LOCUS       DAORIN010000001        23354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_348_length_23354_cov_5.794197, whole genome shotgun sequence.
ACCESSION   DAORIN010000001 DAORIN010000000
VERSION     DAORIN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 23354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23354
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_348_length_23354_cov_5.794197"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PK109_00005"
     CDS             complement(<1..734)
                     /locus_tag="PK109_00005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017725999.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRH23966.1"
                     /translation="MNTGTITEVIGPVVDVHFEENRPAIGDALVIEKAGEHGRITLEV
                     ASHLGLDRVRAISMNETAGLSRGEKAEATGAPISVPVGEVALGRLFNVLGEAIDGKAP
                     IAKDAKRLPIHRDPPGFDEQTTKAEVFETGIKAIDLMIPFLKGGKVGLFGGAGVGKTV
                     IIQELIRNIATEHSGYSVFAGVGERVREGNDLYHEMAESGVLDKTALVFGQMNEVPGA
                     RARVALTGLTQAEYFRDEGGKDVLFF"
     gene            complement(757..1665)
                     /gene="atpG"
                     /locus_tag="PK109_00010"
     CDS             complement(757..1665)
                     /gene="atpG"
                     /locus_tag="PK109_00010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRH23967.1"
                     /translation="MASLKDIKAKIGATKRMNTVTRAMEAVSAVKMRKTQASALAGRP
                     YARAGLAILARLSGSQDALRHPLAQVRDVKKLGLVVMTSDKGLAGALNSAVIKRAAEA
                     VEAFDKKDVTVFAYGKKGEEYFARRGYTVKKAYQNKRDDVPISVMEELSRELSAGFLA
                     EEFDQVFVAYSNFKSTFEQVATGRVLLPLTLEAVTELVADIRPAKGKWSEEVAIEAPA
                     SYEVESTEDILGVLIPRLVAVSLYHMLLESKASEHSARMVAMKSASDKSKEVARDLTR
                     LYNKIRQAAITREVSEIVGGREALAT"
     gene            complement(1684..3234)
                     /gene="atpA"
                     /locus_tag="PK109_00015"
     CDS             complement(1684..3234)
                     /gene="atpA"
                     /locus_tag="PK109_00015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRH23968.1"
                     /translation="MESIIKRIEKEIAGFTPTGGSEETGIVRATGDGIAEIDGLDNAV
                     MTEMVLFDATLDRTLEEALKDANPVYGLILNLEEDGVRAAILGDATKVYEGMLVRRLG
                     RLLSIPVSDDMIGRVVNPLGEPVDGKGPIPNTKFRPIEREAYGVIDRAPVNVPLHTGI
                     KAIDAMVPIGRGQRELIIGDRGVGKTTIAIDAILNQKNEPKDSRPICIYVAIGQKESK
                     TAKIVAQLAEAGALEYTIVVTAPASSPAAYQFLAPFAGAAIGEHFMEQGKDGLVIYDD
                     LSKQAVAYRQLSLLLRRPPGREAYPGDIFYLHSRLLERAAKLNKELGGGSLTALPIIE
                     TQEGDVSAYIPTNVISITDGQIFLLTDLFNKGMRPAIDVGISVSRVGSSAQTKAMKKV
                     SGKIKLELAQFRELEAFMQFSSDLDEGTKKQIDSGRRMTEVLKQGNGAPLAFEMQAAV
                     IFAAINGYFDTFSPETTSAMEKKLQDYLVREGGDVLAAIRSKKEIGEDTEKLLRSLLE
                     KFVERSGK"
     gene            complement(3256..3624)
                     /locus_tag="PK109_00020"
     CDS             complement(3256..3624)
                     /locus_tag="PK109_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23969.1"
                     /translation="MKAATYAKAIDRLVEAGTDQAKLAENLLKNLSATGRMKLLPGIL
                     GELKAYEARRQAVAPRVEVAHEKDASAALAAAKAEGITAEKAHVNHSLIRGWRARSRG
                     TLVDRSSKRALIDLYRRIAN"
     gene            complement(3627..4070)
                     /locus_tag="PK109_00025"
     CDS             complement(3627..4070)
                     /locus_tag="PK109_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23970.1"
                     /translation="MDALLDAFGIDWKLLIAQAVNFGILIVALYFLLYKPVLKVLEER
                     RVMVAKGVEDAERATQKLAGADAAAAATVEKADKEAAEVLASARDAAATERTRLVKEA
                     EARAAAISADAEARSKEAAAKALRDSEKEIARLAILAAAKVAGEK"
     gene            complement(4080..4307)
                     /locus_tag="PK109_00030"
     CDS             complement(4080..4307)
                     /locus_tag="PK109_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRH23971.1"
                     /translation="MELESARVIGMAIAAGLGVFGPGIGIGLIGSGAMQAIGRNPEAS
                     GKVVSNMILAIVFTEALGILALVVSFIIKFV"
     gene            complement(4371..5177)
                     /gene="atpB"
                     /locus_tag="PK109_00035"
     CDS             complement(4371..5177)
                     /gene="atpB"
                     /locus_tag="PK109_00035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRH23972.1"
                     /translation="MATEAAHLATTTAAHAVEHVSPIHVVLKAEELFSVWGFPITNSL
                     LLTWITMAVLIVFAFFFGRSLKAVPGRLQAGIEWAFEGVINFMEETLESEKLARRFFP
                     LIATIFLFVFVANELAFIPGVGSILFGHEGEMAPFLRAPAADLNFTLALAIISVLTIE
                     ITGIVTLGLLKYGGKFVNFKSPVDFAVGLIELISNLGRLISFSFRLFGNVFAGEVMIL
                     VAGYFLAYLLPVPLLAFEVFVALIQAVVFSMLTLFFIKLAIMEPHGEEAH"
     gene            complement(5298..5723)
                     /locus_tag="PK109_00040"
     CDS             complement(5298..5723)
                     /locus_tag="PK109_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23973.1"
                     /translation="MAPRKKTAKKVVRKAPAKRKTSRQYEGEIADLGERLKISQHRYT
                     TLASAVESERALSKTVVTAYEEQKQVNAALSGRYNDLVEMHNNVLDENDKFRLGTIRL
                     EGKIEGMMFAYQHALETVLDNLSISMLVKGELTATKPKD"
     gene            complement(5794..5866)
                     /locus_tag="PK109_00045"
     tRNA            complement(5794..5866)
                     /locus_tag="PK109_00045"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5831..5833),aa:Ala,seq:ggc)
     gene            complement(5879..6367)
                     /locus_tag="PK109_00050"
     CDS             complement(5879..6367)
                     /locus_tag="PK109_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23974.1"
                     /translation="MTKTPQIIGPVERATTWVGSVASLIVHTVAFAAAFAVGLFGLAS
                     WELVLLILTTIVSLEAIYLAIFIQMTVNRNTAQLREVEEDIDEIQEDVEELGEDMDEI
                     QEDIEEIQEDVDEIQEDVEEMSEEEKEEEKRIASEGRSLDELTGDIRRILTELEALQK
                     KQ"
     gene            complement(6523..7107)
                     /locus_tag="PK109_00055"
     CDS             complement(6523..7107)
                     /locus_tag="PK109_00055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRH23975.1"
                     /translation="MEIIRPESRQPLPPHAKRVFKGDIFDVYQWEQELFDGSTKTFDK
                     VKRDDTIVVIPTLPGKKLLFLKDEQPARSAIHTFPAGRMDKEGEGPLEAAQRELKEET
                     GYESDEWTLWKAYQPVTKIDWAVYVFIARNCVRTTEPALDPGERITVKEVTLDDVIAL
                     VDDPDFLSEDMKAELIAAKYDKASREVLETTIFG"
     gene            7156..7229
                     /locus_tag="PK109_00060"
     tRNA            7156..7229
                     /locus_tag="PK109_00060"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7190..7192,aa:Val,seq:cac)
     gene            7249..7319
                     /locus_tag="PK109_00065"
     tRNA            7249..7319
                     /locus_tag="PK109_00065"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7281..7283,aa:Pro,seq:cgg)
     gene            7462..7719
                     /locus_tag="PK109_00070"
     CDS             7462..7719
                     /locus_tag="PK109_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HRH23976.1"
                     /translation="MPRALNPALTKPMNLSPELEAVVGKGPMPRSEVVKQIWVYIKKH
                     DLQNPANKRNILADDKLKAVFGGKGEVTMFEMTKLVSAHLK"
     gene            complement(7774..7959)
                     /locus_tag="PK109_00075"
     CDS             complement(7774..7959)
                     /locus_tag="PK109_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23977.1"
                     /translation="MAYTDQNKGDIKELVRKAYKILDEALYIFDEKTEGNQDRVARTL
                     ADAAKYLDRARNWIRER"
     gene            complement(7978..8667)
                     /gene="lexA"
                     /locus_tag="PK109_00080"
     CDS             complement(7978..8667)
                     /gene="lexA"
                     /locus_tag="PK109_00080"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRH23978.1"
                     /translation="MSRNDSLTEVQEATLAFIKRYIAQHGQSPTLTELQTLLKLSSLR
                     SVTQRIEALERKGFIARDRFQHRSIRILKTISSQYLPSDFIAVPVIASAGCDAMEVYA
                     QDEFGEFIAVDKKLLGKATSIAAIKAVGDSMEDAGIKNGDYVIVEVTDRVADGDRVVA
                     IIGNMAVIKRLEHSNGFAVLHPENAAANYAPIVVQEEDSKVFGKVLSIIPGTDWQDDV
                     KIEYYDGFGPR"
     gene            complement(8726..9133)
                     /locus_tag="PK109_00085"
     CDS             complement(8726..9133)
                     /locus_tag="PK109_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23979.1"
                     /translation="MSPEAPAPSHERSYNYEPKTIAQMNDIVRGVLMERFPDRAIELT
                     TTVSHAASKFKPHTRREAIVQRSNLYDGNAQAEILSMVLWSYILKHESKAHISSPTVF
                     LKRLEEAEGEKREVMQSLVELHDTLYKETLKRL"
     gene            complement(9339..9557)
                     /locus_tag="PK109_00090"
     CDS             complement(9339..9557)
                     /locus_tag="PK109_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23980.1"
                     /translation="MDWLTPLYKISLSALKIVGLFYVLTLIFSGAYFFATNILGIDEN
                     SAVTDMWFLGILGCIAIGVLQYEDKYGK"
     gene            complement(9600..10433)
                     /locus_tag="PK109_00095"
     CDS             complement(9600..10433)
                     /locus_tag="PK109_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23981.1"
                     /translation="METIYLAFTTLLTRLYIFVMEVLEVIHEWQDFYGALLAAGVAII
                     LFILTEWAKERKERQDQTFRLHRSIGAALSNLTEIDSTLTHFSNNQLARIISQTEHDI
                     SNGQPTIRYAFVPLLHVAEIPEELLKQSSGSGYVDALTIDLVNKSKDLRKIIDDINRQ
                     FEASILLNNHIAMNRLNPDPRVQSRAFKVNMEEFRSKMIERDLRNNIQIYASQLIKAQ
                     TAVSKLQDWGLKKWKKSFNHFNGRDMYERMDNYFTNDVSLRKVAYQEVFQTPICVTIL
                     N"
     gene            complement(10525..10597)
                     /locus_tag="PK109_00100"
     tRNA            complement(10525..10597)
                     /locus_tag="PK109_00100"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10562..10564),aa:Ala,seq:tgc)
     gene            complement(10902..11234)
                     /locus_tag="PK109_00105"
     CDS             complement(10902..11234)
                     /locus_tag="PK109_00105"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRH23982.1"
                     /translation="MTSLAGKRCLPCEGIIDKLTPEEAKKLHADVPEWTLSEDATTLT
                     RTFPFKDFAKALAFANKVGEVAEAEWHHPDMRVGWGKVEVQFTTHSIRGLAENDFIMA
                     AKVDALSA"
     gene            complement(11227..11382)
                     /locus_tag="PK109_00110"
     CDS             complement(11227..11382)
                     /locus_tag="PK109_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23983.1"
                     /translation="MNNERPFKRKMAHGPDDGLLPEYLIDGEPDLDDLEKETERKNAL
                     KKEEGYD"
     gene            complement(11388..12356)
                     /locus_tag="PK109_00115"
     CDS             complement(11388..12356)
                     /locus_tag="PK109_00115"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRH23984.1"
                     /translation="MRGTIVGVLRGGPSKEHEVSLKTGAAILKNLDIGRYTVRDIYID
                     KQGNWHVHGRPTTPPEALRSVDVVIVGLHGEYGEDGEVQKILERHGIPYTGSNSFASY
                     LAMHKVMAKERARELGVLTPEYRFIQNAEQAEEILADVIRSFHQPVVVKPIRWGSSVG
                     ISMVSGYAPLHAAVMQLLSAGAGGVLIEEVIRGREATQGVVQNLRGEKLYALPSIEIV
                     PPENDFFSYDAKYSGKSREICPAPFSKKEQEELARIARVMHEGLGLRHYSRSDFIISP
                     KGIYYLETNTLPGMTSESLLPKSLAAVGVSFPDFLSHLVDLSVAGI"
     gene            complement(12371..13627)
                     /locus_tag="PK109_00120"
     CDS             complement(12371..13627)
                     /locus_tag="PK109_00120"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRH23985.1"
                     /translation="MERAYLTKLDLPDVPGVYLFTQGKGKSKKILYVGKATSLRDRVR
                     SYFDVDLIATRGPRIVDMVTLSDGLMHETTPTVLEALVREAALIREHKPKANAIGKDD
                     KSFLYAVITDEEIPRVLAIRGTDLDFKKKEITIGSIPLRAMHGPFPSGYQLREALRLI
                     RKIFPFYDTPRPVGEGSRFTQGKVEFNKQIGHYPRDLDRTGYLRSIRNVSMFLSGRVK
                     TLRAELQKQMKLAAKAERFEDAALARQQLFALDHVQDISLIREDRKEEQNEPRVEAYD
                     TAHLSGTNAIGVMVVIDNGLPDKKEYRSFKIRGAGGKSLNDDIASLKELLNRRFGHPE
                     WQYPKAIVVDGGKTHKKAAEETLKELGIGIPVIAVVKDERHRPREVIGSLRAGIPDAT
                     VVLANSEAHRFSITLHRKARRRSLKA"
     gene            14112..14378
                     /locus_tag="PK109_00125"
     CDS             14112..14378
                     /locus_tag="PK109_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23986.1"
                     /translation="MCGLLREPEAISGNDMSDTVLKIEKVAALKQCILAAAALALSVW
                     GCLLGMSYNHEDAGLVLLVSSLYHVVKLYCEVQNLYALKQVCHA"
     gene            complement(14431..17070)
                     /gene="uvrA"
                     /locus_tag="PK109_00130"
     CDS             complement(14431..17070)
                     /gene="uvrA"
                     /locus_tag="PK109_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRH23987.1"
                     /translation="MPKSKKTDAHTQYVGDDWIEVKGARTHNLKNINVDLPRGTMTVI
                     TGLSGSGKSSLAFDTIFAEGQRRYVESLSAYARQFLNQMQKPDVDEIRGLSPAISIDQ
                     KSRSNNPRSTVATVTEIYDYLRVLYARAGQPYCIHHDVPIQKLSKEEMIRLIMDRIKK
                     RSGSTEHSMAVKGVVVDKTMVTIYAPVVVGRKGEYYQLLYDLLGKGYEKAVIDGKVHG
                     LREKVTLDRNKRHDIDAVVDGIFVSEFIRTPDAARERLSEALELALKESDGLVKIGHA
                     DGTTETLSARFICPEDGASFPEVEPRLFSFNSPYGACPNCNGLGTESLFSETICPVCE
                     GKRLRPEALRVYLKGKSDKTLGKNVVDITDMSIREAKNFMDDLHLSDMKAEIAFPIIR
                     EITSRLDFMLNVGLDYLTLNRSAATLSGGEAQRIRLASQLGTGLVGALYVLDEPTIGL
                     HQRDNDRLIKTLLTLKELGNTILVVEHDEDTILSSDYLVDIGPGAGVHGGNVVVAGWL
                     DELLRAPKNESGSLTLDYLRGDMEVAVPEERRTSEKGSIKIKGATLHNLKNQNVEVPL
                     GKLVGITGVSGSGKSTFLYDILHKNVAARFNRRETKPEHVASITGTEYVGRVVLIDQS
                     PIGRTPRSNPATYTGAFTHIRDLFSATSEARARGWKPGRFSFNVAGGRCEACQGNGVI
                     AVEMHFLPTVYVQCDVCDGKRFMKETLEVTYRGKNIYDVLQMTVEEAEVFFKDIPAIS
                     ERLASLNSTGLEYLTLGQSATTLSGGEAQRVKISAELYRPTTMKTLYLLDEPTVGLHY
                     DDVRKLTEILQELVRRGNTVVVIEHNLDVIKSCDYLIDFGPEGGTGGGKVIAKGTPEE
                     VADTEGSHTGEYLSKLLRKKKKK"
     gene            complement(17153..17545)
