRLSVFENNEGVREHKDTLEQEVFLGEIPLITEQGTFVINGAERVIISQLQRSPGVF
                     FSEEKHPSGKTLYSAKVIPYSGSWLDFDIDIHDVMYVHIDKRRKLPVTTLFRAIGIST
                     NEDLRKTFYKTEQLNLKSAKGRHLYADIYETDNPEPIALANEQITDSLIKFLTERKIT
                     KVEVIDYNYEIARKVLENTLAKDPTTNQEEALKKIYALIRPGEDAPIEVAKQLVDRMF
                     FNEKRYNLGEVGRYKMNTRLGINVDPKVMTLTVEDFVYIFKTLINIYHDEDEVDDIDH
                     LSNRRVRTVGELLQEQYTSGMAMISRIIQERMAISNIDEVTVHDLVNSNALISVVQSF
                     FLTGQLSQFMEQTNPLAALRHKRALSALGPGGLTRERAGFEVRDVHSSHYGRVCPIET
                     PEGPNIGLIVSPALYSRINYFGFIETPYRKVVDGCVTDEFEYLDAAQEEKYIIAQSDI
                     HLDDNRRITDDVIFARDRGDFVQVNPKNVQYMDIAPQQMVSVSAALIPFLEHDDANRA
                     LMGSNMQRQAVPLINPQAPLVGTGMEKIVSMDTSAIAVAPYDGIVTQVTSAFIDLKPI
                     SEKDEAFYLSIGNRIYLKKFVRTNQDTCINQRPIVRIGDNVKKGQPISDGACVEDNRL
                     ALGTNMMVAFMPWYGYNYEDAIILSEKVARDDSLTSIYIEELEVLVRNVKNGREELAY
                     DIPNVPAQALKNLDKTGIIRVGSVIRAGDVIVGKVTPKSVEIDPSPEENLMRALFGDR
                     AGDFTNSSLKAKPGMEGVVIDVKVFSRLEEGSDLEEENEDRIEKLKEELALRRRKIEE
                     FKEEKLSSVLVGQIAKNIWDEKTNAPFIAPGKKITKEDIKRINFKKLDLDVELVEDSE
                     VNNYIYYQIVLKIKQSLEQSDNIYKKSRERIKHGDELQYGVRKMVKVYVAKKRKIEVG
                     DKMAGRHGNKGVISIVAPVEDMPFMEDGTPVDIVLNPLGVPSRMNIGQIMETHLGWAA
                     KTLGFEVETPIFDGASNEDIRSELRKAGLPEDGKTVLYDGKSGEPFKERVTVGVIYMM
                     KLNHLVADKMHARSTGPYSLITQQPLGGKAQHGGQRLGEMEVWALEAYGASHVLEEML
                     TIKSDDVDGRNNAFKAITKGENPPPPGVPESFNVLISELKSLGFDIEFIKEEERR"
     gene            5705..>7937
                     /gene="rpoC"
                     /locus_tag="PKM80_02565"
     CDS             5705..>7937
                     /gene="rpoC"
                     /locus_tag="PKM80_02565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNV92820.1"
                     /translation="MLKDKYEIKPNEYDAVRIKIASPDTIRHEWSHGEVTKPDTLNYR
                     TLKPEKDGLFCERIFGPERDYECACGKYKKKRFQGTVCDRCNVLVTTSRVRRTRMGHI
                     ELAVPIAHIWFVKSAPSKIGTLLDMTVKDLERILYYESFIVIDPGDSPYEKMELLEVD
                     EYYEIKDKLGDNFLALMGGEAIQELLKRINLKNEALDLRTRLKFEESALRKQKLINKL
                     KIVDAFIKSGNRPEDMILEVLPVLPPTLRPLVPLEGGRFATADFNDLYRRVITRNNRL
                     KQLLEIRAPEVILRNEKRMLQEAVDALIDNGRKARPVRGRGNRPLKSLTDQLKGKQGR
                     FRQNLLGKRVDYSGRSVITVGPELKLYQCGLPKEMAVELFKPYLIEKLQKMGEVDKVK
                     NAKKLIEKKQPEIWSILEEVIKDYPVMLNRAPTLHRLGIQAFMPVLTDNKAIQLHPMV
                     CVPFNADFDGDQMGVYIPLSLEAQIEARVLMLSTRNLLLPANGRLAMAANQDIVLGCY
                     YLTMEETPAPKDIEKLRHFYGPDEVIAAYEEEEQLSIAKGDSVFERNLDLHTWIRLKL
                     ESEYIITTVGRVIFNQIMPPEVGFQNYVFDKGKLNDLAMLCFDAVGQWRTAQILDKVK
                     ELGFSYATRAGVTFSFEDIIVPERKNDIVAQSEGEVKKINDLHQKGGITENERFGRVV
                     DQWKKTTVRVTDEMMEELSRTRNGLNSIYMMYKSGARGSKDQIKQLGGMRGLMDKPTK
                     IGAT"
BASE COUNT         2626 a         1324 c         1752 g         2235 t
ORIGIN      
        1 gatagtattc ataaggagag tcaatgaaac atagtaaaag gtataatgtt gcctattcga
       61 tgttcgatcg tcaaaaaaga ttagacctta acgaagctgt taaacttata aagagtttac
      121 ctgcttccaa gtttgacgaa acagttgagg tccattttaa tttgggtgtc gatcctcgaa
      181 aggcagatca gcaaattaga aactctcttg tcttgcctca tggaactggt aaagagatgc
      241 gagttttagt ttttgctgaa ggtgacaagg cagaagaagc caaacaagct ggtgcagatt
      301 atgttggtgt ggatgatctc atcgaaaaaa tatcgggcgg atggttcgat tttgatgtgg
      361 tgattgccac tcccaattta atgggacgca tcggaaaact gggacgcgtt ttaggcccca
      421 gaggattaat gcccaaccct aaagtaggaa ctgttacaat ggatgttggt aaagcagtgc
      481 aggaagctaa aggtggaaaa gttacttacc gtgttgataa gtttggtaat ttacatattc
      541 ctgcaggtag acttagcttt gatgaagata aactaaaaga aaacatcaaa gctattattg
      601 ctgctgtgct tagagagaga ccttctactt taaagggtat ttatattaag agcataaccc
      661 tctgcacaac tatgagtcct ggaatcaaaa ttcaggtcgc aagcgcaacc ttggaagcaa
      721 gaagctaagg ggagtatgaa gatggttcaa agtattaagt atgacatagt gacggacttg
      781 acagcaagac taagtggcgc aaaagcgatc gtattggtag attacaaggg aataaacatc
      841 gagcaagtga atcaattgcg caaccgcttc agagaaagtc aagtggacta ctttgtccaa
      901 aaaaataccc taattaaaat tgctttaaat ggtttgggta ttacagaatt agatccgtat
      961 ctcaacggac caaccgctgt ggcagtatgt aaaatggatg aagtatcccc tgcccgcgag
     1021 cttactaaat tcttaaaaga ggtgatggag aataagaatt atccttcgtt caaagtcggt
     1081 tatattgcag ggcatatatt tgatgctgtt gaattgactg ctttggcaaa acttccatcc
     1141 cgcgaagaac tacttgccaa agtgttaagc tgtatgaatg ctccgcttag caactttgta
     1201 agtattaatc agggtatcat ccgcaagttt gtttatgctc ttgatgccat agtaaaaaaa
     1261 caagctgaag ccagctaaaa aaaaattatt catatatccg atggaggaac aatgtccgat
     1321 aaaaagcaac aagtaattga tctaattaag gagatgacag ttcttgagct ctctgaattg
     1381 gtaaaagaga tggaagaggt ttttggtgtt tccgctgctg ctcctgtagc tgttgttgcc
     1441 gcaacacaag gtgctgctga aggtggcgta aaagaagaac agacagaatt cgatgttata
     1501 ttaaccagtg ccggcgagaa aaaaatcaat gtgattaaag ttgtaagaga gataaccaag
     1561 cttggtctca aggaagcaaa agaccttgtt gatagtgcac ccaagatggt ttctgagaaa
     1621 gttagcaagg aagaagcaga atcaattagg aaaaaactcg aagaagccgg tgcaactgtc
     1681 gaagtcaaat aatcgaacca taatcaggat aaaggtccaa aaaaagtcct gcgggacttt
     1741 ttttggccta atttcccaat ccaattatcc ataaatgatg gtttttttaa ctataagcca
     1801 gcaaaccaat tttccataca tcaatggcgc aacaacaagc gtgagcaaaa ttgagattaa
     1861 caaaaagagc ccaaaatgtt aggaaataaa cacctaaaag gagagagctt ttgagaaagg
     1921 ttaaaagtta ttcacgaata tccggaagat ttgctgaact cgggattccg gaggtagaaa
     1981 tacccaatct tctggctatg caggtggatt ccttcaatga atttctgcaa aaggatattc
     2041 acccgcaacg cagagagaaa aaaggcctgc aagccgtatt cgaaaatgca tttcccttgg
     2101 aagatgccaa aggtaatttt ctgttagaat ttgttgaata tagcgttttg caggaaaagt
     2161 ataccattga ggaatgtcgg gaccgaaacc tcacttatca ggcgcctctt aaagccaaaa
     2221 tgcgtctaag tgtgtttgaa aataatgaag gtgttcggga gcataaagac actttagaac
     2281 aagaagtttt cttaggagaa atccctctga ttacggaaca gggaactttt gttatcaatg
     2341 gagcagaacg tgtcattatt tcacaattac aacgttcgcc cggagtgttc ttttctgaag
     2401 agaaacatcc cagtggaaaa acactttatt ctgcaaaagt gattccctac agcggttcgt
     2461 ggctggattt tgatatagat attcatgatg tgatgtatgt gcatatcgat aaacgtcgta
     2521 agcttccagt aactacactt tttcgtgcta tcgggatatc cactaatgag gatttgcgaa
     2581 agacctttta caaaaccgag cagctgaatt tgaaaagtgc taaaggtcgc catctctatg
     2641 cagatatata tgaaaccgat aatcccgaac ctattgcttt agcaaacgag cagatcactg
     2701 attccttgat aaaattttta actgaaagaa aaatcactaa ggtggaagtg attgattata
     2761 attatgaaat tgccagaaag gttttggaaa atactctggc aaaagaccca accaccaacc
     2821 aagaagaagc tctgaaaaaa atctatgcct taatacgccc tggtgaagat gcgcctatag
     2881 aagttgctaa acagcttgta gatagaatgt ttttcaatga aaagcgttac aacttgggag
     2941 aggtcggtcg ttacaaaatg aatacccgct taggtataaa tgtagatccc aaagtgatga
     3001 ctcttacggt tgaggatttt gtatatatct ttaaaacttt aataaacatc tatcacgatg
     3061 aagatgaagt ggatgatatc gatcatttat caaatagaag agtacgaact gttggtgaat
     3121 tgctgcagga acaatatact tcaggaatgg ctatgatatc cagaataatt caagaacgga
     3181 tggccatttc caatattgat gaagttactg tgcacgatct tgtaaatagt aacgctctta
     3241 tttcagtagt tcagtcattc tttttaacag gtcaattatc ccaatttatg gagcagacta
     3301 atccacttgc agctttaaga cataagcgtg ctctttctgc tctaggtcct ggaggtttaa
     3361 ctcgtgagcg agctggtttt gaagttcgag atgttcattc atctcactat ggacgtgtgt
     3421 gtccaattga aacaccggaa ggaccaaata ttggattgat agtttctcct gctttatatt
     3481 ccaggataaa ctattttggc ttcatagaaa ctccttaccg gaaggttgtg gatggatgtg
     3541 taactgatga atttgaatat cttgatgctg cccaagaaga gaaatatatt atagcccaaa
     3601 gcgatattca tttggatgat aaccgtagaa ttaccgatga tgttattttt gccagagatc
     3661 gaggtgattt tgttcaggtt aaccctaaaa atgttcagta tatggacata gcacctcaac
     3721 agatggtttc agtatcggca gctttgattc catttttaga gcatgatgat gcaaaccgtg
     3781 cattgatggg ttcaaatatg caacgacagg cagttcctct gataaatcct caagcacctc
     3841 ttgttgggac tggtatggaa aaaatcgtct ctatggatac ctcagcaatt gctgtagctc
     3901 cctatgatgg aattgttact caagtaactt ctgcttttat tgatctaaaa ccaattagtg
     3961 agaaagatga agcgttctat ttatccatcg gaaaccggat ttatctgaaa aagtttgtcc
     4021 gtactaatca agatacttgt atcaaccagc gtcctatagt tcgcataggt gataatgtta
     4081 aaaaggggca gcctatatct gatggagctt gtgtagaaga taaccgtttg gctttaggga
     4141 ccaatatgat ggttgccttt atgccttggt atggttataa ttatgaagat gctattattt
     4201 taagtgaaaa agtagcaaga gatgattctc tcacttccat ttatattgaa gaattggaag
     4261 tcttggtaag gaatgtgaaa aacgggcgtg aggaattggc ctacgatatt cctaatgttc
     4321 ctgctcaggc attgaaaaat cttgataaaa ctggcattat cagagtaggt agtgtgatta
     4381 gggctggaga tgtaattgta ggtaaggtta ctcctaaaag tgtagaaatt gatccttctc
     4441 cggaagaaaa tttgatgcgt gctttgtttg gagaccgagc aggtgatttt accaatagct
     4501 ctttaaaagc caaaccggga atggaaggag tggttatcga tgttaaggtg ttttcccgtt
     4561 tggaagaagg cagcgatttg gaagaagaga atgaagatag aattgaaaag ctgaaagaag
     4621 aattagcttt gcggcgtagg aaaattgagg aatttaagga agaaaaactt tcatcggtgc
     4681 ttgtgggaca gattgctaaa aatatctggg atgagaaaac caatgcaccc tttattgctc
     4741 ctggtaagaa aattactaaa gaagatatca aacgcattaa cttcaaaaaa ctcgatttgg
     4801 atgttgaatt agttgaagat agtgaagtga ataattatat ctattaccag atagtcctga
     4861 aaatcaaaca atcactcgaa caaagcgaca acatttataa aaaatcacgg gaacgaatca
     4921 aacatggtga tgaacttcaa tatggcgtcc gtaaaatggt taaggtatat gtagccaaaa
     4981 aacgcaaaat tgaagtagga gataagatgg ctggacgcca tggtaataaa ggtgtaatat
     5041 ctattgttgc ccctgtcgaa gatatgcctt ttatggaaga cggcacgcct gtagatatag
     5101 tactaaatcc tttaggtgtt ccatctcgta tgaatatcgg tcagattatg gaaactcatt
     5161 taggatgggc agcaaagacc cttggcttcg aagttgaaac ccctattttt gatggcgcat
     5221 ccaatgaaga tattcgttcc gaattgcgaa aagcaggtct accagaagat ggcaagacag
     5281 ttctttatga tggtaaatct ggtgagcctt ttaaagaaag ggtaacggtg ggagttatct
     5341 atatgatgaa actgaatcac ctagtagccg ataaaatgca tgcacgttcc acgggacctt
     5401 attcacttat aactcaacaa cccttaggtg gaaaagctca gcatggtggt cagcgattag
     5461 gagaaatgga agtttgggct ttggaagcct atggagcttc tcatgttctg gaagaaatgc
     5521 taacaatcaa aagtgatgat gtggatggtc gtaacaatgc attcaaagct attactaaag
     5581 gcgaaaatcc acctcctccc ggagtgcctg aatcctttaa tgttctcatc agtgaactga
     5641 agtcactggg ctttgatatt gaatttatca aagaagaaga gagaaggtaa cggagggaaa
     5701 taggatgtta aaagataaat atgaaattaa gccaaacgaa tatgatgcag ttcgcataaa
     5761 gattgcttct cctgatacta ttagacatga atggtcccac ggagaagtaa caaaacccga
     5821 tactctaaat tatcgaactc ttaaaccaga aaaagatggt ttgttttgtg agagaatatt
     5881 tggaccggaa cgggattatg aatgcgcttg cggaaaatat aaaaagaaac gctttcaggg
     5941 cactgtttgt gatcgttgta atgttttagt aacaacttct cgcgtacgaa gaactcgtat
     6001 gggacatatt gaattagcag tgcctattgc tcatatctgg tttgtgaaaa gtgctcctag
     6061 caaaattgga actttacttg atatgacagt taaagacctc gaaaggattc tttattacga
     6121 gtccttcatt gttattgatc cgggcgatag tccttatgaa aaaatggaac tgctggaagt
     6181 tgatgaatat tacgagatta aagacaaatt gggagataac ttcctggccc ttatgggtgg
     6241 agaagcaatc caagaattgc tcaagcggat taatttaaaa aatgaagcgc ttgaccttag
     6301 gactcgtctt aaatttgaag aatcagcctt gcgtaaacaa aagctgatta ataagcttaa
     6361 aatagtagat gcctttatca aaagtggtaa tcgtcccgaa gatatgattt tggaagtttt
     6421 acctgtgctt ccgccaactt taagaccatt agttcctttg gagggtggcc gctttgcaac
     6481 tgctgatttt aatgacttat atagaagagt tattaccaga aataaccgtt tgaaacagct
     6541 tttagaaatc cgtgctccag aagttatttt acgcaatgaa aaacgaatgt tacaagaagc
     6601 agttgatgct ttaattgata atggtagaaa agcacgtcca gtacggggca gaggaaatcg
     6661 tcccttaaaa tcattaacag atcagcttaa gggtaaacaa gggcgttttc gtcaaaatct
     6721 tttgggtaag cgagtagatt attctggccg tagcgttatt acggttggtc ccgaactaaa
     6781 actttatcaa tgtgggcttc ccaaagaaat ggcagttgag ctctttaagc cctatcttat
     6841 tgaaaaacta caaaaaatgg gggaagtaga taaagtaaaa aatgccaaaa aactaataga
     6901 gaaaaaacaa ccagaaatat ggtctattct ggaagaagta ataaaagatt atcctgttat
     6961 gctaaaccgt gctccaactc ttcaccgttt aggtattcaa gcatttatgc ccgtattaac
     7021 agataacaaa gctatccaat tacatccaat ggtttgtgtt ccttttaatg ctgattttga
     7081 tggtgaccaa atgggggttt atatccctct ttctctggaa gctcaaattg aagccagagt
     7141 attgatgctc tctactcgca atctgttatt acctgcaaac ggacgtttag ctatggctgc
     7201 aaaccaagat atcgttttgg gatgctatta tctaacaatg gaagaaactc ctgctcccaa
     7261 agatattgaa aaattgcgtc atttttatgg tcctgatgaa gttattgcgg cttacgaaga
     7321 agaagaacaa ttaagcatag ctaaaggtga ttctgtcttt gaacggaatt tagacctaca
     7381 tacttggatt cgcttaaaac tggaatccga atatattatc acaaccgtag gcagggttat
     7441 tttcaatcag attatgcccc cagaagtagg ttttcagaac tatgttttcg acaaaggaaa
     7501 actgaacgat ctggctatgt tatgttttga tgctgttgga caatggagaa ctgcacaaat
     7561 tttggacaaa gttaaagaac ttggttttag ctatgctaca agagcaggag taacattcag
     7621 ttttgaagat ataattgttc ctgaacggaa aaatgatata gttgctcaat ctgaagggga
     7681 agtaaaaaaa ataaacgacc ttcatcaaaa aggtggaatt accgaaaacg aacgttttgg
     7741 acgagttgtt gaccaatgga aaaaaactac agtgcgggtt acagatgaaa tgatggaaga
     7801 actctccaga acaagaaatg gcttgaactc tatttatatg atgtataaat ctggagcgcg
     7861 cggcagtaaa gaccagatta aacaattggg tggaatgcgt ggtttgatgg ataaacctac
     7921 aaaaataggt gccacgg
//
LOCUS       DAORHB010000050         7933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_8734_length_7933_cov_4.361132, whole genome shotgun sequence.
ACCESSION   DAORHB010000050 DAORHB010000000
VERSION     DAORHB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8734_length_7933_cov_4.361132"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PKM80_02570"
     CDS             <1..1002
                     /locus_tag="PKM80_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNV92821.1"
                     /translation="MGDINNNPFLKDGDVVYVPAIKNFVSIGGGINLPGELEFVEGDK
                     ISTIIELSLGFTFDADISKLQLYRYKENRIDYDVLNYDLKANPSLLNLPLQADDRILI
                     SCDSEIRTRQRIKIYGQVKNPGEYVIDTNTTLYEVLQQAGGLTKRGNIKTMVYYNENI
                     NVDPDPYFEMLMQRSLNEMTPLEYSYLRTNLQQLKGKYSIDPVKMMDSEGKDSNPTLF
                     DGDIIYIPEKINMVWVSGQVKNPGMIPWMEGKDWSYYIQAAGGYTNNRKTGKDRIIRG
                     TSGNWVKPNKKVAIQPGDTIFIPAQTDRSLWTDVKDIVTLTSSVVTIILGIHALTAN"
     gene            1021..2127
                     /locus_tag="PKM80_02575"
     CDS             1021..2127
                     /locus_tag="PKM80_02575"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV92822.1"
                     /translation="MNVPMLDLKAQYVPVMGRIKEALEGVFTEHQYIMGPPVKEFENK
                     MADYLGIKNAIGCASGTDALVLAIKALEIGEGDEIITTPFTFFATASSISRNNATPVF
                     VDIDPLTFNLDPDKIEAAITEKTKAILPVHLFGQCADMDSIMAIAQKYNLKVIEDNAQ
                     GIGCTWNGKMSCAFGDIGTLSFFPSKNLGAMGDAGMCMTNDDDLAFKLRQLRVHGENP
                     KYYHKWIGLNSRLDTLQAAVLGVKLDFLETWSKGRRANADFYNSHLKNIPGIRIPHID
                     SRAVSIYNQYTLVCEKRDELMAYLKSKGIGCAVYYPLPLHLQECFSSLGYKKGDMPVA
                     EELSLKVLSIPIYPELTDEQKQYVCETITNFYFG"
     gene            2140..2925
                     /locus_tag="PKM80_02580"
     CDS             2140..2925
                     /locus_tag="PKM80_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNV92823.1"
                     /translation="MHYIVCVKQVPNTTEIKIDPKTNTLVREGVESILNPFDAYAVEE
                     AVRLKEKYGGTVTAISMGPNQCETTLRETVSLGVDNIILLSDRRFAGADTLATSLTLA
                     AAIRKIGDYTLILTGQQAIDGDTGQVGPGIAAHLKIPQTCYVRKIESFSENSAVVERL
                     LEDGYDRISMQLPAVISVVKEINVPRLPSLRGKKNAKTAVLTVWNCDDLGLNEKETGL
                     NGSPTQVMSIFSPHHEKQVEKFEGNSDEAVELIIEHLSKLTHR"
     gene            2922..3752
                     /gene="lgt"
                     /locus_tag="PKM80_02585"
     CDS             2922..3752
                     /gene="lgt"
                     /locus_tag="PKM80_02585"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNV92824.1"
                     /translation="MITYPNIDPNIVSFSIGNLNLHIRWYGLFYVLSFILAFILYKPF
                     LKKRNIKLTKEQYESIIFYMMLGVILGGRLGYILFYNLRYYLSHPIMIFAVWEGGMSF
                     HGGALGVIIAGLIFCHKHKLNFYAMADSAIPIVAIGLGLGRLGNFINAELYGKPTNLP
                     WGMIFPGSDGAVRHPSQLYELILEGIVMFVITYFLLKKLKKEGVVFWSFIGLYGIFRF
                     LIEFVREPDDLDFYTRSGYIFGFMSIGQFLSLLMMIIAIWGFYSIYKTPKKQEQRKTN
                     "
     gene            3783..4514
                     /locus_tag="PKM80_02590"
     CDS             3783..4514
                     /locus_tag="PKM80_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92825.1"
                     /translation="MKKTVLVLLTITAIIITACAGPKGISENEKLRLELKKWESFDSQ
                     GVVELSYKGLAIRKMFIASKNGNELRFDIIDGGLMGSKAEPLMSVYVADYVSIRSPFI
                     PMLESLDISQAIPMESLTLSTNVDSLVTHYAQKIIKDKKIELNGVVISFSKNYLLDTV
                     LDSKSNTKIKALYTGNSKLSELYITSNDDISIRLSFDKIEYIQPHIVPLPKAEPSSLE
                     NIIQDLNKISIDSLLRDYLQYRKEK"
     gene            4517..5812
                     /gene="purB"
                     /locus_tag="PKM80_02595"
     CDS             4517..5812
                     /gene="purB"
                     /locus_tag="PKM80_02595"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNV92826.1"
                     /translation="MITRYSKPEMERIWTQENRYECWLEVELAAAKAMYEKGMIPAED
                     WETINSKADFDSNRIDELEQVTKHDVIAFLTNVAENIGPASRWIHFGMTSSDVLDTAT
                     GMQLKSSGELILTELSRLAEILKLKARQYRNTICIGRSHGIHAEPTSFGLKFALWYEE
                     IQRNIIRLNEAIEEVSVGKFSGAVGNFAHLSPEIEDMACKFLDLKPAKVSTQIIQRDR
                     YAYFLSVLALIASAIEKMALEIRHLQRTEVHEAEENFSKGQKGSSAMPHKRNPIISEQ
                     LCGLSRVLRSNALSAMENNALWHERDISHSSVERIILPDSCILIHYMLNKTCSLIENL
                     VVYPENMKKNLELTKGLVFSQALLLHLVKSGLTREKAYALVQQEAMKSFETNTNFLDN
                     VLANKEITTALAESEIRDIFSYERYLRNVDYIFRRCGIL"
     gene            5828..6277
                     /locus_tag="PKM80_02600"
     CDS             5828..6277
                     /locus_tag="PKM80_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNV92827.1"
                     /translation="MKHYFLILIASIICVPILLSGKEETDRIIGYWLTGDKATKIEIY
                     QNEDGTFAGKIIWLQEPNDAKGNPKKDINNPDVKLQQQPLIDLIVLSDFVRENRNKYK
                     NGKYYEPTTGKTYSAKIELINNNTITIRYFIGVPTLGRTDTWIRSSN"
     gene            complement(6575..6697)
                     /locus_tag="PKM80_02605"
     CDS             complement(6575..6697)
                     /locus_tag="PKM80_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92828.1"
                     /translation="MDKKVTQRSTYKAFKMKIVEERHPAVPLLTAKLCSYRYML"
     gene            complement(7249..7872)
                     /locus_tag="PKM80_02610"
     CDS             complement(7249..7872)
                     /locus_tag="PKM80_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV92829.1"
                     /translation="MSNRLAVAYQKANRLDDAISKYETIIKNNPQNTMAYLNAVNLYR
                     LKASESTDPAFVASINKKAIDTLSALIKQHPDNALAYMNMAAIYLGQNKYQEAETYAL
                     QALKKDPTLYLPYVYLATISQSKGTNDYNHFIDLEKQAAKAVGTKAKTLKNQRDNAKS
                     SAVSNFRKALDYLNNAKVRATSEEAITDINNRIARVNQLITQATSTY"
BASE COUNT         2525 a         1310 c         1536 g         2562 t
ORIGIN      
        1 atgggagata taaataataa cccttttcta aaggatggag atgtggttta tgttccagcc
       61 atcaaaaatt ttgtttccat cggcggaggt attaatcttc caggtgagct ggaatttgta
      121 gaaggcgata aaattagcac aataatcgaa ctttccttag gtttcacttt tgatgctgat
      181 atttctaaac ttcagctcta tcgttataaa gaaaaccgta ttgactatga tgttctaaac
      241 tatgatctta aagccaaccc ttctttatta aacttaccct tacaagctga tgatcgtatt
      301 ctaatatctt gcgattcaga aattaggacc cgccaaagaa ttaagatata cggtcaggtt
      361 aaaaatcccg gtgaatatgt tattgatacc aatacaactc tttacgaagt tctccagcaa
      421 gcaggtggtt taacaaagcg gggcaatata aaaacaatgg tctattataa tgaaaatatt
      481 aatgttgatc ctgatcctta ttttgaaatg ctgatgcaaa gaagcttgaa tgaaatgacc
      541 cctttagaat attcatattt gagaaccaat cttcagcaac tgaaaggaaa atacagtata
      601 gaccccgtta aaatgatgga cagtgaagga aaggattcaa atcctactct tttcgacgga
      661 gatattattt acatcccaga aaaaatcaat atggtttggg ttagtggaca agttaaaaat
      721 ccaggtatga taccttggat ggaaggtaag gattggagtt attatattca agctgctgga
      781 ggttacacga ataatcgcaa aacaggcaaa gatcgtatca ttcgtggtac cagcggaaat
      841 tgggttaaac ctaataaaaa agttgctata caaccaggag atactatttt tattcctgct
      901 caaactgatc gttctttatg gactgatgta aaggacattg taacgctaac ttcatcagtt
      961 gtgacaatta ttcttggaat tcatgcactt acagcaaatt aagaaggaga tgaaacaata
     1021 atgaatgttc ctatgttgga tcttaaagca caatatgtac ctgtaatggg aagaattaaa
     1081 gaagcactgg aaggtgtctt tacagagcat caatatataa tggggccacc tgttaaggaa
     1141 tttgagaata aaatggctga ttacttaggg attaagaatg ccattggttg tgcttctgga
     1201 acagatgccc ttgttttagc tattaaagca ttggaaattg gtgaaggtga tgaaattatt
     1261 actactcctt tcactttttt tgcaactgct tcttccatat cgcgtaataa tgcaaccccg
     1321 gtttttgtag atatcgatcc gctaactttt aatcttgatc ccgataaaat agaagcggct
     1381 ataaccgaaa aaacaaaagc cattctacca gttcatttat ttggtcaatg tgccgatatg
     1441 gattctatta tggctattgc ccaaaaatat aatttaaaag ttattgaaga taacgctcag
     1501 ggtattggat gtacttggaa cggaaaaatg agttgtgctt ttggcgatat tggaactctt
     1561 tctttcttcc cttctaaaaa tcttggtgcg atgggtgatg ccggaatgtg tatgaccaat
     1621 gatgatgatt tagcttttaa attgcgtcaa ttaagggttc atggagaaaa tcctaagtat
     1681 tatcataaat ggattggttt aaacagtcgt ttagatacct tgcaggcagc tgttttgggt
     1741 gtaaaattgg attttcttga aacctggagc aagggtcgcc gagctaatgc agatttttac
     1801 aattcacacc tcaagaatat tcccggaata aggattcctc atatagattc tcgagcggtg
     1861 agtatatata atcaatatac cctcgtctgt gaaaaaagag atgagctgat ggcctatttg
     1921 aaatccaaag gcatagggtg tgctgtctat tatcctcttc ctttgcattt acaggagtgt
     1981 tttagttctc ttggttataa aaaaggagat atgcctgtgg ctgaagagct ttctcttaaa
     2041 gttctttcca tccctattta tccggagctc acagatgaac aaaagcaata tgtttgtgaa
     2101 acaataacta acttttattt tggttaaagg aaaataaaaa tgcattatat tgtgtgtgta
     2161 aaacaagttc ccaatactac agaaattaag attgacccca agaccaatac tttggtcaga
     2221 gaaggtgtag aaagtatttt aaatcctttc gacgcatatg ccgttgaaga agcagttcgt
     2281 ttaaaagaga aatacggcgg aacagttaca gccatcagta tgggtcctaa ccaatgtgaa
     2341 actacactgc gggaaactgt ttctttagga gtggataata ttattctttt gtcagatcgt
     2401 cgttttgcag gagctgatac acttgctacc agtttaactt tggctgctgc tataagaaaa
     2461 attggagatt atactcttat tttaacaggg caacaagcta ttgatggaga tactggacaa
     2521 gtaggtcctg gaattgcagc tcatttaaaa attcctcaaa cttgctatgt tcgcaaaatt
     2581 gaatcgtttt ctgaaaattc agcagttgta gaacgcttac tggaagatgg ttacgatcgt
     2641 atttcgatgc agcttccagc agtgatttct gttgttaagg aaatcaatgt cccccgcttg
     2701 ccttctttgc gtggaaagaa aaatgccaaa actgctgttc ttactgtttg gaattgtgat
     2761 gatcttggat tgaacgaaaa agaaactggt ttaaatggtt ctccaacaca ggttatgagt
     2821 attttttctc cccatcatga aaagcaggtg gaaaagtttg aaggtaacag tgatgaagct
     2881 gttgaattaa taatagaaca tctttcaaaa ctaacccaca gatgattaca tatccaaata
     2941 tagaccccaa tattgttagt tttagcatag gaaatctcaa tcttcatatt cgctggtatg
     3001 gtctctttta tgttctaagc tttatattgg ctttcatttt atataagcca tttttgaaaa
     3061 agcggaatat taaactaact aaagagcaat atgaatccat cattttttat atgatgttag
     3121 gagttatttt aggtgggcga cttggttata tactttttta taacctgcgc tattatcttt
     3181 ctcatcctat aatgattttt gctgtttggg aaggagggat gagttttcat ggaggagctt
     3241 taggagttat tatagctggg cttatctttt gccataagca taagctgaat ttctatgcaa
     3301 tggcagattc agcaattcct attgtggcta ttggtttagg attagggcgt ttgggtaatt
     3361 ttattaacgc agaattgtat gggaagccca cgaatttacc ttggggaatg atatttccag
     3421 gttcagacgg tgctgtaaga catccttctc agctctatga acttattttg gaaggcattg
     3481 ttatgtttgt gatcacttat tttcttttaa aaaagctaaa gaaagaaggg gtagtatttt
     3541 ggagctttat aggtttatat ggaatctttc gatttttaat agaatttgtg cgcgaacctg
     3601 atgacttaga tttttatact cgttctggct atatttttgg gtttatgtct attggccaat
     3661 ttctaagctt attaatgatg ataattgcca tctggggttt ttatagtatt tataaaacac
     3721 ctaaaaaaca ggaacaaagg aaaactaatt aactctttta tattaggaaa acgaggttgg
     3781 taatgaagaa aacagttctt gttctcttaa ccattacagc tattattatt actgcctgtg
     3841 ctggacccaa gggcattagt gaaaatgaaa aattacgcct ggaattgaaa aaatgggaga
     3901 gttttgatag tcagggagta gttgagctat cctataaagg acttgctatc cggaaaatgt
     3961 tcatcgcttc caaaaatggc aatgagctcc gttttgatat tatcgatgga ggccttatgg
     4021 gttctaaagc agaacccttg atgagtgtat atgtggcgga ttatgtatct attagatccc
     4081 cctttattcc aatgttggaa tctttggata tctcgcaagc aattcctatg gagagtttaa
     4141 ccctttctac taatgtagat tctctcgtca cccactatgc acaaaaaatt attaaggata
     4201 agaaaattga attaaacgga gtggtaattt ctttcagcaa gaactatctt ttggatactg
     4261 tgttagatag taaaagcaat acaaaaataa aagcacttta cactggtaat agtaagcttt
     4321 ctgaattata tattactagc aatgatgata tcagcattag attaagcttt gataaaatag
     4381 aatacatcca acctcatatt gttcctttac ccaaagctga accttcctct ctggagaata
     4441 taatacagga tttaaataag ataagcattg attcgttgct gagggattac ctgcaatata
     4501 gaaaggagaa ataaaaatga taacccgcta cagtaaaccc gaaatggaac gtatttggac
     4561 tcaagaaaat cgttatgaat gttggctgga agtagaactt gctgccgcaa aagcaatgta
     4621 tgaaaagggt atgattcctg cggaggattg ggaaactatc aattctaagg cagattttga
     4681 ttctaaccga attgatgaac tggagcaggt tactaagcat gatgttatcg cttttttaac
     4741 caatgtagcc gaaaatatag gtcccgcatc gcggtggatt cattttggaa tgacctcttc
     4801 agatgttcta gatacagcca cgggaatgca gttaaaaagc tctggagaat taattttaac
     4861 ggaactcagt cgtttggcag agattttaaa gttgaaagcc agacaatata gaaatacaat
     4921 ttgtatcgga cgttctcatg gaattcatgc agagcccact tctttcggac tcaagtttgc
     4981 tctttggtac gaagaaattc agcggaatat aatccgttta aatgaagcta tagaagaagt
     5041 aagcgtaggt aaattttcag gcgcagtagg aaattttgct catcttagcc ctgaaattga
     5101 agatatggct tgcaagtttt tggatctcaa acctgccaaa gtatcaactc agataattca
     5161 aagagataga tatgcatatt tcctttctgt tttagctctt attgcttctg caatagagaa
     5221 aatggctctg gaaatcagac atctacaaag gaccgaagtt catgaagcag aagaaaattt
     5281 ctccaaaggc caaaagggct cttcagcaat gcctcataaa cgcaatccta ttattagtga
     5341 acaactttgt ggtctatcaa gagttttgcg ttccaatgct ttaagcgcaa tggaaaataa
     5401 tgccttatgg catgaacgcg atatttctca ttcaagtgta gaacgcataa ttttacctga
     5461 ttcctgcatt ttgatccatt atatgttaaa caaaacatgt tcgctaatcg aaaatttagt
     5521 tgtttatcca gaaaatatga aaaaaaactt ggaactgacc aaaggtctgg tcttttctca
     5581 agccttactt ttgcatcttg ttaaaagtgg tttaacccga gaaaaagctt atgccttagt
     5641 tcaacaggaa gctatgaaga gttttgaaac caatactaat ttcctggata atgttcttgc
     5701 caataaagaa attacaaccg cacttgcgga aagtgaaatt cgagatatct tttcttatga
     5761 acgctatctg cgtaatgtgg attatatttt taggcgctgc ggaattttgt aacaaaggag
     5821 ctaggttatg aaacactatt ttcttatcct cattgcttcg ataatatgtg ttcctattct
     5881 gctttccgga aaagaagaaa cagaccgcat tatcggctat tggcttaccg gtgataaagc
     5941 aactaaaatt gaaatatacc aaaatgaaga tggtactttt gccgggaaga taatttggct
     6001 gcaagaacct aacgacgcaa aaggaaaccc taaaaaagat attaataatc ctgatgtaaa
     6061 attgcaacaa caacctctga ttgatttaat tgtcctatct gattttgtta gagaaaatag
     6121 aaacaaatat aaaaacggga aatattacga gccgactaca gggaaaacct attccgctaa
     6181 aattgaactt attaacaata acacaattac aatacgctat tttatcggtg ttcctactct
     6241 tgggagaacc gatacctgga tacgcagctc taactgatta attgaaccta atcaatgcat
     6301 ttacgaccat atagattcac gatgaaatca cgaatagatg taatttaatt gacttgaatt
     6361 tccggatgcc attctcccta aagcaatcga cacagaatat ttatagtaat atgaccattg
     6421 attaactagg agcaaggaca ttatctcgat gatttttagc taaattagag aaataatcac
     6481 aaaataaagg tctaattttt ctcttttttt cattttcccc tccccaaaac tctcaaattt
     6541 gttcattgtt atgtgcaatg atcttttcaa gctattatag catatagcgg taactgcata
     6601 atttggctgt aagaagagga actgcaggat gtcgttcttc cacaatcttc attttaaagg
     6661 ctttatatgt gcttctttga gttacttttt tatccatttt tcttctcctt gattttggat
     6721 cctgtgtcaa cttttttagg acgcctcaca aacaacccgt actcctctcc tcctcctctc
     6781 ctcttcctct cctcttcaac ctatagttgt ggatgagtga agaaggggtg gatgatgtgt
     6841 tgtatctggg ttacccagca agaaaagtaa ggattagagc aagccaacta tttatatgtt
     6901 gtaaatcttc cgggtgttag taataatcaa tggcttcgca ttatcgatct taatattctt
     6961 cttatcttga tgaacaattc actatatatg ggaagtgata gtatctcaaa ggttggctaa
     7021 aaaacagggg acaccaattt gatatgtgca acagttctag ttatattaac tgagcaagat
     7081 agcagctatc aggcaagaac atatagataa gtgcatccgt tttgcttatc atacttatta
     7141 tgcaagataa tgaatgagaa tttaaacttg gggaaatatc aagcttaaag caaaaacccg
     7201 gctgaaaaac cgggtttttg atattttcta tgtagtatac agtttacatt aatatgttga
     7261 ggttgcttga gtaattagtt gattaactct tgctatacga ttattgatat cagtgatagc
     7321 ttcttcactt gttgcacgga cttttgcatt gtttagatag tccagagctt ttctgaaatt
     7381 gctaacggct gagcttttag cattatcgcg ttgatttttt aaagttttag ctttagtccc
     7441 aacagctttg gctgcttgct tttccaaatc gatgaagtgg ttataatcat tagttccttt
     7501 actttggctg atagttgcca aataaacata aggcaaatag agagtaggat cttttttcag
     7561 agcttgtaag gcataagttt ctgcttcctg gtatttgttc tgtccgaggt agatagcagc
     7621 catattcata taagctaagg cgttatcagg gtgctgtttt attaaagcac ttaaagtatc
     7681 aatagctttt ttattaatgg aagcaacaaa tgcaggatcg gtagattctg atgctttcaa
     7741 acgatataaa ttaaccgcat ttagataagc cattgtattc tgggggttat ttttaataat
     7801 tgtctcatac ttggaaatag catcatccaa tcgattagct ttttgatagg caacggcaag
     7861 gcggttggaa atcagttcgt tatcaggaaa tttatcaaat gcttaccaaa accatcatac
     7921 aaaaaaagaa aaa
//
LOCUS       DAORHB010000051         7775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_9003_length_7775_cov_6.191451, whole genome shotgun sequence.
ACCESSION   DAORHB010000051 DAORHB010000000
VERSION     DAORHB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9003_length_7775_cov_6.191451"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKM80_02615"
     CDS             complement(<1..439)
                     /locus_tag="PKM80_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV92830.1"
                     /translation="MNTPKRVYLFGNGKAEGRADMKNLLGGKGANLAEMNLIGVPVPP
                     GFTITTEACIEYNKLGAAKLREILDNDVHLAIKHIEDIMHMQFGSVENPLLVSVRSGA
                     RASMPGMMDTILNLGLNDTTIQGLIKKTNNERFAWDSYRRFVQM"
     gene            complement(830..1465)
                     /locus_tag="PKM80_02620"
     CDS             complement(830..1465)
                     /locus_tag="PKM80_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HNV92831.1"
                     /translation="MKKIIYIAFGLMLLNLCFAQESPVTKYLQNQNLDTYKQAVDYLV
                     KMDTDGETGMQAKLNLAYISNLESLRLLDLAKENLDNLKPGERFALANLFLEMDKYPE
                     AIEIYDLLNEATPLWSCPWRHKGEALYKSGNFESAKIALGKAIETNEEHYDAYIWYAK
                     TLYELKEYKQALTALETAFNLTEENEESEFDMVLSEEELNQLYQELQKLAK"
     gene            complement(1481..1762)
                     /locus_tag="PKM80_02625"
     CDS             complement(1481..1762)
                     /locus_tag="PKM80_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNV92832.1"
                     /translation="MFTINEYFDGKVKSIAFQNKQGNFTVGVMDIGEYEFGTNTIERM
                     TVISGELIVRLPEKENWQKFCKGETFTVAAHQKFHLKVEEPSAYLCQYE"
     gene            complement(1833..1907)
                     /locus_tag="PKM80_02630"
     tRNA            complement(1833..1907)
                     /locus_tag="PKM80_02630"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1873..1875),aa:Asn,seq:gtt)
     gene            2483..3232
                     /gene="lptB"
                     /locus_tag="PKM80_02635"
     CDS             2483..3232
                     /gene="lptB"
                     /locus_tag="PKM80_02635"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNV92833.1"
                     /translation="MDKHKIRAQNLVKIYGKRAVVNDLSLEMSQGEVVGILGPNGAGK
                     TTTFYMIIGLAKPNNGKVYYDDKDISHKPMYKRARLGLGYLAQAPSIFAKLTVEENVL
                     AILQTLKLSRKEQKNRLEEALAELNLTLVAKQKAYTLSGGERRKLEITRALVTNPTFL
                     FMDEPFAGIDPIAVADIQDIIEKLRNKDIGIMITDHNVLETLKIVNRAYIIYEGKIIV
                     SGTSLELINDEKAKELYLGDRFLQSPFEQRL"
     gene            3229..4686
                     /gene="rpoN"
                     /locus_tag="PKM80_02640"
     CDS             3229..4686
                     /gene="rpoN"
                     /locus_tag="PKM80_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNV92834.1"
                     /translation="MTAIDQYLSLKQKQELALKPKMLQSLKMLALPILELESYIKQEL
                     INNPLLELREEKEEEDLDESKFEQSIPESPTPIEDSEISEEHIDTLAEARELTEILDN
                     WNEYHSGSENWSADIEHNHTESLIRYEENGKVKFLEQLYPYDLPEDEVDFITEIVDSC
                     DSYGFLPKGYNIINLAKKYHINAERAEELHNLVLSFSPKGISARNITECLLAQLTDEQ
                     KENRIIMGLISDQFENLIHRRYQKIASYFGVSEDTIFAAKEQISKLDPKPGLRITTTN
                     AAYVYPDVTMKLIDGEYEIIINDNITPNIIISPQYRRMISRGYFDKESMQYVRERINS
                     AKFLIKSIYMRMRTLERVSRSIIKHQHDFFYLGTGQMHPLTYAVIARDLGVNESTISR
                     VVKYKYAETPYGIYAFKDFFTSTAGYDDDYQSISRQKVKTHLIRLIETENKKKPYSDQ
                     ELVDLLKEEGLNISRRIITKYRDELGILNSHLRRK"
     gene            4687..6096
                     /gene="lpdA"
                     /locus_tag="PKM80_02645"
     CDS             4687..6096
                     /gene="lpdA"
                     /locus_tag="PKM80_02645"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNV92835.1"
                     /translation="MLYSQVIIIGGGPGGYEAAIRLSQYEIPCLVIEKERLGGTCLNW
                     GCIPTKALVKSAELYSEMLSADNFGLPKQNQEIEYQNVWSRKNAVVEQLASGIEFLFK
                     KRNIPVVKATAISVAKQKENYIVTTLEGESYSCEYLILANGSEVKGLPGIEIDEKDIL
                     SSTGILKMQELPEELVVIGGGVIGCEFASVFSTFGVKVTIIEFLPRLIYQEEEEISKR
                     LAMQFKKSGVKVLTNKGVQNINKKGSRLELKLSDDTILETEKVLLSVGRKPVNNLEWK
                     QIEELKTEKGAVIVDDFMQTSLDKIYAIGDLTGKLQLAHTASKQGLFAVEHIASRIYP
                     EKYKVPAKPINYINIPRCTFTHPEIASVGYTQKEAEEIFKEVLTGKFPFSANGKAVAM
                     GNPNGFVKIIARKDTSEIIGMHIMGPNATELIAQGAIMIANQNKTADVESIIFAHPTL
                     SEAIMEAIEDLQNLSIHKI"
     gene            6116..6652
                     /gene="raiA"
                     /locus_tag="PKM80_02650"
     CDS             6116..6652
                     /gene="raiA"
                     /locus_tag="PKM80_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNV92836.1"
                     /translation="MQITITARHFELTKAIRDYVESACSKLNKYFDQIIAMHVVLALE
                     NSRNICELSLHASKFKLQSQAEEMDMYLAIDNAVEKMEAQIKKLKDRITDHQKRALKQ
                     QFETYSHATDFELEPKSHMRKTIKTKRVVTESLSLSEAVEKLEASKEDFLIFKNIESD
                     RINVLVTKDHNVYKLLEP"
     gene            6857..>7775
                     /gene="hprK"
                     /locus_tag="PKM80_02655"
     CDS             6857..>7775
                     /gene="hprK"
                     /locus_tag="PKM80_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNV92837.1"
                     /translation="MKEITVYDLFDTKKKDLALSLITEPETLNKKIGSPYLNRPGLAL
                     AGYLELFAADRIQVLGETEVRYLQSLTEEDLITRIRNMFKMDIPCIVISKGLTLPPVI
                     EYIANELNIALFSSRLSTINLIENLSRYLRDIFALEKTIHATLVDVFGLGILLTGKSG
                     IGKSECALDLVHRGHSLVGDDLITLRYIEDKLIGKPGREFGYFMEIRGVGFINAERVF
                     GIERTRKQKTIDLQIELMPWQENMDYERIGLVSNYAEHLGVKIPIIYLPVSPGKSVSV
                     IVEVAAMNIILKGVGYDAAEDFNRKVNEEI"
BASE COUNT         2601 a         1358 c         1458 g         2358 t
ORIGIN      
        1 acatttgcac aaaacgacga tagctatccc aggcaaacct ttcattattg gtttttttaa
       61 ttaagccctg aattgtagta tcattcaatc ccagattcaa tatcgtatcc atcattccag
      121 gcattgaagc acgtgctcca gaacgcactg aaaccaaaag tggattttca acagagccaa
      181 attgcatatg cataatatct tcaatatgct ttatagctaa atggacatcg ttatctaaga
      241 tctctctaag tttggccgca cccaatttgt tgtattctat acaggcttct gtagtaatag
      301 taaatcccgg aggaaccgga accccaatta aattcatttc agcaaggttt gcaccctttc
      361 cgcctaaaag atttttcata tcagcacggc cttcggcttt tccatttccg aagagataaa
      421 cacgcttagg tgtattcatt tatcctccct attattatcg tcttatgata tattatatgt
      481 tttgtcagat tcttcgatat ggcaaatttg tcaactgctt tcttggatta tggatatatc
      541 caagtaaaaa accagagatt cctgtaaagt tcattatatt cagcataatt ataggtctaa
      601 aaaggtattt actgctattt cttttaagct catagatttt ggtatttttc tcctttttcc
      661 tattattcat aattttttgc tctttgcaga aaggaaaaga aggcaatagg gggctatata
      721 aaaaaaggaa atatagaata tagggcatac tccatatttc ctaaaaaaaa ctgttcgctg
      781 atgagtaaaa ttgcaatgca gctaaacagc ttcaggatag ttatccagct tatttagcta
      841 atttttgcaa ttcctgataa agctgattta attcttcttc tgaaagcacc atatcaaatt
      901 cgctttcttc attttcttca gttaaattaa aagcagtttc caaagcagtt aatgcttgtt
      961 tatattcttt taattcatag agagttttgg cataccagat ataggcatca taatgctctt
     1021 cattggtttc aatagctttt cccagagcaa ttttggctga ttcaaaattc ccacttttat
     1081 acagtgcttc acctttatga cgccaggggc agctccacaa aggggttgct tcattcagaa
     1141 ggtcataaat ttcgatcgct tcaggatatt tatccatttc cagaaataga ttagccaaag
     1201 caaagcgttc acctggtttc aaattatcca ggttttcttt ggccaaatcc agtaaacgca
     1261 aggattctaa attgctaata taggcaagat tcaattttgc ctgcattccg gtttcaccat
     1321 ctgtgtccat ctttaccaga taatctactg cttgtttata agtatccaaa ttttgatttt
     1381 gcagatattt ggttactgga gattcctgtg caaaacatag atttaacaac atcaagccga
     1441 aagcaatata gataattttt ttcattgtgg ttttactccc ttattcatat tggcaaagat
     1501 aggcacttgg ttcttccact tttaaatgga atttttgatg agctgctacc gtgaaagttt
     1561 ctcctttgca gaacttctgc caattttcct tttcaggtaa gcgtacaata agttctccgg
     1621 aaataactgt catcctttca atagtattag taccgaattc atattcacca atatccatca
     1681 ctcctacagt gaagttaccc tgcttatttt gaaacgcaat ggactttact ttgccatcaa
     1741 aatactcatt aatagtgaac atatatgtct ccttgttgaa tatagtttta ggcacataat
     1801 ctaaaaaatc caaaaatcat atataaaaaa agtggctccg gaagaggggt tcgaaccccc
     1861 gacctagtgg ttaacagcca cccgctctac cgctgagcta ttccggaaca caattcatta
     1921 taccttttct tggagagccc tttttttgtc aaccagaatt tttagtttgt attttctgaa
     1981 aggttttaag tatatcccta ttggcagagt attggattat ttaccatagg tgaagcaaga
     2041 ttttactact tttcggatac tgacattttg catatttact tcagaaattg ttctgctaaa
     2101 gaatcccggt ttaaactgcc tcattttaaa ccttccttag ttatttgtgg tagagctgcc
     2161 gtggtgttct tcggcaaaaa cccacagagg tacggtggca tctgcatggt agatcattaa
     2221 gcaagaatgg gataaaatta tatagattct ccaggttagc tgtggcaaaa ttgtgcccag
     2281 caaaaacaga taggtatatt aatccactta ttcttcctta tccgacgata ctataaaata
     2341 aacaggtggc aagatataaa gggtaagttt ttggcattaa gtaaattcgc tactgattac
     2401 agttagaaat gagcttagca aaaatcaatc catatgcaag tatttcttga caaaaaaatg
     2461 gctttcgttt aagtataaaa atatggataa gcacaaaatt cgagctcaaa acctcgttaa
     2521 aatatacggt aaaagagccg tagtaaacga cctatctttg gagatgagtc agggtgaggt
     2581 tgtaggtatt ttaggcccta atggggctgg taaaaccact actttttata tgatcatcgg
     2641 tttagcaaag cccaataatg gtaaagttta ttatgatgat aaggatatct ctcataagcc
     2701 aatgtataaa agagcgcgtt tgggcttggg atatctggct caggcacctt ctatttttgc
     2761 taaattgacg gttgaagaaa atgttttggc gattttacag acccttaagc taagccgtaa
     2821 agaacagaaa aatcgtttgg aagaagcttt agctgaatta aatttgactt tagtggcaaa
     2881 acaaaaagca tatactcttt ccggagggga gcgaagaaaa ctggaaatta ctcgtgcttt
     2941 agtaaccaat cctacttttc tttttatgga tgaacctttt gcaggaattg atcctatcgc
     3001 cgtagctgat attcaagata taattgaaaa actcaggaac aaagatattg gaataatgat
     3061 taccgaccat aatgtacttg aaactttgaa aattgtaaat cgggcttata ttatctatga
     3121 aggtaagatt attgtttcag ggacatcctt ggaactgata aacgacgaaa aagcaaagga
     3181 gttatatctg ggcgaccgat ttctgcaaag cccttttgag caacgactat gactgctatt
     3241 gatcaatact tgtctctaaa gcaaaaacaa gaattggcct taaagccaaa aatgctgcaa
     3301 tcattaaaaa tgcttgcttt gccaattctg gaactggaaa gctatataaa acaagaattg
     3361 atcaataatc ctttattgga actacgcgaa gaaaaagagg aagaagacct tgatgaaagt
     3421 aaatttgaac agtcgattcc agaaagccct actcctattg aagatagtga aataagcgaa
     3481 gaacatattg atactttagc ggaagcaaga gaacttacag aaattttgga taactggaat
     3541 gaatatcatt ccggaagcga aaactggtct gccgatattg aacataatca cacagaatct
     3601 cttattcgct atgaagaaaa cgggaaagta aaatttctgg aacagttata tccctatgat
     3661 ctacctgagg atgaagtaga ttttattact gagatagtag attcctgtga tagttacggt
     3721 ttcttaccca aagggtataa cataattaat ctagctaaaa aatatcatat aaatgcagaa
     3781 cgcgccgaag aactacataa tcttgttctt tcttttagtc ccaaaggtat atcagctcgc
     3841 aatataactg aatgtttact agctcagctt actgatgaac aaaaagaaaa cagaattatt
     3901 atgggtttga tatctgatca gtttgaaaat ctgattcatc gtcgatatca aaaaattgct
     3961 tcttacttcg gggtttctga agatacaata tttgcggcta aggaacaaat ttccaagctt
     4021 gatcctaaac ccggtttacg gattacaact accaatgcgg cttatgttta tcctgatgta
     4081 actatgaaac tgattgatgg tgaatatgaa attatcatca acgataatat tacgccaaat
     4141 ataattatca gcccccaata tcgaagaatg attagccgcg gttatttcga taaagaatca
     4201 atgcaatatg ttagagaacg cataaattcc gctaagttct taattaaatc catctatatg
     4261 cgaatgcgaa ctttggaaag agtttcccgt tctataataa aacatcaaca tgatttcttc
     4321 tatttaggca caggacaaat gcatccttta acctatgctg ttattgctag agacctggga
     4381 gtaaatgaat ctactattag cagagtagta aaatataaat atgcagaaac gccttacgga
     4441 atttatgctt ttaaagattt ctttacttct actgcaggtt atgatgatga ttatcaaagt
     4501 atttccaggc aaaaagtgaa aacccattta attcgtttaa ttgaaacaga aaataagaaa
     4561 aaaccctata gtgaccaaga actggtggat ctccttaaag aagaaggact taatatctcc
     4621 agaagaatta taacaaaata ccgggatgaa ttgggcattt tgaacagtca tcttaggaga
     4681 aaataaatgc tatacagtca agtgattatc attgggggag gccccggtgg ttatgaagct
     4741 gccatccgtc tttcccaata cgaaattccc tgtttggtta tcgaaaaaga acgcttagga
     4801 ggaacctgct taaattgggg ctgtattcct actaaggcat tggtgaaaag tgcagaatta
     4861 tattctgaaa tgctttctgc agataatttt ggcttgccta aacagaatca ggaaattgaa
     4921 taccaaaatg tttggagccg caaaaacgct gttgtggaac aattagccag tggcatagaa
     4981 tttttgttca aaaagcgtaa tatccctgtt gtgaaagcaa ctgctatttc cgtagctaag
     5041 cagaaagaaa attatattgt aacaacctta gaaggagaaa gttacagctg cgaatattta
     5101 attttggcca acggtagtga agttaaagga ttgcctggaa tcgaaattga cgaaaaggat
     5161 atcctgtctt caaccgggat cttaaaaatg caggaattac ctgaagaact tgtagtaatt
     5221 ggtgggggag taattggttg tgaatttgcc tccgtttttt ccacttttgg ggtgaaagtt
     5281 actattatag aatttctccc tcggttaata tatcaggagg aagaagaaat ttccaaacgc
     5341 ctggctatgc aatttaaaaa aagtggagtg aaggttttaa ccaataaggg agtgcaaaac
     5401 ataaacaaaa aaggaagccg cctggaactg aaactaagtg atgatactat tttggaaacg
     5461 gaaaaagtac tgctgagcgt tggtcgaaaa ccggtaaata atctggaatg gaaacagata
     5521 gaagaactga aaacagaaaa aggggcagtg atagttgatg attttatgca gacgagtttg
     5581 gataaaatct atgctattgg tgatcttaca ggaaagttac agcttgcaca tacagccagt
     5641 aaacagggtt tatttgcagt tgaacatatt gcttcaagga tatatcctga aaaatataaa
     5701 gttcctgcta aaccaattaa ctatataaat atccctcggt gcacttttac gcatccggaa
     5761 attgcttccg ttggctatac tcaaaaggaa gctgaagaaa ttttcaaaga agttttaaca
     5821 ggtaaatttc ctttttcagc aaatggtaaa gctgtggcaa tgggcaatcc caatggattt
     5881 gttaaaatca ttgcccgaaa agatacatcc gaaattatcg gaatgcacat tatgggtcct
     5941 aatgcaacag aattaattgc acagggagcc ataatgatag ccaatcaaaa taaaacggca
     6001 gatgtcgaat ccattatttt tgcacatcca actctttcgg aagcaataat ggaagcgata
     6061 gaagacctgc aaaatctatc aattcacaaa atctaataag aaggaggaat cccaaatgca
     6121 gatcacaatt acagcccgtc attttgagct caccaaagct atcagggatt atgtggaatc
     6181 ggcttgttca aaattgaata agtacttcga tcaaattatt gcaatgcatg tagttcttgc
     6241 tttggaaaac agtagaaaca tctgtgaact ttctttgcac gcatccaaat ttaaacttca
     6301 aagtcaggcg gaagaaatgg atatgtatct tgccatcgat aacgccgtag aaaaaatgga
     6361 agcacagata aagaaactaa aggatcgcat tacggatcac cagaaacgtg ctttaaaaca
     6421 acagttcgaa acatactctc atgccaccga ttttgagttg gaaccaaaga gtcatatgcg
     6481 taaaactata aaaactaaac gcgtagtaac tgaatccctc agtttgtctg aagccgtaga
     6541 aaagctggag gcaagtaaag aggatttctt gatttttaaa aacatcgaat ctgatagaat
     6601 aaatgttctg gtaacaaaag accataatgt ttataagttg ctggaaccat agttattaat
     6661 tgctgaatcg gataatcagg aaagaggaag agcaaaatca aggactaata aactcggctg
     6721 tataattata cttcgggttt aacgctataa atacataatt atgcttttcc tcttttcctt
     6781 taagaaaata gataaaatgt aatatgccac acattatttg ctggctttta aatttaggca
     6841 aaaccgaggt acatcaatga aggaaattac cgtttatgat ctttttgata ctaagaaaaa
     6901 agacctggct ttatcattaa ttaccgaacc ggaaactttg aacaagaaaa tcggttcccc
     6961 ttatcttaat cgtcccggtt tagctctggc tggttatctg gaattgtttg cagccgatag
     7021 aattcaagtt ttaggggaaa ccgaagttcg ctatttacaa agcttaacgg aagaagattt
     7081 aatcacccgc atccgaaata tgtttaaaat ggatatcccc tgtattgtaa tctccaaggg
     7141 tcttactctg ccaccagtta ttgaatatat agctaatgaa ctaaatattg ctctgttttc
     7201 cagtcgactc tctacaatta atctaatcga aaatctatct cgctacctaa gagatatttt
     7261 tgccctggaa aaaacaattc atgcaactct cgtagatgta tttggcttag gaattttgct
     7321 aacaggtaaa agtggcatcg gaaaaagtga atgtgctttg gatcttgtac atcgtggaca
     7381 cagcttagtt ggtgatgatt taataaccct gcgctatata gaggataagc tgattggaaa
     7441 accaggacgc gaatttggct attttatgga gattagaggt gtgggattca taaatgcaga
     7501 acgcgttttt ggaattgaac gcactcgtaa acaaaaaacc atagacctcc agatagaact
     7561 gatgccctgg caggaaaata tggattatga acgaattggt ttggttagta attatgctga
     7621 acatctgggg gtgaaaatac caatcattta cttacctgtt tctcccggaa aaagtgtttc
     7681 cgttattgtg gaagtagctg caatgaatat aattctgaaa ggtgttggct atgatgcagc
     7741 agaggacttt aaccgcaagg taaacgaaga aatcc
//
LOCUS       DAORHB010000052         7534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_9444_length_7534_cov_4.029148, whole genome shotgun sequence.
ACCESSION   DAORHB010000052 DAORHB010000000
VERSION     DAORHB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9444_length_7534_cov_4.029148"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKM80_02660"
     CDS             complement(<1..645)
                     /locus_tag="PKM80_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92838.1"
                     /translation="MKNKTLYLALVLLIVVAIIIVVLLISRKAPLNGPESIAYDDVSK
                     QFLVSNTLSKNIVSMSLNGQFTPFLKKGLQEPRGIIIYSHKLYVADKASIKVIDLDKA
                     EIIDTINIPGALMLNDLAFTKQGVIYTTDTAGNCVYVIDTLSNKIQKIANPLLQKPNG
                     IIYDMPRDQMFVVCFREKSPILAISTLDNSISIFMDSIYSDLDGIAIDDLGRIYI"
     gene            complement(642..2051)
                     /gene="cysS"
                     /locus_tag="PKM80_02665"
     CDS             complement(642..2051)
                     /gene="cysS"
                     /locus_tag="PKM80_02665"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNV92839.1"
                     /translation="MLLYNTQTRKKEKFIPITPGKVGIYACGPTVYNYFHIGNARAFI
                     FFDVVRRYFEYQGYEVTYVQNITDIDDKIIAQANEENTSFHMVADKYTRAFLEDIAAL
                     GINKPTYQPKATEVMNDIIAFIAALEQKGFAYSVEGDVYFATEKLPSYGCLSGKKMEE
                     QLAGARVVENPNKHHPADFTLWKKGKPGEPVWDSPWGKGRPGWHTECVVMSKKYLGDT
                     FDIHCGGIDLIFPHHENELAQAMAFSDKPLSNYWMHNGFLNVDGEKMSKSLKNFFTAR
                     DILAHYDAEAIRFFFLSKHYRSPIDFSRELIEEAQRAVANLYSSLSEINYLDNVQNSP
                     QTMCGTAVELQNLHKEYITAMDDDFNTAKAISILFELNRKVKNTSSAFADRLAAAQKL
                     VELGSILGFFQNLEEQLSTFMPDLSKDLIELILSYRQESRRNKQWTVSDRIRDDLARL
                     GIEIKDTPEGVKWNLKEKK"
     gene            complement(2063..5896)
                     /gene="porU"
                     /locus_tag="PKM80_02670"
     CDS             complement(2063..5896)
                     /gene="porU"
                     /locus_tag="PKM80_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNV92840.1"
                     /translation="MRRLLLFTLLIFTITFLNAKLTIIEKSETEFLLEFKLEPWELVA
                     EGNYSRIVAENMDYNNNTGAPLIPYDEFKVGIPPSGNINYSLLNSNTTSITLPSKLLP
                     VPEIKKVDGISSYEYKISPEEYQLSRTEFITELPVSNFRGYNFIPFIIYPFAYDGNKT
                     LTLTTQASIIINISGQTNFRSLEPSDKAADVFLAQLINEQQTRNWRNNPRPAIHYAPF
                     ANSDWWVRIETNREGMYRINRSDLSGLPLNDIDPTTIRIFTTTGKVLGNNIDDIGAEF
                     KEIPIIISGEADHKFDPEDYILFYGSSRDGYEMNANVQDNQYYNPYSQNQVFWLSFGG
                     SFNGNPIRIQTITPPSSYDVSVTSTPAVKHIENEIYRREETGFDWYSQTLFGNSTAEY
                     NFTTDLTDIDLSGEQIVQLRLRQEDTGEGTNHRIQVYVNGWPIIYSSDPDVYDFTWSG
                     TWPFTIKRNTTHLRNGSNTITIKVSRTTTDNLYLDYIHLAYQQNLNKSNIQKQFSHTT
                     SLYPIPCKFNLSGDLSNILVFRSNGIYSVDKIPLTDSYFISEGTSSTSYFILKPNETY
                     NPALIRVMHPTDLTANISQVDNIIVSPSEFISHAQVLADKYWDYYQVRSKVILVDDIY
                     NQFNGGHIDPAAIRLALKYFYSNFTAPRISSLTLLGLGTIDWRNFSGSAATKNKMIVW
                     QNGFLTSDDYFGMINTDYYPELAIGRYPVKNTAEMNIMLQNFSNYTSNPSHGWWRNSM
                     IFLADDLTNGPSNYEYIHTQDIEDIGSVLNPSILADKIFAMEYEYDEFQNKPRARDDM
                     FKAINEGRLVFLYAGHGSFDKLGAEDYLNGATDMGRFANPDKLTLFIAASCDVSQFDY
                     WGFESLGQKVVLMNNLGAIASYAATRKSYAENNRPMMKYLMESLADNRNPLGYSIMDA
                     KIRYTGSNSNDAVYVLLGDPLIRVIPPERDSTMQVNLLEDNVVLHSRETAIINGSFST
                     PGLSGEAEVRIFDPIRKYSLGPNTNVSKKGNQLFRGRSEVTASQYNSAFIVPDDIISG
                     NKGLCVSYIWDEATKKDYTNYYFPLGFSDQAVDVDNPDTPQINIYLGHKDFRPGDTVG
                     TNTTLYADFYDSNGINITGSSGHNILLVIDNSLQPLAITDYFNYDRNSCTNGNITYPL
                     SNLSEGIHTIQVIAFDNFNMPAVATTHFVAKKQTELDIEQLLIYPNPLSKDTYITFIL
                     TSDAQVNIGIYTLRGKRIHNLKVYGKQGFNKIYFSGKDDQGDTLSNNTYFLKVQATSA
                     DGKKVEKTEKMVIYK"
     gene            complement(5896..6045)
                     /locus_tag="PKM80_02675"
     CDS             complement(5896..6045)
                     /locus_tag="PKM80_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92841.1"
                     /translation="MLTIIATVLDNVKLSLGLMVQGMLGIFIFMAIFYLLIYALERFI
                     KPKEK"
     gene            complement(6061..6522)
                     /locus_tag="PKM80_02680"
     CDS             complement(6061..6522)
                     /locus_tag="PKM80_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNV92842.1"
                     /translation="MKLTLLQTGKTKDKWLLEGIAEYQKRISAYSKFQIIDLPDESIK
                     NSGNANIVKAKEANTILKAIKPDDYVILLDEKGEPISSLAFADFLVNISDKNVVFVIG
                     GVFGVDENIRKRANKILSLSHFTFTHRLARLVLCEQIYRALMIINNRNYHN"
     gene            complement(6519..7430)
                     /locus_tag="PKM80_02685"
     CDS             complement(6519..7430)
                     /locus_tag="PKM80_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNV92843.1"
                     /translation="MFHNFDEMLSYVKQNCKGIAIIAGAQIESALEAAIMAKKGNLCN
                     SILVGDQAEINRLLQEIAPELMDSFEIIDTGSNLIKAAETAVALAREGRGDLILKGKM
                     ESSVILKAALDKDKGLKVSDVISDVFVYEHPEGLKIQTDGGINILPELKEKIAMVKNA
                     VQVAHSLGNPNPNVAMVCAIETVNPKMPATIDAALIAKMNERGQITGCKIEGPLAFDN
                     AVDKEAARIKGITSEVAGAADILIFPNIETGNVHGKMLTYYCHYRIAHVTIGTKVPIL
                     IPSRADSGETKMLCMALALATMAKSKK"
BASE COUNT         2350 a         1424 c         1276 g         2484 t
ORIGIN      
        1 aatataaatt ctgcctaaat catcaatagc tatgccgtct aaatcggaat aaatactatc
       61 cataaaaatg ctaatgctat tatccaaagt actgatagct aaaatggggc ttttttcacg
      121 aaaacagacc acaaacattt gatcccgagg catatcatag ataataccgt tgggcttttg
      181 cagcaaagga ttagctatct tttggatctt attgctaagg gtatctataa cataaacaca
      241 atttccagct gtatctgtgg tataaataac tccctgtttg gtaaaagcta ggtcattgag
      301 catcaatgct ccagggatat ttatagtatc aataatttca gctttatcca aatctatcac
      361 tttaatagag gccttatcgg ctacatatag cttatgagaa taaataataa taccgcgagg
      421 ttcttgtaat ccttttttca agaaaggagt aaattgaccg tttaaactca tagaaacgat
      481 gtttttactt aaagtattgg aaaccaaaaa ttgtttacta acatcgtcgt aggcaatact
      541 ctcgggtccg tttaaaggtg cttttcggct aattagcaaa acaacgataa taattgctac
      601 tacaattagc agaacaagag caaggtagag agtcttattc ttcatttttt ctcctttaaa
      661 ttccatttga ccccttcggg cgtatcttta atttctatgc caaggcgagc aagatcatcc
      721 ctgattctat cagaaacagt ccattgttta tttcttcttg attcctggcg gtaggaaagg
      781 attagctcga tcaggtcttt agacaaatca ggcataaatg tacttaattg ttcttccaaa
      841 ttttggaaga agcccaggat agaacctaat tctacaagtt tttgagcagc agcaaggcgg
      901 tctgcaaaag ctgaagatgt atttttaact ttacggttca gttcaaaaag gatcgaaata
      961 gctttagctg tgttaaaatc atcatccata gctgtgatat attctttatg taaattttgt
     1021 aactctactg ctgttccaca catcgtttga ggtgagtttt gaacattatc cagataatta
     1081 atttccgaaa gtgagctata taaattggca acagctcgtt gtgcttcttc aataagttcc
     1141 cggctaaaat caatcggact gcgataatgc tttgatagaa agaagaaacg aattgcctcg
     1201 gcgtcgtagt gtgccaaaat atcacgagca gtgaagaaat tcttcaagct cttgctcatt
     1261 ttttctccgt ctacattcaa aaagccatta tgcatccaat aattggataa gggtttatca
     1321 ctgaaagcca ttgcctgagc cagctcgttt tcatgatgag ggaaaatgag atctattccg
     1381 ccgcaatgaa tatcaaatgt atcacccagg tattttttgc tcataactac gcattcagta
     1441 tgccaaccag gacgtccttt accccaggga ctatcccaaa caggttcacc tggtttacct
     1501 tttttccaaa gagtaaaatc ggcaggatga tgtttattag ggttttctac tactctggct
     1561 ccagcaagct gctcttccat ttttttacca gaaagacagc cataggaagg caatttctct
     1621 gtagcaaaat atacatctcc ttccacagaa tatgcaaagc ccttctgctc caaagcagct
     1681 atgaaagcaa ttatatcatt cataacttct gtagctttgg gttgataagt aggtttgtta
     1741 attcccaggg cagcaatatc ttccagaaaa gcgcgagtgt atttatcagc taccatatga
     1801 aaactagtat tttcttcatt tgcttgtgct ataattttat cgtctatatc ggtaatattt
     1861 tgtacataag taacttcata accctgatat tcaaaataac ggcggactac atcaaaaaag
     1921 atgaaagccc tggcattgcc gatatgaaaa tagttataaa cagtaggacc acaggcataa
     1981 attcctactt ttcctggagt tatgggtatg aacttttctt tttttcgggt ttgcgtatta
     2041 tacagtaaca tatcttttta tcctatttat aaatgaccat tttttcagtc ttttctactt
     2101 ttttgccatc tgcggaagtc gcctgaactt ttaaaaagta tgtgttattg gataatgtat
     2161 caccttggtc gtcctttcca ctaaaataga ttttattaaa gccctgctta ccatatacct
     2221 taagattgtg tatgcgttta cctcgcaagg tataaattcc aatgtttacc tgtgcatcag
     2281 aagtaagtat aaaagtgata taggtatcct tggatagggg attcggataa attagtagtt
     2341 gctcaatatc taattcagtt tgcttctttg ctacaaagtg agtagtggcc acagcaggca
     2401 tattaaaatt atcaaaagca attacttgaa tcgtatgtat cccttcgctc aaattagaaa
     2461 gtggataggt tatattgccg ttagtacaag aatttctatc atagttaaaa taatccgtaa
     2521 ttgctaatgg ctgcaatgaa ttatcaatta ctaaaaggat attatgcccc gaagatcccg
     2581 ttatgttgat accattggaa tcatagaaat cggcgtataa ggtagtattc gtcccgactg
     2641 tatcaccagg ccgaaaatct ttatgaccaa gataaatatt aatttgagga gtatcaggat
     2701 tatcaacatc cactgcttga tcactaaatc ccaaagggaa ataataattt gtataatcct
     2761 tttttgtggc ttcatcccat atataggaaa cacaaagtcc tttattccca ctgataatat
     2821 catccggtac aataaaagca gaattatact gggaagcagt aacttcactt ctacctcgaa
     2881 agagctgatt tccctttttc gatacatttg tattcggacc aagtgaatat ttccttattg
     2941 gatcaaaaat tcttacttct gcctctcccg aaaggcctgg tgtgctaaaa gaaccattaa
     3001 tgatagccgt ttccctggaa tgtagaacta cattatcttc taataaattt acctgcatag
     3061 tgctatctcg ttctggaggg ataaccctga taagaggatc tcctaaaaga acataaacag
     3121 catcattaga attacttcca gtataacgaa ttttggcatc cataatacta taacccaacg
     3181 gattgcgatt atcagccaaa ctttccatta aatatttcat catcggtcta ttattctctg
     3241 cataactttt acgggtagcg gcataagagg caatagctcc caaattattc atcagtacta
     3301 ctttttgtcc taaactctca aaaccccaat aatcaaattg agatacatca caggaagcag
     3361 caataaatag agtcagttta tccggattag caaatctacc catatctgta gctccgttta
     3421 aataatcttc tgcccccagc ttatcaaaag atccatgacc agcataaaga aaaacaagtc
     3481 gtccttcgtt tatagcttta aacatatcat ccctggcacg aggtttattt tggaattcat
     3541 catattcata ttccatagcg aatattttat cagctaatat gctgggattt agaacactac
     3601 cgatatcttc aatgtcttga gtgtgaatat attcatagtt agaaggacca ttagtaagat
     3661 catctgctaa aaatatcata gaattacgcc accagccgtg agatggatta ctcgtataat
     3721 tactgaaatt ttgcagcata atattcattt cagcggtatt ttttaccgga tacctgccaa
     3781 tggctaattc cgggtagtaa tctgtgttta tcatcccaaa atagtcatcg gaagtaagaa
     3841 aaccattttg ccagacaatc attttatttt tggttgctgc gctcccagaa aaattacgcc
     3901 agtcaattgt ccctaaaccc aaaagagtaa gagaggaaat tctgggtgca gtaaaattgg
     3961 aatagaaata ttttaatgcc aaacggattg ctgccgggtc tatgtgccct ccattgaact
     4021 gattatagat atcatctact aagatgactt tgctgcgtac ttgataataa tcccaatatt
     4081 tatcggcaag cacttgtgca tgagaaataa attctgaagg tgaaacaatg atattatcta
     4141 cttggctgat attagcagtt aaatctgttg ggtgcataac ccttattaat gcaggattgt
     4201 atgtttcatt tggtttcagt ataaaatagc ttgtagagct tgtaccttca gaaataaaat
     4261 agctatcagt tagtggaatt ttatccacac tgtagattcc attactgcgg aagacaagaa
     4321 tgttactaag gtctccactt aaattaaatt tacagggaat agggtatagg gaagttgtat
     4381 gggaaaattg cttttgaata ttgctcttat tcaggttttg ctgataagct aagtggatat
     4441 aatccaaata gagattatct gtagttgtgc gacttacttt tatagtaatt gtattgcttc
     4501 catttcttag atgagtagta ttgcgtttga ttgtaaaagg ccaagttcca ctccaggtaa
     4561 aatcatatac atctggatca ctactgtata taattggcca accatttaca taaacttgaa
     4621 tcctgtgatt agttccttct cctgtatctt cctgacgcaa acggagctga acgatttgtt
     4681 caccggaaag atcaatatcc gtaagatctg tagtaaagtt atattctgca gtactattgc
     4741 caaacagggt ttgactatac caatcaaaac cagtttcttc tctccggtag atttcatttt
     4801 ctatatgttt tactgcagga gtagaagtaa cagaaacatc ataactgctt ggaggagtta
     4861 ttgtttgaat tcttataggg ttaccgttga aactgcctcc aaaacttaac caaaaaacct
     4921 gattttggga atagggatta tagtattggt tatcctgcac attagcattc atttcatatc
     4981 catcgcgaga gctaccataa aataggatat aatcttccgg atcaaattta tgatccgctt
     5041 ctccagaaat aattatgggg atttctttaa attcagctcc aatatcatct atattattcc
     5101 ccaaaacctt tccagttgta gtgaaaatgc gaatagttgt cggatctata tcatttagag
     5161 gtaagccact aaggtctgaa cgattaattc tatacatacc ctcgcgatta gtttcaattc
     5221 taacccacca gtcactattt gcaaacggag cataatgtat agcaggacga ggattattac
     5281 gccaatttct tgtttgttgt tcatttatta attgtgccaa aaaaacatct gcagctttat
     5341 cgcttggctc taaactgcgg aagttagttt gtcctgaaat atttattatg atgcttgcct
     5401 gagttgttaa tgtaagggtt ttattcccat cgtaagcgaa gggatagata ataaacggaa
     5461 taaaattgta tcctctgaag ttggaaacag gaagctcggt tataaattca gttctgctca
     5521 gctgatattc ttctggtgag attttatatt cgtagctgct tattccgtct acttttttta
     5581 tttccggaac aggcaagagt tttgaaggta aagtgataga ggtcgtattg ctgttaagta
     5641 atgagtaatt tatgtttcca gaaggaggaa ttcccacttt aaattcatca taagggatta
     5701 gcggtgcgcc ggtgttatta ttgtaatcca tattttcagc cacaatacgg ctataattac
     5761 cctcggcaac caactcccag ggctcaagct taaattccag tagaaattct gtctcacttt
     5821 tttcgatgat tgttaactta gcattcaaaa aagtaatagt aaaaataagc aaggtaaata
     5881 ataataatcg ccgcattatt tctccttggg cttaataaaa cgctctaaag cataaataag
     5941 caaataaaat atggccataa agatgaatat tccaagcatt ccttggacca ttaatcccaa
     6001 ggataatttt acattatcca gaacagtagc gattatagtt agcattgttt ccccttctaa
     6061 ttaattatga taattacgat tattgattat catcaaagcc cgataaatct gttcacataa
     6121 aaccaatcgt gctaggcggt gcgtaaaagt aaaatgtgat aaggacaaaa tcttatttgc
     6181 tcttttccgg atattttcat ctacaccaaa aactccacct atcacaaaaa ctacattctt
     6241 atcagatata ttaactaaaa aatctgcaaa agcaagagag ctgattggct ctcctttttc
     6301 gtctaaaaga ataacataat catcaggttt gattgctttt aagatagtat tagcttcttt
     6361 tgccttaaca atattagcat taccggaatt tttaatactt tcatcaggta aatcaattat
     6421 ttgaaatttg gaataagcag agatcctctt ttggtattca gctattcctt ccagtaacca
     6481 tttatcctta gttttgcctg tttgtaaaag agttagtttc acttttttga ttttgccatt
     6541 gtagctaagg caagagccat acacaacatt ttagtttctc cactgtcagc gcgtgaagga
     6601 atcaatatag gaactttggt acctatagta acatgagcta ttctgtaatg acaatagtaa
     6661 gtaagcattt taccatgaac attgcctgtt tcgatattgg ggaatattaa aatatctgct
     6721 gcacctgcaa cttcagaagt gatacctttt atgcgagctg cttccttatc tacagcatta
     6781 tcaaaagcca aagggccttc gattttacac ccggtaatct gaccgcgttc attcatttta
     6841 gcaatcaatg ctgcatcaat agtagcaggc attttaggat taactgtttc tatggcacac
     6901 accatagcca cattcggatt aggatttcct aaggaatgag caacttggac cgcattttta
     6961 accattgcta ttttttcttt cagttcagga agaatattga ttccgccatc agtttggatt
     7021 tttaatcctt ctggatgctc atagacaaaa acatcagaaa tcacatcgct tacttttaat
     7081 cctttatctt tatctaaggc agctttcaaa attacagacg attccatttt gcccttcaaa
     7141 ataaggtctc ctcttccttc tcgggctaag gcaacagctg tttctgcagc ttttatcaaa
     7201 ttactgccgg tatcaataat ctcaaagcta tccataagct ctggagctat ttcttgcaat
     7261 agacggttta tttcagcttg atcaccaacc agaatactat tgcacaaatt accttttttt
     7321 gccattattg cagcttcaag agctgattca atttgtgctc cagcaatgat agctattcct
     7381 ttgcagtttt gttttacata gctcagcatt tcatcgaaat tatggaacat atttcactcc
     7441 ttatttataa tgtatataaa atagaaattt aatttctcac ggatcattat tgttcaatag
     7501 tgatattttt ccagaaatct atctacaaat tcat
//
LOCUS       DAORHB010000053         7392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_9727_length_7392_cov_5.694426, whole genome shotgun sequence.
ACCESSION   DAORHB010000053 DAORHB010000000
VERSION     DAORHB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9727_length_7392_cov_5.694426"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2525
                     /gene="pheT"
                     /locus_tag="PKM80_02690"
     CDS             <1..2525
                     /gene="pheT"
                     /locus_tag="PKM80_02690"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNV92844.1"
                     /translation="AKYLEIKENTKELEELLTFSGIEVEAVQNIPALPETVFSAKVIS
                     AEPVPKTDHLKLCLVDIGNYPYPEKTPEGYLQVICGAPNCKTGMMAVIALPGSTLADI
                     TIKKAKIKGIESNGMLCSEKELGISDNHSGIIELEPDTPIGRSVNELYELPDTVFELE
                     ITPNRPDLLGYIGIARDLSAKLGRPLKLPEIDIPVAKCRAETMPLELINKVPELCPRY
                     IARLFDKVKVKESPLWLKSALIKSGLRPINNLVDITNYVMLEYGHPLHCFDYEKLASL
                     PDKPGIPAIVIRQAYKDEPITTLDGKNYILEGEEMVIADGIKPSAIAGIMGGNISAIS
                     ENTKRIVLESAAFDPRSIRRTSYKLKISSDSSYRFERHLSECSAQDASLRASQFICEL
                     ADAELCEVVYDNYPVPEKSLILGVRPSRYAKVIGYELEEEKIKDYLQRLGLKFLRYGI
                     WKPGKITNISEIRELDSETLKQNNTALQNQEKAHYYLIPPKRVDLEREIDIIEEIARL
                     DGYDKAPVKTKPQHIMDRHSYRIKKVAVDYLVSRGFYETLNYSFSEPELMRKLGYQEG
                     DYELDMIFLKNPQSSNQSAMRTSLIPQLLQNLAYNLNRDEKNIKLFELGKTYHRQETI
                     SYEPYYLAAILTGLNKDEHWQDKASPINVFWVKGIVEELLSLLHLENWTVQEAKKPYL
                     SQSESAAYYLDNKELAYYGKLTAPVAENFDIDLIELKQDIWIVQFSMENLIVATHNLE
                     IQYQEIPRLPSVIRDISFLISEEIPYSKIVKTIAEVDDKMITDIIAFDEYRGKQIPNG
                     KRSLTLRLKIRDKEKTLTDERVDYLVDSVIKKLENTWKITMR"
     gene            2528..2854
                     /locus_tag="PKM80_02695"
     CDS             2528..2854
                     /locus_tag="PKM80_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92845.1"
                     /translation="MMESNVTNLQDKIQKLIDQYTAVTQKVEELEEINKQLAEENSQF
                     IAQLQNRDVATGEQEKKYKTLQDEYNILENRYKELQRILAGFETVAEGAIKKIDGILP
                     PLDNEK"
     gene            2857..3120
                     /locus_tag="PKM80_02700"
     CDS             2857..3120
                     /locus_tag="PKM80_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNV92846.1"
                     /translation="MKSVEVEIFGRRFRLRSDNPERTKHIAEEIDRQIEDLSKLYDNL
                     DFTKLLLLICLQQQEEVLTLGDKNQSLMSELERLNQMLSKITI"
     gene            3353..4921
                     /gene="rny"
                     /locus_tag="PKM80_02705"
     CDS             3353..4921
                     /gene="rny"
                     /locus_tag="PKM80_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNV92847.1"
                     /translation="MNINPYIALVIGVAVGFVIALFIYIIKRNSTFKLISQSTKIAEE
                     LERNAKIEAENAKKAALLEAREEWFKQKRILDEEIKERQKELRTQEKKYNERLSSLDK
                     RLDSLDKKETNLTEQERKLKQKEEELANKKTEYEQIITEQKNKLVEIAGLSRDEAIAL
                     LREELISQARQVAANDARLTLEQIKLDANQKAAEILSTAIQRIAVDHVSETTVSVISL
                     PSDEMKGRIIGREGRNIRTFEKVSGVDLIIDDTPEAVVLSCFDPVRREIARLSLEKLI
                     ADGRIHPGRIEEVVAKTTKEMEENLVKIGEKAVLETNIHNISPNLIKVLGRLHYRTSY
                     GQNVLQHSIESAWICGIIAAELGLDQELARRAGLLHDIGKAIDHEFDGSHAIIGANLA
                     RKNGEGKIIVNAIEAHHEEVEAISVYAALTQASDAISGARPGARREMLETYMQRLSKL
                     EEIANSVEGVSKSYAIQAGRELRIIVEPALVDENQTTLLATEIAASIEKQVQYPGQIK
                     VTVIRETRQIAMAK"
     gene            4921..5730
                     /locus_tag="PKM80_02710"
     CDS             4921..5730
                     /locus_tag="PKM80_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNV92848.1"
                     /translation="MKVLFFGDVYGKAGRRTVQQALPQLREEFNPDFVLANAENLADG
                     KGLTEKTLSQLFNSGVDAVTGGNHLWDRTESLEYLHSQKRIVKPLNYSEEAPGNPYLV
                     LSKGDLHLGIICLCGQLLMPPCDSPFAALDRFIKYSLSIDNEKQNNIPLPYPIILDFH
                     AETTSEKRAMGWFADGKISAVLGTHTHIQTADEEILPLGTAYITDVGMTGSHNSVIGV
                     KKEIIIEKFRTSVPNRFESSDEGLQVNAVFLVLDSETGFAEKITRIKRNID"
     gene            5736..6650
                     /locus_tag="PKM80_02715"
     CDS             5736..6650
                     /locus_tag="PKM80_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNV92849.1"
                     /translation="MEKILSGKPVAQLVNKATKTLITQYELKPTMLLIQVGSDPASNY
                     YVQNIINSGKKLQCNCEVIDLPEQITEQELLAIMEGANQNPDINGIMLQKPLPSHIKD
                     NIINMAIDPGKDLDCLHPLNLGKILSEENGFLPCTPAAVLFCMQYYGINPQGKNAVIL
                     GRSNVVGKPLANMLLWKKSFANATVIVCHSKTNNIAEITQKADILIAAIGKANFVTPE
                     MIKENAVILDVGINEEKGNDGQSIYVGDVNYNLCFDKASAITPVPGGIGRITTSILYF
                     NLIKACLEQKNINKSIDEYLDLIFKGIH"
     gene            6682..>7392
                     /locus_tag="PKM80_02720"
     CDS             6682..>7392
                     /locus_tag="PKM80_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92850.1"
                     /translation="MQVKSIKLLSIIMITLSAILLLSGCGKSGSRFANQPPTIRITSF
                     EGWDSTYVTAGYDTSLVYTFQQRIYWHATDPDGIITGYAFRVLDENNNPIPTPGYDYI
                     DTTGELTPDNLLAFGTGWVIHYMQGADHNIPLDDPSARRTIWTSQKYAVINFPSADAY
                     GNPIDKSSRFEVVAIDNRGAITPQPAWRNFCTHSDRPTCSITTTKGNPNNQDVGSGLK
                     LSFAMKDTDPFITPVPYQY"
BASE COUNT         2558 a         1317 c         1467 g         2050 t
ORIGIN      
        1 tagcaaagta tcttgagata aaagaaaata ctaaggagct ggaagagctt ttaaccttct
       61 ccggtattga agtggaagca gttcaaaata tacctgccct tccagaaact gtttttagcg
      121 caaaagttat tagtgctgaa ccagttccca aaacagacca tttaaaactc tgtttagtag
      181 atattggcaa ctatccctat cctgaaaaaa ctccagaagg ctatctacag gtaatatgtg
      241 gagcacctaa ttgtaaaaca ggaatgatgg ctgtaatagc tttaccggga tctactttag
      301 cagatataac tataaaaaag gcgaaaataa agggaattga atctaacgga atgctctgtt
      361 cggaaaaaga attaggtatt tcagataatc atagtggaat aattgagctg gaaccagata
      421 ccccaattgg gagaagtgtt aatgagctat atgagcttcc agatactgtt tttgaactgg
      481 aaattacacc caatcgtcca gaccttcttg gttatatagg tattgcgcgt gatttatcag
      541 ctaaattagg cagaccctta aaacttccgg aaatcgatat accagttgca aaatgcagag
      601 cagaaactat gccactggaa cttattaata aagtacctga actatgccct cgctatatag
      661 cgcgtttatt tgataaggtt aaagtgaaag aatccccgct gtggttaaaa agtgctttaa
      721 tcaaatcggg gttaagacca atcaacaatc tggttgatat taccaactat gtaatgttgg
      781 aatatggtca tcccttgcat tgttttgatt atgaaaaact tgcctccctt cctgataaac
      841 cgggaattcc agcaatcgtt attcgccaag catataaaga tgaaccaata actactctag
      901 atgggaaaaa ttacattctg gagggcgagg aaatggttat cgctgatgga attaaaccct
      961 ctgcaatagc aggaattatg ggtggaaata tttctgcaat cagcgaaaat accaaaagaa
     1021 ttgttttaga aagtgcagct ttcgatccaa ggtcgattcg cagaacttct tataaattga
     1081 agattagttc agattcttcc tatcgatttg aaagacattt aagtgaatgt tcagcccaag
     1141 atgcatcttt aagagccagc caatttattt gtgaattggc agatgctgaa ctctgcgaag
     1201 tagtttatga caattatcct gttcctgaaa aatccttgat cttaggtgtc cgacccagcc
     1261 gttatgcaaa agtaatcggc tacgaactgg aagaagaaaa aataaaggac taccttcaac
     1321 gcttgggact taaatttctg cgatatggta tttggaaacc aggaaaaata accaatattt
     1381 ccgaaatcag agagttagat tcggaaacat taaaacagaa taataccgcc ctgcaaaatc
     1441 aggaaaaagc acactactat cttattcccc ccaaaagagt ggatttggaa cgcgagattg
     1501 atatcattga agaaattgcc cgtttggacg gttatgataa agctcctgta aaaacaaaac
     1561 cacagcatat tatggaccgc cattcctatc ggattaagaa agtagcagtg gattatctgg
     1621 ttagtcgtgg tttttatgaa acgctcaatt acagtttttc agaaccggaa ttaatgcgta
     1681 agcttggtta ccaagaaggt gactacgaat tggatatgat ttttctgaaa aacccgcaaa
     1741 gcagtaatca aagtgctatg cgcacttctt taattcctca gcttttacag aatttagcct
     1801 ataacctgaa tcgggatgaa aagaatatta agctctttga actgggaaaa acttatcacc
     1861 gccaagaaac tatatcttac gaaccctatt atttagctgc aattttaacg ggactaaaca
     1921 aagatgaaca ctggcaagat aaagcaagcc ccataaatgt tttttgggtg aaaggcatcg
     1981 tggaagaatt gctatctctg ctgcatttgg aaaactggac tgtacaagaa gcaaaaaaac
     2041 cctatctttc acaatctgaa agtgctgctt attaccttga taataaggaa cttgcctatt
     2101 acggaaaatt aactgcaccc gtggcagaga attttgatat cgatcttatt gagctaaaac
     2161 aggatatctg gattgtgcaa ttttctatgg agaatctgat agtcgctact cataaccttg
     2221 aaatccaata tcaggaaata ccgcgcttgc catcagttat tagagatatt tcttttctaa
     2281 tctcagaaga aataccctat agcaaaattg taaaaacaat tgcagaagta gatgataaga
     2341 tgataactga tataattgcc tttgatgaat atcgcggtaa acaaattccc aatggcaaac
     2401 gcagcttaac attacggctg aaaattagag acaaagaaaa aacattgaca gatgaacgag
     2461 tagattatct tgttgattca gtgataaaaa agctcgagaa tacttggaaa ataactatga
     2521 ggtaaagatg atggaaagta atgttacaaa ccttcaagac aagatacaga aactgataga
     2581 tcaatatact gcagttactc aaaaagtgga agaactggaa gaaattaaca agcaactcgc
     2641 cgaagaaaat tctcagttca ttgctcagct tcaaaataga gatgtggcaa ccggtgaaca
     2701 ggaaaaaaag tacaaaacac tgcaggatga atataacatt ctggaaaata gatataaaga
     2761 actgcagaga atccttgctg gttttgaaac tgttgccgaa ggtgctataa aaaagatcga
     2821 cggaatttta ccgccgctgg ataacgagaa ataaatatga aatctgttga agtagagatc
     2881 tttggacgca gatttcgttt aagaagtgat aatccggaac gaaccaaaca tatagctgaa
     2941 gagattgacc gtcaaattga ggatttatct aagctatacg ataatctgga tttcactaaa
     3001 ctgctacttc tgatctgcct tcaacaacaa gaagaagttt taactttagg cgataagaat
     3061 caaagtttaa tgtctgaact ggaacgctta aatcagatgc tttccaaaat aactatatag
     3121 atagtgcctg cagaattcgt gatatgggtt gcataaaagc tattataatt ttgggtttcg
     3181 agccatgagt ggcatgcgcg ccggctggtt ccggatacat aaaactcaca aggtagattc
     3241 ccacttaaag cttggctttc gaatgacaag cgtacacggt tcatgcgggc aatattataa
     3301 ttcgctttgg gctaaaaccc gaagttaaat aaatttacat aaggaaataa ctatgaacat
     3361 aaatccttac atagcactcg taattggagt ggctgtcggc tttgtcattg cgctttttat
     3421 ttatattata aaacgcaata gcactttcaa acttatctca caatctacaa aaatagctga
     3481 agaactggaa agaaacgcta aaattgaagc ggaaaatgct aaaaaagctg ctctcttgga
     3541 agcccgagaa gaatggttta aacaaaaacg catcctcgat gaagagatta aggaacgcca
     3601 gaaagaactg cgcactcaag aaaagaaata taacgaacgc ttaagtagct tagataagcg
     3661 tctggattcg ctcgataaaa aagaaactaa cctcacagaa caggaaagaa aactaaaaca
     3721 aaaagaagaa gagcttgcta acaagaaaac tgaatatgaa caaatcatca cagaacagaa
     3781 aaataaatta gttgaaattg cagggctctc tcgagatgaa gctatagcgc ttttacgaga
     3841 agaattaatt tcacaagcca gacaagttgc cgcaaatgat gcacgattaa cgcttgaaca
     3901 aataaaactg gatgccaatc aaaaagctgc tgaaatacta tctaccgcta ttcagcgtat
     3961 tgctgtggat catgtttctg aaacaaccgt ctcggttata tctttacctt cagatgaaat
     4021 gaagggacgc attattggta gagaaggaag aaatattcgc acttttgaaa aggtttccgg
     4081 tgtagattta attattgacg atactcctga agccgttgtg cttagttgct ttgatcctgt
     4141 ccgtcgggaa atagccagat tatctttaga aaagcttatt gccgatggcc gaattcatcc
     4201 tggacgcatt gaagaagtag tagctaaaac taccaaagaa atggaagaaa atttagtaaa
     4261 aataggcgag aaagcagttc tggaaactaa tatccataat atttcaccta acctaattaa
     4321 ggttctggga cgcctacatt atcgaacaag ttatggccaa aatgttttac agcattcaat
     4381 agaatctgct tggatttgtg gaattatagc tgctgaactt ggattagatc aagaacttgc
     4441 tcgccgagca ggtctgttac atgatatcgg aaaagcaata gatcatgaat ttgatggctc
     4501 tcatgctata attggagcta atctagcccg taaaaatgga gaaggaaaga ttatcgtaaa
     4561 cgccattgag gctcaccatg aagaagtgga agcaatatct gtttatgcag ctttaactca
     4621 agcctcggat gctatttccg gagccagacc tggtgccaga agagaaatgt tagaaaccta
     4681 tatgcagcgt ttatctaagc ttgaagaaat agccaatagc gttgaaggcg ttagtaaatc
     4741 ctatgcaatt caagctggac gagaactccg gatcattgtt gagcctgctt tggtagatga
     4801 aaatcaaact actcttttag caactgaaat tgctgcctcc attgaaaagc aagttcaata
     4861 tccaggtcaa ataaaagtaa ccgtgataag agaaacgcgc cagatagcta tggcaaaata
     4921 atgaaagttc ttttttttgg cgatgtttat ggaaaagctg gtcggcgaac tgttcagcaa
     4981 gcattgcctc aattgagaga ggagttcaat ccggattttg ttttggccaa tgcagaaaat
     5041 cttgcagatg gtaaaggcct tacagaaaaa actctatcgc agctattcaa ttctggagta
     5101 gatgcagtta ctggaggcaa tcatttgtgg gatagaacag aatctttaga atatctccat
     5161 agccaaaaaa ggattgttaa acctctaaat tactctgaag aagcacctgg caatccttat
     5221 ttagttttat ctaaaggaga tctccactta ggaattatct gcctatgcgg acaattactt
     5281 atgccacctt gtgatagccc ttttgcagcg ttggataggt ttattaagta cagtttatct
     5341 atagataacg agaaacaaaa caatatccct ctaccctatc ccataatttt agattttcac
     5401 gctgaaacta caagtgagaa aagagctatg ggttggtttg cagatggaaa aattagtgca
     5461 gttttaggaa cccacactca tattcaaact gcggatgaag aaatattgcc cttgggaaca
     5521 gcttatataa cagatgtggg gatgactggt tcccacaata gcgtgattgg cgttaaaaaa
     5581 gaaattatta ttgagaaatt cagaacctct gttccgaatc gttttgaatc ctcagatgaa
     5641 ggtttacaag tgaatgccgt ttttctggtg ttagattcgg aaacaggatt tgctgaaaag
     5701 ataacacgga taaaaaggaa tattgattaa gtactatgga aaagatctta agtggaaaac
     5761 ccgttgctca attggttaat aaagcaacta aaactttaat aacacaatac gaactaaaac
     5821 caacaatgct cttaattcag gtgggaagcg atcctgcctc aaattactat gtgcaaaaca
     5881 ttatcaatag cggaaaaaaa ttacaatgta attgcgaagt gatagatctc ccggaacaga
     5941 taactgaaca agaattattg gctattatgg aaggagctaa tcaaaatccc gatataaacg
     6001 gaataatgct gcaaaaacct ctcccttcgc atattaagga taatattatt aatatggcta
     6061 tagatccggg aaaggacttg gactgcttgc atcctcttaa tttaggaaaa atactaagcg
     6121 aagaaaatgg atttcttcct tgtacacccg cagcagtact tttttgtatg caatattatg
     6181 gaattaatcc tcaaggcaaa aatgcggtta ttctgggtcg cagtaatgtt gttggtaaac
     6241 ctttagcaaa tatgctgctt tggaaaaagt catttgccaa cgccacggtt atagtttgcc
     6301 atagtaaaac aaataatata gcggaaatta cccaaaaagc tgatattcta attgccgcta
     6361 ttggtaaagc gaattttgtt actccggaaa tgataaaaga aaatgctgtc attttggatg
     6421 ttggcataaa tgaagaaaaa ggaaacgatg ggcagagcat ttatgttggt gatgtaaatt
     6481 ataacttatg ctttgataag gcatcagcga ttactcctgt ccctggtgga attggacgaa
     6541 taactacttc tattctctat ttcaacctca ttaaagcttg ccttgagcaa aaaaatataa
     6601 ataaaagtat tgacgaatat cttgatctga ttttcaaggg tattcattag aacctacaaa
     6661 ttaagaagtt tgggaggaat gatgcaagtt aagagtatta aactcttgtc tatcattatg
     6721 ataaccttaa gtgcaatatt gttgctatcg ggatgtggaa aatctggtag ccgttttgcc
     6781 aatcaaccac caacaataag aataacctct tttgagggtt gggatagcac atatgtaacc
     6841 gctggttatg atactagcct tgtttacaca ttccaacagc gtatctactg gcatgctact
     6901 gatcctgatg gtataataac cggttatgct ttcagagtgt tagatgaaaa taacaatcct
     6961 attcctactc cggggtatga ttatattgac acaactggag agcttactcc tgacaatctt
     7021 ctagcttttg gaaccggatg ggtaattcat tatatgcaag gtgcagatca caatattcct
     7081 ttggatgatc cttcagcacg ccgcactatt tggacttccc aaaagtatgc tgtaatcaat
     7141 ttcccttctg cagatgctta tggaaatccc atagacaaat caagccggtt tgaagttgta
     7201 gcaatagata atcggggagc tataactcct caacctgcct ggcgtaattt ttgtactcat
     7261 tccgaccggc ctacctgttc aattactaca accaaaggaa atccaaataa ccaagatgtc
     7321 ggttcgggct tgaaattatc cttcgcaatg aaagataccg atccttttat tacaccagtt
     7381 ccctatcaat at
//
LOCUS       DAORHB010000054         7277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_9974_length_7277_cov_5.497092, whole genome shotgun sequence.
ACCESSION   DAORHB010000054 DAORHB010000000
VERSION     DAORHB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9974_length_7277_cov_5.497092"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /gene="csm2"
                     /locus_tag="PKM80_02725"
     CDS             <1..393
                     /gene="csm2"
                     /locus_tag="PKM80_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HNV92851.1"
                     /translation="RNTTPDRGGAKYGPFFKEDGSLNLAWVGEKAQRISQEISDARYG
                     LTATGLRNFYNEFLRIRNLPASHSEEKKILIKLLIAKVNYKKLNNKAPDIFAKFITEL
                     ITEVGDDLDKFEKSCLIMEAVVGFNPRK"
     gene            413..1111
                     /gene="csm3"
                     /locus_tag="PKM80_02730"
     CDS             413..1111
                     /gene="csm3"
                     /locus_tag="PKM80_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HNV92852.1"
                     /translation="MLTTKIITGKILLKSGLHIGGNKDTMQIGGIDNPVMKNPLTNMP
                     YIPGSSLKGKMRFLLEHYLNYVKGDGKIPGLTVDGKVNKIAIIFGHLEHTENSSDTKP
                     TRIIFSDCNIIGARDETGNLIEDLEYLKEKMLSNFVEAKTEVAINRITGTAQGGALRT
                     IERVPAGVVFDLHIVLKNYEESDESNLEIIKKGLKLLENDALGGSGSRGSGRIEFLDL
                     KVDGASIDLNSVQL"
     gene            1121..2134
                     /locus_tag="PKM80_02735"
     CDS             1121..2134
                     /locus_tag="PKM80_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92853.1"
                     /translation="MTTWKISWKPLSSLSTPLQSDTIFGHLCWAINYLWEEKDKNKLT
                     DFLSALQETPCLVLSSAFQEGHLPKPSIPIGNKHLDEVKKNLKKCKELKEITDLEISI
                     VLKRIKKDNWIDRKALTDNEYVYNVSEELISKAQDTALKILESRGTRIHSPKKEEGMI
                     EFHNMIDRITGTTSGSGELFASPTVFYKDICFESWLDTDYFNESEIKDIFHFIEQNGF
                     GKDKNTGKGKFEITVEEFHWQDCPKNNAYLNLSNMVPCASDSPIASYNSKTKFGKVGG
                     DYATSKTPFKYPIYIIEPGAVFFAEVRDKKPIGSLLRNIHPTEDIVQNLYSYNIPIMI
                     KGS"
     gene            2137..3240
                     /gene="csm5"
                     /locus_tag="PKM80_02740"
     CDS             2137..3240
                     /gene="csm5"
                     /locus_tag="PKM80_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HNV92854.1"
                     /translation="MKPLKYPFRISPITPFHIGNGTQYDPLNLIIKNGTAYFINQIEY
                     IRYLMEKDKKKLDRNLEVSNIKQLHKFFAESFDPNAKDTFYFSYPVKKDICDTYAKNM
                     ENLQNQGFIQAFIRSTLNMQPYIPGSSIKGAIRTAVLSNICSDKNKIIQDRNTDKADQ
                     KTQAVLLEYWNREKNSVDVPSDPFKFIKFEDIPWKNDWIRIFGVEITTPPVSASVRQN
                     PFHSGNQSEKEKKEPPIPVLMEVALSRIEWVVDSELTVLVTDKNNRGFNKIMPSGPND
                     IKPLIKMVNNYYQQQIDRESEYYKSMNPNAQKNYKAIKGLFDNLKDNECIIKLGMGTG
                     QNYCSYAVMNYQPKSRRMINSLPMGWMRLTFSI"
     gene            3249..4340
                     /locus_tag="PKM80_02745"
     CDS             3249..4340
                     /locus_tag="PKM80_02745"
                     /inference="COORDINATES: protein motif:HMM:NF021189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92855.1"
                     /translation="MNSIILSPVGTSIFTKGKFPSEINQFSNYKNLEDIPSDRRGIIS
                     SYIQEVHDLMLSSSLEDVKKRSAELNGIIHYYNDNLHNAQKDIHYLLPSDTYIGKSAC
                     EIIKCFLDKYIGSVCVLEIKDLQTYDCDRFQYALSDVVSQIMIIKRGLLPEQKFIFNL
                     TGGFKSILCFLQSLGMFYADETVYVFEYSKDLMRIPALPVKLVPYDYLKEYIVVFRRL
                     YNNLSVAEEEYDKIPESLILKLYGEPTLSAYGKIVWDNFRKDLYSEKLLDSISPKVKY
                     SEIFLKSTKDLGKDRYYNLNMRIDEVSCFMELKSKPNPASLDLKKLATPKGISTHEFD
                     AWADKDAKRVFCHYDQDILVLDTLLEGLH"
     gene            complement(4332..6395)
                     /locus_tag="PKM80_02750"
     CDS             complement(4332..6395)
                     /locus_tag="PKM80_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease"
                     /protein_id="HNV92856.1"
                     /translation="MSQLIISMGKTSYLIAEVVGFFNYEQYPLYYNHKSFETLKETRE
                     KNQLEPINTISMICTNDSFTSNKQAVEDWLKAQHLNIDLQFHILNQLKDISSAEDARI
                     MRNLIYSLVYNCYKQGNSNKLYLCLSGGRKTMSSDIQQAAYLFGCKAMIHILAEGDVS
                     FNLSAAPEDIEPAVINKINPVLYQKDIPASKLAELIEEEKVYFPEPKIKGNEYIYSDS
                     ETKFLNQVEELQTKQDNLTINYYTKLRYSEPSSNFQILHLLNPKKIQELKNNYINGID
                     NIKQDLNWLYKLPKTDLHCHLGGFADTEGLIKIAIANLKYSGNTLEEKKLERSTINAI
                     QKNDLNKLKAIWKTISTRENKVRWLLVSLYLRCFEGKEKLLEQVIWNNLLQEDNFVDI
                     GLENYEEIGDFQGSSLLQTEEALREICHQLKDDAKKNNLRYKELRCSPCNYTNKLKAT
                     EVVEILYDELIDEECVFRLIIIGSRHKTQRILKEHIDLCKNIKRENGKISDFICGFDL
                     AGPEEKTTLSLREKILPLLEDCIRITIHSGETTSVKNIWEAVYNLNADRIGHGLYLQD
                     DENLMLKLKENKTAIELCPSSNFQINSYRDYSINSTSKMRIYPLKQYLQKGIKACICS
                     DDPGISRTDISKEYLKAAKMTKGGLSKWNILTIIRNGFVSSFQTRENKQKLISEAEKD
                     IMKLV"
     gene            complement(6410..>7277)
                     /locus_tag="PKM80_02755"
     CDS             complement(6410..>7277)
                     /locus_tag="PKM80_02755"
                     /inference="COORDINATES: protein motif:HMM:NF033611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAVED domain-containing protein"
                     /protein_id="HNV92857.1"
                     /translation="ETIPIPIIQYQGEENELIRWQKALEQKVQENFSWFSYELLEDFY
                     GITTNNLAIFGNGILPFEKNAWQTLLKEQVKDKFNALEDKIMPKKVLWFYSGQISTLQ
                     IGIGALFGFKRAVSILQMEFSNTTYKEVFTLYGKENARQLKNVSFKKEDFQYIQSELQ
                     INDPQKNELGFIIYLGSHNPIGEAKAFCQKNLQVNNFLIIKAKQDQGVMETSQNWLPF
                     VQEINSALNAAREKNHWQRIHLFQTAPIALCMALGIAIGHFVPIDVYHYQFNASEPKY
                     RCMFSMDKILSL"
BASE COUNT         2343 a         1201 c         1283 g         2450 t
ORIGIN      
        1 cggaatacca cacctgatag aggtggtgca aaatatggac ccttttttaa agaggatggt
       61 tctttaaatt tggcctgggt tggtgaaaaa gcacagagaa tctcacaaga gatatctgat
      121 gcaagatatg gtcttacagc aacgggatta aggaatttct acaatgaatt tcttagaata
      181 cgcaatttac ctgcaagtca tagtgaagaa aagaagatat tgatcaaatt actaattgct
      241 aaggttaatt acaaaaagtt aaataacaaa gcaccggata ttttcgcgaa atttattact
      301 gaactaatta ccgaagtcgg ggacgacttg gataaatttg aaaagtcctg tttaattatg
      361 gaagccgtag taggtttcaa tcccagaaaa taaatttata aggagaacga aaatgttaac
      421 aactaaaata ataaccggaa aaatactgct gaaaagtggt ttacacattg gtggaaataa
      481 ggacacaatg caaattggtg gaattgataa tcctgtaatg aaaaatcctt taaccaatat
      541 gccctatatt ccaggttcct ctttaaaggg aaagatgaga ttcttgctgg aacattattt
      601 gaattatgta aaaggtgatg gtaaaatacc tggtttaaca gtagatggga aagtaaataa
      661 aattgcaata atctttggac atcttgagca taccgaaaat agtagtgata caaaacctac
      721 ccgtattatt ttttccgatt gtaatataat tggtgcaaga gatgaaactg gtaatttaat
      781 tgaggatctt gaatatctga aggaaaaaat gctctccaat tttgtagaag caaaaacaga
      841 agtagcgatt aatagaataa ctggcacagc tcagggagga gcattaagaa ctatagaacg
      901 agttcctgca ggagttgttt ttgatttaca tattgttcta aaaaattatg aagaaagcga
      961 tgaaagtaat ctggaaataa tcaagaaggg cttgaagctt ttagaaaatg atgctttagg
     1021 gggatcaggt tccaggggca gtggtagaat agaatttctc gatcttaaag ttgatggagc
     1081 ttctatagat ttgaattcag ttcagttata ggaggtcgta atgactacct ggaaaatatc
     1141 ctggaaaccc ttatcttctc tttccacgcc tttgcagagc gatacaatct ttggccatct
     1201 ttgctgggct ataaattacc tgtgggaaga gaaagataag aataaactta cagatttcct
     1261 atctgctcta caagaaactc cttgtcttgt tttatcttct gcctttcagg aaggacattt
     1321 acctaaacct tctattccca taggaaataa acacttggat gaggttaaaa agaatctaaa
     1381 aaaatgtaag gaattgaaag aaataacgga tttggaaatt tccatagtgc tgaagagaat
     1441 aaaaaaagat aactggatag atagaaaagc tctcacagac aacgaatatg tatataatgt
     1501 gagcgaagag cttatctcga aagcacaaga cacagcactg aaaattctgg aaagcagagg
     1561 aacccgtatt cattcaccta aaaaggaaga aggaatgata gagtttcaca atatgattga
     1621 tcggataaca ggaactactt ctggaagcgg agaacttttt gccagtccaa ctgtgtttta
     1681 taaggatatc tgctttgaat cctggctgga tactgattat tttaatgaat ccgaaatcaa
     1741 agatatattc cattttattg agcagaatgg tttcggtaag gataaaaaca ctggcaaagg
     1801 taaatttgaa ataacagtag aagaattcca ttggcaggat tgtcctaaaa ataatgctta
     1861 tcttaatctt tccaatatgg tcccttgtgc ttcagatagc cctattgctt cttataattc
     1921 caaaactaag tttggcaaag tgggtggaga ttatgctact tctaagaccc ccttcaaata
     1981 tccaatatac attattgaac caggagctgt attttttgct gaagtaagag ataagaaacc
     2041 tataggttca ttattaagga atattcatcc tactgaggat atcgttcaaa atctatattc
     2101 atataatatt ccgattatga ttaaagggag ctaaaaatga aaccgctaaa atatcccttt
     2161 cgtattagcc ctataactcc ttttcatatt ggcaatggga cccaatacga tcctttgaac
     2221 ctcattatta aaaatggaac agcttacttc ataaaccaaa ttgagtatat ccgctatctt
     2281 atggaaaaag ataaaaaaaa attagataga aatcttgagg tatctaatat caaacaactt
     2341 cataaatttt tcgctgaatc gtttgatcct aatgccaaag atactttcta cttttcttat
     2401 ccagtgaaaa aagacatttg tgatacttac gctaaaaata tggagaatct ccaaaatcaa
     2461 ggatttattc aagcttttat tcgttctacc ttaaatatgc aaccctacat tccgggaagt
     2521 tctattaaag gagctattcg aacagctgtt ctctctaata tatgttctga taaaaataaa
     2581 attatccaag atagaaatac agataaagct gaccaaaaaa ctcaagcagt gttacttgaa
     2641 tattggaata gagaaaagaa ttctgttgat gttccatccg accccttcaa atttattaaa
     2701 tttgaagaca tcccctggaa gaatgattgg attaggattt ttggagtgga aattacaact
     2761 ccgcctgtat ctgcaagtgt tcgccaaaat ccttttcata gcggaaacca atctgaaaaa
     2821 gaaaaaaaag aaccacctat tcccgtgcta atggaagtag ctctctccag aattgaatgg
     2881 gtagtggatt cagaattaac tgttttagtt acagataaaa ataaccgcgg gttcaataaa
     2941 ataatgccat ctggtcctaa tgatattaaa cccttaataa aaatggttaa taactattac
     3001 caacagcaga tagatagaga atcagaatac tacaaaagta tgaatcccaa tgcgcagaaa
     3061 aactataaag ccatcaaggg tttatttgat aatttaaaag ataatgagtg tatcatcaag
     3121 cttggtatgg gaacaggtca aaattattgt tcctatgccg taatgaatta tcaacctaaa
     3181 agtaggagaa tgatcaattc tttgccgatg ggttggatga gattaacttt tagcatatag
     3241 gagaaaaaat gaattctatt atcctatcgc cggtcggaac cagtattttt actaaaggga
     3301 aattcccttc tgaaatcaat caattttcta actacaaaaa tttagaagat atcccttccg
     3361 accggcgagg gataatttct tcttacattc aagaggtaca cgatttgatg ctgtcttcct
     3421 cattggaaga tgtaaaaaaa aggtctgccg agcttaatgg cattattcat tattataatg
     3481 ataatctgca taatgctcaa aaggacattc attatttatt accgagtgat acttatattg
     3541 gcaaaagtgc ctgtgagatt ataaaatgct ttttggataa gtatattggc agtgtttgtg
     3601 tgttagagat aaaggattta cagacttacg attgtgatag atttcaatat gcgttaagtg
     3661 atgtagttag ccaaataatg attatcaaaa gaggtttact tcctgagcaa aaatttatct
     3721 ttaatttaac gggtggtttt aaatctattc tttgttttct gcaatcatta gggatgtttt
     3781 atgcggatga gacagtttat gtatttgagt attcaaagga tttaatgcgt attccagctt
     3841 tgcctgtaaa gctggtccct tatgattatc tgaaagaata catagttgtt tttcgccgtt
     3901 tatataataa tttgtctgta gcggaagagg aatatgataa aataccggaa agtttaattc
     3961 taaagctata tggtgagcca actctttcag cttatggaaa aatcgtttgg gataatttcc
     4021 gcaaagacct ctattcggaa aagctactgg attctatcag tccgaaagta aaatattctg
     4081 agattttttt gaagagtacc aaggacttag ggaaagaccg ttattataat ttaaatatgc
     4141 gtattgatga agtcagttgt tttatggagt taaaatcaaa gcccaatcct gcttctttgg
     4201 acctaaaaaa attagcaacc ccaaaaggta ttagcactca cgaatttgac gcttgggcag
     4261 ataaagatgc taaaagggtt ttttgtcatt atgaccagga tatccttgta ttagatactc
     4321 ttttagaggg tttacactaa tttcattatg tccttttcgg cttcgctgat taatttctgc
     4381 ttattttctc tggtttggaa agagctcaca aaaccgttgc ggattattgt taaaatattc
     4441 catttgctca atccgccttt agtcatttta gctgctttta aatattcttt gcttatatcg
     4501 gttcgcgaaa ttcccggatc gtcagaacat atacaggcct ttatcccttt ttgcaaatat
     4561 tgtttcaaag gataaatacg cattttggag gtagaattaa tactatagtc tcgatagctg
     4621 tttatttgaa aattgctgct ggggcaaagt tcaatagcgg ttttattttc tttaagtttt
     4681 agcatcaggt tttcatcgtc ttgcaaataa agtccgtgac caattctatc tgcattcagg
     4741 ttataaactg cttcccagat atttttcacg gaagttgttt ctccagaatg aatagtaatt
     4801 ctaatgcagt cctccagtaa aggtaatatc ttttctctta gggaaagggt tgttttctct
     4861 tcgggtcctg ccaaatcaaa accgcaaata aaatcgctga ttttcccatt ttctcttttt
     4921 atatttttac aaagatctat atgttctttt agtattctct gggttttatg tctgctgccg
     4981 attattatta agcggaaaac gcattcttca tctatcaatt cgtcatataa aatttctacc
     5041 acttcagttg cttttaactt atttgtatag ttacagggtg agcaccttaa ttctttatag
     5101 cgtaagttat tcttttttgc atcatctttc aattggtggc agatttctcg taatgcttct
     5161 tctgtttgca atagggaaga accctgaaaa tcacctattt cttcataatt ttctagtcca
     5221 atatctacaa agttgtcttc ctgcaaaagg ttgttccaga taacctgttc tagtaatttc
     5281 tctttgccct caaaacagcg taaatacaaa ctgacgagta accagcggac tttattttcc
     5341 cttgtactta tcgttttcca gattgcttta agcttattaa ggtcattctt ttggatagca
     5401 ttaatggtac ttctttccag ttttttttct tccagggtat tgcctgagta ttttaaattt
     5461 gctatagcta ttttaattaa accctcagta tcggcaaaac cgcctaaatg acagtgtaaa
     5521 tcagttttgg gtaatttata tagccagttc aaatcttgtt tgatattatc aatcccgttt
     5581 atatagttat ttttcaactc ttgaatcttt ttcggattaa gaagatgcaa aatctgaaaa
     5641 ttagaactcg gctctgaata gcgtaattta gtataataat tgattgtcag attatcctgt
     5701 tttgtctgaa gttcttccac ctggttcaga aactttgtct cgctatctga atagatatac
     5761 tcatttcctt taattttagg ttcagggaaa taaacctttt cttcctcaat aagttctgcc
     5821 aatttacttg cagggatatc cttttgataa aggacaggat taatcttatt gattacagcc
     5881 ggttctatat cttcaggtgc agctgataaa ttaaagctta catcgccttc tgctaaaata
     5941 tgaatcattg ctttacagcc gaataaataa gctgcctgtt gaatatcact actcatcgtt
     6001 tttctacctc cgctaaggca aagatagagt ttattggaat tgccttgttt atagcagttg
     6061 taaactaggc tgtatatcaa atttctcatt atcctggcat cttctgcact ggatatatct
     6121 ttcaattggt ttaaaatgtg aaattgaaga tctatattta aatgttgtgc tttaagccaa
     6181 tcttctactg cctgtttatt ggatgtgaaa ctatcatttg tgcaaatcat actaatggta
     6241 tttattggct ctaactgatt tttctctctt gtttctttca gcgtctcaaa tgacttatga
     6301 ttataataaa gaggatattg ctcataattg aaaaagccaa ccacttctgc aattaaatag
     6361 ctggtttttc ccattgagat gattaactga ctcatttact tatgcttacc tataagctta
     6421 atattttatc cattgaaaac atacacctat atttgggttc ggaagcatta aactgatagt
     6481 ggtaaacatc aattggaaca aaatgaccga tagcaattcc taaagccata caaagagcaa
     6541 tgggagctgt ctgaaacaaa tgaattcgtt gccaatgatt tttttctctt gctgcattta
     6601 aggcagaatt tatttcttgc acaaagggca gccagttttg gcttgtttcc atcactccct
     6661 gatcttgttt ggctttaatt attaagaagt tatttacctg taagttcttt tggcaaaatg
     6721 cttttgcttc tccaatcgga ttatggcttc ccagataaat aatgaatcct aattcgtttt
     6781 tttgaggatc atttatttgc agttcgcttt ggatgtactg aaaatcctct tttttaaagg
     6841 atacattttt cagttgtctg gcattttctt tcccgtataa agtaaagact tctttgtaag
     6901 tagtattgga aaattccatt tgcaatatac ttactgctct tttaaaccca aagagggcac
     6961 caatccctat ttgtaaagtg cttatttgtc cagaataaaa ccacaagacc ttcttgggca
     7021 ttatcttatc ttccaaagca ttgaatttat cttttacctg ttctttcagt aatgtttgcc
     7081 aggcattttt ttcaaaagga aggatgccat taccaaaaat agctaagtta ttagttgtta
     7141 ttccgtaaaa atcttccaag agctcatagc tgaaccagct gaagttttct tgaacttttt
     7201 gttccagtgc tttttgccag cgtataagtt cattttcttc accctgatat tgaataatgg
     7261 gtatagggat agtttct
//
LOCUS       DAORHB010000055         7136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_10270_length_7136_cov_4.721791, whole genome shotgun sequence.
ACCESSION   DAORHB010000055 DAORHB010000000
VERSION     DAORHB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10270_length_7136_cov_4.721791"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKM80_02760"
     CDS             <1..687
                     /locus_tag="PKM80_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNV92858.1"
                     /translation="GEFCESAYYLIAECYRAQKKAYSALTTLIKLQNAKLPEMDEQYF
                     YYRLGYAYELSDKLSDAVNAYRRGYELNRYSQIAYQIEDRLLELKSRNRNIDLSFLYP
                     YPKLQIAIAEETVADSLKTVQKIEPKPAAPIVATQPVSKQDLSIKLKSKPKEGFWLQA
                     GRFSIESNANRLVVNIRLLNIPATYYEDISGGKKSWVVICGSFPDRITAEEAKNLLAT
                     NNITTFLVAY"
     gene            692..3331
                     /gene="mutS"
                     /locus_tag="PKM80_02765"
     CDS             692..3331
                     /gene="mutS"
                     /locus_tag="PKM80_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNV92859.1"
                     /translation="MDKVEENKLTPMLKQYFAVKEKHPDKLVLFRMGDFYETFFEDAS
                     TASKILNITLTTRNKNDDNPVPLAGFPYHSLETYLDKLIKAGLKVVICEQTEDPKKAI
                     GLVKREVTEIITPGAVLDQSLLEGNANVFLASLYFTDFKRKIGLAHLDISTGDFLFTE
                     LDSEELVSELQRFNAAELIVDSTGKEELIKKLPMDKLPTITVFDSWQFQPQEAITTLK
                     KHFGVNTLEPYGAHNKTLGATAAGAALAYVQSLYSSPLNHITSLRYYSLSQYMQLDEI
                     SRRNLELVRSIRYGTKYGSLISVLDQTITPMGSRLLQHWLLHPLLSIEEITFRQDIIQ
                     SCIEKSSHLKELRSILKEIGDITRLVTRLGSLRINPRELISLKSYLYSAKILQNKLSS
                     FTHPQFTVWTQNMGSFEDIIALLEKSINDNPPIPITEGGIFAKGYNPELDELMDLIYD
                     GKSWIARLEEDERRKTGINNLKVGYNRVFGYYIEVSSANKNKVPDYYIPKQTLTNSER
                     FISPRLKEFEAKVLSSEEKIKNLEYELFKELRQNLAESLPRFQQLGEVIAELDVLSSL
                     AYLAWQNQYARPVFTESRELHIVEGRHPVIEKLMESDKFIPNDTDLNYPETSIAIITG
                     PNMAGKSTYLRQVGLLVILAQMGSFVPATEMTLPIFDRVFTRVGASDNLAQGQSTFLV
                     EMIETANILNSATPKSLILLDEIGRGTSTFDGLSLAWAIIEHIQKYKHSLTLFATHYH
                     ELTELENLYPDIKNYNVAVKQWNDEMIFLRKIERGGADQSYGIQVARLAGIPDRVIKR
                     AKEILKNLEEHEISPQGLTATIRKKLVRDVPQIDIFEILVDKASENDPVLNDIKEIDL
                     NDLSPVEAWQYLQKIQNKLLGEK"
     gene            3328..5025
                     /locus_tag="PKM80_02770"
     CDS             3328..5025
                     /locus_tag="PKM80_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV92860.1"
                     /translation="MNRILILTIITFLGTIFLISCAQNSTNIAGSVNGTEISLEDFYS
                     SYRGHYSIFGYQTGRTPTKEEKQKIHNETWQNITRAIILKDYYKKYNISASITEVLDT
                     LSNSIPEHIINSPRFRVNGKFDKKIYMQSLMTDKPENLMPLRKHYQEYLIPNMKLQSV
                     LIEKEMLTPKIRKQVSEIIASNADLELYIFDPSKMDISISDAEISAYYQANLEKYSLW
                     QFYRLGYCSIPVIPDSIDLSEAKNVAENIRNELSSGKEAKDVLNSVDSKNVVVSFIDN
                     GYQKTEELPLTVFNALDGLENGKCSEPVPYEKGYMIYQKIQSTKNLTLFNTIYVQSLP
                     RSVTLLAPETQAKQIVKLALDIGLEQAANEFDIEYIVTPAMSPDSLIVPANDLKGKLI
                     KSLKYAQARDILGPLYSAEQSGWYIFEVVEKQTQDYRSLDEVREEIIEELCKEHRIAQ
                     NRKLVQQWIADNSFPATYEMVKLDNINIDSLWEGKPITNIYYQAIKAYLEKAPLPQIT
                     QKDIIIVPKVISYRPGKTKPNPERIKTIYAQNLPDGWFEDWLDKQVKKARVVIYTKP"
     gene            5022..5486
                     /locus_tag="PKM80_02775"
     CDS             5022..5486
                     /locus_tag="PKM80_02775"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNV92861.1"
                     /translation="MICFSDRSHYTFMKEAIAEAKVAANEDEIPVGAVLVKNGNIILR
                     EHNRSRQLANPLAHCEKLLIDKILVSEPGFLYDYTLYVTLEPCLMCAGMIILSKIGTV
                     VYGAKDPKAGVVGSIYNVLKDKNFNHHPIVVSGILESECSSLLTDFFRKKRI"
     gene            5495..5585
                     /locus_tag="PKM80_02780"
     tRNA            5495..5585
                     /locus_tag="PKM80_02780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5529..5531,aa:Ser,seq:cga)
     gene            5605..5952
                     /locus_tag="PKM80_02785"
     CDS             5605..5952
                     /locus_tag="PKM80_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92862.1"
                     /translation="MIHKDYLIADADVPLISCDELRDELLIYNIDDSTANQLINQFEE
                     LTGKAINRFYKVNELSGGQKVILMVLLVLYSPAPKIHFKNLMQSLDTKRREAIKNLIN
                     SCGKDIILEENND"
     gene            5945..6469
                     /locus_tag="PKM80_02790"
     CDS             5945..6469
                     /locus_tag="PKM80_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92863.1"
                     /translation="MIEIPQGPIFCWQDFSLLIEKKLTFEPGKIYHLQGENGSGKSSF
                     LKHCLLPVLESKKDLIYRIYLQQLFHLQGYAIKSHSAFYKPELDLRSELDCVQYLLNN
                     LFETFSQDPRPVYCIVDENRYIQEIHRYLKENDFPFCLIYCAHSSFSLSEDVNIVNFK
                     VINPKQSIIYEATI"
     gene            6453..7115
                     /locus_tag="PKM80_02795"
     CDS             6453..7115
                     /locus_tag="PKM80_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV92864.1"
                     /translation="MKPLYRLILVALALVLFFVLFCIVYLESLTIQLAMLLIVILFSA
                     WTFSAKGLIKKIYHLLPFILLLFGVYLIFAVFQIGKSRAHWIHYGLTRTTLLLSSLLF
                     MQVLINWLRIDSFLDFPIGIDKLKYIILGKLLYRIAFSSYSELCLFVDSIPGEQQKNA
                     TLKSKFQKHLIVLLALITYVINEATLKGEMIDERIWHCYTVPNSIPVNKIELEDKIKE
                     SK"
BASE COUNT         2383 a         1218 c         1313 g         2222 t
ORIGIN      
        1 ggagaatttt gtgaatctgc ctattattta attgcagaat gctaccgtgc tcaaaagaaa
       61 gcatatagtg cccttactac acttatcaaa ttgcaaaatg ctaagcttcc tgagatggat
      121 gaacagtatt tctattaccg tcttggttat gcttacgagc tttccgataa attatctgat
      181 gccgttaatg cttaccgaag aggttacgaa ttaaatagat attctcaaat tgcctatcaa
      241 attgaggatc gtctattaga gttaaaaagt agaaatcgca atatagatct cagttttctc
      301 tatccctatc ctaaattgca gattgcgatc gcggaagaaa cagtagcaga tagcttaaaa
      361 acagttcaga aaattgaacc aaaacctgca gctccaatag tggcaactca accagtaagc
      421 aaacaggatc tttcaattaa gcttaaaagc aaacccaaag aaggtttctg gctacaggca
      481 ggaagatttt ccatagaaag caatgctaac cgtttagtag taaatatccg tctgctaaat
      541 atccctgcca cttattatga agatattagc ggagggaaaa aaagctgggt agttatttgc
      601 ggttcttttc cagatagaat aacagcagaa gaggccaaga accttctggc tacaaacaat
      661 ataactactt ttcttgtagc atattagaac agtggacaaa gtagaagaaa acaagctaac
      721 tccgatgcta aaacagtatt ttgctgttaa agaaaagcat ccagataaac tggttctttt
      781 tcggatgggt gatttttacg aaactttttt tgaggatgct tccaccgctt ccaaaatttt
      841 gaatatcact cttactacgc gtaataaaaa cgatgataat cctgttccct tagccggttt
      901 tccctatcat tctttggaaa cctatttaga taaactaatt aaagcaggtt taaaagttgt
      961 tatttgtgaa caaaccgaag accctaaaaa agcaattgga ctggtaaaac gcgaagttac
     1021 tgaaataatt actcctggtg cggttttaga tcaatctctc ctggaaggta atgccaatgt
     1081 ttttttggct tccttatatt ttactgattt taagcgtaaa ataggattag cacatttaga
     1141 tatttctact ggcgattttc tttttacaga attggatagc gaagaattag ttagtgaatt
     1201 acaaagattt aatgctgcag aattgatagt agatagcaca ggaaaagaag aactaattaa
     1261 aaaattacca atggataaat taccgactat aacagtgttt gatagctggc agtttcaacc
     1321 ccaggaagca attacaactt tgaaaaaaca tttcggagta aacactttag aaccttatgg
     1381 agcacataat aaaacattgg gagctacagc agctggtgct gccttagctt atgttcaatc
     1441 tttatattct tctccgctta atcatatcac ttctttacgg tactattctc tttcgcaata
     1501 tatgcaattg gatgaaatca gtcgtaggaa tctggaatta gttcgttcta tccgttatgg
     1561 gacaaaatat gggagtttaa tttctgtatt agaccaaact attactccta tggggtcgcg
     1621 gcttttacaa cattggctcc tgcatccttt attaagcata gaagaaatta ctttccgcca
     1681 agatataatt caatcctgta tagaaaaaag cagtcactta aaagaattgc gttcaatttt
     1741 gaaggagatt ggagatatca cccgattggt tacccgatta ggttctttaa gaataaatcc
     1801 aagagagcta atttccttga agtcatatct atattcagcc aagattttgc aaaacaaact
     1861 aagctctttt acgcatcccc aatttactgt ttggacacaa aatatgggta gttttgagga
     1921 tattatcgct ctccttgaaa aatctattaa cgataatcca cctattccaa taaccgaagg
     1981 aggtattttt gccaaaggtt ataatcctga attagatgaa cttatggatc tgatttatga
     2041 tggcaaaagt tggatagctc gtttggaaga agatgaacgt cgtaaaaccg gtatcaataa
     2101 tttaaaagtt ggttacaacc gtgttttcgg ttattatatt gaagtaagtt ctgccaataa
     2161 gaataaagtt ccagattatt atattcccaa acagacctta actaatagtg aacgttttat
     2221 ttctccgcgt cttaaggaat ttgaggcaaa agtgctttcc tccgaagaaa aaataaagaa
     2281 cttggaatat gaactgttta aagagcttcg ccaaaattta gcagaatctt tgccccgatt
     2341 tcaacagtta ggtgaagtta ttgctgaact ggatgttctt tcttcattag catatttagc
     2401 ttggcagaat caatatgcgc gaccagtttt taccgaaagt agagaattac atatagttga
     2461 aggcaggcat ccagttattg aaaagctgat ggaaagtgat aaatttattc ccaatgatac
     2521 agacctaaat tatcctgaaa ccagcatagc cataattact ggacccaata tggcaggaaa
     2581 atctacatat ttaaggcaag tcggcctttt agttatttta gcccaaatgg gaagttttgt
     2641 tcctgcgacc gaaatgacac ttcctatttt tgaccgtgtt tttaccagag ttggagcttc
     2701 cgataattta gctcagggac agagtacttt tttggtagaa atgatagaaa ccgccaatat
     2761 tcttaattct gctaccccaa aatctctgat tttattagac gaaataggta ggggtacttc
     2821 aacttttgac gggctttctt tggcttgggc aatcattgaa catatacaaa aatacaaaca
     2881 ttcactaact ctttttgcca ctcactatca tgagcttacg gaactggaaa acctctatcc
     2941 ggatattaaa aactacaatg tggcagtaaa gcaatggaat gacgaaatga tttttctgcg
     3001 caaaattgaa agaggaggcg cagatcaaag ctatggaatt caagttgctc gtttagcggg
     3061 aattcccgat agagttatta agcgtgcaaa agaaatattg aaaaacctgg aagaacacga
     3121 aataagtcct cagggcttaa cagctactat tcgtaaaaaa ttagttaggg atgtacctca
     3181 aattgatata tttgaaattt tagttgataa agctagtgaa aatgaccctg ttttaaacga
     3241 tataaaggaa attgatctga atgatctttc acctgttgaa gcctggcagt acttacaaaa
     3301 aatccagaat aaactattag gggaaaaatg aacagaattc ttattttaac aattatcact
     3361 tttttgggaa caatattcct gatttcttgt gctcaaaata gtactaatat tgccggaagc
     3421 gtaaacggca ctgaaatttc tttagaagat ttttatagtt catatcgcgg tcactattcc
     3481 atttttggat atcaaactgg acgcactcct accaaagagg aaaagcaaaa aatacataat
     3541 gaaacctggc agaacattac ccgcgccatt attttgaagg attattataa aaagtataac
     3601 atttctgcga gcattacaga agtgctggat acgctttcca actccattcc ggagcatata
     3661 attaactccc ccagattcag agttaatggt aagttcgaca aaaaaatata tatgcagtct
     3721 ttaatgacgg ataaaccaga aaatttaatg cccctccgaa agcattatca ggaatatcta
     3781 attcccaata tgaagctgca aagtgtttta atagaaaagg aaatgctaac tcccaaaatc
     3841 cgtaaacagg tttcagaaat catagccagt aatgctgatt tagaattata tatttttgat
     3901 ccttccaaaa tggatatttc tattagtgat gcagaaatta gtgcctatta tcaagctaat
     3961 ttggagaaat atagtttatg gcaattttac cgattaggtt attgttcgat tcctgttatt
     4021 cccgatagca tagacctttc cgaagcaaaa aatgtagcag aaaatatccg caacgagctt
     4081 tccagtggaa aagaagctaa ggatgtatta aattctgtag atagcaaaaa tgtggttgtt
     4141 agttttattg ataacggtta tcaaaaaaca gaagaattac ctttaactgt ctttaatgct
     4201 ctggatggct tggaaaatgg aaaatgcagt gagcctgtcc cctatgaaaa gggttatatg
     4261 atctatcaaa aaatacaaag cacaaaaaat ctaacgcttt ttaatacaat ttatgtgcaa
     4321 tcattaccaa gatcagttac tttactggct cccgaaacac aagccaagca aattgtgaaa
     4381 cttgctttgg atataggact ggaacaagca gccaatgaat ttgatataga atatattgta
     4441 acacctgcta tgagccctga ttcgttaatt gttcctgcaa atgatcttaa gggtaagcta
     4501 ataaaatctt taaaatatgc ccaagcacga gatattttgg gacctctata ttctgcagaa
     4561 caatctggat ggtatatatt tgaagttgtg gaaaaacaaa ctcaggatta tagaagtttg
     4621 gatgaagtgc gtgaagaaat tatagaagaa ctttgcaagg aacatagaat agcccaaaat
     4681 agaaagcttg ttcaacaatg gattgccgat aacagttttc ctgcaaccta cgaaatggta
     4741 aaattggata atattaatat agattccctt tgggagggca aaccaattac taatatatat
     4801 tatcaggcaa ttaaagcata tctggaaaaa gctcctcttc ctcaaataac tcaaaaagat
     4861 attatcatag tgcctaaagt aatttcttac cgaccaggaa aaaccaaacc aaacccggaa
     4921 cgcatcaaaa caatttatgc ccaaaatttg cctgatggct ggtttgagga ttggctggat
     4981 aagcaagtta aaaaagcaag agtagttatt tataccaaac catgatttgc ttttccgata
     5041 gaagccatta tacttttatg aaagaagcaa tcgcagaagc aaaagtagct gcgaatgaag
     5101 atgaaattcc tgttggtgca gtacttgtta aaaacggaaa cattattctg cgtgaacata
     5161 atcggagtag acagttagcc aatcctttag cgcattgtga aaaactcctt attgacaaaa
     5221 ttctggtttc agaacccggg ttcttatatg attatacatt atatgtaact ttagaaccct
     5281 gtttgatgtg tgccggtatg attattttaa gtaaaattgg cactgttgtc tatggtgcaa
     5341 aggaccctaa agcgggagtg gttggttcaa tatataatgt tttgaaggat aaaaacttca
     5401 accatcaccc tatagttgta agcggtattt tggaaagcga atgttccagc ttgcttacag
     5461 atttttttcg caaaaaaaga atataaaaat aaacggagag ttgccggagt ggttgaacgg
     5521 gacggtctcg aaaaccgttg tacctattaa aggtacccag ggttcgaatc cctgactctc
     5581 cgccagttgc attttggagt tttgatgatt cataaggact atttaatagc agatgcagat
     5641 gttccactta tttcttgtga tgaattacga gatgaattgc taatatacaa tatagatgat
     5701 tctactgcta atcaactgat taatcagttt gaagaactaa cggggaaagc cataaacaga
     5761 ttctataagg tgaatgaact ttcaggagga cagaaagtaa ttctgatggt gcttttagtt
     5821 ctttattcac ctgccccaaa aattcacttt aagaatttaa tgcaatccct ggataccaaa
     5881 cgtcgagaag ccattaaaaa cctgattaac agctgtggta aagatataat tctggaagaa
     5941 aataatgatt gagattcctc agggacctat tttctgttgg caggatttta gcttgctgat
     6001 tgaaaaaaaa ttgaccttcg aaccaggaaa gatataccat ttgcaaggtg aaaacggcag
     6061 tggcaaaagc tcttttttaa aacactgtct cctacctgta ttggagagta aaaaagatct
     6121 aatttaccgt atctatttac agcagctttt tcatttgcaa ggatatgcaa ttaaatccca
     6181 ttccgctttt tacaaacctg aattggactt aagaagtgag ttggactgtg ttcaatattt
     6241 attgaataat ctctttgaaa ccttttccca agatcccaga ccggtttatt gcattgtaga
     6301 tgaaaaccgt tatatacaag aaatccaccg atatctaaag gaaaatgact ttcccttttg
     6361 tcttatttac tgtgcgcatt cttcattttc tctctctgaa gatgttaaca tcgtcaattt
     6421 taaagttata aatccaaaac agagtataat ttatgaagcc actatataga ttaatcctgg
     6481 ttgccttagc gctggttttg ttcttcgtgt tattttgcat tgtttacttg gagagtttaa
     6541 ccattcaatt ggctatgtta ttaatagtta tattgtttag tgcctggact ttttctgcta
     6601 aggggcttat caagaagatc tatcatctcc taccttttat tttgctcctt tttggcgtat
     6661 atcttatttt tgccgttttt caaattggga aaagcagagc tcactggatt cattatggat
     6721 tgaccaggac tactcttttg cttagcagtc tattgtttat gcaggtttta attaactggc
     6781 tgcggataga ttctttttta gattttccta tcggtataga taaactgaag tatattatct
     6841 taggtaagct gctttataga attgcttttt catcttattc cgagctgtgt ctttttgtgg
     6901 attcaattcc aggggagcag caaaagaatg ctactttaaa aagcaaattt caaaaacatc
     6961 ttattgttct tttggcactc atcacttatg tgatcaatga ggcaactctg aaaggagaaa
     7021 tgattgatga acgaatctgg cattgttata cagttccgaa ttcaattcca gtgaataaga
     7081 ttgaacttga agataaaata aaggagtcaa aatgaaatgg tatctcattg tcggcc
//
LOCUS       DAORHB010000056         6975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_10627_length_6975_cov_4.698121, whole genome shotgun sequence.
ACCESSION   DAORHB010000056 DAORHB010000000
VERSION     DAORHB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10627_length_6975_cov_4.698121"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..691)
                     /locus_tag="PKM80_02800"
     CDS             complement(158..691)
                     /locus_tag="PKM80_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV92865.1"
                     /translation="MRWMLILFLTLAFTVLGCTPTDQPVSSEDVLNNPDKYASQEVEI
                     EVKPGEWLTDWDQAIAVAKKQKLPILVDFTGSDWCIWCKKLDSEVFSKEEFINYAKKN
                     LVLLKLDFPHGIPQSDAMKAANKKKMNEFNIEGFPTIVLVNAEGKEIARTGYRPGGAE
                     SYINHLQDLLTKQNDIK"
     gene            complement(688..1119)
                     /locus_tag="PKM80_02805"
     CDS             complement(688..1119)
                     /locus_tag="PKM80_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNV92866.1"
                     /translation="MTTIVQTHWSGEMKFTACVDGHEIVMDADPEFGGSNEGPRPKPL
                     LLAALTGCAGMDIVSLLEKMKVKDYIFSMDAQAKTSSEHPVTYQTITLFFNFQGKELP
                     PDKIRKAVNLSLERYCGVYAMLKKAANVIAKITINNQEIEL"
     gene            complement(1120..1575)
                     /locus_tag="PKM80_02810"
     CDS             complement(1120..1575)
                     /locus_tag="PKM80_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNV92867.1"
                     /translation="MADYANKFHDLITRLQMVLSEIDYAQKACLQAGRMECMLLNYLY
                     KTNGPANMNELAKVLNVSHSRVTRIMDNLVNKQLVIRRPSEEDRRCWYTQITDKGKKL
                     AANSLQTVLDQQEKILEKLPEKDVDTVYKALKQYVDKYEEVLKETLEGI"
     gene            2043..3377
                     /gene="radA"
                     /locus_tag="PKM80_02815"
     CDS             2043..3377
                     /gene="radA"
                     /locus_tag="PKM80_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNV92868.1"
                     /translation="MATIFFCTDCGYETIKWSGKCPVCGAWGTMKESTRIAGKNNKLG
                     ETITQPKPERIIDLKYDDASRLITGIKEFDLVLGGGIINGMVILIGGEPGIGKSTLML
                     QLSEWIGKQSKKVLYCSGEESAEQIRLRSKRLQVINENIYLLCTNDTEHIVEAIEENK
                     PDLVIIDSIQSVSISALDAFPGSITQLRESTNRLLRTAKTLGIPLFLIGHVTKEGVVA
                     GPKILEHMVDTVLYFEGEMRSQYKILRAVKNRFGSTNEIGLFEMTHLGLAEVNDPNQI
                     FLSHEKNDIGTAISCITEGSRSFIVEIQTLVNTANYGTPQRVAVGLDQKKMAMLLAIL
                     EKNLALYMRSSDVFINLTGGIRSTDPALDLAILAALISSMKDKPITEKTVFIGEVGLN
                     GEVRPVSQLDKRISETIKLGYNKIFISGYAKAKANPKVIKVKDIKALFSALQ"
     gene            complement(3797..5929)
                     /locus_tag="PKM80_02820"
     CDS             complement(3797..5929)
                     /locus_tag="PKM80_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNV92869.1"
                     /translation="MNRKLIAEFKYDLAPVIRGYNNRSLYINLSTNEIKQKPVTDLMI
                     DKFVGGKGFDLYLMWHGVKDDTKWDSPENEICISFGPLCGNTSYPGSGKSIVTTISPL
                     TGIPVDCNVGGHFGPYAKFSGWDAIELQGIANEEIIVYIDGDRGIVQILSAPDEEINS
                     HILAEELVKEFADDENKYQFVSVVSSGKAADNVLITCLNFSFWDKRRKVARLKQAGRG
                     GTGSVFRHKKIKAMVVKYSGLKVDSNNPVDVETIRMRGLKLHREIKAGDDTQNRMHQV
                     GTAHLMEIMNDYDLLPIRNFKYGQAPEAEGLHSREFKQLFTQGMPDGCWFGCSLSCCK
                     GVDNFEPRTGPYKGQKVCVDGPEYETAAGCGSNIGVFNARDVVEINFYCDTYGVDTIS
                     FGTSTAFAMECYENGILNKERTGGLELTWGNADAALELLHQMARGEGFGLIVGQGVRK
                     MKQIFAEKYGADPNFLQDIGMEAKGLEYSQYLSKESLAQQGGYTLALKGPQHDEAWLI
                     FMDMVNNQIPTFKDKAEALHYFPMFRTWFGLQGLCKLPWNDIEPADNAETDEPAKVPE
                     HVQNYVDIYKAITGKPLDKKTLIEQSERVYNFQKVFCLRMGKGRRIDDVPPYRAVGPV
                     TEEEYLSRQERYDKQLKEKIGINPEGKSTQEKMEILRNYREDQYQQLIDAVYDRKGWT
                     KEGVPKIEHLEKIGMDLPEVIEVVKRFI"
     gene            complement(5946..6329)
                     /locus_tag="PKM80_02825"
     CDS             complement(5946..6329)
                     /locus_tag="PKM80_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV92870.1"
                     /translation="MKVLRTYPDKCITCHNCEAACSKLYFKEDNPEKSCIVINEELYP
                     PEMTVCNQCGICAGVCPTLALTINAQEVVILNKSLCIGCLMCVAACPNNSMRYAKGVY
                     NPFKCIACGICTKSCPAEAIEIETA"
     gene            complement(6394..>6975)
                     /locus_tag="PKM80_02830"
     CDS             complement(6394..>6975)
                     /locus_tag="PKM80_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92871.1"
                     /translation="ILLRKRVKKLSAYLYASSLQNFYLEPCFLNGHSPYKSPLLLANR
                     TAQSLLATFVMEDWDKDNGDLQNINFNAVLSFNFGKYLGLDGQYIYIVNKDNIVEETS
                     KGAGVIADMRLSFISYNIFRIIGNLEYSYYDIDYLDVSENLNYGFYGILQLTNHLNGT
                     IFYKINNVWNRAEDLYETDHSYDILGFTASIRL"
BASE COUNT         2144 a         1396 c         1157 g         2278 t
ORIGIN      
        1 aaaccctgct tctatataga atgcagatat aatacactat gttattttgg gtaaacctaa
       61 gatcatatcc tatgcgttgg tttttaaact agcggtgctt tacaaagtgg aaagtgataa
      121 tgatctttgg caaactgagg caatattcag ttttccctca tttgatatca ttttgtttag
      181 ttaacaaatc ttgcaaatga ttgatatagc tttctgctcc cccgggtcta taacctgttc
      241 tggcaatttc cttcccttct gcattaacca aaacaattgt gggaaagcct tcaatattaa
      301 attcattcat tttctttttg tttgcagctt tcattgcatc gctttgtggt atcccatgag
      361 gaaaatccaa ttttaataat accaaattct ttttggcata atttataaat tcttctttac
      421 taaagacctc gctatccagt ttcttgcacc agatacacca atccgaaccg gtaaaatcaa
      481 ctaaaatcgg aagcttttgt ttttttgcaa ctgctattgc ttggtcccaa tcggttagcc
      541 attctccggg ttttacttca atttctactt cctgagaagc atatttatca ggattattca
      601 aaacatcttc gctgctaaca ggttgatcag ttggggtaca acccaaaaca gtaaaagcta
      661 gcgttagaaa caaaattagc atccatctca taattcaatc tcctgattgt tgatggttat
      721 tttggctatt acatttgcag cttttttcag cattgcatat acgccacaat atctttcgag
      781 ggataaatta accgctttcc ttattttatc gggcggaagc tctttaccct gaaaattaaa
      841 aaaaagtgta attgtttgat atgtaacggg atgctcagac gatgtttttg cctgagcatc
      901 cattgaaaaa atatagtcct taaccttcat tttctccaat agggaaacaa tatccattcc
      961 tgcacaacca gtaagagctg cgagtaataa aggtttcggc ctgggccctt cattacttcc
     1021 tccgaattca ggatcggcat ccataactat ctcgtgtcca tcaacacaag cggtaaattt
     1081 catttcccca gaccaatggg tttggacaat tgtagtcatt tatatccctt cgagagtttc
     1141 tttcaaaacc tcttcatact tatccacata ctgttttaac gctttataaa cagtatcaac
     1201 atctttttcg ggaagttttt ccaggatttt ttcctgctga tctaaaactg tctgtaaact
     1261 attagcagcc aattttttac ccttatcagt aatttgggta taccagcaac gacgatcttc
     1321 ttctgaaggt ctacgaataa ccaattgttt atttacaaga ttatccataa ttctggtaac
     1381 ccgactatgg gatacattaa gcacttttgc cagttcattc atatttgcag gtccattggt
     1441 tttgtataga tagttcaaga gcatacattc cattcgccct gcttgtagac atgctttttg
     1501 agcatagtca atttcgctta acaccatttg taagcgagta attagatcgt ggaatttgtt
     1561 agcataatca gccatttgat tctccttttt agtgtttgtt tgatctcact gttttttttc
     1621 gatccatctc cagatcgaaa ttaaccgttg gaaagtaatt tatccaagtt ttatgggttg
     1681 ctaatgcttc ccttttgcgg atcataaatt tgcgagccgc aagaccatta actacatcta
     1741 tcctagtaaa tatgttaatt aaaaccatcc ggaaactccc ttcctgtttt acttaaaata
     1801 taaataaaac tactgcctac cttcacgttc gcattcattt ttctatcctc tgttcatata
     1861 ataacgcaat ttttctaaag agcaaaaact tttttcattt tttgaaaatt ttctcttgtg
     1921 tcagtgattg atattttaat tgcggaattg ctgttaatga tatttaacaa aggtaagcag
     1981 taaatttgct tgccataaaa tacacatcag attattagga aataaatgta aaggagaact
     2041 aaatggctac tatatttttc tgtacagatt gtggttacga aactatcaag tggagcggga
     2101 aatgtcctgt ctgtggagct tggggaacaa tgaaagaatc cactcgtatt gcaggaaaaa
     2161 ataataagct cggagaaaca atcactcagc ctaaaccgga aagaattata gacttgaaat
     2221 atgatgatgc ttctcgttta ataaccggca taaaagaatt cgatttagtt ctcggtggtg
     2281 gaattattaa tggaatggtg attttaattg ggggtgaacc aggaataggt aaatccactt
     2341 taatgttaca actttcggaa tggattggaa aacaaagcaa aaaagtgctc tattgctcag
     2401 gagaagaaag tgcagaacaa attcggttgc gtagtaaacg actgcaagtt ataaatgaaa
     2461 atatttattt actctgcacc aacgatacag aacatattgt ggaagcaata gaagaaaata
     2521 agcctgatct ggtaattata gattccattc aatcggtaag tatttctgct cttgacgctt
     2581 ttccaggtag tattactcag cttagagaaa gcacaaatcg tttattaaga accgctaaga
     2641 ccttaggcat cccccttttc ctaattgggc atgtaacaaa agaaggtgtg gttgcaggac
     2701 ctaagattct ggaacatatg gtagataccg tactctattt tgaaggagaa atgcgtagcc
     2761 aatataaaat tctacgagca gttaaaaacc gttttggatc tacaaatgaa attggcctat
     2821 ttgaaatgac tcaccttggg ttagctgaag ttaacgaccc caaccagatc tttcttagcc
     2881 acgagaaaaa tgatatcgga actgccataa gttgcatcac agaaggaagc cgtagtttta
     2941 ttgtcgaaat ccaaaccctg gttaatactg ctaattatgg aacccctcaa agagttgcag
     3001 tggggttgga tcagaaaaaa atggctatgc tgctggctat tttggaaaaa aatcttgctc
     3061 tctatatgcg gagtagcgat gtcttcataa atttgacagg aggaattaga agcacagatc
     3121 ctgcactcga tttagctatt ttggctgctc tgatctccag tatgaaagat aaacccatta
     3181 cagaaaaaac tgtgtttatt ggtgaagttg gactaaatgg agaagttcgt cctgtttctc
     3241 aacttgataa acgaataagc gaaaccatca aacttggcta taacaaaata ttcatttccg
     3301 gttatgcaaa agcgaaagct aatccaaaag ttataaaagt aaaagatatc aaggcactat
     3361 tttctgcatt acagtaaaca atcacggtga tgttatctct cgcagatttc gctgattctg
     3421 tagattttat ctatattgta tgttgaaagt acgaggaagt gtctcactac tataaagctc
     3481 ctttgaaacg attttggata tacaataatg tatcttcagt gaaggctcgc ttcatagatt
     3541 gacatatgaa ctgttttaca actgtatttt tcgttttggg acagccccga cagatattaa
     3601 taatgggttt taacccagcg ttaaaatcta tcgttactag cggaactctt atatgaaatc
     3661 ctcttaatct aaaaacttat gtttctaaaa ttcaatcctt aaactcggtg agcagcataa
     3721 aaaaaccctc actggcacaa aggagtttct aaaccataga aaagacagca gtgagggtat
     3781 agcaatgtat attattttaa ataaagcgtt taactacttc aataacttct ggcagatcca
     3841 ttccgatttt ttctaaatgt tcaattttgg gaacaccttc tttggtccag ccctttcgat
     3901 cgtaaaccgc atcaataagt tgctgatatt gatcttcgcg gtaatttcgc aatatctcca
     3961 ttttttcttg ggtgcttttt ccttctgggt tgattccaat tttctccttc agttgtttat
     4021 cgtaacgttc ttgacgggaa agatattctt cttcagtaac agggccaacc gctctatagg
     4081 gtggcacatc atcaattctt cttcccttgc ccattcgcaa acaaaagacc ttctggaaat
     4141 tatagacgcg ttctgattgt tcaataaggg tctttttatc cagtggttta ccggtaatgg
     4201 ctttatagat atccacataa ttttgaacat gttccggaac ttttgctggt tcatcggttt
     4261 cagcattatc tgcgggttca atatcgttcc aaggaagctt acataatcct tgtaaaccaa
     4321 accaggttct aaacattgga aaataatgca atgcctcagc tttgtctttg aaggtaggaa
     4381 tctggttatt taccatatcc ataaaaatta gccaggcttc atcatgttga ggtcctttta
     4441 aagcaagagt ataaccacct tgttgagcaa gtgattcttt actaagatat tgtgaatatt
     4501 ccaagccctt ggcttccatt ccgatatcct gtaaaaagtt tggatcggct ccatactttt
     4561 ccgcgaaaat ttgtttcatt ttccgcacac cctgtccaac aattaatcca aaaccttctc
     4621 ctcttgccat ttgatgcaaa agttctaaag ccgcatctgc atttccccaa gttaattcaa
     4681 gtccgccagt gcgttcttta ttcaaaatgc cattttcata acattccatt gcaaaggcag
     4741 tgctggttcc gaaagaaata gtatccactc cataggtatc gcagtaaaaa ttaatttcta
     4801 ctacatcccg ggcattaaaa acgccaatat tacttccaca accggcagct gtttcatatt
     4861 caggaccatc cacacatact ttttgccctt tatatggtcc ggttcttggc tcaaaattat
     4921 ctaccccttt acaacaagat agagaacagc caaaccaaca tccatcgggc ataccttgag
     4981 tgaaaagttg tttaaattcc ctggagtgta aaccttccgc ttcaggggct tgtccatatt
     5041 taaaattccg gatcggtaac aggtcataat cattcataat ttccataaga tgggcagttc
     5101 ctacttggtg cattctattt tgagtatcgt ctcccgcttt aatttcacgg tgtaatttta
     5161 aacctctcat tctgatggtt tctacatcga cagggttatt tgaatccact ttcaaaccgg
     5221 aatattttac aaccatcgct tttatttttt tatgtctgaa cacactaccc gtaccacctc
     5281 taccggcttg ttttaagcga gcaacttttc tccgtttatc ccaaaaggag aaatttagac
     5341 aggtaattaa aacattatcg gcagctttcc cgctggaaac tacagataca aattgatatt
     5401 tattttcatc atctgcaaat tcttttacca gttcttccgc taaaatatga ctatttatct
     5461 cttcatcggg agcagaaaga atttgcacaa ttcctctgtc gccatcgata tagacaataa
     5521 tttcttcgtt ggctatgcct tgaagttcaa ttgcatccca accagaaaat ttagcataag
     5581 gaccaaaatg accacctaca ttacagtcaa ccggaattcc cgttaagggt gaaatagtgg
     5641 ttacaataga ttttccgcta cctggataac ttgtatttcc acaaagcgga ccgaaagaaa
     5701 tacagatttc gttttcgggg ctatcccatt tggtatcatc tttaacgcca tgccacataa
     5761 gataaagatc aaaccccttt cctcctacaa atttatcaat catcagatca gttacgggtt
     5821 tctgttttat ctcattagtg ctcaaattta tatacaacga gcggttatta taaccccgaa
     5881 ttacaggtgc caggtcatat ttgaattcag caattaactt acggttcatt tttctactcc
     5941 tcttcttaag cagtttcaat ttctattgct tctgcggggc aagattttgt acatatgcca
     6001 caagcaatac atttaaaggg attataaaca cctttagcat aacgcattga attatttgga
     6061 caagcagcta cgcacatcaa acagccaata caaagagatt tatttaaaat aacaacttcc
     6121 tgagcattaa ttgttaatgc caaagtggga caaacacctg cacatatacc acattgattg
     6181 caaactgtca tttctggagg ataaagctct tcattaataa ctatgcacga tttttcagga
     6241 ttatcctctt taaagtatag cttggaacaa gcagcttcgc aattatgaca ggttatgcat
     6301 ttatctggat aggttcttaa cactttcata tcaaactcat ccccctttat tgttacttag
     6361 attcttttta ataaaaacgg tgttttccgt caactacaat ctgattgaag cagtaaaacc
     6421 taagatatca taggaatgat ctgtctcata tagatcttca gctctattcc atacattatt
     6481 aatcttataa aaaatagtcc cgtttaaatg attggtcagc tgcaaaatac cataaaatcc
     6541 ataatttaaa ttttcagata catctaaata atcgatatca taatacgaat attccaaatt
     6601 accaataatc cgaaatatgt tataactgat aaaagataat cgcatatcag caatcacacc
     6661 tgcgccttta ctcgtttcct caacaatatt gtctttattt acaatatata tatattgacc
     6721 atcaagtccc aagtattttc cgaaattaaa agaaaggaca gcattaaaat tgatattctg
     6781 gagatcacca ttgtccttat cccaatcctc cattacaaag gtcgctaata aactttgggc
     6841 tgtccgatta gctaatagga gggggctttt atagggagaa tgcccattca aaaaacaagg
     6901 ttcgagatag aaattttgca agctggaagc ataaagataa gcacttaact tttttactcg
     6961 tttcctcaac aatat
//
LOCUS       DAORHB010000057         6915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_10772_length_6915_cov_4.257872, whole genome shotgun sequence.
ACCESSION   DAORHB010000057 DAORHB010000000
VERSION     DAORHB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10772_length_6915_cov_4.257872"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3841)
                     /locus_tag="PKM80_02835"
     CDS             complement(<1..3841)
                     /locus_tag="PKM80_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV92872.1"
                     /translation="METLKSKALAENLAVTKVSEIVLDDQAKWLLSLTEGNYGIKQRC
                     RNFLEELYHPYTNPEVVATLIRQSILGDLWFYVQLPESKQALTVIGDLYLLAAKKCSK
                     PLQEKRIIVEYLDYISALCRTEGIPKDVYSYLLDELNNWQNRSKELFIPINGTFRKCL
                     SNLVQVYECNEICFVIMKDLLLEGLEFWQRTSDIEGWGEKTSSENGFDIDYLSANLGK
                     KYYEKWLKELEDRKDFQSLQDIPSFTDIAILHRETICEFKTLTLRIKYIFYLLGLPGM
                     EELRDHLLWDLNRQLADLETELNEDEVPVMLDSVFKTLYEFTDTHLSIVLDCILTIGK
                     TVLSHKNKKHCQHLLPKIMELEFTPPGKIKIDKDWQIEVDKNHIKHLRMLLELIAIDP
                     LMCKDLLAYLIISLSQKGVFISDTDLFQKDVSAFLNSDFKPILVQTKHLLKMFPVFFN
                     EIGAEGEIRDISTSIDELGHRKDLLMHFLRKQIHTESNNTHLLLIQKILNYWTTLDYR
                     ELQDMIPEDVLQYVKNLDEMTREQSAAVNRFLKKHNLIPEELLSLSWQRVNKLFEGEP
                     EDFSLKRLKLLCYSHFLLKDKYNLDPYDIVKFLSRYSWFDAHEQNRLANSFMRKDYDS
                     SIRQLLSYIGKMNSVILDPHTTTAWENIYYKRHIAAGIPSMYGMYREPKLEAMGMIFR
                     IENVVRRLFEHNVQQLNLNYITGKTLCRIIRILELYDYAMKQIMVTNDAYSSALDMLR
                     SAQYLSNLSIDQYLDIFKLLKDSVVEIINEYYYRFYDPQLRNIGLGIAEKAKDKRKQG
                     ITETEIFAEEFYRNLLSNSFLVQRLDNFITRILESLSEMKHLFKPADIPKVMSYDPDK
                     LFFHLNKKNSRIENQVLLGSKAYFLKRMHQYEFPVPPGFVITTDLYRHRNIINTHPDI
                     SNELDSLIMHNLNILEKNTGFVLGDPEKPLLLSVRSGAPMSLPGAMNTFLNIGLNDEV
                     TYKLSRRPNYGWTAWDCYRRLIQSWGMAYGILRDEFDAIMIKYKKRYDVELKTQFSSA
                     QMQEMVQDYKNVLFEHNLELEQNPFMQLYKAISNVLDSWNADRARLFRQKLHIADEWG
                     TAVIIQQMILGNISLESGTGVLFTHYDFSKEGGICLNGDFTLCSQGEDVVAGLVHTLP
                     ISETQRLHSQTPVADSLENTFPAIYNELLRYAKQLIEDKNYPHQEMEFTFEGPSPDQL
                     YILQTRNQVIQKAPDYKVFGSTKGDMKKLAYGIGIGKGTINGIVVFNQEDIKKYADKG
                     EALILVRPDTVPDDMELL"
     gene            3991..5196
                     /gene="dnaN"
                     /locus_tag="PKM80_02840"
     CDS             3991..5196
                     /gene="dnaN"
                     /locus_tag="PKM80_02840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNV92873.1"
                     /translation="MEKIIINQSEVMMRLAIEKKDLLAHIQHLVSIVPSKNVTPILNN
                     YLIEVSEETNMMRISTTDLTLTVVVEFPAAVSEGGTIAVSAHHFNEIINYMPDAVINL
                     WQHDDLLMIQSGKVDFNLLIADHTLFPVIPEPKINYPITVDAALFNRMISKTYFAVST
                     DVNRAILTGVCWKIYPTYHMMVATDGRKVAEIKVLNSSLLPSLENNDNQGLSIFTEQN
                     EVYVEKVIPLKTLLFLQKIFSEDVKELKVSMEHNRIMFLYGEYFVSSQVFEHKYPEYQ
                     KAFPTELPNKFVIDKESLITAIKRVALIAPDENFRIHFDIDNDRFEVNTTNRDTGDAK
                     QNMDDYVYAGTSTGISFNYKYMLSILEAIDTEKVVIKLGSSKDPMMIYNENPIPDQEI
                     TFLLMPLRS"
     gene            5202..6275
                     /gene="recF"
                     /locus_tag="PKM80_02845"
     CDS             5202..6275
                     /gene="recF"
                     /locus_tag="PKM80_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198307.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNV92874.1"
                     /translation="MNLVNIELENFRNYRNSTYSVGPLGSLIIGPNGCGKTNLLEAIA
                     YCSIGKSIHFHHDEELLHFGERFFRVQGSFISDVGTPIKVALGYAEQRKLLKIDDLPI
                     RQLSTLFEIVKIIYCAPEDHLLITGSPRFRRQYFDLAIAQLYPPYITALRHFLHMVQQ
                     RNAMLKKNYTKEEQISWNTSFATSLAEVWNYRNRYLTHINTAFKEIFKDVFPTSASIT
                     ISYLPSLKMPLDSSIDDIVQYLSHLAEREKLLQRSLVGAHLDDYEFRLEGKKMRSYAS
                     QGQKRIAVIILKLIQAHLIEKVTGIKPILLFDDIFAELDAFHSEAIRNCINNHYQVFI
                     ASPKEDICLIWQGYPILRLGEEH"
     gene            6276..>6915
                     /locus_tag="PKM80_02850"
     CDS             6276..>6915
                     /locus_tag="PKM80_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV92875.1"
                     /translation="MKLNRNILGWMFYDFANSAFTTIIVSVVYSVYFIKSVVGGPSGY
                     GEMLWGRAIGLSMTLVAISAPILGAVADYSRAKKRMLFINCYLTVIFTALLYFVKPGD
                     VFLGMLFFIIANFGFNSANVFYDAFLPEICQPEDIGKVSGFGWSLGYVGGLVSLLVAL
                     VLVKINVRLVFPAVALHFFLFSLFTFFWLKEFRRPSQRSNYYRIAWQRVIYSL"
BASE COUNT         2131 a         1273 c         1202 g         2309 t
ORIGIN      
        1 acaaaagctc catatcatct ggaacagtat caggacgcac taaaattaaa gcttcccctt
       61 tatctgcata ttttttaatg tcctcttgat tgaaaacaac aatgccatta attgtccctt
      121 tcccaatccc tataccataa gcaagtttct tcatatcccc tttagttgag ccaaaaacct
      181 tatagtcagg tgctttttga attacttgat tgcgggtttg caaaatatat agttgatcag
      241 gagaaggtcc ttcgaaggta aattccattt cctgatgggg gtaattttta tcttcaatta
      301 gctgcttagc atagcgtaat agttcattat aaattgctgg aaaagtgttt tctagagaat
      361 cagcaaccgg tgtttggcta tgcaaacgct gagtttcgga aataggtaaa gtgtgcacca
      421 aaccagctac aacatcttct ccttgactgc aaagagtaaa atcaccattt aaacaaatac
      481 ctccttcttt actaaagtca taatgagtaa agagaactcc cgttcctgac tcaagagaga
      541 tatttcccaa aatcatttgt tggataatta ctgcagttcc ccattcatcg gcaatatgca
      601 atttttgacg gaataatcgt gctctatctg cattccagct atctaaaaca ttagaaattg
      661 ccttgtagag ttgcataaaa ggattttgtt ccagctcaag attatgttcg aaaagaacat
      721 ttttatagtc ctgcaccatt tcctgcattt gagcactgga aaactgtgtt tttaattcaa
      781 catcataccg ttttttgtat ttaatcatta ttgcatcgaa ttcatcacga agaattccat
      841 aagccatacc ccagctttgg attaagcgtc tatagcaatc ccaagcggtc catccataat
      901 ttggacgccg ggatagttta taggtaactt catcgttgag gccaatattt aaaaaagtat
      961 tcatagctcc aggtaaactc attggagctc ctgaacgtac agaaagtaat agtggttttt
     1021 ctggatcgcc caaaacaaag ccagtattct tttccagaat attcaaattg tgcataatca
     1081 aggaatccag ctcattggaa atatcaggat gcgtgtttat aatattccgg tgtcgataaa
     1141 ggtctgtagt tataacaaaa cctggaggaa caggaaattc atattggtgc attcgtttca
     1201 gaaaatatgc tttggaacct aataacactt ggttttcgat ccgggaattc tttttgttca
     1261 agtggaaaaa tagcttgtcc ggatcataac tcattacttt aggtatatcg gctggtttaa
     1321 ataaatgttt catttcggaa agagattcca agattcgtgt aataaaatta tccaacctct
     1381 gcactagaaa tgagttagac agtagattac gatagaattc ttctgcaaag atttccgttt
     1441 ctgtaatccc ctgttttctc ttatccttag ccttctcagc tatccctagg ccaatattcc
     1501 ttaattgcgg atcataaaac ctgtaataat attcgttaat aatctccact acactatctt
     1561 taagcagctt gaaaatatcc agatattgat ctatagaaag gttgcttaaa tactgagcag
     1621 atcttaacat atccaaagca gaactatatg catcgttagt aaccataatt tgtttcatcg
     1681 cataatcgta aagttccagg atacgaatta ttctacacaa ggttttaccg gttatgtagt
     1741 ttagatttaa ttgttgtaca ttatgttcga agaggcgtct tactacattt tcgatcctaa
     1801 agatcatacc cattgcttcc aatttgggtt cacgatacat tccatacata gaaggtatgc
     1861 cagcagcaat gtgtcgctta taatagatat tttcccaggc agtagttgtg tgaggatcta
     1921 atataacgct attcatcttt ccaatatacg atagcaattg cctgatagaa ctatcataat
     1981 cttttctcat aaaagaatta gcaaggcgat tttgctcgtg agcatcgaac caactgtatc
     2041 gagacagaaa tttaacaatg tcataaggat caagattata tttatccttc agcaaaaaat
     2101 gagaatagca aagcagttta aggcgtttta atgaaaaatc ttctggttca ccttcgaata
     2161 atttgttaac cctttgccag gatagagaga gcagctcttc aggtataagg ttatgtttct
     2221 ttaaaaatct gttaactgca gcactttgct ctctagtcat ctcatcgaga ttcttcacat
     2281 actgtaaaac atcttccgga atcatgtcct gcagttctct ataatccaaa gttgtccaat
     2341 aattcagtat tttctggatt aataaaaggt gagtgttatt gctttcagta tgtatctgtt
     2401 tcctcagaaa gtgcattagt agatcttttc tatgccctaa ttcatctata ctggtgctga
     2461 tatctcttat ttcaccttca gctccaattt cattgaaaaa aacaggaaac attttcagca
     2521 ggtgtttagt ctgcactaat ataggtttaa aatccgaatt taaaaatgca gaaacatctt
     2581 tctggaaaag atcagtatcg gatataaaaa cccctttttg gcttaaactt ataatcaaat
     2641 aagcaagaag atctttacac atcaatggat caatagcaat cagctccagc agcatacgca
     2701 agtgcttgat atgattttta tcaacctcaa tctgccaatc tttatctatc ttaatcttcc
     2761 caggaggcgt aaattctagt tccataatct taggtagcag atgctgacag tgttttttat
     2821 tcttatggga aagcacagtt ttgccaatgg ttaaaataca atccaataca atagataggt
     2881 gagtatcagt aaattcatat aaggtcttaa aaactgaatc cagcattaca ggtacttcat
     2941 cttcattcag ttctgtttcc aggtcagcta attgacgatt taaatcccag agcagatgat
     3001 cgcgcagttc ttccatacct ggtaaaccca aaaggtaaaa aatgtacttt attctgagag
     3061 ttagagtttt aaattcacag atagtttcac gatgaagaat agcaatatct gtgaaggaag
     3121 gaatatcttg taaagattgg aaatcctttc tatcttctag ttctttcaac catttctcgt
     3181 aatatttttt acctaaatta gcagaaagat aatctatatc aaacccattt tcagaacttg
     3241 tcttttcgcc ccaaccttca atatcagatg ttctttgcca aaattccaaa ccctctagta
     3301 aaaggtcttt cattataacg aagcatatct cattacactc atacacttgc acaagattgg
     3361 ataaacactt gcgaaaagtt ccatttatcg gaatgaacaa ttctttgctc ctattttgcc
     3421 agttattaag ttcatccaat aaatatgagt atacatcttt gggtattcct tctgtgcggc
     3481 aaagcgcaga tatataatcc aggtattcaa ctattattct cttttcctgc aaaggtttac
     3541 tgcatttttt agcagcaaga agatataaat caccaataac ggttagggct tgtttacttt
     3601 ctggcaattg aacataaaac cataaatctc ctaagatact ttgcctgata agggttgcca
     3661 ccacttcagg atttgtataa ggatgataca gttcttccaa aaaattccgg catcgttgct
     3721 taataccata attaccttct gttaaagaaa gtaaccattt agcttggtca tcgagcacaa
     3781 tttcggatac tttggtaact gcaagatttt ctgctaatgc ctttgatttc agggtttcca
     3841 agacctaaac ctcttaattt tattgtataa caaaacagct atatattctt tttatatttc
     3901 cgctggattc tgtcaaggag tttttgactt ttattttttg gctaaccgaa acataaaaaa
     3961 gtgattgact aaataacata gacatttttt atggaaaaaa taataataaa tcagagtgag
     4021 gttatgatga gattggccat tgagaaaaaa gacctacttg ctcacattca acatcttgtg
     4081 tctattgtcc cttccaaaaa tgtaactcct atactgaata actatttgat agaagtttcg
     4141 gaagaaacca atatgatgcg tatcagtaca acagatttaa cgcttacagt tgtagtagaa
     4201 tttcctgctg ctgtttccga aggtggtact attgctgttt cggcacatca tttcaatgaa
     4261 attattaatt atatgcccga tgcagttatt aatctttggc agcatgatga cctgttaatg
     4321 attcaaagcg ggaaagtgga ttttaatcta ctgattgctg atcacactct atttcctgtt
     4381 attcccgaac ccaagataaa ttaccctatc actgttgatg ctgcattatt taaccgaatg
     4441 atttccaaaa cttattttgc agtatcgacc gatgtaaatc gggctatttt aacaggtgtc
     4501 tgttggaaaa tttatccaac ctatcatatg atggtagcaa ctgacggacg taaagtagct
     4561 gaaataaaag tattgaattc ttccttactt ccttctctgg agaataatga taatcagggt
     4621 ttgagcattt ttacagaaca gaatgaagta tatgtagaaa aagttattcc attaaaaacc
     4681 ctgctgtttt tacaaaagat tttcagtgaa gatgtgaaag aactgaaagt atctatggaa
     4741 cataatcgga ttatgttttt atatggagag tactttgttt cctcgcaagt ttttgaacat
     4801 aaatatcctg aatatcaaaa ggcatttcca acagaactcc ctaacaaatt tgttattgat
     4861 aaggaatctc tgataactgc catcaagcga gttgccctta ttgccccaga tgagaatttt
     4921 cgcatacatt ttgatataga taacgatcgc tttgaggtaa atacaacgaa tagagatact
     4981 ggtgatgcaa agcagaatat ggatgattat gtttacgcag gaacctctac aggaatttcc
     5041 tttaactata aatatatgct ttctatttta gaggcaatcg atacagaaaa ggttgttatc
     5101 aaacttggct cctcaaaaga tccgatgatg atttacaacg aaaatcccat tcctgatcag
     5161 gaaattacct ttctgctaat gccattacgt tcataaaaaa tttgaacctg gttaatatcg
     5221 aacttgagaa tttccgcaac tatcgtaaca gtacatatag tgttggcccc ttaggatcat
     5281 taattattgg tcccaatggt tgtggtaaaa ctaatctcct ggaagcgatt gcttattgca
     5341 gtataggtaa atccatccat tttcatcatg atgaggaatt gctgcatttt ggagagcgtt
     5401 tttttcgagt tcaaggttca tttattagcg atgtgggaac cccgataaag gttgctttag
     5461 gttatgctga acagcggaaa ctattaaaga ttgacgattt acccattcgt cagctgagta
     5521 cgcttttcga aattgtgaag attatttatt gtgctccgga agatcatctt ttaataactg
     5581 gttctcctcg ctttcgcaga cagtattttg atctggcaat agctcagctt tatccgccct
     5641 atataactgc tttaaggcat tttttacata tggttcaaca acgaaatgct atgctgaaaa
     5701 aaaattatac aaaagaggaa caaattagtt ggaatacatc ttttgccact tctttagccg
     5761 aagtttggaa ttatcgtaac cgttatctga cacacattaa tactgccttc aaagaaatct
     5821 tcaaagatgt cttccccact tccgcttcca tcactatatc ctatcttcct tccttaaaaa
     5881 tgcctctgga cagttctatt gacgacattg tacagtatct cagccatttg gcagagagag
     5941 agaaattgtt gcaacgctcc ctagttggag ctcatttaga tgattatgaa ttcagattgg
     6001 aaggaaaaaa aatgcgaagc tatgcttctc aaggtcaaaa acggatagca gtcattattc
     6061 taaaacttat acaagcacat ttaattgaga aagttacagg tataaagcca atattgctat
     6121 ttgacgatat ttttgccgaa ctggatgcct ttcattcaga ggcaatccga aactgtatca
     6181 ataatcatta tcaggttttt attgcaagtc ccaaagaaga tatttgccta atatggcaag
     6241 gttatccaat tctgcgtctg ggagaagagc actaaatgaa actgaaccgt aatattctgg
     6301 gttggatgtt ttatgatttt gccaattctg cgtttacaac gatcattgta tccgtagtgt
     6361 acagtgtata tttcataaaa agcgttgttg ggggtccttc tggttatgga gaaatgcttt
     6421 ggggaagggc aattggatta tcaatgacgc tggtagctat ttcagctcct attttaggtg
     6481 cagttgcaga ttattcgcgt gcgaaaaaga gaatgctatt tatcaattgc tacctaactg
     6541 tgatttttac agccttgctt tattttgtaa aaccgggtga tgtgtttctt ggtatgcttt
     6601 ttttcataat tgctaacttc ggtttcaata gtgctaatgt gttttacgat gcttttttac
     6661 cagaaatatg ccaacctgaa gacataggca aggtttcagg ttttggttgg tctttaggtt
     6721 atgtaggggg tttggtttcc ttgctggtag ctttagtttt ggtaaaaatc aatgtgcgat
     6781 tggtttttcc tgctgtagca ttgcattttt tcctgttttc gctattcact tttttctggc
     6841 tgaaagaatt ccgccgtccc tctcaaagaa gtaactatta tcgtattgcc tggcagagag
     6901 ttatttattc cttaa
//
LOCUS       DAORHB010000058         6853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_10936_length_6853_cov_4.439394, whole genome shotgun sequence.
ACCESSION   DAORHB010000058 DAORHB010000000
VERSION     DAORHB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10936_length_6853_cov_4.439394"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKM80_02855"
     CDS             <1..368
                     /locus_tag="PKM80_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HNV92876.1"
                     /translation="LVDICQAILQSEINKIELKFSDKTALGVVLASGGYPLSYQKGFP
                     LIMPSTLSGIYFSGVADAENGLVTSGGRVLCAVGVGKDLNEAREEAYSKAQQITFENR
                     IFRQDIGLRNNTLLDNGDK"
     gene            373..5454
                     /locus_tag="PKM80_02860"
     CDS             373..5454
                     /locus_tag="PKM80_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNV92877.1"
                     /translation="MTIYSIIQVKNLSLAKFYKAGLLFYLSFFLPALIFASPITLISE
                     SQDLLTIQFELPEYELEDVTINGQTWKRIICSESSIYGEEGYPELKLFSTAIAVPVDG
                     DISFSIESSESQTLKNVNIYPAATMLLEESEPIFVNKPNYKAYSNRELYPLQVVQKGE
                     PAFVGNRRFVPLFICPFQYRAQSKELIIHRSITVSVYVAGSKNASKNWQLNPNPLDNA
                     TPSLFLNENSSKIWRLEKTRDTNYTSPKNGTSVINEIQIIVDKEGIYKVDYHYLMDYI
                     NMMVDSLQVNMNWTPANVDPRYLELSDEYGQVPINFVGENDGIFNPNDYFEFYGDMHK
                     GDTSYMDDYTSENVYTLKLVDRFGARMAVENGGLIESNISNCIVPDAYEDTVHFEEQL
                     ISDKLGQGWSSIDQNYYREDVWFWRKIDAPDLEIVPIELQYPKDTTTRYASAKICLMG
                     LTYSETLTSGQFDHEASIRLNQAMINTLTWIGQTEKIFQNQTEISNTALRHGVNNLYI
                     ALSGNTVMGDEEQVLLDWAEIKYWREYKTDQDYIKFTKPSNLPDGLYQFEVGGFSNAD
                     ISVYKIGSSIFNSVQIEPFNINGDAPWTVTLQDSVASDAVRYFAVTENKKKTPKNIRL
                     NFPSDLKNPYLSADVVVITPRKFTDVEGTLQLKSLWESEGHTVQIVDVQDIYDEFNFG
                     ITGSEPIRDFIRYAYNNWSSPQINQVILLGEGVDDTRDNSYSRMYNLIPVKKTWTYNH
                     GATASDQWYGCIVGTDNIPDVSIARISVWKEEQILAYAAKAISYRENLQTSRLWSSHL
                     TFTSGGKITDGNDLFAQQSERIIRKTIPPEYRVTRVYTSTQTVSSDYFGGTFNLKDAI
                     NSGTQYVQFMGHGGGRIWADYNLFNFNDVATLNNQVYPVVLSLACYCSAFDTNGMASI
                     SEALVLQPGKGAIGTAGFTGLGFRDHDEDWGLAYCEALFKHNFDNIGLANIYALARFY
                     STTYSPAARYALINGFAYMGDPLIKLRKPIKDIPVSANSYVLNPGDTLYVNADFPSGI
                     NAARLFIMKPSGKIVNVPYDLPVYPDGHFTASYVNPDQTTDNYFRKIMIAGYSPSDEF
                     VGISQYSVGRPNIVHHSFIPAEPAWSDSVLFTAKVFSPLPVQNIYCKVRTDSTSQVIN
                     WVDLPMQRSETDSTLYVTATRLGRQPTRKEIFYKYVLETEAGVSETILQSYVVAGPDL
                     LLKDIKLEQDGNNLVLKVLGTNVGNTPSITTDLKLYVGTTANNLAFFSSQSYPPLEVN
                     ELRWDSISLANLPNANLFLEVRVNTSNTFPEWEAYVNIGNYIRLQVPLNYYLIDSSGS
                     TLSSIDNNLQCTIPEGLVPTNEQILFAVNTLSALPPLNQPDVQNIMMNAPDGFSGNKY
                     SIPYEILALGTGVTDSLGVLNGGKKLFLSFNYSAIDSLTQAQENANNFRIYRYNSQVK
                     KWILIGGFVDVNLNKVQFEVDRTGIYSIFRNIDSSPPSIDVNVQDQEFTVGGYVAGNG
                     IISLLLSDANGIDVIDNSIQLFLNGIPIPEEDYVVSINLENINRIPIKYQLSLGRGIH
                     ELKVKCRDLNGMLATRDIQFTVNDKFDVINLANYPNPVLGTGGQGAAIDPKNEGRTRF
                     TYILTDGADEVTIKVYTISGRLVKTFRNLPVGVGYHEYPRTVYGWDCKDDSGYTLANG
                     VYFYRIIAKKGHKTIEKTQKMAILN"
     gene            5465..6475
                     /locus_tag="PKM80_02865"
     CDS             5465..6475
                     /locus_tag="PKM80_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92878.1"
                     /translation="MKKISILFSLTLMCLTPLFAGRYAGDFMMIGSGVRPLGMGGAFS
                     SLADDGSAIYWNPAGLCFITDSEVSAMHCFLYNNLASYDYVTYAQPLPNDVTICLNVT
                     RLTVSDIPHFDERYLIGTNVDQRINNSVYHLPGVPDSKFSSYDDLYQFAFSKKIHYDA
                     NMGWEFFAVPFDFGFGGTVKLIKRSIWDRTGTGTGIDIGFKAKTDLAVIFDWEDLGNL
                     DFGINFQDIGGTDITWDTPSKIRDEVLYNTKTGVAIEQPIPKLKSIVTLAYDYDYVYG
                     GTNHYGVDWEYDKKCNLRLGYYDTNFSAGATIKLYGVLLDYALITNPIGISNRLGLRV
                     RF"
     gene            6487..>6853
                     /locus_tag="PKM80_02870"
     CDS             6487..>6853
                     /locus_tag="PKM80_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92879.1"
                     /translation="MRKICILAGLLLLVLTYSCAADDSPEKDKIPPAAPDLLEHLGDT
                     GDDPITIDGIEVILDDDNNGIDAVPDGKWIKVPWKKFVDNDLSHVKVYRYTVQNPEPL
                     LIDTVPASEDYYLDRSFLIE"
BASE COUNT         2117 a         1143 c         1381 g         2212 t
ORIGIN      
        1 acttggtgga tatctgccag gccattttgc agagtgaaat aaataaaatt gagcttaaat
       61 tctcggataa aactgcttta ggagttgttt tagcttctgg aggttatcct ttaagttatc
      121 aaaagggctt tcccctcatt atgccttcaa ccctttccgg tatttatttt tctggagtag
      181 cagatgctga aaatggcctt gtaacttctg gcggacgggt tttatgtgct gttggtgttg
      241 ggaaagatct aaatgaagct agggaagagg catacagtaa agctcagcaa ataacttttg
      301 aaaacaggat ttttcgacaa gacatcggtt taaggaataa taccttatta gacaatggag
      361 acaaataata ttatgacaat atactctatt atacaggtta aaaacctgtc ccttgccaag
      421 ttttataagg caggtttatt attctatctt agttttttct tacctgctct tatatttgcc
      481 tcccctataa cacttatttc tgaaagtcag gatttactta ctattcaatt cgaacttcca
      541 gaatatgaac tggaagatgt aacaattaat ggccaaacct ggaaaagaat aatctgtagt
      601 gagagttcta tttatggtga agaaggttat ccagagctga aattattttc cacagcaata
      661 gctgttcctg tagatggaga tatcagtttc agcattgaaa gcagtgaatc tcaaacgcta
      721 aagaatgtga atatatatcc tgcggcaact atgttgctgg aagaaagtga accaatattt
      781 gttaataagc caaactataa agcatattct aatcgtgagc tgtatccact tcaagtagtt
      841 caaaaaggtg aacctgcttt tgttggtaac cgtagatttg ttcccttatt tatttgtcca
      901 tttcagtata gagctcaaag taaagaactc attatccaca gaagtattac tgtaagcgta
      961 tatgtagcag gttcaaaaaa tgcctccaag aattggcaac tgaatcctaa tcctcttgat
     1021 aatgcaactc catctttatt cttgaatgaa aacagctcca aaatatggcg cttggaaaaa
     1081 acccgcgata caaattatac ttcacccaaa aatggcactt ccgtaataaa tgaaattcaa
     1141 ataattgttg ataaagaagg tatttataaa gttgattacc attacctaat ggactatatt
     1201 aatatgatgg ttgattcctt acaggtaaat atgaattgga ctcctgccaa tgtagaccct
     1261 cgctatctgg aattgagtga tgaatatggt caggtaccta ttaattttgt tggtgaaaat
     1321 gatggtattt tcaatccgaa tgattatttt gaattttatg gtgatatgca taaaggggat
     1381 acttcctata tggatgatta cacttctgaa aatgtatata ctttgaaatt ggtggatcgc
     1441 tttggagcaa gaatggctgt agaaaacggt gggttgatag aatccaatat ctcaaattgt
     1501 attgttccag atgcttatga agatactgtt cacttcgagg aacaactgat aagcgataaa
     1561 ttgggtcagg gctggagctc aatagaccag aattactacc gagaagatgt ttggttctgg
     1621 agaaaaatag atgctcccga cttggaaatt gtacctatag aattgcaata tcctaaagat
     1681 actacaactc gctatgcctc tgctaaaata tgtttgatgg ggcttactta ttccgaaaca
     1741 ttaaccagtg gacaatttga ccacgaggca tctattcgtt tgaatcaagc aatgattaat
     1801 acccttacct ggattggaca gactgaaaag attttccaaa atcaaaccga aatctccaat
     1861 actgctttaa gacatggagt aaataattta tatatagctc tatcaggtaa tactgttatg
     1921 ggcgatgagg aacaagtttt attggattgg gcagaaataa aatattggcg ggaatataaa
     1981 acagatcagg attatataaa atttaccaaa ccttctaacc ttcctgatgg tttatatcag
     2041 tttgaagtgg gtggattcag taatgccgat atatccgtgt acaaaattgg ttccagcata
     2101 ttcaattctg tgcagataga gccctttaat attaatggag atgctccctg gacagttact
     2161 ttgcaagata gtgttgcttc cgatgccgta agatattttg ctgtaacgga aaataagaaa
     2221 aaaacaccga aaaacattag gttgaatttt cccagtgatt tgaaaaatcc ctatcttagt
     2281 gctgatgtcg ttgttattac tccccggaag tttactgatg tggaagggac tctgcaatta
     2341 aaatcactgt gggaatcaga agggcacact gtccaaattg tggatgtgca agatatctat
     2401 gacgaattta atttcggaat cactggtagt gaaccaatca gagattttat tcgttatgct
     2461 tataacaatt ggagcagtcc acaaataaac caggttattc ttttaggaga aggtgtagac
     2521 gatacacgag ataacagtta ttctcgaatg tataatctga taccagttaa aaagacctgg
     2581 acttataatc acggggctac agcaagtgat caatggtatg gttgtattgt cggaactgat
     2641 aatattcccg atgtatccat tgccagaatt agcgtttgga aggaagaaca aattttggct
     2701 tatgccgcta aggcaatttc ctatcgggaa aacctacaaa cctctcgtct atggagtagt
     2761 catttaacct tcacctccgg agggaaaatt accgatggaa atgatctttt tgctcagcaa
     2821 tcggaacgca ttatccggaa aacaattcct cctgaatata gagtaaccag agtttatact
     2881 tccacgcaaa cagttagctc ggattacttt ggcggaacct ttaacttgaa agacgcgatc
     2941 aattctggaa ctcagtatgt tcaatttatg ggtcatggag gaggaagaat ttgggcggac
     3001 tataatctct tcaattttaa tgatgtagca acgctcaaca atcaagttta ccctgtagtt
     3061 ttatctcttg cctgttattg ttcagctttt gatacaaacg gaatggcttc tatcagtgaa
     3121 gcattagttt tgcagcctgg taaaggagct attggaactg ctggttttac gggtttgggt
     3181 tttagggatc atgatgagga ctggggttta gcatattgcg aagcactatt taaacacaat
     3241 ttcgataata ttggtttggc aaatatctat gctttagcca ggttctattc cacaacttat
     3301 tctcctgcag ccagatatgc tttaattaac ggttttgctt atatgggtga tccgcttatc
     3361 aaattacgca aaccaataaa ggatattccc gtctcggcta atagctatgt tctaaatcct
     3421 ggggatactc tttatgtgaa tgcagatttc ccttcaggga ttaatgctgc gcgtttattt
     3481 ataatgaaac caagcggaaa aatagttaat gttccttatg atttaccagt ttatccggat
     3541 ggccatttta cagcgtctta tgttaatcca gaccaaacta ccgataatta tttccgcaaa
     3601 attatgatag ctggttattc tcctagtgat gaatttgttg gcatttctca gtatagtgtg
     3661 ggcagaccga atattgtaca tcatagtttt attcctgcag aacctgcctg gtcggattct
     3721 gttttattta ctgctaaagt atttagtccc ttgccagttc agaatatcta ttgtaaagta
     3781 agaaccgata gcacttcgca agtaattaat tgggtagatt tgccgatgca acgatcggaa
     3841 acggatagca ctttatatgt tacggctacg cgtttaggaa ggcaacctac tcgcaaagaa
     3901 atattctata aatatgtgtt ggagaccgaa gcaggtgttt cggagaccat tttgcaaagt
     3961 tatgtagtgg caggacctga tttactttta aaagacataa aattggaaca ggatggaaat
     4021 aacttagtat taaaagtgct gggaaccaat gttggaaaca ctccttcaat tactacagat
     4081 ttaaaattat atgtggggac tacggcaaat aaccttgctt ttttttctag ccaaagttat
     4141 cctcctttgg aagtaaatga attgcgttgg gacagtattt ctcttgccaa tttacccaat
     4201 gccaatttat ttttggaagt aagagtaaat accagcaaca cttttccgga gtgggaagca
     4261 tatgttaata ttggcaacta tatccgtttg caggttcctc tgaattatta tttgattgat
     4321 agttcgggtt ctactcttag tagcatagat aataatcttc aatgcacaat acctgaaggg
     4381 cttgttccca ctaatgaaca aattcttttt gccgtaaata ctttatcagc attacctccc
     4441 ctcaatcaac ctgatgtaca aaatattatg atgaatgctc cagatggatt tagtgggaat
     4501 aaatattcta ttccttacga gattttagca ttaggcacag gtgttacaga ttccttaggg
     4561 gttctaaatg gaggtaagaa actattcctt tcatttaact atagcgcaat tgattcttta
     4621 actcaggcac aggaaaatgc taataacttc aggatttatc gttataattc ccaagttaaa
     4681 aaatggatac taatcggcgg ttttgtagat gtaaatctaa acaaagttca atttgaggtt
     4741 gatcgaaccg gaatttacag tatcttcaga aatatcgata gttcaccacc aagtatagat
     4801 gttaatgttc aagaccagga atttactgtc ggtggctatg ttgctggcaa cgggattatt
     4861 tcgctattac tatctgatgc caacggaatt gatgttatag ataattccat tcaacttttc
     4921 ctaaatggaa ttcctattcc tgaagaagat tatgttgttt ccatcaattt ggaaaatatc
     4981 aatagaattc cgatcaaata tcagctatct ttaggacgcg ggatccacga actaaaagtt
     5041 aaatgtcgtg atttaaatgg aatgcttgca acccgcgata tccaatttac cgtgaatgac
     5101 aaatttgatg ttatcaacct tgccaattat ccaaatcctg tgttaggaac aggaggccaa
     5161 ggcgcagcta ttgatcctaa aaatgaagga cgcactcgtt ttacttatat attaactgat
     5221 ggagctgatg aagttactat caaagtatat actataagtg gacgtttggt taaaaccttt
     5281 agaaatttac ctgtgggggt tggttatcat gaatatcctc gtacagtgta tggttgggat
     5341 tgtaaagatg atagcggtta cactttggct aatggtgttt atttttatcg gataattgct
     5401 aagaaaggtc ataaaacgat tgaaaagaca caaaaaatgg caattctgaa ttaaggagct
     5461 taaagtgaaa aagataagta tcctgttctc gcttaccctg atgtgtctaa cgccactttt
     5521 tgcaggacgt tatgcaggag attttatgat gattggttcc ggagtgcgtc ctttgggaat
     5581 ggggggtgct tttagttcat tggcagatga tggttctgcc atatattgga atccggctgg
     5641 tttatgtttt attaccgatt cagaagtttc ggctatgcat tgttttctct ataataacct
     5701 tgcttcttac gactatgtta cttatgctca gcccctaccc aatgatgtaa ctatttgctt
     5761 aaatgtaact cgcttaacgg ttagtgacat tcctcatttc gatgaacgct atttgattgg
     5821 aaccaatgta gatcaacgga tcaataatag cgtatatcat ttgccgggtg ttccagacag
     5881 caaattttcc agttatgatg atctttatca atttgctttc tctaaaaaaa ttcattatga
     5941 tgctaatatg ggttgggaat tttttgcggt tccttttgat tttggtttcg gaggcactgt
     6001 taagcttatt aaaaggtcta tttgggatag gactggaacc ggaactggta tagatattgg
     6061 ttttaaagca aaaacagacc ttgctgttat ttttgattgg gaagatcttg gtaatttgga
     6121 ttttggcatc aattttcaag atattggagg aactgatatc acttgggata caccaagtaa
     6181 gataagagat gaagttttat acaatacaaa aaccggagta gcgattgagc aacccatacc
     6241 aaaactgaaa tctattgtaa ctttagccta tgattatgat tatgtgtatg ggggaacaaa
     6301 ccactatggt gtagattggg aatatgataa aaagtgtaat ttgcgccttg gatattacga
     6361 cactaatttt agtgcaggtg caactatcaa actttatgga gttttgctcg attatgcctt
     6421 aattactaat ccaataggta ttagcaatcg tttgggctta cgagttagat tctaaggagt
     6481 tctaaaatga gaaagatttg catattagcc ggccttttgt tacttgtctt aacctattcc
     6541 tgtgcggcag acgattctcc agaaaaggat aaaattcctc ctgcagctcc tgacttatta
     6601 gaacatttgg gtgatacggg agatgatccg attactatag acggaattga agtaatctta
     6661 gatgatgata ataatggtat tgatgctgtt ccagatggca aatggataaa agttccttgg
     6721 aaaaaatttg tagataacga cttaagtcat gtaaaggtat atcgctatac tgtacagaat
     6781 cctgaaccat tgttgattga tactgttcct gcttcagagg attattattt agaccggagt
     6841 tttttgatag aaa
//
LOCUS       DAORHB010000059         6842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_10955_length_6842_cov_3.453219, whole genome shotgun sequence.
ACCESSION   DAORHB010000059 DAORHB010000000
VERSION     DAORHB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6842
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10955_length_6842_cov_3.453219"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKM80_02875"
     CDS             complement(<1..451)
                     /locus_tag="PKM80_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNV92880.1"
                     /translation="MNDLAAIILAAGKGTRMKSERAKVTMPLADKPMIQRVVNTALAT
                     KCNKVFIVVGYMKNNVIAALEDNERIEFVEQQEQLGTGNAVIVTEPMFTNYDQDVLIL
                     CGDVPLLTADTLIRLYEKHKEEQASCTILTAFLDDPGKYGRILRNNEG"
     gene            complement(444..947)
                     /gene="folK"
                     /locus_tag="PKM80_02880"
     CDS             complement(444..947)
                     /gene="folK"
                     /locus_tag="PKM80_02880"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNV92881.1"
                     /translation="MIYHLCLGSNLDDPNSQLDKAIYQLSTESGISILRKSSRIITKP
                     YGMLNQPDFVNQVLEIESNDNPQDLMENLLYIEMSMGRLRRIKWGPRNIDIDILLAGD
                     LVLDSRKSPLKKPWPQVIIPHPDLQNREFILRLLNELIPDAIHPVLHKTINELYYELL
                     AREEHNE"
     gene            complement(950..1327)
                     /gene="folB"
                     /locus_tag="PKM80_02885"
     CDS             complement(950..1327)
                     /gene="folB"
                     /locus_tag="PKM80_02885"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNV92882.1"
                     /translation="MKIKLNEMVFYGYHGVQEEERTLGQRFIVSVTLYTNSSIDKGIR
                     RLEDTIDYTKVYLEIKEIMESQQFYLLENCANTIADKLLADFPLLEKLTICIHKPSVP
                     IQGNLKSVEVILSRDRNNQKDNA"
     gene            complement(1324..1734)
                     /locus_tag="PKM80_02890"
     CDS             complement(1324..1734)
                     /locus_tag="PKM80_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNV92883.1"
                     /translation="MNAEIIEDSGIVTKVEGNCVTVEIKPGGGCKSCTMRGFCFKESE
                     PTKFEIITDLPLQVNDRVQLDISPVGRTLVSLLVFGVPIIFLFLGFIIASLWFIEFVS
                     ILIGFVAMGLSFRIVRLIDKKYGKRLKISVARKL"
     gene            complement(1731..2216)
                     /gene="rfaE2"
                     /locus_tag="PKM80_02895"
     CDS             complement(1731..2216)
                     /gene="rfaE2"
                     /locus_tag="PKM80_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNV92884.1"
                     /translation="MLHTKVIPYNQIAELSENLHKQGKRIIFTNGCFDILHLGHILYL
                     EKAKAMGDVLIVGVNSDASVKRLKGNERPIIPENERALVLAALEMVNYVCIFEQDTPY
                     ELIDLIKPNVLVKGGDWPIEKIVGADIVQKAGGKVYSLNFIAGLSSTNIIERIKQGTQ
                     Q"
     gene            complement(2203..3195)
                     /gene="rfaE1"
                     /locus_tag="PKM80_02900"
     CDS             complement(2203..3195)
                     /gene="rfaE1"
                     /locus_tag="PKM80_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNV92885.1"
                     /translation="MHSIIPEKLEEILESFKHRHIIVLGDIMLDEYLWGKVQRISPEA
                     PVPIIEVNSADYRLGGAANAALNLKVLGAQVSLVGVCGKDTQATTIITQLKDKEMSTS
                     GIFLDSSRPTTLKTRIGSVSQQIVRLDREDVSELDENIQKEIYAYLTKIMPSCDALLI
                     EDYNKGLLSFDLINDVLQLARKHNKLVAVDPKYLNFSSYGGVEIFKPNYRELESYYGR
                     IFTDEDDFERSAEELRQKMNVKYLVVTKGSDGMYLFTGNKAVKHLPSFAREVYDVSGA
                     GDTVISALTLAYLFNSDIESAALIANHSAAVAVAKKGTASVTTKEILDSFNAPY"
     gene            complement(3176..4564)
                     /gene="purF"
                     /locus_tag="PKM80_02905"
     CDS             complement(3176..4564)
                     /gene="purF"
                     /locus_tag="PKM80_02905"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNV92886.1"
                     /translation="MCGIIGVYGNNNAARVAAVGLFAEQHRGQESCGMAVGDGFLIRL
                     HKKMGLVKEVFHNEQLSALPGNIAIGHVRYPTKGSATEFNTQPHLVETLSGPCYALAS
                     NGDIVNYAQVRKALEAEKVYFKSDNDGELLVKYIAYRLLHYQEEITDAIRHLMKDILG
                     AYSSVLCTPTELYMFRDPYSIRPMTWGKTPEGTIIVASESCALDTLGVTDRQEVPPAG
                     IIKVNEKGVQILENDPNLYRINKCEKHCIFEQIYFSRPDSFHFGENVYQVREKIGAAL
                     ARQDEGLNPDLVVPVPDSSNFIGLGYANQSGIPLSLGLIRNHYIGRTFIKPEQTVRDE
                     SVRQKFNVLPNFFNGKRIVLIDDSIVRGTTIRKIVDLIKEAGAAEIHLRIGSPQIKHS
                     CFYGIDTPTKHELVANRRTLDEIRDITGVDTLVHLSLRDLQSCVHCPEHYCYACFDGN
                     YPIGVPDALNYT"
     gene            complement(4573..5205)
                     /gene="plsY"
                     /locus_tag="PKM80_02910"
     CDS             complement(4573..5205)
                     /gene="plsY"
                     /locus_tag="PKM80_02910"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNV92887.1"
                     /translation="MNYPKFLPWIFLIAAYLIGSIPNGLIVSKLLHRKDIRKEGSGNI
                     GATNALRAFGTVTGLFVLLLDVAKGYFPTLLAMSILGKDNSFVSIIALFVILGHMFPV
                     YLKFKGGKGVATAAGVFLAIAPISLSMAILVFLIVVVLFHYVSLASICAAMAFHTHSL
                     AILLKAEKADYATLLLISVAVLLIVVKHKSNLERLINGSESKLKFTKKGN"
     gene            complement(5206..6543)
                     /gene="der"
                     /locus_tag="PKM80_02915"
     CDS             complement(5206..6543)
                     /gene="der"
                     /locus_tag="PKM80_02915"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNV92888.1"
                     /translation="MRKYIVSIVGRPNVGKSTLFNRLCRKRIAIVDFEAGVTRDRKYE
                     DVQWNGKEFKLVDTGGIVFDSTETIDKMVKHQALLAIEESDLIIFMVDAQTGATDIDR
                     DIAKILYPHRDKVMLVANKADNEKYEWELYDFLQLGLGDAFPLSASHGRNTGDFLDAL
                     IERIPGTQNAYQSEDKPSATKIAVVGKPNVGKSSIANLLLGNPKLIVTDIPGTTRDSI
                     DSPFRYHNKDYVLIDTAGLRRKTRVNYGVEYFSNLRTIEAVDRADIVVLVLAADEELS
                     VQDIKIASYAKRKMKEILVVYNKWDLVEKETNTVNMYLQNLHTQMPFLQFAPVLFISA
                     KTSQRINRIMETVTKIQEESEKRIPTSELNRFMETVVMHRPPTHPSGKHIKIFYITQA
                     QIKPPTFIFFCNTPSLITENYRRFLHNQLREAFKFEGVSIKLIFKGRKKEEED"
     gene            complement(6588..>6842)
                     /locus_tag="PKM80_02920"
     CDS             complement(6588..>6842)
                     /locus_tag="PKM80_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNV92889.1"
                     /translation="MLQTSIAVPSNIAMSVQIEDHNIDIEYLEIAFMKLRELETQLIV
                     ANDISYLKAEQTDKCFALINLIRSQISGYFNDHKVNERTV"
BASE COUNT         2124 a         1375 c         1106 g         2237 t
ORIGIN      
        1 cgccttcatt attgcgtaaa attcgtccat attttccagg gtcatctaga aaggcagtta
       61 aaattgtgca ggatgcctgt tcttctttat gcttttcata aagtcggata agtgtatcag
      121 cagtaagtaa cggaacatct ccacataaaa ttagaacatc ctgatcataa ttagtaaaca
      181 taggttcggt aacgataacg gcatttcctg ttcctaattg ctcttgttgt tccacaaatt
      241 ctatcctctc gttatcttca agagcggcta taacattatt tttcatataa ccaactacaa
      301 taaagacctt gttacacttc gtagctaaag cagtatttac cactctttga atcattggct
      361 tatctgcaag tggcatagtc actttagcac gttcggattt catccgagtc ccttttcctg
      421 cagccagaat tatcgctgcc agatcattca ttatgttcct cccttgctag taattcataa
      481 tataattcat taatggtttt gtgtaaaacg ggatggatag catcaggaat aagttcattt
      541 aataaacgta agataaattc ccgattttgt aaatccggat ggggaataat aacctggggc
      601 cagggttttt ttaatggaga ttttcggcta tccaaaacta aatcacctgc taaaaggata
      661 tctatatcta tattacgagg gccccattta atccttcgca aacgtcccat agacatttca
      721 atatataaaa gattttccat taagtcctgc gggttatcat tactttcaat ctcaagcacc
      781 tgattaacaa aatccggctg atttaacatt ccataaggtt ttgttattat tcggcttgat
      841 ttacggagaa tgctgattcc agattcggta gacaattgat agatggcttt atccaattga
      901 ctattggggt cgtctaaatt ggatccaaga caaagatgat agatcataac tatgcattat
      961 ccttttggtt atttctatcc ctgcttagga ttacttccac agattttaaa ttaccttgaa
     1021 taggtacaga gggtttgtga atacatattg ttagcttttc cagtaaagga aaatccgcaa
     1081 gtaatttatc tgctatggta ttagcacaat tttccagcaa atagaactgc tggctttcca
     1141 ttatttcctt tatttctaag tacactttgg tataatctat agtatcttcc aacctccgaa
     1201 tacctttatc tatagaggaa ttggtatata aggttacact aacaataaat ctttggccta
     1261 aagtccgttc ttcttcctgg actccatgat atccatagaa taccatttcg ttaagtttaa
     1321 ttttcacaat ttcctcgcaa cgcttatttt caagcgtttg ccatattttt tatcgattaa
     1381 ccgtactatt ctgaaggata aacccatagc aacaaaacct attaagatag acacaaattc
     1441 gatgaaccat agcgacgcta tgataaaacc caaaaataaa aatattatag gtacaccaaa
     1501 gactaacaag gacaccagag ttctacctac aggagaaata tctaattgaa ctctatcgtt
     1561 cacttgtaag ggaagatcgg ttatgatttc aaatttcgta ggctcacttt ctttgaaaca
     1621 aaaaccacgc attgtacaac ttttacagcc gcctccaggt tttatttcta ctgtaacaca
     1681 gttaccttca acctttgtaa caataccgct atcttcaata atttctgcat tcattgttgt
     1741 gtaccttgtt taattcgctc tataatatta gtactgctta agccggcgat aaaattcaag
     1801 gaataaactt ttcctcctgc tttttgtaca atatcagcgc ctacgatttt ttctataggc
     1861 caatctccac ctttaactaa cacattgggt ttaatcagat ctatcagttc gtaaggtgta
     1921 tcctgttcaa agatacatac ataatttacc atttccaaag ctgccaaaac aagggctcgc
     1981 tcattttctg gaataatagg acgctcattt ccctttaggc gttttacgga ggcatcacta
     2041 ttaactccaa caatcagaac atctcccatc gctttggctt tttctaagta tagaatatgt
     2101 cccagatgta aaatatcaaa acacccatta gtaaatataa tccgttttcc ctgtttgtgc
     2161 aagttttcac ttagttcggc aatttgattg taaggaataa ctttagtatg gagcattaaa
     2221 gctatccaag atctcttttg tagtaacact tgcagtgcct tttttggcta cagcaactgc
     2281 agctgaatga ttagcaatca aggcagcgga ttctatatcg gaattaaaaa gataagctaa
     2341 agttaaagca ctgataactg tatctcctgc accagaaaca tcataaactt cacgagcgaa
     2401 acttggtaga tgttttactg ctttattccc tgtaaataag tacattccat cactaccttt
     2461 ggttactacc agatatttaa cattcatttt ttggcgtaat tcttctgcag acctctcaaa
     2521 atcgtcttca tccgtaaata tccttccata ataactttcc agttcacggt agttaggctt
     2581 aaaaatttca actccgccat atgaactgaa atttaagtac ttagggtcta cagccaccag
     2641 cttattatgt ttcctggcta actgtaaaac atcatttatc agatcaaaac ttaacaaacc
     2701 tttattataa tcctcaatca ataatgcatc acatgatggc attattttgg taagatatgc
     2761 ataaatttct ttctggatat tttcatcaag ctctgaaaca tcttctctat ctaaacgaac
     2821 tatttgttga cttacagagc cgatacgggt ttttaaagta gttggccgtg aagaatccaa
     2881 aaaaatccct gaagtggaca tttccttatc tttcagctga gtaattatag tagttgcttg
     2941 agtatctttt ccacatacgc caaccaaact tacttgggca ccaagaactt taagatttaa
     3001 tgctgcatta gcagctcccc ctaaacgata atcggcagaa tttacttcta ttataggaac
     3061 aggggcttct ggagaaatgc gttgaacttt tccccataaa tattcatcga gcataatatc
     3121 gccaagtaca ataatatgac gatgtttaaa actctccagg atctcttcaa gtttttcagg
     3181 tataattgag tgcatcagga actcctatag gataattgcc gtcgaaacaa gcataacaat
     3241 aatgttctgg gcagtggaca cagctttgta aatcacgcaa tgataaatgt accagagtat
     3301 ccacaccggt tatatcacga atttcatcaa gggttcgtcg attggctacc aattcatgtt
     3361 ttgtgggtgt atcaattcca taaaaacaac tgtgcttaat ttgagggctg ccaatgcgta
     3421 aatgaatttc tgcagctcct gcttctttaa tcaaatccac aattttgcga atagtagttc
     3481 ctcgaacaat gctatcatct attagcacaa ttcttttgcc attaaagaaa ttcggtagga
     3541 cattgaattt ttgacggaca ctttcatcgc gaaccgtctg ttcgggttta ataaatgttc
     3601 tgccaatata gtggttacga attaaaccta aagataaagg tattccactt tgattagcat
     3661 agcctaaacc gataaaattg gatgaatcgg gaaccggaac taccagatca ggattcaatc
     3721 cttcgtcttg tcttgctaaa gctgcaccta ttttttcacg aacttgatat acattttctc
     3781 caaaatgaaa actatcggga cgggagaagt atatctgttc aaagatgcaa tgtttttcac
     3841 atttattaat gcgatagaga ttgggatcat tttccagaat ctgtacaccc ttttcattca
     3901 ctttaattat tccggcaggg ggtacttctt gtctatctgt aactcctaaa gtatccaaag
     3961 cgcaactttc tgaggctaca ataattgtcc cttcgggtgt ttttccccaa gtcataggac
     4021 gaatactata aggatctcta aacatatata attctgtggg tgtgcataaa acactggaat
     4081 aggcacccaa gatatctttc atcaaatgcc tgatagcgtc tgtgatttct tcctgataat
     4141 gcaataaacg ataagcaata tattttacca gaagttcacc atcgttatca cttttaaaat
     4201 atactttctc agcttccagt gcttttcgca cttgagcata atttacaata tcaccattgg
     4261 aagcaagagc atagcagggt cctgatagtg tttccactaa atgcggttgt gtattaaatt
     4321 ccgttgcact tcctttagta ggataacgaa catgcccaat agctatattt cctggtaaag
     4381 ctgaaagctg ctcattatga aaaacctctt ttaccagtcc catcttttta tgcaaacgga
     4441 taagaaatcc atcaccgact gccataccac aactttcctg tcctctatgc tgttctgcaa
     4501 aaagacctac agcagcaaca cgggcagcgt tattatttcc ataaacgccg attattccac
     4561 acattttagg ttctaatttc cttttttggt aaatttaagc ttagattcag acccatttat
     4621 taaacgttct agattgcttt tatgcttcac cacaattagt aaaacggcta ccgaaatcaa
     4681 aagtagggtt gcataatctg ctttctctgc ttttagtaat attgctaaac tatgtgtatg
     4741 aaaagccata gctgcacata ttgaagctaa agaaacataa tggaaaagca caacaacaat
     4801 caaaaaaaca agaatagcca ttgataaact gataggtgct atggctaaaa aaacacctgc
     4861 tgctgtagct acacctttgc cacctttaaa ttttagatag acaggaaaca tatgtcccaa
     4921 aattacaaaa agtgcaatta tggatacaaa agagttatcc tttcctaaaa tagacatagc
     4981 taacagcgtt ggaaaataac ccttggctac atccaaaagg aggacaaaca agccggtaac
     5041 agttccaaaa gctcttaaag cattggtagc tccaatattt ccactacctt ctttacggat
     5101 atcttttcta tgcaataact tgcttactat caatccgtta ggaatactac ctatcagata
     5161 agcagctatc aaaaatatcc agggtaggaa tttgggataa ttcatttaat cctcttcttc
     5221 ttttttgcgc cccttaaaaa taagtttgat gctaactcct tcaaatttga atgcttcacg
     5281 caattggtta tgaagaaatc tgcggtaatt ttcagttatc aacgaagggg tattgcaaaa
     5341 gaaaataaaa gtaggtggtt ttatttgtgc ctgcgttata taaaatattt ttatatgttt
     5401 tccacttgga tgggtgggag gtctatgcat aactacagtt tccataaaac gattcaattc
     5461 agaagtagga atccgctttt cgctttcttc ttgaattttg gtcactgttt ccataatgcg
     5521 attaatccgc tggctcgttt ttgcagaaat aaaaagcaca ggagcaaatt gcaagaacgg
     5581 catctgtgta tgcaagtttt gtaagtacat atttactgta tttgtttctt tttctactaa
     5641 gtcccattta ttatacacaa ccagaatctc tttcattttg cgtttggcat agctggcaat
     5701 tttaatgtct tgaacgctaa gttcttcatc agcagcaaga actaaaacta ctatatcagc
     5761 tctgtctaca gcttcaattg tccttaagtt agaaaaatat tccactccat aatttactct
     5821 ggttttgcgt cggagtcctg ctgtatctat taaaacataa tccttattgt gatagcggaa
     5881 agggctatct atagaatctc ttgttgttcc tggaatatca gttacaatta attttggatt
     5941 acctaaaagt agattagcga tagaactttt acctacattg ggttttccca caacggcaat
     6001 ctttgtagca gaaggtttat cctcactctg ataagcgttt tgagtgccag gaatccgttc
     6061 aatgagagca tctaaaaaat caccagtatt acgaccatgg gaagcagaaa gaggaaaagc
     6121 atcacctaaa cccaactgca aaaaatcata cagttcccat tcatattttt cgttatcagc
     6181 tttattagca acgagcataa ctttgtctcg atgtggataa agaattttgg caatatctct
     6241 atcaatatct gtagccccag tttgagcatc taccataaat attatcaggt cgctttcctc
     6301 gatggctaaa agtgcctggt gtttaaccat tttgtctatg gtttcagtgc tgtcaaaaac
     6361 aattccacca gtatcaacaa gtttaaattc tttgccattc cactgcacat cttcatattt
     6421 acgatcacgt gttacgcctg cttcaaaatc tacaatagca atgcgtttac gacacaaacg
     6481 attgaacaat gtagattttc ctacattggg acggcctacg atagaaacaa tgtattttct
     6541 catattatct ttaatttcag caagaaagca gtattaatat aaacaggtta tacggttctt
     6601 tcattaacct tatggtcatt aaaatatccg gatatttgag agcggatcag atttatcagg
     6661 gcaaaacact tatccgtttg ttctgctttt agataactaa tatcattggc aacgataagc
     6721 tgagtttcca gttctctcaa tttcataaaa gctatttcca aatattcaat atctatatta
     6781 tgatcttcaa tttgtacact catagctata ttggaaggaa cggcaattga agtttgtaac
     6841 at
//
LOCUS       DAORHB010000060         6768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_11127_length_6768_cov_5.112319, whole genome shotgun sequence.