                     /locus_tag="PK109_00135"
     CDS             complement(17153..17545)
                     /locus_tag="PK109_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRH23988.1"
                     /translation="MPETRFILVVEDDPILKNLLGHTFAGKYQTLYASDGNEALALFE
                     ANKPALVLLDLMLPTMDGFAVLETIKKRTDALKDVPVIVVSNLGQDKDKERALALGAT
                     EYLVKAEVSVEEIVGRIEKVLGTITPNV"
     gene            complement(17565..18659)
                     /locus_tag="PK109_00140"
     CDS             complement(17565..18659)
                     /locus_tag="PK109_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRH23989.1"
                     /translation="MSFRSLLGITLFIACAVAATVILLSILYLRGQISALLPYDLALE
                     VDASLMNLFMMQAIALFVLALGLSVAIFFVVGSFVSKPLRELTRAMDGYATSGARTTI
                     DGIDEAPKEIRSLAVSFDGLIDRVEESHKHDTDISRVKSDFISTAAHQFRTPLTGIRW
                     ALEALQKENLTESQKALVESAVGKSKDLVAIVGTLLDISAIESGKYKYTFAPTDMNAL
                     VTEIVASFEPLAQKTQISLFYTHEASEALPAAKADRERIKWVLNNLIENAILYTPAGG
                     TVRVSSEAGGGRVYVRVKDTGIGIPGEDRANIFERFYRAQNAITVKNQGNGLGLYIAR
                     TIATDHGGDLNFAANTEGPGTTFTLSLPLA"
     gene            complement(18721..19038)
                     /locus_tag="PK109_00145"
     CDS             complement(18721..19038)
                     /locus_tag="PK109_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23990.1"
                     /translation="MPRIQVIALFPSETIPEYVRQLLVGRIFETYSSFVSVPALKVVD
                     SVGNQIPDDGWRGYVIDKEHFLSVLDEECPDAAHWYRTVSILKEPNVKVYLLETRYCR
                     IVR"
     gene            19119..19310
                     /locus_tag="PK109_00150"
     CDS             19119..19310
                     /locus_tag="PK109_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23991.1"
                     /translation="MKNPYFWWVVLVTLGFAVVYSLPIGGTGGIVILHLLWKMVAPFI
                     MVAGVLTLLYMIFTGSPKK"
     gene            complement(19311..21374)
                     /gene="uvrB"
                     /locus_tag="PK109_00155"
     CDS             complement(19311..21374)
                     /gene="uvrB"
                     /locus_tag="PK109_00155"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRH23992.1"
                     /translation="MSAGFKLHLPFEPAGDQPEAIKALSKGVADGLTHQTLLGATGTG
                     KTFTIANVINNAQKTTLVLAHNKTLAAQLAQEYREFFPENAVHYFVSYYDYYQPEAYI
                     SHSDTYIEKEAQINAEIDRLRHAATQALLTRKDVIIVASVSAIYGLGSPAQYEKFHVK
                     LAKGAEENRAALIRKLIGIYFERTPADLEPGKIRAVGNTVEFMPVNERVIYRVTFDGG
                     VIGTIQQLDPVSRALVAEPDTTFIFPAKHFVTSDDERDRALEDIRLELDETLAKFKRE
                     EKLLEAERIKRRTMHDLALIKEIGYTSGIENYSRHFDGRKAGEPPHTLLSYFPHKKDG
                     SADFLTVIDESHVTVPQIGGMFAGDRSRKDNLVEFGFRLPSARDNRPLTFEEFEERVG
                     QTIYTSATPGPYEREHSVPPKGQIAEQIIRPTGLIDPELVIRPINEKGDYKGQVADFI
                     AEAEKTINGGGRVIATTLTKQMAEDLAEFLKERKIKAEYLHSDIKTIERIDILTNFRK
                     GNFDILVGVNLLREGLDLPEVELVGILDADKEGFLRSETSLIQTIGRAARNVLGRVIL
                     YADVMTGSLTRAIGETERRRKIQLAYNEKHGITPQTIKKEIRDIAESMRSEHDKTVGD
                     MLSLDKRLYEKDPERFVKEMRQRMGEAVEALDFETAALIRDELYELEGNGPKKKVERK
                     RKRFS"
     gene            complement(21515..21856)
                     /locus_tag="PK109_00160"
     CDS             complement(21515..21856)
                     /locus_tag="PK109_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23993.1"
                     /translation="MKTVKARMLNLTAIAAVLSVPEIANQDADLGNDLATFYNDTKRN
                     HPFDEMTAITRDERRGGVFVTTVSVMLGDPAFKDQVERLAKNHPAAIDRLLPFADQLQ
                     RARRSTAPASS"
     gene            complement(22025..22474)
                     /locus_tag="PK109_00165"
     CDS             complement(22025..22474)
                     /locus_tag="PK109_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23994.1"
                     /translation="MSNSASAVARQDQEETVDATQAILKQVEALLAKPIALSTKFLEV
                     IDFFTSEGVSEIDEDLGETLKQMESGDWGETFAHLVRSNKEREDHYTDVLTVSKAVHA
                     IATDYVALLEFVIESEEGIEKVWAAMMRAKDADWKPEPEGDGATGGE"
     gene            complement(22557..>23354)
                     /locus_tag="PK109_00170"
     CDS             complement(22557..>23354)
                     /locus_tag="PK109_00170"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRH23995.1"
                     /translation="IDDEGVTFTSHLDGTLHRFTPERSIEIQHALGADMFFAFDECTA
                     PTESYEYQKEAMYRTHRWAERSLKAHRQNLAANKTQGIFGIVQGGRHLDLRKESANTI
                     ASMDFDGFGIGGSFSKEDMDGALQAAIPELPEGKPRHFLGIGEPTDVLTGIAEGMDLF
                     DCVAATRIGRHGSIYTRRGIKHLRNGEFKNDFSPLDPETIVSGTEGFTKAYVSHLVRS
                     KEMLGSVIASMHNLGFILKLVDGARDALHNGTFEQYRADFLRDYKGT"
BASE COUNT         5586 a         6119 c         5732 g         5917 t
ORIGIN      
        1 atgaagaaga ggacgtcctt tcctccctcg tcgcggaagt attctgcctg cgtaagaccg
       61 gtgagcgcaa cgcgagcgcg tgctcctggc acttcgttca tctgaccgaa cacaagcgcg
      121 gtcttatcca agacgcccga ctctgccatt tcatgataga ggtcgtttcc ttcacgcacg
      181 cgctctccca caccagcgaa cactgagtag ccagagtgct ccgttgcaat gttgcggatg
      241 agctcctgga tgataaccgt cttacccacg ccggcgccgc cgaagagacc tacctttcct
      301 cccttcaaga acggaatcat gaggtcaatt gccttgatac ccgtctcaaa gacttcagct
      361 ttggtggtct gctcatcaaa tcccggagga tcgcgatgta ttggaagacg ctttgcgtct
      421 ttggcgatag gcgctttgcc atcgatagct tccccaagca cgttgaagag gcgacccagc
      481 gccacttctc ccacaggaac ggaaatcgga gcaccggtag cttctgcctt ctctccacga
      541 gaaagtcctg ccgtctcgtt catcgagata gcgcgcacgc gatcaagtcc caagtgagac
      601 gcgacttcaa gcgtgatgcg gccatgctca cctgctttct cgatgacgag tgcgtcacca
      661 atggctgggc gattctcttc aaaatgtacg tcgacaactg ggccgatgac ttccgtgata
      721 gttcctgtgt tcatatgttt gtaagttagg gtaatgctac gttgctaatg cctcccgacc
      781 gcccactatt tccgagactt ctctcgtaat agcggcctga cgaatcttgt tatacagacg
      841 cgtgaggtcg cgcgccactt cttttgattt atccgacgcg ctcttcatag cgaccatacg
      901 cgcggaatgt tccgacgcct ttgattcaag caacatgtgg tagagcgaga cggccaccaa
      961 gcgtgggata agaacgccaa gaatgtcttc ggtagactcc acttcatatg acgctggagc
     1021 ttcaatagca acctcctcac tccactttcc ttttgccgga cgaatgtcag caacgagttc
     1081 cgtcacagcc tcaagcgtga gtggaagcag cacgcgtccc gtggctactt gctcgaacgt
     1141 cgacttaaag ttgctgtagg cgacgaagac ctgatcaaac tcttctgcaa ggaagccagc
     1201 agacaattct cgtgagagct cttccataac agaaatcggc acatcatcgc gcttgttctg
     1261 atacgccttc ttaacggtgt agccacggcg tgcgaagtat tcttcacctt tctttccgta
     1321 cgcaaacacg gttacatcct ttttgtcgaa tgcttccacc gcttctgcag cgcgcttaat
     1381 gactgcgcta ttaagagctc cggcaagtcc tttatcagag gtcatcacca cgagtccaag
     1441 cttctttacg tcccgcacct gcgcgagcgg atgacgaagt gcatcctgag atcctgagag
     1501 gcgcgcaaga atagcaagac cggcacgtgc gtacgggcgt cctgcaagcg cagacgcctg
     1561 cgtcttacgc atcttcactg cagacaccgc ttccatagcg cgcgtgacgg tattcatgcg
     1621 cttggttgcg ccgattttcg ccttgatgtc ttttagtgaa gccatagcag aggaacgtac
     1681 gtattacttc ccggagcgtt caacaaactt ctcaagaaga gagcggagaa gtttctcggt
     1741 atcttcaccg atttctttct tggaacggat agcggcaagc acatccccac cttcgcggac
     1801 gaggtagtcc tggagtttct tttccatcgc gctcgtcgtt tctggtgaga aggtatcgaa
     1861 gtacccgtta atagcagcga agatgacagc agcctgcatt tcaaacgcaa gcggtgcacc
     1921 attaccctgc ttcaatactt ccgtcatacg gcgacctgag tcaatttgct tcttggtgcc
     1981 ctcatcgagg tcagaagaga actgcataaa ggcttcaagc tcacggaact gagcgagctc
     2041 aagcttaatc ttgccggaca ccttcttcat agctttggtc tgggcgcttg aacccacacg
     2101 cgacacggag atacccacgt caattgctgg gcgcataccc ttgttgaaga ggtcggtgag
     2161 aaggaagatc tgaccgtcgg taatagaaat cacgttcgta ggaatgtatg ccgagacgtc
     2221 gccttcctgc gtttcaataa taggaagcgc ggtcaatgaa cctcctccca gttccttgtt
     2281 caactttgcc gcacgctcaa gaaggcgtga gtgcaagtag aagatgtctc ctgggtatgc
     2341 ttcgcgtcct ggtgggcggc gaagaaggag ggagagctgg cgatacgcaa ctgcctgctt
     2401 tgaaaggtcg tcgtaaataa cgagaccatc ctttccctgc tccatgaagt gctcaccaat
     2461 agcagctcct gcaaatggtg cgaggaactg gtacgccgca gggcttgagg caggcgccgt
     2521 caccacgatg gtgtactcaa gcgcacccgc ttcagccaac tgcgccacaa tctttgcggt
     2581 ctttgattcc ttctgaccaa tagcgacata gatgcagata ggacgagagt cctttggctc
     2641 gttcttctgg ttcaaaatag cgtcgatagc aatagtcgtc tttccaacgc cgcggtcacc
     2701 gatgatgagt tcgcgctggc cacgacctat tggcaccatg gcatcaattg ccttgatacc
     2761 cgtgtggagc gggacgttca ccggagcgcg gtcaataacg ccgtatgctt cgcgctcaat
     2821 aggacggaac ttagtgtttg ggatggggcc tttgccgtcg acaggttcgc cgagtgggtt
     2881 cactacgcga ccaatcatgt cgtctgaaac agggatggag agcaagcggc caaggcggcg
     2941 tacgagcatg ccttcgtaca ccttggtagc atcgccaaga attgccgcac gtacaccgtc
     3001 ctcttcgagg ttcaggatga gtccgtagac cgggttcgca tctttgagtg cttcttcaag
     3061 cgtacggtcc aacgtcgcat cgaagaggac catctcggtc atgacggcat tgtcgaggcc
     3121 gtctatttca gcgataccgt cgcccgtggc gcggacgata cccgtctctt cgctgccacc
     3181 ggttggggtg aatccagcga tctctttctc gattcgtttg atgatgctct ccatagtgct
     3241 aatgcaaggt aataattagt tggcgatgcg gcgatacaag tcgataaggg cacgcttact
     3301 cgaacgatct accagggtgc cgcggcttcg cgcgcgccat ccgcgaataa gcgaatgatt
     3361 cacgtgtgct ttctcggcag taatgccctc agcttttgca gcggcaagag ctgctgacgc
     3421 atccttctcg tgtgcaacct ctacgcgagg agcaactgcc tgacgtcgtg cttcgtacgc
     3481 tttcagttct cccaagattc ctggaagaag cttcatgcgt ccggtagcgg aaagattctt
     3541 caagaggttc tctgccaact tcgcttggtc agtgcccgcc tcgacgaggc gatcaattgc
     3601 ttttgcgtaa gtagcagcct tcatgattat ttctctccag cgaccttagc agcagcgaga
     3661 atggcgaggc gcgcaatctc cttttcgcta tcacgaagtg cttttgctgc agcttcctta
     3721 gagcgtgcct cagcgtccgc tgagatagca gcagcgcgcg cctctgcctc ttttacaaga
     3781 cgggtgcgtt ccgttgcggc agcgtcacgc gccgaagcga ggacttctgc cgcttctttg
     3841 tctgccttct ctaccgttgc tgcggctgca gcgtcagcac ctgcaagctt ctgcgttgca
     3901 cgctctgcgt cttccactcc tttggcaacc atcacgcgac gctcttcaag tactttcaac
     3961 actggcttat agaggaggaa gtacagggca acgatgagta tgccgaagtt caccgcctga
     4021 gcgatgagca gtttccagtc tatgccgaat gcgtcgagta aggcgtccat gcgtaagggt
     4081 tagacgaact tgatgatgaa ggacaccacg agcgcaagaa taccaagcgc ttcggtgaac
     4141 acgattgcaa gaatcatgtt tgagaccacc ttgccggaag cctctgggtt gcggccaata
     4201 gcctgcatag cgccgctacc gataagaccg ataccaatgc cagggccgaa tacgccaaga
     4261 ccggctgcaa tagccatgcc gatgacgcgt gctgattcta gttccatact attgcgtaat
     4321 tatgacaatt tataatttgc tccatcccgg agccgaatgg ataggcggcg ctagtgcgcc
     4381 tcctccccat gcggttccat gatcgcaagc ttgatgaaga acagggtgag cattgagaac
     4441 accactgcct gaatcagcgc cacgaacacc tcaaatgcca atagtggtac cggcaggagg
     4501 tacgccaaga aatagcctgc cacgagaatc atcacttctc cggcaaagac gttcccgaag
     4561 agacggaatg agaacgagat aagccgcccg aggtttgata tgagctcaat taagcccacg
     4621 gcaaaatcta ccggtgattt aaaattaaca aatttacccc cgtacttcaa cagaccgagc
     4681 gtgacaatac cggtgatttc aatggtgaga acggagatga tagcgagcgc aagcgtgaag
     4741 ttgaggtcag cggcaggagc acggagaaat ggtgccatct ccccttcgtg accaaagagg
     4801 atggaaccaa cgcccgggat gaacgcgagc tcgtttgcaa cgaacacaaa gaggaagatg
     4861 gtcgcaatga gcggaaagaa gcgacgtgcg agcttctcgc tttcaagtgt ctcttccata
     4921 aagttaatga cgccttcaaa cgcccattca atacctgcct ggaggcgtcc cggcactgct
     4981 ttaagggagc ggccaaagaa gaaggcaaac acaatgagga ccgccatcgt gatccaggtc
     5041 aacagcaagg agttcgttat cgggaatccc cagacggaga aaagctcctc cgccttaagg
     5101 acgacatgga tagggctcac gtgctctaca gcgtgcgctg cagtcgtggt tgccagatgt
     5161 gctgcttcgg ttgccatgtg atgagagaaa tgaccccctg agggccatat agggacatag
     5221 tagcaagagg tcgctgctat cgcaaaataa gaggccgcgg attactccgc ggccaggatt
     5281 catctcgtgg acgtatatta gtccttcggt tttgtcgccg tcagttcacc tttcaccaac
     5341 atactgattg acagattatc gagaaccgtt tcaagagcgt gctggtaggc gaacatcatg
     5401 ccttcaattt ttccttcgag tcgaatggtt cccaaacgga acttatcgtt ctcatcaagt
     5461 acattattat gcatctcgac cagatcgtta taccgacctg agagagcagc gtttacttgt
     5521 ttttgttctt cgtatgccgt gacgacggtt ttggaaagcg cacgttcgct ttcaaccgcc
     5581 gacgcaagcg tcgtatatcg gtgctggctg attttgagcc gttcgcccaa gtcggcgatt
     5641 tctccttcat actgccgaga cgttttacgc ttggcgggcg ccttgcgtac gacttttttt
     5701 gcagtcttct ttctgggagc catgtggcta ctccttctgt acaagccttt cggcgattgg
     5761 attaaataac aaaaatagca cttacgtcaa atgtggaccc aaggagactc gaactcctca
     5821 cctcttccat gccatggaag cgctctacca gatgagctat gggcccgtta aaaccaattt
     5881 actgtttttt ctgtaatgcc tcaagctcag tgagaatgcg gcggatgtcg ccagtgagtt
     5941 catcaagtga gcgaccttct gaagcaatgc gcttctcttc ctctttctct tcttcggaca
     6001 tttcctcaac gtcctcctga atctcatcca cgtcttcttg aatttcttca atgtcttcct
     6061 gaatttcatc catatcttct ccgagttctt caacgtcttc ctgaatctcg tcgatgtctt
     6121 cttccacctc acggagctgt gccgtgtttc ggttcaccgt catctgaatg aagatggcga
     6181 ggtatatcgc ttctagagag acaatagtgg tgagaataag gagcacaagc tcccaggacg
     6241 ccagtccaaa gagccctacc gcaaaggctg cggcaaacgc aacagtatgg acgataaggg
     6301 aagcaacaga gcccacccac gtagtggcgc gttcaacggg accaatgatt tgtggagttt
     6361 ttgtcatatt cgtcggcaca atagctgtcg ggccgccggt aatcgaaacc gggctctaag
     6421 caccccatgc ttatgtactg ccactatact acggcccgaa tgtttttagg ataacaaatg
     6481 aacgaaaggt tgtgtattcg aaaaaattat taggaaaata cattagccaa agatcgtcgt
     6541 ttctaaaacc tctctcgacg ctttgtcgta tttcgcagca atcaattctg ctttcatatc
     6601 ctcgctcaaa aaatctggat catcgacgag cgcaatcacg tcatcgagag tgacttcttt
     6661 aacggtaatg cgttccccgg gatcaagggc tggttcagta gtgcgcacac agttccgagc
     6721 aatgaatacg tagactgccc aatcaatttt tgttactggc tgatacgcct tccagagcgt
     6781 ccactcgtct gactcgtatc ccgtctcctc cttaagttcc cgctgtgccg cctcaagcgg
     6841 tccttcacct tctttatcca ttctcccggc aggaaaggtg tgaatagcag agcgtgctgg
     6901 ctgttcatcc ttgaggaaga ggagtttttt tccaggaagt gtagggataa cgacaatggt
     6961 gtcgtctcgc ttcactttat cgaatgtctt cgtgctgcca tcgaataatt cttgctccca
     7021 ttgatacaca tcaaagatgt cacctttaaa tacccgctta gcgtggggag ggagcggctg
     7081 acgagactcg ggacgaatga tttccatagg agtagcgtag cattcacttt actcgtttgg
     7141 tatctttcct tggtcgggcg attagctcag ctggttagag cgttcgcctc acacgcgaaa
     7201 ggtcacaggt tcgaatcctg tatcgcccac aagcattttt cctaatatcg ggctgtagta
     7261 taccggtagt acgtacgatt cgggttcgtg aagaccgggt tcgattcccg gcagcccgac
     7321 cctttacgca agtggcctca ggtatgaggc cgtttgccta tcttctgagg ctatacacag
     7381 gccagcttgc caggccgaca aacctcgctc cctagggtat aatgccccgg tcagtaaaca
     7441 ttgactaaat taagacacac tatgcctaga gcactcaacc cggccctcac taagccgatg
     7501 aatctttctc cggaactcga agcggtcgtc ggtaagggcc ctatgccccg ctccgaggtc
     7561 gttaagcaga tctgggttta catcaagaag catgaccttc agaaccctgc taataagcgc
     7621 aacatccttg cggatgacaa gcttaaggca gtcttcgggg gtaagggcga ggtaaccatg
     7681 ttcgagatga cgaaactcgt ctcagcacac cttaaatagt ctcctactac ggaaacaaag
     7741 aaaaaaccgc ccattgaggc ggttttttct tatctagcgt tctcgtatcc agttccgtgc
     7801 tcgatctagg tattttgctg cgtcagcaag ggtgcgggcc accctgtctt gattaccttc
     7861 tgtcttttca tcaaaaatgt acagtgcttc gtcgagtatc ttatacgcct ttcgtacaag
     7921 ttccttaata tctcccttgt tttggtcggt atatgccata taccggaaag tatataccta
     7981 gcgaggaccg aatccgtcgt agtactcaat cttcacatca tcctgccagt cggttcctgg
     8041 gattatggag agtacctttc cgaatacctt agagtcctct tcctggacca cgataggagc
     8101 atagtttgcc gctgcgttct ctgggtgaag gacggcaaag ccgtttgagt gctctaggcg
     8161 cttaataacg gccatgttcc cgatgatagc gaccacacgg tccccgtccg ctacgcggtc
     8221 cgttacttcg acaatgacgt agtccccgtt cttaatacca gcgtcttcca tagagtctcc
     8281 tactgccttt atggccgcaa tgctcgtggc cttaccaagg agcttcttat ccaccgcaat
     8341 aaactccccg aactcatcct gggcgtacac ctccatggcg tcacagccag cgcttgcgat
     8401 gacgggaaca gcaatgaagt cactcggaag atactgactt gaaatagtct tcagtatcct
     8461 tatgctccga tgttgaaatc gatctcgtgc gataaaacct ttcctttcaa gggcttcgat
     8521 tctctgggtg acgcttcgaa gcgaagaaag cttaagaagc gtttgtagct ctgtgagagt
     8581 aggtgattgt ccatgttggg cgatatatcg cttgataaag gcgagcgttg cttcctgaac
     8641 ttctgtgagt gagtcgttcc gagacatgtg tgtactttat catcttgtgt acacaactat
     8701 gcacagaaca tacacaactt ctgttttaga gccttttgag cgtttcctta tacaaagtgt
     8761 cgtgtagttc tacgaggctt tgcatcactt cacgtttttc accttctgct tcctcaagtc
     8821 tctttaaaaa aactgttggc gaagagatat gagctttaga ctcgtgcttt aaaatatagg
     8881 accataagac catactgagt atttctgcct gagcgtttcc atcatagagg tttgagcgct
     8941 gaacgattgc ctccctgcgc gtatgtggtt tgaacttaga cgccgcatgg ctaacggtgg
     9001 tcgtgagttc tatggcgcga tctggaaagc gctccataag aacgccacgc actatatcgt
     9061 tcatctgcgc tatcgtttta ggttcgtagt tatacgaacg ctcgtgagag ggtgcggggg
     9121 cttcagggga catgaggata tggtaccact ccttaagtaa atgaaagacg ggggcgcctt
     9181 atgcaggttt tcgtgaacta agtaatgttc agtacatcaa cttagaactt ctctcatcca
     9241 atagcgacca atacgatttc ttagagtggt agattcataa ggtggttgca ggagttgaaa
     9301 ttgcccttgt aagcttaggg gactgaggga tttgaacctc atttaccgta cttatcttcg
     9361 tactgaagaa cacctatagc aatacatccg agtatgccaa gaaaccacat gtcagttacg
     9421 gctgaatttt catcaattcc tagtatgttc gtagcaaaga aatatgcgcc actgaaaata
     9481 agagttaaaa cgtaaaataa tcccacaatc ttcagggcgg ataaggaaat tttatacaga
     9541 ggcgtgagcc agtccataat aaaattatat cctacctttg gattatttat gctgaaaatt
     9601 taattcagaa tggttacgca tattggagtc tgaaacactt cctgataagc gactttccta
     9661 agagaaacgt catttgtaaa atagttatcc atccgttcgt acatatctct tccattaaag
     9721 tgattgaaag attttttcca cttctttaaa ccccaatcct gcaatttact cacagccgtt
     9781 tgtgccttaa ttaactgtga agcatatatt tgtatgttat ttcgcaagtc tctttcaatc
     9841 attttcgaac gaaattcttc catattgact ttgaatgcac ggctttgaac tctgggatct
     9901 ggattaagtc gattcattgc tatgtgatta tttaaaagaa tcgatgcttc gaattggcga
     9961 tttatgtcat ctataatctt cctgagatct ttggacttat taactaagtc aatggttagc
    10021 gcgtcgacat agccgcttcc agaagattgc tttaataatt cttctggaat ttctgcaaca
    10081 tgaagcagtg gtacaaacgc ataacgaatt gttggctgtc cattgcttat atcatgctca
    10141 gtctgtgaga ttatccttgc gagctgatta ttagaaaaat gagtgagagt tgagtcgatt
    10201 tcggtaagat tagataaagc ggctccaata cttcgatgta gacgaaaagt ttgatcttgt
    10261 ctttcctttc tttcttttgc ccactcggta aggatgaata atattatcgc aacaccggct
    10321 gcaagtaatg ctccgtaaaa atcctgccat tcatgaatga cttctaaaac ctccattacg
    10381 aagatataga gacgcgtaag aagcgttgtg aatgcaagat aaatggtctc catttctata
    10441 ttgtacattc ttacaggtta aaacgcctta aaacctaaac gcgccaatac gaaatgacgg
    10501 aaaccttgac ttcgtgaaca aatgtggacc gtacaggatt cgaacctgcg accctctcat
    10561 tgcaaatgag atgctctacc aactgagcta acggcccatt tctggactcc gataatatag
    10621 catatgttga cagatgtgca tctcaaatcc atccttatcc ctgtgctgaa agtgatggga
    10681 taggtcagcg cagaatagag caggaatgcg tgatagttcg gagtgagata ggcgtcatgt
    10741 tgcgactata tcggcagact ggctcaccgc cagcgtggcg tcttgacccg agaagccttg
    10801 tcgtgtgttc cgtaaaactc tgttccgacg aagtctgccc ctgaggtggt aattcgtcac
    10861 ccccccattg gaggcctgca ttccgcaggc ctcccactct tttatgctga gagcgcgtct
    10921 accttagcag ccataataaa atcgttttct gcgagtcctc gtatggagtg ggtcgtgaac
    10981 tgcacctcta cctttcccca tccgactctc atatccgggt gatgccattc cgcctcagcg
    11041 acttccccta ccttattcgc aaacgcgagc gctttagcaa agtccttaaa agggaaggtg
    11101 cgcgtaagag tagtagcatc ctctgaaagc gtccattcag gaacatcagc atgaagcttc
    11161 tttgcttctt caggagtgag cttgtctata atcccctcac acggcagaca acgctttcct
    11221 gcaaggctag tcatatccct cttctttctt taaagcgttt ttacgttccg tctccttttc
    11281 aagatcatcg aggtcaggct cgccatcgat aagatactcg ggaagtaacc cgtcgtcagg
    11341 gccatgcgcc atttttcttt taaagggtct ttcgttattc ataggtttta gattcctgct
    11401 actgataaat cgacaaggtg cgagaggaag tccgggaatg acacccctac tgccgctaac
    11461 gacttcggga gaagcgattc actcgtcatc cccggaagcg tattggtctc aagatagtag
    11521 atacccttgg gagaaatgat gaagtcagaa cgggagtagt ggcggagtcc cagtccctca
    11581 tgcatcacgc gcgcaatacg tgccaattcc tcttgctcct ttttagagaa aggggcaggg
    11641 catatttccc tactcttacc ggaatactta gcgtcataag aaaagaagtc attttcgggc
    11701 ggcactattt caatagaagg aagggcatag agcttctcac cgcgaaggtt ctgcaccaca
    11761 ccttgtgtgg cttccctccc gcgtatcact tcttcaatga gtacaccgcc tgctccagcg
    11821 gacaatagtt gcattactgc agcatggagg ggcgcgtacc cggagaccat cgagatacct
    11881 accgaagaac cccagcgtat aggctttacc actaccggct ggtggaagct ccgtatcacg
    11941 tctgcaagta tttcctccgc ctgttccgcg ttctgtatga agcggtattc aggcgtaagg
    12001 acgccgagct ctcgtgcgcg ttcctttgcc atcaccttat gcatcgcaag atacgacgca
    12061 aaagaattag aacccgtgta cgggataccg tgacgctcca gaatcttctg tacttcaccg
    12121 tcctctccgt attccccatg gaggcctaca atcaccacat caacgctccg gagcgcctct
    12181 ggaggggtcg tggggcgtcc gtggacatgc cagttgccct gcttatcaat atagatgtcg
    12241 cggacggtat agcgcccgat atcaagattc ttaagaatag cggcacccgt ctttaacgac
    12301 acctcgtgct ccttggaggg tccgccccgt agtactccga cgatggtccc gcgcattacc
    12361 ggctagtata tcaggccttc aatgagcgcc tgcgcgcttt acgatgcaag gttatggaga
    12421 atcggtgcgc ttctgagttc gcgagcacca ctgtagcgtc ggggataccg gcacgaaggc
    12481 ttccaatgac ttcacgcggg cggtgccgct catctttcac cacagcaata actggtatac
    12541 cgataccaag ctccttcaag gtctcttctg ccgccttctt atgcgtcttc ccgccgtcta
    12601 ccacaatagc tttcgggtac tgccactccg ggtggccaaa gcgacgattc aacagttcct
    12661 tcaatgaagc gatgtcatcg tttaacgact ttcctccagc accgcggatc ttaaagctgc
    12721 gatactcctt tttgtccggg agtccgttat caatgaccac catgacccct atcgcattcg
    12781 tccccgaaag gtgcgccgtg tcatatgcct ctactctcgg ctcattctgt tcctctttac
    12841 gatcctcacg gataagggag atgtcctgga catgatcgag cgcgaagagc tgctggcggg
    12901 caagtgcagc gtcttcaaag cgctctgctt tagcagcaag cttcatctgc ttttggagtt
    12961 ccgcacgaag cgtctttacc cggcctgaga ggaacatgga gacgttccgg atggagcgca
    13021 gatacccggt gcgatctaag tcacgcggat agtgccctat ctgcttattg aactccacct
    13081 tcccttgagt aaagcgactg ccttcaccta ccggacgcgg agtgtcatag aagggaaata
    13141 tcttccggat gagacggagc gcctcccgga gctggtagcc tgaaggaaag ggcccgtgca
    13201 tagcacgaag cggtatggag cctatagtta tctccttctt cttgaaatca agatccgtgc
    13261 cgcgtatcgc aagcacacgc ggtatttctt cgtcagtaat gacggcatac aaaaatgact
    13321 tatcgtcctt acctatcgca ttggcctttg gcttatgttc ccgaataagt gcggcttcgc
    13381 gcacaagtgc ctctaagacc gtaggagtag tctcatgcat aaggccgtca gagagggtaa
    13441 ccatgtcgac gatacgcggg ccacgggtcg cgataaggtc cacatcaaag tacgaacgca
    13501 cacgatcacg cagcgacgtg gccttaccta catagagaat cttctttgat ttccctttcc
    13561 cttgggtaaa gagatagacg ccggggacat ccggaagatc aagtttagtg agataggccc
    13621 gttccatata ggtattctag catttcttaa tttgacttac aaaaaatagt acgtattatc
    13681 acatcgaaac aacgtctgga ggaggcgaat gcttacctat ctaaagtgcg cttgggtaaa
    13741 tccttttacg cgcttcagtt acttgattgt ttttcttggt ctactgattc tgattgtatt
    13801 cggttggcca ggactcattt ttatcgccct agggctttgg cttttgtgga tgctcaactt
    13861 tggagcaccg acctatgttg cctatcggat gaccagtaag cgcttacgta gagagaatgg
    13921 catcacacca tttttcctct atgcgatgac tgcatccgga ccatgcataa tggaaggctt
    13981 cacccttgct gtcagtgagc agctggctag gataaaaaag agcgaggcgc gacgatgagt
    14041 cgcgcctttt ctttacattc atttaatttg acattttagt atttcaacgt attttaatag
    14101 aagaagttcc gatgtgcggg cttcttaggg agccggaagc aatttccggt aacgacatga
    14161 gtgatacggt actcaagatc gaaaaagtcg ccgcattaaa acaatgcatt ctggcggctg
    14221 cggcacttgc actatctgtc tggggatgcc tgcttggcat gtcctacaac cacgaggacg
    14281 ccggactggt actgctcgtc tcgagcctct accacgtcgt caaactttac tgcgaagttc
    14341 aaaacctcta cgcgttgaag caggtatgtc acgcgtaaaa accggtgcgg taggacggga
    14401 gcagttgctc tcgttttacc cgccgatttt ttatttcttc tttttcttac gtagcaactt
    14461 cgaaagatac tctccggtat gagacccttc agtatctgca acctcttctg gagttccctt
    14521 tgcaattacc ttgccgccgc cggtgccgcc ttccggacca aagtcgataa ggtagtcaca
    14581 gcttttaatg acatcgaggt tgtgttcaat caccactacc gtattcccgc ggcgcaccaa
    14641 ctcctgaagt atttcggtaa gcttgcgcac gtcgtcatag tgaagaccta ccgtaggttc
    14701 atcgagaaga taaagggtct tcatcgtggt tggacgatag agctctgcag atattttcac
    14761 gcgctgcgcc tcaccgcccg aaagcgtcgt cgctgattgt ccgagcgtaa ggtactcaag
    14821 accggtggag ttaagggatg ctagacgctc agaaatagcc ggaatatctt tgaagaagac
    14881 ttccgcctcc tctaccgtca tctggagtac gtcatagatg ttcttaccgc gatacgtgac
    14941 ttcaagcgtt tctttcataa agcgcttacc gtcacagacg tcacactgta cgtacaccgt
    15001 cggcaagaag tgcatctcca ccgcaataac cccgttaccc tggcacgcct cacagcgtcc
    15061 gcccgcaacg ttaaaggaga aacgtcctgg cttccagcca cgagcacgtg cctcagacgt
    15121 tgctgagaac aggtcacgaa tatgagtgaa ggcacccgta taggtcgcag ggttcgagcg
    15181 cggggtgcgg ccaataggtg actgatcaat aagaaccacg cgccctacgt actcagtgcc
    15241 cgtaatagaa gcgacatgct ctggcttcgt ctcacgacga ttaaagcgtg ctgcgacgtt
    15301 cttatggagg atgtcgtaaa ggaacgtcga cttccccgag cctgaaacgc ccgtgatacc
    15361 cacgagtttc ccgagcggta cttctacatt ctggttcttg aggttatgga gcgttgctcc
    15421 ctttatcttt attgaacctt tctcgctcgt acggcgctct tcgggaacgg caacttccat
    15481 atctccgcga aggtaatcaa gggtgagcga gcctgattcg ttctttggcg cacgcaggag
    15541 ttcatcgagc cagccagcaa cgactacgtt tccaccatgc acgcctgctc ccggaccaat
    15601 atcaacgaga taatcagagg aaaggatagt gtcctcatcg tgctcgacca caagaatggt
    15661 gttaccaagt tctttgagtg ttaggagcgt cttgatgagg cgatcattat cgcgctgatg
    15721 cagaccaatg gtcggttcat caagtacata gagcgcaccc acaagcccgg taccaagctg
    15781 agaggcaagg cggatacgct gtgcttcgcc ccctgaaagg gtagcggcag agcggtttaa
    15841 ggtcaggtag tcgagaccga cattcagcat gaaatccaag cgggacgtaa tttcacgaat
    15901 gatagggaag gcgatttccg ctttcatatc agagagatgg aggtcatcca tgaagttctt
    15961 tgcctctcga atggacatgt cagtgatatc aacgacgttc ttgccgagcg tcttgtctga
    16021 ctttcccttc aagtacacgc gaagcgcttc aggacgaagg cgcttccctt cacagaccgg
    16081 acaaatggtc tcggagaaaa gagattccgt accaagaccg ttacagtttg gacacgcacc
    16141 atacggactg ttgaaagaga agagacgtgg ctccacttca gggaacgatg caccgtcttc
    16201 agggcaaata aagcgagcgg agagggtttc cgtggtcccg tccgcatgcc ctatcttcac
    16261 gagtccatcg ctttccttaa gagcgagctc aagcgcttct gagagacgtt cacgtgcagc
    16321 gtcaggggtg cgtatgaatt cagatacgaa aataccatcc accaccgcat caatatcgtg
    16381 ccgcttattc ctatcaagcg tcaccttctc acgaagcccg tgtactttgc cgtcgatgac
    16441 tgccttctca tatcctttcc cgagaagatc ataaaggagc tggtagtact cccctttgcg
    16501 tcctacgacg acgggcgcgt aaatcgtcac catcgtctta tcaacgacaa cgcccttcac
    16561 cgccattgag tgttcagtgc ttccggaacg cttcttgatg cgatccatga tgagacggat
    16621 catttcttcc tttgaaagct tctgaatcgg aacgtcgtga tgaatacagt acggctggcc
    16681 cgcacgtgca tagaggacgc gcagataatc gtagatttca gtaacggtag ccaccgttga
    16741 gcgagggtta ttggaacgag acttctggtc tatggaaata gctggagaaa gacctcgaat
    16801 ctcatcgacg tccggtttct gcatctgatt caagaactga cgcgcgtacg cggaaagact
    16861 ttcgacgtag cgacgctgcc cttctgcgaa aatagtatcg aaggcaaggg acgacttccc
    16921 tgaccccgaa aggccagtga taaccgtcat cgtgccgcgc ggcagatcaa cgttgatgtt
    16981 cttgaggtta tgggtgcgtg cccctttcac ttcaatccaa tcatcgccaa cgtactgcgt
    17041 gtgcgcgtct gttttctttg atttaggcat ggatatagga gttcctgtcc gtggcgggac
    17101 aggtacaata tgaagtatag caaattgcct tctccccggc gactacacag gtttacacgt
    17161 ttggcgtgat ggtacccagt actttctcaa ttcttccgac aatctcttcc actgaaactt
    17221 ctgccttcac caagtattca gttgcgccaa gcgctaatgc gcgctctttg tctttatcct
    17281 gaccaagatt tgaaacgacg atgaccggaa catctttgag agcgtcggta cgcttcttga
    17341 tggtctcgag taccgcaaac ccatccatgg tggggagcat gaggtcgagg aggacaagcg
    17401 ccggcttgtt cgcttcaaac aaggcaagcg cttcgttacc gtctgaggcg tacaaggtct
    17461 ggtactttcc agcaaaggtg tgtcccagga ggttcttgag gatggggtcg tcttcaacga
    17521 cgaggataaa gcgggtttca ggcataggag aagaatacca cgcgctacgc gagcggaagg
    17581 gacagggtaa aggtcgtgcc agggccctcg gtgtttgcgg caaaattaag gtctccgcca
    17641 tggtcagtgg ctatggtgcg agcgatatag agcccgagac cattaccctg gtttttgacc
    17701 gtaatcgcgt tctgagcgcg atagaagcgc tcaaagatgt ttgctctatc ttctcctgga
    17761 ataccgatgc cggtgtcctt cacgcgtaca tacacgcgcc ctccgcctgc ttcgctcgat
    17821 acccgtaccg tgcctcctgc aggggtgtag agaatggcgt tctcaataag gttattcagg
    17881 acccacttaa tgcgttcacg atccgccttt gcagcaggga gtgcttctga agcctcatgc
    17941 gtatagaaga gagagatctg cgtcttttgc gccaagggtt cgaagcttgc cacgatttct
    18001 gttacgagcg cattcatatc cgtaggagcg aaggtgtatt tatacttccc tgactcaatg
    18061 gcggagatat caagaagcgt gcccacgata gccacaaggt cttttgactt ccccacagca
    18121 ctttcgacga gcgccttctg gctttcggtg aggttctcct tctgaagggc ctcaagcgcc
    18181 cagcgaatgc ccgttaacgg ggtacggaac tgatgcgctg cagtagagat aaagtctgac
    18241 ttcacacgcg agatgtccgt atcatgctta tgcgactcct caaccctatc tataagccca
    18301 tcgaaggata cggcaagtga acgtatctct ttaggcgcct catctatccc atcaatagtg
    18361 gtacgagcgc cactcgtggc gtacccgtcc atcgcgcgag tcaactcacg taacggtttt
    18421 gaaacgaatg aaccaacaac gaagaagata gcgacggaga gtccgagcgc gagcacaaag
    18481 agtgcaatcg cttgcatcat gaacagattc atcaatgatg catctacctc aagcgcaaga
    18541 tcatagggca acagcgcgct tatctgccca cgtaaataca aaatacttaa aagaatgacg
    18601 gtagcggcta ccgcgcaggc aataaataac gttatgccta aaagagaacg gaaactcatg
    18661 caggaatgat agcatctgag taaaggcaaa acgggatccc gaaggggatc ccgtttgtac
    18721 ttatctaaca attcgacagt agcgtgtctc aagcagatac acctttacgt tcggctcttt
    18781 caagatagat accgttcgat accagtgcgc agcatcagga cactcttcat cgagtacgga
    18841 gaggaaatgt tctttgtcga taacataccc tcgccacccg tcatcaggaa tttggttccc
    18901 aacagagtcc accactttaa gtgctggaac gcttacgaac gaagagtagg tttcaaaaat
    18961 acgaccaacg agtagctgtc ggacgtattc agggattgtt tcgctcggaa agagagcaat
    19021 aacttgaatg cgtggcattc caacctccaa ctaatccaaa ggcactgtac cgctatagct
    19081 tataaaagca attacgcctg tggtacgcta ctcaaagtat gaaaaatcct tatttctggt