ACCESSION   DAORHB010000060 DAORHB010000000
VERSION     DAORHB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11127_length_6768_cov_5.112319"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PKM80_02925"
     CDS             complement(<1..1431)
                     /locus_tag="PKM80_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92890.1"
                     /translation="MKKTLKIIFLTVVALSFVIVVFAQNSNEATKKEIIQQISQERQI
                     LKEIAKNFLIDVLPELVKKDPALAEQALVKYKDAYSSLGQNDIVYLLGHFYTRMGENT
                     RAISYFNSLLQTNLNEDSRKMLNLALYQQMVHYLETGNRKAARDFLQVIIFDQYQFDT
                     YYPTYLYVWADMTAEEGQYETVQNTLQSYMQNRDIIMNRLLPQKIAILARVQNLDLDS
                     YYANPSGETYKQLASQIDVIKGDLTNNYNELMSLKGIIYVDAIVNLHNEEIGMLDSLK
                     IVLSDYYTKKIYAEETITRGTSLLQQIKKLSSSYERQLAIMDQILERQYELYKANDPQ
                     IQGNKYSDMELKRLYDLEKNIQLYTDLIEELDNYIADPKLVDMQAQLKIIRDDYDEKR
                     TSLNLRKEYLLQVRKHSSDAQEQIFNTILQEYYALYPDKKNLDKQIMELEQFMKEETQ
                     NIFTEEMRNKIKSNVASHIAQTADSNK"
     gene            complement(1587..2705)
                     /locus_tag="PKM80_02930"
     CDS             complement(1587..2705)
                     /locus_tag="PKM80_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92891.1"
                     /translation="MKKIILMAIAVVAVISSVQAAPFQTLGMLRTPDAYVLPNKAAEF
                     VLVGYYRDIAAPKPLTNDAKDASEGLFPYTMIGVGILDRVELGLFLGDYTSLDGLVYY
                     LNAKVKIIEETLRIPQVSIGMDNILSPVPKHGSQYLSPGDDFSQHPDADCYEPYSPYA
                     VASKQAVFLGIPWMFNVGIGTNRFVGQAKSRYLNGLFASAEMSPIKDLALQGEFDGED
                     LNIGAKYSYKNFGFKVGVNALEDWFKDSDYKDNIRIAFGVSYLFDKYAEAKRRPDLGH
                     YAGYGKTPSMGEGVVETPIETGITVPPTVSPSKPQAGTQTPAPGLTTSGTAGYEQLSP
                     EVQELMKELRMLKEDREKAQQAMDELRKWIEELKKQKQ"
     gene            2790..3176
                     /locus_tag="PKM80_02935"
     CDS             2790..3176
                     /locus_tag="PKM80_02935"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNV92892.1"
                     /translation="MIKYSLQNFSRIGEDLAARYLVTNGYSVVCRNYRKRCGEIDLIV
                     EKDQHLIFCEVKTRTSHSIESALSSVSFSKQRKITRAAQLYINENPNFGNYIFRFDVI
                     LVFYYESTDTFKVIHYEDAFPPLLDN"
     gene            complement(3173..4510)
                     /locus_tag="PKM80_02940"
     CDS             complement(3173..4510)
                     /locus_tag="PKM80_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNV92893.1"
                     /translation="MKNIRILICLALLSCFLIPLSGSEKLNMFIDYSRFLDKNKNTIL
                     LLDYQVPYQNLAFLAKNNGFFAELEVYIKICEGDSLLLDHKINDLIGVRNKADTSNKQ
                     KSYLNRFSFIMDRPICTVQFYAQDLNSEKSFMHEFEITALPPNSLLSDIELNSEVRAD
                     TTSYLPKFKRNNILYRSEPSILFNKTNTDYVYLYLESYIGSTSLESCLLNLSLEKDSV
                     IVMDEDLNYKPASSSESINLKIPISDLNPGLYLGTVILQNPDQYEERKFEFAVIEEKE
                     EEFFLFANPDDEFELMRIFMGNRISADWKDLSQDSKKRYITQFWRNMASNTNRSIQSI
                     MDLVQERIDYSNKHFGHFKKGWTSDMGRIYIRNGAPDDIQRETSSDESRFVRKDYQIW
                     KYSTGNKPVYLFIDIQMNGNYKLIYVVNDDMESSNPDWMRYVGYDFDTSKLDN"
     gene            complement(4497..5603)
                     /locus_tag="PKM80_02945"
     CDS             complement(4497..5603)
                     /locus_tag="PKM80_02945"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNV92894.1"
                     /translation="MEKVLSILILLFTFGFVSAQEIFYEHYANQVDIRVVLPYNNISF
                     KKGSDQAVYQIVLEISNSRKKQVATLEKSQIIPKYEWLTDCGIPVFFQLELPQGSYNV
                     ALKIKNKAMGDKRNYTRQFIIGPSSTEIGLSWVIAKKEGIDFIPYSLSGLSVDELCLN
                     QSYSLALDSLRIQIDNQNLTITNPQSPVIIDLLPYIQKDAPNQLALTFFENNIYYKME
                     PFLFSPWYAYSLRYSLNDQIRQIRYIANQNEWNVLRNVPEERQAEVIEAFWKANDPSP
                     GTIRNELRERFYQRVLAADELFTVHKKIAGWTTDQGRIYIKYGEPDDVYSEVFSIGKY
                     PFIIWYYYDKNLEFIFADIGGYGQYTLRNKDEEY"
     gene            complement(5587..>6768)
                     /locus_tag="PKM80_02950"
     CDS             complement(5587..>6768)
                     /locus_tag="PKM80_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNV92895.1"
                     /translation="QTAYLKTYYPVEYMAALLSLEDDPSGIPGKIEVCKKMGIKIIPP
                     NINRSDSEFRVHNKEVQFGLRAIKNLGEAAMRAIIESRDQGGPYTGIFNFCSRLDSSS
                     VNKTILESLIASGAMDELEGTRAQKWAVIEQALQFSSNEQRDRKKGQSTIFDFLDEED
                     DNSNYYPPLPNVEPWGYLYQLEKEKEVLGFYLSGHPLYEYRSLIKHITNADSNTGKSN
                     NNGELYLAGIITNISKKKDSRGNQIAFVEFEDLAGKFEVSLFNKDYLQYSKIISPGKV
                     FFITGTKSTFNGNDDAMLRILPNSLIPFDALANELRGEIKVHINDKQVKKGCLTDLGE
                     RISRCDGKFKLITTIQKEDVNSYQLESRKLFFPGEGLLNWLEKEKIDFQIRLVTNGKS
                     T"
BASE COUNT         2000 a         1308 c         1126 g         2334 t
ORIGIN      
        1 tttattacta tcagcagttt gggcaatgtg actagccaca ttacttttga tcttattacg
       61 catttcttcg gtaaagatgt tctgggtttc ttctttcata aattgctcaa gttccattat
      121 ctgtttatcg agattcttct tatcaggata aagtgcataa tactcttgca ggatagtatt
      181 gaatatttgt tcttgagcat cacttgagtg tttacgaact tggagtaggt attctttgcg
      241 aagatttaaa ctcgtgcgtt tctcatcgta atcatcacga ataattttta gttgtgcttg
      301 catatctacc agtttaggat cggctatata attatccaat tcttcaatta gatcggtata
      361 aagctgaatg ttcttctcta aatcgtaaag acgtttcagc tccatatcac tatatttatt
      421 accttgaatc tggggatcat ttgccttgta tagctcatat tgtctttcca gaatttggtc
      481 cattatagca agctgtctct cataactgga tgatagtttt ttaatttgtt gtaataaact
      541 tgttcccctc gttattgtct cttctgcata tattttcttg gtataatagt cacttaaaac
      601 aattttcaaa gaatcaagca taccaatttc ttcattatgc agatttacaa tcgcatctac
      661 atatataatc ccttttaagg acatcaattc attatagtta ttagttaaat cccctttgat
      721 aacatctatt tgggaagcca gttgcttata tgtttccccg cttggatttg catagtagga
      781 atctaaatct aagttttgaa ctcttgctaa tatagctatt ttttgaggaa gaaggcgatt
      841 cattataatg tctctattct gcatatagct ttgtagagta ttttgcacag tttcatattg
      901 gccttcttca gctgtcatat cagcccaaac atatagatat gtaggataat atgtatcaaa
      961 ctgatattga tcaaaaataa tgacctgtaa gaaatccctg gcagctttac ggttcccagt
     1021 ttccagataa tgcaccattt gttgatataa agccaaatta agcattttcc gagaatcttc
     1081 gttcaggttt gtttggagca aagagttgaa ataactaata gctcgcgtat tttcccccat
     1141 acgcgtatag aaatgaccaa gtaaataaac aatatcgttt tgtcctaaac tggagtatgc
     1201 atctttgtat ttcactaatg cttgttctgc cagtgccggg tcttttttga ccaattcagg
     1261 taaaacatca atcagaaaat ttttggctat ttcttttaat atttgtcgtt cctgagatat
     1321 ttgctggata atttcttttt tagtagcctc attgctattc tgcgcaaaga ctactataac
     1381 aaacgacaaa gcaacaacgg tgaggaatat aatctttagt gtttttttca taatgctgct
     1441 cacttaattt tccttcctta ctctttccat taggggccaa agttcaggat tctctgcttc
     1501 agcaaagtaa tcctgaactt ttttatttct ttatgggaaa cataatgcct caacctgtcc
     1561 attctgtagg agcagattaa gcagaattac tgtttttgtt tcttcagttc ttcaatccat
     1621 ttgcgcagtt catccatcgc ttgctgagcc ttttccctat cttccttcag catccgtagt
     1681 tctttcatca attcctgaac ttcaggagaa agttgttcgt aacccgcagt tccagatgtg
     1741 gttaaaccag gggcaggagt ttgagttcca gcttgtggtt tacttgggct aactgttgga
     1801 ggaactgtaa ttccagtttc gattggggtt tcaactaccc cttcccccat agagggtgtt
     1861 tttccgtaac cagcataatg accaaggtcg ggacggcgtt tggcttctgc atacttatcg
     1921 aacagatatg aaacaccaaa agcaatccgt atattatcct tgtaatcgct atccttgaac
     1981 caatcctcaa gagcatttac accaacttta aagccaaaat tcttatagga atatttggcc
     2041 ccaatattga gatcttcacc atcaaattct ccttgtaatg ccagatcttt aattggagac
     2101 atttctgctg aagcaaatag tccatttaaa tagcgggatt tagcttggcc aacaaaacgg
     2161 ttggtgccaa ttcctacatt gaacatccag ggtattccta aaaatacagc ttgcttggat
     2221 gctaccgcat aaggagaata gggctcgtag caatcagcat caggatgttg actaaaatcg
     2281 tcaccagggc ttaagtattg agagccatgt ttaggaacag gggataaaat attatccatt
     2341 cctatagaga cctggggaat tcgtaaggtc tcctcaataa tttttacttt tgcattaaga
     2401 taataaacca atccgtctaa ggaagtgtaa tcacccagaa ataaaccaag ttcaactcta
     2461 tccaaaatac ctactccaat cattgtataa ggaaataaac cttcggaggc gtctttagca
     2521 tcgttagtaa gtggtttggg ggcggcaata tctctgtaat aaccaactaa tacaaattct
     2581 gctgccttat tgggaagaac ataggcatct ggagttctca acattcctaa agtttgaaag
     2641 ggcgctgctt gaacactact aatcacagca actaccgcaa tcgccatcaa aataattttt
     2701 ttcatgtttc cctccacctt tcggtgttaa ttacttatgt tgtccatcat tctcattgac
     2761 gaaatatccg tcaagttttt tcttgagata tgattaaata ttcacttcag aatttttctc
     2821 gtattggcga agatcttgca gcgcgttatt tagtaactaa cggttattca gttgtttgcc
     2881 gtaattaccg taagcgctgt ggggaaatag atcttattgt ggaaaaagat caacacctaa
     2941 tattctgcga agttaaaacc cgtacttctc actctatcga gtctgctctg tcttctgttt
     3001 ccttttccaa acaaaggaag ataacaagag cagcacaatt atatattaat gaaaatccaa
     3061 actttggcaa ctatattttt cgttttgatg ttatcttagt attttattat gaatctacgg
     3121 atacattcaa ggttattcat tacgaagatg cctttccacc gctgttggac aattaattat
     3181 ccaatttgct agtatcaaaa tcgtagccga catagcgcat ccagtcagga tttgagcttt
     3241 ccatatcatc gtttactaca taaatgagtt tataatttcc attcatttga atatcgataa
     3301 acagatatac tggtttattt ccagtagaat atttccagat ttgataatcc tttctaacga
     3361 agcggctttc atcagaactt gtttctcttt gaatatcatc gggagctcca ttccttatat
     3421 atatgcgtcc catatctgaa gtccatcctt ttttgaaatg tccgaaatgt ttattagaat
     3481 aatcgattcg ttcttgcact aaatccataa tgctttgtat gctgcgattt gtgttggatg
     3541 ccatatttct ccaaaattga gttatatacc gctttttact atcctggctc aaatctttcc
     3601 aatctgcaga aattctgttt cccataaata tccgcataag ttcaaattcg tcatcaggat
     3661 tggcaaagag gaaaaattct tcctcttttt cttcgatcac ggcaaattcg aattttcttt
     3721 cttcgtattg atcaggattt tgcaagataa cagtccccag atataaacct gggttaaggt
     3781 cagaaatagg tatttttaaa ttaatacttt cgctgctgga ggcaggttta taattcaaat
     3841 cttcatccat cactattaca gaatcctttt ccagggataa attcaggagg cagctttcca
     3901 aagaagtact tccaatatag ctttccaggt aaagataaac ataatcggta ttagttttat
     3961 taaagagaat ggagggctcg ctacgatata ggatattatt tcttttaaat ttgggcaagt
     4021 aggaagtagt atcagctcgt acttcagaat tgagttcaat atcgcttaat aaactgtttg
     4081 gaggcaaagc ggtaatctca aattcgtgca taaaactttt ttcggaattt aggtcttgag
     4141 catagaattg gacagtgcag atgggtctat ccataataaa actgaagcga ttgagatatg
     4201 atttttgttt attagaagta tcagctttat tgcgaactcc aatgaggtca ttaattttat
     4261 ggtctagcaa caaactatca ccttcacaga ttttaatata tacttccagt tcggcaaaga
     4321 atccattgtt tttagcaaga aaagccagat tctgataggg tacctgataa tcgagcagta
     4381 atatagtatt tttattttta tccaggaacc tggaataatc aataaacata ttcaattttt
     4441 ctgatcccga tagcggaata agaaaacagc tgagaagtgc caagcaaatt agaattctaa
     4501 tattcttcat ctttattcct taaagtatat tgaccataac ctccaatgtc agcaaagata
     4561 aattcgaggt ttttgtcata gtaataccag attataaaag gatatttccc tatagagaaa
     4621 acttctgaat agacatcatc gggttctcca tattttatat atattctacc ctgatcagtc
     4681 gtccatcctg caattttttt atgcactgta aaaagttcat ctgctgctaa aaccctctga
     4741 tagaatcttt cccgcaattc atttctgata gttccagggc tgggatcatt ggctttccaa
     4801 aatgcctcaa ttacttcagc ctgtctttct tcaggaacat ttcgtaaaac attccattca
     4861 ttttgattgg ctatatagcg aatttgtctt atttgatcat tcaaagaata gcgcagcgaa
     4921 tatgcatacc aaggggaaaa tagaaaaggt tccatcttgt agtagatatt gttttcaaaa
     4981 aaagttaaag ccaattgatt cggtgcatct ttttggatgt aaggaagtaa atcaataatt
     5041 actgggcttt ggggatttgt tatagttaag ttctgattat caatttggat acgcaagcta
     5101 tccaaagcca aagaatagct ctgatttagg catagttcat ccacactcaa tccggataaa
     5161 ctatagggga taaaatcaat accttccttt ttagcaataa cccaactaag cccaatttcc
     5221 gtactggaag gacctatgat aaattggcga gtataatttc ttttatcccc catcgcttta
     5281 ttttttattt tcaaagctac attatatgaa ccttggggaa gttccaactg aaagaaaact
     5341 ggaatgccac aatcagtgag ccattcatat tttggtataa tttggctttt ttccaaggta
     5401 gcaacctgtt ttttccggga attagaaatc tcgagcacaa tttggtatac tgcttggtcc
     5461 gaaccttttt tgaagctaat attattgtaa ggcaaaacaa cacggatatc aacctggttt
     5521 gcataatgct catagaaaat ttcctgagca gatacaaagc caaaggtaaa tagtagaatc
     5581 aggatactaa gtactttttc catttgtaac caatcttatt tgaaaatcaa ttttttcttt
     5641 ttccagccag tttaaaagtc cttccccggg aaagaataat ttacgggatt ccagttgata
     5701 actattcaca tcttctttct gaatagtagt aatcagttta aatttaccat cgcagcgaga
     5761 tattctttct cccaaatcgg ttaagcaacc ctttttcact tgtttgtcat tgatgtgtac
     5821 ttttatttcc cctcttagtt cattggctaa agcatcaaag ggaattagag aattgggcaa
     5881 aattctcagc atagcatcat cattaccatt aaaagtggat ttagtgcccg ttataaaaaa
     5941 gacctttccc gggctaatta tctttgaata ttgcaaataa tccttattaa acaagcttac
     6001 ttcaaattta ccggcaaggt cttcaaattc cacgaaggca atttgattgc cccgactatc
     6061 tttctttttg gaaatattag taataattcc tgcaagatat agttctccgt tattgttgct
     6121 tttgccagta ttggaatcag cattagttat atgtttaatc aaagacctgt attcatacaa
     6181 aggatgaccg ctcaaataga aacctaaaac ctctttttct ttttccagct gatataaata
     6241 gccccaaggt tcaacattag gcagaggagg ataatagtta ctgttatcat cttcctcatc
     6301 caaaaaatca aatatagtgc tctgtccctt ttttctatct ctttgttcat tactgctaaa
     6361 ctgtaatgcc tgttcaatta ctgcccattt ttgagctctt gttccttcca gttcatccat
     6421 cgctccggaa gcaattaaac tctccagaat ggttttattt acgctgctgc tatctaagcg
     6481 actgcaaaaa ttgaagatac ctgtataagg tcccccttga tctctacttt ctataatagc
     6541 tcgcatagct gcttccccca aattttttat agctcttaaa ccaaattgga cttctttatt
     6601 atgcacccga aattcactat cactgcgatt aatattggga gggataattt ttatccccat
     6661 ctttttacac acctcaattt ttccgggaat tcctgaagga tcatcttcca gagatagtaa
     6721 cgcagccata tattctacag gatagtatgt tttcagatag gcagtttg
//
LOCUS       DAORHB010000061         6705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_11292_length_6705_cov_5.935639, whole genome shotgun sequence.
ACCESSION   DAORHB010000061 DAORHB010000000
VERSION     DAORHB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11292_length_6705_cov_5.935639"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /gene="guaB"
                     /locus_tag="PKM80_02955"
     CDS             complement(<1..1322)
                     /gene="guaB"
                     /locus_tag="PKM80_02955"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNV92896.1"
                     /translation="MNLHKAYTFDDVLLLPQQSEVLPSEVDLTTNITSNISLRIPVIS
                     AAMDTVTESAMAIAMAREGGIGIIHKNLSIEEQARQVSLVKRAESGVVTHPYILAPED
                     TLEDVIALKEAHHIGGFPVVENDFLVGILTNRDFRFETNPNKKVKEMMTPKEQLITAK
                     TGISMDSAIDLLQEHRIEKLLLISDDGKLEGMITVKDIMKRLNYPEAVQDDKNRLLVG
                     AAIGVKGDYLERAQELVKAGADLLVIDTAHGHHKNIGIALQNVKKHLNCQLIAGNVAT
                     AKACRYLIDNGADAVKVGIGPGSICTTRVIAGIGVPQLSAIMDCALEANKNSIPIIAD
                     GGIKFSGDIVKAIAGGASAVMIGSLFAGCDESPGESIIYNGRRFKSYRGMGSLSAMKL
                     GSKDRYFQDEQLEENKLVAEGIEGMVPYKGPLKDFLYQLIGGLRSGMG"
     gene            complement(1342..3069)
                     /locus_tag="PKM80_02960"
     CDS             complement(1342..3069)
                     /locus_tag="PKM80_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV92897.1"
                     /translation="MKKIALLLLILITCESAIYAQYDEKQILTQQANQLLIQRQYAQA
                     EQLFLQILDKYPNDLNSINQLMNLYFTLSQTDKAETTLKKYQRILPPQTYSEFYIQLL
                     VTQAKVDDAWQEAMRHLANFGYDEFKFRRLASFFERKAFYDKVMELYTMARNRLGKPD
                     LFRLEMANTALNCRQFEKAIREYMAYTESNPVYLFFTNNQLKTILQEEPSLISVVSAI
                     ADSSKSSVLKELYANALVNMKDYKTALSIYKQLDPQKMWHFAEDQAAVGNDSIAFEAY
                     SYLENIETDQVKKMDLLFRMAELKYQQRDYPGSEQIISGCLSMPFWKDRNLYYRSNVS
                     VKLRKLMAEIMLAKNEPVDSALVWLNEAKKFSRNNLEAQEMDLEIARLYILNSEKEKA
                     DNILKTINYNTLMETRSYLNFLNTFLAGETALADTLMNEFIIRYPESKYTNDAIYLMM
                     FTLNMDEQDRSTFYSAVRLLQLNHPSGIDSLEVIFKHNKDEEILLLAIEWAIAFGDET
                     RAAALLQYDFQDEIASDYAALLRLLLVADRDEEQRMAKEFLKNKPNSVFSPDFRKKIS
                     RWATSKPNI"
     gene            complement(3080..6313)
                     /locus_tag="PKM80_02965"
     CDS             complement(3080..6313)
                     /locus_tag="PKM80_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92898.1"
                     /translation="MSELTKFIQKYRQKINLRFVLRAIIGSLLSFAVALHIYFLLWLN
                     LPSQSMALLYANIGIRTCLIMVIIYFLWSGYRNFWHNLSVARFLDKQIDYRDDLFQNT
                     LELAEKEKDSPIMENLSILAEHRIKENQYKIPALYPSWIVFAILFLIVGLGSIWANSF
                     SDFRLAFKQFYTNKGQEVIYKDYIELSPGNKTIGRNQPIEIKILNPDPRLQHHLFYRI
                     DKQWRELGLADNSYIFQRLDNSIEYYAQNEVCKSPIYKIQVLDEPIVKKWFVQYNPPA
                     YSGLTSWTDTLSYGNIEALKYSKVKLSFSTNIPVESAVMVFDDASRLPLQALDKSNYI
                     VQLSIEKPRTWYLELTDALGRQNKPEEKTISILEDNPPEIRITFPGEDVNLNQNMLLP
                     LIIEADDDYGLKNCALNIQIMASEPQVLNVQSVISGKMFTTDFLLDLKEANLFPGDVV
                     TYWATIYDNSPEQHKAESTRFKARFPSIEEIYREIETKEHEKKSELQTALEKSKDLQK
                     EFENKRRELLKKENLQWEDKKQLEQILQKQENLSEQVQNIADNYQELINKMQANETLS
                     PETLQKMQKIQELMQEINNEELQEAMRKFENALQNVKTEDLKKALENFKFSMEDFAKK
                     IDQTLALLESIKKEQALQKALQISEEMEKMQKTLSERTMDGKQNPKDLAADQKQISDT
                     YDKLQQELSKISEMLESPRDNDVIKQLNDLMQDMKNSNLKQDFQNSQNALNQNQRSES
                     QSAQNNALEKMRRFSLKLNKMKESMGGGNQQRMLQAVQTAIRELLIFSKQHEATTACY
                     RNDPYAIIHDLISQSEGIQISLNKLFSETQVLMMIPPKFFIDLNDTNNAYRNVFSSIN
                     ESQFYQIPNNLTFIQKGINLMVYDLMQALQNASSGSGSGGGMQSLLQMLEQMSQEQMA
                     MNMLTEQLMLQLQAQGGKMDSAMQQQIQRLAADQERLAENLKRALQNNPEAQKQGNVI
                     KQITEEMEAITRQLKNNQLNPELLERQERIISKMLDAQRSINKREFTEKRKAEAGQDI
                     PILGNTKIDLEALRRSGLLDEGLRAYPKEYQQVIMQYLKALNEATSK"
BASE COUNT         1911 a         1335 c         1123 g         2336 t
ORIGIN      
        1 tatcccattc cggaacgcaa gccacctatt aattgataga ggaaatcttt caagggaccc
       61 ttatagggaa ccattccttc aatcccttcg gcaacaagct tattttcttc aagctgctca
      121 tcttgaaaat agcggtcctt actacctaat ttcattgccg aaagtgaacc cattccgcga
      181 tagcttttaa atcttctgcc attataaata atggattcgc cagggctttc atcacaacca
      241 gcaaaaagtg aaccaatcat aactgcagaa gctcctccag caatagcttt cacaatatct
      301 ccactaaatt ttatgccccc atcggcaata ataggaatgc tatttttatt tgcttctaaa
      361 gcgcaatcca taatcgcact taattgggga actccgattc cggcaattac tctagttgta
      421 caaattgaac ctggacctat gccaacttta actgcatcag ctccattatc aattaaatag
      481 cgacacgctt tggccgtagc aacattgcct gctataagtt gacaatttaa gtgcttttta
      541 acattttgca aggcaatccc aatgttttta tgatgtccat gagcagtatc aataactaat
      601 aaatctgctc ctgctttaac aagttcttgt gctctttcca gatagtcacc cttaactcca
      661 atcgcggcac ctacaagtaa gcggttttta tcatcttgta ctgcttccgg atagtttagc
      721 cgtttcataa tatctttaac tgttatcatc ccttccagct taccatcatc actaattagc
      781 aacaattttt caatgcgatg ttcttgcaac aggtctatgg cactatccat agaaattccc
      841 gttttggcag tgatgagttg ttctttggga gtcatcattt ctttcacttt tttattcgga
      901 ttggtttcaa aacggaaatc gcggttggtt aaaataccta ctaaaaaatc attctctaca
      961 actggaaaac ctccaatgtg gtgagcttct ttcaaagcta taacatcctc taaagtatct
     1021 tctggcgcaa gaatataagg atgagtaact actccacttt cagctcgttt tactaaactt
     1081 acctgtcttg cttgttcttc aatggaaagg tttttgtgaa tgattcctat acctccttct
     1141 cttgccatag caatggccat agcagattca gttacagtat ccatagctgc actgataaca
     1201 ggtatccgca aagaaatatt ggatgtgatg tttgtggtta ggtccacttc tgagggcaaa
     1261 acttccgatt gttgaggaag cagcaaaaca tcatcaaaag tatatgcctt atgtaaattc
     1321 attttatctc ctctttattt tttatatatt tggtttagaa gtagcccagc ggcttatctt
     1381 ctttctaaaa tcaggtgaga aaacgctatt gggcttgttt ttcaaaaatt ctttagccat
     1441 ccgttgttct tcatctctat cggcaactaa taataaacgc aataatgcag cgtaatctga
     1501 agcaatttca tcttgaaaat catattgaag caaagctgct gcccttgttt catctccaaa
     1561 agcaatagcc cattcaattg ccagcagcaa tatctcttca tctttattat gtttaaagat
     1621 cacttccaaa ctgtcaattc cgcttggatg atttaattgc aacagacgca cagccgaata
     1681 aaaagtactt ctatcctgct catccatatt cagcgtaaac atcatcagat agatggcatc
     1741 attagtatat ttgctttccg gatatctgat gataaactcg ttcatcaaag tatcagccaa
     1801 agctgtttca ccagctaaaa aagtatttaa aaagttcaag taagatctag tttccatcag
     1861 ggtattataa tttatggttt tcagtatatt atctgctttc tccttttctg aattcaaaat
     1921 atataatcgt gctatttcca gatccatttc ctgagcttcc aaattgttac ggctaaattt
     1981 tttagcttca ttcagccaaa ccaaagcact atctactggt tcatttttag caagcataat
     2041 ttccgccatc agtttgcgta gttttacgct aacattagag cgataataca gatttctatc
     2101 cttccagaaa ggcatcgata aacagccaga aattatttgc tcacttccag gataatctct
     2161 ttgctgatat tttaattcag ccatccgaaa aagcaaatcc atctttttaa cctggtctgt
     2221 ttctatattc tccaaatagc tgtatgcctc aaaagcaata ctgtcattac caaccgctgc
     2281 ttgatcttct gcaaaatgcc acattttttg aggatcaagc tgtttataga tggaaagagc
     2341 tgttttataa tccttcatat ttaccaaagc attagcatag agctctttta atacggaact
     2401 tttactacta tctgcaatag cggaaacaac agatatcagt gaaggttctt cttgcaatat
     2461 agttttcagc tgattattag taaaaaataa gtaaaccgga ttactctctg tataagccat
     2521 atattcacga atagcttttt caaattggcg acagttcaaa gccgtatttg ccatctccaa
     2581 cctgaataaa tcaggtttac ccagacggtt acgagccatt gtatatagct ccataacctt
     2641 gtcataaaat gctttgcgtt caaaaaagga agctaaccgc cgaaatttaa attcatcata
     2701 accaaaattt gccaaatgtc gcattgcttc ctgccaggca tcatctactt tcgcttgtgt
     2761 aacaagtaat tgaatataga attcgctata agtttgcgga ggcaatattc tttggtattt
     2821 ttttaaagtt gtctcagctt tatctgtttg cgaaagagta aaatagaggt tcatcagttg
     2881 gtttattgaa tttaagtcgt tagggtattt atccaggatc tgtaaaaaaa gttgttctgc
     2941 ttgagcatat tgacgctgaa taagcaattg atttgcctgc tgagtaagaa tttgcttttc
     3001 atcatactgt gcatatatag cactctcgca agtaattagt attagtaata gcagagctat
     3061 ctttttcatt ttttatttat catttgctag tagcttcatt cagcgctttt aaatattgca
     3121 taataacttg ttgatattcc ttaggatagg ctcgtaaacc ttcgtccaga agtccactgc
     3181 gtcttaatgc ttctaaatct attttagtat tgcctaagat gggtatgtct tgtccagcct
     3241 cagcttttct cttttctgta aattcgcgtt tattaatgga acgctgggca tcgagcattt
     3301 tactaataat tctctcctgt ctttccagta attcaggatt caactgattg tttttcaatt
     3361 gacgagtgat cgcctccatt tcttctgtaa tttgttttat cacatttccc tgtttttgtg
     3421 cttcaggatt attttgtaaa gctcttttca ggttttcagc aaggcgttct tgatcagcag
     3481 ccaatctttg aatttgttgt tgcatagcac tatccatttt tccgccttga gcctgcaatt
     3541 gtaacattaa ttgttcagta agcatattca tagccatttg ttcttgactc atttgttcca
     3601 gcatttgtaa aagggattgc attcctcctc cacttccgct accagaagag gcattttgca
     3661 atgcctgcat cagatcataa accattaaat taatcccttt ttgaataaaa gtaaggttat
     3721 tgggaatttg atagaactgc gattcattta tcgagctgaa gacattgcga taagcattat
     3781 tagtatcatt taaatctatg aagaattttg gtgggatcat catcagcact tgagtttcgc
     3841 taaacaattt attcagagat atctgaattc cttccgattg agaaatgagg tcatgaataa
     3901 tagcataagg atcatttcga tagcaggcag tggttgcttc atgctgttta gaaaaaatta
     3961 aaagttctct aatcgcggtt tggactgcct gcagcattct ttgttgattt cctccgccca
     4021 ttgattcttt cattttattt agttttaagg aaaagcgtcg catcttttcc agagcgttat
     4081 tttgagctga ttgagattca cttcgctgat tttgattgag ggcattttgg ctattttgaa
     4141 agtcctgttt caggttactg tttttcatat cttgcatcag atcgttaagt tgcttaataa
     4201 catcgttatc acggggtgat tccaacattt cactaatctt gctaagttct tgttgcaatt
     4261 tatcgtaggt gtcgcttatt tgtttttgat cggcagcaag gtctttgggg ttttgctttc
     4321 cgtccatagt cctttcgctt agtgtctttt gcattttttc catttcttcg gaaatctgca
     4381 aggctttttg caaggcttgt tcttttttta tactttccag cagggcaagg gtttggtcta
     4441 ttttcttggc aaaatcctcc atagagaatt tgaaattttc cagtgctttt ttcagatctt
     4501 ccgtttttac attttgcagc gcattttcaa atttacgcat tgcttcttgc agttcttcat
     4561 tattgatttc ctgcatcaat tcctggattt tttgcatttt ttgcagggtt tcaggagaga
     4621 gggtttcatt tgcctgcatt ttattaatca gttcctgata gttatcagca atgttctgaa
     4681 cctgttcaga gaggttctcc tgtttttgta agatctgttc cagctgtttt ttatcttccc
     4741 attgcagatt ttcttttttc agaagttcgc ggcgtttgtt ctcaaattct ttttgcaggt
     4801 ctttcgattt ttccaaagcg gtttgcagtt cgcttttctt ttcgtgttct ttggtttcaa
     4861 tttctctata aatctcttca atggaaggaa atcgcgcttt aaatcttgta ctttctgcct
     4921 tatgttgttc tggagaatta tcatatatag tagcccaata agtaacaaca tcgccaggaa
     4981 ataaattcgc ttccttcaaa tccaaaagaa aatcggttgt aaacattttg ccagaaataa
     5041 cactttgcac attcagcacc tgtggctcag aagccataat ttgaatattg agagcacagt
     5101 tcttcaaacc ataatcgtca tctgcttcaa taattaaagg cagcaacata ttttgattca
     5161 gattaacatc ctcaccggga aaagttattc ttatttctgg aggattatct tccagaatag
     5221 atatggtctt ttcttcaggt ttgttttgcc ttcccaaagc atctgttaat tccaaatacc
     5281 aggtgcgagg tttttctata gaaagttgaa caatgtaatt acttttatct aatgcttgca
     5341 aaggaagccg gctggcatca tcaaaaacca taactgcaga ttcaacagga atatttgtgc
     5401 taaaagaaag tttcacttta ctatatttca aagcctctat attaccgtaa cttaaggtat
     5461 cagtccagga tgttaatccg gaatatgcag gcggattata ttgcacaaac cactttttca
     5521 caatcggttc atccaaaacc tgaattttat atattggcga tttgcatacc tcattttgcg
     5581 catagtattc aatgctgtta tcaagacgct gaaatatata actattatca gctaaaccta
     5641 attcccgcca ttgtttatct atccgataaa atagatggtg ttgcaaacgg ggatcaggat
     5701 tgagaatttt tatctctatt ggctggtttc tgccaattgt tttgtttcca ggggataatt
     5761 ctatatagtc cttgtaaata acttcctggc ccttattcgt gtaaaattgc ttgaaggcaa
     5821 gacgaaaatc tgaaaacgaa tttgcccaaa tactgcctaa acccacaatt agaaaaagaa
     5881 tagcaaaaac tatccaagaa ggatagagcg caggaatttt atattgattc tcttttatgc
     5941 ggtgctctgc taaaatgctc aaattttcca taatcggact atctttctct ttttcagcta
     6001 gttccagggt attttgaaag aggtcatccc tataatcaat ctgtttatcc agaaacctcg
     6061 ctacggaaag attgtgccaa aaattgcggt agccactcca aaggaaatat atgataacca
     6121 ttattaaaca agtccgtatg ccaatattag catacaagag agccatagat tgagaaggca
     6181 aatttaacca taaaagaaaa tatatgtgca aagctaccgc aaagctaagc agggaaccaa
     6241 taattgcccg cagaacaaag cgtaaattaa tcttttgcct atatttctga atgaattttg
     6301 taagctcgct cattttatag catctcccct ttcttgaaag atctttttcc tctaataccg
     6361 ctattatgtc aagaagtttc ctgatgagca catattatga ttgcaacgag caaagaagaa
     6421 ggctgataga caatatgata tttaccagcg cattttttgc acaatatgct aattatctta
     6481 tagccctgcc taatgaacta tggtacagat ggggtgggaa tcttatggga ttttccatag
     6541 ttatgccacg ccagtggcac cataactaac tatgcaatga acaagactaa aagggagata
     6601 ttaaatcccg aacttttaga gtttttctct attctgtaat attttttttg ttgttatgaa
     6661 tgccaccgca agcgcttgtt taatatacta aacaataaac accat
//
LOCUS       DAORHB010000062         6507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_11792_length_6507_cov_4.499380, whole genome shotgun sequence.
ACCESSION   DAORHB010000062 DAORHB010000000
VERSION     DAORHB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11792_length_6507_cov_4.499380"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /locus_tag="PKM80_02970"
     CDS             complement(<1..1965)
                     /locus_tag="PKM80_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNV92899.1"
                     /translation="MKYLHLIILILFTVSIFAITNEEIRFLCDKEQFQLLAKHYDTIQ
                     KKIKTGDEEDLDTVLYYAHRAYLQDIAKQCHKRLAKDYHSLEDALQWLFLCESSEVDS
                     LTLYNDIDSITQALTDPLDKNVWLYYTKNCSEEELFSQVAASEKYNTIIEAMAKDLID
                     EISIEQSDSLALLKLQEFDNRYPNSQYRSIALYYKLYNLANRKNWAGLIESLPPLENL
                     DPPSAYISSLFLLSPTFRRDFTGTEDLLDLSDKYLAKAMSDKEQTLLYDLYLAEDWKA
                     RVLQQQVKLQYYRLVLPYGLYGDEVETPVLDKSNLKQQKELLALQNKVHFTNNNRGEQ
                     AEEHFWMAKVLLLTGKKTDKQKAANQLTQCLILGSPRNRYDVEAMAIIQNLHRDLKIK
                     EEPLQWMQKIAIYKGICFEDLSDKANLNKKGYTRVALADYNSDGLVDILFNGKYLYKN
                     TGNMQFSEVTDSVGLTDLHSNGGIFADFNKDGLLDFVSYSHSSVGRGDQLMKNIDNTS
                     FVNVNERAGDIDDTYPTEAVAWIDINKTGYPSLYAANYEIWQKRAGFPDFFWENKDSF
                     FTDQSYNYGFREPYYSDKPGLPGRGVAPADFDNDGKQEIYVTNYRLSRNFCWKQNDDI
                     FVDVAALYGLDGIYKNGYYGHSIGADWGDYD"
     gene            complement(1968..2843)
                     /gene="hslO"
                     /locus_tag="PKM80_02975"
     CDS             complement(1968..2843)
                     /gene="hslO"
                     /locus_tag="PKM80_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNV92900.1"
                     /translation="MNKDYLIRGTAHHNNFRIFAVQSTNCVQTVRDLHDLSPLATLLL
                     GRMISAAAMLSWDLKDEGSEVTMRIDCEGDIKGAIVICTQDGRLRGYTKKPQLFYNNR
                     EDNFKVGKAIGKGTLTISRDLKGIRSYLSTTELISGEIAEDLAYYFQQSEQIPTAVNL
                     GVLIDKTAKVRAAGGFIIQQLPFADEKTADIINENLNLTPNLSDLMDMGLSIPDVLSR
                     FVFKGIQWQINEEKDIAYHCNCSSELFSKALLLLGKEELQTLKEGIKPVCLYCNKVYD
                     FTAEDIQQLIKQLQE"
     gene            complement(2836..4377)
                     /locus_tag="PKM80_02980"
     CDS             complement(2836..4377)
                     /locus_tag="PKM80_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNV92901.1"
                     /translation="MAKEIPVQLCNDLYTMLKCNCLFILLLLFIPLLWGAQFRDGELY
                     LEINLAASPTLSITSERKDEKITVSEMDGLISRSFDSDVYFFIVNPEKTVRYAILDYT
                     EIISEPLDGKNAVVRNFFAWENSHLKIKTELQHFQAESFNTEEEALQYASLYNIPLDK
                     IKEVQLINTTVQIQDAKGNICFMETPLKIHSDTDLYFNSNSYGYNGDFILKTVKKKIV
                     LNQFLPLENYLAGVVQNEIGNNADMEALKAQAVAARTHALNLLLNNKHKVDGYDLCNT
                     THCQVYKGKYLQDEHILRAVYGCASEVLLINDNLAEATYHSCCGGKTDASSIIWKGKP
                     VDHLNGVICISEAEEYDLTKESDTRRWIDEQLSTEGMSSWERGALSWHKSIPLKQIAD
                     NAGLAYINRIEIHKRGNSGRITNMKLIGNETVTYNNEYQIRYIFGNLLSSFFYIEGNY
                     TENNGITVIIPKASVNVKGRGAGHGVGMCQVGALKKAREGDNYHDILQHYYPTTTINK
                     DWMDE"
     gene            complement(4343..6379)
                     /gene="metG"
                     /locus_tag="PKM80_02985"
     CDS             complement(4343..6379)
                     /gene="metG"
                     /locus_tag="PKM80_02985"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNV92902.1"
                     /translation="MKYLITSALPYANGRLHIGHIAGAYLPADIFVRYLRLKDEDVIY
                     ICGTDEHGTPISITADKEGITPQEVVNRYHQSIKEAFDGIGIEFDNFSGTARPPHYKL
                     ASEFFLQLYNKDFIKPKITLQFYCEHDKRYLPDRYVEGLCPRCGASNARGDQCDKCGQ
                     IYETTTLQEPKCKICGSTPVIKETKHWFLQLDGFRDKLKRWLSTKDYWKENVRNFIFG
                     LLDQGLVERSITRDLSWGVPVPLPEAKGKVLYVWFDAPIGYISSTIEWAEKIGQPEKW
                     KEYWLNPETRLIHFIGKDNIIFHSLIWPAMLMGQDKKYCLPYDIPANEFMNLEGEKIS
                     TSKNWAIWVDEFLQDFEGEYLRYYLAVNAPEKQDSDFCFKDFQSKINTDLNNTLGNLA
                     NRVFAFANKNFEGKITACSLSKSAKLIIDEADAILKEIEECYLDYQVKKNTRLIMDIA
                     RLGNRYFDENKPWAILKENREAVNETLYVCANLLAKISVALFPILPSSMLKLREMMGL
                     SDQFLFNEAYNILDDIKLTDIKPLFHKIEDKEIEAQIAKLHLQTKINEGDKVEFATSK
                     IAPLKELISYDDFSKLDFRLAKVLTAEKIPNTDKLLKLQVDIGIETRELVAGIAADYE
                     PQDLIGKTILMLVNLEPRKIRGINSQGMILAANANGKLLVLNPDGEGNPGSVVQ"
BASE COUNT         1980 a         1263 c         1058 g         2206 t
ORIGIN      
        1 atcataatcg ccccaatctg ctccaatact gtgtccataa tagccatttt tatatattcc
       61 atctaaaccg tataaagctg ctacatccac gaaaatatca tcattttgtt tccagcaaaa
      121 attacggctc aaacgatagt tggttacata aatttcctgt ttgccatcat tatcaaaatc
      181 agcaggagct acacctcttc caggcaagcc aggtttgtct gaataatacg gttcacgaaa
      241 tccataatta tagctttgat cagtaaaaaa gctatcttta ttttcccaga agaaatcagg
      301 aaaacctgcc cgcttttgcc agatttcata attagcagca tagagtgatg gatatccagt
      361 tttgttaata tcaatccaag caacggcttc agttggataa gtatcatcaa tatcaccagc
      421 gcgttcatta acatttacaa agctggtatt atcaatattt ttcattagtt gatcgcctct
      481 cccaaccgag gaatgactgt aggaaacaaa atccagtaat ccatctttgt taaaatctgc
      541 aaagatacct ccattgctgt gtaaatcggt taagcctacg ctatccgtaa cctctgagaa
      601 ttgcatatta ccggtatttt tatatagata ttttccgttg aataggatat ctactaaccc
      661 atcagaatta taatccgcta aggcaacacg agtgtaaccc tttttattta aatttgcctt
      721 atcacttaaa tcttcaaaac agataccttt atagatagct attttttgca tccattgcag
      781 cggttcttct ttaatcttaa ggtcgcgatg caagttttgt ataattgcca ttgcttcaac
      841 atcataacga ttacgaggtg aacctaatat taaacactgg gtaagttgat ttgctgcttt
      901 ttgtttatct gttttcttgc ctgtaagaag taatactttt gccatccaga aatgttcttc
      961 tgcttgttca ccacgattat tattagtaaa atgaacttta ttttggagag caagtaattc
     1021 cttttgctgt ttcaggttgc ttttatccaa aaccggagtt tccacttcat ctccataaag
     1081 accatagggg agaactaaac gataatattg caattttacc tgttgctgta aaaccctggc
     1141 tttccaatcc tctgctaaat ataaatcgta aagcagggtc tgttctttat ctgacattgc
     1201 tttagccaga tatttatcag ataaatctaa gagatcttct gtgcctgtaa agtcccttcg
     1261 aaaagtaggt gatagtaaaa ataaggatga tatataggca gaaggggggt ctaaattttc
     1321 aaggggaggc agggattcaa ttaaaccagc ccaattttta cggttggcaa ggttatatag
     1381 tttataatat agggctatag aacggtattg ggaatttggg tatctgttat cgaattcttg
     1441 cagttttaac agagcaagag aatcactttg ctcgatacta atttcatcaa tcagatcctt
     1501 cgccattgcc tcaataattg tgttatattt ttctgaagca gcaacttgag agaaaagttc
     1561 ctcttcactg caatttttgg tataatataa ccagacattt ttatccagag gatcggttag
     1621 agcttgagtt attgaatcaa tatcgttata tagggtaagg gaatctactt cagatgattc
     1681 acacaaaaaa agccattgca aggcatcttc caaagagtga tagtcttttg ctaaacgctt
     1741 atgacattgt ttagcaatat cttgtagata tgctctatgg gcataataga gaaccgtatc
     1801 aaggtcttct tcatcacccg ttttaatctt tttttgaatt gtatcatagt gtttggcaag
     1861 taactgaaat tgttccttgt cacataaaaa tcttatctct tcattggtta tagcaaaaat
     1921 gctcacagta aacagaataa ggataattaa atgtaagtac ttcattgtta ttcctgtagt
     1981 tgtttaatta gttgttgaat atcttctgca gtaaaatcat aaactttatt gcaatataga
     2041 catacaggtt taatgccttc cttcagggtt tgcaattctt cttttcctaa tagtaataat
     2101 gccttgctga aaagttcaga actgcaatta caatggtagg caatatcttt ttcttcgtta
     2161 atctgccatt gtattccttt aaaaacaaag cgggaaagaa catctgggat ggaaagtccc
     2221 atatccatta aatcgctcaa gttaggtgtt agattcaaat tttcatttat aatgtctgct
     2281 gttttttcgt cagcaaaagg taattgctga ataataaaac cccctgctgc acgtactttg
     2341 gctgttttat cgattagaac acctaagttt acagctgtag gaatctgttc cgattgttga
     2401 aagtaatagg caaggtcttc agcaatttca ccactgataa gttcggttgt gctaagatat
     2461 gatcttatcc ctttcaggtc tctacttata gttaatgtcc ccttaccaat ggctttacct
     2521 actttaaaat tatcttctcg attattataa aagagctgag gcttttttgt gtaacctctt
     2581 aagcgtccat cctgagtgca gataacgatt gcacctttta tatctccttc gcaatctatg
     2641 cgcattgtaa cctcactacc ttcatctttt aaatcccagg aaagcatcgc tgcagcagaa
     2701 atcatccttc ctaaaagtaa agtggcgaga ggagaaagat catgtagatc acggacagtt
     2761 tgaacacagt ttgtactttg cacagcaaaa atccgaaaat tattatgatg tgcagttccg
     2821 cgaataagat aatctttatt catccatcca gtccttattt atagtcgttg taggatagta
     2881 atgctgtaaa atatcatgat agttatctcc ctcacgtgct ttttttaaag ctccaacctg
     2941 acacattcca actccatgtc cagcacctct accttttaca ttaacacttg ctttggggat
     3001 aatgacagta attccattat tctctgtgta attaccttca atataaaaaa acgaagaaag
     3061 gagattacca aatatatacc tgatttgata ctcgttatta taagtaactg tttcattacc
     3121 aattagtttc atatttgtaa tccgtccgga atttcctctt ttatgaatct cgattctatt
     3181 tatataggca aggcctgcat tatcagctat ctgttttaat ggtatacttt tatgccagga
     3241 aagagcaccg cgttcccaag aactcatccc ttccgtagaa agctgctcat caatccatct
     3301 gcgagtatct gattctttag tcagatcata ttcttcagct tcgctaatgc aaattactcc
     3361 atttagatgg tcaaccggtt ttcccttcca aataatactg gaggcatctg tttttcctcc
     3421 acagcaacta tgataagttg cctcagcaag attatcattt attaataaga cctcagaagc
     3481 acaaccataa acggctcgta gaatgtgttc atcttgtaaa tatttaccct tataaacctg
     3541 acaatgagtt gtgttgcaca aatcataacc atctaccttg tgtttattat tcagcagtaa
     3601 atttaaggca tgagttcttg ctgcaactgc ttgtgcctta agtgcttcca tatctgcatt
     3661 gttacctatc tcattttgca ccacaccagc aagatagttt tccagaggca ggaattgatt
     3721 caaaacaatc ttctttttaa cagttttcaa gataaaatcg ccattatagc cgtaactatt
     3781 cgaattaaaa tatagatcag tatcggaatg tattttcaaa ggtgtttcca taaagcagat
     3841 attacccttg gcatcttgaa tctgtactgt agtattaata agctgcactt cttttatttt
     3901 atcaaggggg atattatata aagatgcata ctgaagagct tcttcttctg tgttaaaact
     3961 ctctgcttga aagtgctgca actccgtttt aattttcaaa tgcgaatttt cccaggcaaa
     4021 aaaattccgc actacagcat ttttaccatc caagggttca gatattattt cagtatagtc
     4081 taatatagca taacgaaccg ttttttcagg atttactatg aaaaaataga catctgaatc
     4141 aaagctgcga gaaatgagtc catccatttc agaaactgtt attttttcat ctttcctttc
     4201 agaagtaata cttagagtag gagaagcagc cagattaatc tccaaatata attctccatc
     4261 cctaaattga gctccccaaa gtaggggaat aaaaagtaac agaaggataa ataagcagtt
     4321 acacttcagc atagtgtaca gatcattgca caactgaacc gggatttcct tcgccatcag
     4381 gattaaggac taataatttc ccgttcgcat tggcagctaa aatcatccct tgagagttta
     4441 ttcctcgtat tttacggggt tccaagttta ccaacattaa aatagttttg ccaattaaat
     4501 cttgaggttc ataatcggct gcaataccgg caaccaattc gcgtgtttca attccaatat
     4561 ccacttgtag ttttaataat ttatcggtat tgggaatttt ttctgcagtc aaaacctttg
     4621 ccaagcgaaa atcgagctta gaaaaatcat catagctgat taattccttc aatggagcta
     4681 tcttggaagt ggcaaactct actttatcgc cttcattgat ttttgtttgt aaatgcaatt
     4741 ttgcaatttg tgcctcaatt tccttatctt cgattttgtg aaagaggggc ttaatatctg
     4801 ttaactttat atcatccaat atgttatatg cttcattgaa cagaaattga tcgcttaacc
     4861 ccatcatttc tctaagttta agcatagaac ttggtaaaat gggaaataag gcaacagata
     4921 tttttgccag caaattagcg caaacatata aggtttcgtt tactgcttct ctattttctt
     4981 taaggatagc ccagggttta ttttcatcaa aatagcggtt tcccaagcgt gctatatcca
     5041 ttattaaacg ggtatttttc ttaacctgat agtccagata acattcctct atttctttta
     5101 agatggcatc tgcttcgtcg attatcagtt tagccgattt acttaacgaa caggcagtta
     5161 ttttcccttc aaagttttta ttggcaaagg caaagactct attagccaaa ttgcccagcg
     5221 tattatttag gtctgtattt atcttacttt gaaaatcctt aaaacaaaaa tcggaatctt
     5281 gcttttcagg agcattaact gctagataat atctcagata ttcaccctca aaatcttgca
     5341 gaaattcatc aacccaaatt gcccaatttt tacttgtaga tattttctct ccttccaaat
     5401 tcataaattc gttggcagga atatcataag gcaggcaata ttttttatct tgtcccatca
     5461 acattgcagg ccaaattaaa gaatgaaata tgatgttgtc tttaccaata aaatgaatca
     5521 agcgagtttc aggatttagc caatattctt tccatttttc aggttgacca attttttctg
     5581 cccattcaat tgtagaagaa atatacccaa tgggagcatc gaaccataca tatagaactt
     5641 taccttttgc ctctggaaga ggaacaggaa ctccccaaga tagatctcga gtaatggaac
     5701 gttcaactaa accttgatcc aataatccaa atataaaatt ccgcacattt tctttccagt
     5761 aatctttcgt agaaagccat ctttttaatt tatcgcgaaa accatccagc tgcagaaacc
     5821 agtgttttgt ctctttaatt acaggagttg aaccacaaat cttacattta ggctcttgca
     5881 atgttgttgt ttcgtaaatt tgaccacatt tatcacattg atcacctctg gcattactgg
     5941 ctccgcaacg aggacataat ccttccacat atctatcggg taaatagcgt ttatcgtgtt
     6001 cacaatagaa ttgtagcgta attttgggct tgataaagtc cttattgtat aattgtaaaa
     6061 agaactctga agcaagttta tagtgtggtg gacgcgctgt tccgctaaaa ttatcaaatt
     6121 caataccaat accatcaaat gcctctttaa tgctttgatg ataacggttt actacttctt
     6181 gaggtgtaat tccttcttta tcagcagtta ttgaaatcgg agtgccatgt tcatctgttc
     6241 cacaaatgta aatcacatcc tcatctttta aacgcaagta acggacaaag atgtcagctg
     6301 gtaaataagc tccagcaata tgtccgatat gtaatcttcc attagcatag ggtagagcac
     6361 tggtaattaa gtatttcatt gcttttcctc aatataaatt gtatggagtt tttgaacaac
     6421 ctccgaaaga tggttgttag gcaataaaag ttctatgctc tgttcgttgt tataactgcg
     6481 taaaatatta taattcttgc tgttttc
//
LOCUS       DAORHB010000063         6478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_11872_length_6478_cov_4.339872, whole genome shotgun sequence.
ACCESSION   DAORHB010000063 DAORHB010000000
VERSION     DAORHB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11872_length_6478_cov_4.339872"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PKM80_02990"
     CDS             <1..823
                     /locus_tag="PKM80_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNV92903.1"
                     /translation="EKPENFFQCGIMEQHTCVMGGALSICGIQTFWSDFGVFGIDEVY
                     NNQRLNDINQTNLKTVVTHVGIDVGADGKTHQCIDYISLARNLFGYKMIFPADANQTD
                     RIIRWLINKPGNYLVTMGRSKIPVLKKIDGSPQYDLDYNYIYGKADVLRDGNDGSMWV
                     CGTPVAKAIKAIDILREEGIFLELLYVSSPLHLDIAQLKAAANKGIIFSVEDHSVNGG
                     LGTSIADGLVANSLSAKLIKMGIKQYPMSGESNELYHWAGLDTASLIATVKKNLQ"
     gene            901..1971
                     /locus_tag="PKM80_02995"
     CDS             901..1971
                     /locus_tag="PKM80_02995"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNV92904.1"
                     /translation="MRYFRNDLVDKKPSKINVPIKKRMMCLNESSLNPYSAIRDHFLA
                     KMEEVQLNRYLSSITNDLHLALTSYIDNSLCKENVLIGNGADDILYHIFLAVRENNNS
                     YAVSLAPSYFDYKTFSAMVGLKMQFVDLNEDFSFNTDKYLELANHPDCKLAILCNPNN
                     PTGNLFPDEQLWQIITALPNKLILVDETYYEFSGKTFVGDLSAHPNLILVRSFSKAFA
                     AAGLRFGYAISSEENIYELSKVLTTFHTSILNQTFALSILENKDIFQKQVQQTVFLRD
                     ELYNTLKTIEEINVHPSATNFLTFTLSDRTPDFFHYLLANEIAVRDVGSHPVLHNCLR
                     VTISNREDVEAFVQCVKQFINK"
     gene            2392..3036
                     /locus_tag="PKM80_03000"
     CDS             2392..3036
                     /locus_tag="PKM80_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNV92905.1"
                     /translation="MALILKTTRFIETQIDAFLDIVSDSATTFELAIEDYLNHRIDQF
                     ENRLSQIRENEHRADDLRVSAERFLYERTLIPENRGDVLAILENTDEVIDHIKDSLVQ
                     FSIEMPQIPEVLNDLWIQTTKASTNAVEQLSFAVRSFFRDLAAVNNYIHKVYFFEREA
                     DHIGERLRREIFNLDIELSHKSQLRFFAIQIEQISDYAQAVCDRLSIYVIKRQL"
     gene            3037..4518
                     /locus_tag="PKM80_03005"
     CDS             3037..4518
                     /locus_tag="PKM80_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNV92906.1"
                     /translation="MIFIYLLSGLFLGWSLGANDTGNIFGAAVETRMLKFKTAALIAS
                     IFIFLGAVLEGGGPSGTLGRLGSVDALGGAFTVALAAALTITVIVRTGIPVSTSQTIV
                     GAIIGWNFFSGRLTDFRNLLTIVASWVIAFVLSGIIAAILFYLIKPIINKGKIHLLEQ
                     DVYIRLGLIIIGAFGAFSLGANNIANVVGVFVPVTPFKDINFGSVTLTGVQQLYILGA
                     ISIIVGIYTYSHKVMRTVGKDIFHLSPVTALIAVTAEAIVLFIFASRSLQKILLSIGI
                     PPIPLVPVSSTQVIVGAVVGIGLVKGGKNIRYNILGKVSLAWIAAPVIAFFFSFITLF
                     IVQNVFEQTVYQKTVYMFNKTVINKIETQGLDTNHLSTVNGRKFDREIEVYKELKQAE
                     CFSRNQILSIIQIAEIYPLKVNTHLLQERGLANHFSRQQWEALVKLEGYEFKHKWQLE
                     EKLSKDFFWQKREEVTERDKLYNKKLDEQMNLLYRTFYLPPSQ"
     gene            4515..5417
                     /locus_tag="PKM80_03010"
     CDS             4515..5417
                     /locus_tag="PKM80_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNV92907.1"
                     /translation="MNPFVEKIIPLILAFFIGIAFKFMKLLSQEDAPVLLKVVLNVCL
                     PCLTINSISNIKLQPNMALIPLTAIIVVLLMYIISTGIGKMLKMEKKTFGSFLVGTMI
                     MNTAYALPFFFAAYGDEGLARASLFDIGNTFMIFTFTYYNAIKYGDNNQTTKIQWKKF
                     LRLPPLWALVIAFAWKLFKAPMPNLATNFLNLMGQPTVPLMMIALGLYFDPKLCNLGK
                     AAIAVFIRMGVGLGIGIALSALFGLSGITKSVVIVCGALPIGFNTLIFANLENMDREF
                     AATIVSISIFISLFYIPWLIYIFR"
     gene            complement(5658..>6478)
                     /locus_tag="PKM80_03015"
     CDS             complement(5658..>6478)
                     /locus_tag="PKM80_03015"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNV92908.1"
                     /translation="KTDGLKGADIYFTKSSVGATINTLMAAVLAEGKTRLFNAAMEPE
                     VDSTIELLNKMGAKIDGIGTTTLEIEGVEELLPAEMDMIPDRIEAGTFLIAGALSKNP
                     VTVANCEPSHLTAVLEKLKEAGCELEIEESTITVIPPVSIKPVNLMTLPYPGFPTDLQ
                     AQFTVLMTLAEGVSFIEDTIFPERYMHIAELNRLQANIKIDRNIAAVRGVKELSGAEV
                     MATDLRASAALVLAGMVANGTTIVSRIYHIDRGYECIEKKLNSIGANIIREEVS"
BASE COUNT         1919 a         1143 c         1207 g         2209 t
ORIGIN      
        1 agaaaaacca gagaattttt tccaatgtgg aattatggaa caacatacct gtgtgatggg
       61 aggagccctt tccatttgtg gaattcagac cttctggagt gattttggag tttttggcat
      121 tgatgaagtt tataacaatc aacgcttaaa tgatattaac caaactaatc tgaaaactgt
      181 ggttacccat gtaggtatag atgttggagc agacgggaaa acacatcagt gcattgatta
      241 tatctctttg gcacgcaatc tgtttggtta taaaatgatc tttccggcag atgctaatca
      301 gaccgataga attatccggt ggttgatcaa taaaccagga aactatctgg ttacaatggg
      361 ccgttcaaaa attccggtgc tgaaaaaaat tgatggatcg ccgcaatatg atttggatta
      421 taactatatt tacggaaaag cagatgtcct tagagatgga aatgacggca gtatgtgggt
      481 ttgtggaacc cctgtggcca aagcaataaa ggcaattgat atcttacgcg aagaaggtat
      541 ctttctagaa ttgttatatg tttcttcccc cttacattta gatatagctc aattaaaagc
      601 tgctgccaac aaaggaatta tcttcagcgt ggaagatcat tctgttaatg gtggtttggg
      661 aacttctatt gccgatggtt tggtagctaa ttctcttagc gcaaaactta taaaaatggg
      721 tattaagcaa tatccgatgt ccggagaatc taacgaatta tatcattggg caggcttgga
      781 tactgcttca ttgatagcta ccgtaaagaa aaatctacag taaagcgaat acaatttttt
      841 cagctttact tttccctgtt ttcagcttat aataaataaa tgaatatcag gagtaaacaa
      901 atgcgatatt ttcgtaacga tctagtggac aaaaaaccct ctaaaatcaa tgttcccatt
      961 aaaaaaagaa tgatgtgctt gaatgagagt agtctaaatc cctattcggc aataagagat
     1021 cattttttag ctaaaatgga agaagtgcag ctgaatcgct atctttcctc aataacgaat
     1081 gatttacatt tagcgctaac atcatacatt gataattcgt tatgcaaaga aaatgtccta
     1141 attggaaatg gcgctgatga tattctttac catatctttt tggcagtgcg cgaaaacaac
     1201 aattcctatg ctgtttcttt ggctccttcc tattttgatt ataagacctt tagtgcgatg
     1261 gttggtttga aaatgcaatt tgtggactta aatgaggatt tcagttttaa tacggacaaa
     1321 tatttggagc ttgccaatca tcctgattgt aaattagcaa ttctatgcaa tcctaataat
     1381 cctacgggca atttatttcc tgatgaacaa ttatggcaaa taattacagc tttaccaaat
     1441 aaactgattt tagtagatga aacttattat gaattttctg ggaaaacctt tgtaggagat
     1501 ctttccgctc atcccaattt aattcttgtg cgttcttttt ctaaagcatt tgcagcggca
     1561 gggcttcgct ttggttatgc tatatcttca gaagaaaata tatacgagtt atccaaagtg
     1621 cttaccactt ttcacacttc catcttgaat cagacatttg ctctctctat tctggaaaat
     1681 aaggacattt ttcaaaagca ggtacaacag acagtttttt tacgagatga gctttataac
     1741 actttgaaaa ctatagagga aataaatgta catccatccg ctaccaattt tctcactttc
     1801 acgctatctg ataggacacc ggattttttc cattatctat tggctaatga aatagctgtt
     1861 cgtgatgtag gttcgcatcc ggttttgcat aattgcttaa gggtaacaat cagtaatagg
     1921 gaagatgttg aagcatttgt gcaatgtgta aaacagttta taaataagta agtaagaagt
     1981 catctgcagt ttctattttt tgtctcctat gcttatttaa cattcgcata tggcttatgt
     2041 tctttatcgt tatatctgct tacatcaggc attcatccag accccaatat ggctttttta
     2101 ttgtttatat gaacttaagc atcaagagca gaattacata ttttggcatt gcaaccaatt
     2161 aaatttgaga aagaatattt tatctgcggg taacaattaa ctctttacct taaatcctct
     2221 gcaaaaaaat cctgctcatc tcagacctat tctattattc gttacaggca ttaatctatt
     2281 gtaatccaca aaatctgtgt cctatacctt ttttcctgct tgacagaaag tacaatctat
     2341 ctttctggta aattatatat aataacataa aatatgctta agggagagag aatggcgcta
     2401 atcttaaaaa cgactcgttt cattgagacc cagatcgatg cttttttaga tattgtaagc
     2461 gattcagcaa ctacttttga actggcaatt gaagattatc tgaatcatcg gatcgatcag
     2521 ttcgaaaacc gcttatcgca aattagggaa aatgaacatc gggcagatga tttgagggtg
     2581 agtgctgaac gctttcttta tgaacgtacc ttgattcctg aaaacagggg agatgttctt
     2641 gccatattag aaaataccga tgaagttatt gaccatataa aagactcttt agttcaattc
     2701 tctattgaaa tgcctcaaat acctgaagtg ctaaatgacc tatggataca aacaaccaag
     2761 gcatcgacca atgccgttga acaactttct tttgccgttc gttcattttt tagggattta
     2821 gctgccgtga ataattatat tcacaaggta tatttctttg agcgggaagc agatcatatc
     2881 ggagaacgcc ttcgtcgtga aatatttaat ctggatattg agctttccca taaaagccag
     2941 ctacgttttt ttgctattca gattgagcaa atttcagatt atgcccaagc agtatgtgat
     3001 cgtctatcta tttatgtaat caaaagacag ctttagatga tttttattta tctgcttagt
     3061 ggtctttttt taggttggtc tttaggagcc aacgatactg gcaatatatt tggtgctgct
     3121 gtagaaacca gaatgttgaa atttaaaaca gctgccctaa ttgcctccat ttttatcttt
     3181 ctaggtgctg tcctagaagg gggaggacca tctggcactt taggtcgttt aggaagtgtg
     3241 gatgctttag gaggggcatt tactgttgct ttagctgctg ctttaacaat tactgtcatt
     3301 gtcagaacag gaattccggt atccacatct caaactattg taggtgcgat catcggttgg
     3361 aacttctttt ctggacggct tacagatttc cgtaatttgc taactattgt tgccagttgg
     3421 gtaattgctt ttgtactttc tggaattata gctgcaatcc ttttttactt gattaagcct
     3481 attatcaaca agggtaagat tcatcttttg gagcaggatg tttatatccg tttggggctg
     3541 ataataattg gtgcctttgg tgctttttct ttgggtgcca ataatatagc taatgtagtt
     3601 ggggtttttg ttcctgttac tccttttaag gatatcaatt ttggcagtgt tactttaacg
     3661 ggggtgcagc aactttatat tttgggcgcc atttctataa ttgtgggtat ttatacatat
     3721 tctcataaag ttatgcgtac agtgggaaag gacatttttc atctttcacc tgtgactgcg
     3781 cttatagctg taacagctga ggcaattgtt ctttttattt ttgcttcacg ctcgctgcaa
     3841 aaaatactgt taagtattgg tataccacca attccacttg ttccagtatc ttccacacaa
     3901 gtaattgtag gagcggtagt gggcataggt ttagtaaagg gtgggaaaaa tatcagatac
     3961 aatatattgg gcaaggtctc attagcttgg attgctgctc cggtgattgc ttttttcttt
     4021 tcttttatta ctttgtttat tgttcaaaat gtctttgaac aaactgttta tcagaaaaca
     4081 gtatatatgt ttaacaaaac agtgatcaac aagatagaaa cacagggatt agataccaat
     4141 catctttcta cagtaaatgg acgcaaattt gatagagaaa tagaggttta taaagagttg
     4201 aaacaagcgg aatgttttag ccgaaaccag attctgagca tcatccaaat agctgaaata
     4261 tatcctttaa aagtaaatac ccatctatta caagaaagag gtttagccaa tcatttcagt
     4321 agacagcaat gggaagcgct ggttaaattg gagggatatg aattcaagca taaatggcag
     4381 cttgaagaaa agctgtctaa agattttttc tggcaaaaaa gagaagaagt taccgaaaga
     4441 gataagctct ataataaaaa gctggatgaa caaatgaact tgctatatcg tacattttat
     4501 cttcctcctt cgcaatgaat ccttttgttg aaaagataat tcctcttatt ctggccttct
     4561 ttataggcat cgctttcaaa tttatgaagc ttctttccca agaagatgct ccagttcttt
     4621 tgaaagtagt tttaaatgta tgtttgcctt gtttaacgat aaactccatt tccaacatta
     4681 agttacaacc caatatggct ttgattcctt taacagcaat aattgttgtg ctgctgatgt
     4741 atattatctc cacaggtatt gggaaaatgc tgaagatgga aaaaaaaacc tttggtagct
     4801 ttttggtagg cacaatgatt atgaataccg cttatgcttt accctttttc tttgctgctt
     4861 atggcgatga aggactagcc cgagcatcat tgtttgatat tggcaatacc tttatgatct
     4921 tcacttttac ctattataat gctatcaaat acggagataa taatcagacc accaaaattc
     4981 aatggaaaaa atttttgcgt cttcctcctc tttgggctct ggtgattgcc tttgcctgga
     5041 agttattcaa agcaccaatg ccaaatttag ctacgaattt tctcaatctt atgggccaac
     5101 ccactgttcc tttaatgatg attgccttgg gtttatattt tgatcctaaa ctttgtaacc
     5161 tgggtaaagc agcaattgct gtctttatta gaatgggtgt gggattggga attggaattg
     5221 ccctaagcgc gctatttggt ctaagcggta taacaaaatc tgtagttatt gtttgtggtg
     5281 cattgcctat tggtttcaat accctcattt ttgctaatct ggaaaatatg gatagagaat
     5341 ttgcggcgac catagtctcc atttctatct ttatttccct tttctatatt ccttggctga
     5401 tttatatttt ccggtaattc tctgtatctg gagctacata tttatttgta aggaagatgc
     5461 caccttccga aaataatatt ctggatgaag gatttctata ctaccccagt aaatgttgat
     5521 atattgatat ataaagctac cgctaattgc taatataaat aatcattaga gaattatgga
     5581 ataacttttt tcggattcag gataaagtta ctctgcttta attcgaattt tactttgatg
     5641 tttttagcag taggcgatta tgatacttct tctctaatta tattagcgcc gatcgaattt
     5701 agcttttttt ctatacactc atagccgcga tctatatgat aaattctgga aacaatagtt
     5761 gttccattag caaccatacc tgctaaaaca agagccgcac tggctcttag atctgtagcc
     5821 ataacttctg caccgctaag ttcttttact cctcggacag cagcaatatt tctatcaatt
     5881 ttgatatttg cctgcagacg gtttaattct gcgatgtgca tatagcgttc tggaaagata
     5941 gtatcttcaa taaaactaac tccttcagcc agggtcatta gaactgtaaa ttgagcttgc
     6001 aaatcagttg gaaaaccagg atagggaagg gtcatcagat ttaccggttt gatagaaaca
     6061 ggtggaataa ctgttatggt actttcttca atttccaatt cacaacctgc ttctttcaat
     6121 ttttccagaa cagctgtgag atgtgaaggt tcacaattag ctacagttac cggattttta
     6181 ctaagtgccc ctgctattaa aaaagttcca gcttcaattc tatcagggat catatccatt
     6241 tctgcaggta aaagttcttc cacaccttca atttcgagag tagttgttcc tataccgtca
     6301 atttttgccc ccattttatt caataactcg atagtggaat ccacttctgg ttccatagcg
     6361 gcattgaata atcttgtttt accttcagct aaaacagctg ccattaatgt attgatagta
     6421 gctcctacgc ttgattttgt aaaataaata tctgcaccct tcaacccatc ggttttgg
//
LOCUS       DAORHB010000064         6458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_11929_length_6458_cov_4.074340, whole genome shotgun sequence.
ACCESSION   DAORHB010000064 DAORHB010000000
VERSION     DAORHB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11929_length_6458_cov_4.074340"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKM80_03020"
     CDS             complement(<1..1040)
                     /locus_tag="PKM80_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNV92909.1"
                     /translation="MPYLPINKSDLKQRGWNSLDIILISGDAYIDHPSFAAAIIGRTL
                     EAAGFKVGIIAQPDWKNDSSFQVLGKPNLFFGITAGNMDSMVNHYTAQRKLRNDDAYT
                     PNGEIGKRPDRATIIYTNILKRLFKGVPIVIGGIEASLRRIAHYDFWQDKVRKSILAD
                     SKADILIYGMGEKPITELAMLLKKGKTIDEIQNLTSSVVFSATNPSPEDVVLPDAEIC
                     TDKNTFYQMTKIFEKNYETKVIYQKTAGRWLKHNLPAKPLTTEEMDAIYALPFEYKAH
                     PIYDGHKIPAYEQIKDSLTSHRGCYGGCNFCAIACHQGRRIQSRSENSLLKEAQKHKG
                     TISDVGGPTANM"
     gene            complement(1104..1316)
                     /locus_tag="PKM80_03025"
     CDS             complement(1104..1316)
                     /locus_tag="PKM80_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNV92910.1"
                     /translation="MKGKVKWFNKNKGYGFIITEDNKEYFVHWKSIVTNSPRELKVLE
                     QDELVTFDLMETDKGIQAINIIRVNP"
     gene            1736..2092
                     /locus_tag="PKM80_03030"
     CDS             1736..2092
                     /locus_tag="PKM80_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV92911.1"
                     /translation="MRPRILLVDDDRLLREVIGEYLSNHGYEVDLAEDADYALEKFEP
                     GKYQMAIIDYMMPGMDGLELMKILLERDPKIFCLIMTGYPTVDSAYQSIIDGASDYII
                     KPFHLKELLSTIGSYI"
     gene            2092..2997
                     /locus_tag="PKM80_03035"
     CDS             2092..2997
                     /locus_tag="PKM80_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92912.1"
                     /translation="MIEIELTQPSFLCKSSLNVWNNPDFMQTVADISDLKPYHLVCYK
                     GESVVSCLPLYEKKVFGRKICKHPLLSYYQGLNLWLEDNSAPARRLLDALQITQTIAK
                     YLSCRYKVIRHILSPETYDVRGFLWNNMQAIPSYTFIHNFQNSIIPLSDERKKITLAE
                     KQDYTFEEEMNIEEFIHLMKLMNVKKRWQPGLSYQDLADFIRILHTAGILRQLNLKKD
                     GRIVSSNIILQDCAKAYTIFRATEIEALKKGASSLHTVKLIEFLKQENLEELDFCGAN
                     VSDIARFKAAMDLQLKVFFQIRTTL"
     gene            3044..3994
                     /locus_tag="PKM80_03040"
     CDS             3044..3994
                     /locus_tag="PKM80_03040"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNV92913.1"
                     /translation="MEKIVVLKGGTSPEREISLVSGSEVATALRTMGYIVSEIDPANY
                     PVLSNFITAIQNEHPLLVFNGLHGGSGENGELQATLSMAGIKYTGSNFKASCISMDKY
                     ISKLIAIAEGIPVPRYILLREDLLVDYQDPEDYSGFSNQLGLPIIVKPNDAGSSVGIS
                     RVEYLEDLKSAVQNAFKYSPNVLLEEYIPGRELTVTIIDGEAYPVVEIKPLEGWYDYT
                     NKYTSGKTHYEAPAQIDDTVAKLLQLYAIRLWKAFGLSGYARIDFRYDDLKPYFLEVN
                     TLPGMTSLSLTPMAAKAAGMSFQDLLKNIIDITIKYGGLK"
     gene            3991..5106
                     /locus_tag="PKM80_03045"
     CDS             3991..5106
                     /locus_tag="PKM80_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92914.1"
                     /translation="MKRLIYIIVIIALLPVFANAQANFEVNFVPSSIRINSLSTASFD
                     PTQPDLQPILTTLMIKNLNTTPCRIDLKVALYWNNLLLANTIFCSREDFTLGYCTLNN
                     RDLITDEPMLNFEFKSGSTSISVEDILDASSTLKSAVMAGYFPDGELKFKVWVREFSE
                     TPWEGADNSPSAEFKIIIRNAGNITLLAPGRPIGQNPDKVSGLPISFLWNSQITGFND
                     YKLIIREYAPNNPPTINNVERTGSLFYETPSGKNECSGFSDFLPFTDSNYYAWKVTTA
                     LANEFNPIARGNKNNSLSSNWYIFQYVDEQEAAHSISEFQAHLDLLQNPTLINIHTQG
                     YLPVGVVVIEGKTYSGQEAINLIDALLGQDISVELKK"
     gene            5121..5795
                     /locus_tag="PKM80_03050"
     CDS             5121..5795
                     /locus_tag="PKM80_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV92915.1"
                     /translation="MKRLFLISLALILAINISAANSIAMISASKGKVNLRRAAKVVPH
                     KTGDFLYNKDEIRTGGESFAAYKYIDGSSTVKIFSNSIVYITATKNEKILDKKIELDK
                     GSVFTNIKPNTGSFSVATPTTVASVKGTAFFTKFDEFGQSTFIITEGEVELKILNKDT
                     QSITAGNTAVVQANGSFQIHNSTQEELQEIEQAELESTQGKENKKMRIPVIDPNGQTK
                     YIEITW"
     gene            5806..6207
                     /locus_tag="PKM80_03055"
     CDS             5806..6207
                     /locus_tag="PKM80_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92916.1"
                     /translation="MFKHYLLLMLSMVLTVQIFAISLVHICPQTYTFNQKTDIKVEIQ
                     QGLSDIDKIKVIYRIDKQDHWFNEEMKQDSPGSVYFTATFPAKYLTTNVIEYYFVVEL
                     RQGNKEYFPPQNETVPNYILEPDIAYGEADP"
BASE COUNT         2118 a         1080 c         1153 g         2107 t
ORIGIN      
        1 tacatattgg cagtagggcc ccccacatcg gaaattgtgc ctttatgttt ttgtgcttcc
       61 ttaagaagtg aattttctga tctggattga attctgcgtc cctgatggca agcaatggca
      121 caaaaattac aaccaccata acaaccgcga tgagatgtta gggaatcctt tatttgttca
      181 tatgcgggaa ttttatgccc atcataaata ggatgcgctt tatattcaaa gggtaaagca
      241 taaatagcat ccatttcttc tgtagttagg ggttttgcag gaagattatg tttcaaccat
      301 ctgccagctg ttttttggta aataaccttt gtttcgtagt tcttttcaaa gatcttggtc
      361 atttgataaa aagtattttt atcagtacat atttcggcat ctggtaatac aacatcttca
      421 ggagatggat ttgtagcaga aaaaacaaca ctggaagtta aattttgtat ttcatctatt
      481 gttttgcctt tctttaataa catagccaat tcagttattg gtttctcccc cattccgtaa
      541 attaaaatat ctgctttact gtctgccaga atacttttcc gaactttatc ttgccaaaaa
      601 tcgtaatgtg caattctacg caaggatgct tcaatacctc caattacaat tggcacacct
      661 ttaaaaagcc gctttaaaat attggtatat ataatagttg ctcggtcagg acgttttccg
      721 atctctccat ttggagtgta ggcatcatca tttcgcaatt ttctttgtgc agtataatga
      781 tttaccatag aatccatatt acctgcagtg ataccaaaaa atagatttgg cttccctaaa
      841 acttgaaaac tcgaatcatt tttccaatct ggttgagcaa ttatccctac tttaaaacct
      901 gccgcttcta aagtacggcc aataattgct gcagcaaaac tggggtggtc aatataagca
      961 tctccactaa ttaggatgat atctaaagaa ttccaacctc gttgtttcag gtctgattta
     1021 tttatgggaa gataaggcat tagtaaaaaa aaagggtaag ggctgttttg ccattaccct
     1081 tgaaaaagtt aggtaattaa ctcttaggga ttaaccctaa tgatgttaat tgcttgaata
     1141 cctttatcag tttccattag atcaaaagta accaattcat cttgttctag taccttcaat
     1201 tctcttggtg aattagtaac tatagatttc cagtggacaa agtattcctt gttgtcttca
     1261 gtaataataa agccataacc tttattcttg ttaaaccatt taacttttcc tttcatactc
     1321 acctcacgat ttataatcca attccttaaa atataaatgc ctaaatatgt caactataaa
     1381 aaagcaaaat agcaagatag tgatgttatt atggattaag attgctaatt gcaaaatggg
     1441 aaaagctcgc aggattgagt tatttttcac aataggtttg aatatagcat aaatatagaa
     1501 gtttgggaag agttaatcgg tttttgtttt ttcccaggga taattaactt tattagctct
     1561 tttctttcta tgccttaaca caattgaata acattattca tcctatactc ttttcgtccg
     1621 ttaagagtac aatctttatg gatagatcca accttttttc atttttatat tgactatttt
     1681 aaggatttga ttatatttga tgttatgggt taatattata tagataggag tttttatgag
     1741 acctcgaatt ctattggtgg atgatgatag attactacgc gaagtaattg gagaatattt
     1801 atcgaaccat ggatatgagg tcgacttagc tgaggatgca gattatgctt tggagaaatt
     1861 tgaaccgggt aaatatcaaa tggctattat agattatatg atgccaggga tggatggatt
     1921 ggagttaatg aaaatattgc tggaaagaga tcccaagata ttttgtttga ttatgaccgg
     1981 ataccctact gtagattctg cttaccaatc cataatagat ggagcatccg attatattat
     2041 caaacctttt catctaaaag aattattaag cactattggt tcttacatct aatgattgaa
     2101 attgaattaa cccaaccttc tttcttgtgc aagagttctt tgaatgtttg gaataatcca
     2161 gattttatgc aaacagtagc tgatatttct gatctaaaac cttatcatct tgtatgttac
     2221 aaaggagaaa gtgtagtttc ttgcctacct ctttatgaaa agaaagtgtt tggtcgtaaa
     2281 atatgtaaac atcctctttt atcttattat caggggttaa atctttggct ggaagataat
     2341 tctgctccag caagaagatt attggatgct ttacaaatta ctcagacaat tgccaaatat
     2401 ctttcctgtc gttataaagt aattcgccac atattatccc ctgaaactta tgatgttaga
     2461 ggttttcttt ggaataatat gcaagctatt ccatcctaca cttttattca taatttccag
     2521 aatagtataa tccctctttc tgatgaacgt aagaaaatta cactggcgga aaaacaagat
     2581 tataccttcg aagaagaaat gaatatagaa gaatttattc atctaatgaa acttatgaat
     2641 gtaaaaaaaa gatggcagcc aggtttaagc taccaagacc tagcagattt tatccgcatt
     2701 ctgcatactg ctggaatttt acgacaacta aatttaaaaa aagatggtag aatcgtcagc
     2761 agcaatatta tcttacaaga ctgcgctaag gcctatacta ttttccgcgc taccgagatt
     2821 gaagctttaa aaaaaggtgc aagcagctta cacacagtaa aattgatcga atttctaaaa
     2881 caggaaaatc tggaagaact ggatttttgt ggagctaatg tttctgatat tgctagattc
     2941 aaagcggcta tggatttaca attaaaggta ttttttcaaa ttcgtactac attataggat
     3001 ataatatgga ttactctatt aatttaaata gttatgaggt aagatggaaa agattgttgt
     3061 tttaaaagga ggtacttctc cagaaagaga aatatcctta gttagcggtt cggaagttgc
     3121 tacagcttta agaactatgg gatatattgt ttcagaaata gaccctgcga attatccagt
     3181 tttatctaat tttataactg ctatccaaaa tgaacatcct ttgcttgtct ttaatggttt
     3241 acatggaggc agtggagaaa atggcgaatt acaagcaacg ctatctatgg ccggtataaa
     3301 gtataccggt tcaaatttca aagccagttg tatcagtatg gataaataca tttccaagct
     3361 gattgctata gcagaaggaa ttccagtccc cagatacatt ttgttgcgag aagaccttct
     3421 ggtggattat caagaccctg aagattattc gggattttcc aatcaattag gacttccaat
     3481 tattgtaaaa cctaatgatg ctggttcttc ggtaggaata agtagagtgg aatatttgga
     3541 agatttaaaa tctgcagtac aaaacgcttt taagtattct cctaatgttt tattggaaga
     3601 atatatccct ggtagggaat taaccgtaac gattattgat ggagaagcat atcctgttgt
     3661 cgaaatcaaa ccccttgagg gctggtatga ttacaccaat aaatacacat cagggaaaac
     3721 ccattacgaa gcacctgccc aaattgatga tacagttgct aagcttctac aactttacgc
     3781 aatcaggcta tggaaggctt ttgggttaag cggttatgcc agaattgatt tccgctatga
     3841 tgacctgaaa ccttatttct tagaagtaaa cacacttcct ggaatgacct ctctatcgtt
     3901 aactccgatg gcagctaagg cagctggaat gtctttccag gatttattaa agaatataat
     3961 agatattact atcaagtatg gaggtttaaa atgaaacgct tgatatatat tatagtgatt
     4021 attgcgttgc taccggtttt tgctaacgca caagctaatt ttgaagttaa tttcgttcca
     4081 agttcaatta gaataaacag cttgagcact gctagttttg atccaacaca acctgatttg
     4141 caaccaattt taactacttt aatgattaaa aatctgaaca caactccctg cagaatagac
     4201 ctgaaagttg ctctttattg gaataatctt cttttagcaa atactatttt ctgttccagg
     4261 gaggatttta ctttgggtta ttgcacactt aataatcggg atttgattac tgatgaacca
     4321 atgctcaatt tcgaattcaa aagtggcagt acaagtattt cagtagaaga tatattagat
     4381 gccagctcaa ctttgaaatc tgctgttatg gcaggatatt ttcccgatgg agaactcaaa
     4441 tttaaggttt gggttcgaga attttctgaa actccttggg aaggagcaga taattcaccc
     4501 tctgcagaat ttaaaataat tatccgcaat gccggaaata ttactttact tgcacctgga
     4561 agaccaatag gacaaaatcc tgataaagtt agtggattac cgatcagctt tttatggaat
     4621 tctcagataa ccggatttaa tgattataag ctaataattc gtgaatatgc acctaataat
     4681 cctccaacaa ttaacaatgt ggaaagaaca ggatcgctat tttatgaaac ccctagtggg
     4741 aaaaatgagt gcagcggctt ttcggacttt ttgcctttta ccgatagcaa ttattatgcc
     4801 tggaaagtta ctactgccct tgccaatgag tttaatccaa tagcaagagg aaacaagaat
     4861 aattccctat ccagtaattg gtacattttc caatatgtgg atgaacagga agcagcccat
     4921 agtatttcag aatttcaagc gcatcttgat ctgctgcaaa atccaacctt aattaatatc
     4981 cacacccaag ggtatcttcc tgtaggagtt gttgttatag agggcaaaac ttacagtggt
     5041 caggaagcta ttaatttgat tgatgcttta ctgggacaag atatctccgt tgagctaaag
     5101 aaataatgag ggaggtaaaa atgaaacggt tatttttaat ttccttggca ctaatattag
     5161 caattaacat atctgctgcc aattcaatag ctatgatctc tgcctcaaaa ggtaaagtca
     5221 atttaagaag agccgcaaaa gtggttcctc ataaaacagg tgatttttta tataacaaag
     5281 atgagatccg tacaggcggt gaatcttttg ctgcatataa atatattgat ggttcctcta
     5341 cagtaaaaat attctctaat tctatcgtct atattactgc tacaaaaaac gaaaagatct
     5401 tagataaaaa aattgaatta gataaaggaa gtgtatttac gaatataaaa cccaatacag
     5461 gttctttttc tgttgctact cctactacag ttgcctcggt taaaggaact gcctttttta
     5521 ccaaattcga tgaattcggc caatctacat ttatcattac tgaaggtgaa gtagagctta
     5581 aaatattgaa taaggatacg caatctatta cagctggaaa tactgctgta gttcaagcta
     5641 atggtagttt ccaaattcat aatagcactc aagaagaact tcaggaaatt gaacaagcgg
     5701 agctggaatc aactcagggt aaagaaaata aaaaaatgcg tattcctgta attgacccga
     5761 atggacagac taagtatata gaaattactt ggtaggagaa taaagatgtt taaacattat
     5821 ttgcttctta tgttatcgat ggttcttact gttcaaatat ttgctatatc tttagtccac
     5881 atttgtcccc aaacctatac atttaaccaa aagaccgata taaaagtaga aatacagcaa
     5941 ggtttaagcg atatagataa aataaaggtt atttacagaa ttgataaaca agatcattgg
     6001 tttaatgaag aaatgaaaca ggatagcccc ggttctgtat acttcactgc tacttttcca
     6061 gctaaatatt taactaccaa tgtgatagaa tattacttcg tagtagaatt gagacagggc
     6121 aataaggaat actttccacc tcagaatgaa actgttccaa actacatatt agaaccggat
     6181 attgcttatg gagaagcaga tccttgattt gtccttctaa cggataatcc aattgttgct
     6241 gcagaagatg gttatctatt agttgtttcc ttttttgctt tagcggatga aattgatccc
     6301 aaaacaattg aggtttgggt tggaggtaaa aatgtaactg ctaattctca aattatacct
     6361 cctacgattg tatataaaga agaaaaacca gccagcggaa ttgtaagagc tttggttcga
     6421 gctaagaaag aaaaaaatat cgttcactca caaatttg
//
LOCUS       DAORHB010000065         6429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_12015_length_6429_cov_4.141042, whole genome shotgun sequence.
ACCESSION   DAORHB010000065 DAORHB010000000
VERSION     DAORHB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12015_length_6429_cov_4.141042"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PKM80_03060"
     CDS             complement(<1..1342)
                     /locus_tag="PKM80_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNV92917.1"
                     /translation="MKQSKCLTLLLVTIFLLPLAIFAKTGSDKNAFSIINSSSSEIKI
                     QFSLPEWQINQIDDKGEIRKQIKVQDTPYLFIDEEETLPVFSTMIAIPNRGGVNLLVS
                     NSSKSTINEFTANFNDALMRESQQGRYSDVLYPANNVVISEPQILRDFRVITLNIYPF
                     QYNRNNRTLFISENLDITLNFDNRTAGNELNSDIDVSCSFDSIYKGLILNYDSVRQTR
                     NVSYRNPVMLVIYGNNADAIYQAKINEYVTWKKQKGFIVNAVSTSVTGTSNTSVKSYI
                     QNAYNNASTRPDYITLIGDTDGNIAVPSNSSYVDYQYTWLAGNDNLGDVMIGRISVNS
                     TSDLDVILAKIISLEKNISQHPIDWLNRMVLVGDSSSSGISTIYTNRYIRDISEEINP
                     AYTYTEVYGSSPSNTTTNSAINQGVAFYNFRGYIGMNSWPASMSSLYNSYKLFHS"
     gene            complement(1945..2889)
                     /gene="arcC"
                     /locus_tag="PKM80_03065"
     CDS             complement(1945..2889)
                     /gene="arcC"
                     /locus_tag="PKM80_03065"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNV92918.1"
                     /translation="MQKKAVLALGGNAIIKAGEKGTIAQQFSNTRESLGGIVELIKRG
                     YHLSITHGNGPQVGNLLRQQELGEMEGIPQLPLGVLNAATEGTMGYMIEQSLQNKLHK
                     SNINKQVITIISQVVVDKNDPSMLNPTKFVGSTYYTAEQAEEIKNKLGWTLKEDSGKG
                     FRRVVPSPYPIEIIPAATIKELVDKGEIVIAVGGGGIPIYKEDDGSYEGVDAVIDKDF
                     ASSLLALEIKADLFVILTGVEKVSLNYGKENQRNLDKLTVEEAKRYYAEKQFPAGSMG
                     PKIKAAIDFLERGGSEVLITSIDKIVDAFEGKTGTRIV"
     gene            complement(3035..4369)
                     /locus_tag="PKM80_03070"
     CDS             complement(3035..4369)
                     /locus_tag="PKM80_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNV92919.1"
                     /translation="MKRKVIIMGAAGRDFHNFNVFFRDNKDYEVVAFTATQIPGIDDK
                     KYPAALAGKLYPDGINIYPESEIKNLIKQHNVDLVVLAYSDLPYEVVMHKASIINSTG
                     ADFMLMGKDNTTLKTSKPLVTVCAVRTGCGKSQTTRTVVKALKAKGLKVVSIRHPMPY
                     GDLVKQKVQRFAELDDLKKHNCTIEEMEEYEPHIMMGSVIYAGVDYAAILKEAEKEAD
                     VIVWDGGNNDIPFYTSDNEIKIVVVDPHRPGDEISYYPGETNVYLADVIVINKIDSAN
                     LYDINEVRENVRAINPTAQIIEAASPLFVDHPEMILGKKVLVVEDGPTLTHGEMQYGA
                     GVVAAEKYGCADLVDPRPWAVGEIKQTFEKYPGVGSLLPAMGYSSKQIKDLEKTINST
                     ECDSVVIATPIDLRRIIKINKPACQVSYELQEIGVPTIADILKNFGKKSTKK"
     gene            4545..4632
                     /locus_tag="PKM80_03075"
     tRNA            4545..4632
                     /locus_tag="PKM80_03075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4579..4581,aa:Leu,seq:cag)
     gene            4877..6217
                     /locus_tag="PKM80_03080"
     CDS             4877..6217
                     /locus_tag="PKM80_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNV92920.1"
                     /translation="MEKKIFVLDTNVLIHNPQALFSFQDNRVVLPIVVIEEIDQFKKG
                     VDEKSRNARQIGRFLDALRKKGSLQEGVPTENGGTIQITINKEITATASELLFMDRND
                     NLIISTALYFKNKYPESIVTLVSKDVNVRVKADCVGINAENYETDTIKYDEFFTGWEI
                     LDVEPEQYREIEEKGYINNIFGDFFANQFVRIPTPDNPDKYTILRYHKGRNQLYVIKH
                     YTGQYVYGIKARNFEQEMALDILLDDSIKLVSLSGKAGTGKTLLAIAAGLQKVVEEQK
                     YSRLVISRPISPLGKDLGYLPGSKSEKFNPWMQPIYDNMEILLSSHLDKNKPNSNTKK
                     RTSVNDLTDYGFLELEPLTYIRGRSLPDQFFIIDEAQNLSPHEMKTIITRAGENTKVV
                     LTGDPYQIDIPYLDSQSNGLSVSVEKFKGEILVGHITLDKGERSALADLAAKYL"
     gene            6221..>6429
                     /locus_tag="PKM80_03085"
     CDS             6221..>6429
                     /locus_tag="PKM80_03085"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV92921.1"
                     /translation="MIKKNICDRSWVEIDLSAFRKNLSILKSYLEPHQQFMMIVKADA
                     YGHGAIEISKIASEEGAVYFGVANP"
BASE COUNT         2023 a         1181 c         1132 g         2093 t
ORIGIN      
        1 cagaatgaaa gagtttgtag gaattatata aggaagacat tgaggcaggc caactattca
       61 ttccaatgta accccggaag ttataaaagg caacaccttg attaattgca ctattggttg
      121 ttgtattact ggggctggaa ccatagacct cagtataagt atatgcagga ttaatttctt
      181 ccgaaatatc acgaatgtat ctatttgtat aaatcgttga gatacctgag gagctggaat
      241 ctcctactaa aaccattcta tttaaccaat caataggatg ctggctgata ttcttttcca
      301 gagaaatgat ttttgccaaa attacatcca gatcagaagt agaattaacg gaaatcctgc
      361 caatcatcac atcacccaaa ttgtcatttc ccgccagcca ggtatattga taatccacat
      421 aagagctatt acttggcact gcaatatttc catctgtatc tccaattaaa gtaatataat
      481 cgggacgagt ggatgcattg ttataagcat tttgaatata actttttaca cttgtattgg
      541 atgttccggt aacactggtg ctaacagcgt ttacaataaa gcccttttgc tttttccagg
      601 taacatattc atttatcttt gcctgataga tcgcatctgc attattacca taaataacca
      661 gcatcacagg attcctgtaa ctaacattcc gtgtttgcct tacggaatca taatttaaga
      721 taagcccctt gtaaatacta tcaaaactgc aagaaacatc aatatccgaa ttcagttcgt
      781 tgccagctgt ccgattatca aaattcaggg ttatatccaa attttcacta ataaataggg
      841 tccggttatt ccggttatat tgaaagggat aaatgtttaa agttattact ctgaaatcac
      901 gcaatatctg tggctctgaa attaccacat tattggcagg ataaagaaca tcagaatatc
      961 taccctgttg actttctcgc atcaaagcat cattaaaatt agctgtaaat tcgttaattg
     1021 tggattttga actattggaa acaagaagat tcacccctcc cctattggga atagcaatca
     1081 ttgtagaaaa aacagggagt gtttcttctt catctataaa taaataggga gtatcttgaa
     1141 cttttatttg ttttctaatt tcacccttat catcaatttg attaatctgc cattcaggta
     1201 aagagaattg aatttttatc tctgaactac tggaatttat tatgctaaag gcatttttat
     1261 cactgccggt tttggcaaat attgctaatg gcaacagaaa aatggttact aatagcaatg
     1321 ttaaacattt agattgtttc atttataacc tctatatttt cgtcttctta gctataatgt
     1381 tgcaatatta gagccaattc ctgatttcct tatggctaca atgatttagt agattaactt
     1441 tttttttctt caagcagtga atgtgtatgc tgcaaatttc tcctatcttt gtaaccatat
     1501 actgcttatg aaattgcaat atcattcccg tatctgatac gggaacgata cgagaatgat
     1561 acgagaatta tatgagaatg atatgatcac cttaaaagaa aactggaagc gggttcagaa
     1621 tttataggaa aagaacatga gtatcgttag caggcaatta tttccttttc tataaacccg
     1681 aaacacgata aaatagcttg ccttttggct actgaattaa acgctatcca ctatgaaaat
     1741 atctatagtg ttttgaaatt aacctggttt cctgcttttg tataagggga agatagttgc
     1801 aattaagaaa agatatccca ctcattataa acaataggga taaattcctt tagcaacatc
     1861 ggtttcgttg ctaaaggatg aatgtgccta ctgcattata cccgagattg ctattagcaa
     1921 tagcagaagt tggataacct tcagctatac aattcgggtg ccggttttcc cttcaaaggc
     1981 atctacaatt ttatcaatgg aagtaatcaa cacttccgag ccaccgcgtt ccagaaaatc
     2041 aatagctgcc ttgattttag gacccatact gccggcagga aattgttttt ctgcataata
     2101 gcgttttgct tcttccacag ttaatttatc caagttccgt tgattttctt tgccgtaatt
     2161 aagtgaaacc ttttctacac cggttaaaat cacgaagaga tcggctttaa tttccaatgc
     2221 taaaagcgag gaagcaaaat ccttatcaat aactgcatcc acaccttcgt aagagccatc
     2281 atcttcttta taaattggaa ttccgcctcc gcctacggca ataactattt cacccttatc
     2341 tacaagttct ttaattgtag cggcaggaat tatttcaatg ggatagggcg aaggaacaac
     2401 acggcgaaaa ccttttccgg agtcctcttt aagcgtccaa cccaatttat tttttatttc
     2461 ttctgcctgc tcagcagtat agtaggtgct gccaacgaat tttgtaggat tcagcatact
     2521 gggatcattt ttatctacta caacctggga aatgatggtg ataacctgtt tattgatatt
     2581 gcttttatgc agtttattct gtaaactttg ttcaatcata tagcccatag tgccttctgt
     2641 agcagcattt aatacaccta aaggcaattg aggaattccc tccatttctc ctaattcctg
     2701 ttgacggagc agattcccta cttgaggtcc attaccatgt gttatactta aatgatagcc
     2761 ccttttgatt aattctacaa taccacctaa cgattcacgg gtatttgaaa attgttgtgc
     2821 tattgtacct ttttcaccag ctttaataat agcgtttccg ccaagtgcca gaacagcttt
     2881 tttttgcatt tctcaaatcc ttattgttct gtataaatta ttttgattga aaaaacaggt
     2941 taattctcat ttgtggaaca gagaattctc cctaatattc atagcgagaa gttaattgga
     3001 ggtggataag tacccacctc caatgtatta tcgattattt ttttgtagat ttcttgccga
     3061 agttctttaa gatgtcagca atagtaggaa caccgatttc ctgtaattca tagcttactt
     3121 ggcaggcagg tttatttatt ttaataattc tgcgaaggtc tatcggagta gcaattacta
     3181 cagaatcaca ttccgtactg ttaattgtct tttctaagtc tttaatttgc ttagaactat
     3241 atcccatagc ggggagcaaa gatccaactc cgggatattt ttcgaaagtc tgttttattt
     3301 caccaacagc ccatggacgg ggatctacaa ggtctgcaca accatatttt tctgctgcaa
     3361 caactcctgc tccgtattgc atttctccat gggttaaagt aggtccatct tccactacta
     3421 aaactttttt gcctaagatc atttcagggt gatctacaaa taaaggagaa gcagcctcaa
     3481 taatttgagc cgttggatta attgctctaa cattttctcg cacttcatta atatcgtaca
     3541 gattggcgct atcgatctta ttaataacga ttacatcagc caagtataca tttgtttcac
     3601 ctggataata agaaatctca tcaccaggac ggtgaggatc tacaactacg atcttaattt
     3661 cattatcgga agtgtagaaa ggaatatcat tatttccacc atcccaaaca ataacatccg
     3721 cttctttctc tgcttctttt aaaatagcag cataatctac acctgcataa attacactac
     3781 ccatcataat atggggctca tattcttcca tttcttcaat agtgcaattg tgctttttga
     3841 ggtcatcaag ttctgcaaaa cgctgaactt tttgttttac gaggtcaccg taaggcatag
     3901 gatgacgaat agagacaact ttcaatcctt tagctttgag agcttttacc actgttctgg
     3961 ttgtttgaga ctttccgcaa ccagttctta ccgcgcatac agtaaccaaa ggtttgctgg
     4021 ttttcagggt agtattatct ttgcccatca acataaaatc tgcaccagtg gagttaataa
     4081 ttgaggcttt atgcatcaca acttcgtaag gaagatcgct ataagctaaa actactaaat
     4141 cgacattgtg ttgcttaatc aaatttttaa tttcgctttc cggataaata ttaataccat
     4201 caggatagag ttttccagct aaagcagccg gatatttttt gtcgtcaatt ccaggaatct
     4261 gtgtagcagt aaaagctaca acttcataat ccttattatc gcggaagaaa acattgaaat
     4321 tgtgaaagtc ccttcctgct gcacccataa tgataacttt gcgtttcatt gatgaatctc
     4381 ctttatttaa tatttgttac aaatttgctt tcaaggttta aaatagggtt ttaccgtcaa
     4441 ggaaaatcta ttttttttga attactgaca tataatatct aaataccaat atgaaaatcg
     4501 ggcttgacaa aaaaaggctg tcttaaaaat agaaaaacaa atgtgctgaa gtggtgaaat
     4561 tggtatacac gctagtttca gggactagtg agcaatgagc tcatgggggt tcgagtcccc
     4621 ccttcagcac catttttttg tgcaccgaat tattttccct cataattatt cgagttagta
     4681 tgcgttaccc aggataaaat aatcattgac taacaatagc tcattatata agttgtttgt
     4741 tgtttcttac attgatacaa aactatttct ttctactttc gattaagaaa taagctctga
     4801 tttttcaaag ttcgattgga aagcagtttg cgaagaaatt agaaagcatt aatatatata
     4861 aaaaggaatg tatagaatgg aaaagaaaat ttttgtcctg gatacaaatg tactaattca
     4921 taatccgcaa gctctatttt cctttcagga taatagagtt gttttaccta ttgtggttat
     4981 tgaagagatt gatcaattta aaaaaggggt tgatgaaaaa agtaggaatg cccgtcaaat
     5041 tggtcgtttt cttgatgctt tacgcaaaaa agggagtttg caggaaggtg ttcctacaga
     5101 gaatggggga acaatacaaa taacgatcaa caaagaaata acggccacag cttccgagct
     5161 tttatttatg gaccgtaatg acaatttgat aatcagtaca gccttgtatt ttaaaaataa
     5221 gtatcccgaa tctattgtta ctttggtttc caaagatgta aatgttcggg taaaagcaga
     5281 ttgtgttggc atcaacgcag agaattatga aaccgacact attaaatatg atgaattttt
     5341 caccggatgg gaaatacttg atgtggaacc tgagcaatac cgtgaaatag aagagaaggg
     5401 atatataaac aacatctttg gggatttttt tgccaatcaa tttgttagaa tccctactcc
     5461 tgataatcct gataaatata caattttgcg ttaccataaa ggccgaaatc aactttatgt
     5521 aataaaacac tatactggcc aatatgtata tggaatcaaa gcccgcaatt ttgaacaaga
     5581 aatggcttta gatattcttt tggatgatag catcaagctg gtgagtttat ctggaaaagc
     5641 aggaactggg aaaacccttt tggctatagc agctggcttg caaaaggttg tagaagaaca
     5701 aaagtactca cgcttggtta tttctcgtcc catttctcct ttaggaaaag accttggcta
     5761 tttacctggt tccaaaagtg agaaatttaa tccctggatg caaccgatct acgataacat
     5821 ggagatactg ctatcttcgc atttagataa aaacaaaccg aattccaata caaaaaaaag
     5881 aaccagtgtt aatgacctta ctgattatgg ttttttagaa ctggaacctt taacctatat
     5941 cagaggacgg agcttacctg atcagttttt tataattgat gaagctcaaa atttgagccc
     6001 tcatgaaatg aaaacgatta ttaccagagc tggcgaaaac actaaagtag ttctaactgg
     6061 agatccttat cagatcgata taccctatct tgattcccaa agcaatggac tatctgtttc
     6121 cgttgaaaag tttaagggtg agatccttgt gggtcatatc actttagata aaggtgaacg
     6181 aagtgcttta gcagatttgg ctgccaaata tctataagct atgataaaaa agaatatttg
     6241 cgatcgcagc tgggttgaaa ttgatctctc tgctttcaga aagaatttaa gcattcttaa
     6301 aagttatctt gaaccgcatc agcaatttat gatgattgta aaggcagatg cttatgggca
     6361 tggagcaatt gaaataagta aaattgcttc tgaagaagga gcagtttatt ttggagttgc
     6421 caatccaga
//
LOCUS       DAORHB010000066         6393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_12112_length_6393_cov_4.388766, whole genome shotgun sequence.
ACCESSION   DAORHB010000066 DAORHB010000000
VERSION     DAORHB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12112_length_6393_cov_4.388766"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(462..1958)
                     /gene="lysS"
                     /locus_tag="PKM80_03090"
     CDS             complement(462..1958)
                     /gene="lysS"
                     /locus_tag="PKM80_03090"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNV92922.1"
                     /translation="MPDNSNQFIRLRKEKLEKIKSLGVDPYPIDSNRTHYISALLEDK
                     DNWIAQAEKITIVGRLVAMRRQGKIGFGNIEDISGKIQIYVAQNLIGEENYDLFKLCD
                     AGDFIQATGILFYTQTGEYSLKCNAIKLLSKNIRPLPAVKEKIVDGKKVRYDEFSDIE
                     LRYRKRYLDLLLNPEHRKVFETRAKIITAIRSFMDSRGYVEVETPILQPLYGGANARP
                     FVTHHNTLDMDLYLRIATELYLKRLIVGGFERVYELGKDFRNEGMDRTHNPEFTMIEF
                     YEAYSDLRGMMDIAEDLIKHLAMDVIGKDTFLYHNHQVCLNGNWKRVSMPDLVSEFVG
                     QDVMNMDFATLQAFCKHKGLEIPAGSEKGKLIALIFEEFVEKTLIQPTFVCDFPKEVS
                     PLAKAIPDNPFLADRFELYIAGGEFANAFSELNDPLEQRMRLEEQAKLRDLGIDDANV
                     VDEDFLEALEYGMPPMGGQGIGIDRLVMLLTENDSIKEVILFPQMKTT"
     gene            complement(1966..3477)
                     /locus_tag="PKM80_03095"
     CDS             complement(1966..3477)
                     /locus_tag="PKM80_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNV92923.1"
                     /translation="MPNKLREDSNKATMKEMKEEYLRMLEESFQNTAEIKKGDVVEAP
                     IVSIGDQYLILNLGGKFDAYAEIGEFSDSKGDLPYRVGDNLKGYVVDQNENGYVIAKS
                     LTRQYVDKQSILDAFEKKIPVQGKVNSPTKGGFNVDILGARAFCPISQISLHPVEDTA
                     QFIGKNLDFLVIECSENCRRLVVSHRQLEEMKEKELKEEALAKLKVGDVIKGKVLRMT
                     NFGAFIDLGGIEGLMHVSEISWQHVVKPQDELKKGQDIEVQILDIKGDKIALSRKALI
                     ENPFLQALNELHEGDTINCRILRLHSFGAFAELKPGVEGLIPISEMSRNRNISHPREI
                     VKEGDWVAVQILRIDEDTRKISLSLKALEEDPWDKIDEIIELDNPFEGIVESSTNFGV
                     FVTITDGVTGLLPRSRVRKNDSFKTGDPVNLKVTAIDKDNHRITLDYTDRTPEELFEE
                     QNRSANYNQRESRNENPRSGNQRRGSRNRNDEEWRKYANQKVNSAADNPFKDL"
     gene            complement(3514..6084)
                     /locus_tag="PKM80_03100"
     CDS             complement(3514..6084)
                     /locus_tag="PKM80_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNV92924.1"
                     /translation="MKEYSNLKQGLTTQEVEDKLKREGYNELPSSNARSIFKLMWDVI
                     SEPMFILLVSCAVIYFFLGDLQEALVLTASVVIVMSIELFQERKTEKALEALKNLSSP
                     RALVIRNSEQIRIPGREVVTDDLIILAEGDRIPADAYILENNSLSVDESLLTGESVPV
                     RKRIWNKEERPINPGGDDLPFVYSGTLVVSGRGIAQVFATGEKTELGKIGKSLEAITE
                     EETRLSKETGKLVKYFAAFALLICVVVVIVFYITRKHLVNALLTGITLAMSVLPEEFP
                     VVLTVFMALGAWRMSRKNVLVRRLQAIESLGSATVLCTDKTGTITYNKMTLQKIVSSD
                     ENIDLKSADKISNSIYTILYHSVLSSMREPFDPMEKAIHSALNEIEPSNYQELQNYQE
                     IKEYPLTDELRAMAKVWKLPDKTDYLVSLKGSPEAVIKLCALNSEEIEPILNQVSDLA
                     EEGLRVLGVAYCEVVPNVLPEKMTDLPFHYLGLIGLADPIRETVPEAVKMCYKAGIRI
                     IMITGDYIGTAKAIGKQIGLKNVDNIIVGSELDQMTDLELAEKCKQVSIFARVVPHQK
                     LRIVQALKANKEIVAMTGDGVNDAPALKAANIGISMGLRGTDVAREASAIVLTDDDFS
                     SIVSAVRMGRRIYDNLRKSFTYVFSLHIPIAGMAMIPAAFSNYPIIYFPVHIAFLELI
                     IDPACSIVFEMEKEEDDIMERPPRSIDEKLFGTRRLIIGALQGISVLILLVLIYFWGI
                     KSRMGDNHLRAHIFTTLVFSNLMLITTNRSWKKNLFQIIRTPNKGMTYLFLGVFTIVL
                     LMTQAPYITNIFHLESMSLLELLTCLVLGNISVAWFEIYKLIVNKKAKKQKPLQKEAL
                     "
BASE COUNT         1947 a         1309 c         1044 g         2093 t
ORIGIN      
        1 acaaaattag gattatgtct aatattcaga tgttagaata ggataaatct ccactgcatt
       61 gcagccaaaa aaatcaaaac ataactaact gttttaatta taattatgaa acactctaag
      121 aatataatcc tctcaatcct acaatcctga aatccttgtt tctaaaaatc aatccctaaa
      181 atctcccaaa tcttgttcag cccatatcta ttatcttaaa ttcgtcatcg tcgctattat
      241 tatgtcttct ttgaagggat ttttaataag cagtccgaag ttataagtca ggtatattgc
      301 atttacaaaa taggaaacat cattatagaa aggaggtaag cactgcttta atattcatat
      361 aatccaaagg acttgcttat ttgagtattt tttcacaata gccataaatt agagagatta
      421 tatccttgct tttatattga taaatggtgt tataaaattg attaggtggt tttcatttgg
      481 gggaaaagga taacttcttt aatagaatcg ttctccgtaa gtaacataac gagacggtct
      541 attccgatac cctgtccacc cataggaggc attccatatt caagagcttc cagaaaatct
      601 tcatccacaa cattggcatc gtcaattccc agatctcgta attttgcttg ttcttccaag
      661 cgcattcttt gttccaaggg atcattcaat tcgctgaagg cattggcaaa ttcacctccc
      721 gctatatata gttcaaagcg gtctgctagg aaaggattat cgggaattgc cttagctaaa
      781 ggagaaactt ctttaggaaa atcacaaacg aaggttggtt gaataagggt tttctctaca
      841 aattcttcaa aaatcaatgc aatcaattta cctttttctg agccggcagg aatttctagt
      901 cctttatgtt tacagaatgc ctgcaatgtt gcaaaatcca tattcattac atcctgtccg
      961 acaaattcgc taaccagatc aggcattgaa actcttttcc agtttccgtt taaacatact
     1021 tggtggttgt gatacaagaa tgtatctttg ccgataacat ccatagccaa gtgcttaata
     1081 agatcttcag caatatccat cattcctctc agatcagaat atgcttcata aaactcgatc
     1141 atagtgaatt caggattgtg agttctatcc atcccctcat tacgaaaatc cttacccagc
     1201 tcgtaaaccc gttcaaaacc tcccacaatt aatcgtttca gataaagctc tgtagcgatt
     1261 cttaaatata aatccatatc taaagtattg tgatgagtaa cgaaaggacg ggcatttgct
     1321 cctccgtaaa gtggttgtaa aataggggtt tctacttcta cataaccgcg actatccata
     1381 aaactacgaa tagcggtaat tatttttgct cgggtttcaa acacttttct atgctcggga
     1441 ttcagtaaaa gatcaagata acgcttacga taacgtaatt caatatcaga aaattcatcg
     1501 tagcggactt tcttaccgtc aactattttt tcttttacag ctggcaaggg tctgatgttt
     1561 ttagagagca gtttgatagc attgcatttc agagaatatt cacctgtttg agtgtaaaat
     1621 aaaattcctg tagcttgtat aaaatcacca gcatcacata gcttgaaaag gtcgtaattt
     1681 tcttctccta taagattttg tgctacataa atctgaatct tgcctgaaat atcctcaatg
     1741 tttccaaaac ctattttgcc ctgcctacgc atagctacaa gacgccctac aatagtaatt
     1801 ttttcagctt gtgcaatcca attatcttta tcttccaaaa gggcagatat ataatgagtc
     1861 ctattggaat caattggata aggatctacc cctagtgatt taatcttttc cagtttttct
     1921 ttgcgtaacc ggataaattg attagaatta tctggcatta ctattttata aatccttaaa
     1981 gggattatca gcagcagaat ttactttttg attggcatat ttacgccatt cttcgtcgtt
     2041 gcgattgcgt gaacctctgc gttgatttcc actgcgggga ttttcatttc gtgattctct
     2101 ttgattgtag tttgcagagc gattctgctc ttcaaatagt tcttcaggtg tcctatctgt
     2161 ataatcaaga gttattctat ggttatcctt atcaatagcg gttactttta aatttacagg
     2221 atcacctgtt ttaaagctgt cattcttacg aactcgagac cggggtaaaa gccctgtaac
     2281 tccatcagtg atggttacaa aaactccaaa atttgtagaa ctttctacaa taccttcaaa
     2341 gggattatcc agttcgataa tttcgtctat tttgtcccag gggtcttctt ccaatgcttt
     2401 caaagaaaga gatattttac gggtatcttc gtcaattctt aaaatttgaa cagctaccca
     2461 atcaccttct ttcacgattt cgcgaggatg cgaaatattg cgattacggc tcatttcgga
     2521 aataggaatt aaaccttcca caccaggttt tagttcggca aaagcaccaa aactatgtaa
     2581 acgcagaatg cgacagttaa ttgtatctcc ttcatgcagt tcgttcagtg cctgtaaaaa
     2641 aggattctct attaatgcct tacgagaaag ggcaatttta tcacccttaa tatccaaaat
     2701 ttgcacttca atatcctgac cctttttcag ctcgtcttgg ggcttaacaa catgctgcca
     2761 ggaaatttct gaaacatgca tcagaccctc aattccacct aaatctataa aagcgccaaa
     2821 attggtcatc cgcaaaactt tacctttaat cacatctcca accttcaact tggctaaggc
     2881 ttcttctttc agttcttttt ctttcatttc ttccagctgg cgatgtgaaa ctactaatcg
     2941 acggcaattt tctgaacatt caataacaag aaaatctaag ttcttaccta taaattgagc
     3001 tgtatcttca accgggtgta aggatatctg tgaaattgga cagaatgctc gtgctcccag
     3061 aatgtcaaca ttaaatcctc ccttggtagg agaattaact tttccctgca cagggatttt
     3121 tttctcgaaa gcatcaagaa tggattgctt atctacatac tgtcttgtta aacttttagc
     3181 aattacatat ccattttcat tttggtcaac aacataaccc tttaaattgt ctcccactct
     3241 atatggaaga tctccttttg aatcggaaaa ttctccaatt tcagcgtaag catcgaattt
     3301 gccgcctaaa ttaagaataa gatattgatc accgatgctg actatgggag cttcaactac
     3361 atcccctttt ttaatctcgg ctgtgttttg aaaggattct tcgagcattc gaagatattc
     3421 ttctttcatt tctttcattg tagctttatt gctatcttct cgtagcttgt tgggcataac
     3481 tatctcctaa aggcatttca gaaaaccacc ttttcagagg gcttcttttt gtaaaggttt
     3541 ttgtttcttt gctttcttat tcactatcag cttgtatatt tcaaaccaag cgacgctgat
     3601 gttccctaaa actaaacagg taagtaattc taatagagac attgattcta agtggaagat
     3661 attggttata taaggcgctt gagtcatcaa cagaacaatg gtgaaaactc ccaaaaataa
     3721 atatgtcata cctttattgg gagtgcggat aatctgaaac aagttctttt tccagctacg
     3781 atttgtagtg attagcatca aattggaaaa gaccaaagta gtaaaaatat gcgctctcaa
     3841 gtgattatct cccattctgc ttttaattcc ccagaaatat atcaacacta ataaaataag
     3901 aacgctaatg ccttgtaaag caccaataat taatcttcta gtgccaaaca acttctcatc
     3961 aatagagcga ggaggtcttt ccataatgtc atcttcttct ttttccattt caaaaacaat
     4021 agagcaggca gggtctatga ttaattctaa aaaggcaata tgaactggaa aatagataat
     4081 cggataatta gaaaatgcag caggtatcat tgccatccca gcaatgggta tatgtaaaga
     4141 aaacacataa gtgaaggact tccgcaggtt atcatatatc cttctaccca tccttacggc
     4201 acttacaatt gaagaaaaat cgtcatctgt taaaacaatt gccgatgctt cacgcgctac
     4261 atccgttcct cttaaaccca tagaaatacc aatattggct gctttcaaag caggggcatc
     4321 attaactcca tctcctgtca tagccacaat ttctttgttt gcctttaggg cttgcacaat
     4381 tcttaatttc tgatggggaa caacccgggc gaatatagat acctgtttac atttttctgc
     4441 caattccaga tcggtcattt ggtctaattc actaccgaca atgatattat ctacattttt
     4501 cagcccaatt tgtttaccaa tcgcttttgc cgttccaata taatctccgg taatcataat
     4561 aatcctgatg cctgctttgt aacacatctt aactgcctca ggaactgttt ctctaatcgg
     4621 atctgcaagt ccgatcaaac ccaaatagtg gaaaggtaaa tcagtcattt tttccggtaa
     4681 gacattggga actacttcac aataggcaac tcctaaaact cttagccctt cttctgccag
     4741 gtctgaaact tgatttagta tcggctcgat ttcttcagaa ttgagagcac agagtttgat
     4801 aactgcttca ggagatcctt ttagcgaaac aagataatct gtcttgtcag gtaatttcca
     4861 gaccttagcc atcgctcgta gttcatcagt taaaggatac tcctttattt cttgatagtt
     4921 ttggagctct tgataattag atggttcaat ttcatttaaa gcactgtgaa ttgccttttc
     4981 catagggtca aagggttctc tcatactgct caacacactg tggtaaagaa tagtgtagat
     5041 agagtttgag atcttatcag cactctttaa atctatattt tcatcgctac taacgatttt
     5101 ttgcaaggtc attttgttgt aagtaatagt tcctgtttta tctgtgcata aaactgtagc
     5161 tgaacctaaa ctttcaattg cctgcaatct tcttactaag acatttttgc gtgacatcct
     5221 ccatgcgcct aaagccataa aaacagttaa aacaaccgga aattcttccg gcaaaacaga
     5281 catagctaga gtgatacctg ttaataaagc attaactaag tgctttctag taatataaaa
     5341 aacgataact acgaccacac agataagcaa agcaaaagcg gcaaaatatt tcactaattt
     5401 acctgtctcc ttacttaagc gagtttcctc ttccgtgatt gcttccaggg atttaccaat
     5461 ttttcccagt tccgtttttt ctcctgtggc aaaaacttga gctatgcctc taccactaac
     5521 aaccaaagtg ccggaataaa cgaagggtaa atcatcacct cctggattaa ttggtctttc
     5581 ttctttattc caaatccgtt ttctaacagg tactgattca cctgttaaaa gcgattcatc
     5641 aacacttagt gaattatttt ccaatatgta ggcatcggct ggaatgcgat ctccttccgc
     5701 taatataatt aaatcatcag ttactacttc tctgcctgga attctgattt gttctgaatt
     5761 ccgaataact aaagcgcgcg gactggagag atttttcaat gcctccaaag ctttttctgt
     5821 tttgcgttcc tgaaaaagtt ctatagacat tacaataaca acggatgccg ttaggacaag
     5881 cgcttcttgt aaatccccta aaaagaagta gattacagca caggacacca atagaatgaa
     5941 cattggttca ctaattacat cccacattaa cttgaatata ctgcgcgcat tggaacttgg
     6001 taattcatta tacccttccc gcttcaattt atcttctact tcttgggtgg tcaaaccctg
     6061 tttcaagtta ctatattcct tcattttatc tcctcaaaca aaataaaatc ttggtatcag
     6121 caattatgtt tttagtcaag aaaaaccggt tcgtgtttag ggaagaaaag agaaaacggg
     6181 aaatattttt attagcactc tcacttttag gcattcccac aaaaaaacga tgtggataag
     6241 ttataacaca ctgttgtata gatatatatg ttgtgtaagt gtccaaggga ttcgttttgt
     6301 cattcgtggc aagggtgatc gcgctgttaa agaaggaata gaagaaatca atttcaataa
     6361 aatcgtaaac gaaatggagt tcaagtatga att
//
LOCUS       DAORHB010000067         6384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_12139_length_6384_cov_6.478907, whole genome shotgun sequence.
ACCESSION   DAORHB010000067 DAORHB010000000
VERSION     DAORHB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12139_length_6384_cov_6.478907"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PKM80_03105"
     CDS             <1..977
                     /locus_tag="PKM80_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV92925.1"
                     /translation="PARYYNVFMETGSCHLIEYFRRYTFETDDIPRDDSGLHHLLYIP
                     NQADYLSCLGASQRFNFYQMNSLPQTVVDGYKRFHLPLAADSEQILTAISSAQEKRSF
                     ISSSETRINILDPDNPENLLLNITLSNDSTTLFEWSSTSAIYPKFFAAIFEENNFENR
                     SLYTLKKWLVYNEPISSTINLHSSMEIDLIINTSELDSLKSYRVYYWLQNSYTNGGQV
                     FYAKCATLPYGWTVANSDDNLPRPNLKIYPNPLNNGQSLKISGFTSPSVFTIYNLKGQ
                     KIFTTGLINAEVNVPFEVFPASGIYFVQSETSERGKAIKQIKKISIIK"
     gene            986..1939
                     /locus_tag="PKM80_03110"
     CDS             986..1939
                     /locus_tag="PKM80_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV92926.1"
                     /translation="MTDRLTISELHQKVNQEISGLFLVAEKELREGKNDFYLRLKLQD
                     RSGSISANVWKDAAKLSEQFDAGDIINIQGTVVNYKGQTQLSVSKLRFADRSEYNIED
                     YLMRSKIAPEVLSERFFEFVDKVKQPYLNRLLHLIFDDKDFFTQFLTSPAAKSWHHNY
                     IHGLIEHSVSVAALCEFASTLYPVDYDLLISGALLHDMAKIKEYDSKSNIDFTDIGRL
                     IGHLTLSDQIVCETASKIAGFPEELLLNLRHLILSHHGEYEKASVRLPQTLEAVVLHH
                     CDNLDAQTVGVAQLIESAPKNAIWTEYDKLNNRYYKIYRPV"
     gene            1945..2730
                     /locus_tag="PKM80_03115"
     CDS             1945..2730
                     /locus_tag="PKM80_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV92927.1"
                     /translation="MFQTAIIASGSKGNCVLIQTENTALLLDAGISMHRILECLNFYN
                     IDLSKITGILVSHEHSDHIRSVGSISRKLKVPIYINRPTLSRCEQRLGNINDRIVIFN
                     TGSGFEVGDICVEAFISSHDAAESCNFVFSSVSEPNKKLGVATDLGYPTQLSVLKLSG
                     VSTLILESNHDERMLMEGPYDWNLKKRIHGQQGHLSNIQAVGLISNLIHPGLKNLILA
                     HLSEINNLPSLAEKTMKDYLESIRSDINLIVAEQNRPSPIIEI"
     gene            complement(2924..5293)
                     /gene="priA"
                     /locus_tag="PKM80_03120"
     CDS             complement(2924..5293)
                     /gene="priA"
                     /locus_tag="PKM80_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNV92928.1"
                     /translation="MYYYVIHLPLDIPKELVYCSSTEIVEGSRVLVNLSGRFWTGICG
                     RKTVPEEGKDIRYKIVTEVLDTSPVLSPELMKLGYWVAEYYHCAVGKALFAMLPSRLI
                     PEMEATITWLAQEIPESFISLKSALDKSENANFKNLRKLLPSYPLYKKIEEGEELGLL
                     KIERKLNHKDKPRTVNFIIRLEPILAEDILPLKQREAWERIKQEELSFPMANISSTIS
                     YSAIKALVKKGFIKIEPRRFDPESISFESSSSPKQIELNEGQLEAIKDILQGFGSFNV
                     NLLYGITGSGKTEVYIELIRYYLNKGKTVIFLIPEIALTPQMVDRFQSSFGQILAIQH
                     SQLTEKDRLTQWQKIKSGKKRIVIGARSAIFAPLPDLGLIIVDEEHESTYKQDSAPRY
                     QGRDLSVVRGQIQNAQVILGSATPALESWNNALSGKYKLQKLESRPLDYSLPEVQLVD
                     LRDEYDQSLISPILLEAIDKRLQKKEQVILFQNRRGYSSFMQCLKCGKLITCTNCEIS
                     MSYHRDREEMQCHYCGNTIPSPRKCPSCGSYSFAYGAPGTQKIEQNLKILFPEAKILR
                     MDSDSARKKDTYKSMYNRMKKREIDILLGTQMISKGLDFPLVTLVGIIMADISLNLPD
                     FRAAERTFQLLTQVAGRSGRSDKAGEVIIQTYNPDHYAIVSASQQNYINFATEELGYR
                     KKLYYPPYYRLARILFQSTDSELLKNEMAAMQETIYQLHKQYPVPQLLLLGPSVAPFS
                     KISSYYRYHIIFKAASVKILQSAIKMFNSLYTCPHHIKKQIDIDPNSLM"
     gene            complement(5390..5938)
                     /locus_tag="PKM80_03125"
     CDS             complement(5390..5938)
                     /locus_tag="PKM80_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV92929.1"
                     /translation="MKKLSSANYKDVNYCLRCGHKLELKTDSENKLRPHCSACNWVYY
                     KNPIPAVAIVLFNENNELLLVKRRNEPKAGFWALPSGYMEINLTPEENAIQELEEETG
                     LKGKIMHCVGWYYGDSPIYERILSIGFRMQVIGGKLQAGDDAEEAKFYPLYNLPVIAF
                     DAHRDFISKEIATHQVNLEACR"
BASE COUNT         2052 a         1150 c         1090 g         2092 t
ORIGIN      
        1 tccctgctcg atattataat gtctttatgg aaacaggcag ctgtcatcta attgaatatt
       61 ttaggcgtta tacttttgaa acagatgata ttcccagaga tgattcaggt ttacatcatt
      121 tactttacat tcccaatcag gcagattatc tttcttgttt aggcgcatca caaagattta
      181 atttctacca aatgaattct ttacctcaaa ctgttgtaga tggttataaa agatttcatc
      241 tacctcttgc tgctgattct gaacaaattc taactgctat ttcttccgca caagaaaaaa
      301 gaagttttat ttccagcagt gaaaccagaa ttaatatttt agatcctgac aatcctgaaa
      361 acttactttt aaatattacc ctaagtaatg acagcactac tctatttgag tggtcatcaa
      421 cttctgctat ctatcccaaa tttttcgctg caatttttga ggaaaataat tttgagaata
      481 gatcgcttta tactcttaaa aaatggctgg tttataacga accaataagc agcacaatca
      541 atctgcatag tagtatggaa attgatttga taataaatac ctctgaactg gattctttaa
      601 aaagctaccg cgtatattat tggctacaaa attcttacac taatggaggc caagtttttt
      661 atgctaaatg tgcaactttg ccttatgggt ggacggtggc taattctgat gataatttac
      721 ctcgtccaaa tttaaaaata tatcccaatc ctcttaataa tggacagtcc ttaaaaatat
      781 ccgggttcac ttccccatct gtttttacca tttataatct gaaaggacag aagattttca
      841 caacgggctt aataaatgca gaagtaaatg tcccctttga ggtttttccc gcttccggaa
      901 tatattttgt gcaaagcgaa acttctgaac ggggcaaagc tattaaacaa attaaaaaaa
      961 ttagtattat aaagtgatgg atgttatgac agatcgttta actatttccg aattgcatca
     1021 gaaggtaaat caggaaatat ccggtttatt tctggttgct gaaaaagaac ttcgcgaagg
     1081 caaaaatgat ttctatctgc gtttaaaatt gcaagataga agtggaagca tctctgctaa
     1141 tgtatggaaa gatgcagcca aactttcaga acaatttgat gctggagata taatcaatat
     1201 tcaaggaaca gtcgtaaatt ataaaggtca aactcaattg tctgtttcca aattgcgctt
     1261 tgccgataga tcagaataca atatagagga ctatttgatg cgcagtaaaa ttgctccgga
     1321 agttctcagt gaacgctttt tcgagtttgt cgataaagta aagcagccat atctgaatag
     1381 attattacat cttatctttg atgataagga cttttttaca cagtttttaa cttcgccagc
     1441 agcgaaaagt tggcaccata attatataca cggattaatt gaacattctg tttctgtggc
     1501 tgctttatgt gaatttgcct ccactctcta tcctgtggat tatgatttac taatcagcgg
     1561 agcattactt cacgatatgg caaaaataaa agaatacgac agtaaaagca atattgattt
     1621 tacagatata ggacgcctta tcgggcatct aactctttcc gatcaaatag tttgtgaaac
     1681 agcttctaaa attgcaggtt ttccagaaga gctgttgcta aatttacgac atttaattct
     1741 atctcaccac ggagaatatg aaaaagcctc tgtgcgtttg cctcaaacct tggaagcagt
     1801 tgttttacac cattgtgata atctcgatgc tcaaacagtt ggagttgctc aattgattga
     1861 atcagctccc aaaaatgcca tttggacaga atacgacaaa ctaaacaacc gttactataa
     1921 aatctatagg cctgtctaag aattatgttt caaacagcta ttattgccag tggcagtaaa
     1981 ggtaattgcg ttttaattca aaccgaaaat accgctctgc ttttagatgc gggaatcagt
     2041 atgcatagaa ttttggagtg tttaaatttc tataatatcg atctatccaa aattaccggg
     2101 atattagtaa gccatgaaca ctcagatcac attcgcagtg tgggttccat ttcccgtaaa
     2161 ctgaaagtac ctatttacat aaaccgacca actctatctc gctgtgaaca gagattgggt
     2221 aatataaacg atagaatagt tatttttaat actggttccg gttttgaagt tggtgatatt
     2281 tgcgtggaag catttatttc ttctcacgat gctgccgaga gctgtaattt tgtgttttcg
     2341 tctgtttcgg aaccaaataa aaaattgggt gtggcaactg atttgggata tccaactcag
     2401 ttgtcagttc taaaattaag cggagttagc actcttattt tagaaagtaa ccacgatgaa
     2461 agaatgctta tggaaggtcc ctacgattgg aatctgaaaa agcgcattca tggacagcaa
     2521 ggccatcttt ctaatattca agcagttggc ctgatttcta accttattca tccaggttta
     2581 aaaaacttga ttttagcaca cctgagcgaa ataaacaatc ttccctcttt agccgaaaaa
     2641 acaatgaagg actatttgga aagcatcaga agcgatatta atctgattgt ggcagaacaa
     2701 aatagacctt cacctattat tgaaatatag agtacattta gcaaaacctg attttcttct
     2761 tatcaactta ttctcttttt ctcctactcc tcccatcatt gaggactagt tgaaaacgag
     2821 ttgaggacag gttgatgagg agaggatgag gagatactaa tagggaagca attataaaca
     2881 aagcaattgt tttttttcga tattgcctaa gtgcccacta aatttacatt aaagaatttg
     2941 gatctatatc aatctgtttt tttatgtgat gagggcaggt gtaaagagaa ttgaacattt
     3001 ttatagcact ctgcagaatt tttacagaag cagctttaaa gattatgtga taacgataat
     3061 aagagcttat tttactgaag ggagcaacag aagggcccaa cagaagtaat tgtggcacag
     3121 gatattgttt atgaagctga tatatagtct cttgcatcgc tgccatttca tttttcagaa
     3181 gttcgctatc ggtactttga aaaagaattc tggctaaacg gtaatagggt ggatagtata
     3241 gttttttacg ataaccaagt tcttctgtag caaaattgat ataattttgc tggctggctg
     3301 aaacgatggc atagtgatcg ggattgtaag tttgaataat aacttccccc gctttgtcgc
     3361 tgcgtcctga tctacctgca acttgagtta gcaattgaaa tgttctttct gcagctctaa
     3421 aatcaggaag atttaaggat atatcagcca taataatgcc caccaaagta accagaggaa
     3481 aatctaaccc tttagaaatc atctgtgtcc ccaaaagaat atctatttct ctttttttca
     3541 ttcgattata catagattta taggtatctt ttttccgggc agaatcggaa tccattctta
     3601 aaattttagc ctccggaaac agaattttta ggttctgctc tattttttga gttcccgggg
     3661 caccataagc aaaagaataa gatccacaag aaggacattt tcgaggactg ggaatagtgt
     3721 ttccgcaata gtgacattgc atttcttcgc ggtcacgatg ataactcata ctgatttcac
     3781 aatttgtaca agtaattagt tttccacatt ttaggcattg cataaaagat gaatagcccc
     3841 ggcgattttg aaaaagaata acttgctctt ttttctgtaa ccgtttatct attgcctcta
     3901 aaagaatagg tgaaattaag ctttgatcat attcatctct taaatcaact aattgcactt
     3961 ccggcaaaga ataatccagt ggacggcttt ccaatttttg caatttatat tttccggata
     4021 aggcattatt ccaagattcc aaagctggag ttgcacttcc taaaataact tgcgcatttt
     4081 gaatttgacc tctaacaaca gataaatccc ttccctgata acgaggagca ctatcctgtt
     4141 tataagtact ctcatgttct tcatctacaa taattaagcc caaatctggc aaaggagcaa
     4201 aaatggcact acgcgctccg attacaattc tttttttccc acttttaatt ttttgccatt
     4261 gagttaggcg atccttttct gttaattgac tatgctgaat agccaatatt tgcccaaaac
     4321 tactttgaaa acgatccacc atttgaggcg ttagggcaat ttctggaata aggaaaataa
     4381 cagtcttacc tttatttaaa taatatctaa taagttcaat atagacctct gttttcccgc
     4441 taccggtgat tccatatagt aaatttacat taaaacttcc aaaaccctgt aaaatatctt
     4501 ttatagcttc aagctgacct tcatttagct ctatttgttt gggggaagaa gaactttcga
     4561 aagaaataga ttcaggatca aatcttcgtg gttcaatttt gataaagcct ttctttacta
     4621 atgccttaat ggcactgtaa gaaatagtgg aactgatatt tgccattgga aaagatagct
     4681 cttcctgttt aattctttcc caagcctcgc gctgttttaa aggtaaaata tcttcagcta
     4741 aaataggttc taaccgaata ataaaattaa ctgtgcgcgg tttatcttta tgattcagct
     4801 tacgctcaat tttcagtaaa cccagttctt ctccttcttc aattttttta tataaaggat
     4861 atgagggtag aagcttgcgt aaattcttaa aattggcatt ttcactttta tccaaagcac
     4921 ttttaagtga aataaaagat tctggaattt cttgtgcaag ccaagtaata gttgcttcca
     4981 tttcaggaat taaacgcgaa ggtaacatag caaaaagtgc tttcccaact gcacaatgat
     5041 aatattcagc tacccaataa ccaagtttca tcaattctgg agataaaaca gggctagtat
     5101 ctaaaacttc tgtaactatt ttatatctaa tatctttgcc ctcttctgga actgttttcc
     5161 ttccacatat acctgtccaa aaacgtcctg agagatttac taatactctg gatccttcca
     5221 caatttctgt ggatgaacaa taaaccaatt ctttcggtat atctaaaggt aaatgtatta
     5281 cataatagta cataaattta tccttttctg taaatgcaga ttgtccacct taaaatggat
     5341 ctgaaggtct ttaggtcttg agtctcgaaa aattaagaag atagatatct tatctacaag
     5401 cttcaaggtt tacttgatgc gtggctatct ctttggaaat aaaatcccga tgtgcatcaa
     5461 aggcaataac aggcaaatta tacaagggat aaaattttgc ttcttcagca tcatctccgg
     5521 cttgaagttt tcctccaatt acttgcatcc gaaaaccaat actaagaatt ctttcgtaga
     5581 taggactatc tccataatac caacctacac aatgcattat ctttcctttt agacctgttt
     5641 cttcttctaa ttcttgtata gcattttctt caggagtgag atttatttcc atatatccac
     5701 ttggcaaagc ccaaaaacct gctttaggtt catttcgacg cttaactaac aaaagctcat
     5761 tattttcgtt aaaaagtact attgccactg ccggaatggg atttttataa tatacccaat
     5821 tgcaagcaga acaatgggga cgaagtttat tttcgctatc tgtttttagc tcaagtttat
     5881 gcccgcaacg cagacaataa tttacatctt tgtaattagc agaactaagt tttttcatta
     5941 tttcactata gaaatagata ttatttttgt caagcagaac aagtgcatag aaaatgaatt
     6001 tatgtatgcc caagaactta gaatggatct tattattaca aatttacgag ctttttcaga
     6061 tgtagtaata atgaaatgaa tcgctttaca gagagtaaaa agataacgct cttccccaaa
     6121 aaaagtgttt tgaataactt taaggacaaa atactcatat ttcactaaac ggaggttgac
     6181 aaaatcaact aattcaaaaa tttgactaca taaacttgtg tggctctgaa taaaatgaca
     6241 gggtctgata gcaagaagga gtattatgat ggaaacatat aaagaccttg aggcttggaa
     6301 gaatggtatt gatttagcag taatggttta caaattaact agtgaattgc ccgaaagcca
     6361 ttcttttagt ttagccactg atat
//
LOCUS       DAORHB010000068         6371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_12176_length_6371_cov_4.543065, whole genome shotgun sequence.
ACCESSION   DAORHB010000068 DAORHB010000000
VERSION     DAORHB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12176_length_6371_cov_4.543065"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..470)
                     /locus_tag="PKM80_03130"
     CDS             complement(156..470)
                     /locus_tag="PKM80_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92930.1"
                     /translation="MTKQNKNRKGQTTLEEVVQSGSQLNLMPDDGTEQPIEEMLQDDP
                     LAHAYYYKSWWNQFRGPWLQSEKMTEDEIKTRADDAWECGDYKEMINLMAELICRLEK
                     KR"
     gene            1021..1374
                     /locus_tag="PKM80_03135"
     CDS             1021..1374
                     /locus_tag="PKM80_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV92931.1"
                     /translation="MKTLTCLCKSISEEEMDDIKKHIPKDNTIQNMAEFFKVFGDSTR
                     LKILYYLSRHELCVADLALLVNMQQSAISHQLKLLRLNRLVKYRKEGTTNYYSLDDEH
                     INSIFKVALEHLQEL"
     gene            1375..3438
                     /locus_tag="PKM80_03140"
     CDS             1375..3438
                     /locus_tag="PKM80_03140"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNV92932.1"
                     /translation="MIIEEYDVHNLDCPDCGSQIEKEINELKEVERANLDFVNKRLTI
                     EYIEQVNNPLKRLNAIASKIDPDVQFTLAGLELEQTEKKPWFLILAILILTISLFFSK
                     PISTILGFVAYLIAGHKVIYKAFREICSRRIFTEHFLMTLATLGALYLGEFLEAAAVM
                     ILYEIGNYLESGAIAKSRRNLKSLLSLKPETAHLKTENGINELKLNEIKKGQVILVYP
                     GERIPLDGVVIKGESTVDTSSITGEAIPVFVESKSKVFSGFLNGEGMLEIKVQNTEAE
                     SMVTRIIKLIENASSEKSETEKFMTRFARYYTPAVVLAAVFVFLIPTILGFNYTVWLR
                     RALIFLVVSCPCALVISIPLTYYIGIGKAAKKGIIFKGSVYLDLLSKVKTLIFDKTGT
                     LTTGELRIAEIEAAPNIDPEELKRSTWLCEYTSSHPFAKAIKNAFSYNFVPSAVEAFS
                     EYPGKGVLLVYNKDRYLCGSENFLQQFGFVSLLDLGDNSVVHTVKNDMYLGCISFVDE
                     LKPGMKEVLQSLKQKGIKHLSVLSGDRYPKVERVANELGLDSYYAELIPESKLEKMEE
                     FLKNSIDKVAYCGDGLNDAPVLARADVGIAMGKIGAQASIETADIVLLNDKPEQLESA
                     FSISRETNRMVWQNIVLALTIKLLVMTLGAAGLAKLWEAIIADVGVTLLVIFNSLRMM
                     KKPKC"
     gene            3564..5465
                     /gene="thrS"
                     /locus_tag="PKM80_03145"
     CDS             3564..5465
                     /gene="thrS"
                     /locus_tag="PKM80_03145"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNV92933.1"
                     /translation="MIKVTLPDGSIREYEKPVKALQIAQDFDPELAEKSLCAEVNDKL
                     VDITYTIDEDAKINFPTFETDEGKEVYWHSTAHLMAQAVKQLFPSTKLAIGPAIEQGF
                     YYDFDREESFTDEDLQKIEMKMKELIAQSLPYHRTELSKAEAIELFSEMNETYKLELL
                     EDIPEDEIISSYKQGDFIDLCRGPHLTDTGRIKAIKLLRTSGAYWRGDEKNKMLKRIY
                     GISFPTEKELADYLQFLNEAMQRDHRKLGKDLDLFSINDEVGPGLVLWHPKGAMVRHL
                     IEAYWKEQHLKKGYQLVYTPHIGRASLWETSGHLGFYKENMYSKMDIEGQDYYIKPMN
                     CPFHISIYNSSLHSYRELPIRLAELGTVYRYERSGVLHGLMRVRGFTQDDAHIICTPE
                     QMDSEVEKLIVFSLEMLKAFGFKEFLIYLSTQPEDCVGKKEDWDKATASLAASLEKLG
                     LEYKVDEGGGAFYGPKIDIKIKDALGRAWQCTTIQFDFNEPERFDMTYIGADNTAHRP
                     YMIHRAVLGSVERFFATLLEYHSGNLPLWLAPVQIILIPITDAQIEYTRKIEEQLLEQ
                     GFRCEFDARSEKMGYKIRAAEKSKIPFMCIIGKNEEANNQVTLRQHTVGDLGAMNLEE
                     AISILRQKL"
     gene            5462..>6371
                     /locus_tag="PKM80_03150"
     CDS             5462..>6371
                     /locus_tag="PKM80_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNV92934.1"
                     /translation="MNIYETVQEELAKEAFAIQIVAKQLSKESLEKAFELLCNCQGKV
                     VLTGMGKTGIIARKISATLASTGTTSIFLHSAEGIHGDLGMIESDDVVIAVSNSGNTQ
                     ELINLIPFLKFNRVPIIAITGEPNSQLAKNSDVVLNCNVPKELEPLGLVPTSSTTVAL
                     AVGDALAIALLKHKNFQLQDFARFHPGGTIGKKLLLRVINLMHSGDELPVIKDNATMS
                     EVIIEMTSKKLGCTAVTDKNGKLIGMITDGDLRRQLHNKGNSLLDYSAKDCMTDNPKT
                     LKPEVLAIEALNLMETYKITMIPVVDG"
BASE COUNT         2056 a         1076 c         1284 g         1955 t
ORIGIN      
        1 acgagtcata tcaggcggag cagaccgata acatcaaagc tctgcaggcc atgctcatgc
       61 tgtacaatgc cactatcttt tccgatcccc agggacgtat tgtgctcaag agagaaaccc
      121 ggcagaaccg ggttaatgaa tggcgtagat agtgattatc tctttttttc gagacggcag
      181 atcagctccg ccatcaagtt gatcatctcc ttgtagtctc cacattccca ggcatcgtcg
      241 gctctggtct tgatctcatc ctcggtcatc ttctccgact gcagccaggg acctcggaac
      301 tggttccacc agctcttgta gtaataggca tgggctaaag gatcgtcctg cagcatctct
      361 tcaattggct gctccgttcc atcgtcaggc atcagattaa gttgactgcc tgattgaacc
      421 acctcctcca gtgttgtctg tcctttgcga ttcttgtttt gcttggtcat tttgaactcc
      481 ttctcggtta cccgactgtt gatttatggg cgctactatg cacctctcgc aaaggtagtc
      541 aagtcctttc cgctcaatac gataaagaaa ctgcaagtat cttcattaac agaaatacag
      601 cccttgctca caaggaatac atcctacagg cactacatat aatgtcctcc gatatatggg
      661 aacaccactt agtgtctccc acattttttg ggaattccat ttgaagtttc tgtgataaaa
      721 gtttgaagaa atggtgataa aatttgaaga aatggtgatg atatttgaag aaatggtgat
      781 aaaaaatacc atccttggat actccagttg tgacctaatt tccacgattt gaagttttgg
      841 tgattaaatt taaagtttat agtgatggtt tatatctatt caacaatgct gcaagatact
      901 catcaacttg agctgttagg aaagattagt tgtaaatcga gcgatgaaat ataccaattt
      961 ttttcttgac aagcgtccct ccctagtaat atatgaacat atattcataa aggagctgaa
     1021 atgaaaacgc taacttgttt atgcaaaagc atttccgaag aagaaatgga tgatatcaaa
     1081 aaacatatcc ccaaagataa tactatccaa aatatggccg aattttttaa ggtttttggg
     1141 gatagtactc gcctgaagat tttatattac ctttcgcgtc atgaattatg tgttgccgat
     1201 ttagcgttac ttgtaaatat gcaacaatcc gctatttcgc atcagctgaa acttctccgt
     1261 ttaaaccgtt tggtgaaata tcgtaaagag ggcactacta attattattc tcttgatgat
     1321 gaacatatca acagtatttt caaagttgca ctggaacatt tacaggagct ttaaatgata
     1381 atcgaagaat atgatgttca taatttggat tgtcctgatt gcggttccca aatagaaaag
     1441 gaaataaacg aattgaaaga agtggaaaga gccaatcttg attttgtgaa taagcgtctt
     1501 accatagagt acatagagca agtgaataat cctctaaaac gattgaatgc catagccagt
     1561 aagattgatc ccgatgtaca atttacttta gcagggttgg agctggagca aacggaaaaa
     1621 aagccctggt ttttaatctt ggcgatatta attcttacaa tcagcttatt tttcagtaaa
     1681 cccatctcca ctatccttgg ttttgttgct tatctaattg ctggtcataa agttatttat
     1741 aaagcattca gagaaatctg ctctcgcaga atttttactg aacactttct gatgaccttg
     1801 gcaacattgg gagcattata tttaggggaa ttcctggaag cagcagctgt tatgattctg
     1861 tatgaaatag gtaattattt ggaaagtgga gcgatagcca aaagccgtag aaatttaaaa
     1921 tctcttcttt ctctaaaacc agaaacagca cacttaaaaa ccgaaaatgg cataaatgag
     1981 cttaagctta acgaaattaa aaaagggcaa gtgatacttg tttatccagg ggagcgaatt
     2041 cctttggatg gagttgttat aaaaggagaa tccactgttg atacttcttc cataacggga
     2101 gaagcaattc ctgtgtttgt agaaagcaag tccaaggttt tttctggatt tctaaatggt
     2161 gaaggaatgc tggagataaa agttcagaat acagaagcgg aaagtatggt tactcgcatt
     2221 ataaagctca ttgaaaacgc cagcagtgaa aaatctgaaa cagaaaagtt tatgacccgt
     2281 tttgcccgat attacactcc ggcagttgta ttggctgcag tatttgtttt tttaattccc
     2341 actatattag gttttaatta cactgtatgg ctacggcgcg ctttaatttt tctggtagtt
     2401 tcctgccctt gtgctttagt aatatccata cctctaactt attatattgg aataggtaaa
     2461 gcagcaaaaa aaggtatcat ttttaagggt agcgtgtatc ttgatctact tagtaaagta
     2521 aaaaccctga tatttgataa aaccggaact cttacaaccg gtgaattgcg tattgcagaa
     2581 attgaagcag ccccgaatat tgatccagaa gaacttaaac gcagcacatg gctttgtgaa
     2641 tatacttctt ctcatccttt tgcaaaagcg ataaaaaatg cttttagtta caattttgtt
     2701 ccttctgccg ttgaagcttt ttctgaatat ccaggcaagg gagtgcttct ggtttataat
     2761 aaagaccgtt atctttgtgg aagtgagaat tttttacagc aatttggatt tgttagttta
     2821 ctcgatttgg gtgataatag cgttgtacat accgttaaaa atgatatgta tctgggttgt
     2881 atcagtttcg tggacgagct gaaaccagga atgaaagaag ttctgcaatc gcttaaacaa
     2941 aaaggtatta aacatttaag tgttctttct ggagaccgct atcctaaagt agaaagagtg
     3001 gcaaatgaac ttggtttaga tagttactat gctgaattaa ttccggaaag taaactggaa
     3061 aagatggaag aatttttaaa aaacagcata gataaagttg cctattgcgg agatggttta
     3121 aatgatgctc ctgttttggc aagagctgat gtaggaattg caatgggaaa aattggagcg
     3181 caagcatcaa tcgaaacagc agatattgtt ttattgaacg ataaacccga acaactggag
     3241 agcgctttca gcatttctag ggaaactaat aggatggttt ggcagaatat agttctagct
     3301 ttaaccatca aattactggt tatgacttta ggagctgccg gactggccaa actctgggaa
     3361 gcgataattg ccgatgttgg agtaacttta ctggttatct tcaattcact tagaatgatg
     3421 aaaaagccga agtgctaata ctcttatata tcttttaagg tagtttctat tatcctggat
     3481 ttaaaatcat tctcggcttg acagaataat atttatagaa aaataggtta tttttttaaa
     3541 agaatattga aatggagtta taaatgataa aagtaacttt acctgatggt agtatccgag
     3601 aatatgaaaa acctgtaaaa gctttacaaa ttgctcaaga ttttgatccc gagcttgctg
     3661 aaaaatcact atgtgcagaa gtgaatgata agttggtaga tattacatac acaatagatg
     3721 aagatgctaa aataaacttc cccacttttg aaaccgacga aggtaaagaa gtatattggc
     3781 atagtactgc tcatttgatg gcacaggcag taaagcaatt gttcccttca acaaaacttg
     3841 cgattggacc agctattgaa cagggttttt attatgattt tgatagagag gaatctttta
     3901 cagatgaaga tttgcaaaag attgaaatga aaatgaaaga acttattgcc caatcccttc
     3961 cctatcatag gacagaatta tctaaagccg aagcaataga actttttagc gaaatgaatg
     4021 aaacatataa attggaactt ttagaagata tccccgaaga tgaaattatc agttcttata
     4081 aacaaggtga ttttattgat ctttgccgtg gaccacatct taccgatacg ggcagaatta
     4141 aagccataaa gttactaaga acttcaggtg cctactggcg tggtgatgaa aaaaacaaaa
     4201 tgctgaaacg aatttacgga atcagttttc ctacagaaaa agaacttgct gattatctgc
     4261 aatttttgaa tgaagctatg cagcgcgatc atagaaaatt gggaaaagac cttgatctat
     4321 tttcgattaa cgatgaagtt ggccccggac ttgtattatg gcatcctaaa ggagcaatgg
     4381 ttcgtcattt aattgaagca tattggaaag aacagcatct aaaaaaaggt tatcaattag
     4441 tgtatactcc gcatattgga agagcgagtt tatgggaaac cagtggacat cttggtttct
     4501 ataaagaaaa tatgtatagt aaaatggata ttgaaggtca agattattat atcaaaccga
     4561 tgaattgtcc gtttcatatc agtatctaca attcttcttt acacagctat cgggaattac
     4621 ctattcgcct tgcagaatta ggaacagttt acagatacga acgaagtggt gttttacacg
     4681 gtttaatgag ggttcgtggt ttcactcaag atgatgcaca tataatttgt actccagaac
     4741 agatggatag tgaagtggaa aaattgattg tcttttcttt ggagatgtta aaagcttttg
     4801 gctttaagga attcctaatt tatctatcta ctcaacctga ggattgtgtt ggtaaaaaag
     4861 aagactggga taaagctact gcttctttgg ctgcttcttt ggaaaagctt ggtttagaat
     4921 ataaagtaga tgaaggtggc ggcgcttttt atggtcccaa aattgatatt aagataaaag
     4981 atgctttggg tagagcttgg caatgcacta ctattcaatt tgattttaat gaaccagaaa
     5041 gatttgatat gacatatatc ggtgcagata atactgccca tagaccttat atgattcata
     5101 gagctgtttt aggttctgtg gaaagatttt ttgctactct actggaatat cacagtggga
     5161 atttacccct ttggcttgct cccgtacaaa taatactaat tcctataacc gatgctcaaa
     5221 tagaatatac ccgtaaaatt gaagaacaat tactggaaca aggtttccgt tgtgaatttg
     5281 acgctcgcag cgaaaaaatg ggatacaaaa ttcgcgccgc ggaaaagagt aaaatcccct
     5341 ttatgtgtat tatcggtaaa aatgaagaag ccaataatca agttacttta cgtcaacata
     5401 cagtcggtga tcttggtgct atgaatttag aagaggcaat ctctattttg agacagaaac
     5461 tatgaatatt tatgaaacag ttcaggaaga attagccaaa gaggcattcg ctatacaaat
     5521 agtggcaaaa cagcttagta aagaatctct ggaaaaagcc tttgaactgc tttgcaattg
     5581 ccaaggaaaa gttgttttaa ccggaatggg aaaaacaggg attattgcca gaaaaataag
     5641 tgctaccttg gcttctacag gaaccacttc tattttctta cattctgcgg aaggtattca
     5701 tggcgattta ggaatgatag aatctgatga tgtggttatt gctgtttcca atagcggaaa
     5761 cacacaagaa cttattaatc tcattccctt tctgaaattt aatcgggttc caattatagc
     5821 tattacaggt gaaccaaatt cacaattggc aaaaaattct gatgtagtcc tcaattgtaa
     5881 tgtccccaaa gaactcgaac ccttaggttt ggttccaact tcttctacta ccgtagcttt
     5941 ggcagtggga gatgctttgg ctattgcttt actaaaacat aagaacttcc agttacaaga
     6001 ttttgcccgg tttcatccag gtgggacaat tggtaaaaaa ttgcttttac gagtgatcaa
     6061 tttaatgcat agtggtgatg agcttcccgt gattaaagat aatgctacga tgtctgaagt
     6121 aataattgaa atgacttcta aaaaacttgg ttgtacagct gtaactgata aaaatggaaa
     6181 actgattgga atgataaccg atggtgattt aagacggcaa ctgcataata aaggtaattc
     6241 tcttttagac tattcagcta aagattgtat gacggataac cccaaaacgc taaaaccgga
     6301 agttttagct attgaagcct taaatttgat ggaaacatat aagataacaa tgatccctgt
     6361 tgtggatggt a
//
LOCUS       DAORHB010000069         6272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_12490_length_6272_cov_4.831269, whole genome shotgun sequence.
ACCESSION   DAORHB010000069 DAORHB010000000
VERSION     DAORHB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12490_length_6272_cov_4.831269"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PKM80_03155"
     CDS             <1..840
                     /locus_tag="PKM80_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV92935.1"
                     /translation="DLITRHFGEEPPLTGTRGSGTIFFSGCNLRCVFCQNYDISSNCW
                     GKDITKDELINLMFKLQTEGAHNINLVTPTHFTRQLREAIIYAKKEGLAIPIVWNSSA
                     YEKVETLQSLNGLVDIYLPDFKYAHKVYARKYSSASDYPAIAIAAIKEMFSQVGFLEV
                     DSNGIAKKGIIIRMLVLPYGLAGCKENLYTLANEIGVDLTLSIMGQYYPAGKANDYKE
                     LRRGISPKEYQDVIETAVALGFTNIYTQEISGSELWTPDFSPEPNNVYGGSEFNPIKE
                     QTI"
     gene            837..1958
                     /locus_tag="PKM80_03160"
     CDS             837..1958
                     /locus_tag="PKM80_03160"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV92936.1"
                     /translation="MKQKIITILMLISCLGMLFGNINIQIKGETKPHILKETTLASVP
                     YVELEEFCSVFKAVCKQDRTDNRLYLNIYDEQFIFLERSSYYTLKAVSYNMNYPLQRK
                     GERLYLPSTFVNEHLHTHFPAQVKLKGNTLQISKPIDNSVKTIVLDPGHGGKDPGAVG
                     RKLRANEKDINLAVAQKLKVLLEKELGITVLLTREDDRFVSLYDRTRFANEKKADLFI
                     SIHSNSSKSTSSKGIETYYLSTAQTSDARAVEALENAVVERFEGGSEAKKKYDDLDFI
                     LSDLLQTENLEHSNNMATSVHQNLVMGTQGIDRGVRQANFYVLRGAYMPSILVELGFI
                     SNAEEEQLLVNEEYQERLARTIFEGIKRFKFRYDRVKNT"
     gene            1978..3249
                     /locus_tag="PKM80_03165"
     CDS             1978..3249
                     /locus_tag="PKM80_03165"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HNV92937.1"
                     /translation="MSVVIQNILPKSLAAKNGIKSSEILLTVNGQEIRDFLDLELYTS
                     DYEFELEIMTPPGEKRIVKIQREEKSFLGIELEPYNIRSCENSCIFCFIDQMPPHLRN
                     TLYLKDDDYLYSYVFGNYITLTNLTNEDYERIITQRITPLYISLHTTDSTLRQKMMRP
                     LRQVDALSALKNLSQYGISFHIQIVCVPGYNDGEALKQTLKDLHQTKLNCLSIGIVPV
                     GLTKYRQNLCDLKTFNQHNAIEVLDLIEEARNLYSSDIIYPADEFFVLAGRNIPDEDY
                     YADYPQLENGIGMLRLTIQNYKLKKRSILKELRKKPVDYLMISSVCAKEVISKIAEDL
                     NKRLENQRIRVQVINNDFFGPDINVCGLITYSDLYSQLSPEKNETLILPSCIFNTEGE
                     TLDGKDRITFQETWNNPILLIDQFFEEWDYL"
     gene            3318..4244
                     /locus_tag="PKM80_03170"
     CDS             3318..4244
                     /locus_tag="PKM80_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV92938.1"
                     /translation="MLKRYILLTFIALLILGCGNQESRLEKANRKLGSKQNPIKMFFV
                     PSLEAGKVVSSGEAIAKYLENETKLHFKVAVPTSYAAVIEALGTYQADIAWLPTYAYI
                     LAKQKYDANVQLMTVRNGLHKYRGQFIARSDSDIKSLQDIQGKVIAYTDAASTSGYIY
                     PSAILKQNGITPKDYFFAGGHPQAILAVYSGRADVGCSYWSPSDSAGKPMDAREKLLD
                     TYPDVFEKVKIVGYTDWIPNDTVTFRKDLPPELQGLIVQTLYRYAQSPEGKKVLKTLY
                     DIDGLEYATDEDYEIVRTTLKSMNMDPADLLK"
     gene            4241..4993
                     /gene="phnC"
                     /locus_tag="PKM80_03175"
     CDS             4241..4993
                     /gene="phnC"
                     /locus_tag="PKM80_03175"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HNV92939.1"
                     /translation="MNSKNKLLEVINLNKSYDGHILALNNLSLQVQEGDFIILLGLSG
                     SGKSTLLRCINRLIEPSSGHILFREQNILPLKGIELRHYRRQIGMVFQQFNLVKNLTV
                     LTNVLTGRLGYHSSLSTFSKEDYELAMQNLEKVGLQNYANKKVKFLSGGQQQRVGIAR
                     ALMQNPSLILADEPVASLDPATADSIMQYLGEINKDGITIICSLHFLTLARRYGNRVL
                     ALKEGKKVFEGIPAEIDNFRFKEIYGQDAEEI"
     gene            5105..5311
                     /locus_tag="PKM80_03180"
     CDS             5105..5311
                     /locus_tag="PKM80_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92940.1"
                     /translation="MNNLLYFLLSINSLLYGIEGKRNVYIDLLFLFKAQKNRFIIKKQ
                     RALLQKFTNTYTADKRIGAEQSGL"
     gene            5850..>6272
                     /gene="rimP"
                     /locus_tag="PKM80_03185"
     CDS             5850..>6272
                     /gene="rimP"
                     /locus_tag="PKM80_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNV92941.1"
                     /translation="MEFYRTQIEEIAKKICQEMHLALYDIDEKMTGKGRVITVYLTKI
                     GGVTLDECASFSRALGKELETFDLIPERYFLEVSSPGLERPLKLKSHYVSAISEKVAV
                     QYSEGEEKKSVMGTLIEVNQDTIVLDDRGTIIEIPFKSI"
BASE COUNT         2150 a         1076 c         1179 g         1867 t
ORIGIN      
        1 gatttaatta ctcggcattt tggagaagaa cctcctttaa caggaaccag aggtagtgga
       61 actattttct tttcgggatg taatttacgc tgtgtttttt gccaaaatta tgatatctca
      121 tctaattgct ggggaaaaga tataactaaa gatgaactta ttaatctgat gttcaaacta
      181 caaacagaag gtgcacataa tataaatttg gtaactccta ctcactttac tcgtcaacta
      241 cgagaagcca ttatttatgc taaaaaagag ggattagcta ttcccatagt ctggaattcc
      301 tccgcctatg aaaaagtgga aactttacag tctctgaacg ggctggtaga tatatattta
      361 cccgatttca aatatgcaca taaggtatat gcacgaaaat attcttctgc atcggattat
      421 cctgctattg ctattgccgc tattaaagaa atgttctcac aagtaggatt tttagaagtt
      481 gatagtaatg gaatcgccaa aaaaggtatt attattcgga tgctggtttt gccctatggc
      541 ttagcaggtt gcaaagaaaa cttatacaca ttagcgaatg aaattggggt agatttaacc
      601 ctctccatta tgggccaata ctatcctgca ggtaaggcca acgattataa agaattacgc
      661 agaggaatat ctccaaaaga atatcaagat gtgatagaaa cagctgttgc tcttggtttt
      721 acaaatatat atactcagga aatttccggt tctgaactct ggacaccaga tttttcacct
      781 gaaccaaata atgtatatgg tggttcagaa tttaacccta taaaggagca aactatatga
      841 aacaaaaaat aattacaatc ttaatgctga ttagctgcct agggatgctt ttcggtaata
      901 taaatatcca gattaaaggt gaaacaaaac cccacatttt gaaggaaaca actcttgctt
      961 cagtccctta tgtagaattg gaggaatttt gcagtgtttt caaagccgtt tgtaaacagg
     1021 atagaacgga caatcggctt tatttaaaca tctatgatga gcagtttatc tttctggaaa
     1081 ggtcatccta ttatactctg aaagcagtat cctataatat gaattatccc ttacagcgaa
     1141 aaggtgaacg cttatattta ccttccactt tcgtaaatga acatctgcat actcattttc
     1201 ctgcacaagt aaagctaaaa ggaaacacgc tgcaaatatc aaaaccaatt gataacagcg
     1261 taaaaactat tgttctagac ccaggtcatg gagggaaaga tcctggtgca gtaggtagaa
     1321 aattaagagc aaatgaaaaa gatatcaatc ttgccgttgc ccaaaaacta aaagtactgc
     1381 tggaaaagga attgggaatt actgttttac taacaagaga agatgaccgt tttgtttctc
     1441 tttatgatag aactcgtttt gctaatgaaa aaaaggcaga ccttttcata agtatacatt
     1501 caaactccag taagtcaaca agttctaaag gaattgaaac ttattatctt tcgaccgctc
     1561 aaacttctga tgccagagct gtagaagcat tagaaaatgc agttgtagaa cgctttgaag
     1621 gcggcagcga agcaaagaag aaatatgacg atctggattt tatattaagc gatcttctac
     1681 aaaccgaaaa tctggaacat agcaataata tggctaccag cgtgcatcaa aatttggtta
     1741 tgggtaccca aggtatagat agaggagtta gacaggcaaa tttctatgtg ttacgaggtg
     1801 cgtatatgcc atccattttg gttgaacttg gttttatcag caacgctgaa gaagaacaat
     1861 tattggtgaa tgaagaatat caagaacgct tggctcgcac aatctttgaa ggaatcaagc
     1921 gttttaaatt ccgttacgat agagtaaaaa acacttaaaa gataatcctg aaaaactatg
     1981 tccgttgtaa ttcaaaacat tcttcctaaa agtttggcag ctaaaaatgg aatcaaaagc
     2041 agcgaaatac tgcttacggt taatggacag gaaatacgcg attttttgga tttggaatta
     2101 tacacatcgg actatgaatt tgaactggag ataatgactc ccccaggtga aaagcggata
     2161 gtgaaaattc agcgagagga aaagagcttt ctgggaattg aactggagcc atataatata
     2221 cgcagttgtg aaaattcctg catattttgc tttatcgacc agatgcctcc tcaccttaga
     2281 aataccctgt atctgaaaga tgatgactat ctttattcct atgtgtttgg taactacata
     2341 acacttacca atcttactaa tgaagattat gagcggataa taacgcaaag aattactccc
     2401 ttatatattt ccttgcatac aaccgatagt actttgaggc aaaaaatgat gcgtcctctg
     2461 cgacaagtgg atgctctatc cgctttaaaa aatttaagcc aatatggaat cagctttcat
     2521 attcaaattg tgtgcgttcc gggctataac gatggagaag cattaaaaca aaccttaaaa
     2581 gacctgcatc aaacaaaact aaactgcctc tcgattggaa tcgttcctgt tggtttaacg
     2641 aaatatagac aaaacctttg tgatttgaaa accttcaatc agcataacgc tatagaagtt
     2701 ttagatttga tcgaagaagc cagaaatctt tatagcagtg atattattta tcctgccgat
     2761 gagttttttg ttttagccgg ccgaaatata cctgatgaag attactatgc agattaccct
     2821 cagttggaaa acggaatcgg tatgctgcgg ttaaccatac aaaattataa actgaaaaaa
     2881 cgttcaatcc tgaaggaact acgaaaaaaa ccagtagact acttaatgat tagttctgta
     2941 tgcgctaaag aagttatatc caaaattgca gaagacctca ataaacgtct ggagaatcaa
     3001 cggattcgcg tccaagtaat taacaatgac ttttttggcc ccgatattaa tgtttgcgga
     3061 ttgattacct attccgattt gtattctcag ctttctcctg aaaagaatga aacccttatt
     3121 ctaccctcct gcatttttaa taccgaaggt gaaaccctgg atggaaagga ccgcattact
     3181 tttcaagaaa cctggaataa tcctatttta ttaatagacc agttctttga agaatgggac
     3241 tatttataat ttttacttga cctcatcctg cagataaaaa gatagttttt tacatgagat
     3301 taaaggagat ggtgtttatg cttaaaagat atatattgct tacctttata gccctactaa
     3361 tattaggttg tggcaatcaa gaaagccgtt tagaaaaagc taaccgaaaa ctgggttcca
     3421 aacaaaaccc cattaaaatg ttttttgtcc cttctttgga agcaggaaaa gtggtttcca
     3481 gtggagaagc tatcgccaaa tatttagaaa acgaaactaa attacatttt aaagttgctg
     3541 ttccaacaag ctatgctgca gttattgaag ccctgggaac ctatcaggca gacattgcct
     3601 ggcttcctac ttatgcctat attttggcaa aacaaaagta tgatgccaat gtgcaattga
     3661 tgacagtgcg aaacggttta cacaaatacc ggggtcaatt tatagcccgc agcgatagtg
     3721 atataaaatc cttgcaggat attcaaggca aggtaattgc ctatacagat gcagcttcaa
     3781 cttccggtta catctatccc tctgctatac ttaaacaaaa tgggatcact cctaaagatt
     3841 attttttcgc gggaggtcat cctcaagcta ttttagctgt ttatagtgga agagctgatg
     3901 taggatgtag ttattggtca ccgtcagatt cagctggaaa acctatggat gctagagaaa
     3961 aactcttaga tacctatcct gatgtgtttg aaaaagttaa aattgtcggt tatacagatt
     4021 ggatcccaaa tgatactgta acttttcgta aagaccttcc tcccgaacta caaggtttga
     4081 ttgttcagac cttatatcgc tatgctcaaa gccctgaagg gaaaaaagtt ttgaaaacac
     4141 tttatgatat agacggcttg gaatatgcta cagatgagga ttatgaaata gttcgcacta
     4201 ccttaaaatc tatgaatatg gaccctgctg atttattgaa atgaattcta aaaacaagct
     4261 gctggaagtt ataaacctaa acaaaagcta cgatggacat attcttgcct taaacaatct
     4321 atccttacaa gtacaggagg gtgattttat tattctgctg ggactttcag gaagcggaaa
     4381 atcaactttg ctgcgttgta taaaccgctt gatagaacca agcagtggac atatcctctt
     4441 ccgggaacaa aatatcctac cccttaaagg aattgaactt aggcattaca gaagacaaat
     4501 tggaatggtg tttcagcagt ttaacttggt taaaaacctt acagtgctta ctaatgtttt
     4561 aactggacgc cttggctatc attcttctct ctcaactttt agtaaagaag attacgagct
     4621 tgctatgcaa aacttggaaa aagtgggtct gcaaaattat gccaataaaa aagtgaaatt
     4681 cctctccgga ggacaacaac aaagagttgg tatagcccgt gccctgatgc aaaatccctc
     4741 tttaattctt gccgatgaac cagttgccag tttagatcct gccactgcag attccattat
     4801 gcaatattta ggagaaataa acaaagatgg aatcaccatt atctgttctt tacatttttt
     4861 aaccctggca agacgatatg gaaacagagt tcttgccttg aaagaaggta aaaaggtctt
     4921 cgaaggcatt cctgccgaaa tagataattt ccgcttcaaa gaaatctacg gtcaggatgc
     4981 cgaagagata taaataataa cctttgttag gaccttaagt aattatttat ttaactataa
     5041 ctggtttttt cacctatcca aattgagact aaaaccaaca aaaaccgtta cattcttcga
     5101 ataattgaat aacttgttgt attttctgct ttcaattaat tctttactct acggtattga
     5161 gggtaaaaga aatgtataca tagacctgct ctttcttttc aaagctcaaa aaaacaggtt
     5221 tataataaaa aagcagagag ctctgttaca gaaatttact aatacatata cggctgataa
     5281 aagaattgga gctgaacaat caggattata ggactttttt tagttggaag attaagtaag
     5341 gcgctatttg taccttcaga attataccat tctatgaaca ttttgagtaa atgtaaaata
     5401 atgatgagag tggtaattcc agatgtacaa aatatatccc tcatacctaa aataataaat
     5461 tactaattta ctatttatca actttcacta caaagcttta acctaatttc gaagatttta
     5521 cttgacagtt aatacccata ttttgataaa ggatttatgt atagaatccg ataaataaat
     5581 cggtgcaaaa gaagcggaaa ccgttaacta cggaaataga tagctatatt ggatagctat
     5641 tttttttgag gtgaaaagat atagatgttt aataataatt agttaaaggt tgatatgttt
     5701 ataggaatat gcaaagtaaa gttggcaacg ctaaacccta ataaggctaa gcaaattatt
     5761 aacataatct ataagaaaag aataactcag ttacctgaag caatacagga tgttgttcat
     5821 caataaaaaa ggaatgagga aaaaaaagaa tggagtttta cagaactcag attgaagaaa
     5881 tagccaagaa gatttgccag gaaatgcatc ttgctttgta tgacatagat gaaaagatga
     5941 ctggcaaggg acgggtaata actgtctatc ttactaagat tgggggcgtt acattagatg
     6001 aatgtgccag ttttagtcgt gccttaggta aggaactgga gacctttgac ctcattcctg
     6061 aacgttattt tttagaggtt tcttctcctg gattagagcg tccattgaag ttgaagagtc
     6121 attatgtgag tgccatttct gaaaaagtgg cagttcaata tagtgaagga gaagaaaaga
     6181 aatctgttat ggggacactg atagaagtaa atcaggacac gattgttctt gatgacagag
     6241 gcacgataat tgaaattccg tttaagtcta ta
//
LOCUS       DAORHB010000070         6239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_12593_length_6239_cov_5.335220, whole genome shotgun sequence.
ACCESSION   DAORHB010000070 DAORHB010000000
VERSION     DAORHB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12593_length_6239_cov_5.335220"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..3633)
                     /gene="mfd"
                     /locus_tag="PKM80_03190"
     CDS             complement(211..3633)
                     /gene="mfd"
                     /locus_tag="PKM80_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNV92942.1"
                     /translation="MLYSKIEEQLLQSEFMQGVQKLPLKDRSIQIYHLNQSARALIAA
                     HLWKQTGKNVIIVSQDDIIAEEIWDDLCVLIGKENAYYLPDYEILPYEERSPHYSIRA
                     TRMETLLSTFQNSTSGIYSLSIRALGRFLPSRDLLGRHILHLQQGMEYDPDVLLHKLY
                     DMGYDIQYQVSKVFQAARRGGIIDVFSPPLIHPVRLEFWGDEIIGMRLFSIATQRSVE
                     EYLTEFTILPARELAISDINSGSPIIAKIREKGFFEGIENYYALLCKELQTFAGYFEK
                     DNRILVWNNYYYIREEYETLFEQAISTWQKEAKIQGRARIPLPEKMFADLDTLTKIKS
                     GSDNLYLSQSEFELSFPTQNLRAPIESQPNFESDLGFLTKTLMEKQKKGWNNILLFDN
                     QSQAKRMQQTIYPSSENRNPSEQLPEIPFTEYIGVLHEGFSIEDCRLILYTDHEIFNR
                     YKGKRYTPRYAPGETIVNYEDLKPGDYVVHIDHGIGIFEGLKIISLDGSDVECLVLRY
                     ANDDRVYVPTYQLSLVTKYVAEESAKPVLNKLGSSKWNNTKRKAAQQIELIAADIVKL
                     YAERSSRIGIAHQADSEWQKEMEESFIYEDTPDQIRASKEIKEDMELPAPMERLLCGD
                     VGFGKTEVAIRAAFKAVCSGYQVAVLAPTTLLVEQHYRVFRERLIQYPVKIAMFSRFR
                     VPSEMKKDLLSIKSGSIDIAIGTHRLLSRDVQFKKLGLLIIDEEHRFGVRHKEKLRAL
                     QSNVDTLYMSATPIPRTLNMALSKLKEISLMQTSPKERLPVRTIITPRDMEVIKDAIR
                     REIDRGGQVFFIHNRVQTIETIASELRAAMPKVRFIVGHAQMPEQQLEKVMDAFLAKE
                     YQVLISTTIIENGIDIPNANTILIDNADNFGLAQLYQMRGRVGRSNRRAYAYLLISKG
                     TTTVARKRLEALTQYDYLGAGFQVALRDLELRGAGTVLGTKQSGIIQAIGFNYYNRIL
                     GNAIQAVEKGETSNLFNDETPETRRKVKTEIDLYFPPGYIDDDEERLKIYRRLSELET
                     FEEIDEFEIELLDRFGQMPDQARWLLNYFKLSQLSNKLALRNCQVKHDNLILEFDAKS
                     LPSKEQLLHFISKIDQPFRFEAGKNLKVIIDLNPDANYLQQFETALSILQKW"
     gene            complement(3789..5936)
                     /locus_tag="PKM80_03195"
     CDS             complement(3789..5936)
                     /locus_tag="PKM80_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV92943.1"
                     /translation="MNNRQLEVVTDTEYPVLVLAGAGSGKTRSLIYRTAYLIKEKHIH
                     PWNILVVTFTNKAARELQDRLEKLLNIPMRTLWVGTFHSLCCRILRFESAHLPFNSNF
                     SIYDEDTQVSLLKRIYKEYGFDKQKFPINRMLNRISRYKNKLMLPEDIQNPDDEKDSE
                     FATFINIYRLYQQQLLLNQALDFDDILLWTAKLLISNNAVREKYRSQFQYVMIDEYQD
                     TNKAQFEIVYQIAAGHQRVCVVGDDDQAIYSFRGASLRNILEFERDYTNVHSIRLEQN
                     YRSTGNILALANAVIANNRRRHNKELWSELPTGEKPILAVYENEIEEANKITEEVVNL
                     QNKGIPLREIAVLYRTNAQSRVFESALIQAEIPYSIVGGVQFYQRKEIKDLLAYLYVL
                     SNVNDNESLLRIINEPTRGIGQTSINRLISFANKTQMSLYKALQYADAVEELKNPARN
                     RISEFYLLMERWHNSITQKPALELVKTIVEELELIERYKNSNDPREIARAENIVEFVT
                     SVSEFSEHFYRENDRLPLIEDYLPYVALQTDMDRMKEEVDSLRLMTLHNAKGLEFEMV
                     FIVGLEDELLPHRMSMNTRENIEEERRLFYVGITRAKNYLHLSYAKTRRLYDTFYFPV
                     RSQFLAEIDSELYYLTQGDGGIAAPPKRMKKAGKVITENQKHYKIGQKVYHSEYGIGV
                     ILSVDGDGPDARLTISFGNRLAKIIGSYVEVTK"
     gene            complement(5967..>6239)
                     /locus_tag="PKM80_03200"
     CDS             complement(5967..>6239)
                     /locus_tag="PKM80_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92944.1"
                     /translation="TADQDLRVVTIRWVANSPYNTDGYYVYRGIGYNSSFALVGTVTI
                     NEFTHSSANDPSIQPGYYWYRVSAYKVYPEGKLEGRRSEPFSVKVP"
BASE COUNT         1841 a         1251 c         1129 g         2018 t
ORIGIN      
        1 aactcaaagg acaacacaaa tagcgatggt ggcgtaagcc cctcgaaaag aaagatatat
       61 aatgaaaaaa gttccgcttg gaacgataca tactagcgac gggtttttaa cccggcgata
      121 aaaagaatgc taaaaaaaag tcccagcggg acgacagatt tgagcgacag aattttaacc
      181 tggcgcatag ctaatagcca actaattctc ttaccacttt tgcaaaatac tcagggctgt
      241 ttcaaattgt tgtagataat tggcatcagg gtttaaatct attattactt tcaagttttt
      301 tccagcttca aaacggaagg gctgatcgat tttagagata aagtgtaata gttgctcttt
      361 agatggcaaa cttttggcat cgaattccag aatcaagtta tcgtgtttaa cctggcaatt
      421 tctaagagcg agtttattgg ataattggct caatttaaaa taatttagca gccagcgtgc
      481 ttgatcgggc atttgaccaa agcggtctaa cagttctatt tcaaattcat ctatttcttc
      541 gaaggtttcc aactcactca agcgtcgata gatttttagc cgttcttcgt catcatctat
      601 ataaccgggt gggaagtaga ggtctatttc ggtttttact tttcttcttg tttccggagt
      661 ttcatcgtta aataaattgc ttgtttctcc tttttccact gcttgaatgg cgttacccaa
      721 aatgcggtta taatagttaa agccaattgc ttgaatgata ccgctttgtt tggttcccag
      781 aactgtacct gctcctctta attccagatc gcgtaaagca acttgaaaac cagcacccaa
      841 ataatcgtac tgcgttaaag cttccagtcg ttttctggca actgtagtag ttcctttgga
      901 aataaggaga taagcataag ctcgtcgatt gctgcgtcct actcttccac gcatttgata
      961 aagttgtgct aagccaaaat tatcggcatt atcaattaga atagtattag cattggggat
     1021 atctattcca ttttcgataa tcgttgtgga aataagcact tgatattctt tagccaaaaa
     1081 ggcgtccatc actttttcca actgctgttc tggcatttga gcatggccaa caataaagcg
     1141 aactttgggc atcgcggctc gtaattctga agcgatagtt tcaatggttt ggacgcggtt
     1201 atgaatgaaa aatacctgtc caccgcggtc aatttctctt ctgatggcat ctttgatcac
     1261 ttccatatct cgaggagtaa taattgtgcg gactggtaat ctttccttag gcgaggtttg
     1321 catcaacgat atttctttca gtttagatag tgccatattt aaagtgcgcg gaatgggggt
     1381 tgcgctcata tatagggtat ctacattact ttgcagagct ctcaattttt ctttatgacg
     1441 gactccaaaa cggtgttctt catcaataat gagtaagcct aactttttaa attgaacatc
     1501 tcgagaaaga agacgatgtg ttccaatggc gatatctatg gagccacttt taatgctaag
     1561 aaggtctttt ttcatttctg agggtacacg aaaacggcta aacatagcaa tttttaccgg
     1621 atattggatt aagcgttctc taaaaacccg ataatgctgt tcaaccaata aagtagtagg
     1681 tgctaaaacg gcaacttgat agccactgca aacggcttta aaagctgctc ggatagcaac
     1741 ttccgttttc ccgaagccaa catctccaca tagcaatctt tccatcggtg cgggaagttc
     1801 catatcttct ttaatttctt tgctagccct aatttgatcg ggagtatcct catagataaa
     1861 tgattcttcc atttcttttt gccattcgga atctgcttga tgagcaatcc ctatgcgcga
     1921 actgcgttct gcataaagct taactatatc tgcggcaata agttcaattt gttgagcagc
     1981 tttacgcttt gtgttattcc atttagagct acccagtttg tttaaaacag gtttggcact
     2041 ttcctcagct acatatttag taaccaaaga gagctgatag gtaggcacat aaactctatc
     2101 atcattagca tagcgtaaaa ccaaacattc aacatccgag ccatccaaac taattatttt
     2161 aagtccttca aaaatcccaa ttccgtgatc tatatggact acataatctc caggtttcag
     2221 atcttcgtaa tttacaattg tttccccagg tgcataacga ggagtgtagc gtttaccctt
     2281 atagcgatta aagatttcgt ggtcggtata taaaatcaag cggcaatctt caatagaaaa
     2341 gccttcgtgc aagaccccga tgtattcggt gaaagggatt tcgggtaatt gttcacttgg
     2401 atttctgttt tcgctggaag gataaatagt ttgctgcatc cgcttagcct ggctttggtt
     2461 atcgaaaagc agaatattat tccagccctt tttttgtttt tccataagag ttttagttag
     2521 aaaccccaag tcggattcaa aattgggttg actttctatc ggggcacgca gattttgggt
     2581 ggggaaggaa agttcaaatt cactttgcga aaggtataaa ttatcgctgc cggattttat
     2641 tttcgtaaga gtatccaaat cggcaaacat cttttctgga agcggaatgc gagcccgacc
     2701 ttgaattttt gcttccttct gccaggtaga aattgcctgc tcaaaaagag tttcatattc
     2761 ctctcggata tagtaataat tattccaaac aagaatacga ttatcctttt caaaataacc
     2821 agcaaatgtt tgtaactctt tacaaagcaa agcgtaataa ttctctatac cttcaaaaaa
     2881 acccttttct cgaattttgg caataatagg agaaccacta ttaatatcac taatcgccag
     2941 ctcgcgcgct ggaagaatag taaattcagt taaatattct tctacacttc gttgcgtagc
     3001 tatagaaaat aaacgcattc caataatttc gtctccccaa aattccaaac gcacaggatg
     3061 aattagcgga ggagaaaaaa catcaataat tccacctctt cttgctgcct gaaaaacttt
     3121 actaacctga tattgaatat cgtatcccat atcatagagc ttatgaagta atacatcagg
     3181 atcatattcc ataccttgtt gcaaatgtaa tatgtgtcta cctaataaat cacgggaagg
     3241 caaaaagcga cccagagctc taatggaaag agaatatata ccgcttgtag aattttgaaa
     3301 tgtggaaagc aatgtctcca tccgcgttgc ccgtatactg taatgtggag aacgttcttc
     3361 atatggtaaa atttcgtaat cgggtaaata ataggcattt tctttgccaa ttaacacaca
     3421 gagatcatcc catatctctt cagcaatgat atcatcctga gaaacaataa taacattttt
     3481 accagtttgt ttccataaat gagctgcaat taatgctctg gcactttgat taagatggta
     3541 aatttggata gacctatctt tcaagggaag cttttgcacc ccttgcataa actcgctttg
     3601 taataattgc tcttctatct tagagtatag cattttatgg tctctcacag attacacgga
     3661 tgtatatttt tttagttctt cattataggt attatgcgcc cttatcacta aatgagttct
     3721 taggcagcat aaatatttct ttggtgatga taattattgg cttagcactc tcactgaacg
     3781 aagttctttc acttagtaac ctccacataa ctaccaataa ttttggctaa tctattacca
     3841 aaggatatag taagtcgagc atcgggtcca tcaccatcta cactcaaaat aactcctata
     3901 ccatattcag aatgatatac tttttggccg attttatagt gtttttggtt ttcagtgatc
     3961 acttttccag ctttcttcat tcgttttggt ggagctgcaa ttccaccgtc gccttgggta
     4021 agataatata attcagaatc tatttctgct aaaaactggc ttcgaaccgg aaaataaaag
     4081 gtatcatata agcgtctggt tttagcatag cttagatgta aataattttt ggctctggta
     4141 attcctacat aaaaaaggcg cctttcttct tcaatatttt cgcgggtgtt catactcatt
     4201 ctatgaggaa ggagctcatc ttccaagcct acaatgaaca ccatttcaaa ttccaaacct
     4261 ttggcattat gtaaggtcat taaccgtaag gaatccactt cttctttcat tctatccata
     4321 tctgtctgta aagcaacata gggcagataa tcttcaatta gagggaggcg gtcattttct
     4381 cgataaaagt gctcactgaa ttcgctaaca gaagtaacaa attccactat gttttcagcg
     4441 cgggcaattt cgcgggggtc gttgctattt ttatatcttt ctattagttc caattcttcc
     4501 acaatggtct ttacaagttc taaagcaggt ttttgggtaa tgctattatg ccatctttcc
     4561 atcagcaagt aaaattcgct gatgcgattt cttgcaggat tctttagttc ttcaacggca
     4621 tcagcatatt ggagagcttt atatagagac atctgagttt tgttagcaaa gctgattaat
     4681 ctgtttatac tggtttggcc aattcctctg gtgggttcgt taattatgcg taatagagat
     4741 tcattatcgt tcacattgct tagcacataa agatatgcca gtaagtcctt tatttctttg
     4801 cgctgataga attgaacccc tccaacaatg ctgtaaggaa tttccgcctg aattaaggca
     4861 ctttcaaaaa ctcgggattg ggcattagtt ctatataaaa cagctatttc gcgtaaagga
     4921 atccccttat tctgtaaatt gacaacttcc tcggttattt tatttgcttc ctcaatctca
     4981 ttttcgtaaa cggcaaggat aggtttctca ccggtgggaa gttcactcca aagttctttg
     5041 ttgtgtcttc tccggttatt ggcaataaca gcattagcca aagctaaaat attccccgta
     5101 ctgcgataat tctgttccag acggatacta tgcacatttg tgtagtccct ttcaaattcc
     5161 agaatatttc gtaaactggc tccccgaaaa ctatatattg cctgatcgtc atctccaact
     5221 acacacactc tttgatgccc tgcagctatt tgatacacga tttcaaattg agccttatta
     5281 gtatcctgat attcatcaat cattacatat tgaaattgag aacggtactt ttcgcggaca
     5341 gcattattgg aaattagaag cttggctgtc cacaacaaaa tatcgtcaaa atccagtgcc
     5401 tgatttagta agagctgttg ttgatataag cggtaaatat ttataaaagt ggcaaattcg
     5461 ctatctttct catcatccgg gttttgtata tcttccggca gcattagttt atttttataa
     5521 cggctgattc gatttaacat acgatttata ggaaacttct gtttatcaaa accatattcc
     5581 ttataaatcc tcttcaatag ggaaacttga gtgtcttcat catatatgga aaaattggaa
     5641 ttaaaaggta aatgagcact ttcgaaacgc aaaatcctac agcataacga atgaaaagtt
     5701 cctacccata aagttcgcat tggaatattt aaaagctttt ctaaacggtc ctgcaattcg
     5761 cgagctgctt tattggtgaa ggttacaact aaaatattcc aggggtgaat atgcttttct
     5821 ttaatcaagt aggcagtcct ataaataagc gaacgcgttt ttcctgaacc agctcctgct
     5881 aatactaaaa ccggatattc agtatcggtt accacttcca gctgtctatt attcaactgg
     5941 gaagaataaa attctggata catcgtttaa ggaactttaa cagaaaaagg ttcagacctt
     6001 ctgccttcca gtttcccctc aggatatact ttataagctg aaacccgata ccaataatat
     6061 cctggctgaa tagaaggatc attagcactg ctatgtgtaa attcgttgat agttactgtc
     6121 cctaccaaag caaatgaaga attataacca atcccgcgat aaacataata accatcagta
     6181 ttataggggc tattggctac ccatctaata gttaccactc gcagatcttg atctgctgt
//
LOCUS       DAORHB010000071         6220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_12648_length_6220_cov_3.663260, whole genome shotgun sequence.
ACCESSION   DAORHB010000071 DAORHB010000000
VERSION     DAORHB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12648_length_6220_cov_3.663260"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKM80_03205"
     CDS             complement(<1..373)
                     /locus_tag="PKM80_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNV92945.1"
                     /translation="MPKQDLKIKGEKTVDVKEIKKKAQLQKAKEPQKTTKEYQKPAAR
                     VIIDDESLRPTNIVPVKWNRWIAFGVFLITLIVYMCTQARTMSFWDSGEYATCISILG
                     VPHPPGNPFYILLGRAIVAIFG"
     gene            complement(348..1334)
                     /locus_tag="PKM80_03210"
     CDS             complement(348..1334)
                     /locus_tag="PKM80_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNV92946.1"
                     /translation="MGEKLRMTIIYAVLIICGLAMIIPFLWMLSTSLMTQSEFNKQES
                     IFIPRVTYYIWDNGTQEQRILLVQEKGENSIIHILNEEDKIIEEYKEVPSSQVKKITK
                     VPYFHWQNYVKAFNKVPFAIYFKNTILVSLFTLLGVLITSPLAAYAFARMEFFGRDFF
                     FYLFLSMMMVPEPIYLVSSYVLLDKISWLDTYQALIVPWCVNIFTIFLFRQHFKSLPQ
                     ELFDAAAIDGCSTFGMLWRIMLPLSKSVIATASIFSLIGSWNSFMWPLVMTNRPELRV
                     LQVGLSYFNQEASTQTTLLMAASTFSIVPILILFFMAQKQIIASYAKAGLKD"
     gene            complement(1346..2278)
                     /locus_tag="PKM80_03215"
     CDS             complement(1346..2278)
                     /locus_tag="PKM80_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNV92947.1"
                     /translation="MKAKWKISPYLYIFPALFLLMAFRLIPIVMSFIISFFDWSIKGT
                     GKFIGLMNYATMLKDEVFWQSMGNTIWLVIIVVPASIILSLLFAVLLNNIKALKGLFR
                     TVYFMPYVTSLVAVSIVWKLMFNEQTGLINTLIGYIGISPQKWLSESRGIFEMLFSGL
                     GINLHGWIGGPSQALFAIIIMTVWKGLGYNTIIYLAGLQNISKVYYEAADIDGASKIK
                     QFFKITLPLVSPTTFYVLIMTTIVTFQTFSQVYLMTDKGGPLNTTKLIVYYIYEKGFD
                     TLEMGYASAVALALFIIILALTIYQKRLEKHVNY"
     gene            complement(2275..3708)
                     /locus_tag="PKM80_03220"
     CDS             complement(2275..3708)
                     /locus_tag="PKM80_03220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV92948.1"
                     /translation="MNETSKPRILIVDDSVETLELFELQLKNKYKVDTATSLKAGKEL
                     LDNYGYHIAIIDLILPGENGLDLIQYVTQSYPHTAVIAISGQASIETAVAAMKLGANE
                     YLVKPFRDLDIINIQVEKILQTQWLIAENKRLSALIDTDIETDLIIGNSPAIQSIMQT
                     VRKIAKLDTSTLITGETGVGKSVFANLIHRNSLRKHQKFVTVNCGSLTETLLESLLFG
                     HKRGAFTDAYRDKIGYFQEANGGTLFLDEITETSLSFQVKLLKVLETGFFRMVGGEED
                     VHTDVRIITATNKDIGECVANGTFREDLYYRLNVFKIHIPPLRERRDDIKVLAYAFTN
                     EFCNKYNKGELKLSPEVMSILLNYSWMGNIRELKNVIEHAVILAEHNVILPEDLPENI
                     VNSTNMISHAFQQEEYGDWLSAKQNFERRYLNQLLSQCNGNFSKASKISGITRENLYN
                     KCAKLGIDYKQFRTRNNLQKETKETTA"
     gene            complement(3705..4280)
                     /locus_tag="PKM80_03225"
     CDS             complement(3705..4280)
                     /locus_tag="PKM80_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92949.1"
                     /translation="MKDLPPAILEGLIHNIRTPLNLILGYVHRLKQQVNCDEAEQIYK
                     AGIKLEDLLQETWEAIQIRGGGNSKTPLNTWLDAELNFLHNYLEIKHRFIFTPHYYEK
                     EILGKITPIALSEWFESFLMFITEHIPEGQISMQIFVNQQGLKIFINGSNYLADISDL
                     NLPPEFFSFNQDIKVDLKYENAKLVTMVKLI"
     gene            complement(4282..5478)
                     /locus_tag="PKM80_03230"
     CDS             complement(4282..5478)
                     /locus_tag="PKM80_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV92950.1"
                     /translation="MELSKRAREIQASPIRKLNPIANATKAKGIKVYHLNIGQPDLHT
                     PPEMLKAYHEFSDKILAYGPSQGLDLYREGLVKYYKRCGIEVKPDDIIVTTAGSEAIT
                     FAMMVVCEVGDEIMVPEPFYTNYNGFATMAGINLVPITTYAEDGFALPDNSTIEAKIT
                     NKTRAIMLCNPGNPTGTVYSNDEIFRIAELAKRRGLFFISDEVYREFIYDGLTHTSVL
                     DVPDFEENAILVDSVSKRYSACGARIGCIVSRNKELMASVLKFAQARLCPPTVDQLAA
                     NAGINLPDSYFEQIRTEYQSRRDLVMSELDKIPGIVCRKPEGAFYINVKLPIENSEDF
                     IIWMLKEFQIDNETVMAAPGDGFYATEGLGKNELRLAYILCQEDLKKAMSIFAKGLAT
                     YQRIHK"
     gene            complement(5701..>6220)
                     /locus_tag="PKM80_03235"
     CDS             complement(5701..>6220)
                     /locus_tag="PKM80_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92951.1"
                     /translation="NYLQNDPPTLPVTFVENLEWERGNALSVYKAKDYINNEPFLLSM
                     SDHLVKMEALKKIVEVPERINLLLTDPFISENFDVDDATKVLTEDDYIIAIGKELDNY
                     NALDCGIFRLEPDFFTAVEKSVQKGNESISNAITELISLKRIKVVMLNKANQWMDIDT
                     PEAYNFAHNFVI"
BASE COUNT         2030 a         1140 c         1031 g         2019 t
ORIGIN      
        1 aaccaaaaat ggcaactatc gctctaccta aaagaatgta aaaaggattt cctgggggat
       61 gaggaacacc taaaatactg atgcaggttg cgtattctcc actatcccaa aaactcatag
      121 ttcgtgcttg agtacacata taaactatta aagtaataag aaatactcca aaagcaatcc
      181 aacgattcca ttttaccgga acaatgtttg taggacgaag tgattcatcg tcaattataa
      241 cacgggcagc aggtttttga tattccttag ttgttttttg aggttctttc gctttttgta
      301 attgcgcttt tttctttatt tcttttacat ccactgtttt ttctccttta atctttaagt
      361 cctgctttgg catagctggc tattatttgt ttttgtgcca taaagaatag tatcaagata
      421 gggacgatgc tgaaagttga agcggccatc aataaagtag tttgagttga tgcttcctga
      481 ttaaaataac taagccctac ttgtaaaact ctcaattctg gtctattagt cataactaat
      541 ggccacataa aactattcca gcttccaatt agcgaaaaaa ttgatgcagt agcgataact
      601 gatttggaaa gagggagcat aattctccag agcataccaa aagtactaca accatcaatt
      661 gcagctgcat caaaaagttc ttgaggcagg gatttgaaat gctgacggaa aagaaagata
      721 gtaaaaatat tcacacacca gggtacaatt agtgcctgat aagtatccag ccaacttatt
      781 ttatccaata agacataaga agatacaaga taaattggct ccggaaccat catcatactt
      841 aaaaatagat agaaaaagaa atcccttccg aaaaattcca tcctggcaaa agcataagct
      901 gccagaggag aagttataag tacacccaat aaggtgaaaa ggcttaccag aatagtattt
      961 ttaaagtaga tggcaaaagg aactttgtta aatgctttca catagttctg ccagtggaaa
     1021 taaggtactt tagttatctt tttaacctgg gagctgggga cttctttata ttcctcaatt
     1081 atcttatctt cttcatttag aatatgaata atgctatttt cacctttttc ctgcactaat
     1141 aagattctct gttcttgtgt tccattatcc caaatataat atgtaaccct aggaataaaa
     1201 atactctcct gtttgttaaa ttccgattga gtcatcaaag aagtggaaag catccacaaa
     1261 aaaggaatta tcatagccaa tccacaaata atcaggacag cataaataat ggtcattcgt
     1321 aatttttcac ccattttcaa cttccttaat agttaacatg tttttccaaa cgcttttgat
     1381 atatggttaa agcaagaatg ataataaata aagctagtgc tacggcactt gcataaccca
     1441 tttctaaagt atcaaaaccc ttttcataaa tataatatac aattagcttg gtagtattta
     1501 agggtccacc cttatccgtc attaaatata cctgcgaaaa ggtttgaaaa gtgacaatag
     1561 tagtcattat caacacataa aaagtagtag gtgaaactaa agggagagta attttgaaga
     1621 attgttttat tttactggct ccatctatat cagctgcttc ataatagacc ttggaaatat
     1681 tttgtaaacc agcaagataa ataattgtat tgtagcccag tcctttccaa acagtcataa
     1741 taattatggc aaaaagggct tgagatggac ccccaatcca accatgaaga ttaataccta
     1801 atccagaaaa cagcatttca aaaatccctc tggattccga taaccatttt tgaggggaaa
     1861 taccaatgta tccaatcaaa gtattaatca agccagtttg ttcattgaac attaatttcc
     1921 aaacaatgga taccgcaacc agtgaagtta cataaggcat aaaatagaca gttctgaaaa
     1981 gacccttcag ggcttttatg ttatttaaaa gaacagcaaa aagcaaagat agtataatac
     2041 ttgcaggaac aacaataata accagccaaa tagtatttcc catagattgc cagaaaactt
     2101 catcttttaa catagttgca taattcatta atccaataaa tttgccagtt ccttttatag
     2161 accaatcgaa aaagctgata ataaagctca taacgattgg tatcaaacga aaagccatta
     2221 acaaaaataa tgcagggaaa atgtagagat agggtgaaat tttccatttc gctttcatgc
     2281 tgtagtttcc tttgtttctt tctgcagatt attacgggtg cgaaattgtt tatagtcaat
     2341 tcccagtttg gcacatttat tatataaatt ttctctggtg atgccactaa tttttgaagc
     2401 tttactgaaa ttgccattac attgggaaag caattgattt aaataacgac gctcaaaatt
     2461 ctgcttggca gataaccaat caccatattc ttcttgctgg aaagcatgcg aaatcatatt
     2521 tgttgaatta acaatatttt ccggtagatc ttcaggtaaa ataacattat gctcggctaa
     2581 aataacagca tgctcaataa catttttcag ctctcgtatg tttcccatcc aggaataatt
     2641 caataaaata gacattactt caggagagag ttttagctcc cccttattat atttattaca
     2701 aaattcatta gtgaaggcat aagcgagcac tttaatatcg tctctgcgtt ctcttaaagg
     2761 aggaatatgg attttaaaaa catttaagcg ataatataaa tcttctctaa aggtgccatt
     2821 ggcaacgcat tccccaatat ctttgtttgt agctgtaata attcttacat ctgtatgaac
     2881 atcttcttca ccaccaacca tacggaaaaa acctgtttcc agaaccttca gtaatttaac
     2941 ctgaaaggaa agtgatgtct ctgttatttc atccagaaaa agagttccgc cattagcttc
     3001 ctgaaaatat ccaattttat cgcgataagc atctgtaaaa gcacctcgct tatgaccaaa
     3061 aagtaaactt tccaatagag tttcggttaa actgccacag ttcactgtta cgaatttttg
     3121 gtgtttacgc aagctgttac gatgaattaa attggcgaaa acactttttc ctactcccgt
     3181 ttctcctgtt attaaagtgg aagtatccag tttggcaatt tttctcactg tttgcataat
     3241 tgattgaatt gcaggagaat tacctattat caaatctgtt tcaatatcag tatcaattaa
     3301 agcgctaagc cgtttatttt cagctattag ccattgagtt tgtagtattt tttctacttg
     3361 gatattgata atatcaagat cacgaaaagg ttttaccaga tattcattgg ctcctaattt
     3421 catagcagca acagctgttt caattgatgc ttgaccactg atagcaataa cagctgtgtg
     3481 aggataactt tgagtaacat attgaatcag atcaagtcca ttttctcctg gaaggataag
     3541 gtctatgata gcaatatgat aaccgtaatt atccagaagc tctttccctg cttttaaact
     3601 tgtagcggta tccaccttgt atttattctt gagctgaagc tcaaaaagct ccaaggtctc
     3661 aacgctatcg tcaacaatta gaattctggg tttacttgtt tcattcatat caattttacc
     3721 atagtaacca gtttggcatt ttcatatttc aagtcaacct taatatcttg attgaaactg
     3781 aaaaattcgg gaggaaggtt caaatctgat atatcagcaa gataattgct tccattaata
     3841 aagattttta gcccctgctg atttacaaat atttgcattg atatttggcc ttcaggtata
     3901 tgttcagtga taaacatcaa aaaagattca aaccattcac ttaaagcgat aggtgtaatt
     3961 ttccctaaga tctctttttc gtaataatgc ggagtgaaga taaagcggtg tttgatctcc
     4021 aggtagttat gcaaaaagtt aagttctgca tctagccagg tattaagagg tgtttttgaa
     4081 tttccaccac cacgaatttg aatcgcctcc caagtttctt gaaggaggtc ttctaatttt
     4141 ataccggcct tataaatctg ttctgcttca tcacaattaa cttgctgttt gaggcgatga
     4201 acataaccta aaatcaggtt taagggagtt cggatattat gaattagtcc ttcaagaata
     4261 gcgggaggca aatctttcat cttatttatg aatcctttgg tatgtagcaa gccctttggc
     4321 aaagatactc attgcttttt tcagatcttc ttggcaaagg atatatgcta agcgaagttc
     4381 atttttccca agcccttcag ttgcataaaa accgtctcca ggagctgcca taactgtttc
     4441 attatcaatt tgaaattcct taagcatcca aatgatgaaa tcctcagaat tttcaatggg
     4501 tagctttaca ttaatataaa aagctccttc aggtttccgg caaacaatac cggggatttt
     4561 atccagttca ctcataacca gatctcgacg agattgatat tcagttctta tttgttcaaa
     4621 atagctatca ggtaaattaa tacctgcatt ggcagctaat tgatcaaccg taggaggaca
     4681 aagtcgtgct tgggcgaatt ttaaaacgga agccattaat tccttatttc tactaacaat
     4741 acaaccaatt cttgcaccac aggcactata gcgtttagaa acactatcca ctaatatggc
     4801 attttcttca aaatctggaa catctaaaac acttgtatga gttaaaccat cataaataaa
     4861 ttcacgatag acctcgtcag atatgaaaaa gagacctctg cgttttgcta attcggcaat
     4921 gcggaatatt tcatcgttac tatatacagt tcctgtagga tttccaggat tgcaaagcat
     4981 aatggctcta gttttatttg ttattttggc ttcaatagta gaattatcag gaagagcaaa
     5041 accatcttct gcgtaagtag tgatgggaac caaatttatt ccagccattg tagcaaaacc
     5101 gttataattg gtataaaatg gctcgggaac cattatttca tctccaactt cacaaacaac
     5161 catcatcgca aaagtaattg cttcacttcc tgcagttgta acaataatat catctggctt
     5221 aacttcaatt ccacaacgct tataatattt aactaagccc tctctataaa gatcaagacc
     5281 ttgcgatgga ccgtaagcta agatcttatc tgaaaattcg tgatacgcct ttagcatttc
     5341 tggaggagta tgtagatcag gttgaccaat atttaagtga taaactttaa ttccttttgc
     5401 tttagtggca ttggctatgg gattgagttt tcgaatagga gatgcttgta tctcccttgc
     5461 tcttttggat agttccattt tttgcatcct tattattata tttttcttct gattccttta
     5521 tttaaaagag cagaaaaaag acaaggaaaa ttttgttacg ggagaggatt tatcaagcta
     5581 ttcttttgtc acaaggtggg ttgaagtcct cgttaataac agctataagt tcataaccta
     5641 ctgtataaga taaaggtata agaagttttg cagagaaacg atcagatctc gtttttcctg
     5701 ttatataaca aagttatggg cgaaattata tgcttcggga gtatctatat ccatccactg
     5761 atttgcttta tttagcatca ccactttaat cctttttaaa gagatcaatt cagttatcgc
     5821 gttactgata gattcattac ctttttgcac tgatttttct actgcagtaa agaaatcagg
     5881 ttccaaacgg aatatgccac aatcgagagc attataatta tcaagttctt ttccaatagc
     5941 gattatataa tcatcttcgg taagtacttt tgtagcatcg tctacatcaa agttttcgga
     6001 gataaaggga tccgttagca gtaaattaat tctttcagga acttccacta tttttttaag
     6061 ggcttccatt tttaccaggt gatcagacat cgaaagaaga aagggttcgt tattaatata
     6121 gtcctttgct ttataaactg aaagagcatt accgcgttcc cattcaaggt tttccacaaa
     6181 agtaacaggt aaggtaggtg gatcattttg caaatagtta
//
LOCUS       DAORHB010000072         6134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_12898_length_6134_cov_6.450732, whole genome shotgun sequence.
ACCESSION   DAORHB010000072 DAORHB010000000
VERSION     DAORHB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12898_length_6134_cov_6.450732"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..2604)
                     /locus_tag="PKM80_03240"
     CDS             complement(181..2604)
                     /locus_tag="PKM80_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNV92952.1"
                     /translation="MKVDIEHLLPLVEKPSRYIDHEINACRKDFSAFPIHFAFAFPDT
                     YELGFSHLGIKILYSIINQLDYAMADRVYLPWIDLIEKMQDEGLELFGWESRRQIKDF
                     DILGITLQSELTFTNVLELINLSGIEIHRDKRKETDPIILAGGPCATNPLPLAPFIDV
                     FFIGEAEDGIIEIAEILKTESNRTERIKQMAQLKSCYVPSIHNGTELIQSRKYINFPS
                     SEAQHTPQLLPWQLATHNRLTSEIMRGCTRGCRFCFAGYFYRPVREREPQTIIENILK
                     EIKESGWDEVGLLSLSSSDYSCIQNLLQQLLENLDTQKTHISLPSLRVDTLDNAIVQS
                     LKNLGREGLTIAPEAGSQRLRQVINKNLSEEQILAGIETALKLGWQKIKLYFMLGLPT
                     ETEEDIEAIIALIQKINNAGNRHLQINVTLSPFVPKPFTPFQWCAMQDFDTLLKNAIR
                     IKNAFARAKNIKIHYHTIENSLLEALLSRGDIKIAELIETAWKKGAHFDGWKECFNFS
                     LWQEALTECSITLDNYLGERNLQDILPWDFISLGIDKSFLISEWEKAKTGIQTKDCRE
                     ICSSCGVCKDEVQTIIAQGTFLKEGTSSTEVKKTFLQNRQYKYRVFYQKKGLLRFIAH
                     LDWMRMLFRRISLIDLQTVFTQGFSPHPKVSLCPPLAVGIEGLAEYFDLAFYQQYSPE
                     EILREFQQTPILDFTVTAVQPLNKDEKQPTSEVISVFLADDLLSQVRTKIDFFAQSKT
                     FSYTKSRPAKDKTYDLKKIILAMNLQGNELHIEKLLESPSLFDILAAILEMDKTILYQ
                     QHIYRIAFK"
     gene            complement(2601..3314)
                     /locus_tag="PKM80_03245"
     CDS             complement(2601..3314)
                     /locus_tag="PKM80_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNV92953.1"
                     /translation="MKIGGLQKFSLLDYPGELSAIIFTQGCNFRCPYCHNPELVDPVR
                     YSPLLDTEWVLRFLYKRHKKLSAVVVTGGEPTLQEDLIPFLKLIKAMRYKIKLDTNGS
                     HPDVLQEIGNLGLVDYWAMDIKAPIKLYKVITRADLESGIIEKSMDIIRQSGIDYEFR
                     TTYFDLLLTSSDLASMQELLKPGDRFIIQQCRYENTLQEITREELLTPALSFNENPAC
                     RSLINWGTEHSVKISLRSL"
     gene            complement(3314..5449)
                     /locus_tag="PKM80_03250"
     CDS             complement(3314..5449)
                     /locus_tag="PKM80_03250"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNV92954.1"
                     /translation="MFSSIRKRNGSIVVFDQQKIAKAIESAGFATGEFGQDIAAVLTL
                     RVLNLAQQVIQDEIPEVEKIQDIVEEVLLASPYKKTAKAYIIYRDQHSQIRNLVSKAG
                     VDLIDQYLDRLDWQVNENSNMAYSLQGLNNYIASEVSKTYWLNKIYPPEIREAHLSGD
                     LHIHDLGQLSVYCVGWDLMDLLLTGFCGAEGKVESSPAKHFRSALGQIVNFFYTLQGE
                     AAGAQAFSSFDTLLAPFIFYDGLNYKEVKQALQEFIFNINVPTRVGFQTPFTNITLDL
                     KPHQLYKDQAVIIGGKPMNKTYGDFQAEMDILNRAFLEVMIEGDAKGRVFTFPIPTYN
                     ITKDFNWDDPNLDYLWEATAKYGIPYFSNFVNSDMDPKDARSMCCRLRLDTRKLETRG
                     GGLFGANPLTGSIGVVTINLPRIGYLAKTEEEFFALLEERLQLAKKSLEIKRKVLESF
                     TQSGLYPYTKFYLKSIYERFGQYWKNHFSTIGIIGMNEAMLNFMDENIGASKAKDFAL
                     KIMDYLRNRLLEFQEETENNYNLEATPAEGTSYRLALLDKKHFPEITCANCDDKAPFY
                     TNSTQLPVNYSDDIFEVLDLQDELQTKYTGGTVLHIFGGERVEYGQSIKALVKTICEG
                     YRLPYFTFSPTFSICPTHGYLDGEQPICPLCQNRTEVFSRIVGYLRPVSQWNEGKKAE
                     FKMRTTFDLHKQPKKDSLILPETVSAKSV"
     assembly_gap    5969..6068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1852 a         1166 c         1113 g         1903 t
ORIGIN      
        1 tgacttcggt gttctcggtg gcttattata aaagaatccc ttaaatcctt ataatcctgt
       61 tcatcccaga cctattattt tctctttttg cggaggaacg gcgtcctccg gctacacatt
      121 tttagctata agcaagatac cataaagaaa aaagttttgc aggcacggat ggcttcttac
      181 tcacttaaag gcaattctat atatatgttg ttgatagagg atagttttgt ccatttccaa
      241 aatagcagcc aaaatatcaa aaagggaggg actttccaac aatttttcta tatgtaattc
      301 gttaccttgc aggttcatag ctaaaataat cttcttcaaa tcgtaagttt tatcttttgc
      361 aggtctgctt ttcgtatagg aaaatgtttt gctttgggca aagaaatcta tttttgttct
      421 aacctggcta agtaaatcat ccgctaaaaa aacactgata acttcgctag ttggttgctt
      481 ctcatcttta ttcagaggtt gaacagcagt tacagtaaaa tctaaaatag gggtttgctg
      541 aaattcccgc aaaatttctt ccggcgaata ttgttgataa aaggcaagat caaaatattc
      601 tgccaaaccc tcaataccaa cagccaaagg agggcagagc gacaccttag ggtgagggct
      661 aaaaccctgg gtaaaaacag tttgcaggtc aattagagaa atgcggcgga aaagcattcg
      721 catccaatcc agatgggcaa taaagcgtaa aagtcctttc ttttgataaa aaacccgata
      781 tttgtattgt ctgttttgta ggaatgtttt ttttacctct gtggaagatg taccctcctt
      841 taaaaatgtt ccctgggcaa ttattgtctg aacttcgtct ttacataccc cacaggaaga
      901 acaaatttct ctgcaatcct tggtttgaat acctgttttt gctttttccc attctgaaat
      961 taaaaaactt ttatctatgc ccaatgatat aaaatcccaa ggcaaaatat cttgtagatt
     1021 gcgctctccc aagtagttat ctaaagtaat actacattca gttaacgcct cttgccacaa
     1081 ggaaaaatta aagcactctt tccagccatc aaaatgagct ccttttttcc aggcggtttc
     1141 aataagttct gctattttta tatctccccg actaagcaat gcttctaaaa gggaattttc
     1201 tatagtatga tagtggattt tgatgttttt agctctggca aaggcatttt ttattcgtat
     1261 cgcatttttt agcagagtat caaaatcctg catcgcgcac cattgaaaag gtgtgaatgg
     1321 ttttggtacg aaaggcgaaa gggttacatt tatttgcaga tgcctatttc cagcattatt
     1381 tattttttga ataagagcaa taatggcttc aatatcttct tccgtttcgg taggaagccc
     1441 caacataaaa tatagcttaa ttttttgcca gccaagtttt agtgcggttt caatgccagc
     1501 aaggatttgt tcttcgctta ggtttttgtt tatcacttgg cgtaaacgct gtgaaccagc
     1561 ctcgggagca atggttaaac cctctctacc caaattttta agggattgaa caatagcgtt
     1621 atccaaagta tccacacgca aagaaggcaa agaaatatgg gttttttgag tatccagatt
     1681 ttctaaaagt tgctgcagca gattttgaat gcaggaataa tcgctactgg aaagagataa
     1741 cagaccaact tcatcccacc ccgattcttt tatttctttt aaaatgtttt ctataatagt
     1801 ttggggctcg cgttcccgga ccggacgata aaaatacccg gcaaaacaaa atctacaacc
     1861 ccgagtgcaa cctctcataa tttcggaagt taagcgatta tgagttgcca gctgccaagg
     1921 taaaagttgg ggagtatgtt gtgcttcaga agaaggaaaa ttaatatatt tacgactctg
     1981 gatgagctcg gttccattat gaatagaagg aacataacaa gattttagct gtgccatttg
     2041 tttgatgcgt tcagtgcggt tactttcagt tttcaaaatt tctgctattt caatgattcc
     2101 gtcttccgct tcgccaataa aaaacacatc aataaaagga gccaagggca aaggatttgt
     2161 ggcacagggt cctccggcaa ggataatggg gtctgtttct ttgcgtttgt ctctatgtat
     2221 ctcaataccg cttaagttta ttagctccaa tacattagta aaagtaagtt cactttggag
     2281 agtaatccct aatatgtcaa aatccttaat ttgtcttcta ctttcccaac cgaaaagttc
     2341 caaaccctca tcctgcattt tttcaattaa atcaatccaa ggtagataaa ccctatccgc
     2401 catagcgtaa tccaactgat ttataatgga atagagtatc tttatgccca ggtgggaaaa
     2461 acccaattcg taggtatccg gaaaagcaaa agcaaagtga attggaaaag cagaaaaatc
     2521 cttgcggcag gcatttattt cgtgatcaat atacctggaa ggtttttcca ccaaaggcag
     2581 aaggtgttct atatcaactt tcataaactg cgcaaagata ttttcacgct atgttctgtt
     2641 ccccaattaa ttagagagcg acaagcagga ttttcattaa aagaaagggc aggggtaagc
     2701 aattcttctc tggtaatttc ttgtagagtg ttttcatagc gacattgttg aatgataaat
     2761 ctatcccccg gttttagcaa ttcttgcata gaagcaagat ctgaagaagt taatagcaaa
     2821 tcaaaatagg tagtgcggaa ttcgtaatca attccggatt ggcgaataat atccatactt
     2881 ttttcgatta ttccagattc taaatctgct ctagtaataa ctttatatag ttttataggt
     2941 gcttttatat ccattgccca ataatctacc aaacccagat taccaatttc ctgcaaaaca
     3001 tcagggtgag agccattggt atccagtttt atcttataac gcattgcctt aattaatttt
     3061 aagaatggaa taagatcttc ttgcagagtt ggctcccctc cggtaactac tactgcggat
     3121 agttttttgt gccgtttata cagaaagcgt aatacccatt cagtatccag taatggacta
     3181 tatcttacag gatcaaccag ttcgggattg tgacaatagg ggcagcgaaa attgcatccc
     3241 tgcgtaaaaa ttattgctga taactcacct ggataatcca acaatgaaaa cttttgcaaa
     3301 ccaccaattt tcattatacg ctctttgctg aaacggtttc tggtagaatt agactatcct
     3361 ttttcggctg tttatgaagg tcaaaagtag tgcgcatttt aaattctgct tttttacctt
     3421 cgttccattg ggaaacgggt cgcaaataac ctacaattct ggaaaagact tctgtcctat
     3481 tttggcagag aggacaaatt ggctgttcgc catccagata gccatgagtt ggacaaatac
     3541 tgaaggttgg agaaaaagtg aaatagggca agcgataacc ctcacagata gtttttacca
     3601 gtgcttttat actttggcca tattcaactc tttctccgcc aaagatatgt aaaactgttc
     3661 cacctgtata tttggtttgg agttcatcct gcaaatccaa aacctcgaaa atatcatcag
     3721 aatagttaac gggcagctga gtgctatttg tataaaaagg cgctttgtca tcacaattgg
     3781 cacaagttat ttctgggaaa tgttttttat ccaaaagtgc taaacgatag cttgttcctt
     3841 cggcaggagt tgcttccaag ttataattat tttctgtttc ttcctgaaat tccagtaaac
     3901 ggttgcgtag ataatccata attttcaggg caaagtcctt agcttttgaa gctcctatat
     3961 tttcatccat aaagtttagc atcgcttcgt tcattccgat aattccgatg gtggaaaaat
     4021 gatttttcca gtattggcca aagcgttcat aaatgctttt taaatagaac tttgtatagg
     4081 gataaagtcc gctttgcgta aaggattcta aaactttgcg tttaatttcc aacgactttt
     4141 ttgctagttg taaacgctct tccaagagag caaaaaattc ctcttccgtt tttgctagat
     4201 aacctatgcg gggaagattt atggttacca ctccaatgga accggttaaa ggattggcac
     4261 caaaaagtcc accccctctg gtttccagct tacgggtatc caaacgtaaa cgacaacaca
     4321 tactgcgtgc atctttagga tccatatcgg aatttacaaa attggaaaaa tagggaatgc
     4381 catatttggc tgttgcctcc cataagtaat ccagattagg atcatcccag ttaaaatctt
     4441 ttgtgatatt ataggttgga atgggaaaag taaaaacgcg tcccttggca tcaccttcaa
     4501 tcatcacttc caaaaaagct ctattcaaaa tgtccatttc tgcctgaaaa tcaccatagg
     4561 ttttattcat cggtttgcca cctatgataa ctgcctgatc tttatataat tgatgtggct
     4621 ttaagtctaa agttatatta gtaaatgggg tctgaaaacc aactcgcgta ggcacattga
     4681 tattaaagat gaattcctgc agcgcttgtt tcacttcttt atagttaagc ccatcataaa
     4741 aaataaaggg tgccaaaagt gtatcaaaac tggaaaatgc ttgtgctcct gcagcttcac
     4801 cttgcaaagt ataaaagaaa ttaacaatct gtcctaaggc gcttctaaaa tgtttggcag
     4861 gagaactttc cactttgcct tctgcaccgc aaaaaccggt taaaagtaaa tccatcaaat
     4921 cccagcccac acaataaact gaaagctgac ccaaatcgtg aatatgtaaa tcgccactta
     4981 aatgtgcttc ccgaatttca ggaggataga tcttattcaa ccagtatgtc ttactaactt
     5041 cagaagcaat ataattgttc aagccctgaa gtgagtatgc catattggaa ttttcgttta
     5101 cctgccaatc taatctatcc agatattgat ctatcaaatc cactcccgct ttggaaacca
     5161 agttgcgaat ctgggaatgc tgatcacgat agataatata tgccttggcg gtttttttgt
     5221 aaggagaggc aagcaaaact tcctctacaa tatcttgaat cttttccacc tcgggaattt
     5281 catcctgaat aacttgctgg gctaaattga gcacgcgtaa agttaaaacc gcagcaatat
     5341 cctgtccaaa ttcacctgtg gcaaatccag cactttcaat ggccttggct attttttgtt
     5401 gatcaaaaac aacgatggaa ccgtttcttt tgcgtataga tgaaaacatc tcaaaacctc
     5461 tattttataa atttaatatc ttatttagat ataataagtt agaatgctga gaccgtgttt
     5521 tgggaaatcg ggtctcagca accgaagttt atggaaatat ggagttacaa tttgtcaagc
     5581 ggaattgtga tttttcccgc agatttagtt tcacgcagat tacgcagatt acgcagattt
     5641 ttttagaaac gaggatttta ggattttagg attaagagga ttttttatat ttagccaccg
     5701 agatcaccga tattaactaa gccaccgaga acaccgaaga caccgaaaaa aatatcttct
     5761 cacagattac gcagattacg cagatttttt tagaaacgag aatttaagga ttttaggatt
     5821 aagaggattt tttatattta gccaccgaga acaccgaaag aaatatcttc tcacagatta
     5881 cgcagattac gcagattttt tttagaaacg aggatttaag gattttagga ttaagaggat
     5941 tttttatatt tagccaccga gaacaccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnac gaggatttaa ggattttagg attaagagga ttttttatat ttagccaccg
     6121 agatcaccga tatt
//
LOCUS       DAORHB010000073         6097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_13024_length_6097_cov_5.524495, whole genome shotgun sequence.
ACCESSION   DAORHB010000073 DAORHB010000000
VERSION     DAORHB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13024_length_6097_cov_5.524495"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="PKM80_03255"
     CDS             complement(<1..1364)
                     /locus_tag="PKM80_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNV92955.1"
                     /translation="MKHFLLILILVSFTLLGAQNTLWVNGSNEAQFIYRTVDDSLHTY
                     FKDAFGFNLGYRNFSFGMKFIAELPKYSTEQTELMDELDANRLELGWQELYASYEKDA
                     CKIYAGITEESFGNGIVFRSYKDLEFDIDNRLESFLFSYNDDFKFKAIYGAIENPNII
                     GRYDLAYGADLQTPYFKGLSFGASAMAFRNLLATNIYNQRTVFAGRLNYSAHNLDLQA
                     ETAFSELYHQPGIGTKNGKAIYVNGSYLIGFITIGGAYKQYDKFQYRLNDLPMANYHN
                     ETLSDASASGEDEIGYQGFGTVNFTDNLNFTVDYAEAFNSDKDKKMNDAYFALEYLGN
                     SYSLLASYSQIEKIDDINSAWQQELIPALQSSFYLFNIPVKIQAEYKKISKQKQITES
                     EHYEPKLQTDFALGKLALSVCVQSSWEEISEMLDSRYYPSAEIKYPLFEHSDIILFGG
                     KEAGG"
     gene            complement(2366..2857)
                     /locus_tag="PKM80_03260"
     CDS             complement(2366..2857)
                     /locus_tag="PKM80_03260"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV92956.1"
                     /translation="MKKITTILFMLLLVSFALAEVMPDFKLPDAAGKNITLQELLGKG
                     PVLIDFWADYCQPCKDAMPQLNTLAEKYDSLTVVMISIDPPKNLPKAKNYLKSKNYKF
                     ITLFDSEKILAKKLGVVNPPHTFILNKTGEIVYSHIGYEPGVENEYELNIRNLLGLQN
                     EAE"
     gene            complement(2869..3978)
                     /locus_tag="PKM80_03265"
     CDS             complement(2869..3978)
                     /locus_tag="PKM80_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92957.1"
                     /translation="MMKKIVYAIMLLALLGIFSCDRFDHSFKPLEQVDFGTELFVPLQ
                     EAYNNISASDLTPVMAFYAEDYNHYGVNKSQWETMLHNMLSEISDPVIEVTLVKASLQ
                     NETNALANWRLKISDSNKAIIADSLYVGERLAKVNGKWLLRGNQCGCTPEVPTQKVIV
                     EYVTNVGCSYCPAVEELLHNLSSEFGDNFIYLTHQLSGPVAINDPLYVYYSAFSAPVS
                     IIQGKHKLASGTQEILNQYYPLVQTELAISNPMDYNIMSALVTGNSISGSVKLTSTTD
                     NFSQEELILNLVIIDRVSTANNAVGEPLTNVVIGRKRIDISSADLNNPITFDFTANVD
                     IPDDAALVVFAQKTPTTFANNATIYSGLQYPLTVR"
     gene            complement(3997..5826)
                     /locus_tag="PKM80_03270"
     CDS             complement(3997..5826)
                     /locus_tag="PKM80_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNV92958.1"
                     /translation="MNRGYKLLLALCLIMLIQPFFAQTVKFLLSPDVLKPGEKGVIKA
                     TLTIPEGKKQTVNPKNPEYFYLEASHPDLSFGKVEYPKPTNIISDEEWNYHPKVTLTL
                     PFTVKQTAKAGNKQVEVLLSYNLCYESGMCDPPEEATKKLSFQIITAEAAEGVVSEET
                     EATENIEKGETSPVSEVDRAATEPIAKRSLGEILKYILFAFLGGIILNITPCVLPILP
                     IRIMSIMNQAQKDRTKVLHHILVYTFGVLISFTIMAVIFIALQKAGESVGWGLQNQNP
                     GFVVTLMAIVFVFALSLLGVFEITVPGMNTANKATSKGGYSGSFFGGIFAFLMAISCT
                     GPFLGAALPFALALPSVLMLIFFLTIGLGFAFPFLLIGIFPKALKIIPKPGNWMIIFK
                     EVMGFVLLFIVYTQLKTLLLLTDGNYLLNVLWFLIILGFASWLYGRFVRVENSKLIQW
                     LFTLISIALVIFAAITYLPIPKADKAEVQVQSGELIPAPNAPEGWYVFSEDILNKALK
                     ENRAVFLDIGAAWCKNCMTNEKTVLFTDTMMQEFKQKNVLLLRGDFTKKDETLLSWIK
                     KHNRAGVPFNALYIPGEEPYIFGELLSKDEVIDALKKIQAKTE"
     gene            complement(5857..>6097)
                     /locus_tag="PKM80_03275"
     CDS             complement(5857..>6097)
                     /locus_tag="PKM80_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92959.1"
                     /translation="KILKSLFRKSILRKLFSVASVAKYSSKILVSKTQSSKSLKSCSS
                     HTYSICEICVICVRHSIISHPIIKQFVWNKFCINL"
BASE COUNT         1886 a         1207 c         1064 g         1940 t
ORIGIN      
        1 ccgccagctt cttttccgcc aaaaagtatt atatcgctat gttcaaaaag aggatatttt
       61 atttctgcgg aaggataata gcggctatcc agcatttcgc ttatttcttc ccaactgctt
      121 tgcacacaaa cagatagagc gagtttacct aaggcgaagt cagtttgcag tttaggttca
      181 tagtgttcac tttctgttat ctgtttttgt ttggagatct ttttatattc tgcttgaatc
      241 ttcacaggga tattaaacag ataaaaactt gattgcagag cagggattaa ttcctgctgc
      301 caagcagagt taatatcatc gattttttca atttgactat atgaggcgag caacgaatat
      361 gaatttccca ggtattccaa ggcaaaatag gcatcgttca tttttttatc tttatcgcta
      421 ttgaacgctt cagcataatc tacagtgaaa ttaagattat cagtaaaatt aacagttcca
      481 aaaccctgat agccaatttc gtcttcccca gaagccgagg catcggaaag ggtttcatta
      541 tgataatttg ccataggaag atcatttaaa cgatattgaa atttatcata ctgtttataa
      601 gctcctccta ttgtaataaa accgatcaaa taactgccat tcacatatat tgccttgccg
      661 ttcttagttc cgattccggg ttgatggtat aattcgctga aagccgtttc tgcttgtaaa
      721 tcaagattat gggcagaata attcagtctt ccggcaaaga cggttcgctg attatagata
      781 ttggtagcca gcagattacg aaaagccata gcggaagcgc caaaagatag acccttgaaa
      841 taaggagttt gcaaatcagc tccgtaagct agatcatatc tacctatgat attaggattt
      901 tcgatagctc cgtaaatcgc tttaaactta aaatcatcat tatagctgaa taaaaaactc
      961 tctaagcgat tgtctatatc aaattctaag tctttatagc tacggaaaac aattccatta
     1021 ccaaaactct cttctgtgat tcctgcgtat attttacagg catctttttc gtaggatgca
     1081 tataattcct gccaaccaag ctccaaacga tttgcatcca gttcatccat caattctgtc
     1141 tgttcagttg aatatttggg tagttcagca atgaatttca tcccgaaact gaagtttcta
     1201 tagcctaggt tgaagccgaa agcatcttta aaataagtat gcaacgaatc atctactgtg
     1261 cgatagataa attgagcttc attggagcca tttacccaga gggtattttg agcgcctaac
     1321 aaggtaaaac tgactaagat caggattaat aaaaagtgtt tcattctccc ttccattgtt
     1381 tatatttttc tctcacagat ttcactgatt acacagatat agatttagaa atatggattt
     1441 caagaatttt tagttattga tgatattgtt aacaataact aattaacttg aacatccatt
     1501 cctttcatct gggtagtcgg agctcgctga agctccgcgt ttttttttgc ggaggaacgg
     1561 cgtcctccgt ctacacatct aaatcctcta aatccttaaa atccttgttt cgaaaaccat
     1621 atctgtcgtc ccgctgggac tattttttat tcgttcttct tgtcgccggg ttaaaacccg
     1681 tcgttagtat gtatcgttcc tagcggaact ttaaattaga aatcctcaaa atccttaaaa
     1741 tcctgttcat cccataccta ttatcatttt cgaggggctt acgccaccgt cgctatcctt
     1801 gtgtcgccct ttgagttaaa atcggctttc catgtgatct gccacctcat ttcattcttt
     1861 tttcctatct ttgaataagg tcattaataa tctcatagaa aaggccgatt ttgacttaaa
     1921 gccgactaat cgtcatttat aaaatacaat cctcttaatc ctataatcct aaaatccttg
     1981 tttctaaatt ttaatccttt gaaacctgtt catcccatac ctattatcat tttcgagggg
     2041 cttacgccac cgtcgctatc cttgtgtcgt cccgctggga ctatttttta ttcgttcttc
     2101 ttgtcgccgg gttaaaaccc gtcgttagta tgtatcgttc ctaacggaac tttaaattcg
     2161 aaatcctcta aatcctaaaa tcctaaaatc cttgtttcta gagctcaaat ctgcgtaaac
     2221 tattttcggt gacttcggtg acttcggtgg ctaaatattc ttcttaaatc ctcgtttcta
     2281 aaactcaatc ctctaaatcc taaaaatcct gttcatccca tacctattcc atctgcgaaa
     2341 tctgcgaaat ctgcgtgaaa atatactatt ccgcttcgtt ttgaagccca agcaaattac
     2401 ggatattcaa ttcatattca ttttctacac cgggctcata gccaatatgg gaataaacaa
     2461 tttctccggt tttatttaga ataaaggtat gcggaggatt caccactccc aattttttgg
     2521 ctaatatctt ttcggaatca aacagagtga taaacttata gtttttgctt ttcaggtagt
     2581 tttttgcttt tggtaaattt ttggggggat caatagagat cattacaacg gtaagcgaat
     2641 catacttttc tgctaaagta ttcagctgag gcattgcatc tttacagggt tggcagtagt
     2701 ccgcccaaaa atcaataagg acgggaccct ttcccaaaag ttcttgtagg gtaatatttt
     2761 ttccagctgc atcgggaagc ttgaaatctg gcataacttc tgccagtgca aaagaaacca
     2821 aaagcagcat aaagaggata gtagtaattt ttttcattcg ctatgctcct atctaactgt
     2881 taacggatat tgtaagccac tataaattgt ggcgttattg gcaaaggtgg taggtgtttt
     2941 ttgggcaaaa acaactaacg ctgcatcatc tggaatatca acattggcag taaaatcaaa
     3001 ggtaataggg ttatttaagt ctgctgaact gatatctatg cgttttctgc ctataacaac
     3061 attggtaagt ggttcaccaa cagcattatt ggcagtagaa acacgatcta taattaccag
     3121 atttaaaatg agttcttcct ggctgaaatt atctgtagtg gaggtaagct ttacgcttcc
     3181 tgagatcgaa tttcctgtta ccaaagcact cattatatta taatccatcg gattggaaat
     3241 agcaagttca gtttgtacga ggggataata ttggtttaga atttcttgcg ttccgctggc
     3301 taatttgtgt tttccttgaa ttatggaaac aggagcgctg aaagcactgt aataaacata
     3361 aagtggatcg ttaatagcaa ccggacccga tagctgatgc gttaaataga taaagttatc
     3421 tccaaattca gagcttaaat tatgcagaag ttcttccaca gcagggcagt aactacatcc
     3481 tacattagta acatattcta ctattacttt ttgggtagga acctcagggg tgcatccaca
     3541 ttgatttccg cgtaaaagcc attttccgtt tactttggct aaacgctctc ctacatataa
     3601 actatcggca ataatagctt tattgctatc gctaattttt aatcgccagt ttgccaaagc
     3661 attggtttca ttttgtagag aggcttttac caaagtaact tctataacgg gatcgctaat
     3721 ttcagaaagc atattatgca gcattgtttc ccactggctt ttattaactc catagtgatt
     3781 ataatcctct gcataaaaag ccatcacggg agttaaatct gaggcgctga tattgttata
     3841 tgcttcttgc agaggaacaa aaagttctgt tccaaaatct acctgctcga gaggtttaaa
     3901 agaatgatca aagcgatcac aggagaaaat tcctaagagc gctaagagca taatagcata
     3961 aacaattttc ttcatcataa tccttcttgt cttatcctat tctgtttttg cctgaatctt
     4021 ttttagagca tcaataactt catctttgga aagcagctcg ccaaatatat atggttcttc
     4081 accgggaatg tataaagcat tgaaaggaac accagctcta ttatgttttt ttatccaaga
     4141 aagcagggtt tcatcctttt tagtgaaatc acctcgtagc agtaaaacat ttttctgctt
     4201 aaattcctgc atcatcgtat ctgtaaatag gactgtcttt tcattcgtca tacaattttt
     4261 acaccaggct gctccgatgt caagaaaaac agctctattt tcttttagtg ccttatttaa
     4321 aatatcttca ctaaacacat accaaccttc tggagcatta ggagcaggaa taagttcacc
     4381 actttgaacc tgtacttctg ccttatctgc tttagggata ggcaaataag tgattgcggc
     4441 aaaaataact aacgcaatcg agatgagagt aaacaaccac tggataagct tactgttctc
     4501 cactcttaca aacctgccat aaagccaact ggcaaaacca agaataatta aaaaccataa
     4561 aacattcagt agataattac catcagttag caacaaaagg gtttttaact gggtgtaaac
     4621 aataaagagg agaacaaaac ccatcacctc tttgaatata atcatccagt ttccaggctt
     4681 ggggataatt ttcagtgcct tggggaaaat accaatcaat aaaaacggaa acgcaaatcc
     4741 cagaccaata gtgagaaaga aaattaacat caaaaccgat ggtaaagcta atgcaaaagg
     4801 taatgccgcc cctaaaaaag gtccggtaca agaaatagcc atcaaaaagg caaaaatacc
     4861 tccaaaaaat gaaccactgt aaccaccttt ggaggttgct ttatttgctg tattcatacc
     4921 cggaacagtt atctcaaaaa cacctaaaag cgacaaagca aatacaaaca caattgccat
     4981 cagcgttacc acaaaaccgg gattctgatt ttgtaaaccc cagcctaccg attcacccgc
     5041 tttttgtaaa gcgataaaaa ttactgccat aatagtaaat gagattaaca ccccaaaagt
     5101 gtaaaccaaa atatgatgca aaaccttggt tctatccttc tgtgcctgat tcattatgct
     5161 cattatcctt atgggtaaaa tgggtaacac gcaaggagtg atattcaata taattccacc
     5221 taaaaaggca aataggatat attttaagat ttcgccaaga gaccttttgg caataggttc
     5281 tgtcgctgcc ctatcaactt ccgaaacggg ggaagtttct cccttttcaa tattttctgt
     5341 ggcttctgtt tcttcagaaa caactccctc agcggcttca gcagtaatta tctgaaaaga
     5401 aagcttcttt gttgcttctt cggggggatc acacatacca ctttcataac aaaggttata
     5461 gcttaacaaa acttcaacct gtttgttgcc agcttttgct gtctgcttca ctgtaaaagg
     5521 taaagttaat gtaaccttgg gatgataatt ccattcttca tctgaaatga tattagtcgg
     5581 tttggggtat tcaaccttac caaaagatag gtccggatga ctggcttcta aatagaaata
     5641 ttccggattt ttaggattta cagtctgctt tttgccctca ggaattgtta aagtggcttt
     5701 aataacgcct ttttcgcctg gctttaaaac atcaggagaa agtaagaatt tgacagtttg
     5761 agcaaaaaag ggttgaatta acataatcag gcaaagtgct aacaatagct tataaccacg
     5821 attcattatc ataatccttt taacaatgat tttttattat aagttaatgc agaatttgtt
     5881 ccagacaaat tgttttatta taggatgaga aattatggaa tgtctcacgc agattacaca
     5941 gatttcgcag atggaatagg tatgggatga acaggatttt aaggatttag aggattgagt
     6001 tttagaaaca aggattttag aagaatattt agccaccgaa gccaccgaaa atagtttacg
     6061 cagaatagat tttcgaaaca aggattttag gatttta
//
LOCUS       DAORHB010000074         6057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_13141_length_6057_cov_5.069310, whole genome shotgun sequence.
ACCESSION   DAORHB010000074 DAORHB010000000
VERSION     DAORHB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13141_length_6057_cov_5.069310"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..3181
                     /locus_tag="PKM80_03280"
     CDS             254..3181
                     /locus_tag="PKM80_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92960.1"
                     /translation="MKLFRFRLIFLLLFLFIVFSTVIIAQKQTEEERYVVEDFKVEDI
                     PADDGTGLLLSWKPLERSKRIIEYRVYRGVSPDRLFFLEAIPVNVKTGVASDRMYYYD
                     NSGSEFFDITSPSELKKEKQQGKNSPLYQKMPRNIPILAEIAGKYSLLSVSEKNNYYY
                     RSQKVTLDKMPQSKTKSSFPEEGKTYAGLKDNQQTVLCFLNPGEKYYYSVVAVNERRL
                     YQKPAKITYGIPVPNPPNPAAAFHTVVLEDLKELRFEWELPIFSSDIVQYRIHKISAT
                     ADSLWNNIRYHPNVVDQHTVVLNQGLVGYGSMKNYCSVNIPQGSSLDEYKNAAYSLEL
                     IDADGYSSFSNLSKPRIKNSGNLPAKPIFHMEDKPNDKGDRLTVVWDHPICYVVKTTA
                     INDKYDKFRVNYQLNQTETQKVDNIFFEFYKNGEDKPFARINEFYQDNIVILKVPNGY
                     DYKKGFRVKIRMKGEPEIPEDYCLEQNLEYDPQMLAIMPTNALYRNGKNVSKINNIVY
                     RKSVTSPQLTLVKRNTSYDNSLDVNIPYASIIQKPVEGFSFAQGDSLITFINGERTAR
                     KLKSGDIKTPLALLSPKVDLVYDKDNATRIETNIFPDLGKKEAEEKVKELNTTLSELK
                     AQKAMLTDPAVVAQINQNIEQVQKQINAYSKNESLVSANKITDRKKRMRFIAKIREDY
                     TRWTSYQVVRSDGRGLFTESDMLKDGDDILYMKPISNVFDTNKYTTLVAMLIFGIMVV
                     VFISLAKRGKDLYIRPIAGLEEIDTAVGRATEMGRPIMYMMGHGSVSDVATIASMGIL
                     SLVARKTAEYDIKLIVPVYNYIVMPIAQEIVRDAHYSAGRPDSFDKNNVFFLTDVQFA
                     YVAGVNGIMIRERAATNFFMGYFSAEALLMTETGNSLGSYQIAGTDAVTQIPFFITTC
                     DYTLIGEELYAASSYLNHEPMQLGTLKAQDYYKFLIFAFVVVGAVLSSFQLTGLAEIF
                     PVK"
     gene            3194..3823
                     /locus_tag="PKM80_03285"
     CDS             3194..3823
                     /locus_tag="PKM80_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92961.1"
                     /translation="MKKTIPLIIVILGGIIFVIYAFSPHKLIQDDFYNKFYIPWVYAS
                     IPWAALLGVMSLTMMHSNKIKRRAPKWQYSYFFFAGFIITSLAGFIGGTQKGSALMWI
                     FENMQMPMSSTMFSLLAFYMASAAYKAFRARSAEATVLLIAAIVVMLAQVPLGVKIYK
                     NLPVISQWILDVPNLASKRGIAIGVGLGGVATSLKILLGIERSYLGGTD"
     gene            3843..5024
                     /locus_tag="PKM80_03290"
     CDS             3843..5024
                     /locus_tag="PKM80_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92962.1"
                     /translation="MNFFERMQNLDRRWIYIVVALAIIIPLMIPYNSDNVTSPPTENL
                     YQMIDSFAGREDRAVLLSFLHDASTMSELYPMEIAIIRHCFERNIKVFALSFLTSGAP
                     IIDYAFNSVKEEYPDIKSGVDYCNFGYKPQPMAIVLGMGDNIANAVNTDAEGRKLESL
                     PIMKGINNYNEMNLVVEFSGSTAGTYWIYYARPKFGVNVALGVTAVMAADEYPYLQSG
                     QLIGMLSGLKGAAEYEKLVDVFAAYRDPKVDYRVKMDAEGNKIIPGRPFGKEVLNDEG
                     TKKLINITTQTKAEFTPADYAAFVAKYPEEKAIFDSLKELEGEKIIIDVSKITPELRN
                     QMGEYAYREINQLTHNINYKFKVARIGMNAQSVAHIMIIVFIIMGNIGYFIQKSKVVE
                     K"
     gene            5042..5644
                     /locus_tag="PKM80_03295"
     CDS             5042..5644
                     /locus_tag="PKM80_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92963.1"
                     /translation="MSFELFSNLVAAFFTLCIFSFLYKDNPLYQMAEQLLVGISLGYS
                     LVFTYERIFIPYIWQPIVLKHNFILIFPTLLGILYLFRFSRKLGWLSRYPIAFSMIGI
                     GLGVPLGIHAGILVQMRQAMVPVENINLFLILLGTIAVLLYFFFSKAHTGAYGKFVGI
                     GKWYMMIGFGASFGMTVMARISLLIGRIQFLVIDVLGLMK"
     gene            5738..>6057
                     /locus_tag="PKM80_03300"
     CDS             5738..>6057
                     /locus_tag="PKM80_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92964.1"
                     /translation="MKKYLILSCIILVIAISLLSTDEAKPESLKGKVTEFSATDMPFD
                     DGAGIVLKWKPLDKSHRVIKYNIYRGVSPDSLFLLNSIEVDPKLGVLANELFYYDRGE
                     QPLV"
BASE COUNT         1864 a         1011 c         1283 g         1899 t
ORIGIN      
        1 aaaaagaatg aaatgaggtg gcagatcaca tggaaagccg attttaactc aaaggccgac
       61 acaatgatag cgatggtggc gtaagcccct cgaaaattag cacaaaatga aataaagttc
      121 cgctaggaac gatacatact atccaagggt tttaacacag aggaaaaaag agattgacat
      181 aattgggcaa tttctgtgtt tggaacgcag tagaaaataa gctgtttata atgatatttg
      241 ggagaaatag catatgaagt tattcagatt tcgcctaatc tttctccttc tgtttctatt
      301 catagtgttt agcacagtga taatagccca gaaacagacg gaggaggaaa gatatgttgt
      361 agaagatttc aaagtagaag atattcctgc tgacgatgga acgggattat tattatcctg
      421 gaagcctctt gaacgaagca aacgcattat cgaatatcgt gtttaccggg gagttagtcc
      481 tgacagatta tttttcctgg aagccatacc tgttaatgta aaaacgggtg ttgcatcgga
      541 taggatgtat tattatgaca attctggtag tgaatttttc gatattacct ctcccagcga
      601 gttaaagaag gaaaaacagc agggtaagaa tagtcctcta tatcaaaaaa tgcctcgtaa
      661 cattccaatt ttagcagaaa tagcaggtaa atattctctg ctttccgtta gtgaaaagaa
      721 caattactat tacagaagcc agaaagtaac tctggataaa atgccacaat caaaaacgaa
      781 gagttctttt cctgaagagg gaaagacata tgctggtttg aaggataatc agcaaacggt
      841 tctttgtttt ctaaatcctg gcgaaaagta ttattattca gttgtagcag taaatgagag
      901 gcgtttgtat caaaaacctg ccaagatcac ttatggaatt ccggttccca atcctcctaa
      961 tccagctgct gcttttcaca ctgtagtttt ggaagaccta aaagagttaa ggtttgaatg
     1021 ggagcttcct atattcagtt cggacatagt tcaatatcga attcataaaa tatcagctac
     1081 tgcagattct ttatggaata atatacgcta tcatcctaat gttgtagatc agcatacggt
     1141 tgtgctcaat cagggtttag taggttatgg ttcaatgaag aattactgca gtgttaacat
     1201 tccccaaggt agttccttag atgaatataa aaatgctgct tattcactgg aattaataga
     1261 tgcagatggc tacagcagtt tttccaatct ttccaagccg cggatcaaga atagtggtaa
     1321 tcttcctgcc aagcctattt ttcatatgga agataaacct aacgataaag gtgatcggtt
     1381 aacagttgtt tgggatcatc ctatttgtta tgtagttaaa accacggcaa taaacgataa
     1441 atatgataaa ttcagagtga attaccaatt gaatcagacc gaaacccaaa aagtggataa
     1501 tatctttttt gagttttata agaatggcga agataagccc tttgctcgaa tcaatgaatt
     1561 ttatcaagac aatattgtga ttttaaaggt ccccaatggt tatgattaca aaaaggggtt
     1621 ccgagtgaaa attagaatga aaggcgagcc cgaaattcca gaggattact gtctggagca
     1681 aaatttggaa tatgatcctc aaatgcttgc cattatgcct actaatgcac tataccgcaa
     1741 tgggaaaaat gtttccaaaa ttaacaatat cgtttatcgt aaaagtgtta cttctcccca
     1801 gttaactttg gttaaacgca atacatcata tgataactct ttggatgtaa acattcctta
     1861 tgccagtata attcaaaagc cggttgaggg attcagtttt gcccaaggtg attcgctgat
     1921 tacttttatt aatggtgaac gaactgctcg caaactgaaa agtggagata taaaaactcc
     1981 cctcgcttta ctttcgccca aggttgatct agtttatgat aaggataatg caacccggat
     2041 cgaaacaaat atatttcccg atttaggtaa aaaggaagcg gaagaaaaag taaaggaact
     2101 gaatacaacg ctgtcggagt tgaaagctca aaaagcaatg ttaactgatc ctgcagtagt
     2161 tgcccaaata aatcaaaaca tagagcaggt tcagaaacaa ataaatgctt acagtaaaaa
     2221 cgagagcttg gtttccgcta ataaaattac cgaccgaaaa aagcggatgc gctttattgc
     2281 taaaattaga gaagattata cgcgctggac aagctatcag gtagttcgtt ctgatggtag
     2341 aggtcttttt actgaaagtg atatgttaaa agacggcgat gatattctat atatgaaacc
     2401 gatttccaat gtgtttgaca caaataaata tactaccctc gtagcaatgt tgatatttgg
     2461 gataatggtg gtggtcttta tcagtttagc caaacgcggc aaggacctat acattcgtcc
     2521 catagcaggt ttagaagaaa ttgatacagc tgttggcaga gccacagaaa tgggtcgccc
     2581 gataatgtat atgatgggtc atggtagtgt tagtgatgta gcaacaattg cttcaatggg
     2641 tattttaagt ttagttgctc gcaaaacagc tgaatatgat ataaaactta tcgtgcctgt
     2701 ttacaattat atagtgatgc ctattgccca agaaattgtt agagatgcgc attattctgc
     2761 aggccgtcca gatagctttg ataaaaataa tgtattcttt ttaaccgatg ttcagtttgc
     2821 ttatgtagca ggtgtgaatg gaattatgat tcgtgagcga gctgccacga atttctttat
     2881 gggttatttt agtgcggaag cattgttgat gacggaaaca ggaaattccc tgggttctta
     2941 tcagattgca ggaaccgatg ctgtaaccca gattcctttc tttataacta cttgtgatta
     3001 tactttaatc ggagaagagc tttatgctgc ttcttcttat ttaaatcatg aaccaatgca
     3061 actgggaact ttgaaggcac aggattatta taagtttctc attttcgctt ttgtggttgt
     3121 cggagcggtg ctttccagtt ttcagttaac aggtcttgca gaaatatttc ccgttaaata
     3181 aatgaggtct tagatgaaga aaacaatacc tttaatcatt gtaatcttgg gtggcataat
     3241 ctttgtgata tatgccttta gtccccacaa gcttatacag gatgattttt acaacaagtt
     3301 ttacattccc tgggtttatg cttccatccc ttgggctgca ttactgggag taatgagttt
     3361 aactatgatg cacagcaata agataaaaag gcgtgctcct aaatggcaat atagctattt
     3421 cttttttgct ggattcatta ttacttctct tgctggtttt attggtggaa cgcaaaaagg
     3481 aagtgctttg atgtggattt ttgagaatat gcaaatgccg atgagctcta cgatgttttc
     3541 gttattggca ttttatatgg catctgcagc ttataaagca tttagggctc gttctgcgga
     3601 agcgacggtt ttactgattg ctgctattgt agtgatgttg gcacaagtgc ctttgggagt
     3661 gaaaatctat aaaaatctac cggttatttc tcaatggatt ttggatgttc ccaatcttgc
     3721 ttccaagcgt ggaattgcta ttggagtagg tcttgggggt gtggcaactt ccctaaaaat
     3781 acttttgggc atagaacgtt cctacttggg tggaacggat taaggaaagg ggggaaaaca
     3841 ggatgaattt ttttgaaaga atgcaaaatt tagaccggcg ctggatctac attgttgtag
     3901 ctctggcaat catcattccc ttaatgattc cttataattc ggataatgta acttctccgc
     3961 caacggagaa tctgtatcag atgatagatt cttttgccgg cagagaagat agagccgtgt
     4021 tattaagttt tttacatgat gcttccacga tgtctgagct ttatccaatg gaaattgcca
     4081 taatcagaca ttgttttgaa cgcaatatca aggtcttcgc tctctctttt ctaacttcgg
     4141 gagcacctat tattgactat gctttcaatt ctgtgaaaga agaatatcca gatattaaaa
     4201 gtggagtgga ttattgtaat tttggctata agcctcagcc tatggccatt gttttgggta
     4261 tgggagataa tattgccaat gctgtaaata cggatgctga aggacgcaag ctggagagtt
     4321 tgccgattat gaaaggaatt aataactaca acgaaatgaa cctggtagta gaattttccg
     4381 gaagcacggc aggaacttat tggatatatt atgctcgtcc caaatttgga gtgaatgtag
     4441 ctttgggagt taccgctgtt atggctgctg atgaatatcc ttatttacaa tcgggtcaat
     4501 tgattgggat gctttctgga ctaaaaggtg cagctgaata tgagaagctg gttgatgttt
     4561 ttgccgctta tcgtgatccg aaagttgatt atagagtaaa gatggatgcg gaaggcaaca
     4621 aaattattcc ggggcgtcct ttcggcaaag aagtgctcaa tgatgaaggc acgaaaaaac
     4681 taattaatat taccactcag accaaggcgg aatttactcc tgccgattat gctgctttcg
     4741 tagccaaata tccagaggaa aaagctattt tcgattctct caaagagctc gaaggggaga
     4801 aaataattat tgatgtaagt aaaataaccc ccgagctaag aaatcagatg ggcgaatatg
     4861 cctatcgcga aataaatcag cttactcaca atataaacta taaatttaaa gttgccagaa
     4921 ttggaatgaa tgctcaatct gttgctcaca taatgattat agtttttatc attatgggta
     4981 atattggtta tttcattcag aaatccaaag tggtagaaaa gtaaagaacg ggaggataac
     5041 aatgagtttc gaattattta gtaatctagt ggctgcgttt ttcacgctat gtattttctc
     5101 cttcctgtat aaggataacc cactctatca gatggcggaa caacttttgg taggaatttc
     5161 cttaggttat tctttggtct ttacttatga acggatattt attccttata tatggcaacc
     5221 gattgtgctc aagcataatt ttatccttat ttttcctacc cttttaggaa tactgtatct
     5281 gttccgtttt tctcgtaaac tgggttggct ttctcgttac cctattgctt tttcgatgat
     5341 tggaattgga ttgggtgttc ccttaggaat tcatgctgga attttagttc agatgcgtca
     5401 ggcaatggtt cctgtagaga acatcaatct ctttcttatt ttattaggca caattgcagt
     5461 gttgttatac ttcttctttt ccaaagcgca tactggtgct tatggaaagt ttgtaggcat
     5521 tgggaaatgg tatatgatga ttggttttgg agcttcattt ggaatgacag ttatggctcg
     5581 tatctcattg ctgattggta gaatccagtt cctcgtaatt gatgtgctgg gactaatgaa
     5641 atagctgtta tttccaggaa ccggtaaaaa aggtaagaca aaaaaacaaa ggttcttacc
     5701 caattactta ctggaatcct ggctaaggga tagataaatg aaaaaatacc tgattttaag
     5761 ctgtataatc ttagtgatag caatttcatt gctttctaca gatgaagcca aacccgaatc
     5821 cctgaagggc aaagtaactg aatttagtgc caccgatatg ccttttgacg atggtgctgg
     5881 aatcgtttta aaatggaaac ctttggataa atcacaccgt gtgattaaat ataatatata
     5941 cagaggcgtt agccctgatt ctctgttttt acttaacagc atcgaagttg atcctaaact
     6001 gggtgtttta gccaatgaac tattctatta tgaccgtggt gaacaaccat tagtaga
//
LOCUS       DAORHB010000075         6032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_13234_length_6032_cov_5.087502, whole genome shotgun sequence.
ACCESSION   DAORHB010000075 DAORHB010000000
VERSION     DAORHB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13234_length_6032_cov_5.087502"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /gene="pyrR"
                     /locus_tag="PKM80_03305"
     CDS             <1..542
                     /gene="pyrR"
                     /locus_tag="PKM80_03305"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNV92965.1"
                     /translation="KMQVKSQIMDKDQMERSLQRMAHEIIEQNRGLEKIRLVGIRSRG
                     VPLAERLSSYLKLITNQEIPVGILDITLYRDDLSTISHQPIIKGSAIDFDIEDSIIVL
                     VDDVLYTGRTVRAAIDALMDFGRPKQIQLAVLIDRGHRELPIKADYVGKNVPTSKEEI
                     IKVALEEIDGEDSVQIVLV"
     gene            508..1308
                     /gene="pyrF"
                     /locus_tag="PKM80_03310"
     CDS             508..1308
                     /gene="pyrF"
                     /locus_tag="PKM80_03310"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNV92966.1"
                     /translation="MGKIVSKSFWYKYQERYNNTKSVLCVGLDSDFNQLPKLLKNAQN
                     PIWEFNKAIIEATLDYACAYKPNLAFYLADGLRGLDALYKTVDFIPDNIPVILDVKVG
                     DIGNTMNAYLTAFFKDLEVDAITLNPLMGKDVMQPVLEKNGFAFVLCLTSNPGASDFL
                     KDGMAEKIANWLQNYPKENFGAVVGATQSADLKKMRNLLQDRILLIPGIGAQGGDLKT
                     VLQQATYSEATPNILVNSSRGIIFASQDHNFASAAREEARKIAESEYL"
     gene            1493..3286
                     /locus_tag="PKM80_03315"
     CDS             1493..3286
                     /locus_tag="PKM80_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNV92967.1"
                     /translation="MPGINCLINAKGITPQNRNTIDLHLQRFGATFPHLQLANFMPCS
                     EVLLTFSQHQGYQVYTESYKGWQIFHELQDDVLITQTVTDILEQVENNSISLANLRKS
                     WNMLLHNTYESFFLLAVNPDLHKIIFANDALARLPIYYTQQKDCFILGRDISWVKAIS
                     DQLTLNPLFMALYLSNAYIPGRGTFYQEIDTLASGTFAIYDWLTDDLQMISQPNMRFV
                     EPLAGRAEKKRLNELVETFMDVCVSYRTKLPKLLSLSGGMDSRCVAGALQQKSIDFVP
                     ISFLDFQKEVKDDVLIASQIAELYHKSHNVIQLSLCEPEHYEKLFYLKAGLNYLPVAM
                     FLQYLEKILAQYPQSALFLTGDGGDKVMRYLLPDKELADEKQWLNYWYSQNAIIPTKD
                     SAAIFGIPEKAMDEYLLNHLSTYPTGNYNYKYASAILAERSARWAFEGEDRNRYFFRS
                     ETPFFDFRFYQLAMQIPMEQKKDNVLYYNFLYALSPSLAKLRYARYQWSPAKMRNIFY
                     RILVNQTRKIRLKYRSHKEAINHQQSFAEQDYIINWILEQSQKSIVKEIMPGAKSFLN
                     LEYLQNLSPSQLGTLFTSISVITGGPISPQK"
     gene            3389..4630
                     /locus_tag="PKM80_03320"
     CDS             3389..4630
                     /locus_tag="PKM80_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV92968.1"
                     /translation="MRKIFILMLFSWAVICSAFQVDVFGPEADDFNCANLDQEPASFG
                     IRIDDQDKLCLWENGEWNSYSYYVNGLPVRSTCMLNADTMLVAMGAMTYSDGIYNFDI
                     HTHTWQLNDWFLLPNFVKYNPNSQLYYVGERDGLYRSTNGHNWSRINSLGLNECSSLA
                     WYGNNIITNNGQIVYYTNDNGLNWHQSSISNLHSFRFDDNGILYAIMDVTSDSDGLWR
                     SNDFGANWVNILYTSALVCIGPVFNNNVTLGWRECYEDSSYVGMFRSNGTVSHFNHPS
                     LNKPVKQMDIFPYIDTFSFYVLNSDGCFYLTSFLVGIEDPVIPPIPIAKISAYPNPAT
                     AYANIAIYKNSPEPAELNIYNLKGQLVRSIEISGVTKESTINWDLHNLNSTKVAAGIY
                     LLVLQDKAGKPLASNKLAVVK"
     gene            complement(4635..5360)
                     /locus_tag="PKM80_03325"
     CDS             complement(4635..5360)
                     /locus_tag="PKM80_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNV92969.1"
                     /translation="MRFDVSYFCHYGNRALKNQDRVIVNGDIVSCGVKHQENIAALFC
                     FVADGIGSLINSENAAQYVLEQIDFLRLRSGLDWCNLIDSFLQNTNLELVKMNHERDD
                     FEDSATTLCGLIYRDKIFIPINVGDSEIWLYRNKELLRITKPHIEDECIPNSPINNYF
                     GSFQAHMYIDFITSIPCLLPDDKLVVTTDGLLKAISKEELIHILEDNAPLKNKINALF
                     YQLSAKDNPDNLGAIFIQVKKDK"
BASE COUNT         1890 a         1047 c         1145 g         1950 t
ORIGIN      
        1 gtaaaatgca agtaaaaagt caaataatgg acaaagacca aatggagcgt tccctacaaa
       61 gaatggctca cgaaattatt gaacagaacc gcggactgga aaaaattcgc ttggtgggta
      121 ttcgttccag aggagttcct ttggctgaaa gactttccag ttacttaaaa cttattacta
      181 atcaggaaat accggttggt attttggata ttactcttta ccgtgatgat ctttcaacaa
      241 ttagtcatca acccataata aaaggttctg ccatcgattt tgatattgag gattcaataa
      301 ttgtgttggt ggatgatgta ctttatacag gaagaactgt tcgagctgcc attgatgctt
      361 tgatggattt tggcagaccg aaacaaattc aattggctgt attaattgat cgcggtcatc
      421 gcgaattgcc tattaaagcg gattatgtag gtaaaaatgt ccctacctcc aaagaagaaa
      481 taattaaagt agctttagaa gaaatcgatg gggaagatag tgtccaaatc gttttggtat
      541 aaatatcagg aacgctataa taatacaaaa tcggttctct gtgtaggcct ggattccgat
      601 tttaaccagc ttcctaaatt gctgaaaaat gcccaaaacc caatttggga attcaataaa
      661 gccataattg aagccacttt ggattatgct tgtgcttata aacccaattt agctttctat
      721 ctggcagacg gattacgcgg tttggatgct ttatacaaaa cagtagattt tattccggat
      781 aatattccag ttatattgga tgtaaaagtt ggtgatattg gcaacacaat gaatgcctat
      841 ctaacggcat tttttaagga cctcgaagtt gatgctataa ctcttaatcc acttatggga
      901 aaagatgtaa tgcaaccagt tctagagaag aacggttttg cctttgtttt atgcttaact
      961 tccaatccgg gagcatccga ttttttgaaa gatggtatgg cagaaaaaat agctaactgg
     1021 ctgcaaaatt atcccaaaga aaattttgga gctgtggtgg gagcaacgca aagtgcagac
     1081 ttgaaaaaga tgcgtaattt attgcaggat cggatattac ttattcctgg aattggagct
     1141 caagggggag acctaaaaac tgttttacag caggcaacat atagtgaagc aacacctaat
     1201 atcttagtta atagctcgcg gggaatcatt tttgcctcac aggatcataa ttttgcctct
     1261 gctgccagag aggaagcaag aaaaatagcg gaaagcgaat atttataagt atcacagaga
     1321 gaatgaatct ccctgttcct tggttaaaat aattgcttaa gggatttcct ctctctatct
     1381 taagatattc gagtagatat tgatttcaac agcattatcc caattggatt ccgaatcgtt
     1441 ttttaaaacg gataaaacca aagttgaatc ttctggaaat taaggagaac caatgccagg
     1501 aataaattgc ctgattaatg ccaaggggat tactccacag aaccgaaata ctatagatct
     1561 ccatctgcaa aggtttggag ctacttttcc tcatttgcaa ttagcgaatt ttatgccttg
     1621 tagtgaagtg ttgttaactt tttctcagca tcagggctat caggtttata cagagagtta
     1681 taagggctgg caaatatttc acgaactaca ggacgatgtt cttataacgc aaactgttac
     1741 cgatatttta gagcaggtag aaaataattc tatatcttta gcaaatctaa gaaagagctg
     1801 gaatatgctt ctccacaata cttacgaaag tttctttttg cttgccgtga atccagattt
     1861 gcataaaata atctttgcca atgatgcttt ggcgcgtctt cccatttatt atacccaaca
     1921 aaaggattgt tttattttgg gcagggatat ttcctgggta aaagcaatca gcgatcagtt
     1981 aaccttaaat cctttattta tggctttata tctttcaaat gcctatattc ccggacgcgg
     2041 aaccttttat caggaaatag atactctggc aagtggaact tttgccattt atgattggtt
     2101 aactgacgat ttacaaatga tttcccaacc aaatatgaga tttgttgagc cccttgccgg
     2161 tagagcagaa aaaaaaaggt taaatgaact tgtagaaaca tttatggatg tatgtgtttc
     2221 gtatcggaca aaacttccca aacttttgtc tttaagcgga gggatggatt cgcgttgtgt
     2281 ggctggagct cttcaacaaa aaagtataga ttttgtccct atatcttttc tggattttca
     2341 gaaagaggta aaagatgatg ttctgattgc ttctcagata gccgagttat accataaatc
     2401 tcataatgtt atacagcttt ctctttgtga accggagcat tatgaaaagt tgttttattt
     2461 aaaggcaggt ttgaactatc ttccagtagc tatgtttctg caatatttgg agaagatttt
     2521 ggctcaatat cctcaatctg ctttgttttt aactggagac ggtggcgaca aagttatgcg
     2581 ttatctttta cctgataaag agttagccga tgaaaaacag tggctgaatt attggtatag
     2641 tcagaatgcc atcatcccca ccaaagattc agcagcaatt tttggtattc cagagaaagc
     2701 gatggatgaa tatcttttga accatctatc tacttatccg acaggaaatt ataactataa
     2761 atatgcttct gccattttag cggagcgttc tgctcgttgg gcttttgaag gtgaagatcg
     2821 taatcgttat ttcttccgtt cagaaacacc ttttttcgat tttaggtttt accaattagc
     2881 aatgcagata ccaatggagc agaagaaaga taatgtgcta tattacaatt ttctatacgc
     2941 attatcgcct tccctggcaa aattaagata tgcgcgttat caatggtctc ctgccaaaat
     3001 gcgaaatatt ttttaccgca tattggttaa ccaaactcgt aaaattcgct taaaatatag
     3061 aagccataaa gaagcaatca atcatcagca aagttttgct gaacaggatt atataataaa
     3121 ttggattttg gagcagagcc aaaaatccat tgtaaaggaa attatgcccg gtgcaaaatc
     3181 atttctcaat ttagagtact tgcaaaatct atctcctagt caattgggta cattgttcac
     3241 cagcatttct gtgattactg gaggcccgat atcaccacaa aagtaaatta atcttgtgca
     3301 agtggttaat ttgctttttc tttcttttca tttacattat atatagaatt gatattctga
     3361 actcattttt ctggaagagg aggaagatat gaggaagatt tttattctta tgctatttag
     3421 ttgggcggtt atatgttctg ctttccaggt tgatgtattt ggtcctgagg cagatgactt
     3481 taattgtgcc aatttagacc aggagcccgc cagttttgga attaggatag atgatcaaga
     3541 caaactttgt ctttgggaaa atggtgaatg gaatagctat tcctattatg ttaatggttt
     3601 acctgttaga agtacttgta tgctgaatgc agacacaatg ctggttgcta tgggggcaat
     3661 gacatacagt gatggaattt ataactttga tatacacacg catacctggc aattgaatga
     3721 ttggtttcta cttcctaatt tcgtaaagta taatcccaat agtcaattgt attatgtggg
     3781 cgagcgtgat ggtttgtatc gttcaacgaa tggacataac tggtcacgga ttaattctct
     3841 cggtttaaat gagtgtagtt cccttgcctg gtatggtaac aatattataa cgaataatgg
     3901 ccaaatagtt tactatacta atgataatgg acttaactgg catcaatcat caattagcaa
     3961 tctgcatagt ttcaggtttg acgacaatgg cattttgtat gctattatgg atgtaacaag
     4021 tgattcggat ggactttggc gttccaatga ttttggcgca aactgggtaa atattcttta
     4081 tacttccgcc cttgtttgca tagggcctgt tttcaataac aatgtaactc ttggctggag
     4141 agaatgttac gaggatagtt cctatgtggg aatgtttcgc tccaatggaa ccgtatctca
     4201 ctttaatcat ccttctctca ataaaccagt taaacagatg gatatttttc cctatatcga
     4261 caccttttcc ttttatgtgt taaattccga tgggtgcttt tatttaacca gttttttggt
     4321 aggaatagaa gatcccgtaa ttccgccaat acccatagca aaaatatctg cttatcctaa
     4381 tccagcaacg gcttatgcta atattgccat ttataaaaat agccctgaac ctgccgaact
     4441 gaatatttac aacctgaaag gtcaattggt gagaagtata gaaatatcgg gagttacaaa
     4501 agagagcacg ataaactggg atttacataa tcttaattcc acaaaagtgg cagcggggat
     4561 ttatctattg gttttacagg acaaagccgg aaaacctttg gcaagcaata aactagcagt
     4621 agttaaataa tgggttattt gtcctttttt acctgaatga agattgctcc cagattatca
     4681 ggattatctt tagctgatag ctgataaaaa agagcattta ttttgttctt cagcggtgcg
     4741 ttatcttcca atatatgaat aagttcttcc ttgctaatag ctttcaaaag accgtctgta
     4801 gtaaccacca gtttatcatc cggtaataaa caaggaatag aagtaataaa atcaatatac
     4861 atatgagctt ggaaggaacc gaaataattg tttatagggc tattaggaat gcattcgtct
     4921 tctatatgag gttttgtaat gcgcagtagt tctttgttcc gatatagcca aatttccgaa
     4981 tcacccacat ttatgggaat aaaaatcttg tccctgtaaa taagcccgca aagagttgta
     5041 gctgaatctt caaagtcatc cctctcgtga ttcattttca ccagttccag attagtattt
     5101 tgtaaaaagg agtcaattaa attacaccaa tcaagaccag acctcaaacg cagaaaatca
     5161 atctgctcca aaacatattg agcagcattc tcactattaa ttaaagaacc aattccatct
     5221 gctacaaaac aaaatagagc agcaatgttt tcctgatgct ttactccaca ggatacaata
     5281 tccccattca caataactct atcctgattc tttaatgctc tattgccgta atgacagaaa
     5341 taggatacat caaatctcat ataaattctc ctaagcacaa ttatagacaa atgtaagcgc
     5401 gatcaccaat gaaggtaaca attatttgca ctattttctt ggcaagtact tttttccaca
     5461 gttgaaattt ctttaatttt catcccagac ggtaagttta taataatata gagtgcttta
     5521 taattaacat tatatcctat agctatgtta tgattcttaa cctgcactgc agtagaaact
     5581 tatcctatgc aaacttctga atactagctg taatcataat tttgtaagtg atccagtttt
     5641 ctaccaattt gtaactgtat tagctgtgtt aagtgcatta gtgaaattca gtttagttgc
     5701 taagagagga ttggttagcc catttttcct aaagtgtaaa actttgggtc gctattatgt
     5761 tatatcctaa tatatttatc taccctaagg tctttgctac tataatagac agcatatcgg
     5821 aagccagctt cgcttttctt tcttcattcc aagcgtcaat caagtgttaa tctaacctta
     5881 atcaagcgtt aattgttaac gcttgattaa gggatgatga acgctatatt aacatcctca
     5941 agcaaggaag atagaagaca gaaatatgga gattttaggt ctattgaaag aaatgtagtt
     6001 gctatttagc gagtaaatag tcgatgattt tc
//
LOCUS       DAORHB010000076         6016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_13301_length_6016_cov_4.897668, whole genome shotgun sequence.
ACCESSION   DAORHB010000076 DAORHB010000000
VERSION     DAORHB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13301_length_6016_cov_4.897668"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PKM80_03330"
     CDS             complement(<1..1100)
                     /locus_tag="PKM80_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92970.1"
                     /translation="MKRTVLMALMVGFVMASSLQGENLSASLHTNIETENIAYEDYSP
                     LTEKDIYELKNLLQEIQLNPSDLNFEKDWDLSTWGKSEEFLHALQEPYYGLKKMQEYR
                     NAANKSDTDDGFAFLADLLYKNAIDDSGSISIYREYKQIYQQNFWREVQQPQDLFPFY
                     ETALKDIIPILAEAYANYNSELWSKYISYLFTALIESEDKEAYSTYFNQHNIPETRDV
                     EDCEIKKQMGLLLEPAILKAGMQFLALGDVMKNEAGKLLYKNKKPIIKTGKFGTMIIG
                     TVHDDVYSEKLLKKPLILLIDPSGNDKYEFSLATGYDNYTYLLIDYKGDDSYLNPKIG
                     EMFFALSGLGISYDLQGNDIYKTGDFSFAAMMG"
     gene            1574..3673
                     /gene="recG"
                     /locus_tag="PKM80_03335"
     CDS             1574..3673
                     /gene="recG"
                     /locus_tag="PKM80_03335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNV92971.1"
                     /translation="MAESKKTTDLHSEIKYLKGVGENRARLLGKLGIKTILDLMEHFP
                     KLYISRKLSAHLEDLKPGDMLAFTAMISWVDVHTSLKGKKILNIGVSDGKVGLICSWF
                     TYPPVYEKLFIPGRLVWLNGTISEFNGQLQMVHPDFELIDDSEDTKDDFWKKREVLPV
                     YPLTEGISQKLMRQLMYNAFSLYTGLIEENLPEYILTKHHFLERHIALQKMHFGQNPQ
                     EIEIVRRRFAYEDFFYSQLLWARHKLFHTTTTTGINFINKKQLTTALYRKLPFTLTSA
                     QKKVLWEIFNDMCSEKQMSRLLQGDVGSGKTVVSLFAMLLAVENDYQAAMMAPTEILA
                     EQHYETISQLLKDSEIKVCLLKGGVYKGKEETKNAIAEGEAQIVIGTHALIQKDISFK
                     RLGFACVDEQHRFGVEQRARLARQALHPDLLYLSATPIPRSLAMTVYGDLEVSILDEL
                     PPTRKPVRTIIRPSSKIDTVYIEVRKELEKGRQVYIVCPLVEESEKISLLDATKLYEY
                     ISKKIFPEYPACLLYGKMPVKEKDAIMKRFKAGEIKILVSTTVIEVGVDVPNANVMII
                     EHSEHFGLAQLHQLRGRVGRGSEQAYCYLIEHQPVNDVAWQRLTTMTKTTDGFIIAEK
                     DLELRGPGDLFGFEQSGMPQFRFANIVRDQEILKIARQDAFEVVRSDPDFSQIKNAIV
                     KKMYHHQYTKKEELILY"
     gene            3752..5989
                     /locus_tag="PKM80_03340"
     CDS             3752..5989
                     /locus_tag="PKM80_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNV92972.1"
                     /translation="MKKLSILLMLIIAIGVLTAINFDPELFYSKTIIACFTKTAIPNL
                     DGVVNFDVQDGIISTGMSSFDQLAKELHIVDMKQMHPYVKVPEWNDNGIYLQNHYRLY
                     LESDDNMDKAVELLSKDPNLVYAEFEGINRPKYVTNDPMLDLQYAHTLVHSFEAWDYT
                     LGSHDVLVAITDSGVKWNHPDLRANIWINPAESPGMTIDWNAGTISGGNGVDAGEGGN
                     KVDDLVGWDFYNNDNNPMQNFAANDHGTHVAGCAGAVGDNEIGVVGTCPNVSILCCKG
                     SSNTSPSTGVSYAYDQVKYAGEIGADIINASWGSVGSGTYPNSIVNYVTALGALVVTA
                     AGNENTEHNASYQDYPADCTNALCVAAVAQGDVKASFSDYGSTIDICAPGAAILSTII
                     TGDGYAAYDGTSMASPVAAGVAALVKSLNPNLTPVELMQRLMATADNIDSLNPDYVGL
                     LGAGRINAYTATMYDKIPNMQIEDYILEEISGDDDGVANPGEVIKLRIALSNCIDPIT
                     GLGWLQAENVTVTMRCHYPGVTIIDSVATFGTMHAGTTLLNNNQPFKFQTVSTLPSEP
                     IPFQFYVQANQDNQYPYNKALPIDISLSLNQAGWPFYSGAATNSTPLLIDMDDDGQKE
                     VVFVGADGNIEVLHKDGLTSYPGFPVQTSSVIIGSIAMGNINNDSNREFVACLQNNQV
                     ICFNDLGEVKWNNASGGTLRNGPVIFRPNAASELKVACVTQNGILNIFNGDGSNFTNF
                     PVTEN"
BASE COUNT         1896 a         1018 c         1154 g         1948 t
ORIGIN      
        1 cccatcattg cagcaaaaga aaaatcacct gttttataaa tatcatttcc ctgtagatca
       61 taggaaattc ccaagcccga taaagcaaaa aacatctccc ctatttttgg gttcaaatag
      121 ctatcatctc ctttataatc aatcagaaga taggtatagt tatcataacc ggtagccaga
      181 gaaaattcat atttatcatt tcctgaggga tcaataagca gaataagtgg cttttttaat
      241 agtttttcac tataaacatc atcgtgaacc gttccgataa tcattgttcc aaatttaccg
      301 gttttaatta tgggtttctt atttttatag agtagttttc ctgcttcatt cttcatcaca
      361 tcccctaaag ccagaaattg catacctgct ttcaagatag ctggctccaa aagaagcccc
      421 atttgtttct tgatctcgca atcttcaaca tctcttgttt caggtatatt atgctgatta
      481 aaataggtag agtaagcttc tttatcttca ctttctatca gagccgtaaa tagataggaa
      541 atatatttac tccacagctc agaattataa ttcgcataag cctcagccaa aatagggata
      601 atatccttca gagcagtttc ataaaaagga aaaaggtctt ggggctgctg aacttcacgc
      661 caaaaattct gctgatagat ttgtttatat tctcgataaa tagaaatcga gcccgaatca
      721 tcgatagcat ttttatatag taaatctgcc aaaaaagcaa agccatcatc cgtatctgac
      781 ttatttgctg catttctata ttcctgcatt tttttcaaac cataataagg ttcctgcaag
      841 gcgtgcaaaa attcttcgct cttaccccag gtagaaaggt cccaatcctt ctcaaaattg
      901 agatctgaag ggtttaattg aatttcttgc aacagatttt ttagttcata aatatctttt
      961 tcagtaagag gagaataatc ttcgtaggct atgttttctg tttcaatatt tgtatgcagt
     1021 gaagcagaca gattttctcc ttgaagactg gatgccatta caaaaccaac cattaatgcc
     1081 attagaacag ttctcttcat aatatcctca tatattggtt caatatatat tatacttgtt
     1141 ttgttgtcaa tctttagatt tggctaaatg cgtactttat cccgaaatag aaacagacag
     1201 atctacctct ttttatttgt aaaacagata acttccccat taaccaaaat actatttttt
     1261 ttagataaac actcttgttt cttccttgat agctaccctg ctgttacctt ggtgctgatg
     1321 ggcaaaaacc cgagcagccc tacggcaatt ccaccgcaga taattaagaa ggactggttt
     1381 tttatagctt atattttagc ttaatataga gtaagataca atcactttta tgtagattgc
     1441 caagctgcaa ataaggatat gacaataata aaattggtat catctcccac tttaggtata
     1501 gttcttcaca taatttaact ttgttaatgc ggagattgac aaatccagct tattatctat
     1561 attggatttt attatggcag aaagcaaaaa aactacagat ttacatagcg aaattaaata
     1621 cctgaaaggg gttggcgaaa atcgagcccg acttttaggt aagctgggta ttaagaccat
     1681 tttggactta atggaacatt ttcctaagtt atatattagc cggaaattaa gtgctcattt
     1741 agaagatttg aaaccaggtg atatgcttgc ttttacagct atgatttctt gggttgatgt
     1801 tcatacttct ttgaaaggca aaaagatact caatataggt gtcagtgatg gaaaagttgg
     1861 gcttatatgt tcctggttta cctaccctcc cgtatatgaa aaattgttta tcccgggcag
     1921 attagtttgg ttaaatggaa caatttctga atttaatggc cagctgcaga tggtgcatcc
     1981 agattttgaa ttgattgatg atagcgaaga tacgaaagat gatttttgga aaaagcgaga
     2041 agttttacct gtttatcctt taacagaagg tatttctcaa aagttaatgc gtcagcttat
     2101 gtataatgct ttctctcttt atactggttt aatagaggaa aatttgccgg aatatatatt
     2161 gacaaaacat catttcctgg aaagacatat tgcccttcaa aagatgcatt ttgggcaaaa
     2221 tccgcaagag attgaaatag tccggcgccg ttttgcctat gaggattttt tctattccca
     2281 gcttttatgg gcgagacata aactgtttca tactacaaca accacaggaa ttaattttat
     2341 caataaaaaa cagctcacaa ctgctctcta tagaaaactg ccctttacgc taactagtgc
     2401 acagaaaaaa gtgttatggg aaatatttaa tgatatgtgt tccgaaaaac agatgagtcg
     2461 tttgcttcaa ggtgatgtag gtagtggaaa aacagtggta tctctttttg caatgctttt
     2521 ggctgtagaa aatgattatc aggcagctat gatggctccc acagaaattc ttgctgaaca
     2581 acattatgaa acaatttccc aacttttgaa agattctgag attaaggtct gcttacttaa
     2641 gggtggagta tataaaggta aagaggaaac aaaaaatgca attgccgaag gtgaagctca
     2701 aattgtgatt ggaacacatg ccctgataca gaaagatata agttttaaac gcttaggatt
     2761 tgcctgtgtt gatgaacaac atcgttttgg ggttgaacaa agagcgcgtt tagctcgcca
     2821 ggcattacat cctgatttat tatatttatc tgcaactcct attccgcgca gtttagctat
     2881 gaccgtttat ggtgatttgg aagtgagtat tttagatgaa cttcccccca cgcgaaaacc
     2941 agttagaaca attatccgtc cttctagtaa aattgataca gtttatattg aagtgcgcaa
     3001 agagctggaa aaagggaggc aggtttatat tgtttgccct ttagtagaag aatcagaaaa
     3061 aatatctctt ctggacgcta ccaagcttta tgaatacatc tccaaaaaga tatttccaga
     3121 ataccctgcc tgtttattat acggaaagat gcctgtgaaa gaaaaggacg ctattatgaa
     3181 gcgttttaaa gcgggagaaa taaaaatact ggtttctact acagttattg aagttggagt
     3241 agatgttcct aatgccaatg taatgattat agaacattct gaacattttg gtttagctca
     3301 attacatcaa ttgcggggta gagtgggaag aggtagtgaa caagcatatt gttatttaat
     3361 tgaacatcaa cctgttaacg atgttgcctg gcaaaggctt accactatga ctaaaactac
     3421 cgatggtttt attatagcag aaaaagattt ggaattgcga ggtccgggtg atctttttgg
     3481 ttttgagcaa tcaggaatgc ctcaattccg ttttgctaat attgtgcgcg atcaagaaat
     3541 actaaaaata gctcgtcagg atgcctttga agtagttagg tctgatcctg atttttcgca
     3601 gataaaaaat gctatcgtaa aaaaaatgta ccatcatcaa tatactaaga aagaggaact
     3661 tattctatat tagtgtctca aataaataaa aaaggatttg actaaatttc cctttatata
     3721 atagagggaa aaaaatacct ttgaggtaga aatgaaaaag ctgagtatac ttttaatgct
     3781 gatcatagcg attggcgttc ttacggcaat caattttgat cccgaactat tctattcgaa
     3841 aacaattatt gcttgtttta cgaaaacagc cattcccaat ttagatggag ttgttaattt
     3901 tgatgtgcaa gatggaatta tttcaacggg gatgagttca tttgaccagt tggcaaagga
     3961 attgcatatt gtggatatga aacagatgca tccttatgta aaagttcctg aatggaacga
     4021 taatggaatt tacctgcaaa atcactaccg tctctattta gaaagtgatg ataatatgga
     4081 taaagcagtg gaacttttat ctaaggaccc gaatttggtt tatgcagaat ttgaaggtat
     4141 caaccgtcct aagtatgtta ctaatgaccc tatgttagat ttacagtatg ctcatacttt
     4201 agtgcatagt tttgaagcat gggattatac acttggtagt catgatgttt tagtggctat
     4261 tacagattct ggagtaaaat ggaatcatcc tgatttgaga gctaatatct ggataaatcc
     4321 tgctgaatct cctggtatga caattgattg gaatgcgggc acaattagtg gtggaaatgg
     4381 tgttgatgca ggagaaggtg gaaataaagt agatgacctt gtaggatggg atttttataa
     4441 taacgataat aatccaatgc aaaatttcgc ggctaatgat catggaaccc atgtagcagg
     4501 ttgtgctgga gccgttggtg ataatgaaat tggcgttgtg ggaacttgtc caaatgtttc
     4561 aattctttgt tgtaaaggat cttctaatac atctccatca actggagttt cctatgctta
     4621 cgatcaagtt aaatatgctg gagaaattgg agctgacata ataaatgcca gttggggtag
     4681 tgtgggaagt ggaacttatc caaattccat agttaactat gtaacagcct taggggcttt
     4741 agttgttacc gctgccggta atgaaaacac agaacataat gcttcctatc aagattatcc
     4801 tgctgattgt acaaacgctt tatgtgttgc tgctgttgct caaggagatg taaaagcttc
     4861 attttcagat tatggatcta caatagatat atgcgctcct ggagcagcaa ttctttctac
     4921 aattattact ggagatggat atgcagctta tgatggaact tctatggcta gtccagttgc
     4981 tgctggtgtt gctgctttag ttaaatctct aaatccaaat ttaacacccg tggaattgat
     5041 gcaacgcttg atggctactg ctgataatat tgatagctta aatccagatt atgttggttt
     5101 gttgggtgct ggcaggataa atgcttatac tgcaactatg tatgataaaa tccccaatat
     5161 gcaaatagag gattatatcc tggaagaaat ttctggtgat gatgatggag tagccaatcc
     5221 tggcgaagtt attaaattga gaattgccct aagcaattgc attgatccta tcacaggtct
     5281 tggttggtta caggcagaaa atgtaacagt tactatgcgt tgccactatc ctggagtaac
     5341 cattattgat tctgtagcta cctttgggac tatgcatgct ggaacaactt tactgaataa
     5401 caatcagcct ttcaagtttc agacagtttc tactttacct tcagaaccaa tacccttcca
     5461 gttttatgtg caggctaatc aagataatca atacccctat aataaagccc ttcctataga
     5521 tatatcacta tctttaaatc aagcaggatg gcctttttat tctggtgcag caacaaattc
     5581 cacccctctt ttaatagata tggatgatga tggtcagaaa gaagttgttt tcgttggagc
     5641 cgatggtaac attgaagttt tacacaaaga tggattaacc agttatcccg gtttccctgt
     5701 tcagacatct tctgttatta ttggttctat agctatggga aatatcaata acgattctaa
     5761 tagagagttt gttgcctgct tgcaaaataa tcaggttatt tgttttaatg acttaggcga
     5821 agtaaaatgg aataatgcat cagggggaac cttacgaaat ggtcctgtta ttttccgtcc
     5881 caatgcagct tccgaattaa aagttgcctg tgttactcaa aacgggattt tgaatatttt
     5941 taatggagat ggtagtaact ttactaattt tcctgtaact gaaaattagt aaagttacta
     6001 ccatctccat taaaaa
//
LOCUS       DAORHB010000077         5975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_13437_length_5975_cov_4.929223, whole genome shotgun sequence.
ACCESSION   DAORHB010000077 DAORHB010000000
VERSION     DAORHB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13437_length_5975_cov_4.929223"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1855
                     /locus_tag="PKM80_03345"
     CDS             98..1855
                     /locus_tag="PKM80_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92973.1"
                     /translation="MSEKKESFTYYLKLLYPFIKKDIGLLLFGLFAMLITSGLRLLHP
                     LILAQIIDHSIPDRNMAQMFQYGGYFVIVVLLSGLLSYLQILLLSRLGIKIITKFKGN
                     VFSHLLKLPVSWFNKQPVGELIARVESDSERVKVLFSDLSITIIGNVLFFIGGFIVLF
                     ILQWQITIYILPAIIICAVGYWFLFKYLNGFFRLIRERYAVITAKITDYVQGIQMLQA
                     LNQEKKALKDVEQASLSKKKVEVHTQMIEYGSQSVFSFMVNVVFIIIVILVSAPKIIA
                     GTLTVGILIVFIQYIYQLVWPLMQISENVMEIQRSFASLKRILELTKLPTEDELYTGT
                     LHPVFEQEIRFENVWFGYKEDEWVLKDVSLTIPKGKKIALVGPSGSGKTTTVSLLCGF
                     YPVQKGRILIDGIPLSEINFREWRRKIGLILQDVYLFPGSILENVRVYNDQIEEAKVQ
                     EAISIVQLNEFIKKLPEGINSELAERGQNISQGEKQLISFARALAFESEIIIMDEATA
                     SIDPQTEAKIQHSMQNLLAGKTALVVAHRLTSVLDADEILYFNEGRITARGKHSELLN
                     NSPEYRKLVELQMLQVQDE"
     gene            1848..3614
                     /locus_tag="PKM80_03350"
     CDS             1848..3614
                     /locus_tag="PKM80_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV92974.1"
                     /translation="MNKHIKWILEQYKAQKWFVLIMVVLTLLSSIVSIAFPYVFKEML
                     DLLKDILQFPDKYPEPMREVYKIIKLLIAIGMAELITGIYPGIRGWVNMRFEHSLRVM
                     YFKHILAKDFSFFQKFKTGDIVTRLTDDLSDYPRVSWFLCSGIFRAFNSFSMILFALV
                     VMFSINIKLTLLSIAPLPIMMLVFYFTADKVYTNYNRNQQAISAINTQLEMSFSGIRI
                     VKSFVSEEKYNRFFDVALAKRFKTEMSMVKLNAILSLIWQYIDYIAQVAVILFGGYLA
                     VKGEITVGTFFLFYTYLSMMIYPLIDLPQLFVTGKQAFVNIDRLEEMKNYPSLNDKFK
                     GEHKVKDFCALSFENVSFQYPDKPFPVLKNASFNLDRGERMLILGPTGSGKTTIANLL
                     LGLLKSDSGTIKINGKPIDEIDIKSLRNIVAYVPQEPLIFGGSIKDNILFAVEKASEK
                     DYHIAVQTSQLENEIASFPERDETFVGNRGLSVSGGQKQRLTIARALVKKPQLLILDD
                     ITASLDAENEEKLWQALDENYPGIAAIVISHRLSTLRYVDSVLFIDSQGNTHKGTHDY
                     LVFNNKEYHDFLREHLKQENKK"
     gene            3675..4772
                     /locus_tag="PKM80_03355"
     CDS             3675..4772
                     /locus_tag="PKM80_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV92975.1"
                     /translation="MRRSFLIILLCAILTFNYADELDDKMRQLREMQKQLESTQQKVK
                     QTQSKKQQTENEIKRTSSLKKRTEANLNKLRLDEKIAQDSLKSVSERIVATEMRIADM
                     HKEQNTELDILLRVDRSYKKQNINHRDQRLLKTLIQGTRQKINILSGFKISLIQVQEL
                     HRKQASKLSRGVKEESYKKTTYEKKIRNLSTQSQKLTIEQKKLQAQVEQLKKDSAELE
                     SLIAQLMKQQGKEPASYQFTGKKIVWPLRGKIIRNYGEETRSYGTSVVSNGIDIAAKE
                     GTKVVAADDGEVIFSDRYGGQGKLIIIDHKNGFFTLYAYNSELLVSRGAKVKKGQTIA
                     KSGMTGSASEPSLHFELRKDGRAINPLPYLE"
     gene            4782..5897
                     /locus_tag="PKM80_03360"
     CDS             4782..5897
                     /locus_tag="PKM80_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNV92976.1"
                     /translation="MLISKDPGLRKLEKFLDSNSLEHSTILHWTGSMWGIGCRLSSLE
                     TIARIETLKQRAEKQGFIALIPDINYLDQKQIPAALRPLMHQYWPGNLTFIFPYQDSK
                     FETITNKGKVAFRVPSDPALRAFISLLGEPLISTSVNVSGLMPEENYDRIISKYGSWF
                     DFALLPANKEIKSTTEPSTIIEYISAADSGNNDEIKCLREGSIPFYEIKQAFKLPVVM
                     FVCTANICRSPMAEYLFRKMARENNISLATDSCGLLPGGQMISVNALQLLLERGILDA
                     QQHISQQITPQLVSSSWLILTMEERQRDLLRTTYPNSESRIMTLNEIVGEKGDIEDPF
                     GRNLESYRKTCEIIENRLQLLTEYLKNNELNIQKKII"
BASE COUNT         1976 a          946 c         1202 g         1851 t
ORIGIN      
        1 cggttcggga ccgagaagtc ggaggttcaa atcctctcat cccgaccatt ttttttaata
       61 tcactaatat cataaatcaa gaaaaaaggt gcaaaaagtg tccgaaaaga aagagtcatt
      121 cacatactat cttaagctac tttatccttt cataaagaag gatataggtt tgctactatt
      181 tgggcttttt gcaatgttaa tcacctctgg tttaaggttg ttgcatcctc taatattagc
      241 tcagattata gaccacagca ttcctgatag aaatatggcg cagatgtttc aatatggggg
      301 ttattttgtt attgtagttt tgctatcagg acttttatcc tatttgcaga tattgttatt
      361 atctcgttta ggcataaaga ttatcacaaa atttaagggc aatgtattta gccatttgtt
      421 aaagctgccg gtttcttggt ttaataaaca accggttggt gaacttattg cccgggtgga
      481 aagtgacagt gaaagagtta aagttctttt ttcagacttg tctatcacta ttattggcaa
      541 tgtgttgttt ttcattggtg gttttatagt tctgttcatt ctgcaatggc agataacgat
      601 ttatatttta cctgcgatta ttatttgtgc agttggctac tggtttttat ttaagtattt
      661 gaacgggttt ttccgtttaa ttcgggaacg ctatgctgta attactgcta aaataactga
      721 ttatgttcag ggaatccaga tgctgcaggc attaaatcag gaaaaaaagg ccttaaaaga
      781 tgtggagcaa gcatctttaa gcaaaaagaa agttgaagtc catactcaga tgattgaata
      841 tggttcgcag agtgttttca gttttatggt aaatgtggta tttataatta ttgtaatact
      901 tgtatctgca cctaaaatta ttgctggaac tttgactgta ggaattctaa ttgtttttat
      961 tcaatacatt tatcaacttg tttggcctct aatgcaaatc tcagaaaatg taatggagat
     1021 tcagcgttct tttgcatctt tgaaaaggat tctggaatta acaaaattgc ctactgagga
     1081 cgaactatat actggaactt tgcatcctgt ttttgaacag gaaatccgtt ttgagaatgt
     1141 atggtttggc tataaagaag atgaatgggt gttaaaagat gtatcgttaa ccattcctaa
     1201 aggcaaaaag attgcattag taggtccttc tggcagcggt aaaacaacaa ctgtaagttt
     1261 attatgcgga ttttatccag ttcagaaagg gagaattttg attgatggca tccctttaag
     1321 tgaaataaat ttcagagaat ggcgtagaaa aatagggctc atattacagg atgtttattt
     1381 gttcccgggc agtattttag aaaatgtgcg cgtatataat gatcagattg aagaagctaa
     1441 ggttcaagaa gctatttcca ttgttcagct aaatgaattt atcaaaaagt tacctgaagg
     1501 tataaactcg gaacttgcag aacgcggaca aaatatatcc caaggggaaa aacagttaat
     1561 cagttttgct cgggctctgg cttttgaatc ggaaataatt attatggatg aagcaactgc
     1621 ttcaattgac cctcaaactg aggcaaaaat ccaacattca atgcaaaatc tactggctgg
     1681 taaaacagcg ctggtggtgg ctcaccgtct tacttctgtt ttggatgcag atgaaattct
     1741 atatttcaat gaaggaagaa ttactgccag gggtaagcat agtgaattat taaataactc
     1801 tccagaatat agaaaactgg tggaattgca aatgctacag gtgcaagatg aataagcata
     1861 taaaatggat tttggaacag tataaagcac agaaatggtt tgtgctaata atggtagtgc
     1921 ttactctgct atcttcaatt gtttcaattg cttttcctta tgtttttaaa gagatgctgg
     1981 acctattgaa agatatattg cagtttcccg ataaatatcc tgaaccgatg cgagaagttt
     2041 ataaaattat caaacttcta attgctatcg gtatggctga gcttataact gggatctatc
     2101 ctggaataag aggttgggtt aatatgcggt ttgaacatag tttaagggtt atgtacttta
     2161 agcatatttt agctaaggac ttcagctttt tccagaagtt taaaactgga gacatagtaa
     2221 cgcgtttaac agatgatctt tctgattatc caagggttag ctggttccta tgttcaggta
     2281 tttttcgcgc tttcaattct ttcagtatga tcttgtttgc gctggttgtt atgttcagta
     2341 tcaatattaa actaaccttg ctatcgattg ctcctttacc aataatgatg ctggttttct
     2401 attttacagc ggataaagta tatacgaatt acaatcgtaa tcagcaagcg atttctgcta
     2461 tcaatactca gctggaaatg agcttttccg gaatcaggat agtgaaaagt ttcgttagcg
     2521 aagaaaagta caatcgcttt tttgatgtag cgcttgccaa gagatttaaa accgaaatga
     2581 gtatggttaa gcttaatgcc attctttctt tgatctggca atatattgat tacattgctc
     2641 aggttgcagt tattctattt ggcggttatt tggctgtgaa aggagaaata acagttggaa
     2701 ctttcttcct gttttacaca tatctttcaa tgatgattta tccgctgatc gatctacctc
     2761 aactttttgt tacgggaaaa caggcctttg tgaatataga ccgcttggaa gagatgaaga
     2821 attatccatc gttaaatgat aaatttaagg gtgagcataa ggtaaaagat ttctgtgcgt
     2881 tatcctttga aaatgtctct tttcaatatc ccgataagcc atttcctgtg ctgaaaaatg
     2941 ccagctttaa tcttgatcga ggagaaagga tgcttatttt aggtcctact ggaagtggta
     3001 aaactactat tgccaatctc ttacttggtc ttttaaaaag cgatagcggg acaattaaaa
     3061 taaatggaaa acctattgat gagatagata taaaatcctt gcgtaatata gtagcctatg
     3121 ttcctcagga accattgata tttggtggat ctataaaaga taatattctt tttgcggtgg
     3181 aaaaagcctc tgaaaaagat taccatattg cagtacaaac atcccaattg gaaaatgaaa
     3241 ttgcctcatt tccggaaaga gacgaaactt ttgtgggaaa tagaggactt agtgtttctg
     3301 gtggtcagaa acaaagatta accattgcca gagcattagt aaaaaaaccg cagttgctta
     3361 ttttggatga tattacggct tccctggatg ctgaaaacga agaaaaactg tggcaagcgc
     3421 tggatgaaaa ttatcctggt atagctgcta ttgtaatatc tcatcggctt tctactttac
     3481 gctatgtaga tagtgttctc tttatcgatt cacagggtaa tactcataaa ggcactcacg
     3541 attatcttgt ttttaataac aaggaatatc acgattttct gcgtgaacat ttgaaacaag
     3601 aaaacaaaaa atagcattga cacaaccgca taatttaaaa aattgtgata aatagattta
     3661 aaagggagat gaagatgcgt cgcagttttc tgattatttt gctatgtgcc atattaacat
     3721 ttaattacgc tgatgaattg gatgataaaa tgcgccagct gagagaaatg caaaagcaac
     3781 tggaaagcac tcagcaaaaa gttaaacaaa cacaaagcaa aaaacaacaa acagaaaatg
     3841 aaataaaaag aacttcatca ttaaaaaaac gcacagaagc taatcttaac aagcttagat
     3901 tagacgaaaa aatcgctcag gattccctta aatctgttag tgaaagaatt gtagctactg
     3961 aaatgcggat agcggatatg cataaagagc aaaatacaga gctggatatc cttttaagag
     4021 tagatagatc ttataagaag caaaatataa atcatcgcga tcagcgattg ctaaaaacac
     4081 ttattcaagg taccaggcaa aagattaata tactttccgg ctttaaaatc tctcttattc
     4141 aggttcagga attacatcga aaacaagcaa gtaaactttc ccgcggagtt aaagaagaaa
     4201 gttataaaaa aacaacatac gaaaaaaaga tacgcaattt atccactcag agccagaaac
     4261 ttactataga acaaaaaaag ctgcaagcac aggtggaaca attgaaaaaa gacagtgcag
     4321 aattggaaag tttgattgct caacttatga aacaacaagg taaagaacct gccagttacc
     4381 aatttacggg taaaaaaatt gtctggccac taagaggaaa gattattaga aattatggag
     4441 aagaaaccag aagttacgga accagcgttg taagtaatgg aattgatatt gctgccaaag
     4501 aagggacaaa agttgtggct gcagatgatg gagaagttat tttttcagat cgctatggtg
     4561 ggcagggaaa gctgatcatt atagatcaca aaaacggctt tttcactctt tatgcttata
     4621 atagcgaatt attagtgagc cgcggagcaa aggttaaaaa aggacaaacc attgctaaaa
     4681 gcggaatgac aggttcggca agtgagccat ctttgcactt tgaattacgc aaggatggaa
     4741 gagccataaa tccattacca tatttggagt aaggaggtat tgtgcttatt tcgaaggatc
     4801 ctggcttacg gaagctggag aaatttttag attcaaattc tttggaacat tctacaattt
     4861 tacattggac tggttcaatg tggggaatcg gatgtcggct ttcctctttg gaaacaattg
     4921 ctcgcattga aacattaaaa cagagagctg aaaaacaagg ttttattgct ctgattcctg
     4981 atattaatta tttagaccag aaacaaatac cagcagcgct gagaccttta atgcatcaat
     5041 attggcctgg gaatttaacc tttattttcc cctatcagga ttctaagttc gaaactatta
     5101 ctaataaggg aaaggttgct tttagggttc cttccgatcc tgctctgaga gcttttatat
     5161 cattattggg tgaaccttta atcagtacca gtgtgaatgt gtctggtctg atgccggaag
     5221 aaaactatga tcgaattatc tctaaatatg gttcttggtt cgattttgct ttattgcccg
     5281 ctaacaagga aataaaaagc acaacagaac cttctacaat tatagaatat atcagtgctg
     5341 cagatagtgg taataacgat gagattaagt gtctgagaga aggctcgata ccgttttatg
     5401 aaattaagca ggcatttaag ctgccagttg ttatgtttgt ttgtacagct aatatatgcc
     5461 gttcaccgat ggctgaatat ctctttcgga aaatggccag agaaaataat atctcccttg
     5521 ctactgattc ctgtggcttg cttccaggag gacagatgat ttctgtaaat gctttacagc
     5581 tgcttttgga acggggaata ttggatgcac aacaacatat ttctcaacaa attactcctc
     5641 aactggtttc ctccagctgg ctgattttaa ctatggaaga aagacagcgt gatttattac
     5701 gcacaaccta tccgaatagt gaaagcagaa ttatgactct taatgaaatt gtcggtgaaa
     5761 aaggagatat tgaggatcct tttggccgaa atttggaaag ctatagaaag acctgtgaga
     5821 ttattgaaaa ccgtttgcaa ctgctaaccg aatatttgaa aaataacgaa ttaaatatac
     5881 agaagaaaat tatatgaaag attatgaaat aacccctaaa gtaatcgctg agcataatct
     5941 cagcgatgaa gaatatgaac gaattacaaa tatcc
//
LOCUS       DAORHB010000078         5901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_13671_length_5901_cov_4.063633, whole genome shotgun sequence.
ACCESSION   DAORHB010000078 DAORHB010000000
VERSION     DAORHB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13671_length_5901_cov_4.063633"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PKM80_03365"
     CDS             complement(<1..1131)
                     /locus_tag="PKM80_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV92977.1"
                     /translation="MDKLQTDSRNKKGIMRSVKSIFGINSSMLGMLIMVILVGMGEKM
                     AERFLPLYLIAMGGTSIIIGLLNGMDNLLSALYSFPGGWLADKIGYKKALNIFNLMAM
                     FGYLIVIIFPSWIAVLVGAVFFISWTAISLPATMSLISSVVPVNRRSMGISMNSIVKR
                     IPMSLGPILGGALILRYGRIQGVRYAFIVAFILAGVALLVQSKFITEQPKKEKTEVSK
                     SSRRFSANLRHLLVSDILVRFCEQIPYAFVVVWVVENNHINELQFGILTTIEMVTAIL
                     IYIPIAYLADRSSRKPFIAITFANFTLFPIVLYFCHNFTSMIFAFIIRGLKEFGEPTR
                     KALIMDLAPEDAKASIFGTYYMIRDVIVSFAAFGGAFLWERNP"
     gene            complement(1286..1567)
                     /locus_tag="PKM80_03370"
     CDS             complement(1286..1567)
                     /locus_tag="PKM80_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNV92978.1"
                     /translation="MSRDELVKKIAASAGISQKVASAALNAVLEGITESLKKGDKVSL
                     VGFGSFSVAHRKARNGINPKTKKPLKIPARNVPVFKAGKNLKEAVKKAK"
     gene            1868..3055
                     /locus_tag="PKM80_03375"
     CDS             1868..3055
                     /locus_tag="PKM80_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV92979.1"
                     /translation="MKCKIMTFIVLTLLVLLSACSGNKKVEMQTEQVPVRNPLVLAHQ
                     AATSGTDLYNKELYNDAIKSFSEAIDLFTEASATASAVDSIAENIEKMKLNIAKSYIE
                     LASESTEMNMYEDALVFYNKALNIYTSIAPTLISEEELNQNIIGLYNNMAIVAKQAGK
                     YENALEYYNQILKLNPKDTDTIYAKFYVLKDNLNDENRAYNVLIEYAELTRDAKAFIN
                     IGDFYADKGNMIEASKYYQKALAIEPNVDVYRRLANYYRITKDWANANVYLLKVVESN
                     PEPSELAQIYIMIGKNYDEMKDKKKMVEYYEKSLSIERDPQLALLLASYYNSQKNYAK
                     VISNANITLSIDPKNVDALMLRGVAYFQTKNYTAAKADLERLKNDPKYGAQATNLLKN
                     IPK"
     gene            3112..3555
                     /gene="umuD"
                     /locus_tag="PKM80_03380"
     CDS             3112..3555
                     /gene="umuD"
                     /locus_tag="PKM80_03380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNV92980.1"
                     /translation="MKLHSDGSIKAIYTAEQGKKQYRPLMSSKIAAGFPSPAQDYIEG
                     KLDLNEHLITHPASTFFVRVDGYSMQGAGILPDDILIVDRALEAVNNNIVIAIVDGEL
                     TVKRLIIKGNDYYLMPENDEFEPIQIQEGMDFVIWGVVTFVIHKV"
     gene            3560..>5901
                     /locus_tag="PKM80_03385"
     CDS             3560..>5901
                     /locus_tag="PKM80_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92981.1"
                     /translation="MASIKPCIFLLLFLSGITYLFAQSLYEQHNLVLEPNQQKYTLTN
                     SPIIKNSVCVWADTLLLKEGIDYIVDHKKAELILLYEPEAPVLKVEYLQIPSSLTKPL
                     QLWEPRLYSDSLLSNIKKRSNPVFSSDARLNIQGAKTFSISFSDDSAFDLKQSLYVNL
                     SGELANNVYINAQLSDSQSKLSPEGDSKELSSLDNVFIRIYGPKYELAMGDLEIKYTG
                     TRYMEYYSKFEGINAWVKGKHYAQTAFSAGGGKNASVKINIIEGKQGPYYLKANDTQS
                     NFLVLAGSEEIFIDGNKLERGLDYSIDYSEGSVMFKRLVTASNTVIARFQYSDEFYPQ
                     SSYLNSTKVQLSEHLSFSHHFIWQQDDKKNPLIYEFSTADIDSLQAAGDNQIWGEGVL
                     TVEAGTGNYKQLLNSQNEYYYEYAPGDSLACYNIIFSFVGSGNGDYEEYSLGKYRYIG
                     QGLGSWLPQKRLIPPEKKGNLDMAFAYSNEGLNAGIEVLGTVNDQNTLSIKDDNDNNA
                     GLIYAYLEIPYSLYSLKLDYEQRSEQTYLFGKYRNPNEEYDFASIETADSLAQHESNI
                     QLSRQQPNWNSSLLFRYRKIYDLYSLQAFRFISSSSGKGFLPAINIRSTISKQDYQKD
                     DDLNGTMQYHQGDISWFIYNIRLHFETLYNLLDTSTFGNSYLKYSPGITIGKASSFLT
                     QLSHSSDITKLNDNDHWNIISETQTYALKQIVNLNSQRLDLDVTHRVVKQPYSTSNPE
                     SNYDLITFRSSNSFLKNAIGLYSNYQVNQTEFYPKIRELQ"
BASE COUNT         2020 a          998 c         1057 g         1826 t
ORIGIN      
        1 tggatttcgt tcccacaaaa aagctcctcc aaaagctgca aaagaaacaa tcacatcccg
       61 aatcatataa taagtaccaa aaatcgatgc tttggcatct tctggagcaa gatccataat
      121 taaggctttt cttgtaggtt cgccaaattc tttcaagccc ctaataataa aggcaaaaat
      181 catagaggtg aaattatggc agaagtataa tactataggg aaaagtgtga aattggcaaa
      241 agtaatagcg ataaaaggtt tgcgactact tcgatcagcc aaataggcaa ttgggatata
      301 aattaaaata gcggttacca tttcgatagt ggttaagatt ccaaattgta attcattgat
      361 atgattattt tctactaccc aaacaactac aaatgcataa ggaatctgtt cgcaaaaacg
      421 gactaaaata tcggaaacaa gcaaatgacg caagttagca gaaaaccgac gggaactttt
      481 actaacttca gttttctcct ttttcggctg ttcagtgata aatttacttt gaaccaataa
      541 agctacacct gctaaaataa aggcaacgat aaaagcataa cggactccct gaattcttcc
      601 atagcgcaaa ataagagcac ctcccaaaat tggacctaat gacataggaa tgcgttttac
      661 aatggaattc atagaaatcc ccatagaacg acgattcaca ggaactacag aagaaatcag
      721 actcatcgtg gcaggtaggg aaatagctgt ccaggaaata aaaaagaccg ctcccactaa
      781 aacagctatc caggaaggga aaatgatcac aataagatat ccaaacatag ccatcagatt
      841 gaatatgttt agagcttttt tgtaacctat tttatcagcc aaccatcctc ccggaaatga
      901 atataaggca cttaataaat tatccattcc atttaaaaga ccgataataa tggaagtgcc
      961 tcccatagct attaaatata aagggagaaa acgttctgcc attttttctc ccattcctac
     1021 cagaataacc attatcagca ttcccaacat actactgtta attccgaaga tagattttac
     1081 gctacgcatt atccctttct tgtttctact gtctgtttgt agtttgtcca tttagttgtc
     1141 ccttttcaat ttagtaggtt attgaaaaaa tatgtgctca aagataaaat atagaagaaa
     1201 gcaaaaatac caagtgtaat tttataggtt aatacaaaag gcagaaagcc aaaaggcttc
     1261 ctgcccattt tgtaaagtaa ctgaattatt tggctttttt aactgcctct ttaaggtttt
     1321 tacctgcttt gaagaccgga acattgcgag ccggaatctt caaaggtttt ttggttttag
     1381 ggttaatacc attgcgtgct ttacgatggg ctacgctgaa tgaaccaaaa ccaaccaatg
     1441 aaactttatc cccttttttc agggattcgg taatgccctc gagaactgcg ttgagggctg
     1501 cactggcaac tttttgtgaa ataccagcac tagctgcgat tttttttacc aattcgtctc
     1561 tgctcatttt acctccttat ttggttctct tgtaaagagt gagacacctt acaatcgtta
     1621 ttaaagtgca ctatgatatt tttgtcaata aaaataatta catttatcgt atttgcaacg
     1681 attttatgtg gtttgactcc tgcactctaa gaagataatt atatatgata taaggagtta
     1741 caagaacgac actaaatgca ataattttct tgatatttat ctattttata gctttttact
     1801 tttttaagtt gacaaaaatc agagagctaa gtagagagga tatcaaataa taatggagga
     1861 tttaccgatg aaatgtaaaa taatgacttt catagttctc actcttttag tactacttag
     1921 tgcttgcagt gggaacaaaa aagtggagat gcaaactgaa caagttcctg tgcgtaatcc
     1981 tttagtatta gctcatcaag ctgctacttc aggaacagat ttatataaca aagagctata
     2041 taatgatgca ataaaatcat tttcagaagc aattgattta tttacagaag catctgcaac
     2101 tgcttctgct gtggattcaa tagctgaaaa tattgaaaaa atgaaattga acattgcaaa
     2161 atcctatatt gagcttgctt cagaaagcac agaaatgaat atgtatgagg atgcgttggt
     2221 tttttataat aaagctctta acatctatac ttctatagct cctactttaa tttcggagga
     2281 agaattaaat cagaatatta ttggtcttta caacaatatg gctattgtag ctaaacaagc
     2341 tggtaaatat gaaaatgcgt tggaatatta taatcagatt ctaaaactga atcctaaaga
     2401 tactgatact atctatgcta agttttatgt tttgaaagat aatttgaacg atgaaaaccg
     2461 tgcttataat gtccttatcg agtatgcaga actaactagg gatgctaaag cattcatcaa
     2521 tattggggat ttttacgctg ataaagggaa tatgattgaa gcaagtaaat attatcaaaa
     2581 agctttagct attgaaccta atgttgatgt ttaccgtcgt ttggcaaatt attatcgtat
     2641 cacaaaagat tgggcaaatg ctaatgtcta tctgctgaaa gttgtagaat ccaatccgga
     2701 accatctgag ctggcacaga tttatattat gatcgggaaa aactatgacg aaatgaagga
     2761 caagaaaaaa atggttgaat attatgaaaa gtccttaagt atcgaaagag atccgcagct
     2821 tgctctatta ctggcaagtt actacaattc tcagaaaaat tatgccaagg ttattagcaa
     2881 tgccaatatt acgctaagca tagatcctaa aaatgtagat gctttaatgc tgaggggagt
     2941 tgcttatttt caaactaaga attatacagc agccaaagct gatttggaac gccttaaaaa
     3001 cgatcctaaa tatggtgctc aggcaacaaa tctacttaag aacattccta aataataaac
     3061 tagcattcaa ggacgggtta atcaatatac ccgtcctgct ctttataaat tatgaaacta
     3121 catagtgatg gttcgataaa agctatttac acagctgagc aagggaaaaa acaatatcgt
     3181 cctttaatga gttcgaaaat tgcagctggc tttccatctc cagctcagga ttatattgaa
     3241 ggaaaacttg atcttaacga acaccttata acccatcctg cctccacttt ttttgttaga
     3301 gtagatggat attctatgca aggagcagga atattaccgg atgatatact aatcgtggat
     3361 agagcattag aagcggtgaa taataacata gtaatagcaa ttgtagatgg agaacttacg
     3421 gttaaacgtt tgataataaa ggggaatgat tactatttaa tgcccgaaaa tgatgaattt
     3481 gaaccgattc agattcaaga gggtatggat tttgtgattt ggggtgttgt aaccttcgta
     3541 atccataaag tataatcagt tggcatctat taagccctgt atttttttat tactttttct
     3601 ttcagggatc acttatctct ttgctcaatc tctgtatgaa caacataatc ttgtgctcga
     3661 gccaaaccaa cagaaataca cacttaccaa ttcacctatt atcaaaaaca gcgtttgtgt
     3721 ttgggctgat accctgttgc tgaaagaggg aattgattat atagtagatc ataaaaaagc
     3781 ggaattgatt ttattgtatg agcctgaagc tcctgtttta aaagtagagt atttacaaat
     3841 tccatcttct ttaacaaaac ctttacaatt atgggaacca cgcctttata gtgatagttt
     3901 attatctaat ataaaaaaaa gaagcaatcc tgtatttagc agtgatgctc gtctgaatat
     3961 tcaaggggct aaaaccttct ctataagctt ttctgatgat tcggcttttg atttgaaaca
     4021 atcactttat gtaaacctct ctggagaatt ggcaaataat gtttatatca atgctcagct
     4081 ttcggatagc cagtccaaac tatctccaga aggcgattcg aaagagctta gttcattgga
     4141 taatgtattt atcagaattt atggaccgaa atatgaactg gcaatgggtg atctggaaat
     4201 aaaatataca ggaactcgct atatggagta ttatagtaaa tttgaaggta tcaatgcctg
     4261 ggtgaaagga aaacattatg cccaaactgc attttctgct ggaggaggca aaaatgcgtc
     4321 tgttaaaatc aatatcatag aaggaaagca aggaccatac tacctaaaag caaatgatac
     4381 tcaatccaat tttctagttt tggctgggag tgaagagatc tttattgatg gtaacaaatt
     4441 agagcgggga ttagattata gtattgacta tagtgaaggt agtgtaatgt ttaaacgctt
     4501 ggtaacggct tccaatacag ttatagctcg atttcagtat tctgatgaat tttatccgca
     4561 aagtagttat cttaattcta cgaaagtgca actttcagaa catctttcat tttctcatca
     4621 ttttatctgg cagcaggatg acaaaaaaaa tcctctaatc tatgaattca gtactgctga
     4681 tatagattct ttacaggcag ctggagataa ccagatttgg ggtgaaggag ttttgacagt
     4741 cgaggcagga acaggtaatt ataaacagtt actaaattct caaaacgaat attattacga
     4801 atatgcaccg ggggattctc ttgcttgtta caatatcatc tttagttttg tgggtagcgg
     4861 aaatggagat tatgaagaat attctcttgg aaaatatcgt tacataggac aaggattggg
     4921 gtcttggctt ccgcagaaac gattaattcc acctgaaaaa aaagggaatt tagatatggc
     4981 atttgcctat tccaatgaag gtcttaatgc aggaatagaa gtattgggaa ctgtgaatga
     5041 tcagaatact ttatcaataa aggatgataa cgataataat gcaggtctga tttatgctta
     5101 cttggagatt ccatactcac tatattcttt aaaattggat tatgaacagc gaagtgaaca
     5161 aacatacctc tttggaaaat accggaatcc gaacgaggaa tatgatttcg cttccataga
     5221 aacagcggat tctttagccc agcacgaaag caatattcaa ttaagccgtc agcaacctaa
     5281 ttggaatagt tcattactat ttcgatatcg aaagatatat gatctttatt cgttacaggc
     5341 atttcgtttt atttcttcat cttcaggaaa gggttttttg cctgctataa atataaggag
     5401 tacgatttcc aagcaagatt atcaaaagga tgacgattta aatggaacta tgcaatacca
     5461 tcagggagat atatcctggt tcatatataa tatccgtctg cattttgaaa ctctgtataa
     5521 cctgttagat acaagtacat ttggtaacag ttatttgaaa tatagtcctg gaattacgat
     5581 tggtaaggca tcctcttttc ttactcaact atctcatagc tctgatataa cgaaactgaa
     5641 tgataatgat cattggaaca taatttctga aactcaaact tatgctttaa agcagattgt
     5701 aaacctaaat tcacaacgcc ttgatttaga tgttactcat cgtgtagtga aacagccata
     5761 ttctacttct aatcccgaat ccaattatga tctaatcact tttcgttcca gcaacagctt
     5821 tctcaaaaat gctattggac tatatagcaa ctatcaagta aatcaaactg agttttatcc
     5881 taaaatccgt gaattgcaat a
//
LOCUS       DAORHB010000079         5845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_13860_length_5845_cov_4.200518, whole genome shotgun sequence.
ACCESSION   DAORHB010000079 DAORHB010000000
VERSION     DAORHB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13860_length_5845_cov_4.200518"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="PKM80_03390"
     CDS             <1..1750
                     /locus_tag="PKM80_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV92982.1"
                     /translation="LFKLTAASSQINNFSDLPVPFICNATDLITGEGVTFTKGSLMQA
                     LRASISIPSLFEPFELNGHLYIDGGISQNMPINLLHEMGADKVLGIKVNSTLRKEENL
                     NNFVDILDQTINIGITRNLNEHLDVCDLLIEPDLTNFASTDYQHIQSIIAAGEESARQ
                     MIDQIRAFKKELSLSPERIINFDKHLDNFPVANIFVYGNNTISAAKVREYLGLETGHT
                     YTSEQIKKACINAWNSQSFRTIYPVLEKRNDDKYNLLVYVKEREHKHLAVNLVYNNDE
                     QLMAGLVLSLDNYLLKNSRLLGELKLGGRNEFNLDYVKNFGEQWGAYYRLFPYVTEKT
                     IYNYRDHYKTDSVKSTEWGINSGVGVFAKDLAIAELFIYSSQTNLHSDISQTPFLPKR
                     SVISGFGVKGYHESLDDYSFPTSGARVFGKFNFSRYGEVSDYLYSCFQGRAEFYVPIM
                     NLFSVYSEFNYGSYLNSSQQTNLDPFVISGIDGFMGYSRYEVSAPHYQIISAGISSLI
                     SKRFFLDIGIQSLRYSDQNIFENPEDWEYCGYLGFGYKNSILPVKLYVSLNEDKDLHS
                     FLSVGYDFDIFFFSRR"
     gene            complement(1938..3308)
                     /gene="glmM"
                     /locus_tag="PKM80_03395"
     CDS             complement(1938..3308)
                     /gene="glmM"
                     /locus_tag="PKM80_03395"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNV92983.1"
                     /translation="MNKLMTSVSGVRGVFADTLNPIIAIKYAAHFALIQKRHYPEEKP
                     HIIVGRDSRTTGQAMLHSIISAIISVGCDVTDLGIVSTPTLLLKVQESDAIGGIAITA
                     SHNPPQWNAMKFVDADGMFLSPEKAEYFLNSVENEIIWAAWDSMGKITKDNSAIQEHI
                     DRILAIPYLNLEQIRSRKFKVVLDSVNGAGGLISPYLLEGLGCTVYEINSEATGIFAH
                     TAEPLNENLHQLEEAVTFYKADIGFATDPDVDRLSLVSEQGKCIGEELSVALAELFVL
                     PKKRGDIVVNLSSSMISDDIAELFGVQVHRTKVGEINVGKKMQAIKSPIGGEGNGGII
                     CPDVHYTRDAIAGMALILGLLAEQDKPLSAIVDSLPKYYFAKDKLILDASRMENVMQN
                     IPSFFEDYQLDTQDGIKAIGNKHFIHIRKSGTEPIIRIYVESESIEKSRALCEEAKKI
                     IERASI"
     gene            complement(3312..5096)
                     /locus_tag="PKM80_03400"
     CDS             complement(3312..5096)
                     /locus_tag="PKM80_03400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV92984.1"
                     /translation="MLDQLRKKQKVIIYIVAFVFIVGMGAIGLGELLSPKPYLGKVNG
                     TKITFDMFQQKTQEMYDRYAQMYKDQPIDENTRKAIENQAWESLVNDILWQQQIKKHK
                     IKITDDEILTEMQNNPPQELMQNEALQTNGRFDRSKYLSALKNNPQFFAAMEEYVRSY
                     LPRQKLQNKIKAQAGITIDSLKAEYAKDSDIVSGKFIWFDPNKSDPVTVTDAEIKAYY
                     DKNKETEFKKGPASRMKYIAFEEKPSDKDYASIKLLADEVYNRVIKGEDFATLAKEYS
                     DDPGSKDNGGSLGEFGKGQMVPEFEKVAFSLKVKEISKPVKTNFGWHIIRCDSIASQD
                     PENYRIKASHILLKVDPSDETKNALLDKVESARALIKKKGIDAAAKELKVEVQKTDWV
                     PHDQEYIPGIGNNGALLQFMRKGKEKAVSDIITDQQNRKIIAQLTDNKKVYYEDFESV
                     KLRIKYQLEKEKKVANIKKKAEEFVAKYPSGEYFSAAAKEGWQIIDLNNHKKGQYVPN
                     VGLCEEFTNAALALEAGKTSGLIHTKEGSFIISCSERIKPDFNAFAKDKDKQEEIRKR
                     LEDAAWNRWYDQMRKEAKIIDNRAKYGM"
BASE COUNT         1774 a         1096 c          992 g         1983 t
ORIGIN      
        1 gctttttaaa ttgacagcag catcctctca aataaacaat tttagcgatt tacctgtgcc
       61 ttttatttgt aatgctaccg atcttataac gggtgaaggt gttactttta caaaaggatc
      121 tttgatgcag gctttgcgcg cttccatatc aattccaagt ttgtttgaac cctttgaact
      181 aaatggtcac ctatatattg atggaggtat ttctcaaaat atgccaatta acctcctcca
      241 cgaaatggga gcggataaag ttttgggaat taaagttaat tctactttgc gcaaggaaga
      301 aaacctgaat aactttgtgg atattttgga tcaaactatc aacatcggca ttacccgtaa
      361 tttgaacgaa cacttagatg tatgtgatct tcttattgaa ccagatttaa ctaattttgc
      421 ttccactgat tatcaacata tacagagtat catagctgcc ggagaagaat cagcaaggca
      481 aatgattgat caaatacggg ctttcaaaaa agagctatct ctttctcctg agcggataat
      541 taattttgat aaacatctgg ataattttcc agttgccaat atatttgtgt atggaaataa
      601 tacaattagc gctgctaaag tgagagaata tttgggcttg gaaacagggc atacctatac
      661 aagcgaacaa ataaaaaaag cttgcatcaa tgcctggaat tcccaaagtt ttagaacaat
      721 ttacccagtg ctggaaaaaa gaaacgatga taaatacaat cttcttgttt atgtaaaaga
      781 acgcgaacat aaacacctgg ctgtgaatct tgtatacaat aatgatgaac aactaatggc
      841 tggtttggtg ctttctttag ataactattt gctaaagaac tcccgacttt taggagaatt
      901 gaaactgggt ggcaggaatg aatttaatct ggattatgtt aaaaattttg gagaacaatg
      961 gggggcatat tatcgacttt tcccatatgt aacagaaaaa acaatatata attaccgtga
     1021 tcattataaa accgatagcg taaaatctac tgaatggggt ataaattcgg gagtgggtgt
     1081 ttttgctaaa gacctcgcca ttgctgaatt attcatttat agtagtcaaa ctaatctcca
     1141 tagtgatatc tctcaaacac cctttcttcc taaaagatcc gtaataagtg gatttggcgt
     1201 gaagggttat cacgaaagtt tggatgatta tagtttccca acttccggtg ccagagtttt
     1261 cggcaaattc aatttctctc gatatggaga agttagtgat tatctgtata gttgttttca
     1321 aggaagggca gaattttatg ttcctattat gaatctattc tctgtttatt cagaattcaa
     1381 ttatggcagc tatctaaatt cttctcaaca aactaatctc gatccttttg tgataagtgg
     1441 aatagatggt tttatgggat attcacgcta tgaagtaagt gctcctcatt accaaattat
     1501 atctgctggt atttcctctc ttatctcaaa acgcttcttt ctggatatag gaatccagag
     1561 tttacgatat tctgatcaga atatttttga gaaccctgaa gattgggaat actgcggata
     1621 tttaggattt ggttacaaaa attccatatt accagtaaaa ttatatgtct cgctaaatga
     1681 agataaggat ttgcatagtt ttctaagcgt gggatacgat tttgatattt tcttcttctc
     1741 cagaagataa aacaagataa ctttttacag atttctcaga ttcggtacac ccgaaatgaa
     1801 tagaatgaaa ggattggctg gttttatgaa ttgtaggttg tctggagaac tatacagtta
     1861 aagcaaatcg cattatatac tttcctgaat gacgataaaa cagctggtta tactatactt
     1921 taattatttc gttctcttta tatagaagct ctttcgatta tttttttggc ttcctcgcag
     1981 agggctcggg atttctctat tgattcactt tccacataaa tacggataat tggttcagtt
     2041 cccgatttgc gaatatggat aaaatgttta tttccaatag ctttaatccc atcctgagta
     2101 tccaattgat agtcctcaaa aaaggacggt atattctgca tcacattttc catacgggag
     2161 gcatccaaaa ttagtttatc cttggcaaaa taatatttgg gtaagctatc aactatagca
     2221 gaaagcggtt tatcctgttc ggcaagcaaa cctaaaatca atgccatacc agcaatagca
     2281 tcacgcgtat aatgaacatc aggacaaata attccaccat ttccttctcc gccaatgggt
     2341 gattttattg cctgcatctt ttttcccaca tttatttcac caacttttgt tcgatgaacc
     2401 tgcacgccaa aaagctcagc tatatcatcc gatatcattg aactgctaag attaactaca
     2461 atatctcccc gttttttggg tagtacaaaa agctctgcca aagctacgct taattcttct
     2521 ccgatacatt ttccttgttc agaaacaagg gaaaggcgat ctacatcagg atctgtagca
     2581 aagccgatat ctgctttgta aaaagtaact gcttcttcca attggtgtaa attttcattc
     2641 aaaggttcgg ctgtatgggc aaaaatcccg gttgcttcac tatttatttc atatacagtg
     2701 catcccaaac cttctaatag atatggagaa attaaacctc cggcaccatt tacggaatct
     2761 aacacaactt taaatttacg ggagcgaatc tgttctaaat taagatatgg gatagccaaa
     2821 attctatcta tatgctcttg aatagcactg ttatcctttg ttatttttcc catagaatcc
     2881 caggcagccc agataatttc attttcaacg gaattcaaaa aatactcggc tttttcaggg
     2941 ctcaaaaaca ttccatcggc atctacaaat ttcatagcat tccactgagg aggattatga
     3001 gatgcagtaa tagcaatgcc tccaatagca tctgattcct gaacttttaa tagcaaagta
     3061 ggtgtagata ctattcctaa atcagttaca tcacaaccca cacttatgat ggcagaaatt
     3121 atgctatgca acatagcttg tccggttgtg cgactatctc ttccaacgat aatatgaggt
     3181 ttttcttcag gataatgccg tttttgaatt aaagcaaaat gtgctgcata ttttatggct
     3241 ataataggat taagggtgtc cgcaaagaca cccctaaccc cactaacact ggtcatcaat
     3301 ttattcatta atcacattcc gtatttggca cggttatcga ttattttagc ttcttttcgc
     3361 atttggtcat accaacggtt ccaagcagca tcttccagac gcttacgaat ttcttcctgt
     3421 ttatctttat ctttagcgaa agcattaaaa tcgggcttaa ttcgctctga acaactgata
     3481 ataaagcttc cttctttagt atgaatcaaa ccgctggttt ttccagcttc caatgctaaa
     3541 gcagcattgg taaattcttc gcaaaggcca acattgggaa catattgtcc tttcttgtgg
     3601 ttgttcaaat ctattatctg ccagccctct ttagctgcag cagagaaata ctcgccagaa
     3661 ggatacttgg caacgaattc ttctgctttt ttcttaatat tagctacttt cttttccttt
     3721 tccagctggt atttaatacg aagcttaacc gattcaaaat cttcgtaata gacctttttg
     3781 ttatcagtga gctgagcgat aattttacgg ttttgctggt ctgtaataat gtccgataca
     3841 gctttctctt tacctttacg cataaactgt aaaagagctc cattatttcc aattccagga
     3901 atatattcct gatcgtgagg cacccaatca gttttttgaa cttcaacttt cagttctttt
     3961 gcagcggcat ctattccttt tttctttatt aaagctctag ccgattcaac tttatccagc
     4021 agcgcatttt tggtctcatc tgagggatca acttttagca aaatatggct ggctttaatt
     4081 ctgtaatttt caggatcttg ggaggcaatg ctatcacaac ggataatatg ccaaccaaaa
     4141 ttggttttaa cgggcttact gatttctttt actttcagag aaaaagctac cttttcaaat
     4201 tcaggaacca tttgcccctt tccaaattcg cctaaagaac caccattatc tttagaaccg
     4261 ggatcatcgg aatattcctt ggctaaagta gcaaaatcct ctcctttaat aacacggtta
     4321 taaacttcat cggccaataa ttttatagag gcatagtcct tgtctgaggg cttttcttcg
     4381 aaagcaatat atttcattct cgaagctggt ccttttttaa attctgtttc tttgttctta
     4441 tcataatagg ctttaatttc agcatcggta acggttactg gatctgattt attgggatca
     4501 aaccaaataa attttccgga aacaatatca gaatccttcg catattctgc ttttagacta
     4561 tcaatagtaa ttccagcttg agcttttatc ttattttgca atttttgacg aggaagataa
     4621 ctacgcacat attcttccat agcagcaaaa aactgaggat tattcttcaa agcagaaaga
     4681 tatttggatc tatcaaatct gccattggtt tgtaaggctt cattttgcat cagttcctga
     4741 ggtggattat tctgcatttc ggttaaaatc tcatcatcag taattttaat tttatgcttc
     4801 tttatctgct gttgccacaa aatatcgttc accaaagatt cccaagcttg attttctatt
     4861 gctttacggg tattttcgtc tataggttgg tccttataca tttgggcata gcgatcatac
     4921 atttcctgtg ttttctgttg gaacatatca aatgttattt tggtaccgtt cacttttccc
     4981 aggtaaggtt ttggcgatag taattcccct aacccaattg cacccatacc tacaataaac
     5041 acgaaagcaa caatgtagat aatcactttc tgtttctttc tcaactgatc cagcatttcc
     5101 attctccttt tgtagaggat aaatttctta ttttgaaagc cattatttta atagtgcagt
     5161 ttttaacaag gttttttttg cacttctgcc aatactgtgt tttctattcc ctgttaagat
     5221 atttattagt agcgcaatta tataatctat aatattgtta tctttattat cctacgggga
     5281 tgagagcata attacgattg gtttagaaca ctgtgtatga ggttaataaa gaagctcatt
     5341 tagaaatatc agaatgaagc ttataaaaaa atattcaggt ttcctgataa aacagttata
     5401 ccataatagt tttgtatgtt ctgtttatat cttgaaaatt tctccccagg ccagttattc
     5461 ggttcaataa ttctatttta gttaaagtag aattattgac ccaacacaaa ttcaaaattt
     5521 gcagataaat aatgatagaa gatcattctc tagcttcaag ataaggcaaa atattttatc
     5581 ctttaagatc gattattcga agctattaag atttaattag accaagaata tcagcaaaat
     5641 acaaagatta tttctatcta aaccttactt tctcttgcaa tttaacaata tacaacttgt
     5701 catccaccca agcttttctc atccttaacc atgggttgaa gaggagagga ggatgagagg
     5761 aggatgggtt gaagtgtcct aaaaagaatg gaactggagt tcaatgaaga taagaccagg
     5821 ctggtcaaag tggagagtga tccct
//
LOCUS       DAORHB010000080         5782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_14099_length_5782_cov_4.760258, whole genome shotgun sequence.
ACCESSION   DAORHB010000080 DAORHB010000000
VERSION     DAORHB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14099_length_5782_cov_4.760258"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..953
                     /gene="era"
                     /locus_tag="PKM80_03405"
     CDS             30..953
                     /gene="era"
                     /locus_tag="PKM80_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNV92985.1"
                     /translation="MGWFSTPFLFYMSISANFKSGFVAIIGKPNTGKSTLMNCILGEK
                     ISITSPKPQTTRYAIKGIWNTPEHQIVFVDTPGYLKPRYELQERMLKIWHNSLEDVDL
                     VLFLTQVDSFPTEYDKEVLHHLQYLKNPQLAVFNKLDLNPEVNREDMKQYLPDSINKA
                     FFVSAKTGENIPELMEAITYYMPYHEPYYEEDMLSDLPMRFFAKEIIREAIFHYFQEE
                     IPYSSAVLIERFQELPDKVIIHAVIWLERKSQKLILIGKNGSALQKIREYAEVELSRF
                     MQLPVYVHLFVKINPKWRKKDNALRELGFMP"
     gene            complement(995..>5782)
                     /locus_tag="PKM80_03410"
     CDS             complement(995..>5782)
                     /locus_tag="PKM80_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92986.1"
                     /translation="VFGEISTYNDYLRLNANNSPDGIINGDDATVNLTNGWIIMPFIE
                     PMQGLGDGIIYRKENEDEYSYQDSTSIFINIKGKIGREAIELSSMGVLKGSVRVRVNG
                     VEQRENTDYIVDYDFGRITFLTAAGKDPDAKIEIDFESRTLFSVAQKNLAGIRANWQM
                     NDYTKLGGTLIYRSESVADKRPRIGNENIEMIMGDIDGEVTIKPEFITRGLDALPLIN
                     TTAPSQISMSGEIAFTLPNIYGDPDSKKKEAYLDDMESIMDSYPLGVTYNNWILGSKP
                     FGTSYAKGRINWFNPKDVKRRDIEAEATLTDQEKDEYVTVLTMIVKPNPVQIPNSTTH
                     SWAGIMKYLGNQLDFSQKKYLEVYAKLEPINPNTNIYPNVTMHIDLGDINEDFYTEFG
                     GYGVLNSEDVNRDGVLTISEDIGLDGISKNQPGHDPNDIAYPASGNDYSGVNGTEDNR
                     ILDTEDLDGNGVLNTLDRYISYSFSLSNPDTSIVIDSYNQWRMYRIPLSDEQYFQLVN
                     SSGTNIQPSLQKVSYARIWLDTDCTMDIKVRIADISVVGNKWQDNYIRQFKYSSAPPP
                     NSYVQYYNLMIDPTVLAAKNTSFISGIVSNQKNFSHYTSPPGTWYTEENRESAESALT
                     LEVKHLQRDQMCLLRQKMLDTQNLLSYDKIRFWVYPEAAQGSYSHLDSLYVIFRIGAD
                     SLNYYQVRQRVPVHPYNNKISAKDWIQLEYNLQDLISLKEINPNADADSLVNIVPGDE
                     GKIGKYYRGKPSLINIREIYLGVYVPGTHFYAAEEEIKEFSGTIYFDDIRVADPYEDI
                     GIAKRLSLSGTFADVATLNIDYEEKSENFNTTMQRGRSNTFTSVRSLNVTNKIFVNKL
                     LPNSWNMDIPISLNRNYSLGIPRFRANSDLLRANMADPELKEIEKTEHLAYSADFGLS
                     QKQPPKSKILQYTIYRTSISGRIENSYNNTSTAIDTLLAYRGTLNYNLNLPADKTSLK
                     LFNDYRLGYFPNTFSNSFTFSSNEPKSWDRVTTVDSLAWNKRSQTTDTRTITTDNNIS
                     WNILSDVTATARLNTKRDLKQKDYIYDINVGKQTEYVQDLGLNYSPNYLPRIFNFTSS
                     ASARYTDTQRKYTQYIGGSAVDTYQRDGNTNRSIRMNLTLMNSSLFTEWTTKMMSKMP
                     EGTISPKVKDKDKYPKSEMSEEEIKKQEEHQKEEEIKKQEEHQKEEEIKKQEEHQKEE
                     EIKKQEEHQKEEEIKKEELKPEEEQKLSEEEINKRQDEIARLKAEGKFDQLSEEEINE
                     LLADISAGKGKKETQENDGEKEPEANGPGFNPLLTFVSALSHIKNINASYQNTYVMNY
                     ARKTNPFPFAFQLGIPHCVPYDSLEAISNDNTLTIGSGITFSRRLDSVINCSVTSNRR
                     YASASNQTIGYTFPDITLSLMDFESIVGLQKYITGSRLNTGFQYTVRQNGNLDWVKPK
                     QESYTTALNPLLGFTGNLFKVVSTNLSYSISQTDNITDMDTYEILKTSNSQTLNGNLS
                     YSFRAARGFSVPFTKKKIHIKNELTSSLGITYESNYDKTKGSSSSQVDRNTSRFSIIP
                     QATYQFDANIRGGLTGRYEVNSDKKTEDGTSIFGLGIWVEVNL"
BASE COUNT         1729 a         1036 c          981 g         2036 t
ORIGIN      
        1 tgaaagtgaa atataacaat aaatagctta tggggtggtt ctccacccct tttttattct
       61 atatgagtat ttcggcaaat tttaaaagtg gctttgtagc tattataggg aaacctaata
      121 caggtaaatc taccctgatg aactgtatct taggggaaaa aatatctata acatcaccca
      181 aacctcaaac tacgcgttat gctattaaag ggatatggaa tactcctgag catcagattg
      241 tttttgtgga tacacctggt tacttaaaac ctcgttatga gcttcaagaa aggatgttga
      301 aaatttggca taactcttta gaagatgtag atttagttct tttcctcact caagttgata
      361 gcttccctac agaatatgac aaagaagtac ttcatcatct tcaatattta aaaaatccac
      421 aactagctgt atttaacaaa cttgatctca atccagaggt aaatagagaa gatatgaaac
      481 aatatcttcc ggattcaata aataaagcat tcttcgtgtc ggccaaaaca ggagaaaata
      541 ttcctgaatt aatggaagca attacatatt atatgcctta ccatgaaccc tattatgagg
      601 aggatatgct ctctgatttg cctatgcgtt tttttgccaa ggaaattatc cgtgaggcaa
      661 tcttccatta ttttcaagaa gaaatacctt attcttcagc ggtactaatt gaacgctttc
      721 aggaattacc ggataaagtt atcattcatg cagttatctg gttggaacgg aaaagtcaga
      781 aactaattct gataggtaaa aatggatctg ctctacaaaa aattcgagag tatgctgaag
      841 ttgaattatc tcgctttatg caattacctg tatatgtaca tctatttgta aaaatcaatc
      901 caaaatggcg aaagaaggat aatgccttaa gagaacttgg ctttatgcca taaataatta
      961 tatttcgttt atttccatct tggctttttc tattctatag atttacttca acccatattc
     1021 ctaatccgaa aatggaagta ccgtcttcgg tttttttatc ggaattaact tcatacctac
     1081 ctgttaaacc acctctgata ttagcatcaa attgataagt cgcttgtggt ataattgaaa
     1141 agcggcttgt gttacgatca acttgtgatg acgaactacc tttggtttta tcatagttac
     1201 tttcataggt tatccccaaa gaggaggtta gttcattttt tatatggatt ttcttttttg
     1261 taaaggggac agaaaagccg cgtgcagctc taaaactata agaaagatta ccatttaagg
     1321 tttggctgtt actggttttc aagatttcat aagtatccat atctgttata ttatcagttt
     1381 ggctaataga gtagctaagg ttagtagata ctactttaaa gagatttcca gtaaaaccaa
     1441 gtaaaggatt caaagctgtt gtataggatt cttgtttggg cttaacccaa tccaaatttc
     1501 cattttggcg aactgtgtat tggaaacctg tattcaatct ggaaccagtg atgtattttt
     1561 gtaaaccaac aattgattca aaatccatta aagataaagt aatatcagga aaagtataac
     1621 caatggtttg attacttgca ctcgcataac gtctattgga agtaacagag cagtttataa
     1681 cactatctaa tctgcgagag aaagttatcc cactacctat agtaagagta ttatcattgg
     1741 aaattgcttc cagagaatca tacggaacac aatgtggaat tcctaattgg aaagcaaaag
     1801 ggaagggatt tgttttacgc gcataattca taacatatgt gttttgatag gaagcattaa
     1861 tgttcttaat atgcgaaaga gcgctaacaa aagtaagtag aggattaaat cccggtccgt
     1921 tagcttcagg ttctttctct ccatcatttt cttgggtttc ttttttacct tttccagcag
     1981 atatgtctgc aagcagttca ttaatttctt cttcagatag ctgatcaaat ttgccttcag
     2041 ctttcaaacg ggcaatttca tcttggcgtt tatttatttc ttcttcgcta agtttttgtt
     2101 cctcttctgg cttcagttct tcttttttaa tctcttcttc tttctgatgt tcttcctgtt
     2161 ttttaatctc ttcttctttt tgatgttctt cctgtttttt aatctcctct tctttctgat
     2221 gttcttcttg tttttttatt tcttcttctt tctgatgttc ttcttgtttt tttatttctt
     2281 cttcagacat ttcggattta gggtacttat ctttgtcttt gactttgggg gagatggttc
     2341 cttcaggcat tttactcatc atttttgtag tccattctgt aaacagagag gaattcatca
     2401 aagttagatt catacgaatg gagcggtttg tattaccatc tctttgatat gtgtctactg
     2461 ctgaaccacc aatatattga gtatattttc gctgtgtatc tgtatatcta gctgaagcag
     2521 aagaagtaaa gttaaaaatg cgtggcaggt aatttggact gtagttcaat cctagatctt
     2581 gcacatattc tgtttgttta cctacattta tatcgtaaat atagtctttt tgtttcaggt
     2641 ctctttttgt gtttagacga gctgtagcag ttacatcgct taatatgttc caggaaatat
     2701 tattatcggt tgttatagtt ctggtatcag ttgtctggct acgtttattc caagccaaac
     2761 tatctacagt agtaacccta tcccagcttt taggttcgtt actactaaag gtaaaactat
     2821 tgctgaaggt gtttgggaaa taacctaatc ggtaatcatt gaacaatttc aagctagttt
     2881 tatcagcagg aagattcaaa ttatagttca aagttccccg atatgccaaa agagtatcta
     2941 ttgcagttga agtattatta taggagtttt caattctacc gctaatagaa gttcggtata
     3001 tagtatattg cagaattttg ctttttggtg gttgtttttg gctgagcccg aaatctgcac
     3061 tgtaagctaa atgttccgtc ttttcaattt cttttagctc aggatcagcc atattagctc
     3121 ttaaaaggtc tgaatttgca cgaaaacgag gaattcctag cgaataatta cgatttaagg
     3181 aaataggaat atccatattc caagaattgg ggagcagctt attaacaaat attttatttg
     3241 ttacattgag agatcggaca ctggtaaaag tattgctgcg tccgcgttgc atcgtagtat
     3301 taaagttttc gctcttttct tcataatcta tatttaatgt ggctacatca gcaaaagtcc
     3361 cgctaagact aagacgtttt gcaattccta tatcttcgta cggatcagca actcttatat
     3421 catcaaaata aatagtcccg ctgaactctt ttatttcctc ttcggcagca taaaaatgag
     3481 ttccgggaac ataaacaccc agataaattt ctctgatatt tattaagctg ggtttacctc
     3541 tatagtattt tcctattttt ccttcatcac caggaacaat attaaccagg gaatccgcat
     3601 ctgcatttgg gttaatttct tttaaggata taaggtcctg caaattatat tcaagctgaa
     3661 tccaatcttt ggcacttatt ttattattat agggatgaac tggcactcgt tgacgcactt
     3721 gataatagtt taaagaatca gcacctattc ggaaaatgac atataaagaa tctagatgtg
     3781 aatatgaacc ttgagcagct tcaggatata cccagaaacg gattttatcg taagagagca
     3841 aattttgggt atccagcatt ttttgtctga gtaaacacat ttggtcgcgt tgaagatgtt
     3901 taacttctaa ggttagagct gattctgccg attctcgatt ttcttctgta taccaagttc
     3961 ccggaggaga agtatagtga gagaagttct tttgattact tacaattcca gagataaaac
     4021 tagtattttt agctgccaaa acagtaggat ctatcataag attataatat tgaacataac
     4081 tgtttggtgg aggtgcacta ctatatttga attgacgaat ataattgtct tgccatttat
     4141 ttccaactac agaaatatct gcaattctaa cttttatatc catagtacaa tctgtatcca
     4201 accagatacg tgcatacgaa actttttgta aacttggttg aatatttgtt cctgagctat
     4261 ttacaagttg aaagtattgt tcatcactta atggtatacg atacattcgc cactgattat
     4321 aggaatctat tactatagaa gtatcgggat tagagagaga aaaagaataa cttatgtaac
     4381 gatctaaggt atttaaaact ccgttaccat caaggtcttc agtatctaat atgcggttat
     4441 cttctgttcc attcactcca gaataatcat tcccactggc aggataggca atatcattgg
     4501 gatcgtgacc aggttggttt ttcgaaatac cgtctaaacc aatatcttcg ctgatagtta
     4561 aaactccatc acggtttaca tcttcgctgt ttaaaactcc ataaccacca aattcagtgt
     4621 aaaaatcctc attaatatct cccaagtcta tatgcattgt tacatttggg taaatatttg
     4681 tgttaggatt tataggctcc agtttggcat acacttccag atattttttc tgagagaaat
     4741 cgagctggtt accaagatat ttcataattc cagcccagga atgtgttgta gaatttggga
     4801 tttgcacagg attgggtttt acaatcatag taagcacagt tacatattca tctttttctt
     4861 gatctgttaa agtagcttca gcctcaatat cgcgtctttt tacatctttg ggattgaacc
     4921 agtttattct tcctttggca taagaagttc caaaaggctt actacccagt atccaattat
     4981 tataagtaac tcctaaagga taagaatcca taattgattc catatcatcc agatatgctt
     5041 cttttttctt actatcagga tctccgtata tattaggcag cgtaaaagca atttccccag
     5101 acatcgatat ttgggaagga gcagtagtat taattaaagg aagggcatct aaacccctag
     5161 taataaattc tggtttgata gtaacttcgc catctatatc gcccattatc atttcaatgt
     5221 tctcattacc aattcgaggt cgtttatctg ctacactctc acttcggtaa attaaagttc
     5281 cccccagttt ggtataatca ttcatttgcc agtttgctct aattcctgcc aagttttttt
     5341 gagctacact aaaaagagta cgagattcaa aatctatttc tattttggca tcaggatctt
     5401 tacccgcagc agttaagaag gttattctgc caaaatcgta atcaacaata taatcagtgt
     5461 tttcgcgttg ttctacaccg tttactctaa ctctaacgct tccctttaac actcccatag
     5521 aagaaagttc tatagcttct cgtccaattt tacctttaat attaataaaa atactggttg
     5581 aatcctgata ggaatattca tcttcatttt ctttacggta gattatacca tctcccaaac
     5641 cttgcatcgg ttcgataaaa ggcataataa tccaaccatt ggtaagattg acagtagcat
     5701 catcaccatt gataatgcca tcgggactat tattagcatt taacctcaga tagtcattat
     5761 aggttgatat ttcaccaaaa ac
//
LOCUS       DAORHB010000081         5754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_14214_length_5754_cov_4.376206, whole genome shotgun sequence.
ACCESSION   DAORHB010000081 DAORHB010000000
VERSION     DAORHB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14214_length_5754_cov_4.376206"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKM80_03415"
     CDS             <1..426
                     /locus_tag="PKM80_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92987.1"
                     /translation="ELYLRILNDPNSDYDPRVEELFAPEELAYLRIMNHQGFNNLAQA
                     RAKLEEGMQGSEIIFSQVMPEDVLSDESEVEVLTSILQEIEQQAQLNIVSEKSDFDEY
                     NVQDLLIAILAKFDKNQKLSEVKLSDLVTLFLEMQSLKT"
     gene            608..1156
                     /gene="rsmD"
                     /locus_tag="PKM80_03420"
     CDS             608..1156
                     /gene="rsmD"
                     /locus_tag="PKM80_03420"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNV92988.1"
                     /translation="MRIITGIYKGRNLFLVPGITTRPTTSFNREVIFSVFQSYQGCRV
                     LDLFAGTGSFGFEALSRGAKWVDFVEFAPAAVNTILKNIALLGCSDNCHLWRKKVDVY
                     LKSCKEKYDIIFLDPPYNKNLINPSLKLIYENELLNESGILIVEHSPKENIEVPYSDF
                     INTEKYFKNTNFSWLDSKLYPK"
     gene            1762..4221
                     /gene="sppA"
                     /locus_tag="PKM80_03425"
     CDS             1762..4221
                     /gene="sppA"
                     /locus_tag="PKM80_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNV92989.1"
                     /translation="MRSFSTIFTVLLLLLLSSVAIAQNVSNNNSVLLSDQDFPIANVD
                     NMFIPVSNPSLIGTGTSSGVGLAYLNDEKKWQDHYWLFVNTDFLSYIYEKDHSDNFHT
                     LALGTELFPAFILPNLYVGTNYRWLEDDFENGIFRSGITYRPHNSTSVAFTWDNPKHN
                     SPYYRLGLAFRPFTFSEAIADYRLELSLDANYVRYENDNDYEIKKPVIGLQTQILDGV
                     KIGATYNLDKETAFVNFSLCAANLEAGSLLHSKDNDNYGIAWAQLTSLNYKPFLGYKD
                     SYWYKMDLTGNVVTYAAPKFKIGPFSLYDTKDKSIETIINEINKAKEDPNVQGILLKN
                     PSFSTSLALQEELIATFLDFKSTGKQVCLYYDNIGNAGYMFAAAIADKIYLNPMGSVD
                     LRGLSISSPYIKNMLSSLGIEVLNFRSHKYKNAGNMFSEERMTDAEKEVYDSILQSLY
                     AQILQRIEVGRGDKLTASPDEIINAGPYFIATEALDNGLVDALIYEDQLNKQMKKDFK
                     FVKKQKELTDYRDYAWSKPKENQIAVIYASGNIVSGKGTPGQKIAQETTVNLIRKARY
                     NNMYKGIILKVDSGGGSAQASDIILRELELAQTENKKPVVVSMGGTAASGGYYISCNA
                     DKIIAEPSTLTGSIGVVGLVFNGTEMFNKIKVNWDTVKKGEHSDIGSFNRPWTDEEKE
                     MMTRSIEDVYETFVKRVDIGRSSLDYNQVNEYAQGRVWTGAQAYEIGLIDGLGGMETA
                     KENMQQLLGKKGKITLVDATTKKEGVKISFNISEMNTFTPLKAYNAVNNDYIKLYELW
                     EDYAGDKALMISPELPEILQF"
     gene            complement(4540..5316)
                     /locus_tag="PKM80_03430"
     CDS             complement(4540..5316)
                     /locus_tag="PKM80_03430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV92990.1"
                     /translation="MEYEPLKNLLENVISVFPCLRKLLYLQFNIIFLRQRYVFREIRN
                     LFHNTDKIKLYDAGAGFCQYSYFVLSYWPESTAFATDLKTNYLRSFANYAADKYPGRF
                     FYKSADLQIFKPANKYDLALAIDILEHIEDDESAIKNLKEALKPGGYLIISTPSDLDE
                     AAKFTSEHIRAGYHKEDLEEKLQQAGFSIIKSIFTYGTFGSLSWKLMLKLPMQITSKK
                     LYIILPIYYLLIYPVCEILMRIDMKTDNKKGTGILIVAQS"
     gene            5394..>5754
                     /locus_tag="PKM80_03435"
     CDS             5394..>5754
                     /locus_tag="PKM80_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNV92991.1"
                     /translation="MIVKEINDIKRINLHLHTNVSDGSLSPKTLVNKAREIGLDLISI
                     TDHDTADAYAQIPENVSPLRILPGVEISSRYEDDDVHILAYGCDLTNKALQEMMEMYL
                     VGRKERAKKMISILADMG"
BASE COUNT         1968 a          902 c         1050 g         1834 t
ORIGIN      
        1 gagctatatc tgcgtatttt aaatgatcct aatagcgatt acgatcccag agtggaagaa
       61 ctttttgctc ctgaagaact tgcttatctt agaattatga atcatcaggg ctttaataat
      121 ttagctcagg caagagcaaa acttgaagaa ggtatgcagg gttcagagat aatctttagc
      181 caagttatgc ctgaagatgt tttatccgat gaatctgaag tcgaggtatt aacttcaatt
      241 cttcaagaaa ttgaacagca ggctcagttg aatattgttt cggaaaagag tgattttgat
      301 gagtacaatg ttcaagatct tctcatagcg attttagcca aattcgataa aaatcagaaa
      361 cttagtgagg tcaaattgag tgacctcgtt actttatttc tggagatgca gagtctaaaa
      421 acataattta aatgtttaca aaacttccaa attcagtgaa ttctctttgt gctaactgct
      481 acaataaatg caaacaaaat gattttgttt caattattat ttgccctttt tacgatccga
      541 agccagtgca aatggaattg atatttaaaa aaactcgtaa acaacccaag aagaaagcaa
      601 caaaaaaatg agaataataa ccggtattta caaaggacgc aatttatttc ttgtacctgg
      661 aataactact aggcctacaa cttcttttaa tcgcgaagtt attttcagtg tgtttcaaag
      721 ctatcaagga tgcagagttt tagacctttt tgccgggaca ggttcttttg gttttgaggc
      781 tctttctcgc ggagcaaaat gggtcgattt tgttgagttt gctcctgctg cagtaaatac
      841 aattctcaaa aatatagctc ttttaggatg ctccgataat tgtcatctat ggcgaaagaa
      901 agtggatgtg tatttgaaat cctgtaaaga gaaatacgac ataatttttc ttgatccacc
      961 atataacaag aaccttatca acccatcatt aaaacttatc tatgaaaatg aactgcttaa
     1021 tgaaagtggt atcttaattg tagaacattc tcctaaggaa aatatcgaag ttccttattc
     1081 tgattttata aatactgaaa aatactttaa aaacaccaat tttagctggt tagatagtaa
     1141 gctctatcca aaataacaat cttctaagaa gatttttcct gttaacctaa acatatcttg
     1201 actggggtgc ataggaaaaa gtacttcctt ctttaataag ttcagttact aaggaagaag
     1261 taatattctg tttctacaat ttaccaggta tttactactt cagcgtaaat tgcatattgt
     1321 gtgcaagttc cgctcttcgc ccattataga aagcgttaat caagtgttaa tctaacctta
     1381 atcaagcgtt aattattaac gcttgattaa ggtatgttaa acgatagatg aatggattta
     1441 agcaaggtat ataggagata acaatattgg gattttatga aaaaagtgat tgctaaggtt
     1501 ggtataatac ggtttctata gggaaataat cgcagcattt ttctatttta actttcaata
     1561 catccaattg gaattgatac ttatatctat cttataggaa tgaaaactta agaggggaaa
     1621 ctttttacta ttacctctca taaatatatc ttgactaatt accaaggtta taaacaaaag
     1681 tattaaagtt aagaaaaatg gttaccacat caaagcctat tatatttatc agaatagaat
     1741 aatatatttc aggagaaaac aatgcgaagc tttagtacga tctttacagt attgttactt
     1801 ttgctattaa gctctgtagc aatagcacag aatgtttcca acaataattc tgtcttgttg
     1861 agtgaccaag atttccctat tgccaatgta gataatatgt tcattcctgt cagcaatcct
     1921 tctttgatag ggactggaac atcaagtgga gttggacttg cttatctaaa cgatgaaaaa
     1981 aagtggcagg atcactactg gttatttgtg aatacggatt tccttagtta catctatgaa
     2041 aaggatcata gtgataattt tcacaccttg gctttaggta cggaactttt tcctgccttc
     2101 attttaccca atttatatgt aggaacaaat taccgctggc tggaggatga ttttgaaaac
     2161 ggaattttta gaagtggaat aacatacaga ccgcataatt ctacttctgt tgcttttact
     2221 tgggataatc ccaaacataa ttctccttat tatagattgg gtcttgcttt tcgtcctttt
     2281 actttttcag aggcaattgc cgattatcgt ttagaacttt ccttagatgc aaattatgtt
     2341 cgatacgaaa atgataacga ttatgaaatt aaaaagccgg ttatcggatt gcaaactcaa
     2401 atattagacg gtgtgaaaat tggagccaca tataatttgg ataaagaaac tgcgtttgtc
     2461 aatttcagtt tatgtgcagc caatttggaa gcaggcagtc ttttacattc caaagataat
     2521 gataattatg gaattgcctg ggctcagctt acctctttaa attacaagcc atttttaggt
     2581 tataaagatt cttactggta caaaatggat ttaacaggaa atgttgtaac ctatgcagct
     2641 cctaaattta aaattgggcc ttttagttta tacgatacga aagataaatc tatcgaaacg
     2701 ataattaatg aaataaacaa ggcaaaagaa gatcctaatg tgcaaggaat tttgttaaaa
     2761 aatccatcgt tttctacttc gctggctctt caagaagaat tgatagccac ttttttggat
     2821 tttaaaagca ccggcaagca agtgtgcttg tattatgata atattggcaa tgctggatat
     2881 atgtttgctg cagcaattgc tgataagata tacttaaatc ctatgggttc tgtcgattta
     2941 cgaggtttaa gcattagcag tccatacatc aaaaatatgc tttccagttt aggtatagaa
     3001 gttcttaatt ttcgcagcca taaatataaa aatgcaggta atatgttctc cgaagaaaga
     3061 atgacagatg cagaaaagga agtttatgat tccattctgc aatccctcta cgctcaaatt
     3121 ttacagcgga tagaagttgg acggggagat aaattaaccg cttctcccga tgaaattatt
     3181 aatgcaggac cctattttat agcaactgaa gcattagata atggtttggt agatgctctt
     3241 atttatgaag accagctaaa caaacaaatg aaaaaagatt ttaaatttgt taaaaaacaa
     3301 aaagaactaa cagactatcg tgattatgct tggtcaaaac caaaggaaaa tcaaattgca
     3361 gttatttatg ccagcgggaa tatagtttct ggaaaaggaa caccaggaca aaaaatagct
     3421 caagaaacta ctgtaaatct aatccgtaaa gcccgctata ataatatgta taaaggaatt
     3481 atcttaaaag tagatagcgg tggaggaagt gcacaagcca gcgatattat tttaagagaa
     3541 ttggaacttg ctcaaacgga aaacaaaaaa cctgtagttg tttctatggg tgggacagca
     3601 gcaagtggtg gttattacat ttcttgtaat gcagataaga taatagctga accatcaaca
     3661 ttaactggtt ctatcggagt tgtgggttta gtattcaatg gcaccgaaat gtttaataaa
     3721 attaaagtaa attgggatac cgtaaaaaaa ggtgaacatt cggatattgg atcatttaac
     3781 cgtccctgga ctgatgaaga aaaagaaatg atgactcgca gcattgaaga tgtttatgag
     3841 acctttgtaa aaagagtaga tattggacgc tcctctttgg attataatca agtgaatgaa
     3901 tatgcccaag gtcgtgtttg gacaggtgca caagcttatg aaataggttt gatagatggt
     3961 ctgggtggaa tggaaactgc taaagaaaat atgcaacaat tgcttggtaa aaaaggtaaa
     4021 atcaccttgg ttgatgccac cactaaaaaa gaaggcgtaa aaattagttt caatatcagc
     4081 gagatgaata cctttactcc tttaaaggca tataatgccg ttaataacga ttatataaaa
     4141 ctgtatgaac tttgggaaga ttatgctggc gacaaggctc taatgatctc tccagaacta
     4201 cccgaaatat tgcaatttta acttgtttta agataaaggg gttagcggtc gatattgtcc
     4261 gttaaccctt tttttctcat agtttttatc cttacaattc tgaagcattt ctttaacgct
     4321 tccttaatct cgttaaggaa gtgttaaagg atcattaggg aattgcttaa gttacaatga
     4381 attgtccttt tggattataa catactacat tgttggaaat gaagttatta taaacaaggt
     4441 atcacttaag accattgctt gtaatagcat acaattttgc taatatctta tttggaatac
     4501 acttacctga aatccttatt aaaaaaaggc tacacactat tagctttggg caacgatcag
     4561 aattcccgtt ccctttttgt tatctgtttt catatctata cgcatcaata tttcacaaac
     4621 aggataaatg agcaggtaat agataggtaa gataatatac agctttttgg aagtgatctg
     4681 cattggcaat tttaacatca atttccagga taaggagcca aaggttccat aggtgaaaat
     4741 acttttaatt atactgaatc ctgcttgctg aagtttttct tccaaatcct ctttatggta
     4801 accggcacga atatgttcgg aagtaaattt ggctgcttca tcaaggtctg aaggagtgga
     4861 aataattaaa tatccacctg gcttaagcgc ttctttcaaa tttttgatag ctgattcatc
     4921 atcttctata tgctccaaaa tatcaattgc taaagccaaa tcatatttat tggcaggttt
     4981 gaaaatctgt aaatcggcac ttttataaaa aaatcttcca ggatatttat cagcagcata
     5041 gttagcaaaa gatctcagat aattagtttt taggtctgta gcaaaagcag tagattctgg
     5101 ccaataggaa agaacaaaat aggaatattg acaaaaaccc gctccggcat catatagttt
     5161 tattttatca gtattatgaa aaagatttcg tatctctcta aatacatatc tctggcgcag
     5221 aaaaatgata ttaaactgta aataaagcag ttttcgcaaa cagggaaaca cagaaataac
     5281 attttccaga aggtttttca aaggctcgta ttccataact gaatctcctt aaacaaaaaa
     5341 tacattgact atttttagca atgccaaaat aagtcaactg agaaatttag attatgatag
     5401 taaaagaaat aaatgatatt aaacggatca atctgcattt gcatactaat gtttcagatg
     5461 gaagtttgag ccccaaaaca ctggttaata aggctcggga aattggtctt gatctgattt
     5521 ccataaccga tcatgatact gcagatgctt atgctcaaat cccggaaaat gtttcgccct
     5581 tgagaattct acctggtgtg gaaataagtt ctcggtatga agatgatgat gttcatattt
     5641 tagcttatgg ctgtgacctt accaataaag cgttgcagga gatgatggaa atgtatttgg
     5701 tcggccgaaa agaaagagct aaaaaaatga ttagtatttt agctgatatg ggga
//
LOCUS       DAORHB010000082         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_14393_length_5705_cov_4.039115, whole genome shotgun sequence.
ACCESSION   DAORHB010000082 DAORHB010000000
VERSION     DAORHB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14393_length_5705_cov_4.039115"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1041)
                     /locus_tag="PKM80_03440"
     CDS             complement(310..1041)
                     /locus_tag="PKM80_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV92992.1"
                     /translation="MKKLSLLVLSLSLLLGSCNFFTPTERNTPIICLTFDDQRIGVYT
                     YALPEMNIYGYRGTCYVNSFYMSTRYSMNLAQVQSLHNDYNWEIGGHSLKHEDLAELT
                     YDEVEYSINQDYWNLYYWGLNPRTFAMPTGKCPLEYYPILTTHYKYLRGCNDYAMHIP
                     LNIQGLGYLAFQSSWTADIYKERIRRGIANGEDLIILGFHAIEEPDNIYGTNCPLREF
                     KEILRYINHLGLEVLPLYEAVEELN"
     gene            complement(1038..2105)
                     /locus_tag="PKM80_03445"
     CDS             complement(1038..2105)
                     /locus_tag="PKM80_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV92993.1"
                     /translation="MNRIMRNYHSHRERFSMENKIAKMLVVTLLVCCCVTLSAADVPF
                     VLSWKSINSGAYCPNQAKKHLHWNEFYQIGFGVDSLAYKDLYATLELRSRANFYYNNV
                     EIYNCDFVYNKSNWQIMAGSLPWGYGLPNALYTYALVAADQDQFSYQATRLNRVSLGH
                     YFSNHLLSLAIGGNNHNQSIEVLTYQVIKPFGSLSISEEIRAQDNHWKTPVSISSINS
                     QIDKGNIALNAQIAVSYLPKYENTEQHTTSYGLLAVNYKPNDLTELFLSTEYIEVEPS
                     LNDEQRYHIGISRKIDDFFLNPFYYLDYYDNSSSHRAGLSLDYLFHKNQRVGLVYYLE
                     GSKINKAKHNFAIQAQLGIKA"
     gene            2358..3557
                     /gene="coaBC"
                     /locus_tag="PKM80_03450"
     CDS             2358..3557
                     /gene="coaBC"
                     /locus_tag="PKM80_03450"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNV92994.1"
                     /translation="MQGKNILLCVSGGISAYKAIDLASRLCKQGFEVKCVLSENATKF
                     VSPLNFSAITHNSVHTSLFTDADPIPHITLATWADLLIVAPVTANILAKAANGIADDL
                     LSTILVAYDKPVLFVPAMNVKMYHSAIVQENIERLLERGHYILQPATGKLACGTEGEG
                     KYPPNEEIIYAIQTYLNYTLDLKGIKVLINAGATVEAIDPMRFLSNRSSGKMGLAIAR
                     ALALRGAEVTLLYGNLQVELPYNIQDKVFCESADTMFRETIKRYPEMDWIICCAAVAD
                     YKPKEINTQKLPKLSQLNLELISTPDILFELGKLKKENQRLIGFAAQSDNLIRSALDK
                     YYRKNLDLICANDIRYAGRDEDEIIIQGKIKGYPPLKEDPNINSVVMKGDKFSLAHNL
                     INIIKEL"
     gene            3554..4297
                     /locus_tag="PKM80_03455"
     CDS             3554..4297
                     /locus_tag="PKM80_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV92995.1"
                     /translation="MRQILISGANSQVGSYLAKEYYKEGFQLVLLYHNRNERIAEQLG
                     KEGIFSCSVDLCDATAVQAAISESVKQLKQNSDILIHCAAVRSSDALPLFQTEPFQFT
                     YVLKSNIISAYNVLRYSLPPMQEKRFGRVIMFGSDVSRKGLKNGSAYAASKAAIANMV
                     KSVALELAKDNILINTVSPAPIKTALEEDYSGEYLQFRQRYFADYLTNVPTGKFVTKE
                     EIKKVIDLLISEDLQNLTGEEIFIDGGSK"
     gene            4294..5061
                     /locus_tag="PKM80_03460"
     CDS             4294..5061
                     /locus_tag="PKM80_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNV92996.1"
                     /translation="MKAKLGLTCLLLVLTLNLIAAAVPFRDGEKLTFTVKYGIVSAGE
                     ASLQARSSVYQGSPVWYLSTTAKTYPFFDKVYKVRDRVESWWDKDTLLPYKFSKNLQE
                     GNYRQHRVHIYNHQEKSSTYQKWSFKKSVFENTEMVIPEGTQDILSAFYLMRTKKLEV
                     GKSVMVNITADGRNMPTEVVVHRKEKQKTIFGNVECLVIEPKLKGEAIFKQSGRILIW
                     LTNDDYKIPVRLESKITIGSFVATLTSAQQVPFKIQL"
     gene            5058..>5705
                     /locus_tag="PKM80_03465"
     CDS             5058..>5705
                     /locus_tag="PKM80_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNV92997.1"
                     /translation="MIPDLLATNSYDYYLPKELIAQYPAEQRTASRLMHIKRNTGEIA
                     HRQFTDILELIKEGDLVILNSSKVFPARLFAEKENGTKVEILLLYQLNDNKWKCMASP
                     GKRLKQPQYLHFSENLSGFISSGDREGLREIDFTCQINFWEEIQRIGHIPLPPYIERN
                     DTFSDRERYQTVYAKEIGSIAAPTAGFHFDNDILDALRDKGVLFSDVILHIGIGTF"
BASE COUNT         1915 a          967 c         1051 g         1772 t
ORIGIN      
        1 ctgaccttca ccttcggagg gtgccactct atccagctga gctacaggga cgcataaaat
       61 actgatccat aaattctgga aagcagtttt ctgtcaattg agaaagtgag aagcaagaat
      121 ctttatcact ttgagcataa tttcagctta gtacaaaaaa gaactcgtag accttatcag
      181 aaatgcaata ttagcgatgt tttaatatat gcttattatg attatattta gcagccaagt
      241 aaattaagtg ccactcctat tattacaagt gcagatcact cttatctaac tatctgataa
      301 ttagcataat taattcagct cttctacagc ttcataaaga ggtaaaactt ccaatccaag
      361 atgatttata tagcgtaata tttctttaaa ttctctcaag ggacagtttg ttccataaat
      421 attatccggc tcttcgatag catgaaaacc cagaattatt aaatcctcac cattagcaat
      481 tccgcgtctg atacgctctt tgtagatatc agcagtccaa gaactttgaa aagcaagata
      541 acctaaaccc tgaatattta gaggaatatg catagcataa tcattacaac ctcgaaggta
      601 cttataatgt gtagttaaaa taggataata ctccaaggga cattttcctg tcggcatagc
      661 aaaggtgcgg ggatttaagc cccaataata cagattccaa taatcttgat taatagaata
      721 ttccacttca tcgtaagtta attctgccaa atcctcatgt ttaagagagt gccctccaat
      781 ttcccaatta taatcattat gcaaagattg aacctgtgct aaattcattg aatagcgtgt
      841 agacatataa aaagaattca catagcaagt tcctctataa ccatagatat tcatttcagg
      901 taaagcataa gtataaactc caattctttg atcatcaaag gttaagcaaa taatcggagt
      961 atttcgttcc gtgggagtaa aaaagttgca agaaccaagc agcaaagata gtgataagac
     1021 caataatgaa agctttttca tgcctttatt cccaattgtg cctgaatggc aaaattatgt
     1081 tttgccttgt ttattttact cccttccaga taataaacta agcccaccct ctggtttttg
     1141 tggaaaagat aatctagcga taaaccagct cggtgggaag aactattatc ataatagtca
     1201 agatagtaaa atggattaag gaaaaaatca tcaattttac ggctaatgcc aatatggtaa
     1261 cgctgttcat cattaagtga tggttctact tctatatatt ctgtgctaag aaataattcc
     1321 gttaaatcgt tgggtttgta atttacagcc agaagaccat aagaagtagt atgttgctct
     1381 gtgttttcat attttggaag ataactaaca gcaatttgcg catttaaggc aatgttcccc
     1441 ttatcgattt gagagtttat agaacttatg gatacagggg tcttccaatg attatcctgt
     1501 gcccttattt cctcactgat agaaagagag ccaaaaggtt ttatcacttg gtatgtgaga
     1561 acttctatgg attgattatg attgtttcct ccaatggcaa gagataagag atgattagaa
     1621 aaataatgtc ccaaactaac tctgtttaat cgagttgcct gataactgaa ctgatcttgg
     1681 tcagctgcaa caagagcata tgtataaagg gcattgggta aaccgtaacc ccaaggaaga
     1741 gaacctgcca taatttgcca attgctttta ttgtaaacaa aatcacaatt ataaatctct
     1801 acattgttat aataaaaatt agctctgctg cgaagctcaa gagttgcata taagtcctta
     1861 taggcaaggg aatcaactcc gaatccaatt tggtaaaatt cattccaatg caggtgcttt
     1921 ttggcttgat ttgggcagta tgctcctgaa tttattgatt tccaacttaa tacgaaaggc
     1981 acatctgcag ctgagagagt aacgcaacaa catactaata aagtaacaac cagcattttg
     2041 gctatcttat tttccataga gaaacgctct ctatgcgaat ggtagtttct cattatgcga
     2101 ttcatcttca atgcaatgat tcctctttta ttagtaaaac tctttataaa aaggttaatg
     2161 cttactgtca attacttttt tccttgaggt ctgaggtttt tatttttgat tatcctgcca
     2221 atcttcatat tacatagcta agtctcccta tataagttag ttagctctcc actatttttt
     2281 ttaaaactgt ttttatgatt gacataaata ccatagataa atttaaagta taagctaata
     2341 acatctaaag aggaaaaatg caaggcaaga acatcctact ctgtgtaagt ggaggcatat
     2401 cagcttacaa agctatagat ttagcaagcc gtttatgtaa acaaggcttt gaagtgaagt
     2461 gtgttttatc tgaaaacgct actaaatttg tgtccccact aaatttctca gctattactc
     2521 acaatagtgt tcatacctct ctatttacag atgcagatcc tattcctcat attacacttg
     2581 ctacctgggc agatttatta attgtggcac ctgtaactgc caatatatta gccaaagcag
     2641 caaacggaat agcggatgat ttgctttcaa caatattagt ggcatatgac aaaccggttc
     2701 tttttgttcc tgcaatgaat gtaaaaatgt atcattcagc tatagttcag gaaaacatcg
     2761 aaagattact tgaacggggg cattatattt tgcaaccagc aacaggtaaa cttgcttgtg
     2821 gaacagaagg agaaggaaaa tatcctccta atgaagagat aatttatgca atacagacct
     2881 atttaaatta tactttagac ctaaaaggga taaaagtttt aataaatgct ggagcaacag
     2941 ttgaagccat tgatcctatg cgttttctaa gtaatcgttc atctggaaaa atggggcttg
     3001 ctatagcacg cgctcttgct ttaagaggtg cagaagtaac tttactttat ggtaatcttc
     3061 aagttgagct tccttacaac atccaagata aagtattttg cgaaagtgca gatacaatgt
     3121 ttagagagac aatcaagcgt tatcccgaaa tggattggat aatttgttgt gcagcggtag
     3181 cagattataa acccaaagaa ataaataccc aaaaattacc aaaactctca caactcaatt
     3241 tggaactgat ttccactccg gatatcttat ttgagctagg taaattgaaa aaagagaatc
     3301 aacggttaat tggttttgct gcacaatccg ataatttaat aagaagtgcc ttagacaaat
     3361 attatagaaa aaatctggat cttatttgcg caaacgatat tcgttatgca ggcagggatg
     3421 aggatgaaat tattattcag ggtaagataa aaggttatcc tcccttaaaa gaagatccta
     3481 atataaactc tgtggtgatg aaaggagata aattttcttt ggcacataat ttgataaata
     3541 tcattaaaga gctatgagac agattttaat aagcggtgct aattctcagg taggaagcta
     3601 tctagcaaag gaatattata aagaaggatt ccagctggtt ttattgtatc ataaccgcaa
     3661 tgaacggatt gctgaacaat taggtaaaga aggcatattt tcctgttctg tagatctatg
     3721 tgacgctaca gccgttcagg cagctatttc agaatcggta aagcagttaa agcaaaattc
     3781 tgatatctta attcactgtg cggcagttcg cagttcggat gccttgccac tatttcaaac
     3841 agagccgttt caatttactt atgttttaaa aagtaatatt atatctgctt acaatgtatt
     3901 gcgctattct cttcctccaa tgcaggaaaa gcgatttgga agagtaataa tgtttggcag
     3961 tgatgttagc cgcaagggac ttaaaaatgg cagtgcttat gctgcctcca aagcagctat
     4021 agcaaatatg gttaaaagcg tagctctgga actggctaaa gataatattt taattaatac
     4081 tgtttcacct gctccgataa aaacagcttt ggaagaagat tatagtggag aatatctaca
     4141 atttaggcaa agatattttg ccgattattt aactaatgtg cctaccggta aatttgtaac
     4201 aaaagaggaa attaaaaaag tgatagactt gcttatcagt gaagacctgc aaaatctaac
     4261 tggagaagag atctttattg atgggggttc aaagtgaaag caaaattagg attaacctgt
     4321 ctgcttctag ttttgactct aaatttgata gcagcagctg ttccttttag ggatggagaa
     4381 aaattaacct ttactgtcaa atatggaata gttagtgcag gagaagcttc tttgcaagct
     4441 cgcagttctg tttatcaagg ttctccggtt tggtatcttt ctacaacagc taaaacttac
     4501 ccctttttcg ataaagttta caaagtaaga gatagagttg aatcctggtg ggataaagat
     4561 actttactac cttacaaatt tagcaaaaat cttcaggaag gtaattacag acagcatcga
     4621 gtgcatatat ataatcatca ggaaaagagt tctacatatc agaagtggag tttcaaaaaa
     4681 agtgtttttg agaacacaga aatggttatt cccgaaggga ctcaagatat attaagtgct
     4741 ttctatctga tgcgtactaa aaaattggaa gtgggaaaat ctgtaatggt caatattaca
     4801 gctgatggca gaaatatgcc tacagaagta gtagttcacc gcaaagagaa acaaaagact
     4861 atctttggca atgttgagtg tttagttatt gagccaaaac ttaaaggaga agcaattttt
     4921 aagcagagcg gtagaatttt aatttggctt accaatgatg attataaaat tcctgttcga
     4981 ctagaaagca agattactat aggtagcttt gtagctactt taaccagtgc ccaacaggtg
     5041 ccttttaaaa tccagttatg attcctgatc ttttagcaac aaatagttac gattattatt
     5101 tgcccaagga attaattgct caatatcctg ctgagcaaag aacggcatct cgattaatgc
     5161 atattaaaag aaacaccggg gaaattgctc atcgccaatt tactgatatt ttggaattga
     5221 ttaaggaagg agatctggta attctcaata gcagcaaggt ttttccagct cgtctttttg
     5281 ccgaaaagga aaatggtaca aaagtggaaa ttctgctttt atatcagctg aatgacaata
     5341 aatggaaatg tatggcttca ccggggaaaa gattaaaaca acctcaatac ttacacttct
     5401 ctgagaatct gtctggtttt atcagttctg gagatagaga aggattaaga gaaatcgatt
     5461 ttacctgcca aattaacttt tgggaagaaa ttcagagaat cgggcatatt cctcttccac
     5521 catatataga acgtaacgat actttttcag atagggagag atatcaaacc gtatatgcaa
     5581 aagaaattgg ctctattgct gctcctacag caggatttca ttttgataat gatattttag
     5641 atgctctccg agataaagga gtcctcttta gtgatgttat tcttcatatt gggattggta
     5701 ctttc
//
LOCUS       DAORHB010000083         5694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_14432_length_5694_cov_5.123781, whole genome shotgun sequence.
ACCESSION   DAORHB010000083 DAORHB010000000
VERSION     DAORHB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14432_length_5694_cov_5.123781"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKM80_03470"
     CDS             complement(<1..410)
                     /locus_tag="PKM80_03470"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425311.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNV92998.1"
                     /translation="MERFIIQGNRNLAGTIKVSGAKNAILPAMAACLLTSGKSVLHNI
                     PNLIDLKTMSHLLRVIGARVDNENGSMTIDARDVCYPEAPYELVSKMRASIYVLGPLL
                     TRLKEVRVSFPGGCAIGTRPVDLHLKAMESLGATV"
     gene            complement(413..1243)
                     /gene="prmC"
                     /locus_tag="PKM80_03475"
     CDS             complement(413..1243)
                     /gene="prmC"
                     /locus_tag="PKM80_03475"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNV92999.1"
                     /translation="MILAELIARAKNAAVANNIPQTDYWLVISYFLKLSRTEIFVFSQ
                     RVLTEQENITLTDAFRRLENGEPPQYITGEAYFYGLELRVNSFTLIPRPETERLVELT
                     LERLQGNEKILDIGTGSGAIAIALKQNLPKVEVYATDCSSSALEVAKANAEKYDVNIH
                     FFLTDCFPPCSEIYDVIISNPPYISKTDLATLDSRIRDKEPKLALYGGEDGLDFYRKL
                     LLQSYAYLTENGFLALEHGDNQKEAITNIAKKEGWTKILSRQDLTGRERYLFISGKKD
                     "
     gene            complement(1240..2157)
                     /locus_tag="PKM80_03480"
     CDS             complement(1240..2157)
                     /locus_tag="PKM80_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV93000.1"
                     /translation="MDTARILLVDDNISIIENLEMVLQANYFNVDTAYNGRDALHKIE
                     SDEYDLVICDIEMPGISGLDFLSTVRKNYNMELDVILMGSFLEYKDFSETIRLGAADF
                     IRKPIDNNQMLRSINTILKRKNNRNNFSELYNNLERVEFDFVLDPRNFSKFAVSELFN
                     NFLQQNFNLSHNLLNQILICVDEMVYNAFIHGTLALSMQERNLDHHKLQKIINKRLEQ
                     PDIASKRMHIHFSICHKTDTITVTVEDQGPGFDYEFWINKVKEDQKVNLEESGRGISM
                     LYYLSDKLEFQHGGRTVSISKKIPKTNKR"
     gene            complement(2160..2468)
                     /locus_tag="PKM80_03485"
     CDS             complement(2160..2468)
                     /locus_tag="PKM80_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV93001.1"
                     /translation="MNIKLSIEDKIAKMKIEGILNSENAYLLQEKLNDVLKSDATLLE
                     LDLLDCRNISSTGIGKILLFYKDFITKGGEIEVVRCSNSIYELFSMLKLNQLFTVNLG
                     "
     gene            complement(2519..4630)
                     /gene="fusA"
                     /locus_tag="PKM80_03490"
     CDS             complement(2519..4630)
                     /gene="fusA"
                     /locus_tag="PKM80_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV93002.1"
                     /translation="MNSDSDSPLSKIRNIGIMAHIDAGKTTTTERILFYSGYLHKMGE
                     VHNGNTFTDWMEQERERGITISSATVTCFWNEYKINIIDTPGHVDFTAEVERSLRILD
                     GAIGVFCGVGGVEPQSETVWHQADRYHVPRIAYVNKMDRIGADYYHVIQMIRERLTPY
                     AFPVNIPIGSEDEFEGVIDLITMQAYYFDPLTCGYEVKHSPVPETYLEQAKKMREELL
                     EKVSEFSDELMMLFLEGKDIPVDLLKSAIRRGTISNKLIPVFCGSSLKNKGIQLLLDG
                     ICDFLPNPMEIEDTQGFDPVTRNPITIASDPNGPLVALAFKVQIDKYVGRLVYIRVYS
                     GTLKKGASFIDQNNSKKERAARILQMMSNRKNDLDNLHAGDIGAIVGSKFLVTGDTIT
                     DGNLDILLSKMHFPDTVISIAIEPKTKSDQENLDIALHRLEEEDPTFRMHIDKDTGQT
                     LISGMGELHLEIIIDRLRREFGIAINQGNPQVAYKETITKEVEAAEVFQREMNGKGNY
                     ASVKFRLSPIALKDLPEDAKNVFINKVNEAQIPSEYWKPIEEAVFNALNDGPLISGNV
                     ERVRVELIGGDFNPVDSNELAYRIATGMAISTGLKGATPVLMEPIMLLTVISPDEFVG
                     DILNDINSKRGKIEVMRRDNEYRQEIVADVPLSELFGYATRIRSLSQGRAIYTLEFKK
                     YDICPVQVQNTVLKKIRGYVE"
     gene            5161..>5694
                     /gene="prs"
                     /locus_tag="PKM80_03495"
     CDS             5161..>5694
                     /gene="prs"
                     /locus_tag="PKM80_03495"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNV93003.1"
                     /translation="MFSKLKLITGNANRPLAEEVARHAGIPLVDIDLFKFSNDESFVK
                     INDNVRGADVFVIQPTCYPVNDNLIDLLIIIDALKRASAQRINCVIPYYAYARSDKKD
                     QPRVPITAKLIADLITVAGAHRVITVDLHADQIQGFFNIPVDHLYAIPTFARYFKSIM
                     PMDDIVVVSPDSGGANRA"
BASE COUNT         1762 a         1117 c          929 g         1886 t
ORIGIN      
        1 acagtggctc ccaagctttc cattgctttc aagtgcagat caacaggtct ggttccaatt
       61 gcacaaccac ctggaaaaga aactcttact tctttcaaac gagttaagag gggacccaga
      121 acatagatag aagcacgcat tttactaaca agttcataag gcgcttctgg ataacataca
      181 tcacgcgcat caatagtcat actgccattt tcgttatcaa ctctggctcc aataactctc
      241 aataaatgag acatcgtttt cagatctatc aaattcggaa tattatgtaa caccgatttc
      301 cccgaggtta ataaacaagc agccatagct ggtagaattg catttttagc accgcttact
      361 ttaatagtac cggccaaatt acgatttcct tggataataa aacgttccat ctttagtcct
      421 ttttacctga aataaacagg tatctttctc ttccggtcaa atcttgccgg gataggattt
      481 ttgtccagcc ctcttttttg gctatatttg tgatagcttc cttttgatta tcaccatgtt
      541 ccaaagccag aaaaccattt tcagttaaat aggcataact ttgcagaagt aattttcgat
      601 aaaaatccaa tccgtcttct cctccatata gagcaagttt tggctcttta tctctaattc
      661 tggaatccaa agttgccaaa tctgttttgc tgatatatgg tggattggaa attataacat
      721 catatatttc tgagcaggga ggaaagcaat cggtaagaaa aaaatgaata ttaacatcat
      781 atttttctgc attagcttta gccacttcca aagcggatga actacagtcc gtagcataca
      841 cttccacttt tggtaagttt tgttttagag caattgctat tgctccactt cctgttccaa
      901 tatccaggat tttttcattt ccctgtaaac gctctaaagt taattctacc aagcgttcag
      961 tttcgggacg agggattaaa gtaaaagaat ttactctcaa ttctaaaccg taaaaatatg
     1021 cttctccagt tatgtattga ggaggttcac cgttttccaa acgcctaaaa gcatccgtaa
     1081 gggttatatt ttcctgttca gttaatactc gctgactgaa aacaaaaatt tctgtcctgc
     1141 ttaattttaa aaaataggat attactaacc agtaatcagt ttgaggaata ttattagcta
     1201 cagcagcatt ttttgccctg gcgatcagct ctgctaagat catcttttat tagtttttgg
     1261 tattttttta ctgatcgaaa ccgtcctgcc tccgtgttga aactcaagtt tatcagaaag
     1321 gtaataaagc attgatattc ctctgccact ttcttccagg tttactttct ggtcttcctt
     1381 tactttatta atccaaaatt cataatcaaa gccaggtcct tgatcttcta cagttacagt
     1441 gatagtatct gttttatggc aaatggagaa atgaatatgc atccgttttg aggcaatatc
     1501 aggttgttca agacgtttat taataatttt ttgaagctta tggtgatcca aattgcgctc
     1561 ttgcatactt aatgccaaag ttccatgaat aaaagcattg taaaccattt catcgacgca
     1621 gatcaggatt tggtttagca gattatgaga gagattgaaa ttctgttgca aaaagttatt
     1681 gaataattct gatactgcga atttggagaa gtttcgcgga tcaagaacaa aatcgaattc
     1741 aaccctttcc aagttattat ataattcgct aaaattatta cgattattct tgcgcttcaa
     1801 aattgtattg atagaacgca gcatttggtt gttatctata ggtttacgaa tgaaatcagc
     1861 agcaccgagc cgaatagttt cactaaaatc tttatattcc aggaaactac ccatcaggat
     1921 aacatccaac tccatattat aattcttacg aactgtagat aaaaaatcta atccactaat
     1981 gcctggcatc tcgatatcac aaatcaccaa atcatattca tccgattcaa ttttatgtag
     2041 tgcatctctt ccattataag cggtatctac attaaagtaa ttagcctgca gtaccatctc
     2101 caaattttcg attatagaaa tgttatcatc cactaataga atgcgagctg tatccattgt
     2161 caacccaaat ttaccgtaaa taattgattc agtttaagca ttgaaaatag ctcataaata
     2221 ctgtttgagc atcggactac ttcaatttct cctccttttg taataaaatc tttgtaaaac
     2281 aacaaaattt tccctattcc ggtagagctt atattgcggc aatcaagtag atcaagctct
     2341 aatagagtag catcggactt tagcacatca tttagctttt cttgaagcag atatgcattt
     2401 tcgctattca agataccttc aattttcatc ttagcgattt tgtcttcgat ggatagctta
     2461 atattcatct tttcctcctt actttaacat ttttttattt cctttatttg tcaagcattt
     2521 attccacata cccgcgaatt ttcttcaaaa cagtattttg aacttgtaca ggacagatgt
     2581 catatttctt gaattctagg gtgtatatag ctcttccttg acttaaagaa cgaatccgag
     2641 tagcatatcc gaaaagttct gaaagaggaa catctgctac aatttcctgc ctatattcat
     2701 tatccctacg cataacttca attttgccac gcttggaatt aatatcgttt agaatatcgc
     2761 ccacgaattc atcaggagag ataactgtca gcaacataat gggttccatt aaaacaggtg
     2821 tggccccttt taaacctgta cttatagcca tacctgttgc aattcgatac gcaagttcat
     2881 ttgaatctac tggattaaaa tccccaccaa taagttccac gcgaactctt tccacatttc
     2941 cactaattaa gggtccatca tttagtgcgt tgaaaactgc ttcttctata ggcttccaat
     3001 attcagaggg aatttgggct tcatttactt tattaataaa gacattctta gcatcttcag
     3061 gcaagtcttt taatgcgatt gggcttaagc gaaacttcac agaagcataa tttccttttc
     3121 cattcatttc gcgctggaag acctctgctg cctcaacttc tttggtaata gtttccttat
     3181 aggcaacttg agggttaccc tgatttatag ctattccgaa ttcccgtctt aatcgatcta
     3241 ttataatttc cagatgcaat tctcccattc cggaaatcaa tgtctgtcct gtatctttat
     3301 ctatatgcat cctaaaagtg gggtcttctt cttccaatct atgcagtgct atatccagat
     3361 tttcctgatc tgatttggtt tttggttcaa tagctataga gataacagta tccggaaagt
     3421 gcattttaga gagcaaaata tccaggttac catcggtaat tgtatcccca gtaactaaaa
     3481 attttgaacc gacaatagct ccaatatcac ctgcatgcaa attatccaaa tcgttcttgc
     3541 gattactcat catttgtaaa atgcgtgctg ctctttcttt tttgctatta ttctggtcaa
     3601 tgaaggaagc accttttttt agagttccgg aataaacacg aatataaaca aggcgaccaa
     3661 catatttatc gatctgaacc ttaaaggcca aagctaccag aggaccatta ggatcggaag
     3721 caattgttat cggattacgg gttacggggt caaaaccttg tgtatcttca atctccatag
     3781 gatttggcaa aaaatcacaa attccgtcta aaagcagctg tataccttta tttttaagcg
     3841 aacttccaca aaaaaccgga atcaatttat tgctaattgt tcctcggcga attgcacttt
     3901 ttaataaatc tacagggata tcttttcctt ccagaaatag catcataagc tcatcactga
     3961 attcactaac tttttctaat agttcttccc gcattttttt tgcttgttcc aaataagttt
     4021 ctggaacagg agaatgctta acttcatagc cacaggttaa aggatcaaaa taatatgcct
     4081 gcattgtgat caaatcgatt acaccttcaa actcatcttc gctgcctata ggaatattta
     4141 ccggaaaagc ataaggcgta agccgctctc taatcatttg aataacatga taataatctg
     4201 ctccaatccg gtccatttta ttcacataag caatccgggg aacatgatat cgatctgctt
     4261 gatgccaaac tgtttcggat tgaggttcca cacctccaac tccacaaaaa actcctattg
     4321 ctccatctaa aatacgaagg gaacgttcta cttctgctgt aaaatctaca tgaccaggag
     4381 tgtctataat atttatttta tattcattcc agaaacaagt aacagtagct gatgatatag
     4441 ttattcctct ttcacgttcc tgttccatcc aatccgtaaa ggtattgcca ttatgcactt
     4501 ctcccatttt atgcaaataa ccactataaa acaatatcct ttccgtggtt gtcgtttttc
     4561 cggcatcaat atgagccata atgcctatat tgcgtatttt tgatagtgga ctatccgaat
     4621 cgctgttcat acaaaattcc tttttcatgt tttgtgaacc attaaaacaa ggttagtttg
     4681 tttgtctatg aaaaaagtag cttgtggcta tctaaaatgc aaataattga agaaattaac
     4741 tgtactagca tcaatatata tgagcattag gaaagcaatg gaccctgccg aaataactga
     4801 tatagtttgc ataacgagga tgcggttatt aaaaaaatta tctggttctt ttaaagccag
     4861 aatgataaat agctcttcca taaagaaata atattataat caaccaggta aggtgatttc
     4921 tattgattgc cctaaaattg atatatagta cattgattat tcaaaatatt agcggtatgc
     4981 gtgctaaaaa gaaataagct ttgacaaaaa gcatagctat aaaattgtgg attaacatag
     5041 tttattttat ctgggaacag atatataaaa gatagatatt tagtaacttg catagctgaa
     5101 aaggcaatgc agaaagagga aaattaactg atcagggaaa acaaaaaaga agggaagcaa
     5161 gtgttttcaa aactaaagct tatcacagga aatgctaatc gtcctttagc agaggaagta
     5221 gctcgtcatg caggtattcc tctggtagat atagatttgt tcaaattttc taatgacgaa
     5281 tcgtttgtta aaataaacga taatgtccgc ggtgctgatg tttttgtaat tcaaccgact
     5341 tgttatccgg ttaacgataa tttaatagat ctactgatca ttattgatgc tttaaaaaga
     5401 gcttcagcac agcgaattaa ttgtgtgata ccttactatg cgtatgctcg ttctgataaa
     5461 aaagaccaac cccgagttcc aattacagca aagttgattg ctgatttgat tactgtggca
     5521 ggtgcgcata gagtaattac tgtagattta catgcagacc agattcaagg tttcttcaat
     5581 attcctgtag atcatcttta tgctattcct acttttgccc gctattttaa aagcattatg
     5641 ccgatggatg atatagttgt agtttctccc gattcgggtg gagcaaatag agca
//
LOCUS       DAORHB010000084         5681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_14480_length_5681_cov_5.310700, whole genome shotgun sequence.
ACCESSION   DAORHB010000084 DAORHB010000000
VERSION     DAORHB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14480_length_5681_cov_5.310700"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PKM80_03500"
     CDS             complement(<1..872)
                     /locus_tag="PKM80_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNV93004.1"
                     /translation="MEKYIDDFCNYLALEGKSEKTISAYKLDLEQFYTYIQRFFENGI
                     VTLPEITVLNIRDFLRYLNEKPDCNRSLARKSAALNSFFKFCKKSGFISNNPMDKIKR
                     PKYEVPLPKCFTEEEVRTLLSIPDTSNRYGVRNKAILETLYSSGLRLSELAGIRLQDI
                     DLKRGLVRVTGKGNKQRIVPLGSYAREAIYNYLKIRPKFAQENSPDLLFLTKSGKAFD
                     TKQLDIILKRYFELVAKAKGYSPHSLRHSFATHLLSRGADLRAIQELLGHSLLSTTEV
                     YTHISMEDIKEAYK"
     gene            943..1698
                     /locus_tag="PKM80_03505"
     CDS             943..1698
                     /locus_tag="PKM80_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNV93005.1"
                     /translation="MNQYIDNSAKIGKNVTFGYNVVIMEGVQIGDDCLIGHNVVIHPD
                     TKIGNSCRIDDGTIIGKKPLSSPRSIFSVSTNLKAAEIGDFCQIGSNAVIYCQCEIGN
                     HNLIADLATIRENVSIGDLNIIGRNVAIENYVKIGNRNKFETNCYITAYSEIGNYCFV
                     APCVATSNDNYMGRDIERYKHFRGITMIDGSRIGLNATILPGKTINSDGTVAAGAVVT
                     KDVPVETVVAGNPAKPMRTVPETQLMKHNLDIQ"
     gene            1695..2417
                     /locus_tag="PKM80_03510"
     CDS             1695..2417
                     /locus_tag="PKM80_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNV93006.1"
                     /translation="MKTLIIIPTYNEIENIERLLEQVLSKSDSIEVLVIDDNSPDGTA
                     LRVKFMQSTNPRIHLLQRPGKMGLGSAYVTGFKYALEQGYDFIMEMDADFSHNPDDIP
                     RLLEAAQKYDLVIGSRYCEGVNIIHWPFKRLLISYFASKYVRVITGMPIKDPTGGYKC
                     FRRKVLESIDLDKILSDGYAFQIEMNFRAWVKGFHLKEIPIIFTERINGVSKMSRKIV
                     WEAAWMVWRLQIMKVLGLINNK"
     gene            2424..2885
                     /locus_tag="PKM80_03515"
     CDS             2424..2885
                     /locus_tag="PKM80_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93007.1"
                     /translation="MMKRFTLFSILLLLCLTLSAITIPRVYVQKLTLENDKNPLITRE
                     QDKSANEYILKAWINTRPDEIVSTETHPIHTIAVKQVGDGNKFPFMVITSLQLGNFKS
                     QWQAGEVIYMEITHIKTGQKKTWTQPIPEGTNLIKMLDKPVVIPPYTKKKK"
     gene            2889..3905
                     /gene="ruvB"
                     /locus_tag="PKM80_03520"
     CDS             2889..3905
                     /gene="ruvB"
                     /locus_tag="PKM80_03520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNV93008.1"
                     /translation="MLERINDPDQLIEDIELDRALRPRSLKEFIGQTHIKELLDISIK
                     AARLRGESLDHILLYGPPGLGKTTLAGIIAYELGVNITTSSGPVIEKPSDLAGILTNL
                     QGHSVLFIDEIHRLSHVIEEYIYPAMEDFEMEIILDSGPNSRTLKIPLEQFTLIGATT
                     RAGLLTPPLRDRFGIVLRLDYYDQESIAHIIRRSAKLLNIPADNDGINEIARRSRGTP
                     RIANRLLRRVRDYAQIKGNGIITLNIALAALQMLQVDNAGLDEMDKRILTTIIENYNG
                     GPVGIKTIATAVGEDSGTIEEIFEPYLVQQGFLERSPQGRKVTFKAYRHLGLTPLTEQ
                     TEIF"
     gene            3915..5342
                     /locus_tag="PKM80_03525"
     CDS             3915..5342
                     /locus_tag="PKM80_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNV93009.1"
                     /translation="MHAKIVKSGVRWTLATTILRRGISLILFIFLAKWLTQTDFGIYR
                     SFSAILALLTYLTHFGLDYLFLISKSKERINFLALLQTGLFVSAVITILLIFFGKTIG
                     TYYNSAELGRILTYGGGLIFVESLRRIARVYAQKRFQFKELAIAETLNVFIYSLLCLG
                     IIYFWRKAWVYILLFYIGNLGELIYLCIKLPPLPATLQKQVFSFKWLKHSCAYFKRNF
                     QFLGIVSTINLLQSYSINAPVLFLGTMVEPALMGVYFFASQLIAIPIGMLNTSLSQVF
                     FPVLAQSETAASVSGIRHYTAIVLKIGIPALIFYALILQYLVPLIWGDKWLSALPLVL
                     YLVLFYGTSMLHNPISGIPYICRKPQWELLWNIVTLGLRIIALFLGMKVSFAFAILLF
                     CIVSAFMHLAFYYLSLTLLKANLWQITIDLISNLPVLILIAFGCLYLWKFSVIFPLLT
                     FVGYGLYLFLAEKDTLKEVLSLAIK"
BASE COUNT         1751 a         1003 c         1083 g         1844 t
ORIGIN      
        1 tttttatatg cttccttgat atcttccatt gatatatggg tatatacttc tgttgtagag
       61 agaagtgaat gccccaacag ttcttgaata gctcgtaaat cggcacctct ggaaagtaaa
      121 tgtgtggcga aactatgtct taaagaatgc ggggaatagc ccttagcctt agctaccagt
      181 tcaaaatatc gttttagaat gatatccaac tgctttgtat cgaacgcttt gccgctttta
      241 gttaaaaata gtaaatcggg gctattttcc tgggcgaact ttgggcgaat ctttaaatag
      301 ttatatatgg cttctctggc ataggaaccc agggggacaa ttcgctgttt attacctttt
      361 cctgtaaccc gaaccaggcc tctttttaaa tctatatcct gtaaacggat gccagcaagt
      421 tcagaaagac gcagacctga agaatataag gtttctaaaa ttgccttatt tctaacacca
      481 taccgattgc tggtatctgg aattgaaagc agagttctta cttcttcttc ggtaaagcat
      541 ttgggcagag ggacttcata tttggggcgt ttgattttgt ccatcgggtt atttgaaata
      601 aagccgcttt ttttgcaaaa cttgaaaaaa gaatttagag ctgcactttt acgcgctaga
      661 gaacgattgc aatctggctt ttcattcaaa tagcgcaaaa aatcgcgaat attcagaaca
      721 gttatttccg gcaaagtaac tattccattt tcaaaaaaac gctgaatata ggtgtaaaac
      781 tgctctaaat ccaatttata ggcactaatc gtcttttcgg atttaccttc taaagccaga
      841 tagttgcaaa aatcatctat atacttttcc ataaggccat tttttacttg ccttaaaact
      901 tgtcgagtaa aaaatggtat taaagtaaaa aggggaggat ttatgaacca atatatagat
      961 aattccgcca aaattgggaa aaatgtaact ttcggttata atgtagttat aatggaagga
     1021 gttcaaattg gagatgattg cttaatagga cataatgttg ttattcatcc ggatacaaaa
     1081 atcggtaatt cctgtagaat tgatgatgga acgattattg ggaaaaaacc actctcttct
     1141 ccacgcagta tttttagtgt gtccaccaat cttaaagctg ccgaaattgg agatttttgc
     1201 caaattggca gcaatgctgt tatctattgt caatgtgaaa taggtaacca taatttaatt
     1261 gctgatctgg ctactatccg tgaaaatgtt agtataggtg atttgaatat tatcggcaga
     1321 aatgtagcta tcgaaaacta tgtaaaaatc ggaaaccgaa ataagttcga aactaattgc
     1381 tatatcactg cctattctga aattggaaat tattgttttg tagctccttg cgtggcaaca
     1441 agtaacgata attatatggg gcgcgatata gaacgctata aacactttag aggaattaca
     1501 atgatcgatg gttcaaggat tggtcttaat gctacaatcc ttcccggaaa aacaataaac
     1561 agcgatggaa ctgtagctgc tggagcagtt gttaccaaag atgttcctgt ggaaactgtt
     1621 gttgctggaa atccagcaaa acctatgcgc acagtacctg aaacccaatt aatgaaacat
     1681 aatctggata tacaatgaaa acactaataa ttatccctac ttacaacgaa attgaaaata
     1741 ttgaacgcct cctggaacag gtgctttcta aaagtgactc aattgaagta ttagtgatag
     1801 atgataattc tcctgatggc acagctttaa gagtgaaatt tatgcaaagc acaaatccgc
     1861 ggatacattt acttcagcgt ccagggaaaa tgggtctcgg ttctgcttat gtaaccggtt
     1921 tcaaatatgc attggaacaa ggttacgatt ttataatgga aatggatgct gatttttctc
     1981 ataatccaga tgatataccg cgcttgttgg aagcagccca aaaatatgat ttagttattg
     2041 gtagtcgcta ttgcgaaggg gtaaatatta ttcactggcc tttcaaacga ttattaatta
     2101 gctacttcgc cagtaaatat gtgcgcgtaa taactggaat gccaattaaa gatcctaccg
     2161 gagggtataa gtgttttcgg cgtaaagttc tggaaagtat agatttggac aaaatcctat
     2221 ctgacggcta tgccttccag atagaaatga atttccgagc ttgggtgaag ggtttccatc
     2281 taaaagaaat accaattatc tttaccgaac gcatcaacgg cgtatcaaaa atgagccgaa
     2341 aaatcgtttg ggaagctgcc tggatggttt ggcgtttaca aattatgaaa gttctgggtc
     2401 ttataaataa taaatagggt gttatgatga agagatttac tctgttcagt atattgctac
     2461 tgctttgttt aaccctttct gcaattacta tcccccgagt ttatgtgcaa aaactaactc
     2521 tcgaaaatga taaaaaccct ttgataactc gtgaacaaga taaaagtgct aatgaatata
     2581 tccttaaggc ctggattaat acccgcccgg atgaaattgt cagtactgaa actcatccga
     2641 ttcatactat tgctgttaaa caagttggag atggaaataa gtttcctttt atggtgatta
     2701 ccagtttgca acttggtaat tttaaaagcc aatggcaagc cggagaagtt atctatatgg
     2761 aaataacaca tataaaaaca ggacaaaaaa agacctggac ccagcccatc cccgaaggca
     2821 caaacttaat taaaatgctg gataaacctg ttgttatccc accctatact aaaaagaaaa
     2881 aataaactat gctggaaaga ataaacgacc ccgatcaatt gattgaagat atagaactgg
     2941 atagagcttt acgtccgcgc tctttaaaag aattcatcgg acaaacacat attaaagaac
     3001 ttttagatat tagtatcaaa gctgccagac tacgcggtga atcccttgat catattttat
     3061 tatatggacc tcccggttta ggaaaaacta ccctggctgg cattattgct tatgaattag
     3121 gggtaaatat cactacctcc agcggaccgg ttattgaaaa accttctgat ttggctggta
     3181 ttttaactaa tttacaaggg cattcagtcc tttttataga tgaaattcat cgcctgtccc
     3241 atgtgattga agaatatatc tatcctgcta tggaagattt tgagatggaa ataattctgg
     3301 atagcggtcc caattcccgc actctaaaaa taccattgga acaatttact ttaattggag
     3361 ccactaccag agctggttta ttaactcccc ctcttcgaga tagatttgga attgtgcttc
     3421 gtttggatta ttacgatcag gaatccatag cacatattat tcgtcgtagt gcaaaactat
     3481 tgaatattcc agctgataat gatggcatta atgaaattgc cagacgcagt aggggcactc
     3541 ccagaattgc caatcgttta cttagacgtg tgcgtgatta tgctcaaata aaaggcaacg
     3601 gaattattac tctaaatatt gctttggctg ctttacaaat gttgcaagtg gataatgccg
     3661 gtttggatga aatggataaa cgcatcttaa ctactattat cgaaaactat aatggaggcc
     3721 ctgtaggtat taaaacaatt gctactgccg ttggagaaga ttccggaact atcgaagaaa
     3781 tctttgaacc ctatttagtg caacagggct ttctggaacg cagccctcaa ggtagaaaag
     3841 ttacttttaa ggcataccgc catttaggtt taactcccct tactgaacag acagagattt
     3901 tctaaaagag aaaaatgcac gcaaaaatag ttaaaagtgg agttcgttgg actcttgcaa
     3961 ccactatttt acgcagagga atcagtttga ttcttttcat ctttctggct aaatggttaa
     4021 cgcaaactga tttcggtatc tatcgtagct tttcagcaat tttggcgttg cttacttatc
     4081 taactcattt tgggcttgat tatctgttcc taatttccaa gagcaaagaa cgcataaact
     4141 tcctggctct tctgcaaaca ggactttttg tttcagctgt tattaccatt ctgctaatct
     4201 tttttggaaa aactatcgga acttattata acagtgctga acttggcaga attctcactt
     4261 atggcggggg cttaattttt gtggaatccc taagaaggat agctcgcgtt tatgctcaaa
     4321 aaagatttca gttcaaagag ttggcaatag cggaaacact taatgttttc atctattctt
     4381 tgctctgctt gggtattatc tatttttggc gaaaggcctg ggtttatatc cttttatttt
     4441 atatcggcaa tttaggtgaa cttatttatc tatgcatcaa gctacctcct ttaccagcaa
     4501 ctttacagaa acaggttttt agttttaagt ggctaaaaca ctcttgtgcc tattttaagc
     4561 gtaactttca atttttggga atagtaagta cgataaatct gttacagtct tattccatca
     4621 atgctcctgt ccttttttta ggaactatgg tagaaccagc tttgatggga gtttattttt
     4681 ttgcatcgca actaattgca attcctatag ggatgcttaa tacttctctt tcgcaggtat
     4741 tttttccagt tctagctcag agtgagactg cagcaagtgt ttctggcata aggcattaca
     4801 ctgctattgt tctgaaaata gggatacctg cactaatttt ttatgcgctt attttgcaat
     4861 atctggttcc tcttatatgg ggcgataaat ggctctcggc attgcctttg gttctctatt
     4921 tagtcctttt ttatggaaca agtatgttgc ataatccaat tagtggcatt ccctatattt
     4981 gtagaaaacc tcaatgggaa ttgctttgga atattgttac tttaggtttg cgtattatag
     5041 ctctgttttt gggaatgaaa gtttctttcg cttttgccat tttattattt tgcattgtga
     5101 gtgcatttat gcatctggct ttttattatt tgtctttaac tctgttaaaa gcaaatttgt
     5161 ggcaaataac aattgacctt atatctaatt tgccggtatt gattttaatt gcctttggct
     5221 gcctttatct atggaagttt tctgtgatat ttccgctttt aacttttgtg ggttatggat
     5281 tgtatctatt tttggcggaa aaagatactt taaaagaagt gctttctctg gctataaaat
     5341 agccctaaga ttatgcaaat tataatttta gaaacaagga ttataggata tcaagattta
     5401 ttataaacac aaaatatgac ttctggcttg ctgatggggc gtaaagaaaa caataccccc
     5461 ctttttaata agttaaatta ctaaggaaaa agtaatattc tacttctgca attcatcagg
     5521 aattttcctc tgagtaaaaa tccgcagaag aaagccacat agtttatagc catattcagt
     5581 aactgaaccg ccgaataaaa tcattctaaa aacaggttag cacaaggtct gttgacaaac
     5641 ttcggtttat ttgcccagca aaaaatggta agctcttttt t
//
LOCUS       DAORHB010000085         5574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_14889_length_5574_cov_4.907592, whole genome shotgun sequence.
ACCESSION   DAORHB010000085 DAORHB010000000
VERSION     DAORHB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14889_length_5574_cov_4.907592"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKM80_03530"
     CDS             <1..416
                     /locus_tag="PKM80_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNV93010.1"
                     /translation="VEEGIVPGGGVTLIHAAKALKQMKGLSFEEKMAVDILSKALEKP
                     AYQIAANAGEEGAVVVEKLKGFRDIHMGFNAANGEYEDLFKAGIIDPAKVVRSAIQNA
                     ASIAGLMLTTECTITDIKEPEPAVPMPNPGMGDMY"
     gene            1307..3832
                     /locus_tag="PKM80_03535"
     CDS             1307..3832
                     /locus_tag="PKM80_03535"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNV93011.1"
                     /translation="MKEGLIQKNDYHSIFFSDRAEECGSEIKALFLNHLEYSLIKDTT
                     TVRNWDIYYALALSLRDLLIERWLRTQYEYRKQDVKKVYYLSMEFLLGRMLTNSLINL
                     EVYNEVYDMLKQMGYRLEDIIELEPDMGLGNGGLGRLAACFMDSLATQAFPAYGYGIR
                     YDFGIFKQQIVQGYQVEEPDHWRKNGCPWEINRPELTYRVRFGGKVITQTLPDGRIEY
                     HWVDTEDVMAVAWDIPVPGYEVDNVNNLRLWQATSSDEFDLEYFNSGDYVKAVEKKTI
                     SENISKVLYPNDNVHLGKVLRLQQEYFFVSATLQDIFSHWKRDHNSFTDLPEKIAIQL
                     NDTHPALAIPEMMRILIDEDRLSFDSAWEITKRCFSFTNHTILPEALEKWSISLFEEL
                     LPRHLLLIYQINSVVMEEVIRLYPGDIVKMRNVSIIEETNGKAIRMAQLAIHSAHKVN
                     GVAKLHTKILCERIFPDLAEMYPDKFHNITNGITPRLWLHTCNPQLASLITEYIGNGW
                     IRNLEEISKIEQYIDNYDFRTSFAEVKQINKNHLSKYIYRETGIRVQDNSLFYTQIKR
                     LHEYKRQLLNVLGAIARYFQIKDNPQGDFVPHTVIFAGKAAPGYYLAKRLIKLINNIG
                     EVVNKDPDVKDRLKVVFLPNYSVSLAEKIIPATDLSIQISTAGYEASGTGNMKFALNG
                     ALTLGTLDGANIEMSEEIGIENMFIFGLNAEQVRHLQQSGYNPYSYYENDPELKRVVD
                     SLIDGSFEEGEKGLFLPIWKSLMEEGDLYLNLVDFRAFMDAFDRVDTLYQNREQWITK
                     AIINVSKMGRFSSDRAIMEYANQIWNIQPLPLVLNNGNNNSSV"
     gene            3829..5445
                     /locus_tag="PKM80_03540"
     CDS             3829..5445
                     /locus_tag="PKM80_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNV93012.1"
                     /translation="MNRIKTCLLFLCGCLYLFPLTAAIVINEVCYDPVGSDDGFEWIE
                     LFNNGNSSEQLEGAKIVSGGASFTLQYTIPFFELRPGRYLLIGGSSVPNAQLIYNFSF
                     QNGGSETDGICYISPDGIYTDTVLYDSPNTNQLIDDTNYPGIYFAPDVIAGNSLARII
                     DGLDTNNCAIDFIAEANPTPGLPNRRHCDYAFGQYNFNYDYGFAEIDLWLKNLSSITP
                     LISAELSVMQNDDLLAQQIISPIPALDSLWVNLAFYCNNYPLIIKLNLTDDPDSTNNI
                     LLIPINPDMEQGIYINEYLANPETNNQEWIELYCGIKQRGDYYIEDSSNSKIRFSLPE
                     NSGYYVICQYPEELLIRYPDCPIGSVILAESWTFLNNSGDLLVLKNESTTLDSISYTG
                     EEILKGISRERVLSDSTVFWQNCYNQIGGTPGLPNSILPQTELPDLGKVTLSGSPCNA
                     KAGEKITLAYNFPAETNRISCRIFDLSGRKICTIADYSLVNASGFVHWNGCKQDGTFA
                     PRGLYIILWESQNASGGKIMRKQFTAVLKG"
BASE COUNT         1776 a          904 c         1100 g         1794 t
ORIGIN      
        1 cagttgaaga aggaattgtt cccggtggtg gagttacttt aattcatgct gcaaaagctc
       61 ttaagcaaat gaaagggctc tcttttgaag agaaaatggc agtggatatt ctttctaaag
      121 cgttagaaaa acccgcatat caaattgctg ctaatgctgg agaagaaggt gccgtggtag
      181 ttgaaaaact aaaaggtttc cgcgatatcc atatgggatt taatgctgca aacggtgaat
      241 atgaagacct cttcaaagcc ggaattattg atcctgccaa ggttgtgcgt tctgctatcc
      301 aaaatgcagc ttccatagct ggtttgatgt tgactactga atgcacaatt accgatatca
      361 aagaaccaga acctgcagtt ccaatgccca atccaggtat gggtgatatg tattaatttc
      421 acaataaatc atataacaag aatgggcctc tcaatccagg agaggctctt ttatttaata
      481 agcaattatc aataaaaaaa agactcctgc agattgcgct gcaaattaaa acattagatc
      541 tgaagtgaac aagatttggc tagtatagtt aaaaattatc ccctctatag tttttattcc
      601 tataagacac atataagcat caattacaat tggaaagatt aaagtaacaa tataaaaata
      661 tcacgataat tcccctcttg aaactgcatt ataccaactg taacaatcaa ttttgtaata
      721 aaatcccaat aattttgtct cctattatcc ttgctcgagg ccattaatct gtcgtttatc
      781 atcccttaat caagcgttaa taattaacgc ttgattaagg ttagataaac acttgattaa
      841 cgctttccat taagagcgag tagttgaagt cgaagccgat agacagttta tccaaaagta
      901 ataaatacct gatgacagat agatatatta ggaaagtact taatatcaat ccataatttt
      961 aaagttcggg gaaaatgagc taacctgttc ttgcttggca aataaactga agttaatcag
     1021 cagactttga gcttgcttgt ttttaggata attttattca acgattcagt tgctgaaaat
     1081 gtctataaac tatgtggtta cattattggg attttttggt tagcgggaaa tttctgatga
     1141 attgtagaaa cagaatataa cttttttctt aggaactgaa cttaataaaa aagggtggta
     1201 cttttttcct ttactcggca aagagattct gcactttttt cttgacaaca aatcttaatg
     1261 cagcatttgg attaaaatct atacatataa agctagggaa caactaatga aagaaggttt
     1321 aatacaaaaa aatgactatc attccatatt cttttcagat cgagcagagg aatgtggaag
     1381 tgagataaag gccttatttc taaatcatct ggaatattca ttaataaaag atacaaccac
     1441 tgttagaaat tgggatatat attatgcttt ggctctcagt ttacgcgatc tattaattga
     1501 gagatggtta cgcactcaat atgaatatcg aaagcaggat gtgaagaaag tctattatct
     1561 ttcaatggaa ttcttgctgg ggcgaatgct aaccaacagt ttaataaatc tggaagtgta
     1621 caacgaagtt tacgatatgt taaagcagat gggttaccgc ttggaagata taattgaatt
     1681 ggaaccggac atgggtttag gaaatggagg tttaggacgt ttagctgcct gttttatgga
     1741 ttctttagcc acccaagctt ttcctgctta tggttatgga attcgttatg attttggcat
     1801 attcaaacag caaattgttc agggttatca agttgaagaa ccagaccact ggcgaaaaaa
     1861 tggttgccca tgggaaataa atcgtccgga actcacatac cgagttcgtt ttggaggcaa
     1921 agttattacg cagacccttc ctgatggcag aattgaatat cattgggttg atacagaaga
     1981 tgttatggct gttgcttggg atattcctgt tcctggttat gaagtagata atgtaaataa
     2041 tttacgtctt tggcaagcaa ccagcagtga tgaatttgat ttggaatatt ttaatagtgg
     2101 ggattatgta aaagcggtgg agaagaaaac catttccgag aacatcagca aagtgctcta
     2161 tcccaatgat aatgttcatt taggtaaggt gttgcgtttg cagcaggagt atttttttgt
     2221 ttctgctacg ctccaggata ttttttcaca ttggaaaagg gatcataata gctttacaga
     2281 ccttccagaa aagattgcta ttcagttaaa tgacactcat ccagctcttg ccattcctga
     2341 aatgatgcgt attttgattg atgaggatcg tctcagtttt gattctgcct gggagattac
     2401 aaagcgttgt ttttctttta caaaccacac aattttacct gaagctttag aaaaatggag
     2461 catttctctt tttgaggaat tactgccgcg tcatctatta cttatttatc aaataaacag
     2521 tgtagtaatg gaagaagtta ttcgcttata tccaggtgat attgttaaaa tgcgtaatgt
     2581 ctctataatt gaagaaacta atggaaaagc aattaggatg gctcagttag caattcatag
     2641 tgcacacaaa gtgaatgggg tagccaaatt acataccaaa attctatgtg agagaatttt
     2701 ccccgatctt gctgaaatgt atcctgataa atttcataat attacgaatg gtatcactcc
     2761 tcggctttgg ctgcatactt gtaatcctca attagccagt ttaattacag aatatatagg
     2821 aaacggctgg attaggaatt tggaagaaat tagtaaaatt gaacagtata tagataatta
     2881 cgactttcgt acttcctttg ctgaagtgaa gcagatcaac aaaaatcact taagtaaata
     2941 tatttaccga gaaacaggta tcagagtgca ggataattct ctcttttata ctcagataaa
     3001 acgattgcat gagtataaac gtcaattatt gaatgtgtta ggagcgattg ctcgttattt
     3061 tcagataaaa gataatccgc aaggtgattt tgttccgcat acagttattt ttgccggaaa
     3121 agcggctcct ggatattatt tagctaaacg actgataaaa ttgataaata acattgggga
     3181 agtggtgaat aaagatcctg atgttaaaga ccgtttgaaa gtagtcttct tgccaaatta
     3241 ttctgtttct ctggcagaaa agattattcc ggcaacagat ctttccattc aaatttctac
     3301 tgcaggttac gaagccagcg gaacgggaaa tatgaaattt gctttgaacg gagctttaac
     3361 cctgggaact ttggatggag ccaatatcga aatgtctgaa gaaatcggaa ttgaaaatat
     3421 gtttattttt ggcttgaatg cagagcaggt aagacatcta caacaaagcg gttataaccc
     3481 ctatagttat tatgaaaatg atcctgaact gaagagagta gttgattcct tgatagatgg
     3541 tagctttgag gaaggcgaaa aaggtctctt tctgcctatc tggaaatcac ttatggaaga
     3601 aggtgactta tatctaaact tagttgattt tcgtgctttt atggatgcat ttgatagagt
     3661 tgatacactt tatcaaaacc gcgaacagtg gataacaaaa gctataataa atgtatctaa
     3721 aatggggaga ttctccagcg atagagcgat tatggaatat gctaatcaaa tttggaacat
     3781 tcaaccctta cccttggtat tgaacaacgg gaacaacaat tcttctgtat gaacaggata
     3841 aaaacctgtc tcctctttct ttgtggctgt ctttatcttt ttcccttgac tgctgccatt
     3901 gttataaatg aggtttgtta cgatccagtt ggttctgatg atggttttga atggatagaa
     3961 ctttttaata acggcaatag ttctgaacaa ctggaagggg caaaaattgt ctctggagga
     4021 gcttctttta cccttcaata cactattcca ttttttgaat taagaccagg tcgttattta
     4081 ttgatagggg gttcctcggt tcctaatgct caacttatct ataattttag ttttcaaaat
     4141 ggtggtagtg aaacagatgg tatctgttat attagtccag atggaattta tactgatact
     4201 gtgctttacg attctcctaa tacaaatcaa ctgatagatg atactaatta tcccggaatt
     4261 tattttgcac cagatgttat agcaggtaat tctctggcaa gaattattga cggtttggat
     4321 actaataatt gtgcaataga tttcattgca gaagcaaatc ctactcccgg cttgccaaac
     4381 cggcgccatt gcgattatgc ttttggccaa tataatttta attatgatta tggctttgcc
     4441 gaaatagatc tgtggttgaa aaatcttagt tccatcactc ctctaatatc agcagaatta
     4501 tctgttatgc aaaatgatga tttactggct cagcagatta tttctcccat tcctgcatta
     4561 gattcattgt gggttaattt agctttttac tgtaacaatt atcctctgat tataaagcta
     4621 aatttaacag acgatccgga ttctaccaat aatatattat tgattcctat aaatccggat
     4681 atggaacagg gaatttatat caacgaatat ttggctaatc ccgaaaccaa taaccaggaa
     4741 tggattgagc tctactgtgg aattaaacag cgaggggatt attatattga agatagttcc
     4801 aattccaaga tccgtttttc tttgcccgaa aacagtggct attatgttat ctgccaatat
     4861 ccagaggaat tacttattcg ctatccggat tgtccaatag gttctgttat tttagctgag
     4921 agttggacat ttctaaataa ttctggagat cttttggttt tgaaaaatga atcgacaact
     4981 ttggattcta tatcttacac tggagaggaa atattaaaag gaatatcccg tgaaagagtt
     5041 ttgagcgatt ccactgtttt ctggcaaaat tgttataacc aaataggagg gactcctggt
     5101 ttaccaaata gtattttacc ccaaacagaa cttcctgatt tagggaaagt tacgctatct
     5161 ggaagtccct gtaatgctaa agctggcgaa aaaatcaccc ttgcttacaa ttttcctgct
     5221 gaaactaatc gtatttcctg ccgtattttt gatttgagtg gccgtaaaat ttgtacaatt
     5281 gcagattatt ctttagtaaa tgcttcagga tttgttcatt ggaatggttg caaacaggat
     5341 ggcacttttg cccctcgcgg actttatatt attttatggg aatcgcaaaa tgcgagtggg
     5401 gggaaaataa tgcgcaaaca atttactgct gttctgaaag gataattgct attttcttcg
     5461 gttgagaagg attagagggt ttagaaaatt caaaactctc agagcgacac aatgatagtg
     5521 atgggtgcgt aaagcactcg aaacgaggat tttaggactg aattttagaa acaa
//
LOCUS       DAORHB010000086         5564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_14926_length_5564_cov_4.483028, whole genome shotgun sequence.
ACCESSION   DAORHB010000086 DAORHB010000000
VERSION     DAORHB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14926_length_5564_cov_4.483028"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..161
                     /locus_tag="PKM80_03545"
     CDS             6..161
                     /locus_tag="PKM80_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93013.1"
                     /translation="MDRLVMIMTKAESIRDVIAFPKSLKAADLMTQAPSEVEPQQWKE
                     LHLAPTK"
     gene            128..730
                     /locus_tag="PKM80_03550"
     CDS             128..730
                     /locus_tag="PKM80_03550"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNV93014.1"
                     /translation="MERTTSGTHKVAVFCSGLSRGSNLCAIYKYFADNKLPIEIALVI
                     FTCKDAPAIQLAEERNLNYQIIQARDMQSFEKRAMELCRQHSIELIALSGFLKQLSPN
                     FIESMQIPILNIHPALLPKYGGKGMYGMAVHNSVFAAGEKESGVTIHLVNSQYDNGKI
                     VAQEKVDISACKSPEEIAQKVLDIEHKLYARTIKEYLLNH"
     gene            727..1995
                     /gene="mtaB"
                     /locus_tag="PKM80_03555"
     CDS             727..1995
                     /gene="mtaB"
                     /locus_tag="PKM80_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNV93015.1"
                     /translation="MKRIAIITLGCKANQAESSIILNQFEPFKLVSWQEEADIYIINT
                     CTVTNRTDYKSRYLIRQALTKKAKNPSIQIIVTGCYAQRYPEEITKLGNIDFVVDNQQ
                     KLNIADILAGNSYEFMDIMQAKDFACKLTNKMYDRTRAFQIIQDGCNFNCAYCAVPYG
                     RGRSRSATLEQILQQARLFVKNGYKEIVLSGINLGLYKDGDNDLTDVLRCLNDLEGLE
                     LIRISSLEPQLFNYKLINLLPSLPKLCPHFHIPLQSGTDSGLKRMKRHYTTTTIKKLV
                     FDILDKIPSAAIGMDVITGFPGETEEEHKFTCDFICSLPLAYLHTFSYSKRKGTPAAE
                     MTNQIPKKIKNQHSNELIHISQELTANYTNLLYNNNIPLRGIVENSENGCSEFLSDHY
                     VRVYLSKEYPVGELVKTSAKQVKIAGESDV"
     gene            complement(2164..2940)
                     /locus_tag="PKM80_03560"
     CDS             complement(2164..2940)
                     /locus_tag="PKM80_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV93016.1"
                     /translation="MKSQKVILPLLFFIFLIILWQFISAKGVIAFWVVPSPLQVINVF
                     MKNPGLIWHHLKPTLLEAITGLIISIILGSITAMAMNLSSWIKQILYPYLIISQTVPI
                     IAVAPLLIIWFGYGISSKIFAVVLMCFFPIALGLYDGFRSVSVDQIRLLKSMGATPYK
                     IFKLLKIPAALPNFFTGLKLAATYSVMGAVIGEWLGGTSGLGIYMTRATKSYQTAHVF
                     AVIIVIVAVSIALFGMVALLDRILLKWKYQTIDEYLEPIK"
     gene            complement(2937..3878)
                     /gene="murB"
                     /locus_tag="PKM80_03565"
     CDS             complement(2937..3878)
                     /gene="murB"
                     /locus_tag="PKM80_03565"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNV93017.1"
                     /translation="MNITTLSILQQEYPKLMEDGIIQSNVPLKEHCSFQIGGPAEVFC
                     TPYTQNQLLDLLRFCLNHKTPYFILGKGSNLLISDKGLSGIVISTERFTELTVEQEYI
                     TASCGVTLKDLCNYACENGLSGLEFASGIPGSVGGAVFMNAGAYNYEIKDVLHSSVCI
                     LPTWESINSLSPILYLKVEEHNFSYRHSVLQDKELIHLSSVFHLKRDNSETIKRRMDE
                     YQQKRQQKQPLELPSAGSVFRRPEGFYTGRLIEDCGLKGFRIGDAAISEKHCGFIVNL
                     GSATAKDVLQLIQHIQEVVYEHYNVKLQTEIRFLGEM"
     gene            complement(3875..>5564)
                     /locus_tag="PKM80_03570"
     CDS             complement(3875..>5564)
                     /locus_tag="PKM80_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNV93018.1"
                     /translation="THTGDISENSSFDKNYYSVLWHKICSDRYLCGGRKCLNYHNCYV
                     MKLRKHIESASIVVANHSLLLADAQMDNSTLGEYQYLIIDEAHNLMSAASKNLGFTFS
                     YAELNSLFNQLAHSGKKYTGGFLPQLIQTVHKSILTTDQKTHIESLCTKLMLETDEQR
                     KVITSLFNLAANRCFEAKSYNKLRIKDIADFQDIYFFLKELSIGFKNILKDLVALDNV
                     LRTVKSNQIANLEIIKDNLNGYLQRYRETETLLLELLNPDLDNYALWIENIPRPERNI
                     PSSTFCYAPVEVSEPLNRLLYKNIPCIVFTSATLSIRGSFKFFLNQSGLSFATDKKVI
                     ESIVESPFDYDKQSLLLIGSFLPEHKDRFFQSQALGCLEQILTTTNVGTMALFTSYKD
                     LDAVYEHLSDTLYHSKRPFFAQGKGGSRNSILDDFKKSKNAVLLGTSSFWEGVDIQGD
                     SLSLLILYKIPFQVPSEPLVEALIDKLERENKDSFMHLMLPNALLRLRQGFGRLIRSK
                     TDRGIVLIMDSRVSKKKYGQYFKQILPGRCIELKNEQQLISEIGKFFSSLPSNL"
BASE COUNT         1862 a         1014 c          980 g         1708 t
ORIGIN      
        1 caggtttgga tagattagtt atgattatga ctaaagcaga atccatccgg gatgttattg
       61 cttttcctaa atccttgaag gccgcggatt taatgactca ggcaccttcg gaagttgaac
      121 ctcagcaatg gaaagaacta catctggcac ccacaaagta gctgtttttt gcagcggctt
      181 aagtcgcggt tcaaatttgt gtgctatata taaatatttt gcggacaata agttgccgat
      241 agaaatagct ttggttattt ttacttgtaa agatgcgcct gcgatccaat tagctgaaga
      301 aagaaactta aattaccaga taatacaagc tcgtgatatg cagagttttg aaaaacgagc
      361 tatggaactc tgccgccaac atagcattga acttattgct ttaagcggtt ttttaaaaca
      421 gctctctccc aattttatag agagtatgca aatcccgatt ttgaacatcc atcctgcttt
      481 actacccaaa tatggaggta aaggaatgta tggaatggct gttcataatt ctgtgtttgc
      541 ggcgggagaa aaggaatcgg gtgtaacaat tcatcttgtc aattcccaat atgataacgg
      601 taagattgtc gctcaggaaa aggttgatat cagtgcctgc aaatctccag aggaaattgc
      661 ccaaaaagtg ttggatattg agcacaaact ttatgcacgc accatcaagg aatatctttt
      721 aaaccattga aaagaattgc aataattact cttggatgta aagccaatca agctgaatcc
      781 tccatcatct taaaccagtt cgaacctttt aaactggttt cttggcagga agaagcagat
      841 atatatatca ttaatacttg cacagtaaca aaccgaaccg attataaaag taggtatcta
      901 atccgtcagg cgttaaccaa aaaggccaaa aatccttcta tacaaataat agttactggt
      961 tgttatgctc agcgttatcc tgaagaaatt accaaactgg gaaatataga ttttgttgtg
     1021 gataatcaac aaaaattaaa tatagccgat attctggcag ggaatagcta tgaatttatg
     1081 gatattatgc aagcaaagga ctttgcctgt aaactaacca ataaaatgta tgatcgcaca
     1141 cgtgcctttc agattattca agatggctgc aattttaact gtgcgtattg tgcagtccct
     1201 tatggaaggg gaagaagtag gtctgcaacc ttagaacaaa ttttacaaca agccaggtta
     1261 tttgtaaaaa acggctataa ggaaatagtg ctatcgggaa tcaatttagg gctttataaa
     1321 gatggagata acgatttaac cgatgtttta agatgtctga acgatcttga aggtctggaa
     1381 ttgattagaa tcagttctct ggaaccccaa ctctttaatt ataaactaat taaccttctt
     1441 ccctctttac ctaaattgtg cccccatttt catataccct tacaatctgg taccgattct
     1501 gggctaaaaa ggatgaagcg ccattataca actaccacaa ttaagaagtt ggtttttgat
     1561 attttagata aaattccttc tgcagcaata ggaatggatg taataaccgg tttccctggg
     1621 gaaactgaag aagaacataa atttacctgt gattttattt gttctctgcc cttggcatat
     1681 ctgcatactt tcagttattc taaaagaaaa ggaacacctg ccgctgaaat gaccaatcaa
     1741 atcccgaaaa aaattaaaaa ccaacacagt aatgaactaa tccatatttc tcaagagctt
     1801 accgctaatt atactaattt attatataat aacaatatcc ctttgcgcgg aattgtggaa
     1861 aatagcgaga atggttgtag cgagttttta agcgatcact atgttcgcgt ctatttatct
     1921 aaagaatatc cggtgggaga attagtaaaa acttcagcta aacaagttaa aattgctggt
     1981 gaatcagatg tttaatatga ttagtaaaaa aatagaacac agataacggg atcgacagga
     2041 tcagctggat tttagttgag agataagtaa aaataagaaa cctgagtact tttttaatct
     2101 ctttcggcgt ttttagtggc ttttattatc tttatgcttt atatttgtac aatctatgaa
     2161 agcttatttt ataggttcta agtactcatc tatagtttga tatttccact tcagtaaaat
     2221 tctatccaat aaagcaacca ttccaaataa tgcaatacta acagcgacga taactataat
     2281 tacagcaaaa acatgagctg tctgatagga ctttgtagct ctggtcatat aaattcccaa
     2341 gccggaagtt cctcctaacc attcaccaat aacagctccc ataacactat aagttgcagc
     2401 cagttttaat cctgtaaaaa agttaggtaa agcagccgga atctttaata acttgaatat
     2461 cttgtatggt gttgctccca tggattttaa aagacgaatc tgatctacag aaacacttct
     2521 gaaaccgtca taaagtccta aagcaatagg gaaaaaacac ataagtacaa cggcaaaaat
     2581 tttggaactg attccataac caaaccagat aattaaaagc ggtgccacag ctattatagg
     2641 aactgtctgt gaaattatca gatagggata gagaatttgt tttatccaac ttgataagtt
     2701 cattgccata gccgttatcg aacctaatat gatactgatg ataagtccgg ttatcgcttc
     2761 cagcaatgtt ggttttaaat gatgccagat aagccctgga tttttcataa aaacatttat
     2821 tacttgtaag ggtgaaggta ccacccaaaa agcaattaca cctttcgcac tgataaactg
     2881 ccagagtata atcaggaaaa taaaaaaaag caaaggaagt attacttttt ggcttttcac
     2941 atctcgccca gaaagcggat ttcagtttgc agcttaacat tataatgttc atacacaact
     3001 tcttgaatat gctgaataag ctgtaaaaca tcttttgcag ttgcagaacc cagattaaca
     3061 ataaaaccac aatgtttttc ggaaatagca gcatctccta ttctgaaacc tttaagccca
     3121 caatcttcaa ttaacctgcc tgtataaaat ccttccggtc ttctaaatac actgccagca
     3181 ctgggtaatt caagtggttg tttttgctgt cttttttgtt gatattcatc catccgccgt
     3241 ttgattgttt cagagttatc tctttttaaa tgaaacacag aagaaaggtg aattaactct
     3301 ttatcttgca acacactatg acggtaggaa aaattatgct cttccacttt tagatataaa
     3361 atcggactta atgaatttat actttcccaa gtaggtaata tacatacgct gctatgtaga
     3421 acatctttta tttcataatt ataggcaccg gcattcataa aaacggctcc acctactgaa
     3481 ccgggaatgc cagaggcaaa ctctaatccg cttaagccat tctcacaggc ataattacaa
     3541 agatctttta aagtaactcc acagcttgca gttatatatt cttgttctac agttagttct
     3601 gtaaaacgct cggtgctgat cacaattcct gaaagtccct tatcggaaat gagcagatta
     3661 gaacccttac ctaagataaa atagggagtt ttatgattaa gacaaaatct taataaatcc
     3721 agcaattgat tctgtgtgta aggagtgcag aaaacctcag ctgggcctcc aatttggaaa
     3781 ctgcaatgtt cttttaaggg aacattgctt tggataattc cgtcttccat taatttagga
     3841 tactcttgct gtaaaataga taaagttgta atattcatag attactcggt agcgagctaa
     3901 aaaatttgcc aatttcggat atgagttgtt gctcattttt tagctcaata cagcgacctg
     3961 gcaaaatttg tttaaagtac tggccgtatt ttttcttgga aacgcgggaa tccataatca
     4021 ggacaattcc gcgatcggtt ttacttctta ttaggcgtcc aaaaccttgt cttaaacgca
     4081 atagagcatt gggtaacatt aaatgcataa aggaatcctt attttcccgt tccagtttat
     4141 ctattagtgc ttctacgaga ggttctgaag gcacttgaaa aggtatttta tataatatca
     4201 aaagcgaaag tgaatcgcct tgaatatcaa ctccttccca aaaagaagaa gttccaagaa
     4261 gtacagcatt tttacttttc ttaaaatcat ccaagataga atttctgcta ccaccttttc
     4321 cctgagcaaa aaaaggacgc ttagaatgat agagggtatc gctaaggtgt tcgtaaacag
     4381 catccaaatc tttatatgaa gtaaaaagag ccatcgttcc cacattggta gtagttaaaa
     4441 tttgttccaa acaacccaat gcttgacttt ggaaaaatct gtccttgtgt tcaggtagaa
     4501 aactaccaat taataataag gattgtttat cgtaatcaaa aggagattcc acaatgctct
     4561 cgatcacttt cttgtcggta gcaaaactta agcctgattg atttaggaaa aacttgaatg
     4621 aacctctaat tgacaaagtt gccgaggtaa aaactatgca aggaatattt ttgtaaagta
     4681 aacggttaag cggttctgag acttctactg gggcataaca aaaagtactg gaagggatat
     4741 tacgttcggg acgcggaata ttttctatcc ataaagcata attatccaaa tcgggattaa
     4801 gtaattccag caataaagtt tccgtttccc gatagcgttg caaataacca ttcaaattat
     4861 ctttaataat ctccaaatta gctatctggt tacttttcac ggtacgcaac acattatcaa
     4921 gagcaaccaa gtctttaaga atatttttga aacctatgga aagttctttc aagaaaaaat
     4981 agatatcctg aaaatcagct atgtctttta tccttagttt attataactt ttcgcttcaa
     5041 agcagcgatt cgctgctaaa ttaaataagg aagtaatcac ttttcgttgc tcgtctgtct
     5101 ccagcatcag ctttgtgcaa agagattcta tatgagtttt ctggtctgta gttaaaatac
     5161 tcttatgcac tgtttgaata agctgaggca aaaaaccacc ggtatatttt ttcccagaat
     5221 gagctagctg attgaataaa ctgttaagct cagcataact gaaagtaaag cccaaatttt
     5281 tggacgcagc agacataagg ttatgtgctt catcaattat taaatattga tattcaccta
     5341 aagtggaatt atccatttgg gcatctgcta ataatagaga atgattggca actactatgg
     5401 aagcagattc tatatgctta cgcaacttca ttacataaca attatgataa ttaagacact
     5461 ttcttccccc gcaaagataa cgatcggaac atattttatg ccataaaaca ctatagtaat
     5521 ttttatcgaa tgaggaattt tcggaaatat caccggtatg agta
//
LOCUS       DAORHB010000087         5535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_15038_length_5535_cov_4.941241, whole genome shotgun sequence.
ACCESSION   DAORHB010000087 DAORHB010000000
VERSION     DAORHB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15038_length_5535_cov_4.941241"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="PKM80_03575"
     CDS             <1..1598
                     /locus_tag="PKM80_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNV93019.1"
                     /translation="KDPWEEILNQEKSKPDSTNEEMYYDENGYLVLDIVAEENKTETN
                     NKLEIVWEGIEKRFYPIIIAKDNYNFVLDTISDSTFYYVEDANQPNKNNYLKKANIYG
                     KNQKEVFNFGSTANAYCKVNSTVYYLVQGELKSYNLATSSRKDIKVNYDYQYDKALLN
                     QRVFEEAWSAFGNNFYDPDMHNVSWKELFRLYRPYVDKARDIYDVATIIEEMIGDVNA
                     SHTGFYPRSEYSPFRNPVAWLGAEFDYNIILEEGIVLSRVYPNSRLSNFYHLKDGCIL
                     TNVDGVKITSKTSLDSLLAGKIGKKIKLTVVQDGVDIEAVITGLSYSQMYELRYNYQI
                     AQNRKLVDDLSHGKLGYIHIPAMGNDDWKQFYREFLVDNYDKDALVIDVRGNSGGHIH
                     DQIITLLQKKKYAYTATRRYSRILRSEPRSIWDKPSIVLVNEESFSDGEIFPMVYQEL
                     KLGKVVGTPSSGAVIGTWHYYLQDGSSMRMPGTGWYKLDGTNMEGTGVMPDIIILNLP
                     EDIIAERDPQLVRAVKELLKELK"
     gene            1600..2475
                     /locus_tag="PKM80_03580"
     CDS             1600..2475
                     /locus_tag="PKM80_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HNV93020.1"
                     /translation="MNLSDINNLIQRATVLAKTNWLQAVYLLQEGLKEEPDNYSLIIN
                     LGDIYLERQIYEKALSYYIKAISLHPDSPQLLFLIGTCYFSLGEYRIAISYFNRISNP
                     PPEVLYNSALAYAFLGCYQESIDVINKILEVMDDNPFIYFLLIEQYVRMQNYDQAYQT
                     IINAEKKFGKHRQLLLLSALVYGKKGIWLKSYHSFVEYESLGHITNPDHLIAYANAAT
                     NIGLIDRAIEILRQAQEINPYISVVYEELIRLLLQKNNYKEARKVLSLAKKYISQFSP
                     VLYLFKERLDNHPPE"
     gene            complement(2718..3131)
                     /locus_tag="PKM80_03585"
     CDS             complement(2718..3131)
                     /locus_tag="PKM80_03585"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNV93021.1"
                     /translation="MKEQSLLLIKPNAVRNKHIGHIVTILEENDFHFCALKIFRFDRQ
                     LGELFYAEHKGRAFFENLLSFMISDDTVAIIVEKDNAVQELREIIGDVDPEKRKPGTI
                     RALYAEGITENAVHASDSITSAIREIGIIFDKQTN"
     gene            complement(3128..3451)
                     /locus_tag="PKM80_03590"
     CDS             complement(3128..3451)
                     /locus_tag="PKM80_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93022.1"
                     /translation="MLNYEDLQINYENLLKLGYAVVDVRYKDYDVCPDIPKLVIARVD
                     SDRDDFYQDMLKLYTGIEFKPNEMFEIWTDILSHKIKMSIVLHRDIAIKVAALDYIET
                     VYPSK"
     gene            3573..>5535
                     /gene="uvrB"
                     /locus_tag="PKM80_03595"
     CDS             3573..>5535
                     /gene="uvrB"
                     /locus_tag="PKM80_03595"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNV93023.1"
                     /translation="MQRFKLVSDYIPSGDQPEAIKSLVKGIKEDKKYQVLLGVTGSGK
                     TFTIANVIEQINRPTLVLSHNKTLAAQLYGELKQLFPENAVEYFISYYDYYQPEAYIP
                     GKDIYIEKDSSINEQIEKLRLRATMSLMERRDVIIVSSVSCIYGLGVPEEYREALISL
                     SRGMKMDREELIKKLVAAHYSRNDIAFERGAFRVRGDTVDIFPAYLEHCLRVEFFGDE
                     IYRLEKLHPISCNSLGEVENYDVYPAIHFLMNEDKLHKAIINIENEMNERIAWFMQNQ
                     KYLEAERLKQRTAFDLEMLRELGYCSGIENYTRHLTGAKPGEPPNCLLDYFPEDFLFV
                     IDESHVTIPQVHGMFGGDYTRKKNLVEYGFRLPSAFDNRPLRFEEFENYMRNVIFVSA
                     TPANYELELTQGEIVEQVIRPTGLLDPEIEIKPVKHQVDDLIGQIKQRTVKSQKVLVM
                     ALTKRMSEDLSTYLINAGIKARYMHSDIDSIERAKIIRDFRLGVFDVIVGVNLLREGL
                     DLPEVSLVAILDADKTGFLRSTRSLIQISGRAARNVDGKVIFYADEITDAIKQTITET
                     NRRRVKQLAYNKAHNITPQTIYKTIEQIMQSTAIAEGYDKISKPITDEKPSEKDFLEY
                     LDLDNKEKIIELLTKEMNKAAANLEFEHAA"
BASE COUNT         1822 a          955 c         1025 g         1733 t
ORIGIN      
        1 ataaagatcc ctgggaagag attttgaacc aggaaaaaag taaaccggat agcacaaatg
       61 aagaaatgta ctatgacgaa aatggctatc tagttttaga tattgtagca gaagagaata
      121 aaactgaaac aaataataaa ctggaaattg tttgggaagg tatcgaaaaa cgcttttacc
      181 ctattataat agcaaaggat aattataatt tcgtgctgga cacgattagt gacagtactt
      241 tttactatgt tgaagatgcc aatcaaccga ataaaaataa ctacctcaaa aaagctaata
      301 tctacggcaa aaatcaaaag gaagtattca attttggcag cacagctaat gcttactgta
      361 aagtaaatag caccgtctat tatcttgtac agggtgaact taagtcctat aatttggcta
      421 cttcctctcg caaagatatc aaggttaatt atgattatca gtatgataaa gcccttttga
      481 atcagcgagt ttttgaagaa gcatggagtg ctttcggtaa taatttttac gatccggata
      541 tgcataatgt ttcctggaaa gaactcttcc gtttataccg tccttatgta gataaggcaa
      601 gagatattta tgatgtagcc actataatag aagaaatgat aggtgatgta aatgcttcac
      661 atactggatt ttatccacgc agcgaatata gtcctttcag gaatcctgtc gcttggctgg
      721 gagccgaatt tgattataat ataattctgg aagaaggaat tgtcttaagc cgagtttatc
      781 caaattcccg tttaagcaat ttttatcatc tgaaagatgg ctgtattctt actaatgtgg
      841 atggagtgaa aatcacttca aagacatcgt tggattcact gcttgcagga aaaattggga
      901 agaagataaa gcttacagtt gttcaagatg gagttgatat agaagctgtt attaccggtt
      961 tgtcttattc ccagatgtac gagctgcggt ataattatca gatagctcaa aatcgtaaac
     1021 ttgtagatga tctttctcac ggcaaattag gttatattca tattccagct atgggaaatg
     1081 atgattggaa acagttttat cgcgagttct tagtggataa ttatgataag gatgctttgg
     1141 ttatagatgt tcgtggtaat tcaggaggcc atattcatga tcagattatc acactactgc
     1201 aaaaaaagaa gtatgcgtat accgctacgc gtaggtatag tagaattttg cgttccgaac
     1261 ctcgaagtat ttgggataaa ccaagcattg tattagttaa tgaggaatct ttcagcgatg
     1321 gagaaatttt ccctatggta tatcaagaat taaaattagg caaggtagtt ggaactcctt
     1381 ctagtggagc agttattgga acctggcatt attatcttca ggatggttct tcaatgagaa
     1441 tgccgggaac aggttggtat aaacttgatg gtaccaatat ggaaggaaca ggagttatgc
     1501 ctgatataat tatattaaat ttacctgagg atattattgc agagcgtgat cctcaacttg
     1561 tgcgtgctgt taaagaactt ctaaaagagc ttaaatagta tgaatttatc tgatataaac
     1621 aatctcattc aacgggcaac tgtcctggct aaaactaatt ggctacaagc agtttattta
     1681 cttcaagagg gtttgaaaga agaaccggat aattattccc ttatcatcaa tttgggagat
     1741 atctatctcg aaaggcaaat atatgaaaaa gcccttagtt attatattaa agccatttcc
     1801 ttgcatccag atagccccca gttgcttttt ttaattggga cttgttattt ctccttagga
     1861 gaatatagaa tcgcaatcag ctatttcaac aggattagta atccacctcc cgaagttcta
     1921 tataatagtg cattagctta tgcattttta ggttgttatc aggaaagtat cgatgtaata
     1981 aataaaatcc ttgaagttat ggatgataat ccatttatct attttctcct gattgaacag
     2041 tatgtcagaa tgcaaaacta cgatcaagca tatcaaacaa ttattaatgc agaaaaaaaa
     2101 ttcggcaagc atagacagct tttgctactt tctgctttag tttatggcaa aaaaggcatt
     2161 tggttgaaat cctaccacag ctttgtagaa tatgagtctt taggccatat cacaaatccg
     2221 gatcacctaa ttgcctatgc taatgcagcc accaacattg gtctgattga tcgagcaatt
     2281 gaaatattaa gacaagctca ggaaataaat ccttatataa gtgtagttta tgaagagtta
     2341 atccgtctgc tcctgcaaaa aaataattat aaagaagccc gcaaagtact ttctttagcc
     2401 aaaaaatata tatcccagtt ttctcctgtt ttatatctct ttaaagaacg actggataac
     2461 catcctccgg aataataagt gttcccctac tcttttcccg ttcaaattct tgccttgtta
     2521 tttgcggtat agatacgata atttatcttg gcattttacc cagtgaagcc acgatatctt
     2581 tgcagtatat aatcaagtag gaaaacacat agtgtaattt caccaattat tttatatcca
     2641 gtggaaaaca gggctcagaa atatctcttt gttattattc gccttatttt caaatcttgc
     2701 tgaagaattt tactgtgcta attagtttgc ttatcaaata tgattccgat ttctctaata
     2761 gcacttgtaa ttgagtccga agcgtgtact gcgttttcag taatcccttc ggcataaaga
     2821 gctctaattg ttcccggttt tcttttttcc ggatctacat ctccgattat ttcccttaat
     2881 tcctgaacag cgttgtcttt ttcgacaata atagctacag tgtcatccga tatcataaag
     2941 ctcagtaaat tttcaaagaa tgctctaccc ttatgttcag cataaaaaag ttcccccaac
     3001 tgcctatcga aacggaatat tttaagggca caaaaatgga aatcattctc ttccaaaatc
     3061 gttacaatat gccctatatg cttgttgcga actgcatttg gtttaattag cagtagtgat
     3121 tgttccttca ttttgaggga tatacagttt cgatataatc cagagcggca acttttatgg
     3181 caatatcacg gtgtaaaact atactcattt tgattttatg gctgaggata tctgtccaga
     3241 tctcaaacat ttcattgggc ttaaattcaa ttccagtata gagtttcagc atatcctgat
     3301 aaaaatcatc cctgtctgaa tcaactctgg caataaccag tttgggaata tcagggcaaa
     3361 catcgtagtc cttataacgg acatcaacga cagcataacc caatttgagc aggttttcgt
     3421 agttaatttg taaatcttcg taattaagca tttttctatc tccaagctaa ataattctgg
     3481 acaaaacaag ggattcatat tccttgtaaa gcaattcaga attcaagccc tgtaatgtca
     3541 agaagaattt ctaactaaaa aggagacaaa taatgcaacg ctttaaactt gttagcgatt
     3601 atatcccttc cggtgatcaa cccgaagcga taaaatctct tgtaaaagga attaaagaag
     3661 ataaaaaata tcaagtcctt ttaggcgtta caggtagcgg aaaaacattt acaattgcca
     3721 atgttattga acagataaac cgtcctaccc ttgttttatc acataataaa actcttgctg
     3781 ctcagcttta tggtgaatta aaacaacttt tccccgaaaa tgcagttgaa tatttcatca
     3841 gctattacga ttattatcaa cctgaagcat atattcccgg aaaagatatc tatattgaaa
     3901 aagatagcag tatcaacgaa cagatagaga aattacggtt aagagcaaca atgagtttaa
     3961 tggaaagaag ggatgtaata attgtttcct ctgtgtcttg catctatggt cttggtgttc
     4021 cagaagaata tcgtgaagca ttaatttctc tatcgcgggg aatgaaaatg gaccgcgaag
     4081 aattgattaa aaaattggtt gcagctcact acagccgcaa tgatattgct tttgaaagag
     4141 gtgcattcag agttagagga gatactgtag atatttttcc tgcttatttg gaacactgtc
     4201 tacgcgtgga attttttgga gacgaaattt atcgtttgga aaaactgcat cctatctcct
     4261 gtaacagttt gggagaagtt gaaaattacg atgtttaccc cgcaattcat tttttaatga
     4321 acgaggataa attgcataaa gccatcatca atatagaaaa tgaaatgaat gaacgcatcg
     4381 cctggtttat gcaaaatcaa aaatacctgg aggcagaacg tctaaaacaa agaactgctt
     4441 tcgatttgga aatgcttcgt gaactgggtt attgtagtgg tatcgaaaat tatacccgcc
     4501 atctaacagg tgccaaacct ggtgaaccac caaactgttt actggattac ttccccgaag
     4561 attttctctt tgtaattgat gaatcccatg taacaattcc gcaggttcat ggtatgttcg
     4621 gaggtgatta tactcgcaaa aaaaacttgg ttgagtatgg tttccgttta ccttctgcat
     4681 ttgataatcg cccgttacgt tttgaagaat ttgaaaatta tatgcgtaat gtcatttttg
     4741 tttctgccac tcctgcaaat tatgagttgg aattaactca aggcgaaatt gttgaacaag
     4801 ttatccgtcc cactggttta ctcgatccag aaattgaaat taaacctgta aagcaccagg
     4861 ttgatgatct tattggccaa attaaacaac gcacagttaa aagccaaaaa gttctggtga
     4921 tggctctcac taaaagaatg agtgaagacc ttagtaccta tcttattaat gctggtatca
     4981 aagcaagata tatgcacagc gatatcgaca gcatcgaacg cgctaaaatt attagagatt
     5041 ttcgcttagg agtattcgat gttattgttg gagttaatct tctaagagaa ggtttagatt
     5101 tacccgaagt ctcgttagta gctattttag atgctgataa aactggattt ttgcgttcaa
     5161 ctcgctcctt gattcagatt tcgggtcgag ctgctcgtaa tgttgatggc aaagtaattt
     5221 tctatgctga cgaaattaca gacgctataa aacaaactat aaccgaaact aatagacgcc
     5281 gggtaaaaca gcttgcctat aataaagccc ataatattac cccacaaaca atttataaga
     5341 caattgaaca aattatgcaa tcgacagcta ttgctgaagg atatgataag atatcaaaac
     5401 caattaccga tgaaaaacct tcagaaaagg attttttgga atatttagat ctcgataata
     5461 aagaaaagat tattgaactg ctgactaaag aaatgaataa ggctgctgcc aatctcgaat
     5521 ttgaacatgc tgctg
//
LOCUS       DAORHB010000088         5531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_15059_length_5531_cov_3.737947, whole genome shotgun sequence.
ACCESSION   DAORHB010000088 DAORHB010000000
VERSION     DAORHB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15059_length_5531_cov_3.737947"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKM80_03600"
     CDS             <1..358
                     /locus_tag="PKM80_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV93024.1"
                     /translation="LSMFYMVFIGLTGMMTMATTNTLIQSIASIEMRGRVIALYTMAS
                     ASTAPLGSILMGSLSSQFGARYTLIICASVFLLWSLYSLKLAPKFIKGVLRMLVISNN
                     TDVYRSKILAPEINIQ"
     gene            355..1044
                     /locus_tag="PKM80_03605"
     CDS             355..1044
                     /locus_tag="PKM80_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNV93025.1"
                     /translation="MKIFYHLGKRDTDYRSLMHLQKDFILEGTKIPISRVIIAEQTHS
                     KQVHICREIDGGAGFMNKPQIPVVDGLITKIPYQYLLIRTADCYPVILMDNRINVIAA
                     LHCGREGTRKNIVGEAIRIMNSQMYCQLTDIIAIVGAGICHNHYEVSQELYNEFGKSI
                     VSQGLEPVLTKNRHIDLRKTLNAQLLKAGIPEINIEHINDCTFENSCYHSYRREKTNN
                     RQINIVGIIHG"
     gene            1037..2329
                     /locus_tag="PKM80_03610"
     CDS             1037..2329
                     /locus_tag="PKM80_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNV93026.1"
                     /translation="MDKTYTNKEGNLILEIRDEPLSAWLTIKKTGKLIDEYAIIALLE
                     EAGIKSGFEEAVEYAQIHSLEKEFDIPFPVAMCKKDENKTILRYKFNPDILTAPEMEI
                     SYQTLAKLKVFKAGDTVAEYSHNIFEQKGSIYNIFGELIDASSVDPEQAIALAGENVA
                     YDVQNCTYYLLKDGYPYVDEEGHISLLDSITLKASELPSDTIISCPIDLIIEGDLTYA
                     NLHCDANLRIEGEVHSCSIECKKDMLVTGDINASSAKGIIVWGNLECSSIHNSYIVCL
                     NNIHFTNKLENSTVVCDGEIIGAPDRSEICGGLTQAGESITAFQAGSYNNTITEIEIA
                     ISPFYRNLLLILTKDLIRLKENKDINATAIVDLENRIQNYEILLDDKLNQFLNREQKD
                     TKRISIQDISRPPIIYRILKHSYEINKPEKKIEFIEKE"
     gene            2329..3969
                     /locus_tag="PKM80_03615"
     CDS             2329..3969
                     /locus_tag="PKM80_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93027.1"
                     /translation="MKTIKNTLKRIFSIVMLLSFSYTLIALETPFAGDPAISPDGKTV
                     CFVYCDDLWLVPFEGGIPRRITNTTCKEWNPQFSPDGKMIAFSSDRDGQSMIYLMPTE
                     GGISKPVTYESLGLSEWFSDSEHLLCTKYTPRWGISFYKVPIDGSRPVMIAEVATRFA
                     TLSPDNKQIVFQKGGYSRREAYRGSANGELWLLNIETKKYTKLTNTELSEMYPRFSQT
                     GALYYCASDGKNLQINKVNKLSFRKPEKITNLSPFSARDINLARDNERLVFEYFDTIY
                     KYDPTIKTGEKVSPLYIDLSEDEWQDNIVRSYSNNNVGDYTVSINELLVGFTHNYDCF
                     FAPVKGGEVKQISYDHGSISSMQFLDNRRMVISKLDNGIVKLFVATADSIITLSPIEW
                     FGADSLCVSDLGKDKNGRWYFKYADYYSHQKVAIADSGLINIRPVKTPWAVTTNFAFS
                     EDGNYALYGCMRDDNYVKELYLYDVKADTSKRILADDGWLDNLVWMPDNRSILLTRNQ
                     DIYRLDLVPRDEFELDKDPWEEILNQEIYRILLYSKIF"
     gene            complement(4195..>5531)
                     /locus_tag="PKM80_03620"
     CDS             complement(4195..>5531)
                     /locus_tag="PKM80_03620"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNV93028.1"
                     /translation="FAYVNEGYVSPVVNVVKYASPAVVNIEAVGKSQQSSLYDPYFDD
                     FFKRFFGGELPDTQQDVTAIGSGFIFDKAGYILTNYHVVENSSKITVSMADGKKFDAK
                     LVGGDSELDLAVIKIETQTDLPVLELGDSDKIDIGEWAVAIGNPLGLKHTVTLGVVSA
                     VNRRIAKPDGSGYYVDLIQTDAAINPGNSGGPLVNIHAQVMGINTAIINTSEGVNLGF
                     AIPINIAKRFASSIIKDGKFEKAYLGVVVQTVNEELVKALGLKVETGALISDVEKDSP
                     AEKAGIKTGDVIVKLDERVIESNEDLVSYLRAYSANTKANIVINRKGQELKLNVILSS
                     KESKIVTAKKSYFGITVREITADDISEMGLKKDLGGVMVETISSSSQSILGLKTGDII
                     TSIAVNGKESKIATLKDWESVASTVKKDSYVALLVYRKSYGNFYTKFYYRGE"
BASE COUNT         1765 a          960 c         1017 g         1789 t
ORIGIN      
        1 tcttagtatg ttctatatgg tttttattgg tttaacagga atgatgacta tggcaaccac
       61 aaatactctt attcagagta ttgcttctat agaaatgaga ggaagagtga ttgcactata
      121 tactatggct tcagccagta cagctccttt ggggagtata ctgatgggta gtttatccag
      181 tcagtttgga gctcgatata ctttaattat ttgtgcttca gtatttttat tgtggtcatt
      241 gtatagctta aagttagctc caaagtttat taagggagta ttaagaatgc tagtaatcag
      301 caataataca gatgtttatc gttccaaaat tcttgctccg gagattaata tccaatgaag
      361 attttctacc atctaggtaa gagagatacg gattaccgat ctttgatgca tttacagaag
      421 gattttatct tggaagggac aaaaatccct atttcaagag taataattgc tgaacaaacc
      481 cattccaaac aagtccatat ttgtagagaa atcgatggtg gtgcaggttt tatgaacaag
      541 cctcaaattc ctgtagttga tggattaata accaaaatac cctatcaata tttacttatt
      601 agaactgcag attgttatcc tgttatttta atggataacc gtataaatgt tatagcagca
      661 cttcattgcg gtagggaagg aaccagaaaa aacatagttg gagaagcaat cagaataatg
      721 aatagtcaaa tgtattgcca gcttactgat attatagcta tcgtaggtgc aggtatctgt
      781 cataatcatt atgaagttag ccaagaactt tataacgagt ttggtaagag tattgtttct
      841 cagggattgg aacctgtttt aaccaagaac cggcatatcg atttacgcaa aacccttaat
      901 gctcagctat taaaagctgg aattccggaa atcaatattg aacacatcaa tgattgcact
      961 tttgaaaact cttgctatca ttcttaccgc cgggaaaaaa cgaataatcg tcaaataaac
     1021 attgtaggta tcatacatgg ataagaccta caccaacaaa gaaggtaacc tgattttaga
     1081 aattcgtgat gagcccttgt ctgcttggtt aaccataaaa aaaacaggta agcttatcga
     1141 tgaatacgct attattgctt tacttgaaga agcaggtatc aaaagcggat ttgaagaagc
     1201 agttgaatat gcacagattc attctctgga aaaagaattt gatattcctt tcccggttgc
     1261 tatgtgtaaa aaggatgaga ataaaacaat cctacgctat aaatttaatc ccgatatact
     1321 aactgctcct gaaatggaga tcagctatca aacattagcc aaattgaagg ttttcaaagc
     1381 tggagatact gtagcagaat attcacataa tatatttgaa cagaagggga gcatttataa
     1441 tatttttgga gaattgatag atgctagttc agttgatcct gaacaggcaa ttgccttggc
     1501 tggagaaaat gttgcctatg atgttcaaaa ctgtacttat tatcttctaa aagatggcta
     1561 tccttatgta gacgaggagg gtcatataag tcttttagat agcattactc taaaagcaag
     1621 cgaacttcca agtgacacaa ttataagttg tccaattgat ttaataatcg aaggagatct
     1681 tacttatgct aatctgcatt gtgatgcaaa cttgagaatt gagggggaag tacattcctg
     1741 cagtattgaa tgtaaaaagg atatgctggt tacaggtgat ataaatgctt cttcagctaa
     1801 gggcataatt gtttggggaa atttggaatg tagcagtatc cataactcct atatagtgtg
     1861 cctcaataat atccatttta ccaataaact tgaaaattcg acagtagtat gtgatggtga
     1921 aataataggt gcaccagata gaagtgaaat ttgcggtggt ttaactcaag caggagaatc
     1981 aattactgct tttcaagcgg gttcttataa taatacaata accgagattg agatagctat
     2041 aagtcctttt tatcgcaatt tgttgctaat cttaactaaa gaccttattc gtctaaaaga
     2101 gaataaagat ataaatgcta cagctatagt ggacttagaa aaccgcatcc aaaactatga
     2161 aatattgttg gacgataagc tgaatcaatt cctaaacaga gagcagaaag ataccaagag
     2221 aatttctatt caggatattt ccagaccccc cattatttat cggattttaa aacatagcta
     2281 cgaaattaac aaaccagaaa agaaaataga atttatagaa aaggaataat gaaaacaatt
     2341 aagaatactt taaaaagaat cttctcgata gtaatgttat tatcatttag ttatactcta
     2401 atagctcttg agacaccgtt tgcaggtgat cctgctattt ctcctgatgg caaaactgtt
     2461 tgttttgttt attgtgatga cctatggtta gttccttttg aaggaggaat tccccgtaga
     2521 attactaata ctacttgtaa agaatggaat cctcaattta gccccgatgg taaaatgatc
     2581 gctttttctt ctgatcgtga tggacaaagt atgatttatt tgatgcctac cgaaggggga
     2641 atatcaaaac ctgtaaccta tgagagctta ggtctttctg agtggtttag tgattccgag
     2701 catcttttat gcacaaaata cactcccaga tggggaatat ctttctataa agttcctata
     2761 gatggttcca gacctgttat gatagcagaa gttgctactc gttttgctac cttaagtcct
     2821 gataataaac aaattgtttt ccaaaagggt ggttattcaa ggcgggaagc atatcgaggc
     2881 agtgcaaatg gagaactatg gctacttaat attgaaacga aaaaatatac aaaacttact
     2941 aatactgagc tctcagaaat gtatccccgt ttttcacaaa caggagcttt atattattgt
     3001 gcatcggatg gtaaaaattt acagattaac aaggttaaca agctctcttt tcgtaaacct
     3061 gaaaaaatta ctaatcttag ccctttcagt gcccgagata taaatctggc aagagataat
     3121 gagcgtcttg tctttgaata ttttgacact atctataaat atgatcccac tataaaaacc
     3181 ggagagaagg ttagccctct ttatatagat ctttctgaag acgaatggca agacaatatt
     3241 gttagaagct atagcaataa taatgtaggt gattatactg tttctatcaa tgaactgctg
     3301 gttggtttta ctcataatta cgattgtttt tttgctccag tgaaaggtgg ggaggtaaaa
     3361 cagattagtt atgatcacgg ttccatttcc agtatgcaat ttttggataa ccgccgaatg
     3421 gtaattagca aactggataa tggaattgta aaactgtttg tagcaacagc agattccatt
     3481 ataacccttt cccctattga gtggtttgga gcggatagtt tatgtgtttc agaccttggt
     3541 aaagacaaaa atggtcgttg gtatttcaaa tatgcagatt attacagcca tcaaaaagta
     3601 gctattgccg attcaggatt gattaatatt cgaccagtaa aaacaccttg ggctgttacc
     3661 accaattttg ctttcagcga ggatggcaat tatgcattgt atggttgtat gcgcgatgat
     3721 aattatgtga aagaactata tctctacgat gttaaagcag ataccagtaa acgaatttta
     3781 gcggatgacg gttggctgga taatcttgtc tggatgccag ataataggtc tatactgtta
     3841 acccgaaacc aggatatata tcgcctggat cttgttccga gagatgaatt tgaattggat
     3901 aaagatccct gggaagagat tttgaaccaa gaaatctacc gtatcctcct gtatagtaaa
     3961 attttttaaa tatgttattc taaaaaatac ttttaagtat aataaaactt gtttagctaa
     4021 acaatataaa ttttaaacgt ttttcgttaa aaaaaattaa aaaaaattta catttaaata
     4081 aaatacacaa gtaaacaaaa atataaaaaa atttacttaa aaatacaaca agatcttttt
     4141 tggaataaaa taatagcggc atgcttatgc atgccgctaa aaatagatat ttatttattc
     4201 tcctctgtaa taaaacttgg tgtagaagtt tccataactc tttctgtaaa ccaaaagtgc
     4261 gacataacta tcttttttca ctgtggaagc aactgactcc caatctttaa gagtagctat
     4321 ctttgattcc ttaccgttta ctgcgattga ggttattata tctcctgtct taagtccgag
     4381 gatgctctgt gaacttgaac ttatagtttc taccataact cctccaagat ctttcttaag
     4441 acccatctcc gagatatcat ctgctgttat ctcccttacc gttattccga agtagctctt
     4501 tttagctgtt actatctttg attccttgct tgaaagaatg acattcagtt tgagttcctg
     4561 tccttttctg tttataacta tgttagcttt ggtattggcc gagtatgccc taaggtaact
     4621 tacaaggtct tcgttacttt ctataactct ttcgtcaagc tttactatta catcacctgt
     4681 ttttatgcct gccttttctg caggtgaatc cttttctaca tcgcttatca aagctcctgt
     4741 ctcaacctta agtcccagtg cttttaccag ttcttcatta acagtctgta ctacaacacc
     4801 aaggtatgct ttttcaaact ttccatcttt tattatggat gaagcaaacc ttttggcaat
     4861 atttatagga atagcgaatc ctaagttaac tccttcagat gtatttatta tagcggtatt
     4921 tattcccatg acctgtgcgt ggatgtttac aagaggtcct ccgctgttac ctggatttat
     4981 tgcagcgtct gtctgtatga ggtctacata gtaaccggaa ccgtcaggtt tggctattct
     5041 tctgttaaca gcactcacaa ctcccagagt aactgtgtgt tttagtccca gaggattacc
     5101 tatggcaact gcccactcgc ctatgtctat tttgtcagag tcaccaagtt caagaacggg
     5161 aaggtctgtt tgtgtttcta ttttaattac tgccagatcc agttctgaat ctcctcccac
     5221 aagcttggca tcaaatttct ttccatcagc catacttacg gttattttgc ttgaattttc
     5281 aaccacatgg taattggtga gaatgtaacc ggctttgtca aatataaaac ctgagcctat
     5341 tgcagttaca tcctgctggg tatcggggag ctctccgccg aaaaaccttt tgaagaaatc
     5401 atcaaaatat ggatcataaa gtgatgactg ctggctttta cctactgctt ctatatttac
     5461 gacggctggc gatgcatact ttacaacatt taccaccgga ctgacataac cttcatttac
     5521 gtaagcgaac a
//
LOCUS       DAORHB010000089         5490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_15240_length_5490_cov_4.700092, whole genome shotgun sequence.