    19141 gggttgtttt agtaacactc gggtttgctg ttgtatacag tttacctatc ggtggtactg
    19201 gaggcatagt gatactccat ttactctgga agatggtggc gccattcatt atggttgccg
    19261 gagtcctcac tcttctttac atgattttta ccggtagtcc gaaaaagtaa ctacgaaaag
    19321 cgctttcgct tacgttctac tttcttctta gggccattcc cctcaagttc atagagctcg
    19381 tctcgaataa gggcggcggt ctcaaagtcg agcgcttcta ctgcttcacc catacgctgc
    19441 cgcatctcct tcacaaagcg ctccggatcc ttttcataga ggcgcttatc aagcgaaagc
    19501 atgtctccca ccgttttatc gtgctcactg cgcatactct cggcaatgtc tcgaatctcc
    19561 ttcttgatgg tttgtggcgt gatgccgtgc ttttcgttat acgcgagctg tattttgcgg
    19621 cgacgctctg tctctccaat agcgcgtgtg agtgaaccag tcatcacatc cgcataaagg
    19681 atgacgcgtc cgagcacgtt acgcgctgcg cgccctatcg tctgaatgag agaggtttcg
    19741 cttcgaagga agccttcttt gtcggcatca agaataccca ccaactccac ctcaggaagg
    19801 tcgagtcctt cacggagcag gttcacgccc accagaatat cgaagttacc cttacggaag
    19861 ttcgtcagaa tatctatgcg ctcaatggtc ttaatatccg aatggaggta ctccgctttt
    19921 atcttccgtt ccttcaagaa ctccgctagg tcttctgcca tttgtttggt gagcgtcgtt
    19981 gcaatgacgc gtccaccacc attgatggtt ttctctgctt cagcgataaa gtcagctacc
    20041 tgacctttgt agtcaccctt ctcgttgatg ggacgaatga caagctcagg atcgatgaga
    20101 ccagtagggc gaatgatctg ttctgcaatt tgtccttttg gcggtacgga atgctctcgc
    20161 tcatacggcc caggagttgc tgaggtgtat atggtttgcc ctacgcgctc ttcaaactct
    20221 tcaaacgtaa gcgggcggtt atcgcgcgca gacggcagac ggaatccgaa ctcaaccaaa
    20281 ttatccttac gagaacgatc tcccgcaaac atgccgccta tctgaggcac ggtgacgtgt
    20341 gactcgtcga taacggtcag gaagtcagct gaaccatcct tcttatgagg aaaatacgaa
    20401 agaagggtgt gtggcggctc ccctgcctta cgcccatcaa agtgacgcga gtagttttca
    20461 atacctgagg tgtagccaat ctctttaatg agtgcgaggt catgcatcgt gcgacgctta
    20521 atgcgctccg cctcaagcaa cttctcttca cgcttaaact ttgccaaggt ctcatcaagt
    20581 tcaagacgaa tgtcctcaag cgctctatcg cgctcatcat cacttgtcac gaagtgctta
    20641 gccgggaata taaacgtcgt gtcaggttct gcgacgagcg cacgcgacac ggggtcaagc
    20701 tgctgaatgg taccgatgac tccaccatca aaggtgacgc gatagataac acgctcgttg
    20761 accggcatga actccaccgt attccctacg gctcgaatct ttcctggctc gaggtccgca
    20821 ggcgtacgtt caaaatagat gccgatgagc ttacgaataa gggcagcacg gttctcttct
    20881 gcaccctttg caagctttac atggaacttc tcgtactgag ccggggaccc gagtccgtaa
    20941 atagcggaca ctgacgccac gataatcaca tccttacgcg tgagaagcgc ctgggtagca
    21001 gcgtggcgca aacggtcaat ctcagcatta atctgtgcct ctttctcgat ataggtatca
    21061 gagtgcgata tgtacgcttc tggctggtag tagtcgtagt aagaaacgaa gtagtgcacc
    21121 gcgttctccg ggaagaactc gcgatactcc tgcgcaagct gtgcggcaag cgtcttgttg
    21181 tgtgcgagca ccaacgtcgt cttctgcgcg ttattaatga cgtttgcgat agtgaatgtc
    21241 ttacccgttc cggtagctcc aagaagcgtc tgatgcgtaa gaccgtcagc gacgccttta
    21301 gagagcgctt taatagcctc gggctggtcg cccgccggct caaaaggaag atgtagtttg
    21361 aatcctgcag acataaggga aacaagccat tataccaatt acaaatgaaa aacgcccgaa
    21421 gccgaagctt acgagcgttt tctcacaggt acatatcgtt gaggatggtc ggggcggacc
    21481 ttctggtccg cccctcccgt cgtgccggcg tcgcttagct tgacgcaggc gccgttgagc
    21541 ggcgcgcacg ctgcagttga tcggcgaacg gcagaaggcg atcgatcgcg gccggatggt
    21601 tcttggcgag acgttcaact tggtctttga acgccgggtc gccgagcatc accgaaaccg
    21661 tcgttacaaa cacgccgccg cggcgctcgt cgcgcgtgat cgccgtcatt tcatcgaacg
    21721 gatgattgcg cttcgtgtcg ttgtagaacg tcgccaaatc gttgccgagg tcggcgtctt
    21781 ggttcgcgat ttccggaaca gaaagaacgg cggcaattgc cgtcaaattc aacattcgtg
    21841 ccttcacggt cttcattttc aacatcctca aaagcaacga gcccttgcgg gtctcgtacc
    21901 tctccactct ggagacgatg gattgatact agaatatgga caatatatgg tcaagtgttc
    21961 ttataaaaaa cgcggtgacc agtaaattgg ccaccgcgtc tctagtatct ccgcatggag
    22021 cgtattattc gccgccggtg gcgccgtcgc cctccggttc aggcttccag tcggcgtcct
    22081 tggcgcgcat catcgcggcc cataccttct cgattccttc ttctgattcg atgacgaatt
    22141 cgagtaaggc aacgtagtcg gttgcaatgg cgtggactgc tttgctgacg gtaagcacgt
    22201 cagtgtagtg gtcttcgcgt tccttgttcg aacgaacaag atgagcgaag gtttcacccc
    22261 aatcaccaga ctccatctgt ttgagtgtct cgccgagatc ttcatcgatt tcggaaacac
    22321 cttctgaggt gaagaagtcg atgacttcta ggaacttcgt cgaaagcgct atcggttttg
    22381 ccaaaagcgc ttcgacctgt ttaaggatgg cttgcgttgc atccaccgtt tcttcttggt
    22441 cctgccgagc gacagcgcta gcggaattgg acatattcgt ctcctcgaac aagcattcca
    22501 gggaactccc cgaaagctga tggaaattag cacattttaa taaagggtca aaacaactac
    22561 gtgcctttat agtcccgcaa gaaatcagca cgatactgtt caaacgttcc gttatgaaga
    22621 gcgtcacgtg ctccatccac gagcttcaag ataaacccaa ggttatgcat ggaggcgata
    22681 acggagccca gcatctcttt tgaacgtacc aagtgcgata catatgcttt cgtgaagcct
    22741 tcagtgccag aaacaatcgt ctcagggtca agtggactga agtcattctt aaattccccg
    22801 ttccgtagat gcttaatgcc acgacgcgta tagatggagc catggcgccc aatgcgcgtt
    22861 gccgcaacgc aatcaaagag atccatacct tcagcaatac ccgtgagtac gtcggtaggt
    22921 tcccctatcc caaggaaatg gcggggtttc ccttctggaa gctcaggaat agcggcctga
    22981 agcgcgccat ccatatcttc cttactaaac gacccaccaa taccaaaccc atcaaagtcc
    23041 attgaggcga tagtgttcgc gctttctttg cgtaagtcga ggtgacgtcc tccctgcaca
    23101 ataccgaaaa taccctgcgt cttattcgct gcaagattct ggcgatgcgc tttcagggaa
    23161 cgctccgccc agcgatgcgt acgatacatc gcttccttct gatattcata ggactctgtg
    23221 ggagcggtgc actcatcaaa agcaaagaac atatccgcgc caagcgcatg ctgaatctct
    23281 atggagcgct ctggggtgaa gcgatgcagc gtgccatcca agtgcgaggt gaaggtcacg
    23341 ccttcgtcat caat
//
LOCUS       DAORIN010000002        18947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_469_length_18947_cov_5.861158, whole genome shotgun sequence.
ACCESSION   DAORIN010000002 DAORIN010000000
VERSION     DAORIN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18947
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_469_length_18947_cov_5.861158"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PK109_00175"
     CDS             <1..197
                     /locus_tag="PK109_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRH23996.1"
                     /translation="LITHGENGMLVRSGDCVALSQAITAVLADEPLRARLSEAAKTSA
                     ERFSLGTLGKETAALIKENI"
     gene            194..1348
                     /locus_tag="PK109_00180"
     CDS             194..1348
                     /locus_tag="PK109_00180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRH23997.1"
                     /translation="MKALFVSNDPSIFVSGSATRARMREYAAAIGTLHILSRAPKGAK
                     EEVEGNLILHPIHAHKLLTPFTLARRARALIAREGIEIVSAQDPFEQGWAAARAVRGT
                     AAKLHIQVHTDFLTPWFVRSGVFRSMRIHMPFLNRFRVRLAKKVLPQAHGIRAVSKRV
                     ADSIVAAYGATVPVPVVIPISVGSIVPQAIPLPPHEFSFAFMCSGRLEPEKRVEDILM
                     ALGKIAHSYPSVGLVVVGEGSERKKLEKLTKKLGLSSRVLFMGEQGKDAWGLMKSANV
                     FVQASAYEGYGRTLIEAALARVPIITTDVGIVGEVLKGYEQVLATPPGDTANIAAHMH
                     AMIGDHQARLTLAMNAEKAAKDHIAAFADPATHIAENLSETLKKGADRAY"
     gene            1364..2332
                     /locus_tag="PK109_00185"
     CDS             1364..2332
                     /locus_tag="PK109_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRH23998.1"
                     /translation="MNIVTIGGGTGTFVVLSALRRLPNLSLSAIVSSADDGGSTGRLR
                     DAYGFLPLGDARQALVALAEDGPVLRDLFAYRFAKGDIKGHNLGNLFLTALTDLLGSD
                     TKALEEASRILRVSGRVIPASEVPATLMATLADGSTLRGEHVIDEPNANRSAINQVYL
                     ETDVSASPSALEAIREADVIILGPGDLYASTVAPLLPAGMREAIASSKGKLIYIVNLF
                     TKAGQTGGMSVSDYLREIERYAGRAPERVLIHKNGGFEANVLERYAEEGESPVTDDLG
                     EDERASRASVASVHIVPPVPEDPVPRSLARHDPEKLAAALAPLITL"
     gene            2329..3201
                     /locus_tag="PK109_00190"
     CDS             2329..3201
                     /locus_tag="PK109_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH23999.1"
                     /translation="MNKKIGFIGQGWIGKNYADDFERRGYDIVRYALEAPYNVNKEAI
                     AACDIVIIAVPTPTTEAGFDASIVQGVLPLVGVGKTAVIKSTLIPGTTEQLQAAFPDR
                     IIMHSPEFLREAHAAEDAAHPDRNLIGIPVDDAVHREAAEAALAVLPPAPYAKIMNAK
                     SAEIIKYAGNVFLAMKVVYANILYDLAAAEGAPYEEIKDAVGADPRIGSSHLSPVAAS
                     GHTEKAGRGAGGHCFIKDLEAFRRLYDELANDKEGSALLAAMVRKNNALLIASGKDLD
                     LLGGVYGPNYDVLP"
     gene            3185..3790
                     /locus_tag="PK109_00195"
     CDS             3185..3790
                     /locus_tag="PK109_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24000.1"
                     /translation="MMYFLDFDRTLFDTDAYNASLADEPGCAPFREDILKVLSQKRDD
                     TLVPPAERARVWGLVSEAIKSGALSFPPGYLERFLYADVAETLRSLGNEAVIITYGEE
                     ARQRLKIESALANAVRVTVLYTGDLSKANYLSSWPGYYGQEAAMVEDRTAELEALAEK
                     FPKMKLFKIERGARPPQSIEGAPSFESEGRWKTIHSLAELP"
     gene            3787..4821
                     /locus_tag="PK109_00200"
     CDS             3787..4821
                     /locus_tag="PK109_00200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRH24001.1"
                     /translation="MKLLVVTQVMNQEHPVLGFFHHWVETFAPHFEHIHVICLEEGVH
                     ALPQNVTVHSLGKEKGASRFMRVVKFLQLSWGLSKDYDAVFVHMNPEYLALSGDFWRF
                     TGKKVGLWYNHEVGSIWLKLAQPIVHTLFYTSPYAYPARYKNAQKMPAGIDTTLFTSG
                     SAVRRADSIYFQGRISPAKRVHVLLEALRIVRRTRKSATVTLVGPEDAAYGATLRTVF
                     KDLIEAGAVTFMGPKRNEETPALYGAHAVSVNLTADGNFDKTVLESLACETPAIVSSR
                     AFEGLVPDEWRIAENDSEALAVALLRALSLSAAQLHTLGANCRNLVVQKHSLTSLADE
                     LAQAYNHRAH"
     gene            4833..5468
                     /locus_tag="PK109_00205"
     CDS             4833..5468
                     /locus_tag="PK109_00205"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRH24002.1"
                     /translation="MTILRKAEHQALAGFPLSGKVLDLGGDTRSDYRNMFSGDYAITT
                     VNMLPEAKPDILADLEEPLPIEDASYDAVLLINVMEHVFEYRALLLECARVLRPGGTI
                     VIIVPYLFPYHASPHDYHRYSAEALSRALLISGFKDDVVRPLGSGIISARVVLTERLL
                     PGAVQSVLALILHPLAGMLDGLFSSLARLLRKKYSPSDYALGFLASAKKAV"
     gene            5479..6561
                     /locus_tag="PK109_00210"
     CDS             5479..6561
                     /locus_tag="PK109_00210"
                     /inference="COORDINATES: protein motif:HMM:NF015007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exostosin family protein"
                     /protein_id="HRH24003.1"
                     /translation="MEPVRLYVGPDYKEPQLYTYVELLFPFWGVAAKESMPYVRAAAT
                     QYQYSKNDFVLVDDIKDADYVLMPYHYERLMAVNPEKVARIVREAQEAGKPLLIDGSG
                     DIEHVIDIPNTVVLRVSQYRYAVKENEITVPFLAEDLLESFRGGIVELRIKMQTPSVG
                     FTGWADISFITRLKIFVKEFSITLGALFDEKRGAEHKGLLFRARALKALQKTKGIVNN
                     FTIRKSYSGHSATIQGAVKDNRQAFVDALIGSDYALVVRGDANASVRFYEALSLGRIP
                     LFLDTACVLPLEDRINYRDFCVFVDWRDTDQIGENLVKFHQSVTSERFVEMQHRAREA
                     YQNYLRIDAFSAHLADMLRARLTIQK"
     gene            6558..7367
                     /locus_tag="PK109_00215"
     CDS             6558..7367
                     /locus_tag="PK109_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24004.1"
                     /translation="MKHALIAFLKQVNAMPFRFSMKHYQVFGSATEALKDQELNSAQS
                     WDALRETHPFFSISENRDEWLAASELAVAKDGQDTALAARADEVVALIQRKGFDRVFS
                     TGVGGAALEYQIKKKMPQLPVVCSDYSAETVRRLQKVFTESDGVIEFDILKGDWKAVE
                     RQYLGTNGLCIMYRLDAGFSDDQWHIIFDVMQEAGVTNVLVIPTGTLTMLSIYNRKKR
                     ELLWALKHIPVVFSGYLRTKHRFISHWDEWYDTEEATFGGLKGFLLKKRAR"
     gene            7463..8671
                     /locus_tag="PK109_00220"
     CDS             7463..8671
                     /locus_tag="PK109_00220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012559609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRH24005.1"
                     /translation="MCASTNLVEYLDLGNHPPSDQFRTEEELTLPEVRYPLRVLLCQN
                     CGLSQLSHVVDPRVLYQHDYPYESSMTKTGKLHWDGFAESVIKRLALPENALVVDIGS
                     NVGTLLESFKAQGMKVAGIDPAPNIVEKANAAGIPTVCDFYGSVAANKVKAQHGSAAV
                     IVGTNVFAHIDDLDAVMEANKELLAADGVFAFESPHFGPLVEHLEYDTIYHEHLSYLS
                     LKPVVAFAKRHGLEAFAVEESAIHGGSFRVYIGEAGKRPIDASISNLLMREEREGLHL
                     LTTLEAFALRVAENREALRRLIEKLLADGKKIAGLSAPAKGMTLLNYCGFGTRELAFL
                     TEKSTLKIGRYSPGGYIPIREDNALLDEDVDYALLLAWNFAPEIIKNTKAFSDKGGKY
                     IIPIPTPKIV"
     gene            8682..9065
                     /locus_tag="PK109_00225"
     CDS             8682..9065
                     /locus_tag="PK109_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24006.1"
                     /translation="MEITHTEIAHTDDRGEIRDILAHEDIDAVTYITYTKGAVRANHY
                     HEHTVQWDYVISGTLESYSRTGFGAPIEMKIIGPGDLVKHPIGEHHALRALEDCVTLA
                     LAEGPRQGDEYENDVVRLKDDEKLI"
     gene            9071..10195
                     /locus_tag="PK109_00230"
     CDS             9071..10195
                     /locus_tag="PK109_00230"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRH24007.1"
                     /translation="MHVPIGKPLFGAIEREYVNDALEKTEISGFSGGYTSKFENAFAA
                     FCGTKEAVTASNGTTALHLALAALGIKEGDEIITQSFTNMATFFAVLYQNAIPVPVDS
                     EPDTLNINPALIEAKITSRTKAIMVVHIYGHPVDMDPIMALAKKHNLFVIEDAAEAHG
                     AEYKGKRVGSIGDVGCFSFYANKIITTGEGGMVTTNDSALAEKMRSLKGLAFGKENKF
                     MHADIGYNYRMTNVQAAFGAAQMTHADELVAKKRSIAGYYLEHLAGIPGLVLPIEKPY
                     AKNVYWMFNILLDGPLKGKRAEFMRALAERGVETREDFIPFNDQEIFIKRGLTQTSDC
                     PVASNAGVNGFYIPSGTDINDEEQAYVVAQIKEVVTTLTS"
     gene            10199..11488
                     /locus_tag="PK109_00235"
     CDS             10199..11488
                     /locus_tag="PK109_00235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRH24008.1"
                     /translation="MRILITGTTANSMPPPYAGVPKLILATARLWREKGHEVAITWRY
                     KPKDADDLNAGATYFFEYGGSDQPNKLQKVGFLLKYFLKNPSLYFSLLESYKSICPHH
                     YDEAYLYAAYGVYLDGIYESWKPDVVLSQAALIKSFMAAELANRRHIPIAFDVYSEVR
                     DFSMGVNRFLSDVERKRYWMGLLGRADLVLGMDNCSVELRAYAREGTLKEFWDTGDYE
                     FFSQPIPETRTQLREYFSLPSDMFLVSAVGSFELRKGHDHLIMAVARLAKQGLNIGAV
                     ICGGQGNRQKWRDVAKEAGIAEDRLFFFSNISELELVKLHHTVDVYTNLSNSPRMCGY
                     DLALIEAMSAGLPVVVYDNGALPKVIQGDSTNGYVVPMNDIPAAAEAMKKLYEKTPEE
                     RKAMGKISAAFASTIDLRKTTSLKLGWLEELVVHSGK"
     gene            11485..12483
                     /locus_tag="PK109_00240"
     CDS             11485..12483
                     /locus_tag="PK109_00240"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRH24009.1"