ACCESSION   DAORHB010000089 DAORHB010000000
VERSION     DAORHB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15240_length_5490_cov_4.700092"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKM80_03625"
     CDS             complement(<1..319)
                     /locus_tag="PKM80_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV93029.1"
                     /translation="MSYIVLARKYRPQSFKEVYAQDHITEILQSAISTNRIAHAYLFT
                     GPRGVGKTSLARILAKSLNCVNGPTITPCNSCSNCAEITAGTSPDVIEIDGASNTSVD
                     DIRE"
     gene            473..1693
                     /locus_tag="PKM80_03630"
     CDS             473..1693
                     /locus_tag="PKM80_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNV93030.1"
                     /translation="MKLEGKVVIAQGGGPTAVINQSLVGVTLEARKFTQVTRVYGALY
                     GVQGIVNEEFVDLTQETTHNLEQIANTPSSALLSTRDKPDEKYCMEIFKVLKAHDVRY
                     FFYIGGNDSADTVRIVNEQATKADYEFRAIHIPKTIDNDLVMNDHTPGFGSAARFVAS
                     AFTGANLDNRALPGVYIGVVMGRHAGFLTASSAMAQKYPDDGPHLIYLPERPFHRDTF
                     LSDVKRVYDLYGRCVIAVSEGIVDDKGIPIVTKLSNNQEKDAHGNIQLSGTGALGDLL
                     SDLVRTKLKIKRVRSDTFGYLQRSFIGCVSDVDQHEAREVGERAAHYAIWYNMDGSIS
                     IQRTGFYSVNYQLENLSEIAGKTRIMPNNFINTEGNGITDDFRYYVRPLLGSGFPVAH
                     RLRAPKVAKLLLER"
     gene            2086..3423
                     /gene="gdhA"
                     /locus_tag="PKM80_03635"
     CDS             2086..3423
                     /gene="gdhA"
                     /locus_tag="PKM80_03635"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNV93031.1"
                     /translation="MTINDFMAKVAAKNPGEPEFLQAVKEVVETVWDVYVNNPRFVKA
                     NILERIVEPERVIIFRVPWMDDKGEVQVNRGYRVQFNSAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTTLPMGGGKGGSDFDARGKSDAEIMRFCQSFMTELFRYIGPNTDV
                     PAGDIGVGAREIGYLFGMYKKLQNEFTGVFTGKGLNWGGSLVRPEATGFGVVYFTEEM
                     LKTKGETIKGKRVAISGFGNVAWGAVQKVNHLGGKVVTISGPDGYILDEDGISGEKID
                     YMVELRASNNDRVKDYADKYPNAKFFPGKRPWEVKVDVAIPCATQNELNGDDARSLIN
                     NGVFCVTEAANMPCTPEAMEIFLNSKILYAPGKAANAGGVATSGLEMSQNSIRLSWSR
                     AEVDEKLHGIMQNIHKACVENGKEADGYVNYVKGANIAGFLKVANAMCDQGIV"
     gene            complement(3593..>5490)
                     /locus_tag="PKM80_03640"
     CDS             complement(3593..>5490)
                     /locus_tag="PKM80_03640"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNV93032.1"
                     /translation="DYEMAAIVHQHLKTKEVEFYLKDGVSSFSRNESGLQVHLQSGRT
                     INADMVLLSIGVRPDSKLAEAAGLELGINKGIRVNSYLQTSDPDIYALGDAIAYPNPI
                     TKMESSTYLAGPANKQARIVADNIVKGNIRKYKGSIGTAIAKVFDITVGSTGVSEKVL
                     KDAGIDYISSIIHTSSHAGYYPDALPITIKIVFNPKDGKLLGAQIVGFDGVDKRTDML
                     AHILKHNETIYEIQEIEHSYAPPFSSAKDAVNIAGMVADNIINGLVNIIHWDDLSSLD
                     KNDTFIIDVRTPDETALGTIEGSINIPLDDIRSRLKEIPENKQIVVFCGTGLRSYFAA
                     RILMQNGFKNVYNLSGGYLTYEYATQKQSNEDIFADDYIGKDDNIYKGKEEKMERDIK
                     TITADACGLQCPGPIMRLKNEMDKLAEGDILEISASDPGFAKDVPAWCKMTGNTLLEV
                     KTEGKIIIARIQKGGGDKISKSLESGNNKTIIVFSDALDRALAAFVIANGAASTGKQV
                     TMFFTFWGLNVIKKPNPPKQKKDMMATMFSIMLPRSSQKLSLSKINFAGMGPVLMRQR
                     MKTKNVDSLESMIMQAKKSGVKMIACQMSMDIMGVEPSELIEGVEVGGVATYLADAEQ
                     SNVNLFI"
BASE COUNT         1603 a         1010 c         1073 g         1804 t
ORIGIN      
        1 attctctgat atcatccaca ctggtattag acgctccgtc gatttctatt acatcggggg
       61 aagttcctgc tgttatttct gcacaattgg aacagctatt acagggagtg atagttggtc
      121 catttacgca atttaaactt ttggcaagaa ttcgagccaa agaagtcttc cccacacctc
      181 taggtccggt aaataaatag gcatgggcta tgcgatttgt agaaatagcg ctttggagaa
      241 tttccgttat atgatcttga gcatagacct ctttaaagct ttgggggcga tattttctgg
      301 ctaaaactat gtaactcatc tatcctctca tacatttgtt tttaattttt acaattaacc
      361 attattccca atgcccattt ttaagcaagt attttctgct ggacagatta ctggctctct
      421 gttttaattg ttttgtaaat ataattactt agtccaattg gaggatttta aaatgaaact
      481 ggaaggaaaa gttgttattg cccaaggagg aggtcctaca gctgtgatta atcaatcgct
      541 ggtaggagta actttggaag cacgtaagtt tacacaggtt acccgtgttt atggagcact
      601 ttatggagtt cagggcatcg ttaatgaaga gtttgtagat ttaacccagg aaacaactca
      661 caatttggaa cagattgcca atacaccttc ttctgcatta ctctctacgc gtgataaacc
      721 tgatgaaaag tactgtatgg aaatctttaa ggtcttaaag gcacacgatg taagatactt
      781 tttttatatt ggaggcaacg attctgcaga tacagttcgt atagtaaatg aacaagcaac
      841 taaagcggat tatgaattca gggctattca tattcctaag actatcgata acgatttagt
      901 tatgaatgat catactcccg gttttggctc tgcggcaaga tttgttgctt cagcttttac
      961 tggtgccaat ttagataatc gcgctttgcc cggagtatat attggtgtgg ttatggggcg
     1021 gcatgctggt tttttaactg cttcctctgc gatggcacaa aaatatcctg atgatggacc
     1081 tcatcttatt tatttacccg aacgtccttt ccatcgggat acatttctaa gtgatgttaa
     1141 aagagtttat gatctatacg gcagatgtgt gatagctgtt tctgaaggta ttgtagatga
     1201 taaagggatt cctattgtaa ccaagctttc taacaatcaa gaaaaggatg ctcatggtaa
     1261 tattcaactt tcaggaacag gagcattagg agatttactt agtgatttgg ttcgcacaaa
     1321 attgaaaatt aaacgggtgc ggagtgatac ttttggttat ttacaacgtt ctttcatagg
     1381 ttgcgtaagc gatgttgatc agcatgaagc acgcgaagtg ggtgaacgtg cagctcatta
     1441 tgctatctgg tataatatgg atggttcaat ttcaattcag agaaccggtt tttactctgt
     1501 aaattatcag ctggaaaatc taagtgagat tgctggaaaa acaagaatta tgcccaataa
     1561 cttcattaat actgaaggaa atggcattac tgatgatttt aggtattatg taagaccatt
     1621 gctgggatca ggatttcctg tggcacatcg cttaagagct cctaaagtag ctaaactgct
     1681 attggaaaga taggtggaca gttttagaga tatcgggata tataattatg ataaagacga
     1741 gctttatttg cctcagatag gatctaaatt ctgaagtgat tactgtgaat ataccattta
     1801 aacattccga taattcctat agagagaatt actcataatt gcttcatctt gtttcaggtt
     1861 gcattattat ggcaattaag agtgatgtac tccttttatt tacttaacga tagcgttcgt
     1921 cgaaaattgt tttccgcttt tttagctaat tatttattca gaattttaca ggatcgcaat
     1981 cctgtattat tgctgcccta tcttataatc tttttttctc acttgacata aaatgatagc
     2041 tatatttaaa ggtatataaa ataaaaatca gttatggagg aactaatgac catcaatgat
     2101 tttatggcca aagtcgcagc caagaatcct ggcgagcctg aattcttaca ggcagttaaa
     2161 gaagttgtgg aaacagtttg ggatgtttat gtaaacaacc ctcgtttcgt gaaagccaat
     2221 atccttgaac gtattgtaga gcccgaacgg gtcatcattt tccgtgtccc ttggatggat
     2281 gataagggtg aagttcaagt aaatcgcggt taccgcgtac aatttaacag tgcaattgga
     2341 ccctataaag gtggattgcg ttttcatcca agtgtaaatc tttccattct taagttcttg
     2401 ggttttgagc agattttcaa aaacagctta accactcttc ctatgggagg aggtaaaggt
     2461 ggaagtgatt tcgacgccag aggaaaaagc gatgccgaaa taatgcgttt ctgtcaatct
     2521 tttatgacag aactgttccg ttatattgga cccaacactg atgtcccagc tggtgatatt
     2581 ggtgtaggag ctcgtgaaat tggttacctt tttggaatgt ataaaaagtt acaaaatgaa
     2641 tttactggtg tttttactgg caaaggtctt aattggggtg gaagtttagt ccgtcctgaa
     2701 gccacaggtt ttggtgtggt ttatttcacg gaagagatgc tgaagaccaa aggggaaaca
     2761 attaaaggta agagagtagc catttccggt tttggaaatg ttgcttgggg tgccgtacaa
     2821 aaagttaatc accttggtgg aaaagttgta actatctctg gtcccgatgg ttatatttta
     2881 gatgaagatg gaatcagtgg tgaaaaaatt gattatatgg ttgaattgcg tgcttctaat
     2941 aatgaccgtg taaaagatta tgcagataaa tatcctaacg caaaattctt ccctggaaag
     3001 cgcccctggg aagtgaaagt tgatgttgct attccttgtg ctacacaaaa cgaacttaat
     3061 ggagacgatg caagatcact gattaataat ggtgttttct gtgttacaga agcagctaat
     3121 atgccttgta ctcctgaagc aatggaaatt ttcttgaatt ccaaaattct ttatgctcct
     3181 gggaaagctg ctaatgcagg tggtgtagcc acaagcggat tagaaatgag ccaaaattct
     3241 attcgcctta gctggagcag agcagaagtt gatgaaaaac ttcatggaat tatgcaaaat
     3301 atccataaag cttgtgtgga aaatggtaaa gaagccgatg gctatgtaaa ttatgttaag
     3361 ggtgccaata ttgctggatt cctgaaagta gccaatgcta tgtgtgatca gggtattgtg
     3421 taaaccccct aatctctcta aaatagataa agcccgtatc ggtaagtcgt tacgggcttt
     3481 tttgtattga tttagtcttc ttctatcata ataaaggtgt aaaggttact atatcagttt
     3541 tttacacctt tttcgttgac ttcaagagac cgagagaaaa gtatcataat cgtcaaataa
     3601 acagattcac attagattgt tctgcatccg caaggtaagt agctactccc ccaacttcta
     3661 ctccctcaat caattcactt ggttcaaccc ccataatatc catactcatt tggcaagcga
     3721 tcatttttac tccagatttt tttgcttgca ttatcatcga ttcgagagaa tccacatttt
     3781 tagttttcat tctttgtctc attaaaacag gacccattcc tgcaaagttt atttttgaca
     3841 aacttagttt ttgagaagag cgcggcagca taatactaaa cattgttgcc atcatatctt
     3901 ttttctgttt aggcggattt ggttttttaa taacatttaa gccccagaaa gtgaagaaca
     3961 tcgttacttg tttaccagtt gaagcagctc cattggcaat tacgaaagca gccaaagctc
     4021 tatctaaagc atcactgaag acaattatgg ttttattatt tccactttcc aagctcttgg
     4081 atattttatc accaccccct ttttggattc tggcaattat tattttacct tccgttttca
     4141 cttccaaaag ggtgttgcca gtcattttac accaggcagg aacatctttg gcaaatcccg
     4201 ggtcgctggc agaaatttcc aaaatatcgc cttccgccaa tttatccatt tcgtttttta
     4261 aacgcataat aggtcccgga cattgtaaac cgcaagcatc cgcagttatt gtttttatat
     4321 ctctttccat tttttcttcc ttgcctttat agatattatc atctttccca atataatcat
     4381 ccgcaaagat atcctcgtta ctttgtttct gagtagcgta ttcataagta agatatcctc
     4441 cggagagatt ataaacattc ttaaagccgt tttgcattaa aatgcgcgcg gcaaaatatg
     4501 aacgcagacc ggttccgcag aaaaccacta tttgtttatt ttcaggtatt tccttcaacc
     4561 ttgaccgtat atcatccaaa gggatgttta tacttccttc tattgttccc aaagcagttt
     4621 catcgggagt tcgcacatca atgataaagg tatcattttt atccaaggaa ctcaaatcat
     4681 cccaatgaat aatatttact aaaccgttga taatattatc agctaccatt ccggcaatat
     4741 ttactgcatc tttagcgctg gaaaatggag gagcataact atgttcaatt tcttgaattt
     4801 catagatggt ctcgttatgt ttcaatatat gagccagcat atctgtcctt ttatctacgc
     4861 cgtcaaaacc aacaatttgt gcacccagca gtttcccatc tttaggatta aaaacgattt
     4921 ttatagttat gggcagagca tcaggatagt aacctgcatg agaagaagta tgtatgatgg
     4981 aagaaatata atctattcct gcatctttca gaactttttc cgatactccg gttgaaccga
     5041 cagtaatatc aaaaactttg gcaatggcag ttccaatact gcctttatat tttcggatgt
     5101 tacctttaac gatattatca gccacgatgc gtgcctgttt atttgcaggt cccgcgagat
     5161 aagttgagct ttccattttg gtaatcggat tgggatacgc aattgcatct cccaaagcat
     5221 aaatatcagg atcgctggtt tgcagataag aatttactct tattcctttg tttattccca
     5281 attccaaacc agctgcttct gccagtttac tatcaggacg aactccaatg gataataata
     5341 ccatatctgc atttattgtt ctcccactct gtaaatggac ttgcaagcca ctttcatttc
     5401 tactaaagga tgagacacca tctttaaggt aaaactccac ttctttcgtt tttaagtgtt
     5461 gatggacaat agccgccatt tcataatcca
//
LOCUS       DAORHB010000090         5447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_15397_length_5447_cov_4.276150, whole genome shotgun sequence.
ACCESSION   DAORHB010000090 DAORHB010000000
VERSION     DAORHB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15397_length_5447_cov_4.276150"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..463
                     /gene="queD"
                     /locus_tag="PKM80_03645"
     CDS             92..463
                     /gene="queD"
                     /locus_tag="PKM80_03645"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNV93033.1"
                     /translation="MFYLNVIDTFSAAHRLCGYQGPCSNLHGHNWKVRVCVKTEHQDE
                     IGMSLDFGFLKSMLSAILKDLDHRFLNEVEELKNINPTSENLARFIYERMDEALIDKP
                     VTIYEVEVCESDRSSVIYKNA"
     gene            456..1067
                     /gene="yihA"
                     /locus_tag="PKM80_03650"
     CDS             456..1067
                     /gene="yihA"
                     /locus_tag="PKM80_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNV93034.1"
                     /translation="MLRFVESFFVKSAIEPSDYPASAYPEFAFAGRSNVGKSTLINLL
                     TNHKGLAKTSSTPGKTRLLNFFLIRYKIDETDSSGFLQFTDLPGYGFAEVSQAEQEKW
                     RRMINRYLEQRNQLRALIVLVDIRHPADSKDVMLLEMLRVRRIEYSIVATKADKIPKT
                     KIDKTVKDLAKGLGLQEEPIFPVSALNNTGMEPLYNWIQDKLS"
     gene            1069..2916
                     /gene="mnmG"
                     /locus_tag="PKM80_03655"
     CDS             1069..2916
                     /gene="mnmG"
                     /locus_tag="PKM80_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNV93035.1"
                     /translation="MQTSNKFDLIVVGAGHSGIEAALAAAKRGLKVALFTIKLEAIGR
                     MSCNPSIGGPAKGHLAREIDALGGELAKSADLSGIHFRMLNRSKGPAVWAPRSQNDRQ
                     QYHLLMREAVEKQQNIHLMEAIIEEIIVEQGKVKGVISQIGCYYYAPKVILATGTFLK
                     GKIHIGKISYSGGRSGEPSAEELSDSLSAMGLKVRRFKTGTPPRIDLRSLNYNILEEQ
                     PGDLNPQGFSYYRDIELKNLISCYITHTTEETHKIIRDNINKSALYSGIIKGIGPRYC
                     PSIEDKIVKFPQRESHHIFIEPEGINTFEGYLNGVSTSLPADIQEKIVHSIPGLEEAR
                     ILRYAYAIEYDFIDPEEIDNSLECKKIKGLYLAGQINGTSGYEEAAAQGLMAGINASL
                     ALETKDPLIFKRSDAYLGVLIDDLVTRGTNEPYRMFTSRAEYRLLLRQDNADERLMPI
                     GYKLGLVEESRWQRFMHTQEIYQRELEYLKQHNCMAKGEIKEPIRYAQLLKRPEISFT
                     DLPKFGYHIPEDVNADIQNRLELEIKYSGYLNRMEEEIKRFKTAENIAIPKELDYFQI
                     PSLAYEAREKLSKIRPKNIGQAMRIPGINYSDSVALMIWLHKNAKKEAK"
     gene            2913..3635
                     /gene="kdsB"
                     /locus_tag="PKM80_03660"
     CDS             2913..3635
                     /gene="kdsB"
                     /locus_tag="PKM80_03660"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNV93036.1"
                     /translation="MNVIAIIPARFASTRFPGKPLAMLKGKPIIQSVYERVVNSGLFT
                     SVCIATDDLAIIDAVKRFGGRAVLTSDKHQSGSDRIAEALQYFPKAELVLNVQGDEPL
                     IEKEPLQKLISVFEDREVQMASLMTPLQDLEMLTNPNIVKVITDRQNNALYFSRSAIP
                     YNRDNLSDVTYYRHIGVYAYLPDCLKKFVSLKQGKLERIEKLEQLRALENGISIKMVE
                     TSYQGIGIDTPADLATVEKMLS"
     gene            3691..5163
                     /locus_tag="PKM80_03665"
     CDS             3691..5163
                     /locus_tag="PKM80_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNV93037.1"
                     /translation="MNRYKQHLLFWAFITTALFANAVPITIFHTNDTHGAYLPQNSKN
                     GKIGGYANLEYYLNAEREKASRSLYLDAGDQQTGSIFASQKYNNAIGGAVIKVFNSLN
                     LDATTYGNHEFDYSLNNTIKLRKLANYPFISTNILDESKKPFGEIPYKIFKLDSLKVG
                     VLGLTLTDLPEKVKRENVAELTILPYKEAIDKYIEEVDKQSDLLILLTHNGFAADSLL
                     ATELDDRVDLIIGGHSHTVISEPCKVNGIYLASTGAYLNYLGAINLEVQNDHITSYES
                     CLIPLFAPENLPETPLNRFVSAMADSLDIETGKVIATIPEDWVPDKFKETAVSRWAAE
                     ALKNEYYDIYKPDLAIINNGGIRKIIPAGAVTLKDMYELFPFNNYIVLFSCYGKDLLT
                     MESLNREIAENKPYDIVQTSSTQWKKKKRLLGLIKPKELYYINGKQVDPDKIYRVVSF
                     DYILGQWDKYLGFKPFDIQETGDLFTEVMIRQIKKQFSHR"
BASE COUNT         1803 a          917 c         1105 g         1622 t
ORIGIN      
        1 tattgtgatg ggactatcaa ttataatatc gttagttttg gtgtttatct ttattcataa
       61 gaagtacttt taataatccg ggagaggagc aatgttttac ttaaatgtga tagatacatt
      121 ttctgcagcc catcgtcttt gcggttatca aggtccttgc agtaatttgc atggtcacaa
      181 ttggaaggtt cgggtttgcg tgaaaacaga acatcaagat gaaattggaa tgtctcttga
      241 ttttggtttt ctaaagagta tgttatcggc tatattgaaa gaccttgacc accgtttttt
      301 gaacgaagta gaagagctaa aaaatatcaa tcctacttct gaaaacttgg ctagattcat
      361 ctatgagcga atggacgaag ctctaattga taaaccagta actatctatg aagttgaggt
      421 ctgcgaatct gaccgcagca gtgtgatata caaaaatgct tagatttgta gaatctttct
      481 ttgtaaaaag tgccattgag ccaagtgatt atccagcttc tgcctatccg gaatttgctt
      541 tcgcgggtag aagcaatgta ggtaaatcta cacttattaa tttactaact aatcataaag
      601 gccttgctaa gacctcttcc actccaggaa aaacgcgttt gcttaatttt tttcttattc
      661 ggtataaaat agacgaaact gatagttctg gttttttaca atttacggat ttaccaggtt
      721 atggttttgc ggaagttagc caagcagaac aagaaaaatg gagaaggatg attaatagat
      781 atcttgagca gcgtaatcaa ttgcgtgcct taattgtgct cgtggatatc cgtcatcctg
      841 ctgatagcaa agatgttatg ctattagaaa tgttacgggt aagaagaata gaatactcaa
      901 ttgttgcaac caaagcagat aagattccca aaaccaaaat tgacaagaca gttaaagacc
      961 tggcaaaagg tttaggatta caagaagagc cgatttttcc tgtctcggct cttaataata
     1021 caggtatgga accgctttat aactggattc aggataaact aagctaagat gcaaaccagc
     1081 aataagtttg atctcatcgt tgtgggtgca ggtcattctg gaattgaagc agctttagca
     1141 gctgccaaaa gaggcttaaa agtagctctg tttacaatta aactagaagc tataggcaga
     1201 atgagctgta atccatctat tggcggtccc gctaaaggtc atttggcaag agaaattgat
     1261 gctttaggtg gtgaattagc aaagagtgca gacctttctg gaatccattt tagaatgtta
     1321 aatagaagta agggtccggc agtttgggct ccgcgttctc agaatgaccg tcaacaatat
     1381 catctactta tgcgcgaggc agttgaaaaa caacagaata tccatttaat ggaagctatt
     1441 attgaggaaa taattgtaga gcagggaaaa gttaagggag taatcagtca aatcggttgt
     1501 tactattatg ctcccaaggt tattctggca acgggaactt ttctgaaggg gaaaatacat
     1561 ataggcaaaa tcagttattc gggaggacgg agtggagaac cttctgccga agagctttcc
     1621 gattcactat cagcaatggg attaaaagtt cgcagattta aaactggtac ccctccccgg
     1681 atagacctgc gatcattaaa ttataatatc ttagaagaac aaccaggtga tcttaatcct
     1741 cagggttttt cttattacag ggatattgaa ctaaagaatc ttatttcttg ctatataacg
     1801 catactacgg aagaaacgca taaaattatt agagataata ttaataaatc agctctttat
     1861 tctggcatta ttaaaggtat tggaccgcgt tactgtcctt cgatagagga taaaatagta
     1921 aaattcccgc agcgtgaaag ccatcatatt tttattgaac cggaaggtat taacactttt
     1981 gagggttatc tgaatggggt ttctacttcg cttccagcag atattcagga gaaaattgtg
     2041 cacagtattc ctggtttaga agaggcgcgt attttacgat atgcttatgc catcgagtat
     2101 gattttatcg atcccgaaga aatagataat tcgctggaat gtaaaaaaat aaagggttta
     2161 tatttggcag gacaaattaa tggcacttct ggatatgaag aagcagcagc ccaaggtctt
     2221 atggcaggca taaatgcgtc tttggcttta gaaactaaag acccacttat atttaagcgg
     2281 agtgatgctt acctaggtgt tttgattgat gatcttgtta ccagaggaac taatgaaccc
     2341 taccgaatgt ttacatcgcg agctgaatat cggttacttt taagacagga taatgcagat
     2401 gagcgtttga tgcctattgg ttacaaactg ggtttagtgg aagaaagccg ttggcaaaga
     2461 tttatgcata cccaggaaat ttatcaaagg gaactggaat atttgaaaca gcataactgt
     2521 atggcaaagg gagaaataaa agaaccgatc cgctatgcgc aattattaaa acggccggaa
     2581 atttcattta ccgatcttcc taaattcggt tatcatatac ctgaagatgt aaatgccgat
     2641 atccaaaatc gtcttgaact ggaaattaaa tattcaggat atttaaatcg gatggaagaa
     2701 gaaattaagc ggtttaaaac agctgaaaat attgccatac ctaaagaact ggattatttc
     2761 cagattcctt cccttgccta cgaagcccgc gaaaaattaa gtaaaattcg tcctaaaaat
     2821 attggccaag caatgcgcat tcctggaatt aattatagtg atagcgttgc cttaatgata
     2881 tggctacata aaaatgctaa aaaggaagca aaatgaatgt aattgccatt attccagctc
     2941 gttttgcctc aactcgtttt ccagggaagc ccttagcgat gctgaaaggg aaacctatta
     3001 ttcaatccgt ttatgaaaga gtagtgaatt ccggtttatt tacttctgtt tgtatagcta
     3061 cagacgattt agctatcata gacgcagtaa aaagatttgg aggtagagca gttttaactt
     3121 ccgataagca tcaaagtggt tctgatcgta tagctgaagc attacaatat tttcctaaag
     3181 cagaattggt attgaatgtt cagggtgatg aacccttaat tgaaaaagag cctctgcaaa
     3241 agttaatttc cgtgtttgaa gatagagaag tgcaaatggc cagtttgatg acccctttac
     3301 aggatttgga aatgttaacc aatcccaata ttgtgaaagt tataacggat aggcaaaata
     3361 acgctctgta tttttcccgt tctgctattc cttataatcg ggataaccta tctgatgtaa
     3421 catactatcg tcatattggg gtatacgctt atcttcctga ttgcttaaag aaatttgtat
     3481 ctttaaaaca aggaaaactg gaaagaattg aaaaattgga acagcttaga gctctggaaa
     3541 atgggatttc gataaaaatg gtagaaacga gctatcaagg catcggaatt gatacacctg
     3601 ccgatttggc aacggtagaa aaaatgcttt cttaacgcta caatataatt ttaacctata
     3661 tatatcaatg gaacaacaat ggagaatact atgaatagat acaagcaaca cttacttttt
     3721 tgggctttca taactactgc cttatttgcc aatgcggttc caataaccat ttttcacact
     3781 aacgacacgc atggtgccta tctaccgcaa aattcaaaaa atggcaaaat tggcggttat
     3841 gccaatctgg aatattatct gaacgcagaa agagaaaagg ccagccgatc tttatattta
     3901 gatgccggag atcaacaaac gggcagcata tttgcctcgc agaaatataa taatgccatt
     3961 ggcggagcag ttataaaggt ctttaattct ttaaatttgg atgccacaac ctacggtaac
     4021 cacgagtttg actattcgtt aaataataca attaaattac ggaaattggc aaattatcct
     4081 tttatcagca ctaatatact ggatgagagt aaaaagccct ttggagaaat cccctacaaa
     4141 atatttaagc tagattctct aaaagtaggt gttttgggct taactttaac agacctgccg
     4201 gaaaaagtga aaagagaaaa tgtagccgaa ttaactatcc ttccctacaa agaagcaata
     4261 gataaatata ttgaagaagt tgataaacag agtgatttac ttattttact tacccataac
     4321 ggctttgccg cggattcgct tttggccaca gaattggatg accgcgtgga tttaattatt
     4381 ggaggtcatt cccatacagt tatttccgag ccctgtaaag tgaatggaat ttatcttgcc
     4441 tctacgggtg cttatttaaa ttatttggga gcaattaatc tcgaagtaca aaatgaccac
     4501 atcacttctt atgaaagctg tcttattcca ctttttgcac cggaaaattt accggaaaca
     4561 ccattaaata gatttgttag cgcaatggca gatagcttgg atatagaaac aggaaaagta
     4621 atcgcgacca ttcctgagga ctgggttcct gataaattta aggaaacagc ggtctccaga
     4681 tgggctgcgg aagccctaaa aaatgaatat tatgatattt ataaacctga tttggctatt
     4741 attaataatg gtggcattcg caaaattatt cccgcgggag ctgttaccct aaaagatatg
     4801 tatgaacttt tccccttcaa taattatata gttctgtttt cctgttacgg aaaagatcta
     4861 ttaactatgg aatccctaaa tagagaaatt gctgaaaata aaccctatga tatagtgcaa
     4921 accagttcca cacaatggaa aaagaaaaaa cgccttttgg gtttgataaa gcctaaagaa
     4981 ctctattata taaatggaaa acaagttgat ccggataaaa tttaccgggt ggttagcttt
     5041 gattatattc taggacaatg ggataaatat ttgggattta agccatttga tattcaggaa
     5101 acgggagatc tgtttaccga agtgatgatc agacagataa aaaaacagtt ctctcacaga
     5161 taacacagat gacgcagatc tgatttatcg aaacaaggat tttaggatta gttcacgcag
     5221 attacgctga tgacgcagat ttaagtttta taaacaagga ttttaggatt agttcacgca
     5281 gattacgctg atgacgcaga tctgatttat cgaaacaagg attttaggat tataggattt
     5341 agaggattta tagtttaagt cccagcaggg cttatatctt tattcaaatt tctttcgagg
     5401 ggcttacacc accatcaata taactgtatc ggcctttgag ttaaaat
//
LOCUS       DAORHB010000091         5331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_15933_length_5331_cov_5.066149, whole genome shotgun sequence.
ACCESSION   DAORHB010000091 DAORHB010000000
VERSION     DAORHB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15933_length_5331_cov_5.066149"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKM80_03670"
     CDS             complement(<1..586)
                     /locus_tag="PKM80_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNV93038.1"
                     /translation="MNFKHTLVIFRKELMEVLRDKRTIFTTFILPIILYPLIIVGFNS
                     VMMRQTKVLEERGATIAVQDSVDNEISHRLIQGLVRIKNYTFLPYNESTKQHYAEKDI
                     QCIVTIRDSLGSEGTQFFKIYITYDKSKDQSRIVYEKLSKQLSKVEKELQQERLQISG
                     LSPDFLNLVDIRERDTSSAQKKMGMFLGMFLPYIV"
     gene            complement(576..1343)
                     /locus_tag="PKM80_03675"
     CDS             complement(576..1343)
                     /locus_tag="PKM80_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV93039.1"
                     /translation="MIKVDNLSKVFIKKDGTNFYAVNKINFEAGDGEIVCLLGVNGAG
                     KTTTMRVLSTIFQPSEGSAEIQGWDIRKHPDKVRENIGFLSGDTGLYNRLSCREFITY
                     FGHLYSIEDQLIEQRIKVMADLLDMNEFLDKKIEFLSSGMKQKVSIVRSIIHDPPVMI
                     FDEPTAGLDILTARNIVSFIRDCKQRGKCVLFSTHIMREAERLADRIVMIHKGKILAD
                     GTLEELRNISSQIDLDDIFVYYINQHTDEQELRANEF"
     gene            1903..2799
                     /locus_tag="PKM80_03680"
     CDS             1903..2799
                     /locus_tag="PKM80_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV93040.1"
                     /translation="MKEKATLILGGGSALGLAHIGVLSILEEEFEITGIIGSSMGSII
                     GGLYSSGLSSAKIYELAGSFNNAKLFSPFKLDKTIKGIFDGRSLLNLFLTWTEGKSIE
                     ESEIPFIACAYDLTSKSTVLFNKGLYADAMRASSSLPVLFAPYKLNQYIFVDGGIEHP
                     LPMAFSSLLSEDKVIAVNVLPEASRSASFINLESEEETKTKRLSRTEVIFNAVLQNQA
                     YLAIRDIAMFSPDIVIEAGMPNGRPFAFHKAREFYNFGRHQAMETLANYKEPRFMPTI
                     RKHYRNLIQHLPNLNIPVKDNT"
     gene            2932..3657
                     /locus_tag="PKM80_03685"
     CDS             2932..3657
                     /locus_tag="PKM80_03685"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV93041.1"
                     /translation="MNEIIKILSKVDGGNILDAATGKGDFINTLKQHLKSFTQIIGVD
                     TSQRCVDYAQKIFPENNIEIYRMNLEDMQFEDGYFDTVCISDALHHIKNREAVLNEML
                     RVLKPEGLMLITEMYSDGDQTPAQETHIMMHHWVASVDRLLGTYHQKTFTRQELQDIV
                     KKLPLKKVEIADFYVPVDDPSKNCPALIRNCQDIIKRLQTMENTEAIIAEGKELMERI
                     KDIGYAGACRLAITGYKKKISYN"
     gene            3680..4939
                     /gene="rho"
                     /locus_tag="PKM80_03690"
     CDS             3680..4939
                     /gene="rho"
                     /locus_tag="PKM80_03690"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNV93042.1"
                     /translation="MLFEEDLFSLSQLQLSRLAKKIGLPGYTQYKGTELIFKLLEFQA
                     AQEGLAFVTGCLEIMEDGFGFLRFPDNNYNAGRDDVYVSLTQIRRFGLKTGNMISGPV
                     RSPKEGEKYYALLRVEAVNYLEPNSMLNLRSFDELTPYYPTERLNLEFDPNNFSTRII
                     NLFTPIGKGQRGLIVAAPRTGKTTLLQDTANAILSNHPEVYLIVLLVDERPEEVTEMK
                     KILKPGNREVISSTFDESPKNHSAVSEMVLEKAKRMVELNQDVVIVLDSITRLARAYN
                     NITPSSGKVLSGGIDANGLIKPKKFFGSARNTEEAGSLTIIATALIDTGSKMDQVIFE
                     EFKGTGNMELVLDRSISDMRLYPAIDLVKSGTRREELLLSQNELNRMYVLRKYLKTIS
                     PIQGIETLRKKMQATDTNEELLNSMSN"
     gene            4939..>5331
                     /locus_tag="PKM80_03695"
     CDS             4939..>5331
                     /locus_tag="PKM80_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93043.1"
                     /translation="MIPTRYSVIKRILFSFILISLCTVVYSAVRKEFQELNNLYSKGK
                     LDELSEAISNLKPVNDEERACLGYYNAMLKTKTEDTISAHQWLIERFPKSPYAQKSLL
                     ELGKIYILERKIEEATVYLRRINSTDLIE"
BASE COUNT         1782 a          939 c          937 g         1673 t
ORIGIN      
        1 taactatata gggtaaaaac atccccaaaa acatccccat tttcttctga gcgctggaag
       61 tatctcgttc tctaatatct actaaattca aaaaatccgg gcttaatcca gaaatctgta
      121 aacgctcctg ttgcagctct ttttctactt tggaaagctg tttagaaagc ttttcatata
      181 ctattctact ttgatcttta gatttatcgt atgttatata tattttaaaa aactgggttc
      241 cctcgctgcc taaagaatct cgaatagtaa ctatacattg aatatctttt tctgcataat
      301 gctgttttgt gctttcgtta taagggagaa aagtgtaatt cttgatgcgg actaaaccct
      361 gaatcaagcg atgcgaaatt tcattatcta cactatcctg gacagcaatg gtagctcccc
      421 tttcttctaa cactttagtc tgacgcatca taactgaatt aaaaccaaca attataagtg
      481 gatataatat aatgggaaga atgaaagtag taaaaatagt ccttttatcc cgcaaaactt
      541 ccattaactc ttttcggaag attactaaag tatgtttaaa attcattggc tctcagctcc
      601 tgttcgtctg tatgttgatt aatgtaatac acgaagatat catccaaatc tatctgagaa
      661 gaaatgttac gcagttcttc caaagtgcca tccgctaaga ttttcccttt atgaatcata
      721 acaattctat ctgctaaacg ttctgcctct cgcataatgt gagtggaaaa taaaacacat
      781 ttccctcgtt gcttacaatc ccgaataaac gaaacaatat ttctggcagt taaaatatct
      841 aaaccggcag tcggttcatc aaaaatcatt acaggcggat cgtgaataat ggaacgcact
      901 attgaaacct tctgcttcat acctgaagat agaaactcta tctttttgtc cagaaattca
      961 ttcatatcaa gtagatcagc catcactttt attcgttgct ctataagttg atcttcaatg
     1021 ctgtaaagat gtccaaaata agtgatgaac tctctgcaac ttaaacgatt atatagtcct
     1081 gtatctccag atagaaaacc aatattctct ctcaccttat ctggatgctt tctaatatcc
     1141 catccctgaa tttctgcaga gccttctgag ggctgaaaaa tagtggaaag aaccctcatt
     1201 gtggttgttt ttccagctcc atttacacct aaaagacaaa ctatttctcc atctcctgct
     1261 tcaaaattta tcttatttac ggcgtaaaaa ttcgttccgt ctttttttat aaagactttg
     1321 ctcaggttgt ctactttgat cataaatata ctcccaattt aagttagcat tcatttgtac
     1381 tttaattaac gatgcttttt cttaaaaact tacagacaca aaggactaat agcgacgata
     1441 attattttac ttatagccta ttacagcatc agaattctac gaagaagaat taaattcaat
     1501 ttccttctcc atatatatag agggtgacaa atatcttatc ttggctacaa aattccttaa
     1561 aacctatttt tgaagatatg aatttttgtc gaggttttta ttgttacaac agaggaatgt
     1621 gctattgatg gataaataag cataattccc attggatata gtctggctta gctgttttta
     1681 tgaattcaga aacataacta actctattca tttcatatct tatgatctgt aagagatttt
     1741 ttaccatcat ttatgtttat tctattagac tgtaattctt tttagcaagc aaagttcgaa
     1801 gatgctagcg attatttacc tacacaattt actactatac aaataaaact tgacctttca
     1861 gcaatcttat ttgaagtggt tataaaaata agggaatata ctatgaaaga aaaagcaact
     1921 ctcattttag ggggtggttc tgcactgggc ttagctcaca tcggggtttt aagtatttta
     1981 gaagaggaat ttgaaataac cggaattatc ggaagcagta tgggaagtat aattggtgga
     2041 ttatattctt ccggactaag ttccgctaag atatatgaac ttgcaggcag ttttaataat
     2101 gccaaattat tttctccctt caagttagat aaaaccatca aaggtatttt tgatggccgg
     2161 agtttattaa atttatttct aacctggact gagggtaaaa gcatagaaga aagtgaaatc
     2221 ccctttattg cttgtgctta tgatttaaca agtaagagca cagttctttt caataaagga
     2281 ctttatgcag atgcaatgag agcttcttct tcgttacctg tattatttgc tccttataaa
     2341 ctaaatcaat acatttttgt tgatggagga attgaacatc ccttacctat ggctttttcc
     2401 tctttgcttt ctgaagataa agtaatcgct gttaatgttt tacctgaagc atccagaagt
     2461 gccagtttca taaatctgga aagtgaagaa gaaactaaaa ccaagcgttt aagcagaact
     2521 gaagtaattt tcaatgctgt tttgcaaaat caagcatatc tggcaattcg agatatagca
     2581 atgttcagtc cagatatcgt aattgaagca ggtatgccta atggtcgtcc tttcgctttt
     2641 cataaagcac gagaatttta taattttgga cgccatcaag caatggaaac tttagcaaac
     2701 tataaagagc ccagatttat gcctactatt cgtaaacatt atagaaatct aattcagcat
     2761 ctacccaatc tgaacattcc tgttaaagat aatacttgac ggaaaactgg ctttttaaaa
     2821 taatagaaat tgaaaatatg agataatttg cagttatctg aactgctcaa tctgagaaga
     2881 ttataccaaa gaacactaat cccaaataaa taacaagcga ggagttcttt tatgaatgaa
     2941 atcatcaaaa tactgtctaa ggtggatggt ggtaatattt tagatgccgc aaccggaaag
     3001 ggagatttta tcaatacctt gaaacaacac ctaaaatctt ttacacaaat aattggagta
     3061 gatacctcgc aacgttgtgt ggattacgca caaaaaatat tccctgagaa taatatagaa
     3121 atataccgaa tgaacctgga agatatgcaa ttcgaagatg gttattttga tactgtttgt
     3181 atttctgatg cccttcacca tataaaaaac cgagaagcgg ttttaaacga aatgctaaga
     3241 gttttaaaac cagagggctt gatgctgatc acagaaatgt attctgatgg agaccaaaca
     3301 cctgctcagg aaactcatat tatgatgcat cattgggttg cttctgttga tcgccttttg
     3361 ggaacatacc atcaaaaaac ttttacccgc caagaactgc aagatatagt aaaaaaacta
     3421 cccctgaaaa aagtggagat cgcagatttc tatgtacctg tggatgatcc cagtaaaaat
     3481 tgtcctgcat tgatacgcaa ttgtcaagat attataaaac gcttgcagac aatggaaaac
     3541 acagaggcaa taattgcaga aggaaaagaa ttgatggaac ggattaaaga tattggatat
     3601 gccggtgcct gtcgattagc aattaccggt tataaaaaaa aaataagcta taattaatgg
     3661 ctgataaagg agataactaa tgctgtttga agaggatcta tttagcttga gtcaacttca
     3721 actatcccgc ttggcaaaaa aaatcggttt gcctggctat acacaatata aaggaaccga
     3781 actaatcttt aaactacttg aatttcaggc agctcaagaa ggtcttgcct tcgttactgg
     3841 gtgtctggaa attatggaag atggatttgg atttcttcgc tttcctgaca acaattataa
     3901 tgctggtcgc gatgatgttt atgtatcatt aactcagata agacgatttg ggcttaaaac
     3961 aggtaacatg attagtggac ctgttcgcag tcctaaagaa ggagaaaaat attatgccct
     4021 gcttagggtg gaagcggtta attatctgga accaaattca atgttgaatt tacggtcctt
     4081 tgatgaattg accccatatt atcctactga acgcttaaac ttagaatttg atccaaataa
     4141 ctttagcact cgcataatta atttattcac tccaattgga aaagggcagc gtgggttaat
     4201 tgttgcagct cccagaactg gaaaaacaac tttgttacaa gatacagcaa atgctatttt
     4261 gagcaatcat cctgaagttt atcttattgt tttacttgta gatgagcgcc ccgaagaagt
     4321 aactgaaatg aaaaaaatcc taaaacctgg taacagagaa gttatcagtt ctacttttga
     4381 tgaatcacca aaaaatcatt ctgccgtaag cgaaatggtt ttagaaaaag ccaaacggat
     4441 ggtggagctc aatcaggatg tagtaatcgt tttggatagt ataacgcgat tggcaagagc
     4501 ttataataat ataactcctt ccagcggaaa agttttaagc ggtggtattg atgccaatgg
     4561 cttaattaaa cccaaaaaat tctttggctc tgctcgtaac actgaagagg cgggtagttt
     4621 aactataatt gctactgctt tgattgatac aggcagcaaa atggaccagg taatttttga
     4681 agaatttaag ggaactggaa atatggaact tgttttagac cgctccatca gtgatatgag
     4741 attgtatcct gcaatagatt tagtaaaaag cggaacccga cgcgaagaat tacttctatc
     4801 tcaaaatgaa ctaaaccgaa tgtatgtgtt gcgtaaatat ctgaaaacga tctctcccat
     4861 tcaagggatt gaaaccttac gcaagaagat gcaggcaacc gataccaacg aagaacttct
     4921 aaattcaatg agcaattaat gattccaact cgctattcag ttattaagcg aatccttttt
     4981 tcctttatct tgattagttt atgcacagtt gtttactccg ctgttagaaa ggaatttcag
     5041 gaactgaata atttgtatag caagggaaag ctggatgagc tatctgaagc tataagtaat
     5101 ctgaaacctg ttaatgatga agaacgtgct tgcctgggat attataatgc aatgctaaaa
     5161 actaaaaccg aggataccat atctgctcat caatggctaa tcgaaagatt tcccaaaagt
     5221 ccttatgctc aaaaatccct tctggaatta ggtaaaatat atattctgga aaggaaaatt
     5281 gaggaagcca ctgtgtattt aagacggata aattccacag atttaattga a
//
LOCUS       DAORHB010000092         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_16011_length_5315_cov_5.358555, whole genome shotgun sequence.
ACCESSION   DAORHB010000092 DAORHB010000000
VERSION     DAORHB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16011_length_5315_cov_5.358555"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..582
                     /gene="rpmH"
                     /locus_tag="PKM80_03700"
     CDS             448..582
                     /gene="rpmH"
                     /locus_tag="PKM80_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNV93044.1"
                     /translation="MKRTYQPSNRSRKNTHGFRSRMATKNGRKVLARRRAKGRKTLTV
                     "
     gene            587..919
                     /gene="rnpA"
                     /locus_tag="PKM80_03705"
     CDS             587..919
                     /gene="rnpA"
                     /locus_tag="PKM80_03705"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNV93045.1"
                     /translation="MVRWITSHSEYVDFYNPDFQFRTAYFYVPVLYSKGETAVGITIG
                     RKIGNAVKRNLLKRRIKSWLYSNQEKLPIGFRFNLVARIGAADLQWLELSQELLKVIT
                     QINTKAVY"
     gene            853..1146
                     /gene="yidD"
                     /locus_tag="PKM80_03710"
     CDS             853..1146
                     /gene="yidD"
                     /locus_tag="PKM80_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNV93046.1"
                     /translation="MAGIIARAIKSNNSDKYKSGLLRQILRLPNLLFLFLIRFYQLAF
                     SPFLPPSCRFEPSCSVYGYQAFKKYNFFKAFYLTSMRILKCNPFHKGGYDPLP"
     gene            1157..2788
                     /gene="yidC"
                     /locus_tag="PKM80_03715"
     CDS             1157..2788
                     /gene="yidC"
                     /locus_tag="PKM80_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNV93047.1"
                     /translation="MEKRTLIALLLVFILFLVFNEFVWKPQQIRHQQQLQEQQAVTPE
                     PVKEQVKPDSSQLIPLADTLLAKGEHPEIIKLSNQYMTVTFNSRGGIIQQVELHNFVM
                     HDSTKVKLIPENASIASVKIIHPASETALQDVVFQYKISPDSKGVTFFLGEESQPKLI
                     KSFALDDQYGIALEVEVANYLVTNGIELDFGSGIADTEKTTKSKLQDYRFMFYADNEI
                     FKTTLAKMKKKQPSGTFNSFNWMALRGKYFTLVLKENEPALMRSYTSTLNPETGNPAF
                     VIDSRQSNPKRSWKQSFIIYAGPADYQILQKYGKQMDSIPERGASWLRWLSNIFAGLL
                     KFLHRYIKNYGVVILIFALIIKLLLHPLTQHQMVHSMRQQKMQPYIEEIRKKYANDVV
                     KQREELTKLYKENKTSMTAGCLPLLIQMPILIPLYSVLRYSLDMRNASFCLWLKDLSE
                     PDPYMILPIIMGGFMLLQSLMMRPRNVDTSQMTEQQKMQQSQMKIMTWGMPIFMIIIF
                     RNMPAGLVLYWTTFNVLSIIHQYFLTKHLKKKENQ"
     gene            2785..3573
                     /locus_tag="PKM80_03720"
     CDS             2785..3573
                     /locus_tag="PKM80_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HNV93048.1"
                     /translation="MTTIDKSGESLEKIIKDFRNEYKIHDWELQYEILKKPSKGIFGL
                     FAHKLALVRFQLPSSLDRAKLFIQNLLEKMRIKYTSLESRMEGKTIYLDINGSPDSGF
                     LIGKNGSMLDNLQYLLNRVFENDRQVERIYLDVDGYRERQADNFLKPYLSQIVNVKLS
                     DKPVTLEPMNAAERRIIHRFVEKENGLRTLTIGEGERKRIVIFSAKQSEKEVLSKSKK
                     YSQHRSKSTDPQTKNKSGKPKSTPGKGNPKPHIKQPQMSNPKHQ"
     gene            3570..4400
                     /locus_tag="PKM80_03725"
     CDS             3570..4400
                     /locus_tag="PKM80_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV93049.1"
                     /translation="MKQIYPVPAGFYWSDSGAFMGVLPYALWSKKSEIDERFRRKLNL
                     NLLLIQSGNRNILIDTGLGNRLSAKQREIYQPSEFLLPVSLAELGLQDKDITDVIMTH
                     LHFDHAGGIVTDFGTYEALTFPKAHYWIQKAEWEMAKNPDELNQAAYNYEQQLSLLEQ
                     KGNITLLEGEAEIEPGITLTLVGGHTVGSQIITINFGTEYFIYAGDIIPTMFHTSLAI
                     TSAYDVSRIQTVAAKKLIYSRLQEHNGILLLNHDLSHWQVPFSELKIGKKEKKDKMDS
                     "
     gene            4539..5045
                     /locus_tag="PKM80_03730"
     CDS             4539..5045
                     /locus_tag="PKM80_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93050.1"
                     /translation="MFFTNRIIKIVSFCLVGLLLSSCVKYRGIEFPVNPPPSFEKNYI
                     LLIWTQKGKSKTNPKKSTLQANELFMKNITIAGDTLTGFVYNKPNKKHIKNQISVYID
                     DSIAQPDSIPSPFSLPVSAIQKIETYEVNVGTTILTSILGIATTTLTIAIICVGYLII
                     YQIVTQYD"
BASE COUNT         1743 a         1003 c          984 g         1585 t
ORIGIN      
        1 gaaaatgata ataggcatag gatgaatagg tttcaaagga ttaaaattta gaaacaagga
       61 ttttaggatt ggattttcga aacaaggatt taaggattta aggattttag gattttagga
      121 ttaagaggat ttcgaattta aagttccgtt aggaacgata catactaacg acgggtttta
      181 acccggcgac aagaagaaca aattaaaaaa gtcccagcgg gacggcagat atggttttcg
      241 aaacaaggat ttaaggattt ggaggatata tcttccagaa aaaggtgtcc tccatctata
      301 taagttcggg gtcagcaaat gcctaaataa ctttcatccc tttctataaa tctggagtta
      361 attttaaaaa ttcttgacag agcaggcaca ggaaatttta tgacataata actctaaatg
      421 catacatctt taaggagata cataaaaatg aaacgaactt accaaccctc aaacagatcg
      481 cgaaaaaaca ctcacggttt tcgcagtaga atggctacca aaaatggtcg taaagtatta
      541 gctcgcagaa gagcaaaagg gagaaaaact ttaacagtat gagcttatgg ttaggtggat
      601 tacttcccat tctgaatatg tggattttta caatcctgat tttcagtttc ggacggcata
      661 tttttatgtg ccagtattat atagtaaggg tgaaacagct gtgggcatta ctattggtag
      721 aaaaatcggc aacgccgtta aacgcaattt acttaaacgc agaatcaaat cctggctgta
      781 ttccaatcag gaaaaattac ccattggttt ccgatttaat ttggtagcca gaattggagc
      841 agctgattta caatggctgg aattatcgca agagctatta aaagtaataa ctcagataaa
      901 tacaaaagcg gtttactaag gcagatattg cggttaccca atctgctttt tttgtttctc
      961 attcgttttt accagctggc attttctcct tttttacctc ccagttgccg atttgagccc
     1021 agttgtagtg tttacggata tcaagcgttt aagaaatata acttcttcaa agcgttttat
     1081 ttaacctcta tgcgtatcct gaaatgtaat cctttccaca agggtggata cgatccactt
     1141 ccctaaagga caagaaatgg aaaaaagaac tttaattgcc ttactccttg tgttcattct
     1201 ctttttagtt ttcaatgaat ttgtttggaa acctcaacag attaggcatc aacagcagtt
     1261 gcaagagcaa caagctgtta cccccgaacc agttaaagaa caggtaaaac ctgattcatc
     1321 acagcttata cctttggccg atacgctgtt agcaaaaggt gaacatcccg aaataataaa
     1381 actttccaat cagtatatga cggttacttt caacagtcgg ggtggcatta ttcagcaagt
     1441 tgagttgcat aattttgtta tgcacgatag caccaaggta aagttaattc ccgaaaatgc
     1501 ttccatagcc agtgtaaaga taattcatcc tgcttcggag acagctttac aggatgttgt
     1561 tttccagtat aagatttctc ctgattctaa gggagtaacc tttttcttgg gcgaggaaag
     1621 ccaacccaag cttattaaaa gtttcgctct ggacgatcaa tatggtattg ctttagaggt
     1681 ggaagtagct aattatttag ttaccaatgg catagaatta gattttggaa gcggtattgc
     1741 cgatactgaa aaaactacta aaagcaaact gcaggattat cggtttatgt tttatgccga
     1801 taatgagatt tttaaaacaa cattagcaaa aatgaagaaa aaacagccct ctggtacttt
     1861 caatagtttc aactggatgg ctttacgcgg taaatatttt actcttgtgt taaaagaaaa
     1921 cgaacctgct ctgatgcgtt cgtataccag cactttaaat cccgaaactg gcaatcctgc
     1981 ttttgtgata gattccagac aaagtaatcc caaacgaagc tggaagcaga gttttattat
     2041 atatgcaggc cctgcggact atcaaatctt gcaaaaatac gggaagcaaa tggatagcat
     2101 tccagaaaga ggtgcatcct ggttacgctg gctttccaat atttttgctg gtctactgaa
     2161 atttttgcat cgctacatca aaaattacgg agtggtaatt cttatctttg ctcttatcat
     2221 aaagttatta ctgcatccat taactcagca tcaaatggtg cacagtatgc gtcagcaaaa
     2281 aatgcagccc tatattgagg aaatccgtaa aaaatatgcc aatgatgtag taaaacaacg
     2341 cgaagaacta accaagctct ataaagaaaa taaaaccagt atgacagctg gttgcttgcc
     2401 tttactaatt caaatgccga ttttaattcc gctttacagt gttttgcgtt attctctgga
     2461 tatgcgaaac gcttcttttt gtttgtggct gaaagacctt tctgaacctg atccctatat
     2521 gattctgcct attattatgg gtggatttat gctcttacaa tctttgatga tgcgtccgcg
     2581 caatgtagat acttcgcaaa tgacggaaca gcaaaaaatg cagcagtccc aaatgaaaat
     2641 tatgacctgg ggtatgccga tttttatgat tatcatcttt cggaatatgc ctgcaggatt
     2701 ggttttatac tggactacct tcaatgtgct gtctataatt catcaatatt ttttaactaa
     2761 acacctcaag aaaaaggaaa atcaatgaca actatagata aaagcggcga atccctggaa
     2821 aaaataatca aggatttccg caatgaatat aagatccacg attgggagct gcaatacgaa
     2881 atcctgaaaa aaccctcaaa agggattttt gggttgtttg cccataagct tgctctcgtg
     2941 cgttttcaac taccttcttc tttggataga gctaagctct ttatccaaaa cttgctggaa
     3001 aaaatgagga ttaaatacac ctctttggag agcagaatgg aaggtaaaac aatttatttg
     3061 gacatcaatg gctcccccga ttctggcttt ttgattggga aaaacggctc tatgctggat
     3121 aatttgcaat atttattaaa tcgtgttttt gaaaatgacc gccaggtaga gagaatttat
     3181 ctggatgtag atggttatag agaacgccag gcagataatt ttctgaaacc ctatctttct
     3241 caaatcgtaa atgttaagct tagcgataaa cccgtaaccc tggaacctat gaatgctgca
     3301 gaacgcagaa ttattcatcg atttgtggaa aaggaaaatg gtttgcgcac ccttactatc
     3361 ggtgaaggag aaagaaaacg gatagtgatc ttctctgcca aacaaagtga aaaagaagtt
     3421 ctctccaaaa gcaaaaagta tagccaacat cgctctaaaa gcactgatcc acaaaccaaa
     3481 aataaaagcg gtaaacctaa atctactcca ggaaaaggaa acccgaaacc ccatataaaa
     3541 caacctcaaa tgagtaaccc aaaacaccaa tgaaacaaat ctatcctgtt cctgcaggtt
     3601 tttattggtc tgatagcggt gcctttatgg gtgttttgcc ctatgcgctt tggagcaaaa
     3661 aatccgaaat cgatgaacgc tttcgccgaa aactgaattt aaacctactc ttgattcaaa
     3721 gcggaaaccg caatatttta atcgatacgg gtttgggaaa tcgcctttcc gctaaacaaa
     3781 gagagatcta tcaaccaagt gaatttctat tgcctgtttc gcttgcggaa ttgggtctgc
     3841 aagataaaga tataacagat gtaattatga ctcatctgca ttttgatcac gccggcggaa
     3901 ttgttaccga ttttggaact tatgaagcat taactttccc caaagcacac tactggattc
     3961 aaaaagcgga atgggaaatg gctaaaaatc ccgatgaact aaaccaggca gcttataact
     4021 atgaacagca attatcgctt ctggaacaaa aggggaatat tactctcctt gaaggtgaag
     4081 cagaaattga accggggatt actttaaccc tggttggagg ccacactgtc ggtagccaaa
     4141 ttattactat taactttggt acggagtatt ttatatatgc tggagatatt attcctacta
     4201 tgtttcatac ttctttggcg ataacctccg cttatgatgt ttcccgcatt caaaccgttg
     4261 ctgccaaaaa acttatctat agtcggttac aggaacataa cggaatttta ctcctaaacc
     4321 acgacctctc tcattggcaa gttccctttt cggaattgaa aattggcaag aaagaaaaaa
     4381 aggataaaat ggatagttag ttggaacaat aaaggaagtg agaaattacc atactttggt
     4441 ttacatagct gccaataatc taaccaaaac cattgtcttt ccgttctttt cctacttttt
     4501 ccagctgtgg aagctgcaga aaaaagagag gagataaaat gttctttaca aaccgcatta
     4561 ttaagattgt atctttttgc ctggtaggat tattactttc ttcctgcgta aaatataggg
     4621 gtatcgaatt tccagtaaat cctcctccct cttttgaaaa aaactatatc ttgctgatct
     4681 ggacacaaaa aggcaaatcc aaaacaaatc ctaaaaagag tactttacaa gccaacgaac
     4741 tctttatgaa aaatataact attgcaggag acactttaac gggatttgtg tataacaaac
     4801 ctaataaaaa gcatataaag aaccagataa gtgtttatat tgatgattca attgcccaac
     4861 cagattcgat tcccagccca ttttctttac ctgtttctgc catccaaaaa atagaaactt
     4921 atgaagtgaa tgtgggaaca actattctta ccagtatcct tggtattgct acgacaactt
     4981 taaccatagc aatcatttgt gttgggtatt taataatcta tcaaattgtc actcaatacg
     5041 attaatatag agagtatagc agagggcttg cgctaccatc gctatcactg tgtcggcctt
     5101 tgagttaaaa tcggctttct atgtgatctg ccacctcatt tcattctttt ccactatctt
     5161 taactatggt cattaataat ctcatagaaa aggccgattt tgacttaaag ccgactaatc
     5221 gtcatttata aaatacaatc ctcttaatcc taaaatcctt aaatccttgt ttctaaattt
     5281 taatcctttg aatcctattc atcctatgcc tatta
//
LOCUS       DAORHB010000093         5276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_16204_length_5276_cov_5.011109, whole genome shotgun sequence.
ACCESSION   DAORHB010000093 DAORHB010000000
VERSION     DAORHB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16204_length_5276_cov_5.011109"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            668..2566
                     /gene="gyrB"
                     /locus_tag="PKM80_03735"
     CDS             668..2566
                     /gene="gyrB"
                     /locus_tag="PKM80_03735"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNV93051.1"
                     /translation="MQSSTYTASNIKVLKGLEAVRKRPAMYIGGTGERGLHHLVYEVI
                     DNSIDEAMAGFCDEIICTITNEGHIRVEDNGRGIPVDIQKDLGVPAVQVVLTVLHAGG
                     KFDDKSYKVSGGLHGVGVSVVNALSEFLEVNIHKEGKEYYMRFERGKPVTELQILGDS
                     KKSGTIVTFHPDPLIFETTEFSFDYLTTRLRELAFLNCGVRIILKDERSGRLHDFKYD
                     GGIKSFVEYLNQNKKPLTPNTIYLSNVKEGMEFEVALQYNEGYQENIFSFANNINTTE
                     GGTHLSGFKSGLTRAVNTYIKNADLLKNEKVSPSGEDIREGLTAVISIKIANPQFEGQ
                     TKTKLQNSEVEGIVNSAVYEKLLEYFEEHPAEAKNITMKSILAARSREAARKARELTR
                     RKSVLESGSLPGKLADCTLSDPAKTELFLVEGDSAGGSAKQGRDRSFQAILPLWGKML
                     NTEKARVDKVLNNDKIQPIILALGAGIGQDFDVSKLRYHKVIIMADADVDGSHIATLL
                     MTFFYRYMRPVIEEGKLYIAMPPLFLVRKGKQKVYVYNEEERDAAIKEFGEKGVIVQR
                     YKGLGEMNAEQLWETTLDPENRKMIAIKMDDAIEADRMFTILMGDEVEPRREFIQTNA
                     RYVKNLDI"
     gene            2580..5204
                     /gene="gyrA"
                     /locus_tag="PKM80_03740"
     CDS             2580..5204
                     /gene="gyrA"
                     /locus_tag="PKM80_03740"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNV93052.1"
                     /translation="MADTTNIIPVQIEEQLKQAYLDYSMSVIVARALPDIRDGLKPSQ
                     RRIIYAMHELNLTPGGHFRKCAKIAGDTSGNYHPHGEQVVYPTLVRLAQPWIMRYPLI
                     DGQGNFGSIDGDPPAAMRYTEARLQKITMEMLSELEKDTVEFRSNYDETRQEPTVFPS
                     RFPNLLVNGSSGIAVGMATNMPPHNIGEVCEAIIALIDNPDLQPLDLLQYIKGPDFPT
                     GGFILGTEGIKDYFETGRGRIIMRGEAEIDTLPNDSEQIIIRSIPYQLSTTQLIDRMV
                     ELVKDKRIEGISDIRDESGRDGMRLVIQVKRNHDAQVVRNLLYKHTQLQATFGVINLC
                     LIDGVPQIVNMKEMLQNFIEFRHDVILRRTLFDLRNAKDRLHILEGFKIALDNLDAVI
                     ATIRASQTPADASSALQEKFGLTEIQAKAILDMRLQRLTGLERDKIEIEYQELLITIA
                     RLTELVEHKELRMNLIKEETSEIQDKFADKRLTTIIPAPAGSFNMEDLIADEMVVVTI
                     THDGYVKRLPVDTYKVQGRGGKGLSASNLKEEDFIQYIFVASTHSYILLFTDHGMCYW
                     LKVFEIPEASRTARGKAIVNLVNLAEGDRIKAFVTLKDFHPEQNIIMCTRKGLVKKTP
                     LSAFSHPRSNGIKAIKLREEDELIDARISESGDDILLATHEEKCNRFSESEIRLVRRF
                     SQGVRGIRLRNGDYVISMTIATAQDQLENGNPNAATILAISENGYGKRSPISSYSCTR
                     RGSMGVITLKTNKRNGYLASLMLTYDDDDLMIITQGGMIIRQKVSEISVVGRNTLGVK
                     LINLAPNDVVRDISLIHSEPDEESLDKEVETFKAKAPFKFEEKAATDEEDYEEEDEEM
                     DVIDELEEEDLDNEEDNE"
BASE COUNT         1786 a          812 c         1084 g         1594 t
ORIGIN      
        1 attattatac aggagaaccc atccatattc ctaagccatc tgaaatacgt aagcaaaaga
       61 atataattgt agctaaatat aagcattaag ttaatgtata acattacatt atgatagtta
      121 gttcattaat agataaaatg ataatagata tttatccacc aagcctaagc cgtagctaaa
      181 gtttttttag ctcatgggtt gctgattatc gaccttttaa ataaccttga gaattggttt
      241 cgtagcatta atatcttata acggatagga atttactacc ccaccatatc ccatttgttt
      301 atattaagtt aaccttgatt tatctttact acacagcgtt atgtagacga taattatccg
      361 atatctatcc cttatgcgat aagggaatca taagggaagt ataagggaag tataagggaa
      421 gctatatggc aagataaaag ggggaaaaag gggtgagttt tagggcttag aaaacagtaa
      481 gaataatgga tttataactt attatataac aagaggttgt aaatcataaa taatataaga
      541 aagtacttga caataaactc atatcatata ctgtggcaac aaattaagga tatattactt
      601 agagcttaac gcttttctgc taaccaatcc ttattattat ttgtaaaact ataggagaca
      661 atatattatg caaagttcaa cttatacagc cagcaatatt aaagtgctga aaggtctgga
      721 agcagttcga aaacgccctg caatgtatat tggaggaacc ggtgaacgag gattgcatca
      781 tcttgtatat gaggtaattg ataattccat agatgaagca atggcaggtt tctgcgatga
      841 aattatttgc acaattacga acgaaggaca tattagagta gaagataatg ggcgtggaat
      901 tcctgttgat attcaaaaag atttaggagt tccagccgtg caagtagttt taacagtttt
      961 gcatgcgggt ggtaaattcg atgataagag ctacaaagtt tctggaggtt tacatggtgt
     1021 aggtgtatcg gtagtgaatg ccctttcaga atttttggaa gttaatattc ataaagaagg
     1081 caaagaatac tatatgcgtt ttgaacgggg taaacctgta acggaattac aaatattagg
     1141 agacagcaaa aagtccggaa caatagtaac cttccatcct gatccactta tttttgaaac
     1201 tacagaattc agttttgatt atcttaccac tcgcttaaga gagcttgctt tcttgaattg
     1261 tggagtgcgt attattttaa aagatgagcg aagtggtcgt cttcacgatt ttaagtatga
     1321 tggtggaata aaaagttttg tggaatattt gaatcagaac aaaaaacctc ttacccccaa
     1381 tacgatatac ctctccaatg tgaaagaggg tatggaattt gaagtagcgc tacaatataa
     1441 tgaaggttac caagaaaata tcttcagttt tgccaataat atcaacacta ctgaaggtgg
     1501 aactcattta agcggattta aaagtggtct tactcgcgca gttaatactt atataaaaaa
     1561 tgctgatttg cttaagaatg aaaaggttag tccatctgga gaagatattc gagaaggcct
     1621 tacggcagta atttcaatta agattgccaa tcctcagttt gagggacaaa ctaaaaccaa
     1681 gctccaaaat tctgaagtag aaggaattgt aaatagcgca gtctatgaaa aacttttgga
     1741 atattttgaa gaacatcctg ctgaagcaaa aaatattaca atgaaaagta tattagctgc
     1801 acgcagtaga gaagccgctc gtaaggcacg tgaattaacc aggcgtaaat ctgttttaga
     1861 aagtggttct ctgcctggaa aacttgcaga ttgtacttta agcgatccag caaagacaga
     1921 actatttttg gttgaaggtg attctgcagg tggaagtgca aaacaaggta gagatcgttc
     1981 ttttcaagct attcttcctt tatggggaaa aatgctaaat acggaaaaag caagagtaga
     2041 taaagttttg aataatgaca aaattcagcc gattatttta gctttgggag cgggaattgg
     2101 acaggatttt gatgtgagta aacttcgtta tcataaagtc attataatgg ccgatgctga
     2161 tgtagatggc tctcatattg ccactttact tatgaccttc ttctatcgct atatgcggcc
     2221 tgtaattgaa gaaggtaaac tttatattgc aatgccccct ctgtttttgg ttcgtaaagg
     2281 gaaacagaaa gtgtatgttt ataacgaaga agaacgagat gctgccataa aggaattcgg
     2341 agagaaaggt gtgattgttc aacgctataa aggattaggt gaaatgaatg cagaacagct
     2401 ttgggaaact actctcgatc cggaaaaccg caagatgatt gctatcaaaa tggatgatgc
     2461 tattgaagct gatagaatgt ttactatttt aatgggcgat gaagttgaac ctcgtaggga
     2521 atttattcaa acaaacgccc gttatgtaaa aaatctcgat atttaaggag ttaaaataga
     2581 tggcagatac aacaaatatt attccagttc aaatcgaaga acaactgaaa caggcatatt
     2641 tagattattc tatgagcgtt attgttgcac gcgctttacc tgatattcgc gatggtttaa
     2701 aaccctctca acgcagaatt atctatgcga tgcatgaact taatttaaca cccggaggcc
     2761 actttcgtaa atgtgctaaa atagctggtg acacaagcgg taattatcat cctcacggag
     2821 aacaagttgt atatcctact ttagtgcgtt tggctcagcc ttggataatg cgttatcctt
     2881 taattgatgg acaaggaaac tttggttcta ttgatggaga tccacctgca gcaatgcgtt
     2941 atacagaagc ccgtttacaa aaaataacaa tggaaatgct ttctgaactg gaaaaggata
     3001 cagttgaatt tcgcagtaat tatgatgaaa ccagacaaga gccaacagtt tttccttccc
     3061 ggtttcctaa tttattagta aatggttctt caggaattgc tgttggtatg gcaacaaata
     3121 tgccacctca taatattgga gaagtttgcg aggcaattat tgctttaata gataatcctg
     3181 atttgcaacc gctggatttg cttcaataca taaaaggtcc agatttccct acaggtggtt
     3241 tcattttagg aacagaaggt attaaagatt attttgaaac aggtagagga agaattatta
     3301 tgcgcgggga agcagaaatt gatactttac ctaatgattc agaacagatt attatccgtt
     3361 ctatacctta tcaattaagt actacacagc tcattgatcg tatggttgaa ttagtaaaag
     3421 ataaaagaat tgaaggcata agtgatatcc gtgatgaatc tggtcgggat ggaatgcgct
     3481 tagttatcca agtaaaacgg aatcatgatg ctcaagttgt tcgtaattta ctatataaac
     3541 atacgcaatt acaggcaact tttggcgtta ttaatctctg tcttattgat ggtgtacctc
     3601 aaatagtaaa tatgaaagaa atgttacaaa atttcatcga attccgtcat gatgtgattt
     3661 tgcggagaac actatttgac ctgcgtaatg ctaaagatcg tttgcatatt ttagaaggat
     3721 ttaaaatagc gctggataat ctggatgcag tgatagctac tattagagct tcacaaactc
     3781 ctgcagatgc aagttctgct cttcaagaaa aatttgggct cactgaaatt caagcaaaag
     3841 caatattaga tatgcgtttg caacggttaa caggtttgga aagagataaa atcgaaatag
     3901 aatatcaaga attactgatt actattgccc gacttactga actggttgaa cataaagagc
     3961 tacgaatgaa cttaattaaa gaagaaacga gcgaaataca agataaattt gcagataagc
     4021 gtttaactac tattattcca gctcctgcgg gtagcttcaa tatggaagat ttaattgctg
     4081 atgaaatggt tgtagttact atcactcatg atggttatgt aaaacgttta cctgttgata
     4141 cttataaggt tcagggaaga ggaggaaagg gcttaagcgc ttctaatcta aaagaagagg
     4201 attttattca atacatcttc gtagccagta cccattccta tatattactc tttacagatc
     4261 atgggatgtg ttattggcta aaggtttttg aaatacctga agctagtaga actgccagag
     4321 gtaaggcaat cgtgaattta gtaaatcttg ccgaaggtga tagaattaaa gcgtttgtaa
     4381 ctcttaagga ttttcatcct gaacaaaata ttattatgtg cacccgtaag ggactggtta
     4441 aaaaaactcc tctatctgct ttttcgcatc cacgttctaa tggtattaaa gcaatcaaat
     4501 taagagaaga ggatgaattg attgatgcca gaattagtga atctggtgat gatatccttt
     4561 tggctactca tgaagaaaag tgcaatcgat tcagtgaaag tgaaattcgg cttgtgcgtc
     4621 gtttttcaca aggtgtgcgt ggaattcgtt tgcgtaatgg tgattatgtt atttctatga
     4681 caattgccac tgctcaagat caacttgaaa acggtaatcc aaatgctgct actattttag
     4741 ccatcagcga aaacggttat ggtaagcgta gtccaatatc cagctattcc tgtacccgtc
     4801 gtggttctat gggagttata actctaaaaa ccaataaacg aaatggctat ttagcatcat
     4861 taatgctgac atatgatgat gacgatttga tgattattac tcaaggtgga atgattattc
     4921 gccaaaaggt ttctgaaatt tctgtagtag ggcgtaatac tttgggcgta aaattaatca
     4981 atcttgctcc caatgatgtt gttcgtgata tttctttaat tcattccgaa ccggatgaag
     5041 aatcacttga taaagaagtg gaaactttca aagcaaaagc tcccttcaaa tttgaagaga
     5101 aagctgctac ggacgaggaa gattatgaag aagaagatga agaaatggat gtgattgacg
     5161 aattagaaga agaagacctt gataatgaag aggataacga atagactaat aaaaatgata
     5221 ttaccggcag taattttatt actgccggta gcttgttctg taaatcaatt tgttcc
//
LOCUS       DAORHB010000094         5254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_16303_length_5254_cov_5.639931, whole genome shotgun sequence.
ACCESSION   DAORHB010000094 DAORHB010000000
VERSION     DAORHB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16303_length_5254_cov_5.639931"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKM80_03745"
     CDS             <1..251
                     /locus_tag="PKM80_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93053.1"
                     /translation="YPQYELAKLEEVKDLPTFEIYDAPQIAGIRSKPKRAITVIVSTV
                     AAFIFSCILALILESFQGENREKILAIKAALFRKRKQS"
     gene            641..1522
                     /locus_tag="PKM80_03750"
     CDS             641..1522
                     /locus_tag="PKM80_03750"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNV93054.1"
                     /translation="MLAYLGIAFWEISTWKHLPQSIYYNRAIFVPTGPFYGPNQFGAV
                     FCLLFPFYLFIPELTKNRRYLRVFSFICALLVIAIVIVEGARIAIIATLGMLLYTLFF
                     LYSKKMRRTGLLLIGLIILGLIIFAKPLVNFTISKLSAEISSIGQESKSCVMGSIKIR
                     TRLIPENLEILAQSGFMGVGAGNVERYMLSGRIYRTGGISNPHNFFLELMANYGLVFL
                     LGFLYIYGIWLYRLWRAIKANPEQKKYYEMFFFVLLMFIPAASLPSSIIWEHHYWILF
                     AFINEISHPANWMQKAI"
     gene            1519..2286
                     /gene="mazG"
                     /locus_tag="PKM80_03755"
     CDS             1519..2286
                     /gene="mazG"
                     /locus_tag="PKM80_03755"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNV93055.1"
                     /translation="MSKFEKLVEIVATLRTPGTGCPWDLKQTRETLVPNIIEELYEVI
                     EAIENKDDNSLKEELGDLLLHIVFQAQISKEQGLWSIDDVIDEIINKLIRRHPHVFGD
                     LNIEDADAVKVNWERLKKKEKTMRKSVLDGIPKKMPALIQAQRTQEKAAAVGFDWQDI
                     KPIMEKIDEEKEEFIEALASNEEEKIREELGDMIFTLVNLARKLHIDAEAALKECTRK
                     FVRRFNSIEEYYRKEGKDINEADLEELDSHWQRIKGH"
     gene            2231..3784
                     /locus_tag="PKM80_03760"
     CDS             2231..3784
                     /locus_tag="PKM80_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV93056.1"
                     /translation="MKPILKSSILTGKESRDTNLFNRLRFILIFGSLAIFIFFGIYIQ
                     FLIKQAKQEQEYIPRIFAQYIAYTDKYLREAELYAQLITEISSKYLQFTTSSDFKQDL
                     WDYISTEFMQDNPVPVIITDQNFQPQLWKNVGVPTDTLFHELSAANQRKLENLMKTMI
                     QTPLTDNGKISGYAFYSKPISFAEFIKNVDCAIIVTDHYKQPLFWRNVGIPEAANYYE
                     ISTEEQQKLAHQIQEMEEIPLSNQADSLGYIYFSNPKSLSYIRYIVILELVFALTVVF
                     FGVYGLFLLSRTEKDTLWIGLAKETAHQFGTPITSLMGWLDYLKEPPVDGSERDINQI
                     VQYMTADLEHLKTIASRFGKVGSVTKLERNNLHQVIAETVEYFRARMPHLGNKIDIHY
                     ISKIDDETVLMDKELIKWTLENLIKNCMDAMITKGGNIIITATKKDPWIYIHIRDEGK
                     GIPRRQWKRIFEPGVTSKTRGWGLGLSLVKRIIEDYHNGSIKVLQSTIGEGTTFEIKL
                     PVQGINKEK"
     gene            3786..>5254
                     /locus_tag="PKM80_03765"
     CDS             3786..>5254
                     /locus_tag="PKM80_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNV93057.1"
                     /translation="MSYIFTPSQMKTLDENTINCFGLPARILMENAGKGCADYLLQNC
                     SEHLNNVIIILHGTGNNSGDGFVLARWLTIYGRRVLLCKVQDGTMTEECRLNYELCQS
                     LNIPMIGLDNISDTLEILDNSSYIGMIVDAVFGTGYKGKLPTSLKKIFQSANNSPAFK
                     VAIDIPSGLNGATGEGEDAFEADLTLCLHSPKIGSLLHTGKLKSGDLVTIPIGIPDSY
                     NDAFFSPALHIDAETFQVPKRYLLANKSDYGRVFIIGGSMGLLGSVAMSAKAALRAGA
                     GLVYLVSREEYAYFYNTNPSEVLFVGIPSKPESLLPDLSEFSSLIKKADSIVIGPGLG
                     TDEYGYIMLEFILRTSTVPTVVDADALRLIAKEPSLQQYIKKPNILLTPHFGEFCDLA
                     KIEMSALYKDTISELTKYVKNTEAKVLLKSDTTIFYDGKRMLINTTGNDGLATGGSGD
                     VLTGIIGSFCGQDMELGEAAINASFFMGFTTEYLATKRYP"
BASE COUNT         1671 a          880 c         1053 g         1650 t
ORIGIN      
        1 tatatccgca atatgaactt gctaagctgg aagaagtgaa agaccttcct accttcgaaa
       61 tatacgatgc acctcaaatt gctggtatta ggtctaaacc caaaagagca atcactgtaa
      121 ttgttagcac tgtggctgcc tttatttttt cctgtatttt ggccttgatt ttggaaagct
      181 ttcagggtga aaatagagaa aaaatattgg caattaaagc agctctcttt agaaaaagga
      241 aacaatcctg aattccgcaa ccctgaattt atcgtataat aacatcagat taaactatct
      301 gatagcggga ttattattga tagcggtagt tctggattac tttcctgtgg atatgggctt
      361 taatgatatc tattttatcg cggtggccag cctatgctta atttatgcta taatagataa
      421 ggatatcgca ataatcaatg aagttaatct tgttccggta ttgtttttat tagcattatc
      481 ggcttattca ctaataagtt ataactggtc gatagataaa aggatttcgt tagcccattc
      541 aaaattaatc ttaagaaaaa ccgcattatt tctcataata agtgtttttt gcagagataa
      601 aagagtgaag caatacgctc actggttctg gttttttcta atgctagcct atcttggaat
      661 tgctttttgg gaaatatcta cctggaaaca cttacctcaa tcaatctatt ataacagggc
      721 gatttttgtt ccaacgggac ctttctatgg tccaaaccaa tttggagctg ttttctgttt
      781 gttatttccg ttttatcttt ttattcctga gcttacaaaa aatagaaggt acttaagggt
      841 attttcgttt atatgtgctt tgttggtcat tgccattgtt attgtggaag gagccagaat
      901 tgccattatt gctacattgg ggatgttgtt atatacgctg ttttttcttt attcgaaaaa
      961 aatgagaaga acgggtcttt tactaattgg ccttattatc ctggggctca ttatatttgc
     1021 caagcctctt gtaaatttta caattagtaa attgagcgct gaaattagca gtattgggca
     1081 agaatcaaaa tcttgtgtta tgggcagtat aaaaattcgc actcgcttaa ttcctgagaa
     1141 tctggaaata ttggctcaaa gtggatttat gggtgtgggt gcaggtaatg tggaacgcta
     1201 tatgctttcg ggaaggatct atagaacagg tggaatttcc aatccacaca atttctttct
     1261 ggaactgatg gcgaattatg ggcttgtatt tcttttaggc tttctctata tttatggaat
     1321 ctggctctac cgtctctggc gagctataaa agcgaatcca gaacaaaaga aatattacga
     1381 aatgtttttc tttgtgcttt tgatgtttat acctgctgcc agcttaccca gctcgataat
     1441 ttgggaacat cattactgga ttctatttgc gtttatcaat gaaatatctc atccagcaaa
     1501 ctggatgcaa aaggcaatat gagtaaattt gaaaaacttg ttgaaattgt agcaactctc
     1561 agaactcctg gaacaggatg tccctgggat ttaaaacaaa cccgcgaaac tcttgttccc
     1621 aatattattg aggaactata tgaagttatc gaagccatag aaaataagga cgataactcg
     1681 ctgaaagaag aattgggcga tcttctattg catatcgttt ttcaagcaca aatatctaaa
     1741 gaacagggat tatggtctat agacgatgtg atagatgaaa ttatcaataa actcattcgt
     1801 agacatccac atgtttttgg agacctaaac atagaagatg cagatgcagt aaaagtaaat
     1861 tgggaacgcc ttaaaaagaa agaaaaaaca atgcggaaaa gtgttttgga tggtattcct
     1921 aaaaaaatgc cggctcttat tcaagcacaa cgaacccaag aaaaagctgc cgctgtaggt
     1981 tttgactggc aagatattaa gcccattatg gaaaaaatag atgaagagaa agaggaattt
     2041 atagaagcgt tagccagtaa cgaagaggag aaaatacgcg aagaacttgg agatatgatt
     2101 tttactttgg taaacttggc aagaaaatta catatcgatg ccgaagcggc tttaaaagaa
     2161 tgcacgcgta aatttgttcg ccgttttaat agtattgaag aatactaccg taaagaagga
     2221 aaggatatta atgaagccga tcttgaagag ctcgattctc actggcaaag aatcaaggga
     2281 cactaatcta ttcaatcgcc ttaggtttat tctaatcttt ggcagtttag ccatctttat
     2341 ctttttcggg atttatatcc aatttttgat aaaacaggca aaacaggaac aagaatatat
     2401 accccgcatc tttgctcaat atattgctta cacagataag tatttaagag aagcagaact
     2461 atatgctcaa ttaattacgg aaataagctc caaatattta caatttacta cttcgagtga
     2521 ttttaaacaa gacctgtggg attatatcag cacagagttt atgcaagata atccagtccc
     2581 agttattatt accgatcaga attttcagcc acaactctgg aaaaatgtag gagtgccaac
     2641 tgatactttg tttcatgaac tctcggcagc gaatcaacgg aaattggaaa atttgatgaa
     2701 aacaatgatt caaaccccct taacggataa tggcaaaatc agtggctatg ccttttacag
     2761 taaacctatc tcttttgcag aatttataaa aaatgtggat tgtgctatta ttgttaccga
     2821 ccattataaa caacctcttt tctggaggaa tgtaggtatt cctgaagctg ctaattacta
     2881 tgaaatctct actgaagaac aacaaaaatt ggctcaccag attcaggaaa tggaagaaat
     2941 acctctttcc aatcaggcag acagtttggg ctatatttat ttttcgaatc ctaaatccct
     3001 ctcctatata cgctatatag ttattttgga gcttgttttt gctctaactg ttgttttctt
     3061 tggagtatat ggcttatttc tacttagcag aacagaaaag gatacgcttt ggataggctt
     3121 ggcaaaagaa acagctcatc aattcggaac tccaataacc tcattaatgg gttggttgga
     3181 ttatttaaaa gaaccacctg tagatggcag tgaaagagat ataaatcaaa ttgtgcaata
     3241 tatgactgcc gatttagaac atctaaaaac tattgcttct cgctttggga aagttggcag
     3301 cgttacaaaa ctggaacgaa ataatttaca tcaagttatt gctgaaacag tagagtattt
     3361 tcgggctcgt atgcctcacc tgggaaacaa aatagatatc cattacatca gcaagattga
     3421 tgatgaaaca gttttgatgg ataaagaact aattaaatgg acattagaaa acttgattaa
     3481 gaactgtatg gatgcaatga ttactaaagg tggaaacata ataattacag ctaccaagaa
     3541 agacccctgg atctatattc atattcgcga tgaaggtaaa ggaattccac gcagacaatg
     3601 gaaacgaatt tttgaaccag gggtaacttc caaaaccaga ggttggggtt tggggctcag
     3661 cttagtaaaa cgcattattg aagattatca taacggcagc ataaaagttc tgcaaagcac
     3721 tattggtgaa ggcaccactt tcgaaattaa gcttcctgtt cagggaatca ataaggagaa
     3781 ataaaatgag ctacattttt accccttcac aaatgaaaac ccttgatgaa aatacgatta
     3841 attgttttgg tttgccagct agaatcctga tggaaaacgc aggtaaaggt tgtgcagatt
     3901 atctgctgca gaactgttcg gaacatttga ataatgtgat tattatttta catggaacag
     3961 gaaacaacag cggagatggc tttgttcttg cccggtggct aactatctat ggtagaagag
     4021 tccttttatg taaagttcag gatggcacga tgacagagga atgtcgtctc aattatgaac
     4081 tttgccaatc gcttaacatt ccaatgattg gattagataa tatttctgat actctggaga
     4141 ttctggataa cagctcttat attggtatga ttgttgatgc tgtgttcgga actggctata
     4201 aaggtaaatt acctacttcg ctaaagaaga tctttcagag tgcaaacaac tcacctgcat
     4261 ttaaagtagc aatagatatt ccttctggat taaatggagc taccggagaa ggagaggatg
     4321 cttttgaagc ggacttaact ctttgtttac attcgcctaa aattggctct ttgctacaca
     4381 caggaaaatt gaaaagcgga gatcttgtta caattccgat tgggattccg gatagttata
     4441 atgatgcttt tttctctccc gctcttcata ttgatgctga aacatttcaa gttcccaagc
     4501 gttatttgtt ggctaataaa tcagattatg gtagagtttt tataataggc ggttctatgg
     4561 gacttttagg ttcggtggca atgtctgcca aggctgcttt aagagcggga gctggtttgg
     4621 tttatttagt cagtcgcgaa gaatatgcct atttctataa taccaatcct tccgaagtat
     4681 tgtttgttgg tattccgtct aaacctgaat ctttgctccc tgatttgagt gaattttctt
     4741 ctctgataaa gaaagctgat agcatcgtta ttggaccagg tttaggaacg gatgaatatg
     4801 ggtatattat gttggagttt atattaagaa cttccacagt tcccacggtt gtggatgctg
     4861 atgctttacg actaattgcc aaagagccat ctttacaaca gtatattaaa aaacccaata
     4921 ttctgcttac tcctcatttt ggagaatttt gtgacctggc taagatagaa atgagtgctc
     4981 tctataaaga taccatctct gagctcacta agtatgttaa aaatacagag gcaaaagttc
     5041 tcttgaaaag tgatactacc atcttctacg atgggaaaag aatgttgatt aataccacag
     5101 gtaatgatgg acttgctaca ggtggaagcg gagatgttct tactggcata attggttcct
     5161 tttgcggaca ggatatggaa ttgggagagg cagctataaa tgcttccttt tttatgggtt
     5221 ttacaacgga atatcttgct acaaaacgct atcc
//
LOCUS       DAORHB010000095         5183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_16666_length_5183_cov_5.141966, whole genome shotgun sequence.
ACCESSION   DAORHB010000095 DAORHB010000000
VERSION     DAORHB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16666_length_5183_cov_5.141966"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKM80_03770"
     CDS             <1..377
                     /locus_tag="PKM80_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV93058.1"
                     /translation="IPLKDIDPVFDYYTPVIISSEDFLAKNPETAQKFMSALKKGYEY
                     CISKPDSAADILLKNVPELNAEQVRRSMQYLAKEFQSDAPTWGRQKPIVWKRFCDWMY
                     QQRLIQLAVEPNKAFTNDYLPK"
     gene            382..1149
                     /locus_tag="PKM80_03775"
     CDS             382..1149
                     /locus_tag="PKM80_03775"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV93059.1"
                     /translation="MSDCILEAQDIRKSFFFKGSLITVLDNVQFHIRTGQFVSIIGPS
                     GCGKSTFLELLAGITTPDEGFILLKGNDITAKTGFLGYMPQDDLLFPWLTVMKNILLP
                     VKVTGSNEQEAKQKINELLPLFGLQNYTEHLPYQLSGGLKQRVALLRTYMCSADILLL
                     DEPLAKLDALTRSQLQSWLKDIVHLLNLTVILVTHDIDEAIMLSDRIDLMGINPGTFI
                     ESIKLDRNNPLTAEEQLKLKIKLRSQLVNGESNNDKK"
     gene            complement(1759..2997)
                     /locus_tag="PKM80_03780"
     CDS             complement(1759..2997)
                     /locus_tag="PKM80_03780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV93060.1"
                     /translation="MNWGKDILFSLVLFLLASFCLLSILGINSEPLPELKLDISKPTE
                     PWIIEIIPPGGSIFSVLANQNVPPQEIGLLAYNFGDYIDVTTIQPGDTLKVLLSEDKQ
                     HIKKLMFVQEPTTRHHFTVSGDSLIYHLEALPVKKAKRIIEGTIQETLDATLLTMGFA
                     PQDKQAINNGLETEINFSHDARNGDQFLVFVEERIFEGKTLPGRKIFYVQYTGERTGT
                     CELFRYEDAEENSVLNGLYTIEGKSYHSNGVGFPLSVIHVVSSFGKRLDPFHGSWANH
                     QGVDYRAHYGTPVYAVAGGKVTTASYNGGWGNEIRINHPSGLSTQYAHLSSINVHKGQ
                     NVKKGQIIGRVGSTGRSTGAHLHFGLIKGKKYINPTNLKMVGTEKLNETQMARFKSQQ
                     QTILQEMQNLKHPGPLITKK"
     gene            complement(2994..3938)
                     /locus_tag="PKM80_03785"
     CDS             complement(2994..3938)
                     /locus_tag="PKM80_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNV93061.1"
                     /translation="MADRDSITRVTVNLGLFSNILLSIIKTCVGIFGHSAALLADGIN
                     STSDVVYYIAVKIFMKQAQKPADVEHPYGHRQLESISAIVVGAFILTTGIAIFWESIN
                     KVYDILSKIETGHSASSWALVIAIFTFCLKIFLYTYTRKNMLKTNNPTLKALANDHLN
                     DIMASIAVIVGVLLGRLGYYWMDPAAGAIVAIYIIKTGIEIIMESSRELMDYLPDEDF
                     SREIRTEALAVEGVRSIEELGIHRFGPYYTVNMTITVDGAISVDDGNNISDKVEKRLL
                     DKFSSGLRQVHIHYHPYTEKISLSNNHPENKESQGVNI"
     gene            complement(3939..4934)
                     /locus_tag="PKM80_03790"
     CDS             complement(3939..4934)
                     /locus_tag="PKM80_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93062.1"
                     /translation="MEFSWISAKRCINIAIVGICKNAGKTTVLNYIVENYPHKWGILS
                     TGRDGETEDTLYKTPKPKVKLPAGCLFCADAKTLVQQGSGISILAKTKWQSGGRELWI
                     AKSEIDLETEITGPANVNGQIACAELFKKLGAAKVLIDGSLDRKSIVLSEAIDGIILA
                     AGASFGSQQAIIDELQRLITLSQIGTYHSSTLKKLTEQNKILIKSQNRWKTTALVSLI
                     ANETKLLEFINEINNPTHLYIPGAYTSSVNNRLGKHLKGIQLVFRHPECIKLSNIELE
                     VFLEDHKPLSLIPFKLKAIALNSHGVGSNEIDADEFHHNLRSVFNNYPLLDVMEI"
     gene            complement(4943..>5183)
                     /locus_tag="PKM80_03795"
     CDS             complement(4943..>5183)
                     /locus_tag="PKM80_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNV93063.1"
                     /translation="PVMPNYVISQMPGRVILRNYEGFITSYTEPEFAIQDESKYRELC
                     ADEYKSTEGIMSLMRGKKVAIGPAGTKRHQRRNHQ"
BASE COUNT         1654 a          985 c          941 g         1603 t
ORIGIN      
        1 atataccttt aaaggacatc gatcctgttt tcgattatta tacacccgta atcatcagca
       61 gcgaggattt tttggcaaaa aatcccgaga ccgcccagaa atttatgtca gcgttaaaaa
      121 agggttatga atattgtatt tccaagccag attcagcagc ggatatattg ctaaaaaatg
      181 tcccagagct aaatgcagaa caggttaggc gtagtatgca atatctggct aaagaatttc
      241 aaagcgatgc tcctacctgg ggaaggcaaa aaccgattgt ctggaaaaga ttttgtgact
      301 ggatgtatca gcaacgcctt attcagcttg cagttgaacc caataaagca tttactaacg
      361 attatctacc caaataataa aatgtccgat tgtattctgg aagctcaaga tatccgtaag
      421 agctttttct ttaagggttc actgattact gttctggata atgtacaatt tcacataagg
      481 acaggacaat ttgtaagtat catcggtcca agtggttgcg ggaaaagtac ctttctggaa
      541 cttttggctg ggatcactac accggatgag ggatttattt tactgaaagg caatgatata
      601 acagctaaaa caggtttcct ggggtatatg cctcaggacg atttactttt cccttggcta
      661 acagtaatga aaaatattct gcttccggtg aaagtaacag gtagcaacga acaagaagca
      721 aaacaaaaaa ttaatgaact gctacctcta tttggtttac agaattacac tgaacatttg
      781 ccttatcagc tttcgggagg attaaaacaa cgtgttgcgc ttttaaggac atatatgtgt
      841 agcgcagata ttttactttt ggatgagcca ttagccaaac tggatgctct aacacgtagc
      901 caacttcaat cttggctgaa agatatcgtc catttgttaa atttaacagt tatccttgtt
      961 acccacgata tagacgaagc tattatgctc tccgatagaa ttgacttgat gggtataaat
     1021 cccggaacat ttattgagag cataaaactg gatagaaata atcccctaac cgccgaagaa
     1081 caactgaaat tgaagattaa attacgcagc cagttagtga atggagaatc aaataatgat
     1141 aaaaaataat gtgtgatgcg aagattaata tcaagatggc tgttccacag atctaagttt
     1201 aaacaaggat tttaggatta gttcacgaag atataagttt agaaacaagg attttaggat
     1261 tagttcacga agatataagt ttagaaacaa ggattttagg atttaaggat taagaggatt
     1321 tatttataag ccaccgaaaa caccgaaaat agttaacatt gattacacag atttaagaga
     1381 taaaaaaaag atgacaaata ggtaatcgga gaacgccgtt cctccgcaag aaactactct
     1441 ctcacagatt acgcaatata ataggtttag gaggacagaa ttatcaggat ttaaggatta
     1501 agaggattta tttataagcc accgaaaaca ccgataatag ttcttacgga ttctattttt
     1561 ctttataccg agagcatcgc aactaccagt aacacagaga attatatata ggtaacaagt
     1621 cagaagccgt catcgctcta atttattatg ccttgttctc acacttattt agtaatgaag
     1681 gctcataact tagttcattg ccattctcaa tgcactaatt tcaaaatcca attgatccag
     1741 ctatctgcgt tctatctttt attttttagt gattagaggt ccgggatgtt tcaaattctg
     1801 catttcttgc agaattgttt gttgctgact tttgaatctg gccatctgtg tttcattcaa
     1861 tttttctgtt cccaccattt tcaagttagt cggattgata tacttcttcc ctttaattag
     1921 cccaaaatgt agatgtgcgc cagttgaacg tcctgtagaa ccaactctac caataatttg
     1981 tcctttttta acattttgtc ctttatgaac attgatagaa gatagatgcg catattgagt
     2041 tgaaagtcca ctggggtggt taatcctgat ttcatttccc caaccgccat tataacttgc
     2101 agttgtcact tttccacctg ctacagcata tacaggagtt ccataatgag ctcgataatc
     2161 aactccttga tgattagccc agcttccatg aaacggatca agacgtttac caaaagaaga
     2221 aactacatga attacagaaa ggggaaaacc gacaccattg ctgtgatagc ttttaccttc
     2281 tatagtgtaa agaccattga gcacagaatt ttcttctgca tcttcataac ggaagagttc
     2341 acaagtgcca gttctttctc ctgtatattg aacatagaag atctttctac cgggtagagt
     2401 tttcccttca aaaatgcgtt cttctacaaa aacaagaaac tggtcgccat tgcgagcatc
     2461 gtgggaaaaa tttatttctg tttccaagcc attattgata gcttgtttat cctgaggagc
     2521 aaagcccatt gttagcagag tggcatccaa agtttcctga atagttcctt caataatccg
     2581 tttagctttt ttaacaggta gggcctcaag atggtagata agagaatcgc cactaacagt
     2641 aaaatgatga cgagtagttg gttcctgtac aaacattaat ttctttatat gctgtttatc
     2701 ttccgatagt aatactttta gcgtatcccc cggttgaata gtcgttacat caatataatc
     2761 accaaagtta taagccagta aaccaatttc ttgaggagga acattctgat ttgctaaaac
     2821 ggaaaatata ctgcctccgg gaggaattat ttctattatc caaggttcag ttggtttaga
     2881 gatatcaagt ttaagttcag gaaggggttc agaattgatt ccgagtatac tgagcaagca
     2941 aaaagatgca agcaaaaaaa ggacaagaga aaaaagtata tcctttcccc aattcatata
     3001 ttaactcctt gtgattcttt attttccgga tgattattgg aaagagaaat cttttcggtg
     3061 taaggatgat agtgtatatg aacctgcctt aatccactgc tgaatttatc cagcaagcgt
     3121 ttttccactt tatcagatat attattgcca tcatctacac ttatcgctcc atctaccgta
     3181 atagtcatat taacagtata ataaggacca aaacgatgga taccaagttc ttcaatacta
     3241 cgaactcctt ccacagctaa tgcttcagtt cgtatttcac ggctaaaatc ttcgtctgga
     3301 agataatcca taagctccct ggatgattcc ataataattt caattcctgt tttaataata
     3361 tatattgcta caattgctcc ggcagctgga tccatccaat aataaccaag ccgacctaat
     3421 aagacgccaa caattacagc tatggacgcc attatgtcat tcagatgatc atttgccaag
     3481 gctttaagag ttgggttatt agttttaagc atatttttac gagtataggt atataggaaa
     3541 attttcaggc aaaaggtaaa gatcgcaata actaatgccc aagatgaagc agaatgtcca
     3601 gtttctattt tggaaaggat gtcatagacc ttattaatgg attcccaaaa aatggcaatt
     3661 ccggtggtaa ggataaaagc accaactaca atagcagaaa tggattccaa ctgacgatgg
     3721 ccataaggat gttctacatc agcaggtttt tgagcctgtt tcataaatat cttaactgca
     3781 atgtaataaa caacatcgga ggtggaattt attccatctg ctaaaagagc agcactatgg
     3841 ccaaaaatac ctacacaagt tttgataatc gataataata tattggagaa taaacccaaa
     3901 ttaacagtta cccgagttat tgaatcacga tcagccattt atatctccat cacatccaat
     3961 aagggatagt tattaaagac gctccgtaaa ttatgatgaa attcatcagc atctatttcg
     4021 ttactgccaa ctccatgtga attgagagct attgctttca gcttaaaagg aataagagaa
     4081 agcggtttat gatcttctaa aaacacttca agttctatgt tgctaagctt gatacattct
     4141 ggatgacgaa aaacaagttg aatacctttc agatgcttac ccaatctatt attcacacta
     4201 cttgtatagg cacctggaat ataaaggtga gtgggattgt ttatttcgtt tataaattcc
     4261 agcagtttag tttcgttggc aattagcgat actaaagctg tcgttttcca tctattctgg
     4321 ctttttatca ggatcttatt ctgctcagta agctttttta aagtagaact atggtaagtt
     4381 cctatttggg ataaagttat taagcgttgc aattcatcaa taattgcctg ttgagaacca
     4441 aaactggctc ctgcggctaa gattatacca tcaatagctt cacttaaaac aatagattta
     4501 cggtccagcg aaccatctat caaaactttc gcagcaccta attttttgaa tagttcagca
     4561 caggcaatct gtccatttac attagcagga cctgtaattt ctgtttcgag atcaatttcg
     4621 cttttggcta tccagagttc cctgcctccg ctttgccatt ttgttttagc caaaatacta
     4681 atgccagagc cctgttgaac aagggttttt gcatcagcac aaaaaagaca accagcaggt
     4741 aatttcactt tcggtttggg agttttgtaa agtgtatctt ccgtctcccc atcgcgtcct
     4801 gtacttaaaa ttccccattt atggggatag ttttccacaa tgtaattcaa gacagtggtt
     4861 tttccagcat ttttgcagat tcccacaatc gctatattaa tacaacgctt agcggaaatc
     4921 caggaaaact ccaaaaaagc tcctattgat ggttacgacg ctgatgacgc tttgtccctg
     4981 caggtccgat cgctactttt ttaccccgca ttaaagacat aattccttcg gtagatttat
     5041 attcatcagc gcaaagttca cgatatttac tttcatcttg tatagcgaat tcgggttctg
     5101 tgtatgaagt aataaaacct tcatagttcc gcaaaataac tcttccaggc atctgagaga
     5161 ttacataatt gggcataaca gga
//
LOCUS       DAORHB010000096         5163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_16766_length_5163_cov_3.192639, whole genome shotgun sequence.
ACCESSION   DAORHB010000096 DAORHB010000000
VERSION     DAORHB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16766_length_5163_cov_3.192639"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="PKM80_03800"
     CDS             complement(<1..1512)
                     /locus_tag="PKM80_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNV93064.1"
                     /translation="MKKTLLTLILAGVLLFAFAVPRNLVVVEIATGTWCPYCPGAAMG
                     ADDLITNSNPAAIIENHNGDNYANTYSNARNSYYGISGFPTAMFDGLNPVEGGSNTQS
                     MYPQYLTRVNARMAIPSKYTISATGSITGNTLSITANVAKPEDDSNSNIVLHCVITQS
                     HIQHNWQGQTHLNFVNRLMIPNQNGTPVSLATGGQQTYNLSGTFDPSWNINSCEAVLF
                     LQCNTTKEILQGVKYSLPGLLGSNPVSATELNFPDTYVSGHSTLDITLNNFFEFEVNG
                     TISSSNPVFTPIPATFTIQPYQSTTISVEFAPAAATNYTGTLTIDSNLSGYNQVQIPL
                     TGTGFNNTAPNITNIQISGPPVVYQQLSVSYDFADPDGDTEGETEFQWVQIINNQPVD
                     IEDATEQTYTIDIADIGWQLACRVTPKDQYGMPGTPVFSNYTLPIEELPPPQNLTATV
                     TAPNTVTLHWEKPNHFDNRGFVGYRIFRNSFILNNVMNVNTLEYVDTEIPDGTY"
     gene            complement(1554..2705)
                     /locus_tag="PKM80_03805"
     CDS             complement(1554..2705)
                     /locus_tag="PKM80_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV93065.1"
                     /translation="MNDRINSIFKEIKAEHESILGKRAFYNSNAWRNKKEEPDIMRSQ
                     FAVDRDRILYSGAYRRYHGKTQVFSFTNMIDEEMTNRNLHIAYVSQISRTIGKYLKLN
                     TELIEAIALGHDLGHCPFGHDGESALSRCCEQNDIGQFHHNIESLHIVDHISRKGKGL
                     NLTFQVRDGIISHDGEVHNTQLIPNTAKKEADIQSYITAKKIGEDTEFMPATLEGCVV
                     RITDTIAYIGQDIEDAIRFNILRRDELPKEQAVYLGNTNSQIIDTLIKSVIVNSFDED
                     FIAFDTETSEHLLALKKFNYRRIYTDEKVKKSNSIIYRTMPILFDIYLNDIKENNRES
                     KIFKHFLDHKNPEYLDNYSPAERVRDFIATMTDRYYNEEIKAYLLPWKG"
     gene            3056..3304
                     /locus_tag="PKM80_03810"
     CDS             3056..3304
                     /locus_tag="PKM80_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93066.1"
                     /translation="MIYTLPDGRQVDLSKVESISKIRDYGQVDNIVQKYLIGFSIRLT
                     NKEIIEVKDIYHYSDWSEVKLRMNKLREDIINKWKSMQ"
     gene            complement(3606..>5163)
                     /locus_tag="PKM80_03815"
     CDS             complement(3606..>5163)
                     /locus_tag="PKM80_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93067.1"
                     /translation="QTGQPLQQRTRVTARVTDLAGVVSIPDTNTVINFRVRPGFRPES
                     IIYPTKTYALTNHHFEDYGDDTTPELTVLPRTITQGSIHYATPLFKDFEGKYTAVYSP
                     NIKLWVRWGWWGEYGDVRDNSADYGDTTNLIAMPYGKKVDVVLDSKTRKNYFSEITAF
                     DIRFDGEPYNYPPFPFSQNGVTDQDGKRWLRIPTNSPLGQTVVLTSGQLPAPVGTGPG
                     KHTFEVRVVDLQDEYDHTPAVFTFYLHPYIEPANRNGVLIVDDDVDSPNQAPDALIDE
                     KYANMLSDYTGPVDVYSRRTHVKGKINAEEDIRGRSLAFSDLQKYKLVIYHSDNINDT
                     GNLLYELDGLSLFMMKGGNMVISHSHRLSPILSEIADSGTRPTFVTNLGFESNPKIGF
                     LNDNNRSAFFQNAVACMAGYENMHLQYGTPPSFLNMVNLKHGLGTLSYFEEGHYTGEA
                     IYKFGCKPVGYEIYPPTNDQYNFYNGKTVAFRKVNPSNSRAYVFGFPLSLMKDSETKA
                     MMNKILSEVM"
BASE COUNT         1579 a          895 c         1008 g         1681 t
ORIGIN      
        1 ataagttcca tctggaattt cggtatctac atattccaag gtatttacat tcatcacatt
       61 attcaggata aagctgttac ggaatatgcg ataaccaaca aatccacggt tatcaaagtg
      121 atttggcttt tcccaatgaa gagttacagt attgggagcg gttacggttg ctgtaagatt
      181 ctgaggtgga ggaagttctt caataggtaa tgtataattg ctaaaaacag gagttccagg
      241 cataccatat tgatctttag gggtaacacg gcaagcaagt tgccagccaa tatcggcaat
      301 atcaatagta taagtttgct cggtggcgtc ttctatatct actggttgat tattaataat
      361 ttgcacccat tgaaattcag tttcaccttc agtatcacca tcaggatcgg caaaatcata
      421 actgacggat aattgctggt atactaccgg gggtcctgat atctgaatat tagtaatatt
      481 gggggcagta ttattaaaac cagttccagt aagcggaatt tgcacttgat tataaccgct
      541 cagattacta tctatggtta aagttcctgt atagttagta gcggcagcgg gtgcaaattc
      601 tacagagata gtagttgatt gatatggttg tatagtaaaa gtagcaggaa taggtgtaaa
      661 aacaggattg gatgaagaaa tagttccgtt tacttcaaat tcgaagaaat tattcaaagt
      721 tatatccagg gtagaatgtc cgctcacata agtatcaggg aaattaagtt cagtagcgga
      781 aacagggttc gatcctaaaa gaccagggag cgagtatttt acaccttgca atatttcttt
      841 ggtagtgtta cattgcaaga aaagaactgc ttcacaggag ttgatattcc agctgggatc
      901 gaaagttccg gataaattat atgtctgctg tccgcctgta gcaaggctaa caggtgtacc
      961 attttgattg gggatcatta atctattaac gaaatttaga tgagtttgcc cctgccagtt
     1021 atgttgaatg tgggattgag tgataacgca atgcaaaact atattagaat tgctatcatc
     1081 ttccggtttg gcaacattag cggttatgct caaagtatta cctgtgatgg aaccagttgc
     1141 agaaattgta tatttggagg gaatagccat acgggcattt actctggtta gatactgagg
     1201 atacatagat tgtgtattgc ttccaccttc aacaggatta agcccatcaa acattgcggt
     1261 aggaaagccg ctaattccat aatatgaatt gcgtgcattg gaataagtgt tagcataatt
     1321 atccccattg tggttctcga taattgctgc agggttgcta ttagttatta agtcgtcagc
     1381 acccatagct gctcctggac aatagggaca ccaggttccg gtagcaattt ctacaactac
     1441 caggtttcgg ggaactgcaa aggcaaatag cagaacaccc gctaaaatca gtgttaacaa
     1501 ggtttttttc attggtactc cttatgtatg tgtgtttttt ttcttgcttt aggttatccc
     1561 ttccaaggaa gaagatatgc cttaatttct tcgttgtaat agcggtctgt catcgttgca
     1621 ataaaatctc gcactcgctc tgcgggtgaa tagttatcta agtattcggg atttttatga
     1681 tccaaaaaat gcttaaatat cttggattcg cgattgttct ctttgatgtc gtttaggtaa
     1741 atatcaaata atataggcat agttcgataa atgatggaat tggatttttt cactttctcg
     1801 tcagtataaa ttcttctata attgaacttt ttcaatgcta aaagatgctc actggtttca
     1861 gtatcgaagg caataaaatc ttcatcgaag ctatttacaa taacgctttt aatgagagtg
     1921 tctataattt gactattagt atttcccaaa taaactgcct gctctttggg tagctcatca
     1981 cggcgaagga tattaaatcg aatggcatct tcaatatcct gtccgatata agcaatagta
     2041 tccgtgatgc gaactacaca tccctctaaa gtggcaggca taaattctgt atcttcacct
     2101 atctttttgg ctgtaatata gctttggata tctgcttctt ttttagcagt attaggaatc
     2161 aactgtgtgt tatgaacctc tccatcatgt gaaataatac catcccgaac ttgaaaagtt
     2221 agattcaagc ccttaccttt tcgggaaata tgatctacaa tatgcaagct ttcgatatta
     2281 tgatgaaact ggccgatgtc attttgttcg caacaacgag ataatgcact ttcaccatca
     2341 tgaccaaaag gacaatgccc caaatcgtgc cctaaagcta ttgcctcaat aagctctgta
     2401 tttaacttta aatatttacc aatagtgcgc gatatctgcg aaacataagc tatatgcaaa
     2461 ttgcgattgg tcatttcttc atctatcata tttgtaaacg aaaaaacttg ggtttttccg
     2521 tgatagcgac gatatgctcc actatataaa attctatctc tatccacggc aaattgggag
     2581 cgcataatat ctggctcctc ttttttattg cgccaggcat tggaattata aaaagcacgc
     2641 ttgcctaaaa tgctctcgtg ctctgcttta atctctttga agatgctatt aattctatcg
     2701 ttcattaact actcctaatt gatataataa ttcaagtggg gtttactgtc aagcataact
     2761 tatatatttt ttgttgggga taaatcacac agattataat ttgcctataa atcatattag
     2821 catcaaggat ttttaatctg aagaagcatc gttttttatg tttgaatatt atttcattct
     2881 tggtatctgt ctgcagctat ataatttatc ctatttaaag cactataaaa ataggattag
     2941 ctgctaatta ctagatatta atattagatc tatattagtt attttaaaaa aaagattgac
     3001 agcaatatgg ctgcggttat aagaggtata ttagtatatg attaaggtgg aaaagatgat
     3061 ttatacttta ccagatggca gacaagttga tttatcgaag gtagaaagca tttctaaaat
     3121 tagggattac gggcaggttg ataatattgt tcaaaagtac cttattggtt tttctattcg
     3181 tttaacgaac aaggaaatta tcgaagtaaa agacatttat cattacagcg actggtctga
     3241 agttaagcta cgaatgaata aattgagaga agatataatt aataaatgga agagtatgca
     3301 ataaaatcgg taactgattt tcgttcttac ggaatataga cctcctagtc aactctttgt
     3361 taatattgcc ttatggaaaa aatctgcgtg cttacatatg agttttgccc ttcctaacag
     3421 gtggttcacg gaaatgaaat tgtttgcttt gaccagtctc aggaccggtg ttttatttaa
     3481 ttacattgaa taattgtcaa gagcagtgta cgaggcctgt atgcaaaagg ttctgtgaga
     3541 gagatgatgc tggaaaagat catccagcat catcctactc aattatagta tattgttaat
     3601 cgaaattaca ttacttcaga tagaatctta ttcatcattg ccttggtttc agaatctttc
     3661 ataagagaaa gagggaaacc aaaaacatag gcacggctgt tactgggatt tacttttcgg
     3721 aaggcaacag ttttaccatt atagaaattg tattgatcat ttgtgggcgg gtagatttca
     3781 tatccaactg gtttacaacc aaatttatat atagcttcgc cagtataatg cccttcttca
     3841 aagtatgaga gagtgcccag tccatgcttt aaatttacca tatttaggaa tgaaggaggg
     3901 gttccatatt ggaggtgcat attttcatat cctgccatac aggctacagc gttttggaag
     3961 aaggcagagc ggttattatc atttaaaaaa ccgatttttg gattgctttc aaatccgaga
     4021 ttggtaacga aagttggtcg tgttcctgaa tcagcaattt cagataaaat agggcttaat
     4081 ctatgtgaat gtgaaataac catattacca cctttcatca taaacaagct aagtccatct
     4141 aattcgtata agagatttcc tgtatcatta atattatcac tatggtaaat aacgagttta
     4201 tatttttgta aatcgctaaa agctaatgat ctaccgcgaa tatcttcttc ggcgtttatc
     4261 ttgcccttaa catgggtacg acggctatat acatctactg ggccagtata atcagacagc
     4321 atattggcat atttttcatc gatcaaagca tctggagctt gattagggct atcaacatca
     4381 tcgtctacaa tcaaaactcc attacggtta gcgggttcga tgtaaggatg aagatagaag
     4441 gtaaatactg caggggtgtg atcatattcg tcttgtaaat ctactactct tacttcaaat
     4501 gtatgcttac ctggtccagt accaactgga gcaggtaatt gaccactggt taatactaca
     4561 gtctgtccta aaggagaatt ggttggaatt cgtaaccacc gttttccatc ttgatctgta
     4621 actccatttt gactgaaagg aaagggtgga tagttataag gttcaccgtc gaagcgaata
     4681 tcaaaagcag ttatttcgct aaaatagttt ttcctggttt tactgtctaa aactacatct
     4741 actttcttac cgtaaggcat agcaattaga tttgtcgtat ctccataatc tgctgaatta
     4801 tcacgcacat ctccatattc accccaccaa ccccagcgga cccaaagttt tatgttagga
     4861 gaataaactg cagtatattt tccttcaaaa tctttgaata aaggtgtggc ataatggatt
     4921 gaaccctgag tgatagttct cggtagtact gttaattcag gtgttgtatc atcaccataa
     4981 tcttcaaaat gatggttagt taaagcataa gtttttgtcg gataaataat ggattcaggt
     5041 ctgaacccgg gacgaacacg aaaattaata actgtattag tatcaggaat agaaacaact
     5101 cctgcgaggt ctgtaacgcg ggcagttact cttgttcttt gttgtaaggg ctgccctgtt
     5161 tgt
//
LOCUS       DAORHB010000097         5152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_16813_length_5152_cov_4.095743, whole genome shotgun sequence.
ACCESSION   DAORHB010000097 DAORHB010000000
VERSION     DAORHB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16813_length_5152_cov_4.095743"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1882
                     /gene="alaS"
                     /locus_tag="PKM80_03820"
     CDS             29..1882
                     /gene="alaS"
                     /locus_tag="PKM80_03820"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNV93068.1"
                     /translation="MYTDLFMPIINRIEELSGVQYTIEQGMPHRVIADHIRCLCFALS
                     DGGFPSNEGRGYVLRRILRRAARYGRLLGFAEPFLHLLVPIVIEQMAHHFHELSGKED
                     YIKMVIKAEEERFNRTLDTGLEKFNEITKKLAGDIISGSDAFTLYDTYGFPLDLTAIL
                     AEEKGLKIDYQGFDKEMQEQKDRARKASKFTLQTSNEDWIELAENHPTEFVGYTETKI
                     KTNIQCYAVQEKGLILIQLAETPFYAESGGQVSDTGRIYNDGFEVVINNVRKQDDKIV
                     HYGKLIRGTINSEPVTAEVSIERRKCTARNHTATHLLHRALREVLGEHTVQKGSLVHP
                     DYLRFDFAHFKALTFEEIRKVEDIVNNAILDNRKVITTIQNIEEAKNEGAIALFGEKY
                     NNIVRVVCVEDFSKELCGGTHISFTGEIGLFKITSESSSAAGIRRIEAITGTTSLKWV
                     QDLQDKLNHLATMLNTPIKKLENKLDATLEQINSLEKEIKELKNKEGEKLVLELLSKA
                     RTQENYSLIIEQTSFANPDELKQFAEQLKSNMKSTIAVIFNLYQNKLNILCVVSSDLI
                     PKYNAGKIVAILASELNGKGGGRADLAMAGGKDTAKLSLVKEKVPELISSI"
     gene            1883..2869
                     /locus_tag="PKM80_03825"
     CDS             1883..2869
                     /locus_tag="PKM80_03825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV93069.1"
                     /translation="MNILILRLSSMGDIILTQPICALLQKSYPGCNIDYLCKESFIEL
                     PEMFDPPVNPIVYQKTLKFHLWLFKSKYDIVIDLHNKFATYLIMLFIKASQKVHYCKK
                     RKFRQRIVKGDKKLKIDSTVSLYASALTKLGIKQPWSYPRLQISSLKLSNSIYPTNQD
                     CKDIKPIKIAVFPGANHFTKRYPVLSWIQLINSNPQYCFTLFGSTMDKDICDNIRKHC
                     PGNCINKCSIMSLSELYRELSTFQVIISGDTGPMHLAATLNKPQISIFGGTHPRLGFR
                     PLNDKAIVLCADLPCQPCSLHGRVKCPEGHFNCMNSINPALLSSAIKKALSS"
     gene            complement(2990..3853)
                     /locus_tag="PKM80_03830"
     CDS             complement(2990..3853)
                     /locus_tag="PKM80_03830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HNV93070.1"
                     /translation="MLFSCNSKPKTEPTIEKSEAEHPESNTEKIENQDQEKTTSSPDI
                     TKIKSYKYTWSKSDNMPPIAIIIDDFGQYSGKLLEDFTSLPKEIAFAILPDLPHTQTV
                     ARLADKTGHQVLIHIPMQAIDHKANPGKRFIKNGMDKFAIADLLQDFYAQIPNAIAAN
                     NHMGSEVTSDIATMSIVLDELNELGIFFLDSATTNKSTTYTAAENLGLITAKRDIFLD
                     VPDNSDKTIINKIEGLAKYKGRNEPIVIITHCHNREKLVALQKFLAQMDDMGVQLISV
                     SQLYKKLRAPA"
     gene            complement(3877..4368)
                     /locus_tag="PKM80_03835"
     CDS             complement(3877..4368)
                     /locus_tag="PKM80_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNV93071.1"
                     /translation="MRKLILSVWLICVLCILFGCKRAEPQKKEPSSKIEFRQDGTLNI
                     SNPMGTVKATFSIEIVEKEEELMRGLKFRDQLGENQGMLFIFEYPDIYEFWMQDTYLP
                     LDMLFIASDGTINYIYENTVPFSEERITPNFTHKFVLEVNAGTVKKYNIQTGDKVTWK
                     RHS"
     gene            complement(4379..>5152)
                     /locus_tag="PKM80_03840"
     CDS             complement(4379..>5152)
                     /locus_tag="PKM80_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNV93072.1"
                     /translation="ELDPRPWDTFRITNADVDNNGSIDSFDSYLLCKYIYEPDYILPA
                     TSGIPEQVTIPIMNATYENNKLNLHFDDIDQLKSLVVSTAPNTINQVLHQGITFNHPF
                     VQAVNNNDGSYGFAGYAIDQDSLYIILGTNPQNFTLYYTVNGVAGSQYVNLSDAIDGS
                     DLPKPFTYLFQNNPNPFNPETTLHFDLAENNTPVTLNIYNLKGQLIRRLVSGVLSSGK
                     HSVVWNGLDDRGKEVSSGVYLYRLNTPNYQQTRKMLLSK"
BASE COUNT         1714 a          850 c          918 g         1670 t
ORIGIN      
        1 ataactctat gaggcattcc ctgttcaatt gtatactgat ctctttatgc ctataatcaa
       61 tagaattgaa gagctttctg gtgttcagta tacaattgaa cagggaatgc ctcatagagt
      121 tattgcagat catattcgct gtctctgttt tgccttaagc gatggaggtt tcccttccaa
      181 tgaaggaagg ggttatgttt taagaagaat tttaagaaga gctgccagat atggtcgatt
      241 acttggtttt gccgaacctt ttttgcattt actggtgcct attgtaattg aacagatggc
      301 acatcacttt cacgaactga gtgggaaaga agattatatc aaaatggtga tcaaggcaga
      361 agaagaacgc tttaacagaa ctttggatac cggcttggaa aaattcaatg aaataactaa
      421 gaaacttgct ggagatatta tatccggttc cgatgccttc actttgtatg atacatatgg
      481 ttttccctta gatttaaccg ctattttagc tgaagagaag ggcttgaaga tagattatca
      541 aggttttgat aaagaaatgc aagaacagaa agatcgtgcc cgtaaagcca gtaaatttac
      601 tttacagaca agtaacgaag attggattga gttggcagaa aatcatccta ctgaatttgt
      661 tggatatact gaaactaaaa ttaaaaccaa tattcaatgt tatgcagttc aagaaaaggg
      721 tttaatttta attcaacttg ctgaaactcc cttttatgca gaatctggtg gacaggtttc
      781 tgatacaggg agaatatata atgatggctt tgaagttgta ataaacaatg tgcgcaagca
      841 ggatgacaaa atagttcatt atgggaagct gataagagga actataaaca gtgaacctgt
      901 tactgctgaa gtatccattg aaagacgaaa atgtacagct cgaaatcata ctgctactca
      961 tctgttacat agagctttaa gagaagtttt aggagagcat actgtccaaa aaggttcttt
     1021 agttcatccc gattatctac gttttgattt tgcacatttt aaggcactaa cttttgaaga
     1081 aattagaaaa gtagaagata tcgtaaataa tgctattttg gataaccgaa aagtaataac
     1141 aactattcag aatatagaag aagcaaaaaa tgaaggagca atagctcttt ttggagaaaa
     1201 atacaacaac atagtaagag ttgtatgtgt agaggatttc tccaaagaat tatgtggtgg
     1261 aacacatatt tcctttacgg gtgaaattgg tttatttaaa attacctccg aaagctcttc
     1321 tgcagctggg ataagacgca ttgaagcgat aacaggaaca acctctttaa aatgggttca
     1381 ggatttgcaa gataagctaa atcatcttgc aacaatgcta aatacaccta ttaaaaaatt
     1441 ggaaaataaa ttggatgcaa ctttagaaca aattaactct ttagaaaagg aaataaaaga
     1501 actaaaaaac aaagaagggg aaaaattagt tctggaattg ctatccaaag caagaactca
     1561 agagaactat tcactaatta tagaacagac ttcttttgcc aatcctgatg agctgaaaca
     1621 gtttgctgaa cagctaaaaa gtaatatgaa aagcactatt gccgttatct ttaatctata
     1681 ccaaaataaa ctgaatattc tctgtgtagt tagttccgac ttgattccaa agtataatgc
     1741 aggaaaaatt gtagctatac tagctagtga attaaatgga aagggtgggg gaagagctga
     1801 tctggcaatg gctggaggaa aagatacagc taaacttagt ctcgtaaaag aaaaggtacc
     1861 tgaactaatt agttccatat aaatgaatat acttattctg cggctatctt caatgggtga
     1921 tattattctc actcaaccga tatgtgcttt gttacaaaaa agctatccgg gttgcaatat
     1981 agattatctt tgcaaagagt catttatcga gcttccagaa atgtttgatc ctcctgtaaa
     2041 tcctattgtt tatcaaaaga cgctgaaatt tcatttatgg cttttcaaga gtaagtatga
     2101 tatagttatc gatttgcata acaagtttgc tacctatctg attatgcttt ttattaaggc
     2161 ttcccagaaa gtgcactatt gcaaaaaacg caagttcaga cagcggatag tgaaaggtga
     2221 taaaaagcta aaaattgata gcacggtttc tctttatgcc tctgctttaa cgaaactagg
     2281 aattaaacaa ccctggtcct atcctcgttt acagattagc agtttgaagc tatcaaattc
     2341 catatatcct acaaatcaag attgcaaaga tatcaaacca attaaaattg ctgttttccc
     2401 tggtgctaat cactttacaa aaaggtatcc tgttctttct tggattcaat taataaattc
     2461 taatcctcaa tattgtttta ctctttttgg ttcaacaatg gataaagata tctgtgataa
     2521 tatcagaaag cactgtcctg gaaactgtat caataaatgt tccataatgt ctttgtctga
     2581 attgtataga gaactatcta cctttcaagt aattatcagt ggtgataccg gcccaatgca
     2641 tttagcagca actttgaata aaccgcaaat atctattttt ggaggaactc atcctcgttt
     2701 gggtttcaga cctttaaatg ataaagcaat agttttgtgt gctgatttac cttgccaacc
     2761 ttgttctctt catggaagag taaaatgtcc tgaaggtcac ttcaattgta tgaattcaat
     2821 taatcctgca cttcttagtt ctgcaataaa gaaagcgtta tcttcctgat ccttagtaat
     2881 ctacaatata aaacctatgc tcattaataa gtgatttcgt agcgaaattg aaagcaaaaa
     2941 agcagtggct ttatgcgcaa ctactttcaa atataattac aaaattgtat taagcaggtg
     3001 ctcttaattt tttataaagt tgtgaaacgc ttattaattg gacacccata tcatccattt
     3061 gagccaaaaa tttctgcaag gcaaccaatt tttcgcgatt gtgacaatgg gtaataatca
     3121 cgatcggttc atttctgcct ttatattttg ccaaaccttc aattttattg attatcgttt
     3181 tatcactatt atcgggaaca tccaaaaata tatctctctt agcggtaatc aatcctaaat
     3241 tttctgcagc agtgtaagta gtacttttat tggtagttgc gctatccaga aaaaatattc
     3301 ccaattcatt caattcatcc agaactatac tcatcgtagc aatgtctgat gttacttcac
     3361 tgcccatatg attattggct gcaatggcat taggaatttg agcatagaaa tcctgtagta
     3421 aatctgctat ggcaaattta tccattccat tctttatgaa tctctttccg ggattagctt
     3481 tatgatcaat tgcttgcata ggaatgtgta ttaaaacttg atgacctgtc ttatctgcaa
     3541 gacgagcaac tgtttgagta tgaggtaaat ctggtaaaat agcaaaagct atttctttgg
     3601 gaagggaagt gaaatcttcc agtaattttc ctgaatattg accaaaatca tcaataatta
     3661 tagctatagg aggcatatta tctgatttag accaagtgta tttataggat ttaattttag
     3721 taatatccgg agaagaggta gttttctctt gatcctgatt ctctattttt tcagtattac
     3781 tttctggatg ttcagcttcg gatttttcta tagttggttc tgtctttggt ttactattgc
     3841 aactaaataa cactatgcta aacaccaaga taactattag gagtgtcttt tccaagttac
     3901 tttatctcca gtttgaatat tatatttttt aactgttccg gcattaactt ccaaaacaaa
     3961 tttatgagta aaattggggg taattctttc ttcactgaag gggactgtat tttcgtagat
     4021 atagtttata gtaccatctg aagctataaa tagcatatct aaaggtagat atgtatcctg
     4081 catccaaaat tcatagatat ctggatattc aaaaataaac agcatacctt gattttcacc
     4141 aagctgatct ctgaatttta atcctcgcat cagctcttct tccttttcta caatttcaat
     4201 tgagaatgta gctttaacag ttcccatagg attagatatg ttcaaagttc catcttggcg
     4261 aaattcaatt ttactggatg gttctttttt ttgaggctct gctcttttac aaccgaatag
     4321 tatacataaa acacagatca accaaaccga gagtattaat tttcgcattt atatcgtact
     4381 atttagatag cagcatttta cgagtttgct gatagtttgg agtgtttagt cgataaagat
     4441 aaactccaga actaacttct ttgcctctat catctaaacc gttccaaacg acactgtgtt
     4501 ttccagagga taaaacgcca gaaactaacc gacgaataag ctgtccttta aggttataga
     4561 tgtttaaagt aacaggtgta ttgttttcag caagatcaaa atgcagagta gtttcgggat
     4621 taaaaggatt ggggttattc tggaaaaggt aagtaaacgg ttttggtaaa tcagaaccat
     4681 caatagcatc actcaaatta acatattgag aacctgctac tccattaact gtatagtata
     4741 aagtaaagtt ctgcggattg gttcctaaaa ttatatataa agaatcctga tcaattgcgt
     4801 aaccggcaaa cccgtaagaa ccatcattat tattaactgc ttgcacgaaa ggatggttaa
     4861 aagtgattcc ctgatgcaaa acctgattaa tagtattcgg agcggtagaa actactaggg
     4921 atttcagctg atcgatatca tcaaaatgca agtttagttt gttgttctca taagtagcat
     4981 tcataattgg aatagtcact tgttctggaa ttccagatgt agcaggtaaa atataatccg
     5041 gttcatagat gtatttacaa agcaaataac tatcgaaaga atcaatacta ccattattgt
     5101 ctacatcagc attagtaatt ctgaaagtat cccagggtct tggatcaagc tc
//
LOCUS       DAORHB010000098         5139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_16872_length_5139_cov_4.483478, whole genome shotgun sequence.
ACCESSION   DAORHB010000098 DAORHB010000000
VERSION     DAORHB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16872_length_5139_cov_4.483478"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKM80_03845"
     CDS             complement(<1..313)
                     /locus_tag="PKM80_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93073.1"
                     /translation="MAYGLFIDLRAIQDYIFASNRLKDNIGASYLIKHLLEEFLTDQG
                     YCGGGNLFLLCKDEKEAKETIYSLSRKILNECPGLSFSAVYEENFNLDDFPAAFSLLQ
                     EK"
     gene            complement(316..1446)
                     /locus_tag="PKM80_03850"
     CDS             complement(316..1446)
                     /locus_tag="PKM80_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93074.1"
                     /translation="MVEVLILVGTNPVPCYVSAIYLKNHYNNSRISLICSKENKTLGQ
                     TGTEDIGQRIKTQLIKEGKFKEEDFNQIISIDDVTSFNSIECKLNKYINEEDKKAIHL
                     NFTGGTKTMAAFCYYYLKSHYKSFSSSYLDSRRKIMIIDGNVADSQIDLRQDPMLAIN
                     LETMAVLQNQTDIKPEEYKEKYPELLEKLIDLLDNGNLEEIYGKRNNDLEHMANQLNI
                     TPEKNRLLESVNIKYEELNRNQREDLEDFINGRWLEYYVYNELIKLTGNIQFYFDVHN
                     NQYQVDIVAIYGYQLTLISVTTHKTKPLCKLKAFEAITRATQLGGEEAKTILISRTDE
                     PDNLQRDVEKYIGTLVPNFRAFGETQLKDKSIFPAIINYIKE"
     gene            2061..3647
                     /gene="secD"
                     /locus_tag="PKM80_03855"
     CDS             2061..3647
                     /gene="secD"
                     /locus_tag="PKM80_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNV93075.1"
                     /translation="MKKFSWRGLSIVIFICVTAFFLAPLAIPNLPSWWSKHHLKLGLD
                     LRGGMQILLEVDTSELSATDARNAVEQNIKIIRERIDQFGVAEPTIQKLGEKRIMVQL
                     PGVSDYKAAENLIKQTAMLEFKLVAPSEEAKRVIDAIDKNISGNLDKFPALAELAKKD
                     KSIIADTTSTDSLQVGTGIFSSLIRPGEMDFEVQYSDVRLIQELISDTLFAQFVPIGY
                     QLALDKADTENPRADRALYVLNSAPQLTGADLAKAKVEYGSATSTDPRVANKPYISIE
                     MKREGARKFERVTADNVGKRLAIVLDGVVYSAPNIQERIAGGRAQITGRFTATEANEL
                     AIVLSTGNLIAPIKPVSTSIIGATLGSDSIRKGTFAGLIGLIIVALFMVIYYKGCGFI
                     ADIALVLNVGFILAMLTAFGATLTLPGIAGIILTIGMAVDSNVLIYERIREELDIGKA
                     PRTAVDAGFKRAIITVWDSNLTTLIAAIVLYYLGTGPIRGFAVTLAIGIMSSMFTAIV
                     MVRAILDNFVLTGTKTKLSV"
     gene            3660..4643
                     /gene="secF"
                     /locus_tag="PKM80_03860"
     CDS             3660..4643
                     /gene="secF"
                     /locus_tag="PKM80_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNV93076.1"
                     /translation="MRILKNPNIQFVKYRYWAYAFSIVVLLIGLGGIIFNGLNWSIDF
                     TSGIAATLNLKAKDPNVAPLPIDELRSVLAKNGFPDAEIMYVGDEANSVFQIKIKTTG
                     KGSNISAEARSKLIDIIQSEFPEYVKGRDINKEVIEEIYEVGPKVGSELRTQAFWAVL
                     LALIAMIIYIWFRFELTFGLMGILALFHDVFIIVGIFALTGKEITMQIIAALLTIVGY
                     SINDTIVIFDRIREDLKKNRKDPMPVIFNRSINETLSRTIITGGTTFITSACLFFFGG
                     SVIHDFAFAICLGILFGTYSSIFVASNLVIDLHKITGKEKKTLQHLTKKKK"
BASE COUNT         1571 a          881 c          932 g         1755 t
ORIGIN      
        1 acttttcttg cagtaaactg aaagcagccg ggaaatcatc tagattaaaa ttctcttcat
       61 aaactgcgct aaaacttaaa ccagggcatt catttaagat ttttcgtgag agtgaataga
      121 tagtttcctt tgcttctttt tcatctttac agagcagaaa aagattacca cctccacaat
      181 aaccctggtc agttaggaac tcctccagaa gatgcttaat taaatacgaa gcaccaatat
      241 tatccttaag gcggttagag gcaaaaatat aatcctgaat tgctctaaga tcaataaata
      301 aaccataagc cataattact ccttaatata atttataata gctgggaaaa tagatttatc
      361 ttttaattgt gtttctccaa aagccctaaa attgggcact aaagttccga tatatttttc
      421 tacatctctc tggagattat ctggttcatc cgtacgcgaa ataagtattg tttttgcttc
      481 ttctcctcct aactgcgtag ctcttgtaat ggcttcaaag gcctttaact tgcacaatgg
      541 ttttgtttta tgtgtagtaa cagatattag ggtaagttgg tagccataaa ttgcaactat
      601 atctacctga tattgattat tgtgaacatc aaaataaaat tgaatatttc cagtgagttt
      661 aataagttca ttgtagacat aatattccag ccatctacca ttaatgaaat cttccaaatc
      721 ttctctttgg tttctattta attcctcgta ctttatattt acactttcta acaaacgatt
      781 tttttctggt gttatgttca attgattggc catatgttcc aggtcattat tccttttccc
      841 atagatttct tctaaattgc cattatctaa caagtcaatt agtttttcca ataattcagg
      901 gtatttttct ttatattcct ctggcttaat gtctgtttga ttttgtaaaa ctgccatagt
      961 ttctaaattt atcgccaaca tagggtcttg gcgtaaatca atttgcgaat ctgctacatt
     1021 accatcaata atcataattt tgcgtctgct atctaagtat gaagaactaa aagatttgta
     1081 gtggcttttt agataatagt aacaaaatgc tgccatagtt ttagttccgc cagtaaaatt
     1141 taaatgaatt gcttttttat cttcttcgtt tatatattta tttaacttgc attcaattga
     1201 gttgaaagaa gttacatcat caatagaaat tatctgatta aaatcctctt ctttaaattt
     1261 gccttccttt ataagttgag ttttgattct ttgcccgata tcttctgtac ctgtttggcc
     1321 taaggtctta ttttcttttg aacatattaa gctaatcctg gaattattgt agtggttctt
     1381 taaataaatt gcacttacat aacatggaac cggattggta cctaccagta ttaaaacttc
     1441 aaccataatt agcttccttt catattagca tatatagctt attttgaaga aaagataaat
     1501 tctgtacaat agtgtttaga tagaattgca cttatctgcc actatcctaa caaaacatag
     1561 tatcttaata gaaacggact ctctaactat attatatatc atagaactat ataaaacaat
     1621 tataagtatt atgtcaagtg ataaaaagaa aaaatcataa tttattgtaa cagtatagga
     1681 atgttaatat tcaaccgctt aaaaaaacct atgctactca tactgtgaga cgatcataat
     1741 tatctatgtg gcctgtaggc aaatgtatac aagttgttaa agattttcag ttttttttaa
     1801 ttggtttata gtacaatatg ctaaagaaat atatgcaaaa gcagtgatta tacagattca
     1861 tatatttatg agagtgctta ttttgacata tttgctaata ttacttggaa tggagaatca
     1921 taataaatga agagtcaggc agctgaatta gcatattgtg caaaaacatt tcccttgacg
     1981 aatggagggt tatcagagtt ttggcttaat aatgtaaaat ctttaatatg ataaaaaatg
     2041 tataagggag cataaagcta atgaagaagt tttcctggcg tggcctttct atagtcatat
     2101 ttatatgcgt gacagcattt tttttggctc ctttggcaat tcctaatcta ccaagctggt
     2161 ggtcaaaaca ccatttgaaa ctcggtttgg atttaagagg tgggatgcag atacttctgg
     2221 aagttgatac ttcagagctt tctgcaactg atgccagaaa tgctgttgag cagaatatta
     2281 agatcattcg tgaacgtatt gatcagtttg gagtagccga acctactatc caaaaattag
     2341 gcgagaaaag aattatggtt caactgcctg gtgtttccga ttataaggca gctgagaatt
     2401 taattaaaca aactgctatg ttggagttta aacttgtagc tccgtcagaa gaagctaagc
     2461 gtgtaataga tgctatcgat aaaaatatca gtggtaatct ggataagttt ccagctttgg
     2521 cagaattagc caagaaggat aaaagcatta ttgctgatac aacttccact gatagcttac
     2581 aagtaggaac cggtattttc agttcgttga ttcgtcctgg tgaaatggat tttgaggttc
     2641 aatatagcga tgtgcgttta attcaagaac tgatttctga tactttattt gctcaatttg
     2701 tgccaatagg ttatcagctt gctttagata aagcagatac cgaaaatccc cgagcagata
     2761 gagctttata tgttctgaat tcagctcctc agcttacggg agcagatctt gcgaaagcta
     2821 aagtagaata tggttcagcc acatctaccg atccgcgagt tgccaataaa ccctatattt
     2881 ctatagaaat gaaaagagaa ggggctcgta aatttgaacg cgttactgct gataatgttg
     2941 gtaagcgttt agcgattgtt ttggatggtg tagtttattc tgctccgaat attcaggaaa
     3001 gaattgccgg tggtagagct caaattacag gaaggttcac agctactgag gcaaacgaac
     3061 tggctattgt actcagcact ggtaacttga tcgctcccat caaaccagta tctacttcta
     3121 ttattggtgc tactttaggt agtgatagca ttcgtaaagg gacttttgct ggtttaattg
     3181 gtttgataat tgtagccttg tttatggtta tctattataa aggatgcggt tttattgctg
     3241 atattgcctt ggttttaaat gtgggcttta ttttagctat gttaactgcg tttggtgcaa
     3301 ctttaaccct tcctggtatt gcaggtataa ttcttactat tggtatggca gtggattcca
     3361 atgttcttat ttatgaacga ataagagaag aattggatat agggaaagct cctcgtacag
     3421 cagtagatgc aggttttaag agagctatta ttacagtttg ggattcaaac ttaactactc
     3481 ttattgcggc aattgtgctt tattatcttg gaactggtcc aatccgtggt tttgctgtaa
     3541 ccttagctat cggtattatg agttcaatgt ttactgccat agtgatggtt agagccatac
     3601 tggataattt tgttcttacg ggaacaaaaa caaaactgag cgtgtaagga gttattataa
     3661 tgcgaatcct caagaatcct aatatccaat ttgtgaaata tcgttactgg gcttacgctt
     3721 tctcaatcgt tgtgctcttg atcggcttgg gaggtattat attcaatggc ttgaattgga
     3781 gtatagattt caccagcggt atagccgcaa cattgaactt gaaagctaaa gatccaaatg
     3841 tagctcctct tcctattgat gaattacgct ccgttcttgc caaaaatggc ttcccggatg
     3901 cggaaattat gtatgttggc gatgaagcca attctgtttt ccaaatcaaa ataaaaacaa
     3961 caggtaaagg cagtaatatt tcagcggaag ctcgatcgaa attgattgat atcatccaaa
     4021 gtgaatttcc tgaatatgtg aaaggtcgag atatcaataa agaagttata gaagaaatat
     4081 atgaagttgg tcccaaagtt ggtagcgaac ttcgaactca agcattttgg gcagttctgc
     4141 ttgccttgat tgcaatgatc atttatattt ggttccgttt tgaattaact tttgggctaa
     4201 tgggaatttt ggctttattc cacgatgtgt ttattattgt gggtattttt gctcttaccg
     4261 gcaaagaaat cacaatgcaa attattgccg cgcttttaac aattgtgggt tacagcatca
     4321 acgatacgat tgtgatcttt gaccgtattc gcgaagatct gaaaaagaat cgtaaagacc
     4381 ctatgccggt tatattcaat agatctatca atgaaaccct ttccagaaca ataattacag
     4441 gtggaactac ttttattact tctgcttgtt tatttttctt tgggggtagt gtgattcacg
     4501 attttgcttt tgccatttgc ttaggtattc tttttggaac ctattcttca atctttgtag
     4561 ccagcaacct ggtgatagat ttacataaaa ttacaggtaa agaaaagaaa actttgcaac
     4621 acttaactaa gaagaaaaaa taaccccata caatctacct cctctcgaac cgctcatctt
     4681 ttgagcggtt ctttctttga tcttctacac cgagaacact gaacaaagat aacgacacgc
     4741 aaatagaatt ttgagagcta tcgaaagtac cgaaagaaac aggagcacag aaaaaaaata
     4801 ggaaggaaag aaaaggtaga aaagtaattt agattcttgt tacttgcttt caactcttag
     4861 ctttttgcct tcaacttgct ctatggcttc tacttatagc ttccagccca ttcttgtttc
     4921 acttttacac ttttttgcgg aggaacggcg tcctccggct acacaaaaaa cagaggaata
     4981 gcgtcctcaa actacctgta agatttgcac ctaaaactat ctccgggttt cacttttcca
     5041 ctttctacct tcctcttcta actatgaact tcaaaaaagc tctccagtcg cagcagtgtt
     5101 ctggcatcga tatcttcagg ttgatcacct tccagagtt
//
LOCUS       DAORHB010000099         5096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_17107_length_5096_cov_3.264630, whole genome shotgun sequence.
ACCESSION   DAORHB010000099 DAORHB010000000
VERSION     DAORHB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17107_length_5096_cov_3.264630"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="PKM80_03865"
     CDS             <1..1591
                     /locus_tag="PKM80_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV93077.1"
                     /translation="INAPNFYPFNTTITATAEQMPYIVCNHISFNDQDGLYHSGDTLT
                     MDFYIKNVGLLDLTTPGTLTLLSNSPNIQITSSTANFDGISVGDSLAINAAFQFNVIG
                     NYPDHTNAMLQIRANYDSFTSTSIAYITLNAPIIDLTSYQIHNSSLIIMPGDSPTINF
                     SISNTGSGNAYNPILILFSSYSDIILDQYEVVLDPIYANTTLNFQNVISFHILDSASI
                     DDNYTIDYIFSADNGNVIEGSFLVHLGAISYSFETNYQDWETVALSSNFINQWHRSST
                     RNHTPNGTYAMKFGGNGSGQYAGSTYGALISPVMNVNPNCQLKFYHWMDAEKHSTNQS
                     YAWDGGLVEMSLNGGDWTQITPVGGYPYKIYNNSASPFPANTWVYSGTINWSEAIFEL
                     GNISGTAQFRWIFGSDAYVGGEGWYIDDVNITGIVDNIDETTPSLENVVLHNNYPNPF
                     NPETTIEFQLPSKMPICLEVYNSKGQLVNRLIDDIMDAGIHKVVFTGLDMHKHNIGSG
                     VYYYRLRTPSGTVTHKMLLIK"
     gene            1597..2310
                     /locus_tag="PKM80_03870"
     CDS             1597..2310
                     /locus_tag="PKM80_03870"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNV93078.1"
                     /translation="MLKLTPNTKVVVLTGAGISAESGIRTFRDSDGLWEDHRVEDVAT
                     PEAFFSNPVMVWNFYRERYNDSLSRIPNPAHLALVKLENFLGDNFLLVTQNIDRLHHR
                     AGSQRVLEMHGSLSNCLCTKCQSKLAIKDIDLNKAIPLCPYCQGFLRPDIVWFGEFPH
                     YLDMIDDALRECSVLIVIGTSGVVYPAAGFVITAKLMGARTVAINLEKPDNLGYIDEF
                     YQGKSGELLPKLVDMWISG"
     gene            2632..3921
                     /locus_tag="PKM80_03875"
     CDS             2632..3921
                     /locus_tag="PKM80_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV93079.1"
                     /translation="MRKLFILAITIFVLLAGCKSALQSEKVKITFWHGLSGPLGDTLN
                     EMIREFNRTHKNVEVITNPISSYTALSQKLMASIQAKKQPDIAQVFESWTSKYIEAGV
                     LCNFEELMRADTTWTKEDLEDIYPVFLKSNTFNDTIYSFPFNKSVRAYFYNKDAFYRA
                     GLDPNHFPATWNEFRLYCRKLTTDTNNDGIPETYGTNFNVNEWQFINLLYQAGGAILD
                     ELGNPQLNSPQGIEALTFITDLLNKDKTVYLVREFEGQNDFLAGIAAMYEGSSVSITH
                     MRQQPINFNIGYAPLPTYRTNKSAVSGTNIVIFKSGDKKREQAAWEFIKWFTDTNQTA
                     RWSADTCYMPVRKSAMQSAILKNFLKDYPQFQGIYNQLETAVFEPQIPAWFKARLELK
                     GYLEKAMRGISTPKEALDEMNEKFKSIIYEEQYIKNL"
     gene            3953..4597
                     /locus_tag="PKM80_03880"
     CDS             3953..4597
                     /locus_tag="PKM80_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HNV93080.1"
                     /translation="MLNCAEELILLAIEDKTNTFYKITNINFNVALIGALLMDLALRK
                     RIDVDMEGINILSTEKTGDKFLDTVLENLMNSETGNEPANLVAQLYNSMGHLKNKLLQ
                     DLEDKGILKVSQCKVFWLFKQRRYPVIDQKEEEEVLSRIRKTVLTDIEPEPRDLALIA
                     LLNICNLMDKIFTQEELEQRHNRLEQLKKMDLISPAVKRIIGEMQVLITTSYTT"
BASE COUNT         1681 a          849 c          920 g         1646 t
ORIGIN      
        1 tatcaatgca cctaattttt atccttttaa tacaacaatt actgctacag ctgaacaaat
       61 gccatacata gtatgtaacc atatcagttt caatgatcaa gatggtcttt atcatagtgg
      121 tgatacttta acaatggact tctatattaa aaatgtagga ttgcttgatc ttacaactcc
      181 tggaacatta actttactaa gtaattctcc caatatccaa attacttctt ccactgctaa
      241 ttttgatggt atttctgtag gggattcttt agctatcaat gctgcctttc aatttaatgt
      301 tattggaaat tatcctgatc atacaaacgc tatgttacaa attagagcta attatgattc
      361 gttcacctct acttctattg catatataac attaaatgct cccataatag atttaacatc
      421 atatcaaatt cataattcca gtttaataat tatgccagga gattcaccaa caatcaattt
      481 cagtatatct aatactggaa gtggaaatgc ctataatcca atattaatac tattcagctc
      541 ctatagcgat ataattctgg atcaatatga agtagttctg gatcccattt atgctaatac
      601 tactttaaat tttcagaatg taatatcctt ccatatttta gatagtgcat ctatagatga
      661 taattacact atagattaca ttttcagcgc agataacgga aatgtgattg aaggtagctt
      721 tttagttcat cttggtgcca tttcatacag ttttgaaacg aactatcaag attgggaaac
      781 tgttgcactc agcagtaatt ttattaatca atggcatcgt tcatctactc gaaatcacac
      841 tcctaatggt acttatgcta tgaagtttgg aggtaatggt tctggacaat atgctggtag
      901 cacttacgga gcacttatta gtcctgttat gaatgtaaat cccaattgtc aattaaagtt
      961 ctatcattgg atggatgcag aaaagcacag tacaaatcaa tcttatgctt gggatggtgg
     1021 tttggttgaa atgagtttaa atggaggcga ttggactcaa attactcctg tgggcgggta
     1081 tccttataaa atatacaata attcagcatc tccttttcct gctaatactt gggtatattc
     1141 aggtactatt aattggtctg aagccatttt tgaacttggc aatattagcg gcacagctca
     1201 atttcgttgg atttttggca gtgatgctta tgtaggtgga gaaggttggt atatcgatga
     1261 tgttaatatt acaggtattg ttgataatat agacgaaact acaccttccc ttgaaaatgt
     1321 tgttctgcac aataactatc ctaatccctt taatccagaa acaaccattg aatttcaatt
     1381 accctcaaaa atgcctatat gcctcgaggt ttataacagc aagggacaat tggtaaaccg
     1441 tttgatagac gatataatgg atgcaggaat tcataaagtt gttttcactg gtttggatat
     1501 gcacaagcat aatattggaa gtggagtata ttattaccgt ttaaggactc cttctggtac
     1561 tgtaacccat aaaatgctac tgattaaata aaaaagatgc taaaacttac acctaatact
     1621 aaagttgttg tattaaccgg tgctggaata agtgccgaat ccggaattcg tacttttcgt
     1681 gatagtgatg gattgtggga agatcataga gttgaggatg tggcaacccc agaagcattt
     1741 ttttcaaacc ctgttatggt ttggaatttt tatcgggaaa gatataatga tagtttatcc
     1801 cgtataccga atcctgccca tttagcattg gtgaaactgg agaacttttt aggtgataat
     1861 tttctgttgg ttacccaaaa tatagatcgt ttgcatcata gggcaggttc acagagagtt
     1921 ttagaaatgc atggttcgct ttctaattgc ctttgtacaa aatgccagag taaattagct
     1981 atcaaagata tagatttaaa caaggcaatt cccttatgtc catattgcca gggattttta
     2041 agaccagata tagtttggtt tggtgaattt ccccactatt tggatatgat tgatgatgcc
     2101 ctaagagaat gttctgtgct aatagtaatt ggaaccagcg gtgtagtata tcccgctgcg
     2161 ggatttgtaa ttactgccaa acttatgggt gcccgtactg tagctattaa tcttgaaaaa
     2221 cctgacaatc ttggttatat agatgaattc tatcaaggta aatctggaga attattaccc
     2281 aaattagtag atatgtggat tagcggataa ataggaccca gctaccatag cttaaatcga
     2341 acgaatattg atatataact atgcagtgca tagatttaaa aggactctgg aataaaataa
     2401 ttattggtaa tggcgtcaat gaagagtaag caacatgagt tttatatagt gtagtgttat
     2461 tttcattctc cattgtgaat acgcaattat tcattataca tatattttta ctatgttagc
     2521 tttcctttgt atacctctaa aagtaatatc taacactttt tcattgactc caactccctt
     2581 taaataataa tgacggtagg ttcgtttttt tataaaagaa aaggagtaac tatgcgcaag
     2641 ctgttcattt tggcaataac gatctttgtc ctgttagcgg gttgtaaaag tgctttgcag
     2701 tccgagaaag taaaaattac tttctggcat ggtctaagtg gtcctttggg agatactctt
     2761 aacgaaatga tccgagaatt taatcgcact cataaaaatg tagaagttat aaccaatcct
     2821 attagtagtt acacggctct ctctcaaaaa cttatggctt ccattcaggc aaaaaagcag
     2881 cctgatatag ctcaagtttt tgaatcttgg acttccaaat acatagaggc aggggtactt
     2941 tgtaattttg aggagttaat gcgcgcggat acaacctgga caaaagaaga cttagaagat
     3001 atctatcctg tttttttgaa atccaataca tttaatgaca ctatctattc ttttcctttt
     3061 aataaaagcg ttcgtgccta tttctataat aaggatgctt tttatagagc tggacttgat
     3121 cccaatcatt ttcctgctac ctggaatgaa tttcgtttat attgtcgtaa attgactact
     3181 gatactaata atgatggcat acctgaaacc tatggcacaa atttcaatgt gaatgaatgg
     3241 cagttcatca atcttctcta tcaggcagga ggagctatat tagatgaact aggtaatcct
     3301 caactaaata gccctcaagg aattgaggca ttaactttta ttaccgacct cctgaataaa
     3361 gataagaccg tatatttggt tcgtgaattt gaaggacaaa atgacttctt agctggaatc
     3421 gctgctatgt atgaaggaag tagtgtatcc attacacata tgcgtcaaca accgatcaat
     3481 tttaatattg gatatgcacc gctacctact tatagaacaa ataaaagtgc cgttagcggg
     3541 accaatatcg tgatttttaa aagtggtgat aaaaaaagag aacaagctgc atgggaattt
     3601 atcaaatggt ttactgatac caatcaaaca gcccgatgga gtgctgatac ttgttatatg
     3661 cctgtacgaa agagtgctat gcaatcggca atactgaaaa attttctgaa ggattatcct
     3721 cagtttcaag gtatttataa ccaattggaa acagctgttt ttgaaccaca aattcccgct
     3781 tggttcaaag ctcgacttga actaaaggga tatctggaaa aagcaatgcg tggaatatct
     3841 acccccaaag aagcgttgga tgaaatgaac gagaagttta aatcaatcat ctatgaggaa
     3901 caatacatca agaatctgta atataacctg tttaaataaa aaggagatta aaatgcttaa
     3961 ctgtgccgaa gaattaatcc tgctcgctat agaagataaa actaacactt tttacaagat
     4021 aaccaatatc aacttcaatg tggctttaat tggtgccctt ttaatggact tggcattgcg
     4081 aaaaagaatt gatgtagata tggaaggcat taatatccta tctaccgaaa aaacaggaga
     4141 caaatttctg gatactgttt tagaaaattt gatgaattcg gaaactggta atgaacctgc
     4201 taatttagta gctcagcttt ataattctat gggtcattta aaaaataaac tactgcaaga
     4261 tttagaagat aaaggcatcc ttaaagtaag ccaatgtaag gttttttggt tatttaaaca
     4321 acgccgttat cctgttattg atcaaaaaga agaggaagaa gtcctttccc ggattcgaaa
     4381 aactgtgctt acagatattg aacccgaacc gagagattta gctcttatag ctttacttaa
     4441 tatctgcaat ttgatggaca aaatttttac tcaagaagaa ttagaacagc gtcacaatcg
     4501 cctagaacaa ttgaaaaaaa tggatctgat cagcccagca gtaaaaagaa ttataggaga
     4561 aatgcaggtt ttaattacaa cctcctacac tacctgatat ttactcttta agattgtagc
     4621 aactgaattg ccgaattcat atcatctaca aaacaggtaa acacaaggtc cgttggcaaa
     4681 cttcggttta ttcggcaagc aagaattggt aagctctttt taccctaaac ggtgatattt
     4741 gggattgata tcaagtacaa tccgaatata ttaacctacc ataaggtgtt tactacttta
     4801 gtataaactg catattgtat gcaagatccg gtattcgctc ttaagcgaaa gcgttaatca
     4861 agtgttaatc taaccttaat caagcgttaa ttattaacgc ttgattaagg cctgttaaac
     4921 gagagattaa aaccttcaag caaggaaaac aagcgacaat aatattggga ttttattatc
     4981 aaattgattg ttacagttag tataattcag tttcaagagg gaaataatcg caatattttg
     5041 aagttgcaac tttaatcctc ccaattgtaa ttgatcctta tatgaatctt aagagg
//
LOCUS       DAORHB010000100         5056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_17309_length_5056_cov_3.648470, whole genome shotgun sequence.
ACCESSION   DAORHB010000100 DAORHB010000000
VERSION     DAORHB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17309_length_5056_cov_3.648470"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PKM80_03885"
     CDS             complement(<1..1101)
                     /locus_tag="PKM80_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNV93081.1"
                     /translation="MKNPDFLKIKPDFNAEQKTEPKLDFSWKTNEQIEVKPLFFKADM
                     ENIEHLDYLSGLPPFLRGPYPTMYIQRPWTIRQYAGFSTAEESNAFYRRNLAMGQKGL
                     SIAFDLPTHRGYDSDHPRVIGDVGKAGVAIDSILDIKILFDQIPLDKMSVSMTMNGAV
                     IPIMAFYIVAAEEQGVKPEALNGTIQNDILKEYMVRNTYIYPPEPSMRIIADIFSYTS
                     KKMPKFNSISISGYHMHEAGATADLEMAYTLADGLEYVRTALKAGIDIDVIAPRLSFF
                     WAEGMNYFMEVAKLRGARILWAKIIKQFNPKNPKSMALRTHSQTSGWSLTEQDPYNNI
                     TRTCIEALAATMGHTQSLHTNSLDEAIALPTDF"
     gene            complement(1098..2990)
                     /locus_tag="PKM80_03890"
     CDS             complement(1098..2990)
                     /locus_tag="PKM80_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNV93082.1"
                     /translation="MDNDNKLNLLKEFPPPTYEEWLQVVNEGLKGADFEKVMKTQTPE
                     GITLQPIYRKEDVAKLPFMESIPGKSPYVRGNNPIRFLTEGWLIAQNHTDSDLNTLNQ
                     KILKELNQGLTAVNITLANNTSPEGVKVQTLNDWETLLAKIDIKAAPLFMQLGLEDTE
                     ILKTLKQYTDKYNIELKTLQSGIGIDFIGELADRAYLPFSLEETWQKLEETVRWNIEN
                     ASANRIISIDASIYESAGASIVQELSFALSTAICYIKHLQDAGFTIEELAPLFQVKLS
                     LGSNFFMEIAKIRTFRLLWSEMIKAYGGEEEYQKVWIHGKTAKFNKSIYDIYVNVLRT
                     TTEGFAGVIGGVDSLEIGRFNELIAPADELSVRLARNQQIILKEEAHFDKVIDPAGGC
                     YYIEWLTNELANKSWALMQEIEAKGGMLEALINSDIHNYIESVAKQRIEAVNRRKSVF
                     IGVNMYANPLENMVSALPHSNSTCDEFTTTIKPLPKRRAVEDVENLRMQIEASNANKK
                     IFLLNMGTLAEYKARADFALNFFQVASFEVIYPNGFANIDEAIKEAEKSGAKAFCICS
                     TDDNYLNLVPEICSKLKGKIISLAGYPTDKIEDYKKAGINSFIHIKADIVTTLRELAK
                     QMEVIK"
     gene            complement(3009..4280)
                     /locus_tag="PKM80_03895"
     CDS             complement(3009..4280)
                     /locus_tag="PKM80_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNV93083.1"
                     /translation="MSKIANMLDADLENKICLVRMDHNVVKNGIIKDTMRIDATIPTL
                     MHIFRKGGLPILMSHIGRPFDKKTGTVNISEAESVVPVVKYLESKFQLRGLIPDCKPQ
                     DKIGITDYTPIGKAVESLKKGEVDFVYLPNTRWFKGEEAKDESTDVMAKSLAQFADVY
                     INDAFGSWQAHCSTYTIANYLPSYAGLLMQKEISNLQKIFEPKHPFLAIVAGAKFDTK
                     IGPLSSLIKIADKLILGGVIYNAYLCCKYNIKIEGVTNEDIEAANKFISESRDYIHKI
                     VELPLVIENTGMQKRENPKEIDIHILEPGTQLAYVLDVAGSNFAEECVKKVFQEAGTI
                     FVNAVMGYSALFPEGTMAMYSLIDQCQNAVKLFGGGDTIQDFKEYLPGIFAKAQNDPS
                     YYFFTGGGAILDAIQQGSPYGMKPVQALLRD"
     gene            complement(4615..4926)
                     /locus_tag="PKM80_03900"
     CDS             complement(4615..4926)
                     /locus_tag="PKM80_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNV93084.1"
                     /translation="MSNQPPQNQIQIKIDEKVGEGTYANFFVITNSPSEFIMDCGRIL
                     PGIPDARIYTRVLMTPSHAKQLMQLLEKNIDAYENQFGEIKLQGQQDNKPVGFKPDSK
                     I"
BASE COUNT         1506 a          972 c          827 g         1751 t
ORIGIN      
        1 aaaatcagtc ggtaaagcaa ttgcttcatc caaagaatta gtatgcaagg attgagtatg
       61 acccattgta gccgcaagtg cttcaataca agttcttgtt atattattgt aaggatcttg
      121 ttctgttaaa ctccatccgg aagtttgact atgtgttctt aaagccatag atttaggatt
      181 tttggggtta aactgcttaa tgatttttgc ccaaagaatt cttgctccac gcaattttgc
      241 cacttccata aaataattca ttccttctgc ccaaaagaag gaaagccgcg gagctataac
      301 atctatatct ataccagctt ttagagcagt acgcacatat tctaaaccat ctgctaaggt
      361 ataggccatt tcgaggtcag cagtagctcc tgcttcatgc atatgatatc cagaaatact
      421 tatactattg aacttaggca tcttttttga agtatagctg aatatatcgg ctattatccg
      481 cattgagggt tccggtggat agatgtatgt attacgcacc atatattctt tcaaaatatc
      541 gttttggatc gttccattta gagcttccgg tttcacacct tgttcttctg cggcaacaat
      601 ataaaatgcc attatcggaa taacagctcc gttcattgtc attgatacac tcattttatc
      661 cagtgggatt tgatcaaaga ggatctttat atctaaaata gaatcaatag ctactccagc
      721 ttttcctaca tctccaatca cgcgaggatg gtctgaatca taacctctat gagttggtaa
      781 atcgaatgct attgatagcc ctttttgtcc catagccaaa ttcctacggt agaaggcatt
      841 actttcttct gctgtagaaa aaccagcata ttgacgaatc gtccaaggtc gttgaatata
      901 cattgtagga taaggtcctc ttagaaaagg aggtaaaccg gatagataat ctaagtgttc
      961 gatattttcc atatcggctt taaaaaatag gggtttaact tctatctgtt cattagtttt
     1021 ccagctaaaa tcaagtttcg gttccgtttt ttgctcagcg ttaaaatcgg gcttaatctt
     1081 taagaaatca ggatttttca tttaatcacc tccatctgct ttgccaattc ccgtagggta
     1141 gttacgatat ctgctttgat gtgaataaaa ctatttattc ctgctttctt atagtcctca
     1201 attttatctg tgggatagcc tgctaagctg ataattttac cttttagttt tgaacaaatc
     1261 tcgggaacca aattgagata attatcatct gtagaacaga tacaaaaagc tttggcaccg
     1321 gatttttccg cttcttttat agcttcatct atgttggcaa agccattggg atagataact
     1381 tcaaaagaag ctacttgaaa gaaattaaga gcaaaatcag ctctggcttt atattcggca
     1441 agggttccca tattaagtaa aaagatcttt ttattagcgt tgcttgcttc aatctgcatt
     1501 ctaagatttt ccacatcttc tacagctctt cgctttggta atggtttaat tgttgttgta
     1561 aattcatcac aagtagaatt tgaatgaggc aatgcagaaa ccatattttc tagtggattg
     1621 gcatacatat taacgccaat aaaaacactt ttgcgtctat ttactgcctc aattctctgc
     1681 ttggctacag attcaatata attatgtata tctgaattta tcaacgcttc cagcattcca
     1741 ccctttgctt caatttcttg catcaaagcc caggatttat ttgccaattc attagtaagc
     1801 cactcaatat aataacagcc accagcagga tcaataacct tatcaaaatg agcttcttct
     1861 tttaaaataa tctgttgatt tctagcaagg cgaactgata attcatctgc aggagcaatt
     1921 aattcattga agcggccaat ttctaaacta tctacacctc caatcactcc tgcaaaacct
     1981 tcagttgtag ttcgtaacac atttacatat atatcatata tagatttatt gaactttgct
     2041 gttttgccat gaatccatac tttttgatat tcttcttccc caccgtatgc tttaatcatt
     2101 tcagaccaaa gtaaacggaa agtacgtatt ttagctattt ccataaagaa attggagcca
     2161 agcgaaagtt tcacttgaaa tagaggagcc agttcttcaa tggtaaaacc tgcatcctgt
     2221 aagtgtttta tataacaaat tgctgtagac aaagcaaaag acaactcttg aacaatggaa
     2281 gctccagctg attcataaat agaggcatca atactgatta tcctgttcgc ggatgcattt
     2341 tctatattcc atctgactgt ttcttctaat ttttgccagg tctcttcaag agaaaaaggc
     2401 agatatgctc tatctgccaa ttcaccgata aaatcaatac ctatgccaga ttgaagggtc
     2461 tttagttcaa tattgtattt atcggtatat tgttttaggg ttttcaggat ttctgtatcc
     2521 tctaaaccta attgcataaa caaaggagca gcttttatgt caatttttgc caacaaggtt
     2581 tcccaatcgt taagagtttg tactttcacc ccttcaggag aggtattatt tgctaaggta
     2641 atattaactg cggttaaacc ctgattcaat tctttcagta ttttctggtt taaagtgttt
     2701 agatcagaat ctgtatgatt ttgagcaatc aaccaaccct cagttaaaaa acggatagga
     2761 ttattccctc gcacataagg acttttacca ggtatacttt ccataaaagg aagtttagca
     2821 acatcttctt tacgataaat tggctgcaaa gttattcctt ccggggtttg ggttttcatt
     2881 actttttcaa aatccgcacc tttcaaacct tcatttacta cctgcaacca ttcctcatat
     2941 gttggaggag gaaattcttt cagcaggttc agtttattgt cattatccac tttattctcc
     3001 tataattatt aatctctaag taatgcttga accggtttca taccgtatgg agagccctgt
     3061 tgaatagcat ccagaatcgc gccccctcca gtaaagaaat agtagctggg atcgttttgt
     3121 gcttttgcaa aaattccagg aaggtattct ttaaaatcct gaattgtatc tcccccaccg
     3181 aaaagtttta cagcattttg gcattggtca atcaaagagt acatagccat tgttccctca
     3241 ggaaaaagag cactataacc catcactgca tttacaaaga tagttcctgc ttcctgaaaa
     3301 acttttttta cgcattcctc agcaaaatta ctacctgcta catctaagac atatgctaat
     3361 tgtgttcctg gttctaaaat atggatatct atctctttcg gattttcgcg cttttgcatt
     3421 cctgtatttt caattaccag cggtagttcc actatcttat gtatataatc tctactttca
     3481 ctaataaatt tgttggctgc ttcaatatct tcattagtta caccttcaat tttaatgtta
     3541 tatttacagc agagatatgc attataaata actccaccta aaataagttt atcagctatt
     3601 ttgatcaagg aagataaagg cccgattttt gtatcgaatt ttgctccagc cactattgct
     3661 aaaaaaggat gtttaggttc aaagatcttt tgtaaattgg atatttcttt ctgcatcaaa
     3721 agtccagcat aagaaggaag gtaattggca attgtataag tactgcaatg tgcttgccaa
     3781 gaaccgaaag catcattaat ataaacatca gcaaattgtg ctaaactttt tgccataaca
     3841 tcagtacttt catcttttgc ttcttcgcct ttaaaccagc gagtattagg aaggtataca
     3901 aaatccactt cacccttttt taaggattca acagcttttc caatcggagt ataatctgtt
     3961 atcccaattt tgtcttgggg cttgcaatca ggtattaaac ctctaagctg aaatttactt
     4021 tccaagtatt tcacaactgg aacaactgat tcagcttccg aaatgttgac agtaccggtt
     4081 tttttgtcaa agggtctacc aatatggctc atcaaaattg gtaaacctcc ctttctgaat
     4141 atgtgcataa gggttggtat tgtggcatca atacgcatag tatctttaat aatgccattt
     4201 ttaactacat tatgatccat acgcaccagg caaattttat tctccaaatc ggcatccagc
     4261 atattagcaa ttttgctcat ttccttatat ctccttttgt tatatattga tagttaagta
     4321 tttagattac tgtgatagca atatcatata ttaaagaatt tatctcataa ataaggatat
     4381 ttcgtcaagc taaatcttgg tcatcaaaca aggaagatag ataatagcaa acctatagcg
     4441 cttatcttgc ataggttatt ttagcaagat tatccaggtt aacagattaa caagtgaaag
     4501 gcaactatgc atttttttat aggtgttacc ttatcacctt tccataatat gagttctaaa
     4561 gttaatctgt taaccttttc tcttcaccct cttagctact gaccttttca ccctttatat
     4621 tttagaatcg ggcttgaaac caacaggttt attatcttgt tgaccttgta atttgatttc
     4681 gccaaactga ttttcgtatg catctatatt tttttccaaa agttgcatca gttgtttggc
     4741 atgagaagga gtcatcagaa cgcgtgtata aattctagca tcaggaatgc caggaaggat
     4801 acgaccacaa tccataataa attctgaagg ggaattggta ataacaaaga aattggcata
     4861 ggtgccttca ccgacctttt cgtcgatttt tatctgaatt tggttttggg gaggttgatt
     4921 agacatttta gatctccttt tttatgttat taaattaagt tatccttaat ttagtatagg
     4981 ttcttttgtc aattaaaaac agtattgagg ttgggtggta aaggtagaaa gtatcctctc
     5041 cttcagtttt aattcc
//
LOCUS       DAORHB010000101         4971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_17712_length_4971_cov_5.226200, whole genome shotgun sequence.
ACCESSION   DAORHB010000101 DAORHB010000000
VERSION     DAORHB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17712_length_4971_cov_5.226200"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1006
                     /locus_tag="PKM80_03905"
     CDS             14..1006
                     /locus_tag="PKM80_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV93085.1"
                     /translation="MVKIGVVGVGHLGQYHTQKYQNIDNAELVGVYDTNFPRAQEIAG
                     KLKVHCFSSYKEMLAVCDAVDIVATTTAHYELGKKALLSGKHIFVEKPITSELAQAQE
                     LLEIAAAKNLLIQVGHIERFNPVILKTENEISEPLFIESQRISSFQPRGTDVSVVLDL
                     MIHDIDLVLSFVQSPVSEIRASGVGIFTPTIDIATARIEFVNGAIANVTSSRVSMKQE
                     RTLRFFQRDAYVKMDFLSKQVRIIKKSDNVDKLLPLILSGVVDINPEQLLDIQNYDVG
                     DTEKDALTLELESFVDCVYNNKKPIVDGLAGTRALEIAIKITNQIQAQNAKLRA"
     gene            1022..2317
                     /gene="asnS"
                     /locus_tag="PKM80_03910"
     CDS             1022..2317
                     /gene="asnS"
                     /locus_tag="PKM80_03910"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNV93086.1"
                     /translation="MQDVFIKDIDKYIDKQVRLKGWVRNIRHSGKLLFIILRDGTGEL
                     QTVAFQPDLGDEKFELAKRLTLESSVIICGIPKPHPKKENVYELAVSDVMPIQIADEY
                     PISKKEHGPDFLLSNRHLWIRSQKQWAALRIRHSVYYGICNYLNEQNFIRFDSPILTP
                     NACEGTTTLFELEYFDEGKAYLSQSGQLYLETGIMSLGRVYDFGPVFRAERSKTRKHL
                     TEFWMMDAEAAYVEHEENMQIQEELIRYVIRYVLSNCKLELEILERNVEALKAADAPF
                     KRITHYEAVEFLRQKGSDITHNKDLGAQDEVLLTEDSTVPVFVERWPQEIKAFYMKRD
                     PENPNLVLGDDLIAPEGFGELIGGSQREDDYDLLYSRMVAEKMPIEDYQWYLDLRKYG
                     SVPHSGFGIGLERLVAWISGVPHIRETIPFPRMIYRIYP"
     gene            2464..3678
                     /locus_tag="PKM80_03915"
     CDS             2464..3678
                     /locus_tag="PKM80_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNV93087.1"
                     /translation="MITNYFSETTKTMKSSLIRELVAETKNVSGLISFAGGFPSPATF
                     PKDILSELYKEIVAQQGRDVLQYGNSEGDDILKDELLKWENYPNLNRNNMIITVGATN
                     AIYYMTRCLIDPGDVILCEAPTFLGTIVAFEAIGAEVQSIPMDNEGIDLNRMAEKVKQ
                     LRDANKNIKFLYTIPDFQNPAGITMSIQRRRDLLNFCEAEEILIMEDNPYSRLRFSGE
                     ELPTLFRIAHTEMNNSWLVTEIVSFSKILGPGIRVAYVKSHPELINKMVSWQQKVTIS
                     PDCVTERVVAKFIADGYMEPHIKSLCEFYKPYLDKMLTCLETEMPSYVQWTKPKGGIF
                     LWLWLPEDVNADTLFYKAKDHLVTFIPGSKFYPEGQEKFNCMRLNYTYSTFEQIEKGI
                     KHLGELVKSIYV"
     gene            3741..4187
                     /locus_tag="PKM80_03920"
     CDS             3741..4187
                     /locus_tag="PKM80_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93088.1"
                     /translation="MKKLILITTLSIFLLPLMAQIEFPRPNLNPYSSNGGNSLLNLEK
                     LSMSHSMGFEAGTSSTGYGYYLSRYTNHLKYNFNPKLELDLDLNFVNVGGFNTSSKFE
                     FNNDNSSRVIPEFSLSYKPRDNVQIQFQMLYGPYLNPYKDSWYRSW"
     gene            4222..4959
                     /locus_tag="PKM80_03925"
     CDS             4222..4959
                     /locus_tag="PKM80_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNV93089.1"
                     /translation="MAVLGAALGSFFNVLIYRIPKKQSIIFPGSHCESCKKAIPIYQN
                     IPIISYILLRGKCSRCGAKIHWHHLLVEIITPLLFLGLFFRYGLHDFRFYKYILLASF
                     LIPIFFIDAINQIIPHVLSIPLLISGIILAIIPGNDVGIVNALLTGVIVFSLLLLLAW
                     AYQKLRGVEGLGGGDIWLLTGLAIYFGLTGIPYIFLLSALMGIIYFLIFVKDKSKPFA
                     YGTFIAVAAIIWSVIGEDTIFNLLSFL"
BASE COUNT         1583 a          804 c         1002 g         1582 t
ORIGIN      
        1 aaaagcttaa gtaatggtta aaataggagt tgtgggagtt ggccatttag gtcaatatca
       61 tacccaaaaa tatcagaaca ttgataacgc agaattagtt ggtgtttatg acactaactt
      121 cccaagagcc caagaaattg ctggtaaatt aaaagtccat tgtttttcca gttataaaga
      181 aatgctcgct gtttgcgatg cagttgatat agtggctact acaacagcgc attatgaatt
      241 agggaaaaaa gcattacttt ctggaaagca tatttttgtg gaaaaaccga taaccagtga
      301 attagcgcag gcacaggaat tattagaaat tgctgcagct aaaaatctgt tgatccaagt
      361 gggtcatatt gagcgtttta atccggttat tttaaagacg gaaaacgaga tctccgagcc
      421 attgtttatt gagtcacaac gaatatcatc ttttcaacct cggggaacag atgtttctgt
      481 agtgctcgat ttaatgattc acgatatcga tttggtatta agttttgttc agagtcctgt
      541 atctgagatc agagcaagtg gagtgggtat ttttactcct acgattgata tagccactgc
      601 tcgcattgaa tttgttaatg gagccatagc aaatgttacc tcttctcggg tatcgatgaa
      661 acaggaacgc accttaagat ttttccagag agatgcttat gtgaaaatgg actttttatc
      721 caaacaggtt agaataatca aaaaaagcga taatgtagat aaattattac ctctaatctt
      781 gagtggagtt gttgatatca atccggaaca gttgttagat atacaaaatt acgatgttgg
      841 tgatactgaa aaagatgctc taactttgga actggaatct tttgttgatt gtgtttacaa
      901 taacaaaaag ccgatagttg atggcttagc gggaactaga gctttggaaa tagctattaa
      961 aataacaaat cagatacaag ctcaaaatgc caaattgaga gcttgaataa taggtagaaa
     1021 aatgcaagat gtatttataa aagatatcga taaatatata gataaacaag ttcggttaaa
     1081 aggatgggtt cgcaatatcc gtcatagcgg gaaactactt tttataattc ttcgagatgg
     1141 aactggcgaa cttcaaactg ttgctttcca acctgattta ggtgatgaga aatttgagtt
     1201 agcaaaacgg cttactttag agtcgtcggt aataatttgc ggcattccca aaccccatcc
     1261 taaaaaagag aatgtatatg aattagccgt aagtgatgta atgccgattc aaatagcgga
     1321 tgaatatcca attagtaaaa aagaacatgg tcctgatttt ctgctttcca atcgtcatct
     1381 atggataaga tcacagaagc aatgggctgc ccttagaatt cgtcattctg tttattacgg
     1441 tatttgtaac tacctgaatg agcaaaattt tattcgtttt gattcgccaa ttcttactcc
     1501 caacgcttgt gaaggaacaa ccactctttt tgaacttgaa tattttgatg aaggaaaggc
     1561 atatctttcc caatcgggtc agctttatct ggaaacagga attatgagtt tgggcagagt
     1621 ttatgatttt ggtcctgtct ttagagcaga acgctctaaa actcgtaaac atttaactga
     1681 attttggatg atggatgcgg aagcagctta tgttgaacat gaggaaaata tgcaaattca
     1741 ggaagagcta attcgctatg ttattcggta tgtgctctct aattgtaaat tggaattgga
     1801 gattttagaa agaaatgtgg aagctttaaa agcagctgat gcacccttta aaagaataac
     1861 acactatgag gcagttgaat tcttacgcca aaaaggtagc gatataaccc ataataaaga
     1921 tttgggtgct caagatgaag ttctgcttac agaggattca acagttcctg tttttgtgga
     1981 acgctggcca caagagatca aagcatttta tatgaaacgc gatcctgaaa atccaaacct
     2041 cgttttggga gatgatttaa ttgctcccga aggttttgga gaattgattg gaggcagtca
     2101 gcgcgaagat gattatgatt tattatattc gcgtatggta gctgaaaaaa tgcccattga
     2161 ggattatcag tggtatctgg atttgcgaaa atatggttct gtacctcata gcggtttcgg
     2221 aattggactg gaaagattag ttgcttggat aagtggtgtt cctcatatca gagagacaat
     2281 tcccttcccg agaatgattt acagaattta tccttaaaaa gtcttagctg gtagttggaa
     2341 gtttaacagc cggaaaagat caacacaaat gtactagcgg aatttgaatt ctaaagctta
     2401 ttgcaggtgt ttagtccata atcctaaatg attttgaata aaaaaaataa caaggataga
     2461 aaaatgatta cgaattactt ttctgaaaca acgaaaacga tgaaatcatc gctgatcaga
     2521 gaactggttg cggaaaccaa aaatgtttcc ggcttaattt cttttgcagg tggctttcct
     2581 tctccggcaa cttttcctaa agacatttta agtgaacttt acaaggaaat agttgctcag
     2641 caaggaagag atgttcttca atatggtaat agtgaaggtg atgacatttt gaaggatgaa
     2701 cttttaaaat gggaaaatta tcccaatttg aacagaaata atatgatcat cactgtagga
     2761 gctacaaatg cgatatatta tatgacccgc tgtttgatag atccaggtga tgtaattctt
     2821 tgtgaagccc ctactttttt aggaacaatt gtcgcttttg aagctattgg agcagaagtt
     2881 caaagtatcc caatggataa tgaaggaata gatttaaaca ggatggcaga aaaagttaag
     2941 caactaagag atgccaataa gaatatcaaa ttcctctaca ctatccctga ttttcaaaat
     3001 cccgctggaa tcacaatgag cattcagcgt cggcgtgatt tgctaaattt ctgtgaagca
     3061 gaagagattc taattatgga agataatccc tattcgcgtt tacgctttag tggagaagaa
     3121 cttcctaccc tatttcgtat tgcacataca gaaatgaata attcttggct ggtaaccgaa
     3181 atcgtttcat tttctaaaat cctaggacct ggaatcaggg tagcttatgt aaaaagtcac
     3241 ccagagttga ttaataaaat ggtctcctgg caacagaaag ttactatcag tccagattgt
     3301 gtaactgaac gggtcgtagc aaaattcatc gctgatggct atatggaacc acatattaaa
     3361 tctctgtgcg aattctataa accctatttg gataaaatgc ttacttgctt ggaaacagaa
     3421 atgccaagtt atgttcagtg gacaaaaccc aagggtggaa ttttcttgtg gttgtggttg
     3481 cctgaagatg taaatgccga tactcttttc tataaagcta aagatcactt ggtaaccttc
     3541 attccaggaa gcaaattcta tcccgaaggt caagaaaaat ttaactgtat gcggctcaac
     3601 tatacctatt ccacttttga acaaatcgaa aagggaatta aacatttagg tgagctagta
     3661 aaatcaattt atgtttgaca tcggtacagt tcttgcttat attattaagt gatatcttct
     3721 tttaagaagg ggaaataaaa atgaaaaagc tgatattgat tacaaccctg agcatttttc
     3781 tgctgccttt gatggcacag atagaatttc cgcgtcccaa tttgaatcct tattcaagta
     3841 atgggggaaa ttcgcttctc aatttagaga aactatcaat gagtcattca atgggctttg
     3901 aagcaggaac cagctctacc ggatatggct attatttatc ccgctataca aatcacctga
     3961 aatataactt taatcccaag ctggagctcg atttagacct taactttgta aatgtcggtg
     4021 gtttcaatac tagtagcaaa tttgaattta ataatgacaa tagtagtaga gttataccgg
     4081 aattttcatt aagttataaa ccacgagata atgtccaaat tcaattccag atgctttatg
     4141 ggccatattt aaatccctat aaagatagct ggtatagaag ttggtaagga gattcaactg
     4201 ttaacattcg taataataat tatagctgtc cttggtgctg ccttgggcag ctttttcaat
     4261 gtgctaattt accgcattcc taaaaagcaa tccattatat tccctggttc acattgtgaa
     4321 tcatgtaaga aagctattcc catctatcaa aacataccca ttatcagtta tattttgctg
     4381 agaggaaaat gttcaaggtg tggagctaaa attcattggc atcacttatt ggtagagatt
     4441 attacccctt tgttgttttt gggtttattc tttcgctatg gcttgcacga tttccgattt
     4501 tataagtata tattattggc ttcttttctg attccgatct ttttcattga tgcaataaat
     4561 cagattattc ctcatgtgct atccattcca ttgcttatat caggtattat tttagcaata
     4621 atccctggaa atgatgttgg cattgttaat gctttactaa ctggagtaat tgtcttcagt
     4681 ttattgctat tactggcttg ggcgtatcaa aaattacgcg gagtggaagg tttgggagga
     4741 ggagatattt ggttgttaac tggtttagca atatattttg gcttgaccgg tatcccttat
     4801 atctttttac tatctgcatt gatggggatt atctattttc tcatatttgt gaaggataag
     4861 agtaaaccat ttgcctatgg aacctttatt gctgttgctg caattatctg gtcagtgatt
     4921 ggagaagata caatttttaa cctattgagt tttttatagg ggctagccgc a
//
LOCUS       DAORHB010000102         4929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_17927_length_4929_cov_6.506155, whole genome shotgun sequence.
ACCESSION   DAORHB010000102 DAORHB010000000
VERSION     DAORHB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17927_length_4929_cov_6.506155"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..1138)
                     /locus_tag="PKM80_03930"
     CDS             complement(269..1138)
                     /locus_tag="PKM80_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNV93090.1"
                     /translation="MKAILTIAFVLFCICLSAVDYIDVVYTKNGDVLKGIIIENIPND
                     KIKVDINGVVVTVMYDQIIKLAKEEIDTTSTKVTKRNAISKTVNENDSDLPFHKKYGL
                     LGGVNSATAKVDNSEGVDISSVWGFSFGGFATFQTSPNIIIQPELIYTGKGFKTEINL
                     DDYDYEYKETDEWKFAYLEVPVLFKYVFQTGNMYITPFIGPSLSIAMSGKVSYDSYQY
                     YQNYEEEDSGSMDIKNQMEDIDFGLNFGTDLYFSEKIFLQLKYDLGLSKIFTKSVLES
                     KNRVLSLSMGVSF"
     gene            complement(1513..2865)
                     /locus_tag="PKM80_03935"
     CDS             complement(1513..2865)
                     /locus_tag="PKM80_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNV93091.1"
                     /translation="MLKYEYHNLLASKQMLSILPKDKIFDSEHQVKNIHEEIMAERRA
                     KLLGFYDLPENSTRHIDDFIANLDPVFDTMLVLGIGGSALGNKALYSALKTEAELPRK
                     LVVYDNVDPVFLYEILSQINLETTLVNVITKSGTTAETMAGYMIVADILKRKFPKDYQ
                     KRIIVTTDKERGFLRQVIKKEGYKSFTVPDNVGGRFSVLSDVGLVSSAFTGINIQELL
                     SGAKAMRERCSEPDIMENPAYLNGLIHFLYYRLGKNISVMMPYSNSLYDLADWYRQLW
                     AESLGKRKDIKGREVYVGQTPVKALGTTDQHSQVQLYTEGPNDKVITFITVENFKHDY
                     IIPNLHPDHEEVNYLGNRKLSDLLNAERLATEIALSKAMRPNANIVFPQIDEYHLGEF
                     IMLYEIQTILTGKLLRINPLDQPGVESGKIATYALMNKKGYEKEHAEIEKYKQDRDIT
                     "
     gene            complement(2866..4599)
                     /gene="ptsP"
                     /locus_tag="PKM80_03940"
     CDS             complement(2866..4599)
                     /gene="ptsP"
                     /locus_tag="PKM80_03940"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNV93092.1"
                     /translation="MQKIYGTSLNTGIFIGNALHIALPEPAIETGNIRQEEIPKEISR
                     LQSSLSIVEEDINLELKTSNLQAQDAEIISAQREILRDPEIYNQIETAIRNKLQYATQ
                     AVSECFANIIKHFETMKIETFAVRAADYRDVAMRLLNALSGVRNTLKIKIEPNQIPVF
                     IEIVPSLVSKLAQEGIKAYLVAKGSYNSHSSILCRALGLVAIANIPDLNVAVKNNDLL
                     IVDGYTGLLIVNPDDQTLEYYRKKAEAERHRQEKLKQLKITDSITANGINIELFTNIG
                     VPEELEVIKNLSCAGIGLFRTEFLYLGRQNLPDEEEQFAVYKELAEKMKPLPVTIRTF
                     DLGGDKLSPLGYETPEENPYLGNRGIRFSLSQPDIFYTQVRAILRASAFGKVQIMFPM
                     IIDTQDFIEAKKFVQNCQRELSQHSISFDPEIALGAMIEIPSAALCSEELAKECDFFS
                     IGTNDLVQYTLAADRNNEMVAKYYIQHHPAVLCLIKKTIISAEKYHIPLSVCGEMASN
                     YKYIPLLIGLGIRELSVNPGSFFSAKAIVQKCDAKLFKATEEINCSTPLSEVENLINN
                     ILAPYYQDKEE"
     gene            complement(4600..4863)
                     /locus_tag="PKM80_03945"
     CDS             complement(4600..4863)
                     /locus_tag="PKM80_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNV93093.1"
                     /translation="MKQIEVTVTNKLGLHARPSALLVKAATKYRSDFFIEKEGMRING
                     KSIMGVMMLAAECGSKIKLIADGVDEDYLLEEIKTMIESGFGE"
BASE COUNT         1455 a          969 c          801 g         1704 t
ORIGIN      
        1 tgcctctgct tctcgttgca aattcaagaa ggatcggttt agagtcaaag acacacaaca
       61 ctaaaccgag cttcagcttt gtaagttgaa ggcaaagaac aaattttcct gttgcctctg
      121 cttctcgttg caaattcaag aaggatcggt ttagagtcaa agacacaatt ctttactgaa
      181 atgtatgtat tactatatca agcattctta tagcctaaaa catcgatttc ttcatctctt
      241 tctattaatg aacttatatc tgtatttttt agaaagaaac gcccatactt aaactcaaga
      301 ccctattttt tgattctaaa acacttttag taaaaatctt tgaaagacct aaatcatact
      361 tgagttgcaa aaaaatcttt tcggagaaat acaggtcagt tccaaaattc agtccgaaat
      421 caatatcttc catctgattt ttaatgtcca tagaacccga atcttcttct tcataatttt
      481 gataatattg atacgaatca taagaaactt tcccactcat tgcgatacta agtgatggtc
      541 caataaaagg agtgatatac atatttcctg tttggaagac atatttgaac aatacaggaa
      601 cttccaaata ggcaaatttc cattcgtcgg tttctttata ttcgtaatca taatcatcta
      661 aattaatttc tgttttaaac ccttttccag tatagattaa ttcaggttga atgattatat
      721 tcggactcgt ctggaaggta gcaaaacctc caaaagaaaa tccccatact gaggatatat
      781 caacaccctc ggaattatca actttagcgg ttgctgaatt cacaccccca agtaaaccgt
      841 attttttgtg aaagggtaga tctgagtcat tttcattaac agttttactt atcgcatttc
      901 tttttgtgac ttttgtagat gtcgtatcaa tttcttcttt tgcaagtttt atgatttgat
      961 cgtacataac agttactaca acaccattga tatcaacttt gatcttgtca ttgggaatgt
     1021 tctcaataat gatacctttt aacacatctc catttttagt gtagaccaca tcgatgtaat
     1081 ccactgctga gaggcaaatg caaaacagta caaacgctat tgttaggata gctttcactt
     1141 ataactcctt tatagtataa atattaattt gtccttgttt tctcttctat gaattcggtc
     1201 aagttttttc taactaggga tccctctgca aaattaattt agttgattcc caagtatcag
     1261 tgtaaattcc aactcgttat ttttcaagtt gttttaagaa cattctcatt atcctcagtt
     1321 cttaagttct acattgtgtc ttacgacaat tgcattattt tcattttgcc aattcctatc
     1381 aatattattt tcataatcat agtttttgct tttaaaatca tatccgcctc cataacaggg
     1441 tagcttttta ccctcttatt caagattcta tttttcatct cttttttgta aatcaatgaa
     1501 ctaatatacc tattaggtta tgtcacggtc ttgtttgtat ttttcaattt cggcatgttc
     1561 tttctcgtag ccctttttgt tcatcaaggc gtaggtagca atttttccag attccacacc
     1621 gggttgatct aatggattaa tacgaagaag tttacctgtt aatatcgtct gaatttcata
     1681 aagcataatg aattctccta aatggtattc atcaatttgg gggaaaacaa tattagcatt
     1741 gggacgcata gctttactga gggcaatttc tgtagcaagt cgttctgcat taagcaaatc
     1801 ggagagtttt ctattgccca gataatttac ttcttcgtgg tctggatgca aatttggaat
     1861 tatataatca tgtttaaagt tttctacggt tatgaaagtt attaccttgt cattaggtcc
     1921 ctctgtataa agttgcactt gcgaatgttg atcggttgtt cctaatgctt tcacaggtgt
     1981 ttgccccaca tatacttctc taccttttat gtctttgcgt ttacccaaac tttctgccca
     2041 aagttgacga taccaatcag caagatcata caaagaatta ctataaggca tcattacact
     2101 gatgtttttt cccaagcgat aatataagaa atgaatcaaa ccattcaagt aagctggatt
     2161 ctccataata tctggttccg aacagcgttc cctcatagct tttgctccac ttagcaattc
     2221 ctgaatatta atgccagtaa aagcagatga aactaaaccc acatcggaaa ggacactaaa
     2281 tctgcctccc acattatctg gaacggtaaa gcttttatag ccttcttttt taataacctg
     2341 gcgcaaaaag cctctttctt tatctgtagt aacgataatt ctcttttgat agtctttggg
     2401 gaacttgcgt tttaatatat cagccacaat catatagcca gccattgttt cagcagttgt
     2461 tccgcttttg gtgatcacat ttaccaaagt tgtttccagg tttatttgcg acaagatttc
     2521 atataagaaa acaggatcca cattatcata aacaacaagc ttacgaggta attctgcctc
     2581 cgttttcaaa gctgaatata gtgctttatt acctaaagcg gaaccgccaa tgcctaaaac
     2641 taacatagta tcaaaaaccg gatccaaatt agctataaag tcatcaatat gtctggtaga
     2701 attttccggt aaatcataaa aaccaagtaa cttagcccgg cgttcagcca taatttcttc
     2761 atggatattt ttaacctgat gttcgctatc gaaaatttta tctttgggca aaatactcag
     2821 catctgtttg gaagctaata gattgtgata ttcatatttc agcacttatt cctccttgtc
     2881 ctgataatat ggagctaaaa tattatttat taagttctct acttcggaaa gcggagtgga
     2941 acaattaatt tcttcagtcg ctttaaaaag ttttgcatcg catttctgta caatggcttt
     3001 tgcggaaaag aagctaccag gattaacgct taattctcta attcccaaac caattaaaag
     3061 gggaatatac ttatagttgg aagccatttc tccgcaaaca gaaagcggaa tatgatattt
     3121 ttctgcacta attatagttt tctttatcag gcaaagcaca gcaggatggt gttgaatata
     3181 atatttggcc accatttcat tattacgatc tgcagccaaa gtatattgta caaggtcgtt
     3241 tgttccgata ctgaaaaaat cgcactcttt ggctaattct tcagaacata atgctgcgga
     3301 gggtatttct atcatagccc ccaaggcaat ttctggatca aaggatatac tatgttggct
     3361 aagttcccgc tggcaatttt gcacgaattt ctttgcttcg ataaaatctt gggtatcaat
     3421 gatcatagga aacataattt gcacttttcc aaaagcggaa gcccttaaga tagcacgcac
     3481 ttgagtataa aaaatatctg gttgggaaag tgaaaatcgg attccgcgat tacccagata
     3541 gggattttct tccggagttt cataacccaa gggggagagt ttatctcctc ccaaatcaaa
     3601 ggttcggatg gtaacaggca aaggtttcat tttttcagca agttctttat atacagcaaa
     3661 ttgctcttct tcatccggaa gattttgcct tcccagatag agaaattcag ttctaaacaa
     3721 accaatacca gcacaggaaa ggttttttat cacttccaat tcttctggta cgcctatatt
     3781 agtaaacagc tcaatattaa tcccattagc ggttatggaa tcagttatct tcagctgttt
     3841 cagtttttcc tgtctatgcc gttctgcttc agcttttttt ctgtaatatt ctaaggtttg
     3901 gtcatcggga ttaacgatca gcaatccagt atagccatca acaatcagca agtcgttatt
     3961 tttcactgcc acatttaaat ccggaatatt agcaatagcc accaatccca gagcccggca
     4021 taaaatagaa gaatgtgaat tgtagcttcc tttggccacc aaatatgcct taattccttc
     4081 ttgggcaagt ttactaacta aagaagggac aatttcaata aaaacaggga tctgatttgg
     4141 ttcaattttg atttttagag tatttcgaac tcctgatagg gcatttaata ggcgcatagc
     4201 cacatcccga taatcagcag cgcgaacagc aaaggtttct attttcatag tttcaaagtg
     4261 tttaataata ttggcaaagc actcagaaac cgcttgggta gcatattgta atttattgcg
     4321 aatggctgtt tcgatttgat tatagatttc cggatcgcgt aaaatctctc tttgagcaga
     4381 gatgatttca gcatcctgag cttgcaggtt gcttgttttt aattccagat tgatatcttc
     4441 ttccacaata gaaagagatg attgcaaccg ggatatttct tttgggattt cttcctgcct
     4501 gatatttcct gtttcaatag ccggttcggg caaagcaata tgcaatgcgt tgccaataaa
     4561 aataccggta ttcaaggatg ttccatatat tttttgcatt tattctccaa atccgctttc
     4621 gatcattgtt ttgatttctt ccagcagata atcctcatca actccatcag cgataagttt
     4681 tattttagaa ccgcattcag cagccagcat cataactccc ataatgcttt taccatttat
     4741 tctcattcct tctttttcaa taaaaaaatc gctgcgatat tttgtagcag ctttaactaa
     4801 gagagctgag ggtctggcat gcaaacccaa tttattggtt acggtcacct ctatctgttt
     4861 catcactttt gccttcaatg tttttcagca ttgttttctt acggatttct tcgtttacct
     4921 tgcggttaa
//
LOCUS       DAORHB010000103         4919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_17980_length_4919_cov_3.998561, whole genome shotgun sequence.
ACCESSION   DAORHB010000103 DAORHB010000000
VERSION     DAORHB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17980_length_4919_cov_3.998561"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1030)
                     /gene="mutM"
                     /locus_tag="PKM80_03950"
     CDS             complement(218..1030)
                     /gene="mutM"
                     /locus_tag="PKM80_03950"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNV93094.1"
                     /translation="MPELPEVETIVKGLKKVLKGKVINSLECYYPGTIIYDTDIEATP
                     FPAKVLELCRRGKYIIITLETDNAIIIHLRMTGKLVFDVLPSEPLQYERARIILHNSE
                     VLHFIDIRTFGKIIICKQQNEEQYLPILGIEPFSKNFNANHLKAILKGKEASIKTVLL
                     NQSIIAGLGNIYTCEILYRAGINPLMPAGKLSHKEIVEIVQQTLAVLSEAISLGGTSI
                     SDYRNIDDKTGSFQDFLKVYQKQFCPLGHKIERIKQSGRSTFFCPICQKNKS"
     gene            complement(1115..3643)
                     /locus_tag="PKM80_03955"
     CDS             complement(1115..3643)
                     /locus_tag="PKM80_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNV93095.1"
                     /translation="MIDNEGTMKKAMSILVLLLIVTILSALPKELTRSGNPELYKEFS
                     KKARVNLKQLQYKQKEAYQKMLKEHPDVLMCYLIAYECDANLAAADPEDIESNYQTIC
                     ALVNKEGLKYKPEFFLSYIAKQTVSDEAITAYRKAMLKDGLQNIINTTHNDIDRFRAV
                     TLWCVSRLQFQQTSGRDQTPLDIIHKSIIGRCEEMQILLVAAARTVGLPSRPASVPWW
                     AHIDNNHAWTEIFLDGEWHYTGDMDMAYFPDQTWFSGLVDKTVMILAEGSLATWDDEV
                     LAKGKYETLINSTRNYTKERSRTIKLKVINENGKPVTNAYVIPMVFNWGSLRALTVLT
                     TNKNGYLQFTAGRGAFYLSVYSKDGKALILVPSNEKTNVELTAKLDNNDFPGGWASLE
                     YPGNIHNWQTQPEEYTLAVNLEKQLWQEKQKAFETNAIKAKVKDDSLMIKVAIACRGN
                     FTAFWDFVKRNPTPEASFLEFLLLGDPKFLWQAKAEQFEALYENWIRLQDPDIDEENT
                     LSLFSSSVFYEDLPEPIEYVKGKAQLYPKAFRFNYPHNREGAIKVLSKLKQKYKIKPE
                     KALSGLPPLHIAIEHKYLTPVQYRIMAVYILRANGFPAEFSRIPNLVAVFIDGDWQYV
                     NIVKLSWEDRSKDDKASTFHCTLQINDTDNNPIKVEEEQLTLCRYENGMFYPLNVNFD
                     YLGSGKYTGIFPVYETWLQFGYRVSDSKTKFYYQQIKPNIDDVIHLEIIAEEYPRKWE
                     AADSELIALLDDIQNSENKIILIGNYDQENSRRLADKLISLKKDFVWLGYSTSETAPQ
                     NYILSNKWNSMVRENTHNAMRSITLIKIGETWQMYDGLWDKLPD"
     gene            complement(3743..>4919)
                     /locus_tag="PKM80_03960"
     CDS             complement(3743..>4919)
                     /locus_tag="PKM80_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNV93096.1"
                     /translation="LLCAISEMAFKEGENFGALSFAEIQGYLYSLLDEGLIKHISDRY
                     IGISNSYPAADVSLRNAGNQFQILADNELVGWVDSGSVKWMTHPEAIYLHQGETWIVK
                     ELDFEQKKVILEPIQVNYYTQAIQYTEIGLDDLLKLENVTGGRKHFGKVTVSRTITGF
                     KKLRFYTLEVLEQEELDLPPEVMQTQAYWISLSGETVEKIRNQGLWANDKNDYGPQWE
                     KLTEKIRQRENYRCRNCGVTGDLEVHHIIPFRRFDDPDEANDPDNLVALCPRCHKLAE
                     TRVHIQSGLAALAYLLGNLAPFFVMCAPQDLGIHSEDKSPLALGNPVIAIYDNIPGGI
                     GLSGKLYELHNQLLYAAIDRVQGCSCENGCPSCVGPVAENGLGAKEEAIAILKELIS"
BASE COUNT         1472 a          989 c          781 g         1677 t
ORIGIN      
        1 ataatcagat aagcattata agtgcttccc cgttgtgtta cataaccatg aaaattacgc
       61 agatcccaat ctatagctcc aacccaatat ataccttctt tcagttttag tgctttcact
      121 atgcttacat ctcctttatg aattatattt gcagatttcc tataagcagt tctaactttt
      181 ctacataatt acataatata tcgtttacct taactagtca acttttattt ttttggcata
      241 ttgggcagaa gaaagtggag cgtcctgatt gttttatgcg ctctatcttg tgccctaagg
      301 gacagaattg cttttgatat accttcaaga aatcttgaaa gctgcctgtt ttatcatcta
      361 tattacgata atctgagata cttgttcctc ccaaggatat tgcttcagaa agaactgcta
      421 atgtttgttg aactatttcc acaatttctt tatggctaag tttccctgct ggcataaggg
      481 gatttattcc tgctcgataa agaatttcac aggtataaat atttcctaat ccagcaataa
      541 tactctgatt aagcaaaacg gtttttattg aagcttcctt acccttcaat attgctttta
      601 aatggttagc gttgaagttt ttactaaaag gttcaattcc caaaataggt aaatactgtt
      661 cttcattctg ctgcttgcaa atgattattt tgccgaatgt ccgtatatcg atgaaatgca
      721 aaacttcact attgtgcaaa attatcctgg cgcgttcgta ttgtagaggt tcacttggaa
      781 gaacatcaaa aaccagttta ccagtcatcc tgagatgaat gataatggca ttatctgttt
      841 ccagagtgat aatgatatat ttacctctgc gacaaagctc caacaccttt gctggaaaag
      901 gtgttgcctc tatatcagta tcataaatta tagtccctgg atagtaacat tcaagagagt
      961 ttattacttt ccctttcaga acttttttca agcccttaac gatggtctca acttcaggca
     1021 attcgggcat ttaatttgtc ctttcttatt aatgtctttt ttatccgtga aattttagta
     1081 gtctgttagc gacactaatc tataataatc atttttaatc gggaagttta tcccacaatc
     1141 catcatacat ttgccaggtc tcaccaattt ttattaaagt tatgctacgc atagcattat
     1201 gcgtgttttc gcgtaccatc gaattccatt tgttacttag aatataattt tggggagctg
     1261 tttctgaagt actataacct aaccaaacaa aatctttttt caaggaaatt agcttatcag
     1321 ccaaacgccg gctattttct tggtcgtagt ttccaatcaa gataatctta ttttccgagt
     1381 tttggatatc atcaagcagg gcaattagtt cactatcagc tgcttcccat tttcggggat
     1441 attcttctgc tataatttcc aagtgaataa catcatcaat attaggtttt atctgttgat
     1501 aatagaactt tgttttgctg tctgaaaccc tatatccaaa ctgtagccag gtttcataaa
     1561 cgggaaatat tcctgtatat ttcccagagc caagataatc aaaattaaca tttagcgggt
     1621 aaaacattcc attctcataa cggcaaagag taagctgctc ctcttccacc ttaataggat
     1681 tattatctgt atcattaatc tgtaaagtac agtggaaagt actggcttta tcatctttgg
     1741 aacgatcttc ccaagaaagc ttaacaatgt ttacatattg ccaatcacca tcaataaaaa
     1801 ccgctaccaa atttggaatt cgagaaaact cagcaggaaa accattagct cgtaatatat
     1861 aaacagccat tattctatat tggacaggag taagatattt atgctctatg gctatatgta
     1921 aagggggcaa tccacttaga gctttttctg gtttgatttt atatttttgt ttcagttttg
     1981 atagaacttt gatagcccct tcccgattgt gggggtaatt aaatctaaag gctttgggat
     2041 aaagttgtgc cttgcctttt acatattcaa tcggttctgg taagtcttca taaaaaacac
     2101 tggaagaaaa taagcttagg gtattctctt catctatatc gggatcttgt aaccggatcc
     2161 agttctcgta tagagcttca aattgttccg ctttcgcctg ccacaaaaat ttcggatcgc
     2221 ctaataataa aaattctaaa aagcttgctt ccggagtagg attgcgtttt acaaaatccc
     2281 aaaaagcagt aaaattacct cgacaggcaa tagcaacctt tatcataagc gaatcatcct
     2341 ttacttttgc ttttatagca ttagtttcaa atgccttttg cttttcctgc cagagttgtt
     2401 tttccagatt taccgctaag gtatattctt caggttgagt ttgccaatta tggatattac
     2461 ccggatattc caaagatgcc cacccccctg ggaagtcgtt attatcgagc ttagctgtca
     2521 attcaacatt tgttttctcg ttggaaggga ctaaaattaa tgccttacca tctttgctat
     2581 aaacagataa ataaaaagca ccacgaccag cagtgaattg taaatagcca tttttatttg
     2641 tagttaaaac agttaaagct cttaaagatc cccaattaaa aaccattgga atcacataag
     2701 cattagtaac aggtttacca ttttcgttta ttacttttag ttttatggtt ctacttcgct
     2761 ctttggtata attacgcgta gaatttatca gggtttcata ttttccttta gctaagacct
     2821 catcatccca agtagctaaa gaaccttctg ccaaaatcat aacagtttta tcaactaaac
     2881 cactaaacca ggtttgatca ggaaaatatg ccatatccat atctcctgta taatgccatt
     2941 caccatccaa aaagatttcc gtccaggcat gattattatc aatatgagcc caccaaggaa
     3001 ctgaagctgg acgagacggt aacccaactg ttctggccgc agcaactaac aaaatttgca
     3061 tttcttcaca gcgcccaata atcgacttgt gaattatatc caagggcgtt tgatcccttc
     3121 ctgaagtttg ctgaaattgc aagcgggaaa cacaccataa agttacagcc ctgaatctgt
     3181 ctatatcatt atgggtagta ttaataatat tctgtaaacc atcctttagc attgcttttc
     3241 tgtatgcagt aatggcttca tcagaaactg tctgtttcgc tatgtaagaa agaaaaaact
     3301 caggtttgta tttcaaccct tctttattta ctagagcaca gattgtttga tagttacttt
     3361 cgatgtcctc tggatcggca gctgccaaat tggcatcaca ttcataagca ataagatagc
     3421 acattaaaac atctggatgt tcttttaaca ttttttggta tgcttccttt tgcttatatt
     3481 gcagctgttt taagtttact cttgcttttt tagaaaattc tttatagagc tcaggatttc
     3541 cggatcgggt aagttctttt ggtaacgcag agagtattgt tactattagc aaaagaacaa
     3601 ggattgacat tgcttttttc atagttcctt cattatctat catttttttg ttacattggc
     3661 gattttatct atattataat tgcctataga gcatatagca aattgaatta tatcccgatt
     3721 atgaattttg ctgctccctc atttatgaaa tcagttcctt taaaattgct attgcttctt
     3781 cttttgctcc caaaccattt tctgctactg gtcctacaca agagggacaa ccattctcac
     3841 aactacaacc ttgcactcta tctatggctg catatagtaa ttgattatgc aattcatata
     3901 gttttccgct aagccctata cctccgggaa tattatcata aatagctatt acaggatttc
     3961 ctaatgccaa aggggacttg tcttcactat gaattcccaa atcttgagga gcacacatca
     4021 caaaaaaagg tgctaaatta ccaagcaaat atgctaaagc agctaaaccg ctttggatat
     4081 gaactcttgt ttcagctaat ttatgacaac ggggacaaag agctacaagg ttatcaggat
     4141 cattagcttc atctgggtcg tcaaaacgac gaaaaggaat tatatgatgg acttccaaat
     4201 ctcctgtaac accgcaattt ctgcagcggt aattttccct ttggcgtatt ttttctgtta
     4261 gcttttccca ttgaggtcca taatcatttt tatcattagc ccaaagaccc tggttacgaa
     4321 ttttttctac tgtttcccct gaaagagaaa tccaatatgc ttgggtttgc ataacttcgg
     4381 gaggtaaatc aagttcttcc tgctctaaaa cttccaatgt ataaaaacgc agttttttaa
     4441 aacctgttat tgtgcgggaa acagttactt ttccaaaatg tttacgacca cccgtaacat
     4501 tttccagctt cagcaaatca tccaaaccta tttctgtata ttgaattgct tgagtgtaat
     4561 aattaacctg aatcggctct aaaatgacct ttttttgttc aaaatccagc tctttaacaa
     4621 tccaggtttc accctggtga agataaattg cttctggatg agtcatccat tttacacttc
     4681 cagaatcaac ccaaccgact aattcattat ctgccaagat ttgaaactgg tttcctgcat
     4741 tgcgtagaga aacatctgca gcaggataac tattactaat tccaatatag cgatcactaa
     4801 tatgctttat taaaccttca tctaaaagag aataaagata accctgaatt tcagcaaaac
     4861 ttaaagcacc aaaattttca ccttctttga acgccatttc actaatcgca catagcagc
//
LOCUS       DAORHB010000104         4912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_18016_length_4912_cov_4.408483, whole genome shotgun sequence.
ACCESSION   DAORHB010000104 DAORHB010000000
VERSION     DAORHB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18016_length_4912_cov_4.408483"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PKM80_03965"
     CDS             complement(<1..949)
                     /locus_tag="PKM80_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93097.1"
                     /translation="MKKYYFILITALVFLGACEVKKPTMPSWSVKLNIPLINDFFYVS
                     DLVDSVNIVLNEDNILTVTANGEATTNEFGNLPFNPNINLTGIPLIGSSYDFYIPFTD
                     PTGTVEIAYAELAEGVFESRLRNVDPNVDQISLVFHDIYLPNSNQNLVIISDNFDNWV
                     TTNLDGMHLGTPNTNEIITQLHISIQVNSTLPAGTQVATLDFRSDSMISLGEFRGGLH
                     NYPVNLNDSDSSINIDYPHGLDQTLTLEEASLQLTLQNYIGFGAEFSGQLKATNAAGV
                     SRIIDILDENGNNFQIQPALQDGPVVMNFNLYDNITELLQ"
     gene            complement(1470..2249)
                     /gene="rnc"
                     /locus_tag="PKM80_03970"
     CDS             complement(1470..2249)
                     /gene="rnc"
                     /locus_tag="PKM80_03970"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNV93098.1"
                     /translation="MEKRTKSIISRIVEYFNTKKIGEKYPKWEKSLTQLQKKIDYTFH
                     DPTLLHAALTHKSYLHRHYSEHKTPSPFERMEFLGDSILGFIVSKELFTEHPEEQEGK
                     LSKLKSKIVSETYLTFKANAIDIGKYVLLSPEEQQAGGASKPSILSDTMEALICAIYL
                     DSGISAASKFIRRFILDNYQETVNREELVNYKSILQEYMQSRNQGPPNYVTIAEEGPE
                     HHKIFVVEVRWGDKLLGLGKGSTKKSAHQEAARMACQKLGI"
     gene            complement(2273..3514)
                     /gene="fabF"
                     /locus_tag="PKM80_03975"
     CDS             complement(2273..3514)
                     /gene="fabF"
                     /locus_tag="PKM80_03975"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNV93099.1"
                     /translation="MNKRRVVITGLGAITPVGHNVSQTWQSLINGVSGVGLISRFDID
                     NLPVHIAAEVKNYNPEDHFEFKEIKKLDLYAQFAMIAAREAIADAGLTPNSYDPNRVG
                     VITGAGIGGILTFEEECIKNYTMGPRRVSPFFIPKMIGNIAAAHISIENNFKGINFNV
                     MSACASANHAIGTAYRAIQYGDADIILSGGNEAAVTPLSIAGFSAMRALSTRNDDPEK
                     ASRPFDAERDGFIMSEGAAILVLEELEHAIARGAKIYAELIGYGASEDAFHITAPTEN
                     GEGSARAMLMAINDAGIKPTDIDYINAHGTSTPLNDKGETHSIKTVFGDYAYKVKINS
                     TKSMVGHMLGAAAGIEAIVCVKTIENGIIHPTINLTHPDPECDLDYTPNVAIHHQVDI
                     TLSNSLGFGGHNSAIIIKRYQ"
     gene            complement(3680..3916)
                     /locus_tag="PKM80_03980"
     CDS             complement(3680..3916)
                     /locus_tag="PKM80_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNV93100.1"
                     /translation="MMDIEEKVKQIVAKKLGVPESQVVPSANFIDDLRADSLDTVELV
                     MAFEEEFDLTIPEEDQDKLKTVGQAIDYLKEKLG"
     gene            complement(3962..4711)
                     /gene="fabG"
                     /locus_tag="PKM80_03985"
     CDS             complement(3962..4711)
                     /gene="fabG"
                     /locus_tag="PKM80_03985"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNV93101.1"
                     /translation="MKLDFTDKVIVITGAARGIGFAIAKTFANANAKVIIIDVASEAV
                     EKAIAELQENGFQAYGYVGNVTDAKSIETIFADVVDKYGTIDCLINNAGITRDNLLIR
                     MKEEDWQLVMDINLKGSFLCTQKAFKYMMRSRNGCIINIASVIGIMGNSGQANYAASK
                     GGLIAFTKSCAKEFGSRNIRVNAVAPGFIETEMTASLSSETRAEYAKAIPLQKMGTPM
                     DIANVCLFLASDAGSYLTGQTIAVDGGLTMH"
BASE COUNT         1527 a          968 c          813 g         1604 t
ORIGIN      
        1 gctgtaagag ttctgtaata ttatcatata gattaaaatt cataaccaca gggccatctt
       61 gcaatgctgg ttgtatctga aaattgtttc cattttcatc caaaatatca attatgcgag
      121 aaacacctgc agcattagta gcttttaatt gtcctgaaaa ttctgctcca aaacctatat
      181 aattctgcaa agtaagctga agtgaagctt cttccagagt taaagtttga tctaagccat
      241 gaggataatc aatattaatt gaagaatccg aatcattcaa attaacggga taattgtgta
      301 aaccacctct aaattctccc aaactaatca ttgaatctga tctaaaatct aaagtagcta
      361 cttgtgtccc agcaggaaga gtagaattca cttgtataga gatatgtaac tgggttatta
      421 tttcgttagt attcggcgtt cccaaatgca ttccatccaa atttgtcgta acccaattat
      481 cgaagttatc gctaataatt accaagttct gattgctatt gggcagataa atatcgtgaa
      541 aaaccaaaga aatttgatca acatttggat caacatttct caggcgactt tcaaaaacac
      601 cttctgcaag ctcagcatat gcaatctcaa ctgtaccagt gggatctgta aaaggaatat
      661 aaaaatcata actcgagcca attagaggaa ttccagttaa attaatatta ggattaaaag
      721 gtaaattgcc aaattcgttt gttgttgcct caccattagc tgtaactgtt aagatattat
      781 cttcattcaa aacgatgttt acagaatcta ccaaatcaga cacataaaaa aaatcgttga
      841 tgaggggaat atttaattta acactccagc ttggcattgt gggttttttc acctcgcaag
      901 caccaagaaa tactaaagcg gtaatcagaa taaaataata ctttttcata agaaaatcct
      961 tttgctgctt aagttctatt aagagctctt ctcatagaga tcgaggaaat aacttctccc
     1021 caaaccaata tagacattat aacattcttg aaaaacaaaa atcacacgat taattcctac
     1081 cgatgataat gataatgtag ttaaagacaa aataaacaat tgctgccttt tggcaagtta
     1141 tttctaagaa gattgaaaac taaagcagct ccaataataa taatgtgttt aacaaaactc
     1201 taagaaggca atagaataat attacttccg aaatgagaaa ggcgtgcagc atacaaaaag
     1261 agtatataat aggtttgctt atatttagtt ggaactgcca atttgtgcaa tccaattaac
     1321 cgtaaactgc attattttct agtacctggc ttacgggttc ttctcttgca gttttattac
     1381 gattaactac actaaaaaac aataggatta gcgttcaact aagggtacaa tatgggttcg
     1441 ccatctctcg ctgtatatgt tttcctttat tatataccaa gtttctggca ggccattctt
     1501 gcggcttcct gatgtgcact ttttttggtg cttccctttc ccaggcctaa aagtttatcg
     1561 ccccaacgaa cttccacaac aaaaatctta tggtgctcgg gtccttcttc ggcgatagta
     1621 acataatttg gtggtccttg atttctgctt tgcatatatt cttgcaaaat acttttgtaa
     1681 tttaccagct cttcgcggtt aacggtttct tgataattat ccagaataaa acgacggata
     1741 aatttgctgg ctgcagatat tccactatcc agataaatag cacaaattag agcttccatt
     1801 gtatccgaca ggatagaagg tttggaagct ccccctgctt gttgttcttc tggacttaaa
     1861 agtacatatt taccgatgtc tatcgcattt gctttgaaag ttaagtaagt ttcggaaaca
     1921 attttggatt ttagtttgga taatttgcct tcctgctctt cagggtgttc ggtaaatagt
     1981 tctttactta caatgaatcc aagaatggaa tcgcccagaa actccatccg ttcaaaagga
     2041 gaaggagttt tatgttcact ataatgacga tggagatagg atttatgtgt taaagcagca
     2101 tgcaataaag tggggtcatg aaaagtataa tcaattttct tttgcaactg cgtaagtgat
     2161 ttttcccatt tgggatactt ttcacctatc tttttagtgt tgaaatactc aacaatccgg
     2221 gagatgattg attttgtcct tttctccatc gatgtatata tcccggcttg atttattgat
     2281 agcgtttaat gattatagca gaattatggc ctccaaaacc aagggaattg gaaagggtta
     2341 tatctacttg gtgatgaata gctacattag gagtgtaatc caaatcacat tctggatcag
     2401 gatgagttaa atttattgta ggatgaatga tcccattttc aattgtctta acacaaacga
     2461 tagcttcaat ccctgcagca gctcctaaca tatgaccaac catactttta gtggaattta
     2521 tctttacttt gtaagcataa tctccaaaaa cagttttgat ggaatgagtt tcgcctttat
     2581 catttaaagg tgtagaagtg ccatgtgcat ttatataatc aatatctgta ggttttatac
     2641 cagcatcatt tattgccatt aacatagccc gtgcacttcc ttctccattt tctgtaggag
     2701 cagttatgtg aaaagcatct tcagaagcac cgtaacctat taattcagca taaattttag
     2761 cacctctggc tatagcatgt tccagttctt ccaaaacaag aattgctgct ccctcactca
     2821 ttataaaacc atcccgttct gcatcaaaag gacgcgatgc tttttcagga tcatcattac
     2881 gagtagaaag agctctcatt gcagaaaaac cagctatcga taaaggagtt acagctgctt
     2941 catttccacc gctaagaatt atatctgcat ccccatattg aatagctcta taagctgttc
     3001 ctatagcatg attggcactg gcacaagcac tcatcacatt gaaattgata cctttgaagt
     3061 tattctctat actaatatgg gcagcagcaa tgttaccaat cattttgggt ataaaaaaag
     3121 gactcactcg acggggaccc attgtataat tcttgatgca ttcttcttca aaagtaagaa
     3181 taccaccaat tcctgcaccg gttattactc ctacccgatt ggggtcataa gaatttggag
     3241 taagacctgc atcagcaatt gcttcgcggg ctgcaatcat agcaaattga gcatataaat
     3301 ccagcttttt tatctcttta aattcaaaat gatcttcagg attgtaattc ttcacttcgg
     3361 cagcaatatg aaccggcaaa ttatctatat caaatcttga tattaatcct acgccagaaa
     3421 ctccgttaat aagggattgc caggtttggg aaacattgtg tcctacagga gtaatggcac
     3481 ctaaaccagt tataacaact cttcttttat tcataaaaac ctcattaagt ttaacaaatt
     3541 atttgtcttt aataccactt ttctcttccc aaccttgtat ttaaggcaaa aaaatcaaga
     3601 gaaaacatcc tctgtgcgtg gatacaatcc tgatatctat tatgttatca ggattgatcc
     3661 acattagggg gatgggtatt tatcccagtt tttctttcag atagtcaatc gcttgaccga
     3721 ctgttttcag tttatcctga tcttcttcag ggatagtgag atcaaattct tcttcgaaag
     3781 ccataacgag ctcaactgta tcgagggaat cagctcgtaa gtcatcgatg aaatttgcgg
     3841 aaggaacaac ttgggattcc gggactccca gttttttcgc tactatctgt tttacttttt
     3901 cttcaatatc cattattcct cctatgttat tgaagtgatt atttatctat tggtttccca
     3961 tttaatgcat tgttaaaccg ccatctacgg ctatagtttg tcccgttaaa taacttccag
     4021 cgtcggaggc tagaaagaga catacattag ctatatccat aggggttccc attttttgca
     4081 agggtatggc ttttgcatat tctgctctgg tttcagaact taaagatgct gtcatctcag
     4141 tttctataaa acctggagca actgcattaa cacgaatatt acggctacca aattcttttg
     4201 cgcaactttt ggtgaaagca ataagaccac ctttggaagc ggcataattg gcctgtccac
     4261 tatttcccat aattccgata acactggcga tattaataat acagccatta cgggaacgca
     4321 tcatatattt aaaagccttt tgagtgcaaa gaaaactacc ttttaggtta atatccataa
     4381 ccaattgcca gtcctcttct ttcattctga tcaataaatt atcgcgagtg attccagcat
     4441 tattgatcag gcaatctata gttccatatt tatcaactac atcagcaaaa atagtttcaa
     4501 tacttttggc gtctgtaaca tttcctacat agccataggc ctggaatcca ttttcttgta
     4561 actctgctat tgctttctct actgcttctg aagcaacatc tataattata acttttgcat
     4621 ttgcattagc aaaagtttta gctatggcaa aaccgatccc gcgagcggca ccagtaatta
     4681 cgataacctt atctgtaaaa tcaagtttca ttatctcctc atctgattgg tttaattata
     4741 taaccagctt aatttgtaac tgacacaact gcatctatat cttcatattt gtcaatagaa
     4801 aaaacttctg catctctatt gatatttcta atcattccgc ttaaaacctt ttgcggacca
     4861 aattcgagga aacggttaac tccctgttca atcataaatt gaacactttg ca
//
LOCUS       DAORHB010000105         4904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_18070_length_4904_cov_4.476387, whole genome shotgun sequence.
ACCESSION   DAORHB010000105 DAORHB010000000
VERSION     DAORHB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18070_length_4904_cov_4.476387"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="PKM80_03990"
     CDS             <1..1493
                     /locus_tag="PKM80_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNV93102.1"
                     /translation="RAALGAGAIVMDLIASNDRRMNYEKIERIRQLRPDMILMAGGED
                     GGTVKHVVEMAELVASADPKPRLGSGYKLPVIYAGNKDAREEIIKTLGEKVDLIITDN
                     IRPKLEIENLLPAREKIHDLFMEHVMKQAPGYNKLMEWTIGPEHEQVPIMPTPAAVGN
                     IMQAIAKEEQIEVLGVDIGGATTDIFSVFTKDFVFNRTVSANLGMSYSISNVLASAGL
                     DNIMRWVPFDINENELRNMIKNKMIRPTTVPSLLEELVLEQAIAKEALRLAFEQHKSF
                     ASSLKGMQKQRDISEAFSQSLSGETIVNMLTLDLLVGSGGVLSHAPRRNQTVMMLIDA
                     FLPEGITRLSVDSIFMMPHLGVLSEISTKAATEVFNKDCMVYLGTCVAPVGKGKIGKP
                     ALYAKLELPDGSVFEEDIPFGEIRLIPCEVGQKAKATLKTHKGLILDQDKKKEITVDL
                     HGGIVGIVIDTRGRQPFVLPTEKSERIAALKKWMKELKAYPEHILV"
     gene            1503..2633
                     /locus_tag="PKM80_03995"
     CDS             1503..2633
                     /locus_tag="PKM80_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93103.1"
                     /translation="MGQAYSAGLTVTDNIILRKERILPLKGQVVVKKGDKVKAEDVVA
                     ETLLPGKVIPFNLANKLGVTPQQLENYIKIQPGQKITKNTVLAANKGLFGLGIMKNEV
                     LSPIDGEVENISSVTGQILLREPKIPVQVKAFIDGLVTDVIEDEGVIIENKSAYIQGI
                     FGLGGETYGELKILAESPDDELDVAKIDASCAGKIIVAGAFAAYHSIDIARKNGVKAI
                     ITGGLDDQDIKKLLGYDIGVAITGHENIGITIVCTEGFGKINMANKTFELLKRFEGHK
                     TSIHGRTQIRAGVIRPEIIIPLEFDEKELVAKTSSMPVLEIGTVIRIIRQPHFGQIAK
                     VTALPEELTKVESETLVRVLEAEFEDGTRTILPRANVEVIES"
     gene            3305..4825
                     /gene="proS"
                     /locus_tag="PKM80_04000"
     CDS             3305..4825
                     /gene="proS"
                     /locus_tag="PKM80_04000"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNV93104.1"
                     /translation="MSKNNKTAITPTREENYAEWYQQVIKAADMAENSEVRGCMVIKP
                     WGYAIWENIQHILDRMFRETGHRNAYFPIFIPKSFFEKEAEHVEGFAKECAIVTHSKL
                     EADEKGGLKLAGELTEPYIVRPTSETIIGASFAKWVNSYRDLPLLINQWANIVRWEMR
                     TRLFLRTTEFLWQEGHTVHETEIEAREETLKMLNVYATFARDYLALPVIQGEKTESEK
                     FPGAVTTYCIEAMMQDRKALQSGTSHFLGKNFAKASEIKFQNRNGEMEYAWTTSWGVS
                     TRLIGALIMAHSDDNGLVLPPKIAPSHIAIIPIYKNDEEKQIVMDFAIKVQNIFKGLY
                     LDDIQIFTEMDTRDMTSSERNWYWIKKGIPLRLEIGPRDIVNDSVMLARRDKEPKDKH
                     SIKIDNVVDYVLKTLSEMQEGYYQRALNFRDKWTVDIDDKEEFYRFFNSKNEEQEISG
                     GFANSHWCGNQTCEEKIKDDLKVTIRCIPFEAKEEEGKCIYCGEKSNRRVIFAKAY"
BASE COUNT         1637 a          814 c         1038 g         1415 t
ORIGIN      
        1 aaagagcagc tttgggagca ggggcaattg ttatggacct cattgccagc aacgatagaa
       61 gaatgaacta tgaaaaaatt gaacgcatcc gtcaacttag gccagatatg attttaatgg
      121 ctggaggtga agatggaggc actgtaaaac atgttgtaga aatggcagaa cttgtagcca
      181 gtgcagatcc taaacctcgt ttgggttccg gttataaact acctgttatt tatgctggaa
      241 ataaagatgc ccgcgaagaa attattaaaa ctctgggcga gaaagtagac ctgataatta
      301 ccgataatat tcgtcccaaa ctggaaattg aaaacctcct gccagcaagg gaaaagattc
      361 atgacctatt tatggaacat gttatgaaac aagcacctgg ctataataaa ctgatggaat
      421 ggactatagg ccctgaacat gaacaagttc ctattatgcc tactccagcc gcagttggaa
      481 atattatgca agcaatagct aaagaagaac aaattgaagt tttaggagtt gatattggag
      541 gagctacaac cgatatcttt agcgttttca ctaaagattt tgttttcaat cgtaccgtta
      601 gtgctaattt gggaatgagt tatagcattt ccaatgttct ggcgtcagca ggattggata
      661 atattatgcg ctgggttcct tttgatatta acgaaaatga attgcgcaat atgattaaaa
      721 ataaaatgat ccgtccaaca actgttcctt ctttgttgga agaactagtt ttggaacagg
      781 caatagctaa agaagcatta cgtttggctt ttgaacagca taaatccttt gccagttcac
      841 tgaaaggtat gcagaaacag cgtgatattt ctgaagcatt cagccaatcc ctttccggag
      901 aaactatcgt taatatgctt actttagacc ttttagtagg tagtggagga gttttatctc
      961 atgctccacg cagaaatcag accgtgatga tgttaattga tgcttttcta ccagaaggaa
     1021 ttactcgtct ttctgtagac agcattttta tgatgcctca tctgggtgtg ctttccgaga
     1081 ttagtaccaa agcagctact gaggttttta acaaagattg tatggtttat ttaggaactt
     1141 gtgttgcccc tgtagggaaa gggaaaattg gtaaaccagc actttatgcc aaattagaac
     1201 ttcctgatgg ctcggttttt gaagaagata taccctttgg tgaaatccgt ttaataccct
     1261 gtgaagttgg tcaaaaggca aaagcaactc ttaaaaccca caagggcttg attttagacc
     1321 aagataagaa aaaagaaatt accgttgatc ttcacggtgg aatagttgga atcgtgatag
     1381 atacacgagg acgtcagcca tttgttcttc ccaccgaaaa atcagaacgc attgctgctc
     1441 tgaaaaagtg gatgaaagaa ttgaaagcat accctgaaca tatcttagta taggaggaaa
     1501 aaatgggaca agcatattcc gctggattaa ccgttaccga taatataatc ttacgaaaag
     1561 aacgcattct acccctcaaa gggcaggtag ttgttaaaaa aggtgataaa gttaaagctg
     1621 aagatgtagt tgctgaaact ttattacctg gaaaagtgat acctttcaat ctggctaata
     1681 aattgggtgt aactccccaa caattagaaa attacataaa aatccaaccc gggcaaaaga
     1741 ttacgaaaaa cacagtttta gctgccaata aaggtctttt tgggctggga ataatgaaaa
     1801 atgaagttct ttctccgatc gatggagaag tagaaaatat ctcttctgtt actggacaaa
     1861 ttttactgcg cgaaccaaaa atccctgttc aagtgaaggc atttattgat ggattagtta
     1921 ctgatgttat tgaagatgaa ggtgttatca tagaaaataa aagtgcctat atacaaggca
     1981 tttttggttt ggggggcgag acctatggcg aattaaaaat acttgcagaa tctccggatg
     2041 atgaattgga tgtagctaaa attgatgctt cttgtgcagg aaaaattatt gttgcaggtg
     2101 cctttgctgc ttatcattca atagatatag ctcgtaaaaa tggcgtaaaa gcaattatta
     2161 ccggtggact tgatgaccag gatattaaaa aattactcgg ttacgatatc ggggtagcca
     2221 ttaccggaca cgaaaatatc ggtataacta ttgtttgcac agaaggtttc ggcaaaatca
     2281 atatggctaa taagaccttt gaacttctga aaagatttga gggacataaa acctctatac
     2341 atggcagaac tcagatccgc gctggagtta tcagaccaga gatcattatt cctctcgagt
     2401 ttgatgaaaa agaactggtc gctaaaacaa gctctatgcc tgttttagaa attggaacgg
     2461 tgattagaat tatccgtcaa ccgcattttg gtcagattgc caaagttaca gcacttccag
     2521 aagaacttac caaagtggaa tcggaaaccc tggttagagt actggaagca gaatttgaag
     2581 atggaacaag gacaatacta cctcgtgcta atgtggaagt gatagaatca taggagttta
     2641 aacaaactcg taaacaacat aactaagatc caagttttgc gtaaccgatg tgcaatatta
     2701 aagggatgtc ctctgctcgg taaacggaac ttgattatat aaaatgtagc ctttcaggaa
     2761 tatttgacat ttccccaaat aacttgaggg ggaatgtcat ttttccttat caacccaact
     2821 ttttttaaga gtctctcttt tctaggatac tgcctagtat gccatttctg gaaatcgggt
     2881 tgtttcttgc ttggttcttg tggcgcaatt gccgtggcac taatggggaa attgccgatc
     2941 agttccactg cgtctccagc gctgttatta aggaagaaca tagatatatg cagcgaaaat
     3001 tgtaacatta cgaaccgcaa aatctgcact cgcaaaacag ctaagtatct ataaataata
     3061 gttgttcagc ttttaataat cacctaatta ttcctgtaaa ataagcacat atttgtttca
     3121 taaccagata aatatttgca aagcagggtt tataaagcac aaatgaagtt cttttgcatt
     3181 gacaatattg gcacaataaa ggaattggct ttttttaagt gaaataatat aatgggtatt
     3241 atgtctgctg agaaagaaaa aacagatacc ctaagtgttc agtatgtaat tatggagaaa
     3301 agtaatgagc aagaataaca aaactgcaat tacccctact cgggaagaaa attatgccga
     3361 atggtatcaa caagtaataa aagcagccga tatggctgaa aatagtgaag ttcgcggttg
     3421 tatggttata aaaccttggg gctatgctat ttgggaaaat attcaacata ttctggatag
     3481 aatgtttcgc gaaacggggc atagaaatgc ctattttcct atttttattc ccaaaagctt
     3541 ttttgaaaaa gaagctgaac atgttgaagg ttttgctaaa gaatgcgcta tagtaacaca
     3601 tagtaaattg gaagcagatg aaaaaggcgg attaaaactt gccggagaac ttactgaacc
     3661 ttatattgta cggcctacaa gcgaaacaat tatcggtgca tcctttgcca aatgggttaa
     3721 ttcctatagg gatttgcctt tgttaataaa tcaatgggct aatattgtgc gctgggaaat
     3781 gcgtactcgt ctatttttaa gaaccactga atttctttgg caggaagggc acactgtgca
     3841 tgaaacagaa attgaagctc gagaagaaac cttaaaaatg ctgaatgttt atgctacatt
     3901 tgcgcgtgat tatcttgctt tgcctgtcat tcaaggtgaa aagactgaaa gtgaaaaatt
     3961 tcccggtgcc gttacaactt attgtattga ggcaatgatg caggatagaa aagcattgca
     4021 atcgggcact tctcactttt tgggtaaaaa ctttgccaaa gcctccgaaa ttaaatttca
     4081 aaatcggaac ggagaaatgg aatatgcctg gactacttct tggggtgttt ccacgcggtt
     4141 aattggtgcc ttaattatgg cgcatagtga tgataatgga ttagttttac ctcctaaaat
     4201 tgctccttcg catattgcga ttatccctat ttacaaaaat gatgaggaaa aacagatagt
     4261 gatggatttt gccataaaag tgcagaatat ttttaaagga ttgtatttgg atgatattca
     4321 aatatttact gaaatggata caagggatat gaccagcagc gaaagaaatt ggtattggat
     4381 aaaaaaaggt attcctctac gcctcgaaat aggccctcgt gatattgtta acgattctgt
     4441 aatgcttgcc agaagagata aagaacctaa agataaacac tcaattaaaa ttgataatgt
     4501 ggtagattat gttctaaaaa ccttgtccga aatgcaagag ggctattatc agcgggcttt
     4561 gaattttaga gataagtgga cagtagacat tgatgataag gaagaattct atcggttctt
     4621 taattccaaa aatgaagaac aggaaataag cggtggtttt gccaattccc actggtgtgg
     4681 aaatcaaacc tgcgaagaaa agataaaaga tgacctgaaa gttacgatcc gctgcattcc
     4741 ttttgaggca aaagaagaag aaggcaaatg tatctattgt ggtgaaaaat ccaatcgtag
     4801 agttattttt gctaaagcgt attaaaatga atgcagccat catcaatatt ggcaatgaaa
     4861 ttttgatggg taaaaccatc aataccaatc ttgcctattt gggt
//
LOCUS       DAORHB010000106         4876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_18216_length_4876_cov_4.957063, whole genome shotgun sequence.
ACCESSION   DAORHB010000106 DAORHB010000000
VERSION     DAORHB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18216_length_4876_cov_4.957063"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1451..1591
                     /locus_tag="PKM80_04005"
     CDS             1451..1591
                     /locus_tag="PKM80_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93105.1"
                     /translation="MLESRETINYITSSYGHASKRSDLHKDEYYESILKQAVSENENA
                     KS"
     gene            complement(1765..2235)
                     /gene="rpsG"
                     /locus_tag="PKM80_04010"
     CDS             complement(1765..2235)
                     /gene="rpsG"
                     /locus_tag="PKM80_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNV93106.1"
                     /translation="MPRKRRAVVREVLPDPKYNDITLTKFMNCLMNQGKKSIAEKIVY
                     GAFDIIAEKTKEEPLVVFKSALENVRPLVKVVSRRVGGSSYQIPLEVNEKNGRALAFR
                     WIISNARARSEKTMTEKLAAELIAAYKKEGASIKKREDTHKMAEANKAFAHLRW"
     gene            complement(2252..2638)
                     /gene="rpsL"
                     /locus_tag="PKM80_04015"
     CDS             complement(2252..2638)
                     /gene="rpsL"
                     /locus_tag="PKM80_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNV93107.1"
                     /translation="MPTINQLVRKGRADVEKKRKNRALMECPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLVNGFEITAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DSAGVEKRERSRSKYGTKRPKAKSGK"
     gene            complement(2677..>4876)
                     /locus_tag="PKM80_04020"
     CDS             complement(2677..>4876)
                     /locus_tag="PKM80_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNV93108.1"
                     /translation="GLTVLEYFVSTHGARKGLADTALKTADAGYLTRRLVDVAQNAII
                     TIEDCDTTRGVNMTALKEGNEIVQTLAERIQGRTAVDDVVSPITGRVLVSAGEEISNK
                     MARTIQNHGIISVHVRSVLTCEAERGICAKCYGRNLATQKPAKLGDPVGIIAAQSIGE
                     PGTQLTLRTFHIGGAASTATELAEVVASHDGIVKFDRMNTVTNPENQLISVSHLGRIL
                     IVDEQDDSKVLEEFKVEYAATVHVQDGQKIAKDTKLLSWDQFNNPLISTAKGVLHYEN
                     FIKDITYKEEYNDITLARELTIIESKDRRKQPQFRIVGEDGTVRLIPLPTGLIVRVED
                     GSFVHTGDILGQTSRMTIKQRDITGGLPRVQELFEARVPKDKAKISDIDGIVTIGGLK
                     KTGRDIFVTPPNGLFAPFDGKVSIYSDEDKNQYIAVLSDKAVLSENDGKVTILEENRK
                     KIIQVAKRGQKPVQYSVPKGMQILVKEGESVKTGTPLCGKLYAVPPEQESIVSTGDSV
                     SIHQPLAGRKYIIPSGKRIIVHQGDYVESGDALSDGPLDPHDMLVKGVIEAQILILNE
                     IQEIYRKQGVKIDDKHVSVIIRQMFKKVRITDSGSTSFLQGDIVDKILVEKENREAIS
                     FGKTPAQFEQLLLGITKTSLLTDSWLSAASFQETTKVLTKAAIEGRVDHLEGLKESII
                     IGHRIPVGTGAKLYNNMIKQAVNEGKTVAQIIDEYAHHAEEEESEDYLNF"
BASE COUNT         1457 a          958 c          925 g         1536 t
ORIGIN      
        1 attacggaac cgatcccgat attgaagtgg atattatgcc tcaggattat gttcagggaa
       61 aagacccgca actggatgtc gccatcaaaa cctgcctggc agaaataaag aaaaatcctc
      121 cgctgaaacc ggattttggg aaaatcccgc gtaagacatt gccctaaaat accgaagata
      181 tttcacgcag atttaagctg agaaacaagg atttaaggat tagaggatta aaaggattta
      241 ttgattagcc accgaaaaca ccgaaaacac cgaaagtaca actaactatg caaaacggct
      301 gtagctcttt actgcagccg ttttttctat tgccaaaaat ggaattatat aacaggcatt
      361 gttttgttat tatgctaaag gtaatgaata agagaatata tttcccgcag atttgagttt
      421 ggaaacaagg attttaggat ttgttcacga agatttaagt ttagaaacaa ggatttcagg
      481 atttgttcac gaagatttaa gtttagaaac aaggatttta ggattatagg atttagagga
      541 ttatatatta tatatgatga ctgtctaagt gattatttaa gccccaaagg gcgacacaat
      601 catagcgacg gtggcgtaag tccttcggaa aaaagataat aggtaaggga tgatcaggat
      661 tttagggatt ttttggattt aagtttagaa acaaggattt tagggatttg ttcacgaaga
      721 tttaagttta gaaacaagga tttcaggatt taaggattaa gaggattccg aataaaagct
      781 ccgttaggaa cgatacatac taacgacggg ttttaacccg gcgcaatgta gaacaaataa
      841 attagtccca tcgggacgac agatattagc gacgggtttt aacccggcgc aaatagtaac
      901 agataatatt attcccatcg ggacgacaga tttgagcgac gggttttaac ccgtcgaaaa
      961 tacagaaagg ttgacttctt cgtcaacaac aacaaacaaa ctatcaggca attataaaag
     1021 aaagcggaaa ctgcataact cggttgtcag gggaacgaca ccctgtcgtt cttcctgaaa
     1081 aatattctct cacagattgc atagataaca cagataaagg tctcacgcag atttaatagg
     1141 taggggatga acaagatttg tgggattttg gggattttag attgcaatta aataagctgg
     1201 ttttctgaat gttatatatg gaaatagagt tttaaaatgc accagttttg gcaaaggaaa
     1261 atgaaacaaa gataaaattg acagaaattg tgtattgaga taacttccat acagcttaag
     1321 aagttgaggt tgggattaac ctccaggcct ctcgacttcc agaccataaa ttcatacttg
     1381 cttcaacatc taataatata gtaacagttc tttaacattg ccgttacgaa atatgagaag
     1441 tgttacttct atgttagaga gtcgtgagac aataaactat ataacaagca gttatgggca
     1501 cgcaagcaaa agaagcgatc tgcacaaaga tgaatattat gaaagcattc taaaacaagc
     1561 cgtaagtgaa aatgaaaatg cgaaaagtta gtgaatttgt agcgcaagaa caatctctac
     1621 tttgccataa atattggata gttctgaaaa aattagccta cctacatcag ttgaattagc
     1681 agttagtaac ctataaaaga aaaatgcctt tactctcaaa cacaggtaaa ggcattttac
     1741 aattcaggaa ttatgaaagt taaattacca cctgaggtgg gcaaatgctt tattggcctc
     1801 agccatttta tgggtatctt cccttttttt aatggaagca ccttcttttt tataagcggc
     1861 aataagttcg gcggcaagtt tttccgtcat tgttttttcg cttctggctc gtgcattgga
     1921 aattatccaa cgaaaagcta aagctctgcc gtttttttca ttaacctcaa gaggaatttg
     1981 ataactggaa ccaccaacgc gtctagaaac gactttaact aaggggcgca cattttccaa
     2041 agcagattta aaaacgacaa ggggttcttc tttagttttt tctgcgataa tatcgaaagc
     2101 accatatacg attttttctg cgatgctttt cttaccttgg ttcatcagac aattcataaa
     2161 tttggtaagg gtgatatcgt tgtattttgg atccggaagg acttcccgga caacagctct
     2221 acgttttctg ggcataaatt atatcctccc tttattttcc cgatttagct ttgggacgtt
     2281 tagttccata tttggaacga gatctttcgc gtttttctac tcctgcagaa tcaagagctc
     2341 cgcgaacaat atgataacga acaccaggaa ggtcttttac acgacctccg cgcactaaaa
     2401 caatagagtg ctcttgtagg ttgtgacctt ctccaggaat atatgctgta atttcaaatc
     2461 cgtttacaag acggacacgg gcaacttttc taagagccga attaggtttt ttgggcgtag
     2521 ttgtatatac tcttgtgcat actccgcgcc tttgcggaca ttccataagc gccctgttct
     2581 ttcttttctt ttcgacatcg gctctacctt tacgaaccaa ttgattaatg gttggcactg
     2641 attttctcct tttatcttat tgagttatag tgtattttag aaattaaggt aatcttcact
     2701 ttcttcctct tccgcatgat gagcatattc atcgattatt tgggcaacgg ttttaccttc
     2761 attcactgct tgtttaatca tattattata gagttttgcc cctgttccta caggaatacg
     2821 gtgaccaata atgatgcttt ctttcagacc ttccagatga tcaactcttc cttcaatagc
     2881 agcttttgtg agtacttttg tggtctcctg gaacgaagcg gctgaaagcc agctatcggt
     2941 taacaaagaa gttttagtta ttcccagaag caattgttca aattgagcag gtgttttacc
     3001 gaaagatatt gcttccctat tttctttttc caccagaatc ttatctacaa tatcaccttg
     3061 tagaaaactg gtgctaccac tatcggtaat tctaaccttt ttgaacatct gacgaatgat
     3121 tacgctaaca tgtttatcgt caattttcac gccctgttta cggtatattt cttgaatttc
     3181 attcagaata agaatttgag cttcgataac acctttcact aacatatcgt gaggatctaa
     3241 tggaccatct gataaagcgt ctccactttc cacatagtca ccctggtgaa cgataattcg
     3301 ttttccagaa ggtatgatat actttctacc agcaagcggc tggtgaatag aaacgctatc
     3361 accagttgaa acgatacttt cctgttcagg tggaactgcg tatagtttac cgcaaagagg
     3421 agttcccgtt ttaacggatt caccttcctt aactaaaatt tgcatccctt tgggtacaga
     3481 atattgaacc ggtttctgac cgcgtttagc tacctgaatt atcttcttgc gattttcttc
     3541 taagatagtt actttaccat cgttttcact caatactgct ttatcagaaa gcactgcaat
     3601 atactgattt ttatcttcat cggaataaat agatactttt ccatcaaatg gagcaaaaag
     3661 tccattagga ggagtaacga aaatatctct tccggttttt ttcaagccac caattgttac
     3721 aattccatca atatcggaga tcttggcttt atctttggga acccttgctt caaaaagttc
     3781 ctgcactctt ggtaacccac ctgttatatc acgttgtttt atagtcattc tggaggtctg
     3841 gcctaaaata tcgccggtat gcacaaagct tccatcttcc acgcgaacaa tcaatcctgt
     3901 tggtaaagga attaaccgta cagttccatc ttcgccaaca attctgaact gcggttgttt
     3961 tctacggtct ttggattcaa taatagtaag ctctcgagca agagttatat cgttatattc
     4021 ttctttataa gttatatcct taataaagtt ttcatagtgc aacacaccct ttgctgtaga
     4081 aataaggggg ttattgaact ggtcccaaga aaggagcttc gtatctttgg caattttttg
     4141 accatcctga acatgaacag ttgccgcata ttctacttta aattcttcta acactttact
     4201 atcgtcctgt tcgtccacaa tcaaaattcg acctaaatgg ctaacggaga taagctgatt
     4261 ttcgggattg gtaactgtat tcatcctatc gaattttaca ataccatcat ggctggcaac
     4321 aacttctgcc aattctgtag ctgtagaagc agctcctcca atgtggaagg ttctaagtgt
     4381 taattgtgtt ccaggttcac caatactttg agcggcaata atacctactg gatcacctaa
     4441 ttttgcaggt ttctgagtgg caagattgcg tccgtaacat tttgcacata ttcctctttc
     4501 agcttcacaa gtaagaacac tgcgaacatg tacagaaatt ataccatggt tttggatagt
     4561 tctggccatc ttgttgctta tctcttcacc tgcagaaact aaaactcttc ctgtgatggg
     4621 acttacaaca tcatccacag cagttcttcc ctgaattctt tcggccaggg tttgtacaat
     4681 ttcgttgcct tctttgaggg ctgtcatatt cacccctctg gtagtatcac aatcttcaat
     4741 tgtgataata gcattttggg caacatctac taacctacgg gttaaatatc ctgcgtcagc
     4801 tgtttttaac gctgtgtctg ccaatccctt acgcgctccg tgagtggata cgaaatattc
     4861 aaggacagta agccct
//
LOCUS       DAORHB010000107         4864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_18279_length_4864_cov_4.330630, whole genome shotgun sequence.
ACCESSION   DAORHB010000107 DAORHB010000000
VERSION     DAORHB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18279_length_4864_cov_4.330630"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /gene="ispF"
                     /locus_tag="PKM80_04025"
     CDS             complement(<1..430)
                     /gene="ispF"
                     /locus_tag="PKM80_04025"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNV93109.1"
                     /translation="MIRSGFGYDVHRLVKDRELILGGVKIPHSKGLLGYSDADVLLHS
                     IIDALLGALALGDIGTHFPDNDLAYKDIDSRLLLRKTYILISGKGWKINNLDATICCQ
                     EPKLKPFIYDMRKNIACDLNCDLEQISVKATTEEGLGISGK"
     gene            complement(434..3178)
                     /locus_tag="PKM80_04030"
     CDS             complement(434..3178)
                     /locus_tag="PKM80_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNV93110.1"
                     /translation="MWFFLHSLLKRLLSIIRKWELRIPIPVFGFGCGVLGLALPKVSL
                     NMGVYASKLLKSLEYRGYDSTGAAFQGDTTEITLLKDVGAPSTLVKTLGIEKQSGKIF
                     CGQVRWATFGFVDKINAQPHEVNCKRHIYGAHNGNITNTGELKSFLLNQGHFVQSDND
                     GEMLVHIIEHYFDIEMDKTGNPKAPEERKNCMRQAIIQAANKLVGSYAAVIVDPDTET
                     SWAIKAGSSLYFGIGTIEGMPFSLASSDLTAVLRFTKQLVNLREGEFIEYTAENYQVY
                     AQKDLKFKRLNQPNEIWKTGDQIPTKPVYSKLRAEDVELLPEYEYFMEQEIYAQSETT
                     GKLIKLFQGGSNTGKRMLALMDKEGVRDFLQEQVKQFLDAHLPEERQKVFYDLLNSET
                     FSNFFNQVRSTYKEFFDVAVKEDFDKKYFFSSEKNLFLEMANNGYDLKKISLAKTMDS
                     IAEEMNVKDFNDSIEDFLLLAKNTILNSRNAYSIACGTSFHATKIAALFFNTIAGMEI
                     IPILPGDFRGEYSNCIKDNDLIIGVSQSGETKDLIDIFNDIDAKNLNVRKVVLVNNMN
                     STLGQEKSDVAIPILCGPEIAVPATKSFMNQITLFYYLAIRTAQMKLNEIDTHFSTEQ
                     REKHQQELDGYFHSLSKIPSLIKETLENVSGELDIMAGKLYMEPSIHILATKISGVAM
                     EGALKIRETVLTHAEGREASEFKHGPNTILGKNTVFGVKHLRSLLHYFSENIDAVESL
                     CEQEAIPHKEIKDIYKALAEYIFTHNQPFNLSLRGTKVFNKIVKDKDFFEPLYRNYPL
                     IYVTGPNERDVNLTISQINTHKIRGANTFVIAEENEQLMKNVASRPSENSYYAWSYIM
                     LPKTGSTMMTCFSASAVLQLLALKLSIRKMKRLDKLEVRDHGVHPDVPKNVSKSITVD
                     "
     gene            complement(3190..3894)
                     /gene="ispD"
                     /locus_tag="PKM80_04035"
     CDS             complement(3190..3894)
                     /gene="ispD"
                     /locus_tag="PKM80_04035"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNV93111.1"
                     /translation="MDFLKNTTAIITAAGSGKRLPGSSKKQFRELVGIPILIRSMEPF
                     MASELIDNIIVTVPEEDITKTEALIEQWFEGFTKPYKIIPGGAERQDSVFSALKACPE
                     GTEYVAIHDGVRPFVTLELIELLFAAVTVDKAVVPASKLKHTVMQIEDNYAVRTINRN
                     QLVNVFTPQVFEYALIMEAYIAAYRNNFYSTDDASLIQNLGERIRVLWSDDFNIKITD
                     EADLFFAKQLIEKNKI"
     gene            complement(3882..4859)
                     /locus_tag="PKM80_04040"
     CDS             complement(3882..4859)
                     /locus_tag="PKM80_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV93112.1"
                     /translation="MYNLEPHFKMIKYKHLFGPVMSRRLGISLGIDLVPYKYCPLNCV
                     YCEVQKTTHQVTKRESYFEVREILDELDNFMATAPHLDYITFSGAGEPTLNSSIGEIV
                     NYIKAKYPSYKLALLTNGVLFNDKEVRQEILPCDIVLPSLDSATQEGFEKINRPCPEL
                     KVQDLIEGLVQFRKEYKGTIWLEVFLIKGINDTEEEIEALAKAINRIKPNLVQLNCLD
                     RPGAEDWVNPIPLHNLKQIKAQFKEKTSVPVEIIAKVKYKTTESQLDTELLEMLHSTL
                     VRRPSTAEDLSAMLDIHINEISKVLRQLCLEKKITVKRESRGVFYIWTS"
BASE COUNT         1495 a          973 c          771 g         1625 t
ORIGIN      
        1 ccttgccact gatgccaagt ccttcttcag tagtagcttt cacagatatt tgttccagat
       61 cacaatttaa atcacaggcg atattttttc gcatatcata tataaacggt tttaatttgg
      121 gttcctgaca gcaaatagtg gcatccagat tattgatttt ccagcccttt cctgatatta
      181 agatgtatgt tttacgaagc agtaaacggg aatctatatc tttgtaagct aaatcgttat
      241 caggaaaatg tgttccaata tcacccaaag ccaaggcacc taaaagagca tcaattatgc
      301 tatgcagtaa aacatcggca tctgaatagc ccaaaagccc tttggaatga ggaattttca
      361 cccctcccaa aataagttcc ctatctttca caaggcgatg aacatcatag ccaaaaccgc
      421 tacgaatcat aaatcaatcc acagttatac tttttgaaac atttttaggt acatcaggat
      481 gaacaccatg gtcacgcact tccagtttat ccaatctttt cattttacga atgctaagtt
      541 ttagagctaa aagttgtaaa actgcagaag cggaaaaaca ggtcatcatc gtggaacctg
      601 tttttggtaa cataatataa gaccaggcat aatagctatt ttcgctggga cgagaagcaa
      661 catttttcat taactgttca ttttcttccg ctatcacaaa tgtattagct ccccttattt
      721 tatgggtatt tatttgcgat attgtaagat ttacatcgcg ttcattgggt ccggttacat
      781 aaattaaagg ataattccga taaaggggtt caaagaaatc tttatctttc acaatcttat
      841 tgaaaacttt agtgccgcgc agagatagat tgaagggttg gttatgcgtg aatatatact
      901 ctgccaatgc cttatagata tcctttatct ccttatgagg aatggcttct tgttcacata
      961 gagattctac tgcatctata ttttcactaa aataatgcag caacgaacgt aaatgcttaa
     1021 ccccaaaaac ggtattctta cctaagatag tattaggacc gtgcttaaat tctgatgctt
     1081 ctcttccttc tgcatgagtt aaaactgttt ctcttatttt taaagcacct tccatagcca
     1141 caccacttat ttttgtagct aaaatgtgaa tagaaggttc catatataat ttcccggcca
     1201 taatatctaa ttcgccgctt acattttcca aggtttcttt tattagcgaa ggaattttag
     1261 atagagaatg aaaatatcca tccagttcct gttgatgctt ttccctttgt tctgtgctaa
     1321 aatgtgtatc aatttcattt agtttcattt gagctgtgcg aatagcaaga taatagaaaa
     1381 gggtgatttg attcataaag ctttttgtgg ccggaacagc aatttctggt ccacacaaaa
     1441 taggtatggc cacatcactt ttttcctgac ccaaagtaga attcatatta ttcactaaaa
     1501 ccactttccg cacattcaaa ttcttggcgt ctatatcatt aaagatatca ataagatctt
     1561 ttgtttcacc cgattgagaa acaccaataa taagatcgtt atcttttatg cagttagaat
     1621 attctcctct gaaatcacct ggtaaaatgg ggataatttc cattcctgca atggtattga
     1681 aaaacaaagc agcaatttta gtagcatgaa aacttgttcc acaggcaata gaataggcat
     1741 tgcgactatt taaaatagtg tttttggcaa gtagcaaaaa gtcctctatg ctatcattaa
     1801 aatctttcac attcatctct tcagcaatgg aatccattgt ttttgccaga gagatttttt
     1861 taaggtcata tccgttgttt gccatttcca gaaatagatt tttctcgctg gagaagaagt
     1921 actttttatc aaaatcctct tttacggcaa catcgaaaaa ttccttataa gtgctgcgaa
     1981 cctgattaaa gaaattgcta aaagtctcgg aattaagtaa atcataaaat actttttgcc
     2041 gttcttcggg taaatgtgca tctaaaaatt gtttaacctg ctcctgtaaa aaatctcgca
     2101 caccttcctt atccattaaa gctaacattc tttttcctgt attggagcca ccttgaaaaa
     2161 gcttgatcag cttgccagta gtttcacttt gagcataaat ttcctgctcc ataaaatatt
     2221 catattctgg aagtagctca acatcttcgg ctcgcaattt ggaatataca ggtttggtag
     2281 gaatttgatc gcctgttttc caaatttcat tcggttggtt aagccgttta aattttaaat
     2341 ccttttgggc atatacctga taattttctg cagtatattc aataaattcc ccttcgcgaa
     2401 gatttaccaa ttgcttagta aaacgaagta cagcagtaag atctgaagat gctaatgaaa
     2461 atggcatccc ttctatagtg cctatgccaa aatataaact actaccagct ttaattgccc
     2521 aactggtttc tgtatctgga tctacaatta ctgcagcata acttcctact aacttatttg
     2581 cagcttgaat aattgcttgc ctcatacaat ttttacgctc ttccggggct tttggatttc
     2641 cagttttatc catttcgata tcgaaataat gttcaattat atgaactaac atctctccat
     2701 cattatcact ttgtacaaaa tgaccttgat taagcaagaa tgactttaat tctcccgtat
     2761 tagtgatatt gccattatgt gcaccataaa tatgacgctt gcaattaact tcgtgaggct
     2821 gagcattaat cttatctaca aaaccgaatg tcgcccaacg tacttgccca cagaatattt
     2881 ttccactctg tttttcgatt cctaaggtct taactaaagt gctgggagcc cctacatcct
     2941 ttaaaagggt tatttctgtc gtgtctccct gaaatgctgc ccctgtgcta tcataacctc
     3001 tatattctaa tgattttaaa agcttggaag cgtaaacccc catattcaat gaaaccttgg
     3061 gtagtgctaa acctaaaaca ccacaaccaa aaccaaaaac aggaatcgga atacgcaatt
     3121 cccatttcct aataatagat aataatcgct ttagcaaaga atgcaaaaaa aaccacatcg
     3181 ttatgtcctc tatattttat ttttctctat tagctgctta gcaaaaaaaa gatctgcttc
     3241 atcagtaatt ttgatattaa aatcgtcact ccaaagaaca cgaattctct ctcccaaatt
     3301 ttgaatcaga gaagcatcat cggtagaata aaaattattc ctatatgccg caatatatgc
     3361 ttccataata agtgcatact caaaaacttg aggagtgaat acatttacta actggttccg
     3421 attaatagtt ctaactgcat aattatcttc tatttgcata actgtatgtt ttagcttgct
     3481 tgctggaaca actgctttat caacagttac tgcagcaaaa agaagttcga tcaattccaa
     3541 agtaacaaaa ggcctaactc catcatgtat agcaacatat tcagttcctt cagggcaagc
     3601 cttaagagcc gaaaaaacag aatcttgacg ttctgctcct cctgggatga ttttataggg
     3661 cttggtaaat ccttcaaacc attgttcaat aagggcttcc gttttggtaa tatcttcttc
     3721 aggaacagta actattatat tatcaatcaa ttccgaagcc ataaaaggtt ccatacttct
     3781 tatcaaaata ggaattccta ccagctctcg aaattgcttt ttactgctac ccgggaggcg
     3841 tttaccactt ccagccgcag ttataattgc ggttgtattt ttcaggaagt ccatatataa
     3901 aaaacacctc tactttctct tttaacggta atttttttct ccaaacacaa ttgccgcagc
     3961 actttactta tttcgttaat atgaatatct aacattgcgg aaagatcttc ggcagtggat
     4021 ggtcgcctta caagtgtact atgcagcatt tcaagtagtt ctgtatccaa ttgactttca
     4081 gtagttttat attttacttt ggcaataatc tctactggta cagaggtctt ttctttaaac
     4141 tgagctttaa tctgcttgag gttatgtaat ggtataggat taacccaatc ctctgctcct
     4201 ggacgatcca aacaatttag ctgcaccaaa tttggtttaa ttctatttat tgctttggca
     4261 agtgcttcga tttcttcttc tgtatcatta attcctttaa tcagaaaaac ctccagccaa
     4321 atagtaccct tatattcttt tctgaattga actaaaccct ctatcaaatc ctgtactttt
     4381 agttcgggac agggacgatt gattttttca aaaccttctt gcgtagcaga atcaagcgaa
     4441 ggcaaaacaa tatcacaggg taaaatttct tggcgaactt ccttatcgtt aaaaaggact
     4501 ccattggtta gaagtgctaa tttataactt ggatatttag cttttatata gtttactatt
     4561 tctccgatgg aactatttaa agttggttct ccagctcctg aaaatgtaat ataatccaga
     4621 tgtggtgcag ttgccataaa attatcaagt tcatctaata tctccctaac ttcgaaatat
     4681 gattcacgct tggttacttg atgtgtagtt ttctgcactt cacaataaac gcaatttaaa
     4741 ggacagtatt tatatgggac aaggtctatg cccaaagaaa tacctaagcg acgggacatt
     4801 acaggtccga aaaggtgttt atacttaatc atcttaaaat gtggttccaa attgtaaatg
     4861 cggt
//
LOCUS       DAORHB010000108         4841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_18388_length_4841_cov_6.450063, whole genome shotgun sequence.
ACCESSION   DAORHB010000108 DAORHB010000000
VERSION     DAORHB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18388_length_4841_cov_6.450063"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKM80_04045"
     CDS             <1..826
                     /locus_tag="PKM80_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNV93113.1"
                     /translation="FTKNGFAGEFEYSDFGHSMTLKKEEVETKVSYEAKKFADFNLKD
                     LCDKLQHQATTLAVKQNFEPQKIAVILRPVALQELLWFMGWMMDRRQSDEGFTPFTDK
                     IGKPFFGDKFSWYSTMQQPQLLALPYSSDGIISEEIPWIEKGILKNLPVSRYWAKKIN
                     SKPSSAYNMYIPGEGYTEEEMMKMVPRGLIVNTFWYIRSVDTKAGEFTGTTRDGVWYF
                     EDGKIKYAVNNLRFNEIPHDATRRIISTGKSELASPSSLLPAMLIDNFNFVDRTTF"
     gene            967..1140
                     /locus_tag="PKM80_04050"
     CDS             967..1140
                     /locus_tag="PKM80_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNV93114.1"
                     /translation="MPDRTLICQDCSKEFVFTEGEQEFYKEKGLQNEPKRCPDCRKAK
                     KAQFNRNRRRQSY"
     gene            complement(1451..3448)
                     /locus_tag="PKM80_04055"
     CDS             complement(1451..3448)
                     /locus_tag="PKM80_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93115.1"
                     /translation="MLKYTLIVLFSLLISGFLNYQTNPEIEKKRRITLFVLRFFSISL
                     LLILLLSPILYYTQHKKQTAQILVLEDVSQSMNLKHNSGSKAQHLKPLWQKMEKKFAD
                     NDYEVIHKTFANGLNGDKNATLLAPALKELSNQKAFRNLEGVVLASDGWLRDESLSIV
                     QQLGCPFYVLADSTVNPTPDLAIVNVRYNRYAYKNEPNTIRAEISAQNYSGNAEVRLL
                     LANKIISRQNMKLEKGIISTVDFTQRFNNIGFFPWKVEVVPLPNESQINNNIFPGAIE
                     VLSEKQRIILISDKPAWDNKFTLDAIATNSRWEAESYLNRNGRLFSGEVLVNELDSHN
                     LAAIIIINNGMLNLDNSTANFIQNAFQRGVGIMYQGLPVNELSAILPIQKSNITSIYQ
                     GFLVPTAAAANYPMLNSLTVNTKDIPPLDYYYVTASPGTDILATINNPQNSPAIVVQH
                     TKTARSIAFATLNLWRWQMQSSEEGYTKLISSCLTWLSNKSTGNYTAIYNNSYFQGEE
                     IRIRLRNENEIRQSRLNVNPRIRIMNKNNKEVYTDFLKREGEEYAISLQLSEPGTYSF
                     EISDKESGNSTKGKFELSETSIETRDYGYNLPLLSWLANETGGKVLYASNIDSYNPTP
                     AKPIEQITRKEIPLYKKWYVIALFIFIFCLELFFRRRWGLL"
     gene            complement(3448..4698)
                     /locus_tag="PKM80_04060"
     CDS             complement(3448..4698)
                     /locus_tag="PKM80_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNV93116.1"
                     /translation="MKRCILLVLIFLISVSVLNAFNTGKSVLFADSYMLRAKGVEAAY
                     WNPANLKQVKGIDFWLPIMNLGISVNNNSLDLDTYNYVVSQDFLDDEDKIKILNKIDG
                     SLRSAVEGNISIIGFAFNNISFSSSLHLTGRLAVSERFVDLLLYGNTDSLYVFDKSTT
                     DISGLSYADFTFGMGNFIIPFIPEPIPKIRAGFSVSALVGVGDANTQEFNGYFNSSME
                     GLSLQQDISLHTGIGGYGQKVMLGLASNVTPQLEVGLTLDNLFGKITWPFTTEARNIS
                     ITADDVYASNIDEEFYDYTSETVDIDTYSTELPPELRLGALYSMKRANLSVDYLQGFK
                     SSINNSNIGRISMAAQVLPLKFLPIGMGVCFGNSIYPWRVSYSIGLHTSTGEIGFGVQ
                     SIKQLFPSYKSKGLSIGSYIKVNI"
BASE COUNT         1540 a          901 c          911 g         1489 t
ORIGIN      
        1 cttcacgaaa aatggttttg ccggtgaatt tgagtatagc gattttggtc attctatgac
       61 cctaaaaaag gaagaggtgg aaacgaaggt ctcttatgaa gccaagaaat tcgctgattt
      121 taatttgaaa gacctttgtg ataaattgca acatcaggca actacccttg ccgttaaaca
      181 aaactttgag ccacaaaaga ttgccgttat tttgcgtcct gttgcgttac aagaattact
      241 gtggtttatg ggttggatga tggaccgccg ccagtctgat gaaggtttta caccttttac
      301 ggataaaatc ggtaaaccct tcttcgggga taaattcagc tggtattcaa caatgcaaca
      361 gccacaactt ttagctttgc catattcttc cgatgggatt atctctgaag aaattccctg
      421 gatagaaaaa ggaatattaa aaaatcttcc tgttagccgc tattgggcaa aaaaaataaa
      481 cagtaaaccc agcagcgcat ataatatgta tattccgggt gagggataca ctgaagaaga
      541 aatgatgaaa atggtcccgc gtggacttat tgtaaatact ttttggtata ttcgcagcgt
      601 ggatacaaaa gcaggtgaat ttaccggaac aacgcgtgat ggagtttggt attttgaaga
      661 cggaaaaatt aagtatgcag taaacaattt acgctttaat gaaatccctc acgatgctac
      721 gcgtagaatt atttccacag gcaaaagtga gcttgcttcg ccaagttcgc tgctcccggc
      781 tatgctgata gataacttta attttgtgga tagaacaact ttttaaagaa atgaattgac
      841 aaatttttga aaactaaaaa taggtgttac taaatgcgat tcaacattca ccctaagcca
      901 gttccttttg tttcgggagt gcctttggac cagaatgttc gatcccgtta ataaaaggag
      961 ataccaatgc cagacagaac acttatttgc caggactgct ccaaagaatt tgtcttcacc
     1021 gaaggtgaac aagaattcta caaggaaaaa ggccttcaaa atgagcccaa acgctgccct
     1081 gattgccgta aagcaaaaaa ggctcagttt aatcgtaaca gacgcagaca atcttactaa
     1141 gaaagtttat taatctgtta acgccactgc tattttgcgg tggcgttttt gtttgccttt
     1201 tactgataaa aataaatttc acgcagatta cgcggattac gcagattttt gagaaaagag
     1261 tattcaggga gataggagaa atcagagtga atagaggtta gggcagtaag tattgttgga
     1321 taggtctgca tttatcagct ttgcagttat aaacagcaca caaaagaaag attcaggggc
     1381 tccagaaatc cccagcgccc attttcaatt tattgcttca gactcttgct atcttgttcc
     1441 ccttttaaag ttatagcaat ccccatcttc ttctgaaaaa caattccaag caaaaaatga
     1501 aaatgaagag agcgataaca taccattttt tatagagggg tatttcttta cgggtaattt
     1561 gttcaatcgg tttagcgggg gtaggattat aggaatcaat atttgatgcg tacagtactt
     1621 tccctccagt ttcatttgcc agccaagaga gtaagggcag gttatatcca taatctcttg
     1681 tttcaattga agtttctgaa agttcgaatt tacctttcgt gctatttccg ctttctttat
     1741 ctgaaatttc aaagctgtaa gttcccggtt cagagagttg cagagaaata gcatattcct
     1801 caccttcgcg ttttaagaaa tcggtatata cttctttgtt gtttttattc ataatccgaa
     1861 tgcggggatt tacatttaga cggctctgtc taatttcatt ttcattgcgc aagcgaatcc
     1921 gtatttcttc tccctgaaaa tagctattgt tataaatggc agtatagttt ccggtacttt
     1981 tattgctaag ccaagttaag caactgctga ttaattttgt gtatccttct tcactgcttt
     2041 gcatttgcca acgccaaagg tttaaagtag caaaagctat cgagcgggct gtttttgtat
     2101 gttgaacaac gatggcaggt gaattttggg ggttattgat agttgccaaa atatcagttc
     2161 ccgggctggc tgttacataa taataatcca agggaggaat atctttggta tttacagtta
     2221 agctattcag catcggataa ttagcagctg ctgcagtagg tactaaaaaa ccctgataaa
     2281 tcgatgtaat attagatttt tgaataggta aaatagctga cagttcgtta acggggagtc
     2341 cttgatacat aatacccacg cctcgttgga aggcattttg gatgaaatta gctgtagaat
     2401 tatccaagtt tagcatacca ttattgataa tgattatagc agctaagtta tgggaatcca
     2461 attcattaac taaaacttct ccgctaaaga gtcgtccatt tctatttaaa taactttcag
     2521 cttcccagcg ggaatttgta gctatggcat cgagggtgaa tttattatcc caagcaggtt
     2581 tatccgaaat aaggataatt ctctgttttt ccgatagcac ttcaattgca ccggggaaaa
     2641 tattattgtt aatttggctt tcgttaggta ggggcacaac ttctaccttc caagggaaaa
     2701 aaccaatatt attgaagcgc tgggtgaaat ctactgtgct gattatccct ttttccagtt
     2761 tcatattttg tcgtgagata attttattag ccagcaacag acgcacttct gcattgccgc
     2821 tataattttg tgcagaaatt tcagctctaa tggtattggg ctcattttta taagcatagc
     2881 gattgtagcg aacatttaca attgccaaat caggagtggg atttacagtg ctatctgcca
     2941 aaacataaaa agggcagccc aattgttgaa ctatagataa agattcatct cttaaccagc
     3001 catctgaggc aagaacaact ccctccagat ttcggaaagc tttttggttg ctcaattctt
     3061 ttagggcagg agccagaagg gtagcatttt tatctccgtt caaaccgttg gcaaaagttt
     3121 tatgaataac ttcatagtcg ttatctgcga attttttctc cattttctgc caaagaggtt
     3181 ttaagtgttg agccttggaa ccagaattat gtttcagatt catactttgg gaaacatctt
     3241 ccagcaccaa aatttgagct gtctgttttt tatgctgagt ataatataaa attgggctaa
     3301 gtaacagaat caacaacaga gatatggaaa aaaacctgag gacaaaaaga gttatcctcc
     3361 gttttttttc aatttccgga ttggtttgat agtttaaaaa accagatatc agcagcgaga
     3421 acagaacgat cagagtgtat ttcagcatta aatattgacc ttgatatagc ttcctataga
     3481 taatccttta gatttataac ttggaaaaag ctgtttaata ctctgcaccc caaaaccgat
     3541 ttcaccggtg cttgtatgca aaccaattga ataactaact cgccagggat atatgctatt
     3601 accaaaacac actcccatac cgatgggtaa aaactttaaa ggcaagacct gagcagccat
     3661 agaaatacgg cctatattgc tattgtttat tgagctttta aaaccctgca aatagtctac
     3721 tgataaatta gctcttttca ttgaataaag agctcccaac cgaagttctg gaggtagctc
     3781 tgtagaatat gtatcaatat ctacggtttc agaggtataa tcataaaatt cctcatctat
     3841 attggaagca taaacatcat ctgctgtaat actaatattt cttgcttcgg ttgtaaaagg
     3901 ccaggttatt tttccaaata ggttatccaa ggtaaggcct acttctaatt ggggagtaac
     3961 attactggcc agaccaagca taactttttg accatagcca ccaatacctg tatgtaaaga
     4021 aatatcctgt tgtaaagata agccttccat agaagaatta aaataaccat taaattcttg
     4081 tgtattagca tcaccaacac ctactaaagc gctcacggaa aagccagcgc gaatttttgg
     4141 tatcggttca ggaataaaag gtatgataaa attacccatt ccaaaagtga agtcagcata
     4201 gctcaaaccg ctaatatcgg tagtgctttt atcaaaaaca tataatgaat cggtattacc
     4261 ataaagcaat aggtccacaa atctttccga tactgccagc cttccagtta aatgaagaga
     4321 actagaaaag gaaatattat taaaagcaaa accaataata ctgatattac cttctacagc
     4381 tgaacgcaga gaaccatcaa ttttgttcaa tattttaatt ttatcttcat catctaagaa
     4441 atcttggctt actacataat tataggtatc cagatccaga gagttattat ttacggatat
     4501 tcccaggttc ataatcggta gccaaaaatc aatacccttt acttgtttta aattagcggg
     4561 attccaatag gcagcttcta cacctttggc acgcagcata tagctatcag caaacaggac
     4621 agatttacct gtattaaatg catttaaaac actaacagat atcagaaaga taagcactaa
     4681 taatatacac cttttcattt caatcctcct ctattagtat ttcggcagag atcattcctt
     4741 taagatgaat ataatctcca ctgctggcgt gaatagtaac aggagttcct gtttcttcaa
     4801 aactgaaaga ccaccgcata taaacttgtt ctgaagcaaa a
//
LOCUS       DAORHB010000109         4820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_18514_length_4820_cov_3.427282, whole genome shotgun sequence.
ACCESSION   DAORHB010000109 DAORHB010000000
VERSION     DAORHB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18514_length_4820_cov_3.427282"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="PKM80_04065"
     CDS             <1..1214
                     /locus_tag="PKM80_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93117.1"
                     /translation="AVAYKALNYDNPVFSKCNFLPAKLLEEQKELKVVWHGFIVLTLI
                     FGVVMYSTIHYLTLNRSISNYKRANQEMEFTFNRLKAENAILEQIRSEIESYKENINR
                     IGTILQGKNRWTEMFNVLNTGFASQPSSWISNLKKADKEDRIVITGTTTRRENIAKIA
                     NSLPNSRISKVTNADIRKKKVWNFEMDFDLPEVDWVHDIENEFIQENPEVVERIIAKP
                     KSKSKSVRTSVKPVITNGTLSKAVSTKSNGIGINYNYGVLPLINSDYLPQPIGNELAN
                     NRKLASEYQNFLSSINKVNMLEYRFLGQKILAKYEDSSILPLIRWWIAYRLYCDKDYR
                     TASMYLEPNLRTRDYYYPYSVLLQARIDFASENDRFQQLYDSIISQYPDSDVYKQASI
                     DLKYIREGVLR"
     gene            1211..2080
                     /locus_tag="PKM80_04070"
     CDS             1211..2080
                     /locus_tag="PKM80_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93118.1"
                     /translation="MNNTARNTLVLASLLIVTGAVSYAISGRKHSKLNELKTKNKELT
                     QQIANIKNMMENKEELEQERELQIALASQRSKAIIDIDTPTITYDYLLSLLRWMNKDI
                     IYDFALSGKADSKEASQGTYNEYVLSGKCNYLDFVDFTRNLEYQRAVITVEDISLAYT
                     AASNDTIDFSMIFRTHYQQGGVPISELSAKKIDKYVTAYQLFRPRVYETPIDYDFDTR
                     LLDIDNSTLIAITGSKAFIRDSKGLIRILSPGDRVLWGYLYQVYSKEGKAIFRVNKYG
                     YEEDQILTISKER"
     gene            2092..>2639
                     /locus_tag="PKM80_04075"
     CDS             2092..>2639
                     /locus_tag="PKM80_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93119.1"
                     /translation="MKKSIILVCLILAISITGLLAQTQKEMEKLTLSKDIRINEALQI
                     LEPFFIRDSGKKLINLSSYNGPLGVPINNLDYKEALELILLKNNLIQTEAVGYIAIQD
                     KAATDLGIGVETGKDSIMPTAEAVQLPEGVSPKDKQVLINAVIMLVDKSYSKSLGIDW
                     STLVDGKVKVNAGFSGASQVTAP"
     assembly_gap    2640..2649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2650..3249
                     /locus_tag="PKM80_04080"
     CDS             <2650..3249
                     /locus_tag="PKM80_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93120.1"
                     /translation="MNLSAETSTEAGNYTIDINTLLKTIETEQKGSILAKPNIRVSNA
                     RKGHIQVGQDISIKRVDEAGNTVESFFSTGVIMDVEPTIIKVDDEEVIYLKLNIERSS
                     GVPSDVSTIITKSTSSTEVILFSNEEIAIAGMFDTDETTVRSGIPILKDLPWWVFGIR
                     YLTGYNTVEKKERELVITIKAEIVDNAIDRLLESRSTHM"
     gene            3273..>4820
                     /locus_tag="PKM80_04085"
     CDS             3273..>4820
                     /locus_tag="PKM80_04085"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV93121.1"
                     /translation="MKISQRLLLLFIVIAIVFGAFFYLFNYIKQEEMKVYIEYDNNQQ
                     RLALNALIATKSDQQMRFLDEFSINDDIYSAVASHNVTKAEKLLTPLISSFGYSVAQI
                     YDEQGHLLLNLYSESLPELNNLLIDSIVLDSLKTGKAMQYFHRLQHLVLENAISSIHS
                     SADTMREDKIAGYLLIAQVWDQKRLSEFTKLLDFNANISLSKPIASDEDKATQYRTTI
                     TFPLNDLNGYEIAWLTFTRSNPYLRQLRSLGNLILFGTLGFIFIFLLVHFILIHQWIS
                     SPLQLISNSLSDNDPEYIKPLLDKNNEFAAIALLIQNFFVQKDQLIQEIEERIRTEAK
                     LKEAEEQTRKILLTSPEAIIVTDIRGKVLSINAEGLKILGVENEESVLFNPISAKDFV
                     ARWELRALYQMLAKLRKGIPVKNLEITFDNGKGQSFPSLVSASVITDNEGKPNRFIFI
                     ARDITDLKNLERQLRQSQKMESIGTLAGGIAHDFNNIITIIAGYIALARGKISDPQAS
                     EADLNQAI"
BASE COUNT         1636 a          836 c          928 g         1410 t
ORIGIN      
        1 tagctgttgc ttacaaagct ctaaattatg ataaccccgt ctttagtaaa tgcaattttc
       61 tacctgcaaa actattggaa gaacaaaaag agctgaaggt tgtttggcat ggttttattg
      121 ttctaactct tattttcggg gtggtaatgt actccactat tcattacctt acactaaacc
      181 gaagcatcag caactataaa agagctaatc aggaaatgga atttactttt aaccgcctga
      241 aagcagagaa tgcaattttg gaacaaataa gatcagaaat tgaaagctac aaggaaaaca
      301 taaaccgtat cggaactatt ctgcagggaa agaatcgctg gacagaaatg ttcaatgttc
      361 tcaataccgg ttttgccagc caaccttcaa gctggatatc aaatttgaaa aaagccgata
      421 aggaagatag aattgtaatt actggcacta ctactcgacg agaaaatata gcaaagattg
      481 ctaattcgtt acctaattcc aggatcagta aagttaccaa tgctgatatc agaaagaaga
      541 aggtttggaa tttcgagatg gattttgatc ttccggaagt ggattgggtg cacgatattg
      601 aaaatgaatt tattcaggaa aacccagaag ttgtagaaag aataatagcc aagcccaaat
      661 ccaaatccaa atccgttaga acatctgtaa aaccggttat cacaaatggc acactaagca
      721 aagcggtatc aacaaaatcg aacggcatag gaatcaacta taattatggt gttttaccgc
      781 ttattaattc ggattatcta ccacaaccta ttggaaatga gcttgccaat aatcgtaagt
      841 tagcaagtga atatcagaat ttcttaagct ccatcaataa agtaaatatg ttggaatacc
      901 gtttcttggg acagaagatc ttagctaagt acgaagatag ttctatacta cctttaattc
      961 gctggtggat tgcttatcgc ctctattgtg ataaggatta tagaactgct tcaatgtatc
     1021 tggaaccaaa tcttcgaacc agagattatt actatcctta cagtgtattg ttgcaagcac
     1081 gcattgattt cgccagcgaa aatgatcgct ttcaacaact ttatgattcc attatttccc
     1141 aatatccaga ttcagatgta tataagcagg caagtataga tctcaaatac atccgtgaag
     1201 gagtgttaag atgaataata ctgcacgcaa cactttagtt ttggcaagtt tactaatcgt
     1261 tacgggtgcc gtttcttatg ccataagtgg cagaaaacat agcaagctga atgaactaaa
     1321 aactaagaat aaagaactta cacaacagat tgctaacata aaaaatatga tggaaaataa
     1381 agaagagttg gaacaagaga gagagcttca aatagctctt gccagtcagc gcagtaaagc
     1441 aattatcgat atcgatactc caactataac ttatgattat ctattaagtt tgctgaggtg
     1501 gatgaataaa gatattatct atgattttgc tctctccggt aaagctgata gcaaggaagc
     1561 cagccaggga acttacaatg aatatgtcct ttctggaaag tgtaattatc tggattttgt
     1621 ggattttact cgcaatttag aatatcaaag agctgtgatt actgtggagg atatctcgct
     1681 ggcttatacc gctgcaagta atgatacgat agatttttct atgattttta gaactcatta
     1741 ccaacaggga ggagttccta taagcgagct aagcgctaaa aagatagata aatatgtaac
     1801 tgcatatcaa ttgtttaggc ctcgcgttta tgaaacacct attgattatg attttgacac
     1861 tcgtttactg gatatcgata atagcaccct tatcgctata acaggatcaa aggcatttat
     1921 tcgtgatagt aaaggtctaa ttcgtatcct cagcccaggc gatagagttt tgtggggcta
     1981 tttatatcaa gtttatagta aagagggaaa agcaattttc agagtaaata aatatggcta
     2041 tgaagaagat cagattttaa caataagcaa agaaagatga ggttagccca aatgaagaaa
     2101 tctattatat tagtttgcct aatactggca attagcatta caggcctgct tgcacaaacc
     2161 caaaaagaaa tggaaaaatt aacattgagt aaagatatcc gcattaatga agcgttgcag
     2221 attttggaac ctttttttat tagggattct gggaaaaagc taattaacct ctcttcctat
     2281 aatgggccct taggtgttcc cattaataat ctggattata aagaagcgct tgagctaatt
     2341 ttgctgaaaa acaatcttat tcaaactgaa gctgttggtt atattgctat tcaagataaa
     2401 gctgcaaccg atttgggaat tggagttgaa acgggaaaag atagtataat gccaactgct
     2461 gaagctgttc aattaccaga gggagttagc cccaaagata aacaggtctt aattaatgcc
     2521 gttataatgc tggtagataa aagctattcc aaatcattag gtattgactg gtcaaccctg
     2581 gtagatggca aagtaaaagt aaatgctgga ttttcagggg catcgcaagt aaccgctccn
     2641 nnnnnnnnna tgaatctttc cgcagaaaca tccactgaag ctggtaatta tactatagat
     2701 ataaatactc tgctaaagac tatagaaacg gaacaaaaag gaagcattct tgccaaacct
     2761 aatatcagag tttccaatgc ccgaaaaggg catatacaag ttggccagga tatttccatc
     2821 aagcgagttg atgaagctgg aaatacagtg gaatcttttt tctctacagg ggtaataatg
     2881 gatgtggagc ctactattat caaagtggat gatgaagaag taatttacct gaaacttaat
     2941 attgaacgct ccagtggagt tcctagtgat gtttccacca tcattaccaa aagcacttcc
     3001 tctacagaag taatattgtt cagtaatgaa gaaattgcca tagctggtat gtttgatact
     3061 gacgaaacaa cagtccgcag tggcattccc atcttgaaag acctcccttg gtgggttttt
     3121 ggcatccgct atctaactgg ctataacaca gttgagaaaa aagaaagaga gctagtcata
     3181 accatcaaag ctgagattgt agataatgct atagacaggt tactggaatc gcgatctaca
     3241 catatgtaaa atgataatat aaggaagatt cagtgaaaat atctcaacgt ttattactgc
     3301 tttttattgt aattgctata gtttttggag cattcttcta tctgttcaat tatatcaaac
     3361 aggaagaaat gaaggtttat attgaatatg ataacaatca acaacgcctt gctcttaatg
     3421 ccctaattgc tacaaaaagt gatcagcaga tgcggttttt agatgaattt agcataaatg
     3481 acgatatcta ttctgctgtt gcttcgcaca atgtaacaaa agcagaaaaa ttacttactc
     3541 ctctaatcag ctcctttggt tatagtgtag ctcaaatcta cgacgagcag gggcatcttt
     3601 tattgaatct atatagcgaa tcacttcccg aattaaacaa tttgctgata gattccattg
     3661 tgcttgattc tctcaaaaca ggtaaagcaa tgcagtattt tcaccggttg cagcatttag
     3721 ttttagaaaa cgctatcagt agtattcata gttctgctga cacaatgaga gaagataaga
     3781 tagcaggata tcttcttatt gctcaggttt gggatcaaaa gagattaagt gaatttacaa
     3841 agctgctcga ttttaatgca aacataagct taagcaaacc tattgcttct gatgaggata
     3901 aagcaactca atacagaact actattacct tccctttgaa tgatctgaac ggatatgaaa
     3961 tcgcctggtt aactttcacg cgttccaatc cttacctaag acaattgcgc tctttgggaa
     4021 atttgatcct ctttggaact ttgggattta tctttatttt cttattggtt catttcatcc
     4081 ttattcatca gtggatcagt tctcctttac aattgatatc caattcttta agcgataatg
     4141 atcctgaata tattaagccc ctgttagata aaaacaatga attcgcggct atagcgttat
     4201 tgatacagaa tttcttcgtt cagaaggatc aacttatcca agaaattgaa gaacgcattc
     4261 gcacagaagc caaacttaaa gaagcagaag aacaaaccag aaaaatactg ttgacctcac
     4321 ctgaggccat aatcgttaca gatataagag gcaaggtttt aagcattaat gccgaagggc
     4381 tgaaaattct tggcgtcgaa aatgaagaga gtgttttgtt taatcctata tctgctaagg
     4441 actttgttgc gcgttgggaa ttacgtgctt tataccaaat gctggcaaaa ttacgcaaag
     4501 ggattccggt taaaaatttg gaaataactt tcgataacgg aaaggggcaa agtttcccca
     4561 gtttagtaag cgcttctgta attacagaca atgaaggaaa acccaataga tttattttta
     4621 tcgctcgaga tatcacagac ctaaaaaact tggaacgcca attacggcaa tcacagaaga
     4681 tggaatctat cggaactttg gctgggggca tagctcatga tttcaataat attatcacaa
     4741 ttattgctgg gtatattgct ttagcaagag gcaaaattag cgatccgcaa gcctctgaag
     4801 ctgatctgaa tcaggcaatt
//
LOCUS       DAORHB010000110         4726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_19072_length_4726_cov_4.494113, whole genome shotgun sequence.
ACCESSION   DAORHB010000110 DAORHB010000000
VERSION     DAORHB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19072_length_4726_cov_4.494113"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKM80_04090"
     CDS             <1..552
                     /locus_tag="PKM80_04090"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV93122.1"
                     /translation="IFIIIPFWTNLIIRIFSWRIFLAYNGVLNNILLNLGLISQPLEI
                     MRTDIAVILVMVYVYLPYMILPLYSVLEKLDWTLLNAAMDLGANEIISFFKITLPLAR
                     GGIFAGGLLVFIPALGSYLIPQLVGNQKSLYLGQVITYKVKNIPRNWPMASALSLILL
                     LIVALFLIMFYVLYSHSKSRRRV"
     gene            549..1349
                     /locus_tag="PKM80_04095"
     CDS             549..1349
                     /locus_tag="PKM80_04095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV93123.1"
                     /translation="MKRFSLTKLFKGLNLTSFTLVMVFLYIPILILIIFSFNDAKIIT
                     GWKGFTLKYYAQLLKNETIQIAFRNTMLIACLSTLISTILGVLTALGLENKKFSGSKF
                     VSILIYIPLVIPDILMGVSLALLFNFTKVNTGMLTVLIAHITFCISYTIIVIRSRLDD
                     FDYSLVEAAMDLGAKPSTIFWKINIPLILPGIIAAALLAFTLSIDDFIITFFTSGRGF
                     DTLPIYVEGAIRRGTITTINSLSTIMIGLTLLLVVTTKRIRKIVISSL"
     gene            1570..2508
                     /locus_tag="PKM80_04100"
     CDS             1570..2508
                     /locus_tag="PKM80_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93124.1"
                     /translation="MNEIKIDKNSPNQFDTLPKGWVFYLLSGLNEELYAGYSANLKVK
                     LNYLYHKAMEGGIYQDMWNSVEKLNWVTYDRAISALIHCKCYLAENMPLYQYQIRPWE
                     EYVYLSLNSHRYPFIAIQEHTSDDWLYLGPFRNRFFLVDVLDIFSRILKLPACETGEY
                     PCDKWEQNLCQGWCLALADNAKINPEYSLDKLESLLKETFLVPENGVLELVQQERDRY
                     FNNLEFTKASLLNDEIELLSKYRNWLKFLIAAKHLNYNSEDLEIQKGKLTFCNFQGRK
                     YHFPIDNTEFRINETLALNLDTVDESKIIYDFLKLT"
     gene            2520..3401
                     /locus_tag="PKM80_04105"
     CDS             2520..3401
                     /locus_tag="PKM80_04105"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNV93125.1"
                     /translation="MHNEISKLAIVKESHQGKVRDIYDLGENLLIVSTDRISAFDVVF
                     PDPIPDKGKILNAISAHFFKTTSNIVPNHFITDNVSDYPNELQKFKEYLDGRSMLVKK
                     TRVIPFECIVRGYISGSAWKEYKKTNTIGGMLIAEDMQESQKFTHPLFTPSTKVEDGH
                     DENISYREFMNRMDELLAVYIKDKSLELYSDAHDLLLKKGVILADTKFEYGTIGSQVL
                     LIDEVFTPDSSRLWALENYEIGKSPDSFDKQFIRDYLENSGWNKQPPAPSLPTEIIEK
                     TRQKYVTILKIITGKEL"
     gene            3404..3775
                     /locus_tag="PKM80_04110"
     CDS             3404..3775
                     /locus_tag="PKM80_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV93126.1"
                     /translation="MKKIICIVDDEEELVENLKIELEHLKPEWNILTFSEGMKAIQEI
                     VKGTVDLVVTDIAMPDMDGYELFWRIKDYDENIPVIMMTGFGYDPNHVLVRAKVDGLK
                     DVLYKPFETEKLVELIESHLK"
     gene            3776..>4726
                     /gene="lpxK"
                     /locus_tag="PKM80_04115"
     CDS             3776..>4726
                     /gene="lpxK"
                     /locus_tag="PKM80_04115"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNV93127.1"
                     /translation="MDLNTFIQAHLLKRSPLSYALYPSGVLYGYIQKQCRTYSRRRAY
                     RAPCKIISIGNIISGGSGKTPLTISLANLLINKGLKVAVSHRGYKSAFENEPHIINAD
                     SGMLYDIHKTGDEAYLIASALLKVPVAVGRKRVLAVKLLLEKHPSLDVIILDDALQHN
                     YIHRDLDIISFDASIGLGNGFVIPAGYLREPLSNIPQNSLIIINRKNKAQTETLWLNK
                     INQLKIPIYHSSNTPKCFLDITGNVYPLDYLKEKRIVLVSGIANPASFADSVSSVGLS
                     YVKHYACIDHCDFKQPSLIENILELNPDYILCTQKDIMKLA"
BASE COUNT         1494 a          842 c          842 g         1548 t
ORIGIN      
        1 atttttatta ttatcccttt ctggactaac ctgattatcc gcatcttttc ctggcgtatc
       61 tttcttgcct ataatggcgt cttaaacaat atattattaa atttagggct tatctcacag
      121 ccactggaaa ttatgagaac agatattgca gttattttag tgatggtata tgtatattta
      181 ccctatatga ttttaccgct atacagcgtg ttagaaaaac ttgattggac tcttctgaat
      241 gccgcgatgg atttgggagc taatgaaatt atatcttttt ttaaaataac cttgccttta
      301 gccaggggcg gcatctttgc cggtggctta cttgttttta tcccagcttt aggttcatat
      361 ttaatacctc agctggttgg aaatcaaaaa tcgctttatt tgggacaggt tatcacctat
      421 aaagttaaaa acatacctcg caattggcca atggcttctg ctttatcact tattctactg
      481 ttgattgtag ccctatttct aattatgttc tatgtccttt attctcacag caaatctcgg
      541 aggcgagtgt gaaacgcttc tctttaacta agttatttaa aggtttgaat ttaacttcct
      601 tcactttagt aatggtgttt ctctatattc ccatcttgat cttgattatt ttcagtttca
      661 acgatgctaa aatcattact ggttggaagg gctttacttt aaaatattat gctcagttgc
      721 tgaaaaatga aactatccaa attgctttta gaaacacgat gctgatagct tgtctttcca
      781 ccttaatatc cactatttta ggagtgctaa ccgctttggg attggagaat aaaaagttca
      841 gcggaagtaa attcgtttcc attctaatct atattccttt agttatacct gatattttga
      901 tgggggtctc tttagcttta ctgtttaatt tcacaaaagt aaacacagga atgctgacag
      961 ttttaattgc tcatatcact ttttgcattt cttacacaat tattgtaatt cgcagtcgcc
     1021 ttgatgattt tgattattct ttagtagaag cagcaatgga tttgggagca aagccctcaa
     1081 ccatcttctg gaaaataaat ataccattaa tcttgccggg aattattgct gctgcccttc
     1141 tagcttttac tctttccata gatgatttta ttatcacttt tttcacttct ggaagaggtt
     1201 tcgatacttt accaatttat gtggaaggcg ctatccgccg gggaacaatt actaccatca
     1261 attccctatc cactattatg atcggtttaa ccttactgct ggtagttact acaaaacgca
     1321 tccgtaaaat cgttatttcc tctctataat cttctttttt aacacagtat tctctatcta
     1381 taagcgtttg gtttgataat gtagttttct gttacttgta tttccgaaat aggacacagt
     1441 attcattata atctatatgg gttcctgctt acaaaggaaa taacaatgag agaaaagaat
     1501 gtcagctaaa ggaagtaata tttcgggaat ttcgtttgac ataaacatca ggtaaacatt
     1561 tatggcaata tgaatgagat taaaatagac aaaaattcgc caaatcaatt tgatacctta
     1621 cctaagggtt gggtttttta tctgctttca ggtttgaatg aagaactata tgctggttat
     1681 tctgccaatc tgaaggttaa gttaaattat ctttaccata aagctatgga aggtggcatc
     1741 tatcaagata tgtggaattc tgtggaaaaa ttgaattggg ttacctacga tagggctatc
     1801 tctgcgttaa ttcactgtaa atgctatcta gcagaaaata tgccactata tcaatatcaa
     1861 attcgtcctt gggaagaata tgtataccta tcgttaaatt cacatcgcta tccttttatc
     1921 gcaattcagg aacatactag tgacgattgg ttgtatttag gaccttttcg taatcgcttt
     1981 ttcctggttg atgttttgga tattttttcg cgtatcttaa aattaccagc ttgtgaaaca
     2041 ggtgaatatc cttgtgacaa atgggaacag aatctttgcc agggttggtg tttggctctg
     2101 gcagataatg caaaaataaa tcctgagtat tccttggaca aattagaatc ccttttaaaa
     2161 gagacatttt tggtccctga aaatggtgtt ttggaacttg ttcaacagga aagagacaga
     2221 tattttaata acctggaatt tacaaaagca tcattgctaa atgacgagat agaactgcta
     2281 agcaaatacc gcaattggtt gaaattttta attgctgcca aacatttgaa ttacaatagt
     2341 gaagaccttg aaatacaaaa aggtaagctt actttttgca actttcaggg gcgaaaatat
     2401 cattttccta ttgacaatac agagtttaga attaacgaga cacttgctct taatctggat
     2461 accgtagatg aaagtaaaat tatctacgat ttcttaaaac tgacataagg agttttttta
     2521 tgcacaacga aataagcaaa ttagctatag taaaagaaag ccatcaaggc aaagtacgcg
     2581 atatatacga tttaggagaa aaccttttaa ttgtaagtac ggatcgaatc tccgcttttg
     2641 atgtagtttt tccagatcct atccccgata aaggaaaaat tctgaatgct atttctgctc
     2701 atttttttaa gactacttct aatatagttc caaatcattt tattactgat aatgtttctg
     2761 attaccctaa tgaactacag aagtttaaag aatatttaga cggacgcagt atgttagtaa
     2821 aaaaaacacg agtaattcct tttgagtgta ttgtgcgtgg atatattagt ggttctgcct
     2881 ggaaagaata taaaaaaact aatacaattg gagggatgct gattgcggaa gatatgcagg
     2941 aaagccaaaa attcactcac cccctcttca ctcccagtac caaagtagaa gatggtcacg
     3001 atgagaatat cagctacagg gaatttatga accgaatgga tgaattattg gctgtttata
     3061 taaaggataa atcactggag ttgtattctg acgctcacga tttactcctg aaaaaaggag
     3121 tgattttagc tgatactaaa tttgaatatg gaaccatcgg ctcgcaagtg ctattgattg
     3181 atgaagtttt cactccagat tcttcgcgtc tttgggcttt ggaaaattac gaaataggaa
     3241 aaagtcccga tagcttcgat aaacaattta tccgagacta cttggaaaat agcggatgga
     3301 ataaacaacc tcctgctcca tctcttccta cagaaataat tgaaaaaacc cgccaaaaat
     3361 atgtaactat cctaaaaatt atcacaggaa aagagctgta acaatgaaaa aaatcatctg
     3421 tatcgtagac gacgaagaag aattagttga aaacctgaaa atagaactgg aacacttaaa
     3481 accggaatgg aatatcctta cttttagcga aggaatgaaa gcaatccagg aaatcgtgaa
     3541 agggaccgta gatctcgtag ttacagatat tgctatgccc gatatggatg gctacgagct
     3601 gttttggaga attaaggatt acgatgaaaa tattccggtg attatgatga ccggctttgg
     3661 ttacgacccc aatcatgttt tagtacgcgc caaagttgac ggcttgaaag atgttctcta
     3721 taaacccttc gaaacagaaa aactggtgga acttattgaa tcccacttaa aataaatgga
     3781 tctcaataca ttcatccaag cccatttact aaagcgctct cctttgtctt atgctctata
     3841 tccttcggga gtactctatg gctatatcca aaaacaatgc agaacatatt ctcgccgaag
     3901 agcttatcgc gcaccttgca aaattattag tattggcaat atcattagtg gtggttcggg
     3961 aaaaactcca ctcacaattt ctttggctaa tttattgata aataaagggc taaaagtagc
     4021 tgtgtcccat cgtggctata aaagtgcttt tgaaaatgaa cctcatatta ttaacgccga
     4081 ttctggtatg ctctacgata ttcataaaac aggtgacgag gcatatttga tagcctctgc
     4141 cctacttaaa gttcctgtgg cagttggtag aaaacgggtg ttggctgtta aattactctt
     4201 agaaaaacat ccttcgctgg atgtgattat tttagacgat gccctacaac ataattatat
     4261 ccatagagac ttagatataa tcagttttga tgcttctatt ggactgggca acggctttgt
     4321 gattccagca ggatatttaa gagaaccact ttccaatatc cctcaaaaca gtttaataat
     4381 aattaaccgc aaaaataaag cacaaacaga aaccctttgg ctaaataaaa ttaaccaatt
     4441 gaaaatacca atctaccata gctctaatac tccaaaatgc tttttggaca ttaccggaaa
     4501 tgtttatccg ctcgattatc ttaaagaaaa acgaattgtg cttgtttccg gaattgccaa
     4561 tccagcttcc tttgcagatt ctgttagttc tgtgggacta tcttatgtaa agcactatgc
     4621 ttgtatagat cactgtgatt ttaaacagcc atccctaata gaaaacatct tggaactaaa
     4681 cccggattat attctctgca cccaaaaaga tattatgaaa ctggct
//
LOCUS       DAORHB010000111         4709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_19159_length_4709_cov_4.418994, whole genome shotgun sequence.
ACCESSION   DAORHB010000111 DAORHB010000000
VERSION     DAORHB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19159_length_4709_cov_4.418994"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2227)
                     /locus_tag="PKM80_04120"
     CDS             complement(<1..2227)
                     /locus_tag="PKM80_04120"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNV93128.1"
                     /translation="MLYGQKTQDFKPGRKCSFPQTYTNTEISRADSAHGFDVQKYEIT
                     LSINDAFHNVFGNVLATVQATENLSSIAYELKGLNVTSVKVNGINTNYTHSLGLITIP
                     LNVIAGEQFTTQIFYSGNPVLSNDAYHIGMIFSSNTVFTISDPDAARYWWPCYDHPWD
                     KAIVDLHITMRSDWKVASNGIRTAIVDNGDGTSTTHWIGQNPMTTYLVCITAGPYVEI
                     EQTVPEQNNLPIQNFVMQNQYNNALIDLQNLPLMISWFSELFGDYPFEKYGNAVVSMS
                     TYGAMEHQTMTTLGNYIITGTGYYETVIAHELVHQWFGNAVSFLTFKDVWLSEGFATY
                     SEFLWIDKTSGWQSACDYMRSSIQQYYLNWEIAEGPQTIYNPSFNNYFSPPSYEKAAS
                     VLHMLRLKLGDADFFALLQQYYNTYKNSNAITAEFQNIAEQVSGQNLSYFFNQWIYGS
                     GIPNIQYSLWQNEISNNLEIYAKTTSPTVTSFTVDIPFRISQNGISDSLLVIADPDGY
                     ANDYNYPIISDNMEVEANYNNWTLLNDLTEQIPRFTECLPSNDCVMLFWNSFISEKDY
                     QYQLYRKLSSEQEWTLISTMDNNTFSYADTTVSGGFNYDYRLRVLDINGYFSKFTDTI
                     TITPQSFSFANEFLVVDETKDGNGFTINPDDEMVDSFYSAALGGFACPIDNWDCATQG
                     LPDLATLSNYKLVLWHADDFSQNLLQDNLSVISSYIAGGGKLVLSGWKTASNLTTEFL
                     DR"
     gene            2471..>4709
                     /gene="bamA"
                     /locus_tag="PKM80_04125"
     CDS             2471..>4709
                     /gene="bamA"
                     /locus_tag="PKM80_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNV93129.1"
                     /translation="MQRFILCSILILLLGCFAQNLYAAGETIYEIKVIGAENIDPELV
                     TSALSIRVGDSLDSEAVAKSIRSLYRMGIFSDIQIETEPYRTGVTLLVKVQENPVVSS
                     IEYIGFKTIKKERIDELVSVRVGSYWSEGVKNQLLRTLKNEYASKGFSNAEIQIFESH
                     GAKNKIGLKVQVDEGKRVSIKQITFVGNNYFDGKTLQKKMKTKTSSFLRSGRLEQEKF
                     DADLQTLTAFYKKNGFIDVVIGPYEIQPLNDKAIEIVINIYEGTKYNFGGINIEGNEF
                     FPIEELKEAFTMKIGEPFDQEIFDNEMRIIYGKYFDEGFIYASIVPEYVKEGDQLIVN
                     IKINENNRARIRQIHITGNRRTKDKVIRRQLEVSPGDYFRQNKVIRTQQNIYNLGFFE
                     QDIRLDYTPVNSEGDIDLQFDVMDRASGTANGGVGYNSQDKFVGQLSVAQNNLFGNNW
                     STNLTWEFGGNTQTFEFAFTNPNLMDTDILLGTNIYYTKKTWSSFHYEIFTRGAGLRV
                     GQTVPWIDKTRAVIGYSLYSKKYRITNMDAIMADSSSNANLIELNKLSWRYTSATSLT
                     ISRDTRDNVFFPTKGSQFVIFSEIAGGMFGGDFDYFKQIAQVNWYMDTWEKLILRTKW
                     RFGYVTAYGRSKDAPPDEKFYLGGTGADGIRGFPDSSIGPSGGGTRAIIFSTELGYPL
                     GSDQIIAVAFFDAGNSYNKMRDFNFLNFKKGTGLGIRIRSPFGLIGFDYAYNINDGGW
                     EPHLQF"
BASE COUNT         1554 a          800 c          902 g         1453 t
ORIGIN      
        1 aacggtccaa gaattcagtg gttaaattag aagctgtttt ccacccagaa agaactaatt
       61 ttccacctcc agcgatatag ctactaatta cagatagatt atcttgcagc agattttggc
      121 tgaaatcgtc agcatgccaa agtacaagtt tatagttact taaagtagcc agatcaggta
      181 aaccttgagt tgcgcaatcc caattatcaa tcgggcaagc aaatccacct aaagctgcag
      241 aataaaagct atcaaccatc tcatcatcag gattaattgt aaagccattt ccatctttgg
      301 tttcatctac cactaagaac tcattggcga aactaaacga ttggggagtt atagtaatag
      361 tatcggtgaa tttagagaaa tagccattta tatctaatac ccgcaatcta taatcatagt
      421 tgaaaccgcc gcttacagtt gtatcagcat aactgaaggt gttgttatcc attgtggata
      481 ttaaagtcca ttcttgttca ctactcagtt tgcggtaaag ctgatactga tagtcctttt
      541 cagaaataaa tgaattccag aagagcataa cacaatcatt ggaaggcagg cattctgtaa
      601 atcgaggaat ttgttcagtt aagtcattta acaaagtcca attattataa tttgcctcaa
      661 cttccatatt atcgctaata attggataat tataatcatt agcataacca tccggatcgg
      721 caattaccag caaggaatcg ctaataccat tttgagagat ccggaaagga atgtctaccg
      781 taaaagaagt aactgtagga gaagtagttt tggcatatat ttccaggttg ttacttattt
      841 cattttgcca aagagaatat tgaatgttcg gaatgcctga gccgtaaatc cactggttga
      901 aaaaatagct caagttttga ccgctaactt gttctgctat attctgaaat tcggcagtaa
      961 tagcattact gtttttatag gtattataat attgctgtaa tagagcaaag aaatcggcat
     1021 ctccgagctt taatcttaac atatgtaaca cactagctgc tttttcataa gaaggtggag
     1081 aaaaatagtt attgaaagag ggattatata ttgtttgagg tccttctgca atttcccagt
     1141 tcagataata ttgttggatg ctactacgca tataatcaca agcactttgc caacctgagg
     1201 ttttatctat ccaaagaaat tcactataag tagcaaaacc ttctgaaagc caaacatctt
     1261 tgaaagttaa gaagcttaca gcatttccaa accattgatg aacgagttca tgagcaataa
     1321 ctgtttcata gtatccggtt cctgtaatga tataattccc aagagttgtc attgtttgat
     1381 gttccatagc tccgtatgta ctcatactta caacagcatt tccatacttt tcaaaaggat
     1441 aatccccgaa aagttcagaa aaccaggaaa tcatcagagg gagattttgg aggtctatta
     1501 gagcattgtt atattgattt tgcatcacaa agttttggat tggcagatta ttctgttctg
     1561 gaactgtttg ttctatttct acatagggac ctgcagtaat acaaaccaga taagtagtca
     1621 ttggattttg tccgatccaa tgagttgtgg aagttccatc tccattatct acaattgctg
     1681 tccttattcc gttagaagca actttccaat cactgcgcat agtaatatgt aaatctacaa
     1741 ttgccttatc ccaagggtga tcataacaag gccaccaata gcgtgctgcg tctggatcgg
     1801 aaattgtaaa gactgtatta gagctaaaaa tcatccctat atgataagca tcattggata
     1861 atacaggatt tcctgaataa aaaatctgag ttgtaaactg ctctccagct ataacattaa
     1921 gaggaatagt aatcaacccc aaagaatgag tatagtttgt attaattccg tttactttaa
     1981 cgctagttac atttaaacct ttaagttcat aagctatact ggaaaggttt tcagtagctt
     2041 gcaccgtggc taaaacatta ccaaatacat tgtgaaaagc atcgttaata gaaagcgtga
     2101 tttcatattt ttgcacatca aaaccgtgag cagaatcggc tctgcttatt tctgtgtttg
     2161 tatatgtttg ggggaaactg cattttctcc cgggcttgaa atcctgagtt ttttgcccgt
     2221 agagcataga taaacacata attaagccta gaataatcaa tatttttttc gctgatttca
     2281 ttattacaac ctcagtttac ataataaagc aatttatgaa taagacaaat agatttcttc
     2341 atactgaaac tactttaaaa aaacagcttt cctgtcaagg ccacagcttt tgttttaaaa
     2401 acataaaaaa atgtctttac taaaaagcgc tgttcacaaa gctgtctatt aactcttaag
     2461 tggaggaaaa atgcaaagat ttatactatg cagcatatta attctgctgc ttggctgttt
     2521 tgcccaaaac ctgtatgctg caggagaaac catatacgaa ataaaagtta tcggagcaga
     2581 aaatattgat ccggaactgg ttacttcagc tcttagtatt agagttggtg attctcttga
     2641 ttctgaagca gttgcaaaat cgattcgcag tctatatcga atgggtatat tttccgatat
     2701 tcaaatagag acggaaccct atcgaacggg agttacttta ttagtaaagg ttcaagaaaa
     2761 tccagttgtt agcagtatcg aatatatagg atttaaaact attaaaaagg aacgaattga
     2821 tgaattagta tcggttcggg taggtagtta ttggagcgaa ggagtaaaga atcaattact
     2881 acgcacactg aaaaatgaat atgcctccaa gggctttagt aatgccgaga tccagatttt
     2941 tgaatctcat ggagcaaaaa ataaaatcgg cttaaaagtt caggttgatg aagggaaaag
     3001 ggtatccatc aaacaaatca cctttgtggg aaataactat tttgacggaa aaaccctgca
     3061 gaaaaaaatg aaaaccaaaa cttccagctt tttgcgatcg ggacggttag agcaagaaaa
     3121 atttgatgct gatttacaaa ccttaactgc attctataag aaaaatggtt ttatcgatgt
     3181 agtaatcggt ccctatgaaa ttcaaccact aaatgataaa gccattgaaa ttgtaatcaa
     3241 tatatatgaa ggtactaaat ataattttgg ggggatcaac attgaaggaa atgaattttt
     3301 ccccatagaa gagctgaaag aggcatttac aatgaaaatt ggagagccct ttgatcagga
     3361 aatctttgat aatgaaatgc gcattattta tggcaaatac tttgatgagg gttttatcta
     3421 tgcttctatt gtgccagaat atgttaaaga aggagatcag ctaattgtaa atataaagat
     3481 aaatgaaaac aaccgtgcca gaattcgcca gatacatatt accggaaata gaagaaccaa
     3541 ggacaaagtt attcgtagac agttggaagt ttctccaggc gattatttcc gtcaaaataa
     3601 agtaatacgc actcagcaga acatttataa tcttggcttt tttgagcaag atattaggtt
     3661 ggattatacg cctgttaaca gtgaagggga tatagacctc caatttgatg ttatggatcg
     3721 tgccagcgga acagcaaatg ggggagtcgg ttataattca caagataaat ttgtaggtca
     3781 gctttccgtg gctcagaaca acctatttgg caataactgg tcaactaatt taacttggga
     3841 atttggaggt aacacccaaa cctttgaatt tgcctttact aatcctaatc tgatggatac
     3901 agacatcctt ctgggaacta atatatacta cacgaaaaag acctggagct ctttccacta
     3961 cgagattttt actcgaggtg caggtttaag ggttggtcaa actgttccct ggattgataa
     4021 aacccgagcg gttattggct attctctata ttccaagaaa tacaggatta caaatatgga
     4081 tgccattatg gcagattctt catccaatgc caatttaata gaattgaata aactcagctg
     4141 gagatatacc agtgctacca gtttaactat tagtagagat actcgtgata atgtattttt
     4201 cccaacaaaa ggcagtcaat ttgtgatttt tagtgaaata gcaggtggaa tgtttggtgg
     4261 tgatttcgat tattttaagc agatcgctca agtgaactgg tatatggata cctgggaaaa
     4321 gcttatttta cgaacaaaat ggcgttttgg ttatgtaact gcttatggta gatcaaaaga
     4381 tgctcctcct gatgagaaat tttacttggg cggaaccggt gcagatggaa ttcgtgggtt
     4441 tccagatagc tctataggac cctctggagg aggaacaaga gctataattt tttctactga
     4501 attaggttat cctttaggta gcgatcaaat tattgctgtg gccttttttg atgcaggtaa
     4561 cagttataat aaaatgcgcg atttcaattt tctgaatttt aaaaaaggta ctggtctcgg
     4621 aatcagaatt cgcagtcctt ttggtttaat tggctttgat tatgcctata acattaatga
     4681 tggcggttgg gaaccgcatt tacaatttg
//
LOCUS       DAORHB010000112         4692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_19260_length_4692_cov_4.103947, whole genome shotgun sequence.
ACCESSION   DAORHB010000112 DAORHB010000000
VERSION     DAORHB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19260_length_4692_cov_4.103947"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..657
                     /locus_tag="PKM80_04130"
     CDS             94..657
                     /locus_tag="PKM80_04130"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV93130.1"
                     /translation="MWKTDDRLLSLIFLQQHKLNMDIVDLDTSINLFLKDGIDIMNVM
                     RYNEYHQLQQAGIDIEDLFTVSFADIGLNVADNGLYTTREFYEKHSKHCRDFVDATIE
                     GWLYAINNQEETISTVLEIMHKHHLPANRPHQKWMLNELSQDFMVDSSQIGVLQKSQF
                     EEAKRLLTEQNMPITSQTYNSFYPYAK"
     gene            647..2560
                     /locus_tag="PKM80_04135"
     CDS             647..2560
                     /locus_tag="PKM80_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV93131.1"
                     /translation="MQSKQEERGLAYQIIVFIFFFLVLIFSVFIGITRFLFSEIMMDN
                     SRETVSHLAKETVYQIEGRLSAIVDISQSIITLYNSDYLNRNELDTILNDYIYEFDTL
                     ESITIAYAPEYNKSGDSRTIYHFKNKAISQELHPAEYQYLDWFQIPYELKSKCWTDPW
                     YDASSTKQTVISYCVPIFIKGNCIGVMRFDTKLSGLQRIVSPLKLKRSGYAFLVSSIG
                     TIITHPADSISFNESIFSLAEETKDTQLRELGKKMIQGETDFIRLRGNTIFNDSWIYF
                     SPLLSNNWSLGIVIAHKDVVRDLNLLLIIQTLFSVIIFISISLIVYYRTFSVSKPIRL
                     FTEVATKIGSGDFDAQLPPSGNSYEIDHLIHSFAVMQKSLKEYIKNLEITNAEKNRIL
                     AEVQVASEIQRKLIPANTEHPYKVKEIRCYGILEPAGEIGGDLYDFFPVDESHFFFVI
                     ADVAGKGIVASMTMIMVSTYLRTISTYNFTVNEIMKNLNNFLCTQSSAAIFVTALIGI
                     IDLKNDELEFSNAGHTPLFLRKLNGTYEIFADTHSNPLGVFENMEIGSSQLKLDKGDE
                     LILLTDGVTEIMNEKEDFLGIKGVESIIQNLETTNPEQTAKQIMQYVHNFAQSIPQRD
                     DITILVLDYKYPE"
     gene            complement(2563..3654)
                     /gene="meaB"
                     /locus_tag="PKM80_04140"
     CDS             complement(2563..3654)
                     /gene="meaB"
                     /locus_tag="PKM80_04140"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNV93132.1"
                     /translation="MNEKRKPEWTPLYSGKEFATSVVTGKKSVHIKASPSTKGKKYDL
                     DTLVKGVLNSERTLLAKAITLIESNAEKHFLQGQELIQKLLPYSGNSIRIGITGVPGA
                     GKSTFIESFGLYLTEQGHKVAVLAIDPSSTISKGSILGDKTRMETLSRNPNSFIRPSP
                     SAGILGGVARKTRETIILCEAAGYDIVLIETVGVGQSETTVRSMVDFFLLMQIAGAGD
                     ELQGIKRGILELADLIVFNKADGDNIQRTEIAKQELQNVLHYMRNATEGWQTNVVTCS
                     AINKTGLDEIWHSISEFYTKTKQNGIFAKRRAQQNTQWFENLITEAVLHRFYQKEEVK
                     TLITQLKDDIAIGKIPIALAVSKILKASE"
     gene            complement(3647..>4692)
                     /locus_tag="PKM80_04145"
     CDS             complement(3647..>4692)
                     /locus_tag="PKM80_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNV93133.1"
                     /translation="DFSARIARNTQLYLQNETSICRVIDPWAGSYYVEYLTDALMKSA
                     WNHIMEVENLGGMAKAIETGLPKMRIEEAAARRQAKIDSGADIIVGVNKYPPETEIPI
                     EILEVDNSAVRATQIARLEKLRKERNNEDVEKALEAITKCAETKNGNLLELAIDAARK
                     RASLGEISYAMEKVWGRHQEAIRLINNVYSSEYAKMDDIEQVRALTDKFAELEGRRPR
                     ILVAKMGQDGHDRGAKVVATAYADMGFDVDVGPLFQTPEETARQAVENDVHIIGISSL
                     AGGHKTLLPKLMEELKKYGREDIIVTVGGVIPPQDYEFLYQNGAAAIFGPGTKLPESA
                     KLVMEKLLAHKNE"
BASE COUNT         1421 a          901 c          812 g         1558 t
ORIGIN      
        1 tgcgcaatta atgcagcgaa cctatctgat gttggtaggt aaaaaaccag aggaataaac
       61 tcgatagctg attttaatgg aaagagaata ggtttgtgga aaactgatga ccgcctcctc
      121 tcattaatct ttctacagca gcacaagctt aatatggata ttgtagattt ggatacatca
      181 atcaatctct ttctgaaaga tgggatcgat ataatgaatg tgatgcgtta caatgaatat
      241 catcaacttc agcaagcagg aatagatatt gaggatttat ttacagtatc atttgcagat
      301 attggactta atgttgcaga taatgggctt tatacaacta gggaatttta tgaaaagcat
      361 tcgaaacatt gccgtgattt cgttgacgcc actattgaag ggtggttata tgcaataaac
      421 aatcaagagg aaacaatatc cacagtttta gaaattatgc ataaacacca tttaccagct
      481 aatagacctc atcaaaaatg gatgctaaat gaattaagcc aagattttat ggtggatagt
      541 agtcaaattg gtgttcttca aaaaagtcag tttgaagaag caaagagatt actaactgaa
      601 caaaatatgc ctataaccag ccaaacctat aatagttttt atccgtatgc aaagtaaaca
      661 agaagaacga ggacttgctt accagataat tgtttttatc tttttttttc tggtacttat
      721 tttctctgtc tttataggaa taaccagatt tctgttttcc gaaataatga tggataattc
      781 ccgtgaaact gtaagtcatc ttgctaagga aactgtttat cagattgagg gacgcttatc
      841 agccatagta gatatctctc aatcgattat aacattatat aattctgact atctcaatag
      901 aaacgaactg gatacaattc tgaatgatta tatctatgaa tttgacactt tggaatcaat
      961 tactattgct tatgctccag agtataacaa atctggtgat tcccgaacca tttatcactt
     1021 taagaataag gccatatctc aggaattgca tcctgcagaa tatcaatatc ttgattggtt
     1081 tcaaattccc tatgaactaa aaagtaaatg ttggactgac ccctggtatg atgctagttc
     1141 cacaaagcaa acagttattt cgtattgtgt tcctattttt attaaaggta attgtattgg
     1201 tgttatgcga ttcgatacaa aactttccgg tttgcagaga atcgtttctc ctctaaagct
     1261 aaaacgcagt ggttatgctt ttttagtttc cagcattggt accattatca ctcatcctgc
     1321 agattctata tcttttaatg aaagcatatt tagcttagcg gaagaaacaa aggacaccca
     1381 attacgagaa ctcggcaaaa aaatgattca aggtgaaact gattttattc gtttacgggg
     1441 aaatacaatt tttaacgatt cttggatata tttttctccc ctgctttcca ataactggtc
     1501 actgggaatt gttattgcgc ataaagatgt cgttagagac ctaaatttgt tgctgattat
     1561 tcaaaccctc ttttcggtga ttatatttat atctatatcg ctaattgtat attacagaac
     1621 tttcagtgtc tctaaaccca ttagactttt tacagaagta gccactaaaa taggttcagg
     1681 tgattttgat gctcagcttc ctccttcagg aaacagctat gaaatagatc accttataca
     1741 ttcttttgct gtaatgcaaa aatctttgaa agagtatatc aaaaatttgg aaattacaaa
     1801 tgctgaaaaa aatcgcattc tggctgaagt acaagttgct tctgaaattc aacgaaaact
     1861 aattcctgca aatactgaac atccctacaa agtaaaggaa atccgctgtt atggtatttt
     1921 ggaaccagct ggtgaaatcg gaggtgattt atacgatttc tttcctgttg atgaatctca
     1981 tttctttttt gttatagctg atgtagctgg taaaggcatt gtggcttcaa tgacgatgat
     2041 aatggtttct acttatctaa gaactatttc cacttataac ttcactgtaa atgaaataat
     2101 gaaaaacctc aataattttc tgtgtactca atcttccgcg gcgattttcg ttactgctct
     2161 aataggaata atagatttaa aaaatgatga actggaattt tccaatgctg gtcatactcc
     2221 cttgttttta agaaagctga atggtactta tgaaattttc gccgatactc attccaatcc
     2281 tttaggtgtt tttgaaaata tggaaattgg ttcctcccaa ttgaaactgg ataaaggaga
     2341 cgaacttatt ctcctaacgg atggggttac ggaaattatg aatgaaaaag aagattttct
     2401 ggggataaaa ggtgttgaat caataatcca gaacctggaa actaccaatc cggaacaaac
     2461 tgctaagcag attatgcaat atgtgcataa ttttgcacaa agtataccgc aaagggatga
     2521 tataactatt ctggtacttg attataagta ccccgaatga tattattcgc ttgctttcag
     2581 tattttgcta acagctaaag caataggaat cttaccgata gcaatatcgt ctttcaactg
     2641 agtgatgagt gttttcactt cctctttctg gtagaaacga tgcaaaactg cttctgtgat
     2701 caaattttca aaccactgtg tattttgctg tgcacggcgt ttagcgaaaa tcccattttg
     2761 ttttgtcttt gtgtaaaatt ccgaaatcga atgccaaatc tcgtctaaac cggttttgtt
     2821 tattgccgaa caggttacaa cattagtttg ccaaccctcg gttgcatttc gcatataatg
     2881 taaaacattt tgtagctctt gtttagcgat ttccgttcgt tggatattat ctccatcggc
     2941 tttattaaac acaatcaaat cagcaagttc taaaattcct cttttaatac cctgaagctc
     3001 atctccagca ccggcaattt gcatcagcaa aaagaaatct accatagaac gaacagtagt
     3061 ttcggattga ccaactccaa cagtttcaat taatacaata tcataacctg ctgcttcgca
     3121 taatattatt gtttctctgg tttttctggc tactccaccc aaaattcccg cactgggaga
     3181 aggacgaata aagctattgg gatttctgga cagggtttcc atccttgttt tatcgcccaa
     3241 tatacttcct ttactaatag tagagctagg atcgatagct aaaacagcta ctttgtgccc
     3301 ttgttcagtt aaatataagc caaaactctc tataaaagtg cttttccctg ctcctggaac
     3361 tcctgttata ccaatacgaa tagaatttcc agaataaggc aggagctttt gaattagctc
     3421 ctgcccttgt aaaaagtgtt tttctgcatt actttctatc aaagtaattg ctttggccag
     3481 caaggttcgc tcggaattta gaactccctt taccaaagta tctaaatcgt attttttccc
     3541 tttagtagaa ggagacgctt ttatatgcac agatttcttt cctgttacaa cactcgtggc
     3601 aaattccttt ccactatata aaggagtcca ttccggtttt cttttttcat tcattcttgt
     3661 gtgccaatag cttttccata actaattttg cagattcagg aagtttggtt ccaggtccga
     3721 atatagcagc agctccattt tgataaagga actcataatc ctgaggagga attacacctc
     3781 caactgtaac aattatatct tctctgccat atttcttaag ctcttccatc agtttaggaa
     3841 gtagggtctt atggccacca gccaaagaac ttatgccaat aatatgaaca tcattttcca
     3901 ctgcctgacg agctgtttct tcaggagttt gaaatagagg tcctacatca acatcaaaac
     3961 ccatatcagc ataggcagta gctacaactt tggctccgcg atcatgacca tcttggccca
     4021 ttttggctac tagtatacga ggtcttcgac cttccagttc tgcaaattta tcagttaaag
     4081 cacgaacttg ttcaatatca tccatttttg catactcact gctataaaca ttatttatta
     4141 aacgaatggc ttcctggtga cgcccccata ctttttccat tgcataggaa atttcaccta
     4201 aagaagctct ttttctggct gcatcgatag ccagttctag aagatttcca ttttttgttt
     4261 cagcgcattt ggtaattgct tccagggctt tttcaacatc ttcgttatta cgttctttgc
     4321 gtagtttttc cagacgggca atttgggtgg ctcgcacagc ggaattatcc acttctaata
     4381 tttcgattgg aatttccgtt tccggagggt atttatttac acccacaata atatcagcac
     4441 cagaatcaat ttttgcctgt cgtcgggcag ctgcttcttc gattcgcatt ttaggaagcc
     4501 ctgtttcaat agctttggcc attccaccca agttttcaac ttccattata tgattccaag
     4561 cacttttcat taaagcatcg gttaaatatt ctacataata tgaaccagcc cagggatcaa
     4621 tcactctgca gatgcttgtt tcattttgta aatagagctg tgtgtttctg gcaattctgg
     4681 cagaaaaatc ag
//
LOCUS       DAORHB010000113         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_19721_length_4619_cov_5.887818, whole genome shotgun sequence.
ACCESSION   DAORHB010000113 DAORHB010000000
VERSION     DAORHB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19721_length_4619_cov_5.887818"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKM80_04150"
     CDS             <1..703
                     /locus_tag="PKM80_04150"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HNV93134.1"
                     /translation="LDLSCLPKNNKKKKSSEVIMLTYTEIRSNAREFLRGKWNNPCAL
                     IILILALINFGVSCVPYLGSVISILVTGPFFLGLAIVFLKLVRGEEITIELMFEGFKD
                     FTRSFIAGVLIIVYIFLWSLLLIIPGIIASYAYSMTFFILADNPNLTANEAITASKEL
                     MKGHKTELFLLDLSFIGWILLGILSCGIGFLWIASYIETARAIFYHEIKGEAIVEPIV
                     DATFEEVANKPLEEE"
     gene            complement(711..2093)
                     /locus_tag="PKM80_04155"
     CDS             complement(711..2093)
                     /locus_tag="PKM80_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV93135.1"
                     /translation="MSKIYKKQKDSRLRTSFRTSITEGIFAQIYGNLSVIGSSFIVKL
                     MVILNASPMHYSLLSAIGQVSAIWQPLGVAITHKLRERKNACIFITALGRFLTFFLGL
                     SLLFHSQLQGIWFVLILLFFSAGLQSLGSNIWIAWISDLIPLSIRGRFFSKRNQVLIG
                     VGLIVSYIVSFHIDLFEAAKGAFKASYIHLLKAEHFFIPQNQPWFLAGVFAFASIIGL
                     IGLIFLKRQPENLITSVPELSLRERYLSPFRDKNFRLLLIFGIWWMLATGVGSPFWGP
                     YMMKKLSMSLFEIQLYNTISVCSSLLSYQFWGRFIDNNGNKTAMKICVALGGLNPMLW
                     LFTSPGNYTILWFEALISGCMWAGTGIITTNFVLSVAAKGSEQVYSGVYGAITGLAMM
                     VSTLACGIFFPSALTFGKRVFEPEQVIFGVGGILRWLSFIPLFFVVEKQNVPLHKALI
                     SLFKSPFEKR"
     assembly_gap    2287..2386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2829..4019
                     /locus_tag="PKM80_04160"
     CDS             2829..4019
                     /locus_tag="PKM80_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNV93136.1"
                     /translation="MAQDMNEDRDLYSSELKELLRETLKHSIEGEFSTHSEDPYFYTK
                     KGYPLQTEIDGLPLYPEFIPEVLLTWANLDTTIKRHKEDILVLDLETTGLRSNGIFAF
                     MIGLGYYENEQYIVEQIFLPDPEAELNSFDRLIELIKEKSLLITFNGKTFDVPVLESR
                     LLYHQIWLNLREMEHLDLLHIARRLWKNKLPSCALETLEFYILGQIRDKELDIAGGEI
                     PQTYFNYLINGDPELIRRVFLHNHTDILHTAALFTLICNSISYPPEKGMDIRIDYHSL
                     AMLYQSQNRIDIAKNILLDLLSLGFIDKDILRELGFIYKKEKDYDSAYDCFEIAASLN
                     CPIAMQESCIILEYKYKRYAKALEIAEKLKAYLLSRPIVNLNKVTNTEKRIERLKLKL
                     NKIK"
     gene            4031..>4619
                     /locus_tag="PKM80_04165"
     CDS             4031..>4619
                     /locus_tag="PKM80_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNV93137.1"
                     /translation="MNSVSFGSDNHSGIHPQVLEAIQKANYGYCPAYGDDPLTIKVLE
                     QIKQLFGGNCDAWFVITGTGANILSLQSVIHSYNAIICPKSAHINTDECGAVQKFTQS
                     RLQPIETTDGKLTPELIAPLLLGNRDQHHSQAKVISISQSTEYGTLYSLQELKALADF
                     AHQNNMFLHIDGARLANAASALNCSLKEMTKDISAD"
BASE COUNT         1544 a          813 c          805 g         1357 t
ORIGIN      
        1 acttgactta agttgccttc ccaaaaataa taaaaaaaag aaatcaagtg aggtaattat
       61 gttaacctat actgaaattc gtagtaatgc gcgtgaattt ttacgaggga aatggaataa
      121 tccctgtgca ttgattattc ttattttagc cctgattaat tttggagttt cttgtgtacc
      181 ttatcttggt tctgttattt ctattcttgt tacaggacct ttttttttgg gattagctat
      241 tgtttttctg aaactggtta gaggtgaaga gattacaata gaattgatgt ttgagggctt
      301 taaggatttt acccgtagtt ttattgccgg tgttttaatc atagtttata tctttttatg
      361 gagtttattg ttgataatcc ctggaatcat tgcttcgtat gcctattcaa tgactttttt
      421 cattctggct gataatccta atttaactgc caatgaagca attacagcta gtaaagaatt
      481 gatgaaaggc cataaaacgg agctcttttt gctggatctt tcttttatcg gttggatttt
      541 actgggaata ttatcttgcg gaattggttt tttgtggata gcaagttaca ttgaaacagc
      601 tagggcaatt ttctatcatg aaattaaagg cgaagcaatt gtggaaccga ttgttgatgc
      661 tacttttgaa gaggtagcca ataaaccgct ggaggaagaa taatttaaat tcagcgcttc
      721 tcaaaaggtg atttaaaaag cgatattaac gctttatgta aaggtacatt ttgcttttcc
      781 actacaaaaa aaagaggaat aaaacttagc caacgtaata ttccccctac cccaaaaatc
      841 acctgttcag gttcaaaaac gcgtttgcca aaagttaaag ccgatggaaa aaatatacca
      901 caggcaaggg tagaaaccat cattgctaaa cctgttattg ctccgtaaac tccagaataa
      961 acttgttcgc tacccttggc tgcaaccgat aaaacaaaat tggtagtgat tattcctgtt
     1021 ccagcccaca tacaacctga aataagtgct tcaaaccata aaattgtata gttgccagga
     1081 ctggtaaata gccaaagcat tggatttaat ccacctaaag caacgcaaat tttcatagct
     1141 gttttgttgc cgttattgtc gataaagcgt ccccaaaatt gatatgataa aagggaagaa
     1201 catacactaa tagtgttata tagctgaatt tcaaataagc tcatagatag ctttttcatc
     1261 atatagggtc cccaaaaagg acttcctact cccgttgcca gcatccacca tatcccaaaa
     1321 atgagtaata aacgaaaatt tttatcccta aaaggtgaaa gataacgttc ccgtaaactt
     1381 agttccggaa ccgaagttat caggttttca ggttgacgct ttagaaagat taacccaatt
     1441 aaaccaataa tagaagcgaa agcaaaaaca ccagctaaaa accagggttg attttgcgga
     1501 ataaaaaaat gctctgcttt taataaatgt atgtagctag ctttaaaagc accttttgcc
     1561 gcttcaaata aatctatatg aaaactaaca atgtagctaa ctataagccc tacaccaatt
     1621 aagacctgat tccgtttgga aaagaaacgc ccgcgaatag atagaggaat tagatcactg
     1681 atccaagcaa tccaaatatt ggatcccaga gattgtagtc cagcactgaa aaaaagcaat
     1741 atcagtacaa accaaatacc ctgtaactga ctatgaaata ataatgatag ccctaaaaaa
     1801 aaggttaaaa aacgccctag ggcagtaatg aatatacagg catttttacg ctccctcaat
     1861 ttatgagtaa tagccactcc caaaggttgc caaattgcag aaacctgacc aattgcactt
     1921 agtaaactgt aatgcattgg cgaagcattt aaaataacca tcaattttac aataaaagaa
     1981 gaacctatca ctgacagatt accataaatc tgagcaaaaa taccttctgt gatagaggtc
     2041 ctaaaactgg ttcgaagacg gctatccttc tgctttttat atatcttgga catttaaatt
     2101 cccttataat ttctattgct cattttatta aggggctgtt cctgtccaga aaattgtaac
     2161 caaaagtgaa ctttcgaaac aaggatttta ggattggttc acgcagatta cgcagatcac
     2221 gcagatttag ttttagaaac aaggatttaa ggatttaagg atatgttcac gcagattacg
     2281 cagattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagga tttaaggatt
     2401 ttaggattaa gaggatttct aatgtaaagt tccgttagga acgatacata ctaacgacgg
     2461 gttttaaccc ggcgcaaaga agaacgaata aaaaaagtcc catcgggacg acagatttga
     2521 tcgatgggtt ttaacccggc gaaaaataca gaaaggtaga taacttcatc aattcacaac
     2581 aaacaaacta tcaagccttc atagaatata gcagaaactt gataactcag ttgcaagctg
     2641 aacgacagga tttcgttctt ccaggatatc aaaagtaaaa aagccccttc ctcctaaaaa
     2701 tcctgatatc ctataatcct cgtttctata atgttggtaa tctctaaaat ctattcgtga
     2761 tacttgacaa aaaccaaagg caatcaaaaa aaggaatata ttagtataga aacttacagg
     2821 ttaggttaat ggctcaagat atgaatgaag accgggattt atactcttcc gaattgaaag
     2881 aacttctcag agaaacatta aaacactcta ttgagggaga attttccact catagtgaag
     2941 acccttattt ctatacaaaa aaaggctatc ccctacaaac cgagattgat ggtttacctc
     3001 tttatccaga atttattccg gaggttttac taacctgggc aaatttagat acaactatta
     3061 aaagacataa agaagatatc ctagttttgg atttggagac tactggttta cgcagtaatg
     3121 gcatttttgc ttttatgatt ggtttgggtt attatgaaaa cgagcagtat atcgtagaac
     3181 agatctttct gcctgatcca gaagcagaat taaattcctt cgatcgcctc attgaattga
     3241 taaaggaaaa atcactgcta attacattca atggtaaaac ctttgatgtg ccggttttag
     3301 aatctcggct tttatatcat caaatatggc ttaatttacg agaaatggaa cacttggatt
     3361 tattacatat tgcccggcga ctatggaaaa ataaacttcc ctcctgtgct ttggaaacct
     3421 tagaattcta tattctgggt cagatcagag ataaggaact tgatattgca ggcggggaaa
     3481 ttccgcagac ctattttaat tacctaatta atggtgatcc agaacttatc cggagagtat
     3541 ttttgcacaa tcatacagat attttacata cagcagcact gttcacttta atttgtaaca
     3601 gcatttccta tcctcctgaa aaagggatgg atataagaat tgattatcat tctttggcta
     3661 tgctgtacca gagccaaaat cgaatagata ttgccaaaaa tatacttctg gatttactat
     3721 cacttggatt tatcgataaa gatattttaa gagaactcgg cttcatctat aaaaaagaaa
     3781 aggattacga ttctgcctat gattgttttg aaatagctgc ttctctaaat tgccctatcg
     3841 ctatgcaaga aagctgtatc attctggaat ataaatataa acgctatgca aaagcgctcg
     3901 aaatagcaga aaaattaaaa gcttatctgc ttagccgtcc aatcgttaat cttaataaag
     3961 tgacaaatac agaaaaacgc atcgaacggc ttaaactaaa gctaaataag ataaaatgag
     4021 gtaaaaataa atgaattcgg taagttttgg ttcggacaat catagtggaa ttcatcccca
     4081 agttttggaa gcaatccaaa aagctaatta tggctactgc cctgcctatg gagatgatcc
     4141 tctaactatc aaggtcttag aacaaatcaa acaacttttt gggggaaatt gtgatgcctg
     4201 gtttgtaatt acaggaaccg gagctaatat actttctttg cagtctgtta tacattccta
     4261 caatgcaatt atctgtccca aaagtgctca tataaatact gatgaatgcg gggcagtgca
     4321 aaaatttacg caaagccgtt tgcaaccaat tgaaactacc gatgggaaat tgaccccgga
     4381 attgattgct cccttacttt taggtaatcg ggatcaacat cattctcagg caaaagttat
     4441 ttctatctcc caaagcacag aatatggaac tttgtattca ttgcaggaac tgaaagcact
     4501 tgccgatttc gctcatcaaa ataatatgtt tttacatatc gatggagcgc gtttggcaaa
     4561 tgctgcttcc gctttaaact gttctctgaa agaaatgaca aaagatattt ctgccgatg
//
LOCUS       DAORHB010000114         4563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_20086_length_4563_cov_4.183230, whole genome shotgun sequence.
ACCESSION   DAORHB010000114 DAORHB010000000
VERSION     DAORHB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20086_length_4563_cov_4.183230"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKM80_04170"
     CDS             complement(<1..406)
                     /locus_tag="PKM80_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV93138.1"
                     /translation="MNILITGSNGFVGSNLMWKLEEEGHQVIGIDISDQCDYKKHSET
                     MIGDIRSLKELNKVSDTYLKKYNSPIDLIIHCAAAKNDFGISHAEYYSHNKYGTKTLI
                     SFANQQNINKMIYFSSVGVFGHPQGEANEDFPY"
     gene            complement(417..1565)
                     /locus_tag="PKM80_04175"
     CDS             complement(417..1565)
                     /locus_tag="PKM80_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV93139.1"
                     /translation="MTIAKIVFWVSWLLLAYHLFGYGLILFIFNALFTKKKSLYPEPD
                     EYPSITVLCPAYNEEKLIEAKIQSFLNLDYPKDKIKMIVISDDSTDKTNEIVEKYTNY
                     NISLVIQKPRAGKQNAHNLVLPMLDTEYILSTDADCLFEPDSVKILVANMLSDRRIGL
                     VSGAVKKVDKGNRESGEGIYWKYESFLKLMESKFKTIIGATGVMYLIKRELFTPISSN
                     SPDDFERVLLIIKNGYYATYEPRASISLEVTEKATEEVQRKIRIITQEWFVLKRNKEL
                     LNPFRYPAVSFIFFSHKLLRWLFFVFVLTGFISNAFLISVGFYRVVFILQIVVYLLGT
                     LGLISQAKGKHIPLTGLAGYFVAMVYSSAVAFFHFLQDKKYDLWNPLR"
     gene            1774..3117
                     /locus_tag="PKM80_04180"
     CDS             1774..3117
                     /locus_tag="PKM80_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNV93140.1"
                     /translation="MSQINLKKNIITSGSFRIVILILSFLISWISARYLGVTLKGEYS
                     YLITLCGFMWTVLDLGVYRSYPYIVRKRPDEVQTLFGWTIITMFFEIVVMTSIGVAFL
                     DFWNKLIGYQLSPVKIIFLVMFVSLNKAFMQLQAIYMGMNRVLENSISHFLISVVFFI
                     LLLIGYFALIDVDRIDILLLFNLIGLACSVLYLAFRNRWGNWYKELNIKTIKLFYSYG
                     FRVFLSSLFIMLLIRADIVLIKHFLDFSEVGIYSLAAHIVDFIQIASNLVGGLLFVKL
                     SDIEDDISKWLLLKKTLLIFFFFITLANLGFGLIGKPVMQIMFGADFVPVYYVYFWLM
                     PAAYGLSFGSLFNNYLNSKGFPIISIILPAIALVLNITLNMLLIPTMGINGAALATSI
                     SYFLWFLLIVYFEQRDSKGKMLHYLMPTWKDVTDLYRETVIEIKEMMNNISHKKA"
     gene            3128..4174
                     /locus_tag="PKM80_04185"
     CDS             3128..4174
                     /locus_tag="PKM80_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV93141.1"
                     /translation="MKDEPVKKVLIIHTWGIGDLILLTPVLQAVKQMYPKLELEVLFF
                     PESAAIPVTNAPFISKIHFTGWKANLLIPTILALRKQKYGAVLFSSGVKPWKTWLFML
                     LLKAEKKIGEYRTIRYPGLSMYIKFNPAFSRTRSNYALFESFLNLPSWEDALSKQNDL
                     HLYPYFHLTEENQKWAEKFLLENKLSGEKVIGIHPGCLAKNKHRRWSKEYFITLINKL
                     QEHYSYPVLIIAGPDEIEVGKAIQARTNTLILSNAALANVAAVISHLHFFINTDSGLG
                     HIASCFGINSLTIFGPANEAQTAPFSANSHIIRYPVSCAPCLEKKKKPKCALDCLVKL
                     TPDMVFKRVQELLI"
     gene            complement(4156..>4563)
                     /locus_tag="PKM80_04190"
     CDS             complement(4156..>4563)
                     /locus_tag="PKM80_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93142.1"
                     /translation="DIQRIDKGVYKFCGYKNHREAISYILGADINLIFLNDDPISTFS
                     FTGKLFELLRAGKPILAMGPKKCIIEDLLKETGAGKYAWIKDKDQIKMKINELLTDTS
                     SFSIQTDIIKQYSRAETCKTLLSIYERGQISNS"
BASE COUNT         1413 a          787 c          775 g         1588 t
ORIGIN      
        1 tataaggaaa atcctcatta gcttctccct gagggtgtcc aaaaacacct actgaactga
       61 aatagatcat tttattgata ttctgctgat tggcaaaact aattagagtt ttggtgccat
      121 atttattatg gctatagtat tccgcgtggg aaattccgaa atcatttttt gcagcagcac
      181 aatgtattat caaatctata gggctgttat actttttaag ataagtatca gagaccttat
      241 tcagctcttt cagagaccgt atatcaccta tcatcgtttc cgaatgtttt ttataatcgc
      301 attggtcact gatatctatt ccgataactt gatgtccctc ttcttctaat ttccacatta
      361 aattggatcc aacaaaaccg ttactgcccg taattaatat attcatttat cctccattaa
      421 cgtaaagggt tccaaagatc atacttttta tcttgcagga aatggaaaaa ggcaaccgca
      481 gaagaatata ccatagctac aaaatatcct gccaaaccgg ttagaggtat gtgttttcct
      541 tttgcttgac ttatgagccc caatgtccca agtagataaa ctactatctg caggatgaaa
      601 acaaccctgt aaaaaccaac tgagatcaaa aaagcgttgg aaatgaatcc tgtcaacaca
      661 aaaacaaaaa acaaccaacg caacagcttg tgcgaaaaga agataaaact gacagctgga
      721 tagcgaaaag gattcaaaag ttctttattc cgctttaaaa caaaccattc ttgggtaata
      781 atcctaattt ttctctgaac ctcttccgtt gctttttctg taacctccag tgaaattgaa
      841 gctcgaggtt cataagttgc ataatatcca ttctttataa tcagtaaaac tctttcgaaa
      901 tcatcggggc tattagaact tataggagta aatagttctc ttttaattaa atacataact
      961 ccagtagcac caataatcgt tttaaatttt gattccatta atttcagaaa tgactcgtat
     1021 ttccaataaa tcccttctcc cgattccctg tttcctttat cgactttttt tacagcaccc
     1081 gataccaaac cgattcttct atcagatagc atattagcta ccagtatttt aacgctatca
     1141 ggttcaaata aacaatcagc atctgtagat aatatatact cagtatcaag cattggcaaa
     1201 actaaattat gcgcattttg tttacctgcc ctcggttttt gaattactag agatatatta
     1261 taattggtgt atttttctac tatttcattg gttttatcgg ttgaatcatc agaaatcact
     1321 atcattttta ttttatcttt gggatagtct aagtttaaaa aagattgaat ctttgcttca
     1381 attaactttt cttcgttata agcaggacat agtaccgtaa tagaaggata ttcatcaggt
     1441 tcgggataaa gagatttctt ttttgtaaac agagcgttga atatgaataa aattaatcca
     1501 tacccgaaaa gatggtaagc taaaagcagc catgaaaccc agaatactat cttcgctatg
     1561 gtcatttatt ttcctttaca tcctatacac caacagttta acaatctgtc aatatttata
     1621 aatcacaatt attaacaggg ttgagctgtc aagcagaatt cactaccact aacagtatcg
     1681 aatttagttt tacaattgac aaaaaggcag ggattatgtt tatggcatta tgaattataa
     1741 tagaaaattt agtgctaatt acccaagaga aaaatgtccc agataaatct gaaaaaaaat
     1801 ataataacaa gcggcagttt cagaatagta atcctgatat tgtcatttct gatcagctgg
     1861 attagtgcca gatatttagg agtaacctta aagggtgaat acagttatct gatcacttta
     1921 tgcggtttta tgtggacagt tcttgattta ggggtttata gaagttatcc ttatatagtg
     1981 cgtaaacgcc cagatgaagt tcagacattg tttggatgga caatcatcac tatgttcttc
     2041 gaaatagtag taatgacctc tatcggagtt gcgtttctgg atttttggaa taaactgatt
     2101 ggctatcagc taagcccagt aaaaataatt tttctggtta tgtttgtttc cttgaacaaa
     2161 gcatttatgc aactacaggc aatatatatg gggatgaata gggtactgga aaattccatc
     2221 tctcattttt taatctcggt tgtattcttt attcttttat taatcggcta ttttgcactt
     2281 atagatgttg atagaattga tatcctgttg ttatttaact tgattggctt ggcttgcagt
     2341 gttttatatc ttgctttccg taaccggtgg ggaaattggt ataaggaatt gaatataaaa
     2401 acaatcaagc tgttctatag ttatggattt cgagtttttc tatcctcatt attcataatg
     2461 ctgttaattc gagccgatat tgtgttaatt aagcactttc tggatttctc agaagtaggt
     2521 atctattctc tggctgctca tattgtagat tttattcaaa ttgcctctaa cctcgtaggg
     2581 ggcttattat ttgtcaagct ttccgatatt gaagatgaca taagtaaatg gcttctgcta
     2641 aagaaaaccc tattgatttt tttctttttt attactttgg cgaatcttgg ttttggatta
     2701 ataggaaaac cagtaatgca gataatgttt ggagctgatt tcgttcctgt ttattatgtt
     2761 tatttttggt tgatgcctgc ggcttatgga cttagttttg gttctctatt taataattat
     2821 ctgaacagta aaggttttcc gataatcagt attatccttc ctgcaatagc tttggtattg
     2881 aatattactt tgaatatgct gttaatacct acaatgggaa ttaatggtgc tgctcttgct
     2941 accagtatat cttattttct ttggtttctt ttgattgtct attttgaaca gcgtgatagt
     3001 aaaggcaaga tgttgcatta tcttatgcct acctggaaag atgtaactga cctctatcga
     3061 gagacagtaa tcgaaattaa agagatgatg aataatatat cccacaagaa agcttaagga
     3121 tagacctatg aaagatgaac cagtaaaaaa agtattaata attcacactt ggggtattgg
     3181 tgatttgata ttattaactc ctgtcctgca ggctgtaaaa caaatgtatc caaaactgga
     3241 actggaagtt cttttctttc ctgaatcagc ggcaattcct gttacaaatg ctccctttat
     3301 aagtaaaata cactttaccg gttggaaagc taacttactt attcccacaa ttttggctct
     3361 gcgcaagcaa aaatatggag cagttctttt ttcttctgga gtaaaacctt ggaaaacatg
     3421 gctatttatg ttacttttga aagccgaaaa aaagataggt gaatatagaa ctatccgcta
     3481 tcctggttta tctatgtata taaagttcaa tcctgcattt tccagaaccc gtagtaatta
     3541 tgcactgttt gagagttttt taaatttacc ttcttgggaa gatgcacttt ccaagcaaaa
     3601 tgacttacat ttatatcctt attttcattt gaccgaagaa aatcagaagt gggcggagaa
     3661 attcttactc gagaacaaac tttcaggaga aaaagttatt ggtattcatc ctggttgttt
     3721 agctaaaaac aaacatagaa gatggagtaa agaatatttt ataactctga taaacaaatt
     3781 gcaagagcat tattcctatc cggttctcat aattgcagga ccggatgaaa ttgaagtagg
     3841 aaaagcaatt caggcaagaa cgaacacctt gattttgagc aatgcagctt tggcaaatgt
     3901 ggcagctgtt atttcgcact tacatttctt tattaataca gattctggct taggacatat
     3961 tgcctcctgc tttgggataa atagtttaac gatttttgga cctgccaatg aagctcaaac
     4021 agctcctttt tctgctaata gccatattat tcgttatcct gtctcctgtg ctccttgcct
     4081 agaaaagaaa aagaaaccta agtgtgcatt agattgcttg gttaagctta ctcctgatat
     4141 ggtttttaag cgcgttcaag aattactgat ttgacctctt tcatatatgg ataaaagtgt
     4201 cttgcaggtt tctgcgcgag aatattgttt aataatatcg gtttgaatag aaaacgaaga
     4261 tgtatctgtt agcaattcat taatcttcat tttaatttgg tctttatcct ttatccaggc
     4321 atatttgcct gcgcctgttt cttttagtaa gtcctctata atacattttt tgggtcccat
     4381 cgctaaaatt ggctttccag ctctcagtag ttcaaatagc ttccctgtaa aactaaaagt
     4441 tgaaattgga tcatcgttta gaaaaatcag atttatatca gctcccagaa tgtaagatat
     4501 agcttcccgg tgatttttat aaccacagaa tttataaact cccttatcta ttctttggat
     4561 atc
//
LOCUS       DAORHB010000115         4524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_20330_length_4524_cov_4.550906, whole genome shotgun sequence.
ACCESSION   DAORHB010000115 DAORHB010000000
VERSION     DAORHB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20330_length_4524_cov_4.550906"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /gene="rplO"
                     /locus_tag="PKM80_04195"
     CDS             complement(<1..435)
                     /gene="rplO"
                     /locus_tag="PKM80_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNV93143.1"
                     /translation="MLTLSDLQRSAGRKDKKRLGKGQGSGWGHQAGRGHKGKKARAGG
                     NVPPYFEGGQMPMNRRLPKRGFKNIFRENYRTLNLYRLQYIDITDLDIPTMEKMGLIP
                     TKGKKANAPVKVLAGVKDEFTKTVNIKANAFSQKAKEIIEKNG"
     gene            complement(438..623)
                     /gene="rpmD"
                     /locus_tag="PKM80_04200"
     CDS             complement(438..623)
                     /gene="rpmD"
                     /locus_tag="PKM80_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNV93144.1"
                     /translation="MKLKVTQIRSTINRIEDHKRVIKALGLGRIGKSRIHNDSPTIRG
                     MINKVGYLLKVEELEEE"
     gene            complement(620..1138)
                     /gene="rpsE"
                     /locus_tag="PKM80_04205"
     CDS             complement(620..1138)
                     /gene="rpsE"
                     /locus_tag="PKM80_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNV93145.1"
                     /translation="MNYEQNHMEEEKLIEKIIETKRVAKVVKGGRNFSFSAIVVVGDK
                     QGNIGVGNGKANEIVDAIRKAKERAVKNMFKVPIVRGTVPHEIIARYGASRVMIKPAA
                     PGTGIIAGGTTRAIFEAAGIENILCKTLGSNTPTNVVKATINGLKSMRTLSDISRLRN
                     KTMAQLTGQEEK"
     gene            complement(1148..1519)
                     /gene="rplR"
                     /locus_tag="PKM80_04210"
     CDS             complement(1148..1519)
                     /gene="rplR"
                     /locus_tag="PKM80_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNV93146.1"
                     /translation="MITPSCKIKNDLRKRRHFSIRKRVSGTSERPRLVVFRSLKHIYA
                     QVIDDTKGETLISMSTIAKDMGTLTGKKKTEQSYEVGVKLGEKALAAGIKKVAFDRSG
                     YKYHGRIKALAEGARKAGLDF"
     gene            complement(1537..2109)
                     /gene="rplF"
                     /locus_tag="PKM80_04215"
     CDS             complement(1537..2109)
                     /gene="rplF"
                     /locus_tag="PKM80_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNV93147.1"
                     /translation="MSRIGKAPIKLNSETKVNVTNNVITVKGKLGELKWTLLPGITIE
                     IEDSVLRVNRADDSKDQRALHGLTRALLQNMVTGVTEGYQKTLHIYGTGYSSEVVGPW
                     LRLSLGFSHDILLQIPKELQVSAEAVPRSKGTRSDFQSIITIKGIDKQLVGQFAAEVR
                     NCRPPENYKGKGIRYIDEHVIIKAGKAGTK"
     gene            complement(2121..2534)
                     /gene="rpsH"
                     /locus_tag="PKM80_04220"
     CDS             complement(2121..2534)
                     /gene="rpsH"
                     /locus_tag="PKM80_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNV93148.1"
                     /translation="MSISDPIADALTKIRNAYRAGHSQVIVNHNKLVEALVNILAEEN
                     FVNSVQVLDRDPENHINYKRILVILRYTNDGEPVLQGIQRISKPGRRVYVKADNLPCV
                     YNHTGCAIISTSEGVMVDRDARLKRVGGEYVCKVW"
     gene            complement(2552..2737)
                     /locus_tag="PKM80_04225"
     CDS             complement(2552..2737)
                     /locus_tag="PKM80_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNV93149.1"
                     /translation="MAKKSLIIKQQRTPKFKVRKYNRCKICGRPRAYMRDFGMCRLCF
                     RKYASEGQIPGITRSSW"
     gene            complement(2747..3292)
                     /gene="rplE"
                     /locus_tag="PKM80_04230"
     CDS             complement(2747..3292)
                     /gene="rplE"
                     /locus_tag="PKM80_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNV93150.1"
                     /translation="MNRLKERYKTQVVEALTKRFGYHNPHQVPKMEKIVVSMGVGQAT
                     QNKALLDNAVKDMELICGRKPVVTKARKSISNFKLRKGMPIGCKVTLRNEVMYEFYDR
                     LISLAIPRIRDFRGIPVDAFDGRGNFSFGLKEQTVFPEIDYDKIDTTRGLNITIVTTA
                     KTDEECRALLQEMGMPFQKSE"
     gene            complement(3306..3611)
                     /gene="rplX"
                     /locus_tag="PKM80_04235"
     CDS             complement(3306..3611)
                     /gene="rplX"
                     /locus_tag="PKM80_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNV93151.1"
                     /translation="MHFKKGDLVKVISGDDRGKKGHILKVFPKTGKVIVEKVHLIKKH
                     SKPTQQNPQGGIITMEAPINASNVMLFNEKLNAVSKPVYQIREGRRIRVCKKTGDEI"
     gene            complement(3635..4003)
                     /gene="rplN"
                     /locus_tag="PKM80_04240"
     CDS             complement(3635..4003)
                     /gene="rplN"
                     /locus_tag="PKM80_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNV93152.1"
                     /translation="MIQAQTMLNIADNSGAKKAMCIKVLGGTKRKYASIGDVIVVAIK
                     SATPGGRVKKGSVEKAVIVRTRKEIRRPDGSYIRFADNAAVIIDEKYEPKGTRIFGPV
                     ARELREAHYMKIVSLAPEVL"
     gene            complement(4019..4255)
                     /gene="rpsQ"
                     /locus_tag="PKM80_04245"
     CDS             complement(4019..4255)
                     /gene="rpsQ"
                     /locus_tag="PKM80_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNV93153.1"
                     /translation="MIKQGIVVSDKNDKTIVVRVERQFIHPLYKKTVRRHKKFMAHDA
                     NNAAHIGDVVQIVESRPLSARKRWALHKIVERSK"
     gene            complement(4282..4476)
                     /gene="rpmC"
                     /locus_tag="PKM80_04250"
     CDS             complement(4282..4476)
                     /gene="rpmC"
                     /locus_tag="PKM80_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNV93154.1"
                     /translation="MKADELRDLSIDELQVKLEELRIELFNLRFQKAKNLLDRPDRIR
                     NVRHSIARIYTIMKEKEKKD"
BASE COUNT         1168 a         1023 c          774 g         1559 t
ORIGIN      
        1 accgttcttt tcaataatct ctttggcttt ttgtgaaaaa gcattagctt tgatattaac
       61 ggttttggtg aattcatctt tcacaccggc taaaactttc accggcgcat tggctttttt
      121 ccctttggtg ggaattaatc ccatcttttc catagttgga atatccagat ctgtaatatc
      181 gatatactgt aaacgataca gattcaaagt gcggtaattt tcccggaaga tatttttaaa
      241 accgcgtttg ggaagacgac gattcatagg catctgtcca ccttcaaaat aaggaggtac
      301 atttccacct gcacgggctt ttttcccttt gtgtccacga cctgcttggt gtccccaacc
      361 agaaccttgt ccttttccta ggcgtttttt atcttttctt cctgctgatc tttgtaaatc
      421 ggataatgtt aacatcgtta ttcctcctcc agttcttcaa cttttaagag gtaaccgacc
      481 ttgttgatca tacctcggat agtggggcta tcattatgaa ttctgctttt ccctattctc
      541 ccaagaccga gagcttttat aaccctttta tggtcttcga tgcgatttat tgtactgcgg
      601 atttgagtaa ctttcagttt catttctcct cctgaccagt aagttgagcc attgtcttat
      661 ttcttaagcg agaaatatcg gacaatgtac gcatcgattt taagccatta attgtagctt
      721 taacaacatt agtgggggta ttagagccaa gtgttttaca caaaatattt tcaattcccg
      781 cggcctcaaa aatggctcga gtagttccac ccgcaataat tccagttcca ggagcagcag
      841 gttttatcat tacacgagat gctccgtagc gagctatgat ttcgtgagga acagtccctc
      901 tcacaatagg cactttgaac atatttttta cggctctttc tttcgctttg cgaattgcat
      961 ccacgatttc gttcgcttta ccatttccta caccaatgtt gccttgttta tcaccaacaa
     1021 ctacaatagc ggaaaagctg aagtttctgc cacctttcac aactttggcc acacgcttgg
     1081 tttcgattat cttttcaatt aatttttctt cttccatatg attttgttcg taattcatct
     1141 ctgctcctta gaaatccaat ccggctttac gagcaccttc tgcaagggct tttattcttc
     1201 catgatattt ataaccggag cgatcaaaag ctactttttt aattccggca gcaagtgctt
     1261 tttcaccaag ttttactccc acttcgtagc tttgttcagt ttttttcttc ccagttaaag
     1321 tacccatatc tttggctata gtagacatcg agatcaaagt ttcacctttc gtatcatcta
     1381 taacctgtgc atatatatgc ttcagagaac ggaaaactac taagcgagga cgttctgatg
     1441 ttccgctaac tcttttccgg atagagaaat gacggcgttt ccgaaggtca tttttaatct
     1501 tacagcttgg agttatcata atcttttcac ctttccttat ttagtcccgg cttttccagc
     1561 ttttataata acatgttcat caatatagcg tatgcctttg cctttataat tttctggagg
     1621 acggcaattt cgtacttctg cagcaaactg accaaccagc tgtttgtcta tacctttaat
     1681 agttattata ctttgaaaat ctgatcgagt acccttggag cgtgggacgg cttctgcact
     1741 cacttgtaac tcttttggaa tctgcaatag gatatcatgt gaaaaaccaa gggaaagtct
     1801 tagccaaggt cctactactt ccgaagaata accagttcca tatatatgca gagttttttg
     1861 atacccttcc gttacaccag taaccatatt ttgtaaaagg gcacgagtta aaccatgtaa
     1921 agcacgttga tctttactat catcagcacg atttactctt aaaacactat cttcaatttc
     1981 aatagtaatt ccgggaagaa gtgtccactt cagttctccc aatttacctt taacagtaat
     2041 cacattatta gttacattca cttttgtttc agagtttaat ttaataggag cttttccaat
     2101 gcgtgacatc tttttccccc tcaccaaacc ttgcaaacat attcgccacc aacacgtttc
     2161 aggcgcgcat cgcgatctac catcacacct tcgctggttg aaataatagc acaaccagta
     2221 tgattataga cgcagggcaa attatcggct tttacataaa cccgtcttcc gggtttggaa
     2281 attctctgaa ttccttgcaa cactggctcc ccgtcattag tatagcgtag aattactaaa
     2341 atacgcttgt aattaatgtg attttcgggg tctctatcaa gaacttggac actattaaca
     2401 aagttttctt cggcaaggat attaaccagt gcttccacga gtttattatg gttaacaatc
     2461 acctgcgaat gtccggcacg atatgcatta cgaatttttg tcaatgcatc agctatcgga
     2521 tcactaatac tcatttatat cctccatttt attaccagct acttctggtt attccaggaa
     2581 tctggccttc cgaagcatat ttgcggaaac ataaacggca cattccaaaa tcgcgcatat
     2641 aggcgcgtgg gcgtccgcaa atcttgcaac ggttatattt tctaactttg aatttaggag
     2701 ttcgttgttg ttttatgatg agtgattttt tggccactaa tcctccttat tcgctttttt
     2761 gaaaaggcat acccatctct tgtaacagag ctcggcattc ctcatctgtt tttgctgtgg
     2821 ttactatcgt gatatttaat ccacgggtag tatctatctt atcataatca atttctggaa
     2881 acacagtttg ttcctttaag ccaaaggaga aatttcctct gccatcaaag gcatctaccg
     2941 gtatacctct aaaatctcgt attctgggta ttgctaaaga tatgagacga tcgtaaaatt
     3001 catacataac ttcgtttcta agggttactt tgcaaccaat tggcatccct ttacgcagct
     3061 taaaattaga gatagattta cgagctttag ttacaacagg tttacgccca caaataagtt
     3121 ccatatcctt tacagcatta tctaaaagag ctttattctg agttgcttgc cctaccccca
     3181 tactaactac aatcttttcc attttgggaa cctgatgggg attgtggtaa ccaaatctct
     3241 ttgttaaagc ttctaccacc tgagttttat agcgttcttt caaacggttc atttattcat
     3301 ctcctctata tctcatcacc tgtcttttta cagacacgga tgcggcggcc ttcgcgaatc
     3361 tgatatacag gtttagaaac agcattcaac ttctcgttga aaagcatcac attggaagcg
     3421 tttataggtg cctccatcgt tattatgcct ccctgaggat tttgctgagt gggtttagag
     3481 tgttttttta tcaaatgaac tttctctaca atgacctttc cggttttagg aaagacctta
     3541 agaatatggc cttttttacc tctatcgtca ccggagatta ctttcacgag atcgcctttt
     3601 ttaaaatgca atttattctt tgccacattt cctcctaaag aacttccgga gccagggata
     3661 ctatcttcat ataatgcgct tcacgaagtt cacgagcaac cggtccaaat atacgagtcc
     3721 ctttaggttc atatttttca tctataataa ctgctgcatt atcagcaaaa cgaatatagg
     3781 agccatctgg acgccgtatc tctttccggg tgcgaacgat tacagctttt tccacacttc
     3841 ccttttttac tctgccgcca ggagtggcac ttttaatcgc tactactatt acatcaccga
     3901 tcgatgcata tttacgcttc gttccaccta aaaccttgat gcacatagct ttcttggcac
     3961 cggagttatc agcaatattg agcattgttt gtgcttgtat cattttaaaa tatcctcttt
     4021 atttacttct ttccacgatt ttatgaagag cccaacgttt tcttgcgctc agaggacggg
     4081 attccacaat ctgaactaca tcacctatat gagctgcatt atttgcatcg tgagccataa
     4141 atttcttatg gcgccggacg gtctttttat acagtggatg aatgaattga cgctctacac
     4201 gcactacaat ggttttatcg tttttatcgc taacgacaat tccttgttta atcattttat
     4261 gtgttgttgc cacttagcac ctcagtcctt tttctctttt tctttcataa tagtgtaaat
     4321 cctggctata ctatgacgga catttctaat acggtcagga cggtcgagca gattttttgc
     4381 cttttgaaag cgaaggttaa atagctcgat acgaagttct tccagcttta cctgtaattc
     4441 atcaatactt aagtcacgca gttcgtctgc tttcataatt ccactccttc gcgtgtgata
     4501 aagcgagttt tgatcggcag ttta
//
LOCUS       DAORHB010000116         4507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_20446_length_4507_cov_5.082884, whole genome shotgun sequence.
ACCESSION   DAORHB010000116 DAORHB010000000
VERSION     DAORHB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20446_length_4507_cov_5.082884"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1042
                     /locus_tag="PKM80_04255"
     CDS             233..1042
                     /locus_tag="PKM80_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93155.1"
                     /translation="MLTIDWKERLNMDTADFLKNKLPKGDYDFEIIFIAYPERVNGKI
                     PAEVISQVANNIVQQLGKKHELYLPFYNALWAKKGDYGKQAFAQILSKLLHRKPSVYM
                     PVLEGAISTADEAEINTLLDKVMLPLIRKYPDKYLDKVFEFGNSQNLDLQSAALNLLI
                     KLIKKREDLIPQVVNYFIHQWLYPLGEAIAVHIAMLKTVAKIDPEYYVNIWREYGSSR
                     DPQIVELLCASITDYYPELENSIEIWTKSGNARLKKAATSAQRILMKKKRS"
     gene            1050..1529
                     /gene="greA"
                     /locus_tag="PKM80_04260"
     CDS             1050..1529
                     /gene="greA"
                     /locus_tag="PKM80_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNV93156.1"
                     /translation="MDLSQYITKTGMQRLQKRINELLTERPEVIKAIAIAREFGDLSE
                     NAEYKAARERQRAIDNEIDYLRCRSAKLQVIDTSEFPKDIVRFGSYCVAEDISNNECI
                     CYHLVGADELNYYDEEGIQEVSVVSPIGRALLSKKIGEIAVVHAPMGERHLKIIEIK"
     gene            1550..2962
                     /locus_tag="PKM80_04265"
     CDS             1550..2962
                     /locus_tag="PKM80_04265"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNV93157.1"
                     /translation="MKNTKPEKEALAYQRKNFWKEAPLEEQKKAMEYAEPYINFLNQA
                     KTERETVAYTVDLLLKNKFTPLSKKGSKKVYSIFRNKTMAVAVIGTDPVSNGFNMVAA
                     HVDSPRVDLKQNPLYEDSNSAMGCMRTHYYGGIKKYQWVSTPLALHGVIIKNDGSTLN
                     ICLGEKEDEPVFIIPDLLPHLAHKEQYTKNLGDAIDASKMNLVFSGMQEPNSDEKEAI
                     KAYALKLLNEKYGITEQDFLSAELQLVPAHKARNCGFDNSMIVGYGQDDRISAYNALM
                     ALINNIPEKPKRTMVVYFSDKEEVGSQGSTGAHSIFIRDFISDLLAHNQEDNGSANLR
                     KTFMNSQILSADVSAAIDPNYPNVHERQNAILFNYGIGINKFTGSGGKYGCNDANAEF
                     LAKVLRIFNEAGIYWQLGELGKVDEGGGGTIAYILSNLGAEVLDCGVGLMGMHSLYEL
                     SSKADLYSTYLAYKAFLKAE"
     gene            2966..3541
                     /locus_tag="PKM80_04270"
     CDS             2966..3541
                     /locus_tag="PKM80_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93158.1"
                     /translation="MPKYLLPLCFFVLCANFLLSQESLALDSIATSIAEEIQIGQPIF
                     LDINCGDWNPVLTEKLSSKLLEAGADLRYNLSDGKGAITFTEEGKAIDLSAFGLSEAL
                     LIQVNLNILWKEESKSSFFSYKTQRYPVHIFETRQIELPSQQLKKISTYSLPLPENKT
                     SGTSSLHRHWFEPIVAGAAIGSLVFLLWNFD"
     gene            3558..4292
                     /gene="bamD"
                     /locus_tag="PKM80_04275"
     CDS             3558..4292
                     /gene="bamD"
                     /locus_tag="PKM80_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNV93159.1"
                     /translation="MRKYLSLIILIVICLFSCSSNKTQLTTEAKLKNADELYAKGKYS
                     RAAVIYEEISFERKSAATAYATMKVADCYYNMHKFSEARLRYEQFISSFPDHEKVADA
                     YFRIGECFFEESLPPHYDQTETIKCIEAFKTFIDRYPSDPRYGEAIDYIQKCQYKLLE
                     KDYLTGYLYYKMKDYSSALMYFDEIIELGNINDLELKSLYYSAKLHLHQKNYEKAKAN
                     YDKLLSRYPNSKEAEKLKRKFEKSVL"
     gene            4294..>4507
                     /locus_tag="PKM80_04280"
     CDS             4294..>4507
                     /locus_tag="PKM80_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNV93160.1"
                     /translation="MQGVVAVLGGTFDPIHLGHLQIATEIIKQKAADSVLFVPSGHHH
                     FKKNTIILPYAKRYSLVKKAIRNNPKF"
BASE COUNT         1493 a          783 c          888 g         1343 t
ORIGIN      
        1 gacagcggac tctggatccg tatgtgaggg ttcaactcct tccatcccag ccattgtttt
       61 taatttttaa attttgggtt gcggtagaat aatttataag cgggcacaat atagtatgtg
      121 ctttgctaat gccctcgtta ataagacggt tgtatttttc tattgacaag attgggcaat
      181 tttttgattt attaccaaat agagaaaata taagtgatat tgggagagat tgatgcttac
      241 cattgactgg aaggaacggc tgaatatgga tactgcagat tttctgaaaa ataagcttcc
      301 gaaaggtgat tatgattttg aaattatctt tattgcatat ccggaaaggg taaatggcaa
      361 aattccagcg gaggtcatct ctcaagttgc aaataacata gttcagcaat tagggaaaaa
      421 acatgagttg tatcttcctt tctataatgc attgtgggcg aagaagggag attatggcaa
      481 gcaggcattt gctcagattc ttagcaagct tttacatcga aagccttcag tgtatatgcc
      541 tgttttggaa ggagcgattt caacagctga cgaagctgag ataaatactc ttttggacaa
      601 ggttatgtta cctcttattc gtaaatatcc tgataaatat ctggataagg tttttgaatt
      661 tgggaacagc caaaatttgg atttacagag cgctgcttta aatttgttaa taaagctaat
      721 taaaaagcgg gaagatctta taccccaggt tgttaattac tttatacacc agtggcttta
      781 tcctttggga gaagctattg ctgttcatat agcaatgctg aaaactgttg ctaagatcga
      841 tcctgaatat tatgtaaata tttggcggga atatggcagc agccgtgatc ctcaaattgt
      901 agaattgctt tgtgcttcta taacggatta ttatccggag ctggaaaatt cgatcgaaat
      961 ttggacaaag agtggaaatg cccgtttaaa gaaagcagct acttcagcac aacgcatttt
     1021 aatgaagaaa aaaaggagct aattaaaact tggatttaag ccaatatatt accaaaactg
     1081 gaatgcagcg actgcagaaa cgaataaatg aattacttac cgagcgtccg gaagttatta
     1141 aagcaattgc tattgctcgc gaatttggtg atctcagtga aaatgcagaa tataaagcag
     1201 ccagagaacg ccaacgagcc atagataacg aaattgatta tctcagatgc cgcagtgcca
     1261 aattgcaagt aattgatacc agtgaatttc ctaaagatat cgttcgtttt ggatcttatt
     1321 gtgttgctga agatattagc aacaatgaat gtatttgtta tcatctggta ggagctgatg
     1381 aattaaatta ttacgatgaa gagggcatac aagaagtttc tgtagtttcc ccgataggca
     1441 gagccctttt aagtaaaaag atcggagaaa ttgcagttgt tcacgctccc atgggtgaaa
     1501 gacatttaaa aattatagaa attaaataaa taaaacaatt ggagaagcaa tgaaaaacac
     1561 taaaccagaa aaagaagctc tcgcctacca gcgcaagaac ttttggaaag aagctcccct
     1621 cgaagaacaa aagaaagcta tggaatatgc agagccatac ataaactttc tcaatcaggc
     1681 aaaaacagag cgcgaaactg ttgcctacac tgtagattta ctgcttaaga ataaatttac
     1741 tcctttaagc aaaaaaggta gcaaaaaagt ttatagcatc tttcgtaaca aaacgatggc
     1801 tgtagccgtt attggaactg atcctgtttc taatggattc aatatggtag ctgctcatgt
     1861 agattcaccc agagttgatc ttaaacaaaa tcctctatat gaagacagca acagtgcaat
     1921 gggttgtatg cgcactcatt attatggtgg tataaaaaaa tatcagtggg tatctactcc
     1981 cttggcttta cacggagtta tcattaaaaa tgatggttcc acattgaaca tttgccttgg
     2041 tgaaaaggaa gacgagccag tttttataat ccccgatctt ttaccgcatt tagctcataa
     2101 agaacaatat actaagaact taggggatgc tattgatgct agcaaaatga acctggtttt
     2161 ttccggaatg caggaaccta attcagatga aaaagaagca attaaagctt atgccttaaa
     2221 gctgcttaac gaaaaatacg gcatcaccga acaagatttt ttaagcgctg aattgcaatt
     2281 agtgccagct cataaagctc gtaattgtgg ttttgataat tctatgattg ttgggtatgg
     2341 acaggatgac cgcattagtg cctataatgc tttaatggct ttaataaaca acattcctga
     2401 aaaaccaaaa cgcacaatgg tagtttattt ctccgataaa gaagaagttg gcagtcaggg
     2461 tagtactggt gctcacagta tttttattcg cgattttata tccgatctgc ttgctcataa
     2521 ccaagaagat aatggtagtg ccaatttgcg taaaaccttt atgaacagcc agatattaag
     2581 tgctgatgtg tctgcagcaa ttgacccaaa ttatcccaat gtgcatgaaa gacagaatgc
     2641 cattcttttc aattatggaa ttggaattaa caaatttacg ggtagcggtg gaaaatatgg
     2701 ttgcaacgac gctaatgccg aatttttagc caaagttctc agaatattta atgaagctgg
     2761 aatttattgg caattaggag aattgggtaa agttgatgaa ggtggcggtg gcacaattgc
     2821 ttacatactt tccaacttgg gagccgaagt tttagattgt ggagttggtt taatgggtat
     2881 gcattccctt tatgaactta gttctaaagc cgatttatac tccacttatc tggcttataa
     2941 agcattttta aaagctgaat aagctatgcc aaaatatctg ctgcccctct gtttttttgt
     3001 gctatgtgct aatttcttgt tatctcaaga gtccttagca ttggatagta tagcaacaag
     3061 catagctgaa gaaattcaga tagggcagcc gatttttttg gacattaatt gtggggactg
     3121 gaaccccgtt ttaactgaaa aattaagttc caaactttta gaagcaggag cagacctgcg
     3181 ttataatcta tccgatggta aaggcgccat tacttttacc gaagagggaa aagctataga
     3241 cctttcagct tttggactaa gtgaagcatt actaattcaa gtgaatctga atatcctctg
     3301 gaaagaagaa agcaaaagca gttttttttc ctataaaaca cagcgttatc ctgttcacat
     3361 ttttgaaacc aggcaaattg aactaccttc gcaacagctg aaaaaaatat ctacttacag
     3421 tttacctctt ccggaaaaca aaacatccgg aacttcatct ttgcataggc attggtttga
     3481 gcccatagtt gcaggagcag ctattggttc attagttttt ctcctttgga attttgatta
     3541 aaaaaaagga tactttcttg cgtaaatatc tatcattaat tatacttatt gttatctgcc
     3601 tgttttcctg cagcagcaat aaaacccagc ttactaccga agcaaaatta aaaaatgccg
     3661 atgagcttta tgccaaagga aaatattccc gagcagcagt tatctatgaa gaaatttctt
     3721 ttgagcgtaa atctgcagca accgcatatg ccactatgaa agttgccgat tgctattata
     3781 atatgcataa atttagcgaa gcacgcctca ggtatgaaca atttatcagt tcttttccag
     3841 accacgaaaa ggttgctgat gcttatttcc gcattggaga atgctttttt gaagaatcat
     3901 tacctccgca ttacgatcaa accgaaacaa ttaaatgcat cgaagcattc aaaaccttta
     3961 tagatcgcta tccgagtgat ccgcgctatg gtgaagctat tgattatatc caaaaatgcc
     4021 agtataagct tttggaaaag gattatctaa ccgggtacct atattacaaa atgaaggact
     4081 atagcagcgc cctaatgtat tttgatgaaa tcattgaatt gggtaatatt aacgacttag
     4141 agctaaaatc gctatattat tctgccaaat tgcatctgca tcaaaaaaac tatgaaaaag
     4201 ccaaggctaa ctacgacaaa ttattaagcc gttatcctaa ttccaaagag gcagaaaaac
     4261 taaaaagaaa attcgaaaaa tcggtattat aaagtgcagg gtgtagttgc tgttttaggt
     4321 ggaacttttg accctatcca tttgggacat ttgcagatag caaccgaaat cataaagcaa
     4381 aaagctgccg atagcgttct ttttgtcccc agtggacatc accattttaa aaaaaatacc
     4441 atcattctcc cctatgcaaa acgctattcc cttgtgaaaa aagctattag aaataatcct
     4501 aaatttg
//
LOCUS       DAORHB010000117         4472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_20683_length_4472_cov_8.082182, whole genome shotgun sequence.
ACCESSION   DAORHB010000117 DAORHB010000000
VERSION     DAORHB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20683_length_4472_cov_8.082182"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2252)
                     /locus_tag="PKM80_04285"
     CDS             complement(93..2252)
                     /locus_tag="PKM80_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNV93161.1"
                     /translation="MGSVVLAIPDSYATVLFSDSVCLGLMLLTITLLSPVVGLSGLFS
                     LIVAILAVRILGFEHWESKSGITAFNSLLTGMMVGYYYPMQIISSAPINYLCFLLLVS
                     LLTLLLYLFSSYLFHQLFRLPSMSLSFSIVAILLWYYFARKGYLSNYPFDKILLFSPA
                     PKLPEFWRLFFISLGSIFFTPEVTAGILVSLALLIITRIGFGLALLGWTLNFILMRLM
                     GVQPGSGVFYSGFNAILIMYAVAGIYLLPSKSSILIGLLATGIGFLFTVLFNVVYYHY
                     NAFTGLYTPLLVPVFAFPFNIAVLLVIFSLRLRLKVSKPIMNDYGVFNPELALQTYQE
                     RYKRFSHLGIPQFAIPLNGLWTITQGNNGMYTHKLDWAYAWDFEMLNSEKRSYDKEDN
                     NLNDYFSYGKPVLASAAGYVVKISDGILDNPIGQINTKENWGNYVCFSHSYGLYSFYA
                     HLKQSSIRIKVGDYVQKGDCIAQLGNSGRSSVPHLHFQVQLGIEPGSPTRLSHLINYK
                     LVKEDKTYEFIGSGIPKEGDTISALVSEPHLQSILALQQHSEQQLQIKCGKKQSTEFW
                     KVNLDLLGNFTIKSTKHTELGFSVYDGIYNSLSLKGNRKSALAAFALLISRLPYLEQQ
                     EIYLTDEPALSVIISPALRHIVLFFSPLFPLFSACAHSKVKTNSEVIEIESDISYRIL
                     GIRYRNRTGKVTIEKYKGLTALELKKKNKTLLQAETF"
     gene            complement(2276..3625)
                     /locus_tag="PKM80_04290"
     CDS             complement(2276..3625)
                     /locus_tag="PKM80_04290"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV93162.1"
                     /translation="MKKHYTAPYIERNSAGNLNKYAHTTVVRHQDNIDGIPIKNLVEK
                     FGTPLFVFSEKHIRHSYRRLLETISIHYPKAEIVWSYKTNYLAAICNIFHQEGAAAEV
                     VSRMEYEKARSNGIPGNRIYFNGPSKRKQDLQLAIQEGAYIHIDHLEELYLIENIADE
                     LAIKPKVAIRVNMDTGISPMWTRFGFNYENGEAYRTVQRLVSGGKIHLAGLHTHIGTF
                     ILDPNAYYWAATKLLNFAQTIKDNFGIVLEYIDMGGGFASSNTLIDQYTPGELASPSL
                     EQYAEAIGTAFNASYYVKEHNPRLILETGRVLIDESGYLIASVLGKKNLPTGERAVIL
                     DAGVNINITAWWYKQRVLPTKPFPGTYQNTVFYGPLCMNIDVLRSAMPFPDLYYGDTV
                     IISPVGAYNVTQWMQFIEYRPNVCLINETGEVDVIRFREDLSDITSRELIPDHLRKI"
     gene            complement(3622..>4472)
                     /locus_tag="PKM80_04295"
     CDS             complement(3622..>4472)
                     /locus_tag="PKM80_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNV93163.1"
                     /translation="GYINSRSKIDVIIPTLDSEIPLYIRLQKELQELGIHTFICTEKQ
                     FNLRDKSKLFHYFTSEDITVPKTVLLNSVAEINKAIQEIEFPVFIKGYLYEAYKANNI
                     IEAQKYFLELQEKWGLPVILQELIEGDEFNVVILGDGKGNCLGMVPQRKLVITDKGKG
                     FGGVVVNNPALEKFAQKVIKTLSWRGPCELEIIKDKEGVFHLLEINPRFPAWVRLAEG
                     CGQNQPAATVLLALGNQIEELPPFKPGVLFIRHSEDIISNINLLGEISVNGELIRKQK
                     KQEEIK"
BASE COUNT         1520 a          866 c          744 g         1342 t
ORIGIN      
        1 tagatgcttt accctgcccc agaagaaatc ctgcaaccaa gataaggaga caggatagaa
       61 taaattgttt cataatataa ctccttataa tattaaaatg tttcggcttg taatagggtc
      121 ttatttttct ttttcagctc taaggctgtt aatcccttat atttttcgat ggttaccttc
      181 cccgttctgt ttcggtatcg gatgcctaaa atacggtaag agatatcgct ctcaatttca
      241 ataacctcgg aattggtctt tactttacta tgtgcacaag ctgaaaaaag aggaaataag
      301 ggactaaaaa acagtactat atgccgtaac gcaggactaa taataaccga aagagctggt
      361 tcatcagtta aatagatttc ttgttgttcc agataaggca aacgagaaat tagcaacgca
      421 aaagcagcaa gagcactttt acgattccct tttaaagaaa gagaattgta tattccatca
      481 taaacgctaa agcccagttc ggtatgtttt gtagatttta tggtaaaatt gcctaaaaga
      541 tcgagattta ctttccaaaa ttcagtgctt tgttttttac cacactttat ttgtaattgc
      601 tgttcgctat gctgttgtaa agccaaaatg ctctgtaaat gtggttctga cactaaggca
      661 gaaattgtat ctccctcttt gggtatgcca ctaccgataa attcataggt cttatcttcc
      721 ttaacgagtt tgtagttaat aagatgtgaa agacgggtag ggctgccagg ttcaattccc
      781 aattgaactt gaaaatgcaa atggggaact gaagagcgtc ctgaattacc taattgagct
      841 atacaatcac ctttttgaac ataatcccca actttaattc tgatgctact ctgtttgaga
      901 tgggcataaa aagagtagag tccgtagcta tgagaaaagc agacataatt gccccaattt
      961 tctttagtat taatctggcc aataggatta tcaagaatac catcagaaat tttaactaca
     1021 taacctgctg ctgaagctaa aacaggtttc ccatacgaaa aatagtcatt cagattatta
     1081 tcttctttat cgtagcttct tttttcacta ttcaacattt caaaatccca agcataagcc
     1141 caatccaatt tatgagtata cataccatta ttaccttgag tgatcgtcca cagaccattc
     1201 aacggtatgg caaattgggg gatacctaaa tggctaaacc ttttatagcg ttcctgatag
     1261 gtctgcaaag ccaattcggg attgaaaaca ccataatcat tcattatagg tttacttact
     1321 ttcagccgta agcgtaaact gaatattact agtaaaacag ctatattgaa tggaaaagca
     1381 aaaaccggaa ctaacaaagg agtatataaa cccgtaaaag cattgtaatg ataatataca
     1441 acattgaaca gcacagtaaa taaaaaaccg atacctgttg ccaataaccc aattaaaata
     1501 gagcttttac ttggtaaaag atatattccc gcaactgcat acataattaa aatagcatta
     1561 aaacctgaat aaaaaactcc actacctggt tgcactccca tcaaacgcat taatatgaaa
     1621 tttagcgtcc accctaaaag tgccaaacca aatccaatac gcgtaataat tagtaaagct
     1681 aatgaaacta aaatgcctgc agtaacttct ggagtgaaaa atatagaacc caaagaaata
     1741 aagaataaac gccaaaattc gggtaactta ggtgctggac taaacagcag tattttatca
     1801 aagggataat tggataaata ccctttgcgg gcaaagtaat accatagcaa gatagctact
     1861 atggaaaaag ataaactcat agaaggtaaa cgaaataatt gatggaaaag ataactgcta
     1921 aacaggtata gtaaaagagt gagcaaagaa actaacaaca gaaaacaaag ataatttata
     1981 ggggcactgc tgataatctg cattggataa taatacccaa ccatcatacc tgttaaaagg
     2041 gaattgaaag cagttattcc actctttgat tcccagtgct caaatcctaa aatgcgtact
     2101 gccaaaattg ctacaattaa agagaataaa cccgataatc caacaacagg agatagtaat
     2161 gtaattgtca gcaacattaa acctaaacat accgaatccg aaaacaaaac cgtggcatag
     2221 ctatctggaa tagctaaaac cactgacccc acagacctga aaaatcgtgt ccagttcata
     2281 tcttccgtaa atgatctgga ataagttctc gggaagttat gtccgaaaga tcttcacgga
     2341 aacgaataac atctacttca ccggtttcgt ttattaaaca gacattagga cgatattcta
     2401 taaattgcat ccattgagtt acattataag caccaacagg agaaataata actgtatcgc
     2461 cataataaag atcaggaaaa ggcatagcac tgcgaaggac atcaatattc atacacagag
     2521 gaccataaaa tacggtgttt tgatatgttc ccggaaaagg tttggtaggt aatacgcgtt
     2581 gtttatacca ccaggcagta atgtttatat tcactcctgc atctaaaatt acagcacgct
     2641 caccagtggg aagattcttt ttacccagca cactggcaat aagataacca gattcgtcaa
     2701 ttaacactct tccagtttct aagattaaac ggggattgtg ttctttaaca taataagatg
     2761 cattaaaagc tgttcctatt gcttcagcat actgttccaa agagggtgaa gccaattctc
     2821 caggagtgta ttgatcaatt aaagtatttg aagaagcaaa accaccaccc atatctatat
     2881 actctaatac gataccaaaa ttatccttta tggtttgagc aaaattcaaa agtttggtgg
     2941 cagcccaata ataagcgttt ggatctaaaa taaaagtgcc aatatgagta tgcaaacccg
     3001 ctaagtgaat tttgcctccc gaaaccagac gctggacggt tctatacgct tcaccatttt
     3061 cataattaaa accaaaacgc gtccacattg gactgatccc tgtatccata tttacgcgaa
     3121 tagcaacttt gggcttgata gcaagttcat cagcaatatt ttctattaga tacagttcct
     3181 ctaagtggtc aatatgtata taggcacctt cttgtatagc taattgcaag tcctgcttac
     3241 gcttggaagg tccattaaaa tatattctat tcccagggat accattactc cttgcttttt
     3301 catattccat tctactaact acttctgctg ctgcaccttc ttgatgaaaa atattacaaa
     3361 tagctgcaag ataattagtt ttataactcc aaacaatttc tgctttggga taatgaatac
     3421 taattgtttc cagtaatcgc ctgtaggaat gtctaatatg tttctcagaa aagacaaata
     3481 gcggagttcc aaatttttct actaagttct taatgggaat accatcaata ttatcctgat
     3541 gtctaacaac cgtggtatgg gcatatttat taagatttcc agcgctattg cgttcaatgt
     3601 atggagcagt ataatgtttt ttcattttat ttcctcttgc tttttttgct ttctgatcag
     3661 ttcaccatta acagaaattt cacccagaag gttgatatta ctgataatat cttctgaatg
     3721 acggataaaa agaactcccg gtttaaaagg aggcaattct tcaatttgat tacctaaagc
     3781 cagtaagaca gtcgctgcag gttgattttg gccacatcct tccgctaaac gaacccaggc
     3841 cggaaaacga ggattaattt ccagtaaatg aaaaactccc tctttatctt taataatttc
     3901 cagttcgcag ggtcctcgcc aagaaagtgt ttttattact ttctgcgcaa atttttccag
     3961 agcaggattg tttactacta ctcctccaaa accttttccc ttatctgtta taaccagttt
     4021 tcgttgtgga accattccca aacagtttcc tttaccatct cctaaaataa ctacattaaa
     4081 ttcatcacct tcaataagct cttgcagaat aaccggtaag ccccattttt cctggagctc
     4141 cagaaaatac ttttgagctt ctataatatt attggcttta tatgcttcgt agagataacc
     4201 tttaataaaa actggaaact ctatttcttg gatagcttta ttgatttctg cgacactatt
     4261 caataggact gttttaggaa cagtaatatc ttcggatgta aaataatgaa acagtttgct
     4321 tttatcgcgc aggttaaatt gcttctctgt gcagataaaa gtatgtatgc caagttcttg
     4381 gagctctttt tgtaaacgaa tataaagcgg aatttctgaa tccaaagtag ggataataac
     4441 atcaatttta cttctggaat tgatataacc ca
//
LOCUS       DAORHB010000118         4450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_20842_length_4450_cov_5.605461, whole genome shotgun sequence.
ACCESSION   DAORHB010000118 DAORHB010000000
VERSION     DAORHB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20842_length_4450_cov_5.605461"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1252)
                     /locus_tag="PKM80_04300"
     CDS             complement(134..1252)
                     /locus_tag="PKM80_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNV93164.1"
                     /translation="MIDIIELRHNLHRIPELAFREFKTQALLEDSILKILQNQPENIW
                     KLHYFKNSTGLLLEYTCAKTPYLLFRADMDGLPITENTGVPYTSEHPGFMHACGHDVH
                     LSVLMGLIQVVTETQPQRNLLFLFQPAEEGEGGAKRVLEEGLIQKFDIEAAFAFHIAS
                     ELPVGTVSAKEGIFFAIPQEFDVCFYGKAAHIAFPEKGINALKAGIMFMNVVEERLSE
                     LQKQEKMIFHIGKMSSGTIRNIIADKCILEGTHRSLSRKTCQKINNILKETAEFVEKQ
                     TNTKSEVKYLVSYDPVINNANLVKRLQEICSSKKIEFIPAETAMTGEDFGFFTTLYPS
                     LLFWLGSGCDYPLHSDKFLPRDESVEVGIRIMSAFLKR"
     gene            complement(1252..2232)
                     /locus_tag="PKM80_04305"
     CDS             complement(1252..2232)
                     /locus_tag="PKM80_04305"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNV93165.1"
                     /translation="MKVAIVGATGEVGRMMITCLEESNLKVSELDLFASYRSAGTILY
                     YTDQPLTVQELKEDSLLTHYDYVLFSAGAGVAKNYAPIAAQTSELVIDNSSGFRQESD
                     IPLVVPQVNGELLIGYNGIVANPNCSTIQIILPLAVLDKLFNLNKLIVSTYQSVSGSG
                     HQGIETLMKQRKGTTDKGIYPQIIDLNVIPQIGGFLDSGYCQEEEKMHFESRKILSKW
                     DLAVCATTVRVPVVYGHSASVYAEFEDAVDIKAAETALQNAPGIVYYPNTYMTPLDLG
                     SSNDSHTCRLRLGTDDKSLAFWNVGHNVRLGAAANAVEILKTHAKYSGKL"
     gene            2374..2449
                     /locus_tag="PKM80_04310"
     tRNA            2374..2449
                     /locus_tag="PKM80_04310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2407..2409,aa:Arg,seq:cct)
     gene            2424..4325
                     /locus_tag="PKM80_04315"
     CDS             2424..4325
                     /locus_tag="PKM80_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNV93166.1"
                     /translation="MFDSSLGRQKISEKERLVDIPLYTFVAYFNKGILRFGFVTATKD
                     SYSEILTEDGTTTILSNTRFILTGKETLPNKNIVTFVSEVNEQAEQFEKTDFSFLADR
                     EYTLVEIVEELKLQSDVEIFALYKFLRAHPEHIKCKKHKYRLKTTEEIALFQIQQKEQ
                     EEEELFLQEVNAWFKGSELSIKNQHQLFCALPELQSDKKNKKLLELILANYPHHTPEQ
                     AILQFRKACGETPYYIDPIISNAGIPIGFSTLLNNEKLLPIQNSDPEETAFCIDDEST
                     KDFDDALSIAKDGSFWKVGIYVSSVSERLNFQGALFAEAKKRVSSLYTANAIIPLFPQ
                     QHSEKELCLLKNELRPVIALTLWLDSNFNIQRTEIARKKITISANYSYPEVDKHIAQE
                     PFATLFKFSKTLAEKREANSVPEEQRYYYYIVSTKKGLAVKCIDTQSPARKMVEELMI
                     VYNSSLAAYSVQENIPIIYRNINHIETLNTNLVISQAYLSTSPQFHPGIGVKAYMHTT
                     SPIRRYVDLINQTQILANLKGKTKPFSNEELENDIPRIEKTLLYLKETAHKSEHYWVL
                     EFLKQNYLNTPLDAYFRDGRNGFLRFELIPWGFVISAKCESYPLTDKLKIIIYKVDSD
                     QGFVWADLL"
BASE COUNT         1434 a          877 c          773 g         1366 t
ORIGIN      
        1 tacttagttc aaagtttgta gttcaaagtt cgtagttcaa agttcgtagt tcggtgatct
       61 cggtgatctc ggtggctaaa tatctaaaat cctctaaatc cttgtttcga tagttcaatc
      121 tgtgagagag gtattatctt ttcaagaaag cgctcataat ccttattcca acttctacgc
      181 tttcatctcg aggtaaaaat ttatccgaat gtaagggata atcgcatccg cttcccagcc
      241 agaaaagcaa tgagggataa agagtagtaa aaaagccaaa atcttcacct gtcatagcag
      301 tttctgcagg gataaattca atttttttgc tactgcatat ttcctggagg cgtttaacta
      361 aatttgcgtt attaattacc ggatcgtagg agacgaggta tttcacttca ctctttgtat
      421 tagtttgttt ttctacaaat tctgcagttt cttttaggat gttattaatt ttttggcagg
      481 tttttctgga aagcgagcga tgtgttcctt ccagaataca cttatcggca ataatattac
      541 gaattgttcc cgagctcatt ttgccgatat ggaagatcat tttttcctgt ttctgaagtt
      601 cagataagcg ttcttccact acattcataa acataattcc tgcttttaga gcatttatcc
      661 ctttttctgg aaaagcgata tgagctgctt tgccataaaa acaaacatca aattcctgag
      721 gaatggcaaa aaagatacct tctttagccg aaacagttcc aacgggcaat tcacttgcta
      781 tatgaaaagc aaaggcagct tcaatatcaa acttttgaat taggccttct tccaaaactc
      841 tttttgctcc tccttctcct tcttctgctg gctggaaaag aaagagcaaa tttcgctgag
      901 gctgggtttc tgttacaact tgaattagcc ccatcaaaac acttaaatga acatcatgcc
      961 cacaagcatg cataaaacca ggatgttctg aagtataagg tacacctgta ttttctgtaa
     1021 tcggtaagcc atccatatcc gctctgaata atagataggg tgtttttgca caagtgtatt
     1081 ccaaaagcaa gccagtgctg tttttaaaat aatgcaattt ccagatattt tccggttgat
     1141 tttgcagaat ctttaaaata ctatcttcca gcagcgcttg tgttttgaat tctctaaatg
     1201 ctagctcagg aatccgatgt aagttatgac gcagctcaat tatatcaatc atcaaagctt
     1261 acccgaatat tttgcatgcg tttttagaat ttccacagca ttcgcagcag ctcccaaacg
     1321 cacattatga cccacattcc aaaaagctaa ggacttatca tccgtcccta accgtaaacg
     1381 gcaagtgtgt gaatcattgg aagaacccaa atccaaaggc gtcatataag tatttggata
     1441 atagacaatt ccaggtgcat tctgtaaagc tgtttctgca gcctttatat ctactgcatc
     1501 ttcaaattcg gcataaacag aagcactgtg tccataaaca actggcaccc gaacagtagt
     1561 agcacataca gctaaatccc atttagatag tatcttacgg ctttcaaaat gcattttttc
     1621 ttcttcttgg caatagccac tatccagaaa accaccaatt tgtggaatta cattcaaatc
     1681 tattatttgc ggatatattc ctttatcagt tgtgcctttg cgttgtttca tcaaagtttc
     1741 aattccctga tgtccgcttc cagaaactga ttgataagta gaaacaatta acttatttaa
     1801 attaaagagc ttatccaaca ccgccaaggg caaaataatc tgaattgtag aacaattagg
     1861 attggccaca ataccattat aaccaataag caattctcca tttacttgtg ggacaaccag
     1921 aggaatatct gattcctgtc tgaagccaga agagttatct attaccaatt cagaggtctg
     1981 agctgcaatc ggagcatagt ttttagcaac tcctgcacct gcagaaaaaa gaacatagtc
     2041 ataatgtgtt agcagagaat cttctttcag ttcttgaact gtaagtggtt gatcagtgta
     2101 atataaaatt gtacctgcag agcgatatga agcaaataaa tctaattctg acacctttaa
     2161 attgctttct tccaggcagg taatcatcat tctgccaact tcacccgttg cgccaacgat
     2221 tgctactttc attttttgtt tacctgtttt tccttatttt atgtatctta atcttatgat
     2281 cctaaagtgc attcactttt tctgtgaagg gatagcttgt caatgtaaac ttgacaaaaa
     2341 aaggctctta ccagattggt gaacaataaa agcgccccag tagctcagtg gatagagcag
     2401 cggtttccta aaccgttggt cggatgttcg actcatctct ggggcgccag aaaattagtg
     2461 agaaagaaag attggtagat attccacttt atacttttgt tgcctatttt aataagggaa
     2521 ttctgaggtt tggctttgta actgctacaa aagatagtta ttccgaaatt cttacagaag
     2581 atggaacaac tacaattttg agtaatacgc gttttatttt aacaggtaaa gaaacacttc
     2641 ccaacaaaaa catagttacc tttgtatccg aagtaaatga acaagctgag cagtttgaaa
     2701 agaccgattt cagcttctta gcagaccgag aatatacttt agtggaaata gtggaagaac
     2761 ttaaactgca atctgatgta gagatttttg ccctatataa atttctacga gcacaccccg
     2821 aacatatcaa atgtaaaaag cataaatatc gtttgaaaac aacagaagag atagctctat
     2881 tccaaattca acaaaaagaa caggaagaag aagagctttt tttgcaggaa gttaatgcct
     2941 ggttcaaggg ttctgaactt agtatcaaaa atcaacatca gcttttttgt gccttaccgg
     3001 aactgcaatc ggataaaaaa aataaaaagt tactagaatt gattttggcc aattatcctc
     3061 atcatacacc agaacaagca attctgcaat ttcgtaaagc ttgcggtgaa actccatatt
     3121 acatagatcc tattatttct aatgcaggga tccccatcgg cttttcaacc cttttaaata
     3181 atgaaaaatt actcccgatc caaaattctg atccggaaga aactgctttt tgcattgatg
     3241 atgaatccac caaagatttt gacgatgccc tcagtattgc caaagatggc tctttctgga
     3301 aggtaggaat ttatgtaagc tctgtttcag aacggctaaa ttttcaagga gccttgttcg
     3361 cagaagcaaa aaagagagtt tcttcattat atacagcgaa cgccattatc cctttatttc
     3421 ctcaacagca ttcagaaaag gaactctgtt tgctaaaaaa tgaactaaga cctgtaatag
     3481 ctttaacttt atggctggat agtaacttta atatccaacg caccgaaata gccagaaaga
     3541 agataactat ctctgctaat tatagttatc ctgaagtaga taagcatatt gcacaagaac
     3601 cctttgcaac cctattcaaa tttagcaaaa ccttagcaga aaaaagagaa gcaaactcag
     3661 ttcccgaaga acaacgatat tactattata ttgtatccac taaaaagggc ttggcagtta
     3721 aatgtatcga tactcaaagt ccagccagaa aaatggttga agaacttatg attgtatata
     3781 attcctcttt ggcagcttac tcagttcaag aaaatatccc tattatctat cgtaatataa
     3841 atcacattga aaccctgaat actaatcttg ttatcagcca ggcttatctt tccacttccc
     3901 cacaatttca tcccggaatc ggcgttaaag cctatatgca tacaacttcc ccaatccgcc
     3961 ggtatgtaga tttaataaat caaacccaaa tcttagcaaa cctgaaaggg aaaacaaaac
     4021 cttttagcaa tgaagaactg gaaaatgaca tcccccgcat cgaaaaaacc ctgctctact
     4081 taaaagaaac agctcataag agtgaacatt attgggtgct ggaattttta aaacaaaact
     4141 acctaaacac cccactggat gcttatttca gagatggacg caatggcttt ttgcggtttg
     4201 agcttatccc ctggggtttt gttatatctg caaaatgcga aagctaccca ttaacagata
     4261 aattgaaaat tataatttat aaagtggatt ctgatcaagg atttgtttgg gcagatttgc
     4321 tttaagaaac aaggatttta gggttagatt ttagaaacaa ggatttaagg attttaggat
     4381 ttggaggatt tcttctttaa agttccgcta ggaacgatac atactaacga cgggttttta
     4441 acccggcgat
//
LOCUS       DAORHB010000119         4368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_21445_length_4368_cov_6.302342, whole genome shotgun sequence.
ACCESSION   DAORHB010000119 DAORHB010000000
VERSION     DAORHB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21445_length_4368_cov_6.302342"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2734)
                     /locus_tag="PKM80_04320"
     CDS             complement(<1..2734)
                     /locus_tag="PKM80_04320"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV93167.1"
                     /translation="MIDDELIFGSDFFDLEEELAPTSDKLHLREGELREVTVLFADIH
                     GFSSISNLFDAETIHRRMDEIMKIFSRCISFYGGFVDKYMGDGIMALFGAKTATEQNT
                     ERAILAALKMQEQLQLYNKKLKQQPDFENVDLGVRIGINAGLVSVGKVGEDREGDFTV
                     YGPEVNLASRMESNAPVNRIMLPEKTMLAVQRNFEFEDVGLKSVKGFSEPLPCYLVIG
                     PKLDSSLHRRNLSTRYIGRIKEIDLLKETFSEIGKQQQISVVAIQGEAGIGKTRLVYE
                     FERTCADNTIFLQGACSAISPTPLNLFTSLCETLFRLQINEKPEVKRKKLDNAYNILW
                     DIASEEEKELLSDGKNLIAFLLELKSEDSRLKQSGADLLQHLSLAIENLIMAQINYAL
                     RSNRKLVIVLDDLHWLDEASAKVMENLLNKIASAGALPPLMLILMFRPGYNLASYFQV
                     LTQVKIIELNPLAAEEIRELIAAQAKDKHLLEDTVDKVIQLSQGNPFFVEEWCNYIED
                     LPLDELKDIPVPANLYNLILSRLDKLPVSLRMMLHKAAVIGQEFFVEILRYMEKRLQD
                     NLDVDATLKSLEDQSYILHSLGFDYSTYFFKHILTREVAYQTLLAENRKVLHRLCGEA
                     IETLYPDRLDEFYYALAEHFHKAEVIDKAIIYIEKAANSAAKVYNNQQALRFYQLLLD
                     YPDLQPVKRLEITMQIADIMWLTGDWNNALKIVYDLLHEAQKINAEKVCFEAYRFLGI
                     AAFYQNEKDEALSYFKLAYDLAKKLNDPALISIAVGNLGNWYFRTGDLTLASHLQQQD
                     LSISIDRQDSKRQAKAYSNLGLICLEEDDLEKAEDYFYKSLKIAVDNRFLKEQSIALG
                     NLGYTKMLAENYAEAFPLLQQKLKIATEMNDKLELINVLGNIAIYYMEIGERNMA"
     gene            3038..3457
                     /locus_tag="PKM80_04325"
     CDS             3038..3457
                     /locus_tag="PKM80_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV93168.1"
                     /translation="MTQINCKIVKETVATAILALYRQAGWWSTEDKDSDLEIITKIVA
                     NSFCFVIATLDDQIVGMGRALSDGISDAYIQDVTVQKEFRGQGIGKAIIRTLVSFLKE
                     HKLQWIGLICEPGYEGFYQGLGFKVMASYTPFLYSET"
     gene            3458..4339
                     /locus_tag="PKM80_04330"
     CDS             3458..4339
                     /locus_tag="PKM80_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNV93169.1"
                     /translation="MNGLQLITEEHIPLLQDFLTRYPRRNCDFNVTNLLAWGTIYRNH
                     YLLWQDNLVIFNPKYQNVCFPLGDNFTPAELANLVRLFKQEYPEAELIIVPEEYYAQT
                     PELDNYFEVSDDRAWADYVYEIDSLVKLSGKKLAKKKNLISQFIRAYPDYQVLPVTND
                     KLDALLRFTYKWKRERSAEGIYLMTELKAIENTLAMWDILPCEGIIICLHDKIAAYSI
                     FSPQTPDMADEHFEKFDPDKKGSAQIVNWETARHLQNRYKYLNREQDLGMEGLRQAKL
                     SYQPAFLVKFLSTKLKA"
BASE COUNT         1373 a          843 c          750 g         1402 t
ORIGIN      
        1 ttgccatatt tctttctccg atttccatat agtaaatagc aatattacct aacacattga
       61 tcaattcgag cttatcattc atttctgtag caatttttag tttctgctgc aaaagaggga
      121 atgcctctgc atagttttca gccagcattt ttgtgtaacc caaattccct aaagcaatag
      181 attgttcttt tagaaagcgg ttatctactg ctatttttag ggacttataa aagtaatcct
      241 ctgccttttc caaatcatct tcttccaagc atataagccc caaattacta tatgcttttg
      301 cctggcgttt actatcctgt ctatctatag atatagatag atcctgttgt tgcaaatggg
      361 aagcgagagt taaatctccg gttctaaaat accagtttcc taagttccct acggcaatag
      421 agattagagc agggtcattt aactttttag caagatcata agcaagttta aaataactta
      481 gggcttcatc tttttcattt tgatagaacg cagcaatccc taaaaaacga tatgcttcaa
      541 aacatacttt ttcagcattt attttctggg cttcgtgtaa caaatcgtaa actattttaa
      601 gagcattatt ccaatcgcct gtcagccaca taatgtcggc aatttgcatt gtaatttcca
      661 aacgcttcac tggttgcaaa tcaggataat ctaaaagtag ttgataaaaa cgcaaagcct
      721 gttgattgtt atatacctta gctgcactat tagctgcttt ttctatataa ataatggcct
      781 tatctataac ttctgcttta tgaaaatgtt ctgccaaagc atagtaaaat tcgtccaaac
      841 ggtctggata caaggtttca attgcttctc cacataaacg gtgaagcact ttacggtttt
      901 ctgccagtaa agtttgatag gcaacttcgc gggttaaaat atgcttaaag aagtaagtgg
      961 aatagtcaaa ccccaaagaa tgtaaaatat aggactgatc ttccagagat tttaaagtgg
     1021 catctacatc cagattatcc tgcaagcgtt tttccatata gcgtaaaatt tccacaaaaa
     1081 attcctgacc tatcactgca gctttatgta acatcattct taaagagaca ggcagtttat
     1141 ctaaacgcga taagataaga ttatacaaat tagcgggaac cgggatatcc tttaattcat
     1201 ctaggggtaa gtcttcaata taattacacc attcttccac aaagaaagga ttaccttgag
     1261 agagttgaat caccttgtct accgtatctt ctaaaagatg tttatccttt gcttgggcag
     1321 ctattaattc ccttatttct tctgctgcta aaggatttag ctcgataatc ttcacttggg
     1381 ttagaacctg aaaataggaa gccaaattat atcctggacg gaacatcaat attagcatca
     1441 aaggaggaag cgctcctgca gaggcaattt tattcaataa attctccata acttttgcag
     1501 aagcctcatc cagccaatgc aagtcatcta aaactattac caacttgcgg ttactgcgta
     1561 aagcataatt aatctgagcc attatcaaat tttcaatagc taaagaaaga tgctgtaaaa
     1621 ggtctgctcc gctctgtttt aaacggctat cttcactctt caattccagc aaaaaggcaa
     1681 tcaggttttt cccatcagaa agcaattcct tttcttcctc tgaagctata tcccacaata
     1741 tgttataggc attatccagt ttttttcgct taacttcagg tttttcattt atctgcaacc
     1801 gaaaaagtgt ttcacatagc gaagtgaata aatttaaagg agtgggacta attgcactgc
     1861 aagcaccctg taagaaaatt gtattatctg cgcaggttct ttcaaattca taaaccaggc
     1921 gtgttttccc tattcctgct tccccttgaa tagccacaac cgaaatctgc tgttgtttgc
     1981 caatttcaga aaaggtctct ttcagcagat ctatttcttt tatcctgccg atataacgag
     2041 tacttaaatt cctgcgatgt aaagaactat ccaatttggg gcctattact aaataacagg
     2101 gaagcggttc agaaaaacct tttacacttt ttaaacctac atcttcaaat tcaaaattgc
     2161 gttgaactgc cagcattgtc ttctccggaa gcataattct attcactgga gcattggatt
     2221 ccatccttga agctaaatta acctcaggac catacactgt aaaatctccc tctctatctt
     2281 cgccgacttt tcctacagat acaagaccag cattaatccc aatccgaacc cccaaatcta
     2341 cattttcaaa atctggctgc tgttttagtt ttttattata tagttgtaat tgctcctgca
     2401 tcttcagcgc tgctaaaatt gctcgttcag tattttgctc agttgcagtt ttggcaccaa
     2461 ataatgccat tatgccatct cccatatatt tatctacaaa accaccataa aaactaatgc
     2521 agcgtgaaaa tattttcata atttcatcca ttctgcggtg aatggtttcg gcatcaaaaa
     2581 gattggatat actactaaag ccatgtatat ctgcaaaaag tacagttact tccctaagtt
     2641 caccttctcg taagtgaagc ttatccgaag taggagcaag ctcttcttcc agatcaaaaa
     2701 aatcgctgcc aaaaatcaat tcatcatcta tcattatgct gctatccttt ttattctcat
     2761 tatacaataa tagctgaata tatatattct gtcaacctca aactgaaaag aagagggttg
     2821 ggttgaagag caaaatatat agcttgggag ctcaaagcta agtatttctt tctttgttcg
     2881 gtgttttcga tggctaaaaa atacatctgt gaaaactgcg taatctgcaa gaaacaattt
     2941 cttctttaaa atgcgtgctt gacaaaaatc ccccttcatc cagatagttt ttttaaggaa
     3001 agaccctaca caatgtatat agggcaaaca tataattatg acacagataa actgtaaaat
     3061 tgttaaagaa acagttgcaa cagccatttt ggcactctat cgtcaggcag gatggtggtc
     3121 aactgaagac aaagattccg atttggagat aattacaaaa attgtagcta attcgttttg
     3181 ttttgttatt gctactttag atgaccagat tgtcggtatg gggcgagctt taagcgatgg
     3241 tataagtgat gcttatattc aagatgtaac cgttcaaaag gaatttagag ggcaaggaat
     3301 cggcaaagca ataatccgca ctttagttag ctttttaaag gaacataaac tgcaatggat
     3361 agggctaatt tgtgagccag gttatgaggg tttttatcag ggcttgggct ttaaagtgat
     3421 ggcctcctac actccatttt tatatagcga aacatagatg aatggcttgc aattaattac
     3481 agaagagcat atacctcttt tgcaggattt tttaacccgc tatccaagaa gaaattgtga
     3541 ttttaatgtt accaatcttc ttgcttgggg gacaatttac cgaaatcact atctgctttg
     3601 gcaggataat ttagttatct tcaatcccaa ataccaaaat gtctgtttcc ctttaggaga
     3661 taattttacg cctgcggagt tagcaaattt ggttcgtctt tttaagcagg aatacccgga
     3721 agcggaattg ataattgttc cggaagagta ttatgctcaa actccagaat tggacaacta
     3781 ttttgaggtg agcgatgata gagcttgggc agattatgtt tacgagatag atagcttagt
     3841 aaaactatcc ggtaagaaac tggctaagaa aaagaacctt atttctcaat ttatccgtgc
     3901 ttatcctgac tatcaggttt tgcctgtaac caatgataaa ttggatgcat tattgcgttt
     3961 tacttataaa tggaaaagag agcgtagtgc cgaaggtatt tatttgatga cggaacttaa
     4021 ggctatagaa aatactttag caatgtggga tattttaccc tgcgaaggta ttattatttg
     4081 tctgcacgat aaaattgctg cctattctat attttcacct caaacccctg atatggcaga
     4141 tgaacatttt gaaaaatttg acccggataa aaaaggttca gcacaaattg ttaattggga
     4201 aacagctcgc catttacaaa atcgttataa atatctgaat agagagcaag atcttggtat
     4261 ggaaggttta agacaggcca aattatctta tcagccagca tttttggtta aatttctttc
     4321 taccaaatta aaagcttaat ccacccagag caaattatag aaatacga
//
LOCUS       DAORHB010000120         4359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_21519_length_4359_cov_5.215381, whole genome shotgun sequence.
ACCESSION   DAORHB010000120 DAORHB010000000
VERSION     DAORHB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21519_length_4359_cov_5.215381"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            461..904
                     /locus_tag="PKM80_04335"
     CDS             461..904
                     /locus_tag="PKM80_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNV93170.1"
                     /translation="MVIAAEIIAQKMYQALEKSFAKPETSSVFKELVILEKMHEEKMR
                     LVFAKQFPGQEIVFSEEPVKIIPKVDLNDPKEVLDFAISKEEESIELYKSMAGQTTEP
                     EIKEQLLQFANEEEGHKSILLTEIQRLQGALEWFDPSELTGLMED"
     gene            914..2227
                     /locus_tag="PKM80_04340"
     CDS             914..2227
                     /locus_tag="PKM80_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNV93171.1"
                     /translation="MVNKDQTTLFDKDELSNKTVPLAEKLRPKTLEELLGQTKLIAGN
                     SPLRQMIESGEYHSFILWGPPGSGKTTIARIIEQNSDYHFIHFSAVLASISDVKAVMK
                     EADYLHRTQNHRSILFIDEIHRFNKSQQDAFLPYVESGAIILIGATTENPSFEVIPAL
                     QSRCTVFVLEPLSESDLKIILQRGFAELKLEEDEDIISWMAQNAGGDARRALNDLELV
                     LPFMKKKPHPSIDELAKFLAKKTMFYDKNREEHYNLISALHKSLRGSDPQAGLYWLAR
                     MLEAGEDPRYIVRRLIRFASEDIGLADPNALVQAIAAKEAILFIGMPEASNALAQLVV
                     YLATAPKSNSLYTAYAEAAEDAKKTSHYGVPLHIRNAPTKLMKDLNYGKNYHYDHEYE
                     NKYYYQKYFPDQMPEKIYYKPGDYGFEKEIQKRIDWWAKLKKEQK"
     gene            complement(3044..>4359)
                     /locus_tag="PKM80_04345"
     CDS             complement(3044..>4359)
                     /locus_tag="PKM80_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNV93172.1"
                     /translation="TSTKGAFGGLGIQIDKIGDYITVVSPIEGTPAYRMGITAGDKIV
                     KVNGESMVGLSTDEAIKRMRGEVGTKVQITISRPGLAEPIDFEITRETIKIKSVPYSF
                     KLDNGVGYIRLTQFSENTLSELRAALNNLESEGIRGLIIDLRWNPGGLLDQAVDTVNE
                     FIGQNRLVVETKGRTENKQLYTRYAVKERNYPVIVLVNEASASASEIFAGSLQDWDKG
                     LVMGKNTFGKGSVQQLIPLSNGNGIKITTAYYYIKSGRCIHKMSNDNILKGKEVSTAD
                     LEQESELNRKQVYYTANNRKVYGGGGIQPDIVVESDFLTLFGVELRRKNVFFNFAVDY
                     LVAHNHQFDEKAEISDKTMNDFLNYASKNGIKYTQADVDSADSFIRTTIKSELVRKVK
                     GDAEAYKITISQDKQLMEAIALFDRFKTLDQMFSYAASLNGKKEK"
BASE COUNT         1376 a          773 c          862 g         1348 t
ORIGIN      
        1 tgtgttataa cttgtccaca tcactttttt gtgggaatgc cttaaaaatc agataagtcg
       61 aagtatctta tcaactcact gccatagttg ttttcttaag ataattaata ggatcatagc
      121 actgatgtcg gtgatagaat atatcatcca taatatgtta ccgtcattat gaatgcgcat
      181 cgttagacaa aatataggtt tatcttaaat tatcgtagcg atgataactc ctaactttcc
      241 ataagattga ataatattaa tgaagctaat gattaatttt cgaaatatct taaagctcgg
      301 ttggctggag aacgaagagg acatcgttca tcctgcatac agattactgc attctgtatt
      361 ttttcacccc tttcaacctt ttcttgcttt aagccacaat ttcgattatt atattataag
      421 caaggaggat aaaaaaatga cattagaaga tagttatcgg atggttattg ctgctgaaat
      481 catagcgcaa aagatgtatc aggctttaga gaaaagtttt gccaagccag agaccagcag
      541 cgtttttaaa gagctcgtaa tattggaaaa aatgcacgag gaaaaaatgc ggcttgtttt
      601 tgctaagcaa tttcctgggc aggagatagt cttttctgaa gagccggtaa aaataatccc
      661 gaaggtagat ttgaatgatc ccaaggaagt actagatttt gccattagca aggaagagga
      721 atcaatagag ctctataaaa gcatggcagg ccaaactact gagccggaaa ttaaagaaca
      781 attattgcaa tttgccaatg aagaagaggg gcataaatct attttattga ctgaaattca
      841 gcgtttacaa ggtgctttgg agtggtttga tccttctgag ttaactggat tgatggagga
      901 ttgaacatat tttatggtaa ataaagatca gacaaccctg tttgataagg atgaactcag
      961 caacaaaact gtacctttgg cagaaaaatt aagacctaaa acgctggaag aactattggg
     1021 tcagacaaaa ttgatagcgg ggaattcacc attaaggcag atgatagaaa gtggggagta
     1081 tcattctttt attttatggg gacctcctgg aagtggcaag acaaccatag cccgaataat
     1141 tgaacaaaac agcgattatc attttattca tttttctgca gtccttgcca gcatcagtga
     1201 tgtaaaagca gtaatgaagg aagcagatta tcttcatcga acacaaaatc atcgtagtat
     1261 tttgtttatt gatgaaattc atcggtttaa taaaagccaa caggatgcct ttttacctta
     1321 tgtagaaagc ggggctatta tattaattgg agcaactaca gaaaatcctt cttttgaagt
     1381 aataccggct ttacaatccc gatgtaccgt ttttgtttta gagcccttaa gtgaaagtga
     1441 tttaaagata attttgcagc gtggttttgc cgaattgaag ctggaagaag acgaagatat
     1501 tattagttgg atggctcaaa atgcaggagg cgatgcgcgt agagctctaa acgatttgga
     1561 gctggtttta ccttttatga aaaagaaacc gcatccgagc attgatgaac tggcaaaatt
     1621 tctggctaaa aaaacaatgt tttacgataa aaatcgcgaa gagcattata acttaatttc
     1681 tgctttgcac aaatcgttac ggggttcaga ccctcaagct ggactatact ggctggcaag
     1741 aatgttggaa gcaggagaag accctcgata tattgtccgt cggctgattc gttttgccag
     1801 tgaagatatt ggcttagcag atccgaatgc cttagttcaa gcaatagcag ccaaagaagc
     1861 aattcttttc atcggaatgc cagaagcaag caatgcttta gctcaattgg tagtatattt
     1921 agctacagcc ccaaaaagta actctcttta tactgcctat gcggaagcag ccgaagatgc
     1981 taaaaaaaca agccactacg gagttccctt gcatatcaga aacgcgccta caaagctgat
     2041 gaaagacctg aattatggaa agaactatca ttacgatcac gaatatgaaa ataaatatta
     2101 ttaccagaag tattttccag accaaatgcc agaaaagata tattataaac ctggcgatta
     2161 cggttttgaa aaggaaattc aaaaacggat tgactggtgg gcaaaactta aaaaggaaca
     2221 aaagtagaaa gctttgtaca gtgcaaattt aatagtaatt atcaattcta cccgataggc
     2281 attttgtcgt tgcacataca atcaagtttt gtagttattg atattatata taggagtgag
     2341 gaaagtagtt tgtgttgcta atcagattag ctacagaata aaaaacccct tcgtttggaa
     2401 ggggtttagg tctttgactt aggaggtcgc acattaaatg acctttgggg gaaaggtcat
     2461 taaccggcaa gggggcgccg gttatttgag attcattaaa tacaagtaag catatcctat
     2521 accaaatatt gattctctgc tatatgttta ttaaatatga agatagctaa tcatgctgtt
     2581 atttttggct gatgtatttg tagaaaacaa tacacatatt gttgaataag atctacagtt
     2641 attggtagat tgcagcattc tatcgttgtg ctcagctaat agttgactta atattttgga
     2701 gcccaaatat gtctcctacc taattagcta tagtggaact gaggtgaggc tacggagcaa
     2761 aatcccaagg atgcaccatt tgattcccac cgtagctaat taaggaagaa tcaaactaca
     2821 tttcgaattt cgattataag gcaaataagg caaagctagt agatgtttag cacaataagc
     2881 gaaaaggata tacaattcta aggcaacaat tagccttcag cgaacttatg ttgtttgcat
     2941 tatcaaccgg aacacaatgg aattttctat tccattgtgt attatacttc tgaagataag
     3001 ctatagatcg ctatccaatc agttatttaa gctgttcggt tgtttacttt tcctttttcc
     3061 cattcaggga agctgcatag gaaaacattt gatctaaggt tttgaatcgg tcaaacagag
     3121 caatagcttc cattaactgt ttatcttgcg agatggttat tttatatgct tccgcatcgc
     3181 ctttaacttt gcgtacaagt tcgcttttga tggttgttcg gataaaggaa tcagcgctat
     3241 ctacatctgc ttgagtatat ttgatgccat ttttggaagc atagttaaga aagtcgttca
     3301 tagttttatc gctgatttct gctttttcgt cgaactggtg gttatgggca accagataat
     3361 ccactgcgaa attgaagaaa acattttttc gacgcaattc tactccgaaa agagtgagaa
     3421 aatcgctttc gacaacaata tcgggttgaa ttccgccacc gccatatacc tttcgattat
     3481 tagcagtgta ataaacttgt ttacggttta gttcagattc ttgttccagg tctgcggtac
     3541 taacttcttt acctttcaat atgttatcgt tgctcatttt atgtatgcag cgtccgcttt
     3601 tgatatagta ataagcagta gtaattttaa taccatttcc attggaaaga gggataagct
     3661 gttgtacact gcctttcccg aaggtattct tacccataac caaaccctta tcccaatctt
     3721 gtaaacttcc ggcaaaaatt tcggaagcac ttgcagaagc ttcattaact aaaacgataa
     3781 caggataatt gcgttcttta acggcatagc gagtataaag ttgtttattt tcggtgcgtc
     3841 ctttagtttc tacaaccaaa cgattctgtc cgataaattc gttaactgta tctactgctt
     3901 ggtctagaag gccaccagga ttccaacgca ggtctataat taatcctctg atgccttcgc
     3961 tttctaagtt attcaaggca gcgcgcagtt cggaaagtgt attttcgcta aattgggtta
     4021 ggcgaatata tccgactcca ttatcaagct taaagctata aggcacgctt ttgattttta
     4081 tagtttcgcg ggtaatttca aaatctatgg gttcagctaa tccaggacgg ctgatagtta
     4141 tttgaacttt tgtgcctact tctcctctca ttcttttgat tgcttcgtcg gtagataggc
     4201 caaccataga ttctccatta accttcacga ttttatctcc ggcagtaatt cccatcctgt
     4261 aagcaggtgt accttctata ggagaaacca cagttatata atcaccaatc ttatcaattt
     4321 gaattcccaa tccaccaaaa gcacctttag tagaagtag
//
LOCUS       DAORHB010000121         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_21631_length_4345_cov_4.161538, whole genome shotgun sequence.
ACCESSION   DAORHB010000121 DAORHB010000000
VERSION     DAORHB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21631_length_4345_cov_4.161538"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PKM80_04350"
     CDS             <1..488
                     /locus_tag="PKM80_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNV93173.1"
                     /translation="KGDFTQMLKKSFTEVSFWDINLNGKQFHTILKDKQVHPVSRDIL
                     HIDFMVIAAEALIEIEVPISFIGEALGAKEGGMVEIVQRSVKVSCKANEMPEDLTLDI
                     TNLKIGDSLHIRHLPKGNWQFKEPDDVTLVTVHSLKVETPVEPTAAVTPEEHTTPEPE
                     K"
     gene            498..1094
                     /gene="pth"
                     /locus_tag="PKM80_04355"
     CDS             498..1094
                     /gene="pth"
                     /locus_tag="PKM80_04355"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNV93174.1"
                     /translation="MKLIIGLGNPTREFEHNRHNLGFICLDRWAADKGRKFAKGEIFD
                     YIFMHRAVLIKPRTYMNSSGEAVQKAMACWKITDSMVIYDDIELPTAQIRIRQGGGDG
                     GHNGIKSLLTVIPPEELKRIRIGIGRNKEVEPRDYVLSDILPEEWDLLNPTLDVVGKF
                     IDMYIKYDFNAVLNEYSIWKKSYSGADSSGIISPKEES"
     gene            1094..1381
                     /gene="rpsF"
                     /locus_tag="PKM80_04360"
     CDS             1094..1381
                     /gene="rpsF"
                     /locus_tag="PKM80_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNV93175.1"
                     /translation="MIKDYELMLILNPNQSADEANAMNDSLVALVTENGGEITKIDPW
                     GKRMLAYPINKFNEGYYYVDYFKLDSLLVKKLSASFKINENIIRHQFVVKN"
     gene            1392..1844
                     /locus_tag="PKM80_04365"
     CDS             1392..1844
                     /locus_tag="PKM80_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNV93176.1"
                     /translation="MADLKLPRLNKCIFSGRIGNDIELKYTPKGTPVVRFVVACDRRY
                     KDDSEQWQTVTSWIECVAWNKWAEFIANQVHKGSPVIVEGRIETRNWTDQNNQNRKTT
                     EVIVEVIHNLEWKPREGEASAVSEDVPLPAEETAPHNATNTTTDDVPF"
     gene            1859..2089
                     /gene="rpsR"
                     /locus_tag="PKM80_04370"
     CDS             1859..2089
                     /gene="rpsR"
                     /locus_tag="PKM80_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNV93177.1"
                     /translation="MNDRKKKYSRKKYCKFCANKDIQIDYKNIDMLKQYISEVGKIEP
                     ARLTGTCAKHQRQLAREIKRARQMALIPYVIE"
     gene            2090..2926
                     /locus_tag="PKM80_04375"
     CDS             2090..2926
                     /locus_tag="PKM80_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93178.1"
                     /translation="MQIFPLIFGAISLLSPFVALIVYMMYIGKTAEVHLTKPLQAFLV
                     FIPVPVLILLFDPQTSSLLLGLDAIFAVGLPVLIFLLTLKHNHILSNAFLAAYLVIAA
                     WGILRYYLFQTYQNQLFEQGLQMVKDRLPALMDKSLTDQTLPIWKAIIPAIWVVSQSI
                     AWLIGFLFFEKNLQIPNKITNLRFPVYYNILIMAVLPLYFFDQTKVLFYNLLITLCVI
                     PFIQGASLVWQRLSLIFTNRFISGLFMIIIVLYANILLVLIGFANMWFSKRNLTIEGD
                     AA"
     gene            2923..3369
                     /gene="rplI"
                     /locus_tag="PKM80_04380"
     CDS             2923..3369
                     /gene="rplI"
                     /locus_tag="PKM80_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNV93179.1"
                     /translation="MKVIMLKTIEKVGKQGEVVNVKRGFARNYLVPKKLAIYATPANL
                     KNLANIQSKLADEEQKLMAELKNLAEKIDTTNLVFKRKVDEQGSMFGSVSELDIINGL
                     QELGIQINKSALSMEKHYKELGEFNLPVRLHKDIICSLNIKIEPEQ"
     gene            3388..3963
                     /locus_tag="PKM80_04385"
     CDS             3388..3963
                     /locus_tag="PKM80_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93180.1"
                     /translation="MSDLGYKIKQILSLIVFVAVLSLLGMISGRPIMILAYGVFFLVV
                     VAIMFYLTNKRQRHFEKVKESSPVFKKVFGIILVLLSLITPMVIIFKTNLVNLPESLP
                     IGTAIAIVGAVSIVFIALTLAAVYFINMRGKQVSNRVIGYILYVIAAMIPGLLMSRIE
                     KTTIGIGSVYYVALIVLILAYSGFGLITNRE"
     gene            4032..>4345
                     /locus_tag="PKM80_04390"
     CDS             4032..>4345
                     /locus_tag="PKM80_04390"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNV93181.1"
                     /translation="MSFSSISKLSGQLVYQLGGDKYKNFIHIYLSWKKVVGELLASKS
                     YPIKIENKVLFVTVHNNTWMQELILIKDDIISKYKNIYEEEIADIVFLISSPKGKCKR
                     RK"
BASE COUNT         1416 a          695 c          875 g         1359 t
ORIGIN      
        1 ccaagggtga ttttactcag atgctcaaaa agagttttac tgaagtgagc ttctgggata
       61 taaacctaaa tggtaaacaa tttcatacta tcttgaaaga caaacaagtg catcctgtta
      121 gtagagatat tttgcatatt gactttatgg ttatagcagc agaagcccta attgaaattg
      181 aagttcctat tagttttata ggagaagcac ttggagcgaa agagggcgga atggtagaaa
      241 tcgttcaacg ctctgtaaaa gtatcctgta aagcaaatga aatgccagag gatttaactt
      301 tagacatcac taatttgaaa ataggtgatt cacttcacat tcgccacctt cccaaaggca
      361 attggcaatt caaagaaccc gatgatgtaa cgcttgtgac agttcattct ttaaaagtgg
      421 aaactccagt tgagccaaca gctgctgtaa cccctgagga gcatacaact cctgaaccag
      481 aaaaataatc tatagtaatg aaactgatca ttggattggg aaatcctaca agagaatttg
      541 agcataaccg tcacaatttg ggtttcatct gtttagaccg ttgggcagcc gataagggaa
      601 gaaaatttgc caaaggcgag atcttcgatt atatttttat gcatagagct gttttgatta
      661 agccaagaac ctatatgaat agttccgggg aagcagttca aaaggcaatg gcatgttgga
      721 aaataacaga ttctatggtt atttatgatg atatagaatt accaacagct caaatccgca
      781 ttcgtcaagg gggaggagat ggaggccata atggcatcaa atctctgctg acagttatcc
      841 ccccagaaga attaaagaga attcggattg gaattggcag aaacaaagaa gttgaaccgc
      901 gagattatgt gttaagtgat attttacctg aagaatggga tttgctgaat ccaacacttg
      961 atgttgttgg caaatttatt gatatgtata ttaaatatga tttcaacgca gtgttgaatg
     1021 aatatagtat ctggaaaaaa tcctattccg gagcagacag ttctggaatc attagtccaa
     1081 aggaggaaag ttaatgataa aggattacga actaatgctg atcttaaatc ctaatcaaag
     1141 tgcagatgaa gccaatgcta tgaatgatag tttagtggct ttagttacag aaaatggagg
     1201 agagatcacc aaaattgacc cttggggaaa gcgtatgcta gcttatccga taaacaaatt
     1261 taacgagggt tattactatg tggattattt taagctggac agcttattag ttaaaaaact
     1321 aagcgcttcg ttcaaaatca atgaaaacat cattcgtcac caatttgtgg tgaaaaattg
     1381 aggagaatat aatggcagat ttaaaacttc cgcgtttaaa caaatgtatt ttcagtggaa
     1441 gaatcggtaa cgatatcgaa ctgaaatata cgccaaaagg aactcccgta gtacgttttg
     1501 ttgtcgcttg tgatcgtcgt tataaagatg atagcgaaca atggcaaaca gttaccagtt
     1561 ggatcgaatg cgtagcttgg aataaatggg ctgaatttat tgccaatcaa gttcataagg
     1621 gcagccctgt gattgttgaa ggcagaatcg aaacccgcaa ttggacagat caaaataatc
     1681 aaaaccgtaa aacaaccgaa gttatagtag aagttattca taatcttgaa tggaaacctc
     1741 gtgaaggaga ggcctcagca gtttctgaag atgttccttt acctgcagaa gaaacagccc
     1801 ctcataatgc tactaatact acaactgacg atgttccctt ttaagagaga ggaaaaaaat
     1861 gaacgataga aagaaaaaat actctcggaa gaaatactgt aaattctgcg ctaataaaga
     1921 tattcagatc gattataaaa atatagatat gctgaaacag tatatttccg aagtgggaaa
     1981 aattgaaccg gctcgtttaa ctggaacctg tgcaaagcat caacgtcagc ttgccagaga
     2041 aataaagcgt gccagacaaa tggctttaat tccttatgta attgagtaga tgcaaatctt
     2101 tcctcttata tttggagcta tatcactatt aagccctttt gtagctttaa tagtgtatat
     2161 gatgtatatt ggcaagacgg ctgaagtgca tttaactaaa ccgttacaag cctttttagt
     2221 gtttattccg gttccggtat tgatattatt attcgatcct caaactagtt ctcttttgct
     2281 tggtttggat gccatttttg ctgtaggatt gccggttttg atctttttgc tcactttaaa
     2341 gcataatcat attctcagta atgcgttttt ggctgcttat ttagttatag ctgcctgggg
     2401 aatattgagg tattatcttt ttcaaacata tcaaaatcag ctcttcgaac agggcttgca
     2461 aatggtgaaa gatagattgc cggctttgat ggataaatct ttaacggatc aaaccctgcc
     2521 aatttggaaa gcaataattc cagcgatttg ggttgtttca caatccatag cttggttgat
     2581 tggatttctc ttttttgaaa aaaatttgca gataccgaac aaaataacca atttgaggtt
     2641 tccggtttat tataacatac tgattatggc cgtgttacct ctttatttct ttgatcagac
     2701 aaaggttttg ttttacaatt tgctcattac tttatgtgtg attcctttca ttcagggagc
     2761 gtctttagtt tggcagcgtt tgagtttaat atttaccaac cggtttatca gtggtctttt
     2821 tatgataatc attgttctct atgccaatat tttgcttgtc cttataggat ttgcaaatat
     2881 gtggtttagt aaaagaaatt taactattga aggagatgca gcatgaaagt tattatgctg
     2941 aaaacgatag aaaaagttgg aaaacaaggt gaagttgtaa atgtaaaacg cggctttgcc
     3001 agaaattatt tagtgccgaa aaagttagct atatacgcaa ctccagccaa tctaaaaaat
     3061 cttgctaaca tccaaagtaa attagcggat gaagagcaga aattgatggc tgaattaaaa
     3121 aaccttgccg aaaaaataga tactactaat ttggttttta agcgcaaggt tgatgaacaa
     3181 ggtagtatgt ttggttccgt ttctgaacta gatattatca atggtttaca ggagctggga
     3241 attcagatta ataaaagtgc tttgtctatg gaaaagcatt ataaagaatt gggcgagttt
     3301 aatttgccgg tgcgtttgca taaggatata atttgctctt taaatattaa aattgagccg
     3361 gaacaataat aaattgtgga ggttttgatg tccgatctag gatataagat caaacaaata
     3421 ttaagtttga tcgtatttgt cgcagttcta tccttactag gaatgatcag tggccgaccc
     3481 ataatgatcc ttgcttatgg tgtatttttt cttgtggtgg tggcaattat gttttacctc
     3541 accaataaaa gacaacgcca ttttgagaaa gttaaagaat catcccctgt ttttaaaaag
     3601 gtctttggaa taatactggt gctgctctct ttaattactc caatggtaat tattttcaag
     3661 acaaacttag taaatttacc tgaatcctta cccataggca cagctattgc aatcgtcgga
     3721 gccgtttcta tagtttttat agctttaact ctggctgctg tatattttat taatatgcgt
     3781 ggcaaacaag taagtaacag agttatcggt tatatcctct atgtgatagc ggctatgatt
     3841 ccaggtttat tgatgagtag aattgaaaaa acaactattg gcattggctc tgtatattat
     3901 gttgctctga tcgttctaat attagcctat agtggttttg gattaatcac caatcgggaa
     3961 taaacgagct caaacattca ttttactctt caccggaatg cctggcaacc ggtgaagagc
     4021 tttattttgc tatgagtttc agttctatct caaaactttc aggacagctt gtttatcaat
     4081 taggtggcga taagtataaa aatttcatcc acatttacct ttcctggaag aaagttgtgg
     4141 gcgaactttt agcctcaaaa tcgtatccta taaagattga aaataaggtt ctttttgtaa
     4201 ccgtacacaa taatacctgg atgcaagaac tgattctgat taaggatgac atcatatcta
     4261 aatataaaaa tatctatgag gaagagattg ctgatattgt ctttcttatt agcagtccga
     4321 aaggtaaatg caaaagacgc aaata
//
LOCUS       DAORHB010000122         4328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_21767_length_4328_cov_3.950620, whole genome shotgun sequence.
ACCESSION   DAORHB010000122 DAORHB010000000
VERSION     DAORHB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21767_length_4328_cov_3.950620"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKM80_04395"
     CDS             <1..630
                     /locus_tag="PKM80_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HNV93182.1"
                     /translation="RYPQGAEKQLIYAATKRKVPAGGLPMAVGVVVQNVATAFAIYEA
                     IRYQKPLVQRVITVTGSPVVSPKNIMARIGTPLSELIEFCGGTSEEVGKVICGGPMMG
                     FALPSLEATIGKGSGGLVLLNKKAASLNEEGNCLRCARCVDVCPMNLVPCMIVSAIKY
                     NNLDLAVKSGLNDCIKCGACAYVCPAQIRLVQYIDTGKIRYAETKQAKK"
     gene            640..1743
                     /locus_tag="PKM80_04400"
     CDS             640..1743
                     /locus_tag="PKM80_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNV93183.1"
                     /translation="MNDKYIVSPAPHFHDKTTTKNVMWNVVLALTPALVFAVYYWGFK
                     SLLLTLLGAVSAMVTEALIQKFRKVPITISDGSAFLTGMLLAYNINAGAPWWIPVLGA
                     AFAIAVGKQVFGGLGNNPVNPALLGRAFLLASWPTIMTSGWVKPKSGSLSGITDLDAI
                     ALNLQSLSPKAYDLVTGATPLKVAQTLRDSSFVSSIITNTDQGVDLANRIFTNLTDLS
                     AIKNLFWGNIGGCIGEVSVFALLLGAAYLLYKNIIEWRIPLYYIGTVFVLSFFLAPLK
                     GSGFSPLLPFFHIFSGGLILGAFFMATDYTTTPITKNGRIIFAIGCGILTMVIRLYGG
                     YPEGVSYSILFMNVLTPLIDKLTMPKAFGWVKK"
     gene            1740..2330
                     /locus_tag="PKM80_04405"
     CDS             1740..2330
                     /locus_tag="PKM80_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNV93184.1"
                     /translation="MKIYLQLGLILLAFCLVATALLAYVNTITKPQIDKIKTLEAEET
                     RRTLIPDSDFEEITSDITYYIAKDNKTKEVKGYVFTAEKNGYNGPVKTMVAVDNNFQI
                     IAIKVTDQSETPGLGSNSTQPKFSEQFKGKTSEQLIVDKDGGVPPNCIKAITGATITT
                     RAVVNSVKESIELLKAKVQENSTSSEETKVKEEVNK"
     gene            2327..2917
                     /locus_tag="PKM80_04410"
     CDS             2327..2917
                     /locus_tag="PKM80_04410"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNV93185.1"
                     /translation="MSFIKELTKGIIKENPTFVIVLGMCPTLATSTSVNNAIGMGLAA
                     TFVLICSNIFISLIKNITPDQIRIPVYVIVIAAFVSIVDMSMAAYLPALHKSLGLFIP
                     LIVVNCIIMGRAEAFANKNNVVNSIADGIGMGLGFTLSLSVVATIREILGNGTWLNMR
                     VMPKTYDPMLITLLAPGAFITLGFLMALMNMLKEKQ"
     gene            2917..3519
                     /locus_tag="PKM80_04415"
     CDS             2917..3519
                     /locus_tag="PKM80_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HNV93186.1"
                     /translation="MGFLGQLFTMAISAILIQNFVLSRFLGLCPYLGVSKKMDSAFGM
                     GMAVIFVMTMASAFCYLIYQYLLVPFQLEYLQTLAFILTIAALVQFVEMVIKKNSPAL
                     YKSLGVYLPLITTNCAVLGVAILNITPLADGSRYSFIWAVLNGLFSGIGFTFVLLVMA
                     GIRERLEMVELPKSMRGMPSGLILAGTMALAFYGFMGMKI"
     gene            3539..>4328
                     /locus_tag="PKM80_04420"
     CDS             3539..>4328
                     /locus_tag="PKM80_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNV93187.1"
                     /translation="MLSIIVVPLAAGGISTILLPVAIMGSLGLIFGLVLAFASKVFAV
                     NIDERIEQIIEVLPGANCGACGQPGCAGYATAIVTEDKELNLCAPGGAAVVGKIAKIM
                     GKEASAQQRKIAYIHCSSGGKNNTKWKYDYQGIESCLSAVNIADGPNLCSWGCVGFND
                     CVAACKFDAISVDENGLRIIDRAKCTGCGACVNACPRRLIMLIPESRNVFITCSSKDK
                     NPLPKQNCGTDKPCIGCGLCAKKCPAQAITVENNIARIDYSKCTD"
BASE COUNT         1247 a          750 c          933 g         1398 t
ORIGIN      
        1 cgttatccac aaggtgctga aaaacagcta atttatgctg ccacaaaaag aaaagttcct
       61 gcaggtggat tgccaatggc tgtgggagta gtagttcaaa atgtggcaac cgcttttgct
      121 atatacgaag ctattcgtta ccaaaaacct ctggtgcaaa gagttataac cgtaactggt
      181 tctccggtgg taagccctaa aaatattatg gctcgaatag gaactccttt gtctgaattg
      241 attgaattct gcggaggaac tagcgaagaa gttggaaaag ttatttgtgg aggtccgatg
      301 atgggttttg ccttaccttc tttggaagct acaattggaa agggcagtgg tggattagtt
      361 ctattaaata agaaagcagc cagtttaaat gaagagggaa attgtttgcg ttgtgctcgc
      421 tgtgtagatg tttgtccaat gaacttagtt ccctgtatga ttgtatcagc tataaaatat
      481 aataatttgg atttagctgt gaaatctgga ttgaatgact gtattaaatg tggtgcctgc
      541 gcttatgtgt gccctgctca gattcgttta gttcaatata ttgatactgg caaaattcgt
      601 tatgctgaaa ctaagcaagc caaaaagtag gagaatgaaa tgaacgataa atatattgta
      661 tctccagctc cccactttca tgataaaact acaaccaaaa atgtgatgtg gaatgtagtg
      721 ttggctttaa cgccagctct tgttttcgct gtttattatt ggggttttaa atctctattg
      781 cttactcttt taggtgctgt atctgcaatg gtaactgaag cgctaattca gaaatttagg
      841 aaagttccaa tcacgatttc ggatggaagt gcttttttaa ctggtatgct acttgcctat
      901 aatataaatg caggagcccc ttggtggatt cctgttttgg gagcagcttt tgctatagca
      961 gttggaaaac aggtttttgg cggtttaggt aataatcctg ttaatccagc tcttttaggc
     1021 cgcgcatttt tacttgcttc ctggcctaca attatgactt ctggatgggt aaaacccaaa
     1081 agtggatctt taagtggaat cacagatctg gatgctattg ccctgaacct acaaagttta
     1141 tctcccaaag cttatgattt ggtaacaggt gccactcctt taaaagtggc tcaaacctta
     1201 agagatagta gctttgtttc ttctataatt acaaatacag accaaggtgt agatttagcg
     1261 aatcgaattt ttactaacct aactgatttg tctgcaatca aaaatctctt ctggggaaat
     1321 ataggtggtt gtattggtga agtgagtgtt tttgccttgc ttttaggtgc tgcctattta
     1381 ctttacaaaa acatcatcga atggcggata cccttatatt acattggaac ggtgtttgtt
     1441 ctctctttct ttttggctcc gctgaaaggt agtggtttta gtcctctgct gccatttttc
     1501 catatctttt ctggtggctt aatcttaggc gcttttttta tggcaacgga ttatacaact
     1561 acgcctataa ctaaaaatgg aagaataata tttgctatag gttgcggaat attaactatg
     1621 gttattcgtt tgtatggtgg ttatccggaa ggggtttcct acagcattct atttatgaat
     1681 gtgttaaccc ctctaatcga taaactaact atgcctaaag cttttggatg ggtgaaaaaa
     1741 tgaaaatata tttacaactt ggcttaatct tacttgcctt ctgtttagta gcaactgcac
     1801 ttttggctta tgtgaataca attactaaac ctcaaattga caaaattaag acattagaag
     1861 cggaagaaac ccgtagaaca cttattccag attccgattt tgaggaaatt actagtgata
     1921 ttacttacta catagccaaa gataataaaa ctaaagaagt gaagggctat gtttttaccg
     1981 ctgaaaaaaa tggctataac ggtccggtaa aaactatggt tgcagtagat aataacttcc
     2041 aaattatcgc tataaaagtt actgatcaat ctgaaactcc aggcttggga tcaaattcta
     2101 cccagcccaa attctcagag cagtttaaag gtaaaaccag tgaacaacta atagtagaca
     2161 aagatggagg cgtgccccct aattgtataa aagccataac tggcgctaca ataacaacta
     2221 gggcagttgt aaactctgta aaagaatcta ttgaattgct aaaagctaag gttcaggaaa
     2281 atagcacttc gtctgaagaa acaaaggtaa aagaagaggt gaataaatga gctttattaa
     2341 agaacttacc aaaggtataa taaaagaaaa tcccaccttc gtgattgtct taggtatgtg
     2401 tcctactttg gccacctcta cttctgtaaa taatgcaatt ggaatgggtt tggcggcaac
     2461 atttgtgtta atttgttcca atatctttat ttcgctaatc aagaatataa ccccagacca
     2521 aatacgaatt cctgtttatg taattgtgat agcggctttt gtatctatcg tagatatgag
     2581 tatggcggca tatttgcccg cactgcataa atccttggga ttgtttattc ccttgattgt
     2641 ggttaattgc ataattatgg gtagagcaga ggcatttgcc aataaaaaca atgtagtcaa
     2701 ttccattgca gacggcatag gtatgggact gggttttact ctttccttat ctgttgtggc
     2761 aactatccgt gagattttgg gtaacggaac ttggctaaat atgagagtta tgcccaagac
     2821 ctacgatcca atgttaatca cattgctggc acctggtgct tttattactc tcggattttt
     2881 gatggcattg atgaatatgc tgaaggagaa acaataatgg gctttcttgg acaacttttc
     2941 acaatggcaa tttctgccat tctaatccag aactttgtgc tttcccgttt tttaggtctt
     3001 tgcccctatt tgggtgtttc caaaaaaatg gattccgctt tcggaatggg tatggctgtt
     3061 atctttgtga tgaccatggc ttctgccttc tgctatctaa tatatcaata tctgctggtt
     3121 cctttccagc tggaatattt gcagaccctg gctttcattt taacgattgc agctttggtg
     3181 caatttgtgg aaatggttat aaagaagaat tctccagccc tttataaatc cttgggtgta
     3241 tatctaccac tcattactac taactgtgcc gttttgggcg ttgccatact taatattact
     3301 cctctagcag atggctctcg ttacagtttc atttgggctg tgttaaacgg attattttct
     3361 ggtattggct tcacttttgt gcttttggta atggctggaa tcagagaacg attggaaatg
     3421 gtagaattgc caaaaagtat gcgtggaatg ccaagcggat tgattcttgc cggaacaatg
     3481 gctttagcgt tttacggatt tatgggtatg aagatttaat taagggaatg gtgaagaaat
     3541 gttaagtata atcgtggtac ctttagctgc cggtggaatt tctacaatat tacttcccgt
     3601 tgctattatg ggttccttgg gattgatttt tgggcttgtt cttgccttcg cttccaaggt
     3661 ctttgctgtt aatatagatg aacggattga acaaataatt gaagtattac ctggtgcaaa
     3721 ttgtggtgcc tgtggtcaac caggatgcgc aggatatgcc actgctattg taactgaaga
     3781 taaagaattg aatttatgcg ctcccggagg agccgctgtc gtagggaaaa tagctaaaat
     3841 tatgggaaaa gaagcaagtg cccaacaacg aaaaattgcc tatatccatt gcagttccgg
     3901 tggaaaaaac aatactaaat ggaaatatga ctatcaagga attgaaagct gcctttctgc
     3961 cgtaaatatt gccgatggac caaatctttg ctcctggggc tgcgttggtt tcaatgattg
     4021 tgtagctgcc tgtaaatttg atgcgatcag tgttgatgaa aatgggttgc gaattattga
     4081 tagagcaaaa tgtaccggtt gcggagcttg cgttaatgct tgcccgcgta gattgataat
     4141 gctgattcca gaaagtagga atgtgttcat tacttgcagt tccaaagata aaaacccgct
     4201 gcctaaacag aattgcggaa cagataaacc ttgcattggc tgtggtttat gcgccaagaa
     4261 gtgtcccgct caggcaatta ccgttgaaaa taatatagca agaattgatt atagcaaatg
     4321 taccgatt
//
LOCUS       DAORHB010000123         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_22438_length_4242_cov_5.380702, whole genome shotgun sequence.