                     /translation="MKRAFITGITGQDGAYLAKFLVEKGYKVFGGYRRSSTRNFWRLH
                     YLDVKKDIELIPMDLIDQTSMIMALKKADPHEVYNLAAQSFVGASFDEALSTGEISGL
                     GVTRMLDSVRIVNPKIRFYQASTSEMYGITAPPQSETTPFAPQSPYAAAKLYGHWVTG
                     NYRTGYGLHASSGILFNHESPIRGIDFVTKKITDGVARIKMKYINEMYLGNLEAKRDW
                     GFAGDYVEAMWQMLQQDKPDDYVIATGETHSVREFCEAAFSYVGLNYKDYVRVKEEFK
                     RPLEVPVLLGDPSKAKAKLGWTPKVGFEELVHMMVDADMDKYQNPTKHSVQFLKHI"
     gene            12486..13217
                     /locus_tag="PK109_00245"
     CDS             12486..13217
                     /locus_tag="PK109_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24010.1"
                     /translation="MGEEAPSGSNAEKLARTKYISATAFTLLPEMRTLFRGGKLTSES
                     GWRNVAEHCFASAVAADIFTDALNLTAEEGRALTSTSAVSDWNKRLEKRPGDFSNEDK
                     AIAQELLSQIAPDKKLLAATHPEFFVRAYEDPQNIPFLENLMLYIDNTTDESNWVSFR
                     ERLPKVMNRWKDLDETYSPRLGGKKFVEFAIEHDASFQEELYVRLKAALEAKGREAPT
                     SPDDIPAWLNRELDRRVKEATRSST"
     gene            13262..14353
                     /locus_tag="PK109_00250"
     CDS             13262..14353
                     /locus_tag="PK109_00250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRH24011.1"
                     /translation="MRICYLSTARIPDDWGHVVQIMKMCEAFAKAGNDVELVVPRRAG
                     RRNENPYAYVGADNVFRITKLPCIDISATGEGKFLYWLRTFSFLLVARLYTLIKKYDV
                     LYTREPAASLFFKDFVFEAHSVSKSVESISKKACKVVAITSFIKDELVTAGVRAQNVL
                     VAADAVDLNAFKNAESKEKARTRLGLPQDKKIALYLGRLDSWKGTDVLYGAADLLPED
                     IQMVIIGVEPAQLDTLRAQHPRIIFFALRPYKEVADNQAAADVLILPNTGKNVMSSRY
                     TSPLKLFSYMASGIPIVASDISSIREILNESNAMFVTPDDSMALAQGIKKTLTDLSVS
                     EKRAEKARDDVELHTWDARASAILSFLSI"
     gene            14350..14982
                     /locus_tag="PK109_00255"
     CDS             14350..14982
                     /locus_tag="PK109_00255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRH24012.1"
                     /translation="MKKLTRGSIDAFLNKYASDRKTLDIGAASGDHPMFSNSTALDID
                     PARNPDVVGDAHNLPFPDASFDIVVCSEVFEHLTNPPKAAAEMHRVLVPGGMLILTTR
                     FLFPVHDAPTDYFRYTPYGLRHIFREWEIIEERCESDSFSTIAVLLQRIIFQSELKGG
                     KVTKGILWLFVLFFSQLDFLTMKRYGEIGRKESIPSLLSSGVYIVCRKSE"
     gene            14998..15720
                     /locus_tag="PK109_00260"
     CDS             14998..15720
                     /locus_tag="PK109_00260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRH24013.1"
                     /translation="MNRETIQEDEYEFPYHHIPTHAPFAQSRLLFWGYEYEAYLEEIL
                     AVLEGMSFKSLIDIGCGDGRLLSELVKSSNARLVGTDFSEKALAFARAFSASVEFQRD
                     IPDKETFDVLTLIEVLEHIPPEEIPTFLNTLRGALVPGGKGVITVPSVALPVNRKHYQ
                     HFSAESLTETLSPYFVIDSITHLNTVSLGSKIIARLLSNRFFILNHSGMREWLYGIYK
                     KHHLRAHPGKGARLMAIVTPRA"
     gene            complement(15704..17002)
                     /locus_tag="PK109_00265"
     CDS             complement(15704..17002)
                     /locus_tag="PK109_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRH24014.1"
                     /translation="MIGEMLSSLRERVFSLLKWSERYTKTDMVYLAGNGFWLAVGQVG
                     IGLIALGVSVAFARFVPKDVYGNYRFLLSIFWILTAFSFTGISTVLARAVARGESGAY
                     ARSLRLSLLWSIPMAIIGLGTSIYYYLNENTVLAAGALVIAIIGPFMQGAYLYGSYLE
                     GKQAFRENAIGGIVLNLVPALALLASMFFIDDPVIFLLIYLGTSVLTGFVVSYVVVKK
                     QAVSLKTQSKGFGTLSAHFSLMNILNTVSTQVDRILVFHFLGAVELAVYAFATAIPDQ
                     IKGLFNSVATLALPKFVKRPLEEVKSTLVYRLLGLSVIAAVAAMIYAFIAPFVFHILF
                     PAYSEAIFYSVLYAVTLIPVGAVVPLALLEAHAAKRELYIYNVAGPLFQIAILLILIS
                     TMGLLGAVIARIIGRIFFLVLGIILTYSYSARIRKLKLVV"
     gene            17130..>18947
                     /locus_tag="PK109_00270"
     CDS             17130..>18947
                     /locus_tag="PK109_00270"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRH24015.1"
                     /translation="MALTTTARWTILGVLFVIPFLPLFVANGMFFPFISSKGFAFRIL
                     IEIAAGAWLLLMLADKRYRPQFSWPLAIYGAFVLWMFIADLFAANPHKAFWSNYERMD
                     GWVTMIHVFLFFLVAGSFLSVEKLWKRWWLTLLGASALVSVYGLWQIFGLAQIHQGGV
                     RADASFGNAIYLAVYLMFTVLIAAWQAIESKGWLRWALTALIALQVVIIFATATRGAI
                     LGLFAAISVIAVAWAATSGGAGKKAAIGVIGLLLVIAGGLFLIRDSAFVRSEPTLARI
                     VTVFEASELKVRATLWGMAYEGFLERPITGWGQEGYNYVFNKYYKPSLFAQESWFDRA
                     HNTYLDWLVAGGAPALLLFLALLVSAFLSIYRSAASKPEKLILLGILVGYALQAVSSF
                     DNLFSYILLAAILAMAHEKSARPIKQFERAPEMGTSALGTLGAPVILVGTIAVLWTVN
                     VPGIEGSGHLIKAAQQAANPSAALAEYQKAVDSGSFASQEIAEQVLSFGSGVASQATI
                     PMETRQAAVTLALTTMQKEVAKAPNDARIRLMYAGGLRAAGDAAGYEKEMQAALALSP
                     KKQAIIFQKGIASWQAGDKVSAARDFDAGYALDTSFPNAA"
BASE COUNT         4687 a         4756 c         4939 g         4565 t
ORIGIN      
        1 aactcatcac gcacggggaa aacggcatgc tcgttcgttc aggcgattgc gtcgcactct
       61 cgcaagcaat tacggcggta cttgcggacg agccactgcg tgcgcgactg agtgaagcgg
      121 cgaaaacgtc cgcagaacgg ttctcactcg gaacgcttgg aaaggaaact gcagcactca
      181 taaaagaaaa catatgaagg cactcttcgt ctcaaacgat ccatcgatct tcgtttccgg
      241 aagcgctacc cgtgcacgca tgcgcgaata tgccgctgct ataggcactc ttcatatcct
      301 ttctcgtgct ccgaaagggg caaaggaaga agtggaggga aatcttatac tccatcccat
      361 tcatgcacat aagcttctta ctccgtttac tctcgcgcgc cgtgctcgtg cgctcatagc
      421 gcgcgaaggt attgaaatag tttcggcgca ggatccgttt gaacaggggt gggcggcagc
      481 gcgcgcagtg cgtggcacgg cagccaaact acatatccag gtacataccg actttttgac
      541 gccctggttt gtgcgaagcg gcgtgttccg ttcgatgcgt attcatatgc cgtttttaaa
      601 tcgcttccgc gtgcgacttg cgaaaaaagt attgccgcag gcccatggca tccgtgccgt
      661 ttcaaaacgc gttgctgatt ctatcgtggc agcgtatggc gctaccgtgc cggtgcctgt
      721 ggtcattcct atatcagtag ggagtatcgt gccgcaggcg atcccactcc caccacacga
      781 gttctcgttt gcgtttatgt gctctggcag acttgaaccc gagaagcgcg tcgaagatat
      841 tctcatggcg ctcggaaaaa ttgcgcattc ctatccttca gtcggattag tggtggtggg
      901 tgagggaagt gaacggaaga agttagagaa actcaccaag aagctaggac tttcttcgcg
      961 tgtactgttt atgggcgaac aaggaaaaga tgcgtgggga ctcatgaaga gcgctaacgt
     1021 attcgtgcaa gcaagcgcgt atgaaggata cgggcgcacc ttaattgaag cagcgcttgc
     1081 gagagtgcct atcatcacca ctgacgtcgg tatcgtgggt gaagtcctta aaggatacga
     1141 acaggtactc gccaccccgc ccggggatac ggcaaatatc gccgcacaca tgcatgccat
     1201 gatcggcgac catcaggcgc gccttaccct tgctatgaac gccgaaaaag cagcaaaaga
     1261 ccatatagct gcctttgctg accctgccac gcatatcgcc gagaacctct ctgaaacgct
     1321 caaaaagggt gcagataggg catactagtg ataccctcgc gctatgaaca tcgtcactat
     1381 agggggcgga actggcacgt tcgtcgtgct ttccgcgctc cgacgtcttc ccaatctctc
     1441 gctctcggcg atagtctctt ctgcggacga tggcggctcc acgggacgcc tccgggacgc
     1501 ctatggcttc ctcccattag gagacgcacg tcaggcactg gtggcgctcg ccgaagacgg
     1561 gccggtgctc cgtgatctct ttgcgtaccg attcgccaaa ggcgatataa aaggtcacaa
     1621 cctcggaaac cttttcctca ctgcacttac tgatcttctc ggttccgata ctaaagcgct
     1681 tgaagaagcg tcacgcattc tgcgcgtgtc aggacgtgtt atcccagcgt cagaagttcc
     1741 tgccacactt atggcaacgc ttgcagacgg cagcacgctt cgtggtgagc atgtaattga
     1801 tgaaccaaat gcgaatcgtt ccgcaattaa tcaggtatat cttgaaaccg acgtctctgc
     1861 gtctccttca gcgcttgaag caattcgtga agccgacgtt attattcttg gtccgggaga
     1921 cctttatgcg tctaccgtgg caccgcttct tccagcgggg atgcgtgagg ccattgcttc
     1981 ttctaaagga aagcttatat acatcgtgaa cctcttcacg aaagcgggac aaacgggcgg
     2041 catgtcggtc agcgattatc ttcgcgagat tgaacgttat gccgggcgcg cgccggaacg
     2101 tgtgttgata cataagaacg gtggattcga agctaacgtg cttgagcggt atgctgaaga
     2161 aggggagtca cccgtaacgg acgacttagg agaagatgaa cgcgcaagcc gcgcatcagt
     2221 cgcatccgtt catatagtgc cgcccgtgcc agaagatccg gtgccgcgct ctcttgcgcg
     2281 ccatgatccg gaaaaattag ccgccgcact cgcgcccctt atcacactat gaacaagaaa
     2341 ataggattta tcggacaagg atggatcggt aagaactatg ccgatgattt cgagcgacgt
     2401 gggtatgaca tcgtgcgcta cgcgcttgaa gcgccgtaca acgtaaacaa ggaggcgata
     2461 gcagcatgcg acatcgtcat tatcgcggtg ccaacgccca ccactgaagc cgggttcgat
     2521 gcatccattg tgcagggcgt gctaccgctc gtaggagtag ggaagactgc ggtcataaag
     2581 tcgacgctta ttcccggcac taccgaacag cttcaggcgg cctttcctga tcgcatcatc
     2641 atgcactctc cagaattctt gcgtgaggca cacgccgccg aagatgccgc gcacccagac
     2701 cgtaacctca ttggtattcc cgttgatgat gccgtacatc gtgaagcagc agaagccgcg
     2761 cttgccgttc ttcctcctgc tccctacgca aaaatcatga acgcaaaaag cgctgagatc
     2821 ataaagtatg caggtaatgt gtttcttgcg atgaaggtcg tgtacgcaaa catactctat
     2881 gatcttgccg cagcagaagg tgcgccgtat gaagaaataa aagacgcagt gggagcagat
     2941 ccacgcatcg ggtcttcaca cctctcaccg gtagcagcga gcggtcacac cgagaaagca
     3001 gggcgaggcg ccggtggcca ctgcttcatc aaggatcttg aagcgttccg tagactctat
     3061 gacgaattag cgaatgataa agaaggatca gcgcttctag cagcgatggt gcgtaaaaac
     3121 aacgcacttt tgattgcttc cgggaaagac ttggatcttc ttggtggtgt gtacggcccg
     3181 aactatgatg tacttccttg attttgatcg caccctcttt gatacggacg catacaatgc
     3241 gtcgcttgcc gacgaacctg ggtgtgcacc gttcagggaa gatattctca aagtgctctc
     3301 gcagaagcgc gatgataccc tcgttcctcc tgccgagcgt gctcgggtgt ggggtctcgt
     3361 ttcagaagca ataaaatcgg gcgccctttc attcccacca ggctacctcg aacggttcct
     3421 ctacgcagac gttgcagaaa ccttgcgcag ccttggaaac gaggcggtta tcatcaccta
     3481 tggtgaggaa gctcgtcagc gtctgaagat tgaaagtgcg cttgcaaacg cggtacgcgt
     3541 gacggttctt tataccggcg acctttcgaa ggcaaactac ctttcttcat ggcctggata
     3601 ctacggtcaa gaagccgcca tggtagaaga taggactgct gaacttgaag cgctcgctga
     3661 aaagtttcca aagatgaaac tcttcaaaat agagcgcgga gcacgtccgc cccagtccat
     3721 tgaaggcgcg ccatcgtttg aaagcgaagg caggtggaag accattcact cactggctga
     3781 actaccatga agctccttgt ggtgacgcaa gtaatgaacc aagagcatcc ggtgctcggg
     3841 ttcttccatc attgggtaga aacatttgcg ccgcacttcg agcacataca cgttatctgt
     3901 cttgaagagg gagtgcatgc gcttcctcaa aatgtgacgg tgcattcctt ggggaaagag
     3961 aagggagcat cgcgcttcat gcgggtagtg aagttcctgc aactctcatg ggggctttct
     4021 aaggactatg acgcggtgtt cgtgcatatg aacccagagt acctggcgct ctctggcgac
     4081 ttctggcgct tcaccggaaa gaaagtagga ctctggtaca accacgaggt agggagtata
     4141 tggctgaaac ttgcgcagcc tatcgtacac acgctctttt atacgtcacc ctatgcgtat
     4201 ccggcgcgct acaagaatgc acaaaagatg ccggcaggta tcgataccac gctctttact
     4261 tcgggaagtg ccgttcgaag agccgacagc atttatttcc aaggccgcat aagcccagcg
     4321 aagcgcgtac acgttcttct tgaggcgctc cgtatcgtgc gacgtacacg gaaaagcgcg
     4381 acggtaacgc tcgtagggcc tgaagacgcg gcatacggcg cgacgctccg caccgtcttc
     4441 aaagacctca tcgaagctgg cgccgtgacg tttatgggac cgaaacgaaa tgaagaaact
     4501 cctgccctgt atggtgctca cgccgtttcc gtgaatctca ccgcagacgg taactttgat
     4561 aagacggtgc ttgagtcgct tgcatgcgag acaccggcca tcgtttcaag tcgtgcgttt
     4621 gagggactgg tgcccgacga gtggcgcata gcggagaatg atagtgaagc gcttgcagtg
     4681 gcacttcttc gggcactcag cttgtcagca gctcaacttc acacactcgg tgcgaattgt
     4741 cgaaatctag ttgtacaaaa acacagcctc acttctcttg ctgatgagct tgcacaggca
     4801 tacaaccatc gtgcacacta actaatatgg gaatgactat tttaagaaag gctgaacacc
     4861 aggctcttgc ggggtttcca ctctcgggca aggtacttga tcttgggggt gacacgcgtt
     4921 ctgactaccg aaatatgttt tcgggcgact atgcgattac tacagtgaac atgcttcctg
     4981 aagcaaagcc cgatatcctc gctgacctcg aagagccact accaattgag gacgcttcgt
     5041 acgacgctgt acttctcatc aatgtgatgg agcacgtgtt cgagtaccgt gcgctcctgc
     5101 tcgaatgtgc tcgcgttctg cgaccaggcg gtaccatcgt tattatcgtt ccatatcttt
     5161 tcccctacca cgcgtccccg catgattacc atcgatatag cgctgaagcg ctttcgcgcg
     5221 cgctcctaat atctgggttt aaggacgatg tggttcggcc acttggcagc ggtatcattt
     5281 ctgcacgtgt ggtacttact gagcgacttt tgccgggagc cgtacaaagt gtgctcgccc
     5341 taatacttca ccctctcgca ggcatgctcg atgggttgtt ttctagcctt gcacgcctct
     5401 taaggaagaa atacagccca agcgattacg cgctcgggtt cctcgcctcc gcaaagaaag
     5461 ccgtataatc agcagaatat ggaacctgta cggctgtatg tcggacccga ctataaagag
     5521 ccgcaactct acacgtatgt agagcttcta ttcccgttct ggggagtcgc cgcgaaggag
     5581 tcaatgccgt acgtgcgtgc tgcggcaacg cagtatcaat actcaaagaa cgattttgtt
     5641 ctcgttgacg atattaagga tgccgactac gtccttatgc cgtatcacta cgaacgattg
     5701 atggcagtaa accctgagaa ggttgcgaga attgtgcgcg aggcgcaaga agcagggaaa
     5761 ccacttctta ttgatggttc cggtgatatt gaacatgtca ttgatattcc gaatacggtt
     5821 gtgctccgcg tcagtcagta ccgctatgca gtaaaggaga acgagataac ggtaccgttt
     5881 cttgctgagg atttacttga atcattccgt ggcgggattg ttgaactgcg catcaagatg
     5941 caaacacctt ccgttgggtt caccggctgg gcagatattt cttttataac gcggctgaag
     6001 atattcgtga aagaattctc gataactctc ggtgctctgt tcgacgaaaa acgcggcgcg
     6061 gagcataaag gacttttgtt ccgtgctcgt gcactcaaag cgctccagaa gacgaaggga
     6121 atcgtgaaca acttcacaat acgaaagagc tattcggggc acagtgcgac tatacagggt
     6181 gccgtgaagg ataaccggca ggcgttcgtt gatgcgctta taggaagcga ttatgcgtta
     6241 gtggtgcgtg gcgatgcaaa cgcctcagta cgtttttatg aagcactgtc cttaggtcgt
     6301 atcccgttat ttttagatac tgcatgtgtt ctcccacttg aggatcgtat taactatcga
     6361 gacttttgtg ttttcgttga ctggcgagac actgaccaaa taggggagaa ccttgttaag
     6421 ttccatcagt cggtgacatc ggagcgcttt gtagaaatgc agcaccgtgc gcgcgaggcg
     6481 tatcagaact acctgcggat cgatgcattc tcagctcatc ttgccgatat gttacgtgcg
     6541 cggcttacta tacaaaaatg aaacatgccc tcatcgcctt tctaaagcaa gtgaacgcca
     6601 tgccgttccg tttctccatg aagcactatc aggtatttgg tagcgcaacg gaggcgctca
     6661 aggatcagga gctgaactca gcgcaatcat gggatgcgct tcgagagacg cacccattct
     6721 tttctatatc cgagaaccgg gacgagtggc tcgccgcaag tgagcttgcg gtagcaaaag
     6781 acggacagga taccgcgctc gctgcacgtg ctgatgaggt cgtcgcactc atacagcgta
     6841 aaggatttga tcgtgttttc tcaaccgggg ttggaggcgc cgcgcttgaa tatcagataa
     6901 agaagaagat gccgcagctt ccggtagtat gttccgatta tagtgccgag actgtgcgcc
     6961 gcctacaaaa ggttttcact gagagcgacg gtgtcattga gttcgatatt ctcaagggcg
     7021 actggaaagc agtcgagcgt caatatcttg gcacgaatgg actctgcatc atgtatcgtc
     7081 tcgatgcagg attcagcgac gaccaatggc acatcatctt cgatgtgatg caagaagcag
     7141 gtgttacgaa cgtactcgtt attcctaccg gcacgctcac catgctctcc atatataacc
     7201 ggaagaagcg agaacttctc tgggccttga aacatatacc cgtcgtgttt agtggatatt
     7261 tacgtaccaa acatcgattt atatcgcact gggacgaatg gtatgacact gaagaggcga
     7321 cgtttggtgg cctgaaaggc ttccttctga agaagcgagc gcgttagcag ccctgtgcta
     7381 ccatgcctca catcagcata tgaaggagga tgtacagcga aaagcaacca atgaactttg
     7441 ccgagcgctc tcggcatgtc gtatgtgtgc ttccacaaac ctcgtagagt accttgacct
     7501 cgggaaccat ccgccgtcgg atcagttccg gaccgaagaa gagctcacgc tcccggaagt
     7561 gcgctatccg ctccgtgtat tgctgtgcca gaactgcgga ctctcgcagc tctcgcacgt
     7621 cgttgacccc cgagtgctgt accagcacga ctatccgtat gagtcgtcca tgactaaaac
     7681 ggggaagctc cactgggatg ggtttgccga aagcgttata aaacggctcg cgcttcctga
     7741 gaacgcgctg gtcgttgata ttggtagcaa cgtcggcacg ctactcgaat cgttcaaggc
     7801 acagggtatg aaagtcgctg gtattgatcc tgctccgaat atcgtcgaga aagcgaatgc
     7861 cgccggcatt ccaactgtgt gcgacttcta cggtagtgtt gctgcaaata aagtgaaagc
     7921 acagcacgga agtgcggcgg tcattgtagg taccaacgtg tttgcacata tcgatgacct
     7981 cgatgcagtt atggaggcga acaaggagct gttagctgct gacggtgtat tcgcatttga
     8041 atccccacac ttcggccccc tagtcgagca cctcgagtac gacactatct atcacgagca
     8101 cctttcctat ctttcattga aaccagtggt tgcgtttgca aagcgccacg ggctcgaggc
     8161 gttcgcagtt gaagaatcag ccattcatgg cggatcgttt cgcgtatata taggagaagc
     8221 ggggaagcgt cctatagatg cgagtattag caatcttctt atgcgtgagg agcgcgaagg
     8281 tctccatctc cttacgactc ttgaggcgtt cgcacttcgt gtcgcagaaa atcgtgaagc
     8341 acttcgtcgc cttatagaaa aactccttgc tgatggtaag aaaatagcgg ggctttcagc
     8401 gcccgctaaa ggtatgacgc tcctgaacta ttgcgggttc ggcacccgtg agcttgcgtt
     8461 tcttactgag aaatcaacct taaagatagg gcgttactcc ccggggggct atattccgat
     8521 acgggaggac aatgctcttt tagatgagga tgttgactac gcgctccttc ttgcttggaa
     8581 tttcgctccg gaaataatta agaacaccaa ggcattttca gataagggag ggaagtacat
     8641 cattcctata cccaccccga aaatcgtgta atatcgcata tatggaaata acccatacag
     8701 agatagctca cacagatgat cgcggggaga tacgcgacat cctggcgcat gaggatatag
     8761 atgcggttac gtatatcacg tatacgaaag gagctgtgcg cgccaatcat tatcatgaac
     8821 ataccgttca gtgggactac gttatctccg gcaccctcga atcctactca cgcactggat
     8881 tcggtgctcc tatcgagatg aaaattattg gcccaggtga ccttgtgaag catcctatcg
     8941 gcgagcatca tgcactccgt gcgcttgagg actgtgtcac gcttgccctt gctgaagggc
     9001 cgcgtcaggg tgatgagtac gagaacgacg tcgtgcgcct caaggacgac gagaagctta
     9061 tctaatatct atgcacgtac ctatcgggaa gccactcttc ggcgcgatag agcgggaata
     9121 cgtgaatgac gcactggaga agactgaaat ttccggcttc tccggtggct acacctcgaa
     9181 gtttgagaac gcattcgcag cattttgtgg cactaaggaa gcagtaacgg cgagcaacgg
     9241 cacaaccgcg ctccatctgg cgcttgcagc gctcggtata aaggagggcg atgagatcat
     9301 cacacagtcg ttcaccaata tggcgacttt tttcgctgtg ctgtaccaga acgccatacc
     9361 agtaccggta gactcggagc ccgacacact caatatcaac cccgcactta tagaagcgaa
     9421 aataacatcc cgtaccaagg ccatcatggt ggtgcatata tacggtcatc cagtcgacat
     9481 ggacccgatc atggcactcg ccaaaaagca taatcttttc gtcattgagg atgctgcaga
     9541 agcgcatggc gccgaatata aaggaaagcg cgtcgggagt attggcgacg tcggttgctt
     9601 tagtttttat gctaacaaga tcataacgac gggtgagggt ggtatggtca ctacgaatga
     9661 ctcagcgctt gctgagaaaa tgcgttctct taaaggtctt gcgttcggaa aagaaaataa
     9721 attcatgcat gcggatattg gttacaacta ccgcatgacc aacgtacagg cagcattcgg
     9781 cgcagcacag atgacccatg ccgacgagct tgtggcaaag aagcgttcca tcgctggtta
     9841 ctatcttgag catctcgccg gaattcctgg cctcgtactt ccgatagaga aaccatacgc
     9901 caaaaacgtt tactggatgt tcaacattct gcttgatggt cctttgaaag ggaaacgtgc
     9961 ggagttcatg cgcgcacttg ctgagcgcgg tgtcgaaaca cgagaggact ttataccgtt
    10021 taatgaccag gagattttca tcaagcgcgg tctcactcaa acctctgact gtcccgtcgc
    10081 aagcaacgcg ggcgtgaatg gcttttatat tccgagcggg actgacataa acgacgaaga
    10141 acaggcgtat gtcgttgcgc agataaaaga agtagtcaca acgctcacgt cgtaatctat
    10201 gcgtatactc attaccggca ccaccgcaaa ctcgatgccg ccgccctacg ctggggtacc
    10261 gaaactcatt cttgctaccg cacggttatg gcgcgagaaa ggacatgaag tcgcgataac
    10321 ctggcggtat aagcctaaag acgctgacga tttgaatgcg ggtgctacct acttcttcga
    10381 gtatggcgga agcgatcagc ctaataagct tcagaaagtc ggctttctgt tgaagtactt
    10441 cctcaagaat ccttcgctgt atttctcttt gctcgagagc tacaagagta tttgcccgca
    10501 ccactacgat gaggcatacc tctatgcggc ctacggggtt tatctcgacg gaatatacga
    10561 gagttggaaa cctgacgtcg tgctcagtca ggcagctctt ataaagtcat tcatggcggc
    10621 agagctcgcg aaccgtcggc atatacctat tgcattcgat gtgtattctg aggtgcggga
    10681 tttttctatg ggcgtgaacc gcttcctctc ggatgttgaa agaaaacgat actggatggg
    10741 acttcttgga cgcgccgacc tcgtgctcgg tatggataat tgttcggtgg agctgcgcgc
    10801 ctatgcacgc gaaggtacgt taaaagagtt ctgggatacg ggcgactacg agttcttcag
    10861 ccagccaatt cctgaaactc gtacacaact ccgcgagtac ttcagtcttc cttccgacat
    10921 gttcctggtc tcagcggtcg gttccttcga gctccggaaa ggacatgatc atctcattat
    10981 ggcagttgcg cgtctggcga agcagggact caacattggt gcggtcattt gtggaggaca
    11041 ggggaatcgt cagaagtggc gcgacgttgc taaagaagca ggcatcgcag aagatcgtct
    11101 attcttcttc agcaacatct ccgagcttga gctggtgaag ttacatcaca cagttgatgt
    11161 atatacgaac ctctcgaact ctccgcgcat gtgtggatat gatcttgcac ttattgaagc
    11221 tatgtcggca ggtcttccgg tagtggtata tgacaacggt gcgcttccga aagtcatcca
    11281 gggggacagt accaatggct acgtcgttcc gatgaatgat atccctgcag cagctgaagc
    11341 tatgaaaaag ctatacgaaa agacccccga ggagcgtaag gcgatgggga agataagcgc
    11401 tgctttcgcc tctaccatag acctccgaaa gaccacctcc ctaaagctag gctggcttga
    11461 ggaactcgta gtacactctg ggaaatgaaa cgcgcattca tcacaggcat cacagggcag
    11521 gatggcgcat atctcgctaa attcctcgtt gagaaggggt ataaggtgtt tggaggctat
    11581 cgccgctcat cgacacgtaa cttctggcgt cttcactatc tcgatgtgaa gaaagatatt
    11641 gaacttatcc cgatggacct catcgaccag acttctatga tcatggcgct caagaaagcc
    11701 gacccgcacg aggtatacaa ccttgcggcg cagagcttcg ttggtgcctc gtttgatgaa
    11761 gcactttcta ccggagagat atcagggctc ggtgttaccc gcatgctcga ctcggtccgt
    11821 atcgtgaacc cgaagatacg cttttaccaa gcttccactt cagagatgta cggcatcaca
    11881 gcaccacctc aaagcgagac gaccccgttc gccccccaga gtccgtacgc agccgctaag
    11941 ctctacggcc actgggtaac agggaactat cgtacgggat acggtctcca tgcttcttcc
    12001 ggtattcttt ttaatcacga atcaccgata cgtggtatcg actttgttac gaaaaagata
    12061 acagatggtg ttgcgcggat aaagatgaag tacatcaatg agatgtatct cggaaatctt
    12121 gaagccaagc gtgactgggg gtttgctggt gactatgtcg aagcgatgtg gcagatgctc
    12181 cagcaggata agcctgacga ttacgtcatc gcaacagggg aaacccactc ggtacgcgag
    12241 ttctgcgaag cggcattctc ctatgtcggt cttaactata aggactacgt gcgcgtgaaa
    12301 gaagagttca agcgtcctct ggaagtaccg gttcttcttg gtgatccgtc gaaggcgaag
    12361 gcgaaactcg gctggacccc gaaagttggt tttgaagaac tcgttcacat gatggtggat
    12421 gcggatatgg ataagtatca gaatccaact aagcactccg tgcagttcct gaagcatata
    12481 taagtatggg tgaggaagcg ccgtcaggaa gcaacgctga aaaactcgca cgtacaaagt
    12541 atatttcagc gacagcattt acgctcttgc cggagatgcg aacgttgttt cgcggcggta
    12601 agcttacgag cgaatcaggc tggcgtaatg ttgcggagca ttgtttcgca tcggcagtcg
    12661 cagcagacat attcactgac gcactcaacc ttaccgcaga ggaagggcgt gcgctgacgt
    12721 cgacttccgc tgtctctgat tggaataaac gtcttgagaa acgacctggg gatttttcca
    12781 acgaggataa ggcaatcgcg caggaactct tgtcacagat cgctcctgat aagaagcttt
    12841 tagcagcgac acatcctgag ttttttgtac gagcgtatga ggatccgcag aacatcccct
    12901 ttcttgagaa cctgatgctt tacatcgaca acacaacgga tgagagtaac tgggtttcgt
    12961 tccgcgaacg tttaccgaaa gtcatgaacc gttggaagga tcttgatgaa acgtattcac
    13021 cacgtcttgg tggtaagaag tttgttgagt tcgcgataga gcatgatgct tcctttcaag
    13081 aagaactata tgttcgattg aaagctgccc ttgaagcgaa aggaagagaa gcgcctacct
    13141 ctccggatga tataccggca tggcttaatc gcgagcttga taggagggtg aaagaggcta
    13201 caaggagtag cacgtagcga gctttaccta gtctttgctg agaacggtat actgcccgaa
    13261 tatgagaatc tgttacctct ccactgcccg cataccggat gactgggggc atgtcgtcca
    13321 gataatgaag atgtgcgagg cattcgccaa ggctggaaac gacgtcgagc ttgtcgtgcc
    13381 acggcgtgct ggcagacgga acgaaaaccc gtatgcgtac gtaggtgcgg acaacgtgtt
    13441 ccgcataaca aagctaccct gcatagatat atctgcgacc ggagaaggga agttccttta
    13501 ctggctgcgc accttctcgt tcttacttgt cgcgcgcctc tatacgctta taaagaagta
    13561 cgatgttctc tatacgcgcg aaccagcagc atcgctcttt ttcaaagatt tcgtattcga
    13621 agcgcattcg gtctcaaaga gtgtagagag tatctcgaag aaggcatgca aggtagttgc
    13681 tatcacctcg ttcattaagg atgaattggt aacagcaggc gttcgagcac agaacgtgct
    13741 cgtcgcagct gatgcggtgg atcttaacgc atttaagaat gcagagtcga aagagaaagc
    13801 gcgcacacgt ctgggccttc cgcaggataa aaaaatagcg ctctacctag gacgactcga
    13861 ttcgtggaaa ggaaccgatg ttctttatgg tgccgccgac cttcttcctg aggatataca
    13921 gatggtaatc atcggtgtag agcccgcaca gctggacacg cttcgtgcgc agcatccgcg
    13981 tataatcttc ttcgcactga ggccatataa agaagtagct gacaatcagg cagctgcaga
    14041 tgttcttatt cttcctaata ctggaaagaa cgtaatgtct tcccgttaca cttcaccttt
    14101 gaaactgttt tcgtatatgg catctggcat cccaattgtt gcctccgata tatcctctat
    14161 acgcgagatt ttgaatgaat ctaacgccat gttcgttaca cccgatgatt cgatggcact
    14221 agcacaaggt attaaaaaga cactcacaga tctttcagta tcagaaaagc gtgccgagaa
    14281 agcacgtgat gatgtcgagc tccatacttg ggatgcgcgc gcctctgcca ttctttcctt
    14341 tctcagtata tgaaaaaact aactcgagga agtatcgatg ccttcttgaa taagtacgca
    14401 agcgacagaa agacgcttga tataggggca gcgtcgggcg atcaccctat gttttcaaac
    14461 agtaccgcac ttgatattga cccagcacga aatccagacg tggtagggga tgctcataac
    14521 ttgccgtttc ccgacgcctc tttcgacatc gtcgtctgct ccgaggtttt tgagcatctc
    14581 acgaatccgc ctaaggcggc agcagagatg catcgcgtac tagtgccagg aggcatgctt
    14641 atactcacga ctcggtttct cttccccgtg catgacgctc caactgacta tttccgctat
    14701 accccctacg gactccgtca tatttttagg gaatgggaga ttatcgagga aaggtgcgag
    14761 tctgattcat tttcaacaat agctgtgctg ttgcagcgca tcatcttcca aagcgagttg
    14821 aagggaggaa aagtgacaaa aggaatattg tggctctttg tacttttctt tagtcagctc
    14881 gattttctta cgatgaaacg atatggagag ataggacgga aagagtccat tccctcactt
    14941 ctctcctctg gcgtatatat tgtctgccgt aagtcggaat agtgatatcc ttgcgttatg
    15001 aatagagaga cgatccaaga ggatgaatat gaattcccat accatcacat accaacgcac
    15061 gcaccctttg cgcaatcgcg tctcttattt tggggatacg aatacgaagc gtatctcgaa
    15121 gagatactcg ccgtgttaga aggaatgagt ttcaaatcac tcatagatat aggatgcgga
    15181 gatgggcgtc tcttgtcgga acttgtgaaa agctctaatg cccgtctagt aggcactgac
    15241 ttttcggaga aagcactggc gttcgcccgg gcgttctctg cgtcggtgga gtttcagcgc
    15301 gatattccag ataaagaaac atttgatgtg cttaccttaa tagaggtact agagcatatc
    15361 cctcctgaag aaatccctac gtttcttaat acacttcgtg gagcgctggt acctggaggg
    15421 aagggggtta ttacagtgcc gagtgtcgct ttgccggtaa accgtaagca ttatcaacat
    15481 ttctccgcag aatctcttac agaaacccta tctccatatt ttgttatcga ctcgattaca
    15541 catctcaata cagtctcgtt aggctcgaag ataatcgcgc gattgctctc aaatcgattt
    15601 tttattctca atcactctgg tatgcgcgaa tggctttatg gaatatataa gaagcaccac
    15661 cttcgtgctc atccgggtaa gggtgctcgc cttatggcga ttgttacacc acgagcttga
    15721 gcttccttat gcgagctgaa tacgagtagg tgaggatgat acctagtact aagaaaaata
    15781 tacgccctat aatccgcgct atcactgcgc caagaagtcc catcgtagaa atcagtataa
    15841 gaagtattgc aatctggaac aggggccccg ccacattata aatatagagt tcgcgtttgg
    15901 cagcatgtgc ctcaagaagt gctaggggga ccacagcacc gactggtatt aaagtaactg
    15961 catacaagac cgaataaaag attgcttcgc tgtatgccgg gaaaagtata tggaatacga
    16021 agggcgcgat aaaggcataa atcatagcag caacggctgc tatgactgaa agtccaagga
    16081 gtcggtatac gagtgtgctt tttacttcct caagaggtcg ttttacaaac ttgggcaacg
    16141 ccaatgtcgc gacactatta aagagtcctt ttatctgatc aggtattgcg gttgcgaacg
    16201 catacacggc aagctccact gcgccaagaa aatggaatac aagaatccta tccacctgcg
    16261 tggagaccgt attcagaata ttcatcagac taaaatgtgc tgaaagtgtt ccaaaccctt
    16321 tcgattgagt tttgagcgat actgcttgtt tcttgactac gacataggaa acaacgaacc
    16381 cggtaaggac actcgttccc aaatagatga gaaggaatat aacaggatca tcaatgaaga
    16441 acatgcttgc taatagcgcg agcgcaggga ctaggttcag tactatgccg cctatcgcgt
    16501 tctctcgaaa tgcctgcttg ccttcaagat aggaaccgta caagtacgct ccttgcatga
    16561 aggggccgat aatagcgatg acgagtgcgc ctgctgcaag tactgtgttt tcatttagat
    16621 aatagtagat gctagttccg agtcctatga tcgccatcgg aatgctccat agcaaagaga
    16681 gacggaggga tcgagcgtag gcgccgcttt ctccacgcgc taccgcgcgt gcaaggactg
    16741 tagaaatacc agtgaacgag aaggcagtca gtatccagaa tatggaaaga aggaagcgat
    16801 agttcccata gacgtctttt ggaacgaagc gggcaaaggc cacggatacc cccaacgcaa
    16861 taagacctat acctacctgg cccacggcga gccagaagcc gtttccggcc aggtatacca
    16921 tgtcggtctt cgtataacgt tcgctccatt tgaggaggct gaatactcgt tcccgtagtg
    16981 aggaaagcat ttctcctatc ataccgcctt actcctatag ggcgagggtt cattgagaga
    17041 ggggtggcga aaccgtattt gccccaagaa cactgatttt cacccagtag aacgccattg
    17101 aatcgctgca atggtatagt ctcccctata tggcacttac caccaccgct cgttggacga
    17161 ttctcggcgt tttgttcgtt atcccgttcc tgcctctttt tgtggcgaac ggaatgtttt
    17221 tcccctttat ttcaagcaag gggtttgcct tccgtatcct tattgagata gcagcaggag
    17281 cctggctgct cctcatgctg gcggataagc ggtaccgccc ccagttctcg tggccgctag
    17341 ctatatatgg agcattcgtc ctctggatgt tcatcgctga cctgtttgca gcaaatcccc
    17401 ataaggcgtt ctggagtaac tacgaacgta tggatggatg ggtgacgatg atacacgtct
    17461 tcctcttctt cctcgttgct ggttccttcc tttccgtaga gaagctttgg aagcgttggt
    17521 ggcttaccct gcttggcgcc tcagctctcg tttctgtata tggtctctgg cagatatttg
    17581 gtctcgcgca aattcatcag ggcggcgtgc gtgctgacgc gtccttcggg aacgccatct
    17641 atcttgccgt gtacctcatg ttcaccgtgc tcatagctgc atggcaggca atagagtcga
    17701 agggctggtt acgctgggca ctcacagcgc ttatcgcact tcaagtggtg atcattttcg
    17761 ctaccgcaac gcgcggcgcg atactcggcc tcttcgccgc tattagtgtc atcgcagtag
    17821 catgggctgc aaccagcggt ggagcaggga agaaagcggc tatcggggtt attggactac
    17881 tcctcgttat tgcgggagga ctcttcttga tacgcgacag cgcctttgtc cggagtgagc
    17941 caacgctcgc acgcatcgtc acggtattcg aggcaagcga gcttaaggtg cgtgccacac
    18001 tttggggaat ggcgtacgaa ggattcctcg agcgcccgat aaccggatgg ggtcaggagg
    18061 gatacaacta tgtattcaac aaatactaca aaccttcgct attcgctcag gagtcgtggt
    18121 ttgaccgtgc gcataacacg tatcttgact ggctcgttgc cggtggtgcg cctgcactcc
    18181 ttctcttctt agcgcttctc gtgtctgcat tcctctccat atatcggagt gctgcctcaa
    18241 aaccagagaa acttattctc ctaggcatac tggtggggta cgcacttcaa gcagtgtcat
    18301 cgttcgataa cctctttagt tacatcctgc ttgccgctat tcttgcgatg gcgcacgaaa
    18361 agagtgcacg acccatcaaa cagttcgaac gcgcaccgga gatgggtacg tcagcgctcg
    18421 gtactctcgg tgctccggtc atccttgtcg gtaccattgc ggttctctgg accgtaaacg
    18481 ttcccggaat tgaaggatca ggacacctta taaaggccgc acagcaggcc gctaaccctt
    18541 cagcagcact cgctgagtac cagaaggcgg tagatagcgg ttcatttgct tcgcaagaaa
    18601 ttgccgagca ggtactttca tttggaagtg gcgtggcttc tcaagcaacc ataccgatgg
    18661 aaacgcgcca ggcggcagtg acgcttgcgc tcaccacgat gcagaaggag gtagcgaagg
    18721 cacccaacga tgcccgcata cgccttatgt atgccggcgg tctgcgcgct gcaggggacg
    18781 cagcaggcta tgaaaaagag atgcaagcag cgcttgcgct ctctccgaaa aagcaggcaa
    18841 ttatcttcca gaagggtatt gcgtcatggc aggcgggaga caaggtgagc gctgcgcgtg
    18901 attttgatgc ggggtatgcg cttgatacct ccttcccgaa cgctgct
//
LOCUS       DAORIN010000003        17056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_562_length_17056_cov_5.659432, whole genome shotgun sequence.