ACCESSION   DAORHB010000123 DAORHB010000000
VERSION     DAORHB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22438_length_4242_cov_5.380702"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..2567
                     /locus_tag="PKM80_04425"
     CDS             258..2567
                     /locus_tag="PKM80_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNV93188.1"
                     /translation="MEANSKAPAPFVLPVIFWIIGIILAKIIQINILFLLILASGLLI
                     LGVFCKKIRLILLIILFLVLGMLRFSIVKKESSPLALILQENEHLQQEICFTVTNVFS
                     VEEMRYAVELDSLAGFPLQDNIILSCSEKILPGKSYCLLAEIYPLVRDPILDIYPNRY
                     SAIGYQLGKAKSIDAKDVRDYLGRLRYKLLSSLEIKLGDQADWAKALLFGESGLKQEY
                     INQLTEAGIIHLVVVSGMHIWLIYMIIVSFFRIFFRRELAEFLFLPSILLYASLNNWA
                     PSVTRSIIMISIVILARWLQSPVSGAQTMALSIFIITLLSPMQLFNIGLQLSYISILV
                     IFYGLPHFRLFAQNKILNNSVYKMLENIKEGILLSTLISIAITPFTLYYFGTASLNGI
                     IGNIIGIPLMSIMLPLSMLVLISPQGWYITKLFVLCYKALNSLWISWIQFCHTLPFSL
                     SANYLSLYQALALAIVILWCFLLIRSKYKTALKTLIPASLLVMGFMLIPNINKQDASI
                     YVFNCGMGDCTLIRFQDGKTMLIDTGGGKNFGTPEAFVNEVDYQQESWLSKNLLPWLG
                     KKGINRIDYLILTHLHNDHCGGLITLLNHKKIGHIFVSDESVEQDFWNYAQKQKYFGS
                     AKIHTINDTCSFFIDKARLKFLHPDKNYLPHNENNASLVCRLDTKKESILFTGDIESE
                     AEFYLVDNYAKELKANYLKIPHHGSRSSSSTEFLEAVSPQEVWITAPRKNIYRFPHPD
                     TMQRIQKQQAKILLTGNGTIRKIGIPAKE"
     gene            3034..3537
                     /locus_tag="PKM80_04430"
     CDS             3034..3537
                     /locus_tag="PKM80_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93189.1"
                     /translation="MPEYWMHLLVKANITLDKLDTYLRDIWLECCGHLSAFTIEDRTY
                     TFTPFGEYEEKSMRIKLNEVLSPGMKFYYEYDFGTTTHLTLKVISEIELNAKGKFIRI
                     MARNDPPERNCVVCGKPATQVCVHCIREGMGWLCDECAPKHKCGEDMLLPVVNSPRVG
                     KCGYTGD"
     gene            complement(3712..>4242)
                     /locus_tag="PKM80_04435"
     CDS             complement(3712..>4242)
                     /locus_tag="PKM80_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV93190.1"
                     /translation="SIVGESGKGKTTVLNLIVRLISPQKGRIKFNGIDLNEYDTNYLR
                     SKIGYLTQNNIMYNIPLIDNVRYGVPDKSIEDVVRALSQVNMQRYTSSNYLFKTMGEG
                     GELLSGGEKQRVAIARLVLCNPDVILLDEPIANLDIDNAKCVMDLIFHLFSDKTIIIT
                     SHQPLAIAYSTRSILI"
BASE COUNT         1361 a          714 c          805 g         1362 t
ORIGIN      
        1 agtgcgggag ttcagagtgc gggagttcaa agttcaaagt gcgggagttc aaagttcaaa
       61 gtgcaggagt tcaaagttat agatcagtaa actaaatagc aataataaag agagctgata
      121 attggatatg aaatggaata aaaaacctga ttatctgtgc caataggttc tatccgtgaa
      181 atctatattt taaaactaaa tctgtgttat ctgtgaaatc tgtgagagaa gagaacgaat
      241 attaaaatgg agaaaagatg gaggcaaata gcaaagcccc tgctcctttt gttttgcctg
      301 ttattttttg gataatcggc ataatccttg ccaaaattat ccagattaat atcctctttc
      361 ttctcatttt agccagcgga ctattaattt taggggtttt ttgtaagaaa atacgcttaa
      421 tcctgctgat cattctattt ttagttttag gaatgctgcg tttttctatt gtaaaaaaag
      481 aaagctctcc gctcgccttg attctacaag aaaatgaaca tcttcaacag gaaatttgtt
      541 ttactgtaac aaatgttttc tctgtggaag aaatgcgcta tgctgttgag cttgattccc
      601 ttgccggttt ccctttgcaa gataatatta ttctctcttg tagtgaaaaa attcttccag
      661 gcaagtctta ctgtttgctg gcagaaattt atcctcttgt gcgtgatccg attttggata
      721 tttatcccaa tcgttattcc gctattggtt atcagcttgg caaagctaaa tctattgatg
      781 ctaaagatgt tagagattat cttggtagat tgcgatataa actgctaagt tctttagaga
      841 ttaaattggg tgatcaagcg gattgggcaa aagcgttact ctttggagaa tcgggtttga
      901 aacaggaata tataaaccag ctaacggaag ctgggataat tcacttggta gtagttagcg
      961 gaatgcatat ctggttgata tatatgataa tagtatcctt tttcaggata ttttttaggc
     1021 gtgagctggc agaatttctt ttcctgccgt caattttgtt atatgcaagt ttaaacaatt
     1081 gggctccttc cgtaactcgt tccataataa tgatatctat tgttattttg gcacgttggt
     1141 tgcaaagccc tgtttccgga gctcaaacaa tggctctatc tatatttatc atcactttgc
     1201 tcagccctat gcaattattc aatattggac ttcagctgtc ctatatatcg atattagtta
     1261 ttttttacgg tttaccccat tttcgactgt tcgcacagaa taaaatattg aacaattctg
     1321 tttataaaat gctggagaat atcaaagaag ggatattact ttcaactctg atatcaatag
     1381 ccatcactcc cttcactctc tattattttg gcacagcctc tctaaatggt ataattggca
     1441 acataatagg cattcctctt atgagtatta tgctacctct ttctatgtta gtgttaatct
     1501 ctccacaagg atggtatata accaagctat ttgtcctttg ttataaagcc cttaattcac
     1561 tttggataag ctggatccaa ttttgccata ctttaccctt ctctttatct gccaattact
     1621 tatcgctata tcaggctctg gctttggcaa ttgtaatcct gtggtgtttt ttgcttattc
     1681 gcagtaaata taaaacagct cttaaaacat taattcctgc ttctttactg gtgatgggct
     1741 ttatgctgat tcccaatatt aataaacaag atgcaagtat ttatgttttt aattgcggaa
     1801 tgggtgattg cacgctaatc cgttttcaag atggtaaaac tatgctgatt gatacaggtg
     1861 gaggaaaaaa ctttggaact cctgaagcat ttgtaaatga ggtagattac caacaggaaa
     1921 gctggctaag caaaaatctg ctaccttggc tggggaaaaa aggcattaat agaattgact
     1981 atcttattct cacgcattta cataacgatc actgtggggg gttgataaca ttgctgaatc
     2041 ataaaaaaat tgggcatatt tttgttagcg atgaaagcgt ggaacaagat ttctggaact
     2101 atgcccaaaa gcaaaaatac tttggctccg ctaaaattca tacgattaac gatacctgct
     2161 ctttctttat agacaaggca agattgaaat ttctgcatcc cgataaaaat tatctacctc
     2221 acaatgaaaa taatgcttcg ctggtttgcc gtctggatac aaaaaaggaa agcatccttt
     2281 tcactggaga tattgaaagc gaagcagaat tttatttggt tgataactat gctaaagaac
     2341 tgaaagcaaa ctatttaaaa atccctcatc acggttcaag aagttcaagc tccactgaat
     2401 tcttagaagc tgtttctcct caagaagtat ggataactgc tcctcgcaaa aatatttatc
     2461 gttttccgca tccggatact atgcaacgca tacagaaaca acaggcgaag attctgttaa
     2521 ccggtaatgg caccattaga aaaataggca tccctgcaaa agaatgatgt aactaagcta
     2581 taacatattg tcttatagct agaaaacaag agtatatgca gctggttgga aaaattgact
     2641 aaaaaatgaa cttgacacaa acttaaaaga tgaaaataag tagttaagaa tagaatgtaa
     2701 gtaacgaagg atattagatg ccctcaaaca agctgagaaa cgagttggaa ttttatacca
     2761 agatttggga ctcaactaat aagtaaaatt tggcaagagg gtaataagca atgatattat
     2821 tagtagtagc ataataaaaa aagaagacat aggaaaatgg caagaataca atctacaggt
     2881 aaatgttgtt tttgctcaag tacttttaat aagtcagcaa tgacaaggca cttgaaatcc
     2941 tgtaaggaaa gagaagcaaa atggaagtca tctttggaga acaatgaaaa acatcgtaca
     3001 aagttttttc atttgttggt ggagggaaga tacttgcctg aatactggat gcatctttta
     3061 gtaaaagcaa atataactct ggacaagtta gatacttatc taagggacat ttggttggaa
     3121 tgttgcggac acctaagtgc ctttaccatt gaggatagaa catacacctt tacaccgttt
     3181 ggggagtatg aagaaaaaag tatgagaatt aagttgaatg aggttctttc tccaggaatg
     3241 aaattttatt acgaatacga ttttggaact acaactcatc ttactttgaa ggttatatcc
     3301 gaaattgaac taaacgccaa aggcaaattt attcggatta tggcaagaaa tgatcctcct
     3361 gagagaaact gtgtagtatg tggaaaacct gctactcaag tttgtgttca ttgtattagg
     3421 gaaggtatgg gttggctttg tgatgaatgt gctcccaaac ataagtgtgg agaagatatg
     3481 ttgttaccgg tagttaattc tccccgggtt ggaaagtgtg gttatacagg cgattaatag
     3541 ttaacagccc ataaatatca caactaatta actataggcg taaacctctg gttggagtgc
     3601 tgtaaatgca tttttaaacc ggaggggtgt tcattttatt gagtctgtgt tctaataaaa
     3661 taatcggaaa accatagtat acaaaatgat tctcagcaaa tacaacaggc attatatcaa
     3721 tatggacctt gttgaataag ctatcgccaa tggttgatgg gaagtaataa taatcgtctt
     3781 atcgctaaaa aggtgaaaga ttaaatccat aacgcattta gcattatcaa tatcaagatt
     3841 ggctattggc tcatcaagaa gaatgacatc tggattacat aatactaatc ttgctatcgc
     3901 tactctttgt ttttcacctc cagataacaa ttcccctccc tctcccatag ttttgaacaa
     3961 gtaatttgaa cttgtgtagc gctgcatatt tacttgagac aatgctctga ctacatcctc
     4021 aatggacttg tctgggaccc cataacgaac attatctatc aagggtatat tatacataat
     4081 attattctga gtcaagtagc caatcttaga cctaaggtaa tttgtgtcat attcgtttag
     4141 gtctattccg ttaaatttta ttcttccctt ctgaggagat attaatctca caattaggtt
     4201 taacacagtt gttttacctt taccgctttc acctacaata ga
//
LOCUS       DAORHB010000124         4217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_22629_length_4217_cov_3.918789, whole genome shotgun sequence.
ACCESSION   DAORHB010000124 DAORHB010000000
VERSION     DAORHB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22629_length_4217_cov_3.918789"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3538
                     /locus_tag="PKM80_04440"
     CDS             <1..3538
                     /locus_tag="PKM80_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93191.1"
                     /translation="IAYCLIALDGTDFYSQAKGVTELQNIFSGYQNTEVMNRIVDKAA
                     SNKFDLAASLQAIDFLWLKINMNPMALENPTLVDSILKIYASAQYQLREDQNFDQIAH
                     ELYQNIITNYGKNSTWYATNKDKNISAQLAVIQNAYEKRALRLHNEFLAEPTNETRLL
                     AYYQHMDQYSSFMETHSENYPAWKAKTDKNKLLLSITLAERSNLPKHYLSAIKCLQDF
                     NTNYPQDEDFFLNEGLIYVYTNNIYNLMKDLYNENYNPEPGLPANLDELFALLSTNSM
                     RFINVLRSDAYKNPERESQAVTILLNLADIQYNRGKMPEAKELYLTALQNETLISNKT
                     KFDIYGKLALISEQEKNFAQAETYYRKALPFASTPTEREAIANNIRYQIQNSYEAAEQ
                     AGNYSFAASERLRLAAELDPKDTQRIQGYKMAAQETYIKAKEYQKAIDILLELAGTKT
                     DIDEIYYYYFRAAEIAEADTAMNNPEIAKNIKDSFIAKYPSSNQAFLLRIAMLQEIEK
                     DPAQKNAAAEAYLALHEEARKKTINTGDITPDALLLKASINYREAGNKEKEIEIYNQF
                     LQLYPNNENVIPFMQVIADDYLAKGDTLKFEQLAKQIYTKDKTKGDRYQWIATTKLNK
                     LMYDFDNAYKNKNYSEAFKYRDEYKRLEAAYKKEGLQFETADFSSAKNYEYFTSVENE
                     YKAIEKRKAFLKNFDAQLTAIEKGNLMTSSPAKLIKVNVNTTWQNHLIGGSYRRIPIF
                     KESVRAEVAKVNKVLSSAEKENIELENYRRLRALNLIARIYEKGVNVINTQIGVYVRT
                     SYEATGVRQQYKDILDSFINSVAADQSNDLLSLAYTTHLNIYNYYQMAGYTDYYTQQS
                     YAKLQQWNLLPDYKIDEYPLGSGWSQKMDGNNINLPAQNVTSPKGGRLGSIQIPAGKD
                     IILSHTVNAKLVPDFALIQLVYPYDVNIRMNGTDVEAGVVPIDTLDVNKPGTTTRYAY
                     LLPSSSWIEGQNAVELTVPNNTPEPQNLYLSLQVFTDRQKIAASIPPETVMLYTDPSW
                     RIVNVNQETGTETFSAATITGNFGISLDNIEGIEGEQAKPIWPVEEAPISSAVFEVDF
                     YLDTEFREGLINFVAPETATVYLNGELLASNLAMDYDPEPFRVYTTQVSIEPAKVTSG
                     KNTLRFVVQNNSNYRGFIATVKIIKAGKEEVR"
     gene            3535..3837
                     /locus_tag="PKM80_04445"
     CDS             3535..3837
                     /locus_tag="PKM80_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93192.1"
                     /translation="MKKSFLLLAAMLFLAYIGYAQTVTPQPTNQQQPKGFEGVGQYVE
                     KEELIIEVKSLIPPTGVFQDKSDTYQKSGEYKFSFSLEDIRKQSDILLNKWLKESK"
     gene            3834..>4217
                     /locus_tag="PKM80_04450"
     CDS             3834..>4217
                     /locus_tag="PKM80_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93193.1"
                     /translation="MKAKHIVLVIISLFICVGLFGQNAGEAAAPAAPASQGYSIVQVF
                     VDSGNWAYAILLVFIIGLVYAIVRWNQLYRKEKIDAGGFYLKLRNYIRNDQLEEAAKI
                     AESYKGTTMGFIFWSALMISKILEKV"
BASE COUNT         1420 a          761 c          826 g         1210 t
ORIGIN      
        1 tattgcctat tgtttgatcg ctctggatgg aactgatttc tattctcaag ctaagggagt
       61 aacggagctg caaaatatct ttagtggtta tcaaaataca gaagtaatga accgcattgt
      121 agataaggca gcctccaata aatttgatct tgctgcttct ttgcaagcaa ttgacttcct
      181 gtggcttaag atcaatatga atcctatggc tcttgaaaat cctaccttag tagattctat
      241 cctgaaaata tatgccagtg cgcaatatca gctacgcgaa gatcaaaatt ttgatcagat
      301 agctcatgag ctctaccaaa atataattac taactatggt aagaactcta cttggtatgc
      361 aacgaacaaa gataaaaata tctcagcgca gctggcagtg attcaaaatg cttatgagaa
      421 aagagcactg cgtctgcata acgaatttct tgcagaacca acaaatgaaa ctcgcttgct
      481 tgcctactat caacatatgg atcaatatag tagttttatg gaaacgcata gcgagaacta
      541 tccagcttgg aaagcaaaaa cagataagaa caaactatta ttatctataa ctcttgccga
      601 acgctctaat ttacccaagc actatctttc tgctattaaa tgcttgcagg actttaatac
      661 taattatcct caagatgagg atttcttcct aaacgaaggt ctgatttatg tgtatactaa
      721 taatatatat aatcttatga aagacctcta caatgaaaat tataatccag aacctggctt
      781 gcctgcaaat cttgatgaac tgtttgctct attaagtacc aattcaatgc gtttcattaa
      841 tgttttaaga agcgatgctt ataaaaaccc tgaacgcgaa agccaagcag ttactatctt
      901 attaaacttg gcagatattc aatataatag aggtaagatg ccagaagcaa aagagttgta
      961 tcttacggct ttgcaaaatg aaacgcttat ctccaataaa actaaatttg atatatacgg
     1021 caaactggca ttaatttctg aacaagagaa aaactttgct caagctgaaa catattaccg
     1081 taaagcttta ccttttgctt ctacccctac tgaaagagaa gctattgcaa acaatataag
     1141 ataccaaatt cagaatagtt atgaagctgc tgaacaagca ggtaactatt cttttgctgc
     1201 tagtgagcgg ttgcgtttag ctgcagaact tgatccaaaa gatactcagc gtattcaggg
     1261 atataaaatg gctgctcaag aaacatatat taaagccaaa gagtatcaga aagccataga
     1321 tattttattg gaattagcag gaacaaaaac tgatatagac gaaatttact attattactt
     1381 ccgcgctgca gaaatagctg aagcagatac ggctatgaat aatccggaaa tagctaaaaa
     1441 catcaaagac agctttatag ctaaatatcc cagtagcaat caggcatttt tattgcgaat
     1501 tgctatgctt caagagatag agaaagatcc tgcacaaaaa aatgcagcag ctgaggctta
     1561 tcttgcgttg catgaagaag ccagaaaaaa aaccatcaat actggtgata tcactccaga
     1621 tgccttattg ttaaaagcca gtattaacta tcgtgaagcc ggaaataagg aaaaggaaat
     1681 tgaaatctac aatcaatttc tacagctcta tccaaacaat gaaaatgtaa ttccctttat
     1741 gcaggttatt gctgatgact atctggccaa gggagatacg ctgaagtttg agcagttagc
     1801 aaaacaaata tatactaagg ataaaactaa aggcgatcgc tatcagtgga ttgccactac
     1861 taagttgaac aaattaatgt atgattttga taatgcctac aagaataaaa actattctga
     1921 agcatttaaa taccgcgatg aatacaaacg gctggaagct gcttacaaaa aagaagggct
     1981 tcaatttgaa actgcagatt ttagctccgc taagaactat gagtatttta catcagtgga
     2041 aaatgaatac aaggcaattg aaaagcggaa agcgttctta aagaacttcg atgctcaact
     2101 tactgctatt gagaaaggca acttaatgac ttcctcccct gctaagctaa tcaaggttaa
     2161 tgtaaatact acctggcaga accatttaat tggtggttcc tacaggcgta ttcccatatt
     2221 taaggaatcg gttagagcag aagtagccaa ggtgaacaag gttcttagtt cagccgagaa
     2281 agaaaatatc gagttagaaa actatcgtcg gctgcgtgct ttaaatctaa tagctcgtat
     2341 ttatgaaaaa ggtgtgaatg ttataaacac ccaaattggt gtttatgttc gcacttctta
     2401 tgaggcaacg ggtgtgcgtc agcaatataa agatatattg gattctttta ttaattctgt
     2461 tgctgctgat caaagcaatg atctgctcag tttggcatat acaactcact tgaacatata
     2521 taattattat cagatggctg gttatacaga ttattacact caacaaagct atgccaaatt
     2581 acagcaatgg aatctgctac ccgattataa gatagatgaa tatccgcttg gcagtggctg
     2641 gagccaaaaa atggatggta ataatattaa tttacccgct caaaatgtaa cttcacctaa
     2701 aggtggtcgt ttgggcagta ttcagattcc tgcagggaaa gatattattc tcagccatac
     2761 tgttaatgct aaattggtac ccgattttgc cctaatccaa ctagtttatc cgtatgatgt
     2821 aaatattaga atgaatggta cagatgtaga ggcaggggtt gttcctattg acactttgga
     2881 tgttaataaa ccgggaacta ctactcgtta tgcatattta ttgccttcca gttcttggat
     2941 tgaagggcaa aatgcagtgg aattgactgt ccctaataat actccagagc cccagaatct
     3001 gtatttatcg ttgcaagtat ttacagaccg ccagaaaata gctgcttcca tccctccgga
     3061 aacggttatg ctatatacag accccagctg gaggatagta aatgtgaatc aggaaactgg
     3121 aactgaaaca tttagtgcag ctacaataac cggtaatttt gggatcagtt tggataacat
     3181 tgaagggatt gagggtgaac aagctaaacc gatttggcct gtggaagaag cgccaattag
     3241 tagtgcagtt tttgaagtgg atttctatct ggatactgaa ttcagagaag gtctgatcaa
     3301 ttttgtggca cctgaaactg caactgtgta tcttaacgga gagttattag cttccaatct
     3361 tgctatggat tacgatccgg aaccgttccg cgtatatact actcaggttt caatcgaacc
     3421 cgccaaagta acttcaggga aaaataccct gcgttttgta gtgcagaaca attccaacta
     3481 tcgtggcttt atagctacag taaaaatcat caaagctgga aaggaggagg tacgatgaag
     3541 aaaagtttct tactactggc agcgatgctc tttttggcat acatcgggta tgcccaaacg
     3601 gttactccac aacctactaa tcaacagcaa ccgaaaggtt ttgaaggtgt tgggcagtat
     3661 gtagaaaaag aagaattaat aattgaagtg aaatccttaa ttcctcctac gggtgttttt
     3721 caggataaat ccgatacata tcagaaatca ggagaataca aattttcctt ttctttagaa
     3781 gatattcgaa aacaaagcga tatattacta aataaatggc tgaaggagtc aaaatgaaag
     3841 caaaacatat cgtcctagtg attatctccc tgtttatttg tgttggtcta tttggccaga
     3901 atgcaggaga agccgcagct ccggctgcac cagctagtca aggttacagt atagttcaag
     3961 tatttgttga tagcggcaat tgggcttacg caattctgct tgtctttatt attggtcttg
     4021 tttatgccat tgtccgttgg aatcaactct accgtaaaga aaaaatcgat gcaggtggat
     4081 tctacttgaa gcttcgtaat tatattagaa atgaccagct cgaggaagca gccaaaattg
     4141 ctgaaagcta taagggaact acgatgggct ttatcttctg gagcgctttg atgatttcaa
     4201 agattctaga aaaagtg
//
LOCUS       DAORHB010000125         4197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_22780_length_4197_cov_3.614196, whole genome shotgun sequence.
ACCESSION   DAORHB010000125 DAORHB010000000
VERSION     DAORHB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22780_length_4197_cov_3.614196"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKM80_04455"
     CDS             complement(<1..581)
                     /locus_tag="PKM80_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV93194.1"
                     /translation="MTKEEMIQDINSMQSLKDALLNEIHNVIIGQDRVLTDVLIALFA
                     NGHILLEGVPGLAKTLLISSLAKALSLSFSRIQFTPDLMPSDITGTDILVNDPITEQK
                     RFEYIKGPIFANIILADEINRTPPKTQAALLQAMQEYAVTSGTVTRPLSKPFLVMATQ
                     NPIEQEGTYPLPEAQLDRFMFFINIDYPTMEEEL"
     gene            complement(574..1578)
                     /locus_tag="PKM80_04460"
     CDS             complement(574..1578)
                     /locus_tag="PKM80_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNV93195.1"
                     /translation="MTHKNRNTLIIGLIVGIILLAAWLYYTPISEIRTQIAKVNYNWV
                     IFASLAYLFAYFLRSLRWRLLLPAQANPGICRTWLYSMAGNLLNYLIPIRAGDFARGW
                     FIKKNYDIPYAKALPSVFVDKVFDTLAILFIIIVLPFTTIELSTAMIILLCLLMLVFI
                     VAFSILVASVWQKEWVIKMLTFIFSWLPQKAKDRINPAIEMFIEELNIFEHQPLKLIL
                     ALLLTAFGILMDGTYFYLLFRAFGILYPFAITLFGYTLINLSYAIPQPPAQLGSNEWM
                     MIIIFSIGFGLTKSTASAIMAFGHILTAGLMSLWGIIAFAVLGPELFISVFKGEKIND
                     "
     gene            complement(1578..2414)
                     /locus_tag="PKM80_04465"
     CDS             complement(1578..2414)
                     /locus_tag="PKM80_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV93196.1"
                     /translation="MAVPIINDWAKYFDNPDEGLGSSYERVILNQLLDSICEVYHIQS
                     ALETPCFGFTGVSGINLVNLAKQGIKVSLEDHDLERITKINELWQELRLHIDIKYNPE
                     YVKLDYPDKHFDLGFNFSALWFTQNIRSFLAEFCRVCKKAILICVPNRDGIGFKMQMK
                     DYSPKKYPYLHPAHLDLATIKYLMKQNKWLLQKEGYIDCPPWPDIGMKKELFFKKLVG
                     KQEIQEKNREPLSILSYYQEENSDFISRMLSYGFVEKTAPPSFKKHWAHHYYLLFTPD
                     NG"
     gene            complement(2414..3397)
                     /locus_tag="PKM80_04470"
     CDS             complement(2414..3397)
                     /locus_tag="PKM80_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV93197.1"
                     /translation="MDRLLITGITGFIGRNVLKELMNTCPDYSITALVRPQTHPNRYS
                     EFGKDLRIIEIDLADTASLKEFLFTNEFDTILHIGALRGGRKFPKETYLRSNVYSTGQ
                     MVEYCLAKEARLIYCSSVGVFGAIPQELPAGPETEKNPDNYYHYTKIESERIVNRAIL
                     NGLKASIIRPSIAYGEGDFGFPYQLVKLVDKYMFPLINRPIWIHLCHIDALVKAFVYL
                     ATNEWKSGLTLTIADREPVRLQALVDFISRQLHNKNYPGVLTFDRIFFAWGEKLARML
                     KNELWISRFELISKSWFYDVTAYYDIMEREGIKPHFTIPEFEITIKDYQKH"
     gene            complement(3390..4178)
                     /locus_tag="PKM80_04475"
     CDS             complement(3390..4178)
                     /locus_tag="PKM80_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HNV93198.1"
                     /translation="MVDIHTHLLPNIDDGSKSLDDSLSQISLMAEGGISRAYLTSHYF
                     RGHFQYTRSEYDDKFNLLVNEVIKAGIDIDLQPGFEIYLQPGILSDIKQHNLTMGKSN
                     YILLESDLNGLPSDFYNNIYPLLRAGYKPILAHAERYVSIMQKPSRAEDLIHRNIYIQ
                     TSAGSLLGLYGEKVKSTAWILVNKGWTHFIASDDHLRGKYYSYFEAKNKIEEVIDNHT
                     ANLLFYLYPSCIDNGDYIPYEYVEVHHSHHHQHHKHSLLKRIFG"
BASE COUNT         1397 a          754 c          735 g         1311 t
ORIGIN      
        1 agttcctctt ccatagtagg gtaatctata ttgatgaaga acataaaacg gtctaattgc
       61 gcttccggta agggataagt accttcctgt tcaattggat tttgcgttgc cataactaaa
      121 aaaggtttac ttaaagggcg ggtaacagtt ccactggtaa cagcatattc ttgcattgcc
      181 tgtaataaag cagcttgggt tttgggcgga gttctgttta tttcgtctgc caaaatgata
      241 ttggcaaaga taggtccttt aatatactca aagcgttttt gttcagttat cgggtcatta
      301 accaaaatat ctgttccggt gatatctgag ggcataagat caggggtaaa ttgaatgcgg
      361 ctgaaagaaa gggagagtgc ttttgctaaa gaagaaataa gtaaagtttt agcaagtccg
      421 ggaacccctt ccagtaaaat atgaccattg gcaaaaagag caatcaggac atctgtaaga
      481 actctatcct gcccaattat gacattgtga atttcattaa gtaatgcatc ttttaaactc
      541 tgcatcgagt ttatatcttg tatcatttct tctttagtca tttatttttt ctcctttaaa
      601 aacactaata aaaagctctg gaccaagaac agcaaaagca attatccccc ataaactcat
      661 taagccagct gttaaaatat gtccaaaagc cataattgct gaggcagtgg actttgtcaa
      721 tccaaatcca atgctgaaaa taataatcat catccattca ttactgccca gctgagccgg
      781 tggctgagga atagcataag atagatttat aagtgtgtaa ccaaacagag tgatagcgaa
      841 aggatataat atcccgaaag cacgaaaaag caaataaaaa tatgttccat ccattaaaat
      901 tccaaatgcc gttaagagca aagctaaaat aagcttcaag ggttgatgtt caaaaatgtt
      961 gagttcttca ataaacatct ctatagcagg attgattcta tcttttgcct tttgtggaag
     1021 ccaggaaaag ataaaagtga gcatttttat cacccattct ttctgccaga cagaagccac
     1081 taaaattgaa aaagctacaa taaacactaa catcaataga cataataaaa taatcattgc
     1141 tgtgcttagt tctatagtag taaaaggaag aacgattata ataaataaaa ttgctaaggt
     1201 atcaaaaacc ttgtccacaa aaacggatgg aagagcttta gcataaggaa tatcataatt
     1261 ctttttgata aaccaaccgc gggcaaaatc tcctgccctg ataggtatta aatagtttag
     1321 taagtttcca gccatagaat atagccaagt tctacaaatt ccagggtttg cttgagcggg
     1381 aagtaataat cgccagcgta aagaacgcaa aaaataagcg aaaagataag caagagaagc
     1441 aaaaataacc caattataat taactttggc aatctgagtt ctaatttcgc tgatcggagt
     1501 ataatataac caggcagcaa gtaatattat gcctacaatt agcccaatta tcaatgtatt
     1561 acggttttta tgtgtcatta accgttatcc ggagtgaaca gaagataata atgatgtgcc
     1621 cagtgttttt taaaactagg tggtgcagtt ttttccacaa aaccatagga aagcattctt
     1681 gatataaaat cactattttc ttcctgataa taggagagaa tagagagtgg ttctctgttt
     1741 ttttcctgta tttcctgctt tccgaccaat tttttaaaaa agagttcctt tttcattccg
     1801 atatcaggcc agggaggaca atcaatataa ccttcttttt gtagtaacca cttgttttgt
     1861 ttcatcagat atttgatagt agccaaatca agatgagcag gatgcaaata aggatatttt
     1921 ttgggggaat aatccttcat ctgcatttta aaacctattc catcacgatt gggaacgcag
     1981 atcagaattg cctttttaca tactctacag aattcagcaa ggaaacttct aatattttga
     2041 gtaaaccaaa gcgcagaaaa gttgaaaccc aaatcaaaat gtttatccgg ataatcaagt
     2101 tttacatatt cagggttata tttgatatca atatgcaggc gtaattcttg ccataattca
     2161 ttgatttttg taattctttc cagatcgtga tcttccaagg agacctttat tccctgttta
     2221 gctaaattta caaggtttat tccgcttacc ccagtaaaac caaaacaagg tgtttccaac
     2281 gcagattgaa tatgatatac ttcacaaata gaatctaata attggttcag gataactcgc
     2341 tcgtaggaag agcccaatcc ttcatcagga ttatcaaaat atttagccca atcgttgata
     2401 atgggaacgg ccatcagtgc ttctggtaat cctttattgt tatttcaaat tcaggaatag
     2461 taaaatgagg ttttatacct tctctttcca taatatcata atatgcggtt acatcgtaaa
     2521 accagctttt ggatataagc tcaaagcggc tgatccaaag ttcattttta agcattctgg
     2581 caagcttttc accccaagca aaaaatatcc tgtcaaaagt taaaacacca gggtagtttt
     2641 tattatgtaa ctgacgggaa ataaaatcta ccaaagcctg taaacgaact ggttcacgat
     2701 ctgcaattgt aagggttaaa ccacttttcc attcgttagt tgccaaatag acaaaagctt
     2761 ttaccaaggc atcaatgtga catagatgaa tccaaatcgg acggttaatt aatggaaaca
     2821 tatatttatc tacaagctta accaactgat aaggaaaacc aaaatcacct tctccataag
     2881 caatactggg tctaataata gatgctttca gtccatttaa tattgctcgg ttgactatgc
     2941 gttccgattc aattttagtg taatgatagt agttgtcagg atttttctcg gtttcaggtc
     3001 cagcaggtaa ttcttgaggg atagctccaa aaacacctac cgaggagcaa taaatcaatc
     3061 ttgcttcttt tgccagacag tattctacca tttgtcctgt actataaaca ttgcttcgta
     3121 aatatgtttc tttcgggaat tttcttcctc cgcgtaaagc tccaatatgt aagatagtat
     3181 caaattcatt ggtaaaaaga aattccttta aagaagctgt gtcagcaagg tctatttcaa
     3241 tgatcctaag atcctttcca aattcagaat atcggtttgg atgtgtttgg ggacgcacta
     3301 atgcagttat gctatagtca ggacaggtat tcattaattc ctttaaaaca tttctaccaa
     3361 tgaaaccagt gataccagtt ataagaagtc tatccaaaaa tcctctttag caaactatgt
     3421 ttatgatgtt ggtgatgatg agaatgatga acttcaacat attcataggg gatataatct
     3481 ccattgtcaa tacaggaagg atataaataa aaaagcagat tcgcagtatg attatcgatc
     3541 acttcctcga ttttattttt tgcttcaaaa taagaatagt attttccccg taaatgatca
     3601 tccgaagcaa taaaatgtgt ccaaccctta tttactaata tccaggcagt ggatttaact
     3661 ttttcaccat acagaccaag taagctccct gcactagttt gtatataaat gttacgatga
     3721 ataaggtctt cagcgcgtga gggcttttgc ataatactca catatcgttc tgcgtgtgcc
     3781 aaaataggtt tataaccagc tcgtaataag gggtagatgt tattgtaaaa atcactgggt
     3841 agaccattta agtcactttc caaaaggata taattgcttt tacccatagt tagattatgt
     3901 tgttttatat cagataggat gccgggttgc aaatatattt caaatccagg ttgcaaatcg
     3961 atatctatac cggcttttat aacttcatta actagcaggt tgaatttatc atcgtattca
     4021 cttcgcgtat attgaaaatg tcctctaaaa taatgagaag ttaagtatgc tctactaatt
     4081 cccccttctg ccatcaaaga tatctggctg agagaatcgt ccagtgattt tgaaccgtca
     4141 tcaatattgg gtaataaatg agtatgaata tctaccattg cttaaggtaa tctttcc
//
LOCUS       DAORHB010000126         4139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_23282_length_4139_cov_3.790891, whole genome shotgun sequence.
ACCESSION   DAORHB010000126 DAORHB010000000
VERSION     DAORHB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23282_length_4139_cov_3.790891"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKM80_04480"
     CDS             <1..345
                     /locus_tag="PKM80_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93199.1"
                     /translation="LLLGGTVAAAHFGFKAYKKISGTAKLAKSLPEFLNNVYGEMPEV
                     NVNNTLNSVRIRAFFSQEILDKHTDIDNTIREYIDDFYPEIAKCSVDITIMPKMEETE
                     ETATEEEPEEKE"
     gene            complement(942..2777)
                     /gene="glmS"
                     /locus_tag="PKM80_04485"
     CDS             complement(942..2777)
                     /gene="glmS"
                     /locus_tag="PKM80_04485"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNV93200.1"
                     /translation="MCGIVGYIGYRNALPIVVEAIKRLEYRGYDSSGCALIHKDELQV
                     YKRQGKIIELERCLPEPNKCSGNIAIAHTRWATHGEPNEVNAHPHLDCKGEIAIVHNG
                     IIENYKILKDRLLELGHTFISETDTEVIVHLIEQYLVSEPTLEDAVCEAMKRVEGTYG
                     LVVISIKEPDKLIAVRKGSPLIIGIGENEHFITSDVNAIIIHTKRVIYLQDGELCIVK
                     KDNFEITTLDKRSVKPEISVVDWDISAIEKGEYKHFMLKEIFEQPITIENAFRGRINQ
                     QLGTARLRGLHIPSFELKKVKQVHLVACGTSFYAGLIGKYIIEDLARIPVFAEYASEY
                     RYRNPIIPEDTLVFVISQSGETADTLAALREAKAKGARVLGITNVVGSTIARESDGGT
                     YIHAGSEIGVASTKAFTSQVTIMTLLAILLGRLNHLSPIQGMEYIKELENIPAKVEKI
                     LAQNEYIRNIAATIKDCTNALYLGRGINYPVALEGALKLKEISYIHSEGYPAAEMKHG
                     PIALIDENMPVIAIATKDPLYDKIYSNLQEVRARKGRLITIATEGDTELAKISENVIY
                     IPDTLTNLQPLLTVIPLQLLAYNVADLKNLDVDQPRNLAKSVTVE"
     gene            complement(2765..>4139)
                     /locus_tag="PKM80_04490"
     CDS             complement(2765..>4139)
                     /locus_tag="PKM80_04490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNV93201.1"
                     /translation="EKERKTLETLLVSSVGRTEIVMGKYLTIITLAMLNMIVNLFSIS
                     FSLQFMLANQSKEMSGVTLPINAMLILLIAMIPLATLFAALLVSISTFSRNIKEARSY
                     EQPLLMIAMLMGMVSFIPTIEISNLLAIVPVVNIALLFKAVLINEYTLSHILITIIST
                     LVLDLIAILITVKLFKTESVLFRTEEESGGIKVLKKKPKYALTPYNGIVYFTIALILL
                     YYLGGYWQTKDLYNGLIQTELIIIALPVFIVLKLLKLKPKEILRFKTPKPAGLILVPF
                     IAISASIIVSIMSQLINTIFPYPEKYTEALTQLFNMNEAPWKVFLAIALLPGICEELL
                     FRGFLIRFFEKYGLQWAVIISAILFAAFHLDPFRFVPVLLLGFLLGYLALRSGSVYTS
                     MLSHIINNGLAFILVTYSNSSWVKFLVSEGDNIHYWLFIPALVVFVISLYAFHKVTAQ
                     GEEICVE"
BASE COUNT         1379 a          763 c          668 g         1329 t
ORIGIN      
        1 cttttattag gtggaaccgt agctgctgct cattttggtt tcaaggcata caaaaaaatt
       61 agcggcactg ccaaacttgc caaatccctg cctgaatttt tgaataatgt ctatggcgaa
      121 atgccagaag ttaatgttaa caacactctc aattcggtaa gaatcagagc tttcttcagc
      181 caggaaattt tagataaaca taccgacatc gataatacta tccgtgaata tatcgatgat
      241 ttctatcccg aaattgccaa gtgctctgtg gatattacta ttatgcccaa aatggaagaa
      301 actgaagaaa ctgcaaccga agaagaacct gaagaaaaag aataagaaaa ctaactttta
      361 caaactataa acccggtttt ttagagaacc gggttttttg tgttgtgaat gccattaata
      421 aagaaatact ataaccaccg ctcctttttt aatttcaatt gtgcggatgc ttgttttaat
      481 gtatccttac ttccaaaact ataatcatca ttaactgatt tcacaatttt caagttgagg
      541 tttatgaagt aaaatatagc atttatggca attggtacag cgtattgtaa attcttgtta
      601 agaaaaagtg acctggattt tataatttaa ctccaagcta atgatgtttt attgtgcaac
      661 attcttaaca tataaagatt atcttaagct aaaccttact ttctcttgca agttaaccag
      721 aagtaactcc tcatccaccc aatcttcact catccttaac tatgggttga agaggagagg
      781 aggaggagag gagtatgggt tgttgaagtg tcctaaaaaa ggtggacttg aggcgtccta
      841 aataaattga ttctgtatct agcatcgaaa atactgaaaa gtagagataa tatcatacca
      901 gggatatagg atatttctgt ccggcaatat atcttcagat tttattctac cgtaacactt
      961 tttgccagat ttctcggttg atccacatcc aaatttttca ggtctgctac attgtaggca
     1021 agcaattgca gaggaataac ggttaaaagt ggttgtagat tagttaaagt atcgggtata
     1081 tagataacat tttccgatat tttagcaagc tctgtatctc cttcggtagc gatagttatc
     1141 aagcgtcctt tgcgggctcg aacttcttgt aaattagagt atattttatc atagagcgga
     1201 tcttttgtag caattgcgat cacaggcata ttttcatcta ttaaagctat aggaccatgt
     1261 ttcatttcag cagctggata tccttcagaa tggatatagg agatttcttt taattttaaa
     1321 gctccttcca aagcaacggg ataatttatt cctctaccca aataaagtgc attagtacaa
     1381 tccttaatag tagctgcaat atttctgata tattcgtttt gagcaaggat tttttctacc
     1441 ttggctggga tattttccaa ctccttaata tattccatcc cttggattgg agatagatga
     1501 ttcaatcttc ctaaaaggat tgctagcaag gtcataatag taacttgaga agtaaatgcc
     1561 ttggtactgg ctactccaat ttcacttccg gcatggatat aagtgccccc atcgctttct
     1621 ctggcaatgg tactaccaac aacatttgtt atgcctaaaa ctctggctcc tttagcttta
     1681 gcttctctta atgctgctaa agtatctgca gtttcccctg attggctaat tacaaatact
     1741 aaagtatctt ctggaataat tggattccga taacgatatt cagaagcata ttctgcaaaa
     1801 acaggaatac gggcaagatc ttcaattatg tatttaccga ttaatcctgc ataaaaagaa
     1861 gttccacagg caactaaatg aacttgttta acttttttca attcaaagct aggaatatgc
     1921 aatcccctta agcgtgcagt tccaagttgt tgatttatcc tacctcgaaa agcattttct
     1981 atagtgatgg gttgttcgaa aatctctttc aacataaagt gtttatattc gcctttttct
     2041 atagcagaaa tatcccaatc cactactgaa atttctggtt ttacgctgcg tttatccaaa
     2101 gtagtaatct caaaattatc tttttttaca atacataatt caccatcttg taaatagata
     2161 actcgttttg tatgaattat gatagcattc acatcgctgg taataaaatg ttcattttcg
     2221 ccaatcccaa taatgagcgg actaccttta cgcactgcaa ttaatttatc cggttctttt
     2281 atagatatga caaccaatcc ataagtgcct tctactcttt tcattgcctc acaaacagca
     2341 tcttcaagag ttggttcgct aactaaatac tgttctatta gatgaacaat aacttctgta
     2401 tctgtttcgc ttataaaagt atgccccagt tccaataatc tatcttttaa tattttatag
     2461 ttctctatta tgccattatg gacaatggca atttcccctt tacaatccaa atgaggatga
     2521 gcattaactt catttggttc tccatgggtt gcccaacgag tatgtgctat agcaatattt
     2581 ccactacatt tatttggttc tggcaaacag cgttccaatt cgataatttt cccttgcctt
     2641 ttatatactt gcaattcatc tttatgaata agagcacaac cagaactatc ataacctctg
     2701 tattctaacc gcttaattgc ttctacaaca ataggtaaag cattacgata gccaatataa
     2761 ccaactattc cacacatatt tcttctcctt gggcagtaac tttatgaaaa gcataaagag
     2821 agataacaaa aacaactaaa gccggtataa ataaccaata gtgtatatta tccccctccg
     2881 atactaagaa tttaacccaa ctagaattgc tatatgtaac cagtataaaa gctaatccat
     2941 tatttattat atgcgataac atactggtat aaactgaacc cgaacgcaaa gccaaatatc
     3001 ctaataaaaa acccaaaagt aaaaccggaa caaaacgaaa aggatcaaga tggaaagcag
     3061 caaatagaat tgcactgata attactgccc attgtaaacc gtatttttca aaaaaacgaa
     3121 ttaaaaatcc tcggaataaa agttcttcac aaattccagg caacaaagct attgccaaaa
     3181 agactttcca gggagcttca ttcatattaa aaagctgagt aagtgcctct gtgtattttt
     3241 ccggataagg aaaaatcgta tttattagct gtgacatgat agatacaatt atcgaagcag
     3301 atatagctat aaaaggaaca aggataagac cagctggttt gggagtttta aatcttaaga
     3361 tttctttcgg tttcagcttt aatagcttca aaactataaa aacgggaaga gcaataataa
     3421 tgagctcagt ttggattaat ccattataca ggtctttagt ttgccaatat ccacctaaat
     3481 agtataataa aattaaagct attgtaaaat aaactatacc gttgtatgga gttaaggcat
     3541 atttgggttt ctttttcaga acttttatac ctccactttc ttcttcagta cggaaaagaa
     3601 ctgattctgt tttaaacagc tttacagtga tcagaatggc aattagatct aaaactaaag
     3661 tgcttattat ggtaattaag atatgcgaca gagtatattc attaatcaaa accgctttga
     3721 atagtaaagc tatattaaca acaggaacta tagccagcag attggaaatt tctatagtag
     3781 gaataaaact gaccatcccc atcagcattg ctatcattaa taatggttgt tcgtaacttc
     3841 ttgcttcttt gatattacgg ctgaaagtag aaatggaaac aagcaaagca gcaaatagag
     3901 ttgctaaagg aatcattgca ataagtaaaa tcaacattgc attaatgggc aaagttacac
     3961 ctgacatttc tttactctga tttgccagca taaactgtaa ggaaaaactg atgctgaaaa
     4021 gatttacaat catattaagc atagccaaag ttatgatagt gagatattta cccataacaa
     4081 tctctgtcct tccaacagaa gaaacaagta aagtctccaa ggttttcctt tctttttcg
//
LOCUS       DAORHB010000127         4132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_23342_length_4132_cov_3.950454, whole genome shotgun sequence.
ACCESSION   DAORHB010000127 DAORHB010000000
VERSION     DAORHB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23342_length_4132_cov_3.950454"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /gene="rplS"
                     /locus_tag="PKM80_04495"
     CDS             complement(<1..235)
                     /gene="rplS"
                     /locus_tag="PKM80_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNV93202.1"
                     /translation="MDILQQVGRDQIRTDLPDYRVGDTVKVHYKIKEGNKERIQVFQG
                     IVIQKKGAGVSKTFTVRKVSSGVGVERIFPQNSP"
     gene            complement(456..1127)
                     /gene="trmD"
                     /locus_tag="PKM80_04500"
     CDS             complement(456..1127)
                     /gene="trmD"
                     /locus_tag="PKM80_04500"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNV93203.1"
                     /translation="MIFEVLSLFPDFFSGFLTSSIIFRAIENELLQVKLTDYRTYSKN
                     RHHQVDDYPYGGFPGMVIQAQPIYDALQELLLEGNAPVIYFTPQGRSLNQQILQNYTS
                     YERIILLCGHYKEIDQRVRDLCISDEISIGDYVLSGGEIPALVFMDGISRLLPGVLSD
                     IESANSDSFSSGGLGFPCYTRPDIFMGLKVPDILLSGNHKLIKQWASEQGILLTRTRR
                     PDLIK"
     gene            complement(1124..1654)
                     /locus_tag="PKM80_04505"
     CDS             complement(1124..1654)
                     /locus_tag="PKM80_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93204.1"
                     /translation="MNHPFLTGIGKLGRQDADGFHPVMIKPEFIAVFSELEELYLIFN
                     SDRVFYVTISERKIKDKKTWIKLKEDGIAEEQYLHKDVVIAIDAQDTESKGTSLDYLL
                     GYQAIFADEEFGIIEDFFFNGAQQVLQIVDKNKIEYLVPFVDYYIDTILNNPGCVILK
                     NALELIAFYRAEAKSK"
     gene            complement(1655..1885)
                     /locus_tag="PKM80_04510"
     CDS             complement(1655..1885)
                     /locus_tag="PKM80_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNV93205.1"
                     /translation="MKELIEFIVKALVDDPSEVNITEITGDKITLYELRVSKADIGKV
                     IGKRGRTASAIRTIINAVSTKQGKRAELEIIE"
     gene            complement(1907..2200)
                     /gene="rpsP"
                     /locus_tag="PKM80_04515"
     CDS             complement(1907..2200)
                     /gene="rpsP"
                     /locus_tag="PKM80_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNV93206.1"
                     /translation="MVKLRLRRMGANDQPFYRIVAVDSREKRDGKYIECVGWYDPKPD
                     PSKINIDTERAIYWLSVGAQPSDTVSSLLRKAGILQIWHERKLANQKTADAEK"
     gene            complement(2302..3624)
                     /locus_tag="PKM80_04520"
     CDS             complement(2302..3624)
                     /locus_tag="PKM80_04520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNV93207.1"
                     /translation="MKIAVIGSGYVGLTTGACFAEMGNTVICVDKDEAKIKLLNSGKV
                     PIFEPGLQEMISRNMAAKRIIFTLDLKYAVQESHIIFIAVGTPPGEDGSADLQYVICA
                     AEEISGCMNEPKIIVNKSTVPVGTADIVRAKIQEVLDSRGLELPFDVVSNPEFLKEGA
                     AIDDFMKPDRVVIGTDNPEAGEIMRTLYEPFCRTNDRVLIMNIRSAEMAKYAANAFLA
                     TKISFINEISRLCDAYEADISEVRNGMCSDSRIGYKFIFPGVGYGGSCFPKDIKALIY
                     MSQKVGYNPLILTAVEEVNNYQKRVLVEKVVRHFGSNLSGKTFAVWGLAFKPQTDDMR
                     EAPSIVIINELLAMGAKIKAYDPVAMDEAKKIFGNNNVVTLCADEYETLKDTDAMLLI
                     TEWHQFRYPDFERMSKIMRQKVIFDGRNQYNPKAIKEMGFSYYGIGRR"
     gene            complement(3621..>4132)
                     /locus_tag="PKM80_04525"
     CDS             complement(3621..>4132)
                     /locus_tag="PKM80_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV93208.1"
                     /translation="AINKSVGEDYLYFYRYQYGLEYTILRYANVYGPRQISSGEAGVV
                     SLFTEKLLKGDIPTVYRYDGEPDGMIRDYVYVGDVVKANLLALEIGEGDAFNIGTCLE
                     TKTRDLYWEIANQLGVNEEPHYGVARKGDLHRSLLSYEKAKRVLGWEPKIGLSEGISQ
                     VIKYIKELK"
BASE COUNT         1255 a          890 c          629 g         1358 t
ORIGIN      
        1 tcggtgaatt ttggggaaat atcctctcca caccaacacc actggaaact ttacgtacag
       61 tgaaagtttt tgaaacgcca gcgccttttt tttgaataac gataccctga aagacctgaa
      121 tgcgttcctt atttccttct ttaattttat aatggacctt tacggtatcg ccaacgcgat
      181 aatccggtaa gtctgttctg atttggtctc tgccaacttg ttgcagaata tccatttgtt
      241 gggttcctcc catatatata ctcgggagaa aaaaacctat tgtgatcaag ggagaacgga
      301 ctaaaaataa attctctata aaccgccctg ttatttctat cctcattaga tgcattgcta
      361 caataagata aggcccacag agatataatt tcccttcagg catattcctt gcaatcacgc
      421 ataagttcag gtttctttct tcctgcttgc ttgtcttact ttatcaaatc tggtctgcga
      481 gttcttgtaa gtaatatacc ctgttcactt gcccattgtt tgatcaattt atggttgcca
      541 ctaagtaaaa tatccggaac ttttaaaccc ataaatatgt caggtcgtgt ataacaggga
      601 aaacccaatc ccccactgct gaaagaatca ctatttgcac tttctatatc acttagtact
      661 cctggtagca atctgctaat tccatccata aaaacaagtg ctggaatttc tcctccgctt
      721 aaaacataat ctcctataga aatttcatcc gaaatacaaa ggtctcgaac tctttgatca
      781 atttctttgt agtgaccaca gaggaggatt atccgttcat aggatgtata gttttgcagt
      841 atctgctgat ttaaacttct accttgagga gtaaaatata taactggagc atttccttcc
      901 agcaaaagtt cttgcaaagc atcataaatt ggctgtgctt gaataaccat accgggaaag
      961 ccaccatagg gataatcatc tacttgatga tgtctatttt tagaataagt gcgataatcc
     1021 gtcagtttca cctgtagcag ctcattttct atcgctctaa atataatgct gcttgttaaa
     1081 aagccggaaa agaagtccgg aaaaagagaa agtacttcaa agatcatttc gattttgctt
     1141 ctgcccgata aaaggcaatg agctctaatg catttttcaa aattacacag ccaggattgt
     1201 taaggatcgt atctatataa taatcaacaa aaggaactag atattcaatt ttattcttat
     1261 ccacaatctg taaaacctgc tgagcgccat tgaaaaagaa gtcctctatt attccaaatt
     1321 cttcatctgc aaaaatagct tgataaccta aaagataatc caacgatgta ccttttgatt
     1381 cagtatcttg tgcatcaata gcaattacta catctttatg tagatattgc tcttcagcga
     1441 ttccatcttc tttcagttta atccaggttt ttttatcctt tatttttctc tcactgatag
     1501 ttacataaaa cactctatcg ctgttaaaga tcaaatacaa ttcttcaagc tcagaaaaca
     1561 cagctatgaa ttctggcttg atcatcacag gatgaaagcc atctgcatcc tgcctaccca
     1621 gtttgcctat tccagttaaa aaaggatgat tcatttactc gataatttcg agctcggcac
     1681 gcttcccctg ttttgtacta acggcattaa taattgttct gatggcactt gcggttctac
     1741 cccgtttgcc aataaccttt ccaatgtcag ctttcgaaac cctaagctca taaagggtaa
     1801 ttttgtcgcc agtgatctcg gtaatattca cttccgaagg atcatcaacc agagctttaa
     1861 caatgaattc aatcagttct ttcatttttc acctcagcta atctatttat ttttctgcat
     1921 ccgcagtttt ctggtttgca agtttccgtt cgtgccaaat ttgaagtata cctgcttttc
     1981 ttaacaatga agaaacagta tcagatggtt gtgcaccaac acttaaccag taaatggcac
     2041 gctctgtatc tatgtttatt ttggaagggt cgggttttgg atcataccaa ccaacgcatt
     2101 caatatattt tccatctcgt ttttcgcgag aatcaactgc cactatacga tagaagggtt
     2161 gatcgtttgc tcccatcctt cttaatctca gttttaccat tagttctcct tctaattata
     2221 acaatatttt accaagtttt gatggaagga ctttccgtca agacaatttt tttatccgct
     2281 gctaataaat atttattatt actatcttct accaatccca taatagctaa aacccatctc
     2341 tttaatagct ttgggattat attgattacg cccgtcaaag ataacttttt gacgcataat
     2401 tttactcatc ctttcgaaat ctggataacg gaactgatgc cattctgtaa ttagtaacat
     2461 tgcatctgta tctttaaggg tttcatattc atcagcacaa agagttacaa cattattgtt
     2521 cccgaatatc tttttggctt cgtccatagc tacaggatca taggccttta tttttgctcc
     2581 catagcaaga agttcattga tgattactat ggaaggtgct tctcgcatat catctgtttg
     2641 tggtttaaaa gctaaacccc aaactgcaaa ggtttttcca gataaattac taccaaaatg
     2701 acgaactact ttttccacta aaaccctctt ctgatagttg ttaacttctt ccaccgctgt
     2761 taagataaga ggattgtaac ccactttttg cgacatataa ataagggctt ttatgtcttt
     2821 ggggaaacaa ctgcctccat aaccaactcc aggaaaaatg aatttgtaac cgattcgaga
     2881 atcactacac attccatttc gaacttctga tatgtctgct tcataagcat cgcaaagacg
     2941 agatatttcg ttaataaatg atatctttgt agccaaaaaa gcattagctg catattttgc
     3001 catttcagct gaacgaatat tcataatcaa aaccctatca ttagttctgc aaaacggttc
     3061 gtataaggtt cgcataattt ctccagcttc aggattatct gtaccgataa caactctatc
     3121 aggtttcata aaatcatcta ttgcagcgcc ttctttcaaa aattcaggat tggatacaac
     3181 atcaaaaggt agttccaaac cacgagaatc gagaacttct tgaattttgg cacgcactat
     3241 atctgcagtc ccaactggaa cagtggattt attaactatg attttgggct cattcataca
     3301 accagaaatt tcttcagcag cacaaattac atattgtaaa tcagcagaac catcttctcc
     3361 aggaggagtt ccaactgcaa taaagataat atgtgattct tgaacagcat atttgaggtc
     3421 taaagtaaaa ataatacgtt ttgcagccat attgcgactg atcatttctt gcaaacctgg
     3481 ctcaaatatt ggaactttac ccgaattgag taactttatt ttagcttcat ccttatctac
     3541 acagataacc gtgttcccca tttctgcaaa acaggcacct gtagttaaac ctacatagcc
     3601 actaccaata actgcaattt tcatttgagc tccttaatgt acttaataac ttgtgaaata
     3661 ccttcactaa ggcctatttt gggttcccaa cccaatactc tttttgcttt ttcatatgaa
     3721 agtaaggaac ggtgtaaatc cccttttcga gcaacgccgt aatgtggttc ttcgtttaca
     3781 cctaactggt tagcaatttc ccaatataaa tctctggttt tggtttcaag gcaagttcca
     3841 atattaaatg catctccctc acctatttcc aaagcaagta aattagcttt aactacatct
     3901 cctacatata cataatccct aatcattcca tctggttcac catcataacg ataaactgta
     3961 ggtatatcac ctttcaataa tttttcggta aaaagagata caacaccagc ttctccactg
     4021 gaaatttgac ggggaccata aacattggca taacgtaaaa tagtatattc aagcccatat
     4081 tgataacgat agaaatataa ataatcctcc cctacacttt tattaatagc at
//
LOCUS       DAORHB010000128         4027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_24234_length_4027_cov_5.057905, whole genome shotgun sequence.
ACCESSION   DAORHB010000128 DAORHB010000000
VERSION     DAORHB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24234_length_4027_cov_5.057905"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PKM80_04530"
     CDS             complement(<1..969)
                     /locus_tag="PKM80_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNV93209.1"
                     /translation="MSKIIDLKNTATPDEWNDWHWQIKNRITSYEQLAKYIELQPEEE
                     AVFKDKAFSFRMAITPHYLSLIDQSNPYDPIRLQAIPRIAESHISSSDMADPLSEDAD
                     APVPGMTHRYPDRVLLLLTDQCAMYCRHCTRRRKAGEYDAPMPKENVDKALDYIKEHK
                     EVRDVILSGGDPLTLSDERLDDILNKLSKIEHIEIVRLGTRTPVVLPQRFTDSLLTIL
                     KKYKFVWLNTHFNHPNELSEDSCKALAKIAETGIPMGNQSVLLKGINDNVDVMKALVH
                     KLVKNRVRPYYIYQCDLSEGISHFRTPISKGIEIIESLRGHTSGLCI"
     gene            complement(984..2042)
                     /locus_tag="PKM80_04535"
     CDS             complement(984..2042)
                     /locus_tag="PKM80_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNV93210.1"
                     /translation="MKSNGCRYGTHRVIEPKGVLPQPAKVLNNDMSEIWDNEMLIDVI
                     RLNIDSASFHQIKNKLIAKGFTDLEKAFAEHAIDLLNRTGKHKNEDTGSGGMLIGRVA
                     AIGDKFEMKEEVKVGDKIASLVSLSLTPLKINKVKKVLLDKDQMEIEGQAILFSSGIY
                     AKLPDDLDENLALSVLDVAGAPAQVERLVKPGDTVVIIGANGKSGILCNAVAKERAGI
                     CGKVIGVVRKESYIPTCKTTGCDEVILASATDAITIQKEVSRLTNGKMADVVINVVNT
                     EDTELPSIMAARERGLVYFFSMATSFTKAALGAEGIGADVDMMIGNGYAKHHSEIALD
                     LLRRNPVLMKIFKERYAE"
     gene            2504..2818
                     /locus_tag="PKM80_04540"
     CDS             2504..2818
                     /locus_tag="PKM80_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93211.1"
                     /translation="MIITFSVQNFLSLRNKVSLDFRTTSDKTLEDYYVVSTEKPKLRI
                     LRMAMIYCADASGKTNLLMILDFIKRFTSNTSINKELPILITPFALDKDKQLNFSSGE
                     EV"
     gene            2981..3712
                     /locus_tag="PKM80_04545"
     CDS             2981..3712
                     /locus_tag="PKM80_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93212.1"
                     /translation="MKTFKFVLVSLCIMSMLTSWVSIEAKKKEEDKGKEVESKLFLDD
                     SKLDNQDYYNQFPSSAIQSFENALLTPKVSDIKIITINSDQTKTDAKIAEEMIAVALG
                     MRQEYVNLEAEGKSLLSGNLEGSELEISEMVKAKLEDLKNAKAQLSESQKVYFLSGFT
                     YLASSIVREKVILESAKMYVDEASKATGMAKMKYAKDLPYATAVVTDIPILLESQIKT
                     INEFATIAKTQSIKIPENVTKSLFE"
BASE COUNT         1304 a          786 c          634 g         1303 t
ORIGIN      
        1 aatgcataat ccggaagtat ggccacgcaa actttctatt atttcaatac ctttggatat
       61 gggagttcga aaatgactga tcccttcaga aagatcacat tgataaatat aatagggacg
      121 aactctgttt ttaaccagtt tatgcaccaa tgctttcatt acatccacat tatcatttat
      181 cccttttagt aaaacggatt gattacccat cggaatccct gtttcagcaa ttttggcaag
      241 tgctttacag gagtcctcac taagctcatt aggatgatta aaatgcgtat tcagccacac
      301 aaatttatac tttttaagga tcgttaaaag tgaatcagta aagcgttgcg gtaaaaccac
      361 cggagttctt gttcccaaac gcacaatttc aatatgctca attttactca gtttatttaa
      421 tatatcatct aaacgctcat cacttaaagt gagaggatcg cctccactta aaataacatc
      481 gcgcacttcc ttatgctctt ttatatagtc caatgcctta tccacatttt ctttgggcat
      541 tggggcatca tattcacctg cttttctccg gcgggtacaa tgtctacaat acatagcaca
      601 ttgatctgta agcaaaagca aaacccgatc tggatagcga tgagtcattc caggaaccgg
      661 agcatcagca tcttcactta aaggatctgc catatcagaa gatgaaatat ggctttccgc
      721 aatccgcgga attgcctgta aacgaattgg atcataagga ttgctctggt caatcaaaga
      781 aagataatga ggagtgatag ccatccgaaa agaaaatgct ttatctttga aaacggcttc
      841 ttcctcgggt tgaagttcaa tatatttagc taattgttca taagatgtta tccgattttt
      901 tatctgccaa tgccagtcat tccattcatc tggagtggca gtgtttttaa gatcaattat
      961 cttgctcatt ttgtcctctt ttcttattcc gcatagcgtt ctttaaaaat cttcattaaa
     1021 acaggattac gccttagtaa atccaatgca atctcagaat gatgtttagc ataaccattg
     1081 ccaatcatca tatctacatc agcaccaata ccttccgcac ctaaagctgc tttggtaaaa
     1141 gaagtagcca tagagaaaaa atagaccaaa ccacgttctc tggctgccat aatacttggc
     1201 aattcagtat cttccgtatt cactacattg ataactacat ctgccatttt accattagtt
     1261 aaacgcgaga cctctttttg aatagtgatg gcatccgtag cgctggctag aataacttcg
     1321 tcacaaccag ttgtcttaca agtaggtatg tagctttctt tacgcacaac cccaataact
     1381 tttccgcaaa ttcctgcccg ctctttagcc acggcattgc ataaaattcc gctcttccca
     1441 ttagcaccaa taattacaac tgtatctccc ggctttacta aacgctcaac ttgagctgga
     1501 gcgcctgcaa catccaaaac agaaagtgcc aaattttcat ccaaatcatc tggtagcttg
     1561 gcataaatac cactgctgaa aagaattgcc tgaccttcaa tttccatttg atccttatcc
     1621 agcagaactt ttttcacttt gtttattttg agaggagtta gcgaaagcga aaccagagag
     1681 gcaattttat ctccaacttt cacttcttct ttcatttcaa acttatcccc aatggcagca
     1741 actctgccaa ttagcattcc gccagaaccg gtatcttcgt ttttgtgttt accagttcta
     1801 ttaagaaggt caatagcatg ttccgcaaat gccttttcca aatctgtaaa acccttagca
     1861 atgagcttat ttttaatctg atgaaaagaa gcagaatcaa tatttaaacg aatcacatca
     1921 atcagcattt cgttgtccca aatttcgctc atatcgttat ttaaaacctt tgcgggttgc
     1981 ggtaaaacac ctttgggttc aattacccta tgcgttccat atctacatcc attacttttc
     2041 attttcttct cctctttgaa tgtctatctt attatttata tttatgttaa ctatgaattt
     2101 ccactttttt gtatttgccc ttgctgtcaa tccttttgta tagatcaact tctggcaagt
     2161 cccataatct cttgcaacta tcaaagaaaa atttatatcc aaattgaatt cagataagta
     2221 aagataaatt cccaaaggaa tagtcaatta cgatttgctg tagaaactaa aacacttcaa
     2281 aaagcacatt cttcaaatca gctgccaaat ctaaatcaat aactttatag cataaccttc
     2341 tcttttcatt aatcctttta gcagagagta agtttagaaa tacaattcaa gccgataaaa
     2401 catcatattt gcagaataac tgtaaatttc tacttgacat tctcccatgt ctaattaaat
     2461 tgtctatgaa atgcagaaaa agtgcataac aaggagtcaa aaaatgatta tcacctttag
     2521 cgttcaaaac ttcctttccc tccgaaataa agtaagcttg gattttcgta ctaccagtga
     2581 caaaaccctg gaggattatt atgtagtttc cactgaaaaa ccaaagctaa gaattcttag
     2641 aatggctatg atctattgtg ctgatgcctc tggaaaaact aatctactta tgattttaga
     2701 ttttataaaa cgatttacta gtaatacttc tataaacaaa gaacttccta ttctaataac
     2761 cccttttgct ttggataaag ataaacagtt aaattttagc agtggagaag aagtttaaat
     2821 gaatatctgt ggttaagtac cttatcgatt taggggtttg gaatctaatc cgacagttgc
     2881 taaaaccaat actttatttg aaacagtaaa aaatatatct caatatttag ttgaccagcc
     2941 ttgagtaaaa ttgagttata ataaaaaaaa ggagcgaaaa atgaaaacat ttaagtttgt
     3001 acttgtatct ctttgcataa tgtcaatgct tacatcttgg gtaagcattg aagcgaagaa
     3061 aaaagaagaa gacaaaggga aagaagtaga atcaaaactt tttctcgatg actcaaaact
     3121 ggataatcag gactactata atcaatttcc atccagtgca attcaatctt ttgaaaatgc
     3181 tctattaact cctaaggtct cagatattaa aataataact ataaattctg accaaacaaa
     3241 aaccgatgct aaaattgctg aagaaatgat tgctgtcgca ttgggaatgc gtcaggaata
     3301 tgtgaattta gaagcagaag gcaaatctct actttctgga aatctggaag gaagtgaatt
     3361 agaaatttcg gaaatggtga aagccaaact ggaagaccta aaaaatgcta aagcccaatt
     3421 gagtgaatcg caaaaggtct attttttaag tggctttact tatcttgcta gctcaattgt
     3481 aagggagaaa gttatcttag agagtgctaa aatgtatgtg gatgaagcta gtaaagccac
     3541 aggtatggct aaaatgaaat atgctaaaga tttgccctat gccactgctg tagtaactga
     3601 catacctatt ctgcttgaat ctcaaataaa aactattaat gagttcgcta ctattgctaa
     3661 aactcagagc atcaaaatac ctgaaaatgt cacaaaaagt ttgtttgaat aataatttgg
     3721 atatttaatc gaaaaaaacc aatgctaacg cagtaaagga atgctggtcg cctttactgc
     3781 tttaagcttt aaggagcgga aactctattc tgctaacatt caccactttt ctggtataac
     3841 tctttgattt ttatgtattg tcattcattt agatactttt gcttgtccga aaaagttccg
     3901 taggaacgac agaattatta ttttctctga taaaattttc attttttctg actaataact
     3961 ttctccgtta tatgtcttat ctattttatt gtatttctgt ctttttggtc ttttggtttt
     4021 taacttt
//
LOCUS       DAORHB010000129         4019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_24324_length_4019_cov_3.895308, whole genome shotgun sequence.
ACCESSION   DAORHB010000129 DAORHB010000000
VERSION     DAORHB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24324_length_4019_cov_3.895308"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PKM80_04550"
     CDS             complement(<1..904)
                     /locus_tag="PKM80_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNV93213.1"
                     /translation="MLGRTKKIHFVGIGGIGMSGIAEFLHNQGLVITGSDLKKSEITA
                     HLEALGIKVEEGHRPENLGDADVVVKSSAVTDDNPEILAAKEKKIPVIRRAEMLAEIT
                     RMSFSIGISGTHGKTTSTSMAGSVLESAGLEPTIIVGGKVKNFGSSNVMGSGKYIVVE
                     ADEYDHSFLALTPCIAGITNVDVDHLDCYQDLDHIKSSFIEYANKAPFFGSVIACLDD
                     PGVQSILPFINKRIVTYGFSRQADVQARDIEMQGFKSSYDLSYKEYKLGRINLNVTGK
                     YNIQNSLLAASIGLELDIPFTAIQL"
     gene            complement(904..1986)
                     /gene="murG"
                     /locus_tag="PKM80_04555"
     CDS             complement(904..1986)
                     /gene="murG"
                     /locus_tag="PKM80_04555"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNV93214.1"
                     /translation="MKFIFGAGGTGGHITPALALADELQKYNHDILFIGNRNSIEESL
                     CKSAGYPIRYIKVQKLYRSFKLENLVFPFFLASSIIAAISILKKEKPQAVICTGGFVA
                     GPTAIAASLLKVPLFFHESNSYPGLVTRKMAKKINTIFISFENSKKYLVKAKTVNYGI
                     PLRQSKISSTQKTFELSSLGLDNNKPTIIVSGGSQGSIAINTVVSSAIPVILSMGYQI
                     IWQTGKTSYERFATKHKNTEGVYMFAFSPELPKMLSKCKLAITRAGAMTIAELEENCI
                     PAILIPLPTAAENHQYYNALAQQEKGVAMLLEQKDLSLLTLVESLKKMSENLAYYQDN
                     LFSLPKNQAAEKIVHYIINYLQNKGE"
     gene            complement(1983..3122)
                     /locus_tag="PKM80_04560"
     CDS             complement(1983..3122)
                     /locus_tag="PKM80_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNV93215.1"
                     /translation="MKRSKIQSYIVSFDKVILITYCLLCVIGLVVMLDISSVQSSMRY
                     FYRQLFFMIISAIFAIVILYTLNLEKLRVLSPYIVYLTILLLIIVLVKGNTVKGATRQ
                     INLGFINFQPSVLARLALIFYFAHILDKKNEELVSSTTGIFLGNFFALIVVTGVTFLL
                     IIMERHLSTLIIGGLTLYGMLIYAGTKKRVLITIAIIGIIAGCLVLANGADYRKGRLT
                     TYKKFCLFLKPAGEIKIEDNDYQVKESLTALSSGAIIGTGMARGRAKHYYLPEARTDY
                     VYTVIGEEWGFVGALIVFGLHCFLFFRCFWMAHSQENRFLEFLGVGLAMNIFCNVLVN
                     TGVAMSLLPPTGNTLPFISYGGSALLIDSIALGMLLNISAQRRYV"
     gene            complement(3109..>3780)
                     /locus_tag="PKM80_04565"
     CDS             complement(3109..>3780)
                     /locus_tag="PKM80_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNV93216.1"
                     /translation="TKNIKASMLYFSSHQIETAYAYPVDNYIQLGPTTKVPLSNLEIR
                     GPHNYANAMAAMLAVYALYPEPAKIAEAITGFKPLPHRLEFVGSVNGISFYNDSKATN
                     TDSVRSALHSFDKPIRIIMGGSDKGESYSTLTSDMQKIVKKAYLTGDTSEKMYQTWNG
                     IIPLECISDFEDCVTKAYNDSLPGDIIVLSPACASFDKFKNYEERGEFFKLVVNRIIK
                     ENEKK"
     assembly_gap    3781..3790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3791..>4019)
                     /locus_tag="PKM80_04570"
     CDS             complement(<3791..>4019)
                     /locus_tag="PKM80_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNV93217.1"
                     /translation="KPGLDYIVLEISSFQLDLIDTFKPNVAVLLNITPDHINRYNTFE
                     DYALSKVNIFKNQTATDTAVLFMDSPEIMLYT"
BASE COUNT         1261 a          776 c          700 g         1272 t
ORIGIN      
        1 ctaactgaat agcagtaaaa gggatatcca attccaagcc aatagaagct gccagtagag
       61 aattttggat gttatacttg ccggtaacat tcaaatttat gcgccccagt ttatactcct
      121 tataggacag atcataggaa gatttgaagc cttgcatttc gatatctctg gcttgtacat
      181 ctgcttgtct tgaaaacccg taagtaacta tccttttatt aataaagggc agaattgatt
      241 gtactcctgg atcatccaaa caggcaataa cgcttccgaa gaagggtgct ttattagcat
      301 attcaatgaa agaactttta atgtgatcca aatcttgata acaatcaaga tgatctacat
      361 ctacattggt tatacccgca atacaaggag ttaaagctag gaatgagtga tcatactcgt
      421 ctgcttctac aactatatac tttccgcttc ccatcacatt ggagctacca aagtttttta
      481 ctttccctcc cacaatgata gttggttcta aaccagcgct ttctaaaact gaaccagcca
      541 tagaagtaga agttgttttt ccatgcgttc cagaaatacc gatggaaaag ctcattctgg
      601 tgatttcagc taacatttca gcgcggcgga taacaggaat tttcttttct tttgctgcca
      661 atatttcagg attatcatct gttacagcac tggattttac tacaacatct gcatctccaa
      721 ggttttctgg tctatgtcct tcttcaactt tgattcctaa cgcttccaag tgtgctgtaa
      781 tttctgattt ttttaggtct gaaccagtaa ttaccaaacc ctgattatgt aaaaattctg
      841 caattccgct cattccgatt ccgccgattc cgacgaaatg tatttttttt gttcttccga
      901 gcattattcc cccttgtttt gcagatagtt aattatataa tgaacgatct tttcagctgc
      961 ctgatttttg ggaagcgaga acagattatc ttgataatat gccaggtttt cgctcatctt
     1021 tttaagactc tcaaccagag ttaagagtga taggtctttc tgttctaaaa gcatagcaac
     1081 tcccttttct tgctgagcca gagcattata gtattgatga ttttctgccg cggttggaag
     1141 tggaataaga atagcaggaa tacagttttc ttccagttcg gcgatagtca tcgcgcctgc
     1201 acgcgttatt gctaatttgc atttagaaag catcttggga agttcgggcg aaaaggcaaa
     1261 catataaaca ccttcggtat ttttatgttt ggttgcgaaa cgctcgtaac tagttttacc
     1321 cgtttgccag attatttgat atcccataga taatataacc ggtatggcag aactgacaac
     1381 agtattaatt gcaattgaac cttggctgcc tccactaact ataatagtag gtttattgtt
     1441 atccaggccc agagagctta gttcaaaagt tttctgggta ctgctgattt tgctttgtct
     1501 aagaggaatt ccatagttta cggttttagc tttaactaag tatttcttgc tattttcaaa
     1561 cgagatgaag atagtattta tcttttttgc catcttccga gttaccaaac cagggtaact
     1621 attgctttca tggaagaaca aagggacctt taataaagaa gcagctatag ctgtaggtcc
     1681 ggcaacgaaa ccacctgtgc aaattactgc ttgaggtttt tcttttttta agatgctgat
     1741 tgcagcaatg atactgctgg ctaaaaagaa agggaacacc aaattttcca atttaaagga
     1801 gcgatataat ttttgtactt ttatatagcg aatgggataa ccggctgatt tacaaagaga
     1861 ttcctcaata ctattgcgat tgccaataaa tagaatatcg tggttatatt tctgtagttc
     1921 atctgccaaa gctaaagctg gagtgatgtg tccacccgtt ccacctgcac caaaaataaa
     1981 cttcatacat atctccgttg ggcactaata tttaacaaca ttcccaaggc gatggaatca
     2041 atcaacagag ctgaaccacc ataactgata aatggtaagg tgtttcctgt gggaggtaaa
     2101 agagacatag ctacacctgt attaactaaa acattacaga atatattcat tgctaaaccc
     2161 actcccagaa attctaaaaa tcgattttct tgagaatgtg ccatccaaaa acagcggaaa
     2221 aagagaaaac aatgtaagcc aaaaacaata agagcaccaa caaatcccca ttcttcacca
     2281 attaccgtat atacataatc tgttctggct tcaggaagat aataatgttt tgctcttcct
     2341 ctggccatac ctgttcctat aattgctcca ctggaaagag ctgttaaact ttccttcacc
     2401 tgataatcat tatcttcaat tttgatttca cctgcgggtt ttaagaacag acagaacttt
     2461 ttgtatgttg ttaagcgtcc ttttctataa tctgctccat tagctaaaac gaggcatcct
     2521 gcaattatgc caattatcgc tattgtgata agaactcttt ttttagttcc agcatagata
     2581 agcataccat agagtgttaa tccacctata attaaagtac ttaaatgccg ttccataata
     2641 attaacagga aagtaacccc tgtaaccact attaaggcaa agaaattacc taaaaaaata
     2701 ccagttgtag agcttaccag ttcttcattc tttttatcta agatatgagc aaaatagaaa
     2761 attagagcca gacgcgccaa aacgcttggt tggaaattta taaaacctaa gtttatttga
     2821 cgagtagctc ctttaacagt atttcctttc accaaaacta taatcagtag aagaatggtt
     2881 aagtacacaa tatagggaga aagcactcta agtttttcca aatttagagt atataaaata
     2941 acaatagcga aaatggcaga gattatcata aagaaaagct gtctatagaa atagcgcata
     3001 gaactctgaa cagacgaaat atccaacata accacaagcc cgataacgca caataagcaa
     3061 taagtaatta aaattacttt atcgaagcta acgatatagc tttggatttt acttcttttc
     3121 attttccttg ataatcctat ttacaacaag cttgaagaat tctcctcgct cttcataatt
     3181 tttaaattta tcaaaactgg cacaggcagg cgaaagaacg attatatcac ctggaaggga
     3241 atcattatat gctttagtta cacaatcctc aaaatcagat atgcattcaa gagggattat
     3301 tccgttccag gtttgataca ttttctctga tgtatcacct gtcagatatg ccttttttac
     3361 tattttttgc atatcggaag ttaaagtact atagctttca cctttgtcgg agccccccat
     3421 aataattctg atgggtttat caaaactatg caaggcactg cgaaccgaat cggtattagt
     3481 agctttggaa tcattataga aggaaatgcc atttacgcta cccacaaatt ccaaacgatg
     3541 aggcagaggc ttaaaacctg taattgcttc agcaattttt gctggttctg gatataatgc
     3601 ataaactgca agcatagcag ccattgcatt agcataatta tgaggtcctc taatttccag
     3661 attggaaagt ggaacttttg tagtaggccc caattgtata tagttatcta ctggataagc
     3721 ataagctgtt tctatctgat gtgaggaaaa atataacatg ctagctttta tattcttagt
     3781 nnnnnnnnnn tagtatatag cataatttca ggagaatcca taaagagcac agctgtatct
     3841 gttgctgttt gattcttgaa gatgttcacc ttactaagag cataatcctc gaaggtattg
     3901 tagcgattga tatggtcggg agtaatattt aacagtacag ctacattcgg cttgaaagta
     3961 tctatcagat ctaattgaaa actgctgatt tccaaaacga tataatctaa ccctggttt
//
LOCUS       DAORHB010000130         3959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_24835_length_3959_cov_5.531506, whole genome shotgun sequence.
ACCESSION   DAORHB010000130 DAORHB010000000
VERSION     DAORHB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24835_length_3959_cov_5.531506"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="PKM80_04575"
     CDS             complement(<1..1788)
                     /locus_tag="PKM80_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV93218.1"
                     /translation="MSKYLIVGGVAGGATTAARLRRLDEKAEIIMFERYSYISYANCG
                     LPYYIGGVIKERDRLFVQTPESFKARLNVDVRINQEVIAINRKEKTVTVKRADGSQYN
                     ENYDKLVLSPGAEPLIPPLPGISDSAIFTLRNVSDTDKIKEYIESHKVQHCVIIGAGF
                     IGLEMAENLHNLGMKVTLVEMAEQVMTPLDYEMAAIVHQHLKTKEVEFYLQDGVSSFS
                     RNDGSLQVHLQSGRTLNADMVLLSIGVRPDSKLAEAAGLELGINKGIRVNSYLQTSDP
                     DIYALGDAIAYPNPITKMESLTYLAGPANKQARIVADNIVKGNIRKYKGSIGTAIAKV
                     FDITVGSTGVSEKVLKDAGIDYISSIIHTSSHAGYYPDALPITIKIVFNPKDGKLLGA
                     QIVGFDGVDKRTDMLAHILKHNETIYEIQEIEHSYAPPFSSAKDAVNIAGMVADNIIN
                     GLVKIIHWDDLSSLDKNDTIIIDVRTPDETALGTIAGSINIPLDDLRSRLKEIPENKQ
                     VVVFCGTGLRSYFAARILMQNGFKNVYNLSGGYLTYEYATQKQSNEDIFADDYIGKDD
                     DIYKGKEEKMDRDIKTITADACGLQCPGPI"
     gene            2002..2718
                     /locus_tag="PKM80_04580"
     CDS             2002..2718
                     /locus_tag="PKM80_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV93219.1"
                     /translation="MDAQDKKIFIVEDERDILELIRMQLNSAGFTAKGFETALPMLNL
                     LKIEHPDLLILDLMLPDIDGMEVCRQLKNDKATQKIPILMLTARTDLEDKIKGLEYGA
                     DDYITKPFETRELIARIKAILRRSDWESDKNVLSITENFIIDFNRYEVWVQGKRLDLT
                     LTEFKILQLLTKRPGWVYNRSQILDYLWGNDKIVIERTIDVHIRNLREKLGNYAYLIK
                     NIRGVGYQFLSEIEDDIEKD"
     gene            2705..>3959
                     /locus_tag="PKM80_04585"
     CDS             2705..>3959
                     /locus_tag="PKM80_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV93220.1"
                     /translation="MKKTSITSLLIFAFAFLIFLGFILILTRVPIVLILIITIIIAAL
                     FVWILHIFLQKQFSLILQTTAQIVRGNHTIRIPELNTKEFSTLQEDLNRMLEKLDKTI
                     HHLAVHREELRLVLSSIDDILWSQNLEGKLEWINQSFQQLFGEINPKQQIYYWEVIRD
                     PVLLSFIKNSENSKDKLLKEAEIEGHYYLFSSTHNDVAKRRVFILQNIDEIRQVEQMK
                     KDFIVNLAHELRTPLTAISGFAEAMEDSINVTNRRYLHIIQNHTKRLIHLISDLEQLI
                     RLERSGSIELQEINLITFLNNISLILSPIIEEKGLYLKLELDDNLQRLVCDPFKLEQV
                     FINLVQNSLRYTTKGGITIKSKLIQKEAFIEVSDTGKGIDNIHLPRIFERFYVADPSR
                     NKTKSGTGLGLAIVKHIIQLHKGRKT"
BASE COUNT         1258 a          752 c          687 g         1262 t
ORIGIN      
        1 aattggtcct ggacattgta aaccgcaagc atctgcagtt attgtcttta tatctctatc
       61 cattttttct tccttgcctt tatagatatc atcgtcttta ccaatataat catccgcaaa
      121 gatatcctca ttactttgtt tctgagtagc gtattcataa gtaagatatc ctccggagag
      181 attataaaca ttcttaaacc cgttttgcat taaaatgcgc gcggcaaaat aagaacgcag
      241 accggttccg cagaaaacta ctacttgttt attttcaggt atttccttca accttgaccg
      301 taaatcatcc aaagggatgt ttatacttcc tgctattgtt cccaaagcag tttcatcagg
      361 agttcgcaca tcaatgataa tggtatcatt tttatccaag gaactcaaat catcccagtg
      421 aataatcttt actaatccgt tgatgatatt atcagctacc attccggcaa tgtttacagc
      481 atccttggcg ctggaaaaag gaggagcata actatgttca atttcttgaa tttcatagat
      541 ggtctcgtta tgtttcaata tatgagccaa catatctgtc cttttatcta cgccgtcaaa
      601 acctacaatt tgtgcaccca acagttttcc atctttaggg ttaaaaacaa tctttatcgt
      661 tatgggcagg gcatcagggt agtaacctgc atgagaagaa gtatgtataa tggaagagat
      721 ataatctatt cctgcatctt ttagaacttt ttccgataca ccggttgaac cgacagtaat
      781 atcaaaaact ttggcaatgg cagttccaat actaccttta tattttcgga tgttaccttt
      841 aacgatatta tcagccacaa tgcgtgcctg tttattggca ggtcccgcga gataggttaa
      901 gctttccatt ttagtaatcg gattgggata tgctattgca tcccccaaag cataaatatc
      961 aggatcactg gtttgcagat aggaatttac tcttatccct ttgtttattc ccaattccaa
     1021 accggctgct tctgccaatt tactatcagg acgaactcca atggataata ataccatatc
     1081 tgcatttagt gttcgcccgc tctgtaaatg gacttgtaaa ctgccatcat ttctactaaa
     1141 ggatgagaca ccgtcttgaa ggtaaaattc cacttctttc gtttttaagt gttgatggac
     1201 aatagccgcc atttcataat ccaaaggggt cataacctgt tccgccattt ctaccaaagt
     1261 tactttcatc cccaaattat gtaaattttc cgccatttcc aatcctataa aacccgcgcc
     1321 aataataacg caatgctgaa ctttatggct ttcaatgtat tccttaattt tatcagtatc
     1381 agaaacattt cgcagagtaa aaatagcgct atctgatatt ccaggtaggg gaggaattaa
     1441 aggttcagcg ccaggtgata gaaccagttt atcataattc tcattatatt ggctaccatc
     1501 tgctcttttt accgtaaccg ttttttcctt tctattgata gcaataactt cttggttgat
     1561 gcgaacatct acatttagtc gggctttaaa gctttcaggc gtctgaacaa acagccgatc
     1621 cctttcttta attacgcccc caatatagta tggcaaacca caatttgcat aggatatata
     1681 gctatagcgc tcaaacataa taatttccgc tttttcatcc aagcgtctta aacgagctgc
     1741 agttgttgct ccacctgcaa caccaccgac aatcaaatat ttggacatta ttataatctc
     1801 ctttttctat attatttact atattgaata agataattgt ttatgcatat ttggcaagta
     1861 tcaaatacat acatttcgtc tcataagatt ttgagattcc gttcaggaat agaaccatct
     1921 gaacgatttg ttaagaaaat ttaaccaatt attagtcctt ttttcttgct caaaattagt
     1981 taaattattt ataggttttt tatggatgca caagataaaa aaatatttat cgtagaggat
     2041 gaaagagaca ttctggaact tatcagaatg caactgaatt ccgcaggttt tacagcaaag
     2101 ggttttgaaa cagccctacc aatgttgaat ttgctaaaaa ttgaacatcc agatcttctt
     2161 attctggatt tgatgcttcc cgatattgat gggatggaag tttgtcgcca attgaagaat
     2221 gacaaagcaa ctcagaaaat accgatttta atgctcacgg caagaacaga ccttgaagac
     2281 aagatcaagg gtttggaata tggtgctgat gattatatca ctaagccctt tgaaacaaga
     2341 gaacttattg ccaggataaa agccatctta agacgcagcg attgggaaag cgacaaaaat
     2401 gttctttcca taactgaaaa cttcataatt gattttaacc gttacgaagt gtgggtacaa
     2461 ggaaagcgcc ttgatctcac tttaacggaa tttaaaatat tgcaactttt aactaaaaga
     2521 ccaggttggg tttataatcg cagtcagatt ttggattatc tttggggcaa tgataagata
     2581 gttattgagc gcacgataga tgtgcatatc cgcaatttaa gagaaaaatt gggtaattat
     2641 gcttatctga ttaaaaatat cagaggagtg ggttatcaat ttcttagcga gatagaagat
     2701 gacattgaaa aagactagta tcacttcttt gctgattttt gcttttgcct ttcttatatt
     2761 tctagggttt attttgattt tgaccagagt tccaattgta ctgatattaa ttataacaat
     2821 tattatagct gcactttttg tttggattct ccatattttt ttacaaaaac aattctcact
     2881 tatcttacaa acaactgcac aaatagtcag aggaaatcat accatacgca ttcctgaact
     2941 aaatacaaaa gaattcagta ctttgcaaga agatctgaac aggatgctgg aaaagctgga
     3001 taaaaccatt caccatttgg ctgtgcaccg tgaagaactg cgcttagttc taagctccat
     3061 agacgatatt ttatggtctc aaaacctgga aggcaaactg gaatggatta atcaatcctt
     3121 tcagcagctc tttggtgaaa ttaatccaaa gcagcaaatt tactattggg aagttattcg
     3181 cgatccagtg ttattatcct ttatcaaaaa tagcgaaaac agtaaagata aacttttaaa
     3241 ggaagcagaa atagaagggc attattatct attttccagc acccataatg atgttgccaa
     3301 acgacgggtt ttcattttgc agaatattga tgaaatccgt caggtggaac agatgaaaaa
     3361 ggatttcatc gttaatttgg cgcatgaatt acgcactccc ttaactgcga tcagtggttt
     3421 tgctgaagca atggaagata gcattaatgt aacaaacaga agatatttgc acatcattca
     3481 aaatcatact aagcggttaa ttcatttaat aagtgattta gaacagctaa tccgtctgga
     3541 acgaagcggt tcaatagaac ttcaagaaat caacttaatc accttcctga ataatatttc
     3601 gctgatttta tccccgatta tcgaagagaa agggctatat ttaaaacttg aactggatga
     3661 taatctacag cgtttagttt gtgacccctt taaactggaa caagttttta tcaatcttgt
     3721 ccagaattca ctgcgttata caaccaaagg aggcataacc atcaagagta aattaataca
     3781 gaaggaagca tttatagagg tctccgatac tggtaaagga attgataata tccatctacc
     3841 tcgtattttt gaacgctttt atgtagcaga tccctcccgg aataaaacca agagtggaac
     3901 tggactcggt ttggcaattg taaaacacat cattcaatta cataaaggca ggaaaactg
//
LOCUS       DAORHB010000131         3886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_25566_length_3886_cov_5.522318, whole genome shotgun sequence.
ACCESSION   DAORHB010000131 DAORHB010000000
VERSION     DAORHB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25566_length_3886_cov_5.522318"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKM80_04590"
     CDS             <1..561
                     /locus_tag="PKM80_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV93221.1"
                     /translation="PDDSIENIFAAAAEWEVGNAIERVKKAREIMLDRAEEASEYILK
                     YKLNTKSGLEYRALEVMVKENESFKQLLFNYTDDADSLKAKNALALIAGVGDSTLIEP
                     IRKHLNNGKYITTCLSLLGSIKSEESVNLLAEYAFHPCERYRYLVARSLKQINTPQAH
                     NYLIKMADDSSFLVKTLIRKFKEEQQ"
     gene            558..1199
                     /gene="rsmG"
                     /locus_tag="PKM80_04595"
     CDS             558..1199
                     /gene="rsmG"
                     /locus_tag="PKM80_04595"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNV93222.1"
                     /translation="MKDEKQIFEDYLNSLELENINQLMKRFEHYHNLLYTTNRKVNLV
                     SRNIPSDRYWVQHFLDSLVALECLDLEGKFVLDFGSGGGLPGIPLKLAVPDFNLTLLE
                     SVQKKSRVLREFVDALSLSATNVEASRLEDYAYVARRPSYDIIVCRAISLEERYISPI
                     RRLLKPSGMVVFYKAQKLDDVQNLHYEVLLDKNYPSLGQRKLIGIKQRDLMLR"
     gene            1223..1987
                     /locus_tag="PKM80_04600"
     CDS             1223..1987
                     /locus_tag="PKM80_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV93223.1"
                     /translation="MPNIITVVNQKGGVGKTTTAVNLAAALAVLEKRTLLVDFDPQGN
                     ATSGVGIDKDKVELQIYDALIDRAPIEKTILATSTENLYCIPGNINLTGAEIELVQEY
                     AREHKLKNALQPIVNSFEYIIIDCPPSLGLLTVNAMTAATEVLVPIQCEYYALEGVSQ
                     LLTTIRLIQKNLNPGLNILGVLLTMFDKRVNLSLQVAKEVHRYFKEKVFRTIIPRNVK
                     LTEAPGFGKPIFLYDIRSPGAMSYLNLANEVINRSK"
     gene            1998..2852
                     /locus_tag="PKM80_04605"
     CDS             1998..2852
                     /locus_tag="PKM80_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNV93224.1"
                     /translation="MNDRLGRGLSALIPENDLEENPKLGISTLPIDSIKPNRYQPRKI
                     FDAEKLAELTESIKENGIIQPLIVTKTTSSEYELIAGERRLEAAKLAGLEKVPVVIRS
                     VSKKEQLQFALIENIQREDLNPIEEALAYNALVEDFGLTHNQISEIVGKDRATITNSL
                     RLLKLPERIKQMLISGELNSGQARAVLSVKPELQISFAEYIIKYNLTVREAEDKAKTY
                     SEDKTAAPQKAKTSSRVVDLEKELHQLFKMKVKVQEANGKGKITFVYKSEEELLKFKT
                     ILDKIRQE"
     gene            2852..>3886
                     /gene="rfbB"
                     /locus_tag="PKM80_04610"
     CDS             2852..>3886
                     /gene="rfbB"
                     /locus_tag="PKM80_04610"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNV93225.1"
                     /translation="MKSVIVTGGAGFIGANYLHTLFADENFSGKILNIDKLTYAGNLD
                     SLQDLESKYGNSRYFFVRADICDAEKMKEVINQFKPDTIVHFAAESHVDRSIDGPMEF
                     VKTNLVGTATLLNAALEYYRTLQEAEKKNFRFHHISTDEVFGSLGESGMFTEETAYDP
                     SSPYSASKAGSDHLVRAWQRTFGLPITISNCSNNYGPYQFPEKLIPLMILNCLAHKPL
                     PVYGKGLNVRDWLFVTDHCEAINIIIRKGKFGETYNIGGHNEMKNIDIVTTICKILDE
                     LEPSPTLNSYTELITYVKDRPGHDLRYAIDAGKIEKDLRWKPAETFETGIRKTISWYL
                     ENREWWQNIQN"
BASE COUNT         1302 a          632 c          778 g         1174 t
ORIGIN      
        1 cctgatgatt ctatagaaaa tatttttgct gctgctgctg aatgggaagt tggtaatgcc
       61 attgaaagag taaaaaaagc cagagaaatt atgcttgaca gagctgaaga ggcaagcgaa
      121 tatattctta aatataaact taatacaaaa tccggattgg aatatcgcgc cttggaagtt
      181 atggttaaag aaaacgagag ttttaaacag ctactattta actatactga cgatgcggat
      241 agtttgaaag ctaaaaacgc tttggcatta atagcaggtg ttggagatag cactttaatt
      301 gaacctataa gaaaacacct gaataatgga aagtatatta ctacctgtct ttctctttta
      361 ggcagtatca aaagtgaaga gagcgttaat ttacttgctg aatatgcatt tcatccctgt
      421 gaacgttatc gctatttggt agcgcgttct ttgaaacaaa ttaatactcc tcaagctcat
      481 aactacctga taaagatggc tgatgacagt tcttttctgg taaaaaccct aattcgaaaa
      541 tttaaagagg aacaacaatg aaagacgaaa aacaaatctt tgaggattat cttaactcac
      601 ttgaactgga aaacataaac caattaatga aacgctttga acattatcat aatttgctgt
      661 atactactaa tcgtaaggtt aatctggttt cccgtaatat accttcggat agatattggg
      721 tgcagcattt tcttgacagc ttagttgctt tggaatgttt ggatttagaa ggaaaatttg
      781 ttttggattt cggttcggga ggtggtttac ctggaattcc tcttaaactt gccgttccag
      841 attttaattt aacacttctg gaatcggtac aaaagaaatc ccgggttttg cgtgaatttg
      901 tggatgccct atctctttca gcaacaaatg ttgaagcgag tcgtttagaa gattatgctt
      961 atgtggcaag gcgtccaagc tacgatataa tagtttgcag agccatttct ttggaggaac
     1021 gttatatttc tcccataagg agattgctga aaccaagcgg aatggttgtt ttttataaag
     1081 ctcagaaact ggatgatgtg caaaacctgc attatgaagt cctgttggat aaaaactatc
     1141 cttccttggg tcaacgcaaa ttaattggta ttaaacagcg agaccttatg ctgcgttaag
     1201 tttataaaat gaaaggatat atatgcccaa tattataact gtagtaaacc aaaaaggtgg
     1261 cgttggaaaa acaacaacag ccgtaaatct ggctgctgca ttggcagttt tagaaaaaag
     1321 gactctatta gtggattttg accctcaggg aaatgctacc agcggagttg gaatcgataa
     1381 agataaggta gaattgcaga tatatgatgc cttaatagat agagctccaa tagaaaaaac
     1441 aatcctagct acttccacag aaaacttata ttgcattcct ggaaatataa atttaacagg
     1501 agcggaaatt gaactggttc aagaatatgc gcgtgagcat aaattgaaaa atgccttaca
     1561 gccgatagta aattcattcg aatatattat aattgattgt cctccttcac ttggtttgtt
     1621 aactgttaat gctatgacag ctgctacgga agttttagtt cctattcaat gcgaatatta
     1681 tgctttggaa ggagttagcc aattacttac tactattcgt ttgattcaaa aaaacttaaa
     1741 tcccggatta aatattctgg gagtgctgtt aacaatgttt gataaaagag taaatctatc
     1801 tttgcaagta gctaaagaag ttcatcgcta ttttaaagag aaagtttttc gtacaattat
     1861 tccgcgcaat gttaaattaa cagaggcacc tggttttggg aaacctattt ttctttatga
     1921 tataagatct ccgggtgcga tgagttacct taatctggct aatgaagtta tcaatagaag
     1981 taaataggta gttatttatg aatgatcgtt taggacgagg tctttcagct ctaattccag
     2041 aaaatgatct tgaagaaaat cccaaattgg gtattagtac tctgccgatt gacagtataa
     2101 aacccaatcg ttatcaacca cggaaaatat tcgatgcaga aaagctggct gagcttactg
     2161 aatccatcaa agaaaatggc attattcaac ccttgattgt taccaagact acttcttcgg
     2221 aatatgaatt aatcgctgga gaaagaagat tggaagcagc caaattagct ggtctggaaa
     2281 aagtgccggt tgttattcgt agtgtttcga aaaaggaaca gcttcagttt gctttaattg
     2341 aaaatatcca acgggaagat ttgaatccta tcgaagaagc tcttgcctat aatgctttag
     2401 ttgaagattt tggattaact cataaccaaa tatctgaaat tgtaggaaaa gatagagcta
     2461 caataacaaa ttctctgcgt ttattgaaac taccggaaag aattaaacag atgcttattt
     2521 caggagaatt gaattcggga caggcacgcg ctgttctttc tgtgaaacct gaactgcaaa
     2581 ttagttttgc tgaatacatt attaaatata atttaactgt tcgtgaagct gaagataaag
     2641 cgaaaactta ttctgaagac aaaactgcag caccgcaaaa agcaaaaaca agttcccggg
     2701 ttgtagatct ggaaaaagaa ttgcatcagc tatttaagat gaaagttaag gttcaagaag
     2761 ctaatggtaa agggaaaata accttcgtct ataaatcgga ggaagaactt ttgaaattta
     2821 aaactatctt agataaaata aggcaggaat aatgaagagc gtgattgtta ccggaggcgc
     2881 aggttttatt ggggcaaact atttgcatac cctttttgcc gatgagaatt tttcagggaa
     2941 aatactaaat atagataaac ttacttatgc tggaaacttg gatagcttgc aggatttaga
     3001 gagtaaatat ggaaactctc gttatttctt tgtaagggca gatatctgcg atgctgaaaa
     3061 aatgaaggaa gtaataaatc agttcaaacc agatacaatt gtccattttg ctgccgaatc
     3121 acatgtagat cgcagtattg atggtccgat ggaatttgtt aaaacaaatc tggtgggaac
     3181 agctactttg ctaaatgctg ctttggaata ttatcgaact ttgcaggaag ccgaaaagaa
     3241 aaattttcga tttcatcata tttcgactga tgaagtattt ggctctttag gagaatcagg
     3301 tatgtttaca gaagagacag cttatgatcc ttcttctccc tattcagctt ccaaagcagg
     3361 ttccgatcat ttagtccgtg cctggcagag aacttttgga ctacctatta ctatttctaa
     3421 ttgttccaat aactatggtc cttatcaatt tcctgagaag ctgatccctt taatgattct
     3481 aaattgcttg gctcataaac ctttacctgt ttacggaaaa ggactaaatg tgcgcgattg
     3541 gcttttcgtt acagaccact gtgaagctat caatattatt atccgtaaag gtaaatttgg
     3601 cgaaacctac aatatcggag gccacaatga aatgaaaaac atcgatattg taactacgat
     3661 atgtaaaata ttagacgaac tggaaccttc acccactctg aatagttaca ctgaactaat
     3721 tacctatgtg aaagataggc ctggccatga tttacgctat gctattgacg ccggcaaaat
     3781 tgaaaaggac ttgagatgga aacctgctga aacttttgag actggcatca gaaaaaccat
     3841 ctcttggtat ctggaaaacc gtgagtggtg gcaaaatatt caaaat
//
LOCUS       DAORHB010000132         3866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_25777_length_3866_cov_3.494621, whole genome shotgun sequence.
ACCESSION   DAORHB010000132 DAORHB010000000
VERSION     DAORHB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25777_length_3866_cov_3.494621"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..773
                     /locus_tag="PKM80_04615"
     CDS             105..773
                     /locus_tag="PKM80_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNV93226.1"
                     /translation="MHNIKAVIFDMDGTLIDSMQVWRKVDNDFLHSRGLEVPIDLFEN
                     LPAGNSFIQTAQYFKDRFSLPDSPESIMQEWTKMVSHHYETDIKLKSGARELIVALKD
                     RGIKIGLGTSNSLELARKVLSHNAIWHYFQCAITGDLDLKGKPYPDIYLLAAKSLQEK
                     PENCLVVEDTLTGVQAAKAAGMKVFAVYDEDSYPYHSMMKEIVDGFYPDFHILATELF
                     RLRK"
     gene            840..1532
                     /locus_tag="PKM80_04620"
     CDS             840..1532
                     /locus_tag="PKM80_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93227.1"
                     /translation="MKPQLYIIIGAYGSGKSEYSIHLARSLKAQGLDVVLADLDVVNP
                     YFRSREIRDDFILEGIDVIAPEGEYKHSDLPMISPRVKGALQNREKTVILDVGGDPAG
                     CRTLGRFEEIIKNRGYNMIFIVNTMRPFTTTPEEIITMKEALEATSRLQITEIVCNTN
                     LMEETTADIVLSGIKTVDKAAKQLSLPFDTYLVLSKYISIVPDHLYGKKREVMKYNLQ
                     KPWELLIARGIC"
     gene            1709..3724
                     /locus_tag="PKM80_04625"
     CDS             1709..3724
                     /locus_tag="PKM80_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNV93228.1"
                     /translation="MQERSIKRIILVCSVLCLLAAAAFANNNTKEENIDRGIRFTETN
                     PPEAPVRPIAEFEPASAVLIRYPLGIPLSLVVQLANTANVICIVSSSQQNSAISAFTN
                     AGVNMDKVSFINTITDSYWTRDYGPWFIFDGNGEYGVVDFVYNRPRPNDNLVPQIFAN
                     QLNLNYYGMNLQQTGGNYMCDGVNTAAQTNLVYSENGNNQTNVNTKMNQYLGITNYCV
                     VADPNNTYIDHIDCWAKFLAPDKILIRSVPISHPQYNAIETTATYFANQNCAWGYPYR
                     VYRVNTPNNEPYTNSLILNKKVFVPIVGSSNDNPALQVYRTAMPGYEVIGISQASSTP
                     WESTDALHCRTHEIPDKNMLHIVHTPWHSIISAGTDIVFNVEITAHSGQPLYTDSLFV
                     CYKVNSGDWQRSYLQPLTRNNYTTSLSGFSYGDSIRYFIHAADQSGRNADHPVFASLD
                     PHLFVIQPDTTPPNLVHNPITTIGNQTEPISFILSTNDESGINQVLFRYHIDNSAISS
                     FPMDPISEDTYIFQYYPEFGAENRSFFYSFAAYDSVNPPNIGYLPGVDLWYEVPIRPV
                     AITDEVQTPSIMEGLISVYPNPFQAGKEVLNLRYFTEKQSSVTCKIYNIRGQIIYENT
                     SVPKASGLQELVWNGKDKQGYASPNGLYLVELIQGSNRYKAKLIIVK"
BASE COUNT         1238 a          677 c          733 g         1218 t
ORIGIN      
        1 cagttggtag agcgcaacct tgccaaggtt gaggtcgcgg gttcgaaccc cgtttcccgc
       61 tccagtttta tgctccaatt tattttataa ggaagtttca ttagatgcac aacattaaag
      121 cagtaatctt tgatatggat ggaactttaa ttgattctat gcaggtttgg cgtaaggtag
      181 ataatgattt tttgcattcc agggggctag aagttcctat tgaccttttc gagaatcttc
      241 ctgctggaaa cagttttatc caaacagccc agtattttaa agatagattt agcttacctg
      301 atagcccgga aagtattatg caggaatgga ctaagatggt tagccatcat tatgaaactg
      361 atatcaaatt gaaatcagga gccagggaat tgattgtagc attgaaagat aggggaatca
      421 aaatcggact gggaaccagc aattctttag aattggcaag aaaggtatta tctcataatg
      481 ctatttggca ttatttccaa tgtgccatta ctggcgatct agatctaaag ggaaagccct
      541 atccagatat atatttatta gctgcgaaaa gcttgcaaga aaaaccggaa aactgcttag
      601 tagttgaaga tactttaact ggtgtgcaag ctgcaaaagc tgcaggaatg aaagtttttg
      661 ccgtttatga tgaggatagc tatccttacc attcaatgat gaaagagatt gtagatggat
      721 tttatcctga ttttcacatt ttagccactg agctgtttcg ccttagaaaa tagatttatc
      781 atacctggtt tgaaattgaa caggtatatc ttttattagt gtatattagg agctcgttta
      841 tgaaaccaca attatatata attataggtg catatggaag cggaaaaagt gaatattcaa
      901 ttcatttggc acgcagttta aaagcacaag gccttgatgt tgttttagct gatttggatg
      961 ttgttaatcc ctatttccgt tccagagaaa ttagagatga ttttattctg gaaggtattg
     1021 atgttattgc tcctgaggga gaatataagc atagcgattt acctatgatt tctcctcgtg
     1081 tgaaaggagc cctccaaaat cgtgaaaaga cagttatttt ggatgttgga ggtgatcctg
     1141 caggttgtag aacattagga cggtttgaag aaattatcaa aaataggggc tataatatga
     1201 tctttatcgt taacacaatg cgtcctttta ctactacacc ggaagagata atcactatga
     1261 aagaagctct ggaagccact tcccgcttgc aaattacaga aattgtttgt aacacaaatt
     1321 taatggaaga aacaactgca gatattgttt taagcggaat taaaacagta gataaagcag
     1381 caaaacaatt aagcctccct tttgatactt atttggtact aagcaaatat atatcaatag
     1441 ttccagacca cctttatgga aaaaaacgcg aagttatgaa gtataattta caaaaaccct
     1501 gggaattgct tattgcaaga ggaatctgtt aatctgctaa ctctggatca tactatctca
     1561 ataatttatc tcgccttgct ttgtaataag ttctatatct ttcgaagatg gtaagaaatt
     1621 aagtagtaga cctttaaaaa aaaattgtgt tttggcacaa attatgtata ttattataat
     1681 atcactgtag aaacaggagt ttatcttgat gcaagaacga agtataaaac gcataatatt
     1741 agtatgcagt gtgttatgtt tattagctgc tgctgccttt gccaataata atacaaaaga
     1801 agagaacatt gataggggaa ttcgttttac cgaaactaat ccaccggaag cgccggttcg
     1861 tcccatagca gaatttgaac ctgcctcagc tgttctaatt cgttatcctc taggaattcc
     1921 tctttcatta gtggtacaac tggccaatac agcaaatgta atttgcatag ttagcagttc
     1981 tcaacaaaat tctgcaataa gtgcttttac taatgccggt gtaaatatgg ataaggttag
     2041 ttttattaat accataacgg attcttactg gacaagagat tacggaccct ggttcatttt
     2101 tgatggcaat ggagaatatg gagtggtaga ttttgtttat aatcgtccgc gtcctaacga
     2161 taatttagtt ccgcagatat ttgccaatca actgaattta aactactatg gaatgaattt
     2221 acaacaaaca ggtggcaatt atatgtgcga cggggttaat accgctgccc aaacaaattt
     2281 ggtctattca gaaaacggta acaaccaaac aaatgtaaac actaaaatga atcaatattt
     2341 aggcattact aactattgtg ttgtagcaga ccctaataat acatatatcg atcatattga
     2401 ctgctgggca aagttcctgg cacctgataa gatattgatt cgaagtgtgc caattagtca
     2461 tcctcagtat aatgccatcg aaactacagc cacctatttt gctaatcaaa attgtgcttg
     2521 gggttatccc tatcgagttt acagagttaa tactcccaac aatgaaccct atactaattc
     2581 cttaatttta aataaaaagg tctttgtccc tattgttggc agcagcaacg ataatcctgc
     2641 tttacaagtt tatcgtacag caatgcctgg atatgaagtt attggtatat cccaagcaag
     2701 ttctactccc tgggaatcta cagatgcctt gcattgcagg acacatgaaa ttcctgataa
     2761 aaatatgcta catattgttc ataccccttg gcatagtata atatctgcag gaacagatat
     2821 agtttttaat gtagaaataa cagctcatag cggtcaaccc ttatatacgg attcgctttt
     2881 tgtttgttat aaagtaaatt ctggtgattg gcaaagaagc tatcttcaac ctcttactcg
     2941 taataattat acaacatctt taagcggttt tagttatggt gattctattc gctattttat
     3001 tcacgcagca gatcaatctg gaaggaatgc agatcatcct gtttttgcaa gtttagaccc
     3061 acatcttttt gttattcaac cagatactac gcctcctaat ttggttcata accctattac
     3121 cacaatcggc aaccaaacag agccaattag tttcattctt tctactaacg acgaaagtgg
     3181 tatcaatcaa gttctattcc gttatcacat tgataattca gcaatttcgt ctttcccgat
     3241 ggacccaatt tctgaagata cttatatctt tcaatactac cctgaatttg gtgctgagaa
     3301 tagatctttc ttttacagtt ttgcagcgta cgatagtgtt aatcctccta atataggtta
     3361 tcttcctgga gtagatttat ggtatgaagt accaatcaga cctgttgcta ttaccgatga
     3421 agtgcagaca ccctctatta tggaaggttt aataagtgtt tatcccaatc cttttcaggc
     3481 aggcaaagaa gtgctgaatt taaggtattt tacagaaaaa caatcatccg ttacttgtaa
     3541 aatatataat atccggggac agattattta tgaaaatact tcggttccca aagctagcgg
     3601 ccttcaggaa ttggtatgga atggaaagga taaacaagga tatgcatcgc cgaacggttt
     3661 atatttagtt gagctcatac aaggttcaaa ccgctacaaa gcaaagctaa taatagttaa
     3721 atagagcgat gttgatatat ttcgcgtaaa aaatatagtg agtttgaatt ttcttcttga
     3781 caggaaaaca atatttaaat ataaagatct taagcctaca gaccctacat agaatgccct
     3841 ccgagcagtg gggatgccgc ccggca
//
LOCUS       DAORHB010000133         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_25950_length_3848_cov_3.942526, whole genome shotgun sequence.
ACCESSION   DAORHB010000133 DAORHB010000000
VERSION     DAORHB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25950_length_3848_cov_3.942526"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PKM80_04630"
     CDS             <1..444
                     /locus_tag="PKM80_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNV93229.1"
                     /translation="TVMVMVATLLVRIPVPGGGYFNLGDVVIIFSGLFGGWKAGLIAG
                     GIGSALADLIGFPVFAPITLVIKGLEGLVAGLAKPGIRWQRNLYPAIAVVIMVAGYFF
                     GCWLFPNLGLAVAFADLPVNIVQAAAGFFGGKALHIAYTKISKTS"
     gene            complement(1476..>3848)
                     /locus_tag="PKM80_04635"
     CDS             complement(1476..>3848)
                     /locus_tag="PKM80_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV93230.1"
                     /translation="LDHPEIDDLVADGLGGAIIKFRRFSSVNGINYLRRFANDGALIW
                     ATSYTLSNGEIRYPHQLFLNPNGILYDLGFAYRNDSIINIRTYNALAEQINQPLATYQ
                     LVLAGETNIENCNLKAVTTNGGDLQIGLLRNTINNDCEIFTYRFNQNLQPRYPVNGLV
                     LSNALYAIDNFDLAVNNDEVAFIWAEYNEATGSILKMERTNIQGSQYGYIYGYNLDQL
                     SGVYDSPQLLYQNNQMLVISKKTIDEYLKLSTDFYTITTKEGKDPQRTDLVSLINGSA
                     TLLANIPLADRSMLFYSDSRGGEYHLYCQGISLTGQLLFPENGKLINLGQLDYNSYKI
                     FKINSEQIGLLHYSNALYLQIISENGDFLLNGNGILISSAQLTRYNATCYEDDIYISW
                     VEPSSIYSNSCIYGQRIHNGTKMWGETGIMLKDLISSPYGYAFTGRYLLWKETVAGNN
                     TILCLKYDENGYPDADWNASGEIVINNLPTNQYSIVASYITGEDLLLLIAEYVIHPVF
                     FQKVTANHTLPWGDEGITFPATFFQLNYKYKADKLYLLYTARDSGSSSYNVFFQVIDS
                     GGNFLYPEMGLQVNTLAPGNDVSWSDSYANYPTLCVFDNNSCLTVWGSRMSRTDGQDL
                     YYRRINADGNFVESEDQLLCNAPYDQIYPLISTIGNQAIIGWQDRRMSHYTNCYGIYA
                     QRIDAYGSSLPPEITPEVVLFKSCYPNPFSKSTNIVWEQKNHSRVSINIYNLKGQLVK
                     SLTPSSMALGEHNIVWNSDDEKGKTVGNGIYFIRLQYEDKVITRKVMHLK"
BASE COUNT         1210 a          711 c          672 g         1255 t
ORIGIN      
        1 accgttatgg taatggttgc tacgctgctt gtgcgtattc ctgtcccggg tggaggatat
       61 tttaatttag gggatgttgt tattattttc agcggacttt ttggcggctg gaaagcagga
      121 ttaatcgcag gtggaattgg ttctgctctt gccgatctta tcggttttcc tgtttttgct
      181 cccataactc ttgttattaa aggtttagaa ggtttagtag caggacttgc aaaaccaggc
      241 ataagatggc aacggaattt atatcctgcc atcgcagttg tcattatggt tgcgggttat
      301 tttttcggtt gctggctttt ccccaatctt ggtcttgctg ttgcatttgc agatttaccg
      361 gtaaatattg tccaagctgc agctggattt tttggaggta aagcattgca tattgcttat
      421 accaaaatat caaaaacctc ataatgaacg gctaatcaca ctaatatcta taatattata
      481 gccacttgtg caataaggga aaatactatt ttccctttat tttgttcttt taccctatta
      541 ttatacgaga ccatagataa taattaccac tatgttctat ttcctatctg aactatttta
      601 gttctgcatc aaccaggttt tgagagcaat catacatttt tttccgtttc tcaatccttt
      661 taaagctgag gctcggtttg gattcaaaac gaaacatcat gaccaaaaaa ttcatcattt
      721 ggccattcag ttatgaaatt atagtaagta gccaatatga atttacacaa atatagatat
      781 caaggtaagt gctaattgaa tgtgcatctg caaaatggaa tgctaaaaaa cctctgaaac
      841 atatatgctt aaatagcatt tgctgaatga gaatttggca acttagggac tatccgaata
      901 gactgggaat tggcattatt accaattgag ctgaaattgc ttctttattg tagaaactaa
      961 attaatgctt gcttaatgaa ctgtggtaga gataccgtgg tgctactcgg caaaaaccca
     1021 ggaggctcca ttgtaactcc acagtaactc attaaggaag aatatggttt tttaaataag
     1081 cagaagggat taccaaatca ctttatctat cattctttct gcaatattca tagtttagca
     1141 gttgaacaat tagatttctt gttttatggt tttctataaa acgattctgt atgctatggc
     1201 aacgaggttg aaagagcaaa gacataattt ctactctgtt cgttcttttt tttagctgag
     1261 gctcggtttc gaatttccaa ggaacagcaa ggcgaggaac aaaatcacac taacttgttg
     1321 ctatttatgc atatttgcgg aatccaataa cgattaggtt gttactacct aaaatcaagg
     1381 atcgcaatag caatcagaca aatccaaggc aaaatgccat tttcgtctga tgttgaggcc
     1441 tatttcatta tatggctatc aattcggata gggaattact tcaggtgcat tactttacgg
     1501 gttataactt tatcctcata ttgcagacga ataaaataga taccgttgcc aactgtcttt
     1561 cctttttcat catcagaatt ccaaactata ttatgctccc ctaaagccat ggaacttggt
     1621 gttagagatt taacaagctg ccctttgagg ttgtatatat ttatactaac tcgagaatgg
     1681 ttcttttgtt cccaaacgat attagtgctt ttagaaaaag gattgggata gcaagatttg
     1741 aatagaacaa cttccggagt aatctcaggt ggcagagaag aaccgtaagc atcgattctt
     1801 tgcgcataaa ttccatagca attagtataa tgactcattc ttctatcctg ccaaccgatt
     1861 attgcctgat taccaatagt ggatattaat ggatatatct gatcatacgg tgcattgcaa
     1921 agtaattgat cctcactttc cacaaaatta ccatctgcat taattctgcg gtaataaaga
     1981 tcttgtccat ccgttctcga cattctggaa ccccaaacag ttaaacaaga attattatca
     2041 aaaacgcaaa gagtaggata attagcataa gaatcactcc aagagacatc attaccaggt
     2101 gctaaagtat taacttgcaa tcccatttct ggatacagaa aattgccacc actgtcaatt
     2161 acctggaaga agacattata tgaactactg ccactatctc tagcagtgta gagaagataa
     2221 agtttatctg ctttatattt ataatttagc tgaaaaaatg ttgcaggaaa agttatccct
     2281 tcatctcccc aaggcaaagt atgattggca gttacttttt ggaagaaaac tggatgaata
     2341 acatattctg caatcagcaa caagagatcc tcacctgtta tataactggc aacaatagaa
     2401 tattgatttg tgggcaaatt attaataact atttctccgg aagcattcca gtctgcatca
     2461 ggatagccat tttcatcata tttcaaacat aggatagtat tattgccagc taccgtctct
     2521 ttccataata gatatcttcc tgtaaaagca taaccatagg gtgagctaat caagtccttc
     2581 aacataatac ctgtttcacc ccacatttta gtgccgttat gaattctttg tccatatatg
     2641 caactattgg aatatataga acttggctca acccaactga tatatatatc atcttcataa
     2701 caggttgcat tataacgagt taattgagcc gatgaaataa gaatgccatt cccatttaat
     2761 aaaaaatctc cgttttcgga tataatctgc agatagagag cattggaata gtgaagcaac
     2821 cctatttgtt cagaatttat tttaaagatt ttataggaat tatagtccaa ctgaccaaga
     2881 ttgatcagtt ttccgttttc aggaaataac aattgtccag ttaaagatat accctgacag
     2941 tataaatgat attctccccc tctggaatca gaataaaaca acatagatcg atctgctaat
     3001 gggatatttg ctaaaagagt agcactaccg ttaataagag aaacaagatc agtccgctga
     3061 gggtctttgc cttcttttgt cgttatagta taaaaatcgg tgcttaattt cagatattca
     3121 tctattgtct ttttgctgat aactagcatt tggttgtttt gatataataa ctgaggtgaa
     3181 tcataaacac cgctcaactg gtctaaatta taaccatata tatagccata ctggcttcct
     3241 tgaatattgg ttctttccat tttcagaatg ctaccggttg cttcgttata ttcagcccag
     3301 ataaaagcca cttcatcatt gtttacagcc agatcaaaat tgtcaatagc gtaaagggcg
     3361 ttggaaagta caagtccatt gaccggataa cgaggctgca aattttgatt gaacctgtaa
     3421 gtaaagatct cgcaatcgtt atttatcgta ttacgcaata atccgatttg cagatctcca
     3481 ccattggtag taactgcttt caaattacaa ttctcaatat tagtctctcc agcaagaaca
     3541 agttgataag ttgccagagg ttgatttatc tgctctgcta aagcattata agttctgata
     3601 ttgatgatgg aatcatttct ataagcaaaa cccagatcat ataaaatacc attgggattt
     3661 aaaaataact gatgtgggta tcttatttct ccgttgctta aagtatagct tgtcgcccaa
     3721 attaatgcac catcattggc aaagcggcgt aaataattaa ttccattaac actactgaat
     3781 ctgcggaatt tgattattgc tccacctaaa ccatctgcaa ccaaatcatc aatttcagga
     3841 tgatccaa
//
LOCUS       DAORHB010000134         3844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_25977_length_3844_cov_5.413566, whole genome shotgun sequence.
ACCESSION   DAORHB010000134 DAORHB010000000
VERSION     DAORHB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25977_length_3844_cov_5.413566"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..2947)
                     /locus_tag="PKM80_04640"
     CDS             complement(224..2947)
                     /locus_tag="PKM80_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNV93231.1"
                     /translation="MKRYLIYALLFFPALILANSYSVQIQFPFPREGFSSSALYEQGY
                     GFYIAPGALQLPVKQINILLPKDAIVNDWQLTFDPDITFKGEAPIFNSAFTNGEEILG
                     APIKRNNPSRYNFLGLRKWGDLNYASFYVIPGIYDGSNWLWNSSCRIDINYSASAKQN
                     GKIPPTFQKRDFFANPQCLINWYEASKEPNNRLLVITTPSLYTALNDLVLFRQTQGID
                     VNFCDIATALASGTGTDNAEKLRNYLQNTYNQNPFSYLLLVGDDVTIPTAYVTPEPNG
                     LETVATDFFYSDLSSNWDTDSDGLRGEYSTGYLNEDYGIDFTSEVFVGRISTNYASEV
                     SAIANRIVAYEQSTEPWKDKNLLSAAFLNYQGEPEIIYPQTDGGLFMEFLRNTCLVGQ
                     DNFTMYEQEGVIPSFPSDLPANYNNLLNALNNESWGFINWSAHGSAIHSSRKVWSIDY
                     NNNNIPDTYEMNWMTLVDCQSFDDLENQDGTVIFAASCYNGYMDYYDTCLSEYALIKK
                     AVGVLAATRTGWYKIGWLNPGWGGLSSYNYHFVENFRNNKLDLGSAHSWANLLHTQYY
                     LFGDPIDSEGIIYPELQNVYTYMLFGDPMIGWALEETDLDGEILVWEPEGNAGLEVVN
                     ALREIRNFNVIYTDKLIPEYSYLHNFEAVIYLAANSVASSSLDPDSYEYEYLNSYLDS
                     GGKLYCENYIDFSFGELYNKMGAQLDNPVNLNTIYHPESNISWNYSNADSTVYALIPI
                     QPNAQGIFISGDGETDNSIVGILNSTEDYSVITSSFQLSRILPGEHNLPDLMEIILAE
                     LNVTNSNPDSNNEQIICPTVQSVSVYPNPASQNSQLHFQMRKATPISISIFNIKGQKV
                     KTLINNELKEGEYHLSWDGKDNNGRTCSSGVYYYQIAAPNQNITKKMLLLK"
     gene            complement(2980..3738)
                     /locus_tag="PKM80_04645"
     CDS             complement(2980..3738)
                     /locus_tag="PKM80_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV93232.1"
                     /translation="MNYNYDHSRIVVILVEPIYGGNVGAIARIMNNFCFSQLRIIGKT
                     PEKNDFYLAMHSEHILENVCIYSSLAEAVKDLDRVIAFSRRIGKNKPVDLSPRQMARY
                     VHSVPNAKIGLVFGRETYGLTDSEAELCPLRCHFMANPDFPSINLAQAVALALWEIYS
                     LPLEEKQGENKYYNAASKEELDEIHHYIIQVMHSTGFFQKQETVNWNAFIAKMLNELN
                     PDKTMLYRLRQIFNRFNVLVTGKGLGYSISNDKE"
BASE COUNT         1157 a          736 c          710 g         1241 t
ORIGIN      
        1 cttatttcgg ttagtaattc gaattatggc agctgtgata aaaaaatcac tcatcagcca
       61 tatatgccag agtatgcaat gtactttttt tattatcata gcttagtttt ccatctggtc
      121 tgcatcctat ccttaggcat tttatctatt taaggttatt ttatttgttc atcctgggta
      181 ttaatgaaac tctttatact cccaaatctt aatactccaa gctctatttt agcagcagca
      241 tttttttggt aatattctga ttgggggcag ctatttgata ataataaact ccactggaac
      301 aagttcttcc gttattatct tttccatccc aggataaatg gtattcgcct tcctttaatt
      361 cattattaat aagggttttt accttttgtc ctttaatatt gaagatagaa atggatattg
      421 gtgttgcttt tctcatttga aagtgaagct gtgaattttg tgatgccgga ttgggataaa
      481 cgcttactga ttgtactgtt ggacagataa tctgttcgtt attgctatcg ggattactat
      541 ttgtaacatt tagttcagct agaataatct ccattaaatc tggtaaatta tgttcacctg
      601 gtagaatacg agataattga aacgaagagg taataaccga gtaatcctct gtcgaattca
      661 aaatgccaac aatggaatta tcggtttctc cgtctcctga tatgaatatt ccctgagcgt
      721 taggttgaat aggtattaaa gcgtaaacgg ttgaatcggc attgctataa ttccagctaa
      781 tattgctttc gggatggtaa attgtattaa gattaactgg attatccagt tgagcgccca
      841 ttttattata tagttctcca aaactaaaat ctatatagtt ttcgcagtat agtttaccgc
      901 cagaatctag gtagctattt agatattcgt attcatagga atcgggatcg agggaagaac
      961 tggcaacgga attagctgcc agatagataa cagcttcaaa attgtgcaga tagctatatt
     1021 cagggatgag tttatcggtg taaattacat tgaaatttct aatttctctt agtgcattaa
     1081 caacttccaa tcctgcattt ccttctggtt cccacaccaa aatttcacca tccaaatctg
     1141 tttcttctaa agcccaccca atcatcggat cgccaaaaag catataggta tatacatttt
     1201 gcaattcggg atagattatc ccttcgctat ctataggatc accaaaaaga taatattgag
     1261 tatgcagaag gtttgcccaa gaatgtgcag aaccaagatc aagcttgtta ttacgaaagt
     1321 tttctacaaa atgatagtta taactggaaa gtccgcccca accaggattc agccatccta
     1381 ttttatacca tccggttctg gtggcagcta aaacacccac agcttttttt attaaggcat
     1441 attcggacaa gcaggtatca taataatcca tatatccgtt gtaacaggaa gcggcaaata
     1501 taacggttcc atcctggttt tccaaatcat cgaaactttg gcaatctacc aaggtcatcc
     1561 aattcatttc ataggtatct ggaatgttgt tgttattata gtctatcgac catacttttc
     1621 gggaagaatg gattgcgcta ccgtgtgcgc tccaatttat aaagccccag ctttcattat
     1681 taagtgcatt tagaagattg ttataatttg caggtaagtc actgggaaat gatggtatta
     1741 caccttcttg ctcatacatt gtgaaattgt cttgtccaac caaacaggtg ttgcgtagaa
     1801 attccataaa aagtccgcca tcggtctgtg gataaattat ctctggttcg ccttgatagt
     1861 ttaagaaggc agcagatagc agatttttat ctttccaagg ctcggtagat tgttcatatg
     1921 caacaatgcg attggcaata gcagatacct ctgaagcata attagtagaa attctcccta
     1981 caaatacttc ggaagtaaaa tcaataccat agtcctcatt caaataacca gtagaatatt
     2041 cccccctcaa accatcgcta tcggtatccc aattactgct gaggtctgaa taaaagaaat
     2101 ctgttgcaac agtttccaat ccattgggtt cgggggttac ataagccgta ggaattgtta
     2161 catcatctcc aactaatagc aaataggaaa aaggattctg attataggta ttttgcagat
     2221 agttgcgtaa tttttcagca ttatcagttc ccgttcccga agccaaagct gttgctatat
     2281 cacaaaaatt aacatcaatt ccttgtgtct ggcgaaataa aacaagatca ttcaaagcag
     2341 tataaagcga gggagttgta ataactaaaa gacggttatt cggttcctta gaggcttcat
     2401 accagtttat gagacattgg ggattggcaa agaaatccct tttttggaaa gtgggtggaa
     2461 ttttcccatt ttgtttggca gaagcagagt agtttatatc tatccggcaa gaggagttcc
     2521 acaaccagtt tgaaccatca tatataccgg gaataacata aaaactggca taattcagat
     2581 ccccccattt tcttaaccca agaaagttgt aacgcgaggg attgtttctc ttaatcggag
     2641 cgcctaatat ttcttctcca ttagtaaaag cggaattaaa tattggggct tctcccttga
     2701 aggttatatc tggatcaaaa gtaagctgcc aatcattcac tatagcatct ttgggtaata
     2761 gaatattgat ctgtttaaca ggtagctgta aagctccagg agcaatgtaa aagccataac
     2821 cctgttcata taaagctgac gaagagaatc cttcccttgg aaaaggaaat tgaatctgaa
     2881 cgctatacga attagctaaa attagcgcag gaaagaatag taaggcatat attaggtatc
     2941 gcttcatttt ttttacctca accttatata gagcattttt tattccttgt cattggaaat
     3001 gctgtaaccc aggcccttgc cagttactaa aacattaaaa cgattgaaaa tttgccgcag
     3061 acggtaaagc atagttttat caggattaag ttcattaagc atcttcgcta taaaggcatt
     3121 ccaattaact gtttcctgtt tctgaaaaaa acctgtggag tgcatcactt ggatgatgta
     3181 gtgatggatt tcatccagtt cttctttact tgctgcatta tagtatttat tttcaccttg
     3241 cttttcttcc agcggaagac tgtaaatttc ccaaagagca agagcaacag cctgagctaa
     3301 gtttatcgag ggaaaatcgg gattagccat aaaatgacag cgaagcggac aaagctctgc
     3361 ctcagaatct gttaagccat aagtttcgcg tccaaaaacc agcccgattt tagcattggg
     3421 cacactgtga acatatcttg ccatctgcct tggactaaga tctacaggtt tgttttttcc
     3481 aatgcgtcga gaaaaggcaa taactctatc cagatcttta actgcctcag ccagagaaga
     3541 ataaatgcag acattttcca atatatgttc gctatgcatt gctaggtaaa aatcgttctt
     3601 ttctggggtt tttccgatta tgcgaagctg cgaaaaacaa aaattattca taattcgagc
     3661 aatcgctcct acattcccgc cgtagattgg ttctaccaaa attaccacta tccgagagtg
     3721 atcatagttg taattcatcg ttacctggat ggatggttgt ttcctgaatt agccaccttt
     3781 tggttttgtt gtgttttctt ccttttcttg tttattatcc ccgaaagctt tgaacccatc
     3841 atag
//
LOCUS       DAORHB010000135         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_26548_length_3787_cov_5.283226, whole genome shotgun sequence.
ACCESSION   DAORHB010000135 DAORHB010000000
VERSION     DAORHB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26548_length_3787_cov_5.283226"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PKM80_04650"
     CDS             complement(<1..472)
                     /locus_tag="PKM80_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNV93233.1"
                     /translation="MNAYYRQPAIYKDQVVFIAEDDLWTFSLKDEVARRLTANLGMIR
                     NPQFSPDGKQIAFTSTEEGTSEIYIMPSEGGEAKRLTYLGAYLYTLGWKGSKIIFASS
                     HSSYLQGYYKLYEIDCEGGAPVELPYGRATDISFGEKGVVLGRNTGNPARWKRYR"
     gene            complement(909..>3787)
                     /locus_tag="PKM80_04655"
     CDS             complement(909..>3787)
                     /locus_tag="PKM80_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV93234.1"
                     /translation="IGNGVNVAARLMTSAKYGQIVFTKDILPSVQSRFEVQFLRKDKV
                     KGIKGELEFYHIVRELPEYLNSYKKQYDDKTQVFYQNETESIIKKIASKKLSQLLISG
                     EHGTGKSFISWKIISSFYAKASKIAIIVLDEFNRHDFLILHRKFISKSLGIDDPLAEP
                     DKLKEYLYNLMDKRDSDILLQALGLQNQGSKQIIDSEKHKDLVLLSLYKSLYILMKDL
                     DIVLLDNIQWLDDLSNQIIQKRLEEETIITPILIMTSTDDFKHNKSNRSAKTAYLKLD
                     NLDYQSSFELIRSQLSNITHQAVDYILNTAGGNPRFIVELCAQIRSQFPNPAMLITES
                     DIRDIQNRGLLPLSVENLFMIKYDSLSADAKDMLKKASIIGKGFTLDEIVETQKSINQ
                     NEIVPIINELQKKEIIDITELSPEVQYLFNNVLMRQAIYSTILLSEKVSLHNRVALFY
                     EEKYAGWTSQHSELLAYHFHLGENKEKALHYSLLAAEQNYKISNYGEAIYYYQIALQN
                     TDDSEQLNSIILAIIDSQLYLGEVDKAKMYLENIQVLEIRNADEISKYHFLRLRIYYL
                     EGNYEEVLNYLSQINFYTGIYGDQIQLYQLDCLYKLFRIDEFSTLMKKLKQEYLKQAA
                     TLLKDKQQKTTLTSLLNIYKQIPDEKTTAEQRHYLYLLLKLEAIQANHFLNTGQYKEA
                     VTSLKFQYNLAKALKDDLSMRIACGGLGIVYTRMGKLDEAHQAYIEAINIADKISDRF
                     GYAKVLADMATLHRRMGNHKQALDNFQRSLKIFESLGNLVLQGFVLHGIGEVLIQEEK
                     YDESLVYFRKALKIAKQCNDLFGISFEQDAIGDILFQNGKITEAKTLYHKNLKLQKKI
                     GDTEGIAHTYGNLGNVARAENNLPLAMEYYNKNIAMTAEIGDLDGNGRGYYNLAGIYE
                     IMKDKDKFVELLNKSIECFQKAGSVQYLELAKQRLQEFERGSEV"
BASE COUNT         1130 a          695 c          628 g         1334 t
ORIGIN      
        1 cccgatatct tttccacctt gcaggattgc ctgtatttct acctaaaaca actcctttct
       61 ctccaaaaga aatatcggtt gctcttccat aaggtaattc aaccggagct ccgccttcac
      121 aatcaatttc gtaaagtttg taataacctt ggagataaga actgtgagaa gaagcgaaaa
      181 taatcttgct gcctttccat ccaagtgtat aaagataagc tcccaaataa gttaagcgtt
      241 ttgcttcgcc accctcggaa ggcattatat aaatttcgga agttccttct tctgtactgg
      301 taaacgcaat ttgcttacca tcaggagaaa attggggatt gcgaatcata cctaaattgg
      361 cagttaagcg cctggcaact tcatctttta agctaaaagt ccagaggtca tcttcagcaa
      421 taaagaccac ttgatcttta tagatagcag gttgacgata atacgcgttc ataaggatgc
      481 tcctttaata ataacttact ttattatttt gcactctttt catagtggat atttaagtca
      541 agtaaatata taataaggtt aatctaaaaa aagtatcaaa aataaggaca tcgaagaaac
      601 taaaatcgtt tctgccaaag taactttcac gcattcctat aatttgttta gtatagatgg
      661 ctcataacga ttgtaattaa taaataaaac acataaagct gtcataaagt tgggggtttt
      721 tcaagttata ggcaaagaca aagttatcaa aacgctttct gatttacaag aagaacgata
      781 tcttcttcgt ttcctggagg cagagttata aagtttccga gatcttccaa ctaatttcac
      841 gagtattttt tgggtggtta acgaggaaga aaattaccag caagatttac atccagatcc
      901 caaatccttt agacttcaga ccccctttca aattcctgca gtctctgttt agccaattcc
      961 agatattgta ccgaacctgc tttttgaaag cactctatgg atttgttcaa taattctacg
     1021 aatttatctt tatccttcat aatttcatag atacctgcta gattatagta gccccttcca
     1081 tttccgtcca gatctcctat ttcagctgtc atagctatat ttttgttata atattccata
     1141 gcgagaggaa ggttattttc tgctctggca acatttccta ggtttccata ggtatgagcg
     1201 atcccttccg tatcgccaat ttttttttgt aacttcaaat ttttgtgata gagtgttttt
     1261 gcttccgtaa tttttccatt ctgaaacaga atgtcgccaa tagcatcctg ttcaaatgaa
     1321 ataccaaaga ggtcattgca ctgtttagct attttcagtg ctttgcgaaa atatactagg
     1381 gattcatcat atttttcttc ttgaataaga acttcgccaa ttccgtgtaa cacaaaacct
     1441 tgcaacacaa gatttcccag agattcaaat atcttcaagc tgcgctggaa attatccaat
     1501 gcctgtttat gatttcccat tcttctatgc aaagttgcca tatcggcaag tacttttgca
     1561 tagccaaagc gatcactgat tttatcggca atattgattg cttcaatata tgcctgatgt
     1621 gcctcatcta attttcccat tctggtataa acaattccta aaccaccaca ggcgatacgc
     1681 atagaaagat catccttcag agcttttgcc agattatatt gaaatttaag cgaagtaact
     1741 gcttctttat actgtcctgt gttcagaaaa tggtttgctt ggattgcttc cagttttagc
     1801 aagagatata gatagtgtct ttgttctgct gttgtttttt catcagggat ttgtttataa
     1861 atatttaata gagatgtaag tgtagttttt tgttgcttat ccttcagaag tgtagcagct
     1921 tgctttaggt attcttgttt taattttttc atcaaagtag aaaattcatc tatgcgaaac
     1981 agtttataaa gacaatctag ctgataaagt tgtatctgat ctccatatat tcctgtatag
     2041 aagtttattt gagatagata attcagcact tcttcataat ttccttccaa gtaataaatt
     2101 cttaaacgga gaaaatgata tttacttatt tcgtcagcat tcctaatctc caacacttga
     2161 atgttttcca gatacatttt tgccttatcc acttctccca gataaagctg agaatctata
     2221 atagccaaaa taatggaatt cagttgttca gaatcatctg tattttgcag agctatttga
     2281 taatagtaga ttgcctctcc atagttacta attttataat tctgttcagc tgccagcagt
     2341 gaataatgca gtgctttttc tttattttca cctaaatgaa aatggtaggc aagcagctca
     2401 ctgtgttgag aagtccagcc tgcgtatttt tcttcataaa ataaagctac cctattatgt
     2461 agcgacacct tttcacttaa tagaattgta gaataaatag cttgcctcat caatacatta
     2521 ttaaaaagat attgaacttc gggagatagt tccgtgatat cgattatttc ttttttctga
     2581 agttcattta taatagggac tatttcattt tggttgatgc ttttctgagt ttccactatt
     2641 tcatccaaag tgaaaccttt tcctataata gatgcctttt ttagcatatc tttggcgtct
     2701 gcactaagtg aatcatattt aatcataaac agattttcta cacttaaggg aagtaaccct
     2761 ctattttgaa tatcacgaat atcagattct gtaattagca ttgctggatt tggaaattga
     2821 gaacgaattt gagcacaaag ttccacaata aaacgcggat ttcctccagc cgtatttagg
     2881 atgtaatcaa ctgcctgatg tgtgatatta gataattgcg aacgaattag ctcaaaactg
     2941 ctttggtaat ctaaattgtc cagttttagg tatgcagttt tggctgatct attacttttg
     3001 ttatgtttaa aatcatctgt tgatgtcatt atcaggatag gggttataat tgtttcctct
     3061 tccaggcgtt tttgtatgat ttgattagaa agatcatcca gccattgaat gttatccaaa
     3121 aggacgatat caagatcttt catcaagatg tatagtgatt tatacagaga aagcagcacg
     3181 aggtctttgt gtttttcact atcaataatt tgcttagagc cttgattttg taaaccgagc
     3241 gcttgtaaca ggatatcaga atcgcgctta tccattaggt tatatagata ttctttcaat
     3301 ttatcaggtt cagcaagtgg atcatcaata cctaagcttt tactgatgaa ctttctgtgt
     3361 aaaatgagaa aatcgtgacg gttgaattca tccaaaacga ttattgcaat tttgctggct
     3421 ttagcgtaaa aactgctgat gattttccag cttataaaag atttacctgt tccgtgctct
     3481 cctgagatta gcaattgaga gagctttttg gaagcgatct ttttgataat gctttcggtc
     3541 tcattttgat aaaatacctg agttttatcg tcatattgct ttttatagct gtttagatat
     3601 tcaggtaatt cccgcacaat atgatagaat tctagttccc ctttaatgcc cttaacttta
     3661 tcctttcgta aaaattgaac ttcaaaacgg ctttgtacac tggggagaat atcttttgta
     3721 aacacaattt gaccatactt tgcagaagtc atcaagcgag cggcaacatt aactccattg
     3781 ccgatta
//
LOCUS       DAORHB010000136         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_26604_length_3782_cov_3.764690, whole genome shotgun sequence.
ACCESSION   DAORHB010000136 DAORHB010000000
VERSION     DAORHB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26604_length_3782_cov_3.764690"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKM80_04660"
     CDS             complement(<1..410)
                     /locus_tag="PKM80_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV93235.1"
                     /translation="MLLQKYIESRLDMIETSLKEYLGTQSGPNKQLKQAMHYAVFSGG
                     KRWRPLLLLSIYEMLMRMRKNTGLPQALKAATAVELVHNASLVHDDLPIIMNRTERRS
                     QPALHTKFGNATAILTGDALYTLAFEVLGQISPPS"
     gene            complement(566..1594)
                     /locus_tag="PKM80_04665"
     CDS             complement(566..1594)
                     /locus_tag="PKM80_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV93236.1"
                     /translation="MEKLRLGLIGCGRISKNHFEAISQIPEAEYVAVADIIRERAEEA
                     AKTYNIPSVYTDYHKMLDKEQLDAVSICTPSGLHPSIGIDVANHKINVITEKPMAINV
                     EGADKLIHACDENNVQLFVVKQNRLNTTMQLLKRAVDKGRFGRIYMAQSNVFWQRPQS
                     YYDAAKWRGTWEFDGGAFLNQASHYVDALYWLLGNVGSVMAYTATMARHIEAEDTGCA
                     ILHFRSGIIATLNVTMLTYPKNFEGSITIIGEKGTVKVGGVAVNKIEKWEFEEYDDDD
                     RIVEEANYQPPNVYGFGHNPYYRNVVDVLLKRAEPLTDGRDGRKSVEIVQAIYLSAKT
                     GKRISLPL"
     gene            complement(1595..2599)
                     /locus_tag="PKM80_04670"
     CDS             complement(1595..2599)
                     /locus_tag="PKM80_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV93237.1"
                     /translation="MNILVTGSNGFVGSRLMWELEKLNHKVIGIDISSHCDAKPHPET
                     VIGDIRSSKDLEKVQDIFYQKYNHQIELIIHCAASKHDFGISREEYFSHNQYGTQVLL
                     EFAEQADIHKIIYISTVSVFGHPQGRADEDTPYNPDHPYGESKLGGELLCIEWQKKNP
                     ENELIVLRPTAIYGPHNFANIYKLIDTLHRTPLFTVGKGDYVKSIVSLDTVIDMILFS
                     MSLLKPGYQHFNCVDEPYITLNELMHIIAANPGFHMPKITIPDWLAIFIGKLFDIPAK
                     LFSIDIPINSDRMRKLSMATNFCSQKIRQAGFVQRKSIEESISEMCTWYLDYIENKEK
                     "
     gene            complement(2783..>3489)
                     /locus_tag="PKM80_04675"
     CDS             complement(2783..>3489)
                     /locus_tag="PKM80_04675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV93238.1"
                     /translation="GCIPLLQGPGFTLRPDKITIIPLGFDFSRLLPYDNECKLRKKLN
                     IAKDKITIALIGRVTAIKNPALFIYSANEVLKQRNNVHFLIIGDGELTDDCKKLVNQL
                     GIQDYFTFPGFIEDLKLIYGSVDIVCLTSINEGTPVSLLEAMASQKIVIATPVGGVPD
                     FVKNGENGFLCDANPQAIAEQIIYCVDNFQNLDYLRKQAAEEVLSKYSMNRLFKDLEN
                     LYNSTPSKKSPNLFND"
     assembly_gap    3490..3499
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3500..>3782)
                     /locus_tag="PKM80_04680"
     CDS             complement(<3500..>3782)
                     /locus_tag="PKM80_04680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV93239.1"
                     /translation="DFLAEIQLIKILKKEKPDIVHTHTSKAGTLGRLAAILTGVPQIY
                     HTFHGHTFEGYFSPLKTKFFVQIERFLARFSTKIIAISEQQKEDLIRFKI"
BASE COUNT         1194 a          707 c          628 g         1243 t
ORIGIN      
        1 gaaggaggag aaatttgtcc taaaacctca aaagcaaggg tgtaaagtgc atcaccagta
       61 agaatagcag tagcatttcc aaatttggta tgcaacgcag gttgagagcg ccgctctgtt
      121 ctattcatta ttataggtaa atcatcatgc accagtgaag cattatgaac cagttccaca
      181 gcagtggctg cttttaaagc ctggggtaaa ccagtatttt ttcgcattct cattaacatt
      241 tcatatatag aaagcaaaag caaaggacgc cagcgctttc cgccagaaaa aacagcataa
      301 tgcatagctt gttttaactg tttattcggt ccagattgag ttcccagata ttccttcaag
      361 ctggtttcaa tcatatctaa tctactttca atatattttt gtaatagcaa gttattctcc
      421 tgaaggctgt tactttgatt tgtctattat tataaattca ttaaacgata aagggtatta
      481 ttgtcaagtt ttttgttccg gttttaatta aaaaattagg gagattgtaa aatctccctg
      541 ttttgctata tataaaatga ttggattata aaggtaggct tattctctta cctgttttag
      601 ctgaaagata gatagcttga acaatttcaa cggacttgcg tccatcacga ccatcggtta
      661 gaggttctgc tctttttaga agaacatcca ccacattccg ataataagga ttatgcccga
      721 atccatatac attagggggt tgatagttag cctcctccac aatacggtca tcatcatcat
      781 attcttcaaa ttcccacttt tcaatcttat taactgccac tcctcctact ttaaccgtac
      841 ctttttcacc tataatagtg atagaccctt caaagttttt gggataagtg agcattgtca
      901 cattcaaagt ggcaataata ccggaacgga aatgcaaaat ggcacaacca gtgtcctctg
      961 cttcaatatg acgagccatc gtggcagtgt aagccatcac actgccgaca tttcctaaaa
     1021 gccagtaaag agcatctaca taatgacttg cttgatttaa aaaagcacca ccatcaaatt
     1081 cccaggttcc acgccattta gcggcatcat aataagattg aggtctttgc cagaatacat
     1141 tggattgagc catataaata cggccaaaac gacccttatc cactgctcgt ttaagcagtt
     1201 gcatggtagt attgagacgg ttttgtttca ctacaaaaag ctgaacattg ttttcatcac
     1261 aagcgtgaat aagtttatcc gccccttcca cattgattgc cataggcttt tcagtaatta
     1321 cattaatttt gtgattggca acatctatac caatggaagg atgaagacca cttggggtgc
     1381 aaatactaac ggcatcaagt tgttctttat ccagcatctt atgataatct gtataaactg
     1441 aaggaatatt ataggtttta gcagcttctt ctgctctttc acgaataata tccgctaccg
     1501 ctacatattc tgcctccgga atttgtgata tagcttcaaa atgattttta gagatgcgtc
     1561 cacatcctat tagacccaac cgtaattttt ccatctattt ctccttattt tcaatataat
     1621 ctaaatacca ggtgcacatt tcggaaatac tttcctcaat gctctttctt tggacaaaac
     1681 ccgcttgcct gattttctga gaacaaaaat tagtcgccat tgataattta cgcattctgt
     1741 cgctattgat tggaatgtca atagaaaaaa gttttgcggg tatatcaaac aatttaccaa
     1801 taaaaattgc taaccaatca gggatagtta tctttggcat atgaaaacca ggattagcag
     1861 ctatgatatg cataagctca ttcaaagtta tataaggttc atccacacag ttgaaatgct
     1921 gataaccagg cttaagtaag ctcatagaaa aaagtatcat atcaattacg gtatccagag
     1981 aaacaatgct tttaacataa tcgcctttgc ccacagtgaa cagaggtgtg cgatgaagag
     2041 tatctattaa tttataaata ttagcgaaat tatgaggtcc ataaatagca gtgggacgaa
     2101 gaacaataag ctcattttca ggattctttt tttgccactc aatacataac aattctccgc
     2161 ccagtttgga ttcaccatat ggatgatctg gattataagg agtgtcttca tcagctctgc
     2221 cttgaggatg tccaaaaacg gaaactgtac tgatatagat aattttatga atgtcagctt
     2281 gttcagcgaa ttctaataat acctgagttc catactgatt atgactgaaa tattcctctc
     2341 gggatattcc aaaatcgtgt tttgatgctg cacaatgaat tatcagctca atttgatggt
     2401 tatatttttg ataaaagata tcttggactt tttctaaatc tttactggaa cgaatatcac
     2461 ctataaccgt ttctggatga ggttttgcat cacaatgaga acttatatca attcctatca
     2521 ccttatgatt taacttttcc aattcccaca ttaagcgaga acctacaaaa ccattacttc
     2581 cagtaaccag tatattcatt ggatattatc tcctctttag ttttaaccca ctttaaatct
     2641 tataatttgg aaatcttttt gaataaatta atattcaata taatctcctt ctacccttaa
     2701 aaagagattg aatataaact gtcaatttaa aagaatccca gtaataatgg taataaaaat
     2761 cttaggattt ctgcagtctt cattaatcat taaaaaggtt aggtgatttt ttagaaggag
     2821 tgctattata taaattttct aaatctttaa ataagcgatt catactatat ttggaaagca
     2881 cctcttcagc cgcttgcttt cttaaatagt caagattctg aaaattatcc acgcaataga
     2941 ttatctgttc agcaatagct tgtgggttag catcacataa aaaaccattt tctccatttt
     3001 ttacaaagtc cgggactcca ccaactggag tagcgattac aatcttttga gaagccatag
     3061 cttccagaag ggagacaggt gttccttcgt ttatagaggt taggcataca atatccactg
     3121 agccataaat taactttaaa tcttctatga aacctgggaa agtgaaataa tcttgtattc
     3181 ctaattgatt taccagcttc ttacaatcat cggttaattc tccatctcct attatgagga
     3241 aatggacatt atttctttgt ttcagcactt cgtttgcgct ataaataaaa agagctggat
     3301 tttttatggc tgttactcta cctatcaggg caatagtaat tttgtcctta gcgatgttta
     3361 acttttttct taatttgcat tcattatcat agggtaataa tctggaaaaa tcaaaaccaa
     3421 gagggataat agttatttta tcagggcgaa gagtaaatcc agggccctgg agtagtggta
     3481 tacaccctgn nnnnnnnnnc aattttaaat ctaatcagat cttctttttg ttgttctgaa
     3541 attgcgatga ttttagtaga aaatcgggca agaaatcttt ctatttgaac gaaaaatttg
     3601 gttttaagag gtgagaaata tccttcaaaa gtatgaccat gaaaagtatg ataaatctga
     3661 ggaactccag ttaaaattgc agctaaccgt cctaaagtcc cagctttaga ggtgtgagta
     3721 tgcactatat caggtttctc tttttttaga atttttatta attgtatttc tgccaaaaaa
     3781 tc
//
LOCUS       DAORHB010000137         3751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_26920_length_3751_cov_4.072781, whole genome shotgun sequence.
ACCESSION   DAORHB010000137 DAORHB010000000
VERSION     DAORHB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26920_length_3751_cov_4.072781"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /locus_tag="PKM80_04685"
     CDS             complement(<1..1776)
                     /locus_tag="PKM80_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV93240.1"
                     /translation="MFIDHRYEVLEHLGTGAWANVYKVRDIRSGKLYSLKLFQYVSSD
                     DLYSRFSAEEMHHITRLEHPNLSHTVDFGHVADHIYYISEYFEGKPLTGVKFTKAKLG
                     QIYDIAIQICYALHALHTQNILHKDLKLENVLYRTEGNTIILRLIDYGFSKVDTTKDS
                     QAVSGTLPYLAPEIYLGKPASKASDFYALGVILYKLTTGSFPFSVDQINALITGGNQY
                     FIPSFPSELNKDIPLSLEKFILRLLDQNPDNRFASCEEIVHYINRTQSFQYPFSVSWS
                     MINTLHFNSYLVRDKYSHQILDYIPAVEKGNGKIISVTGGDGLGKDSIISLFRYHLLS
                     GKYFIFDYTCSKSDHEAFFALIKEFVQSLSPEELQEYSGLKTISEKFRCYLFGSEQEA
                     KTVSQTSSELKLDFESVKSLLIDLSINKPIIFIIRNFQYVHHNTIDFINFMSSYIVKH
                     RIMIVITCNDYNKINQIDHTVLMNIPTLNLDESRNYINRLLSGFSSSDNRDSVPEELI
                     NEIYRRSAGNPYFIVEILIDLMQRRKLYYDKMLHYPADLSDYQLSSRLLQSIHSRLSH
                     LKDVNYLHLQKLSVVQTPLTRELIIY"
     gene            complement(1817..2704)
                     /locus_tag="PKM80_04690"
     CDS             complement(1817..2704)
                     /locus_tag="PKM80_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV93241.1"
                     /translation="MPNHKLKNRIEYVSFRIALAGLKLLPPEAAKAVLKGLFWFGGWV
                     IGIRKNVALQQLSKIFPDKNSKEKAAIIKKLYRNMALSIYECYLMDDETLYKNIRIEG
                     REFIEEALSLKRGAIFATAHYGNWEAARILPKAGIPISGIAKPQRNVLFDKYTNTIRE
                     RCGMRIINMKRGLRDIVHEFRENRIVAILIDQNAGGSGLLMNFLGFPASHWKGVAKLS
                     LRYRVPIVPCFARRSEDDRIVFEFFPVILHNDLDDKEENYKIVLEEVNKILEHQIREH
                     PEQWFWVHLRWKHSYDMLA"
     gene            complement(2784..3095)
                     /gene="trxA"
                     /locus_tag="PKM80_04695"
     CDS             complement(2784..3095)
                     /gene="trxA"
                     /locus_tag="PKM80_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNV93242.1"
                     /translation="MIKDLNEVDFDKAIAGDKVIIDFWAPWCGPCKIFSPTLDEIDKE
                     VADLTIYKVNVDNYPALAGRQNVHSIPTLLFYRRGKLVDSSIGVVSKSVILQKLERMN
                     D"
     gene            3566..>3751
                     /locus_tag="PKM80_04700"
     CDS             3566..>3751
                     /locus_tag="PKM80_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNV93243.1"
                     /translation="MLRGNEIKPAEGKLGVLLVGLGAVSTTFVAGVQAIKKGISEPYG
                     SVSQMSTIRLGKRTENRT"
BASE COUNT         1211 a          705 c          595 g         1240 t
ORIGIN      
        1 atagattatc aattctcggg ttaaaggagt ttgcactacg cttagtttct gcaagtgtaa
       61 ataatttaca tcttttaggt gactcaagcg cgagtgaatt gattgcaata aacgagatga
      121 taattgataa tcagaaagat cggcaggata gtgtaacatt ttatcgtaat atagctttct
      181 tctttgcata agatcaatca atatctccac aatgaagtaa ggattccctg cagagcgtct
      241 atatatttca ttaattaatt cttcaggaac actatctcta ttgtcagaag aggaaaaacc
      301 ggatagcaaa cgattgatat agttccggct ttcatccaaa tttagagtag ggatgttcat
      361 tagcactgta tggtctatct gatttatttt attataatcg ttacaggtaa taacaatcat
      421 tatacggtgt ttaacaatat aagaggacat aaaattaata aaatcaatcg tattatgatg
      481 cacatactgg aagttgcgaa tgataaaaat aataggttta ttaattgaaa ggtctataag
      541 caaacttttt acagattcaa aatcaagctt caattcacta cttgtctggc ttacagtttt
      601 ggcttcctgt tctgaaccaa ataaatagca acggaatttt tcagaaatcg ttttcagccc
      661 actgtattcc tgaagttcct cgggacttag agattgaaca aattccttta ttaaagcaaa
      721 aaatgcctca tgatcgcttt tggaacaggt gtaatcaaaa ataaagtact taccgcttaa
      781 taaatgatag cggaataagg agataatact atctttgcct aatccatctc ctccagttac
      841 tgatataatt ttcccatttc ctttttctac tgcaggaatg taatctaaaa tctgatgtga
      901 atatttatcc cgaacaagat aactattaaa atgtaaggtg tttatcatag accaggacac
      961 cgagaaagga tattggaagg attgagtccg atttatgtaa tgcacaatct cttcgcaact
     1021 agcaaagcgg ttatcaggat tttgatctag taaacgtaat ataaactttt ccagagaaag
     1081 aggaatatct ttattcagtt cactgggaaa actgggaata aaatattggt taccaccagt
     1141 aataagtgcg tttatttgat ccacactaaa aggaaaacta ccggtagtaa gtttatataa
     1201 aattactccc aaggcataaa aatcgctagc tttgcttgcg ggttttccta aataaatttc
     1261 aggagcaaga taaggtaatg ttcccgaaac tgcctgtgaa tctttagtag tatcaacttt
     1321 ggaaaagccg taatcaatca gcctaagaat aattgtattc ccttccgtcc tatacaatac
     1381 attctccagt ttcaagtcct tatgcagaat attttgagtg tgtaaagcat gcaaagcata
     1441 acatatctga atagcaatat cataaatttg gcctaattta gctttagtga atttgacacc
     1501 agtaagcggt ttaccctcaa aatattcact gatataataa atatgatctg caacatgccc
     1561 aaaatcgaca gtatgactaa gattgggatg ttcaagacga gttatatgat gcatttcttc
     1621 agcgctgaaa cgcgagtata aatcatctga agagacatat tggaaaagct ttaaagaata
     1681 aagttttccg ctgcgaatat cgcgaacttt atatacatta gcccatgcac ctgtccccag
     1741 atgttctaaa acttcatatc gatgatctat aaacatataa ctgctccata gctatttcag
     1801 gatgcgaaaa caacccttag gcaagcatat cgtaggaatg tttccatctt aaatgtaccc
     1861 aaaaccattg ttctggatgt tctctgattt gatgttcaag aattttattt acttcttcta
     1921 gaacaatctt atagttttct tccttatcat ctaaatcatt atgcaagata acaggaaaga
     1981 attcaaaaac tattctatca tcttccgacc tgcgagcaaa acagggaacg ataggaactc
     2041 tatagcgcaa agagagttta gcaactcctt tccagtgaga agcgggaaaa cctaaaaaat
     2101 tcattaacaa acctgatcca cctgcatttt gatcgataag aattgctaca atacgattct
     2161 ctctaaattc gtggactata tccctaagtc cacgtttcat attgattatc cgcatcccac
     2221 atcgttcgcg tattgtatta gtatatttat caaatagaac atttctttgg ggtttagcaa
     2281 taccacttat tggaattcct gctttaggta aaatccttgc cgcttcccaa tttccataat
     2341 gagctgtggc aaatattgct cctcttttca gggataatgc ttcttctata aactctctac
     2401 cttctattcg gatattttta tatagtgttt catcatccat taggtagcac tcataaatgg
     2461 ataatgccat attacgatat agttttttga taatagcagc tttctctttg ctgtttttat
     2521 ccggaaaaat cttactaagt tgttgtaacg caacattttt gcgaatacct ataacccaac
     2581 ccccaaacca aaacaagcct tttaatacag cttttgctgc ttcaggaggt aaaagtttca
     2641 atccagcaag tgctattctg aaacttacat attctattct attttttagc ttatgattgg
     2701 gcacttaata ttcctcttga gtaagaattt tctcaagaca ccattatttt caagtgccct
     2761 gaactgacaa gtaatatctt gatttaatcg ttcattcgtt ccagtttttg caaaatcaca
     2821 ctttttgata ctactcctat tgagctatct actaatttac ctctacgata aaacaacaga
     2881 gtaggtatac tgtgaacatt ttgtctacca gctaaggcag gatagttgtc cacatttact
     2941 ttatagattg taaggtcagc aacctcttta tcaatctcat ctaaagtggg cgagaaaatc
     3001 ttacagggac cacaccaggg tgcccaaaag tcaattataa ctttgtctcc ggcaattgcc
     3061 ttgtcaaaat ctacttcgtt taagtcctta atcatctttg gtgtcctcac tagtttttat
     3121 ctgtgtttat attaattaaa gtccttcttc tctatctaac cgatgaatac taatacattt
     3181 tctaagctgt ccttaaaatt agtctcatgc tccaaaactc ttccccagcc ttttatactc
     3241 attttcaaca aaatacttat atataatata agctgaattc ctcactttgg caatgaatat
     3301 cggcaatgtc tgttgtaaac ctctttttta caattccgaa taatttctta tcttcccata
     3361 atagactatt atcgtatttt ccctcgtaac tgttatatta ctcaagttat tttcatttta
     3421 ttgtatagag ggttacatac tgttttagct gaaaaagatc tcccaattat atatgcaaat
     3481 tttcccattg cggattgtac ttgacatcag tacaatttaa ttattagtga ctcaaaatat
     3541 gcaaatatta gtaaggagaa aaacaatgct tagaggtaat gaaataaaac ccgccgaggg
     3601 caaacttggt gtccttttag ttggcttagg tgcagttagc acaacttttg tggctggtgt
     3661 tcaggcaatt aaaaaaggca tttccgaacc ctacggttcc gtttctcaaa tgtcaaccat
     3721 ccgtctaggg aaaaggacag aaaatagaac c
//
LOCUS       DAORHB010000138         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_27351_length_3715_cov_3.876230, whole genome shotgun sequence.
ACCESSION   DAORHB010000138 DAORHB010000000
VERSION     DAORHB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27351_length_3715_cov_3.876230"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1240)
                     /locus_tag="PKM80_04705"
     CDS             complement(32..1240)
                     /locus_tag="PKM80_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNV93244.1"
                     /translation="MKILLITYYFPPCGGAAVQRWLRFINALDKKGVEIFVVTTLDGD
                     YPYRDESLLSKIPATVKVLRSKPISFGKLWNILGQKELPYGSLQNKTTDKPLQKFLYW
                     LRLNVIVPDLRIGWNPAALKSAVQVLRTNKIDYVITTGPPHSTHLIGLKLKKRYNIQW
                     RTDFRDPMSEIYYLKLNPPMRLTMALHKYLERKIIRTAELNYIVSKAIANSLPEGNKE
                     VLYNGFDPDDFKDLHYERTNRFRIKYVGQLTAGQDPTPLINALTEIKLPNIDFSLIGT
                     RNFPETDFPVNRIPFLPHQLALQELINAELLILIINTYEGNEGMLTTKLFEYIASRSP
                     ILCLADSDGEAYELICKTESGFVSKNTEEIIEIINKLYERFSKGDNLRTKGDISFLDV
                     NNQIQALLKV"
     gene            complement(1240..2328)
                     /locus_tag="PKM80_04710"
     CDS             complement(1240..2328)
                     /locus_tag="PKM80_04710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV93245.1"
                     /translation="MKRIAILGPAPPYRGGISLFAFSLAEAYRDLGYQVQFINFKEQY
                     PQLLFPGKGQYDYTITDNEFVNKRILVPWQPKTWGQTIYEIKQYKPDAIIVSWFLPYF
                     APAYGYIIKRIPNTPIIILAHNILAHEKWILEKQLLKYVFKPATKIVTLSKATLEELH
                     NELPLYISKKGVLGFHPCYEHYISEVVPFPQRNTLLFFGLIKPYKGLDVLLRAMPKVI
                     NAVPDTKLIIAGEVYGKCDVYTELINSLGIGSKVECYFRFISDPEIAHFFSQSCLCIL
                     PYKSASQSGVIATSYSFGVPVLASNVGGLGEYVAEGETGYLVPPDNPSALAEKIILHL
                     TQKPDMRKAIDNFTKRYSWKALAELTLQ"
     gene            complement(2325..3302)
                     /locus_tag="PKM80_04715"
     CDS             complement(2325..3302)
                     /locus_tag="PKM80_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNV93246.1"
                     /translation="MKKNILIILTGGTISMKASDPAGVIPSNEFADFLRQFPQLNSVA
                     NVEVMDYLNIPSPYMTPEMMFELAKLVDLKIIDYDGIVITHGTDTLEETAYLCDLVLT
                     TRKPVIFTAAMRSGNEIGLDGPRNIIGSVRVACHPDSIDKGVLVVMNDEIHTARDVVK
                     SDTGKIDSFISPGYGPVGIVDPDKIVYHRTSLYKDNVWTDQLDTKVELIKAAAGMDGK
                     FIRCCMENDIKAIVIEAFGRGNLPKSLVPDIQAAIAKSILVVITSRTYTGRVLAEYGY
                     EGGGKHLETLGCILASDMKGVKVRLKLMALFGKYHDVDIVKRFFKQSVL"
     gene            complement(3321..>3715)
                     /locus_tag="PKM80_04720"
     CDS             complement(3321..>3715)
                     /locus_tag="PKM80_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNV93247.1"
                     /translation="PITYVPFRNAIFLCAAVAWAEVIKATKIYIGAVEEDSSGYPDCR
                     EVFFKSFEKVIATGTKSELPINIITPVLHKSKSEIVQMGIKLKAPFHLSWSCYSDDEI
                     ACGVCESCLLRKRAFREAGIIDPIPYRN"
BASE COUNT         1177 a          718 c          613 g         1207 t
ORIGIN      
        1 agttatcgcg acctcctgcc ccaaaaaacc tctaaacctt caacaacgct tggatttggt
       61 tattcacatc caaaaatgaa atatcacctt tggtacggag gttatctcct ttagagaatc
      121 tttcatatag tttatttata atttcgatga tttcttctgt atttttacta acaaatccgc
      181 tttccgtttt gcagattaat tcgtatgctt ctccatctga atctgccaaa cataaaatag
      241 ggctacgaga agctatatac tcgaagagtt ttgttgttag catcccttca ttgccttcgt
      301 atgtatttat aatgagaatc aatagctcgg catttattaa ttcttgcaag gccagttgat
      361 gaggcaaaaa aggtattcta ttaaccggga aatcagtttc cggaaaattt cttgttccaa
      421 tgagtgaaaa atctatgttg ggcaatttta tttctgttaa tgcatttatt aaaggagtgg
      481 gatcctgacc tgcagtaagc tgacctacat atttaatacg gaatctattc gttctttcat
      541 aatgcaaatc cttaaaatca tcgggatcaa agccattgta aagaacttct ttgttacctt
      601 caggaagaga attcgcgatg gctttactta caatataatt cagctctgca gtccgaatta
      661 ttttcctttc cagatattta tgcagagcca tagttaaacg cataggggga ttcagtttca
      721 aataatatat ttcagacata ggatcgcgaa aatcagttcg ccactgaata ttgtagcgtt
      781 tttttagttt taatccaatc agatgagtgg aatggggagg accggtagtt atcacataat
      841 caattttatt cgttcttaaa acttgaactg cacttttcaa tgctgccgga ttccagccaa
      901 tacgaagatc tggaactatg acatttaagc gtaaccaata taaaaatttt tgtagtggct
      961 tatcggttgt tttattctgt agtgatccat agggtagttc tttttgtccc aatatgttcc
     1021 ataattttcc gaaacttatt ggtttacttc gaagtacttt tacggtagca ggaattttag
     1081 aaagcaagct ttcatcccga tatggataat caccatctaa agtagtgact acaaatattt
     1141 ccactccttt tttatccaga gcattgataa atcttaacca tctttgaact gcagctcctc
     1201 cacagggagg gaaataataa gttatcagta aaatcttcat tattgcaatg ttagttctgc
     1261 tagtgctttc caggaataac gttttgtgaa gttatcaatt gctttccgca tatccggttt
     1321 ctgagtaaga tgcaaaatta ttttttcagc tagagcagaa ggattatcag gaggaaccaa
     1381 ataccctgtt tcaccttcgg ctacatattc tcccaaacca cctacattac tggctaaaac
     1441 agggactcca aaactataag aggtggcaat aaccccactt tgagaggcac ttttgtaagg
     1501 caggatacat aaacaagatt gagaaaagaa gtgcgcaatt tcaggatcgc tgataaaacg
     1561 aaaatagcat tctactttgc tcccaattcc taaagagttt attagttctg tatagacatc
     1621 acatttacca tatacttctc cagcgataat taacttggta tcaggtacag catttatcac
     1681 ttttggcatt gctctaagca aaacatccag acccttatag ggtttaatta aaccgaaaaa
     1741 aagcagagta tttctttgag gaaaaggtac aacttcactg atataatgtt cataacaggg
     1801 atggaaaccc agaactcctt ttttggaaat atatagaggc agttcgttat gtaattcctc
     1861 caaagtagct ttacttaagg ttacaatttt agttgctggc ttaaagacat atttaagcag
     1921 ctgtttttct aaaatccatt tttcgtgagc cagaatgtta tgcgccagaa taatgatagg
     1981 ggtattaggt atgcgtttta taatataacc ataagcaggt gcaaaatatg gtaaaaacca
     2041 ggaaacaatt atcgcatccg gtttatattg ttttatttcg taaattgttt gtccccaggt
     2101 tttgggttgc caaggaacca gaattcgctt attgacaaat tcattatctg ttattgtata
     2161 atcatattgt cccttacctg gaaaaagcaa ttgtggatat tgctctttaa aattgatgaa
     2221 ttgaacttga tatcctaaat ccctgtaagc ttctgctaaa gagaaagcaa aaagagaaat
     2281 tcctcctcta tatgggggag caggacccag aatagcaatc ctcttcataa tacactctgt
     2341 ttaaaaaacc tcttaacaat atcaacatcg tgatatttcc caaataaagc cataagtttt
     2401 aagcggactt ttacaccttt catatcactg gcaagaatac agccaagagt ttctaaatgt
     2461 ttccctccac cctcataacc atattcagct aaaactcttc ctgtataggt tctggaggtt
     2521 attacaacca aaatactttt tgcaatagct gcttgaatat cgggtactag acttttaggt
     2581 aaatttccac gtccaaatgc ctctattaca attgctttga tatcattttc catacagcaa
     2641 cgaatgaatt ttccatccat accagcagca gctttaataa gttccacttt ggtatctaat
     2701 tgatcagtcc aaacattatc tttgtaaagt gaagttctat gataaacaat tttatcaggg
     2761 tctacaattc caacaggtcc ataaccagga gaaataaagg aatcaatttt tccagtatcg
     2821 gattttacca catcacgggc agtgtgaatt tcatcattca tcactaccag aactccttta
     2881 tctattgaat cgggatgaca agcaacacga acacttccaa ttatgttgcg aggtccatct
     2941 aatcctattt catttccaga acgcattgca gctgtaaata taacaggttt gcgtgtagtc
     3001 aaaactaaat cacataaata agcagtttct tctaaagtat ctgttccatg agtaatcact
     3061 attccatcat aatcaattat tttcaggtct accaatttag ccagttcaaa catcatttcc
     3121 ggagtcatat agggactagg aatatttaaa taatccatca cttcaacatt agctacactg
     3181 tttaattgag gaaactgtct taaaaaatcg gcaaattcat tactgggaat aacaccagca
     3241 ggatcagacg ccttcataga aattgttcca ccggttagga taataaggat gttctttttc
     3301 atattttatc ttttttccat ttaattacga taagggatag gatcaataat ccctgcttcc
     3361 ctaaatgctc ttttgcgaag cagacaactt tcacacacac cacaggctat ctcatcatca
     3421 ctataacagc tccaggataa atgaaaagga gcttttagct ttatgcccat ttgcactatt
     3481 tctgatttgc ttttatgtaa aaccggagtt ataatattaa ttggtaattc acttttggtt
     3541 cctgtggcaa ttactttttc gaaggatttg aaaaaaactt cacgacaatc aggataaccg
     3601 gaactgtctt cttccacagc gccaatataa atttttgtag cttttatcac ttcagcccaa
     3661 gcaactgctg cacatagaaa aatagcatta cgaaaaggga cataagtaat tggaa
//
LOCUS       DAORHB010000139         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_27402_length_3710_cov_4.038304, whole genome shotgun sequence.
ACCESSION   DAORHB010000139 DAORHB010000000
VERSION     DAORHB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27402_length_3710_cov_4.038304"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PKM80_04725"
     CDS             <1..1287
                     /locus_tag="PKM80_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93248.1"
                     /translation="YFFFGVIGKQMQIDDLDKMGFALVAFIIVFIELKKVLDRRIWLL
                     GIALVLVGLSSHIYLMVRAAERPFINEGNPSNWKMFQEYVLRKQYGNTSFVQRRGNFF
                     TDQLNYHFLRYFSMQWLNEPFFTKFIGVTSNMLKPVANIFIAFLGVAGAMLQFRKNKH
                     SFWYFLSVILLTTIVMVFVMNLSNAEVRDRDYFFVVAYNMWAIWLGIGALTLVTLWKS
                     KPVRIAVLILMCLIPLSNFITQYRVHDRSQEFIALDYGLNFLNSLEENAIIFTNGDND
                     TFPLWYAQAVKDPYSKENIYSARDVYPNAESQEAVAKAMEYKNKCLKGIRKDVSVANL
                     SLLNTAWYIRQLRDKEGILFNIPDEQLDDLSIRRVEEPLVIPGPPENPAMGFTMNIPP
                     TAEWRKDEPFYRISDLAVMQIVKDNYGKRPIYFAVT"
     gene            1366..1944
                     /locus_tag="PKM80_04730"
     CDS             1366..1944
                     /locus_tag="PKM80_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93249.1"
                     /translation="MIDPQRLLHNIDVVYNYRSIDNKKVYKDDNMNRLIMNYGSGFVR
                     AANYFVDLGDYPKALEYINKARRFVHEEIKLTEFYTNYYSKTGQWNKLDEFINKTIAP
                     HPEGWKIYLSYILAYTVDKYPDKVLPYLKKGFLLFPDQEYFAHLALGYAQEYRQYDTI
                     KQLLTEVQPKLTYDVSSFISDLTSPPPMDTIQ"
     gene            2161..2958
                     /locus_tag="PKM80_04735"
     CDS             2161..2958
                     /locus_tag="PKM80_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV93250.1"
                     /translation="MRIKNPVHTLRFIVQLLFLLISMLFLAGIIVCGLPYTIHNLCPY
                     SAVCFGFSKNFLCSLNSSAFGLTIAFSFLILVLAIFFGRLFCSFICPLGTIQEYIFAL
                     FNKKSKKKKQVPHYLERRFSLVKYGVLAVTAILAIAGISYIYIRFCPIYAISLLPYIA
                     VLGLILALLIIIVGIFIERFWCRYLCPYAALLNLFQYLGKVVGFPRFKIHRNLERCND
                     CGFCSLNCPMNIDLTEEEYVNNLDCILCFRCSKKCPKPDTLVWKKDK"
     gene            2959..3510
                     /locus_tag="PKM80_04740"
     CDS             2959..3510
                     /locus_tag="PKM80_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV93251.1"
                     /translation="MKTVLQVFVLLVLSFILFFLFYNALTPHKYVDDQNTFCDKIVKD
                     DSAPKQAEADNIKIQDSELIKNPAIISDKIAIDKSATIEPKNDKPKNETSITDALPEN
                     AISNSEESSQELTNANRIVYQVDKDICIGCKLCLRICPVGAISIKKGKAVIDQDKCTG
                     CGTCAIGDGKRFKGCPVAAISAF"
BASE COUNT         1149 a          619 c          670 g         1272 t
ORIGIN      
        1 tatttctttt tcggcgttat cggaaaacag atgcaaattg atgatttaga caaaatgggt
       61 ttcgctcttg tagccttcat aattgtcttt atcgagttaa aaaaggtttt agaccgcaga
      121 atttggttat tgggaattgc cttagttctg gttggacttt catcgcatat atatttaatg
      181 gttcgagctg cagagagacc ttttattaac gaaggcaacc caagtaactg gaaaatgttc
      241 caagaatatg ttctaagaaa gcaatatggc aatacaagtt ttgttcaacg tagaggtaat
      301 ttctttactg accagcttaa ttatcatttt ttaagatatt ttagtatgca atggttgaat
      361 gaaccatttt ttactaaatt cataggggtc acttccaata tgctgaaacc tgttgccaat
      421 atatttattg ctttcttagg cgtagcagga gcaatgttac aatttcgcaa aaataaacac
      481 agcttttggt atttcttatc cgtgattctg cttactacta ttgttatggt ttttgtaatg
      541 aatctttcca atgccgaagt gcgtgaccgc gattactttt ttgttgttgc ctataatatg
      601 tgggctatct ggttgggaat tggagcctta accttagtaa ccctatggaa aagtaaacct
      661 gttcgcattg ctgtgttaat tcttatgtgc ttaataccac taagtaattt tattacacaa
      721 tacagagttc atgatagatc tcaggaattt atcgctttag attacggact aaatttcctt
      781 aattcgctgg aagaaaatgc catcatattt actaatggtg ataatgatac ttttcctctc
      841 tggtatgcac aagcagtaaa agatccctat tctaaagaga atatttactc tgccagagat
      901 gtttatccaa atgcggaaag ccaagaagca gttgctaaag cgatggaata taaaaacaaa
      961 tgcctgaaag gaatccgtaa agatgtttcc gttgccaatc tctcactact aaatactgcc
     1021 tggtatatta gacagctaag agacaaagaa gggattttat ttaatattcc ggacgagcaa
     1081 ttagacgacc tcagcattcg tagagtggaa gaacctcttg ttattccagg acctccagaa
     1141 aatcctgcta tgggatttac aatgaatatt ccgccaacag ctgaatggag aaaggatgaa
     1201 cctttctatc gcatctccga tttagcggtt atgcaaattg tgaaagataa ttatggtaaa
     1261 cgtccaattt attttgcagt tacctgagag agttttgtaa actttgagga ttatgttcgt
     1321 aatgaaggta tggtctcccg agttgtttcc aacccaggcg attcaatgat agatccacaa
     1381 cgacttttac ataatattga tgtggtttac aattaccgtt ctatagacaa taagaaagtg
     1441 tataaggacg ataatatgaa ccgtttaata atgaattatg gttctggctt tgtgcgtgct
     1501 gccaattatt ttgttgatct cggagattat cccaaggcat tagaatatat taacaaagcc
     1561 agaagattcg tccacgagga aattaagctg actgaatttt acacaaatta ctacagtaag
     1621 accggccaat ggaacaaact ggacgaattt atcaacaaga ctattgcccc ccatcctgaa
     1681 gggtggaaaa tatatctgag ctatattctt gcttatacgg tagataagta tcccgataaa
     1741 gtacttcctt acttaaaaaa gggttttctg cttttccctg accaagagta tttcgcacat
     1801 ctggcattgg gttatgctca ggaatataga caatacgaca caattaaaca gctgctaacc
     1861 gaagttcaac ctaaactaac ttatgatgtt agctctttta tatcagattt aacttctccc
     1921 cctcctatgg atactatcca ataaaatagg aaaatgttac agaattacta tatccttgca
     1981 gctagcctgt aaccttccca tatcattccc ttatcgttcc catatcgtga tatgggaatg
     2041 ttatgggaag ttattgagct atatatagta aagaactagg tacaaattaa tggatagttg
     2101 gaataattct ttttagtgta gaggatatat tttcacgatg attataaagg aactactgtt
     2161 atgcgcataa aaaatccggt tcatactctc agatttattg ttcagctcct ttttttgcta
     2221 atatctatgc tttttttagc tggcataatt gtttgtggat taccttatac tattcataat
     2281 ctttgccctt actcagctgt gtgttttggc tttagtaaaa attttctatg tagtttaaat
     2341 agttctgcat ttggtttaac tattgccttc agttttctaa tattggtttt agctatattt
     2401 ttcggacgcc ttttttgctc ttttatttgc ccgttgggaa cgattcaaga atatattttt
     2461 gctctcttca acaaaaaaag taaaaagaaa aaacaggttc cccattattt agaaaggcgt
     2521 ttcagccttg taaaatatgg tgtccttgct gtaactgcaa ttttagccat tgccggcatt
     2581 agctatatct acattcgctt ctgccctatt tatgcaatct ctttgctccc ttatattgct
     2641 gttttgggtt taattttagc tttgctgata attatcgtag gaatatttat tgagcgtttt
     2701 tggtgccgtt atctttgccc ttatgctgct ctattgaatt tatttcaata cttgggaaag
     2761 gtagtgggtt ttccgcgttt taaaatccat cgtaatttgg aacgctgtaa tgattgcgga
     2821 ttttgttcat taaactgccc gatgaatatt gatcttactg aagaagaata tgttaataat
     2881 ctggattgta ttctctgttt tcgctgttca aaaaaatgcc ccaaacctga tactctcgta
     2941 tggaaaaagg ataaataaat gaaaactgtt ctacaggtat ttgtcttatt ggttttaagt
     3001 tttatactct tctttttgtt ctacaatgct ttaacaccac ataaatatgt tgatgaccaa
     3061 aacacttttt gcgataaaat tgtaaaagat gactctgcac ccaaacaagc cgaagcagat
     3121 aacataaaaa tacaagattc tgaattgata aaaaatcctg ctataatatc tgataaaata
     3181 gcaatagata aatcagcaac aattgaacca aaaaatgata aacccaaaaa tgaaacatct
     3241 attacagatg cattacctga aaatgcaatt tccaattccg aagaaagttc ccaagaattg
     3301 actaatgcca atagaattgt ttatcaggtc gataaagata tttgtattgg atgtaaatta
     3361 tgccttcgaa tatgccctgt tggagcaatt tccataaaaa agggtaaagc cgtgatagat
     3421 caagataaat gtacaggttg tggaacttgc gcgattggag atggtaagag atttaaaggc
     3481 tgtccagttg ctgcaatatc agcattctga gtatattagc taactaatac ataataaggc
     3541 aattagcata gaactaattg atattgtagg tttgcaatat tcctttttta ttgttttgcg
     3601 cggttctttt tttatctata atgcgattgt attatcagtt tatttggtgt attgcaagaa
     3661 atgtagttgc taaaaagtgg ggtatccata agaatggcat atagttaaat
//
LOCUS       DAORHB010000140         3649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_28058_length_3649_cov_3.473567, whole genome shotgun sequence.
ACCESSION   DAORHB010000140 DAORHB010000000
VERSION     DAORHB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28058_length_3649_cov_3.473567"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PKM80_04745"
     CDS             complement(<1..1160)
                     /locus_tag="PKM80_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNV93252.1"
                     /translation="MNLLQIINLVGGLALFIFGINKMSDSLQAVAGSEMRRILKVLIN
                     TPLKGVLVGIGVTALIQSSSATTVMMIGLVNTGIMTLQQAVGVVMGANIGTTITAQLI
                     AFDLGQYAFVFIILGVTLIFIRKSRAMEHWSQIIFGFGLLFLGLNVMAESVAPLQNSV
                     VARNLMIKLASNPIMSILVGTGFTMLIQSSAASIGIVMVLASTGLIPFEGALYLVFGD
                     NIGTTITAWLAAIGSSNTAKRVAMVHTLFNVFGTIIFGLLTYLGLYPLFVNWITPGNV
                     YAGQNIARHIANGHTMFNIVNTIVFIPFAGQLAKIATKIIPDDKVETISLGEPKHLNY
                     TIINNSYLAINQSIKEMREMLRLVRMELLVTYQAFKEKDYKKHMRVSRIEKA"
     gene            1343..1612
                     /locus_tag="PKM80_04750"
     CDS             1343..1612
                     /locus_tag="PKM80_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNV93253.1"
                     /translation="MPTWEIYVNGRVQGVGFRYFVWKAATELGIKGYVQNQWDGKVKI
                     VAQAEEHTLHTFQIIVAQGNRYAFVKELKINKLDSAEKYNDFEIR"
     gene            1593..2801
                     /locus_tag="PKM80_04755"
     CDS             1593..2801
                     /locus_tag="PKM80_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV93254.1"
                     /translation="MISKLDKYLWLMLMQIMFILPLGLSAVDQIHTVKKGDTLYSLSK
                     RYGTTIDELKKLNSISGNNLSIGQKLIIKKDVPKPKPVTPAPAIKPIPTEIPEGSSPP
                     PISGASTTENISLPTIPITIPSEYYYTVKAGDNLYRIAVNNNITLKELLDWNNFANSS
                     VRIYPGDKLLIKNPSEVSETKIAPVEAVKDPVASINLTPTEEDTILIERVYIVQKKDT
                     LYRIATNNGMTVEELMQLNNLTSTNLYVGQKLYLSGKPRLVPSTPPEILTEEELMKRD
                     KIRTDLIMPIEGHIISEYGLRNGRPHKGIDIGAKTGTPVYAVLDGTVVYSGVQGSYGN
                     VVVIEHPDFVMTVYAHNERNLVSVNDVVKQGQQIATVGSTGNAQGSHLHFEYRLKGKA
                     INPRKVLPLK"
     gene            2814..>3649
                     /locus_tag="PKM80_04760"
     CDS             2814..>3649
                     /locus_tag="PKM80_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNV93255.1"
                     /translation="MSRKDSVFADKALQNYLNEISKFKTLSREEERELAIRAKNGDVE
                     AMNRLIQANLKFVVKIAARYQNRGLSLSELISEGNIGLIKAIEKFDPDKDVKLISYAI
                     WWIKQRITLAVSEKSSLIRVPMGKTSAAHRVKATQDRYFTETGEMASTDELSEKLNIA
                     EKTIDQLQNQLPETSSFDDIITTLDSQDFTTKDLIEDKETVDPQKLYYKERMQERIQR
                     AINKLDQREADVIRSYFGLNESHESRNFAQIAESMGLSRERVRQIQKEALKKIMREIK
                     PE"
BASE COUNT         1272 a          655 c          668 g         1054 t
ORIGIN      
        1 attgctttct caattcggga gactcgcata tgcttcttgt agtccttttc tttgaatgct
       61 tgataagtaa ccaaaagctc cattctcact aaacgcagca tttcccgcat ttcttttatg
      121 gactgattta ttgccaagta ggaattgttt ataattgtgt aatttaaatg tttgggctcg
      181 cctaaagaga tagtctccac tttatcatca gggataattt tagttgcaat ttttgccagc
      241 tgccccgcga aaggtataaa tacaatagta ttaactatat taaacatagt atggccatta
      301 gcaatatgac gagcaatatt ttgccctgca tacacattgc ctggtgtaat ccaattcaca
      361 aataagggat ataatcccag ataagttaat aatccaaaaa tgattgttcc aaacacattg
      421 aataaggtgt gaaccattgc tactcgtttt gctgtattac tactgcctat tgctgccaac
      481 caagcagtta ttgtggttcc aatattatct ccaaaaacta aatataaagc accttcaaaa
      541 gggattaaac ccgtagaggc taaaaccatc actattccga tcgaggcagc agaactctga
      601 attaacattg taaatccagt tcctaccaat atactcataa taggatttga agctaattta
      661 atcattaaat tacgggctac aacagaattc tgtagaggag caactgattc cgccataaca
      721 ttcaaaccta aaaacaataa gccaaaacca aagataattt gactccaatg ttccatagct
      781 ctacttttgc gaatgaaaat taaagtaacc cctaagatta taaacacaaa agcatattga
      841 ccaagatcaa aagcgattaa ttgagcagtt atggtggttc caatattagc tcccataact
      901 acacccactg cttgttgtaa ggtcataatt ccagtattaa ctaaaccaat catcataaca
      961 gtggtagctg agctactttg aattaatgct gttacaccta ttcctactaa aactcctttt
     1021 aagggagtat taataagaac ttttaatatc ctacgcattt cggaaccagc aacagcctgt
     1081 aagctatcgc tcattttatt gattccgaaa ataaatagtg ctaatccacc cactaagttt
     1141 atgatttgca gtaagttcat ctagtccaga agttcctttt ttatatgtta agttcttact
     1201 ttttttcctc ccacgatatg gtcaaggtat ttttggttga caagaggtca aattatacct
     1261 tcctgtaaaa ataaagaaaa cgaatccttg acaaattcaa gcagttaatt tatgcagaac
     1321 aaataatcta aaggtgtgat atatgcctac ttgggaaatt tatgtgaatg gtagagttca
     1381 aggagttggt ttccgatatt ttgtttggaa agctgctacc gaacttggca ttaaaggtta
     1441 tgttcaaaac caatgggatg gaaaggtcaa aatagtcgcc caggcagaag aacatacttt
     1501 gcatactttt cagattatag tagcccaagg caatagatat gcttttgtaa aagagctaaa
     1561 aattaataaa ttggatagtg cagaaaaata taatgatttc gaaattagat aaatatcttt
     1621 ggctgatgct gatgcagata atgtttattc tgcccttagg gttatcagct gtagatcaaa
     1681 ttcataccgt taaaaaaggc gatacgctat actcgcttag caagcgatat ggcacgacta
     1741 tcgatgaact taagaaactg aatagcattt ctggtaataa cctttctatt ggccaaaagc
     1801 tgataattaa aaaagatgtt ccaaaaccta agcctgttac tcctgctcca gctataaaac
     1861 ctattcccac agaaattcca gaaggctctt ccccacctcc aatttctggt gcttcaacaa
     1921 ccgaaaacat ttccctgcca acaattccta taaccatccc ctctgaatat tattatactg
     1981 ttaaagcagg tgataatctg tataggatag ctgtgaacaa taatatcact ttgaaggaac
     2041 tgctagactg gaataacttt gctaatagtt cagtgcggat ttatcctggg gataaactac
     2101 taattaagaa tccaagtgaa gttagcgaga caaaaattgc accagttgaa gcagtaaaag
     2161 atcctgtagc atccattaat cttaccccta cagaggaaga tacaattctg attgaacgag
     2221 tatatatagt tcaaaaaaag gatactttat ataggattgc cacaaataac gggatgacgg
     2281 ttgaagaatt aatgcaactg aataatttaa cctcaaccaa tttatatgtg ggacagaaat
     2341 tatatctttc agggaaacct cgtcttgtac cttctacgcc tcccgaaatt cttacagaag
     2401 aagaattgat gaaaagagat aagatccgca ccgatctaat tatgccgatt gagggacata
     2461 ttatttccga atatggtttg cgaaatggac gcccccataa aggtatagac ataggtgcaa
     2521 aaacaggtac tcctgtttat gcagttttag acggaacagt tgtctattca ggagttcagg
     2581 gttcttatgg gaatgtagta gtaattgaac atcccgattt tgtaatgacg gtttatgctc
     2641 ataacgaaag aaatttagta agcgtaaatg atgtagtgaa acaggggcag caaattgcta
     2701 cggttggttc aaccggtaat gctcaaggca gtcatttaca ttttgaatac cgtttgaaag
     2761 gaaaggccat caatccccgt aaggttttgc ctttaaaata aggaataaga agagtgagcc
     2821 gtaaagatag tgtttttgca gataaagccc tacagaatta tctgaacgaa atatccaagt
     2881 ttaaaacact atcgcgcgag gaagaacgag aacttgctat aagagccaaa aatggagatg
     2941 tggaagcaat gaatcgtcta attcaggcga atctgaagtt tgtggtaaaa attgcagctc
     3001 gttatcaaaa tcggggatta tccctttcgg aacttatcag tgaaggcaat attggactga
     3061 ttaaagctat tgaaaagttc gatccagata aggatgtaaa gcttatctct tatgctatct
     3121 ggtggattaa acaaagaatt accttggctg taagtgaaaa aagttctctg ataagagttc
     3181 ctatgggaaa aacaagtgca gctcacagag taaaagctac tcaagataga tatttcactg
     3241 aaaccggaga aatggcttcc acggatgaac ttagcgaaaa acttaatatc gctgaaaaaa
     3301 ctatcgatca attgcaaaac caattgccag aaacaagttc ttttgacgat attatcacta
     3361 cactcgatag tcaggatttt actaccaaag acctcattga agataaagaa acagttgatc
     3421 cgcaaaaatt atattataag gaacgaatgc aagaaagaat tcagagagct ataaataaac
     3481 ttgatcagcg tgaagctgat gtaattcgta gttattttgg tttgaatgaa tctcacgaat
     3541 cgcgaaattt tgcccaaata gccgaatcaa tgggtttatc aagagaaaga gtaaggcaaa
     3601 ttcaaaagga agcattaaag aaaataatgc gtgagattaa acctgagga
//
LOCUS       DAORHB010000141         3633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_28248_length_3633_cov_4.632476, whole genome shotgun sequence.
ACCESSION   DAORHB010000141 DAORHB010000000
VERSION     DAORHB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28248_length_3633_cov_4.632476"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKM80_04765"
     CDS             <1..1096
                     /locus_tag="PKM80_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV93256.1"
                     /translation="MRYSNVSKNSVDINTRYLQGGLLLMDNNTGQVLAMIGGRNFTHS
                     KYNRITQAKRQPGSSIKPTYYTAALEKGYTPATVIIDSPITLTGGDGKEWTPQNFSRQ
                     YYGPTRMRTAITYSYNVWAVKAVMDIGLDVVNDAFLRFGINTKAADYSAALGAYEVTP
                     LNLISAFTTFPNGGYRTKPIFITRVEDMNGKVLERSATSKYQVTSPEVAFLMTSMLQS
                     VVTSGTGAAARNNYYWQAAGKTGTSSDHRDAWFIGFNKKYTLGIWNGFDNNAAISASA
                     SCAPIWGQIMTKAIRIDNKGRTPRNDDPRYSFVVPDRIVKRTINPKTGFLSDTGIEEY
                     FIEDNIPSARSDTLSYNFYPTRWGTKSKLD"
     gene            1100..2269
                     /locus_tag="PKM80_04770"
     CDS             1100..2269
                     /locus_tag="PKM80_04770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV93257.1"
                     /translation="MSSVIFYILSALILTGCITYFVFCIRFWNGYRTYKPTFNYKKRT
                     VSVVIAARNEAKNLQELLLCLLNQDYPKELFEVILADDDSEDDTEQVAKKYIDAGLNL
                     HYLKVLGRENVISPKKKAMEDAVYATQGEIILTTDADCLVPETWISSMVSMFTDDVSM
                     VAGYSRTYIPDWKKASLVQKYEHLDFALTYMVLAGGYTIGKKWACIGQNLAYRRTAFD
                     DVGGFTKIKHLLSGDDVNLLQLMRNKGHKIIFNFLPSSFVYTHPVTSWKQLINQRSRW
                     ASNMKYQLKFNPEFFFILLSMAFMYWGGLIMLFFNIKIALIIFLYRIIVENITISYSR
                     SRFGFTKEMLRFYPIWLVIQNFFLVLTMVLGQFNIFVWKGKRPTSKRNNNANHNI"
     gene            2250..3443
                     /locus_tag="PKM80_04775"
     CDS             2250..3443
                     /locus_tag="PKM80_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HNV93258.1"
                     /translation="MQTIIFDDASRSNFFPLTLNRSIGDLRCGITKLRQRLEHIFSDT
                     DKTCVIIDDSLVPLYKEHHPTWIINSLVNGEKLYLNSRIIVTVEAIAQIKDLPMGYCL
                     YSGNDLVAIKVNDSSVWPDLLKLKKKLGNIRLYDSLADLIQDNSRSLVRDYHLHFYDG
                     DNYFETEPGVTVLHPYNIWVAEGAEFSPGVVLDASEGPIIIDTSVRIMANAVICGPVY
                     IGKKSVIKIGAKIYGGTSIGPVCKVGGELEGTIFQAYSNKQHDGFLGHSYIGEWVNLG
                     ADTNNSDLKNTYKNVAFYSYPLKTKIDSSTQFLGCMIGDHSKTGIGCSINTGTVIGIG
                     CNLYGPNLIRDFVPDFSWGQADELDKHRFSAFCETAAIVKQRRKLIFTDTERELYHQI
                     YNMRL"
     gene            3443..>3633
                     /locus_tag="PKM80_04780"
     CDS             3443..>3633
                     /locus_tag="PKM80_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tpl protein"
                     /protein_id="HNV93259.1"
                     /translation="MQQYIEVEFRTGRIGYYRNREELNIQPENLIIVEVERGDDIAQV
                     IHLGLEEDEIAPEVLTSKNL"
BASE COUNT         1122 a          585 c          719 g         1207 t
ORIGIN      
        1 tatgcgttac agcaatgttt ctaaaaacag tgtggatatt aatactcgtt atctacaagg
       61 aggattattg ttgatggata ataatacagg gcaggtttta gctatgattg gaggtagaaa
      121 ttttacgcat agcaaatata atcgtattac tcaagctaag cgtcaaccgg gaagctctat
      181 taaaccaacc tattatactg ctgcattgga aaaaggttat acacctgcca ctgttataat
      241 agattctcca attactctta ccggaggaga tggtaaggaa tggactccac aaaattttag
      301 ccgtcaatat tatggaccaa ccagaatgcg aactgctata acttattcct ataatgtttg
      361 ggcagtgaaa gctgttatgg acattggact tgatgttgtt aatgatgctt ttctccgttt
      421 cggaataaat actaaagcag cagattattc tgcagctttg ggtgcatatg aagttactcc
      481 attaaaccta atttctgctt ttacgacttt tcccaatgga ggatatcgaa ctaaaccaat
      541 atttataacc agagtggaag atatgaatgg aaaagtatta gaacgtagtg ctacttctaa
      601 atatcaggtt accagccccg aagtagcatt tttaatgact tctatgcttc aatcagttgt
      661 cacttctgga acgggagctg ctgccagaaa taattattat tggcaggcag ctggaaaaac
      721 aggtacttcc agtgaccatc gtgacgcctg gtttattggt tttaacaaaa aatacacttt
      781 aggaatttgg aatggttttg acaataatgc agcaatttct gcaagtgcaa gttgtgctcc
      841 tatttggggt caaattatga ctaaggcaat acgaattgat aataaagggc gtacccctcg
      901 aaatgatgat ccacgctata gttttgttgt tcccgataga attgtgaaac ggacaataaa
      961 tcctaaaacc ggttttcttt cagatactgg tatcgaggag tattttatag aggacaatat
     1021 tccctctgct aggtctgata ctttaagcta taatttttat ccaacccgtt ggggaacgaa
     1081 aagtaaattg gactaataga tgagcagcgt tattttttat atccttagtg ctttaatatt
     1141 aacaggatgc ataacctact tcgtgttttg tatccgtttt tggaatggct atagaactta
     1201 taaacccacc ttcaattata aaaaaagaac tgttagtgtc gttatcgcag ctcgcaatga
     1261 agccaaaaat ttacaggagt tgttactgtg tttgcttaat caggattacc ctaaagaact
     1321 atttgaagtt attttagcag atgatgattc tgaagatgat acagagcaag tagccaaaaa
     1381 atatattgat gccggtttaa atctacatta cttgaaagtt ctgggcaggg agaatgttat
     1441 ttcacctaaa aagaaagcta tggaagatgc agtatatgca acgcaaggag aaattattct
     1501 tactacggat gctgattgcc ttgtccctga aacctggatt tcttctatgg tttctatgtt
     1561 tacggatgat gtttctatgg tggcaggata ttccagaact tatattccag attggaaaaa
     1621 ggcatcactt gtgcagaaat atgaacatct tgattttgct ttaacttata tggttttggc
     1681 tggtggttac acaattggta aaaaatgggc ttgtattggg caaaatcttg cctatagaag
     1741 aacagctttt gatgatgtag gtggctttac taaaataaaa catttgcttt ccggtgatga
     1801 tgtgaatctc ttacaattaa tgcgcaataa ggggcacaaa ataattttca attttctacc
     1861 ttccagtttt gtatatactc atcctgttac ttcctggaaa caactaataa atcaacgaag
     1921 tcgttgggct tctaatatga aatatcagct gaagttcaat ccggagttct tttttatcct
     1981 actttctatg gcttttatgt attggggtgg attgataatg ttgtttttta atatcaagat
     2041 tgctctgatt atttttcttt accggattat cgtagaaaac atcactataa gttattctcg
     2101 ttcccgtttt ggatttacca aggaaatgct tagattttat cctatctggt tagttatcca
     2161 gaattttttc ttggttttaa cgatggtctt aggacagttt aatatctttg tttggaaagg
     2221 aaaacgcccc actagcaaaa ggaataataa tgcaaaccat aatatttgac gatgcttcgc
     2281 gctctaactt ttttccatta actttaaaca gaagtattgg tgatctccgt tgcggaataa
     2341 ctaaactccg tcagcgtctg gaacatattt tttctgatac tgataaaact tgcgtgataa
     2401 tagatgattc acttgttccc ttatataaag aacatcatcc tacttggatt ataaattctc
     2461 ttgtaaatgg tgaaaagcta tatttgaatt cacgcataat tgtaacggtg gaagctattg
     2521 ctcagattaa agatcttcct atgggctatt gtttatattc aggtaatgac ctggttgcaa
     2581 taaaagttaa tgattcttca gtttggccag atttattaaa gctgaagaaa aagctcggca
     2641 atattagact gtatgactct cttgctgatt taattcagga taattccaga tcacttgtta
     2701 gggattatca tttgcacttt tatgatggag ataattattt tgaaacggag cccggagtta
     2761 ctgttcttca tccttacaat atttgggttg cagaaggtgc ggaattttct ccaggggttg
     2821 ttttagatgc ttctgaagga cccatcatca ttgataccag tgtccgcatt atggctaatg
     2881 ctgttatttg tggacctgtt tatataggca agaaatccgt aattaaaatt ggggctaaaa
     2941 tttacggagg aacttctatc ggtcccgttt gtaaagtagg tggagaattg gaaggaacta
     3001 tttttcaggc atatagcaat aaacaacatg acggttttct aggtcattcc tacattggtg
     3061 aatgggtaaa tcttggggct gatacaaata atagtgatct gaaaaatact tacaaaaatg
     3121 tggctttcta ttcctatcct cttaaaacta aaatagattc atccacacag tttttgggtt
     3181 gtatgattgg agaccacagt aaaaccggta ttggatgcag tatcaataca ggaaccgtta
     3241 ttggaatcgg atgtaatctt tatggcccta atttgattcg agattttgtt ccggattttt
     3301 cttgggggca agcagatgaa ttagataagc atcgtttttc tgctttttgt gaaacagcgg
     3361 caattgttaa acaaaggcgt aagctaattt ttacagatac agaaagagag ctttatcacc
     3421 aaatatataa tatgaggtta taatgcaaca atatatagaa gtagaattta gaaccggtcg
     3481 tataggttat tatcgaaatc gtgaagaact taatattcaa cctgaaaacc taatcatcgt
     3541 agaagttgaa cgaggtgatg atatagctca ggtaattcat ttgggcttag aagaagacga
     3601 aattgctcct gaagttctta cttccaaaaa tct
//
LOCUS       DAORHB010000142         3628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_28302_length_3628_cov_4.525889, whole genome shotgun sequence.
ACCESSION   DAORHB010000142 DAORHB010000000
VERSION     DAORHB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28302_length_3628_cov_4.525889"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PKM80_04785"
     CDS             complement(<1..1101)
                     /locus_tag="PKM80_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNV93260.1"
                     /translation="MELKKLQQMIEENAVKAVDLKYCGLDGKWYHITFPARNIAEVME
                     SGIPFDGSSIPGMTSVESGDMVLMPDASTAQLDAFNATPTLRMICSICDAETRIGVNK
                     DPRSVALRAHKYLLSTGIADMSTWIPELEFHLFDTVQYYSDAFSSGYNLTSSECKDAL
                     PEDNEDLDALALQGVKGYHMDTPFDQFYEIRQKIVELIEEQDIKVRYHHHEVGLSAQE
                     EIETELLAFPKICDDVMVMKDIIRRTALQYGLTATFMPKPVYGHAGNGMHFHIMLHKN
                     GKNLFYKKGGYADLSDEAIWFIGGVLTHGRALVAFTNPSTNSYKRLLPGFEAPVKLFY
                     GLANRSAAIRIPKYVNTPETKRFEFRTGDGTCN"
     gene            complement(1361..2005)
                     /locus_tag="PKM80_04790"
     CDS             complement(1361..2005)
                     /locus_tag="PKM80_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNV93261.1"
                     /translation="MNNSNVISLLLNVVIIALVFYSIIIHEFSHSLAAFLLGDDSAKR
                     AGRLTLNPLKHIDWFGTVILPLFLYFTCGFIYGYAKPVPFNPYNFKNFKTDSGLTGLA
                     GPLSNFLVAIVFALIYHLFGFSFIVQYICTYVIYLNLLLAFFNLIPIPPLDGSKVIGI
                     FLTDQAYYKWTMQERKGMIYLFAFIIISSLLGWNIIGKIIVPPVNFIMNLMGVH"
     gene            complement(2002..3204)
                     /gene="tyrS"
                     /locus_tag="PKM80_04795"
     CDS             complement(2002..3204)
                     /gene="tyrS"
                     /locus_tag="PKM80_04795"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNV93262.1"
                     /translation="MAFEQEMKLISRGVEEIITREELEAKFAKSVKTGRPLRVKYGID
                     PTGCDVHLGHLVPIRKMRQFQDLGHIGVIIIGDFTAQIGDPTGRDESRPPLTSEQVIK
                     NSEKFMEQLYTVLKPEKTEVRYQSEWFGKMGMSEVIKLMSKFTLAQFMAHETFRQRYE
                     QGLSLGMHEILYPVLQGYDSVAIESDIELGATEQKFNILCGRDMQRHFGMEQQVAILS
                     PILIGTDGVQKMGKSLNNYIAIFDSPKDKYGKVMSIPDDLILNYYCYATAVPEEEIDK
                     IKLELESGINPMLIKKRLAREIVSLYHSAAETKEAEEAFEKQFSMREIPDDIPEFIIG
                     ESEYKLAKLLVDSKICATNGEAKRLIQGGAVSVNGNKITAFDAIISLQEGDVIKAGKR
                     NYIKIKKI"
BASE COUNT         1115 a          724 c          649 g         1140 t
ORIGIN      
        1 attgcaggtt ccatcaccgg ttctaaattc aaagcgtttt gtttcaggag tattaacata
       61 ttttgggatg cggatagccg cactgcgatt agcgaggcca taaaacagct ttacgggagc
      121 ttcaaaaccg ggcagcaatc ttttataact atttgtggaa ggattagtaa aagccactaa
      181 ggctcgtcca tgtgttaaca cccctccaat aaaccagata gcttcatccg agaggtctgc
      241 atagccaccc tttttgtaaa aaaggttctt accattttta tgtagcatta tatgaaaatg
      301 cattccatta ccagcatggc catataccgg tttaggcata aaagtagctg ttagcccata
      361 ttgtaaagca gttctacgaa ttatatcttt catcaccatt acatcatcac atattttcgg
      421 aaaggcaagt agttctgttt caatttcttc ttgagcagat aggcctactt catgatgatg
      481 atagcgaact ttgatatctt gttcttcaat cagttccaca attttctggc ggatttcgta
      541 aaactgatca aaaggagtat ccatatgata acctttaacc ccttgcaggg caagagcatc
      601 gaggtcttca ttatcttccg gaagagcatc tttacattca ctggaagtaa gattatagcc
      661 agaagaaaaa gcatcggaat aatattgaac cgtatcaaat aaatgaaatt ccagttctgg
      721 aatccaggta gacatatctg caatacccgt agaaagtaaa tatttatgcg ctctgagagc
      781 aacactgcga gggtctttat ttacccctat tctggtttct gcatcgcaaa ttgaacaaat
      841 cattctgaga gttggagttg cattgaatgc atccaactgc gctgttgaag catccggcat
      901 taaaaccata tctccacttt ccacacttgt cattcctggt atggaagaac catcaaaagg
      961 tataccactt tccattactt cagcaatatt acgggcaggg aaagtgatat gataccattt
     1021 accatccaaa ccgcaatatt taagatctac cgctttcacg gcattttctt cgatcatttg
     1081 ttgtaatttc ttcagttcca ttttgtttct cctatatctt tttttttctt atttaggggg
     1141 aaacgatgtt attcatcgtt cacctctaaa cacgattagc ttttgtaaga gcatcggctg
     1201 ctacacaata tcacttaaac aaatacggac attatgagcc ctttgtacct tcccaagata
     1261 taagctgcta ataagttcga tggacacaag tttctcctaa cttacaggga tattaatctt
     1321 tttttatctg ggaatactaa ataatcagca cgcgacattt ttagtgcaca cccataaggt
     1381 tcataataaa attcacaggc ggcactatga tttttccaat aatattccat cccaacaagc
     1441 tgctaattat gataaaggca aaaagataga tcatcccttt tcgttcttgc attgtccatt
     1501 tataataggc ttgatccgtt agaaaaatac ctatcacttt tgatccatcc aatggcggaa
     1561 tagggatcag attaaaaaag gcaagcagca gattgagata tatcacataa gtgcatatat
     1621 attgaactat aaaggagaaa ccgaatagat gatagattaa ggcaaagact atggccacta
     1681 aaaaattgga tagcggacct gccaatcctg ttaaccctga atcagtttta aagttcttaa
     1741 aattataagg attaaatgga accggctttg cataaccgta tataaaaccg caggtaaaat
     1801 ataaaaacaa cggcaggata acggttccaa accagtctat gtgttttaaa ggattcaggg
     1861 ttaagcgtcc agctcttttt gctgaatcat ctcctaaaag aaaagcagcc agagagtgac
     1921 taaattcatg aatgataatg ctgtaaaaga ccagagcgat tataacaaca ttaagtagta
     1981 gtgatattac attcgagtta ttcatatctt ctttattttt atgtaattgc gttttcctgc
     2041 tttgataaca tcaccctctt gaagagatat aatagcatca aaagctgtta tcttatttcc
     2101 attcaccgaa acagctccac cttgaattag gcgtttggct tcgccgttgg tagcgcaaat
     2161 tttactgtcc accagaagct ttgccagttt atattcgctt tcgcctatta taaattcggg
     2221 aatgtcatcg ggaatttcac gcattgaaaa ttgtttttca aatgcttctt ctgcttcttt
     2281 tgtttcagcc gcgctatggt atagggatac aatttcccga gcaaggcgtt tcttaattag
     2341 catcggatta atgccagatt ccaattcaag tttaatttta tctatttcct cttctgggac
     2401 agcagtagca tagcaataat aatttagaat aaggtcatcg gggatagaca taactttgcc
     2461 gtatttatct ttgggggaat caaatatagc aatatagtta ttaagtgatt ttcccatttt
     2521 ctgcacacca tcggtgccga ttaaaattgg tgacaaaata gctacctgtt gttccattcc
     2581 aaaatgacgc tgcatatctc taccgcagag aatattgaat ttttgttccg tagctccaag
     2641 ttctatatca ctttcaatgg caactgaatc gtaaccctgc aaaactggat acagaatttc
     2701 atgcattccc aaggataaac cttgttcata gcgctggcga aatgtttcgt gagccataaa
     2761 ttgagctaaa gtaaatttac tcattagttt aataacctcg ctcattccca ttttaccaaa
     2821 ccactcactt tgataacgga cttctgtttt ttccggtttt agcaccgtat atagctgctc
     2881 cataaacttt tcactgtttt ttatcacttg ttcggaagtt aaaggaggac ggctttcatc
     2941 tcgacctgta ggatcaccta tttgagcagt aaaatcacca attattatta ctccgatatg
     3001 acccaaatct tgaaactgcc tcattttccg gataggaacc aagtgtccta aatgaacatc
     3061 acaaccggta ggatcaatac catattttac tctgagtggt cttcctgtct ttacagattt
     3121 agcgaatttt gcttccagct cttccctggt gataatttct tccacacccc tgctgattaa
     3181 cttcatttct tgttcaaaag ccattgaatc atcctatttt agtaattata ttgtctcagg
     3241 gcaaaaatag tcaacgcata tatttgtcaa gggtaaaggg aattttcttg atggattaaa
     3301 tgaatagctg aaacatttaa gaatatttct atggcggttt atgcactagt ttcggctgct
     3361 gtgagaaatg ttctgtaatt taaaaaacag ggattctagg attagttcac gcaaattgcg
     3421 cagattacgc agatttagtt ttagaaacaa ggatttaagg attaagagga agtcgaatta
     3481 aagttccgct aggaacgata catactaacg acgggttttt aacccggcga caagaagaac
     3541 aaataataaa agtcccagcg ggacgacaca aggatagcga tggtggcgta aataatccgt
     3601 cggctgctgt ctttaagtca aaatcggc
//
LOCUS       DAORHB010000143         3524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_29617_length_3524_cov_4.340156, whole genome shotgun sequence.
ACCESSION   DAORHB010000143 DAORHB010000000
VERSION     DAORHB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29617_length_3524_cov_4.340156"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKM80_04800"
     CDS             <1..603
                     /locus_tag="PKM80_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV93263.1"
                     /translation="FDIQTELFPSANDKHKAEKLLKALSGKTLIAIAPGSIWKTKCWE
                     LNSYISLCRKLLDSGYGIVLIGGESDKPQCDEIEIAIEKNNANLINLAGVTNLLESAA
                     IIEKCSLMICNDSGAMHIANAMQTRVFAFFGPTVPLFGYYPYREGDKVFEVDLKCRPC
                     GSHGAKKCPLDHHNCMKLIAVEPVLEAVNLLFLRRNNEFN"
     gene            590..1333
                     /locus_tag="PKM80_04805"
     CDS             590..1333
                     /locus_tag="PKM80_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNV93264.1"
                     /translation="MNLIDAHCHLANLNQLMPVKPFLEEAKKQGIFFWLSSVLTQSEI
                     AWHQNNPDNRIAYSAGIHPCYDECDLELEDIEILCQKKRIWAIGEIGLDRNNPNYECQ
                     VELFIAQLELAKQYNLPVVLHLVGHTTEAYKILKQYNLQYLVHGYAGSLQGYKELLRL
                     NTLFTISERLLKKDKKELLLAMIDSKRYLFETDITREYVKVGEKNPLLRLSSLVSSVA
                     DLSGIMLEQLITEQQRNFNGYEQSGFFQN"
     gene            1299..2072
                     /locus_tag="PKM80_04810"
     CDS             1299..2072
                     /locus_tag="PKM80_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNV93265.1"
                     /translation="MAMNKADFSRTELLIGEKGLQALSKTCIAVIGLGGVGSYSAEAL
                     ARSGIGKLILVDFDIVEPSNVNRQILALQSTIGKYKADLMQQRILDINPQADIVVHRE
                     VLNKDNQEQLLAEADYCVDAIDSLNSKIDLLEFLVRNKKRFISVMGAGNRLDPNLIHI
                     STLDKSHNCPLARRVRKLLKQREIEINFPCVYSSELPIKPNQKSNNPSIEHPNISQKI
                     VYNNSQRIPIGSISYIPAIMGMMASSWVIKSILKDSTDN"
     gene            2254..3228
                     /locus_tag="PKM80_04815"
     CDS             2254..3228
                     /locus_tag="PKM80_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNV93266.1"
                     /translation="MFLSGLQMKEVFLKAKKMRFGIIASNVVFDTQIRALIQGYAAVN
                     SDGLLQMSTGACKYAAGSSQDIQIGARMISAMAKTFASQFPKCGIGLHIDHATPNYFD
                     FIKFCIENDLVTSVMIDASKENLEDNIRITKEVVDLAHKYNILVEGEIGHIKGAEDEI
                     VSEDELYTKPEEALEFVKRTNVDLFAASVGTNHGVCKGENIVLRLDLIKEIDDLLIKN
                     GVERGLVLHGASGLTDQQQKTAIANGVVKINKDTHYQMDMASAIQEYWEKEKYAIVCP
                     PDMDPNLYVPNKSKFDPRKWLIKGEEKMQNTVMDFCNISGSAQNSILL"
     gene            3245..>3524
                     /locus_tag="PKM80_04820"
     CDS             3245..>3524
                     /locus_tag="PKM80_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNV93267.1"
                     /translation="MTNVAINGFGRIGRLVLRIIINSHPELNVVAINDLTDAKTLAHL
                     FKYDSVHKVYPGQISSEENYLLVNGKKIRIFAEKDPEVLPWKDLGVEYV"
BASE COUNT         1160 a          571 c          689 g         1104 t
ORIGIN      
        1 ttcgatattc agacagagct tttcccctca gctaatgata aacataaagc ggagaaattg
       61 ctaaaggcat tatctggaaa gacattaatt gctattgctc ccggttcaat ctggaagact
      121 aaatgctggg aactaaattc ctatatctca ctttgccgga aacttttaga tagtggatat
      181 ggcattgttt taattggagg agaaagcgat aaacctcaat gtgatgaaat agaaattgct
      241 atagagaaga ataatgctaa cctaattaat cttgcaggag ttactaattt actggagagc
      301 gctgccatta tcgaaaaatg ctctttaatg atttgtaatg atagtggcgc tatgcatatt
      361 gccaatgcta tgcagaccag ggtctttgct ttttttggtc ctaccgttcc tctatttggt
      421 tattatccat atagagaagg agacaaagtt tttgaagttg atcttaaatg cagaccctgt
      481 ggtagtcatg gtgctaaaaa atgtccttta gatcatcata actgtatgaa actcattgcc
      541 gtagaacctg ttttagaggc tgtaaatcta ctttttttaa gaaggaataa tgaatttaat
      601 tgatgcacat tgccatctgg ctaatctgaa tcagttaatg ccagtaaagc catttttgga
      661 agaggcaaaa aaacagggta tctttttttg gctttcatct gtgcttactc aatcggaaat
      721 tgcttggcac caaaataacc ctgacaatag aattgcttac agtgctggta ttcatccttg
      781 ttatgatgaa tgcgatttag aattggaaga tatagaaatc ctttgccaaa agaaaagaat
      841 ctgggcaatt ggtgaaatag gtttggatag aaataatcca aattatgagt gtcaggtgga
      901 gctttttatt gcccaattgg aacttgccaa acaatataat ctaccggtag ttttgcatct
      961 ggtaggacat acaaccgagg cttataaaat cctgaagcaa tataatctgc aatatcttgt
     1021 tcacggttat gcaggttccc tacaaggtta caaagaactt ttacgcttaa atactctgtt
     1081 tactatttct gagcggttat tgaagaagga taaaaaagaa cttttgctgg ctatgataga
     1141 tagtaaacgc tatctttttg aaacagatat cactcgtgaa tatgtaaaag taggagaaaa
     1201 gaatcctctt ttgcgcttat cttcacttgt ttcttctgta gctgatcttt caggaattat
     1261 gttagagcaa ttaattacag aacagcaaag aaactttaat ggctatgaac aaagcggatt
     1321 tttccagaac tgaactttta attggtgaaa aaggactgca ggcattatcc aaaacttgta
     1381 tagcagttat tggtttgggg ggagttggtt cttattctgc agaagcatta gctcgttcag
     1441 gaattggtaa attgatctta gttgattttg atatagttga gccaagcaat gtaaacaggc
     1501 agattttagc tttgcaatca actattggta aatacaaagc agaccttatg caacagcgaa
     1561 ttctggatat caatccacaa gcggatattg ttgttcaccg ggaagttctg aacaaagata
     1621 atcaagaaca gttacttgct gaagctgatt attgtgtaga tgccattgat agtttgaact
     1681 ccaaaattga cttgctggaa tttctggtta gaaataagaa gagatttatc agtgtgatgg
     1741 gagcaggaaa tcgtctggat cctaatttaa ttcatatttc cactttggat aagagccata
     1801 attgtccttt ggcgcgtaga gttcgtaagc tattaaagca aagagagatt gagattaatt
     1861 tcccttgtgt gtattcttct gagcttccta tcaaacccaa tcaaaaaagt aataatcctt
     1921 caattgaaca cccaaatatt tcccaaaaaa tcgtttacaa taactctcaa cgcataccta
     1981 ttggttcaat aagttatata ccagcaatta tgggaatgat ggcttcttcc tgggtcataa
     2041 aatctatcct gaaagatagc actgataatt aataaatcac ctattctgat aaagtagcat
     2101 tttattgagc atcaatactg ctgattgggt attatctctt ccgaaaacag agaatttact
     2161 tttccctttc tgcccttttt tagattttgc ttgacttttt gtggatgata tatagattat
     2221 ctaataagaa ttttattatg ctggaggtat aaaatgtttt tatcaggttt acaaatgaaa
     2281 gaggtttttc taaaagccaa aaaaatgcgt tttgggatta tcgcttccaa tgtggtcttc
     2341 gacacacaaa tccgagccct tattcaaggt tatgcagctg taaattctga tggcttactt
     2401 caaatgagta caggtgcctg taaatatgca gcaggttcct ctcaagatat acaaattgga
     2461 gccagaatga tttcagcaat ggctaagacc tttgcatccc aatttcctaa atgtggaatc
     2521 ggtttacata tagaccatgc cacacctaac tattttgatt tcataaagtt ttgtattgaa
     2581 aacgacttag taacttctgt aatgattgat gcttccaaag aaaatctgga agataatatc
     2641 cgtatcacta aagaggttgt agatttagct cataaatata atattctggt agaaggtgaa
     2701 attggccata tcaagggtgc tgaagatgaa attgtttccg aagatgaact ttatactaaa
     2761 ccagaagaag ctttagaatt tgtaaaacgc acaaatgtag acctttttgc ggcttcagta
     2821 ggaaccaatc atggcgtttg taaaggagaa aatatcgttc tccgcctcga tttaataaaa
     2881 gaaattgatg acctgctaat taaaaatgga gtagaaagag gattagtttt acacggtgct
     2941 tcggggttaa cagaccaaca gcaaaaaact gctattgcca atggagtagt aaaaatcaat
     3001 aaagataccc attatcaaat ggatatggct tctgctattc aagaatattg ggaaaaagaa
     3061 aagtatgcaa tcgtctgccc tccagatatg gatccaaatc tctatgttcc taacaaaagt
     3121 aaatttgacc ctcgcaaatg gctgattaaa ggtgaagaaa aaatgcagaa cacagttatg
     3181 gatttctgca atatttccgg tagcgcacaa aatagtatat tactataact gaaggagata
     3241 gaatatgaca aatgtagcaa ttaacggttt tggacgcatt gggcgtctgg ttttacgcat
     3301 aataattaac agccatccgg aattgaatgt agtggctatc aacgatctaa ccgatgctaa
     3361 aactttggca catcttttta aatacgatag tgttcataaa gtttatcctg gacagatttc
     3421 cagcgaagag aactatttat tagtgaatgg caaaaagatc agaatttttg ccgaaaaaga
     3481 tcctgaagtg cttccctgga aagatttggg agtggaatat gtaa
//
LOCUS       DAORHB010000144         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_29669_length_3520_cov_5.120924, whole genome shotgun sequence.
ACCESSION   DAORHB010000144 DAORHB010000000
VERSION     DAORHB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29669_length_3520_cov_5.120924"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            555..3500
                     /locus_tag="PKM80_04825"
     CDS             555..3500
                     /locus_tag="PKM80_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNV93268.1"
                     /translation="MKRLFSLCLFIVIAFAFGAEEICVRRSDYLELSTFHFSTADGNE
                     IVFWNDTVNGNPDIFAQKLDANGLCLWDENLCLTDSPGDQNIIDLVKTSDNNCIILYT
                     DYSLASVTSYYMQKITPDGQCLWSPNGVLFSCEPMKIDTIKLIPNASGGAHILYTSAY
                     HSLYRQMIDANGSYIFSLDNTSIYTDTLYVTLKNAVSDNSGGAIIAISKYLGLNTTKI
                     THLIHLSQDGFITGNNPLVPNADLYTTNYDILKGIDNQIILWGYCSLPSDGIAFRKIG
                     TNGNLLSPQPTVYQFPSSVSPYDKISPVATLEGGLLLAWQEWNYTMHQYNIKMQKYDT
                     NLLPVWLESGILVSNYGPRENRFQMLMKNNGNILASWTTVNYSPFSIYYMAQLIDPQG
                     IKCWGENGSIIQELSDYLNYVTSPDGSNNIFVWSDTNNNNISIKRNVVTDAGISLLPP
                     EELTIVEHLGRAAMLFDCVALGDHYLNIWFDSRNNNAIYYQIYNSNQEALFQENGIAL
                     TEPNIGLLGILTTEKISENEYAIIYRIQNDDILYATYLQCIDANGTKLYDGNGILLYS
                     TYGGSFSLSGWDNDIYIGWVLNEDDSNSYVMAQRISSGQKMWGEEGKVIAELSNYVNS
                     VKNRGRYFVWSSTDANFLTSIKALKLDVNGNPVSGWNPEGLNLAQLPETFVAHINNAF
                     LEADNLVLTYSYYNFSNYNELYMAQKINSEGELLWSDNGIQICVEDTYPLDTIWDNNN
                     IIFVSSFYYPANNLLFFNIVNSSGELLYGIEGIPINNNSMCCYECSLLKFANGAYLLT
                     CAYFSTEYDCCDIYNIELDPHGNTVANQVICNAIFDQSGVQSSAIGNQAFLAWNDYRT
                     SVFSYDVFITSIYATWVNSSYVANEDEVITNPAVISLQQNYPNPFNPETTISFSLPKA
                     EKVNLNIYNLKGQLVKTLYQDNQCSAGVNSVVWNGKDNKGNSVSSGIYFYRLNCGKEQ
                     LTKKMVLSK"
BASE COUNT         1101 a          614 c          650 g         1155 t
ORIGIN      
        1 cagatccccc catttttaga acagggtagt ataagtcgtt gtgcctcaac ccatttccct
       61 ttatatttat tgatgtagga atttgttaca tagctaaaac aatcggacac cacattagag
      121 atagagaaaa gctaaaaggg atcaaagccc aaaggtaatt tactataacg cttgttgggc
      181 attgcccttt tttttatctt gttcagtgtc atatccattt ctgctccgaa atccttatct
      241 ttccgtctat tcctaccctc aatcttctgc cataattacc ggatgatttt gcgacattgg
      301 caagaatatc ctaattgctt gtcccatagt aaattctcta acaacccagt aacacttccg
      361 taacactgca gttgtctcgt tacttgattg ttacggatat gttactgcgt tataagtgta
      421 atcagggtgc aatgatctgc ttcggtgtag ccgatagata gtttttattt tctttgggaa
      481 aaatatctat ctaatggttc atttcttgca taaatatata tctcattata aaagataata
      541 aaagggagga taaaatgaaa cgcttgtttt ccttatgttt attcatcgta attgctttcg
      601 cctttggggc agaagagatt tgtgttagaa gatctgatta tttggaatta tctacttttc
      661 atttttctac agctgacggc aatgaaattg ttttttggaa tgacacagtt aacgggaatc
      721 cagatatctt tgcgcaaaaa ctggatgcta atggactctg tttgtgggat gaaaacctct
      781 gtctgacaga tagtcccgga gatcagaata ttattgatct ggtgaaaact tccgataata
      841 attgcattat cttatacacc gattattcac tagcttcagt cacaagttat tatatgcaaa
      901 aaataacacc cgacggacaa tgtttatggt ctcccaacgg tgttctattt tcttgtgaac
      961 ctatgaaaat agatacgata aaactgattc ctaatgcctc tgggggcgcg catattttat
     1021 acacttcagc ttatcattct ttatacaggc agatgattga tgcaaatggc agttatattt
     1081 tttctctgga taacacttct atatatactg atacattgta tgtcacgctc aaaaatgccg
     1141 ttagtgataa ttccggggga gcgattattg ctattagtaa gtatttaggt ttaaatacaa
     1201 caaaaataac tcatcttatt catctctcac aggatggttt tataactggt aataaccctt
     1261 tagttcctaa tgcagatctt tatacaacta attatgatat cctcaaaggt attgataatc
     1321 agattatttt atggggatat tgttctctcc caagcgatgg cattgccttt agaaaaattg
     1381 gtacaaatgg taatctgtta agccctcaac ctaccgttta tcagtttcct tcttcagttt
     1441 caccttatga caagatttct cctgtggcaa ctttagaggg aggtttatta cttgcctggc
     1501 aggaatggaa ttatacgatg catcaatata atattaaaat gcaaaaatac gatacaaacc
     1561 tacttcctgt ctggctggaa tcgggaattt tggtaagtaa ctatggtcct agggaaaata
     1621 gatttcaaat gttaatgaaa aataacggca acatcttggc aagctggacc accgtgaatt
     1681 attctccttt cagtatttac tatatggcac agttgattga tccacaggga ataaaatgct
     1741 ggggtgaaaa tggcagtata attcaggaat tatctgatta tctgaattat gttacatccc
     1801 ctgatggcag caacaatata ttcgtttggt ctgatacaaa taacaataac ataagcataa
     1861 aacgcaatgt tgtaaccgat gccggaatta gtttactgcc tccggaagaa ttaactatag
     1921 tagaacactt aggaagagca gcaatgcttt ttgattgtgt agctttagga gatcactatc
     1981 tgaatatctg gtttgattct cgcaataaca acgcaattta ttatcaaata tataacagta
     2041 accaggaggc attattccag gaaaatggca tagccctaac agaaccaaat attggtttgt
     2101 tgggaatcct aactacggaa aagatctcgg aaaacgaata tgccattatt tatagaatcc
     2161 aaaatgacga cattctttat gcaacctact tgcagtgtat tgatgccaac ggcacaaaat
     2221 tgtatgacgg aaacggtatt ttgctgtata gtacctatgg aggatctttt tctttatccg
     2281 gctgggataa tgacatttat atcggttggg tattaaacga agatgactca aatagttatg
     2341 ttatggcaca aagaatatca agcggacaaa aaatgtgggg agaagaagga aaagtaattg
     2401 ctgaattaag taattatgtg aatagtgtta agaatagggg aaggtatttc gtttggtcat
     2461 ctaccgatgc caattttctt acttctataa aagcgttaaa attggatgta aacggtaatc
     2521 ctgtttccgg atggaacccg gaaggattaa accttgctca acttcccgaa acatttgttg
     2581 cccatatcaa taatgccttt ttagaagcag ataatctggt tcttacctat tcctattata
     2641 attttagcaa ttataacgaa ttatatatgg ctcaaaaaat caatagcgaa ggagagttac
     2701 tttggtctga taacggtatt caaatttgcg tagaagacac ctatcccctt gatactattt
     2761 gggataataa taacataatc tttgttagct ctttctacta ccctgccaat aatcttctct
     2821 tctttaatat tgtaaatagt tcgggtgagc ttttatatgg tattgaagga atacccatta
     2881 ataataattc aatgtgttgt tatgagtgct ctttgctgaa atttgccaat ggtgcatatc
     2941 ttttaacctg cgcatacttt tcaaccgaat atgactgttg tgatatatat aatatcgagc
     3001 ttgatcctca tggtaatact gttgccaatc aagttatttg taacgctata tttgatcaga
     3061 gtggtgtgca aagttcagct atcggtaatc aagcatttct tgcttggaat gattaccgga
     3121 cttcagtatt tagttatgat gtgtttataa cctccattta cgccacttgg gtgaatagtt
     3181 cttatgtggc aaatgaagat gaagttatca caaatcctgc cgttatttct ttacagcaaa
     3241 attaccctaa tccctttaat ccggaaacca cgatttcttt ctctctgcct aaagcggaaa
     3301 aagtaaacct gaatatctat aacttaaaag gacaactggt taagaccctt taccaagata
     3361 accaatgttc tgcaggagta aattctgtgg tctggaatgg aaaagataat aaaggaaata
     3421 gcgtaagcag cggcatttat ttctaccgcc taaattgtgg aaaagaacag ctgaccaaga
     3481 aaatggtgct tagtaaataa agtgcgggag ttcaaagttc
//
LOCUS       DAORHB010000145         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_30286_length_3472_cov_6.040094, whole genome shotgun sequence.
ACCESSION   DAORHB010000145 DAORHB010000000
VERSION     DAORHB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30286_length_3472_cov_6.040094"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1122..2171
                     /locus_tag="PKM80_04830"
     CDS             1122..2171
                     /locus_tag="PKM80_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93269.1"
                     /translation="MRKIQIVLILITALIFSACCHNKAKPVTSETENLNYTGSKLNSN
                     YIWGGAMNLAWNELIENFTGEPIELATKDENALKTLHCLNNPVFTKDDIDEASYYVKS
                     GYGQKTVDLINQECRAKFPDKSISDLQLKLADKDIISYAYFLKQIEYEIAFKKQAVNF
                     LDKQVKGFTSDPESYTNVYIVNYENDDKFLLALKLKDNQDQIFLAKGYPMDKPDEVLK
                     MLKTKAPANEKADFIPGTVMNRRDIFQAPVLHLNYERQYKEMLNKKIKNKKLSGYQIA
                     LMQEIIKFDMDEKGARVENEAIIGMITSANPNASKPKNLILDKPYWVIMKRANSNNPY
                     FILGVGDTELMNAAE"
     gene            2194..3360
                     /locus_tag="PKM80_04835"
     CDS             2194..3360
                     /locus_tag="PKM80_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNV93270.1"
                     /translation="MPDPLINAFENALKELNSFSELKKDLRNRNLSNPLKILAIGKSA
                     YPMANVCCDILTTSGIEYSGYLLTKYGNIGAEIPNLVIKEAGHPVPDNNTLQNSQEIL
                     NWLQHIDEKEDLIILLSGGGSSLFEVLENGHTLEDLIALNKTLLQSGVGIKEINMERA
                     KISKVKAGKALNYINAQNIYCYALSDVQNNEPETIASGCFYPTNAIKIDENSYQAILN
                     NNKQQLFYTIVGDNYSLRKQLANFIPPPVYIQPIYFYQNVDIVADYLANFAFSPNQKG
                     LYIFGGEAPVKVTGNGIGGRCTHLALLMAKKIAGKKQITFYALASDGNDNLDNVSGAW
                     VDQNTANKLQNKGIDISCAIKNCDSYTALKTVESIIPAWVQPLNLNDIYLLEIK"
BASE COUNT         1179 a          635 c          625 g         1033 t
ORIGIN      
        1 tttgaactaa gaactcctgc actttgaact aagaactcct gcactttgaa ctaagtacta
       61 agaactcctg cactttgaac taagaacttt gaactcccgc actttgaact aagaacccct
      121 gcactttgaa ctaagaacgt tgaactcctg cactttgaac taagaactcc tgcactttga
      181 actaagaact cctgcacttt gaactaagaa ctcctgcact ttgaacttgt caaacagatt
      241 tcactgataa aataggtctg ggatgaacag gattataagg atttaaggga ttctttatga
      301 agacaagagt tagatgatga agattgtatc tttcactttc cgaggggctt acacctccat
      361 cgctatgatt gtgtcggcct atgtcggcct ttgagttaaa atcggctttg taaattgtct
      421 gccatataat ttcattcttt tttctatcct tgaataaggt caagaatcat atcatagaaa
      481 aggccgattt tgacttaaag tcgactaatt gtcagttaaa aaaatcaatc ctcttaatcc
      541 taaaatcctg ttcagcccaa tttcccgagg ggcttacgcc tccatcgcta tgattgtgtc
      601 ggcctttgag ttaaaatcgg ctttgtaaat tgtctgccat ataatttcat tcttttttct
      661 atccttgaat aaggtcaaga atcatatcat agaaaaggcc gattttgact taaagtcgac
      721 taattgtcag ttaaaaaaat caatcctctt aatcctaaaa tcctgttcat cccaattttc
      781 cgaggggctt acgcctccat cgctatgatt gtgtcgccct aaggggcttt ttttatcaat
      841 tcttcttatc gccgggttaa aaacccgtcg ttaatatgtg tcgttcctaa cggaacttta
      901 aattagaaat cttgtaaatc cttaaaatcc tgttcatctc tgacctattc atttctaatc
      961 tgtgcaatct gtgaaatctg tgagaggata aaatctgcgt aatctgcaaa atctgcgtga
     1021 aaataaatct gtgcaatctg tgagaaccaa gagctaaaca tttgtattga cacaatctcc
     1081 tcggaattta tcctgccaaa aagaatagaa agaggtatcc aatgcggaaa attcaaattg
     1141 tattaatctt aataacagct cttatatttt cggcttgctg tcataataaa gccaaaccgg
     1201 ttacatctga aactgaaaac ttgaattata caggcagcaa actgaatagc aattatattt
     1261 ggggcggagc tatgaatctt gcctggaatg aattaataga gaattttacg ggagaaccca
     1321 tagaacttgc caccaaagat gaaaatgcat taaagacatt acattgttta aataatcccg
     1381 tttttaccaa agacgatata gatgaagcaa gttattatgt caaaagcgga tatgggcaaa
     1441 aaaccgttga tctgataaat caagaatgcc gggcaaaatt tcctgataag agtatatcgg
     1501 acttacagtt gaaattggct gataaagata taatctccta tgcctatttc cttaaacaga
     1561 ttgagtacga aattgctttt aagaagcagg cagttaattt tttggataaa caagtgaagg
     1621 gctttacttc tgatcccgaa agctatacta atgtttatat tgtgaattac gaaaatgacg
     1681 ataaattcct gctcgccttg aaattaaaag ataaccaaga tcagattttt ttggcaaagg
     1741 gctatcctat ggataaaccc gatgaagttc taaaaatgct gaaaaccaaa gctcctgcta
     1801 atgaaaaagc agattttatt cccggaaccg taatgaaccg aagagatata tttcaagcac
     1861 cggttttaca cctaaattat gaacgccaat ataaagaaat gttgaataag aaaatcaaga
     1921 acaaaaagct ttctggttac caaatcgctc tgatgcaaga aattatcaaa tttgatatgg
     1981 atgagaaagg tgctcgcgta gaaaatgaag ctatcatcgg aatgatcact tccgccaatc
     2041 ccaatgcctc taagcccaaa aacctgattt tggataaacc ttattgggta ataatgaaaa
     2101 gggcaaattc caataacccc tattttattc tgggagttgg tgatacagaa ttaatgaacg
     2161 ctgccgaatg aacaaaatat gtttaactaa tatatgccag acccattgat caatgccttt
     2221 gaaaatgccc tgaaagaact caattccttt tcagaactaa aaaaggactt aaggaataga
     2281 aatttatcca atcctcttaa aattttagcc atcggaaaaa gcgcttatcc gatggctaat
     2341 gtttgttgcg atatccttac tacaagcgga atagaatact ccggctattt gctaaccaaa
     2401 tatggaaata ttggtgctga aataccaaat ttagtgataa aagaagctgg tcatcctgtt
     2461 cctgataaca atactcttca aaatagccag gaaattctta attggctgca acatattgat
     2521 gaaaaagaag atttgattat tttgcttagc ggaggtggaa gttctttatt tgaagtgctg
     2581 gaaaatggac atactttgga agacctgatt gctctgaata agacattgct acaaagcgga
     2641 gtaggaatca aagagataaa tatggagcgc gccaaaatat ccaaagtgaa agccggaaaa
     2701 gcactaaact atataaatgc acaaaatata tactgttatg ccctatccga tgtgcaaaat
     2761 aatgagccgg aaactattgc ttcgggctgt ttctatccaa caaatgccat aaaaattgat
     2821 gaaaatagtt accaggcaat tttgaataat aataaacaac agcttttcta tactattgtg
     2881 ggagataact attctctgcg taagcagtta gctaatttta ttcctccacc cgtatatatt
     2941 cagcccatat atttttatca gaatgtggat atagtggcag attaccttgc taattttgct
     3001 ttttccccaa accaaaaagg tctgtatatc tttggtggag aagcacctgt aaaagttaca
     3061 ggaaatggaa ttggcggtcg ttgcactcat cttgctctgc tgatggcaaa aaaaatcgct
     3121 ggcaaaaaac aaataacttt ttatgctctt gccagcgatg gaaatgataa tttggataat
     3181 gtttcaggtg cctgggttga tcaaaacaca gctaataaat tgcagaataa agggatagac
     3241 atctcgtgtg ctattaaaaa ttgtgattcc tacacagccc taaaaaccgt tgaatcaata
     3301 atccctgctt gggttcagcc cctcaattta aatgatatct acctcctgga aataaagtaa
     3361 gtacgcagat tttaggattt cgaaacgagg attttaggat taagaaacga ggatttaagg
     3421 attttaggat taagaggttt tttattatta gccaccgaag acaccgaaaa aa
//
LOCUS       DAORHB010000146         3327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_32330_length_3327_cov_4.641198, whole genome shotgun sequence.
ACCESSION   DAORHB010000146 DAORHB010000000
VERSION     DAORHB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32330_length_3327_cov_4.641198"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PKM80_04840"
     CDS             <1..1184
                     /locus_tag="PKM80_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93271.1"
                     /translation="DLWKKDAEGEGYKIYSGGMVSMGEDEVGAVDKLWILPPGSYLVK
                     LGGGSWSDLKDFATVALAEGDKKTLTMIVDPAAEGNVLIGAGVFADEDIGLGSKRIHR
                     GAIHGNISLSSNNNVDKKKPTSSVSLAGQFDNSIDTHELIKPFHFTTRSIYDLGLNKT
                     SNQDLIFSPDDYSLKSVLMLYPMEKKKFLRNFAFYGRMNINTHFFDETTYFSENKNII
                     LQNAEGDICSTLLDQPDLKTKIAFYPLRLKEGTGLTYQINFSPRAWTSLRVGYGWLQD
                     YNTSSYVFDKTIVDTQTGLSYDRYIEEPNSSSKGIESTIIFSALNLLKFVSINSTLDV
                     LFRMGVPDHTYRLENENRISFKLFRNISLDVKFNTFYDEAEKPWMVYDYTTFLRLSLF
                     Y"
     gene            1193..2269
                     /locus_tag="PKM80_04845"
     CDS             1193..2269
                     /locus_tag="PKM80_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HNV93272.1"
                     /translation="MPCRIVNNTLYLEGNLTKETVPEVLYRINTLLKNQSPNTIDLSG
                     ISNLDSAGVAFLDELHNRLAAEGLLMFQGVQPQIQEVIDTFTSLKIETPIPARKMGFF
                     EKIGDSVVLAAQNFYQVMMLASEVFYWSLIGIFNRKGQRKGSFIIQCSLLGSQALPIL
                     ALLSFIVGFILALQASVQLANFGANVFIADLMAFSMEREIAPLITAIIVAGRSGSAIA
                     SEIATMQVTEEIDALKIMALSPVRYVVVPKFHAITVVMPILVMFSILVSEIGGGIIAI
                     DYLDLSVYTFVQRSIDVLSMKDIVTSFGKSIVFAWTIVVIGAYCGFEVKGGAEGVGKA
                     TTSSVVASIFAVIIWDAIFSLLYL"
     gene            2272..3009
                     /locus_tag="PKM80_04850"
     CDS             2272..3009
                     /locus_tag="PKM80_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV93273.1"
                     /translation="MEPIISVKNLVAKYGDNIVLDNISVDIFPGEITVILGGSGCGKT
                     TLLKNILRLVEPFSGSVKIWGEEVLDLDDIQMANILKKIGMLFQSGALLNSISVYDNI
                     SIPLELHTDLSQNLIDRIIKVKLNLVNLSEALYKFPSELSGGMKKRAALARAIALDPQ
                     ILFCDEPSAGLDPLTAASLDELILELKKQLKMTIVVVTHELASIHRIADKIIFLDSGK
                     MLFNGTLEDAKNAGIPQIDTFFKVGKF"
     gene            3045..>3327
                     /locus_tag="PKM80_04855"
     CDS             3045..>3327
                     /locus_tag="PKM80_04855"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNV93274.1"
                     /translation="MIGKNGKLPWDIPEDLTRFKKITTGYPVIMGKTTWKSIGKALPG
                     RQNIVISKDVSLQLEGAIVVHSIEEALKLVDGQEAFVIGGAQIYKQFLPL"
BASE COUNT         1007 a          519 c          700 g         1101 t
ORIGIN      
        1 atgatctctg gaaaaaagat gcggaaggtg aaggttataa aatttatagt ggagggatgg
       61 taagtatggg cgaagatgaa gttggagccg tagataagct ttggatttta cctccaggtt
      121 catatttagt aaaattgggt ggtggctctt ggagtgattt aaaagatttt gccacagttg
      181 ctttagcaga aggcgataag aagacgctaa caatgattgt tgatcctgca gccgaaggaa
      241 atgtattaat cggtgcaggt gtttttgctg atgaagatat cggtttgggt tccaagagaa
      301 ttcatagagg agccatacat ggtaatatat ccttatcttc taataataat gtagataaga
      361 aaaagccaac ttccagcgtt tcacttgccg gtcaatttga taacagtata gatacacatg
      421 aacttataaa accctttcat tttactactc gaagcattta tgatctgggc ttaaataaaa
      481 catccaatca agatctgata ttcagtccgg acgattattc tctgaaaagc gtgttgatgt
      541 tatatcctat ggaaaagaag aaattcctgc ggaatttcgc attctatgga agaatgaaca
      601 taaatacgca tttttttgat gagacaacct atttttccga aaacaagaat ataatacttc
      661 aaaacgcaga aggtgatatt tgttcaaccc ttttagatca accggattta aaaacaaaaa
      721 tcgctttcta ccctctgcgt ttgaaagaag gaaccggctt aacttatcaa ataaatttca
      781 gccccagagc ttggacaagc ttacgagttg gttatggttg gctgcaggac tataatacaa
      841 gcagctatgt tttcgataaa accatagttg acacccaaac cggattgagt tatgatagat
      901 atattgaaga accgaacagc agttctaaag ggatagaatc taccatcata ttttctgcct
      961 taaatttact taagtttgta agcataaatt ctactttgga tgtgcttttc aggatgggtg
     1021 tgccagatca tacttatcgt ttggaaaacg aaaatcggat tagctttaaa ttatttagaa
     1081 atatctcgtt agatgtaaaa ttcaatactt tttatgatga agcagaaaaa ccctggatgg
     1141 tttacgatta tactactttc ttacgccttt cgttatttta ttaagaggat taatgccctg
     1201 tagaattgta aataacacac tttatctgga aggaaacctt actaaggaaa cggttcctga
     1261 ggtcttatat agaattaata cactccttaa aaatcaaagt cctaatacga tagaccttag
     1321 tggaatatca aatttggata gtgcaggtgt agcttttctg gatgaattgc ataatcgttt
     1381 agcagctgaa ggattattga tgttccaggg cgttcaacca caaattcaag aggttataga
     1441 tacttttacc tctttaaaaa tagaaactcc tatcccagca cgcaaaatgg gtttctttga
     1501 aaaaataggt gattctgttg ttttggcagc tcagaatttc tatcaggtaa tgatgttggc
     1561 ttcagaagta ttctattgga gcttaattgg tatttttaat agaaagggac agcgcaaagg
     1621 ttcctttatt attcagtgtt cactcttggg ctcacaagca ttgccgattt tagctttgct
     1681 atcttttata gtaggtttta tattagcatt gcaggcctcg gtgcagttag ctaattttgg
     1741 agcaaatgtt tttattgccg atttaatggc tttttctatg gagcgagaaa tagctcctct
     1801 aataacggct attattgtag ctggaagaag tggttcagct atagcttcag aaattgctac
     1861 aatgcaagtt acggaagaaa ttgacgcttt gaagattatg gctctttctc ctgtgcgcta
     1921 tgttgttgtc cctaaatttc atgctatcac agttgttatg cctattctgg ttatgttttc
     1981 tatactggtt tctgaaattg gaggcggtat aattgctatt gactatttag acctcagtgt
     2041 ttatactttt gtgcagcgtt ccattgatgt tctatccatg aaggatatag taaccagttt
     2101 tggtaagagt attgtttttg cctggacgat tgttgtaatt ggtgcctatt gcggttttga
     2161 agttaaaggt ggagctgaag gagttggtaa ggcaactact tcttcggttg tagcttctat
     2221 ctttgctgtt attatttggg atgccatttt cagtttgttg tatttataat tatggaacct
     2281 ataatcagcg ttaagaactt ggttgctaaa tatggtgata atattgttct ggataatatt
     2341 agcgtggata tctttcctgg tgagataact gttattttgg gggggagtgg atgtggaaaa
     2401 accactcttt tgaagaatat tttgcgcttg gttgagccct tttcgggtag cgtaaagatt
     2461 tggggagaag aggttttaga tttggatgat atccaaatgg ccaatatttt aaaaaagata
     2521 ggaatgctat ttcaaagcgg ggctttgctt aattctatct ccgtttatga caatattagt
     2581 atccctctgg aattgcatac tgatctttcc caaaatttaa tagatagaat aattaaagtg
     2641 aagcttaatc tcgttaacct cagtgaggcg ctttataaat tcccctcaga actttccggc
     2701 ggtatgaaaa aaagagctgc tttggcaaga gcgattgctt tggaccctca aatcctgttt
     2761 tgtgatgaac cttctgctgg attggatcct ttaactgctg cttccttaga tgaattgatt
     2821 ctggaactaa aaaaacaact gaaaatgacc attgtggttg ttactcacga attggcttcc
     2881 atacatagaa tagccgataa aatcattttt ctggatagcg ggaaaatgct ttttaacgga
     2941 actctggaag acgcaaaaaa tgcaggtatt cctcaaattg ataccttttt taaagtaggt
     3001 aaattctgag tatctctatt atagtcggtt ttgatttaaa tcgggtgatt ggtaaaaatg
     3061 ggaaattgcc ctgggatatt cctgaagatc ttaccagatt caagaaaata acaacaggtt
     3121 atcctgtaat tatgggaaaa acaacctgga aatctatagg aaaagcacta ccgggtaggc
     3181 aaaatattgt tatcagtaaa gatgtatctc tccaattaga gggagccatt gttgttcata
     3241 gcatagaaga agccctgaag ctggttgatg gacaagaagc atttgtaata gggggcgctc
     3301 aaatttataa acaatttcta cctttgg
//
LOCUS       DAORHB010000147         3303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_32671_length_3303_cov_5.598214, whole genome shotgun sequence.
ACCESSION   DAORHB010000147 DAORHB010000000
VERSION     DAORHB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32671_length_3303_cov_5.598214"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PKM80_04860"
     CDS             complement(<1..880)
                     /locus_tag="PKM80_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV93275.1"
                     /translation="MESENGKDLNSENVSEELLLSQLEFVAIDIETTGLMPARDEIIQ
                     IAAFRFKGNEVVDSYVSFVKPKGKVPKFIEQLTHISPEELKLSPPLKEVLKEFCDFIG
                     NTPLVGHNINFDLSFINQSLVENGSFPLINVFWDTMEIARMYLPFTTDHKLSTMVSFF
                     GIKLDNAHRADSDALATGYLFNALTNYALAHFNYMLNARLQDLSLQANVESGLTEYLR
                     WLVKQQRATVLSGKKPIPPKNELNNVIEHNVSGSIDSIPEIFAEHGLFANKFPNFEFR
                     SGQMVMAEQVSNAFAKQ"
     gene            complement(898..>3303)
                     /locus_tag="PKM80_04865"
     CDS             complement(898..>3303)
                     /locus_tag="PKM80_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV93276.1"
                     /translation="QRLLAEPYNITNIYNNIGVTLMDHNEYDRAEDALKKSLEIANEF
                     GFQRNTIASLNNLGGLYFKKGDWAESISYYEKAIKLSQESGFNEGLLRSFNNLGEVYE
                     KQDELNLAYDLYIKGLELLPAVSDDYIKAELYGNLGSVLTKLHKFKEAYRYLVESLDF
                     FKNIAARDKIIEGYLKQAYYFILTHNVESADYYLSEAQKLAAEQNLPFEIGRAYYLRA
                     VLERKNLEAAKNYLNEAIKLFVEVGSNYELSLVNYELAGVLLDMEEWEQALQILKNNR
                     KIIEKYGSIKLLEQNDILMQKISREYASQMEEAKFEENLLNQFYEITQKLNTITDLDV
                     LIEQSLTSLIEIANADGGVLLLHANPNLPDAWEYKIYNNFSPDDKYHDHFLNICADVY
                     QNNKLENIKQPHFAPSYNNILVLPLSIRKNTMGVILIFCKSGSKYFSERIINLLSALA
                     NQIIVIIDNIRSANLEKSHAIIREQLTSNNIYADIIGKSPEMMKIFEIIEKVKDTPTT
                     VLLEGPSGTGKELIARALHYSSNRRNKAFVAQYCGALPETLLESELFGHVKGSFTGAA
                     YDKKGLFEIADGGTFFLDEIADISQSTQAKLLRFLQEGEVKRVGATKTEKVNVRVVCA
                     TNVPLMERVKRGDFRLDLYYRLNVIRIDVPQLKNRTGDIPLLAIHFLDKYTKRMDKNV
                     LGFTKEAMDILEQFDWPGNVRQLENEIERAVTLVEPNTFIKPSDFSEEVFHHIEYSKT
                     IAMLSGKKSLKEAIEELERKIITQSLEKFQWNQTQAAKELGLSRQGLIKKMQRYNLYK
                     EED"
BASE COUNT         1003 a          637 c          521 g         1142 t
ORIGIN      
        1 cctgtttagc aaaggcatta gatacctgtt cagccataac catttgtccg gaacgaaatt
       61 cgaaattagg aaatttatta gcaaaaagtc cgtgttctgc aaatatttct ggaatgctat
      121 ctatagaccc agagacatta tgttcaatta cattgtttaa ttcattcttg ggaggaatgg
      181 gttttttccc tgataaaaca gtggcacgct gttgttttac tagccatcta agatattcag
      241 ttaaaccact ttctacattt gcctgtagac ttaaatcctg caaacgggca ttaagcatat
      301 agttaaagtg tgccaaagca taattggtaa gagcattaaa aagatatccg gtagctaaag
      361 catcagaatc agcacgatga gcattatcca atttaatacc aaaaaaagaa accatagtac
      421 ttaatttatg gtcagtagta aagggtaaat acatcctcgc tatttccata gtatcccaaa
      481 acacattaat taatggaaaa cttccatttt ctactaaaga ttggttgata aaggataggt
      541 caaaattgat attgtgtcca actaaaggag tgtttccaat aaaatcgcaa aattccttaa
      601 gtacctcttt taatggagga gataatttta attcctcagg ggaaatatga gttaattgtt
      661 caataaattt aggaaccttg cctttgggtt ttacaaagct aacatagcta tcaacaactt
      721 cattgccttt aaaacggaaa gctgctattt ggattatttc atctcgcgct ggcattaaac
      781 ctgtggtttc aatatctatt gccacaaatt ctaattgcga gagaagcagt tcttcgctta
      841 cattttctga gtttaaatct ttaccgtttt cgctttccac tatctttttc ccgtaagtta
      901 atcttcttcc ttatagaggt tatatcgttg catctttttt atcagcccct gacgagatag
      961 ccctaattct tttgcggctt gagtttgatt ccattgaaat ttctctaggg attgagttat
     1021 tattttccgt tccagttctt ctatggcttc tttcaatgat tttttacctg acaacatagc
     1081 gattgtttta ctatattcaa tgtgatgaaa tacttcctca ctaaaatcac ttggtttaat
     1141 aaaagtattt ggttcaacaa gggttactgc tcgttcaatt tcattttcca actgacgcac
     1201 attcccaggc caatcgaatt gttctaaaat atccattgcc tccttagtaa agcccagaac
     1261 atttttatcc atccgttttg tgtatttatc taagaaatga atggccagta atggtatatc
     1321 accagtcctg tttttaagtt gaggtacatc aatccggata acatttaaac ggtaatatag
     1381 gtccaaacga aaatcccctc gttttaccct ttccattaaa ggaacattcg ttgcgcaaac
     1441 tactcgtaca ttaacttttt ccgtttttgt agcgccaact cttttaactt caccctcctg
     1501 taaaaatcga agcaattttg cttgagtaga ttgacttata tcggcaattt catctaagaa
     1561 aaaagttcct ccatcggcaa tttcaaagag gcccttttta tcataagcag cacctgtaaa
     1621 agaacctttc acatgaccga atagttcgct ttctaataaa gtttctggta aagcaccaca
     1681 atattgagct acaaatgctt tgtttcttcg attgctgcta taatgtaaag cacgagctat
     1741 tagttctttt cctgttccac ttggtccttc caaaagaact gttgtaggag tgtccttcac
     1801 tttttcaata atctcaaata ttttcatcat ttcagggctt ttaccaatta tatcagcata
     1861 tatattattg gaagtgagct gttctcgaat tattgcatgg cttttttcca gattggcact
     1921 acgaatatta tcaatgatta caataatctg gttggcaagg gcagagagaa gattaatgat
     1981 tctttccgag aaatacttgg aaccgctttt gcaaaaaatg agtattaccc ccatagtatt
     2041 tttacggatg gatagaggaa gcaccaaaat attattataa ctgggggcaa agtgaggttg
     2101 cttaatattt tccagtttat tgttctgata tacatcagcg catatattta agaaatgatc
     2161 gtgatattta tcgtctggcg aaaaattgtt ataaatttta tactcccaag catcaggcaa
     2221 attgggattc gcatgaagta acagaactcc accatctgca ttggcaattt caatcaagct
     2281 ggtaagagat tgctcaatta gcacatccaa atcagttatt gtattcaact tttgagtaat
     2341 ttcataaaat tggttcagca ggttttcttc aaacttggct tcttccattt gcgaagcata
     2401 ttcgcgcgaa attttttgca ttagaatgtc gttctgttcc agcagtttta tggagccata
     2461 tttctctatt atcttcctat tatttttcaa aatctgaagt gcctgctccc attcttccat
     2521 atccaaaaga actccagcaa gttcataatt aactaacgat agttcatagt tgctaccaac
     2581 ttctacaaat agcttaattg cttcattaag atagtttttt gcggcttcta aatttttcct
     2641 ttccagcaca gcacgtagat aataagctct acctatttcg aaaggcaaat tttgttctgc
     2701 agccagtttc tgagcttcag aaagataata atcggcactt tccacattgt gagttaaaat
     2761 aaagtaatat gcttgtttta gatagccctc gattattttg tcacgagcag caatattctt
     2821 gaagaaatcc aaactctcta ctaaatatct ataagcctct ttaaatttat gcagcttagt
     2881 taaaacacta cctaaattcc catatagctc tgccttaata taatcatctg atacagcagg
     2941 taacaattcc aagcctttaa tatagagatc ataagcaaga tttaattcat cctgtttttc
     3001 gtagacctca cccagattgt tgaaacttct taagaggcct tcattaaaac cgctttcctg
     3061 tgagagctta atcgcttttt catagtaaga tatcgattca gcccagtctc cttttttaaa
     3121 atacaagcct cccaaattat ttaaagaagc aattgtattc cgttgaaaac caaattcgtt
     3181 tgctatctcc aaagattttt ttaaagcatc ttctgcgcga tcatattcgt tgtgatccat
     3241 cagtgtgaca ccaatattat tatagatatt agttatatta tatggttcgg caagtaatct
     3301 ttg
//
LOCUS       DAORHB010000148         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_32848_length_3292_cov_4.200803, whole genome shotgun sequence.
ACCESSION   DAORHB010000148 DAORHB010000000
VERSION     DAORHB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32848_length_3292_cov_4.200803"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKM80_04870"
     CDS             <1..972
                     /locus_tag="PKM80_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNV93277.1"
                     /translation="FAEQRDGVIAPVVFELLGKGRELADELSAELSAVLLGHQIESLS
                     SELIAFGADQVIIVDDPALKHFRDEFYAKAFTELALKYKPSVTLAGATVIGRSFIPRV
                     SINLHTGLTADCTGLSIDPDTGDLLQTRPAFGGNIMATIRTANHRPQMATVRHKVMNP
                     IPRDDSRNGIVIQEQVNFDLDEERTVWLGFEKEKTNLINITEANIIVSGGRGLKESKN
                     FALIEELANALGGAVGASRAAVDSEWIAYSHQVGQTGKTVKPLIYIAVGISGAIQHLA
                     GMSSSDYIIAINKDPDAPIFKVADLGIVGDLFEIVPKLTKRIKEQRV"
     gene            969..2180
                     /locus_tag="PKM80_04875"
     CDS             969..2180
                     /locus_tag="PKM80_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV93278.1"
                     /translation="MTIRPSISVINPDRVEQGVDLHLWGTPFQELELHQDFPDAIAYI
                     PLINSHDHLIGNWVPRAGDHRPYQNSHIWVEDMKDSFAFRERDLFWNNDGNFHLEEPN
                     ALLLAQLGAYKNLFCGCSVVQDHSPLQVDAYYKAMPIIVTKNFRQCHSITLGNWWGGE
                     NPQKEMQLTQGKMPFIVHLAEGIDEVSKLEFAEVRKLGLLKENSLFIHCIALQEDEIA
                     DIAKAGASICWCPSSNFYLIGDTLKIEAALANKVNVCIGTDSTMSGGINLIAEFLTIR
                     EHFPFIPVKEIYRMATENAAKALYLGKDYASINPDKCNNLLLVDKIDRDPFENLYTME
                     ASTIQLLLVDGIPRFGDSEWMELLNLDEDKYTTFRTGNKEKFVIDDPIDLNEQIDEVL
                     GYHKDFPYLPF"
     gene            2343..2972
                     /locus_tag="PKM80_04880"
     CDS             2343..2972
                     /locus_tag="PKM80_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV93279.1"
                     /translation="MEKHLNVTDIFYSLQGESTYSGYPCIFIRLSECNLRCSYCDTQY
                     AFGKGKSMAISSILEQIKKYPCSLTEITGGEPLFQEDIDALFEALHNAKYDILLETNG
                     SISINNVPDYVIKIVDVKTPGSGMENSFLLANLQFLNTQDELKFVITDKVDYKFALSF
                     IKQHNPSVKTIHFSPVTEVLNASELSKWMLEDGVKAKLTLQLHKIIGLE"
BASE COUNT         1004 a          534 c          669 g         1085 t
ORIGIN      
        1 tttgctgaac agcgtgatgg agtaatagct cctgttgttt ttgaactttt aggaaagggt
       61 cgcgaacttg ccgatgaact tagtgctgag ctttcagctg ttcttttagg acatcagata
      121 gaatcgttat catccgaatt aattgctttt ggagcagatc aagttattat tgttgatgat
      181 cctgctttaa agcattttcg tgatgagttt tatgctaaag cttttacaga gcttgctctt
      241 aaatataagc cctcagtaac tttagctggt gcaacagtta ttgggcgtag ttttattccc
      301 agagtatcaa ttaatttaca tactggttta actgctgatt gtacaggcct ttccatagat
      361 cctgatacag gcgatttatt acaaacgcgt cctgcttttg gtggaaatat aatggctact
      421 ataagaacag ctaatcatcg tcctcagatg gctactgtcc gtcataaagt tatgaatcca
      481 atccccagag atgattctcg caatggaatt gtaattcaag aacaggttaa ttttgatttg
      541 gatgaagagc gaactgtttg gcttggtttt gaaaaagaga agaccaattt aatcaatatc
      601 actgaagcca atattattgt ttccggaggg cggggattga aagaatccaa gaattttgcc
      661 ttaattgaag agctggcaaa tgctttaggt ggagcagttg gagcttccag agctgctgta
      721 gattcagaat ggatagcata ttcccatcaa gttggccaga caggaaaaac tgttaaaccc
      781 ttgatatata tcgctgttgg aattagtgga gcaatacagc accttgccgg aatgtccagt
      841 tctgattata ttattgctat caacaaagat cctgatgcac ctatattcaa agtagcagat
      901 ttaggtatag ttggagatct ttttgaaata gttcccaaac taactaaaag gataaaagaa
      961 caaagagtat gactatcagg ccttcgatca gtgttatcaa tcctgatcga gtggaacagg
     1021 gagttgattt acatctttgg ggaactcctt ttcaggagtt agaattgcat caggattttc
     1081 ctgatgcgat tgcttatatt cctcttatca attctcatga ccatttgatc ggtaactggg
     1141 ttccaagagc aggagatcat cgtccctatc aaaattctca tatttgggtg gaagatatga
     1201 aagattcttt tgcttttcga gaacgcgatc ttttctggaa taatgatgga aactttcatt
     1261 tggaagaacc caatgctctt cttttagcac aactgggtgc gtacaaaaat ttattttgtg
     1321 gttgcagtgt tgttcaagat cattctccgc ttcaagttga cgcctattac aaagcgatgc
     1381 caataatagt taccaaaaat ttcagacagt gtcattctat aactcttggt aattggtggg
     1441 gcggagaaaa ccctcaaaaa gaaatgcagc ttacccaagg taaaatgcct tttattgttc
     1501 atttagcaga aggaattgat gaagtaagca agttagaatt tgcggaagta agaaagcttg
     1561 gtctgctgaa agaaaattcg ctatttattc attgtattgc tttgcaggaa gatgaaatag
     1621 ctgatattgc caaagctgga gcttcaatat gttggtgtcc ttcctccaat ttttatttaa
     1681 ttggtgatac tttaaaaatt gaagctgctc ttgccaataa ggtaaatgta tgtatcggaa
     1741 cagattccac tatgtctgga ggaataaatc tgatagcaga gtttttaacg attagagaac
     1801 attttccttt tatacctgta aaagagattt atcggatggc aacggaaaat gcagctaaag
     1861 ctttatacct gggaaaagac tatgcttcta ttaatcccga taaatgcaat aatttattgt
     1921 tggtggataa gattgaccgc gatccttttg agaatttata cacaatggaa gcttctacta
     1981 ttcagctttt attggtagat ggtattcctc gttttgggga tagtgaatgg atggaacttc
     2041 taaatttgga tgaagataaa tatactactt tccgaaccgg caataaagaa aaattcgtga
     2101 tagatgatcc gatagacctg aatgagcaaa ttgatgaagt tttgggatat cataaagatt
     2161 ttccctattt acctttttag ggagttctta cttttgttcg atcccggaaa ttactaaagg
     2221 tttgttcatt tccgttctac catatctggg aaataagggt tgtctttatt tttgaataac
     2281 catcgagatt tggttgttct cattttatca tatttctgct gaaatataat aaggataaac
     2341 aaatggaaaa acatttgaat gtaacggata ttttctattc tttacaggga gaatcaactt
     2401 acagtggtta tccctgtatt tttatccgct tatcggagtg caatttacgg tgttcttact
     2461 gtgatactca atatgccttt ggcaaaggta aatcgatggc tatcagttcc atacttgagc
     2521 agataaaaaa atatccttgt tcgctgactg aaataactgg aggagaacca ctttttcagg
     2581 aagatataga tgcattattt gaagctttac ataatgccaa gtatgatatt ttgctggaaa
     2641 ccaatggttc aatttctatt aacaatgttc cggattatgt tattaaaatt gttgatgtta
     2701 aaacccctgg cagcgggatg gaaaatagtt tcttattagc aaacctacaa tttttgaaca
     2761 cacaggatga actaaaattt gtaataactg ataaagtgga ttataagttt gctttatcct
     2821 ttataaagca gcataatcct tccgtgaaga caattcattt ttccccagtt acagaagttc
     2881 taaacgcttc agagctttca aaatggatgc tggaggatgg agtgaaagcg aaattaactt
     2941 tacaattaca taaaattata gggttagaat aagacataat tttgctgttt ttctacttac
     3001 ttcctggtta gtagctatcg tggtattgtg gtgctgctgc tgggcaaaat ccccagaagc
     3061 tctattttat ctccaccgta tgtaactaag ggggaagaag gttttactgc tttgttttgg
     3121 ttgattcccc gattttacga ataattatag ttcaataagc atacaagggg ataatagggc
     3181 tacacttagt agctgcagca agagtatcta acaaaacaag ttatagatga agcctttctt
     3241 tatcagccac cgctgattaa gtgaataact tttaaatcgg cattttgagg aa
//
LOCUS       DAORHB010000149         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_33043_length_3279_cov_4.517370, whole genome shotgun sequence.
ACCESSION   DAORHB010000149 DAORHB010000000
VERSION     DAORHB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33043_length_3279_cov_4.517370"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKM80_04885"
     CDS             complement(<1..286)
                     /locus_tag="PKM80_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HNV93280.1"
                     /translation="MSNQTKAYIFAGCSIIAWSTISTAFKLSLQFLTPIGLLFFASCS
                     AVLFLNITQSIRNPSFYRVKGLLARILKNLKASFLSGLLNPFVYYLMLFEA"
     gene            complement(416..1501)
                     /locus_tag="PKM80_04890"
     CDS             complement(416..1501)
                     /locus_tag="PKM80_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV93281.1"
                     /translation="MKKVLFLLTALLLVLTACSTARQKLSPQGNVNLKTADVYYSQEN
                     VDQAEIYYLKVLEDNPDHVIALRRLGDISLFKAENFTAREVEFYEDAFEYYSKAISVT
                     ENFPELTNQDRIDLRDMRKRKERAWARIYMAAEKEKEAGNTQKAKEIFELAHKLEPDR
                     PEPMIQLKNIYLVDIKDEVKAEQILKQLLQKDPDKLEYLLEMGTFYYNKGNYAEAVKY
                     FDHARVQNPTNVDNLMNISACYYELKDYEKAMIATKTALEIEPNNLDLLENARSIADQ
                     MNDLDQSIEYLKRILDIRPNEDTYSILAAHLMQKQDWQQLIKYAEEWYNWDTTNKIAV
                     EYIIWAAQQSGNRLLATKYSEIKNKMP"
     gene            complement(1606..2391)
                     /locus_tag="PKM80_04895"
     CDS             complement(1606..2391)
                     /locus_tag="PKM80_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNV93282.1"
                     /translation="MHNIILTPLDYLIVLPFIFLAGLIDSIAGGGGLISLPAYWSVGI
                     PPHLALGTNKFSSCCGTLFSTANYFKARMIDIPVAVISAGFALIGSWLGASSALKVSP
                     AVLNYLLIFLIPAVAVFSLLKRNMGMQSTAQLLAKSYRLIISAITGLGIGFYDGFFGP
                     GTGTFLLLIYTSLLHYDFVTGNGNTKVVNLASNLAAVITFAFAANIYYPLAIPGAICG
                     IVGNVVGSKLVIARGNKLIKKVFILSLVLLFTRILYNLAFSKT"
     gene            complement(2395..>3279)
                     /locus_tag="PKM80_04900"
     CDS             complement(2395..>3279)
                     /locus_tag="PKM80_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93283.1"
                     /translation="DSTGVSVSNGDYDWVYITSPTGSLSSEINCLLNIYLKPGNISSN
                     NLLKCLQSDTSVNLIEHNSKRNNWQSYFFLPGSVYNDESTIYGSQNIQEVLWIDLVRN
                     KITSNLQFSMERTLDKRYQTPDRTYSFLQLAQFDIKGYSEYNTRLQFSKENNQDTRYL
                     NESEILSINAMIQKNLNLQSNIQAEIIYSSESGGRQDGSENYTLQSISLSPTMKSVFM
                     QKYRLTAKLGVTYNKLSGSDYFTFLPQKRAGWIPSASISGVYRINSFSVINLDYRYSD
                     YPKQRDTQELKLEFKAEI"
BASE COUNT         1075 a          590 c          526 g         1088 t
ORIGIN      
        1 atgcctcaaa aagcattaaa taataaacaa atggatttaa caaaccagaa agaaaagatg
       61 ctttcagatt cttcaaaatg cgagctaata atccttttac tcgatagaaa gaaggattcc
      121 tgatgctctg ggttatattt aaaaaaagta cagcactaca agaagcaaag aacaataaac
      181 caataggggt tagaaattgt aaagatagtt taaaagctgt gcttatcgta gaccaagcaa
      241 ttatagaaca accagcaaat atatatgctt tagtttgatt tgacattaaa cagagtcctt
      301 gggaactggc tgaaaaataa gaaaggagtt ctcactgccc tattattttt ataagttctt
      361 atctctttta gcagtgagga ctcctaactg aaagcctaat ggctcttaaa aaagattaag
      421 gcattttatt cttgatttcg gaatatttag ttgcaagaag tcgatttccg gattgctgag
      481 cagcccagat aatatactct acagctattt tattagttgt atcccaatta taccattcct
      541 ctgcatattt aataagttgc tgccaatctt gtttttgcat taagtgagcg gccaatatac
      601 tgtatgtatc ttcattagga cggatatcca atatacgttt aagatattca atactttggt
      661 caagatcatt catctggtct gcaatagaac gagcattttc aagcaagtca agattattag
      721 gttcaatttc taaagcggtt tttgtagcaa tcatcgcctt ttcataatcc ttcaattcat
      781 aataacaagc agatatattc atcaagttat caacattagt aggattttgt actctcgcgt
      841 gatcaaaata tttaactgcc tcagcataat tgcctttatt gtaataaaaa gttcccattt
      901 ctaacaaata ttcaagctta tcaggatctt tctgtagtaa ttgttttaat atttgttctg
      961 ccttaacctc atctttgatg tcaactaagt agatattttt aagttgaatc attggctcgg
     1021 gacgatctgg ttccagttta tgggctaatt caaaaatttc tttagctttt tgtgtgttcc
     1081 ctgcttcttt ttctttttca gcagccatat aaatacgagc ccaagctctt tcttttcttt
     1141 ttctcatatc ccgtaagtca atacggtcct gatttgttag ctcggggaaa ttttcagtta
     1201 cactgatggc ttttgaataa tattcaaagg catcttcata aaattctact tcgcgagcag
     1261 taaaattttc cgctttgaat aaactgatat ctcccaaacg acgcaatgca attacatgat
     1321 cgggattatc ctcaagcact ttcaaatagt aaatttctgc ttgatccaca ttctcttgag
     1381 aatagtaaac atctgcagtt tttaaattca cattaccttg gggactaagc ttttggcgtg
     1441 ctgtagaaca agcagtaagc actaataata gtgcagttaa taagaataac acctttttca
     1501 tccagtattc tcctaagata ttttaatctt aaaaaatcaa agaatatgaa gttgctattc
     1561 agtcaagtaa ttttcgctaa caccttaatt ttttaattcc aagtatcagg ttttactaaa
     1621 tgctaaatta taaagtattc ttgtaaataa gagcactaac gagagaataa aaaccttctt
     1681 aataagtttg tttcctcttg caataactaa tttagaacca acaacatttc ctactatacc
     1741 acaaattgca cctggtatgg caagtggata atatatgtta gcagcaaagg caaaagttat
     1801 cactgctgca aggttagaag ccaagttcac aactttagta ttaccattgc cagttacaaa
     1861 atcgtaatgc agcaaggaag tgtaaataag gagtaaaaat gttccagttc caggaccgaa
     1921 gaaaccatca taaaatccga ttccgagtcc agttattgct gatataatta gtcgatagct
     1981 tttagctaat aattgcgctg tgctttgcat tcccatattt cttttcagta aagaaaaaac
     2041 tgctactgca ggtatcaaaa aaatcaatag ataatttaaa acggcaggcg aaactttcaa
     2101 tgcacttgaa gcacccaacc aggaaccaat taaagcaaat ccagcactta tcacagctac
     2161 agggatatct atcattcttg ctttaaaata attggcagtg gaaaaaagtg ttccgcaaca
     2221 ggaagaaaat ttattggttc ctaaagctaa atgaggtgga attcctacac tccaatatgc
     2281 gggtagagaa attaaacctc ctccacctgc tatagaatca attaaaccag ccaagaagat
     2341 aaagggcaaa acgattaaat aatcaagagg ggttaaaata atattatgca tagtttatat
     2401 ttccgccttg aattccaatt ttagttcctg agtgtctctt tgttttggat aatccgaata
     2461 acgataatca agattaataa cgctgaaact atttatccga taaactccac ttattgatgc
     2521 agaaggaatc caaccagctc ttttttgggg aagaaaagta aaataatcgc ttcctgaaag
     2581 tttattgtag gttactccca atttggcagt taaacggtat ttttgcataa aaacactttt
     2641 catagttggt gataaactta tcgactggag tgtataattt tcactgccat cttgtcttcc
     2701 tccagattct gaactataga taatttctgc ttgaatatta ctttgcagat tcaagttctt
     2761 ttgaatcata gcattaatgc ttaatatttc cgattcattt aaatagcgtg tatcctgatt
     2821 gttttctttg ctaaactgta gacgggtgtt atattcacta taacccttaa tatcaaactg
     2881 tgccaattgt aagaagctat atgttctatc aggtgtttga taacgtttat cgagagtccg
     2941 ttccatagag aattgcaagt tcgatgttat tttattcctt actaaatcta tccagagaac
     3001 ttcctgtatg ttttgacttc catagattgt ggattcgtca ttatacacgc tacccggcaa
     3061 gaagaaataa ctctgccagt tatttcgctt agaattgtgt tcaatcaaat ttactgaagt
     3121 atcactctga agacatttca acaaattgtt cgaggatata tttcctggtt ttagatatat
     3181 attaagcaaa cagtttatct cagaagataa actccctgtg gggcttgtta tataaaccca
     3241 atcataatca ccattgctaa cgcttactcc tgtgctgtc
//
LOCUS       DAORHB010000150         3253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_33452_length_3253_cov_4.136335, whole genome shotgun sequence.
ACCESSION   DAORHB010000150 DAORHB010000000
VERSION     DAORHB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33452_length_3253_cov_4.136335"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(634..1551)
                     /gene="ftsY"
                     /locus_tag="PKM80_04905"
     CDS             complement(634..1551)
                     /gene="ftsY"
                     /locus_tag="PKM80_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNV93284.1"
                     /translation="MKSLKEKLSKTNSNLMGKIAETVKKKKVIDEETLAEIEEILLYS
                     DTGIEMTEIILQRFREQLKKDKVTDPEIAQIYLTDVMRDILLSEDEEIPDFFAEPSVK
                     PYVIVFVGVNGTGKTTTIGKVAYKFSQMGKKVLIVAGDTFRAAAIEQIAIWAERAGVA
                     IVRSQPDSDPAAIIYDGVNAAMARGYDIVLIDTAGRQHTKDNLMKELSKIDRTIKKLI
                     PEAPHEVILVVDATTGQNAISQAHNFDKSIHLTGIALTKYDGTSKGGIIFNLKHNLKL
                     PVKLLGVGEGIEDLMPFKAVPFVKAFFEE"
     gene            1705..2322
                     /locus_tag="PKM80_04910"
     CDS             1705..2322
                     /locus_tag="PKM80_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93285.1"
                     /translation="MESTKIMPRHLLLLFFPIATAILGGLLFSGSFRDFTNNWGGRGI
                     SQAELYLAVSFFIGAIAFSCYCLPKYALWGLLIVIPLLDGILMIVRLGSQFSLILLFI
                     LNLICGILLWLILRYTFLAKSLIKIRTLIFSFVSALVLGTYLKILMLLLKQPKAANTF
                     MDYFLNALVLFIFIGVGISLANLVIVRKEIDEAKKAIREDAEDDD"
     gene            2315..2947
                     /gene="trmB"
                     /locus_tag="PKM80_04915"
     CDS             2315..2947
                     /gene="trmB"
                     /locus_tag="PKM80_04915"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNV93286.1"
                     /translation="MINHKDFFVIEPKMNTEGHYVRLDPQEIFHNSNPLLLEIGSGKG
                     EFISLFSVLHPEFNFIGLESAEKRINNTLKKLSPEQNPNVRLIRLHIDANISQLFPAE
                     SVSGVFIQYPDPWPKRKHHRRRLIQQDFLSALSIILKPSSRVQITTDNRDYAAWILDE
                     FLTHPSFISVYDEPVLKHSPFEEHISTFYEIEQKRQGFEPQYMLFQRIQE"
     gene            2953..>3253
                     /locus_tag="PKM80_04920"
     CDS             2953..>3253
                     /locus_tag="PKM80_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HNV93287.1"
                     /translation="MKYDFSTNYEINKMLNSVVDNITNLAEEQLLHIKRLTRIGESLS
                     SESDLDKIFDMILEEGISFTKADAATIYKVNDEATKLEFEIVYNATLNLLKGGTKG"
BASE COUNT         1008 a          565 c          546 g         1134 t
ORIGIN      
        1 aatctctcgt ttaacaggcc ttaatcaagc gttaataatt aacgcttgat taagggttga
       61 taaacacttg attaacgctt tcccttaaga gcgaagagca aagcttgaat acaatatgta
      121 gcttatccga aattagtaaa cacctgcggg taggtaaata cattaggata gtactcaata
      181 tcaacccaca attttactat atagaggaaa atgagattac tagttcttgc ttggcaaata
      241 aactgaagct caccgacggg ctttatgctt acctgttttt agaagatttt aattcagcga
      301 ttcagttgct gaaaatgtcc ataaactatg tggatttctt attcgggttt ttttactgag
      361 gatgaattcc taataaactg tagaaaagat atattacttc ttccttaagt aactaaactt
      421 actaaaaaag gaggtacttt tttcctttaa tccccaaaac aatattgagg aagtattgaa
      481 gatttaatga atgttattaa ttatggagat ataatacctt gttattctat tcccaaataa
      541 aaaagacctg tagttaatcc tcagtttatc tgtatttctg ccttatataa atagaaacaa
      601 catattatat ccataggaaa tatctgcctg gtatcattct tcaaagaaag cttttacaaa
      661 tggtacagct ttgaagggca ttaaatcttc aattccttca ccaacgccta atagttttac
      721 tggaagtttc aagttatgtt tcaagttaaa aattattccg cctttggatg tgccatcata
      781 ttttgttaaa gcaatacccg ttaaatgtat agacttatca aaattatggg cttgagagat
      841 agcattttgt ccagttgtgg catctaccac taaaatcact tcatgaggag cttctggaat
      901 caatttttta atggtgcgat ctattttact aagctctttc attaaattgt ctttagtatg
      961 ttgtcgtcct gcagtatcaa ttaaaactat atcatagcca cgagccatag ctgcatttac
     1021 cccatcatat ataattgctg caggatcact atcaggttga gaacggacta tagcaacacc
     1081 tgctcgttca gcccaaatag cgatttgttc aatggcagca gcccgaaaag tatctccagc
     1141 aactattaaa acctttttgc ccatttgcga aaatttatag gcaactttcc cgatagtagt
     1201 agtttttcca gttccgttaa caccaacaaa aacaatcaca taaggtttca cagacggttc
     1261 tgcaaaaaaa tcgggaatct cttcatcttc gcttagcaaa atatcccgca taacatcagt
     1321 aagatatatc tgagctattt caggatcggt aactttatcc tttttcaatt gttctctaaa
     1381 gcgttgcagg ataatttcgg tcatttctat tccagtatct gaatatagca ggatttcttc
     1441 tatttcagcg agtgtttcct catctataac tttctttttt ttaactgttt cagcaatttt
     1501 acccatcagg ttgctattgg ttttagatag tttctctttc aggcttttca ctatatttag
     1561 cacttcttta tctccatatc aaattattat tattgacatt ctcttcagtt gcgttaaatt
     1621 tgtattatag gtctataagt caacataaat ttacatacag ttgaaatatg gatttatata
     1681 acttaataaa agtgaaggat ttgaatggaa agcactaaaa taatgccccg gcatttgttg
     1741 ttgttgtttt tcccaatagc aacagcaatt ttagggggtt tgcttttttc cggtagtttt
     1801 agggatttta cgaataattg gggaggcaga ggcatcagtc aagcggagct atatttagct
     1861 gttagttttt ttattggtgc tattgctttt agttgttatt gtttacctaa atatgcgctt
     1921 tggggattgc ttattgttat accgttatta gacgggattc ttatgatagt tcgattgggc
     1981 tcgcaattta gccttatctt acttttcatt cttaatctga tttgtggtat tttactttgg
     2041 ttgatattgc gctatacttt tttggcaaag tctctcatca agatacggac tttgatcttt
     2101 tcatttgttt cagctttggt cctaggaaca tatttgaaga tattaatgtt acttttaaaa
     2161 caacctaaag ccgctaacac ctttatggat tattttttaa atgccttggt attatttatt
     2221 ttcattggtg taggcatttc tttagcaaat ttagttatcg ttcgcaaaga aattgatgaa
     2281 gcgaaaaaag caattagaga ggatgcagaa gacgatgatt aatcacaaag atttcttcgt
     2341 aattgaaccc aaaatgaata ctgagggtca ttatgtgcgt ttagatccac aagaaatatt
     2401 tcataattcc aaccctcttt tactggaaat tggttccggt aagggagaat ttatatccct
     2461 tttttccgtg ctacatccag aattcaattt tatcggattg gaatcagcag aaaagaggat
     2521 aaataatact ttaaaaaaac taagccctga gcagaaccct aatgttcgtc ttatccgact
     2581 tcatattgat gctaatataa gccagctttt tcctgccgaa tctgtttcag gtgtttttat
     2641 tcaatatcca gatccctggc cgaaacgaaa acatcatcgc agacgcctta tacagcaaga
     2701 ttttttaagt gctttatcta taattctaaa gccctcatca cgggttcaga ttacaactga
     2761 taatcgagat tatgctgcct ggattttaga tgaattttta acgcatcctt cctttatctc
     2821 agtttatgat gaaccagttt taaaacattc accttttgaa gagcatatta gcacttttta
     2881 cgaaatcgaa caaaagcgtc aaggttttga accgcaatat atgctttttc agcgtattca
     2941 ggagtaaaca aaatgaaata cgattttagc acaaactatg agattaacaa aatgctaaac
     3001 agcgtggttg ataatatcac taatctcgca gaagaacaat tgttgcatat caagcgtctg
     3061 acccgtattg gcgaatcact ttcttctgaa agtgacttag ataaaatttt tgatatgatt
     3121 ctggaggaag gtatttcttt tacaaaagct gatgcagcaa caatctataa agtaaacgat
     3181 gaagccacga aactagaatt tgaaatagtt tataacgcaa ctttgaattt acttaaaggt
     3241 ggtacaaagg gac
//
LOCUS       DAORHB010000151         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_33543_length_3248_cov_3.247416, whole genome shotgun sequence.
ACCESSION   DAORHB010000151 DAORHB010000000
VERSION     DAORHB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33543_length_3248_cov_3.247416"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..742)
                     /locus_tag="PKM80_04925"
     CDS             complement(107..742)
                     /locus_tag="PKM80_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNV93288.1"
                     /translation="MTTHNQDWKDIANAQFSKALALSLTLLIFGIMVTPKVEVRKQVF
                     QTQQMELVDIPMEERERIEAPQTEVDVNVSFQISDVLGTEEVDMAAFQEALSQIGDIY
                     KTTTTSVQQQEDERVVNFVPYDDAPVIIGKIEPVYPEFAKRNKLQGTVVLEVEVLKDG
                     SIRDIRVRRGVGGGLDEAAIEAVRKVRFQPGKSSGQAVDCLVIIPVEFKIQ"
     gene            complement(755..1189)
                     /locus_tag="PKM80_04930"
     CDS             complement(755..1189)
                     /locus_tag="PKM80_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNV93289.1"
                     /translation="MAKFSTKKRRDVAIPQASTSDIAFLLLLFFMVTTVFVQEKKFWV
                     ERDRWPHAESIERIPRNHTSTIYVMRDETILLDDIPTRIESSEDVFISQTLIRKKAED
                     PELIVCFRTDRDTKYGVMSDIMRQLQDANTLVVAFETQPLLK"
     gene            complement(1199..1597)
                     /locus_tag="PKM80_04935"
     CDS             complement(1199..1597)
                     /locus_tag="PKM80_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNV93290.1"
                     /translation="MAAIPTASTGDIAFLLIIFFMSTTKFDVKEGIKLVLPQAAEVES
                     GKTQTLTLTEKEMTRIQILPDGQIALNREAPRYIESSELDVIIQQKVQINPDMIFKVI
                     TDRETKYSEMIRVVDRLKAAKIEKISLSTS"
     gene            complement(1621..2385)
                     /locus_tag="PKM80_04940"
     CDS             complement(1621..2385)
                     /locus_tag="PKM80_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNV93291.1"
                     /translation="MRKFIGTILLIMMMTFVFASFAYAQENTAAADQGGGGLQNIAEL
                     VFGSGLVKWFKDGGWAMWPILFLAIYGLAYIIWKFIALLTGKVNLNAFLNKIVPLIKD
                     KKYEEAKEIASKTRGPVASIIYAGLEKIDKGIDAVDEAIENASMIEMSYLEKGFIELS
                     NSITLAPMLGFLGTVAGMITAFDSIAAARAVDATIVATGIKIALITTEAGLIVAIPVQ
                     FFNNVFMTMVDGIVIDMQRASEKVTEILAESIGTKK"
     gene            complement(2412..>3248)
                     /locus_tag="PKM80_04945"
     CDS             complement(2412..>3248)
                     /locus_tag="PKM80_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93292.1"
                     /translation="QVGNPSLEPQITITYEVGLSHQLSEDYVLDLTAYYKNLYNYVNT
                     RKERSEIEQTVYWYRYYSNDYGSARGIDMQIEKLLSNFNTWSLAYSLAWAQGNSSYTV
                     IQDETTNLREFPLDWDIRHNISANFTFHIARGEEYFIPFTDYILPLDDFSANFNWTFA
                     SGAPYTPQSAESNSSLDTNSKRKDNTQQANLRLTKGFVLPNNMSIRIYAEIENLFKNM
                     NVLNVYPKTGNYYWGGDNLEEANIPGFVYDEVQYVYSLAYKNPTYYNNFRGLTVGVSF
                     NF"
BASE COUNT          976 a          686 c          534 g         1052 t
ORIGIN      
        1 ccagttgagg gaactgcctt cgctccatat actgagctta tagaaaatat aaggagtagg
       61 caaaggcaaa ttccaatttt atacaacttc attaagcact cccaatttat tgaattttaa
      121 attctaccgg aattataacc aaacagtcaa ctgcttggcc actacttttc ccgggttgaa
      181 atcttacttt acgcacagct tcaatagcag cttcatccaa accgcctcct accccgcggc
      241 gaactcttat atcacgaatg ctgccatctt ttaaaacttc cacttccaat actacggttc
      301 cctgcaattt gtttcgttta gcaaattcag gataaacagg ttcaatttta ccgataatta
      361 ctggagcatc atcataggga acgaaattta ccactctctc atcttcttgt tgttgaacac
      421 ttgtagttgt tgttttgtag atatctccaa tctgcgaaag tgcttcctga aaagcagcca
      481 tatccacttc ttcagttcct aatacatcgc tgatttggaa agatacatta acatcaactt
      541 ctgtttgcgg tgcttctatc cgttctcgtt cttccatcgg aatatcgacc aattccattt
      601 gttgggtttg aaaaacctgt ttacgaactt caactttcgg agttaccatt atgccaaaga
      661 taagcaaggt aagagataaa gccagtgctt tgctaaattg tgcattagca atgtctttcc
      721 aatcttgatt gtgtgttgtc atcttatcca ccttttattt tagcaatggt tgagtttcaa
      781 aggcaactac caatgtgtta gcatcttgaa gttgccgcat tatatcagac attactccat
      841 atttcgtgtc tctatctgtt cgaaaacaaa ctatcagctc gggatcttcc gctttttttc
      901 ggataagggt ttgtgaaatg aatacatcct cagagctctc tatccgggta ggaatatcat
      961 ccagtaaaat agtttcatct cgcataacat agattgtact tgtatgattg cgcggaatgc
     1021 gttcaatact ttctgcgtga ggccaacgat cgcgttctac ccagaacttt ttttcctgca
     1081 caaaaactgt ggtcaccata aagaacagca acaagaggaa ggcaatatca gaggtagatg
     1141 cctgaggaat ggcaacatca cgtctttttt ttgtactaaa tttagccatt tctttctcct
     1201 aacttgtaga tagagagatt ttttcaatct tagcagcttt caagcggtca accactctaa
     1261 tcatttcgct gtattttgtt tcacggtcgg ttatgacctt aaaaatcata tctggattaa
     1321 tttgtacttt ctgctgaatg ataacatcca gttcgctact ttcaatatat cgcggtgctt
     1381 ccctgtttaa cgctatttgc ccatcaggta ggatttgaat acgagtcatt tccttctcag
     1441 taagcgtaag agtttgagtt tttccacttt ccacttcagc cgcctgtgga agaacaagct
     1501 taataccttc tttcacatca aacttggtag tggacataaa gaagatgatc aacaaaaagg
     1561 caatatcacc agtggaagct gttgggatag ctgccatgta tttcctcttc cgagtaattt
     1621 tcatttttta gtacctatgc tttcggctag tatttctgta accttttcgg atgctctttg
     1681 catatctata actattccat ctaccatagt cataaacaca ttattaaaga actgaacagg
     1741 aatagctaca atcaatcctg cttcagtagt aataagtgca attttaatac ctgtggccac
     1801 aattgtagca tcgacagccc gagcagcagc aatagaatcg aatgcagtga tcattccagc
     1861 aacggtacca aggaatccta acataggagc aagtgttata gaattagata gctcaatgaa
     1921 tcccttttcc agatagctca tttcaatcat agaagcattt tcgatagctt catccacagc
     1981 atcaattcct ttatctattt tttctaatcc tgcataaata attgatgcta caggtcccct
     2041 tgttttactg gcaatttctt tcgcttcctc atacttttta tcttttatta aaggaacgat
     2101 cttgttgaga aaagcgttta aattaacttt accagtaagt aaggcaataa acttccagat
     2161 aatataagct aagccataga tagccaagaa gagtattggc cacatagccc atccaccatc
     2221 cttgaaccat ttaaccagac cactgccaaa tacgagctct gcaatatttt gtaacccgcc
     2281 accaccttga tcagctgcag cagtattttc ctgtgcataa gcgaaactcg cgaatacaaa
     2341 ggtcatcatc attatcaaca gtattgttcc gatgaatttt ctcattttta ttaattcctc
     2401 cttggaataa tttagaagtt aaacgagacg cccacagtaa gccctcggaa attgttataa
     2461 taagtgggat ttttgtatgc gaggctataa acatattgta cttcatcata aacaaatcca
     2521 ggaatattag cttcttccag gttgtccccg ccccaataat aattgcctgt tttaggataa
     2581 acatttagaa cattcatatt tttaaacaga ttttctattt ctgcataaat tctgatactc
     2641 atattattag gcaaaacaaa acccttagtt aatctcaaat tcgcttgctg agtattatct
     2701 tttcttttgc tgttagtatc taatgaacta ttagattctg cactttgagg agtgtatgga
     2761 gcaccagaag caaaagtcca attaaaattc gcagaaaaat catcaagagg taaaatataa
     2821 tcagtaaacg gaatgaagta ttcctcgcct cttgcaatat ggaaagtgaa attagcactt
     2881 atattatgcc gtatatccca atccaaagga aactctctca aattagttgt ctcatcttgg
     2941 ataactgtat aggagctatt accctgagcc caagctaatg aataagcaag gctccaagtg
     3001 ttaaagttag agaggagttt ctctatctgc atatctatac cacgagcaga gccataatca
     3061 ttagaataat atctgtacca ataaactgtt tgctcaattt cgctacgctc ttttcttgta
     3121 ttcacatagt tatataaatt tttataatac gcagttaggt ctaaaacata atcttcacta
     3181 agttgatgag aaagcccaac ttcgtaagta atagtaatct gaggttccaa actaggattt
     3241 cctacttg
//
LOCUS       DAORHB010000152         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_34209_length_3205_cov_5.476190, whole genome shotgun sequence.
ACCESSION   DAORHB010000152 DAORHB010000000
VERSION     DAORHB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34209_length_3205_cov_5.476190"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /gene="argS"
                     /locus_tag="PKM80_04950"
     CDS             complement(<1..1285)
                     /gene="argS"
                     /locus_tag="PKM80_04950"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNV93293.1"
                     /translation="MIKQLLSNNIIEALDTLKLSHEKEFTVEIPNNPEFGDYSTNAAL
                     VLGKENKQNPMQLAEKIVKELKKNKAYKKVEIAKPGFINFHLAPSLYQQVFWEIYKNQ
                     EYGSSDFGNGEKVLLEFVSANPTGPLNIVSARAAAFGDTIYRIMKYVGYNPAREFYIN
                     DAGNQVDILAESLELRLREIHGEKIGEFPYEAYHGEYVKHLAHRLNALEGTRVFMMPE
                     KDRLEHLKEFALSELLEMQRNSLEKFGVVFEGWVSEKTLRSEGVVEEVLSYLTEADCT
                     YEKEDAIWFCSTKYGDDKDRVLMKSDGSITYFVPDVAYHLTKIQRGFTKLIDIFGPDH
                     HGYVPRIRAAFRALNYDDSILEFIFLQQVNIFESGERVKMSKRAGKIVTMDELISVVG
                     KDAARYFFIARKANAHLNFDLELALKKNNENPVYYC"
     gene            complement(1297..1632)
                     /gene="rsfS"
                     /locus_tag="PKM80_04955"
     CDS             complement(1297..1632)
                     /gene="rsfS"
                     /locus_tag="PKM80_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNV93294.1"
                     /translation="MENNKLTAVISWLEEKKAENIRIYDVEEKSDYTDTIVVCEGTAD
                     IHNKAIASYLMEMAKKNHLQLLSKEGLEYGHWVLIDIGELIVHIFLPETREYYHIDDL
                     LTKFVNKTS"
     gene            complement(1632..2096)
                     /locus_tag="PKM80_04960"
     CDS             complement(1632..2096)
                     /locus_tag="PKM80_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNV93295.1"
                     /translation="MNLTNNPPENNLPPRRKIWFPIILILVATAAVVLFFGLRNKAIE
                     PKTGYEEDQLPSIKIVVTNGCGYEHLAADFASALKDKNIEVVSLSQTPKPIYDKTIIV
                     IRKGDMQDLERLKKMTGIQRWTSAYNEYYSADFEIIVGRDYEQFIPKKGRRD"
     gene            complement(2093..2629)
                     /locus_tag="PKM80_04965"
     CDS             complement(2093..2629)
                     /locus_tag="PKM80_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNV93296.1"
                     /translation="MNNQNDNHMDKHYLYSPWRLDYILGEKTDECIFCRCQNLDNDKE
                     NLIVYRAKNCYVMLNRYPYNNGHLMIIPYQHSASITDLDDKIWLEMCSLLKDTEQILT
                     RVYNCDGINIGINLGCAAGAGIAEHIHIHIVPRWNGDSNFMSVIGGERVIPESFENAY
                     NKLAPEFANLIKNKEING"
     gene            complement(2622..>3205)
                     /locus_tag="PKM80_04970"
     CDS             complement(2622..>3205)
                     /locus_tag="PKM80_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional N-acetylglucosamine-1-phosphate
                     uridyltransferase/glucosamine-1-phosphate
                     acetyltransferase"
                     /protein_id="HNV93297.1"
                     /translation="GEICGIKEFKDATDEQRKIKEWNTGIYCFKAKDLFTSLKQTSNN
                     NHQGEYYLTDTVEILSRAGKKISNVVLEDLMEVSGVNSQEELAALEDIYIGRIRKKWL
                     NNGVIVHNPASVYIGDDVIIEPDVEIYQNTVIKGKCTLEKGCIIGPCCLLENSDVSTE
                     AIMQGYNVLVNAVLPEHHILHYGSHIIEETQYE"
BASE COUNT          947 a          649 c          512 g         1097 t
ORIGIN      
        1 gacaataata cacagggttt tcgttatttt tctttagcgc taattccaaa tcaaaattaa
       61 gatgagcatt tgccttacga gcaatgaaaa aatagcgagc ggcatcttta cctacaacac
      121 ttatgagctc atccatagta acgatttttc ctgcgcgttt actcattttt actctttcgc
      181 cgctttcgaa aatattaacc tgctgcagga aaataaattc caagatagaa tcgtcataat
      241 tcaaagcacg aaaggcagct ctgattctgg gaacataacc atgatgatca gggccgaaaa
      301 tatcaatcaa ttttgtaaaa ccgcgttgga ttttagttaa atgataagca acatctggaa
      361 caaaatatgt gatggatcca tcagatttca tcaatactct atctttatca tcgccatatt
      421 ttgtgctgca aaaccagata gcatcttctt tttcgtaagt acaatctgct tcagttagat
      481 aggaaagaac ttcttcaact actccttctg agcgtaaagt tttttccgaa acccagcctt
      541 caaaaaccac accaaatttt tccaaagaat ttcgttgcat ttctaaaagc tcggagaggg
      601 caaattcttt caagtgttcc agacggtctt tttctggcat cataaaaact cgggtgcctt
      661 ctaaagcatt caaacggtgt gcgagatgtt ttacatactc gccatgatat gcttcgtaag
      721 ggaattcacc tattttttct ccgtgaattt ctcgtaaacg caattcgagc gattctgcta
      781 aaatatcaac ttggttacct gcgtcgttaa tataaaactc tctggcaggg ttataaccta
      841 catatttcat aatcctataa atagtatcac caaaagctgc ggcacgggca ctaacaatat
      901 ttaaggggcc tgttggattg gcacttacaa attccaaaag tactttttct ccgttgccaa
      961 aatcgcttga tccgtattct tgatttttgt atatctccca aaaaacctgc tgatataaag
     1021 aaggtgcaag atgaaagtta ataaaaccgg gcttggcaat ttccaccttt ttgtatgcct
     1081 tattcttttt cagttcttta actatttttt ccgccagctg cattggattt tgcttatttt
     1141 ctttgcctaa aactaaagcg gcattggtag aataatcgcc aaattcaggg ttatttggaa
     1201 tttccacagt aaattccttc tcatggctta gttttagggt atctaaagcc tcaatgatgt
     1261 tattactaag aagttgtttt atcatttatt actctcttac gatgttttat ttacaaattt
     1321 ggtaagaaga tcgtctatgt gataatactc tcttgtttca ggtaaaaaaa tatgcacaat
     1381 caattctcca atatcaatca agacccaatg tccatattcc agaccttctt tactaagcag
     1441 ctgcagatga tttttcttag ccatctccat aaggtaactg gctatagctt tattatgaat
     1501 atcagcagtg ccttcgcaga cgactattgt atctgtataa tcgctttttt cttcaacatc
     1561 atagatgcgt atattttctg cttttttttc ttccagccag gaaataacgg ctgttaattt
     1621 attgttttcc actaatctct tcttcctttt ttgggtataa attgctcata atctctgccg
     1681 acgataattt caaaatcagc actataatat tcattgtaag cacttgtcca tcgctggatt
     1741 ccggtcattt ttttcaatcg ttccaaatcc tgcatatctc ctttacgaat aacgataatg
     1801 gttttatcat aaatcggctt cggagtttgt gacaaactga ctacttcaat attcttatcc
     1861 ttcagtgctg aagcaaaatc cgcagctaaa tgttcataac cacaaccatt agtaactact
     1921 atcttaattg atggaagctg gtcttcttcg tatccagttt tgggctcaat agctttattt
     1981 cttaatccaa agaagagcac aactgctgcc gtagcaacaa gaataaggat aatcggaaac
     2041 caaattttcc ttcttggggg caagttattt tcaggcgggt tatttgtcaa attcaaccgt
     2101 taatctcttt attttttata aggttggcaa attcaggagc cagtttatta taggcatttt
     2161 cgaaagattc cggaattact ctttcaccac caatcacgct cataaaatta ctatcaccat
     2221 tccaacgcgg aacaatatga atatgaatat gttctgcaat tccagcacca gcagcacagc
     2281 caagattaat accaatattt attccgtcgc agttatacac gcgagttaag atctgttctg
     2341 tatcttttaa taaagaacac atttccagcc agattttatc atccaaatcg gttatagatg
     2401 cagaatgctg ataggggata atcattaggt gaccattgtt atagggatag cgatttagca
     2461 ttacatagca atttttagct ctatacacaa tcaggttttc tttgtcatta tcaagatttt
     2521 ggcaacggca aaaaatacac tcatccgttt tctcccctaa aatataatcc aatcgccagg
     2581 gtgaataaag gtagtgttta tccatatgat tatcgttttg attattcata ctgtgtctct
     2641 tcaattatat gagagccata atgtaaaatg tggtgttcag gtaaaacagc gttaaccaat
     2701 acattataac cttgcataat ggcttctgta gaaacatcac tattttccag taagcaacag
     2761 ggaccaataa tgcatccctt ttccaaagta cattttcctt tgataactgt gttctgatag
     2821 atttctacat caggttcgat tatcacatca tcacctatat agacgctggc aggattgtga
     2881 acgataactc cgttatttag ccatttttta cggatacggc ctatatagat atcttccaaa
     2941 gcggccaatt cttcctgaga atttactcct gaaacttcca tcagatcttc caaaactaca
     3001 ttggagattt tttttcctgc tctggataag atttcaactg tgtcggtgag ataatattct
     3061 ccttgatgat tattattaga ggtctgcttt aaggaagtaa ataggtcttt tgccttaaaa
     3121 cagtaaatgc cggtattcca ttccttaatt tttctttgtt catcggtggc atctttaaat
     3181 tcttttattc cacaaatttc gcctt
//
LOCUS       DAORHB010000153         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_35872_length_3106_cov_4.038348, whole genome shotgun sequence.
ACCESSION   DAORHB010000153 DAORHB010000000
VERSION     DAORHB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35872_length_3106_cov_4.038348"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(538..1491)
                     /locus_tag="PKM80_04975"
     CDS             complement(538..1491)
                     /locus_tag="PKM80_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNV93298.1"
                     /translation="MTSITKKQYLRRETGRLIGIIFASVFFAMGYSWFLLPYNMAPGG
                     VGGLSQILYYFFGIPNGVSMILINIPLFIVSFIFIGKSFGSKSLYGMFVSAIMTDFLS
                     FSSLHKIGIIKDLTPYTHILDGRKIYAMLPPEDMLLSAIAGSVLLGIGLGLIFRFRGS
                     TGGTDIPVALIKQKANLSIGTGYYIVETGIILLVAIVLKSPKLLIWGYLNLFITTKIT
                     DLVSEGLPYIKGAYIISEAVDEISKEVFEKLERGATFINGVSGYHQRDIKILFVVLNR
                     RQVPQLTDIVKDNDPDAFMIIMDVYDVLGYGFKSRSINLSE"
     gene            1603..>3106
                     /locus_tag="PKM80_04980"
     CDS             1603..>3106
                     /locus_tag="PKM80_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNV93299.1"
                     /translation="MILDIRLNGIKHSLIEVDKPLPLTEILKRTHIDRSQILSYKINH
                     REYVNEDYIPDRETLIDCITYLHPEGYRIYQDSTIFIMCKALHTLMGTEPSLVVEHSI
                     ADGVFCEVFNSQTFTEEDCSRLIAEMQNIVENNLPIEKITVKTDEALDIFSALGRNDV
                     IKNIKYHYQENVELYRCGKYYDCYNHPLTDRTGKIKNFDIVYHSPGFILRFPSKDENM
                     EIPTFQLPYKLFARHQEHDKWLDILRVHNIIDINKLIDHYEISEFIQVEEALHEKKIA
                     EIAADIVTQKEVKLILIAGPSSSGKTTFAKRLGVQLQACKAKPVVIGMDDYFLPRTKT
                     PRKPNGEYDFETIYAMDLEYLNMQMNQLLDGEQIELPRYDFTRGIRRRSNTFVKLGSN
                     SIIVMEGIHGLNEILTASIPANRKLKIYVSALNQLNIDNHNRISTTDCRLLRRIIRDH
                     RYRGYSAEETLMRWQDVRDGEDKYIFPYQENADYMFNSSLTYEIGVIRKYA"
BASE COUNT         1050 a          591 c          538 g          927 t
ORIGIN      
        1 agaaacaagg atttaaggat tttaggatta agaggattta tttataagcc accgaggcca
       61 ccgaaaaaaa tatcctctca ctgattacac ggattacaca gatttgcatt ttaagaacaa
      121 ggattttaga ataagttcac gcagattacg ccgattatgc agatttgcat tttaagaaca
      181 aggattttag aataagttca cgaagattac acggattaca gatatataag caactttagg
      241 ttcaaaggca tcgctatcct gaatatgcat cctgttaagt agactttttg cgcatatcct
      301 tctccttaaa ttcagtccac tattttcaag atgattcaaa caaacaaccc atcctcctct
      361 catcttcctc tcctcctcct cccgtcctca aaccgtcgtt ggggaggagt gaaggttagg
      421 tagatgacga gttatatctg agtggtctac aaggaaatta tgtatagacc taaattaata
      481 gccacccatc ctattctcat taaacaatca taagtggagg atgggtgatg atcaggatta
      541 ctcgcttaaa tttatgctgc gagacttaaa tccatatccc agaacatcat acacatccat
      601 aataatcata aaggcatcgg gatcattatc tttaacgata tcagttaatt gtggaacctg
      661 gcgtctgttt agcactacaa aaagtatctt tatatctctt tgatgataac cactaactcc
      721 atttataaat gttgcccccc gctcgagttt ttcaaatact tctttactaa tttcatctac
      781 tgcttcggag ataatataag ctcccttaat ataaggaagt ccctcactaa ctaaatcggt
      841 tatttttgta gtaataaaca gatttaaata gccccagata agcagtttag gtgatttaag
      901 cacaatggct accagaagaa taattccagt ttctacaatg taataacctg tcccaattga
      961 aagatttgcc ttttgtttaa taagtgctac aggaatatcg gttcctcctg tagaacctct
     1021 aaaccgaaaa ataagtccca atccaatccc aagtagcaca ctacctgcaa ttgcgcttaa
     1081 aagcatatct tctggaggaa gcatagcata aattttacgc ccatctaaaa tatgtgtgta
     1141 gggtgttaaa tctttaatta tacctatttt atgaagagat gaaaagctta ggaagtcggt
     1201 cataatagcg ctcacaaaca ttccatataa acttttactg ccaaaggact tacctataaa
     1261 gataaagctt actataaaca gtggtatgtt tattagaatc atagataccc cattgggtat
     1321 gccaaaaaag taatacaaaa tttgagataa cccccccact cctccgggag ccatattata
     1381 tggtaataaa aaccaggaat aacccatagc aaagaaaacg gaggcaaaga taattcctat
     1441 caggcgtcct gtttctctac gcagatactg ttttttcgtt atacttgtca tttgctctct
     1501 ctcctctata tcacttgact aattttttgt ttttaaatac ttggtatgat aaaaatgact
     1561 attataattc cccattagca atgggaataa cagggaaaaa agatgatatt agacattcgc
     1621 ttaaacggaa tcaaacattc acttatcgag gtggataaac cccttccact tacagagata
     1681 ctaaaaagaa ctcatataga cagatcacaa atattgtcct acaaaataaa ccatcgggaa
     1741 tatgtcaatg aagattacat ccccgatagg gaaacactaa tagattgtat cacttatctt
     1801 catcctgaag gttatcgaat ttatcaggac agcacaattt ttattatgtg caaggcattg
     1861 catacattga tgggaacgga accatcttta gtagtagaac attctattgc agacggtgtt
     1921 ttttgcgaag tttttaattc gcagactttt accgaagagg attgcagtcg cctaatagca
     1981 gaaatgcaaa acattgtgga aaataatcta cccatagaaa agataacagt taagacagat
     2041 gaagcattgg atatttttag tgctttaggc cgcaatgatg ttatcaaaaa tatcaaatac
     2101 cactatcagg aaaatgtgga actttatcgc tgcgggaaat attatgactg ctataatcat
     2161 ccgctaaccg atagaaccgg aaaaatcaag aactttgata ttgtatatca ttctcccggt
     2221 tttattttac gctttccctc caaagatgaa aatatggaaa ttcctacttt tcagcttccg
     2281 tataaattat ttgccaggca tcaggaacac gataaatggc tggatatatt acgcgttcat
     2341 aatataatag acatcaataa attgatagac cactatgaaa tctcggaatt tattcaagtg
     2401 gaagaagctc tacacgagaa aaaaatagca gaaatcgctg ccgatattgt tacccaaaaa
     2461 gaagtgaaac taatccttat tgccggtccc tcttcttcgg gtaaaaccac ctttgccaaa
     2521 aggttgggag ttcagcttca agcctgtaaa gctaaacctg tagttattgg aatggatgat
     2581 tattttctgc cgagaacaaa aactccccgc aaacccaatg gtgaatatga ctttgaaacc
     2641 atatatgcta tggatttgga atatctgaat atgcagatga atcaattgct ggatggcgaa
     2701 cagattgaat tgccccgcta tgattttacg cgagggattc gtaggcgcag caatactttt
     2761 gtaaaacttg gttctaacag cataattgtg atggaaggta ttcatggttt aaacgaaatt
     2821 cttactgctt ccattcctgc caaccgtaaa ttgaaaatat atgttagtgc gctcaatcaa
     2881 ttgaatatcg ataatcataa tcgaatatct acaactgatt gccgattgtt aagaagaata
     2941 attcgcgatc atcgttatag aggttatagt gcagaagaaa ccttaatgcg ctggcaggat
     3001 gtacgcgatg gtgaagataa atacattttc ccttaccagg aaaatgctga ctatatgttt
     3061 aacagcagtt taacttatga aattggcgta attcgtaaat atgcct
//
LOCUS       DAORHB010000154         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_36851_length_3056_cov_4.817061, whole genome shotgun sequence.
ACCESSION   DAORHB010000154 DAORHB010000000
VERSION     DAORHB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36851_length_3056_cov_4.817061"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..1308
                     /locus_tag="PKM80_04985"
     CDS             250..1308
                     /locus_tag="PKM80_04985"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNV93300.1"
                     /translation="MAWLFVYGLTPFIIRLASALNFVDKPEARKIHLKSVPLLGGLSV
                     ATGFVLLCIYDVLISPGRFLDEPMLGYLAGSVLIVIIGLIDDHRGMKPVLKMFGQLVV
                     SLLFIFTNFTLAELSLMFGSIYISLPIMLLWMVGLMNALNFLDNMDGIISGMAGILGL
                     GFFAFALINTTQANQEAMALMALIALSFAGSAFGFLPYNFNPAHIFLGDAGSMFIGYF
                     LSSMGILMARYAANRYNDKFFYLLPVLLLSYAIFDISLVSYTRRRDGRQVMQGGKDHS
                     THRMNTILGSIKITAIMIYTINVLIVLTSIIIFRIGNRQLLIITTAIFAAFFIFFGRK
                     LDKVPIVVPANQQKHKTE"
     gene            1311..2048
                     /locus_tag="PKM80_04990"
     CDS             1311..2048
                     /locus_tag="PKM80_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV93301.1"
                     /translation="MEVTILGSGSGLPQLDKHLSSILIRQANKLFLADCGDSCSHRLL
                     EYGIDADQLDAIFVTHYHPDHLGGLFMLLQMLYLQNRSRDLLLFLPERLASFLEIMQL
                     MYTYPQKFGFNLLIRCIEEVKLYYDWISAVPNDHLYRYAPIITEYHLPNQMKSWSLCF
                     NVANGKFVYTSDLETTDCISDTLKGAHTALVDAGHPQAEQILKLEYAGIQRIILTHGI
                     NPELENRKNELNPEIFEFAREDFVYKI"
     gene            2032..>3056
                     /locus_tag="PKM80_04995"
     CDS             2032..>3056
                     /locus_tag="PKM80_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93302.1"
                     /translation="MFTRYRLIFLLLLSSLLLLTGCEINRLKSAEDLYQKHLYATAIQ
                     ELDDLIANAENRAIATKAEILRSNSYIALAEAAVEKKNWPLAIRFFKLSNSDAANFRL
                     AEIYRDLAVEASKAGNLELSKIYLDTIYREVPETPIIPEVLYRRIAYYVDVKADNEAA
                     WADYMQLYDNYPNNPYEVLARNYVIRFIPGKLDYAAILNEQGYYTDALKELFEISKYP
                     VVDMDRLNKQISDVYERQAEELINEQDYVEADRLFRIALQYYPQKKDSITNRLESITN
                     LFIQKGNSLLEAGDYEAALIHYRKTFDIIPDYPPAVEAIKRVYTIQENIRKAGELFSQ
                     AERLEATG"
BASE COUNT          908 a          490 c          608 g         1050 t
ORIGIN      
        1 cttaatcaag cgttaataat taacgcttga ttaagggatg attagcgctt gattaacgct
       61 ttccatcaag ggcgaagagc ggagcttgca tctgtataca attaacccta tgctgaagat
      121 agttattttt ttatttattt cattcttcat tcgttcttga caaaaacgct ttcttttttt
      181 gcttggtaag aaaaagtatg gagataagca atggaaaacc ggatctatga atacttaagt
      241 gtattcataa tggcgtggct atttgtatat ggtttaacgc ctttcataat ccgtttagca
      301 agtgctttaa attttgtaga taagcccgaa gcgcgcaaga tacacttaaa aagtgttcca
      361 cttttgggag gtttaagtgt tgctacaggt tttgtattgc tttgtatcta tgatgtatta
      421 atttcaccag ggcgtttttt ggatgaaccg atgctgggtt atcttgctgg ttcagtatta
      481 attgtgataa tagggctgat tgatgatcat cgaggaatga agccggtttt aaaaatgttt
      541 ggtcaattag tggtaagttt attattcatc ttcaccaatt ttacattagc tgagcttagt
      601 ttgatgtttg gcagcatata tatttctctg ccgataatgt tgctttggat ggtaggactg
      661 atgaatgcgc tgaacttttt agataatatg gatggcataa taagtgggat ggctggcatt
      721 ttaggtttgg gtttttttgc ttttgccttg atcaatacca ctcaagcaaa tcaggaagcg
      781 atggctttga tggcattgat tgcacttagt tttgcaggtt cggcttttgg ttttttgccc
      841 tataatttca atcccgctca tattttttta ggtgatgcgg gcagtatgtt tattggttat
      901 tttctttctt cgatgggcat tttgatggcg cgttatgctg cgaatcgtta taatgacaaa
      961 tttttctatt tattaccagt tctattactt tcctatgcaa tttttgatat ttcgctggta
     1021 agttacactc gcagaaggga tggaaggcaa gttatgcaag gtggaaagga tcacagtacg
     1081 cataggatga atactatttt ggggtcaatt aaaataacgg cgataatgat ttacactatc
     1141 aatgtattaa ttgtcctcac ctccattatt atcttcagga taggtaatcg tcaattactt
     1201 ataattacca cagcgatatt tgctgctttc tttatcttct ttggtcgcaa actggataaa
     1261 gttccaatcg ttgttccagc caatcaacaa aaacataaaa cggagtaaat atggaagtaa
     1321 ctattttagg ctcgggatct ggtctacccc aacttgataa acatctttcc agtattttaa
     1381 ttcgccaggc taataagctt tttttagcag attgtggaga tagctgttcc caccgtttat
     1441 tggaatatgg tatagatgca gatcaattgg atgctatttt tgtaactcat taccatcctg
     1501 atcatttggg tggattattt atgcttttgc agatgttgta cttgcaaaat cggagccgtg
     1561 atcttttact ttttttacct gaacgtcttg cttcctttct ggaaattatg cagctgatgt
     1621 atacttatcc tcagaaattt ggttttaact tgctgatccg ttgtattgaa gaggtaaaat
     1681 tatattatga ttggatatct gctgtgccta atgatcatct atatcgatat gctcctataa
     1741 ttactgaata tcatcttccc aatcagatga aatcctggtc attatgtttt aatgtagcta
     1801 acgggaaatt tgtatatacc agcgatttag agacaactga ttgcatatcg gatactttaa
     1861 agggtgctca tactgcatta gttgatgcgg gtcatcctca agcagaacaa attttaaaat
     1921 tggaatatgc aggaatacaa agaataatcc ttacgcatgg aataaatccc gaactggaaa
     1981 accgcaagaa tgagctaaat cctgaaatat ttgaatttgc aagggaagat tttgtttaca
     2041 agatatagat taattttttt attactactt agttcattgt tacttcttac aggttgtgaa
     2101 attaaccgat tgaagagtgc agaggatctt tatcaaaaac atttatatgc tactgccatt
     2161 caggaactgg atgatcttat tgctaatgca gaaaatagag ctattgccac taaagctgaa
     2221 atattgcgtt cgaacagtta tattgcttta gcagaagcag cagttgaaaa gaaaaattgg
     2281 cctttggcaa tccgattttt taagctatcc aattctgatg cagccaattt tcgcttagcg
     2341 gaaatatatc gagatttagc agtggaagca agtaaagctg gcaacctgga attgagcaag
     2401 atttatttgg ataccatcta tagagaagtt ccagaaaccc ctataattcc tgaagtttta
     2461 tatcgtagaa ttgcctatta tgttgatgtt aaggcagata atgaagctgc ctgggcagat
     2521 tatatgcaat tatatgataa ctaccccaac aatccttatg aggttttagc ccgaaattat
     2581 gtaatacgct ttattcctgg aaaattagat tatgctgcaa ttttaaatga acagggttat
     2641 tataccgatg ccttaaaaga gctttttgaa atatccaaat atcctgttgt agatatggat
     2701 cgtttaaata agcagatttc tgatgtgtat gaaagacaag cagaagagct gattaacgaa
     2761 caggattatg tggaagcaga tcgtttattt cgtattgctt tacaatacta tccacagaag
     2821 aaagacagta ttacaaaccg attggaatcc ataactaatc tctttatcca aaaaggaaat
     2881 agtttactgg aagcaggaga ttatgaagct gcgttaattc actaccgtaa aacctttgat
     2941 ataattccag attatcctcc tgcggtagag gctatcaaac gcgtttatac tatccaggaa
     3001 aatattcgta aagctggcga actattttca caagcagaac ggctggaagc tacagg
//
LOCUS       DAORHB010000155         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_37562_length_3020_cov_5.227656, whole genome shotgun sequence.
ACCESSION   DAORHB010000155 DAORHB010000000
VERSION     DAORHB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37562_length_3020_cov_5.227656"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..2109
                     /locus_tag="PKM80_05000"
     CDS             199..2109
                     /locus_tag="PKM80_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNV93303.1"
                     /translation="MSLIQVINASIEFGGNYILSDINCTLEHNSKIGLIGSNGSGKTT
                     LIKLMLGVLLPSEGKVLHSKKCRIAYLPQNPVLDGNISFINYVHSSRPDILKLQKKIN
                     ELSELLQDNHSEANETELNDALEKYQAIGGDEFENDLKFIITSLGFTEDDYPKSLNLF
                     SGGEQSRICLARILMMSNDLLILDEPTNHLDVAMISWLEKYLNASNIPFLVVSHDRTF
                     LDNTVSVIFHLENKHLSITKGNYSSFEQAKAIELKTQERQYARQQKFIAETEDFISRN
                     IAGQKTTQAKSRLKLLSRMEVIQNPQQQKKIKLNIQSTGRSGNDVYTLKDVELGIFEN
                     APLAKNVNLEVHYRDRICIVGPNGCGKTTLLKILMEEKDIFSGSLKIGASLEIGYYDQ
                     HQVLLDEELTVMDTLWQLVPNETRGYVLSFLARFGFRGDDVDKKVCVLSGGEKSRLML
                     CTLIHQTPNLLILDEPTNHLDIAMNNSLLSALQEYRGTIVFVSHDRYFMKQLATKYWV
                     FHKALENDLLYPTISEVDMGLEQALELAFSIPELPKKAPVLRERKKKINPWHLEQIHK
                     QIEQEQATIEESNIKIENINSELALSSTYSNELRAKELCQQIELLLSNINKSKQRIAE
                     LENKYLELSYDS"
     gene            2099..>3020
                     /locus_tag="PKM80_05005"
     CDS             2099..>3020
                     /locus_tag="PKM80_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HNV93304.1"
                     /translation="MIPKRIGFTTSFPVEVLFAAGHIPVDLNNVFISQDSSNYVQNAE
                     LKGFPRTLCSWIKGNYIAALSSSLDMIIGIVVGDCSNSNSLLDMFTEVNIPIYRFSFP
                     ANRNPEDLDKEITKLENYFGVSRSDTLKAKKRLDEIRKKLLVLDEWTWKERLVSGLEN
                     HLWQVNSSDFGGNPDRYESELDMFLKEAEKRDPLPTKLRLAYLGVPPIYKNIYDTIID
                     LGGDVVFNEVQRQFAMPYLLPDIVQQYIAYTYPYSVFDRLKDIKPELEKRNIEAVISY
                     TQSFCHLQLDNLLLRKYIDLPFLTLEGDQPE"
BASE COUNT         1006 a          522 c          588 g          904 t
ORIGIN      
        1 gaccacttga accccattca agtacgctag ccggactgcg ctacatcccg aaccatttat
       61 taaccttaaa aattagacgg ggttttttga caagcaaaaa atgaggttga cagctaaagt
      121 gttttcctaa agtagattta attcagcttg ggatatgttt ttccgagttt taaataaaat
      181 atacaggaat attacaaagt gagcttaata caagttatca atgccagcat tgaatttggc
      241 ggcaactata tattgagtga tatcaattgc actttggagc ataatagtaa aatagggctt
      301 atcggttcga acggaagcgg taaaaccaca cttatcaaat tgatgttagg tgttttactg
      361 cccagcgaag gaaaggtgtt acattccaaa aaatgccgga ttgcttacct accacaaaat
      421 ccagttcttg atggcaatat ttcttttata aactatgttc atagttctcg accagatatt
      481 ctgaaattac agaagaaaat aaatgagcta tcagaattgc ttcaagataa tcattctgaa
      541 gcaaatgaga cggagcttaa tgatgcctta gaaaagtacc aggcaattgg tggtgatgaa
      601 tttgaaaacg acttaaaatt cataataact tctcttggct ttaccgaaga cgattatcct
      661 aaatccttaa atctcttcag tggaggagaa caatcccgta tttgtttagc taggatattg
      721 atgatgtcta acgatttact gattttggat gagccaacta accatctgga tgtagcaatg
      781 atttcctggt tagagaaata tctaaatgcc agtaacatcc cttttttagt ggtttcgcac
      841 gaccgtactt ttttagataa tactgtttcc gtgatctttc atctagaaaa taaacactta
      901 agcataacaa aaggtaatta cagctctttt gaacaagcaa aagcaattga actgaaaacc
      961 caggaaaggc aatatgcaag acagcaaaaa ttcattgccg aaaccgagga ttttataagt
     1021 agaaacatag ccggccagaa aactactcag gcaaaatctc gtttaaagtt gctttcacga
     1081 atggaagtta ttcaaaaccc tcaacagcaa aagaagataa aactaaatat ccaatcaaca
     1141 ggacgcagtg gtaatgatgt ttatacttta aaagatgtag aattaggcat ttttgagaat
     1201 gcacctttag caaaaaatgt taatctggaa gttcattatc gcgataggat ttgtatagta
     1261 ggtcctaacg gctgcgggaa aacaacgctg cttaaaattc ttatggaaga aaaagatatt
     1321 ttttctggtt cgctaaaaat tggcgcttct ttagaaatcg ggtattatga tcagcaccag
     1381 gttttattgg atgaagaatt aaccgttatg gacactctct ggcaattggt tcccaatgaa
     1441 acccgcggtt atgtattgag ctttttagct cgctttggtt ttcggggaga tgatgttgat
     1501 aaaaaagttt gtgttctaag cggaggtgaa aaatcgcgat tgatgctttg tacattaatt
     1561 catcaaacac ccaatttgct aattttggat gaaccaacaa atcatttgga tatagcgatg
     1621 aataatagtt tactatctgc tttacaagaa tatcggggca caattgtgtt tgttagtcac
     1681 gatcgctatt ttatgaagca actggccact aaatattggg tgtttcataa ggcattggaa
     1741 aacgacttgt tataccctac tattagcgaa gtggatatgg gtctggaaca agctttggag
     1801 cttgcttttt ccattccaga gctgcctaaa aaagctcctg tgctacgcga aagaaaaaag
     1861 aaaattaacc cttggcatct agaacagatt cataaacaaa ttgaacaaga acaggcaacc
     1921 atagaagaaa gtaatataaa aattgaaaac ataaattctg aattggcact ttcttccacc
     1981 tatagcaatg aattacgagc aaaagaacta tgccagcaaa ttgaactgct gcttagcaac
     2041 ataaataaaa gcaaacaacg cattgccgaa ttggaaaata aatatctgga gcttagctat
     2101 gattcctaaa cgcattggat ttactacttc ctttcctgtg gaagttttat tcgctgcagg
     2161 ccatattcct gtagatttga acaatgtatt tatatcacag gattcttcca attatgttca
     2221 gaatgctgaa ttgaaaggtt tcccccgcac tctttgcagc tggattaaag gtaattatat
     2281 agctgcgcta tcttctagtc tggatatgat aatcggaatt gtagtaggtg attgttccaa
     2341 tagtaattct ctactggata tgtttactga agttaatata ccgatatacc gtttttcctt
     2401 tcctgctaat agaaaccctg aggatttaga taaagaaata accaagctgg aaaattattt
     2461 tggtgtgtct cgaagcgata ctttgaaggc aaaaaagcga ttagacgaga ttcggaaaaa
     2521 actattagtc ctggatgaat ggacttggaa agaacgctta gttagtgggc ttgaaaatca
     2581 cctttggcag gttaattctt ccgattttgg gggaaatcca gatcgctacg aaagtgaatt
     2641 ggatatgttt ttgaaagaag cagaaaaaag agaccccctg ccaacaaaac tgcgtctggc
     2701 atatttggga gtgcctccta tctataaaaa catatacgat acgattattg atctgggagg
     2761 ggatgttgtt ttcaacgaag tgcagcgtca atttgcaatg ccttatttac taccggatat
     2821 cgttcaacaa tacattgctt atacttatcc ctatagtgtt tttgaccggt tgaaagatat
     2881 aaagcccgaa cttgaaaaac gcaatataga agctgttatt agctatacgc aaagtttttg
     2941 ccatctacag ttggataacc tgcttctaag aaaatatata gacctgcctt ttttaaccct
     3001 ggaaggtgat caacctgaag
//
LOCUS       DAORHB010000156         2989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_38149_length_2989_cov_4.672802, whole genome shotgun sequence.
ACCESSION   DAORHB010000156 DAORHB010000000
VERSION     DAORHB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38149_length_2989_cov_4.672802"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1930)
                     /locus_tag="PKM80_05010"
     CDS             complement(161..1930)
                     /locus_tag="PKM80_05010"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNV93305.1"
                     /translation="MNNITVKVGLASCGVAAGAKEVYNALKQYLKENDKPVTLKKTAC
                     IGMCFEEPIVQFEGSELGSIHIGKARPETIISILEDYIAGKAPAENIILAEKLPSRHN
                     DLLANQQRIVLRNCGIIDPECIEDYEARDGYKALQKALSMDRDAIIDEIKKSGLRGRG
                     GAGFPTGVKWQLCAKEVSAKKYVICNADEGDPGAFMDRSTLEGDPHSVIEGMIIGAYA
                     IGANEGYIYCRAEYPMALEHLKTAIAAAEKKNYLGKNILGSSFSFDLHIKEGAGAFVC
                     GEETALMQSIEGKRGMPTLRPPYPAAKGLWGKPTNINNVETWANVPWIIMHSANEYRK
                     YGTDKSPGTKVFALAGKIAGSGLIEVPMGIPLKDIIYKVGGGMKTSKPFKAVQMGGPS
                     GGCIPAEKIDTIVDYDSITATGAIMGSGGMVVMDTGTCMVDVAKFFLNFTQNESCGKC
                     TFCRIGTKRMLEILTRITEGKGELQDLNNLEELAENIIKGSLCGLGQTAPDPVLTTLR
                     YFKDEYLAHIVDKRCPAGVCTALITYKIDPNKCIGCTLCARKCPVSCISGKVKQVHTI
                     DLEKCIKCGACFDACKFEAVVKE"
     gene            complement(1923..2402)
                     /gene="nuoE"
                     /locus_tag="PKM80_05015"
     CDS             complement(1923..2402)
                     /gene="nuoE"
                     /locus_tag="PKM80_05015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNV93306.1"
                     /translation="MNNVLKRLDKIITTYQGKKGSLIPLLQETQNELGFLSRETMEYL
                     AEKMQIPAAEIFGVATFYSMFRLEPQGKHLIRVCKGTACHVSDADGIKKAVVDALQLA
                     EDENTTSDRQFTVMEVACLGCCSLAPVIMIDDRTYGKLVPEAIPAILHQYISETSNE"
BASE COUNT          890 a          651 c          482 g          966 t
ORIGIN      
        1 accaaacata cccaacagga accgtaagga ttcagttctt catcattaca taaagtagga
       61 atatcaatat tatgctgttg gcaaagttcc agaatagaga ttccggcttc cgctttcact
      121 gtttttccgt ttaaaattac ttctatcatt tccaccgctc ctattctttt actaccgctt
      181 caaatttaca ggcatcaaaa caagctccac atttgataca tttctctaaa tcaattgtat
      241 gcacctgttt cacctttccg gaaatacagg aaacgggaca ttttcgagca caaagagtgc
      301 agccgataca tttattggga tcaattttat aggttattaa agcagtacaa acacctgcgg
      361 gacagcgttt atctacaata tgagccaaat attcatcctt aaaataacgc aaggtggtta
      421 gaacaggatc aggagctgtt tgtcccaaac cgcaaagtga acccttgatt atattttctg
      481 ccaactcttc caggttgttt aggtcttgca attctccttt gccttccgtg atacgagtta
      541 agatttctaa cattcgtttg gttccaatac ggcaaaaagt acatttccca caggattcat
      601 tttgagtgaa atttagaaaa aacttggcta catcaaccat acaagttcca gtatccataa
      661 ctaccattcc cccggaaccc ataattgccc ctgtggcagt gatagaatca taatctacta
      721 tggtatcaat tttttcggca ggaatacaac ccccggatgg acctcccatt tgcacagctt
      781 tgaaaggttt ggaagttttc attcctcccc caaccttata gattatatct ttaagaggga
      841 tccccatagg gacttctatt aatccactac cagcaatttt acctgctaag gcaaatactt
      901 tagttccggg tgatttatct gttccatatt ttctatattc attggcactg tgcataatta
      961 tccaggggac atttgcccag gtttctacat tattaatatt ggtaggcttt ccccaaagtc
     1021 cttttgcagc aggatatggg ggacgcaaag taggcatacc tcttttacct tcaatggatt
     1081 gcattaaagc agtttcttct ccacaaacaa aagcaccggc accttccttg atatgcaaat
     1141 caaaactgaa agaactgccc aatatatttt ttcccaggta gttctttttt tctgcagcgg
     1201 caattgcagt ttttaaatgt tccaaagcca ttggatattc tgctcggcag tatatataac
     1261 cttcattagc accaatagca taagcaccaa ttatcatacc ttcaatcacg gaatgaggat
     1321 cgccttccag agtactacga tccataaaag cacctggatc accttcatca gcattgcata
     1381 taacatattt tttagcggat acttctttag cacaaagttg ccattttact cctgtaggga
     1441 aaccagctcc accgcgtcct cttaaacccg attttttgat ttcgtcaatg atggcgtctc
     1501 tatccatact tagtgctttt tgtaatgctt tataaccatc tcgggcttca taatcctcaa
     1561 tacattccgg gtctattatc ccacaattcc gtaaaacgat gcgttgctga ttagccaaaa
     1621 gatcattatg tctactcggt agcttttctg ctaaaatgat attctcagca ggagcttttc
     1681 ctgctatata atcttccaaa atactgataa ttgtttctgg tcgagctttt ccaatatgaa
     1741 tggagccaag ttcagaacct tcaaactgta cgatcggttc ttcaaaacac ataccaatac
     1801 aagcagtctt ctttaatgta acaggtttat cattttcttt aagatattgt tttagtgcat
     1861 tatatacttc ttttgcccca gcagctacac cacaacttgc caaacctact tttacggtta
     1921 tattattcat tagatgtctc gcttatatat tgatgtaaaa tagcaggaat cgcttccgga
     1981 accagtttgc cataagttct atcatcgatc ataattacgg gtgccaagga acaacatcct
     2041 aagcaggcaa cttccattac tgtaaattgt ctgtcggaag ttgtgttttc atcttccgca
     2101 agctgcaacg catccactac tgcttttttg attccatctg catcggaaac atggcacgca
     2161 gtccctttac aaacgcggat taagtgttta ccctgtggtt ccaaacgaaa catagagtaa
     2221 aatgttgcta ctccaaaaat ctccgcagca ggtatttgca ttttttctgc taaatattcc
     2281 attgtttctc tggataaaaa acccagttca ttctgtgtct cttgtaacaa aggtatcaga
     2341 gaaccttttt tcccttgata ggtagttata attttatcta aacgctttaa gacattattc
     2401 atatccaaac ctctatttct ccatatgtat cagacattac tccaaggtaa accatgtttt
     2461 gtagcaaccc atattttgat ggggcaggat atgtcaaaca aaaaaggtat acaaataatc
     2521 taaagtaagc acaggattgt ttattacgct atatcctatc atcaagataa ttatttcttt
     2581 attcattttg tggagaagat aagaagaatt actgtaactc ttttaggtat cgatgttatt
     2641 ttattccagg ttttctgttt agcattatca atccttacct gctttataat cgataacgat
     2701 gctaataatt agcgaacaga tctctagtca gactagggaa tcgtgagagg atttactaca
     2761 gagcaagtgt ttattatgct tgcattccta cagacatatt acacagctaa tattcggcat
     2821 ttatatgaaa acattaatgg ctactataat aatcccagcc ccaagaaaag acataaaaat
     2881 tgtagcagac aacctcaact ataatgagcg gaaagaggga gcttattttt tacaaacctc
     2941 aaattcccaa ctggaaatat agtttattct ttataacgag ggtggcctt
//
LOCUS       DAORHB010000157         2989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_38150_length_2989_cov_4.589639, whole genome shotgun sequence.
ACCESSION   DAORHB010000157 DAORHB010000000
VERSION     DAORHB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38150_length_2989_cov_4.589639"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /gene="thiC"
                     /locus_tag="PKM80_05020"
     CDS             complement(<1..996)
                     /gene="thiC"
                     /locus_tag="PKM80_05020"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNV93307.1"
                     /translation="MTQLQQATKGIATSEILTAAKEEHIEADILMQLIKEGKVVIPHN
                     QNRKAIPTAIGSKMTTKINANIGTSELCCEPDTEIAKLQLCKKYQAHSVMDLSTGGNL
                     DNIRIKMLEATDLILGTVPLYAVATELQRTDKDITAFEPEMLFAEIEKQAEQGVDFMT
                     VHAGINQWSLKAHQKENRLTGIVSRGGAFIKRWMLHTGKENPLYEQFDRLLNICLKHD
                     VTLSLGDGFRPGAICDATDCTQISELLVLSELVKRARLKGVQVMVEGPGHIPLPEIEA
                     NVLLEKKLCAEAPFYVLGPLPTDFACGYDHITGAIGGAIAASFGADFLCYVTPSEH"
     gene            complement(993..1646)
                     /locus_tag="PKM80_05025"
     CDS             complement(993..1646)
                     /locus_tag="PKM80_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNV93308.1"
                     /translation="MDNFGLYIVMTNPLLGYETFTEICVQEEVPMLQIRDKHLSDKEL
                     LNLCKRLKAITEGSKTRFIVDDRPDICLLTKADGLHLGQDDLPWKEVLPFLPTNTLLG
                     VSTHNLKQAKELMESITAQKNLQKPDYFSFGPIYPTVAKEKPDPAIGVEDLKTVLNLS
                     PIPVVAIGGLFPENLPNVLAVGAQNMAFIRHFTLSRETAELKAKIREMKSLLKEAQK"
     gene            complement(1649..2425)
                     /locus_tag="PKM80_05030"
     CDS             complement(1649..2425)
                     /locus_tag="PKM80_05030"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR00292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HNV93309.1"
                     /translation="MEAQISQAITSGWFTKLQNHLISDVIIVGGGPSGLVCAAELASQ
                     NIKTTLIEKDLAPGGGMWGGAMFFNQIVFHKDALQLLERFAISYSYYGNDLYVCDAVE
                     ATSSLIYNATHKGAGIFTGICMEDVLVKDNRVSGVVINWSTVKKDRLHIDPLSLSAKA
                     ILDATGHPCEVVSVLCRKNTIHLNLPSDTIPYEKSLNAEAGEKSCLEFTQEVYPGLFV
                     SGMAACGVSGSNRMGPIFTSMLLSGMKAADLILQSLNREN"
     regulatory      complement(2492..2590)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          854 a          602 c          615 g          918 t
ORIGIN      
        1 atgctcggag ggggttacat agcataaaaa atcggctcca aaggaagctg ctatagcacc
       61 tccaatggct cctgtgatat gatcataacc acaagcaaaa tcagttggca gaggacccaa
      121 cacataaaaa ggagcttcag cgcataattt tttctccagc aaaacattag cttcaatttc
      181 tggaagaggt atatgccccg gaccttccac cataacttgc actcctttta ggcgggctct
      241 tttaaccagt tcgctcagca caagcaattc tgaaatctgg gtacaatcgg tggcatcaca
      301 aattgctcca ggtctgaagc catctcccaa acttagtgtt acatcgtgtt tcaggcaaat
      361 atttagcagg cggtcgaact gttcataaag cggattttct tttcctgtat gtagcatcca
      421 gcgtttgata aaagcacctc ctctgcttac aattcccgtt aaccgatttt ctttttgatg
      481 tgccttaagt gaccattggt taatacctgc gtgcacggtc ataaaatcca ctccctgttc
      541 tgcctgtttt tctatttcgg caaagagcat ttcgggttca aaagcagtga tatctttatc
      601 ggttctttgt aattctgtag ctacagcata aagaggtacc gttcccaaaa ttaggtctgt
      661 tgcttccaac atttttatgc ggatattatc taaattgcca cctgtggaaa ggtccataac
      721 gctatgagct tgatattttt tacacagttg cagcttggca atttcggtgt ctggctcaca
      781 gcaaagttcc gaagttccga tattggcatt tattttggta gtcattttac ttccgatagc
      841 agttggtatc gctttacggt tttggttatg agggataacc actttgcctt cttttataag
      901 ctgcataaga atatcagctt caatatgttc ctctttggct gcggttaata tttcggaagt
      961 ggctatgcct ttggttgctt gttgtaattg ggtcattttt gtgcctcctt taataatgat
     1021 ttcatttctc taattttggc ttttaattcc gctgtttcgc gggaaagagt aaaatgcctg
     1081 ataaatgcca tattttgggc tccgacggct aaaacattag gcagattttc aggaaataac
     1141 cctccaatcg ccactaccgg tataggagat aaattcagaa cagtttttaa atcctcaacc
     1201 cctatagcgg gatcaggttt ttctttggct acagtaggat agatggggcc aaaactgaaa
     1261 taatcaggtt tttgtaggtt tttttgggca gtgatacttt ccattaattc ttttgcctgc
     1321 tttaaattat gcgtagaaac acctagaaga gtgttggtgg gcaaaaaagg taaaacctct
     1381 ttccagggaa gatcatcctg ccctaaatgt aagccatctg cttttgtcaa aagacagata
     1441 tccggtcggt catcaactat gaacctggtt ttagagccct cggtgatagc tttcaacctt
     1501 ttgcaaagat tcagcaactc tttatcggaa agatgtttat cgcgaatctg cagcatcggt
     1561 acttcttcct gaacacagat ctcggtgaat gtctcataac ccaaaagcgg attagtcata
     1621 acgatatata agccgaagtt atccattgtt aattctcccg gtttagcgat tgcagaatca
     1681 aatccgcggc tttcattccg gaaagcaaca tactggtaaa aataggaccc attctattac
     1741 tgccgctaac tccacaagca gccataccgg aaacaaaaag tcccggatag acctcttgcg
     1801 taaattccaa acaggatttt tcgcctgctt cagcatttaa agacttttca taaggaatgg
     1861 tatcagaggg caaattcaag tggatagtgt ttttgcggca taaaacactc actacctcac
     1921 aaggatgccc cgttgcatcc aaaatggctt tggcggataa agataaggga tcaatatgta
     1981 atctgtcttt ctttacagtt gaccagttta tcactacacc tgaaacccga ttatctttta
     2041 ccagtacatc ttccatacaa ataccggtaa aaatgcccgc tcctttatga gtggcattgt
     2101 aaattagaga ggaagttgct tccacggcat cgcaaacata taagtcgttg ccataatagc
     2161 tataggaaat tgcaaaacgc tctaatagct gaagcgcatc tttatggaaa acgatttgat
     2221 taaaaaacat cgctccaccc cacatcccgc ctccaggagc aaggtctttt tcaataaggg
     2281 tggttttgat gttttgcgag gcaagttccg cggcacaaac aagacccgaa ggacctcctc
     2341 caacaattat aacatcactg atgagatgat tttgcagctt tgtaaaccag ccgctggtaa
     2401 tggcttggga aatttgagct tccattttgt ccccttttta gttttttaag gggttgtttg
     2461 tatgagcttt atccttcgat ggttaactga gtgaaattta aggtcttctt cctcgcgccg
     2521 gtattatccg gatcaggttc gaagggtata atctcagccg gaatattttc cagcacccct
     2581 gttttaggtt acaaaaatac ttggcaccta aatgtcaagc aaaagataaa ataattaggg
     2641 gatgaacagg attatgattg cagggggcag agagctttgg gcgaaaagga ttctgtggga
     2701 ttttagattt gagttttagg aacaaggatt ttaggattag atttaagaaa caaggattta
     2761 aggattttag gattaagagg atttatttat aagccaccga aaccaccgaa atcaccgaaa
     2821 aaaatatcct cacgcagatc acgcagatca cgcagatata gtttttggaa caaggatttt
     2881 aggattagat tttagaaaca aggattttag gattttagga tttagaggat tgagttttag
     2941 aaacgaggat tttaggattt taggattaag aggatttcta atttaaagt
//
LOCUS       DAORHB010000158         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_38734_length_2959_cov_5.713499, whole genome shotgun sequence.
ACCESSION   DAORHB010000158 DAORHB010000000
VERSION     DAORHB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38734_length_2959_cov_5.713499"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKM80_05035"
     CDS             <1..689
                     /locus_tag="PKM80_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HNV93310.1"
                     /translation="EIQTGEDNYLTTSDAVEKAYTVTASQLINESFAILSGVKPEKMG
                     LGHAYEIDPELENSFSYELAHALLTKTLFPNSPVKYMPPTKFASGNIFHTYLLNAMFN
                     FVGQLTEQGVQLLGMMTEAIHTPHLADRSLAIENAKYIFRAIKGLNQDLCLTEDSFIA
                     KRANEVLAQATEFLEKVAQEDLFSAMAKGEFAEIKRPENSGKGLEGVIMKDSSYYNPF
                     MESMKQELGL"
     gene            686..1429
                     /locus_tag="PKM80_05040"
     CDS             686..1429
                     /locus_tag="PKM80_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HNV93311.1"
                     /translation="MSKNIIRPYGDTLNDGKMQLSFTLPVSCDALGKETARKLLLQMG
                     FDEAEITSMRDLTEGFTHFIAYAVTSTGVDISKIKVKVVETPVMTMEETDTYVAEHLG
                     RKITVVGACIESDAHTVGIDAIMNMKGYNHRYGLERYHSFNAINMGAQVTCEDLLAKA
                     REVNADAILVSQVVTQKNIHIKNLTRLIELAEAEGLRDKMLFICGGPRISFELAIELG
                     YDAGFGPGTYATDVASYIAITLAKQKAQE"
     gene            1455..2753
                     /gene="rseP"
                     /locus_tag="PKM80_05045"
     CDS             1455..2753
                     /gene="rseP"
                     /locus_tag="PKM80_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNV93312.1"
                     /translation="MLTLLITIIAFGLMIFVHELGHFLVARSFRVNIEKFSIGFGKPI
                     WETEKNGIQYSLGWIPLGGYVKMKGENPEESIYLEEDDTFVGKSWWKRALIAFSGPFA
                     NLLFGILLFIIAFMLPQKQEDLLPVIQNAKGIWAEHFSPADSIYSINGKAVTGFQDFL
                     LKLSDKKNNTIEYIHNGQKATLEIAPTQIDSLIRSLEPKVDTTIGEVFTGMPAWKGGL
                     KPGDKILAVDSVAVSNWYEMRERIIGSKNEEVQLTVLRNGKILICSIALEENVSMGNQ
                     KMIGITQYMPVKRVINYNPLEAISYGARSTLNFIVMNYVGLYKLIMKPEQLKNNVGGP
                     IMIATMGQQVAQRGFSSLIIFLASISLVLMIMNLLPIPILDGGHIFFAFFEGIFRKPV
                     PIKIQAFLQRIGFALLLLLMLYAFYADISKLLIRQLSLRQ"
     gene            2754..>2959
                     /locus_tag="PKM80_05050"
     CDS             2754..>2959
                     /locus_tag="PKM80_05050"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HNV93313.1"
                     /translation="MFNFTLQRTCGKARAGIIKTDHGEILTPVFMPVGTLGTVKAMSP
                     KELEEVQAQIILGNTYHLYMRPGHS"
BASE COUNT          962 a          457 c          632 g          908 t
ORIGIN      
        1 ttgaaattca gaccggtgaa gataattatt taactacttc cgatgctgta gaaaaagcat
       61 atacggttac tgcaagtcag ttgataaatg agagttttgc tattttaagt ggtgttaaac
      121 cggaaaaaat gggtttgggg catgcttacg agatagatcc tgagcttgaa aacagcttta
      181 gttatgaatt ggcacatgcc cttttaacaa aaaccctctt tcctaattca ccggttaaat
      241 atatgcctcc aacaaagttt gccagtggca atatatttca tacctatttg ctgaatgcga
      301 tgttcaattt tgttggtcag ttaacagaac agggagtaca gcttttagga atgatgacgg
      361 aggcaattca tactcctcat ttagcagatc gctctttagc tatcgaaaat gcaaaatata
      421 tatttagagc gattaaggga ttaaatcaag atttatgttt gacagaagat agttttatcg
      481 ccaaaagagc taatgaagtt ttggcacagg caacagaatt tttggaaaaa gttgcccaag
      541 aagatctttt tagtgctatg gctaagggcg aatttgcaga aattaagcgt cccgaaaaca
      601 gcggtaaagg tctggaaggt gtaattatga aagatagttc atattacaat ccttttatgg
      661 aaagtatgaa acaggagctt ggattatgag caagaatata attcgtccct atggagatac
      721 cttaaatgat ggcaaaatgc agctttcttt tacgctgcca gtttcgtgtg atgctttagg
      781 aaaagaaaca gccagaaaac tactattaca aatgggtttt gatgaagcag aaattactag
      841 tatgagagat ttaactgagg gtttcactca ttttattgct tatgctgtta catccacagg
      901 agtagatatc agcaaaataa aagtaaaagt agttgaaact ccggttatga ctatggaaga
      961 aaccgatacc tatgtagcag aacacttagg aagaaaaatt acagtagtag gcgcctgtat
     1021 tgaaagtgac gcacatacag ttggaattga tgctataatg aatatgaagg gctataatca
     1081 tcgttatggt ttagaacgct atcacagttt taatgctata aatatgggtg cacaagttac
     1141 ctgtgaagac cttttagcca aagccagaga agtgaatgca gatgcaattt tagtttcgca
     1201 ggtagttacg caaaagaata ttcacatcaa aaacttaact cgcttgatcg aacttgcaga
     1261 agctgagggt ctgcgtgata agatgctgtt tatttgtggt ggaccgcgta tttcttttga
     1321 gcttgctatt gaacttggtt atgatgcagg ttttgggcct ggaacttatg ctacagatgt
     1381 tgcaagttat atagctatta cattggcaaa acaaaaagct caggaatagg caatcaggat
     1441 ttaagcgtaa cattatgcta acattattaa tcacgataat tgccttcggg ctaatgatct
     1501 ttgtgcatga attaggtcac tttttggtag ctcgctcatt tcgggtgaac attgaaaaat
     1561 tctctatagg ttttgggaag ccgatttggg aaactgaaaa aaacggcatt caatacagtt
     1621 taggatggat tcctttgggt ggttatgtaa aaatgaaagg agaaaatcca gaagagagta
     1681 tctacttaga agaagatgac acttttgttg ggaaatcttg gtggaaaaga gctctcatag
     1741 ctttcagcgg accgtttgct aatttgcttt ttgggatctt actttttatt attgccttta
     1801 tgttgccgca aaaacaagaa gatcttttgc cagtaattca aaatgctaaa ggaatttggg
     1861 cagaacattt cagtcctgca gatagcatat atagcattaa tggtaaagct gttacaggtt
     1921 ttcaagattt tctacttaag ctatccgata aaaaaaataa tactatagag tatattcaca
     1981 atggacaaaa agccactttg gagattgctc ctacacaaat agattcttta attaggtctc
     2041 ttgagccaaa agtggatact acaattggag aagtttttac tggaatgcct gcttggaaag
     2101 gaggtttaaa accaggtgat aaaattttgg ctgtagatag tgtagcagtt tccaattggt
     2161 atgaaatgcg cgaacggata attggctcta aaaatgagga agttcaactt actgttttac
     2221 gcaatggaaa gattttgatc tgcagtattg ctttggagga aaatgtctct atgggaaatc
     2281 aaaaaatgat tggtatcacg caatatatgc cggtgaaaag agttatcaat tataatcctt
     2341 tggaagcaat tagctatggc gcaaggagca cattgaattt tatagtgatg aattatgtgg
     2401 gcttatacaa actgattatg aaaccagagc agttaaaaaa caatgttggg ggacctataa
     2461 tgattgctac aatgggtcaa caagttgctc aacgaggttt tagttcccta atcatctttt
     2521 tggcttccat tagtttagtt ttgatgataa tgaatctatt gccaataccg attcttgatg
     2581 gtggacatat attctttgcc ttttttgaag gtattttcag aaagcctgta ccaattaaaa
     2641 ttcaggcatt tttacagaga attggttttg ccctactgct attattgatg ctatatgctt
     2701 tctatgcaga tatatccaag ttgctaatcc gtcagttatc attgaggcaa tagatgttca
     2761 attttactct tcaaagaact tgtggaaaag caagagcagg aataataaaa acagatcacg
     2821 gtgaaatact gaccccagtt tttatgccgg tgggcacact gggaacagtg aaagcaatga
     2881 gccccaaaga attagaagaa gttcaggcac agataatctt aggcaatacc tatcatttgt
     2941 atatgcgccc cggacattc
//
LOCUS       DAORHB010000159         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_39467_length_2923_cov_5.307183, whole genome shotgun sequence.
ACCESSION   DAORHB010000159 DAORHB010000000
VERSION     DAORHB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39467_length_2923_cov_5.307183"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1063)
                     /locus_tag="PKM80_05055"
     CDS             complement(68..1063)
                     /locus_tag="PKM80_05055"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNV93314.1"
                     /translation="MSKTYNAKFSSFGSFTPAKILNNFDLEKIVDTSDEWIRTRTGMF
                     ERHIASENEAASDLAYQAAVRAIESSSVKYKDIDHIIIATISGDHPFPSTACVIQKKL
                     GLKNIPAFDISAGCTGFIYAIDVARQYVENGAAQNILVIGVEILTKITNWKDRNTCVL
                     FGDAAGAAIISRAESSEISLIIDSLIEADGSQGEYLIQKAGGSRMPASHETVDQNLHT
                     VYMEGNRIFKSAIKCMYAACTEILARNDLKVADIDWIIPHQANLRIIEALASKLKVPM
                     SKIIVNIEKYGNTSSATIPLAVDEAIRAQKIRRGDKILLTSFGAGLTWGSVLARY"
     gene            complement(1066..2061)
                     /gene="plsX"
                     /locus_tag="PKM80_05060"
     CDS             complement(1066..2061)
                     /gene="plsX"
                     /locus_tag="PKM80_05060"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNV93315.1"
                     /translation="MRIAVDAFGTDNAPYPEIEGAVLAIKEDICDEVILVGDEQIINK
                     ELGKFFYPQNRISVVNASQRIEMEDSAAAAIRTKKDSSLVRAIELHKQGLADAVVSAG
                     NTGAVMAASLLIYRCIKNVQRPAIAVVFPTQEGHQILLDMGANVDCSAENLLQFAKLG
                     SMYFEYFFQTKNPRISLLNIGEESAKGNATTKQAYKLLSQDKELNFTGNIEGKDIIRG
                     ITDVVVCDGFVGNIVLKTVEGVGFSIFEILKEEINKDWIAKIGSLLSYPVYSYIKNKL
                     DHTEYGGALLVGLNGISVVAHGRSNAKAIKNAIRFADRIAKSGFVTHAKAYFERF"
     gene            complement(2063..2242)
                     /gene="rpmF"
                     /locus_tag="PKM80_05065"
     CDS             complement(2063..2242)
                     /gene="rpmF"
                     /locus_tag="PKM80_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNV93316.1"
                     /translation="MAVPKRRTSKMRRDKRRAHRALTIPAFSTCKKCAEPVRPHHICE
                     KCGTYNGKKILAEKE"
     gene            2687..>2923
                     /locus_tag="PKM80_05070"
     CDS             2687..>2923
                     /locus_tag="PKM80_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93317.1"
                     /translation="MARQRTLVNLVSKSKVHKKEVPHFCFNAMMKGSKKDMLVLYEQL
                     SLGKDKKRFDSLAIMKFGDSNYRLRPKRKAQVPYF"
BASE COUNT          939 a          612 c          474 g          898 t
ORIGIN      
        1 cccaatatac tgagcacctt gacctggaaa aacaaatgca gtcttcataa tttaacctct
       61 tataaaccta atatcttgcc agaacactac cccaagttaa acccgctccg aaagaagtaa
      121 gcaatatttt atcgccgcgt cttattttct gagcgcgaat tgcttcatca acagccaaag
      181 gaatcgttgc tgatgaagtg ttcccatatt tttctatatt cacaattatc ttactcatcg
      241 ggacctttag cttagaagcc aaggcctcaa taattcttaa atttgcctga tggggaataa
      301 tccaatcaat atcagccacc ttaagatcgt tgcgcgccaa aatttcagtg caagcggcat
      361 acatacattt tatagcactt ttaaatatcc gatttccttc catataaaca gtatggagat
      421 tttggtctac tgtttcatgg ctggcaggca ttcttgaacc accagctttt tgaattaaat
      481 attcaccctg gctaccatct gcttcaatta aactatcgat tatcaaagat atttcactgg
      541 attcggctct actgataata gcagcaccag ccgcatcacc aaatagaaca caagtattac
      601 gatctttcca attagtaatc tttgttagaa tctctactcc aatcaccaga atattttgcg
      661 ccgcaccatt ttctacatac tgacgcgcta catctattgc atatataaaa cctgtgcaac
      721 cagcagaaat atcaaaggct ggaatattct tcaaacccaa ttttttctga atgacacaag
      781 cagtagaagg aaaaggatga tcaccagaaa tggtagcaat aataatatga tcaatatcct
      841 tatatttaac cgatgaactt tctatggctc taaccgcagc ttgataagcc aaatcactgg
      901 ctgcttcatt ttcagaagct atatgacgtt caaacattcc tgtgcgggtt ctgatccatt
      961 catcactggt gtccacgatt ttttccaaat caaaattatt aaggatctta gcgggggtaa
     1021 aactaccgaa ggaagagaat ttagcattgt aagttttaga cataattaaa acctctcaaa
     1081 ataggccttg gcatgtgtaa caaagccaga tttagcaatc cgatcggcaa aacggatagc
     1141 attttttatt gcttttgcat tactgcgtcc atgagcaact actgatatac catttaaacc
     1201 aacaagtaat gctccaccat attcagtatg gtctagttta ttttttatgt aggaatagac
     1261 aggataggat agcaaagatc caattttggc gatccaatcc ttattaatct cttcctttag
     1321 tatttcaaaa atggaaaaac ctacaccttc caccgtcttt agaacaatat tacctacaaa
     1381 tccatcacat acaactacat ctgtgattcc gcggataata tccttacctt caatattacc
     1441 agtaaaatta agctccttat cctgactgag caatttatat gcctgcttgg ttgtagcatt
     1501 gcccttcgca ctttcttctc ctatattcaa aagcgaaatg cggggatttt ttgtttgaaa
     1561 aaaatactca aaatacatac tacccagctt ggcaaactgt aataaatttt ctgctgaaca
     1621 atctacattg gcacccatat ccaataatat ctgatgccct tcctgtgtag gaaaaacaac
     1681 tgctatggca ggacgttgaa cattttttat acacctataa attaaaagag atgcagccat
     1741 tactgcacct gtatttcctg cactgacaac agcatcagca agaccttgtt tatgaagttc
     1801 aatcgctctt actaaagatg aatccttttt tgtccgaatt gcagctgctg cactatcttc
     1861 catttctatt ctctgactgg cattcacaac gcttatcctg ttctgaggat aaaagaattt
     1921 acccaattct ttattgatta tttgttcatc tccaaccagg ataacttcat cgcaaatgtc
     1981 ctctttgata gccaaaacag caccttctat ttctggatag ggggcattat ctgttccaaa
     2041 agcatcaaca gcgatgcgca ctttactcct tttctgccaa tatcttcttg ccgttatagg
     2101 tgccacattt ttcgcaaata tgatgcggac gaacaggttc agcgcatttt ttgcaagtac
     2161 taaaagccgg aatagttaaa gccctatgtg ctctgcgttt atctctacgc attttggaag
     2221 ttcttctttt gggaacggcc attattattc tccttaagct aatttcttat aagcattaca
     2281 caagcgataa cataagatca gaagttaacg atttccccta taagttaatt atgcgcttga
     2341 gctttatata aatttagagg ataaactttg agaggactga ttttgtcaac caaaatatat
     2401 gagcagcggt ttattccttt gtgcaaaaag cagatagaga gctaattaag ataactaaaa
     2461 cgatatcaaa aagataaact ggaagggcta ttattatgat cgagcatatt cagttcttca
     2521 aaaaactaat atacaataac ttacagctct ccagcacttc ttccctaaat agctatcggg
     2581 gaattgtggt gcaactgatt agcaaaatcc cagagttatc ccttggtagc tgcaccatat
     2641 ataactaagg aaggagtaaa aaaaatatag tggtaaagga gaataaatgg ctaggcagcg
     2701 caccttggtt aatttggtat ctaagtctaa agttcataaa aaagaagttc cacatttctg
     2761 ctttaacgca atgatgaaag gaagcaaaaa ggatatgcta gtgctatatg aacagttatc
     2821 tttgggaaaa gacaaaaaaa gatttgacag tttagccatt atgaaatttg gtgattcaaa
     2881 ttatagatta cgtcctaagc gtaaagcaca agtcccatat ttc
//
LOCUS       DAORHB010000160         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_40202_length_2892_cov_4.432499, whole genome shotgun sequence.
ACCESSION   DAORHB010000160 DAORHB010000000
VERSION     DAORHB010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40202_length_2892_cov_4.432499"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /gene="alr"
                     /locus_tag="PKM80_05075"
     CDS             <1..1688
                     /gene="alr"
                     /locus_tag="PKM80_05075"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV93318.1"
                     /translation="PEEGRLLRLQGCKSPILILSPCLTSEINVILDNDLAVNVTSLNF
                     AKALNKAAVVKDKQAIIHLKVDTGMHRSGILDSEFSDFYSQIQKLSNLNIEGVFSHFA
                     SADDDIEFSNQQEERFWKVIDQLPERPKYIHIDNSHALINKLGKRSNLVRLGIMAYGI
                     QIPLQEDLGLQPVMTFKTTLSQIKHISKGESIGYNRSWIADKDCVYGILPIGYADGYD
                     FMLSNNGVVWLDNVLCKVIGRISMDMICIDLSDIDKPKIGDVATLLGGSRLETRAENL
                     VAKYGGNSYELLCQVGRRAKSYFFQNGKLIHSIPLLRRDFVPDDFPDSKLNKIIESAI
                     AQRLQSIEIGEIIYREMLRDFFYYKDRDIHYRHNFHHKIVFSSSLIAGYYNASTILNF
                     DKVLSNDYFLVVCASSDEVLRRYFKNGDVEYRWLMDDSFKLDNESFIVCLVQVDELIL
                     ETSIKHINDCLEIKCSHPKLRGKIGKKVHFTINTQTLYPTSAHQFSVFITELTRGVDI
                     TFQYPQEIKEVDCVPFFSGQDKDPLITQNNREIRVSTELNEWIFPISGVVFAY"
     gene            1786..2736
                     /locus_tag="PKM80_05080"
     CDS             1786..2736
                     /locus_tag="PKM80_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93319.1"
                     /translation="MKRLNLLLMLILCIPLLAGSFNWYSQNDERWKSERLGSGNSSIG
                     SSGCVLSCLSMLLNAEASNQYMTPDRLNEWLQKNGGYAGNNMRWQIPGEIDGSGLGLE
                     LVAQSTKRNDWDFLSGELEKGNKVIVKVNGRRSHWVLVIKQNGPKDNPSSYIINDPGI
                     KEYEERTLAYFGGFKSARSYSGNWLDEQAFDLDSEINVVPVPQDELFLYDVAKLPHPA
                     DVYVTLHNNLPVDITGYFILGLFDEDGNYIRTVDYEYVTVEASGKYDLLYELSDVKPL
                     NEEHQELIIIYSKYFSAMPSLTESLELISKGIRNYTTITN"
BASE COUNT          951 a          455 c          538 g          948 t
ORIGIN      
        1 atccagaaga aggccgttta ttaagacttc agggatgcaa atctccaatt ttaattcttt
       61 ctccctgttt aaccagcgaa ataaatgtta ttttagacaa tgatttggcc gttaatgtta
      121 cttccttgaa ttttgccaaa gctctaaata aagctgctgt agtaaaagat aaacaggcaa
      181 ttatccattt gaaagtagat acaggtatgc atcgcagcgg aattctggat agcgaatttt
      241 ctgattttta ttcccagata caaaagctga gcaaccttaa tatagaaggg gtattcagtc
      301 attttgcctc tgcagatgat gacatagagt ttagtaatca gcaggaagaa agattctgga
      361 aagtgataga ccaacttcct gaaagaccaa aatatattca tatagataat tcccatgccc
      421 taataaataa attgggaaaa cgaagtaatt tagtacgatt aggcattatg gcttatggaa
      481 ttcagattcc tttgcaagaa gatctcggtt tacagccagt tatgactttt aaaactactc
      541 tctcacaaat aaagcatatc tccaagggtg aatctattgg ttataatcgt tcctggatag
      601 ctgataaaga ttgtgtttat ggaattctac ctattggata tgcagatggc tatgatttta
      661 tgctttccaa taatggtgtt gtttggctgg ataatgtact ttgtaaagtt attggccgaa
      721 tttcaatgga tatgatatgt atcgatttaa gtgatataga taaacccaaa atcggagatg
      781 tggcaactct cttaggtggt tcaagattag aaacaagggc agaaaattta gttgccaaat
      841 atggaggaaa ttcttatgaa ttactttgcc aggtgggacg acgcgcaaaa agttattttt
      901 ttcaaaatgg aaaattgatt cacagcatcc cattattaag gcgagatttt gtcccagacg
      961 attttccgga ttcaaaactg aataaaatta tcgaatcagc aattgcccag cggttacaga
     1021 gcatagaaat tggagaaatt atctatcgtg aaatgttaag agatttcttt tattataagg
     1081 atagagatat ccattaccgc cataatttcc atcataaaat tgtcttttct agttctttga
     1141 ttgctggtta ttataatgca tctactatat taaatttcga caaagtgctc agtaacgatt
     1201 attttttagt tgtttgtgca tcctctgatg aagtcttacg aagatatttt aaaaatggag
     1261 atgtagaata cagatggctt atggatgata gttttaaact tgataatgaa agctttattg
     1321 tttgtctggt tcaggtagat gaactcatct tggaaaccag tatcaagcat ataaatgact
     1381 gtttagaaat taaatgcagc catcctaagc taaggggaaa aataggtaaa aaagtgcatt
     1441 ttactatcaa cacccaaacc ttatatccaa cctctgctca tcaattcagt gtttttatca
     1501 cagaactcac taggggagta gatatcactt ttcaatatcc acaggaaatt aaagaagttg
     1561 attgtgttcc ttttttttca ggacaagata aagatcccct tattactcaa aataatagag
     1621 aaataagagt gtctacagaa ttgaatgaat ggatatttcc tatcagtgga gttgtttttg
     1681 cctattgaaa cataaaaaaa gattgacaaa tcttcctcgt cagaattgta gtttctccat
     1741 tataattatt tatatttgtt gttgttatct aacggtgact tgcttatgaa aagattgaac
     1801 ttattattaa tgttaatttt gtgcatacca cttttagctg gaagctttaa ctggtattca
     1861 caaaatgacg aacgctggaa atcggaaagg ttagggtctg gaaatagttc cataggtagt
     1921 agcgggtgtg tgcttagttg tttatctatg cttctaaatg cagaggcaag taatcagtat
     1981 atgactcctg atcgcttaaa cgaatggtta caaaaaaatg gtggctatgc tggaaacaat
     2041 atgcgttggc aaattccagg tgaaattgat ggcagtggat tggggctcga attagttgct
     2101 caaagcacaa aacgtaacga ttgggatttc ctttcgggag aattggaaaa aggcaataag
     2161 gttattgtaa aagttaatgg tagacggagc cactgggttc ttgttatcaa acaaaatggc
     2221 cccaaagata acccgtcttc ctatataata aatgatcccg gcatcaaaga atatgaagaa
     2281 aggacccttg cctattttgg tggcttcaaa tcggcgcgtt cttattccgg taattggtta
     2341 gatgaacaag ccttcgattt ggatagtgaa attaatgtcg ttcctgtacc tcaagatgaa
     2401 ctatttcttt atgatgttgc taaattacct catcctgccg atgtttatgt aactttacat
     2461 aataatttac ctgtcgacat tactggatac tttattcttg ggctttttga tgaagatgga
     2521 aactacatcc gtactgttga ttatgaatat gttacagtag aagccagtgg caaatacgat
     2581 cttttatatg aactatccga tgtaaaacct ctaaatgaag aacaccaaga attaataatc
     2641 atttatagta aatacttctc tgctatgcct tctttaactg aatcccttga gttgataagc
     2701 aaaggcataa gaaactacac aactatcaca aattaaagct tataactaca tattattatc
     2761 aagttctata ataagcgaac tgatttttga ttttttctat acgcacattt ctgttaatat
     2821 tttcaaacta aaatctaaat tatgttttct gccaggcaac atcgtataac tcgtaaagag
     2881 tcatataatt cg
//
LOCUS       DAORHB010000161         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_40638_length_2871_cov_7.227273, whole genome shotgun sequence.
ACCESSION   DAORHB010000161 DAORHB010000000
VERSION     DAORHB010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40638_length_2871_cov_7.227273"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1453
                     /locus_tag="PKM80_05085"
     CDS             311..1453
                     /locus_tag="PKM80_05085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNV93320.1"
                     /translation="MEAFSFCNPTRIEFGTGKIKILGMELHKAGIKSCLMVAGGGSIK
                     ANGVYLQVCESLNKSGIRFVESWGVQSNPTLQKVNELCELAKEEKVEAILAVGGGSVI
                     DTAKTVAAGVYLQNIWNAFLGKERIRNALPIYTVLTISATGSEMNGNAVITNNETLQK
                     WSIYSPLIYPHLSIIDPSVQSSLPFKQTANGAMDAIAHILEFYFADDRAIATLAIDDA
                     LQKTIIEMTDRLQLNSGDLLARGNLAWCATLALNGISGIGMKGGDWACHSIEHSFSAL
                     HPDIAHGEGLGVIFPAWIEYMSEKDPSRFLRWAKNVWDEENVSRALHRFRDKLESWGM
                     AQTLRDLGIKENELPQIVNNIMSSPRIGMVSKLSAVEVESLLMLAY"
     gene            2006..2812
                     /gene="istB"
                     /locus_tag="PKM80_05090"
     CDS             2006..2812
                     /gene="istB"
                     /locus_tag="PKM80_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNV93321.1"
                     /translation="MISQLQQGLRSLKCYHMANVLEEVVQISKENDLSYLDCLNRLVE
                     YELKNRKLNRIKIQFRRAGLPYEKFLEEFDFKYQTSISKRQVNGWLDFSWLDNRENKL
                     FLGPSGVGKTHLAIGLSREALQKGYKVRYFSMCDFVEEMIIKAASSSSKGWLKELLRN
                     DLIVLDEIGYLPIDPRYTHLFFQFINECYEYRSVLITSNKMPAQWGSYFGDESVAMAI
                     LDRLMHHSEIVILNGDSYRLKDKLDTLKASEKFSDGAFPLRSKAPSENFN"
BASE COUNT          913 a          495 c          632 g          831 t
ORIGIN      
        1 gagcgaatgg ttcgcaatca ttaggccagg ggttcgaatc cccttagctc caccataatt
       61 ttttctgctt aataagaata agtctatctc ctatagcctt tctggctgta tgccgagttc
      121 aaatttcaat aattatctct taatctcgtt taggaactgt tagagaagct cctaaacagt
      181 aaaaaatttc agcttttaaa atggaatcag gggaaaaatg ggcattttta cttgttttat
      241 tacagatttg cgttattttg tatttagatt ctgaacaaca agcacaaaaa ggaatatgaa
      301 ggagatagaa atggaggcct ttagtttttg taatcctacc cggattgaat ttggaacggg
      361 aaagattaaa atattgggaa tggaactaca caaagcgggt atcaaaagct gcttgatggt
      421 agctggcgga ggttcaataa aggcaaatgg cgtttatctt caggtttgtg aatcgttaaa
      481 taaatccgga atccgtttcg ttgaaagttg gggggttcaa tctaatccta ctttacaaaa
      541 ggttaatgaa ctctgtgaac tggcaaaaga agaaaaagtg gaagccattc ttgctgttgg
      601 aggtggaagt gtaattgata ctgcaaagac cgttgctgct ggtgtatatt tacaaaatat
      661 ctggaatgct tttttaggaa aggaacgaat tcgtaatgct ctaccaattt atacagtttt
      721 aacaatttcc gccacaggca gcgaaatgaa tggaaatgcc gttattacta ataacgaaac
      781 cctacaaaaa tggagtattt attccccgtt gatctatccc catttaagta ttatagaccc
      841 ttctgtgcaa agtagtttac ctttcaaaca aactgctaat ggagcaatgg atgccattgc
      901 tcatatttta gaattttact ttgccgatga tcgagctatc gctaccttag caatcgacga
      961 tgctctgcaa aaaacaatta tagaaatgac tgaccgcctg caacttaatt ccggggattt
     1021 actagccaga ggaaatttgg catggtgcgc tactttggct ttaaatggaa tttccggaat
     1081 cggaatgaag ggaggcgact gggcttgtca ttcaatagaa catagtttct ctgctttaca
     1141 tccagatatt gctcatggtg aaggtttagg agttattttc cctgcttgga ttgaatatat
     1201 gagtgaaaaa gacccctctc gctttttacg ctgggctaaa aatgtttggg atgaagaaaa
     1261 tgtttcccgc gctctacata gatttcgcga taaattggaa tcttggggaa tggcacaaac
     1321 tttgcgagac ctgggtatta aggaaaatga actgccacaa atagttaata atattatgag
     1381 tagtcccaga atcggaatgg ttagtaaatt atctgctgtg gaagtagaat ccttattaat
     1441 gctggcatat taaattacat attgcagctc ttatattaga taacagtaac ttctttatct
     1501 ttcctcagca ttaggaaagt acaccatata aattatacat aaaaaagcaa tcgccgaaac
     1561 ggtattggat aatctgtgcg gtaaacatat ctaatacctt atccttgtta gcttctaccc
     1621 aatgtatata gatgtgattt aaccaaaatg aatttatggc gagtagaggg tataagtacc
     1681 cccctaccag agataataaa tgtcactaac ccaaaaatgg caaaagcaca gaaagggaaa
     1741 atgtaagaaa tgtaaagata agcaggaagt actaaaacca aagaaagtac ttagccaatt
     1801 ccaaatagat atacaatatc aagcagttca ttttattatt ctgtgttgag ctgtaagaag
     1861 gtcgagcgct ttctggagct gagacgcagt cagggtgaac gaggcaaagc caagtatgca
     1921 gttccacaga cctccgtatg tatcaaggaa aagaacagat tccgggactt gagttactat
     1981 gatcaaatcg caggaggtct acaccatgat tagtcaattg cagcaaggtt tacgcagttt
     2041 gaagtgctat catatggcaa atgttttgga agaagtagtt cagatcagca aggaaaatga
     2101 tttatcctac ctggattgcc ttaatcggtt ggtggaatat gaattgaaga acagaaagct
     2161 gaaccggatc aagattcagt tccgaagagc tggcttacct tatgaaaagt tcctggaaga
     2221 atttgatttt aagtatcaga catcgattag taagcgtcaa gtaaatggtt ggctggattt
     2281 tagttggctg gataatcggg aaaacaaact ttttttagga ccctctggag taggtaaaac
     2341 gcatttagcc ataggactaa gccgggaagc acttcagaag ggttataagg ttcgttactt
     2401 cagtatgtgt gattttgtag aagagatgat catcaaggca gcaagttcga gctccaaagg
     2461 atggctaaaa gagcttctca gaaatgacct gatcgtgctt gatgagattg gttatctacc
     2521 gatagatccg cggtatacgc atttattttt tcagttcata aacgaatgct atgaatatcg
     2581 ctccgtcctc atcacatcga ataaaatgcc ggcacaatgg ggtagctatt ttggtgatga
     2641 gtcggtagcc atggcgatct tggatagatt gatgcaccat agtgagatag tgatcttgaa
     2701 tggtgacagc tatcgtctga aagacaaact ggatactctc aaagcgagcg aaaagttctc
     2761 cgacggcgct tttccgctac gctccaaagc gccgtcggag aactttaatt gagatagtgt
     2821 gtggtgcact ttaaagtgcc aaaactggtg cacattaaaa ttccgttgac a
//
LOCUS       DAORHB010000162         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_40934_length_2858_cov_5.431324, whole genome shotgun sequence.
ACCESSION   DAORHB010000162 DAORHB010000000
VERSION     DAORHB010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40934_length_2858_cov_5.431324"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PKM80_05095"
     CDS             complement(<1..998)
                     /locus_tag="PKM80_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNV93322.1"
                     /translation="MHKIKSMCFIIGCLALVIMLSGCLQKVEKTPVNYYVLEYQPGSE
                     KPELRRANNTGKNLEVLDTVLPRTYDRNQIVVKENFYKVKFLQTDVWATRLRDAIPNL
                     VVQRVKAYNIFGTVSRGEILDKEPHYFLETTVYNIEKIEGAEPRAYLKMEFVLRDSTS
                     ERIVLSHKNERSVELIDPSMIYLVQAFNDMIMEETDLFAAKCNLYFSGHKIDEKSLAT
                     SASPMARYVYEEMFASETVNDYGELMVITKTQTEEELRYTIEELDSLNSVISTDELVM
                     GVPALLRPGRYRVIIGEMEDLIIPVQILPRQRTVVKPNWGELKIVIMDESKSRVRMG"
     gene            complement(1007..2239)
                     /locus_tag="PKM80_05100"
     CDS             complement(1007..2239)
                     /locus_tag="PKM80_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV93323.1"
                     /translation="MVSKAAKIRLGVFLTIGAILIIIFAAIVAGSRLVEKKDIYYIEF
                     EDYSVSGLQVGSSVNYRGIKIGRVEDIKINPKDVTKIIVSISVNRGTPIKEDTEAVLL
                     LSGITGLKSVEIRGGTNEAKLLKPKSYIKPGTTMLEDISERAKSIVDKIDMIAANLNL
                     ITGEENRQNISKILEQTSLILKDTHENLATTMQSISRIANNTADLTEELSRDLSAVTD
                     NLTKNLDAITNSATSNIQSVSETSQTSLISVTSNVNKQLEELTSQLEYSLARLTEDGS
                     ALIREANLKVTTVGEHSDQMILETTREILTISNNINKTLDQVNAILYTPGVDSLMTNL
                     GTLCGELSRANINEMVSELSTTIHKTGNLVANLNRVVTRGQGDLLEILSTLTETSENL
                     NEFSRQIADTPAILLRGN"
     gene            complement(2242..>2858)
                     /locus_tag="PKM80_05105"
     CDS             complement(2242..>2858)
                     /locus_tag="PKM80_05105"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNV93324.1"
                     /translation="QCSDNDLPRFQDIEYYKYFNTNNLWIDLRALEWYIIANEGLLLL
                     PLIVNPKIVEGTPVYQLETAMGSAISIFNNSKALIVPRERFAPVKKTNDLLALWSDAY
                     DLNDMYQVVLKRGLEKPPVIELDEIYYGKIDDMQKRFSKGIPCLSGCKELKIYGDVSF
                     GEGVICEGKTTLKASNPVFVQNCLLTGEVNFDMQRNFPQDKAGG"
BASE COUNT          833 a          622 c          433 g          970 t
ORIGIN      
        1 cccattctta ctctgctttt ggattcgtcc ataatcacaa tttttaattc gccccaattg
       61 ggtttaacca ctgttctttg acgaggtaaa atctgaacag ggataattag atcttccatt
      121 tccccaatta ttactctata tctacccgga cgcagtaaag ctggcactcc cattactagt
      181 tcatctgtag agataacaga attcagcgaa tccaattctt cgattgtata acggagctct
      241 tcttcagttt gggttttagt aataaccatc aactccccat aatcattaac agtttctgag
      301 gcaaacattt cttcgtaaac atatctagcc attggtgaag cagaagtagc aagagatttt
      361 tcatctattt tgtggccact gaagtacaga ttacatttgg ctgcaaagag atctgtctct
      421 tccataatca tatcattaaa tgcttgaacc aaatatatca tagaggggtc tatcagctct
      481 actgaacgtt catttttatg cgataaaact atccgctcag aagtggaatc ccgtaaaaca
      541 aattccattt ttaaataagc tcggggctcg gcaccttcta tcttttcaat attatacact
      601 gtcgtttcca gaaaatagtg aggttcttta tccaaaattt ccccgcggga tacagttcca
      661 aaaatattgt atgctttcac acgctggaca actaagttgg gaatagcatc tcgtagcctt
      721 gttgcccaaa catcagtctg caggaatttt actttataaa aattctcttt taccactatc
      781 tgattacggt cataagttct gggcaaaact gtatccagca cttccagatt ttttcctgta
      841 ttattagctc tgcgtaattc aggtttttca cttcctggtt gatattccaa aacatagtag
      901 tttacaggtg ttttttccac cttttgcaag cagcctgaaa gcataattac caaagctaaa
      961 catcctataa tgaagcacat actctttatt ttatgcataa taactcctaa tttccgcgta
     1021 gcaagatagc aggcgtatcg gcaatctgac gagaaaattc attcagattt tcactcgtct
     1081 cggttagggt gcttaaaatt tccagtaagt ctccctgacc acgcgttaca actctattca
     1141 agttcgcaac caaatttcct gttttatgaa ttgtagtgga tagctcactt accatttcat
     1201 taatattagc tctagaaagt tctccgcaca gagtccccag gttagtcatt aaactgtcta
     1261 ctcctggagt ataaagaata gcattcacct ggtctaatgt cttattaata ttattactga
     1321 tcgttaatat ttcgcgggta gtttccaaaa tcatttgatc gctatgttca cctactgtag
     1381 taacttttaa attagcctcg cgaatcaaag cagagccatc ttcagttaac cttgccaagc
     1441 tatattctag ttgagaagtt aattcctcca actgtttatt tacattacta gtaacgctaa
     1501 ttaaacttgt ctgcgaggtt tccgaaacac tttgaatatt gcttgtagcg ctattagtga
     1561 tagcatcaag attcttagtg agattatcgg ttactgcact taaatctcta cttagttcct
     1621 ctgttaaatc cgctgtatta tttgctattc tactaatgct ttgcatcgta gtagccagat
     1681 tttcgtgagt atctttcaaa atcaaacttg tttgctcgag gatcttagaa atattctgcc
     1741 tgttttcctc tccagtaatt aagtttaggt tggcagctat catatcgatt ttatccacaa
     1801 ttgattttgc ccgttcactg atatcttcca acattgtagt tccaggcttt atataacttt
     1861 taggtttcaa cagtttagct tcattagttc cgccccgaat ttccacagat ttcagcccag
     1921 ttattcccga taacagtaaa acggcttccg tatcttcttt tataggggtt ccccggttaa
     1981 cacttataga gacaattatt ttagttacat ccttaggatt gattttaata tcttctactc
     2041 taccgatttt aataccgcgg taattaaccg aactgccaac ttgcaatcca ctaacagaat
     2101 agtcctcaaa ttcaatataa tatatatctt tcttttctac taatctgcta ccagctacaa
     2161 ttgccgcaaa aattataatc agaattgccc cgatagttaa aaaaacccct agtcgtattt
     2221 tagcagcctt ggaaaccata ttcatcctcc tgctttgtct tgagggaaat ttctttgcat
     2281 atcaaaattc acttctccgg taagcaaaca attttgcaca aataccggat tactggcttt
     2341 tagagttgtt ttgccctcac aaataactcc ttctccaaaa cttacatccc cgtaaatttt
     2401 caattcctta caaccgctta aacaagggat acctttgcta aaacgctttt gcatatcatc
     2461 aattttccca taatagattt catccaattc aattaccggt ggtttttcca gaccacgctt
     2521 caacactacc tgatacatat cgttcagatc ataagcatcc gaccaaagag ctaaaagatc
     2581 atttgttttt tttacaggtg caaatcgttc cctgggaaca attaatgcct tggaattgtt
     2641 aaatatacta attgccgaac ccatagctgt ttccaattga taaacaggag ttccttccac
     2701 tatcttagga ttaacaatta acggtaacag cagcaaacct tcattagcaa ttatatacca
     2761 ttctaaagct cttaaatcta tccacaagtt attagtattg aaatatttgt aatattcgat
     2821 atcttgaaag cggggcaaat cattatcgga gcattgag
//
LOCUS       DAORHB010000163         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_41538_length_2831_cov_4.752522, whole genome shotgun sequence.
ACCESSION   DAORHB010000163 DAORHB010000000
VERSION     DAORHB010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41538_length_2831_cov_4.752522"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKM80_05110"
     CDS             <1..192
                     /locus_tag="PKM80_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HNV93325.1"
                     /translation="SIEAIIPGYYDKYNSTMRGNYDKYIALIKDRFQQRKDFLQSSDL
                     TEKPDIQTKLIQSLQVNAD"
     gene            complement(886..2007)
                     /gene="mnmA"
                     /locus_tag="PKM80_05115"
     CDS             complement(886..2007)
                     /gene="mnmA"
                     /locus_tag="PKM80_05115"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNV93326.1"
                     /translation="MQNKICVAVGISGGIDSAMTAYLLKESGYRIIGITMQTWDKNIA
                     MEKVLKSGCFGPAQEESIRASAILCQKMGIEHYVIPLQEEFQQKVINYFCQTYLQGET
                     PNPCLVCNAHLKFALLPQKAKLMGLEFDYFATGHYAKVDYNKELQRYQIKRARDKNKD
                     QSYFLAFLSQEQLAGTLFPLGDYTKPEIKKMAAEIGFGELVTRQESQDFLQSSDLEVL
                     FKAEEYQQGEIVDINGKIMGTHKGIINYTIGQRRNLGVSGLPEPYYVLKIDAINNRIV
                     LGTQQYLYKNRCKATGMNWVSISAPEKQFNAKAKIRQQHTPADCIVTPIDDRNIEVVF
                     AKPQLSITPGQGLVLYDEDLLLGGGFIALSNNDDAEHNA"
     gene            complement(2546..>2831)
                     /locus_tag="PKM80_05120"
     CDS             complement(2546..>2831)
                     /locus_tag="PKM80_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93327.1"
                     /translation="GAVISDPQPEPTKVNHLLQSKGSDYVLAYTTAYTTESRRLNLVY
                     SAVGWGINTFIASVYYFHIVHDIDSDFDDWFKNQRKIFISVPLKNPAHPY"
BASE COUNT          887 a          550 c          465 g          929 t
ORIGIN      
        1 tccatcgaag ccattatacc cggttattat gataaatata attccactat gcgcggtaac
       61 tatgataagt acattgcttt gattaaagat agattccaac aaagaaagga ctttttgcaa
      121 agtagcgatt taaccgaaaa accggatatc cagacaaaat tgatccagtc cttgcaagta
      181 aacgctgatt agaacttttt ggctggcata cttattccct gcataaaaaa gggttgccca
      241 aattctgatt ggccaaatgc caaggatgtc attcttttgc agcccgagta actatgcagt
      301 aaagagtaac ttgccctaaa aaatgtttca tagatagcgg gattaatttt ccgctatctt
      361 ttttatcctt ccttaattag ctatcatgga gctatggtgc tgatcagagg tattttcccg
      421 agaaacacca ccgcatctct accataagta atcaagcaag gattataggt ttacagcttt
      481 agaaacaagg attttaggat tagttcacgc agattacgca gatgacgcag atttgatttt
      541 agaaacaggg attataggtt tacagcttta gaaacaagga ttttaggatt ataggattta
      601 gaggattttt aaagttccgt taggaacgat acatactaac gacgggtttt aacccgttga
      661 aatataaaac aaataattta gtcccatcgg gacgacagat tttaacgacg ggttttaacc
      721 cggcgaaata taaaacaaat aatttagtcc catcgggacg acagatttta gcgacgggtt
      781 tttaacccgg cgtaaaaata acggaaggtt gaagtcctag ccaaccacaa taaacaaact
      841 atcaagctat taaaaaatct caaccttcta aactactaaa accctctaag cattatgttc
      901 tgcatcatca ttattggaaa gtgcaataaa tccacccccc aaaagtaaat cctcatcata
      961 taaaaccaag ccttgtcctg gagtaatgga aagttgcggt ttggcaaaaa ccacttctat
     1021 atttctatca tctatcggtg ttacaataca atcggcggga gtatgttgct gtcttatttt
     1081 ggctttggcg ttaaattgtt tttccggtgc agaaatggaa acccaattca tacctgtggc
     1141 tttacaccga tttttgtaga gatattgctg agttcccaaa acaattctat tatttatggc
     1201 atcaattttc agaacataat agggttcagg caaaccgctt acgcccaaat ttcttctctg
     1261 gccgatagtg taattgatta tgcctttgtg cgttcccata attttcccat ttatatctac
     1321 aatttctccc tgctgatatt cttccgcttt aaagagcact tctaaatctg aactctgtaa
     1381 aaaatcctga ctttcctgac gagttacgag ttcgccaaaa cctatttcag cagccatttt
     1441 ttttatttcc ggctttgtgt aatctcctaa cggaaaaaga gttcctgcca attgctcctg
     1501 tgacaaaaaa gccagaaaat aggactgatc tttattttta tctcttgctc tttttatttg
     1561 gtagcgttgc aattctttat tataatctac tttagcatag tggcctgtgg caaaatagtc
     1621 aaattccaac cccattaact tagctttttg gggtaataag gcaaacttaa gatgggcatt
     1681 acaaaccaag caaggattag gtgtttctcc ctgtaaatag gtctggcaaa aatagttaat
     1741 cactttctgt tgaaattctt cctgcaaagg aataacataa tgctctatcc ccattttttg
     1801 gcataaaata gcagaggcac gaatactttc ttcttgagcg ggaccaaaac aaccactttt
     1861 taacactttc tccattgcta tgtttttatc ccaggtctgc atcgtgatac caattattct
     1921 ataaccgctt tcttttaata aataggcagt catagctgaa tcaatccctc cactaattcc
     1981 gactgctaca cagattttat tttgcatatt cttcactaac ctcagagttt attttcatac
     2041 agttctatct gctttacact tttattaatt cgtcaaagat aacatccaac tgcttatcaa
     2101 ttatgttctt ttgcctaata ttattacttc gtgcctcaaa atttatttat gctgatagat
     2161 aagataataa caatattccg gttattgtca accaatattt catttatcct tatccccaat
     2221 gccctcttct ggtaaatcta tatagtattt cctcatatct atatatttag aagggtaaaa
     2281 atgaagatct aatctgcacc gcataatcct atctgtcact ttttaaaatt cctttaaaag
     2341 ctctatgtct tttttgctac tttacgctgt ataatgagaa caacttgggt acatcagaca
     2401 tctttgcccg taggttggcg aagtggattg ctgataataa ctgattacga ttgagtaact
     2461 tgtgaatgaa gaagagaatt ttagatttga ttcagggctc ttcatcatat tctcagcatt
     2521 taaaattgta ttattgaagt ttaaatcaat aaggatgagc aggattctta agtggcacgg
     2581 aaataaaaat ctttctctga ttcttaaacc aatcatcaaa atctgaatca atgtcatgaa
     2641 ctatatggaa ataataaaca ctagcaatga aagtatttat tccccaacct actgcagaat
     2701 atactaaatt aagcctacga gattctgtag tgtatgcagt agtatatgct agaacataat
     2761 cactgccttt ggactgaagt aaatgattaa ctttcgttgg ttctggttga ggatcagata
     2821 taacagctcc t
//
LOCUS       DAORHB010000164         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_42192_length_2803_cov_6.385371, whole genome shotgun sequence.
ACCESSION   DAORHB010000164 DAORHB010000000
VERSION     DAORHB010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42192_length_2803_cov_6.385371"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKM80_05125"
     CDS             <1..1039
                     /locus_tag="PKM80_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNV93328.1"
                     /translation="KNDFNSILTCFAPIAKERNKQTFLPQDKQIDIIIGTDCISEGQN
                     LQDCDYVINYDIHWNPVRIIQRFGRIDRINGPNAQIQMHNFWPTKDLDKYIDLKYRVE
                     TRMALVDLTATGADNILSEEDIDNLVANEKKFRTKQLKRLQEEVIDLEDLEDSISLTD
                     FTLDDFRIDLLNYLKANEVELSNAPLGLYAITPSPHNKLWLKTNNLSEEQKEIIRPGI
                     IFCFQHFADGSEYNKLNPLQPYFLVYIRDDGTVRYHFTNVKQILEIYRLLTIGRKEAI
                     KKLCDLFDDETNNGQNMDKYNNLLIKAIDDIKDTLNKRSYKQLQGDRNAIIPKKPQEQ
                     SFELITWLIIK"
     gene            1050..>2803
                     /locus_tag="PKM80_05130"
     CDS             1050..>2803
                     /locus_tag="PKM80_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93329.1"
                     /translation="MDKESIKEALSLFQKQDTYSAALKFWNTLGYLSDRQPEQHSFSY
                     NDFAQFCGNNINAFKAKKEDWDQFHLLFQITDEEMRNHFNSYYQEVIPGNIKQEFQAS
                     NIKSYLFAALKLKGNTYNRTQLADIARQINKQYAIPLIILFHYNKFITIAVVSRRQNL
                     KDTQRDVLEKVTMIKDIKLTEPHRAHIDILDDLSIEKLSEKYTLNSFDALHNAWSATL
                     DIEELNKRFYKELSNWYFWAIKKVVFPAGEESNEEVRNSIGLIRLLTRLIFVWFMKEK
                     GLIPDALFDPQKIKTIIKFEDFNDSSYYKAILQNLFFATLNTEMNKDKENSRKFRREI
                     KSKMNPDFNVHTLFRYESLFHHPEQVIDEYFANIPFLNGGLFECLDTEITQNNNNNYI
                     RIDGFSDRPDNVLKVPDELFFSDKEQDIDLNEFYGTTNKRYQVCGLLNILNSYKFTVT
                     ENTPIEEEVALDPELLGRVFENLLASYNPETKTTARHETGSFYTPREIVDYMVDESLI
                     AYLLNELPHSTKAEAEDSELKLRLLLYYTDEDHLFNPEEVDKLIYAIDNLKIIDPACG
                     SGAFLMGLLLKIVYILHK"
BASE COUNT          984 a          472 c          487 g          860 t
ORIGIN      
        1 taaaaatgat tttaattcca ttttaacctg ctttgctccc attgccaaag aacggaataa
       61 acaaactttc ctgcctcaag ataaacaaat tgatattatt ataggcaccg attgtatctc
      121 tgaaggacag aatttacagg actgtgacta tgttataaat tatgatattc actggaaccc
      181 tgtgcgtata attcaacgct ttggaagaat agacagaata aatggtccca atgcccaaat
      241 ccaaatgcat aatttctggc ccaccaaaga cctggataag tatatagacc ttaaatatag
      301 agtagaaaca agaatggctt tagttgatct gactgcaacg ggtgctgata atatcctcag
      361 cgaagaagat atagacaatc tggttgccaa cgaaaagaag ttccgaacca aacaactaaa
      421 gcgtttacag gaagaagtta ttgacctgga ggatttggaa gatagcatta gcttaaccga
      481 ttttactttg gatgacttta gaatagacct gttgaactat ttaaaagcta acgaagttga
      541 attaagcaat gcccctttgg gtttatatgc tattactcct tcccctcaca ataagttgtg
      601 gcttaaaacc aataatttat cagaagaaca aaaagaaatt atccgacccg gtattatttt
      661 ttgtttccag cattttgccg atggctcgga atataataaa ttaaatcctt tgcaaccata
      721 ttttcttgtt tacatcagag acgatggaac tgtccgttat cattttacca atgtgaaaca
      781 aattctggag atttacagat tgctgaccat aggaagaaaa gaagctataa aaaagctttg
      841 cgatctcttt gatgatgaaa caaacaatgg tcaaaatatg gataaataca ataatcttct
      901 tataaaagca attgatgaca tcaaggatac attgaacaaa agatcatata aacaattgca
      961 aggagataga aatgccatca ttcctaaaaa accccaagaa cagagttttg aactaatcac
     1021 ttggctgatt atcaaataaa ggaatataaa tggataaaga aagtattaaa gaagccctgt
     1081 ccctttttca aaagcaggat acttattccg ctgctctgaa gttctggaat actttaggtt
     1141 atcttagtga ccgtcaaccg gagcaacata gtttcagtta caatgatttt gctcaatttt
     1201 gtggaaataa tatcaacgcc tttaaagcta aaaaagagga ttgggatcaa tttcatctgc
     1261 tgtttcagat tacggatgaa gaaatgagaa accacttcaa tagttattat caagaagtta
     1321 ttcccgggaa tataaaacag gaatttcaag catccaatat caaatcatat ctttttgctg
     1381 ctcttaaact taaaggtaat acttataacc gcactcagct tgccgatata gccaggcaaa
     1441 ttaataaaca atatgctatt cctttaatta ttctctttca ttacaataag ttcatcacta
     1501 tcgcggttgt ttccagaagg caaaacttga aagacacgca aagagatgtt ctggaaaaag
     1561 ttactatgat aaaggatatc aaattaactg aaccccatag agcacatatt gatattttag
     1621 atgacctgag tatagaaaaa ctctccgaaa agtataccct caatagtttt gatgccttgc
     1681 ataatgcctg gtccgctact ttggatattg aagaattaaa taaaagattt tataaggaac
     1741 tctccaattg gtatttttgg gcaattaaga aagttgtttt tcctgcaggg gaagaatcaa
     1801 atgaggaagt aagaaattcc attggtctaa tcagattgct aactcgtctt atttttgttt
     1861 ggtttatgaa agaaaaaggg ctaatacccg atgctctttt tgatccccaa aaaataaaaa
     1921 ccattatcaa gtttgaggat tttaacgatt cttcctatta taaagctatt ctgcaaaacc
     1981 tctttttcgc taccctgaat acggaaatga ataaagataa ggaaaattcc cgtaaatttc
     2041 gcagagagat taaaagtaaa atgaatcccg atttcaatgt gcatactctg tttcgttatg
     2101 agtccttgtt tcatcatcca gaacaagtta tagatgaata ttttgccaat attccctttc
     2161 taaatggagg tctgtttgaa tgtctggata ctgaaattac ccaaaacaat aataataatt
     2221 acattcgcat tgatggtttt agtgaccgac cggataatgt attaaaagtt ccggatgaat
     2281 tgttttttag tgataaggag caagatattg acctaaacga attttatgga actacaaata
     2341 aaagatatca agtgtgcggt ttacttaata ttttaaacag ttataaattc accgtaacag
     2401 agaatactcc catagaagaa gaagttgctc ttgatccaga attactgggt agggtttttg
     2461 aaaatctgct tgcctcttat aatcctgaaa caaaaacaac tgccagacac gaaaccggtt
     2521 cattttatac acctcgtgaa attgtggatt atatggtgga tgaatcatta atagcttatt
     2581 tgctgaatga attgcctcat agcactaaag cagaagcaga agatagtgaa ctaaagctaa
     2641 ggttgcttct ctattataca gatgaagatc atctctttaa tcctgaggaa gtggataagc
     2701 tgatttatgc cattgataac cttaaaatta ttgatcctgc ttgtggctca ggagcttttt
     2761 taatgggtct gctgttaaaa attgtctata tcctgcataa gtt
//
LOCUS       DAORHB010000165         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_42513_length_2790_cov_4.196709, whole genome shotgun sequence.
ACCESSION   DAORHB010000165 DAORHB010000000
VERSION     DAORHB010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42513_length_2790_cov_4.196709"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKM80_05135"
     CDS             complement(<1..365)
                     /locus_tag="PKM80_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNV93330.1"
                     /translation="MQNSDIDIVMEQLKEHFHSVKTPVVDLIKIKTDDPFKVLVATIL
                     SARTKDETTAKVAEKLFQKVNKAEDFDKLTYEELDKIITPVGFHRVKAKHLKQLPKVL
                     KEKFNGKIPEEIDELMVIPG"
     gene            544..1239
                     /locus_tag="PKM80_05140"
     CDS             544..1239
                     /locus_tag="PKM80_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNV93331.1"
                     /translation="MCGRFAQVITHKELQKLEAELKIKKSSDQLEFNYNVAPTATVAA
                     VVSQADFRYLGFFRWGLIPSWRKEIPEQALINIRSETILEKPSFKASFLRRRCLIPVN
                     GFYEWRKTDKQPFFIKAKDSELLYLAGIYDSWHSPDGSYIPSLGIITTSANPYLQALH
                     NRMPLLLSPAVYDLWLDPNLNNPQELQPLLSPSSEIELEMYPVDKRINSPQNNDADCL
                     KPIKLSTAKDNYL"
     gene            1248..2330
                     /locus_tag="PKM80_05145"
     CDS             1248..2330
                     /locus_tag="PKM80_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV93332.1"
                     /translation="MERWKQHILVVDDDVAILDCIRMSIKKDIPCQITTTSDSLEALE
                     LLKNNVYNVVIADINMPNLSGFQLLEFISTISPNTPVILITGSTEPEKLRIAIQLGAH
                     DILRKPFELSDLHMAINNALQKNALLLQNEVYRLNLEHIVQQRTQELYAAKSKLEKNY
                     LNTIHAMVNAMEANDIYIRGHSERVTVLSIMIGKLLNLSAKELNLLRVGALLHDLGKI
                     GIYDTVLNKKDNLTPSEFSLIKQHPVIGAQIVRPIGLPEAVYNIILQHHEWFGGGGYP
                     YGLTQENISLLARIVTIADSYDAMTSKRVYRHNLEPNAARTEIINNIGFQFDPDIGLL
                     FFEKFCQITEPLSDTPAIQNLLSELI"
     gene            2361..>2790
                     /locus_tag="PKM80_05150"
     CDS             2361..>2790
                     /locus_tag="PKM80_05150"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNV93333.1"
                     /translation="MVQVISATEAAAMVKPGSRIAIGGFLAVGAPETIIDAMVENENN
                     NLHMIVIASDWENRGVGKLIVNRMVTSAQVSHMGTNKTIQAQMNAREIEIELIPQGTL
                     MERVRAFGAGLGGILTPTGLGTIVEEGKQIISIEGVDYLLE"
BASE COUNT          873 a          529 c          512 g          876 t
ORIGIN      
        1 cctggaatta ccattagctc atcaatttcc tcggggatct ttccgttaaa tttctccttc
       61 agcacttttg gtaactgctt taaatgttta gctttaactc tatgaaatcc aacgggagta
      121 attattttat ccagttcttc ataggtaagt ttatcaaaat cctctgcttt attcaccttt
      181 tgaaacagct tttctgcaac tttggcagta gtttcatctt ttgttctcgc gctcaaaatt
      241 gttgccacca acactttaaa tgggtcatca gttttaattt tgatcagatc tacaaccggt
      301 gttttaacac tgtggaaatg ctcttttagc tgttccatca cgatatctat atcgctattt
      361 tgcataatat ctccattcat attagtcttt ttttgtggac gctgcctatt caatatttaa
      421 aggttctttg tatacaggaa gacaaaaaaa gaggatttat ctccccaaat atctaagagg
      481 taaaaatatt tattgcctaa aatatggctt ttataaatct tgtcaacttg aaaggagata
      541 taaatgtgtg gacgctttgc ccaggttatc actcataaag aactacagaa attggaagct
      601 gaactgaaaa ttaaaaagag ttcagatcag cttgaattta attataatgt agctcccacg
      661 gctacagttg ctgctgttgt ttcccaagca gattttcgat atctgggttt tttccgttgg
      721 ggtttaattc cttcctggcg aaaagaaatt cctgagcaag cacttatcaa tatccgcagc
      781 gaaacaatat tagaaaagcc aagttttaag gcatcttttt taagaaggcg ttgtcttatt
      841 cccgtcaatg gtttttatga atggcgaaaa accgataagc aacccttttt tattaaagca
      901 aaagatagtg aattacttta tctggcagga atttatgaca gctggcatag tccggatggt
      961 agttatatac cttctttagg aataattacc acttctgcca atccttatct tcaagctttg
     1021 cataatcgaa tgcctctatt attaagcccg gctgtttatg atctttggct tgatcctaac
     1081 ctaaataacc cccaggagtt acagccattg cttagcccca gttctgaaat cgaactggaa
     1141 atgtatccag ttgataaaag aattaatagt ccccaaaata acgatgcgga ttgcttaaag
     1201 ccaattaagc tatctaccgc taaagataat tatttataga gtaatatatg gagcgctgga
     1261 aacaacatat tttagtagta gatgacgatg tagctattct ggattgcatc cggatgagca
     1321 taaaaaaaga tattccttgc caaataacta ctaccagcga ttctctggaa gctttggaac
     1381 tgctaaaaaa taatgtttat aatgttgtta tcgctgatat taatatgccc aatctttctg
     1441 gatttcagct tttagaattt atcagcacaa tcagcccgaa cactcccgtg attcttatta
     1501 ctggctctac agaacctgaa aaattgagaa ttgctatcca attaggagca cacgatattc
     1561 tgcgtaagcc atttgaatta tccgatttgc atatggctat taataatgcg cttcaaaaaa
     1621 atgctcttct cttgcaaaat gaagtatacc gtcttaatct tgaacatata gttcagcaac
     1681 gcactcaaga actttatgcc gcaaaaagta agctggaaaa gaattacctt aataccattc
     1741 atgcaatggt taatgccatg gaagcaaatg atatttatat tcggggtcat tccgaacgcg
     1801 ttactgtgct aagtattatg atcggcaaac ttttaaattt gagtgccaaa gaattaaatc
     1861 tattacgagt gggagctcta ttacacgatt taggcaaaat cggcatctat gataccgtcc
     1921 tgaataaaaa ggataactta acccctagcg aattttctct cataaaacag catcctgtta
     1981 tcggagcaca aattgttcgc cccattggtt taccggaagc tgtgtataat attatattac
     2041 agcatcatga atggtttgga gggggtggtt atccttacgg tcttacccaa gaaaatattt
     2101 ctcttttagc cagaattgtc actattgctg attcctatga tgcgatgacc agtaaacgag
     2161 tataccgtca taatcttgaa ccaaatgcag caaggacaga gataattaat aatataggtt
     2221 ttcaatttga cccagacatc ggtttactat tctttgaaaa gttttgccag attacagagc
     2281 cgttatcgga tacaccagct atccaaaatc tgctctctga attaatataa acagcatatt
     2341 aaaatataca aggagacaaa atggttcaag ttattagtgc cacagaggca gctgctatgg
     2401 ttaaaccagg aagccggatt gccatcggcg gttttttagc tgtgggagct ccagaaacta
     2461 ttatcgatgc aatggtggaa aatgaaaata ataacctcca tatgatcgtt atcgcttccg
     2521 attgggaaaa ccgaggtgta ggtaaactca tcgtaaacag gatggttaca agtgctcaag
     2581 tttcacatat gggaaccaat aaaactatcc aagcccagat gaatgccaga gaaatagaaa
     2641 ttgaacttat cccccaagga acacttatgg aacgcgttcg cgcttttgga gctggtttag
     2701 gaggtatttt aacccccact ggattgggca caattgtgga agaaggaaag cagattattt
     2761 ctattgaggg agtagattat cttctcgaac
//
LOCUS       DAORHB010000166         2716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_44272_length_2716_cov_3.589628, whole genome shotgun sequence.
ACCESSION   DAORHB010000166 DAORHB010000000
VERSION     DAORHB010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44272_length_2716_cov_3.589628"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1772
                     /gene="purL"
                     /locus_tag="PKM80_05155"
     CDS             <1..1772
                     /gene="purL"
                     /locus_tag="PKM80_05155"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNV93334.1"
                     /translation="ERITSILNREPTIVELGIFSVMWSEHASYKNSVLLLKTLPKEGS
                     SLLAKAGEENAGLIDIGHGLAISFKIESHNHPSALEPYHGAATGVGGILRDIFTMGAR
                     PIAALNSLRFGNPGNPKVQHLLKEVVRGIADYGNCFGVPTVGGEVYFDDSYEGNPLVN
                     AMAVGIVKHNKIARSAASGVGNAVLIVGAKTGRDGIHGATFASVDLSEKSEEKRTAVQ
                     VGDPFLEKLLLEATLEAIDEELVVAIQDMGAAGITCSTSEMAGKGNVGIEIDVALVPQ
                     RETDMTPYEIMLSESQERMLVIVKPENLEQIKSIFTKWELDAVQIGKVTDDKMLRVFY
                     GEEKVAEIPAETLILGGSAPVYKRESKYPEYLDALHNYDIENLPEPDTYQEVLLKLIA
                     SSNISSRHQIYQQYDHMVQIGTVVEPGSDAAVVRIPDTDLAIGLSTDCNGRYCYLDPY
                     LGALLAVAESARNVACSGAKPIAITNCLNFGNPYKPEVYYCFKESIRGMSDACKALGT
                     PVTGGNVSFYNENPEGAIYPTPVIGMLGLMTDYRKATTQYFKHENDIIVLLGSLKASF
                     GGSEYLKIQHNIIAGKPPIVDLE"
     gene            1797..2147
                     /locus_tag="PKM80_05160"
     CDS             1797..2147
                     /locus_tag="PKM80_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNV93335.1"
                     /translation="MELIDKGLVNSAHDISEGGLLIAVLESCFNPEQLFGAKLSFDLE
                     LNPAKIYFGEAAGRILISAAKDKYNSIKEAAMKHGIEYHKIGIVSANSSFIVNNHINE
                     DMAILHSAWKQGII"
     gene            2169..2687
                     /locus_tag="PKM80_05165"
     CDS             2169..2687
                     /locus_tag="PKM80_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93336.1"
                     /translation="MNFVFSNFFWGLFFILLGLSVFLKAFNIHLPLVRVFIAIIIIMI
                     GIKLLFGLKSHHYHKPKSYSKENSIYYSSSRDEYNIIFSSGIIDLSDLPENAKDFEIT
                     VVFGSGTVILPDNIAVNINPTSVFGSTILPPKNPVTQDKPVEIESTVVFGRLEYKYAK
                     AEQTTEQTTEPQ"
BASE COUNT          870 a          448 c          566 g          832 t
ORIGIN      
        1 atgaacgaat tacaagtatc ctgaaccgtg aaccgactat tgtggaactg ggtattttca
       61 gtgtgatgtg gagtgaacat gccagctata aaaactccgt tttattgctt aaaacccttc
      121 ccaaggaagg ttcttctttg ctggcaaaag caggagaaga aaatgccgga ttgattgata
      181 tcggacacgg attggcaatt tctttcaaga ttgaaagtca taatcatcct tcagctttag
      241 aaccctatca tggggctgca actggagttg gaggtatttt aagagatatt tttacaatgg
      301 gagcgcgtcc aattgctgcc ctaaattctc tgcgttttgg caatccggga aatcctaagg
      361 ttcagcattt gctaaaggaa gttgttcgtg gcatagcgga ttacggaaat tgtttcggag
      421 ttccaactgt gggaggagaa gtctattttg atgatagtta tgaaggcaat cctttagtta
      481 atgcaatggc cgtaggaata gtaaaacata ataaaattgc ccgttcagca gcctccggag
      541 taggaaatgc agttcttatt gtgggtgcca aaacaggacg cgatggaatc catggagcta
      601 cttttgcctc tgtagattta agtgaaaaat cagaagaaaa aagaactgct gtccaggtgg
      661 gtgacccatt tctggaaaaa ctattactgg aagcaacttt agaagctatc gatgaggagc
      721 ttgtagttgc cattcaagat atgggtgctg caggcatcac ttgttctact tctgaaatgg
      781 ctggtaaggg taatgtaggt attgaaattg atgttgctct cgttccacaa agagaaacgg
      841 atatgactcc ctatgaaatt atgctttctg aatcacagga aaggatgctg gtaattgtaa
      901 aaccggaaaa tttggaacaa atcaagtcta tttttacgaa atgggaactt gatgctgttc
      961 agatcggaaa agttactgat gataaaatgc taagagtttt ctatggggaa gaaaaagtag
     1021 cggaaattcc ggctgaaaca cttattttag gtggttcagc accagtttat aaaagagaaa
     1081 gtaaatatcc tgaatacctt gatgcactgc acaattatga tatagagaac cttcctgaac
     1141 ctgatactta tcaggaagtt ctattaaaac ttattgccag ctccaatatt tccagtcggc
     1201 atcagattta tcagcaatat gatcatatgg ttcagattgg aaccgttgtc gagcctggtt
     1261 cagatgcagc tgttgttaga attccagata cagatttagc aattggactt agcaccgatt
     1321 gtaatggtcg atattgttat ttagatcctt atctgggagc tttattagct gtagcagaat
     1381 cagcaagaaa tgtcgcttgc agtggagcaa aaccaatagc tattaccaat tgcctcaatt
     1441 tcggcaatcc atataaacca gaagtatact attgttttaa agaatccatt cggggaatga
     1501 gtgatgcctg caaagcttta ggaactccag ttactggagg taatgtatct ttttacaatg
     1561 agaatccgga aggggcaatt tatcctactc ctgttattgg aatgttaggg ttaatgacag
     1621 attataggaa agcaacaacg cagtacttta aacacgaaaa cgacataatt gttcttttgg
     1681 gctctttaaa agctagcttc ggaggtagtg aatacctaaa aatacagcat aatataattg
     1741 ctggaaagcc gcctattgta gatttggaat aagaattaca tttacaggat ttaattttgg
     1801 aactaattga taaaggatta gttaatagtg ctcatgatat atccgaaggg ggcttgctta
     1861 ttgctgtttt agaatcttgt tttaatcctg agcagttgtt tggagctaag ctatcttttg
     1921 atcttgagct gaatcctgct aaaatatatt ttggagaagc agctggcaga attcttatta
     1981 gtgcagcgaa agataagtac aattctatta aagaagcagc tatgaaacac ggaattgaat
     2041 atcacaaaat aggaattgtt tctgctaatt caagttttat cgttaataat catattaacg
     2101 aggatatggc gatcctacac tctgcctgga agcagggaat aatataaaaa aagggaaagg
     2161 aaatgaaaat gaattttgtt tttagcaatt tcttttgggg attattcttt attctcttag
     2221 gactatctgt atttttaaag gcgttcaata tccatctacc tttagtgaga gtttttattg
     2281 ccattatcat tattatgatt ggtatcaaac tgctttttgg cttaaaatcg catcattatc
     2341 ataagccaaa atcttattct aaagaaaatt ccatttacta tagcagttcc agagacgaat
     2401 ataatataat cttttcttcc gggattatag acttaagcga tctaccggaa aatgccaaag
     2461 atttcgagat tactgtagta tttggttccg gaacagtaat tctacctgat aacattgccg
     2521 ttaatatcaa tcctacttca gtttttggat caacaatttt acctcccaaa aatcctgtaa
     2581 cgcaggataa acctgttgag attgaatcca cggtagtttt tggacgcttg gaatataaat
     2641 atgccaaagc tgaacaaaca acagaacaaa caactgagcc ccaataagga acatacaaat
     2701 ataaaactga ctttat
//
LOCUS       DAORHB010000167         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_44536_length_2706_cov_3.821954, whole genome shotgun sequence.
ACCESSION   DAORHB010000167 DAORHB010000000
VERSION     DAORHB010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44536_length_2706_cov_3.821954"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1120
                     /locus_tag="PKM80_05170"
     CDS             323..1120
                     /locus_tag="PKM80_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93337.1"
                     /translation="MRKQVWLIIPLIILIVIGGCKKKAHTLPDKETANAITAYAPIPS
                     YKEVFRFLDKYQAKEISAAIPDELYKTKQEDVRNAFSLGVISVDAILSAKGQNKKRLN
                     QYYSQMLDLTSVLGLDEEVNQISPNLKKHINEGQWLELETAIDAHKKKVEDIFWEKEI
                     FDYYTLLQLGSWIEATNRTAWLIQQNYSVEHTRALHQKEAFQNIIANMNNIDSEAIVN
                     QPEFQEVMGLVQQLKAIIDADYEKTYTSEQLDQIISLTNQIKETFQK"
     gene            1192..1641
                     /locus_tag="PKM80_05175"
     CDS             1192..1641
                     /locus_tag="PKM80_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93338.1"
                     /translation="MKKILLVLLMGLLLSFLFAKSSFFWVEDVPWDDGKALIIKANVP
                     ADTVYLQKALEDGSWQTLYAGKGINLEYTDSELNPDLEYRYRLLVPRPTFGDSISFKA
                     IAKPIEQWFYLHKLSYLLILLAVSFAIVYYVQKARKERIIIFAGSTA"
     gene            1818..2291
                     /locus_tag="PKM80_05180"
     CDS             1818..2291
                     /locus_tag="PKM80_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93339.1"
                     /translation="MVLSASREVVKEAYIKAGKPDAYNQDRIFYLTDDQFGYVAGIDG
                     IMVREQPAANFFLGSFYAESLILAETGFSTGAIQTAGTAQASQLPFFVVSCDYTLIGE
                     ELFAASAYLSRDPQQLGSLKGHDFGKLLVILLIVAGVIAEMSNWHWFKAFFEGAQ"
     gene            2288..>2706
                     /locus_tag="PKM80_05185"
     CDS             2288..>2706
                     /locus_tag="PKM80_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93340.1"
                     /translation="MKYKVPLIFAFVCGILVVISEFIPHRPFNMLITTLQDWFMIISG
                     FAIILGQISLFQANIKKIRGKNPDWPFYLAGLISFSIMLIVGLFWGTQEYRGILGHGD
                     KIQAAIGMKPFDYLFANAFMPLSSTMFSLLAFFIASAA"
BASE COUNT          872 a          439 c          519 g          876 t
ORIGIN      
        1 catagcgagc tccctgaaaa gaaataatta actatctctt ctattaagga gattttactg
       61 agattggcag aatgaaaata atgctaattt cgtttggact taccaagatt gtttaacaag
      121 gaatatctaa cactgtcaaa aagatagaga aacaaagggt tagaattttc tctaacaatt
      181 aggaaacagc tggaaagaag aaaagcagtt ttgtacttga cagttctctc tggtcaaaaa
      241 ttaattaaaa aaagttgaat aactcactaa gttgtataca taactttttg gaaaatgatg
      301 atttagagat aaggagtaaa caatgcgaaa acaagtctgg ttaattattc ccctcattat
      361 tctgattgtg ataggcggtt gtaaaaagaa agctcatact cttcctgaca aagaaaccgc
      421 aaatgcaata acagcgtatg ctcccatacc ttcttataaa gaagtttttc gtttcttaga
      481 taaatatcaa gcgaaagaaa tatctgcagc tattcctgac gaactgtata aaacaaaaca
      541 ggaagatgtt cgcaatgcat tttctttggg agtaatttct gtggatgcaa tactatctgc
      601 taaaggtcaa aataaaaaac gattaaatca atactattct cagatgttgg atttaacatc
      661 tgttcttggt ttagatgagg aagtaaatca aattagtcct aatttgaaaa aacatattaa
      721 cgaaggacag tggttagaat tggaaactgc aatagatgct cataaaaaga aggttgaaga
      781 tatattctgg gaaaaagaga tctttgatta ctacacttta ctgcaactcg gaagctggat
      841 cgaagcaacg aatcgtacag cttggctaat acaacaaaat tactctgtgg agcatactag
      901 ggctttacac caaaaagaag cttttcaaaa tataattgcc aatatgaata atatcgactc
      961 agaagctatt gttaatcaac ctgaatttca agaagttatg ggtttagtac aacagctaaa
     1021 agcaattatc gatgccgatt atgaaaagac ctacacttct gaacagctgg atcagattat
     1081 ttctctaact aatcagatta aagagacatt tcaaaagtga agatcatgac aatgctgttt
     1141 catagttcag aacagatatt taaaccgtat tttaggaact aaaggagcat tatgaaaaag
     1201 attttattgg ttttacttat ggggttgttg ctgagctttc tatttgccaa atccagtttt
     1261 ttctgggttg aagatgttcc ctgggatgat ggcaaggcac tgataatcaa ggcaaatgtt
     1321 cctgctgata ctgtttatct gcaaaaagct cttgaggatg gaagctggca aacattatat
     1381 gctggcaaag gtataaacct tgagtataca gatagtgagc ttaatcccga tttagaatat
     1441 agataccggt tactagttcc ccgtcctact tttggtgata gtatttcttt taaggcaata
     1501 gcgaaaccta ttgagcaatg gttctatttg cataaactct cctatctatt aattttgctg
     1561 gctgtttctt tcgctattgt ttattatgtg caaaaagcca ggaaggaaag gattattata
     1621 ttcgccggat caacggctta gatagtatcg atgaatctat tggcagagcc acagaaaagg
     1681 gtaaacctat tcttttcgtt ccaggtattg gcgatttaga tgatattcaa actatttcca
     1741 gtttaactat cttaagccat ttagcccata aagccgcaga atatgatacc gagattattg
     1801 ttccctgtag gttttcaatg gttttatctg cctctcgaga agtagtaaaa gaagcatata
     1861 taaaagctgg gaaacctgat gcttacaatc aggatagaat tttttactta actgatgatc
     1921 aatttggtta tgtagcagga attgatggaa ttatggtacg tgaacaacct gcagctaatt
     1981 tttttctggg aagtttttat gctgaatctt taatcttagc ggaaaccgga tttagtactg
     2041 gtgcaattca gactgctgga accgcacagg ccagtcaatt accttttttt gtggtctctt
     2101 gtgattacac cttaattgga gaagaacttt ttgctgcatc tgcttattta tcaagagatc
     2161 ctcaacaact gggttcacta aaaggccacg attttggaaa actgttagtt atccttctta
     2221 tagtggctgg agttatagca gaaatgtcta attggcattg gttcaaagca ttttttgagg
     2281 gggcacaatg aagtataaag tccctcttat ctttgccttc gtttgtggaa ttctggtagt
     2341 tatttcagaa tttatccctc accgtccttt caatatgctg atcacaactt tacaagattg
     2401 gtttatgatt atttcaggtt ttgcaattat cttgggacaa ataagtttgt ttcaagcaaa
     2461 cattaagaaa atcagaggga aaaacccaga ttggccattt tatcttgctg ggctcattag
     2521 tttttcaatt atgttaatag tagggctttt ttggggaact caagagtata gaggaattct
     2581 gggtcatgga gataaaatcc aggcagcgat tggaatgaaa ccctttgatt acctatttgc
     2641 caatgccttt atgcctcttt cctcaacaat gttttctctt ttagcttttt ttattgcttc
     2701 tgctgc
//
LOCUS       DAORHB010000168         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_45251_length_2677_cov_4.456140, whole genome shotgun sequence.
ACCESSION   DAORHB010000168 DAORHB010000000
VERSION     DAORHB010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45251_length_2677_cov_4.456140"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PKM80_05190"
     CDS             <1..805
                     /locus_tag="PKM80_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNV93341.1"
                     /translation="NHNGSVLFQKNIGTQIKTSPVVVDVNNDNIKEIVLIANNGNIYI
                     MDGMGVDLPNTPIGINTAVECTPVIARFDGGNNAGIIFGDSSGKLHSVRIDGTESPNF
                     PIEIGGILKVSPTLADIDGDNDLEIAFPTNSGLSIIDVKRGAQALLWPNYLGGFGRTC
                     NAYQPTPIVDPQLPALETVLYEAYPNPFNPETTISFNLSSPNLTELYIYNQKGQKVKT
                     LFSGNLESGNHKFVWNGQDDNGNSVSSGLYFYRMKSGKYSSTKKMILMK"
     gene            1259..2359
                     /locus_tag="PKM80_05195"
     CDS             1259..2359
                     /locus_tag="PKM80_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNV93342.1"
                     /translation="METLFTGLLGFQLKQVIMILIGLVLIWLAIKHKYEPTLLLPIGF
                     GTILANIPLSAAVGEHSPLGILFKAGIDTELFPLLIFIAIGAMIDFSPLLKNPFMLLF
                     GAAAQLGIFVTIVLAASLGFDIKEAASIGIIGAADGPTSIYVANRFAPNLLGAISVAA
                     YSYMALVPIIQPPVIRLLTTKKERCIRMDYHSGDVPQTVKIIFPIAITIIAGIFVPAS
                     LALIGFLMFGNLIRESGVLEGLSKTAQNELGNLVTILLGITIASKMQGELFLVPQTLL
                     ILALGLVAFIFDTAGGVLFAKLMNIFKKEKINPMIGACGISAFPMSARVIHQMGLKED
                     PFNFLLMPAVSVNVGGQIGSVIAGGLILALLA"
     gene            2370..>2677
                     /locus_tag="PKM80_05200"
     CDS             2370..>2677
                     /locus_tag="PKM80_05200"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNV93343.1"
                     /translation="MLKLKPREKELLEEAKKASANAYSPYSNYKVGCAVRTVDEQIIT
                     GCNVENASYSLTICAERNAIFKAISEGHKDFTEIAVFVDSEESFPPCGACRQVIYEFA
                     P"
BASE COUNT          814 a          467 c          531 g          865 t
ORIGIN      
        1 taatcataat ggcagtgtgc ttttccaaaa aaatatcgga acgcaaatta aaaccagtcc
       61 cgtcgtggtt gatgtaaaca atgataatat aaaagaaatt gtcctaattg ccaataacgg
      121 aaatatctat attatggatg gtatgggtgt agatcttccc aatacaccta ttggaattaa
      181 taccgctgtg gaatgtactc ctgtaattgc cagatttgat ggtggaaaca atgccggaat
      241 aatttttggt gattcttcag gtaaattaca ttcggttcgt attgatggaa cagaatcacc
      301 caattttcct attgagatag gtggaatttt aaaagtatct ccaaccctgg ctgatattga
      361 cggcgacaac gatcttgaaa tcgctttccc tacaaattcc ggactttcca ttatcgatgt
      421 taaaagaggc gcccaggcat tactgtggcc taattatttg ggtggttttg gtagaacttg
      481 taatgcgtat caaccaacac ctattgtaga tccacaactt ccagctttgg aaacagttct
      541 ctatgaagca tacccaaatc ctttcaatcc agaaactaca attagtttca atctcagtag
      601 ccctaatctt actgaactct atatttataa ccagaaagga caaaaagtaa aaaccttgtt
      661 cagcggaaat ctggaatccg gaaaccataa atttgtgtgg aatggacaag atgataacgg
      721 caacagcgtt tccagcggat tatattttta ccgcatgaaa tcaggaaaat atagctccac
      781 caaaaaaatg attctaatga aataatgaac cttatggaaa ggcgtgaaac ctggaatgaa
      841 catcctatga gatagaatat attataaaat ggtgtagaag ggaaaacaag ataccttctt
      901 caaaagaaaa gtcaggagcc attatgctta aatatctgtt acttagtaaa gaacatttgg
      961 gcatatatgt ttcctgactt ttgtctttat cttatctata gagtttgtag ctccgttatg
     1021 aatgtgcact gccaaacaaa ccgtaagaat gttaacggat attttaggta gtgatgattc
     1081 ctaactaagg tgtaatcctt accatatcaa tgccctaaac cttataacca aggagatctt
     1141 ttactgataa aatcaagttt cacttttcca tattcgtatt tttctccagc tacatttgct
     1201 tgacaaacaa acccttttca caatagtggc aaatagttaa taattaggag atttgtggat
     1261 ggaaacactg tttaccggac tacttggatt tcaattgaaa caggttataa tgattctaat
     1321 aggtctggtt ttaatctggc tagcgattaa gcataaatac gaaccgactt tattgttgcc
     1381 cattggtttt ggtacaatat tagctaatat acctctttct gcagctgttg gtgaacattc
     1441 tcccttaggg attttattca aagctggaat tgatacggag ttgtttccgc tattaatttt
     1501 tattgctatt ggagcaatga tcgatttcag ccccttgcta aaaaatcctt ttatgctatt
     1561 atttggagct gcagcacaat taggcatttt tgtaaccatc gtgttagcag cttccttggg
     1621 ttttgatatt aaggaagcag ccagtattgg aattattgga gctgcggatg gacccacttc
     1681 tatatatgtt gccaatcgtt ttgctcccaa tctgttagga gcaatttctg tagcagctta
     1741 ttcttatatg gcattggtgc caattattca gccaccggtt attcgcttgc ttacaactaa
     1801 gaaagagcgt tgtataagaa tggattatca tagtggtgat gtacctcaaa cagtgaaaat
     1861 aatatttcca atcgcgataa caattatagc cggcattttt gtgcctgcct cgttggcgtt
     1921 aatcggtttt ttaatgttcg gtaatttaat tcgtgaatcc ggtgtattag aaggtctcag
     1981 caaaaccgct caaaatgagc tgggtaattt agttactatc cttttaggga taacaattgc
     2041 gtccaaaatg caaggtgaac ttttcctggt tccccaaact ctgttaattt tagcattggg
     2101 attggttgcc tttatttttg acacagcagg tggtgtgcta tttgccaaac tgatgaatat
     2161 attcaaaaag gaaaagataa atccgatgat tggagcttgc ggaatttccg cttttccaat
     2221 gagtgctcgc gttattcatc agatgggttt gaaagaagat ccttttaact ttttattgat
     2281 gccagcggta agtgtaaatg tgggaggaca gattggttca gttattgctg gtgggttaat
     2341 tttggctctt ttagcttaga ggatataaaa tgctaaaact gaaaccaagg gaaaaagaat
     2401 tgctggaaga agccaaaaaa gcatctgcca atgcttattc tccctattcc aactataaag
     2461 tcggttgtgc tgtaagaact gttgatgagc agataatcac tggttgtaat gtggaaaatg
     2521 cttcatattc tttaactata tgtgctgaac gtaatgccat ttttaaagca attagcgaag
     2581 ggcataaaga ttttactgag atagccgttt ttgtggatag cgaagaaagc tttcctcctt
     2641 gcggagcttg tcgtcaggtt atttatgaat ttgcccc
//
LOCUS       DAORHB010000169         2674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_45325_length_2674_cov_3.889653, whole genome shotgun sequence.
ACCESSION   DAORHB010000169 DAORHB010000000
VERSION     DAORHB010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45325_length_2674_cov_3.889653"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /gene="lepB"
                     /locus_tag="PKM80_05205"
     CDS             <1..340
                     /gene="lepB"
                     /locus_tag="PKM80_05205"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNV93344.1"
                     /translation="RIEYFTYPENSFDYVSMNPWYEPYRIKPEGDSTAVRKIFNRDWF
                     GPIKVPENSYFVMGDNRDVSEDSRYWGFLERKHITGTPWLIFFSRGIEFNKLYDEPHI
                     RWNRIFRHPS"
     gene            310..1434
                     /gene="hemW"
                     /locus_tag="PKM80_05210"
     CDS             310..1434
                     /gene="hemW"
                     /locus_tag="PKM80_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNV93345.1"
                     /translation="MEQDFQASQLGLYIHIPFCLTKCGYCSFFTLPFRQSSLYEYVGY
                     IEKEKVLYRYQLKRPLATIYFGGGTPSLLSAEQIMAILEGLNILPDAEITLEINPLQI
                     TEQYLKALKTTPVNRLSIGMQSMNNSELAWLNRHHNMEQIIDKIALCRKENFNNISLD
                     LIYGLPNSSVQTVQQTVDAYLKLQPEHISCYLLTLDEDCPLADEIDKLPNEDILAEQY
                     EYLCSHLKQAGYIHYEISNFAIPGFESKHNLRYWKSEDYLALGVSASGWIYPMRYQNP
                     VDINAYYENVKNRVIFPQKEELSNQRIIQDYLMMGLRLREGINLQEFNERFQIPLMEI
                     YREQINKLIKIKMLTLQEGNLALTEQALFVSNRVIGDLIL"
     gene            1431..2168
                     /locus_tag="PKM80_05215"
     CDS             1431..2168
                     /locus_tag="PKM80_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNV93346.1"
                     /translation="MNLMEWRILLDKLINDSDFAHKSYFAGGCVRDFLLNPDSSITSD
                     IDICVEIFEGGIALAKYLQNYLPESDIIIHQSFGTASLRYKNYKLEFVATRKEKYYPG
                     SRYPQVQFGNLLDDVLRRDFTINALLMSITTGEILDLSCKGLSDLKQGIIRTVQDPDK
                     SFSEDPLRMLRALQFALRFNFQIEDKTLKAMQKNTPALKILSKRAIQEELNKIPESKR
                     QEAIAFIDKMNWKPYLKHKSETQNEQC"
BASE COUNT          912 a          473 c          476 g          813 t
ORIGIN      
        1 acgaattgaa tattttacct atccagaaaa cagctttgat tatgtttcaa tgaatccctg
       61 gtatgaacct tatagaatta agcccgaagg tgattccacc gctgttcgta aaatattcaa
      121 tcgcgattgg tttggcccaa taaaggttcc cgaaaacagt tattttgtaa tgggcgacaa
      181 tcgtgatgtt agtgaagata gtcgttattg gggtttttta gaacgaaaac atataaccgg
      241 gactccctgg ttgattttct tcagcagggg aattgagttt aataaacttt atgatgaacc
      301 ccatattcga tggaacagga ttttcaggca tcccagttag ggctttatat ccatattcct
      361 ttttgcctta caaaatgcgg gtattgcagt tttttcactc tgccttttcg acaaagttca
      421 ttatatgaat atgttggcta tatagaaaaa gagaaagtac tatatcgcta tcagttaaaa
      481 agacctttag ccacaatcta ttttggcggg ggaactcctt cccttttaag tgcagaacag
      541 attatggcta ttttagaagg cctaaatatt ttacctgatg ccgaaatcac tctggaaatt
      601 aatcctctgc aaattacaga acaatattta aaggcactga aaacaacacc tgtaaatcgc
      661 ttgtctatag ggatgcaaag tatgaataat tcagaacttg cctggctgaa tagacatcat
      721 aatatggaac aaataataga taaaatagct ctatgccgta aagaaaactt caataatatt
      781 tccttagacc taatttacgg attgccaaat agttcagttc aaaccgtaca gcaaacagtt
      841 gatgcctatc tgaaacttca accggaacat atatcctgct atcttttaac ccttgatgaa
      901 gattgtcccc tggccgatga aatagataaa ctgcccaacg aagatattct ggctgaacaa
      961 tatgaatatc tttgtagcca tttgaaacaa gcagggtata ttcactatga aatatccaat
     1021 tttgctatac caggctttga atcaaaacat aacttaagat actggaagag tgaggattat
     1081 ttggctttgg gagtttctgc ctcaggatgg atttacccta tgcgttatca aaatcctgta
     1141 gatatcaatg cctattatga aaatgtgaaa aatagagtta ttttccccca aaaggaagaa
     1201 ctctctaacc aaagaattat tcaggactat ttgatgatgg gattgcgttt gcgagaaggg
     1261 attaacctac aagaatttaa tgagcggttt cagattcccc ttatggaaat atatcgtgag
     1321 cagataaata agctgattaa gataaaaatg cttactctgc aagaaggaaa cttagcttta
     1381 acagaacaag ccctttttgt ttccaatcgc gtaattggag atttaatatt atgaatttga
     1441 tggaatggag aatacttctt gataaattga ttaatgatag cgattttgcc cataaaagtt
     1501 actttgccgg gggctgtgtg cgtgattttc ttttaaatcc cgatagttct atcacaagcg
     1561 atattgatat ctgtgtggaa atttttgaag gaggaattgc cctggccaaa tatctacaga
     1621 actatctgcc ggaaagtgat attattatcc atcaaagttt tggaactgct tccttgcgct
     1681 ataaaaacta taagctggaa tttgtggcta cccgaaaaga gaaatattat cctggcagtc
     1741 gttatcccca agtacaattt ggaaacttac tggatgatgt cttaaggagg gattttacta
     1801 ttaatgcatt acttatgtct attaccacag gtgaaatttt ggatctttcc tgtaaaggac
     1861 ttagcgattt gaaacaagga attattcgca cagtacaaga cccggataaa agtttttcag
     1921 aagacccttt aaggatgcta agagcactgc aatttgcttt acgctttaat tttcaaatag
     1981 aagataaaac actaaaagca atgcaaaaaa atactcctgc cctgaaaatc ctttctaaaa
     2041 gagcaataca agaagaacta aataaaatcc ctgaaagcaa acgacaggaa gcaattgcgt
     2101 tcatagataa aatgaattgg aaaccctatc taaagcataa aagtgaaacc cagaatgaac
     2161 aatgttagct atattaatat agcatatcag cacaccagaa gcgagaggta aagacagaaa
     2221 gtacaccctc cctttatttt ttattcctat taagattcat ataaatatca attacaattg
     2281 caaggattaa agtaacagca taaaatacta cgaaaactcc cctcttgaaa ctgcattata
     2341 cttactgtaa caatcaattt gataataaaa tcccgatatt tttgtttcct actttccctg
     2401 ctgggagcca ttaatctatc gtttatctac ccttaatcat gcgttaacaa ttaacgcttg
     2461 attaaggctt gattaacact tgattaacgc tttccattaa ggccgaagag cgaagcttga
     2521 atacaatatg cagtgtatat taaagtggta aatacctgat gacagatata tgtaatagga
     2581 tagtacttaa atatccaccc ttcattttac tgcataagga aaaaagagct taccattttt
     2641 tgctgggcaa ataaaccgaa gtttgtcaac agac
//
LOCUS       DAORHB010000170         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_48247_length_2568_cov_3.518106, whole genome shotgun sequence.
ACCESSION   DAORHB010000170 DAORHB010000000
VERSION     DAORHB010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48247_length_2568_cov_3.518106"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(902..1930)
                     /locus_tag="PKM80_05220"
     CDS             complement(902..1930)
                     /locus_tag="PKM80_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV93347.1"
                     /translation="METIKIGIIGCGRISKNHFDAVSQIQEAEFVAACDVIDSRLQTV
                     SDNYGIKKLYTNYKEMMEKENLDLVSICTPSGLHPQIGIDAANHRINVLTEKPMATNI
                     EAADALIRACDANNVKLFVVKQNRLNSTMQLLKRAIDKGRFGRIYLAESNVFWQRPQA
                     YYDAEKWRGTWEFDGGAFMNQASHYVDALYWLLGNVDNVMAYTATMARRIEAEDTGCA
                     ILHFRNGIIATLNVTMLTFPKNFEGSITIIGEKGTVKIGGVAVNKIEKWEFEDYDDDD
                     RIAQDANYQPPNVYGFGHNPYYRNVIDVLLGKDVPSTDGRDGRKSVEIIQAIYRSAKT
                     GKRISLPL"
     gene            complement(1948..>2568)
                     /locus_tag="PKM80_05225"
     CDS             complement(1948..>2568)
                     /locus_tag="PKM80_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV93348.1"
                     /translation="PQGEANEDFPYKPDSAYGASKLAGELLCIKWQQENPKRELVVLR
                     PAAIFGPRNITNTYKLIDTLHRRPYLTIGEGNHIKTIVSIYTVIDMTCFAIQQLKPGF
                     EHYNCIDEPYLTLKELMELICSNPGFKMPRIKIPLKAAIGIGYLFDIPAKLFSIDIPV
                     NSDRMRKLGTATYFTAEKAKKAGFIQRVPLKDSIVEMCNWYLSLNE"
BASE COUNT          788 a          480 c          455 g          845 t
ORIGIN      
        1 aataccaaaa ggaagatggt agattttcac ttagcttttg tttttttccg ggattcaata
       61 tgcaagataa tgatcctaac atatactcta accatatagg gcttgataca ttcccgtatc
      121 attcccttat ccgatatggg aatcataagg gaatgctatg ggatgcttat gagtttcgaa
      181 taagttatat gtttcatcct aaataagttg acacaagaac aaagaaagga gatacagaaa
      241 tgagtgaggt gtcctgaaaa tagcggacta aaattaagga gaaagatatg cgtaaaaaaa
      301 tctacttaaa aggaagtata ttaaagatag cgataccttc gaacctaaag ttgctgatat
      361 ctgtggaatc tgcgtgagtt atttttggaa agattgacat cctgtctctc ctcttacagt
      421 caaattgtgt aattcccgca atattgaatg atagctttat agtgtgtttg ttgtgggttg
      481 atgaaaacat caaccttcct gtattttagc caggttaaaa tccatcgctc aaatctgtcg
      541 tcccgatggg acttttttta ttcgttcttc ttgtcgccgg gttaaaaccc gtcgttagta
      601 tgtatcgttc ctaatggata ttaaattaga aatcctctta atcctaaaat cctaaaatcc
      661 ttgtttctaa aattatatct gcgtaatcag cgtaatctgt gtgaaaaatc ttcggtgaca
      721 tcggtggcta ggaaaaaatg cggaggaacg gcgtcctccg tctacacatc taaatcctct
      781 taatccttgt ttctaaagtt atatctgcgt aatcagcgta atctgcgtga aaaatcttcg
      841 gtgacttcgg tggctagaaa aaaatgcgga ggaacgaaat cctccagcta taaattacag
      901 tttataaagg tagagaaata cgcttaccgg tttttgctga acgataaatg gcctgaatga
      961 tctcaacaga ttttctgcca tcgcgtccat cagttgaagg tacatcttta cctaaaagta
     1021 catctatcac attgcggtaa taggggttat gtccgaagcc ataaacatta ggtggttgat
     1081 aattagcatc ttgggcaatg cgatcatcat catcataatc ttcgaattcc catttttcta
     1141 ttttgttaac ggctactcct ccaattttca cagttccttt ttctccaatt atagttattg
     1201 atccttcaaa atttttggga aaagttagca tagtaacatt taaagtggca ataataccat
     1261 tccgaaaatg gaggattgca caacccgtat cttcagcttc aattctgcgt gccatagttg
     1321 ctgtgtaagc cataacatta tctacatttc ctaaaagcca ataaagtgca tctacataat
     1381 ggcttgcttg attcataaaa gcacctccat caaattccca tgtcccgcgc catttttctg
     1441 catcataata ggcttgaggt ctttgccaaa aaacatttga ttcagctaaa tagattctgc
     1501 caaagcgtcc tttatcaatt gctcttttta gtagttgcat agtagaatta aggcgatttt
     1561 gtttaacaac aaaaagttta acattattgg catcacaagc tctgattaga gcatcggctg
     1621 cttcgatatt agtagccata ggtttctctg ttaacacatt aattctatga ttggctgcat
     1681 caatacctat ttgaggatgc aagccacttg gggtgcagat agaaacaaga tctaaatttt
     1741 ctttttccat catctcctta taattggtat aaagtttttt aattccgtag ttatcggaaa
     1801 cggtctgcaa tcttgaatca ataacatcgc aagcagcaac gaattcagct tcctggattt
     1861 gagaaacggc atcaaaatgg tttttagaaa tacggccaca gccaattatt cctattttaa
     1921 ttgtttccat aataaaactc cttcatatta ttcatttaaa gatagatacc aattgcacat
     1981 ttcgacaatg ctatctttta gtggaactct ttgaataaaa cctgcttttt ttgctttttc
     2041 agctgtaaaa taggtagcag ttcccaattt acgcattcta tcactgttta caggtatgtc
     2101 tatggaaaaa agtttagcag gaatatcaaa cagatatcca ataccgatag ctgcttttaa
     2161 aggtatttta attcgcggca ttttaaaacc gggattacta catataagct ccatcagctc
     2221 tttaagagtt aaatacggtt catctatgca gttataatgc tcgaatcctg gttttaattg
     2281 ctggatagca aaacaagtca tatcaataac agtatatatt gaaacgatag tttttatatg
     2341 atttccctca cctattgtta aatatggtct tctatgtaga gtatcaatca gtttataagt
     2401 attggtaatg ttgcgaggtc caaaaatagc tgcagggcgc aatactacaa gttctcgttt
     2461 cggattttcc tgttgccatt ttatacaaag gagttcacca gccagcttgg aagcgccata
     2521 agcactgtca ggtttataag gaaaatcctc atttgcttct ccctgagg
//
LOCUS       DAORHB010000171         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_50013_length_2512_cov_3.730973, whole genome shotgun sequence.
ACCESSION   DAORHB010000171 DAORHB010000000
VERSION     DAORHB010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50013_length_2512_cov_3.730973"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="PKM80_05230"
     CDS             <1..1799
                     /locus_tag="PKM80_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV93349.1"
                     /translation="YNLPTVYVIVDKPSEAIGSLHRIDSEDVKTSLSLREAMQNSVGI
                     SATTGSKDESNLRLRGFRKNEIKIMVDGRPLNNGYFGNVDISKLSLLNIEEIQILKGP
                     ASPLYGTNNMGGVVNIITKAPPKNYWLELNTIFKRNNTNEIKLSSAHSFDSWNYRIGL
                     SREQTDGFNLSKDFIPTSSENGGIRNNSDKCLYNLSGSWESDLLNFHNIGLDFGYSTM
                     DKKNIPSSIYEHRYRQYQDWMRYYSGLWTKFQMNESSVVNLQLTYDTSADRYLEYNDP
                     TYETMTLDSQMRNQAFGVHPRLTKVFLGNRKLDIGYHYEFQLNRRKDNGSYLEWTYSP
                     VHLHQFFSQYECFLYPDLLLTTGLGLSAGINKYHNDFEPQLEPAVGITYDSSFNSKTS
                     FAIGRNTSQPTLRQLYSESKGNPALKPQHSLKVELSHNQALLKNKLSIYCSVYYNDTK
                     DLIDMYLGRYENIFKVQSQGAEIGMDISLLKFWQSSVNYAYLSYQENSDYYLTETPKN
                     SVELIQRIKLPFNISFIFDSSFRDERLSQDDEGRYHNLDPYWKHDLLITIPYKQIELS
                     VGMENILDENYQCEYGFPEPGRNISIGFQIKI"
     gene            2079..>2512
                     /locus_tag="PKM80_05235"
     CDS             2079..>2512
                     /locus_tag="PKM80_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV93350.1"
                     /translation="MIFKRESALLLQKYFETRFEFIDQGLEQALSFEDEYAANLKKAM
                     YEAVIPGGKRWRPILLLSMFEMLTSFKKHRAFPDAVKAAVAIELMHNGGLIHDDLPCI
                     MNRTQRRGQPALHIKYGNAIAILAADALYSLAFEILGEFQNS"
BASE COUNT          841 a          436 c          462 g          773 t
ORIGIN      
        1 cctataatct gccaactgtt tatgtaattg tagacaaacc gtctgaagca attggttcgt
       61 tacatagaat agatagtgag gatgttaaga cttcgctttc tttgcgggag gcaatgcaaa
      121 acagtgtagg tattagtgca acaacaggta gtaaggatga aagcaactta cgcttacgcg
      181 gttttcgcaa aaatgaaata aaaattatgg tggatggcag acctttgaat aacggatatt
      241 ttggcaatgt ggatatcagt aaactttccc tgcttaatat tgaagaaata cagattttaa
      301 aaggtcctgc ttccccttta tatggaacaa ataatatggg cggggtagtt aatattatta
      361 ccaaagcccc gcctaaaaat tactggctgg aactgaacac gatttttaaa cgcaacaaca
      421 ctaatgaaat taaactatcc tcagctcaca gttttgatag ttggaattat agaatcggtc
      481 tttcgaggga acaaactgac ggctttaatc tctctaagga ttttattcct accagttccg
      541 aaaatggagg aatccgcaat aatagcgata agtgtttata caatctatcc ggttcatggg
      601 aaagtgattt gttaaacttt cataatatcg gcttggattt tgggtatagc acgatggata
      661 aaaagaatat ccccagttcc atatatgaac atcgctatag acagtatcag gattggatgc
      721 gttattacag cggcttgtgg actaaatttc aaatgaatga aagcagcgtt gtgaatctgc
      781 aattaactta tgataccagc gcagaccgct atctggaata taatgatccc acttatgaaa
      841 cgatgacttt agattcacag atgcgcaatc aagcttttgg tgtacatcct cgtttaacta
      901 aggtattttt aggtaacaga aaactggata ttggttatca ttatgagttt cagctaaatc
      961 gtcgtaagga taatggttca tatcttgaat ggacttattc acctgtccat ctacatcagt
     1021 tcttcagtca atatgaatgc tttctttatc ctgatttatt gttaacaacc ggattgggct
     1081 taagtgcagg aataaataaa taccataatg attttgaacc gcaattggaa ccagcagttg
     1141 gaattactta cgacagctct tttaatagta aaacctcttt tgccattgga cggaatactt
     1201 ctcagccaac tttaagacag ctttattctg aaagtaaagg taatcctgct ttgaaaccac
     1261 aacactcact aaaagtagaa ctaagccaca atcaagcctt gctaaaaaat aaactctcaa
     1321 tttactgcag tgtttactat aacgatacca aagacctcat tgatatgtat ttgggacgct
     1381 atgaaaatat atttaaagtt caatcacaag gcgcagaaat tggtatggac atctctttgc
     1441 ttaaattttg gcaatcgagt gtaaattacg cttatctcag ctatcaggaa aatagtgatt
     1501 attatttaac tgaaacaccc aaaaattcag tagaattaat acaacgaatc aaactacctt
     1561 tcaatattag ttttattttc gacagctctt ttagagatga acgcctctcc caagacgatg
     1621 aagggagata tcataatctg gatccctatt ggaaacacga tctgcttatt actattccct
     1681 ataaacaaat agaactgtct gttgggatgg aaaatatcct ggatgaaaac tatcaatgcg
     1741 aatacggatt tccagagcca ggaagaaaca tctcaatagg ttttcaaatt aagatttagg
     1801 atataaaaaa ggaatcactc tttttattca cttattgcaa tttttgttgt cactcataat
     1861 ccactcatac acttgcaaaa ctcaattatg agtggatttt gggttgataa ccagctataa
     1921 caatccttgg atagataaac cctataggct ttacattaat tggtagcttc catacttcaa
     1981 ttatatgtac acttataatt cactgaaggg agaaaaaact tgacaaaagt atggtacgat
     2041 ataaaaagga agagtaaaat acagttattt taaactaagt gatatttaaa agggagagtg
     2101 ctttgctttt acaaaagtat tttgaaacac gtttcgagtt tattgatcaa ggactggaac
     2161 aggcattatc attcgaagat gaatatgctg ccaacctgaa aaaagctatg tatgaagctg
     2221 ttattcccgg aggtaaaaga tggcgtccta tcctgcttct ttctatgttt gaaatgctta
     2281 ccagttttaa aaaacaccgt gcctttccag atgcagtaaa agctgcggta gctattgaat
     2341 tgatgcataa cggcggctta attcatgatg accttccttg tataatgaat agaactcagc
     2401 gaagaggtca acctgccttg catataaaat atggaaatgc catagctatt ttagctgcag
     2461 atgcactata ttctcttgct ttcgaaattt tgggagaatt tcaaaattcg ga
//
LOCUS       DAORHB010000172         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_50275_length_2505_cov_2.801633, whole genome shotgun sequence.
ACCESSION   DAORHB010000172 DAORHB010000000
VERSION     DAORHB010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50275_length_2505_cov_2.801633"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKM80_05240"
     CDS             complement(<1..763)
                     /locus_tag="PKM80_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNV93351.1"
                     /translation="MLKHHILVFSLMLMLVLPFTLSGISQAAMLFLTFEPGARANGMG
                     RAYSAIADDAYSMWWNPGATAFNKDTQIAATHIPWLQGSGIEDIFYEYLGFNHYFYGI
                     GNINAHIIWLDAGTQTQTSSSNVDLGDFHTYEVSGALGYGYEVVPDKIGVGGNFKLLY
                     SYLGPSAGFTESEGSAFSFAFDAGALFKDVVVDNLNASIVLQNIGPDITYSDEEQADP
                     CPMTFRAGLGYTVFDNPMGRLITTAEMSKILANEDP"
     gene            complement(952..1038)
                     /locus_tag="PKM80_05245"
     tRNA            complement(952..1038)
                     /locus_tag="PKM80_05245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1002..1004),aa:Leu,seq:caa)
BASE COUNT          846 a          453 c          436 g          770 t
ORIGIN      
        1 aagggtcctc atttgccaga attttactca tctctgcagt tgtaattaaa cgccccatag
       61 gattatcaaa aacagtataa ccaagaccgg ctctaaaagt catcgggcaa ggatctgctt
      121 gttcttcatc actgtatgtg atatccgggc ctatattttg taaaacgatg gaagcattca
      181 aattatctac tacaacatct ttgaacaatg cacctgcatc aaaagcaaaa ctgaaagcag
      241 aaccttcaga ttcagtaaat ccagcacttg ggccaaggta ggaatataaa agcttgaaat
      301 tgcctcctac ccctatttta tctggtacta cttcatatcc ataacctaaa gctccagaca
      361 cttcataggt atgaaaatca ccaagatcaa cattactgga actggtttgt gtctgtgttc
      421 cagcatcaag ccagattata tgcgcattaa tattgcctat accataaaaa tagtgattaa
      481 aacctaaata ctcatagaag atatcttcta ttccagaacc ttgtaaccac ggtatatgag
      541 ttgcagcaat ttgagtatct ttgttaaatg cagtagctcc aggattccac cacattgaat
      601 atgcatcatc tgcaattgca gaataggcac gtcccatacc attggcacga gcaccaggtt
      661 caaaagttaa gaataacatt gctgcctgag atattccaga cagggtaaaa ggtagaacaa
      721 gcatcagcat taagctgaat accaatatgt ggtgctttaa cattatacct ccataggctt
      781 tataaaactt atttcaggct acaaaaatat cgcctgaaga aaattccttt ttttatatat
      841 aatctaatct gcagaatgta aatatcatct tagattatgc ctcagttcca tcaactacat
      901 ataggggctt accctgttat caattaaata gtaataactc ctaaaaagct ttggtgccga
      961 aggccggaat cgaaccggca tgagctaatg ctcggtggat tttgagtcca ctgcgtctac
     1021 caatttcacc acttcggcat attattctat ccaaaaaatc aatatacact ttaatgtcaa
     1081 gcaaaaaggt gtttttatta tccccacttg aattgctgta gttaatatat tataatctat
     1141 ctcctataaa aaagcaaata tataaggcag ctgcataatt tgaaatcttg gtttaactct
     1201 catttataga atatagcgat aacaatatat tcggctataa ggggaacgac gagaacatcg
     1261 tacctcctaa aaatagttca cgcaagtatg aattatagaa acaaggattt aagaatttta
     1321 gaatttaggg gatttagatg tgtagtggag gacgctgttc ccctgcaaga aatatcctct
     1381 catagattac acagattatg catatatgca caacttcaag ttcgagggca tcgcgatctt
     1441 gaatattaca accttttaag tagaattttt gttcatttct ttctcaataa ttttagtcca
     1501 ccatattgaa gatggctcaa acaaacaacc catccctctc tcctcctcct ctcctcatca
     1561 acccatagtt aaggatgagt gaagatgggg tggatgatgt gttgtatctg ggttaccagc
     1621 aagaaaagta agggttagag caagccaact atttatatgt tgttaatctt ccaggtgtta
     1681 gcaaatctca atggcttcgc attatcgatc ttaatatgct ttttatcgtg atgataaatc
     1741 cactatatat tggaggtggt attatttcaa aagttggcgt aaaataaatt gggaaaacca
     1801 attggatatg tgcaataatg ttatctattg tatggattca aggtaatctc taactgacaa
     1861 ggaaaattgc tttgacattt tatcctctct ttattataag gcatttatgt taactaacaa
     1921 gtgagcttag taaagctaaa ctgcttctac taaggagaat acaatgctag ctaaactcta
     1981 taggaatact taccgttgct tagttaagtt cgagattgaa tttgattccc taaaattata
     2041 attggcaggt aagcatcgta tcctgcgatt aggatttaat aatggggaaa ttcaactatt
     2101 gcttacggaa ctttgtgatg tttgtagaat taagatattt taaattatcg gaattgattg
     2161 cccagggttc caaagatgag taaacaggaa aatgttgacc gtagtaatta gtactttgac
     2221 cctgaatatg aataaaccac aattgaatat atcaaaaata tacggtgtac ataatagtga
     2281 tgaagaatga tgttaagatc taaatatgtt gagattattc gtataaggaa gaggaacaag
     2341 atatgctaat attcaaggta ttacatagta ggaaagaact gcctgaaaac cttactcaaa
     2401 aggatatagt agatttcctt tacacacatt tggaacgatt cagagataca tcttctgcaa
     2461 tttctaaagc catcgattat gctttttcca atgaaccggg aaaag
//
LOCUS       DAORHB010000173         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_50397_length_2501_cov_4.502862, whole genome shotgun sequence.