ACCESSION   DAORIN010000003 DAORIN010000000
VERSION     DAORIN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17056
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_17056_cov_5.659432"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /gene="serS"
                     /locus_tag="PK109_00275"
     CDS             complement(<1..1246)
                     /gene="serS"
                     /locus_tag="PK109_00275"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRH24016.1"
                     /translation="MLDIRFIRENADVVKEGARKKNVAIDIDRLLVVDEERKKLLQAI
                     EEKRAEQNRASKTIALAKGEEKEKLLEAMRHLKEGMGEMQTAFEALDIEWTKLMLAVP
                     NIPSPDTPEGPDESGNKVVRSWGEPTKFSFEPKAHWDLGKELDIIDTEKAAEVSGARF
                     AYLKGDLALLQFALLHHTMNVLTSPEELQKAAREAGVEVPTTPFVPVVAPILMRAEVM
                     GRMARLHPIDERYYLEKDGQVLIGSAEHTLGPLHMDEIIDESRLPLRYVGYSPSFRRE
                     AGAAGKDTRGILRQHQFDKIEMESFVRPEDGYREQDFFVAIQEYLMRSLKLPYQVVAI
                     CTGDMGKPDHRQFDIETWMPGQNVYRETHTSDYMGGYQARRLNTRVKDAQGEMTHIHM
                     NDATAFAMGRTLIAIMENYQQED"
     gene            1547..2200
                     /locus_tag="PK109_00280"
     CDS             1547..2200
                     /locus_tag="PK109_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRH24017.1"
                     /translation="MESRILTKADMPAHLLEIPQPPEKLWARGSWGPKRNKMLAVVGS
                     RDLSPYGREACQSLIKGLAGYPISIISGLALGTDACAHDAALAAGLHCIAVPGSGLGD
                     RIISPRTNAALAANILSAGGLQLSEHPDDYTPRPYDFPSRNRIMVGLSQAVLLIEAGN
                     RSGTLVTARLTHEYNRELLCVPHRITDPHGFGTNLFVRLGATLVAEPAHILEVLGFN"
     gene            2552..4975
                     /gene="topA"
                     /locus_tag="PK109_00285"
     CDS             2552..4975
                     /gene="topA"
                     /locus_tag="PK109_00285"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRH24018.1"
                     /translation="MKKTLLIVESPAKARTIQQYLGTEYEVLASVGHVRDLPKTSKDA
                     VDIEAGFVPRYVISPDKREVIERIHKAADRAGDVLLATDPDREGEAIAWHIKEAVGLK
                     KPKRVVFHEITKDAIKEAIEHPRAIDEDLRKAQEARRVLDRLVGYNLSGLIWKKVRYG
                     LSAGRVQSPALRILAEREREIRAFIPETYYVLDARFKSSHGEFSATCVEEPKTAQDAA
                     RIVELGEKASWVIGNVIERAEERAPRPPFTTSTLQQTASTRLGFAPSRTMRAAQKLYE
                     AGHITYMRTDSVNLAKEAVGKLAAAAEKQFGKEYVQVRTYQTKSKNAQEAHEAVRPTD
                     PAKAKAGATGDEQALYELIRTRAMASQMASAQLLRTKVEAKADADIPLFTANGSRVVF
                     PGWLALDTRARGEDVELPKVSAGDPLTVITLGSLEKQTEPPNRYTEAGLIKELEKRGI
                     GRPSTYASIMKTIQDRGYADKDGRTLKPTATGMVVSGWLEEHFAEYISDSFTAEMEDE
                     LDEIARGEREYVKTMKDFYGPFKKAVDGKEDLPKATSLGEVPKEFTCPKCGSTMEFKL
                     GRGGVFMSCTRYPDCDGARQEDGHEIKADEPLGIHPETGRPIFMRTGRFGPYVEMPDA
                     PTEVTPEPEVVPPSASALTKKGTPRKVRATKPKKPKKPAFSGKRASIPPGKEVSSVTL
                     EDAVKYLSLPRDVGVNPATGHMVVANTGRFGPYVAHDGEFRSIKKADPYTITLDEALV
                     LINTPKQPPKGAVIVREVGKHPKTGKALVLYKSKQGLFLKKGLRRIYLPDSVNEDEMT
                     PGEAASYLA"
     gene            5347..6975
                     /locus_tag="PK109_00290"
     CDS             5347..6975
                     /locus_tag="PK109_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRH24019.1"
                     /translation="MTTVKEILAEMKAPTPEDSALVQKAYDFAEKAHKNEKRYSGEPY
                     FIHPAATAKVLASLGMDAPTIAAGLLHDAIEDAKVTEEEVTREFGAEVLFLVEGVTKL
                     GKHKYRGAERHAESLRRLLVATSADVRVLIIKLADRYHNMTTIEHVPKEKQRRIALET
                     LEIYAPLADRLGMGRMKRDLEDLAFPVIDPDAYTHAVEVRKLKTKESESGLVRVQKEL
                     QRELARKGLRNFRTEIRMKGYWSLHQKLKRKGDDIATIHDIAALRVVVQTVDDCYTAL
                     GVIHARFRPLPGEFKDYIAFPKPNGYQSLHTTVLTSDAGVVEMQVRTDDMHRRAQFGI
                     ASHMSYKQLGKAAAKGAFEVLSFSWIRDLVPTLLNFSKKPNVTPVVLSDKEQKKATRE
                     EKPYWLSELADVHTTLSESSEFVDRLKEDFFSHRVFLFTPKGDVVDLPMHSTPIDFAY
                     AIHSDLGDHMIGAKVNAKMVSFDTPLRNGDIVEILRKESAHPTAKWLDMAKTSVARKH
                     IRSALEVAERGTTTPIKPDTRLLRKRANKKKPKS"
     gene            complement(6972..7937)
                     /locus_tag="PK109_00295"
     CDS             complement(6972..7937)
                     /locus_tag="PK109_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRH24020.1"
                     /translation="MHDKLVNIMQEPTKYDSIYWVEVDKISPNPYQPRKEFNEDALKS
                     LAESIRQYGLLQPLTVTRSEVSRPDGGLESRYELIAGERRLRASKLIGLKSVPVVIRG
                     EESNKVKLELAIIENLQREDLNAIDRAKALAQLVEEFGLSHSEVGAKVGRSREYVSNS
                     VRLLALPDAIQQAVVDKDISEGHARALLMLNDRPEERDTLFKEILIKKTSVRMVEQLA
                     RRIATDKIRKHDKTPEMMEIEKSLTETLGTRVVIENRPNGGRVLIEFFSPEDLSNIVS
                     ALAVEASEKATEVLDETLPPVDAATTEEVAEKSDEELYSVKNFSL"
     gene            7989..8073
                     /locus_tag="PK109_00300"
     tRNA            7989..8073
                     /locus_tag="PK109_00300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8023..8025,aa:Leu,seq:caa)
     gene            8151..10022
                     /locus_tag="PK109_00305"
     CDS             8151..10022
                     /locus_tag="PK109_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24021.1"
                     /translation="MAYEGAPVNPVPVEPDKEHSASLTKQMKEALEKLVKQHGETSKQ
                     DALEEEGEDREPPPEIPSYLRSHEDTSFEARFLRRWVEDSVVRYVSTWKFEDFAKFMK
                     DKGRMARGVYALGLPTSNVGPASSRDSKPQKLAAKRATMREGWRNTVQHEFYVAFRTL
                     MWELVRGRYHVVHEVTPGKVRVDNTHDDSHVLSEILHAMLRAEEPVQGETGMTREQKL
                     EIVDMLVHSVQEEVAVLEESLAPGWEALAPADESDQFNIGFLDRARIGKLSVPERAER
                     MYQMIHLAKELNAVVDTTLDGAGRPLINNRIRLPENISVSRSDFAVIMNEFGFDLGFM
                     SGMARYVGREFSLQKSQGVAKLDSEGIKDLAEHKKAFLKAAEALPDIHILDSLNRLAQ
                     TGLLPLYNEHNEITALLKARKDVDPAYLSELAQQSLNISPAVRQERSSPKDALDDAGA
                     LATKWETIASESGPAAATAKKNATAELKKYFAIHAKNDSIKLLAESLELFKSGPDIGK
                     IVAPIIEKRLASLASINLRSALIETRELRKMGYDALITDKAIQALGLRAKDLVEEMLV
                     RTSSQDTYLFFRELLASGLGEFVLTEKAASLWTERILAHDIEFSRTEVLKEELQLGS"
     gene            complement(10026..10631)
                     /locus_tag="PK109_00310"
     CDS             complement(10026..10631)
                     /locus_tag="PK109_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRH24022.1"
                     /translation="MKEYVSSVKVGETVPDYEFEAYYKDDTKAMKFSDFRGKWLVLMF
                     YPADFTFVCPTELEEMAKLYLKFKEINAEVVSFSTDTVFTHKAWHDTSAAIKQIEFPM
                     GADPSGKISYAFGTLIEGDGTELVEDEGLSMRGTFIIDPAGTLRSMEMTHNSIGRKAA
                     ETLRKIQAAQYVEAHPGNVCPASWEPGDDTLEPGMDLVGKI"
     gene            10744..11586
                     /gene="miaA"
                     /locus_tag="PK109_00315"
     CDS             10744..11586
                     /gene="miaA"
                     /locus_tag="PK109_00315"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRH24023.1"
                     /translation="MSGKTPIVCIIGPTASGKSGRAIALAKERNGEIISVDSRQVYRM
                     LDIGTEKISKEEMQGVPHHLIDIRDPKESYSAGDFAKDATHLIEDITKQGKLPILAGG
                     SHFYFDALINGIPGETPMDPVLRESLEKMSDEELFEKIKEGDPSRAETIDPKNRRRLV
                     RALEIITLHGKVPERSKASLYDVEWIVLLPEREELRARIEARLKETLEKGLIDEVRRV
                     RDYVGDERLNELGLEYRIVGEYLRGERDEETLLHALTAKLFQYARRQEAWIKKLESSS
                     IRLR"
     gene            complement(11570..12649)
                     /locus_tag="PK109_00320"
     CDS             complement(11570..12649)
                     /locus_tag="PK109_00320"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRH24024.1"
                     /translation="MRFIKVLLPVLVFGILALTPHQASAATATFFGPIIPPECHCETT
                     APDFGCVLQVIENLMNFAISIGVVIFVLVAAYAGLLWMFSPMNAHNREQGRTILLNAV
                     VGLLITLCAWLIVDFVMKTLYNEGSYGPWNAILADGKECLVAITPPAGSTPSDGGLTT
                     GAGTGPNDDKDADGKPNSTDDDDDNDLIKDQVDNCPFVANQNQADADNDGTGDACETA
                     TGAGLVPITAFNCKNANSCQTTAGRNSSLNQMASALGSAASQYQITETGVNTSVTHAS
                     GSNNLDMSCFGTCSSAQVLAAIAAWPGTVKYETNSQSDYNAKRNAGVPAANMLLLTGR
                     FVNGQCSTGTGHCITAPHMSLNGGG"
     gene            12725..12795
                     /locus_tag="PK109_00325"
     tRNA            12725..12795
                     /locus_tag="PK109_00325"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12757..12759,aa:Trp,seq:cca)
     gene            12866..13693
                     /locus_tag="PK109_00330"
     CDS             12866..13693
                     /locus_tag="PK109_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24025.1"
                     /translation="MKFLERIGLSKKSEKVEEATGPEVVITEQEKIELVRDLSKILES
                     LRPDIESGAIGSVVGIDRSGRPVALALKHAISERYKTLGHKPIETRFFNGLRLGIKPG
                     HDIRKINRVDKNLLEALETHPLDKTRKVIIADDFIGWMETMPSFIRVFTNAGYQVEVA
                     ALGSRPSHLRGQKDATFKFRDSYMVAVGEPGRQHTEHKLETYEAPLHQGDSLDGFKKG
                     GKLSGVQTDWSGGAHALKQPTTPGLGATIRQGRQDIIEIAEAALAAYQQSKDDTRSS"
     gene            14387..14893
                     /locus_tag="PK109_00335"
     CDS             14387..14893
                     /locus_tag="PK109_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRH24026.1"
                     /translation="MPKVIIVDENDTPIGLKERADFLPGDIHRVTALWLTNSQGEILL
                     AQRAFTKKHAPGMWGPAVSGTVDEGETYESNVIKETKEEIGLSISLDELKKGPKYLAG
                     EGTENGRFVQWFSAVRDVAIDELTLPPDEVIAARWITLADLKREVLEAPDRFVSAMSR
                     TPPIYEAF"
     gene            complement(14924..15079)
                     /gene="rpmG"
                     /locus_tag="PK109_00340"
     CDS             complement(14924..15079)
                     /gene="rpmG"
                     /locus_tag="PK109_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRH24027.1"
                     /translation="MSQDTLIKLKSTKSDTVYYTRKNKKGVERKIELKKFDKRTGKRE
                     VFKEAKK"
     gene            complement(15117..>17056)
                     /locus_tag="PK109_00345"
     CDS             complement(15117..>17056)
                     /locus_tag="PK109_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRH24028.1"
                     /translation="KYRGEFEERMKTVMKEVERAEGKVILFVDELHTLVGAGAAEGAM
                     DASNMLKPALARGEIRIIGATTLKEYQQHIEKDTALTRRFQPVYVNEPSVEDAVAILR
                     GLRDKYELFHGVRITDGAIVSAVELSTRYISDRFLPDKAIDLIDEAASGLRIALENKP
                     PALEETDRKIRRLTIEREALTKDEETSSTAGEHVKEIRERIKAIDTEIADLKETTSEL
                     ELKWKNEKEVLEVIRSIKNELDKLRVQAENAEAIADLGTAAEIRYGKIPTLQKELEVR
                     MKRLKTLQKSRRVLHEEVTESEIAGVVSRWTGIPVSRMIEEEAAKLARMEEALTGAVV
                     GQDKAVKIIADAVKRSRVGIADPNRPIGSFMFLGPTGVGKTELSRRLAEFMFNDKDAL
                     VRVDMSEFMEKHSVSKLIGAPPGYVGYEEAGSLTERIRHRPYAVVLFDEIEKAHSEVF
                     NILLQVLDSGHLTDGKGRKVNFKNTIIVLTSNVGAEYMDRMAGIGFSQSGNDAAAQSE
                     QMKEKVMSSLKDNFRPEFLNRLDDIVMFDVLGKETLTKIVEHQVEEVTKRLLGKRITL
                     TLSPEVKAWLAEGGYNPQYGARPLKRAIQDKILTPIATLMVGQGVMEGGSINVTMKAG
                     EPHFEVKKSVSRVRKAKAEAV"
BASE COUNT         4269 a         4461 c         4201 g         4125 t
ORIGIN      
        1 catcttcttg ctgatagttc tccataatcg caataagggt gcgtcccatg gcaaatgcgg
       61 tcgcatcatt catatggatg tgcgtcatct ccccctgtgc atcctttacg cgcgtattaa
      121 gtcgccgtgc ctgataccca cccatgtagt ccgacgtatg cgtctcacgg tacacattct
      181 gtcctggcat ccacgtctca atatcaaatt gccggtgatc gggcttaccc atgtcccccg
      241 tacaaattgc gacgacctga tacggaagct tgaggcttcg catgaggtac tcctgaatcg
      301 cgacaaagaa atcctgttca cgatacccgt cttcaggacg aacaaatgat tccatctcaa
      361 tcttgtcgaa ctgatgctga cgcaagatac cgcgcgtatc ttttcctgcg gcacccgctt
      421 cacgacggaa tgatggtgag tagcccacgt aacgaagcgg aagtctcgac tcatcgataa
      481 tctcatccat atgaagcgga ccaagcgtgt gttcagcgct tccaattaat acctgtccat
      541 ccttttcaag atagtagcgc tcatcaatag gatggagacg tgccatgcgg cccattactt
      601 ccgcgcgcat aagaatagga gcaaccaccg gaacaaatgg tgtagttggg acctctactc
      661 ctgcctcgcg tgccgccttc tgtaactctt caggcgaagt aagtacattc atcgtgtgat
      721 gaagaagtgc aaactgaaga agagcgaggt cgcctttcaa gtatgcaaag cgggctccgg
      781 acacctctgc tgccttttct gtatcaatga tatcgagctc tttcccaaga tcccagtgtg
      841 ccttcggttc aaaagagaat ttagttggct caccccatga gcgaactact ttgttcccgg
      901 actcatcagg gccttctgga gtatcaggag aagggatgtt cggaacggcg agcatgagct
      961 tcgtccattc aatatcaagc gcttcaaatg ccgtttgcat ctcccccatc ccttccttga
     1021 gatgacgcat ggcttcgaga agtttctctt tctcttcacc ttttgcgagc gcaatagttt
     1081 tggacgcacg attctgttca gcgcgctttt cctctatcgc ctggagtaac ttcttacgtt
     1141 cctcgtcaac aacaagaagc ctatcgatat caatagcgac attcttttta cgtgcgcctt
     1201 cctttacgac gtcggcgttc tctctgatga accgtatatc caacatgatg tgctgattgt
     1261 acgccatagc gcgccttccc gaaagaagga catgtggtat agtcctgtcg tcgagtaatg
     1321 gaggttcctt atgagtgtat ggggtgagca cgaacggtgt gaggattcaa aacttccgcc