ACCESSION   DAORHB010000173 DAORHB010000000
VERSION     DAORHB010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50397_length_2501_cov_4.502862"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="PKM80_05250"
     CDS             <1..1701
                     /locus_tag="PKM80_05250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNV93352.1"
                     /translation="SGPVSQNEICYIIQGGVKLMAEVIKVVGNVANTQVFESTRGLKP
                     GDQVEFTNHMLEVKLGPGMLSKNFDGLQGDLNKREGVYLTRGEYTDPLDEDSLWKFTP
                     IAKAGDKVSAGDWLGSVPENWVNHKIMVPFVMEDEYTVKSVVPEGEYKITDTIAVLTD
                     NDGKDIPVNMIQKWPVKIPITAYKEKPRPFKLLETGVRTMDTLNPMVEGGTGFIPGPF
                     GAGKTVLQHSISKNAEADMIIMAACGERANEVVELFVEFPELDDPRTGRKLMERTIIV
                     CNTSNMPVAAREASVYTAMTIAEYYRNMGLKVLLLADSTSRWAQALREMSNRMEELPG
                     PDAFPMDLPAIISNFYARAGFVYLNNGATGSITFIGTVSPAGGNLKEPVTESTKKAAR
                     CFYALSQARADSKRYPAVDPIDSYSKYLEYPEVIEYLNTNVNPHWVVDVNHTKDIILR
                     GKEAQEQINILGDDGVPLGYHDKYWKSELIDRVILQQDGFDKVDQSTPMNRQQYMLEL
                     ILEICNTNFVFESFEEVMPYFTRLINICKQMNYTEFQTAEFNNYEAELRKIVEERRAK
                     "
     gene            1701..>2501
                     /locus_tag="PKM80_05255"
     CDS             1701..>2501
                     /locus_tag="PKM80_05255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424783.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNV93353.1"
                     /translation="METQAFQKIYTKLTQITKATCSVQATGVGNEELATVAGRLAQVV
                     KIVGDNVTLQIFAGTEGIGTDAEVVFFGKAPTLKVSDELGGRFFNAYGEPIDGGPEIE
                     GKEVEIGGPSVNPVRRKQPSELIATGIAGIDLNNTLVTGQKIPFFADPDQPYNEVMAM
                     VALRAQSDKIILGGMGLSNDDYLLYKNTFENAGVIDRIVSFVNTTENPTVERLLVPDM
                     ALTAAEYFALEKHEKVLVLLTDMTLYCDALSIVSNRMDQIPSKDSMPGS"
BASE COUNT          790 a          425 c          551 g          735 t
ORIGIN      
        1 tctggacctg tttcccaaaa tgagatatgc tacattattc agggtggagt aaaattgatg
       61 gcggaagtta tcaaagtggt tggtaatgta gctaatacac aggtctttga aagcacccgc
      121 ggtttaaaac caggtgatca agtagaattt actaatcata tgctggaagt aaaattaggc
      181 cctggaatgc tttccaaaaa ctttgatggc cttcaaggtg atttaaataa aagggaagga
      241 gtttacctta ctcggggtga atatactgat cccttggatg aagattccct ctggaaattt
      301 acccctattg ccaaagcagg cgataaagta agtgctggtg attggcttgg ctctgttccc
      361 gaaaactggg ttaaccataa aattatggtt ccttttgtga tggaggatga atacacagtt
      421 aaaagcgttg tgcctgaagg tgaatataaa attactgata caattgctgt tttaacagat
      481 aatgatggca aagatatacc tgttaatatg attcaaaaat ggcctgtgaa aatccctatc
      541 acagcttata aagaaaaacc tcgccccttt aaacttctgg aaacaggagt gcgaacaatg
      601 gacactctaa atcctatggt ggaaggagga acaggtttta ttccaggtcc ttttggcgca
      661 ggaaaaactg ttttgcagca ttccatatct aaaaatgcgg aagcggatat gattattatg
      721 gctgcctgtg gtgaaagagc aaatgaagta gtggaacttt ttgtagaatt ccctgaactg
      781 gatgatccca gaactggaag aaaacttatg gaacgaacta ttattgtttg taacacttcc
      841 aatatgcctg tggctgctcg tgaagcatct gtatatactg ctatgacgat tgccgaatat
      901 tatagaaata tggggctaaa agttttactt ttggcggatt ccacttcacg ctgggctcag
      961 gcactgcgcg aaatgagtaa tagaatggaa gaacttcctg gtccggatgc tttccctatg
     1021 gaccttccag ccattatttc caatttctat gctcgggcag gttttgttta tctaaataat
     1081 ggagcaactg gttctataac ttttattgga actgtctcgc ctgcaggtgg aaacttgaaa
     1141 gaacctgtta ctgaatctac gaagaaggcg gcacgctgtt tttatgctct atctcaagcc
     1201 agggcagata gtaaacgcta tcctgcagtt gatccgattg attcttacag taaatatttg
     1261 gaatatccgg aagtgattga atacctcaat actaatgtaa atccgcattg ggttgtagat
     1321 gtaaatcata ctaaagatat tattctgcgc ggaaaagaag ctcaggaaca aattaatatt
     1381 ttaggagatg atggagttcc gcttggttat catgataaat attggaaaag tgaactaatt
     1441 gatagagtta ttttacaaca ggatggcttc gataaagtag atcaatccac tccaatgaat
     1501 agacagcaat atatgctgga actgattttg gagatatgta atacgaattt tgtgtttgag
     1561 agttttgaag aagttatgcc ttattttacc agattaatta atatctgcaa acagatgaac
     1621 tacacagagt tccaaactgc agaatttaat aattatgaag ctgaacttcg taaaattgtt
     1681 gaggaaagga gagcgaaata atggaaaccc aggcctttca aaaaatctat acgaaattaa
     1741 cccaaataac taaagctacc tgctccgttc aagcaaccgg agtcgggaat gaagaattgg
     1801 ctacagttgc tggacgttta gcgcaagttg tgaaaatagt tggtgataat gtaaccttac
     1861 agatttttgc aggaactgaa ggtattggaa ccgatgcgga agttgtcttt ttcggaaaag
     1921 ctcctacctt gaaagtaagt gatgaattag gtggtaggtt ttttaatgca tacggtgaac
     1981 cgattgatgg tggaccagaa attgaaggaa aagaagttga aattggaggt ccttcagtta
     2041 atcctgtacg ccgcaaacaa ccctcagaat taattgctac aggtattgcc ggaattgatc
     2101 taaataatac tttggttaca ggacaaaaaa tacctttctt tgcagacccc gatcaaccct
     2161 ataatgaagt aatggctatg gttgctttac gggctcaaag tgataagatt attttagggg
     2221 gaatggggct ttccaatgat gactacctac tctataagaa cacctttgaa aatgccggag
     2281 taatcgatag aattgtctcc tttgtgaata ctacagaaaa tcctactgtg gaaagattgc
     2341 tggttcctga tatggcactt acagcagccg aatatttcgc tttggaaaaa cacgaaaagg
     2401 tactggttct tttaactgat atgactctat attgcgatgc tttaagtatc gtttctaatc
     2461 gtatggacca aataccttcc aaggatagta tgcccggttc t
//
LOCUS       DAORHB010000174         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_52538_length_2435_cov_4.739076, whole genome shotgun sequence.
ACCESSION   DAORHB010000174 DAORHB010000000
VERSION     DAORHB010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52538_length_2435_cov_4.739076"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PKM80_05260"
     CDS             <1..523
                     /locus_tag="PKM80_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HNV93354.1"
                     /translation="ILADMGIKISLEEVISVAGSRELIVRPHIAQVLVKHGFCQTKNE
                     AFDKYIGNNKPAYVPKPEVSVAEAVSIIHKAEGVAVIAHPVKLTKLSYIDDFIPMGID
                     GLEVWHPDHYQWEIDNLINIAMKNGLYMTGGSDFHSEQDKHNLFDIIPLPEIVIQSIK
                     ELWKEYQCRALSR"
     gene            502..1038
                     /locus_tag="PKM80_05265"
     CDS             502..1038
                     /locus_tag="PKM80_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93355.1"
                     /translation="MPRTIKVKLIRNEFPATYRYSLWLRIFKLLFAFAVCLYAFYFVV
                     KFVSAETPMFFKVLPLIILFIALDGMFKQLMVLNRVTFYEDRVCFGFIAKPRLIIPYE
                     NILYIDLVKQITYSIILKYKEGNREKIFKTSASFPKILEIILNLADLAPNAQSSEFFG
                     KIIKHLRTLAGENDAEQV"
     gene            1022..2188
                     /locus_tag="PKM80_05270"
     CDS             1022..2188
                     /locus_tag="PKM80_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNV93356.1"
                     /translation="MQSRFDKIIDRRNSGCYKYDALSMIYGKSDLIPLWVADMDFAVS
                     DAILDALSERLEHPVFGYNFRLNDFSEAIVSWEQRRFNWNIHKDWIISVPGLVPALSL
                     FVLAFTEPGEGVLIQTPVYRPFYDAVIDHNRKLITSALINNNGVYSIDWDDFENKLKQ
                     AKLFILCSPHNPVGRVWTEEELCKIGSLCAKYKVIVFSDEIHADIVYPGYKHIPFASL
                     ADFSNFCLTGVSPSKSFNIAGLATAVVIISNSVIRDKFMVMNTKLHLYLGNSFGIKAL
                     IAAYNDSENWLEELLCYLNGNREYISNFVQNELPGVLLSPIEGTFLAWLDFRNWGLPE
                     PKLQEMIIKKAGLALEPGSEFGDKDEGFMRLNFGCPRSILELALSKIKLLAEDL"
     gene            2188..>2435
                     /locus_tag="PKM80_05275"
     CDS             2188..>2435
                     /locus_tag="PKM80_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNV93357.1"
                     /translation="MMEQEIIDLYKNPPQKYTEEHKALFMAFSDALNRGIVRSCEKKN
                     DTWIVNEWVKMGILTGFRMGELTAFPWSEKKPFFDKDT"
BASE COUNT          771 a          366 c          484 g          814 t
ORIGIN      
        1 tattttagct gatatgggga taaaaatttc cctggaagaa gtaatttctg ttgctggaag
       61 cagagaatta attgtgcgtc ctcatattgc tcaggtttta gttaagcacg gtttttgcca
      121 aacgaaaaat gaggcttttg acaaatatat tggtaataat aagcctgctt atgtgccaaa
      181 gccagaagtg tctgttgctg aagcagtttc cattattcat aaagctgaag gtgtagcggt
      241 tattgcccat cccgtaaaat tgactaaatt gtcttatatt gacgatttta ttccaatggg
      301 tatagatgga ttggaagtat ggcatccaga tcattatcag tgggaaatag ataaccttat
      361 taatattgct atgaaaaatg gtctttatat gacaggggga agtgattttc acagcgaaca
      421 ggataaacat aacttattcg atatcattcc cctgcccgaa attgttatac aaagtatcaa
      481 agaactttgg aaggaatatc aatgccgcgc actatcaagg taaaattaat tcgtaatgaa
      541 tttcctgcta cttatcgtta ttcattatgg ttgaggatat tcaaattatt atttgcattt
      601 gcagtatgtt tatatgcctt ctattttgta gtgaaatttg tttctgctga aacacctatg
      661 ttttttaaag tgcttccttt gataattctg ttcattgcac tggatggtat gtttaaacaa
      721 cttatggtat taaacagagt tactttctat gaagatagag tatgtttcgg gttcattgct
      781 aaacccaggc tgattattcc ttacgaaaat atcctctata tagatttagt aaaacagata
      841 acatacagta ttatactgaa atacaaagaa ggcaataggg aaaagatatt taaaacatct
      901 gcgtcgtttc cgaaaatctt ggaaattatt ctaaatttgg cagatttagc tcccaacgca
      961 caatcgagcg aattttttgg aaaaattatt aaacatttac gaactcttgc tggagaaaat
     1021 gatgcagagc aggtttgaca aaataatcga tcgtaggaat agtggatgtt ataaatatga
     1081 tgcgctttca atgatatacg gaaaaagtga tcttatccct ctttgggttg cggatatgga
     1141 ttttgctgtt tccgatgcta ttctggatgc tttatcagaa cgcctagaac atcctgtatt
     1201 tggatataac tttcgtttga acgattttag tgaagcaatt gtatcttggg aacaaagaag
     1261 gtttaattgg aatattcata aggattggat tatttctgtt ccaggtttgg ttccagcttt
     1321 atcattattt gttcttgcgt ttacagaacc aggggaggga gttcttattc aaactccagt
     1381 ttatcgtcct ttctatgatg cagttataga tcataaccgt aaactaataa cttctgcgtt
     1441 aattaataat aatggagttt acagcataga ttgggatgat tttgaaaaca aactaaaaca
     1501 ggcgaaattg tttattttat gtagtcctca taatcctgtc ggcagagttt ggactgaaga
     1561 agaactatgt aaaattggca gtttatgcgc taaatataaa gtaattgtct tttcggatga
     1621 aattcacgca gatattgtat atccaggtta taaacatata ccttttgcat ctcttgctga
     1681 tttttctaac ttttgtttaa caggggtttc tccttccaaa agttttaata tagctggttt
     1741 agcaaccgca gtagtaataa tatcaaattc agttattaga gataaattta tggttatgaa
     1801 tacaaagctt catctatatt tgggtaatag ctttggaatt aaagctctaa ttgctgctta
     1861 caacgattca gaaaattggt tggaagaatt gctctgttac ctaaatggaa accgggaata
     1921 cataagtaac tttgttcaaa atgaattgcc gggagtttta ctcagcccaa ttgaagggac
     1981 ttttctagca tggctggatt ttagaaactg gggtctgcca gagcccaagt tacaagaaat
     2041 gataattaaa aaagctggtt tggcacttga acctggaagt gaatttggag ataaggatga
     2101 aggatttatg cgtttgaatt ttggttgtcc acgcagtatt ttagagctgg ctttaagcaa
     2161 gataaaactt cttgctgagg acctttaatg atggaacaag aaattattga tctctataaa
     2221 aatccccccc aaaaatatac ggaagagcat aaagctctgt ttatggcttt ttccgatgcc
     2281 ttaaataggg gtattgttcg ttcctgtgaa aagaaaaacg acacctggat agttaatgaa
     2341 tgggtaaaaa tgggaatcct tactggtttc agaatgggag aattaaccgc cttcccttgg
     2401 agcgaaaaaa agcccttctt cgataaagat acaat
//
LOCUS       DAORHB010000175         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_53080_length_2420_cov_4.384778, whole genome shotgun sequence.
ACCESSION   DAORHB010000175 DAORHB010000000
VERSION     DAORHB010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53080_length_2420_cov_4.384778"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..2371)
                     /locus_tag="PKM80_05280"
     CDS             complement(290..2371)
                     /locus_tag="PKM80_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNV93358.1"
                     /translation="MAIFTLATINLFSYNKGDYRTKWGGNFEDLSLWEYYNGSGWENA
                     TQLPSSPFANTIYINTQNIAIHTSIIIEGGLVITGTLQLYSGIAVTINKDANCEIGTI
                     QIYSASKLINNGKIKNGNTNSSITVMDGGTLENNGLISNSNESQNCQVTANSNGIIIF
                     GSQGSITGNCSFTANYGSTIATSNPQGMDGSLTCSGTIIYNRPYLIFNGSEPQITGSK
                     IPEQVLGIDFNNPTGISLSKNIKLVYTSLVHSGTTLYFGMSVIEEAWWGSGTFSMEDS
                     SSIATANPDGFSSTGKTGSLQVGTRNYNSNGNYIFNGTVHQQTGDFNPAPNPYTVNDI
                     IFDNLAGVTLTNPITVVSTMELLNGDLSYNILPQGVDGYYSPDVQKVSIDKNGTLMYN
                     FMAESLPFQNNNAFVNRKWILKGNFNGTKKITFNWNEDEDNYFNWNVHNFPKVYSSNS
                     DEPLVTIWNPAHPRKISFIALSFLNAKDDVFYYIGRGMDDTLPVTLSSFIATQTSSST
                     VKLSWTTQSETNVMGFNILRSNQSNLDTATSLGYFTPATNTSQIRTYQCTDQVFATGI
                     YYYWLEVVDFNGTNSFYGPAIADIQNIGNQTPSIPVKEGFHSIAPNPIDYEARIFYGI
                     PSKSPTLIQVYNLKGQKVRTILNETKDSGNFHLLWDAKDDQGSQLANGNYILRMDASG
                     KVWTKKITILH"
BASE COUNT          755 a          424 c          441 g          800 t
ORIGIN      
        1 tctttatgaa agattcggct ttacttccaa gtatgctgaa atgcgttggc agaaacaata
       61 ttagggaaca cggattagag caaaatcaat aaaactataa ctacagtatt aatttattgc
      121 caattgtaat taccgtctat tactctaaat ataaagttat aaatctaaac tgagcttccg
      181 cttagaaaag aacagacagg aagtataaag atactattgc tctctttatc gcgttactat
      241 agaataaaga atcgattgag agaaaaaaca agacaataga tctaattgtt taatgcagga
      301 tagttatctt cttcgtccaa actttccctg atgcatccat gcgtaggata taatttccat
      361 tggctaactg gctaccttga tcgtctttgg catcccaaag tagatgaaaa tttccactat
      421 ctttagtctc gtttaaaatt gttctcactt tttgtccttt caggttataa acttgaatca
      481 aggtaggaga ttttgaggga attccataga aaattcttgc ttcgtaatca ataggattcg
      541 gtgctatgga atgaaatcct tcttttactg gaatggaagg agtttgattg cctatatttt
      601 gaatatctgc gatagctgga ccgtagaatg aatttgtacc attgaaatcc acaacctcca
      661 gccaataata gtatatgcct gtagcaaaaa cctggtcagt acattggtat gttctaattt
      721 gggatgtatt cgtagctggt gtgaaatagc ccaggctagt tgcagtatcc agatttgact
      781 gattggatct taagatatta aagcccatca catttgtttc cgattgggtt gtccaactca
      841 atttgacagt agagctacta gtttgcgtag ctataaatga acttagagtt accgggagag
      901 tatcatccat tcctcttccg atatagtaaa atacatcatc tttggcattg agaaagctaa
      961 gagcgataaa agagatcttc ctggggtgag caggattcca aattgttact aatggctcat
     1021 cactgttgga tgaatacact tttggaaagt tatgcacatt ccaattaaag taattatctt
     1081 catcctcatt ccaattgaag gttatctttt tggtgccgtt gaaattacct ttcaatatcc
     1141 atttacggtt aacaaatgcg ttattatttt gaaagggaag cgattccgcc ataaaattgt
     1201 acatcaaagt gccatttttg tcgatggaaa ccttttgtac atctggactg taatagccat
     1261 ctactccttg gggcagaata ttataactaa ggtctccgtt aagaagttcc atagtagaaa
     1321 ctactgtaat agggttggtc aaagtaacac ctgcgagatt atcaaatata atatcgttta
     1381 cagtataggg atttggtgca ggattaaaat ctccggtttg ttgatgaaca gtgccattaa
     1441 aaatataatt gccattgctg ttataatttc ttgtgcctac ttgcaaactt ccggtttttc
     1501 ctgtagaaga aaagccatcg gggttagcgg tggctattga agaactatct tccatagaaa
     1561 aagttcctga accccaccat gcttcttcaa taacagacat accaaaatag agagtggttc
     1621 cgctatgaac caaagaagta tatacaagtt tgatattctt acttaagctt atgccggtag
     1681 gattattaaa atctatacct aaaacctgtt ccggtatttt tgatccggta atttgaggtt
     1741 ctgaaccatt gaatataagg taaggacgat tatagatgat tgttccgcta caagttaagg
     1801 aaccatccat accttgtgga ttagaagtag ctatagtgct tccgtaatta gcggtaaaag
     1861 aacaattccc tgttattgaa ccttggctac cgaagatgat tatcccattg gaatttgctg
     1921 taacctggca attttggctt tcattagaat tagatataag accattgttt tcaagggttc
     1981 cgccatccat aacagtgatt gatgaattgg tattaccgtt ttttatttta ccattattaa
     2041 tcagttttga agcactgtat atttgaattg ttccaatctc gcaattagca tctttattga
     2101 ttgtcactgc aattccagaa tataattgta aagttccagt tataactaga ccaccctcaa
     2161 taattataga agtatgtata gctatgttct gagtgtttat atagatggta ttggcaaaag
     2221 gagaagatgg caactgagta gcattttccc atcctgaacc attataatat tcccataaac
     2281 taaggtcttc aaaattacca ccccattttg tgcgataatc tcctttgttg taactaaata
     2341 agttaattgt agccaaggta aagatagcca taactaccaa ccatttaatc ctagttcttt
     2401 ttgctgtttc ataatcactg
//
LOCUS       DAORHB010000176         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_53194_length_2417_cov_4.928874, whole genome shotgun sequence.
ACCESSION   DAORHB010000176 DAORHB010000000
VERSION     DAORHB010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53194_length_2417_cov_4.928874"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1491)
                     /locus_tag="PKM80_05285"
     CDS             complement(256..1491)
                     /locus_tag="PKM80_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV93359.1"
                     /translation="MVIISGCGNKKSPTGGPQDIEKPTVLATSPIEFGDISSGVIEIS
                     FSKPMDKNSLANSIYIYPPVQNKKITLDKSNLKIRLNEPLRANTNYFITLTKRLKDIY
                     GNSLVNNQTLIFRNGKLATNRIAGIIEYEEPSDKGLPLEVTVLSLDSLLVISNVIRGG
                     AYAIDNLNPQKHILRSYIDKNQNSRYDFGVEPFYEGISDGSPLANMNISMTYSDTTLA
                     RINKINVNSSRELQIVLSKDITDIKDISIYSKDNPLHIAHQLIEGNIINLLTAKMDST
                     EYQLILTQVKDNKNNIKDKLEAKFQAKPLPDTLAPKITYTNPRNGASVNTLTPVLEIH
                     FNEIIPSSNVKAKLLCSNQEIPIKHLSGTERIHRFQPTKELENYKSHILIITSETTDF
                     SGNHLKNKYELQFLPLQRK"
     gene            complement(1526..2128)
                     /locus_tag="PKM80_05290"
     CDS             complement(1526..2128)
                     /locus_tag="PKM80_05290"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNV93360.1"
                     /translation="MFTGIIEATEKIISIQHFGRKKLISISKPAIFSDIEIGSSIACD
                     GICLTIVKLDNRTFTVEIMNETIQKTTAKNWSINKLLNLERALTITSRLNGHFVQGHI
                     DRELRLISHLIIEGTSYLRFEFPFREKELIVPQGSIALNGVSLTIAEIRTEYFSVAII
                     GHTLEHTNLNTIKPGEMVNVEFDIIGKYIIAQKDKIYELE"
     gene            complement(2130..>2417)
                     /locus_tag="PKM80_05295"
     CDS             complement(2130..>2417)
                     /locus_tag="PKM80_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNV93361.1"
                     /translation="FVLGHLLSPLDGYGPEQINSQQLMERILTLHPTEIILALKPSAE
                     GEATIHYLWELLKDKEINITRLSTGIPFGGDLEYSNLNTLSNAWKRRYIVQ"
BASE COUNT          754 a          383 c          424 g          856 t
ORIGIN      
        1 tttcatttga aaagaaaaaa ggatgaggat tcggcttttt ggtcacttca taggcagttt
       61 atgaaagata atcccgagct tttcggatta gtggaaacaa aggattgaac agctattgag
      121 tatgatgatt tagtaatcaa caactctctt tttcccacat agtataaaac ttttatgaac
      181 ttactaaata ttaagttaga gaatcctata aatcagtata attctattta tatcagagtt
      241 actttcctgg tatatctatt ttctttgaag gggaagaaac tgtaattcgt atttgttttt
      301 tagatggtta ccactgaagt ccgttgtttc cgaagtaata ataagtatat gtgatttgta
      361 attttccagt tctttggtgg gctgaaaacg atggatccgt tctgttcccg acaagtgttt
      421 aataggtatt tcctgatttg agcagagcaa tttggcttta acatttgagg aagggataat
      481 ttcattaaag tgaatttcca gaacaggtgt aagtgtattt actgatgcac cgttacgagg
      541 attagtataa gtgattttgg gagccaaggt atcaggcaat ggtttagctt gaaatttggc
      601 ttccaacttg tccttaatat tgtttttatt atctttaacc tgagtaagaa tcagctgata
      661 ttcagtgcta tccattttag ctgttagaag gttaattata tttccttcaa tcagttgatg
      721 tgctatatgt aaaggattat ctttggaata tatgctaatg tctttaatat cagtgatatc
      781 cttgcttaat acgatttgca attcacggct actattaaca ttaatcttgt ttattcttgc
      841 caaggttgta tcactgtaag tcattgatat attcatattt gctaaaggag agccatcaga
      901 tatcccttca taaaatggct caacaccgaa atcgtaacga ctattttgat ttttatcgat
      961 ataggatcgc aagatatgtt tctgtggatt caaattatca atagcgtaag ctccacctcg
     1021 aataacatta cttatgacta aaagagaatc aagagaaaga acagtcactt ccaaaggcaa
     1081 acctttgtcg cttggttctt catattcaat aatacccgcg atacgattag tggctaattt
     1141 cccattgcga aagataaggg tttgattatt cactaaagaa ttaccatata tatctttaag
     1201 acgcttagtt aaagtaatga agtaatttgt atttgccctt aatggctcgt ttaaacggat
     1261 tttaagattt gatttatcca aagtgatttt tttattttgt acagggggat agatatagat
     1321 agaattggcc aatgagtttt tatccattgg tttagaaaag gatatttcaa taactcctga
     1381 agaaatatca ccaaattcaa taggagaagt agcaagaact gttggctttt caatatcctg
     1441 gggtcctccc gttgggctct ttttatttcc acatccactt ataataacca ttaatataag
     1501 caaatagcat agacgagata atgctttact caagttcata tatcttatcc ttttgtgcta
     1561 taatatattt accgataata tcaaattcta catttaccat ttcgccgggt tttatagtat
     1621 ttagatttgt atgttcaaga gtgtgaccta ttatagctac actaaaatac tcagtgcgaa
     1681 tttcagctat agttaaacta actccattaa gagcaattga gccttgagga acgatcagtt
     1741 ctttttccct aaaaggaaat tcaaaacgca gataagaagt tccttcaatt attaaatgtg
     1801 agattagtcg taattctcta tcgatgtgac cttgaacaaa gtgtccattc aagcgagaag
     1861 taatcgttaa agctctttcc agattcagta atttatttat actccaattt ttagcagtgg
     1921 ttttttggat tgtttcgttc ataatttcca cggtaaatgt tctattatcc aatttgacga
     1981 tcgttaagca aattccatca caagcaatgc tagaaccaat ttctatatca ctaaatattg
     2041 caggtttgga aatagatatc agttttttcc tgccaaaatg ttgaatgctg ataatttttt
     2101 ctgtagcttc gatgattccg gtaaacatac tactgtacta tgtatctacg tttccaggca
     2161 ttagataaag tattcaagtt actatattcc aagtctcctc caaaaggaat acctgtagaa
     2221 agtcgggtaa tattgatttc tttatcttta agcaattccc aaagataatg gatagttgct
     2281 tcaccttcag ctgaaggttt taaagccaag attatttcag ttggatgaag ggttaggatg
     2341 cgttccataa gttgctgtga atttatttgt tctggtccat atccatccag aggtgaaaga
     2401 agatgaccca aaacaaa
//
LOCUS       DAORHB010000177         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_53407_length_2411_cov_4.801783, whole genome shotgun sequence.
ACCESSION   DAORHB010000177 DAORHB010000000
VERSION     DAORHB010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53407_length_2411_cov_4.801783"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKM80_05300"
     CDS             complement(<1..316)
                     /locus_tag="PKM80_05300"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNV93362.1"
                     /translation="MSRTNESALQQEIMAWIKQNQYVYLATVDKNKARVRPVVLFIYD
                     DRFFVTTFTGDSKVSQIQNNKNIEVCIPLYEEGQTGYIRLSGTASIVHNATLKAEASE
                     RCF"
     gene            complement(329..1189)
                     /locus_tag="PKM80_05305"
     CDS             complement(329..1189)
                     /locus_tag="PKM80_05305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV93363.1"
                     /translation="MRNRNKTSIPILIVLMVISISIGCQTKSSPIETSKEPLMSQKEN
                     TTTTTKVEMETTYGTIELELWNDLTPKTVQNFIKLASSGYYDGLYFHRVIPEFMIQGG
                     CPNTRDKDRSNDGRGGPGYTFEDECYKNGNEITGKIDTNQKAEAVWTKIIVPHIQTNR
                     TPNPDIATIAKECQEVRNFDPIMKHDLQFYQEKTGIKDKIYIQELIAPVNYGTICMAN
                     SGANTNGSQFFIVTKKDGAHWLDGKHTVFGKVTKGMEVVHKIENLPRDKNDNPNPENQ
                     AFITRISFPK"
     gene            complement(1206..1955)
                     /locus_tag="PKM80_05310"
     CDS             complement(1206..1955)
                     /locus_tag="PKM80_05310"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNV93364.1"
                     /translation="MKIIALSDSHYKFHCTDKADRENASIFVSFLNQCLGKYDLMVLS
                     GDIFDFWLEGKYTIVKQYFPVLCALNAIKEKGTQIIYISGNHDFWFGDFLSEYIGCKI
                     YPDGFTLNTDGKKIRFEHGDTRTLNDLRYQIYRKIIRWKVVRKIAASLHPDLALNIGM
                     LFSRSSREHTDNPALVERKSFGLKNYAKRLIEHNRADIVVLGHSHKPALEKIGNGYYV
                     NCGDWIKHYSYVEIDAGNPTLHKYNKESNQS"
     gene            complement(1952..>2411)
                     /locus_tag="PKM80_05315"
     CDS             complement(1952..>2411)
                     /locus_tag="PKM80_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNV93365.1"
                     /translation="SDPKLFDFPFLYLTGHGNIKFSEREVENLRTWMLRGGFLYADDD
                     YGMDESFRREIKRIFPDRELMELDPSFPLFNCFFDFSSGLPKIHLHDGKPPQAYGVFD
                     DNGRLLCLYTYETNISDGWADPETHNDPPEIRDKALKFGVNILYYVINKK"
BASE COUNT          694 a          445 c          439 g          833 t
ORIGIN      
        1 agaagcaacg ctcagatgct tcagctttga gcgttgcatt atgaacaata gaggctgttc
       61 cgcttaagcg aatataaccg gtttgtcctt cttcataaag aggaatgcag acctctatat
      121 ttttattatt ttgaatttgg ctaaccttag aatccccggt aaaagtagta acaaaaaatc
      181 ggtcgtcata gataaagagg acaacgggtc taactctggc tttattttta tctacagtag
      241 ccagatatac atattggttt tgttttatcc aagccattat ttcttgttgc agagcggatt
      301 cattggtgcg tgacataatt atctcatttt atttagggaa ggatattcgg gttataaatg
      361 cctgattttc cgggtttgga ttatcgtttt tatcccgggg taaattttct attttatgaa
      421 ctacttccat acctttagtc acttttccga aaacggtatg tttgccgtcc agccagtgag
      481 ctccatcttt tttagtaacg ataaagaatt ggctgccatt agtattggct ccagaatttg
      541 ccatacagat tgttccgtaa ttaacgggag cgattaattc ctgaatgtat attttatctt
      601 taattccggt tttttcctgg tagaattgaa ggtcgtgttt cataatagga tcgaagttac
      661 gaacttcctg acattcttta gcgatagtag caatatcagg attaggagta cggttagttt
      721 gaatatgggg aacgataatt ttggtccaga cagcttctgc tttttggttg gtatcaattt
      781 tgccagtaat ttcattcccg tttttatagc attcatcttc gaaagtatat ccaggaccgc
      841 cgcgtccatc gttgctacga tctttatctc tggtattagg gcatccacct tgaatcataa
      901 attccggaat aacgcgatgg aaatataaac catcataata accggaagag gcaagtttga
      961 tgaaattttg gacagttttg ggggtaagat cgttccaaag ttccagttca attgttccgt
     1021 aagtggtttc catttccact tttgtagttg tagtggtatt ttctttttga ctcattaatg
     1081 gctcctttga tgtttctata ggcgaagatt ttgtttggca tccgatactg atagagatca
     1141 ccattaaaac tatcagaatc gggatagagg tcttgtttcg atttcgcata ttatactcct
     1201 tttggttatg attgattgct ttctttatta tacttatgca aggttggatt tcctgcatca
     1261 atttccacat aagaatagtg ctttatccaa tcaccgcaat ttacataata accatttcct
     1321 attttttcca aagcaggttt atgactatgt cccaatacta ctatatcggc gcggttgtgt
     1381 tcaattaacc ttttagcata atttttcaat ccaaagcttt tgcgttcaac aagagcagga
     1441 ttatctgtat gttctctgct acttctggaa aagagcattc ctatatttaa agccaaatcg
     1501 gggtgtaatg atgcagcaat tttacggact actttccagc ggataatttt tcgatatatc
     1561 tgatagcgca gatcatttaa agtgcgtgtg tcgccatgct caaaacgaat ttttttacca
     1621 tctgtattta aagtaaagcc atcgggatat atcttgcagc caatatattc agataaaaaa
     1681 tctccaaacc agaaatcgtg atttccactt atataaataa tctgtgttcc cttttcttta
     1741 attgcattta gagcgcatag aacaggaaaa tactgtttta caatagtata tttgccttcc
     1801 aaccaaaaat cgaaaatatc tccagaaagc accattaaat cgtatttacc cagacattga
     1861 ttcaagaagc ttacgaaaat tgaggcattt tccctgtctg ccttatctgt gcagtgaaac
     1921 ttataatgac tatcggatag cgctattatc ttcatttttt atttatcaca tagtaaagaa
     1981 tatttactcc gaatttaagt gctttatctc ttatttcggg agggtcgtta tgcgtttcag
     2041 gatctgccca accatcactg atattagttt cgtaagtata taaacaaagc aggcgtccat
     2101 tatcatcaaa aaccccgtat gcttgaggag gtttaccatc gtgtaaatga attttgggaa
     2161 ggccactact gaaatcgaaa aagcagttaa aaagtggaaa agaaggatct aattccatca
     2221 gttctctatc tgggaaaatg cgttttattt ctcttctaaa agattcatcc attccgtaat
     2281 catcatcagc atataaaaaa ccacctctca gcatccaggt tctaaggttt tctacttcgc
     2341 gttcgctgaa tttgatgttt ccatggcctg ttaaatacaa aaaaggaaaa tcaaaaagct
     2401 taggatcgga t
//
LOCUS       DAORHB010000178         2386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_54319_length_2386_cov_3.280137, whole genome shotgun sequence.
ACCESSION   DAORHB010000178 DAORHB010000000
VERSION     DAORHB010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54319_length_2386_cov_3.280137"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKM80_05320"
     CDS             <1..553
                     /locus_tag="PKM80_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93366.1"
                     /translation="ITPITGLTVGGSYMYNTVGRDKDLNGEVTNPSYEKQQLMDIYTC
                     LAYGPVNLISECIIKKVDYPNLSDSNNTKEYTAKGISIFPILNLKDFVKYDLQLVGGY
                     DIWDESDRIETDKARNKVIASTIGVNYNFMPDETYTPVMQLQLNYIDKNYDEDKSHSD
                     YANGRRDSSQIILQLKWKFANIL"
     gene            572..1390
                     /locus_tag="PKM80_05325"
     CDS             572..1390
                     /locus_tag="PKM80_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV93367.1"
                     /translation="MKSIIKSVAPMLMMLLAFSLNAETKNQITCTGSTTVLPIAQATA
                     EVFMNNHAEINISVRGGGSGVGIASLQNKTTDICNSSRPLKSKEISTAKAKGINPIPY
                     AIANDAITIVVHNSNLLNNLSIKQIKDIYTGNIINWKELGGANVPIVVISRDVASGTY
                     EVFNDKVLAGAKVIGSAQLLASNNAVISAVSNTPGAIGYVGLGYLNDNAKLLSVNKVT
                     PSELTVKNNSYPLSRKLYMYTNGKAKGIVANYIGFIQSDRGQKIVVEQGFINIK"
     gene            1459..2331
                     /gene="pstC"
                     /locus_tag="PKM80_05330"
     CDS             1459..2331
                     /gene="pstC"
                     /locus_tag="PKM80_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNV93368.1"
                     /translation="MNKKYSEKAFQVLTYSTSLFTIIALLAIIYSLFKEGLPLFNKVP
                     IFDFLFGCNWYPTNVEPEFGAWTLIVGSLSVTIGALLIGIPLGLGSAIFISEVAGNCL
                     KEIVKPIIELLAGIPSVVYGLFGMSFLAPQIRNWLNLDTGLNIFSASVILGIMIVPIV
                     ASMSEDALSSVPKSLREAALALGSTKMECIFLVIIPAAKRGILGSIMLGVGRAIGETM
                     VVLMVAGGSAQIPQGVFSSVRPLTSTIAAEMGETVIGSLHYQSLYALAILLFLITFFS
                     NLITEFFILKRERK"
BASE COUNT          791 a          375 c          465 g          755 t
ORIGIN      
        1 tattaccccc ataactggtt taacagttgg tggttcctat atgtataaca cagtaggaag
       61 ggacaaggat ttgaatggag aagtcacaaa tccatcttat gaaaagcaac agctaatgga
      121 tatatatacc tgtttggcat atggccccgt aaatctgatc agtgaatgta tcataaaaaa
      181 ggttgattat cctaatcttt ctgatagcaa taatactaaa gaatatacag ccaaaggaat
      241 atcaatattc cctatactaa atttgaagga ttttgtgaaa tacgatcttc agctggttgg
      301 tggatatgat atatgggatg aatcggatag aattgaaact gacaaagcaa gaaataaagt
      361 aatcgcttct acaattggtg ttaattataa ttttatgcca gatgaaacat atacgcctgt
      421 tatgcaatta caattaaatt atattgataa aaactacgat gaagataaat ctcacagcga
      481 ctatgcaaat ggtagaaggg attcttcaca aatcatttta caactaaaat ggaaatttgc
      541 caacatttta taagcataaa ggagctaaaa aatgaagtca ataatcaagt ctgtcgctcc
      601 aatgttaatg atgttacttg catttagttt aaatgcagaa acgaaaaatc aaataacttg
      661 cacaggttca actacggttt tgccaattgc tcaagcaact gcagaggtct ttatgaataa
      721 tcacgcagaa atcaatattt cagttagagg aggtggctct ggagtaggaa ttgcttcttt
      781 gcaaaataaa actaccgata tatgcaattc cagtaggcca ctaaaatcca aggaaatatc
      841 aaccgctaaa gcaaaaggga taaacccaat tccttatgct atcgccaacg acgctattac
      901 cattgttgtg cacaatagta atttgttgaa caacctatca attaagcaaa taaaagatat
      961 atatacaggt aacattataa attggaagga gttaggagga gcaaatgtac ccatagtagt
     1021 tatttctcgc gatgttgctt ctgggactta tgaagtattt aacgacaaag tattagcagg
     1081 agcaaaagta attggtagtg cacaattact ggcttccaat aatgcagtta tcagtgcagt
     1141 aagtaacacc cctggagcta ttggatatgt gggtctgggc tatttaaatg ataatgctaa
     1201 actacttagt gtaaataaag ttacaccttc agaacttact gttaaaaata acagttatcc
     1261 tctttcccga aaactgtata tgtacactaa tggcaaagca aaaggcattg ttgctaacta
     1321 tatcggtttt attcaaagtg atcgtggaca aaagattgtt gtagaacaag gttttataaa
     1381 tatcaagtaa agtgaacgaa agaactgtgg gtagaggtta accctctgcc cttcattaat
     1441 attctgatga ggtctatgat gaataaaaaa tatagtgaaa aagcatttca agttcttact
     1501 tacagtacat ctttatttac tattatagct ctcttggcta taatatacag cttgtttaaa
     1561 gaaggcttac cactttttaa caaggttcct atttttgatt ttctgttcgg ttgcaattgg
     1621 tatccaacaa atgttgagcc tgaatttggt gcctggacac taatcgttgg ttctttatcg
     1681 gtaacgattg gtgctttact tataggaata cctttaggtt tagggagtgc aatatttata
     1741 tctgaagtag cgggaaactg tttgaaggaa atagtcaaac ccataataga attattggca
     1801 gggatccctt ctgtggtata cggacttttt ggaatgtcct ttctagctcc gcaaattaga
     1861 aattggttaa atttggatac gggtttaaat atattttctg cttctgtaat tttaggaatt
     1921 atgattgttc ctatagtagc cagtatgagt gaagatgctc tttcaagtgt acccaaatct
     1981 ttgcgggaag cggctttggc attgggatct acaaaaatgg agtgtatttt tcttgttatt
     2041 attccagctg ctaaaagagg tatattggga agtattatgc ttggtgtggg tagagccatt
     2101 ggagaaacaa tggtcgtttt aatggttgct ggaggtagcg ctcaaattcc tcaaggggtc
     2161 ttttcttctg tgcgcccgtt aacttctaca attgctgctg aaatgggaga gaccgttatc
     2221 ggaagcttac attatcaatc gctttatgca ttagcaattc tgttatttct aataactttc
     2281 ttttccaatt tgataacaga attcttcatt ctaaaaaggg agcggaaatg atcagaagga
     2341 aaattgttaa taacatagga ctcagcttac taacacttct gctatg
//
LOCUS       DAORHB010000179         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_54895_length_2369_cov_7.031115, whole genome shotgun sequence.
ACCESSION   DAORHB010000179 DAORHB010000000
VERSION     DAORHB010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54895_length_2369_cov_7.031115"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /gene="cas1"
                     /locus_tag="PKM80_05335"
     CDS             <1..896
                     /gene="cas1"
                     /locus_tag="PKM80_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNV93369.1"
                     /translation="DYNIDIVFLDKYGNPYGRIWFPKIGSTVLIRRRQLEMLSDSVGL
                     QFTKDWVAIKIMNQYRFVQRLLSKRDCDKSIFQNRMQNMQEAAICIMQAEGNLEELSG
                     SFMGWEGSASKNYFSILAELIPDAYKFAGRSSRPAKDAFNAMLNYGYGMLYSKVERAL
                     IIAGLDPYVGILHSDNYNKKSFVFDFIEPYRILVDEPVFYIFSRHKFSPEFIEPVHQG
                     VLLSAAGKKFLAPLLLEHFDEIIRYRNKNRKRIEMLKTDAHAFANYLIDKRENYLETS
                     INRKLQNFLNSNFEDNGGEEC"
     gene            890..1180
                     /gene="cas2"
                     /locus_tag="PKM80_05340"
     CDS             890..1180
                     /gene="cas2"
                     /locus_tag="PKM80_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNV93370.1"
                     /translation="MLTWVMYDIVKDKIRNKVSKCCEQAGIYRVQYSVFLGEMSRTKC
                     KELSCQIEDLINPEIDRVYIFPMCNEDFKSCQLLGQAFDPELITNEIKAFFL"
     gene            1177..1767
                     /gene="cas4"
                     /locus_tag="PKM80_05345"
     CDS             1177..1767
                     /gene="cas4"
                     /locus_tag="PKM80_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNV93371.1"
                     /translation="MSDFQFITPSEVMEYLFCPRFVYYMNTMKIEQHEHRRTLVNKGR
                     DIHKLKMVQNKDYLRKKAGAIDKLTDVYLSSEKLKLVGKVDEVLFLVDGSAAPLDYKY
                     AFWENKIYKTLKYQQILYSLLIMENFQVPVYKAFIVYTRSQNHLEELAITNRMLDKAK
                     LILDDIFKIINMEIYPKPAKAKRKCLDCTYRNLCSA"
BASE COUNT          758 a          367 c          456 g          788 t
ORIGIN      
        1 tggattataa tatagacatc gtttttctgg ataaatatgg caatccttat ggcagaattt
       61 ggtttccgaa gattggcagc actgttttaa ttcggagaag gcagctggaa atgttatctg
      121 atagtgtagg tttacaattt actaaagatt gggttgccat taaaataatg aatcagtatc
      181 gttttgtgca gcgtttactt tccaagcggg attgtgataa aagtatcttt caaaatcgga
      241 tgcaaaatat gcaggaagca gctatttgta taatgcaggc agaaggtaat ttagaagaac
      301 tttcgggttc ttttatgggt tgggaaggaa gtgcgtccaa aaattacttt tctattctgg
      361 cagagctcat tccagatgct tataaatttg cgggcagaag ttcgcgtcct gccaaagatg
      421 ctttcaatgc tatgttgaat tatggctatg gtatgcttta ttccaaagtg gagcgagctt
      481 taattattgc tggtcttgat ccctatgttg gtattttaca cagtgataac tataacaaga
      541 agtcctttgt ctttgatttt atagagccgt atcgtatttt ggtggatgag ccggtattct
      601 atattttttc ccggcataaa ttttctcctg agtttatcga accggttcat caaggtgttt
      661 tacttagcgc tgcaggcaaa aaattcctgg ctcctttgtt gttagaacat tttgatgaga
      721 ttattcggta tcgcaataaa aaccgtaaac gcattgaaat gctaaaaact gatgctcacg
      781 cctttgccaa ttacttaatt gataaaaggg aaaactatct ggaaacatct ataaacagga
      841 aattacagaa cttcctaaat agcaattttg aggataatgg aggtgaagaa tgttaacttg
      901 ggttatgtat gatatcgtta aagacaaaat tcgaaataaa gtttccaagt gctgtgaaca
      961 agccggaatt tatagagttc aatactctgt ttttttggga gaaatgagcc gcactaaatg
     1021 caaagaacta agctgtcaga tagaggactt aatcaatccc gaaattgatc gagtttatat
     1081 ttttccgatg tgcaatgagg attttaaaag ttgtcaattg cttgggcaag catttgatcc
     1141 tgaactcatt acgaatgaaa taaaggcatt tttcctatga gcgattttca gtttatcact
     1201 ccttccgaag tgatggaata tctcttctgt ccaagatttg tttattatat gaacacaatg
     1261 aaaattgaac aacacgaaca cagaagaact ctcgtaaata aaggcagaga tattcataaa
     1321 ctgaaaatgg tacaaaataa ggattattta cgcaaaaaag ccggtgccat tgataaacta
     1381 acggatgtat atctttcttc cgaaaaactt aaactggtag gaaaggtcga tgaggttctt
     1441 tttcttgtcg acggcagtgc cgcgcctctg gattataaat atgccttttg ggaaaacaag
     1501 atctataaaa cccttaaata tcaacagatt ctctattccc ttcttataat ggaaaatttt
     1561 caggttcctg tttataaagc atttattgtc tatacgagat ctcaaaacca ccttgaagaa
     1621 ttggctatta ccaatagaat gctggataaa gctaaattga tccttgacga tattttcaag
     1681 atcattaata tggaaatata ccctaaacca gcaaaggcaa aaagaaaatg cttggactgc
     1741 acctatcgga atttgtgttc tgcctgacta aatgagagaa aaacagtttt ttgcttttct
     1801 gaataaactc tttattatat agtgtgtaac aggatattac aagacacttg agattcctga
     1861 tttttatcgg atatgataac aaatatagct agttactata ttatagcttg tcattccgag
     1921 cttgatgcag aatctttatt attcctgcta ttgtcaggac tataagtgtt tttttttaag
     1981 agagatagta ataattgatt tatccttgct gatatatatg ctttgtactt gaatcctgag
     2041 aagaaattat tgttttttta gcgtgttttt gctcttttat atgtgtataa agtgtttgtg
     2101 cctgcccgaa agaacttttt ggctctttcg agaactggtt ttcctgacga aagagtgatt
     2161 ttaagattcc gaaaaaccag tttaatacta ttatttgtgt ggcacttatg acaaaagaag
     2221 gatttatatc ctactatcca ttaaaacaag gattgaaacc aatttcaata tgcatagaaa
     2281 agtggcaatt cataatttat atcctactat ccattaaaac aaggattgaa actgcaaaaa
     2341 ttagtaggat tggctaccgg tagaatagg
//
LOCUS       DAORHB010000180         2360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_55216_length_2360_cov_5.373536, whole genome shotgun sequence.
ACCESSION   DAORHB010000180 DAORHB010000000
VERSION     DAORHB010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55216_length_2360_cov_5.373536"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PKM80_05350"
     CDS             <1..1195
                     /locus_tag="PKM80_05350"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNV93372.1"
                     /translation="HGPQVVNQNITIEGLPFLESVRQLRLDIGYENTLNILLTYVPYI
                     KSAGELKTKPSQHSAYKLREIGIQPDVLLCRSEKPFEEEIYSKIALFTNVKRENVINA
                     IDVDCVYEIPLNFQKAKLPEIICQHFKLEQRPIVMNEWLHFLHNRENAEEVVTIAVCG
                     KYVQHQDAYKSIGEALIHAGASLMKRVKIIWIDSEHNFLEEEIATMLNGVQGILVPGG
                     FGVRGIEGKIVFTKYARENNIPYLGICLGMHVAVIECARDLCNLVDANSTEFNEETLA
                     PVIHLIKDQVYQKRIGGSMRLGAYDCHLDKGSKAYQAYQTETISERHRHRYEFNNDYR
                     EIIQKAGLKISGLSPDNYLVEIVEYPDLDFFIAVQFHPEFKSRPTNPHPLFREFIKAA
                     SLKKP"
     gene            1889..>2360
                     /locus_tag="PKM80_05355"
     CDS             1889..>2360
                     /locus_tag="PKM80_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV93373.1"
                     /translation="MNNKRETLALLLLLLSIFLLTGCKKSDKQNGAITQSNMEQSPYS
                     TGLFTIRYLPVWTHQAEFAGVYMAQKKGFYNDKGLNVLIQTGGPHYPPYDNLQDGHSD
                     IVQMSLLTAIKRDSEKDNLVNLAQLMQRTYLMLVGKKSRGINSIADFNGKRIGLW"
BASE COUNT          811 a          354 c          451 g          744 t
ORIGIN      
        1 gcacgggccg caagtagtca accaaaacat cactattgaa ggtttgccat ttttggaatc
       61 agtccgccaa ttacgtttag atattggtta tgaaaatacg cttaatattc tattaactta
      121 tgtaccatat ataaaatcag ccggagaatt aaaaaccaaa ccttctcaac atagtgctta
      181 taaacttagg gaaataggta ttcaaccgga tgttttactt tgcagaagcg agaaaccttt
      241 tgaagaagag atttatagta aaatagccct gtttaccaat gtgaaacgag agaatgtgat
      301 taatgcaatt gatgtagatt gtgtttatga aattcccctt aatttccaaa aggcaaaact
      361 ccctgaaatt atttgccagc atttcaaact tgaacaaaga ccaattgtta tgaatgaatg
      421 gttgcatttt ttgcataatc gcgaaaatgc tgaggaagtt gttactatcg ctgtctgtgg
      481 aaaatatgtg caacaccagg atgcctataa aagtattgga gaagcgttaa ttcacgcagg
      541 tgcatctttg atgaaaagag taaaaattat atggattgat agcgaacaca attttttgga
      601 agaagaaatt gcaactatgc tcaatggtgt tcaaggtatt ttagttcctg gtggatttgg
      661 tgttagaggt atagaaggga aaattgtttt taccaaatat gcccgagaaa ataatatacc
      721 ctatctgggt atctgtctgg gaatgcatgt tgcagtaatt gaatgtgccc gagatctttg
      781 taatcttgta gatgctaata gtacagaatt taacgaagaa actttagccc cagtaattca
      841 tcttattaaa gatcaagttt accaaaaaag aattggcgga tctatgcgct tgggagctta
      901 tgattgccat ttggataaag gtagtaaagc ttatcaagcg tatcaaacag aaaccattag
      961 tgaaagacat agacaccgct acgaatttaa taatgattat cgcgaaataa ttcaaaaggc
     1021 aggattaaag attagtggat tatcacctga taattattta gtggaaatag tagaatatcc
     1081 ggatttggat ttctttatag cagtgcagtt tcatccagaa tttaagagca gacccactaa
     1141 tccacatcca ttatttagag aatttatcaa agccgcgtca cttaagaagc cataaacgga
     1201 aattgtctac tcaaacaaaa agtacagcgt acaataaact ggaatattat gttcttattt
     1261 gttgcggttt cttccattat aacaaaaaca tagcgataga tacttattat tttaaagcac
     1321 caacataata agatgttatt ttagtaaagc tgctatgtag ataatgaatt ttctgtaacc
     1381 ctcccctcgc aacacaggag ggttacagtt tgcatgctaa tgatgatgag atacattata
     1441 tctaattagc tagttgtgct ttttacagat gatctgtcga atattttcac aataaagtcc
     1501 tatataaaag aaatgtaagc gctgtagaaa tttatcaaca cgaaaagaca ggatatttgt
     1561 tcgattctgt ttgacaggat atttatttat attattgtta atatgtagta agaaagatat
     1621 gggatttata atagcagatt ttctgcctgt gcaagaaaaa atcgaatttc cttgtttagg
     1681 tatgtacatt ttcttgcact aacgaatatg aatctgttat atttattgag agaagatagc
     1741 agggattgtt acttcttaga gagattcttc gtaactttgg gtaggatgtt gttatgccca
     1801 gtattgtatt atagctataa tggccatagg gagtttcatg ggatgatata cattattgcg
     1861 ttacatgtta agagaggaat agatctatat gaataataag agggaaacgc tagctttact
     1921 actgctttta ttatcaatct tcttattaac gggatgcaaa aaatccgata aacaaaacgg
     1981 tgctataact caatccaata tggaacaatc accatatagt actggtttat tcactattcg
     2041 ctatcttcct gtatggactc atcaagcaga atttgccggt gtttatatgg ctcaaaaaaa
     2101 aggattttat aacgataaag ggttgaatgt actaatccaa accggtggtc cccactatcc
     2161 tccttacgac aatttacagg atggacatag tgatattgtg caaatgtcac tattaactgc
     2221 tataaaaaga gattctgaaa aagataattt agttaatctt gcgcaattaa tgcagcgaac
     2281 ctatctgatg ttggtaggta aaaaatccag aggaataaac tcgatagctg attttaatgg
     2341 aaagagaata ggtttgtgga
//
LOCUS       DAORHB010000181         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_55823_length_2344_cov_4.744867, whole genome shotgun sequence.
ACCESSION   DAORHB010000181 DAORHB010000000
VERSION     DAORHB010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55823_length_2344_cov_4.744867"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKM80_05360"
     CDS             <1..606
                     /locus_tag="PKM80_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNV93374.1"
                     /translation="VFSGMNDVAVLGNGTIILATLGANRTINLRRLNSDGTFTTIDTW
                     QLNFLDPQTQIHPKSKICLQPRHQNALEVTFMLFAEGENGEPDYYQLYRADASVPLAI
                     EDPSVPAIEPVMFYAYPNPVHTNLKLQVKVPNQNTHKIEIYNLKGQKIATLKGNSAKN
                     EIAEYNWNGCDAKGKSVSAGIYLVRLTVDGKVVQSKRICKY"
     gene            complement(1164..2108)
                     /locus_tag="PKM80_05365"
     CDS             complement(1164..2108)
                     /locus_tag="PKM80_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV93375.1"
                     /translation="MAYLYVVLIFAILVLTFISRGLIIVRQASVVIVERLGKYYRTLD
                     SGIHIIIPIFDKTRPIHWRYNKIDYRGNVVVVNKIEDRIDLRENVYDFPRQNVITSDN
                     VSININALLYFQITDPYKAVYEIGNLPEAIEKLTQTSLRNVIGELTLQDTLTSRDAIN
                     AKLRDILDEATDKWGVKVNRVEMQEILPPEEIRTAMEKEMRAERDKRAKILQADGERE
                     YQIRVADGEKQARIARAEGESQAKKLVAEGERQAIISIAEAVKNTGTDPAQFQIALRY
                     IEAFREIVKQGDKTVVLPYESSALLGSVKTLGEIFQKV"
BASE COUNT          728 a          472 c          413 g          731 t
ORIGIN      
        1 gttttttcag gaatgaacga tgtagccgtt ttaggaaatg gaactattat ccttgccact
       61 ttgggagcaa atcggacgat caatcttcgg agattaaata gcgatgggac ttttactacc
      121 atagatactt ggcaattgaa tttcctggat ccgcaaaccc aaattcatcc taaaagcaaa
      181 atatgcttgc aaccccgtca ccaaaacgct ctggaagtaa cctttatgct atttgcggaa
      241 ggagagaacg gagagccgga ttattatcag ctttaccgag ctgatgcatc cgtgcctctt
      301 gccatcgaag atccttctgt tcctgccata gaacctgtta tgttctatgc ctatccgaac
      361 cctgttcata ccaatttgaa acttcaagtg aaggttccca atcagaatac tcataaaatc
      421 gagatttata acctgaaagg acaaaaaatt gctactctaa agggaaattc ggctaaaaac
      481 gaaattgccg aatacaactg gaatggctgt gacgctaaag gtaaaagtgt ttctgccgga
      541 atttatcttg tccgcttaac tgttgatggt aaggttgtac aaagtaagcg aatctgtaaa
      601 tattgatcta taaaggaggg ttgacttttc tggtcaaccc tccaacttaa gaaaagccat
      661 ttgcaccttg ataacccatt ctctgtagat atcttttgta gggcagagaa ctaattacca
      721 ctataataca ttatctatac acatattacc agtatcggag tacaataaga taaaaaataa
      781 atattccttc cctgtataaa atagccccat caggacgaca aatactaaca ccgaattgta
      841 tgttacaata acttcaaaac aaaaatactc cttccttagt tacctgaagt agaactaccg
      901 tcctgcttct ggggaaaatc cccaagaggc tgcaccacag ctctaccata gatccctaag
      961 gaagatatag aaatactcct aacattaaag aagcaatatc gttatatggc tattgcaata
     1021 ttaggattag aatttactat atcttcctta tagccttcgg aaaaatattg gcgtaaccaa
     1081 gatccgcttc acggttacga taaagcgtaa attttctgtt acctagggta agacttaata
     1141 acttgtaatt agaaaatagt tatttatacc ttttgaaaaa tctcacccag ggttttcacg
     1201 ctgcctaaca gggctgatga ttcatagggt aaaacgactg ttttatctcc ttgtttgaca
     1261 atctctctga atgcttcaat atatctaaga gcaatctgaa actgagctgg atcggttcct
     1321 gtatttttta cagcttcagc tatggatata attgcttgtc tttcaccttc ggcaaccagt
     1381 ttttttgctt gtgattcacc ctctgcacgc gctatgcgag cttgtttttc tccgtctgca
     1441 acccgaattt gatattctcg ttccccatct gcttgtaata ttttggcgcg tttatcgcgt
     1501 tcggcgcgca tttctttttc catagccgtt ctaatttctt ctggaggtaa aatttcttgc
     1561 atttccaccc ggtttacttt tacgccccat ttatcggtag cttcatctaa aatatcacgc
     1621 aatttagcat taatagcatc gcgcgaagtt aaagtatctt gcaaggttaa ttctccgata
     1681 acattacgca gtgaggtctg agtaagtttt tcaatcgctt caggtaaatt gcctatttca
     1741 taaacagcct tataaggatc ggttatttga aaataaagca aagcattaat attgatggaa
     1801 acattatcac tggtgataac attttgccgg ggaaaatcat aaacattctc ccttaggtca
     1861 attctatcct ctattttatt tacaacaaca acatttccgc gatagtcaat tttgttataa
     1921 cgccaatgaa ttggacgggt tttatcaaaa ataggaatga taatgtgaat cccggaatcc
     1981 aaagtccgat aatattttcc taagcgttct acaattacga cactcgcctg acgcacaatt
     2041 atcagtccac gagaaatgaa agtaagaact aagattgcaa aaatgagtac cacatatagg
     2101 taagccatta ttacctccct aactattttt tttccttatt tccccacttt gtttttttgg
     2161 caagaaaaaa ctgaaaaaga gtaggtcaaa gtgtgggagt tcaaagttca aagtgcggga
     2221 gttcaaagtt caaagttatg ctttttgtgt tttcagtgtt ttcggtgatt tcggtggcta
     2281 aaaaaattta cttgacttaa gttgccttcc caaaaataat aaaaaaaaga aatcaagtga
     2341 ggta
//
LOCUS       DAORHB010000182         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_56752_length_2320_cov_3.787638, whole genome shotgun sequence.
ACCESSION   DAORHB010000182 DAORHB010000000
VERSION     DAORHB010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56752_length_2320_cov_3.787638"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /gene="aspS"
                     /locus_tag="PKM80_05370"
     CDS             complement(<1..1681)
                     /gene="aspS"
                     /locus_tag="PKM80_05370"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNV93376.1"
                     /translation="MLDNLGNLTRSHYCGDLNTDHIGQNVTVMGWVNKRRDLGGLIFI
                     DLRDVKGILQIVIRPEQEEIFAKAEKVRNEYVIAVCGIINARSEQNINPSIPTGKIEL
                     VAEDFYILNDAQPLPVQFSEAAMAEEDLRLTYRYLDLRRPKLQNIIITRHRIMQIIRD
                     FLNNEGFYEIETPILMKSTPEGARDYLVPSRVQPGKFYALPQSPQMFKQLLMIAGFDK
                     YYQIARCFRDEDLRADRQPEFTQLDIELSFVTQAQIFDLLERLMYKLFKGILDVSLSI
                     PFPIIPYAEAMENYGCDKPDIRFGMKLNDISTIVANSSFNVFSSALQNGGVVKAIAIP
                     GAANFSRKQQDELIELAKHLGGKGIAFAKVTENGLDAGISKFLSLEETEAIIDVTGAV
                     TNDLIAIAADNYEMVCKVLSGIRNYLAEKLGLIPEDNYAFCWITDFPLFAKNAETGAW
                     EPAHHMFTLPKEEHIPYLDLPEKIGEITGQLYDLVCNGVELSSGSIRCHRYDIQKKIF
                     DILGFSNKELQDRFGFFLEALKYGTPPHGGIAPGLDRLVMIITKAESIRDVI"
     gene            complement(1706..>2320)
                     /locus_tag="PKM80_05375"
     CDS             complement(1706..>2320)
                     /locus_tag="PKM80_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV93377.1"
                     /translation="INSRSWNILGNAFFTGKNKVTMLDDAREVVGAALIYNGYDLNDS
                     SVMALAAAEKTLAQWDKNITQFDSDSYKNEVPDGTTWLAQAYNGDALQVIDNSPDLGF
                     ILPVEGASLWMDNIAMLKSSNNKELAYKFINFLLDADIGKRNTEYCLYATPNKAAGNL
                     LPETLKNNTLIYPGDEYLNKCYMINALGEDVKKIDKLFEAIKLN"
BASE COUNT          711 a          474 c          370 g          765 t
ORIGIN      
        1 caataacatc ccggatggat tctgctttag ttataatcat aactaatcta tccaaacctg
       61 gagcaatacc tccatgaggt ggtgttccat attttagcgc ttccaaaaag aatccaaaac
      121 gatcttgcaa ttctttattg ctaaatccga ggatatcaaa aattttcttt tgaatatcat
      181 agcgatgaca tctgatgcta ccactggata gttctactcc attacaaaca agatcataaa
      241 gctgacctgt aatttcgcca attttttcag gtagatcaag atagggaata tgttcttctt
      301 ttggtaaagt aaacatatga tgagcaggtt cccaggcacc ggtttctgcg tttttggcaa
      361 acagcgggaa atctgttatc cagcaaaatg catagttatc ttcaggaatc agacctaatt
      421 tttcagctaa gtaattacga atgccagata ataccttgca taccatttca taattatctg
      481 ctgcaattgc aataaggtca ttagttactg ctccagtaac atcaattatt gcttcagttt
      541 cctctaagct aagaaattta ctgatacccg catccaaacc attttcagta actttggcaa
      601 aagcaattcc tttacctccc aaatgtttag ccagttcgat aagttcatct tgctgttttc
      661 tactaaaatt agctgccccc ggaatagcaa ttgcttttac tacgccacca ttttgtagtg
      721 ctgaagaaaa aacattaaaa ctgctattag caacgatcgt agatatgtca ttcagtttca
      781 ttccaaaacg gatatcaggt ttgtcgcatc cataattctc catcgcttct gcataaggaa
      841 taatcggaaa aggaatgctt aagctaacat ctaaaatacc tttaaagagc ttatacatta
      901 agcgttccag taaatcaaaa atttgtgcct gcgtaacaaa actaagctca atatccagct
      961 gcgtaaattc tggttgccta tcggctctta aatcttcatc tctaaaacaa cgcgcaattt
     1021 gatagtattt atcaaaaccg gcaatcatta aaagctgctt aaacatctga ggagattgag
     1081 gcaaagcata aaatttccct ggctgaactc tactggggac aagataatct cttgcccctt
     1141 cgggagtgct tttcatcagg attggggttt ctatttcata aaaaccttcg ttatttaaaa
     1201 aatcccgtat tatttgcata atgcgatgtc gagtaataat gatattttgc agtttgggtc
     1261 ggcgcaaatc cagataacga tatgtgagcc gtagatcttc ttctgccata gctgcttcac
     1321 taaattgtac aggtaggggt tgcgcatcat ttaagatata aaaatcctct gctaccagct
     1381 caattttccc agtgggaata gaaggattga tgttttgttc actgcgtgca ttaattattc
     1441 cgcaaacagc tattacatat tcatttctaa ctttttctgc tttggcaaag atttcctctt
     1501 gttcaggacg aataacaatt tgtaaaatac ctttaacatc gcgtaaatca ataaatatca
     1561 aaccaccaag gtcacggcgt ttatttaccc aacccataac ggttacattc tgtccaatat
     1621 gatcagtgtt caaatccccg caataatggc tgcgagttaa atttcccaga ttatcgagca
     1681 tttttgtttc tttattctca ctttcttagt ttaattttat tgcctcaaag agtttatcta
     1741 ttttcttcac atcttcacca agagcattaa tcatataaca tttgtttaga tattcatctc
     1801 ctggatagat tagagtatta ttctttaatg tctcgggtag taaattgcca gctgctttat
     1861 tcggagtggc atatagacaa tactcagtat tccttttacc aatatcggca tctaagagga
     1921 aattgataaa tttataggca agttctttat tattactaga ttttaacatt gcgatattat
     1981 ccatccataa acttgctcct tccacaggta agataaaacc taaatcagga ctgttatcaa
     2041 ttacttgtaa agcatctcca ttatatgctt gagctaacca agtagttcca tctggaactt
     2101 catttttata gctatcagaa tcaaattgag tgatgttttt atcccattgg gcaagggttt
     2161 tttcggcagc tgctaatgcc attacacttg aatcattaag atcgtatccg ttataaatca
     2221 atgctgcacc tacaacttca cgcgcatcat ccaacatcgt aactttgttt tttccggtaa
     2281 aaaaagcatt gcccagaata ttccaacttc gggaattaat
//
LOCUS       DAORHB010000183         2308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_57195_length_2308_cov_4.349756, whole genome shotgun sequence.
ACCESSION   DAORHB010000183 DAORHB010000000
VERSION     DAORHB010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57195_length_2308_cov_4.349756"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..52
                     /gene="ssrA"
                     /locus_tag="PKM80_05380"
     tmRNA           <1..52
                     /gene="ssrA"
                     /locus_tag="PKM80_05380"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            143..412
                     /gene="rpsO"
                     /locus_tag="PKM80_05385"
     CDS             143..412
                     /gene="rpsO"
                     /locus_tag="PKM80_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNV93378.1"
                     /translation="MPLTPEAKQAIMAEFKLHESDTGSPEVQVALLTQRIKDITEHLK
                     EHPKDFSTRRGLLKLIGQRRRLLDYLKKKDITRYRNLIKALGIRK"
     gene            717..>2308
                     /gene="pnp"
                     /locus_tag="PKM80_05390"
     CDS             717..>2308
                     /gene="pnp"
                     /locus_tag="PKM80_05390"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNV93379.1"
                     /translation="MHNFNITSRETDFCGRKLIVETGKMAKQANGSVFIQYGESAILV
                     TATMSKEPVENQDFFPLTVDYIEKMYAAGKIPGGFFKRESKPSTDATLMARVIDRSIR
                     PLFPEGFRNQVHIVVTVLSFDGVTDPSTMGIFGASLALSISDIPFNGPIAGATVGYID
                     DQFVVNPHFDEIESKSKLNLTVAGSDDSIVMIESAASVLSEETMLEAVFIGHNEIKKL
                     CSLQKDFISACGKEKVEVVIDSIPEEIMNRVENAFGLKIAEAAVIFGKQERQDAFDAA
                     EAEMVEMFTAIEKDAWEEKEKYYQKAFEELIRRYVRDSILKKHHRVDSRGTDDLRDIT
                     CEIDILPRVHGSALFTRGETQSLGTLTLGGGIDEQMIDGLENEYKKSFFLHYNFPPYS
                     VGEAGKMGPTGRRELGHGNLAERALKAVLPDKDVFPYTIRVVADTLESNGSSSMASVC
                     SGCLALMAGGVPIKAPVAGIAMGLIMEDKDYVVLTDIMGLEDHLGDMDFKCAGTREGI
                     TAMQMDIKISGITKEIMQIALN"
BASE COUNT          747 a          389 c          494 g          678 t
ORIGIN      
        1 tttcgggtga tcggttttct ggacgcgggt tcgattcccg ccacctccac caaaaaagca
       61 aactcattaa cattgaaaaa tattactgga caaataggtc acctaccaaa gtatggtgct
      121 ataaaatcat ctggaggatg aaatgccgtt aacacctgag gctaaacaag ctatcatggc
      181 agagtttaaa ctccatgaat cagacacagg ctcgccggaa gttcaagttg ctttgttaac
      241 tcaaagaata aaagatatta cagagcatct aaaagaacac ccaaaggatt tttccactcg
      301 gcgtggcttg ttaaaactga tcgggcaacg caggcgttta cttgattatt taaaaaagaa
      361 agatatcact agatatcgaa atttaatcaa ggcgcttggc ataaggaaat aaactcttag
      421 tgtatctata gtttagcatt aatactgaac tttggatcgc tcaatgcaag agaaagtgaa
      481 ttttaggaat ttacctatta ttcactttcc ttcgctttat aatgggttga ctaaaaaaat
      541 gctctgtagt taaaatagaa agaagataat attatatttt aagcagttat cgaatgggaa
      601 aatgtcaacc ttaaataata taaagcggga gaaattagca ataagccaaa aggtttacaa
      661 agaagacctt ttcgcctatt gcttcttctc ccgccaaaat agaaaaagga gaagttatgc
      721 ataacttcaa tattacaagt cgagaaaccg atttttgcgg gcgcaaactg attgtagaaa
      781 ctggtaaaat ggcaaaacaa gctaatggca gtgtttttat ccaatatgga gaatcagcaa
      841 ttttagttac agctacaatg tctaaagaac ccgttgaaaa tcaggatttc tttcccttaa
      901 ccgtagacta cattgaaaag atgtatgcag ctgggaaaat tcctggcggt ttttttaaac
      961 gcgaatctaa gccctctact gatgccactt taatggcaag ggttatagat cgttcaattc
     1021 gtcctctatt tccagagggc tttcggaatc aagttcatat cgtagtaact gttctatcat
     1081 ttgatggagt aacagatcct tctacgatgg gtatttttgg tgcttcctta gcgttatcga
     1141 tttccgatat accttttaat ggtcctattg caggtgctac agttggttac attgatgatc
     1201 agtttgtagt aaacccccat tttgatgaga ttgaatcaaa atccaaactc aatctaaccg
     1261 ttgctggtag tgatgattct attgtgatga ttgaatcagc agcttctgtg cttagcgaag
     1321 aaacgatgtt agaagctgtc tttattggcc ataatgaaat taaaaagcta tgttctttac
     1381 aaaaagattt tatttctgct tgcggaaaag aaaaggtgga agttgttata gatagcattc
     1441 cagaagagat aatgaacagg gtggaaaatg cctttggtct aaaaatagct gaggcagccg
     1501 ttatttttgg taaacaagaa cgtcaggatg cctttgatgc agcagaagca gaaatggtag
     1561 aaatgtttac agcaatagaa aaagatgcct gggaagaaaa agagaaatat tatcaaaaag
     1621 catttgagga acttatccgt cgttatgttc gcgattctat actaaaaaaa catcatagag
     1681 tagatagtcg cggaactgat gacctccgtg atattacctg tgaaatagat atcttaccca
     1741 gagtgcatgg ttcagctttg ttcactcgtg gtgaaacgca atctttagga acccttacct
     1801 tgggtggtgg aattgatgaa caaatgatag atggcttgga aaacgaatac aaaaagagct
     1861 ttttcctgca ctataatttt ccgccctata gtgttggcga agcaggtaag atgggcccta
     1921 cggggcgtcg tgaactgggc catggtaatt tagcagaaag agcgctgaaa gctgttttac
     1981 ctgataaaga tgttttcccc tacactattc gtgtggtagc cgatactctg gaatctaatg
     2041 gttcatcctc aatggcttca gtttgtagtg gatgcttagc tttgatggct ggtggtgttc
     2101 ctataaaagc tccagttgca ggaattgcaa tgggattaat tatggaagat aaagactatg
     2161 tggttttaac cgatattatg ggtttggaag atcacttagg agatatggat ttcaagtgtg
     2221 ccggaactcg tgaaggtata actgctatgc agatggatat taaaatatct ggaatcacca
     2281 aagaaattat gcagatagca cttaataa
//
LOCUS       DAORHB010000184         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_58457_length_2276_cov_3.618640, whole genome shotgun sequence.
ACCESSION   DAORHB010000184 DAORHB010000000
VERSION     DAORHB010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58457_length_2276_cov_3.618640"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKM80_05395"
     CDS             <1..704
                     /locus_tag="PKM80_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV93380.1"
                     /translation="LEHLLKDIGSKNSLIVVDGIFSMEGDIANLPAICDLAEKYNSEV
                     MVDEAHSLGVLGNLGAGTTAHFGLTDKTAFIMGTFSKSLASVGGFIAADEALIHYLKH
                     KSRALIFSASLPPASTASVLAALKIMEEEPERIAKLWENTNYMLSEFKAMGYNTGDSC
                     TPVVPLHVGNMMTAFNMWRRLGEEGVFINPVVPPAVPPSSCLIRTSFMATHTQDQLDM
                     ALDKFRMIGKELNII"
     gene            766..>2276
                     /locus_tag="PKM80_05400"
     CDS             766..>2276
                     /locus_tag="PKM80_05400"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNV93381.1"
                     /translation="MASKSSVEYYSNIKEMSPIRAIAETLMNNHLVRKVDIREAYELA
                     IKQPGVSVTDLPMYPEFVKLHNLPKDAKIINDCHGNIIGRTAKARRFYNRVNPSLKNK
                     LESDFREAVWQMQHYPLIKAEAILGMDKDLMLKATFITTESDAANLYFWLLNFAPYES
                     LAQEYEASPKLPIQDIILVAFNEWTCNDSYYNNVGAPQLALVDEKHNVMVNLGMRYFG
                     ERKKGTLTLAWTSGIRIGMAACHGGIKEIDFGTCENPEYHKLGKRSIAFYGLSGTGKS
                     SHTNNADNAGTLPKGFSKVVLHDDAFQIDLENKQCRAWEPTLFDKTDSRPVDHPDWKY
                     ALALMNHTVLETNGKLIPYGQDIRNSNGRALLDRALLGNYVNRCGFPKALVWLMKDSI
                     LPPVLKLENKYLAIAMGAALMTQRNRAENVTEEELNKLVFEPFANPFRVYELYRDVEA
                     FLKVADSGADFYCFNSRGYWKESDDILEPIPLNTSLTLQTAILMDQLKWEHWN"
BASE COUNT          701 a          409 c          468 g          698 t
ORIGIN      
        1 ctttggaaca tttactaaaa gatattggaa gcaagaattc tttaattgta gtggacggca
       61 tattttccat ggaaggggat attgcaaatc ttcctgctat ctgtgattta gcagaaaaat
      121 acaattctga ggttatggtt gacgaagccc attctttagg tgtcttagga aatttgggtg
      181 caggaacaac tgctcatttt ggcttaactg ataagactgc tttcataatg ggaactttca
      241 gtaaatcttt agcatcagta ggtggtttta tagctgccga tgaagctctt atccactatt
      301 tgaaacataa atctagagct cttatttttt ctgcatctct tccacctgca tcaactgcca
      361 gcgttttagc agcattaaaa attatggaag aagaacctga acgcatagcc aagctatggg
      421 aaaacacaaa ctatatgtta tcagaattta aagcaatggg gtataataca ggagatagct
      481 gcacaccagt agttccttta catgtcggca atatgatgac tgcctttaat atgtggcgtc
      541 gtttaggtga agaaggtgtt tttattaatc ccgtagttcc tcctgccgtt ccacccagtt
      601 cctgtttaat tagaacatct tttatggcaa ctcataccca agatcaactc gatatggctc
      661 tggataaatt cagaatgatt ggtaaagagc ttaatataat ttaatatata aatgagaatc
      721 agcgtatatt tttaaaataa aataagccat atatggagga tcaaaatggc aagcaagagt
      781 agtgtggaat actattccaa tattaaagaa atgtctccta tcagagcgat tgccgaaact
      841 ctgatgaata accatctagt tcgcaaagta gatattcgcg aggcatatga attggcaata
      901 aaacaacccg gtgtaagtgt tactgattta ccgatgtacc cggaattcgt aaaactgcat
      961 aatcttccga aagatgccaa aattattaac gattgtcacg gcaatatcat tggtagaacc
     1021 gcaaaagcac gcaggtttta caatcgggta aatccttcac ttaaaaataa gttggagagc
     1081 gattttcggg aagccgtttg gcaaatgcag cattatccgc tcatcaaagc tgaagctatt
     1141 ttgggtatgg ataaggacct aatgctgaaa gccactttta taaccactga aagcgatgct
     1201 gccaatcttt atttttggtt gttgaatttt gcaccttatg aatctctggc gcaagaatat
     1261 gaagcaagtc ctaaacttcc aattcaggat attattttag tcgcttttaa tgaatggact
     1321 tgcaacgatt cttattataa caatgttggc gcaccgcagc tggctttagt agatgaaaaa
     1381 cacaatgtaa tggtcaacct tgggatgcgt tatttcgggg aacgcaaaaa aggaacttta
     1441 actcttgcct ggacttctgg tatccgaatt gggatggctg cctgccatgg tggtattaaa
     1501 gaaattgatt ttggtacttg tgaaaatcct gaatatcata aattgggtaa gcgttctata
     1561 gctttttatg gactttctgg aacaggaaaa tccagccata caaataatgc cgataatgct
     1621 ggaactttac ctaagggctt ttccaaggtt gttttgcatg atgatgcttt ccagattgat
     1681 ctcgaaaata agcagtgccg tgcatgggaa ccaactttat ttgataagac agatagccgg
     1741 ccagtggatc atccagattg gaaatatgcc ttagctttaa tgaatcatac cgtgttagag
     1801 acaaatggca aacttatacc ctacggacag gatattcgta attccaatgg tagagcgtta
     1861 ttagaccgtg ctcttttggg aaattatgta aaccgttgcg gtttccccaa ggccttggtt
     1921 tggctgatga aagattctat tttacctcca gttttaaaat tggagaataa atatttggca
     1981 atcgctatgg gagcagcatt aatgactcag cgcaaccgtg ctgaaaatgt taccgaagaa
     2041 gaattgaata aactcgtttt cgaacctttt gccaatcctt ttagggttta tgaactctat
     2101 cgggatgtgg aagcattttt gaaagtagct gatagcggag cagatttcta ctgcttcaat
     2161 tcccgtggct attggaaaga atctgatgat atccttgaac ccattcctct aaatacttct
     2221 ctcactcttc aaactgccat tttgatggat caattaaaat gggaacattg gaatat
//
LOCUS       DAORHB010000185         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_58823_length_2267_cov_5.107595, whole genome shotgun sequence.
ACCESSION   DAORHB010000185 DAORHB010000000
VERSION     DAORHB010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58823_length_2267_cov_5.107595"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..674
                     /locus_tag="PKM80_05405"
     CDS             75..674
                     /locus_tag="PKM80_05405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNV93382.1"
                     /translation="MNLKFQYNKISQLQLIKQLAVRQRALPTLKNKESALRLEVKKAR
                     DNALALDQKVKERTAELDAFMKLFVEFDPDIVKIKNVEIKTRKIAGVKTPLLENIEFE
                     IADYNLFKAPSWFPEGISLLKEICQLQIERDFFIRKMHILEQVRKKTTQKVNLYEKVQ
                     IPAFEDAILKIKRYLEDEENLSKASQKILKSRFESETRK"
     gene            671..>2267
                     /locus_tag="PKM80_05410"
     CDS             671..>2267
                     /locus_tag="PKM80_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNV93383.1"
                     /translation="MIEKMRKYTFILYHQEYESFLVELQKLGLVHIIRSRDDKTESQT
                     KNLELMQEYGECIKFLTKLKSIAPKQTNPLPTKALLNRINEAREEKEKLQHSIDMVKK
                     QIRDLTPWGHFDYNLVHKLKEAGLTIRFHTCLKNHFKPEWQENYPVKIINEVGGIVYF
                     IVITDDGDPCLEADTFSFHKHTLQELEDHLSELQNKQRDIDEYLYSIAPTAIEMFNEE
                     LKRLSNEYEFEDAMQQGEFEAENTIRLISGWIPVSLEQGLKDFLEKNNVIYFAEEAKA
                     EENPPIKLKNNWFARLFEPISKMYMLPYYNEFDLTPFFAPFFALFFGFCNADIGYGIV
                     IIILAVILKLKLKNKGMKDIMSLLIIFGASSIIMGWVMGSALAYDLKTIPALADKILI
                     KDNNQIFNYALLLGAIQILFGIAISAVKTIRNNGIKFGLSTIGTFLLLLGLTVLGSSL
                     LGTDISKVQPYLKYPIYIGLAMILLFNSPGKNIILNILNGLWLLYQIITGYFGDILSY
                     IRLFALSVSSAILGFVMNSIGSQM"
BASE COUNT          768 a          378 c          430 g          691 t
ORIGIN      
        1 cttttccaga agtatttcaa ccgtgctgaa ataggtatca aagacgaatt tatgagttta
       61 tattggaaga aggaatgaat ctgaagtttc agtataataa aatatcccaa ttgcagttaa
      121 ttaaacaact ggcagttagg caaagagcat taccaactct taagaacaag gaaagtgcat
      181 tacggttgga agttaaaaaa gcacgggata atgcattggc tttagatcaa aaagtgaaag
      241 aacgcactgc ggaactcgat gcttttatga aactatttgt ggaatttgat ccagacatag
      301 tgaagataaa aaatgtggaa atcaaaaccc gcaaaattgc tggcgtaaaa actcctctat
      361 tggagaacat agaatttgag attgcggatt ataacctttt taaagcacct tcctggtttc
      421 ctgaaggaat ttccttacta aaagaaatct gccagctgca aattgaaagg gactttttta
      481 tccgcaaaat gcatattttg gagcaggtgc gtaaaaagac cactcaaaaa gtgaatcttt
      541 atgaaaaagt gcaaattcct gccttcgaag atgctattct aaagataaaa cgctatttgg
      601 aagatgaaga gaatctatcc aaggcgtctc aaaaaatact aaaatcccgc ttcgaatcgg
      661 agactcgaaa atgattgaaa agatgcgcaa atacaccttt attttgtatc atcaggaata
      721 cgaaagcttt ctggttgaac tgcaaaaatt gggtttggtg catattattc gcagtcgtga
      781 tgacaaaaca gaatcccaaa ccaaaaatct tgaattgatg caggaatatg gtgaatgtat
      841 aaaattctta accaagctga aatctattgc ccctaaacag acaaatcctc tacctacaaa
      901 agcattactg aataggatta atgaagccag agaagaaaaa gaaaaactgc aacactctat
      961 cgatatggta aaaaagcaaa tccgggattt aaccccttgg ggtcatttcg attacaatct
     1021 ggttcacaaa ttaaaagaag caggtttgac gatccgtttt catacctgtt taaagaatca
     1081 cttcaaaccg gaatggcagg aaaattatcc tgtaaagatt atcaacgaag tgggtgggat
     1141 tgtttatttt atagtgatta ctgatgatgg cgatccgtgt ttggaagcag atactttttc
     1201 tttccataaa cataccttgc aagagcttga agatcatcta tcagaactac aaaataaaca
     1261 gagagatata gatgaatatt tatattccat agcgcctaca gctattgaaa tgttcaatga
     1321 agaattaaaa cgcttaagca atgaatatga atttgaagat gctatgcaac aaggtgaatt
     1381 tgaagcagaa aatacgatcc ggcttataag cggttggata cctgtaagtt tagaacaggg
     1441 tttaaaggac tttctggaaa agaacaatgt aatatatttt gctgaagaag ccaaagcaga
     1501 ggaaaatcct ccgatcaaac taaagaataa ctggtttgcc cggctttttg aacctatcag
     1561 taaaatgtat atgctgccct attataatga gtttgattta actcctttct ttgctccctt
     1621 ttttgcgctt ttctttggct tttgcaatgc ggatattggc tatgggatag taattattat
     1681 tttggctgta atcttaaaac taaagttgaa aaacaaaggt atgaaggaca ttatgtcctt
     1741 gctaattatc tttggcgctt cttccataat aatgggatgg gtgatgggaa gtgctttggc
     1801 ttatgatcta aaaacgattc ctgctttagc agataaaatc ctgataaaag ataataatca
     1861 aatcttcaat tatgctctgc ttttaggagc tatacaaatt cttttcggaa ttgccatctc
     1921 agctgtaaaa acaatacgga acaacggaat caaatttggt ctctctacta ttggtacctt
     1981 cttgttatta ttgggtttaa ctgttcttgg gtcttcactt ttaggaaccg atatcagtaa
     2041 agttcaacct tacctgaaat atcctattta tataggattg gcaatgattc tactgttcaa
     2101 cagtcccggt aaaaacatta ttctcaatat tctcaacgga ttatggttgc tctatcaaat
     2161 tataaccggt tattttggag atattctttc ttacatccgt ctttttgctt taagcgtttc
     2221 cagcgctatc ttgggctttg tgatgaattc cattggcagt cagatgg
//
LOCUS       DAORHB010000186         2198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_61763_length_2198_cov_4.101260, whole genome shotgun sequence.
ACCESSION   DAORHB010000186 DAORHB010000000
VERSION     DAORHB010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61763_length_2198_cov_4.101260"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="ftsA"
                     /locus_tag="PKM80_05415"
     CDS             complement(<1..859)
                     /gene="ftsA"
                     /locus_tag="PKM80_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNV93384.1"
                     /translation="MKQSIITALDIGSSNVRCIIAKEIGEGRLEILGIGESPSEGIEC
                     GIVKDIQALSGCVAKAISTAENDAKIAATNIYCNITGDHIRTQIGDGRISIPSEIPNE
                     PGEITQEHTEQVINDAKNSVKIQKGLERCRILHGIPHHFIIDNQDDIHNPVNMNGFHL
                     IARVYTILAELTPLRNLNKCIQLAGYDIDPENFVLNHIAVAESILSEDERRLGALVLD
                     IGGGTCDLSIFNRGTLEKILVIPMAGKNITEDLAIGLKTTLGNAEYIKIEYGNALANS
                     VDQTEEIDVE"
     gene            complement(856..1623)
                     /locus_tag="PKM80_05420"
     CDS             complement(856..1623)
                     /locus_tag="PKM80_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNV93385.1"
                     /translation="MELNKGTRKRRGNSRYYLFFFFALIGVAIIGSGIWFGLTNIDLF
                     KFQKMQISGNKVIPDTLFYKITSPYIGMNLFTVPSSEIKAKLNTLSRVKNVKLHKRLP
                     DTIKLDITERKAAIYLKTIEGDLYPVDSEGVVLIIYTPVYKEDIPIYSTYLSNNQIKP
                     GIKLKYSGLQKVLQLHQRVVKEAPDFLPQISEYYLIDNTINIIDAKTGTRIIPSEEDF
                     ATQISRYRFVQENGNINRNSVVDLRFKNQVVVKAGNK"
     gene            complement(1723..>2198)
                     /locus_tag="PKM80_05425"
     CDS             complement(1723..>2198)
                     /locus_tag="PKM80_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNV93386.1"
                     /translation="GLQEFNGVYRRFDMKGEAAGITVYDDYAHHPTEIKATLEGFKDS
                     VKRRIVVLFQPHLYSRTRDFYEEFGKSFFSCDCLILAPIYPAREQPIPDITSKLIADA
                     AIQSGHHQVHLVESNSEIVSRTLPLLKEGDILITMGAGNIWKYGEEILGKLKELC"
BASE COUNT          665 a          416 c          357 g          760 t
ORIGIN      
        1 cttcaacatc aatttcttct gtttggtcca cactattggc taaggcattt ccatattcaa
       61 tcttgatata ttcggcatta cctaaagtgg ttttaagacc aatagccagg tcttcagtaa
      121 tgttttttcc tgccataggg ataactaata ttttttccaa ggtgccgcga ttaaaaatgg
      181 acaaatcaca tgttccgcct ccaatatcaa gaactaaagc tcccaggcga cgctcatctt
      241 cacttaaaat tgattcagct acggcaatat gatttaaaac aaaattttcc ggatctatat
      301 cataacctgc cagttgaata cacttattta gatttcgcaa tggagtaagt tcagcaagaa
      361 tagtatatac tctagcaatt agatggaaac cattcatatt tacaggatta tgaatatcat
      421 cctgattatc gattataaag tgatgcggaa taccatgcaa aatacgacaa cgttccaaac
      481 ctttttgaat tttaacacta ttcttagcat cgtttataac ctgttctgta tgttcctgtg
      541 taatttcacc aggttcatta ggaatttcgg aggggataga aatgcgtcca tcaccgattt
      601 gagttctaat atgatcaccg gtaatattgc aatagatatt ggtagcagct attttagcat
      661 cattctcagc tgtggagatt gcttttgcta cacaaccgga aagagcttga atatctttta
      721 caatgccaca ttcgatacct tcggatgggc tttctccgat gcctaaaatc tctaagcgtc
      781 cttctccaat ttctttagca ataatgcaac gaacattgga agaaccaata tctaaagctg
      841 tgatgatgga ttgtttcatt tattacctgc cttaacaaca acttgatttt tgaagcgcaa
      901 gtcaactacg ctgtttctat ttatatttcc gttttcctga acgaatcgat aacgggaaat
      961 ttgcgttgca aaatcttcct ctgatggaat tatccgagtt ccagttttag catcgattat
     1021 gttaatagtg ttgtctatta aataatattc tgaaatctga ggcaaaaagt ctggtgcttc
     1081 tttcactact ctctgatgaa gctgtaaaac cttttgtaat ccagagtatt ttaattttat
     1141 tcccggtttg atctgattgt tacttaagta agtactataa attggtatat cttctttata
     1201 aactggcgta taaatgatta aaactacacc ttcggaatct acaggatata gatctccttc
     1261 tattgtttta agataaattg ctgccttgcg ttcagtaata tcaagtttaa tagtatcggg
     1321 taaacgctta tgtaatttaa cattcttaac cctcgataga gtatttaact ttgcctttat
     1381 ctcagaagaa ggaactgtaa ataaattcat tcctatatag ggactggtaa ttttataaaa
     1441 taaagtatcg ggaataactt tattgccgct tatttgcatc ttttggaact taaaaaggtc
     1501 tatgttagta agcccaaacc agatacccga tccaataatt gctacaccta ttagtgcaaa
     1561 gaaaaagaac agataatatc tgctattacc gcgtcgctta cgagtacctt tgtttagttc
     1621 cataacttta tgcctcaaag tcctttatct gtttaactga gattttagta aaatcaatct
     1681 cagctttaca agttactact tttttatatg ttatgctttg tgtcaacata attcttttaa
     1741 ctttcctaaa atttcttctc catacttcca aatgttacct gctcccattg tgattaggat
     1801 atctccctct ttaagcaggg gaagagttcg gcttactatt tccgaattag attcaactaa
     1861 atgaacttga tgatgaccac tttgaatggc tgcatcagca attaatttgg atgttatatc
     1921 tggaataggt tgttcccggg caggataaat tggggccaaa atgaggcaat cacaagaaaa
     1981 gaatgattta ccgaattctt cgtaaaaatc tcttgtccga gaatataaat gaggttgaaa
     2041 tagtactaca atccttcttt taacactatc tttaaaacct tccagagtag cttttatttc
     2101 tgttggatgg tgagcataat cgtcataaac tgttattcct gcggcttctc ctttcatatc
     2161 aaaccgtcta taaacaccgt tgaattcttg caggccta
//
LOCUS       DAORHB010000187         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_63136_length_2167_cov_4.395833, whole genome shotgun sequence.
ACCESSION   DAORHB010000187 DAORHB010000000
VERSION     DAORHB010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63136_length_2167_cov_4.395833"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1293
                     /gene="nusA"
                     /locus_tag="PKM80_05430"
     CDS             109..1293
                     /gene="nusA"
                     /locus_tag="PKM80_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNV93387.1"
                     /translation="MDKEMIQNIILESVISTLQKRLEPESELEVYIDDLSGNIKVKFQ
                     SLVVEREEGLGQISLTEAREEYDPEVQLGQFIEKTMSLYEFEPKLIKTIQKIIQDKIR
                     KLEDDKIQNDFNKQKHTIVTGKIKAIDDFGGYIIDIGYTDALLPLDEQIENEFYRVGE
                     NIKAYVVNIRSGKDGVVIVLSRTNPEFVKKLFEAEIPEIFTGEIKIRKIVREPGIRTK
                     VELETANPKIDPVIACVGPKGTRIDSIRKELHGEQIDIVIHSDDPEKMVENALGVTGI
                     KRVIIERNHSASVIVEESDKVMAIGKQGKNVKLAAKLVGLKIDIYTMPEYEEKMAKER
                     RTISHIAELDGVTPKIAEVLRQAGYTSVQDVYMASIEELRNLEGMGQKTAERLKEAAK
                     YF"
     gene            1293..1532
                     /locus_tag="PKM80_05435"
     CDS             1293..1532
                     /locus_tag="PKM80_05435"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HNV93388.1"
                     /translation="MSNKNSKAKHIPIRTCVVCKKKVDQNRLLNFFHTTSGIVFDCNR
                     IIQVRKYYLCPNEECFKGLSKWQKRYQKKSLGSIR"
     gene            1529..1858
                     /locus_tag="PKM80_05440"
     CDS             1529..1858
                     /locus_tag="PKM80_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNV93389.1"
                     /translation="MNSKILTLMQFARKAGKLVVGTDACERAIGRKEVFLLILAQDNS
                     ERTKNKMKHIAGAAKKSLEVIETGTQEEISSALGLSITGVFGIKEKHIAGKIMEYWLS
                     ETERRIS"
     gene            1855..>2167
                     /locus_tag="PKM80_05445"
     CDS             1855..>2167
                     /locus_tag="PKM80_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HNV93390.1"
                     /translation="MTIRVYDLAKELKISTMALKKHLTDLGVNVKSHMSLIDEEVADK
                     IRIKYKEQVDAEKRAERDRKRLIEMRQAAKAKQETPEPEAKKEPEIIAPVVEQITIVP
                     EP"
BASE COUNT          790 a          324 c          498 g          555 t
ORIGIN      
        1 gtatgtggcg gtggatgaag ccgatgcgcc ggtggggctg atgaaaattc tgccgtcgaa
       61 aagaaagccg agaaaatcac ggcggtttat gacgaactga taaaacagct ggacaaggaa
      121 atgattcaga atattatatt ggaatctgtt atatcaacat tgcaaaagcg attggaacct
      181 gaatctgagc tggaagttta catagatgat cttagtggta atatcaaggt aaaattccaa
      241 agtttagtag tggaacgaga agaaggtctt ggccagattt ctctaaccga agcacgcgaa
      301 gaatatgatc ctgaagttca actcgggcaa ttcatagaaa aaacgatgtc tctttatgag
      361 tttgagccca agctgatcaa aactattcag aagataattc aggataagat ccgtaagctt
      421 gaagatgaca aaattcagaa tgattttaat aaacagaagc atacaattgt aactggaaag
      481 atcaaagcca tcgatgattt tggcggctac attattgata taggttacac agatgcgtta
      541 ctacctttgg atgaacagat tgaaaatgag ttttaccgtg tgggagaaaa catcaaagcc
      601 tatgtagtta atattcgttc tggaaaagat ggagttgtta tagttctttc ccgcaccaat
      661 cctgaatttg ttaaaaagtt gtttgaagcg gagatacctg aaatcttcac cggcgaaatc
      721 aaaatccgta aaatcgttcg tgagcctgga attcgaacca aagttgaatt ggaaactgct
      781 aatccaaaaa tcgatcctgt gattgcttgt gttggaccaa aaggaactcg cattgattcg
      841 atccgtaagg aattgcatgg tgagcaaata gatattgtta ttcatagtga cgatccagaa
      901 aagatggttg aaaatgcttt aggtgtaaca ggtataaaaa gggtaattat agagcgtaat
      961 cattctgcca gtgtaatagt tgaagaatcg gacaaagtga tggcaatagg taaacagggg
     1021 aaaaatgtaa aacttgctgc caaactggta ggtttgaaaa tagatatata tacgatgcct
     1081 gaatatgaag aaaaaatggc aaaagaacgt agaactataa gtcatatagc agaacttgat
     1141 ggagttactc caaagatagc tgaggtttta agacaagccg gttataccag tgttcaagat
     1201 gtttatatgg cttctataga agagcttcga aaccttgaag gaatggggca aaaaacagca
     1261 gaacgcctaa aagaggcagc aaaatatttc tgatgtcgaa taaaaatagt aaagctaaac
     1321 acattcctat ccgcacttgt gtagtgtgca agaaaaaggt tgaccagaat cgactgctaa
     1381 atttttttca cacaacaagt ggaattgttt ttgattgcaa caggataatt caggtgcgca
     1441 agtattattt gtgcccaaat gaagagtgtt ttaaaggtct ttcaaagtgg caaaaacggt
     1501 atcagaaaaa aagtttagga agtataagat gaattctaag atcctaactt tgatgcagtt
     1561 tgcccgaaaa gcaggaaaat tggttgttgg taccgatgct tgtgaaagag ctattggcag
     1621 aaaagaagta tttctgttga tcctggctca ggataattca gagcgaacta aaaataagat
     1681 gaaacatatt gctggtgcag cgaaaaaatc ccttgaggtt attgaaacag gaacccagga
     1741 agaaataagt tctgctttgg gattgtctat aaccggtgta tttggtatta aggaaaaaca
     1801 tatagccggc aaaattatgg agtattggct ctctgaaaca gagaggagga taagttgaca
     1861 ataagagttt atgatttagc taaagagctg aaaataagca cgatggcact aaaaaagcac
     1921 ctcacggact tgggtgtgaa tgttaaaagc catatgagct taatagatga agaagttgca
     1981 gataaaatac gcattaaata taaggaacag gttgatgctg aaaaaagagc tgaaagagat
     2041 cgcaaacgat taatagaaat gcggcaagca gctaaagcaa agcaagaaac cccagaacca
     2101 gaagcaaaaa aagaacctga aattattgct cccgtggttg agcaaattac aattgttcct
     2161 gaaccag
//
LOCUS       DAORHB010000188         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_63187_length_2166_cov_3.498342, whole genome shotgun sequence.
ACCESSION   DAORHB010000188 DAORHB010000000
VERSION     DAORHB010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63187_length_2166_cov_3.498342"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKM80_05450"
     CDS             <1..538
                     /locus_tag="PKM80_05450"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNV93391.1"
                     /translation="KVEITEINALSPLGISFRVENPSFIGADLIANAYAALHKYKSSS
                     IIIDLGTATTIELISNERRFEGAIIAPGMKLGAEILFQRAAQLYEIELVQPPVLLGLN
                     TTDAMLSGVVYGHSIMLDALVVKIKEQYSQLAPIRTILTGGMASLVKPSMKEIDVVDR
                     HLTLDGFYLAFCRILQQK"
     gene            545..>2166
                     /locus_tag="PKM80_05455"
     CDS             545..>2166
                     /locus_tag="PKM80_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93392.1"
                     /translation="MLKKLFLCLLLIAFTLLWANEPTGEVNTNPEQEETGQKGLFPVK
                     PFRQKKEGQHQLDPLTEKAMYPRLYIPDYNPVINNRIEVYKINKAMNYYKARPLEIPA
                     FAPEYKEKIDFTNNKVILSVQVGDYKIAPDVIISFDRYFSNMQMKAFHKSIIANIKTQ
                     AQATQTVSSGLFKDLTLLPEIAMPKAMQKVLGSSAGRLNLDGTQKLTLQASNTRRKQV
                     PIYDESGKNVFDLKMEMETNLRLSGTIGDKIAVNFKYNSKQDEQLFDMNNVNVKYTGY
                     DDEFVQSIEGGNIALSLGGSRYVSYSASSQGLFGITSKFKYGNLDLNLIASKEESQKS
                     TQTYVGKSQADSSTVSSWKYAKRTMYYLHDPYQLYQLKTGSNIPPGWVNNAIDTAPDG
                     SWLVNENYLPVSGTVRLFYDDANYTNNTAAAKGDTIYYSNDVRPPYEPWYDELIEGTD
                     FVTDYDSGIITVLKNIDRTATLGVQYTRRDGVQVPPPNPDTSILHVKVLRKKYQEYIP
                     YNSNDELNTWHLQMRNIYDMGRTNIKNDGFEL"
BASE COUNT          758 a          374 c          396 g          638 t
ORIGIN      
        1 caaggttgaa ataactgaga ttaatgcgct cagtccttta ggtattagtt tcagagtaga
       61 aaatccgtct tttattggag cagatttaat agctaatgcc tatgctgcct tgcacaaata
      121 taaaagttcc tcaataataa tagatttagg aactgctact acaattgaac tgatcagcaa
      181 cgaaagaaga tttgaaggag ccataatagc tcctgggatg aaacttggtg ctgaaatatt
      241 atttcaaaga gctgcccaat tgtatgaaat agaacttgtt caaccgcctg ttcttttagg
      301 tttaaacact acagatgcaa tgctttctgg agtagtttat ggtcattcta taatgttaga
      361 tgcccttgtt gttaaaatca aagaacagta ttcacagcta gcaccaattc gaacaatctt
      421 gactggtggt atggcttctc tggtgaaacc ctctatgaag gaaattgatg ttgtagatag
      481 acacctcact ttggatgggt tttatttggc tttttgcagg attttacagc aaaaatagta
      541 ctttatgctg aaaaagctat tcctttgtct cttacttata gcgttcacct tactgtgggc
      601 taatgaacct actggagaag ttaatacaaa ccctgagcaa gaagaaacag gacagaaggg
      661 acttttcccg gtgaaacctt tcagacaaaa aaaggaagga caacatcaac ttgatccact
      721 aacagaaaaa gcaatgtatc ctcgtttgta tattcccgat tataatcctg taataaataa
      781 tcgtattgaa gtttataaaa tcaataaagc aatgaattac tataaagcaa gaccactgga
      841 aataccagct tttgcaccag aatataaaga aaagatagat tttactaata ataaagtaat
      901 cctcagtgtg caggttggcg actataaaat tgctccagat gttattatta gctttgatcg
      961 ctattttagt aatatgcaaa tgaaagcctt tcacaaatct attattgcca atataaaaac
     1021 acaagctcaa gcaacccaaa cagttagcag tggtctattt aaagatctta ccttgcttcc
     1081 agaaatagct atgcccaaag ctatgcaaaa agttttaggt tcttctgctg gacgattaaa
     1141 tttagatggt acccagaaac ttactctaca ggcaagtaac acacgcagaa aacaggttcc
     1201 tatttatgat gaatcaggaa aaaatgtttt cgacctaaaa atggagatgg aaactaacct
     1261 aaggctgtcc ggaacaatcg gtgacaaaat agcagttaat ttcaaataca attccaaaca
     1321 ggatgaacag ctcttcgata tgaataatgt aaatgtaaaa tataccggtt atgatgatga
     1381 atttgttcaa agtatcgaag gcggaaacat agctctttct ttaggtggat cgcgctatgt
     1441 tagttattct gcaagttcac agggactttt tggtatcacc agtaaattca aatatggcaa
     1501 tttggattta aatcttattg ccagtaaaga agagagccaa aaaagcactc aaacctatgt
     1561 aggtaaaagc caagcagatt cttctacagt aagcagctgg aaatatgcta aacgaacaat
     1621 gtactatctg catgatccct atcagttata tcaattaaaa acaggttcta atatacctcc
     1681 aggctgggtt aataatgcaa ttgatactgc tcctgatggc tcatggcttg tgaatgaaaa
     1741 ctatttaccc gtaagtggaa ccgtgcgttt gttttatgac gatgctaatt atacaaataa
     1801 cactgcagca gcaaaaggag ataccatcta ttattctaat gatgttagac ctccttatga
     1861 accctggtat gatgaattaa ttgaaggaac agattttgtt acggattacg atagcggaat
     1921 tattactgtt ctaaaaaata ttgatagaac cgctacttta ggagtgcaat acactcgtcg
     1981 agatggagtt caagttcctc ctccaaatcc tgatacaagt attttacatg taaaagtatt
     2041 aaggaaaaaa taccaagagt atattcctta taactccaat gatgaattaa atacctggca
     2101 tttacaaatg cgcaatatct atgatatggg cagaacaaat ataaagaatg atggatttga
     2161 actgca
//
LOCUS       DAORHB010000189         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_63626_length_2156_cov_4.402665, whole genome shotgun sequence.
ACCESSION   DAORHB010000189 DAORHB010000000
VERSION     DAORHB010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63626_length_2156_cov_4.402665"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..837)
                     /locus_tag="PKM80_05460"
     CDS             complement(217..837)
                     /locus_tag="PKM80_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV93393.1"
                     /translation="MENLGKYLLELRLQRGYSYQDIWNELRFKEDQIKAIEENRLSAL
                     GPYGIAKAVVFNYARYLEADLNSVMNAFSIMMPESNKPEFKPATTVKEKKIMLSTNFM
                     WTLGILVIIIILGSILWYAYNQNWLRPPNFFTAKKSDSTAVSIKEQKEETKPDTLRQH
                     LQAISEAIPKTNNIKIDNLDSKTVPPDTTDYLGNILGDSPINVPIH"
     gene            complement(825..1613)
                     /gene="dapF"
                     /locus_tag="PKM80_05465"
     CDS             complement(825..1613)
                     /gene="dapF"
                     /locus_tag="PKM80_05465"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNV93394.1"
                     /translation="MLIPFIKMNAQGNDFVILDYFAPELVTLKELNYSSLALEVCRPH
                     FGIGADGLVLLKPSTEFDAQMIIFNADGSQAEMCGSALRCVTWLLYKKTEKREFEILT
                     DSGVKKCQVNNDKTITANLGLPKMLKRKYNAEGFTGDLIEIGNPHFIVWQNNSDDDPH
                     IKYGPLLEHHQGFPDTVNVHFAYLINRNEIAIKIWEKGCGATFACGTGASATVYSGII
                     NGFLDNEVKVNMPGGTVKISATAQGYLLSGEVYETFQGVFKWRI"
     gene            complement(1607..>2156)
                     /gene="dapB"
                     /locus_tag="PKM80_05470"
     CDS             complement(1607..>2156)
                     /gene="dapB"
                     /locus_tag="PKM80_05470"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNV93395.1"
                     /translation="QKVLSYKKNMVVGTTGWFDHLEEVQKMVENSASGLIYGANFSIG
                     MNIFNRLVAEATKAFDRFPDYDVYGLEMHHNQKADSPSGTAIELSKTILANSSHKKTT
                     LFDPAYRKIEPSELHFVSLRAGSIPGTHKVGFDSEADTIELSHCVRSRSALASGALQA
                     AKWIKDRKGFYSFSDMVSSVLC"
BASE COUNT          686 a          410 c          370 g          690 t
ORIGIN      
        1 aatttttatt gttcactaat taatataatc gaagccacaa aaaaagcaag aaaaatttta
       61 gcaatctgac cattagattg ctaaaatttt ttgccgaatt agctaactga ctgaatataa
      121 agatgttaca atagcaaaaa aattataatt tttcactatc tcagatttgg attttttaaa
      181 aagttcttag agggtagctg taagataata atctggctaa tggataggga cattaatagg
      241 gctatcaccc aaaatattgc caagataatc agttgtatct ggagggacag ttttgctatc
      301 taaattatca atttttatat tattagtttt aggtatggct tcactaattg cctgtagatg
      361 ttgacgcaaa gtatcaggtt ttgtttcttc tttttgctct ttgatgctaa cagctgtaga
      421 atcggatttt ttggcggtga agaaattcgg gggtctaagc caattttgat tataggcata
      481 ccagagaatt gaacccagga taataataat aactaaaatc cccagggtcc acataaaatt
      541 ggtggagagc ataatctttt tttctttaac tgtggtagcg ggtttaaatt caggtttatt
      601 actttccggc atcataatgg aaaaggcatt catcacagaa ttcaaatcgg cttcaaggta
      661 acgagcataa ttaaaaacaa cagcttttgc aatgccatag ggtcctaatg cagataagcg
      721 attttcttca attgctttta tctgatcttc tttgaaacga agttcattcc agatatcctg
      781 atagctgtaa ccacgctgta aacgcaattc cagaaggtac ttacctaaat tctccatttg
      841 aatactcctt ggaaggtttc ataaacttcc ccacttagta aatacccctg tgcggtggct
      901 gaaattttta cagttcctcc tggcatatta accttaactt cgttatccaa aaagccatta
      961 atgataccac tatagacagt tgctgaagct cctgttccac aggcaaaagt tgctccacaa
     1021 cctttttccc aaattttgat agctatctca tttctattta tcaggtaagc aaaatgaaca
     1081 ttcacagtat cgggaaaacc ctggtgatgt tccaaaagtg gaccatattt tatatgtggg
     1141 tcatcatcag aattattctg ccaaacaata aaatggggat tgccaatttc aatcagatct
     1201 cctgtgaagc cctcagcatt gtactttctt tttagcattt tgggtaaacc caaattagca
     1261 gttattgttt tatcgttgtt tacctggcat tttttaactc cagaatcagt aagaatctca
     1321 aattcccttt tttctgtttt cttgtataga agccaggtta cacatcgtaa agcagagcca
     1381 cacatttcag cttgtgaacc atcggcattg aaaattatca tttgagcatc aaattctgtg
     1441 gagggcttta ataaaactaa gccatcagcc ccaatgccaa aatgaggtct acatacttct
     1501 aaagccaggg aggaataatt aagttccttt aaagtaacaa gttcaggcgc aaaataatcc
     1561 agaataacaa aatcgttacc ctgtgcattc attttaataa aaggtatcag cataaaacgg
     1621 aagaaaccat atcgctaaag ctataaaagc ccttgcggtc tttaatccat tttgctgcct
     1681 gtaaagcacc agaagctaat gcactacgag aacgcacaca atgactaagc tcaattgtgt
     1741 cggcttcact atcaaaaccg actttatgtg ttcctggaat tgaaccggct cgtaatgaaa
     1801 caaaatgtaa ttcactcggt tcaatttttc tatatgcagg atcaaataga gtagtttttt
     1861 tatggctgct gttggctaat atagttttgg aaagctcaat agctgttccg gaaggactat
     1921 ctgctttttg attatgatgc atttccaaac cgtagacatc ataatcggga aaacgatcaa
     1981 aggctttcgt tgcttctgct actaagcgat taaagatatt cataccaatg gagaaattag
     2041 ctccataaat cagacctgaa gccgaatttt ccaccatttt ttgtacttcc tctaaatgat
     2101 caaaccagcc ggtagttccc acaaccatat ttttcttata ggaaagcact ttttga
//
LOCUS       DAORHB010000190         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_64773_length_2134_cov_3.692160, whole genome shotgun sequence.
ACCESSION   DAORHB010000190 DAORHB010000000
VERSION     DAORHB010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64773_length_2134_cov_3.692160"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PKM80_05475"
     CDS             complement(<1..1239)
                     /locus_tag="PKM80_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNV93396.1"
                     /translation="MIFRSNRQKDDQLTGWLKIWCMLIFLFLSGFLLAQTRTVKVGIS
                     QNDPKMFIEPSGKPAGIFIDILEAIAAKEDWKLEYYEGSFDAIYYRVAKGDLDLMPDV
                     AISPTREKDLDFHKEPVLSSWSQIYTRKGLEINSILDLDGHSIAVLEKSIQQNVIESW
                     IKGFGLKVKIIKVHNFDAAFAAVANGIADCAVSNYLYGRRHCKQYGLVDTGVVFEPAT
                     LYFATTKGKNQDLLDAIDKHLLTMKKVPNSAYYAALKRWVSKDEGFMLPAWLKFTILI
                     VILALVLTVIYGLTLKHQVNIRTLELRKSNQEMEQRIIERTAELEKAMLKAKEADMLK
                     SAFLATMSHELRTPLNSIIGFTGILLQELPGHINEEQRKQLTIVQNSAHHLLDLINDI
                     LDISKIESGQLDLHYESFFLN"
     gene            1577..>2134
                     /locus_tag="PKM80_05480"
     CDS             1577..>2134
                     /locus_tag="PKM80_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93397.1"
                     /translation="MNKVFYRPLIIVVIAVILVELIARVIPDSDNKIFPIKKLSWLES
                     IEPKDTIEPTDTIVVKEATAIPNNLLPLQPFIQKMNNSAKSIRIAYYGDSIIEGDLIT
                     GKLRQELQSVHSGSGVGMIGITSIVSGFRQTIKHKFSRNWESISFMSTGRNNIPLGIT
                     GYTYIPRNYYIAEKVIEPLQLDSLAV"
BASE COUNT          741 a          373 c          348 g          672 t
ORIGIN      
        1 gttcaagaaa aatgattcat aatgcaaatc cagctggcca gattcaattt tggagatgtc
       61 taaaatatca ttaatcaaat ccaaaagatg atgagcactg ttttgcacta tagtaagctg
      121 ttttctttgc tcttcattta tatggccagg taattcttgc agtaaaatac ctgtaaaacc
      181 gataatagaa tttaagggag tgcgtaattc atgagacatt gtagctagaa acgcagattt
      241 taacatatca gcctctttgg cttttagcat tgctttttcc agctctgcag tacgttcaat
      301 aattctttgt tccatttcct gattactttt tcgcaattcc aaagtgcgaa tatttacttg
      361 atgttttaaa gtaagaccat agataactgt taacactaaa gccaggataa ctatcaatat
      421 agtgaatttc aaccaggcag gtaacataaa accttcatct tttgaaaccc atctttttaa
      481 agcagcataa tatgcagaat taggcacttt cttcatagta agtaagtgtt tatctatggc
      541 atccagaaga tcttggtttt tacctttggt tgttgcaaaa tacaaggttg ctggttcaaa
      601 aacaacacct gtatctacca gaccatactg tttacaatgc cttctgccgt atagataatt
      661 ggaaacagca caatctgcaa taccgttggc aacagcagca aaagctgcat caaaattatg
      721 aacttttatg attttaactt tcagaccaaa acccttaatc catgattcaa ttacattttg
      781 ttgaatggat ttttccagca ccgcaataga atgaccgtca agatcaagta tggaattgat
      841 ttctaaacct ttacgagtat aaatttgaga ccaagaagat agaaccggtt ctttatgaaa
      901 atccaaatct ttttctcggg ttggagaaat tgccacatct ggcattagat ctaaatcccc
      961 tttcgccaca cgataataga ttgcatcaaa gctaccctca taatattcaa gtttccaatc
     1021 ctctttagct gcaattgctt ctaaaatatc aataaagata cccgctggct taccagaagg
     1081 ttcaataaac atcttggggt cattttgaga aatacctact ttaacagttc tggtttgagc
     1141 taataagaac ccagataaaa atagaaagat taacatacac catatcttaa gccaacctgt
     1201 caattgatca tctttctgac ggttgcttct aaaaatcata gtatcctcct caaattacct
     1261 ttttgtaccg atgttactga aaagacattg gattcataag aaaatgacaa aaagtcattc
     1321 tccaaaagag gaatatatcc ttatgtatac atatataata aagtaaaaaa acaaaaagcc
     1381 aaatagttct tgacgattga atgatgtgta aaaattggct ttaataatgt ggaagaatga
     1441 tatcttattg ttccacacac aatagagtta cacggttacc gaaaaattat ccagtaactt
     1501 aagtactgta gctcagagag ttgacgagga aatcaaccaa ccagttaata atacaaagat
     1561 gttttaaaag gtagatatga ataaagtttt ttatcgaccc ctgattatag tagttatagc
     1621 tgtaatatta gtggagttaa tagcaagggt tattccagat tcggataata aaatatttcc
     1681 tattaagaaa ctgtcttggc tggaatctat agaaccgaaa gatacgatag aacctacaga
     1741 tacaatagta gttaaagaag ctactgcaat tcccaacaat ttattaccct tacagccatt
     1801 tatacagaag atgaataact cagctaaaag cataagaatt gcctattatg gcgattctat
     1861 tattgaaggg gatttgataa cagggaaact aagacaagaa ttacaatcag tccattctgg
     1921 ttcaggagtt gggatgattg gtattacctc aattgtatct ggttttaggc aaactataaa
     1981 acataaattt tctcgtaatt gggaaagtat ttcttttatg agtaccggta gaaataatat
     2041 tcctcttgga ataaccggtt acacttatat tccgcgtaat tactatattg cagagaaagt
     2101 tatagaaccc ttacaacttg atagtttagc tgtt
//
LOCUS       DAORHB010000191         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_65345_length_2123_cov_5.212282, whole genome shotgun sequence.
ACCESSION   DAORHB010000191 DAORHB010000000
VERSION     DAORHB010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65345_length_2123_cov_5.212282"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..469)
                     /locus_tag="PKM80_05485"
     CDS             complement(95..469)
                     /locus_tag="PKM80_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93398.1"
                     /translation="MKHYIILILLLSIVLLFGQTKPSDAKSVNYETHNSGLLSLNVYN
                     IGSLSNLHNPYDGMKLMSQSGQWISAKKYRRNNLNQLLYWLSPIPGPGNDQMVTEMDP
                     LWNPNLLVVTDTLSTVAYDGDC"
     gene            complement(480..797)
                     /locus_tag="PKM80_05490"
     CDS             complement(480..797)
                     /locus_tag="PKM80_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93399.1"
                     /translation="MAKYGRLLIIEISLFFLGACQEESLLGPLSESFDPLSIQEDKTP
                     LAGGEYLYQQNIVITNTEDNDFFTYKLITYQGELPVSYMNQTETANALQNLFSNMINN
                     EPL"
     gene            complement(1351..>2123)
                     /locus_tag="PKM80_05495"
     CDS             complement(1351..>2123)
                     /locus_tag="PKM80_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNV93400.1"
                     /translation="PKHIYLYECLGWETPKWVHLPLILNADRTKLSKRMNDVSVDSYI
                     AKGYLKEALINFVALLGWHPSDDRELFTLDELCNAFSLERVNKAGAIFDLTKLDWMNG
                     QYLHSLPVEDIAMRCKHLFLEKGYDLSDENKYQLAIAVARERCTLLNEIVDYCKMFYQ
                     PEELKNDDIKTLLAPEAQKVLAWFATNLPILSAWDTESIQNLVNSGIAELGIKGKKFY
                     TPLRLALIHQQHGPELPSTFIILGKEESIKRLQQYLPS"
BASE COUNT          708 a          377 c          348 g          690 t
ORIGIN      
        1 cgaatttgaa catgctgctg aactcagaga tagaatctgg gaacttcaac agaatctata
       61 atgcatcata ttattgattt aaaaaaagcc caaatcagca atcgccatca taagcaactg
      121 tggaaagtgt atctgtaact accagcaaat ttggattcca aagaggatcc atctccgtaa
      181 ccatctgatc atttccagga ccgggtatgg gagaaagcca atacaataat tgatttagat
      241 tatttctgcg atattttttg gcagaaatcc actgtccgct ttgagacatt aacttcattc
      301 catcataagg attatgcaaa tttgagagag atccaatatt ataaacatta agtgaaagta
      361 aaccggaatt atgggtttca taatttacac tttttgcatc tgaaggtttg gtttgaccaa
      421 acaaaaggac aatggaaaga agtaggatca agattatata gtgcttcatt ttatcctcct
      481 tataaaggtt cattattgat catattgcta aataaattct gtaaagcgtt tgctgtttcc
      541 gtttgattca tataggacac cggaagttca ccttgataag ttattaactt ataagtaaaa
      601 aagtcgttat cctcggtatt ggtaattacg atgttttgtt ggtaaagata ttcaccaccg
      661 gcaagaggag ttttatcttc ttggatgctg agaggatcaa aactttcaga taagggacct
      721 aacaaacttt cttcttggca ggcacccaaa aaaaacaatg aaatctcgat tattaataat
      781 cttccatact tagccataat aattcctttt atactcaata ttctgcattt gttatgatct
      841 acaaaaagca gataagatgt atactatgtg ttctaattac aaaagaaagt cgctgataca
      901 aatgctgagg caactttcta gtataagtat gcgtagattt ttcctatatt tttttccaaa
      961 ctcgccagca aattaatcta tttaaactat aaataacaat ggtatttatg tgaatacaaa
     1021 tattaaaatt ttaagcaact ttttttccca cttctaataa ttacttcgta tatatcactt
     1081 tttaaaaact cgtatgcagc ctatataatt cccatatgat tcccatatca gataagggaa
     1141 tcatacggga tgcttattga ctgtatataa gcttccagta aggagactgc tatcttatat
     1201 aattccttag atttagttga ttagagaata tggtgatggg ctgctgaatt tcgattccgc
     1261 aatttaggga aagagtacat ttagataact ccattatttt tggcaaaaac aaatctgcca
     1321 acttattatc ctccaacaca ttatttgaat ttaactcggc agatattgct gcaggcgttt
     1381 aattgattct tcctttccta aaatgataaa agtggaaggt agctcaggcc catgctgttg
     1441 atgaattaaa gccaaacgta aaggagtgta gaatttttta cctttaatac ccagttcagc
     1501 aattccagaa ttaactaaat tctgaataga ttccgtatcc caagcagata aaattggtaa
     1561 attagttgca aaccaagcaa gaactttttg agcttccgga gctaatagtg tttttatatc
     1621 gtcattcttt aactcctcag gttgataaaa cattttacag taatccacaa tttcgtttag
     1681 taaagtgcat ctttcgcgag ctacagcaat agcaagttgg tatttatttt catcgctaag
     1741 atcatatccc ttctctaaaa aaaggtgttt acaacgcata gcaatatctt caacaggcag
     1801 tgaatgtaaa tattgtccgt tcatccaatc cagcttggtt aaatcaaata tggctcccgc
     1861 tttatttact ctttctaatg aaaaggcatt gcaaagttca tccagagtga aaagttcgcg
     1921 gtcatcggaa ggatgccaac ccagtaaagc aacaaaattg attagtgcct ctttcagata
     1981 accttttgct atatagctat ctacagatac atcattcatc cttttgctca attttgtacg
     2041 gtcagcatta agaataagag gaagatgcac ccatttagga gtttcccaac ccaaacattc
     2101 atacaaataa atatgtttgg gag
//
LOCUS       DAORHB010000192         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_66004_length_2110_cov_3.868127, whole genome shotgun sequence.
ACCESSION   DAORHB010000192 DAORHB010000000
VERSION     DAORHB010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66004_length_2110_cov_3.868127"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKM80_05500"
     CDS             complement(<1..726)
                     /locus_tag="PKM80_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93401.1"
                     /translation="MTEQNIREPRRIISFLSAIDILTLVYCGWIILYMTFGFTRSPEA
                     LKHIPVYLAIFTGVLFLAWLQKQSGWCYSPNNPTKRYRVLNFFRSIYPVLLFGYFYTS
                     GYAFNRIVFRNWLDPFFMSIDKYIFGYLPSLVWGKLYTHWAVQELFHFAYFCYYPMIA
                     GIPIYLYFTKKEAFKEVIFNLTFVFYCCYTIYSILPVIGGRYLPEAMALTKTFRGGPF
                     THIMVFIYRTSNHLGGAFPSSHIA"
     gene            complement(737..1864)
                     /locus_tag="PKM80_05505"
     CDS             complement(737..1864)
                     /locus_tag="PKM80_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV93402.1"
                     /translation="MIDVIPVDNKKQLKEFIMLPFELYKNDSNWVPPLISDQKKFFNP
                     KKNPYYQHSEVKLFLAYKDGKQSGRISAHSNTQHNIEHNDNIGFFGFFECIDDQEVAD
                     ALFEAAYKWNKYRGFISMRGPLNFSVNHDCGLLIKGFDVPPMVMMPYNFSYYQKLYEN
                     YGLTKTMDLYAFYNEYHSIPERITKMADIVSKRNKVEVRSLSYDKKQRKKDIETVFEI
                     YTKAWQYNWGNVPMTKAEFDNIVAELLPIADPDLVFIAEVDGKLAGFSLALPNYNEVL
                     KVMKGKVTPLTVIKALKAKKHINAVRVITMGIVKEFQGRGIDTLLYYHTFKKGLPKGY
                     YRSEFSWVLENNTPMINVAEKLGADIYKTYRLYDKAIEDRI"
     gene            complement(1886..>2110)
                     /locus_tag="PKM80_05510"
     CDS             complement(1886..>2110)
                     /locus_tag="PKM80_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93403.1"
                     /translation="LFAQIEVVIVVRFKQIRFAQYLIVILPVVLFIIFYTLIPNYHKD
                     LPSYFITLLLGNAVIMGILNIIVSKRLGEF"
BASE COUNT          730 a          375 c          361 g          644 t
ORIGIN      
        1 ggcaatatga ctgctgggga aagcacctcc caaatgattg gaagtgcgat agatgaatac
       61 cataatatga gtaaatggac cgccacgaaa tgtttttgtt aaagccattg cttcaggaag
      121 atagcgtcct ccaattacag gaagaatcga atagatagta taacagcaat agaaaacaaa
      181 agttagattg aaaataactt ctttaaatgc ctctttcttt gtaaaataaa gatatatagg
      241 aattccagct atcattggat aataacagaa ataggcaaaa tgaaaaagtt cctgaacagc
      301 ccaatgcgta tataactttc cccaaaccag agaagggaga taaccaaaga tatatttatc
      361 tatactcata aaaaagggat cgagccagtt ccgaaaaaca atccggttga aagcatatcc
      421 tgatgtataa aaataaccaa ataagagcac aggatagatg cttctaaaaa aatttagaac
      481 tctataacgc ttagtagggt tatttggaga ataacaccag ccggattgtt tttgtaacca
      541 agccaaaaaa agtactcctg taaaaattgc cagataaaca ggaatgtgtt ttaacgcttc
      601 cggagagcgt gtaaaaccaa aagtcatata tagtattatc cacccgcaat acaccagagt
      661 gagaatgtct atggcagaaa gaaatgaaat tatccttctg ggttctctaa tattctgctc
      721 tgtcatttag aatccgttat atcctgtctt caattgcttt atcgtaaagc cggtaagttt
      781 tatagatatc tgctccgagc ttttcagcta cattaatcat aggggtatta ttttccagca
      841 cccaggaaaa ttcgctacgg taataaccct tgggaagacc ttttttaaaa gtatgataat
      901 ataaaagagt atctatacct cgtccctgaa attcttttac gattcccata gtgataacgc
      961 gcacggcatt aatgtgtttt tttgccttta gcgcttttat aactgttagc ggagttacct
     1021 ttcctttcat aacttttaga acttcattat agtttggtaa tgctaagcta aaaccagcaa
     1081 gttttccatc tacttcagca ataaagacta aatccggatc ggctatagga agtaattctg
     1141 ccacgatgtt atcaaattca gctttcgtca ttggaacatt tccccaatta tattgccaag
     1201 ctttagtgta tatttcaaag actgtttcaa tatctttttt tcgttgtttt ttatcgtaag
     1261 aaagagagcg gacttccact ttgttccgtt tggaaactat atctgccatt ttagtaatgc
     1321 gttctggaat agaatgatat tcattataga aagcatagag atccatcgtt ttagttaagc
     1381 cataattctc atagagtttt tgataatagg agaaattata gggcatcata accataggag
     1441 gaacatcaaa accctttatc aacagaccgc aatcatgatt tacactgaag tttaagggac
     1501 ctcgcataga aataaaacca cgatacttat tccatttata agctgcttca aaaagagcat
     1561 cagccacttc ctgatcatca atacactcaa aaaagccaaa gaatccgatg ttatcgttat
     1621 gttctatatt atgttgagtg ttggaatgag cagaaattcg tcccgattgt tttccatctt
     1681 tataagcaag aaagagttta acttctgaat gttgataata gggattcttt ttaggattga
     1741 agaacttttt ctgatcacta ataagaggag gaacccaatt ggaatcattt ttatacagct
     1801 caaagggaag cataatgaat tctttcagct gctttttgtt atcaacaggt attacatcta
     1861 tcatcgctga acctcttttg ctaagctaaa attcgcctaa tcttttagat acaattatgt
     1921 tcaatatccc cataattact gcattaccaa gtaaaagagt gataaaatat gaaggcaaat
     1981 ctttatggta attggggata agagtataaa atataatgaa taaaactacc ggcaagatca
     2041 caattaagta ttgggcaaac cgtatctgct taaaacggac aacaataacc acctcaattt
     2101 gtgcaaataa
//
LOCUS       DAORHB010000193         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_66800_length_2095_cov_5.548529, whole genome shotgun sequence.
ACCESSION   DAORHB010000193 DAORHB010000000
VERSION     DAORHB010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66800_length_2095_cov_5.548529"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..652
                     /locus_tag="PKM80_05515"
     CDS             248..652
                     /locus_tag="PKM80_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HNV93404.1"
                     /translation="MPRSIARISIWKKAVPLLVIILSSFLGIASRKFGYILPPFLAKY
                     TGDTMWALAFCALFRMLFTRQRNWEIAIIAFDFSCLIELSQLWHPVWLDRIRSTVFGG
                     LLFGFGFRYIDLFCYAAGCVLGLFIFSLIPEG"
     gene            780..1655
                     /gene="dapA"
                     /locus_tag="PKM80_05520"
     CDS             780..1655
                     /gene="dapA"
                     /locus_tag="PKM80_05520"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNV93405.1"
                     /translation="MLQGTYVALITPFKNGSIDWTALENLIQFHLENGTTGILLLGTT
                     AETSALASDEKDALLRFAMKKIDSKLPVMIGTGTNNLHQTLASTQKAKELGADYALII
                     TPYYIKPTQKGMLEYYGIIASKVDIPIIIYNVPGRTSVNMNAATTVELAKTYPNIVGV
                     KDATGNIVQATQIIRDAPDNFSLMSGEDAMNLPLIAIGGKGTISVTANVAPRLVSEHI
                     STCLQGDFVTAAKQHQYLARLNEMMFIETNPIPAKEALAMMGKINLAFRSPMCPLLET
                     NRAMLKQCLQDYNLL"
     gene            1665..>2095
                     /locus_tag="PKM80_05525"
     CDS             1665..>2095
                     /locus_tag="PKM80_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNV93406.1"
                     /translation="MKQKELIYGFQLIEKREIKEISTTAHLYKHIKSGAELIYYECDD
                     DNKVFMIGFKTVPEDNTGCPHILEHSVLNGSRNFPAKTTFMELVKGSMNTFINAMTFP
                     DMTCYPVASTNDKDFINLMRVYLDAVLFPNIYEQPNILHQEG"
BASE COUNT          671 a          355 c          393 g          676 t
ORIGIN      
        1 ttttaacccg gcgacaagaa gaacgaataa aaaaagtccc agcgggacga cagatactaa
       61 atgaacttta aattattagt cctcttaatc cttaaatcct tgtttctaaa atcaaatctg
      121 tgcgagataa aaatacacac acttcttcct attcaacaaa atgcttgact gaataagata
      181 ggtagtaagc gtaggtttat aatgtatctg gaaataataa aatacgaata ccaaggttaa
      241 aaccgtaatg ccaagaagta tagcgcgtat ttctatctgg aaaaaggcag ttcctttgtt
      301 ggttattatt ttaagttctt ttttgggtat tgcctcccgc aaattcggat atattttacc
      361 tccttttttg gcaaaatata ctggagacac aatgtgggct ttggcttttt gtgctttatt
      421 ccggatgctt tttactcggc aaagaaattg ggaaattgcc ataattgctt ttgatttttc
      481 ttgcttaata gaattgagtc aattgtggca tcctgtttgg ttggatagaa tccgctcaac
      541 agttttcggt ggtttactat ttggcttcgg ttttcgctat atagatcttt tttgttatgc
      601 agcaggctgt gtgttgggtt tatttatttt ttctttaatt ccagaaggtt agtggagatt
      661 tttttacaca aaaaaactgt taaccctatc tttttgattg acggaaatgg tttatttaca
      721 taaaatggaa cagaatttgt attatataaa taaggatata accttggagg ttaaaaaaaa
      781 tgctgcaagg aacttatgtt gccctaatta ctccttttaa aaatggcagt attgattgga
      841 ctgctctgga aaatcttatc cagtttcatc tggaaaatgg aacaaccggt attttgctat
      901 taggaacaac cgcagaaacg agtgctttgg cttctgatga aaaagatgcc ctattacgct
      961 ttgcgatgaa gaaaattgat agtaaactgc ctgttatgat tggaacagga accaataatc
     1021 ttcatcagac cttagcttcc acccaaaaag caaaagaact gggtgccgat tatgctttaa
     1081 ttatcactcc ctattacatt aagcccacac aaaaaggtat gctcgaatat tatggtatca
     1141 ttgcttccaa agtggatatt ccgattatta tttataatgt ccccggtaga acaagtgtaa
     1201 atatgaatgc tgctacaacg gtagaattgg caaaaaccta tcccaatatt gttggagtaa
     1261 aagatgccac aggaaatata gttcaggcca ctcaaataat tagagatgct cccgacaact
     1321 tttctttgat gagtggagaa gatgctatga atcttcctct tattgcgatt ggaggaaagg
     1381 gaacaatttc tgttactgct aatgtagctc cccgtttggt aagtgaacat atttctactt
     1441 gtttacaagg tgattttgtt acagcggcaa aacaacatca atatttagct cgcttaaatg
     1501 aaatgatgtt tattgaaact aatcctattc ctgctaaaga ggcactggcg atgatgggaa
     1561 aaattaattt agctttccgc agtccaatgt gtcctttatt ggaaactaac cgtgcaatgt
     1621 taaaacaatg cttgcaggac tataacctat tgtaagagga acaaatgaaa caaaaagagc
     1681 taatctatgg ctttcaatta attgaaaagc gcgaaattaa agagatatca accaccgccc
     1741 acctctacaa acatataaaa agcggcgctg aactgattta ttatgaatgt gatgatgaca
     1801 ataaggtatt tatgattggg tttaaaaccg ttccagaaga taatactggc tgtcctcata
     1861 tcttggaaca ttcagttcta aatggtagta gaaacttccc tgcaaaaact acttttatgg
     1921 aactggtgaa aggtagtatg aacactttta tcaatgctat gacttttccc gatatgactt
     1981 gttatcctgt agccagtaca aacgataagg attttattaa tttaatgaga gtttacctgg
     2041 atgcagttct ctttcccaat atttacgaac agcccaatat tttgcatcaa gaggg
//
LOCUS       DAORHB010000194         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_69150_length_2051_cov_4.378758, whole genome shotgun sequence.
ACCESSION   DAORHB010000194 DAORHB010000000
VERSION     DAORHB010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69150_length_2051_cov_4.378758"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..469
                     /locus_tag="PKM80_05530"
     CDS             116..469
                     /locus_tag="PKM80_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93407.1"
                     /translation="MVKKHAKITQELELENVEGYTEAQKKYYSSLGFKPYLTENGRIK
                     WLRPDQHLLRINSHLRRSRSARLFHRKNVHFPHRRRHRPAIVKLIQRNWLFILIVIIG
                     LLAVVLVLLNPQIIF"
     gene            484..1254
                     /gene="surE"
                     /locus_tag="PKM80_05535"
     CDS             484..1254
                     /gene="surE"
                     /locus_tag="PKM80_05535"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNV93408.1"
                     /translation="MKILLVNDDGILAPGIRALYKELKNAGHKITIVAPDSERSAASH
                     SLSIRKELKVKKIADNEYAVSGTPVDCSIIALQKILAEPVDIIISGINAGQNMGEDVL
                     YSGTVGAAMEAALLGNRAIAISITSYKNQRFDVAAHWMRKLLDLGIDKQAEAYGVLNI
                     NVPNLPIEEIKGIRLTRTGHRKYYNFVKVIKENDDGFVYEVGGDIPVWDNQTGSDAEA
                     VNNGYISLTPLGFELTKSDTYPRILNWLEENKLLQLES"
     gene            1257..1907
                     /locus_tag="PKM80_05540"
     CDS             1257..1907
                     /locus_tag="PKM80_05540"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNV93409.1"
                     /translation="MRFEDQRLKLVNELKLEGITSPAVLDAFAKVPRENFVLPEYREY
                     SYQNRPLPIKEGQTISQPLMIAIMLQLLDLKSSDTVLEIGTGSGYQSALLAEIVKEVC
                     TVERLESLSLNAQKVLRNAGYNNIYFRIGDGHSGWQKAYPPHKEFDKIIVSAAAQDIP
                     NRLLEQLVIGGKLAIPIGRAGFQILHIVNKTEDGYTITQAGGCSFVPLVCDNNNLG"
BASE COUNT          690 a          310 c          416 g          635 t
ORIGIN      
        1 cgaccaacat caaatgaaaa taaggctcct gtaaaaaaat atgggaatgc caacatattt
       61 ttccgcttga cagaattgat tgtttttata cataatgaaa atcgaggatc agtttatggt
      121 taaaaaacat gctaaaataa cacaagaact tgaactagaa aatgttgagg gatatactga
      181 agcccaaaag aaatattact cttctttagg ttttaagccc tatctaactg aaaatgggag
      241 aataaaatgg ctgcgtcccg atcagcattt attgcgaata aactctcatt tgagaagatc
      301 tcgctcagcg aggctttttc accgtaaaaa tgttcatttc cctcatcggc gaagacatag
      361 acctgctatt gtaaagctca ttcaacgaaa ttggttgttt atcttaattg tgataattgg
      421 gttattagca gttgttcttg tattactaaa tcctcagatt atattttagg agtattaaga
      481 gatttgaaga tattactggt taatgatgat ggaattttag cacccggaat aagagcctta
      541 tataaggaac tgaaaaatgc cggccataaa attacgattg tagccccgga tagtgaaaga
      601 agtgctgcat cgcattcttt aagtattcgt aaagaactga aggtgaaaaa aattgcagat
      661 aatgaatatg ccgttagtgg aactcctgtt gattgttcta ttatagcttt acaaaagatt
      721 cttgccgagc cagtagatat aattatttcg ggaattaatg cgggacagaa tatgggagaa
      781 gatgttttat attctggaac agttggagcc gcaatggaag cagctctttt gggaaatagg
      841 gcaatagcta tttctataac ttcttataaa aaccaaagat tcgatgtggc agctcactgg
      901 atgcgtaaat tgttagattt aggcatcgat aaacaagctg aagcatatgg agttttgaat
      961 attaatgttc ctaatcttcc tatagaagaa ataaagggta tacgtttaac ccggacaggt
     1021 catcgtaagt attataattt tgttaaggtt ataaaagaaa acgatgatgg ttttgtttat
     1081 gaagttggcg gtgatattcc agtttgggat aatcaaacag gatccgatgc ggaagctgta
     1141 aataatggtt atatatcttt aactcctttg ggctttgaac ttacaaaaag tgatacctat
     1201 cccagaattc ttaattggct ggaagaaaat aagttgctgc aattggagag ctgataatgc
     1261 ggtttgagga tcaacgatta aaattagtaa acgagcttaa actggaagga ataacttctc
     1321 cggcagtgtt agatgctttt gctaaagtgc cacgagagaa ttttgtttta ccagaatatc
     1381 gtgaatactc atatcagaat cgtcctttac cgataaaaga gggacaaacc atttcgcaac
     1441 cattgatgat agctataatg cttcaattat tagatttaaa atcatcagat acagttttgg
     1501 agattggaac cggcagtggt tatcaaagtg ccttactagc tgaaattgtt aaagaagttt
     1561 gcactgtaga acgactagaa tcgctttctt tgaatgctca aaaggtttta cgcaatgccg
     1621 gttacaacaa tatttatttt cgcattgggg atggacatag cggttggcaa aaagcatatc
     1681 cacctcataa agaatttgat aagataattg tttctgccgc agcgcaagat atcccaaacc
     1741 gtttactgga acaattagtt ataggtggta agttagcaat tccaattgga agagcaggtt
     1801 ttcaaatatt acatatagta aacaaaaccg aagatggtta tactataact caggcaggag
     1861 gatgttcttt tgttcctctt gtttgtgata ataacaactt ggggtaaaag atgttaaaag
     1921 atgttttcgc ctggcttaaa actataatga aaaaaccgga taaatatgcc gacttaaaac
     1981 tgtttcctgt tttcaatgag tgtagttcat ttgagctata tttacttagt aattatagct
     2041 caaatgaact a
//
LOCUS       DAORHB010000195         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_69236_length_2049_cov_5.667001, whole genome shotgun sequence.
ACCESSION   DAORHB010000195 DAORHB010000000
VERSION     DAORHB010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69236_length_2049_cov_5.667001"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKM80_05545"
     CDS             <1..475
                     /locus_tag="PKM80_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNV93410.1"
                     /translation="FTNANTDAKVLNFAAKLVDLGIQPHTLYKNFYLNQSAEEMRYIG
                     QTLSTIELYHHRQILMMHSTYLMSKENNIDPDSITNVTRWVQGVNGVQVIVYLREDKE
                     GVYKISLRSETIDVNKIAVRYGGGGHKQASGCNMYGDIEEIKAKLLKDIIDACNG"
     gene            459..1331
                     /gene="truB"
                     /locus_tag="PKM80_05550"
     CDS             459..1331
                     /gene="truB"
                     /locus_tag="PKM80_05550"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNV93411.1"
                     /translation="MPVTVNAFLAINKPAGITSFEVIRRLRRITGIRKIGHTGTLDPF
                     ATGLLICCTGSYTRLSCFLEKEEKTYLATMKLGAKTSTGDTEGEIIQTAPVPIFADKL
                     AALEAKALDLAELSVPAYSAVKIQGKRAYELARQGKILELPMRPVKISEFKFLVEEQN
                     APDELTYRCKVSKGTYIRALSEWLAEQIQTLAYTISLKRESIGKITLQDAVALGDLKT
                     DNWTNSQISYERVFPDWTCLKLNDEELYKVINGMDIEFSESSSDIILLLNQDELCALG
                     KKSNNVIHPFIVLK"
     gene            1485..>2049
                     /locus_tag="PKM80_05555"
     CDS             1485..>2049
                     /locus_tag="PKM80_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HNV93412.1"
                     /translation="MNTNSVLTIGTFDGLHLGHQKLLKKVVEIAQAEKLQSVVISYKD
                     HPAFILRAKALPKMLCPITLKQRELAKIGIDRIELLDFTPELAKTPPLKFLKEYVIPT
                     FHPKVIVMGYDSHFGHLRQGNYEFLCRHSQELGYRVEYLEPLLYEGKPISSSTIRNLL
                     SLGKIETANNLLGRAYCLIGTVIHSYSN"
BASE COUNT          690 a          350 c          395 g          614 t
ORIGIN      
        1 ctttaccaat gctaatactg atgctaaagt acttaacttt gctgccaagt tagttgattt
       61 aggaattcaa cctcataccc tttataaaaa tttctattta aatcaatccg cagaggaaat
      121 gcgttatata ggccagacct tatctaccat tgaactctat catcatcgcc agattttaat
      181 gatgcattcc acttatttaa tgagtaaaga aaacaacatc gatccggaca gcataacgaa
      241 tgtaacccgt tgggtgcaag gagttaatgg agttcaggta attgtttatt tacgggaaga
      301 taaagaaggt gtatacaaaa taagtttacg ctcagaaacg attgatgtaa ataaaattgc
      361 tgttcgttat ggaggagggg gacacaaaca ggcatccggt tgtaatatgt atggagatat
      421 agaagagata aaagccaagc tgttaaagga cattatcgat gcctgtaacg gttaatgcct
      481 ttctggcaat aaataaacca gctggtatta cctcttttga agttatcagg cgtctgagaa
      541 gaattacagg aatccgtaaa attgggcata ctggaacttt agatcctttt gcgacaggtt
      601 tacttatttg ttgcacaggt tcttacactc gattatcctg ttttctggaa aaagaggaaa
      661 agacttatct ggcaacaatg aaattgggag caaaaacctc aaccggagac acggaaggag
      721 aaattataca aacagctcct gttcctattt ttgctgataa acttgccgct ttggaagcaa
      781 aagcattaga tttggccgaa ttatccgtcc cagcttattc agctgtaaaa attcaaggta
      841 aacgtgccta tgaattagct cgccaaggaa agatattaga acttcctatg cgtcctgtga
      901 aaattagtga atttaagttc ttagttgagg aacaaaatgc ccccgatgaa cttacttatc
      961 gatgcaaagt tagcaaagga acatatatta gagcactaag cgaatggctg gctgaacaaa
     1021 ttcaaactct tgcttacaca atatctctga aacgagaaag catcggaaag atcactttac
     1081 aagatgctgt agctttagga gatctgaaaa cagataattg gactaattct cagattagtt
     1141 atgaacgagt ttttccggat tggacttgtt taaagctaaa tgatgaagag ctttacaaag
     1201 tgatcaatgg tatggatata gaattttcag aatcaagcag cgatataatt cttctgctaa
     1261 accaagatga gctttgtgct ttggggaaaa aaagtaataa tgttattcat ccttttattg
     1321 tgctaaaatg aacaaagcag ctatattatc tataaacctg caaaatttta ggaacagcaa
     1381 tctattgaat accttcaggt aaattatgaa gttccttaat tcgtttcata aaactataaa
     1441 tcagttaatt agggttgaac gctatatcaa cctattagag gaaaatgaat actaactcag
     1501 ttttaactat tggaaccttt gatggcttgc atttaggcca tcagaagctg ctgaaaaaag
     1561 ttgtagaaat agcccaagca gagaaattac aaagtgtggt tatcagttat aaagatcatc
     1621 ctgctttcat tttaagagcg aaagctttac ccaaaatgct gtgtccgata accctcaaac
     1681 aaagggagct ggcaaagatt ggaatagaca gaattgagct gttggatttt actcctgaat
     1741 tagccaaaac tcccccttta aaattcttaa aagaatatgt tattcccact tttcatccta
     1801 aagtaattgt tatgggttat gattcccatt ttggtcattt gcgtcaggga aattatgaat
     1861 ttctttgccg gcattcccaa gaattaggat atcgagtgga atatttggaa ccacttcttt
     1921 atgaaggtaa acctatcagc agcagcacta tccgaaattt gctttctctt ggcaaaattg
     1981 aaactgccaa taatctattg ggaagagcat attgtttgat cggtacagtt atccacagtt
     2041 attccaatg
//
LOCUS       DAORHB010000196         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_71410_length_2010_cov_3.652685, whole genome shotgun sequence.
ACCESSION   DAORHB010000196 DAORHB010000000
VERSION     DAORHB010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71410_length_2010_cov_3.652685"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKM80_05560"
     CDS             complement(<1..282)
                     /locus_tag="PKM80_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNV93413.1"
                     /translation="MKQYSKIEILEETPVLYSILYLAIPSMLSMLVNILYNVTDTFFI
                     GKLNDPYKVAGVSVALPYYNMLMAIAGIFANGGASYLSRLLGKRDIQTAR"
     gene            complement(489..1739)
                     /locus_tag="PKM80_05565"
     CDS             complement(489..1739)
                     /locus_tag="PKM80_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNV93414.1"
                     /translation="MNLSFKNKKLLGINGLGRIGKLLLWNQIHLRHYDGIVVNCGRVV
                     GKTIDDLIQVIEMDSTYGSIHKFLYGIAGRKAEIKVLDVEEPLLEIEGIPVKILRTAR
                     DPKDVNWLNEGVRIVVDCTGTFLDPTMHTDSGKPCLRGHLDAGALKVICSAPFKLKST
                     NSVPEDATTMIYGINHLDFDPRKHNLISAASCTTTGLAHMLKPLLENETTSRILTASM
                     STIHSATNTQSILDSVPKADATDLRKTRSVLNNIILSTTGVTKALEQVMPNIKHIGFM
                     ADSVRVPTSTVSLINLNVTFHTDLDELGEPLVNRKLINDVYLKAAEGSQKDLLLVSNR
                     QNVSADMMGTMAAVVIEAHETHTRTGFVTIPASTLATHGMVTDKILELPVTHAKIFGW
                     YDNELGSYVNCLSRLTNYVEEHTI"
     gene            complement(1772..>2010)
                     /locus_tag="PKM80_05570"
     CDS             complement(1772..>2010)
                     /locus_tag="PKM80_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNV93415.1"
                     /translation="ECQGLLTSRGGVTSHAAVTATRLGLIGVVNCRDLVVSDLDFNCR
                     IGDTYLKAGDKIALDAISGAIYLGHYPLVSVHCL"
BASE COUNT          635 a          401 c          335 g          639 t
ORIGIN      
        1 cctggctgtt tgaatgtctc tttttcccaa tagacgggaa agatagcttg ctcctccatt
       61 agcaaaaatt cctgcaattg ccattaacat attatagtaa ggcaaggcaa cagatacacc
      121 tgccacttta taaggatcat ttagttttcc tataaaaaag gtatccgtta cattatataa
      181 tatatttact agcatgctca gcatactggg tatggcaaga tacaatatgg aataaagaac
      241 gggagtttct tccaatattt ctattttact atactgtttc atcaaatgtt ttcccttatg
      301 ataatatttt taactatgaa aatatatctc atgaaagaag taaatacctc ctatcatagt
      361 acatttcaag aagtgtttta taatttagga gggtggtaat atgctatata ccaccctcag
      421 tgtaagttag caggcatcaa ttatagccct gttatagtat ctataactga tgcagtgtaa
      481 taactttttt aaatagtgtg ttcctccaca taattcgtca agcgagaaag gcaattcaca
      541 taagagccaa gttcgttatc ataccaccca aaaattttgg catgcgttac aggtagttcc
      601 aatattttgt ccgtaaccat accatgagta gctaaagtgg aagctggaat agttacaaaa
      661 cctgttctgg tatgagtttc atgtgcttca ataactaccg cagccatagt tcccatcata
      721 tctgcagaaa cattttgccg gttactaact agcagcaggt ctttttgtga gccctcagca
      781 gccttaagat agacatcatt aattagttta cgattaacaa gaggttctcc taattcgtct
      841 aagtctgtat gaaaagttac attcaagttg ataagcgaaa cagtgctggt tggaacacgc
      901 acgctatctg ccataaaacc aatatgtttg atattaggca tcacctgctc cagagcttta
      961 gtaacaccag tggtagaaag aatgatgtta ttcaaaacgc ttcttgtttt tcgtaagtct
     1021 gtagcatccg ctttgggtac actatccaga atagattgag tgtttgtagc tgaatgaatg
     1081 gtactcatcg aagcagttaa aatacgggaa gtggtttcat tttctaaaag tggtttcagc
     1141 atatgtgcta aaccggttgt cgtacaggaa gctgccgaga ttaggttatg tttacgagga
     1201 tcgaaatcca aatgattaat accataaatc atagttgtag catcctcagg aacagaattt
     1261 gtcgatttca atttaaaagg agcactgcaa attactttta gagcacctgc atctaaatga
     1321 ccccgtaaac aaggttttcc agaatcagtg tgcattgtag gatcaagaaa agtccctgta
     1381 caatcaacta caattctaac cccttcattc aaccaattca catctttagg gtcacgagct
     1441 gttctcagaa tcttaactgg gatgccttca atttccaata aaggttcctc tacatcaaga
     1501 actttaattt ctgcttttct tccagcaatt ccataaagga acttatgaat agaaccatag
     1561 gtgctatcca tttctataac ctgaataagg tcatcgatgg ttttaccgac aactcttcca
     1621 cagtttacaa cgataccatc ataatgtcta aggtgtatct ggttccataa gagtaactta
     1681 ccaattctac ctaatccatt gattcctagc agttttttgt tcttaaagct cagattcatt
     1741 ttgtttttct ccttatatat gtttttttat tctataaaca atgaacactt actaaaggat
     1801 aatgtcccaa atatatagct ccactgatag catcaagagc aattttatca ccagctttta
     1861 aataagtatc tcctatcctg caattaaagt ctaaatcgga aacgaccaga tcacgacaat
     1921 ttactactcc aattaagccc aaccgtgttg ctgtaactgc tgcatgagaa gttactccac
     1981 ctctggaagt taacaaaccc tgacattcaa
//
LOCUS       DAORHB010000197         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_75500_length_1942_cov_5.196608, whole genome shotgun sequence.
ACCESSION   DAORHB010000197 DAORHB010000000
VERSION     DAORHB010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75500_length_1942_cov_5.196608"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1659
                     /locus_tag="PKM80_05575"
     CDS             376..1659
                     /locus_tag="PKM80_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNV93416.1"
                     /translation="MFACVAVRGISFILPILIIIGSCSFVPLFADILIPMDNTQTNHL
                     KAYGIAFAGLKESLVGRWLLNYRGGSFLFPDSGNLTSLCNIRGVRYENVTSSDVAAIL
                     TEIQANNMESIALEKEPKIAVYIPPNALPWDDAVTLALEYAEIDYDRVWDDEVLAGKL
                     TDYDWLHLHHEDFTGQYGKFYGSYRNTSWYQNDVRVNEAMAAKHGFQKVWQLKHAVAE
                     KIRNYVINGGFMFAMCAATDTYDIALAAKDIDIVDATIDGDGIDPQYQSKLDFSRTFA
                     FENFTLKPNPYEYEFSDIDASDYSRLRGAEADYFQLFDFSAKYDPVPTMLTQCHTNVI
                     DGFLGQTTSFFKDKVKKNVIILAEVPGNNEVKYIHGNIGKGTFTFYGGHDPEDYEHKV
                     GDPETILELHKNSPGYRLILNNVLFPAAEKKQLKT"
     gene            1667..>1942
                     /locus_tag="PKM80_05580"
     CDS             1667..>1942
                     /locus_tag="PKM80_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV93417.1"
                     /translation="MKKFYPNLRSVQIKTGIWTVVIGLILILGYLWLTGHLAGTSQYE
                     LKVSFPDVMGLEIGDKVMYRGMEVGRVKSITAEKDRVITTAKINRSIG"
BASE COUNT          574 a          312 c          410 g          646 t
ORIGIN      
        1 ttttcgccgg gttaaaaccc gtcgttaatg tctgtcgtcc cgctgggact tttactttaa
       61 tacagaactt tccttccaat agggagcttt ttctttcaat ataaaactct tatttctatc
      121 tagatattta tgcttttata ttcgctatca gagttttgac agaatttatg aagttttgaa
      181 tgctacgtcc tctatgtatt tattctttta tattggcttt cagagctttt gactaattaa
      241 agtgtttttc agggtaaaaa aaccagcaac agagatttat cttgacagcg gaacagataa
      301 tttcctttta tggcacagaa atggcttata ataagcaaaa gggaaatgag gaggattaaa
      361 tccttgttta gaaaagtgtt tgcgtgtgtt gctgtaaggg gtatcagctt tattttgccg
      421 attctaataa taattggcag ttgtagtttt gtgcctttat ttgcagatat attaattccg
      481 atggataaca cgcaaaccaa tcacttaaag gcctatggca ttgcctttgc gggtcttaag
      541 gaaagtttag ttggcaggtg gctgttaaat tatcgagggg gaagttttct ctttcctgat
      601 tcaggcaatt taacttcttt atgtaacatt cgtggcgtgc gttatgaaaa tgtaacttct
      661 tccgatgtgg ctgctatttt aacagaaatt caagcgaata atatggaaag cattgcttta
      721 gagaaagagc ccaaaatagc tgtttatatt cctcccaatg cccttccctg ggatgatgca
      781 gttactttag ctttagaata tgccgagatt gattatgacc gggtttggga tgatgaagtt
      841 ttggcaggca agcttacgga ttatgattgg ctgcatttgc atcatgagga ttttacgggt
      901 caatatggca aattttacgg ttcttatcgt aatacaagtt ggtatcagaa tgatgtgcgc
      961 gtaaatgaag cgatggcagc aaaacacggt tttcaaaaag tttggcagct taaacacgca
     1021 gtagcggaaa agattcgtaa ctatgtaata aatggtggtt ttatgtttgc aatgtgtgct
     1081 gctacggata cttatgatat tgctttagca gccaaagata tagatattgt agatgccaca
     1141 atagatggtg atggaataga ccctcaatat caaagtaaat tggatttttc tcgaactttt
     1201 gcctttgaaa atttcacttt gaagcccaat ccttatgaat atgaattttc cgatattgat
     1261 gccagtgatt attctcgttt gcgaggtgca gaagccgatt attttcagct ttttgatttc
     1321 agcgcaaagt atgatcctgt tcccacaatg cttactcagt gtcataccaa tgttattgac
     1381 ggctttttgg gtcagaccac ttcttttttc aaagataagg tgaagaagaa tgtaattatt
     1441 ttagccgagg tccctggcaa caatgaagtt aaatatatcc atggtaatat tggcaaagga
     1501 acattcactt tttatggtgg ccacgatcct gaggattatg aacataaagt tggcgatcca
     1561 gagaccattt tggagttaca taaaaattcg ccaggttatc gtttaatatt aaataatgtc
     1621 ctcttcccag cagcagagaa aaaacagcta aagacataaa aaaagtatga agaaatttta
     1681 tcctaacttg cgtagtgttc agataaaaac cggcatctgg accgtagtta ttggtctgat
     1741 tttaattttg ggttatttat ggctgacagg tcatttagcc ggaacttcac aatatgaact
     1801 taaagtttct tttcccgatg taatgggttt ggaaattgga gataaagtaa tgtatcgcgg
     1861 aatggaagtt ggccgagtga aaagcattac tgccgaaaaa gatagagtta ttactactgc
     1921 caaaataaac cgcagcattg gg
//
LOCUS       DAORHB010000198         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_75557_length_1941_cov_4.986214, whole genome shotgun sequence.
ACCESSION   DAORHB010000198 DAORHB010000000
VERSION     DAORHB010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75557_length_1941_cov_4.986214"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..1394
                     /locus_tag="PKM80_05585"
     CDS             486..1394
                     /locus_tag="PKM80_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV93418.1"
                     /translation="MSKLCKRGKSKEKRDDCPLLELALVVDQDGFPMVSKIYSGNQSE
                     PHTLLEVLTELKDSEDSIDRFTPPAIVMDREIATKDNIHLLEREEYKYFVVERPNSLK
                     EFASEFKSLAGFTESKDNHQGKLYLKKIVSAAKAKVLVYSEARAIKERGKTTSWEQNF
                     LEEINHLNSSIQKGNINKADKIWVKIGRLKEKYGSTATNYEIQLQSNSSDPRRVESLI
                     ITDLHKKSCKSEFPGCYVIETNYTDFSAEEIWKFYMNLNEVESAFRSLKNRIGNKTHS
                     SSERWSDRGTSVLFRISVCYSEKYHL"
     gene            1456..1647
                     /locus_tag="PKM80_05590"
     CDS             1456..1647
                     /locus_tag="PKM80_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93419.1"
                     /translation="MRTDTIFNTTDGYRVQIRQTAQPEEEAKKIYSLLDINIHKNTAT
                     QKFRLLCHQKNNFPYLAIL"
BASE COUNT          695 a          355 c          358 g          533 t
ORIGIN      
        1 gccacaaaag aagaaccaaa acaagtaaca aatgaaactg gaagagtccc tcctttaaga
       61 actgatgtac cttctttttt taggcacttg attagatttt tatacaaaac ttcatcataa
      121 actcctcctt gtttatgtga aacaaagtcc aaaagctcac aaaccgtcag caaccccccg
      181 ctgacggttt ttttttatta cgaaagaaca atgtcctcat cgttttatgg attttatgtt
      241 tattatgaga ttaagaaaaa aacagtgtga accagaaaaa atacagcgga tctttgcctc
      301 taaattacag acagaatata tgaataactc aatcttgaat aagctgttat ctgtatcata
      361 atatgtccag atttataagt gagcttttcc ttggaagtta ttacgatacc attaattgta
      421 tgtcgctaaa atggaataga atttctacta acccaagttt cgcacaaaaa aagtgaaaat
      481 ggctcatcag taaactatgt aagcggggaa agagtaagga aaaacgagat gattgtcctt
      541 tattagaatt agcattagta gtggatcagg atgggtttcc gatggttagt aaaatctaca
      601 gtggtaatca atctgaacct cataccctgc tggaagtatt aaccgaactt aaagatagcg
      661 aagattcaat agatcgtttt acccctccag ccatagtaat ggatcgtgaa atagctacca
      721 aggataatat tcacttgctg gagcgagaag aatataagta ttttgtggta gaaaggccta
      781 attcgttaaa agaatttgcc tctgagttca aatccttagc gggatttacc gaaagcaagg
      841 ataatcatca gggaaagctc tatttgaaga aaattgtcag tgctgccaaa gctaaggttt
      901 tagtttacag cgaagccaga gccatcaaag aaagaggcaa aacgaccagt tgggaacaga
      961 actttctgga agagataaac catcttaaca gttccattca aaaggggaat attaacaagg
     1021 cagacaagat ttgggtaaag atcggtcgat tgaaagaaaa atatggttct acggcaacta
     1081 actatgaaat tcaactccag tcaaacagtt ctgatccacg aagagtagaa tctctgataa
     1141 taactgacct gcacaagaaa tcctgtaagt ccgaatttcc gggttgttat gtaattgaaa
     1201 ccaactatac cgatttcagt gcagaagaaa tctggaaatt ttatatgaat ttgaatgaag
     1261 ttgagtccgc tttccgaagt ttgaaaaaca gaattgggaa caagacccat tcatcatcag
     1321 aaagatggtc ggatagaggc acatctgttc tattccgtat tagcgtatgc tattctgaaa
     1381 agtatcacct ataaactttc tctccaagat gttcatatca gttggcaaag catcaaacaa
     1441 attcttaaag cccatatgcg tactgataca atattcaata ctacagacgg ttatagagtg
     1501 cagattagac aaacagccca acctgaagaa gaagcgaaaa aaatctattc tctattagat
     1561 atcaatattc ataaaaacac agccactcaa aaattcagat tattgtgtca ccaaaaaaat
     1621 aatttccctt atttagcaat actttagcta aaaaaatgcg aaacttgagt taggatttta
     1681 ggattaagag gatttctaat ttaaagttcc gctaggaacg atacatacta acgacgggtt
     1741 tttaacccgg cgataaaaag aatgcctaaa agaagtccca gcgggacgac agatttgaac
     1801 gatgggtttt aacccggcaa aaatcttaag tctactattt tcaaatgact caaacaaaca
     1861 acccctcttc ctctcctcct cctcccgtcc tcaacccatc gttgaggagg agtgaagatt
     1921 gggtagatga caagttgtat a
//
LOCUS       DAORHB010000199         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_77329_length_1914_cov_2.623453, whole genome shotgun sequence.
ACCESSION   DAORHB010000199 DAORHB010000000
VERSION     DAORHB010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77329_length_1914_cov_2.623453"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..681
                     /locus_tag="PKM80_05595"
     CDS             1..681
                     /locus_tag="PKM80_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNV93420.1"
                     /translation="MLVNGFLAAITTDYYMKAIGIKIAQISIQQNKADNYVEIRAKSV
                     RESSIFPQINNVYRVKHNGSYQPLTYTRIIHQKNVQDEVTTTYNHNTAQAVMYRNSDK
                     STLQYSITKNSQDIYSFLAKVISGQVDTGTYPIDANGVSWQANITRYSTEIVDTPLGK
                     YPAVRYEISFQNMTNKKMPYIDMVTFNMLQENNKLNLWVYNRQFAVKATFKKKGISST
                     WELCNIKK"
     gene            678..1034
                     /locus_tag="PKM80_05600"
     CDS             678..1034
                     /locus_tag="PKM80_05600"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNV93421.1"
                     /translation="MKFFRTIYPTLFWLIIIWILSSIPSQDLPSVNIVGFDKLQHLGV
                     YAVLGCLIGYWLRFKNWDIKKIVLIYLGLIVLAGIDEYHQRYIPGRDVSIYDFLANAS
                     GLIIGFLLYLRKYDRS"
     gene            1021..1737
                     /locus_tag="PKM80_05605"
     CDS             1021..1737
                     /locus_tag="PKM80_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV93422.1"
                     /translation="MIEVNKLYVSLNEREILSNINFKLEDSNNLVILGRSGSGKTVLI
                     KTLLGIYYPASGNVIIDGIDIHRADEEQQKATKKRFAMVFQNSALLDSFTVLQNVALP
                     LYERGEKNIAYVRDKVLHCLQIVGLEDTISLYPAQLSGGMRKRISIARALVYDPDYII
                     FDEPVSGLDPITSREIMYYITRIVETASATLITITHELKNIDAIGNQILFLNEGKVIF
                     SGSVKQLYNSTDSFVQQYLK"
     gene            1734..>1914
                     /locus_tag="PKM80_05610"
     CDS             1734..>1914
                     /locus_tag="PKM80_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNV93423.1"
                     /translation="MNIDNKSTLIQLAFFTAIAATIHIVEDLIMRMLPLPFIRVGFSN
                     IVILYLIYHNKILEAF"
BASE COUNT          616 a          330 c          325 g          643 t
ORIGIN      
        1 atgcttgtta atggattttt agcggccata accacagatt attatatgaa agctattggg
       61 attaaaattg cccaaatcag tattcaacaa aataaagctg ataactatgt tgagattagg
      121 gcaaaatctg tgagggaatc atctattttt cctcaaatta acaatgttta tcgcgttaaa
      181 cataatggtt cttatcagcc cttaacctac acccgaatta ttcaccagaa aaatgttcaa
      241 gatgaagtta ccactactta caatcataat actgcacagg ctgttatgta tcgtaattcg
      301 gacaaaagta cccttcagta ttctataact aaaaatagcc aggatattta ttcctttctg
      361 gcaaaagtta tttccggaca agtagataca ggaacttatc ctatagatgc taatggtgtt
      421 tcctggcagg caaatataac tcgctattca actgaaatag ttgatactcc tcttggtaaa
      481 tatcctgcag ttcgctatga gatatctttc cagaatatga ccaataagaa gatgccctat
      541 attgatatgg ttacattcaa tatgctacaa gaaaataaca aattgaattt atgggtttat
      601 aaccgtcagt ttgcagtgaa agccactttt aaaaagaaag ggatttcttc cacttgggaa
      661 ctgtgtaata taaaaaaatg aagtttttcc gaactatata ccccactctt ttctggttga
      721 taattatctg gatactatca tctataccct ctcaagactt gccttctgta aacattgtcg
      781 gttttgataa attgcaacat ttaggcgttt atgctgtact tggatgtctt attggttatt
      841 ggctgcgttt taaaaattgg gatataaaga agattgtcct tatctatctt ggacttattg
      901 tacttgcagg tatagatgaa taccatcaga gatatattcc aggtcgcgat gtatcaatct
      961 acgatttttt agccaatgct tccggattaa ttatcggctt tttgttatat ctgcgtaagt
     1021 atgatcgaag ttaataagtt atatgtttct cttaacgaga gagaaatact atccaacatc
     1081 aatttcaaac tggaagatag taacaattta gttattttag gacgcagcgg cagtggtaaa
     1141 acagttctaa taaaaaccct actgggaatc tattatcctg cttccggaaa tgttattatt
     1201 gatggtatag atattcacag agcagatgaa gaacaacaaa aagccacaaa aaaacgcttt
     1261 gccatggttt tccaaaattc tgcattgttg gattctttca ctgtgctaca aaatgtagct
     1321 ctacctctat atgaacgcgg agaaaaaaat atcgcttatg ttagagataa ggtattacat
     1381 tgtttgcaga tagttggttt agaagatact attagtttat atccagctca gttaagtgga
     1441 ggaatgcgta aacggataag tattgccaga gcattagttt acgatcctga ttatattatt
     1501 ttcgatgaac ctgtttctgg acttgatcct atcacttctc gagaaattat gtattatata
     1561 acccgcattg tggaaaccgc atccgctact ttaataacta tcacccatga attaaaaaat
     1621 atcgatgcta tcggcaacca aattttgttt ttaaatgaag gcaaagttat atttagtggt
     1681 agtgtgaaac agctttataa ttccaccgat agcttcgtac aacaatatct taaatgaata
     1741 tagacaataa atctaccctt atccagttgg ctttttttac tgcaattgca gccactattc
     1801 atattgtgga agatctgatt atgcgaatgt tacctttacc ttttattcgc gttggttttt
     1861 caaatattgt gattctctat ctaatctatc ataacaaaat cctggaagcc ttcg
//
LOCUS       DAORHB010000200         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_79292_length_1884_cov_8.167305, whole genome shotgun sequence.
ACCESSION   DAORHB010000200 DAORHB010000000
VERSION     DAORHB010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79292_length_1884_cov_8.167305"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKM80_05615"
     CDS             complement(<1..920)
                     /locus_tag="PKM80_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93424.1"
                     /translation="MDKERIIDALSLFRTKDTYSAALNFWNTLGYVSDRQPELNSFSF
                     NDFATVSGNKIEPTKARKDDWDKFYLLFQITDEEMKNHFKQNSQVIIPGIAKQRFQAT
                     NIKSYLFASLKLKGDKYTRTQLANIARQINKQYAIPLIILFKYNNLVTIAVVNRRQNL
                     IDQQKDVLEKVTMIKDIKTTDTHRAHIDILADLSLEKLSEKYNINNFDTLHNAWAATL
                     DINELNKRFYKELSNWYFWAIKKVVFPAGDEENEEVRNSIGLIRLLTRLIFVWFMKEK
                     GLIPDALFDPQQIKNIIKFEDFNDSAYYKA"
     gene            complement(931..>1884)
                     /locus_tag="PKM80_05620"
     CDS             complement(931..>1884)
                     /locus_tag="PKM80_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNV93425.1"
                     /translation="QIDIIIATDCISEGQNLQDCDYVINYDIHWNPVRIIQRFGRIDR
                     INSPNTKIQMHNFWPTDDLDKYIKLKYRVEARMALVDLTATGADNILKDEDIDNWLEN
                     DMKFRTKQLKRLKEEVIDLEDMEDSISLTDFTLNDFRIDLLNYLKANEIELRDAPLGL
                     YAITPCPDNAFWLKNNALSEEQRQIIRPGIIFCLKHFEDGSEYNKLNPLQPYFLVYIR
                     DDGTVRYHYTNVKQILEIYRLLTKDRKEAIKELCDLFDKETQEGQNMDKYNTLLIKAI
                     EDIKNSLNKRTNQQLQGNRDALIPKKPQEQSFELITWLIIK"
BASE COUNT          564 a          315 c          307 g          698 t
ORIGIN      
        1 atagctttgt aataagcgga atcattaaaa tcttcaaact tgataatatt ttttatctgc
       61 tgtggatcaa aaagggcatc aggaataagg cctttttctt tcataaacca aacaaaaata
      121 agacgagtta gcaatctgat aagaccgatg gaatttctta cttcttcatt ttcttcatca
      181 cctgctggaa aaacgacttt cttaattgcc caaaaatacc aattggacag ttctttataa
      241 aatcttttat ttaattcgtt aatatccaaa gtagcagccc aggcattgtg taaagtatca
      301 aagttgttga tattatactt ttcagatagt ttctctaaag aaagatcagc taaaatatca
      361 atatgagctc tgtgagtatc agttgttttg atatccttaa tcatagttac tttttccaaa
      421 acatcttttt gctggtctat caaattttgc cttctattaa ccactgcaat agtaacaaga
      481 ttattatatt taaagagaat aatcaaaggg atagcgtact gtttattgat ttgccgggct
      541 atatttgcga gctgagttct ggtatattta tctcctttaa gcttaagaga agcaaaaaga
      601 taggacttta tgttagtggc ttgaaatctc tgtttggcaa ttccaggtat aattacttga
      661 gaattttgtt taaaatggtt tttcatttct tcatcggtaa tttgaaagag cagataaaac
      721 ttatcccaat catcttttct ggcttttgta ggttctattt tattgccgct aacagtggca
      781 aaatcattaa aactgaaact atttagttcc ggttgtcggt cactaacata accaagggta
      841 ttccagaaat tcagagcagc agaataggta tctttagttc tgaaaagaga cagggcatca
      901 ataattcttt ctttatccat tataattcct ttatttaata atcaaccagg tgattagttc
      961 aaaactctgt tcttggggtt tcttgggaat gagggcatct ctatttcctt gcaattgttg
     1021 attggtgcgt ttgttcaaag aattttttat gtcttcaata gctttaataa gcaaggtatt
     1081 atatttatcc atattttgtc cttcttgggt ttctttatca aagaggtcac aaagttcctt
     1141 tattgcttct tttctatctt tagtcagcaa tctgtaaatt tccagaattt gttttacatt
     1201 ggtgtaatgg taacggacag ttccatcgtc tctgatatat acaaggaaat atggctgcag
     1261 gggatttaat ttattatatt ctgagccatc ttcaaagtgc tttaaacaaa agattatgcc
     1321 gggtcggata atttgtcttt gctcctcaga taatgcatta ttttttagcc agaaagcatt
     1381 atccggacag ggagttatgg catataaacc caagggagca tcgcgtaatt caatttcgtt
     1441 agcttttaaa taatttagca ggtctattct aaaatcattt agagtaaaat cagttaagct
     1501 tatgctatct tccatatctt ccagatcaat tacttcttcc tttaaccgtt ttagttgttt
     1561 tgttctaaac ttcatatcgt tttctaacca attatcaata tcctcatcct ttaggatatt
     1621 atcagctcca gtggcagtta aatcaaccaa agccattctt gcttctactc tatatttcag
     1681 ttttatgtat ttatccaaat cgtcagtggg ccagaaatta tgcatttgga ttttggtatt
     1741 gggactattg attctgtcta ttctgccaaa gcgttgaata atacgaaccg gattccagtg
     1801 aatatcataa tttattacat aatcgcagtc ctgtaaattc tgtccttcgg aaatgcaatc
     1861 ggtagcaata attatatcaa tttg
//
LOCUS       DAORHB010000201         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_82291_length_1841_cov_5.426652, whole genome shotgun sequence.
ACCESSION   DAORHB010000201 DAORHB010000000
VERSION     DAORHB010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82291_length_1841_cov_5.426652"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1442)
                     /locus_tag="PKM80_05625"
     CDS             complement(231..1442)
                     /locus_tag="PKM80_05625"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNV93426.1"
                     /translation="MKNILTNVINPVSCEVIQFLPEQVISIEGSKITAIIPKQEFQGI
                     VDEDLSHQFALPGFIDLHIHLSQYYIRGLYEPALLPWLNKYVFPEEARSKDNEYAQKL
                     SRDFFSALLKDGTTTAVIYTAPFFEACNTAFEIASEIGIRALIGMTLMDKNCPEPLKQ
                     DSHKAIEDSFLLYEKWHNKNAKLDYVFTPRFAPTCSMELMQEIGKFAKKHNAWIQTHL
                     SENKAELNLVKELFGFDNYTLVYEKAGLLSEHSIFAHCIHLDDSELQLLAENKCKIAH
                     CPDSNFFLKSGEFPIKRIEEKGIAYGLGSDVGSGTTINMLYHTKMMNYRQSWEPVSAA
                     KALYYITLGSARLLGLDSEIGSLETGKEADIVFIEPAEDFLVDDNIISKLVFCESDFT
                     ITHTLVGGKEL"
     gene            complement(1445..1591)
                     /locus_tag="PKM80_05630"
     CDS             complement(1445..1591)
                     /locus_tag="PKM80_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HNV93427.1"
                     /translation="MEVTIKRPFHKQKTLIRELCSAKGTGSQNAPELHFGINKGKVVN
                     IRIK"
BASE COUNT          553 a          348 c          301 g          639 t
ORIGIN      
        1 acaacctata tatctataca acagttcgtt ataacttatc cacatcgttt ttttgcggga
       61 atgcctagaa agtaggaagc ccatcccata gactataaat tttccatcct ttatatagtg
      121 tagctatatt atttatcata gaacggtttt acaagacagc aaaaatagta cttaactgct
      181 ttgattcata tttatggctc ttctgctatt tcgggaaccg aaaaaaacat ctaaagttct
      241 ttgccgccaa caagtgtatg ggtaatagta aaatcacttt cgcaaaagac aagtttagaa
      301 attatattat cgtctacaag aaaatcttcg gctggttcga taaagactat atcggcttct
      361 tttcctgttt ccaaagagcc tatttcgcta tccaaaccaa gtaatcttgc actgcctaaa
      421 gttatataat aaagtgcctt agcagctgaa acaggttccc agctttggcg gtaattcatc
      481 atttttgtat gatataacat atttattgta gttcctgatc ctacatcgct tcctaaaccg
      541 tatgcaattc ctttttcttc aattcttttt attgggaatt ctccactttt cagaaagaag
      601 ttggaatctg gacagtgagc tattttacat ttattttcag caagtaattg taattcacta
      661 tcatctaaat gaatacagtg agcaaagata ctgtgttcgc taagcagacc agctttttca
      721 taaaccagag tataattatc aaaaccgaac agttctttca ctaagttcaa ttcagctttg
      781 ttttccgaga ggtgagtttg aatccaggca ttatgttttt tagcaaattt accaatctct
      841 tgcattagtt ccattgaaca ggttggggca aaacgaggag taaatacata atcaagttta
      901 gcatttttgt tatgccattt ttcataaagg aggaaactat cttcaatcgc tttatggctg
      961 tcttgtttaa gaggttccgg acaattttta tccatcaagg tcattccaat tagagcccgg
     1021 attcctattt ctgaggcaat ttcgaatgct gtattacagg cttcaaaaaa aggtgcagta
     1081 taaattactg cagtagttgt tccatctttc agcaaggcag aaaaaaaatc tctactaagc
     1141 ttttgggcat attcattatc tttagaacga gcttcttccg gaaaaacata tttatttaac
     1201 caagggagta atgcgggctc atataaaccg cgaatataat attgacttaa atgaatgtgc
     1261 aaatcaatga aacctggtaa agcaaattga tgggataggt cttcatctac aattccctga
     1321 aattcttgtt tgggaattat agccgttatt ttactacctt caatactgat aacttgttct
     1381 ggtagaaatt gtatcacttc gcaggaaaca ggattgataa cattggtcag gatgtttttc
     1441 atatttactt tatccttatg ttaactactt ttcctttgtt tataccaaaa tgcaattccg
     1501 gagcattttg agagcctgtt cctttagcac tgcaaagctc cctgattaat gttttttgct
     1561 tatgaaaagg tctttttata gttacttcca ctcttgttcc aaaagcgggg gtattgggaa
     1621 ctttatcata attatttgta tcggttacca gctctacgat atcatttttc caaactggtt
     1681 ttacataaag agcgttatta ttggttactg ttttgttttg taatatctta gctccattac
     1741 cactccaacc acaagatcaa ttaacccatc cctgtttaaa tcagcagagg catttcccca
     1801 accgttataa actcgtgctc ccgaaagcca ggttatatct t
//
LOCUS       DAORHB010000202         1832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_82964_length_1832_cov_4.094541, whole genome shotgun sequence.
ACCESSION   DAORHB010000202 DAORHB010000000
VERSION     DAORHB010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82964_length_1832_cov_4.094541"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKM80_05635"
     CDS             complement(<1..528)
                     /locus_tag="PKM80_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV93428.1"
                     /translation="MQDFVAAKFEMAEYWHINSGLEERVKEFLAKKIEPDVVLQIDIP
                     EKDFNIAGRGSSELKNLLKRLGVDSDILRRIAVASYEAEINVAAHSIGGKMISNVYDD
                     LIYMQFIDNGPGISDIEQAMIPGYSTADDLVREFGFGAGLGLPNIKKNSDALHIESAL
                     GSPTLVEIIIFFWKSW"
     gene            complement(500..847)
                     /locus_tag="PKM80_05640"
     CDS             complement(500..847)
                     /locus_tag="PKM80_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNV93429.1"
                     /translation="MTLYDIVELLDAEVLYEGADLNKVVPCAFASDLISDILMCTKEP
                     TLLLTGLTNNQVIRLADMIDLIGIVFVRGKKPMQDVIDMAKERGLPLIATKMTLYRSS
                     GILYNAGLRSCKI"
     gene            1285..>1832
                     /locus_tag="PKM80_05645"
     CDS             1285..>1832
                     /locus_tag="PKM80_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNV93430.1"
                     /translation="MELQYFGHSTFLFTNSQKQNIIIDPYLNANPLSPVKSNQVKADY
                     IILTHAHSDHMADALTIADRHKTTIICVSELASIISKSGFRTHPLQIGGAFRFDFGRV
                     KFVPAFHGSSTMNGVYAGLAAGAVFTVEGKTIYHCGDTGIFGDMKLIGEIDKIDILLI
                     PIGGNYTMDIDDAVHAVTMINP"
BASE COUNT          622 a          344 c          285 g          581 t
ORIGIN      
        1 ccaactcttc caaaaaaata ttattatttc taccaaagta ggtgaaccca aagctgattc
       61 aatatgcaag gcatcgctgt ttttctttat attaggtaaa cctaaaccag ctccaaaacc
      121 gaattcacga actaaatcat ccgctgtaga atatccggga atcattgcct gttcaatatc
      181 gctaattccg ggaccattat ctataaattg catataaatc aaatcatcat atacattact
      241 aatcatttta ccacctatag aatgagcggc aacatttatt tcagcttcat aactagcaac
      301 tgcaattctt cgtaaaatat cagaatctac tcctaagcgt tttagtaggt tttttagttc
      361 agaagaacct cttccagcaa tattgaaatc tttttcaggt atgtctattt gcaaaactac
      421 atccggttcg attttttttg ctaaaaattc cttcacccgt tcttctaaac cggaattaat
      481 atgccaatat tcagccatct caaattttgc agctacgaag tcctgcatta tataaaattc
      541 cactggatcg atatagagtc attttagtag ctattaatgg aagcccgcgt tccttagcca
      601 tatcaataac atcttgcatt ggttttttcc cacgaacaaa aacaatacct atcagatcaa
      661 tcatatcagc aagacgaatt acctgattat ttgttaatcc tgttaaaagt aaagttggtt
      721 ctttagtgca catcaggatg tccgaaatta aatctgaagc aaaagcacaa ggaactactt
      781 tattcaaatc agctccttca tatagaacct ctgcatccag aagttcgaca atatcatata
      841 aggtcattca ttctttccct taatcaattt ataataaatt cagtaaatat agatattgaa
      901 attcagtcaa gaaaaaaaga tgaattaaag tatgataaat tcataactaa atagacaaag
      961 atataatatt gaactaagaa tctattttac agcgcattag caccaagctc cctgtaccat
     1021 atatgaatca taatgttact aagttcttcc ttagttgcta tcccggagct gtggtgctgt
     1081 ctcttgggaa ttttccacaa taataccacc gcaactctac gataacaagc aaggaatagg
     1141 caaggaacaa gcaatggtta tttattatta aataataaaa ggatgacgat atttacgcaa
     1201 ttacataacg atataatgaa gatttgcttt tggaggtaaa tcgattataa tattaataaa
     1261 aacagaagga aggagagtaa taaaatggaa ctgcagtatt ttggacattc cacctttctt
     1321 ttcacgaaca gccaaaagca aaatattatt attgatcctt atttaaatgc taatccccta
     1381 tctccggtaa aatccaatca agtaaaagca gattatataa ttttaactca cgctcattca
     1441 gatcatatgg ctgatgcctt aactattgca gataggcata aaaccactat tatttgtgta
     1501 tctgaacttg cttccatcat ttccaagagt ggatttcgta cccatcctct gcaaataggg
     1561 ggtgcttttc ggtttgattt tggtagagta aaattcgttc ctgcttttca tggttcctcg
     1621 acaatgaacg gagtttatgc aggactagca gctggagctg tctttaccgt tgagggaaaa
     1681 actatatatc attgtggtga tacaggcatt tttggagata tgaaactgat tggcgaaatt
     1741 gataagatag atatattact aattcctatc ggagggaatt atactatgga tattgatgat
     1801 gccgtacatg ccgtaactat gattaaccca ga
//
LOCUS       DAORHB010000203         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_83493_length_1825_cov_4.180791, whole genome shotgun sequence.
ACCESSION   DAORHB010000203 DAORHB010000000
VERSION     DAORHB010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83493_length_1825_cov_4.180791"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKM80_05650"
     CDS             <1..522
                     /locus_tag="PKM80_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNV93431.1"
                     /translation="IVIFSFIGLSIGVQPLIGFNYGAQNYGRMKKIILTANLICLAMA
                     AFFTIFFAIFPGELIAVFIKDISIIAIGKMILEAYVFAIPFAGVGMILMATLQSMGKA
                     LPAFIVSLSRQGLVYIPMLFLLNHLFQFNGLIFAMPIADVITTILSFIFVYHITKQLK
                     TSLDTKPSFLIRD"
     gene            556..>1825
                     /gene="rocD"
                     /locus_tag="PKM80_05655"
     CDS             556..>1825
                     /gene="rocD"
                     /locus_tag="PKM80_05655"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNV93432.1"
                     /translation="MSTNLKYGNISSREAIELEEKYGAHNYHPLPVVIAKGEGVFVWD
                     PEGNRYYDFLSAYSAVNQGHCHPKIIQALIDQAKVLTLTSRAFYNNKLGEYEKYITDY
                     FGYDMVLPMNSGAEAVETAMKLARKWAYDVKGVTPDEAILIFAENNFHGRTISIITAS
                     SDPDCFEGFGPFTPGITRVPYDDADALKEYLEKNGKNVAAFIVEPIQGEAGVVVPKDG
                     YLKECYNLCKEHNVLFIADEIQSGLARTGKLLACDYDNVRPDVLILGKALSGGVLPVS
                     AVLADKDIMLLIKPGQHGSTYGGFPLACAVAVASLQVIKEEKLADRAAELGEYFRLEL
                     GKIKHPMLKLIRGKGLLNAIVIEPKNGFEAWDVCLRLKEKGLLCKPTHRHIIRLAPPL
                     IITKEQLDECVQIIKTVFDELQNHRPGAGPL"
BASE COUNT          553 a          291 c          362 g          619 t
ORIGIN      
        1 atagttattt tttcttttat tggtttatcc ataggtgtac aacctcttat tggattcaat
       61 tatggagctc aaaattatgg taggatgaaa aaaataatat taaccgccaa tttgatctgt
      121 ttagctatgg ctgctttttt cactatattt tttgctatct ttcctggtga acttattgca
      181 gtatttatta aagatatatc gattatagca atcggaaaaa tgattttgga ggcctatgtg
      241 tttgctattc cttttgcagg agtgggtatg attttgatgg caactcttca atctatggga
      301 aaggcattgc cagcatttat tgtttcgctt tctcgtcagg gccttgttta tattccgatg
      361 ctgtttttat taaatcatct attccagttt aatggtttaa tatttgcaat gccaattgcc
      421 gatgttataa ctacgatact ttcatttatt tttgtgtatc acataaccaa gcagctaaaa
      481 acttcccttg acacaaaacc ttctttttta ataagggatt aagatataag aaaataaaat
      541 aaatatggag gaaaaatgtc cacaaatttg aaatacggaa atattagcag tcgtgaagca
      601 attgaactgg aagagaagta tggagctcat aattatcatc ccctacctgt agtaatagct
      661 aaaggggaag gtgtttttgt ttgggatccg gaaggaaacc gatattatga ttttctttct
      721 gcatattcag cagtaaatca gggacattgt catcccaaaa ttatccaagc attaatagat
      781 caagccaagg ttcttacttt aacatcacgt gctttttaca ataataaact gggtgaatat
      841 gagaagtata taaccgatta ttttggttat gacatggttt tacctatgaa ttctggcgct
      901 gaggcagttg aaacagcaat gaaacttgct cgtaaatggg cctatgatgt taagggtgtt
      961 actcctgatg aagctattct tatttttgct gaaaacaact ttcatggcag aacaatatca
     1021 ataattactg catcttcaga tccggattgc ttcgaaggtt tcggtccttt tactcctggt
     1081 attactcggg ttccttatga tgatgctgat gcgttaaaag aatatttgga aaaaaatggt
     1141 aagaatgtag ctgcttttat tgtagaaccc attcaaggtg aagcaggggt agttgttcct
     1201 aaagatggtt atctaaaaga atgttataac ctttgtaaag aacataatgt gctgtttata
     1261 gcagacgaaa ttcaaagtgg tctagccaga actggtaaac ttttagcttg cgattatgat
     1321 aatgtgcgtc ctgatgtttt aattttaggt aaagcacttt ccggtggagt attacctgtt
     1381 tctgctgttt tagccgataa agatattatg ctgctaatta aaccaggtca acatggttca
     1441 acttatggtg gttttccact tgcctgcgca gtagcagtcg cttctttaca agttattaaa
     1501 gaggaaaaat tagcagaccg agcagctgaa ttaggagaat atttccgtct agagctaggt
     1561 aaaataaagc atcctatgct aaaacttatt agaggtaagg gcttattaaa tgcaattgtt
     1621 attgagccta agaatggttt tgaagcctgg gatgtttgtt tgagattgaa agagaagggt
     1681 ttattatgca aaccaactca tcgtcatata attcgtcttg ctccaccttt aattattact
     1741 aaagaacaat tggatgaatg tgttcagatc atcaaaacag tctttgatga actccagaac
     1801 caccggcccg gcgcgggacc gctca
//
LOCUS       DAORHB010000204         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_83718_length_1822_cov_5.363328, whole genome shotgun sequence.
ACCESSION   DAORHB010000204 DAORHB010000000
VERSION     DAORHB010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83718_length_1822_cov_5.363328"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PKM80_05660"
     CDS             complement(<1..1096)
                     /locus_tag="PKM80_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV93433.1"
                     /translation="MKTNTQDIINRINLQAQKARKAILTMTTLAGSGHPGGSMSSIDL
                     LLCVYNTIKHNPAQPKLPDRDRVVVSNGHISPAVFSTLGIMGYFDLDDAIAEFRLTGS
                     MFEGHIERNVPGVEWSSGNLGQGLSAGTGFALASRINKTPYNVYVFMGDGEQQKGQIS
                     EARRFATKYNLDNIIAIIDYNQLQISGSIHNVMPQNIRKNWEADGWKVIEVNGHDVAL
                     VLDALEKARSFQQPTMILAHTVMGKGVSFMQNNAKYHGSTLDETQLNNALKELGEENE
                     LKRYRKLRMDFKPEKNIPMRFTFTPDIKTGNPIRYETDSDCRSAWGKAIADLVKNNAA
                     AKTPIVVLDCDLATSVKTSEFAEEKPENFFQ"
     gene            complement(1175..1250)
                     /locus_tag="PKM80_05665"
     tRNA            complement(1175..1250)
                     /locus_tag="PKM80_05665"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1215..1217),aa:Ala,seq:ggc)
BASE COUNT          577 a          344 c          310 g          591 t
ORIGIN      
        1 attggaaaaa attctctggt ttttcttcgg caaattcact ggttttaaca ctggtggcta
       61 aatcacaatc taaaacaaca attggtgttt tagcagctgc attatttttt accaaatctg
      121 ctatagcttt accccaagca cttctacaat cgctatctgt ttcataacga atagggttgc
      181 ctgtttttat atccggggta aaagtgaagc gcataggtat atttttttca ggcttaaaat
      241 ccattctcaa tttacgatat cttttcagtt cattctcttc acccagttct ttcaaggcat
      301 tatttagctg tgtttcatcc aaagtggaac catgatattt agcattattt tgcataaatg
      361 aaacaccttt acccataacc gtgtgtgcca gaatcatcgt gggctgttgg aaactgcgtg
      421 ccttttccag agcatcaaga actaaagcaa catcgtgacc atttacttca attactttcc
      481 agccatcggc ttcccaattt tttcgtatgt tctgaggcat aacattatga atagaaccgc
      541 ttatctgaag ctgattatag tcgattattg caattatatt atccagatta tattttgtgg
      601 caaagcgtct tgcttcacta atctgacctt tttgctgttc tccatcgccc ataaaaacat
      661 aaacattata aggggtctta tttattctgg atgcaagagc aaagccagtt ccggcagata
      721 aaccctggcc caaattgcca ctgctccatt caactcctgg aacatttcgt tctatatgac
      781 cttcaaacat actaccagtt aagcgaaatt ccgctatagc atcatccaga tcaaaatatc
      841 ccattattcc caaagtgctg aaaacagcag gagaaatatg cccattgcta actacaactc
      901 tatctctatc aggcagttta ggttgagcag gattatgttt gatagtgtta tacacacata
      961 aaagaagatc tattgaagac attgaaccgc caggatgacc tgaaccagca agagttgtca
     1021 ttgttaatat tgccttacgt gctttttgtg cttgaaggtt aattctatta attatatctt
     1081 gagtattggt tttcatttgt tattccttat ttataatatc atttgcaaaa agaattaaat
     1141 ccatcaccaa atatttgttg gtaaaggaaa aaagtggtgg agcatagcgg gatcgaaccg
     1201 ctgacctcat gactgccagt catgcgctct cccagctgag ctaatgcccc aaaaagagat
     1261 tcacaatatg tgataacatc tctggaaaag ggcagaattc tgtcaagcag aaactacgat
     1321 tttctcgaat aagatatttc aacatattgg tatataacaa gttgatttac agcttttttc
     1381 ctgtttgatt ttattgtgtt gtatgtaata gagagaaaat actacttcag ttatccaaaa
     1441 acagcaggat tggaaaaaat gtacttctac tacttgtatt caagctaact tatataatac
     1501 taaatgacac actggaaaaa tagccataac ttaggcgaat tctaacaaga tcttggttat
     1561 aaatcaattg ctaacagaaa aaatagactc cttttattta aatttaggat taagtagaaa
     1621 aagagacagt atctacttgt ggtataatct tttcactttt ctgtcttcca atccactcat
     1681 aattatctat tgaacctcca ttatgcgagt atatttttag taagaatgat tgtaagaggg
     1741 gagcaaagga atagggtgta agggaaagaa ggcattccca caaaaaagtg atgtggacaa
     1801 gttataacac actgttgtat ag
//
LOCUS       DAORHB010000205         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_88147_length_1766_cov_5.338399, whole genome shotgun sequence.
ACCESSION   DAORHB010000205 DAORHB010000000
VERSION     DAORHB010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88147_length_1766_cov_5.338399"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKM80_05670"
     CDS             <1..705
                     /locus_tag="PKM80_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93434.1"
                     /translation="TQYLCKTFLVISLTIMLFVSACICVRKDNIEQGVKVNGVTLNYE
                     NEVTLSEGYDSNFLTIDGETLSLNIKGSSEPKINLQLKYWEYKPGDAKFSLKKEKLSY
                     ETKSGKPVSLFSITGSIPKNLNLNISTGTGKVNLSDMIGEQTITIQAGTGSIIVSESK
                     LKKLSAETGTGSIIIDKCNIDTTLLESGTGSVHMKKSNIDMASVNTGTGNIILENSNI
                     NKQYFETGTGKVIINK"
     gene            complement(921..>1766)
                     /locus_tag="PKM80_05675"
     CDS             complement(921..>1766)
                     /locus_tag="PKM80_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNV93435.1"
                     /translation="TNVLSIADSALSIADKEMVNLLIFDTAGRLHIDEPMMEEVARLK
                     AHIKPDYIFFVADATTGQDAVNIAKEFYDKLAFDAVILTKMDGDARGGAALSIKAVTG
                     KPVAFVGIGEKISDLEIFYPDRMASRILGMGDVLTLIEKAEESIDMEEEEKLARKMLK
                     NQFTLNDFLKQLQSIKKMGSLESIIRMIPGLGHKLPADLNIDEKALKHIEAIIQSMTE
                     KEREHPEIINGSRRLRIAKGCGRSVTEVNRLLKQFDEMKKMMKKVSTPKGMKHLERMM
                     GGKLQ"
BASE COUNT          552 a          317 c          305 g          592 t
ORIGIN      
        1 acccagtatt tatgtaaaac ctttttagtg atatcgttaa ctataatgct atttgtttca
       61 gcgtgtatct gtgtaagaaa agataacatt gaacaaggtg tgaaggttaa tggagttact
      121 ttgaactatg aaaatgaagt aactctttct gaaggatatg attctaattt tctaacaata
      181 gatggagaaa ctctttcttt gaatattaag ggaagctctg aaccgaaaat taacttacag
      241 ctaaaatatt gggaatataa acctggtgat gcaaaattta gcttgaagaa agaaaaatta
      301 agctacgaaa ctaaatctgg aaagcctgta tctttgttca gtataactgg aagtatacct
      361 aaaaacctga atctcaatat ttctacggga acaggaaaag taaatttgtc agatatgata
      421 ggagagcaaa ctattacaat tcaagctgga accggaagta taatagtttc cgaaagcaag
      481 cttaaaaagc tctctgcaga aacaggaaca ggatcaatca taatcgataa atgcaatatt
      541 gatactactt tgctggaatc tggaacaggt tctgttcaca tgaaaaaaag caacatagat
      601 atggcttcag tgaatacggg gactggaaac attatcctgg aaaatagtaa tatcaataag
      661 caatattttg aaacaggcac aggtaaggtg attataaaca aatgaacaaa atatgaaatg
      721 atgataggga gatttggtat atagggaagc gataagttat attagttgag attcattctt
      781 caaatcccta tagtctatat aatcctgttc ttctcacagc taatttttta tcgccactaa
      841 gctctctggt gaaggaattc ttttaattcc aggcttattt aatttgtgta atccttgtga
      901 tcctgtcctc tttttggtaa ttactgtaat tttccgccca tcattctttc caaatgtttc
      961 attcccttcg gtgtgctcac ttttttcatc atttttttca tttcatcaaa ttgttttaag
     1021 agtcgattaa cttctgtaac gcttcgtccg caaccttttg ctattctcaa tcttctgcta
     1081 ccattaataa tttctggatg ttcgcgttct ttttctgtca tcgattggat aattgcctca
     1141 atatgtttca gtgctttctc atcgatattc agatcagcgg ggagtttatg tcccaaacct
     1201 gggatcattc tgataatgga ttccagcgaa cccatttttt taatgctctg caattgtttc
     1261 aggaagtcgt ttaaggtaaa ttggtttttc agcattttgc gggcaagttt ttcttcctct
     1321 tccatatcta tagattcttc cgctttttcg atgagggtta aaacatcacc cattcctaaa
     1381 atacggcttg ccatacggtc ggggtaaaaa atttccagat cgcttatttt ttctccgata
     1441 ccaacaaaag caacaggttt acctgtaaca gctttaattg ataaagctgc tcctccgcgt
     1501 gcgtcgccat ccatttttgt taaaatcaca gcatcaaaag ctagtttatc ataaaattct
     1561 ttggcaatat taacagcatc ctgtcctgta gttgcatctg caacaaaaaa gatataatct
     1621 ggttttatat gagctttcaa acgcgcaact tcttccatca tcggttcatc gatatgtaaa
     1681 cgtcctgccg tatcaaaaat aagaagattt accatttctt tatcagctat agataaagcg
     1741 gaatctgcaa tgctcaggac atttgt
//
LOCUS       DAORHB010000206         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_93904_length_1700_cov_2.793313, whole genome shotgun sequence.
ACCESSION   DAORHB010000206 DAORHB010000000
VERSION     DAORHB010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93904_length_1700_cov_2.793313"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PKM80_05680"
     CDS             complement(<1..1424)
                     /locus_tag="PKM80_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNV93436.1"
                     /translation="MRKMFSLLLIILAAGMLNALPKFIIGIPASELSLSKEENSLRNK
                     VKELSAQDLEIYYYNEFYIIAGTNVSNYPKAVNLGTPQDGRFYLVTIGNRHPDFDRNI
                     VGEVLLKMGSVVLLKTDLDEIQLRQQIIYPFIPLLYEPIKIADYSTLNNSLKHTRTDI
                     VDMIADVSVDSVAYFIQSLQDFMTRYALANNRLEVANWIKAQFERFGITDSQLYPFNW
                     SGTTQYNVVATIPGTIYPDTYIIVGGHHDSITNDNPMTFAPGADDNASGTVAALEMAR
                     VMMANNYQPKCSIRFVTFAAEEFGLWGSKAYATMADQNNLDIRLMINHDMIANISPNP
                     WDSRVFLMPYDGYLDLTEYAAMLTSQYTTLLPVYGSMNLSASDSYSFWQHGFPVVYFF
                     EYNFSDVYHSNNDIVANLDPFYCTKVIRASTAVAVSFADMPSTPTNFTVQDEGTGSSI
                     HLSWNPVSDPTLSHYKISWGITQDT"
BASE COUNT          562 a          304 c          299 g          535 t
ORIGIN      
        1 gtatcctgag taatacccca ggaaatttta taatgcgaaa gagtaggatc ggaaactgga
       61 ttccaactaa gatgaatact gctaccggtg ccttcatcct gaacagtaaa atttgtaggc
      121 gtggagggca tatcagcaaa actgacagca acggcggtgg aagcacgaat tactttggta
      181 caataaaatg gatcaagatt agctacaata tcattattac tgtgatagac atccgaaaaa
      241 ttatactcaa aaaagtaaac gaccgggaag ccatgctgcc agaaggaata actatcactg
      301 gcgcttaaat tcatcgatcc ataaacaggc aatagagtgg tatattgtga agttaacata
      361 gctgcatatt cggtgagatc aagatatcca tcataaggca ttaaaaaaac tctcgaatcc
      421 cagggattgg gacttatatt agctatcata tcatgattaa tcattaaacg gatatccaga
      481 ttattttgat ctgccattgt agcatatgcc ttagaacccc aaagcccaaa ttcttctgct
      541 gcaaaggtaa caaaacggat ggaacatttg ggctgataat tatttgccat cattactctt
      601 gccatttcta aagcggcaac tgtgccactg gcattgtcat ctgctccggg agcaaaagtc
      661 attggattat cgttagttat ggaatcgtga tgtccaccta cgataatata agtatccgga
      721 taaatagttc ccggaatggt tgctactaca ttatattgag tggttccact ccaattaaaa
      781 ggatataatt gagaatcggt tatcccaaat cgttcaaatt gagccttaat ccaattagct
      841 acctctaacc gattatttgc caaagcataa cgagtcataa aatcttgtag ggattgaatg
      901 aaatacgcaa cactgtccac agaaacatca gcaatcatat ctacaatatc tgtcctggta
      961 tgtttgagtg aattatttag agttgaatag tctgctattt taatgggttc atataacaaa
     1021 ggaataaaag gatagattat ctgctgtcgc agctggattt catctaaatc tgttttcagt
     1081 aagacaaccg aacccatctt taataaaacc tctccaacta tgtttcgatc aaaatcggga
     1141 tgcctattac caattgtaac cagataaaat cttccgtctt gaggagttcc caaattaacc
     1201 gctttggggt aattacttac attggtgcct gcaataatat agaactcgtt atagtaatat
     1261 atttccagat cctgcgctga aagttcttta actttattgc gtaaggaatt ctcttctttg
     1321 gataaggaaa gttctgaagc aggaattccg ataataaatt taggtaaggc atttaacatt
     1381 cctgctgcta atataatcag cagtaggcta aacatttttc tcatatttat ctcctaagta
     1441 ttagaatgaa ataattgaag catatcatat tccatctttt ggttacttat atatgttatg
     1501 atgcaattta agattatgtt tcattactaa taaaatatag taaaatcagg gtattataaa
     1561 atcagtagac gctttaactt taatagcctc tcaggcttta tctatcttat tttttcatat
     1621 attttagttg aatgagcgag actaccatta tttttatatc ctttttagta atagtggcac
     1681 tgactattta tctagtaaca
//
LOCUS       DAORHB010000207         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_93949_length_1699_cov_4.565693, whole genome shotgun sequence.
ACCESSION   DAORHB010000207 DAORHB010000000
VERSION     DAORHB010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93949_length_1699_cov_4.565693"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..>1699
                     /locus_tag="PKM80_05685"
     CDS             120..>1699
                     /locus_tag="PKM80_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93437.1"
                     /translation="MLFCSIIYCQEAQNVTITDSLKTIVPELWTENWLYKRIYPLIFK
                     VETEREQEQEFYTAFIQYSGKTIANIFIVNLDVFDLLPDKPDKHIYNQILALGNSVHY
                     KTRDWIIEKMLFFAEGDSFDPQVMNRNLAYLKDLPYLREAELLISENENGTVDVFVLV
                     RDKFSLELSGKIISHNKYRIKINEQNILGLGLGLKHIWHIDPQALKSKSWESYYSNAN
                     IQGSFLRIDAFWKEFSGISTQDLYLSHPFLYPAIPHSGGIELSRNYTHPPVDSVTTEQ
                     ITLGSWYAHSFAYKDKEANAYKYVAGSLEKNWFPKRPQVNDNSGKPWHENIFALNSIA
                     FSKTGYTFLRDVSSYLENDVIPVGYLWENLLGYEFGEYRNRLFAGTHYSRADKFGSGS
                     YLYVSSALETYITTTGTEQSILAIEPLLISPLKQYGALRCRYFSNNNIIIGSKRYPKE
                     KMNLSSLSGYRGNWNLQGNKILRSCIENDMAIPYTIWGFKIGLFTFLDFAFATDDLSQ
                     MSTKNVLLTEGTGFKMHNP"
BASE COUNT          553 a          311 c          320 g          515 t
ORIGIN      
        1 ggcaagaaag gctattctgg aaaacagatg gaatgaattt ctggcagaaa tgctccccgt
       61 gctcgatacg atcgtttagt aaaaaagctg taatcaattt attctgcttt cttaccctaa
      121 tgctcttctg cagtataatc tactgccagg aagctcaaaa tgttacaatt actgattcct
      181 tgaaaacaat agtccccgag ctctggacag aaaactggct ttataaaagg atttatcctc
      241 ttatttttaa agtggaaaca gaacgcgaac aggaacaaga attttataca gcttttatac
      301 aatatagcgg taagacaatt gctaatattt tcatagttaa tcttgatgtg tttgatctgc
      361 ttcccgataa acctgataag cacatataca atcaaatctt ggctttggga aattccgttc
      421 attataaaac gcgggattgg ataatagaga agatgctttt ttttgctgaa ggcgatagct
      481 tcgacccaca agtaatgaac cgtaatcttg catatttgaa ggatttacca tatctgagag
      541 aagccgaact gctgatcagt gaaaatgaaa atgggacagt tgatgtcttt gtgttggtga
      601 gagataaatt ttccttggaa ctaagcggta aaattatctc ccataacaaa taccggatta
      661 agattaatga acaaaacatc cttggcttgg gtttagggct aaaacatatc tggcatatag
      721 atccacaagc tctgaaatcc aagagctggg aaagctatta tagcaatgcc aatattcaag
      781 gtagcttctt gcgtattgat gctttttgga aagaattttc aggaatatca acccaggact
      841 tgtatttaag ccatcctttt ttgtatcctg ctatccccca ttcaggagga attgaacttt
      901 ccagaaatta tacccatcca cccgtagatt ctgttactac agaacaaatt actcttggtt
      961 cttggtatgc acatagtttt gcctacaaag ataaagaggc aaatgcttac aaatatgtgg
     1021 ccggttcttt agaaaaaaac tggtttccca aaagaccaca agtaaatgat aatagcggta
     1081 aaccttggca cgaaaacatt tttgccctaa attcgatagc attcagtaaa acaggttaca
     1141 cttttttacg ggatgtaagc tcctatctgg agaatgatgt tatacctgtt ggttatttat
     1201 gggaaaatct cttgggttat gaatttggag aatatcgcaa tcgacttttt gctggcacac
     1261 attattctcg agcagataaa tttggtagcg gttcatatct ttatgtatcg agtgcactcg
     1321 aaacatatat tactacaaca ggtaccgaac aaagcatttt ggctattgaa ccattactta
     1381 tttcaccact taaacaatat ggagctctcc gctgtagata tttcagcaac aataatatta
     1441 taatcggcag taaaagatat cctaaagaaa aaatgaacct ttcgtcccta tcaggatata
     1501 ggggaaactg gaacttacaa gggaacaaaa tcttgcgctc ctgtatagaa aatgatatgg
     1561 ctattcccta tacaatttgg gggtttaaga taggattatt tacctttctg gattttgcct
     1621 ttgccacaga tgacctatcc caaatgagca caaaaaatgt gttgctgact gaaggcacag
     1681 gttttaaaat gcataaccc
//
LOCUS       DAORHB010000208         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_94096_length_1698_cov_2.850274, whole genome shotgun sequence.
ACCESSION   DAORHB010000208 DAORHB010000000
VERSION     DAORHB010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94096_length_1698_cov_2.850274"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKM80_05690"
     CDS             complement(<1..594)
                     /locus_tag="PKM80_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV93438.1"
                     /translation="MLNNKFSRYLIDNELFDEDTMEKAEKKMNEYGGTSSGALARVLE
                     IEFGAPHDKIYEALALHYAFPTFKHTIEEITEQQLEVCKNILSKLHRDTDEDFKKRLF
                     FNKILPFAIKRTPREVLQVLVSDPTNLLIQEIPVRTPYKRMEVYWAPVKLIEAIIEKV
                     APQQNEFLMMLEEAGQVLSNIEETEETVINEQELDEEI"
     gene            complement(608..>1698)
                     /locus_tag="PKM80_05695"
     CDS             complement(608..>1698)
                     /locus_tag="PKM80_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV93439.1"
                     /translation="MGYDKVQVNPVLFDLPSKPSVADIMMFIKLSSTMLRDKMNFGKI
                     LEMLAEEQPNRGFRDALLQIESQLKSGAEGREVFMRYSHIFGKFPAYMLGLATRSGNM
                     AEVFDATSKFIERDMEIKKNIKKATISPAFALLATIGATIYYIIDIFPATAKMFLKYD
                     LPLPPMTKATLEVSDWLGVYWWVILLIFLIPVIIIWRWWSTPKGRIWRDQHIVKLPLI
                     GNLLHKQSIEIYFRVFGTIYSGAGDNIETIKTAAEACRNAWMEDRIKKVTIPLMLKEG
                     EAFVPAMEQSGVFTRTALMRLKTGQETGTILQSAQQIATFYEAETTYKMNNLIEYIQS
                     IVGLVVAIAITFLTVVSAEIATISPPTY"
BASE COUNT          512 a          339 c          288 g          559 t
ORIGIN      
        1 tatttcttca tccagttcct gttcgtttat aactgtttcc tcggtttctt cgatatttga
       61 taaaacctga cccgcttctt ccagcatcat aaggaattca ttttgctggg gagcgacctt
      121 ttctattatg gcttctatca atttcactgg agcccaatat acttccatcc gtttataggg
      181 agtgcgaaca ggtatttctt gaatcagtaa attggtagga tcgcttacca atacctgtaa
      241 cacttcccga ggagtgcgtt ttattgcaaa aggaaggatt ttattaaaaa acaagcgttt
      301 tttgaaatct tcatctgtgt ctctatggag tttgcttaat atattcttac atacctccaa
      361 ttgctgttct gtaatttctt caattgtatg tttgaaggtt gggaaggcat aatgaagggc
      421 aagagcttca taaattttat cgtgaggagc tccaaattca atttccagaa cgcgcgctaa
      481 tgccccagat gaagttcctc catattcatt cattttcttt tcagcttttt ccatcgtatc
      541 ttcatcgaaa agttcattat cgattaaata tctggaaaat ttgttattga gcattgtaat
      601 attccttcta ataggtggga ggagaaattg tagcaatttc tgccgaaacg acagttaaaa
      661 aagtgattgc aatagctact actaaaccaa ctatactctg gatatattca attaagttat
      721 tcattttata agttgtttca gcttcataga aagtggctat ttgttgtgcc gattgtaaga
      781 tagttccagt ttcttggcca gtttttaaac gcataagagc cgtgcgggta aaaacacctg
      841 actgttccat agctggaaca aaggcttcac cttcttttaa catcaaaggg atagtaactt
      901 tcttaattct atcctccatc caagcatttc tacaggcttc agcagccgtt tttattgttt
      961 ctatattatc acctgcacca ctataaatag tgccaaaaac ccgaaaataa atctctatgg
     1021 attgtttatg taacaagttc cctattaaag gaagctttac aatatgttga tcgcgccata
     1081 ttctaccttt gggagtgctc caccaacgcc agataattat tactggaatt aaaaatatca
     1141 gaagaataac ccaccaataa actcccaacc aatcacttac ttccaaagta gctttagtca
     1201 taggaggcaa gggaaggtcg tattttaaaa acatcttcgc tgtagcggga aagatatcta
     1261 ttatataata gattgtagca ccaattgtag ccagcaaagc aaaagcaggt gatattgttg
     1321 ctttcttgat gtttttctta atctccatat cgcgctctat aaatttactg gttgcatcaa
     1381 aaacttctgc catattacca cttcgcgtag ctaaaccaag catataagca ggaaatttac
     1441 caaatatatg agaatagcgc ataaagacct cacgcccttc tgcacccgat tttagctgac
     1501 tttcaatctg caaaagagca tcacgaaaac cacgattggg ttgttcttca gctaacattt
     1561 ccaggatttt gccaaagttc atcttatctc taagcattgt agatgatagc ttaataaaca
     1621 tcattatatc tgcaacggag ggcttggatg gtaaatcaaa taacacagga ttaacttgaa
     1681 ctttatcata gcccattt
//
LOCUS       DAORHB010000209         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_100522_length_1632_cov_3.982879, whole genome shotgun
            sequence.
ACCESSION   DAORHB010000209 DAORHB010000000
VERSION     DAORHB010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100522_length_1632_cov_3.982879"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PKM80_05700"
     CDS             complement(<1..831)
                     /locus_tag="PKM80_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNV93440.1"
                     /translation="MMLFSYDKGQKVLSSKEIRQQFIDFFLSKDHIFIPSSSVIPEDD
                     PTLLFANAGMNQFKSIFLGQKEITIKRAVNSQKCIRAGGKHNDLETVGKDGYHHTFFE
                     MLGNWSFGDYYKKEAISWAWELLTEIWNIPKDKLYATVYLTDTEAFELWKQETDIDPT
                     HISYFEDKDNFWEMGETGPCGPCSEIHIDRGIEHCTMQNVQGHICQINGNCSRFIELW
                     NLVFIQYNREADNTLTPLKNKFVDTGAGFERLTQVLQDKNSNYETDLFMPIIHRIEEL
                     S"
     gene            complement(812..>1632)
                     /locus_tag="PKM80_05705"
     CDS             complement(812..>1632)
                     /locus_tag="PKM80_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV93441.1"
                     /translation="QINVTEEVENYPGFPEALSGYELTERMHRQAEHFGTEFKDEEVT
                     ALGMEGLCKIVETSDNKYRARAVIFCTGAYPRRLNVPGEEKFTGRGVSYCATCDGALY
                     RDKIVAVIGGGDSAIEEGIFLTHFAKKVVVIHRRDELRAQKIIQERAFKNPKMEFVWN
                     SVVQEIRGENKVQELEVYNRKTNTVSVIPVDGVFIYVGILPNNKLMESRIDLDSAGFA
                     LTDDFMHTNVPGVYAAGDIRKKVLRQVVTATSDGAIAGWSAEKWITENYDAIQL"
BASE COUNT          486 a          359 c          254 g          533 t
ORIGIN      
        1 agaaagctct tcaattctat ggattatagg cataaagaga tcagtttcat agttactatt
       61 tttatcctgc aaaacctgag ttaagcgttc aaatccagca cctgtatcta caaatttatt
      121 ttttaaggga gttaaagtgt tatctgcttc gcggttgtat tgaataaaaa ccagattcca
      181 taattcaata aaacgagagc aattgccatt tatttggcaa atatgcccct gaacattttg
      241 catcgtgcaa tgctcaattc cccggtcaat gtgaatttcc gaacagggac cgcaaggacc
      301 tgtctccccc atttcccaaa aattatcctt atcttcaaaa tagcttatat gagttgggtc
      361 aatatccgtt tcctgtttcc atagttcaaa agcttctgta tctgttaaat aaacagtagc
      421 atagagctta tctttgggaa tattccaaat ctcagttagc agctcccatg cccaagaaat
      481 agcttctttt ttatagtaat ccccaaaact ccagttacca agcatttcaa aaaaagtgtg
      541 atggtaacca tcttttccta ctgtttccag gtcgttatgt tttccaccag cacgaataca
      601 cttttggcta ttaacagccc ttttgatggt tatttccttc tgtcctaaga aaatactctt
      661 gaattgattc attcctgcat tggcaaaaag gagggttgga tcatcttccg gaattacaga
      721 tgatgaagga ataaaaatat gatctttact aaggaaaaaa tcaatgaatt gttggcgaat
      781 ttctttactg cttagcactt tctgtccctt atcataactg aatagcatca taattttcag
      841 ttatccattt ttcagcactc caaccagcta tggctccatc actggtcgct gttacaactt
      901 ggcgtagtac tttcttacga atatctccag cagcataaac tcccggaaca ttcgtatgca
      961 taaaatcatc agttagagca aaacctgcac tatcaagatc aatgcgagat tccattaact
     1021 tgttatttgg taaaatacct acgtagataa agaccccgtc aacaggaata acacttacag
     1081 tattggtttt ccgattgtat acttctaatt cttgcacttt attctctcct cgaatctctt
     1141 gaacaacgct attccagaca aactccatct tgggattttt aaatgctcgt tcttgaatga
     1201 ttttttgagc acgaagttca tctcgtctgt ggatcactac aacttttttg gcgaaatgag
     1261 tgaggaaaat accttcttca atagcagaat caccccctcc aataacagct acaattttat
     1321 ctctatataa agctccatcg caagtagcac aataagaaac tccacggcct gtaaattttt
     1381 cttcaccggg aacatttaga cgacgcggat aagctcctgt gcaaaatata acggctctgg
     1441 cacgatattt gttatctgaa gtttccacta ttttacaaag accttccatt cccagagctg
     1501 taacttcttc atctttaaat tctgttccaa aatgctctgc ttgacgatgc attctttctg
     1561 taagctcata accacttaga gcttctggaa atccaggata attttcaact tcttctgtaa
     1621 catttatctg ac
//
LOCUS       DAORHB010000210         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_103558_length_1603_cov_4.178941, whole genome shotgun
            sequence.
ACCESSION   DAORHB010000210 DAORHB010000000
VERSION     DAORHB010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103558_length_1603_cov_4.178941"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1205)
                     /locus_tag="PKM80_05710"
     CDS             complement(57..1205)
                     /locus_tag="PKM80_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNV93442.1"
                     /translation="MKKHLLFILILGCTLVSLAAQATKEEENDDKKVSAKFEININED
                     PINELSTYNKPYMGIRHKDITLPKAKELNYNGYAGILIVDVIPGSPAWQHHLQEEDIL
                     FCVDGKEITDSATFHKIIKNYSAGDKVILGIFRDGKTNEVEFTFGSRNKTELKIEEKT
                     CKNKSAGYRGITWLPMFVFLDMEDVNNLIASDTLSFGKLNEDGSLQQGIAGKFSIGKG
                     FFLGGQYSSYKDVKKHHNQTNPEYQLWMKYKNQMGGVTLDKRIPITKNFISSAGLMVG
                     GAKQIMEFQNSNSNFNWDSLPSTITHSNNTHFEITKGYLTVQPRFELMYRFLPWLALR
                     AEIGYAYSYNLTNYWKVTNIDGDPVEIKGSPDTNFQGLTLSIGPWLGF"
BASE COUNT          504 a          282 c          266 g          551 t
ORIGIN      
        1 tagtgcctct tcagacaatg attcgacacc cacaaacagg tgattctttt tagttattag
       61 aaccccagcc agggaccaat agaaagggtt aaaccttgaa aattagtatc tggagaacct
      121 ttaatttcta caggatcgcc atcaatatta gttaccttcc agtaattagt aagattataa
      181 ctataggcat aaccaatttc agcgcgaaga gccagccagg gcagaaaacg atacatcaat
      241 tcaaaacggg gttgaactgt aagatatcct ttagtaattt caaaatgagt attattggag
      301 tgggttatgg tagaaggtaa gctatcccaa ttgaagttag aattggaatt ctgaaattcc
      361 attatttgtt ttgctccgcc taccattaat ccagcggaag aaataaagtt cttagtgatg
      421 ggaatgcgtt tatctaaagt aactccaccc atttgattct tatatttcat ccagagttgg
      481 tattcaggat ttgtttgatt atgatgtttc tttacatctt tataggagct gtattgaccc
      541 cctaaaaaaa atcctttacc aatggagaat tttccggcaa taccttgttg taaagaacca
      601 tcctcattta gctttccaaa gcttaaagta tcggaagcaa tcaaattatt cacatcctcc
      661 atatccagaa atacaaacat aggtagccag gttattcctc tatagccagc ggacttattt
      721 ttgcaggttt tttcctcaat tttcagctca gttttatttc gagagccaaa agtgaattca
      781 acctcatttg tttttccatc tctgaatata cctaaaatca ccttgtctcc agcgctgtaa
      841 ttcttgatta ttttatggaa agtggcagaa tctgttatct cttttccatc aacacaaaat
      901 agaatatctt cttcctgcag atgatgttgc caggcaggag aaccaggaat tacatcgaca
      961 atcaaaatgc ctgcatatcc attgtagttt aattcttttg cttttggtaa tgtaatatct
     1021 ttatgccgaa ttcccatata gggtttatta taggttgata attcattaat gggatcttca
     1081 ttgatgttaa tttcaaattt agctgaaacc tttttatcat cattttcttc ctctttagtc
     1141 gcttgagctg ccaaggaaac gagagtgcat cctaaaatta ggataaacaa aagatgtttt
     1201 ttcattattc ttatcctctt gtgcattttt atatattaac tttataatac cagcttatgt
     1261 gataatctga gctctatcgt ttgttttcaa ttatgttcca caaaaattat caataattat
     1321 tagataacat tctgtcaaga ttttgttttt ccatattaac ttaccttcac atcctgtgtc
     1381 ttaaaatttg ccttttctgg ttactgcgtt attctatata taggttataa cttcagctgg
     1441 ggtaattacg acaaaataaa aattagcatt ttcgtattta cctgatagta aataagagga
     1501 ggttataaaa aaggaaagta attatgcaaa aagaagattt caacggtttg gaagaatatg
     1561 aactggaacc tgaggtctgg gaagaggtcg aaaaaactga aca
//
LOCUS       DAORHB010000211         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_1215
            NODE_109016_length_1555_cov_4.228667, whole genome shotgun
            sequence.
ACCESSION   DAORHB010000211 DAORHB010000000
VERSION     DAORHB010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854420
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:00:45
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1,144
            CDSs (total)                 :: 1,127
            Genes (coding)               :: 1,127
            CDSs (with protein)          :: 1,127
            Genes (RNA)                  :: 17
            tRNAs                        :: 15
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109016_length_1555_cov_4.228667"
                     /isolate="AMR_MDS_1215"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..102
                     /locus_tag="PKM80_05715"
     tRNA            27..102
                     /locus_tag="PKM80_05715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60..62,aa:Gly,seq:ccc)
     gene            276..1472
                     /locus_tag="PKM80_05720"
     CDS             276..1472
                     /locus_tag="PKM80_05720"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNV93443.1"
                     /translation="MKQVVVVCAKRSPIGNFGGVFKDISAVQLGVNTLKAMFTETGIK
                     PDMIDEVIIGNVCGAGLGQNVSRQIAIHSGIPETVPAYTVNKVCGSGMKAVSLATLMI
                     STGEADIIVAGGAENMSQIPYALKDARWGARMGSKDMVDLMIRDGLSDIFNDCHMGVT
                     AENLAEKYNISRAEQDAFAASSQNKTEAAQKTDRFKDEIVPVIIPQRKGDPIIVDKDE
                     MPRAGVTVESISKLRPAFKNDGTVTAANASGINDSAAFLILMSADKAKELNLTPLATF
                     VNSASAGVDPSIMGYGPVPAIKKVLQKANWKLSDIELAELNEAFAAQSLAVFKGLELE
                     GIGKLNPEITNVNGGAIALGHPIGASGARIIVTLLHEMKRRNVHKGLSALCIGGGMGI
                     ATLWER"
BASE COUNT          496 a          249 c          352 g          458 t
ORIGIN      
        1 tccaattatt ggaattcatc taatacgcgg gcatagctca gttggtagag cattagcttc
       61 ccaagctgag ggtcgcgggt tcgagtcccg ttgcccgctc catttttttc agttatgatt
      121 tgatgatttt tagatttttt aactcactct cgaggtaagc atatatatta aatctctatt
      181 aacctgttcc cttttttcaa gattctcatt gacgaaaact cgctttcttt ttgtgttggt
      241 gcaaatggat aaataatact gataaggaga aacaaatgaa acaggtcgtt gtagtttgtg
      301 caaaacgcag tcccataggt aattttggag gtgtctttaa agatatctcc gcagtgcaat
      361 taggagtaaa cacattaaaa gcaatgttta ctgaaacagg tatcaaacct gatatgattg
      421 atgaagttat tataggaaac gtttgtggtg caggtttagg acaaaatgtt tcgcgtcaga
      481 tagctatcca cagtggcatt ccagaaaccg ttccggctta tacagttaac aaagtatgtg
      541 gttcaggaat gaaagcagtt tcattagcta ctttgatgat tagtactgga gaggcagata
      601 tcattgttgc tggtggtgct gaaaatatga gccagattcc ttatgctttg aaagatgctc
      661 gttggggagc cagaatgggt agtaaagata tggtagattt aatgatccgg gatggtctta
      721 gtgatatatt taacgattgt catatgggtg tcacagcaga aaacttagct gaaaaatata
      781 atatttccag agcagaacag gatgcttttg ctgccagtag tcagaataaa acagaggcag
      841 ctcagaaaac agatagattt aaggatgaaa ttgttccggt tatcattccc caaagaaagg
      901 gagaccctat tatagttgat aaagatgaaa tgcccagagc tggtgtaact gttgaatcca
      961 tatctaagct gcgtcctgct tttaaaaatg atggaaccgt tacagccgca aatgcaagtg
     1021 gaataaatga cagcgctgct tttttaattt tgatgagtgc tgataaggca aaagaactga
     1081 atttaacacc tctagccaca tttgttaaca gtgcttcggc aggtgttgat ccttctatta
     1141 tgggttatgg tcctgttcca gcaattaaaa aagtgttaca aaaagcaaat tggaaattaa
     1201 gtgatataga gcttgcagaa ctgaatgaag cctttgctgc tcagtcactt gctgttttca
     1261 aaggattgga actggaagga attgggaaac ttaatcctga aatcactaat gttaacggtg
     1321 gagcaatagc tttaggccat ccgattggcg caagtggagc ccgaataatt gtaactttgc
     1381 tgcatgaaat gaaaagaaga aatgttcata aaggactttc tgctttgtgt atcggtggcg
     1441 gaatggggat agctacactc tgggaaagat aaaaaaaaga attttgagaa cactaataaa
     1501 aaagaacacc gaatcttcta acgacaagga catattgtaa aggtacgata taaag
//