     1381 caccaaccca gacaacaccc ttcccacatc ggcggttaaa cgtttgatgt acggcatgga
     1441 gggacacgaa gacccgccta tcgaaactga gtgaagtgcg agcacttcac ttctagggcg
     1501 cgcaagaacc attcttgcgc gcccgactct attgcatact agaggtatgg aaagccgcat
     1561 ccttaccaaa gccgatatgc cggcacacct ccttgagatt ccgcagccac cggagaagct
     1621 ctgggcacgc gggagttggg gaccaaaaag aaataagatg cttgcggtgg tggggtcacg
     1681 cgatctttcg ccctatggac gcgaggcatg ccagtcgctt attaaaggac tggctgggta
     1741 ccccatatcc atcatttcag gtctagcact tgggacggat gcgtgtgccc atgacgccgc
     1801 acttgccgca ggccttcatt gcatcgcagt cccagggagc ggattgggcg acagaataat
     1861 ttctccgcgc actaatgctg cccttgcagc aaacatactt tccgccggcg gccttcagct
     1921 ctcagaacat ccggacgact acaccccgcg cccctatgac ttcccgtccc gtaaccggat
     1981 catggtcgga ctttcccagg ccgtcctttt aatagaggcc gggaatcgct ctggtacgct
     2041 cgtgaccgcc cgccttaccc acgaatataa tcgtgagctt ttatgcgtcc cgcatcgcat
     2101 tactgaccct catggctttg gtactaatct ctttgtcagg cttggcgcca ccctcgtagc
     2161 ggagcctgct catattcttg aggtgctcgg tttcaattga cgccctcctt atttaggaat
     2221 acaactctag caagggtatg taaccctgga gaaaaaatga tggtctacat caccgacctc
     2281 tccccatcac cagcgctgat gagacgattg gcatacgcat ttcccggcgt cgatatcgaa
     2341 aacacagaaa ttggaatact tcttaacaaa gaagatccgg atgaattcca acgagcagtg
     2401 agagccgaaa tcgaacagtt ggtaatcgaa acgcaaggcc aataacgcag gcagaagcct
     2461 ggtgaattgc gacggaagaa ctccgtcgct tttcattttc tctccacact cgatttgaca
     2521 atatataagg tatcgcgtta tccctaagga aatgaagaag acactattaa tagtggagtc
     2581 acccgcaaaa gcgcggacta ttcagcaata ccttggtacg gaatacgaag tactcgcttc
     2641 tgtaggacac gtacgcgacc ttccaaagac gagcaaagac gccgttgata ttgaagcagg
     2701 gttcgtccct cgctatgtta tttctcctga caaacgggag gttattgaac gtattcataa
     2761 agcagcggac cgcgcaggcg acgtacttct tgcgaccgac cctgaccgtg aaggagaggc
     2821 tatcgcatgg catataaagg aggcggtagg actcaaaaaa cccaagcgtg tggtattcca
     2881 cgaaatcacc aaggacgcta tcaaggaggc cattgaacat ccccgcgcta ttgatgagga
     2941 tcttcgaaaa gcacaggaag cacgtcgcgt attagaccgc ctcgtagggt ataacctttc
     3001 gggtcttatc tggaagaagg tgcggtatgg cttgtccgct ggacgcgtac aaagcccagc
     3061 gcttcgcatc cttgctgagc gcgagcgtga gatccgagcc ttcattcctg aaacctatta
     3121 cgtactcgat gcgcgtttta aatcctctca tggagaattc tccgctacct gcgttgaaga
     3181 accaaagacc gctcaagatg cagcgagaat cgtcgagctc ggtgagaaag catcgtgggt
     3241 cataggaaac gtcatagagc gtgctgaaga gcgtgcccct cgtccgccgt tcactacctc
     3301 tacacttcag cagacggcgt ccactcgtct cggttttgca ccgtctcgca cgatgcgcgc
     3361 agcacaaaaa ctgtacgaag caggacacat tacctacatg cgtaccgact cagtgaacct
     3421 tgctaaagaa gcggtaggaa aactcgctgc cgctgcagaa aaacaattcg gcaaggaata
     3481 cgttcaagta cgcacctacc aaacgaaatc caagaacgcg caggaggcgc acgaagccgt
     3541 gcgtcccacg gacccagcaa aagcgaaagc gggtgctacc ggcgacgaac aggcgcttta
     3601 cgagcttatt cgcacgcgtg caatggcgtc tcagatggca agcgcacaac tccttcgcac
     3661 caaggtagaa gcgaaagcag atgctgacat tcccctcttc accgcaaacg gctctcgagt
     3721 cgtgttcccg ggttggcttg cgcttgatac gcgcgcacgt ggagaagacg ttgagctccc
     3781 taaagtttcg gcaggtgacc cgctcacagt catcacgctt ggctccttag aaaagcaaac
     3841 ggaacctccg aaccgataca ccgaagcagg gctcatcaag gaactcgaga agcgcggcat
     3901 tggccgcccg tcgacctacg cctccattat gaaaaccatt caggaccgcg gctacgcgga
     3961 caaggatggc cgtaccctta aacctaccgc tacgggcatg gtggtgtccg gatggctcga
     4021 agagcacttc gccgagtaca tcagtgattc ctttaccgca gagatggaag atgagcttga
     4081 tgagatagcg cgcggtgaac gcgaatacgt aaagaccatg aaggatttct atggtccctt
     4141 taaaaaagca gttgatggaa aagaagatct tccaaaagcg acgtcacttg gtgaggtacc
     4201 gaaagaattc acctgtccaa aatgtggcag caccatggaa ttcaagctcg ggcgtggcgg
     4261 tgtgttcatg agttgtacgc gctaccctga ctgtgatggt gcgcgccagg aagatgggca
     4321 tgaaattaaa gcggatgagc cgcttggcat acatccagaa accggacgcc ctattttcat
     4381 gcgtaccgga cgctttggtc cgtatgttga gatgccagac gctccaactg aagttacccc
     4441 tgaacctgaa gtggtgccgc cttctgcttc cgcactcacc aagaaaggga cgccacgcaa
     4501 agtgcgtgcc acaaagccga agaaaccaaa gaagccagca ttctccggaa agcgcgccag
     4561 cattcctcca gggaaagaag tttcctccgt aacgcttgag gacgcagtga agtatctttc
     4621 ccttccacgc gacgttgggg taaatcccgc taccggacac atggtggttg ccaacaccgg
     4681 acgtttcggt ccctatgtgg cgcacgacgg cgagttccgc tcgattaaga aagctgatcc
     4741 gtacaccatc actctagacg aggcgcttgt ccttattaat acgccaaagc agccacccaa
     4801 aggcgccgtg attgttcgtg aagtgggtaa acacccgaag accggtaagg cgttggttct
     4861 gtataaatca aagcagggac tcttcttgaa gaaaggcctg cgccgtatct atcttcctga
     4921 ttcggtgaac gaagacgaga tgactccagg agaagccgct agctatttag catgagataa
     4981 ggccgctgcg tcatgcagcg gccttttcat ttacgagact cttcgcatta ctacccaacg
     5041 ttagtgggtg tcagcgactc gaagcccagg tttggatttt cctggaactt gagttcaagt
     5101 ttctcgaaat aaggttttcg gtcagtagcc ttattgtaaa tatcgagcac tcgattagct
     5161 tcatcagcta atgcaactga atttgttctt gaatcttcgg gggatgcagt cgtttcggtt
     5221 cccgtagtca ttgaacatct cctcaacatg ccacgcgcat gcctgttgtt gcaccatacc
     5281 gtacgacatc ttcgtccgtc aatcgttatt tggtaagaac cgcctctatc tagtactcta
     5341 agaagcgtga ctaccgtcaa ggaaatcctc gcggagatga aggcaccgac gcctgaggat
     5401 agtgcgttgg tacaaaaggc gtatgacttc gccgagaagg cccataagaa cgagaaacgc
     5461 tactcggggg aaccctattt catccatcct gccgctaccg caaaagtgct tgcgagcctc
     5521 ggcatggatg cgccaaccat tgctgctggt cttcttcatg atgctattga agacgcgaaa
     5581 gtaaccgaag aagaagtgac gcgtgaattt ggtgcagaag tcctcttcct tgtagagggg
     5641 gtgacaaagc ttggaaagca taagtatcgg ggtgctgagc gccacgctga atcgctccgt
     5701 cgccttttgg tggcaacgtc cgctgacgta cgcgtcctca tcatcaagct tgctgaccgg
     5761 tatcacaaca tgacgaccat agagcatgtg ccaaaagaaa agcagcgtcg cattgcactt
     5821 gagacgctcg agatatatgc acctttggct gaccgccttg gtatggggcg catgaagcga
     5881 gatctcgaag atcttgcctt ccctgtcatt gaccctgacg cctatacgca cgcggttgaa
     5941 gtgcgtaagc tgaagaccaa agaatccgag tcagggctcg ttcgggtaca gaaagaactc
     6001 caacgcgaac ttgcacgcaa aggactccgt aacttccgta cggaaatacg catgaagggc
     6061 tattggagct tgcatcagaa actgaagcgt aagggagatg atattgcgac cattcacgac
     6121 atagcagcac ttcgtgtggt cgtgcagacc gtagacgatt gctataccgc gcttggcgtc
     6181 attcacgcgc gcttccgtcc ccttcccggt gaatttaagg actacatcgc cttcccaaaa
     6241 ccaaacggct atcaatcgct tcataccact gttctcactt ctgacgctgg tgttgtagag
     6301 atgcaggtac gcaccgacga tatgcaccga agagcacagt tcggtattgc ttcccacatg
     6361 tcgtataagc agcttggtaa ggcggctgca aaaggcgcgt ttgaagtgct ttctttcagt
     6421 tggatacgcg atttagtgcc aacgcttctt aacttctcca agaaaccgaa cgtcactcct
     6481 gtcgtgctct cggataaaga acagaagaaa gcaacgcgcg aagagaagcc ctactggctc
     6541 tcggagcttg ctgacgtgca caccaccctt tcagaatcct cagagttcgt tgatcgctta
     6601 aaagaggact tcttctcgca tcgcgtcttc ttattcacgc cgaaaggtga tgtagttgac
     6661 cttccgatgc attcaacgcc tatcgatttc gcctatgcta ttcattcaga tttgggtgac
     6721 catatgattg gcgcaaaagt gaacgcaaag atggtctcgt ttgatacgcc gctacgtaac
     6781 ggggatatcg ttgaaatact acggaaagaa tcggcacacc ccacagcaaa gtggctcgat
     6841 atggcaaaaa cctcagtagc gcgcaagcac attcgctcag cgcttgaggt ggccgaacgt
     6901 ggcacgacga ctcctattaa accggatacg cgccttctcc gtaagcgagc gaacaaaaag
     6961 aaaccaaagt cttaaaggct gaagttctta accgagtaca gttcttcatc actcttctca
     7021 gcaacctctt cggttgttgc agcatccacc ggtgggaggg tttcgtcgag cacttctgtt
     7081 gccttctcgc ttgcttcaac cgcaagcgca gacacgatat tagaaagatc ctctggagag
     7141 aagaattcaa taaggacacg gccgccattt gggcgatttt caatgaccac acgagtgccg
     7201 agtgtttccg ttagtgactt ctctatttcc atcatctctg gcgtcttatc gtgcttacga
     7261 atcttatcgg tagcgatacg gcgagccagc tgctccacca tgcgtactga ggttttcttg
     7321 atgaggattt ctttgaagag cgtgtctcgt tcctccggcc gatcgttcag catgaggagt
     7381 gcgcgggcat ggccttcgct tatgtcctta tctaccaccg cctgctggat agcgtcaggg
     7441 agtgcaagaa gtcgtactga gtttgagacg tactcacgac tgcgacctac ttttgcgcca
     7501 acttcgctgt gagacagacc gaactcttcg acgagctgtg ccagtgcctt ggcgcgatca
     7561 attgcgttta gatcttcgcg ctgcaggttc tcgatgatag cgagctcgag ctttacctta
     7621 ttggattcct cgccacgaat gacgacagga accgatttaa ggccgatgag tttagaggcg
     7681 cgaagacgac gctcaccggc aatgagctca taacgactct caagtccccc gtccgggcga
     7741 ctcacttcgc ttctcgtgac cgtgagcggc tggaggagac cgtactgacg aatggattcg
     7801 gcaagtgact tcaacgcatc ttcgttgaat tctttgcgcg gctggtacgg gttcggtgat
     7861 atcttgtcca cctctaccca gtagatggaa tcgtatttgg tcggctcctg catgatgttc
     7921 actagtttat catgcatcct ctccctgcaa gatgaaaaaa gaagtgcaaa atgctaaggt
     7981 gcttttaggc cgggatggcg gaattggtat acgcagtgga ttcaaaaccc acctcctcac
     8041 ggattgagag ttcgagtctc tctcccggca ccacgttagt ttgaattgac attctttcct
     8101 cagctacgcc acttccctcc gcctcctgct gctatactag ttgcactacc atggcgtacg
     8161 aaggagcacc tgtgaatcct gtgcctgtcg agcccgacaa agaacactct gcttcattaa
     8221 cgaagcaaat gaaggaggca ttggagaaac tggtaaagca acacggagag acttcaaaac
     8281 aggatgctct tgaggaagag ggcgaagatc gcgagccacc gcctgaaata ccttcgtatc
     8341 ttcgctctca cgaagacaca agctttgaag cacgattctt acgccgttgg gtagaagact
     8401 cagtcgtccg ctacgtttca acgtggaagt ttgaagactt cgctaagttc atgaaagata
     8461 agggacgtat ggcacgtggc gtctatgcat taggacttcc aacaagcaat gttggtcccg
     8521 cgagttcacg tgattcgaag cctcaaaagc ttgctgcgaa acgagcaacg atgcgagaag
     8581 gatggcgcaa cacggtccag cacgaatttt atgtggcgtt tcgcactctt atgtgggagt
     8641 tggtgcgtgg tcgttaccac gtggtccacg aagtaactcc tggaaaagta cgtgttgata
     8701 acactcatga tgactcgcac gtcctttccg aaattttgca cgcaatgctt cgtgcagagg
     8761 agccagtgca aggagagaca ggaatgaccc gcgaacagaa gcttgaaata gtcgacatgc
     8821 tcgtacactc cgtgcaggag gaggtagcgg tacttgaaga aagtcttgct cccggctggg
     8881 aagcgcttgc accggctgac gaatctgatc agtttaatat tggtttcttg gatcgggcac
     8941 gcataggaaa attatcagtg cctgagcgtg ctgaacgtat gtatcaaatg atccatctag
     9001 cgaaggaact caatgctgtc gttgatacca cccttgatgg cgcaggtcgc ccgctcataa
     9061 acaatcgtat acgtcttcct gaaaacattt ccgtatcaag gagcgatttt gccgtcatta
     9121 tgaacgagtt cggctttgat ctaggtttta tgagcggcat ggcgcgctat gtgggacgtg
     9181 aattcagcct tcaaaaatcc caaggagtgg cgaagcttga tagcgaaggt ataaaagatt
     9241 tggccgagca taaaaaagca ttccttaaag ccgctgaagc tcttcctgac attcatatct
     9301 tggattcact caatcgtctt gctcaaaccg gacttcttcc cctatataac gaacataatg
     9361 aaataactgc tttacttaag gcacgtaaag acgttgaccc tgcgtacctt tctgagctgg
     9421 ctcaacaatc actcaatatc agtcctgcag tgcgccagga acgatcttcg ccaaaagatg
     9481 cactcgacga cgcaggcgct cttgctacga agtgggagac tatcgcgtcc gaatcaggac
     9541 ctgcagcagc taccgcgaag aagaacgcta ccgctgaact taagaagtat tttgccattc
     9601 acgcaaagaa cgactctata aagctccttg cagaatcgct cgaactattc aagagtgggc
     9661 ctgatattgg aaaaattgtt gcccccataa ttgaaaaacg cctggcgtcg cttgcaagca
     9721 taaacctacg atctgccctc attgagacac gagaacttcg taagatggga tatgacgcac
     9781 tcataaccga taaagcgata caggcgctcg ggctccgagc aaaagacctc gtagaagaga
     9841 tgctcgttcg aaccagtagc caagacacct atcttttctt cagagagctt cttgcaagcg
     9901 gactgggaga atttgtgctt actgagaaag cagcctcgct atggactgaa cgaatacttg
     9961 cacacgatat agagttttcg cgcaccgaag tcctcaaaga ggagttgcag cttggttcct
    10021 agcacctaga tcttccctac caaatccatc cctggctcaa gcgtatcgtc tcctggttcc
    10081 catgacgcag gacacacatt tcccggatgc gcttctacat actgtgctgc ctgaatctta
    10141 cggagcgttt ctgctgcctt acgtccgatg gagttatgcg tcatttccat agagcggagc
    10201 gtaccagctg ggtcaatgat gaaggtaccg cgcatggaca agccttcgtc ttcaacaagc
    10261 tctgttccat cgccttcgat aagggtaccg aatgcataag agatctttcc tgaagggtca
    10321 gcgcccattg ggaattcaat ctgctttatt gcagcagatg tgtcgtgcca tgctttatgc
    10381 gtgaatacgg tatccgttga gaaggatacg acttcagcat taatctcctt aaactttagg
    10441 tagagctttg ccatctcttc aagctccgtc ggacacacga acgtgaagtc ggcaggatag
    10501 aacatcaaaa caagccattt gccgcggaaa tcagagaact tcatcgcctt ggtgtcgtcc
    10561 ttgtagtatg cctcgaactc ataatcgggg accgtctccc ccacctttac cgacgagacg
    10621 tattccttca tcggatcatg aaagtcaccg cagtgctcac aataattgct cttctgtccg
    10681 tttttgttca taaggagatt cgttgcctaa caatgctagt ggaggcatga tagcatccgg
    10741 gacatgtctg gtaaaacacc tatcgtctgc atcatcggcc ctaccgcgtc cgggaaatcg
    10801 ggccgtgcca ttgcgcttgc taaagaacga aacggcgaaa tcatctctgt agattcccgt
    10861 caggtatacc gaatgctcga cataggtacc gagaaaatat caaaagaaga gatgcaggga
    10921 gttccccacc atcttattga tatccgtgac cctaaggagt cgtatagcgc tggcgacttt
    10981 gcgaaagatg ccacacatct catagaggac ataaccaagc aaggaaagct ccccatactt
    11041 gctggcggca gtcacttcta tttcgacgca ctcataaacg gcataccggg agaaacccct
    11101 atggaccccg tattgcgcga atctctggag aaaatgagtg atgaagagtt gtttgaaaag
    11161 attaaagaag gagatccatc gagggcagaa acgattgatc ccaagaaccg tcgtcgcctc
    11221 gtgcgtgcgc ttgagatcat taccctccac ggcaaggtgc cagaacgctc caaagcctct
    11281 ctctacgacg ttgaatggat agtgcttcta ccagaacgcg aagagctccg tgctcgtatt
    11341 gaagcgagac ttaaagagac tctggaaaag ggtctcattg atgaggtgcg tcgcgtccgt
    11401 gactacgtag gcgatgagcg cttgaatgaa ctcggtcttg agtaccgaat tgtcggcgaa
    11461 tatcttcgcg gtgaacgtga tgaagaaacc ctacttcatg cacttactgc aaagctattt
    11521 cagtacgcgc gacgtcaaga ggcgtggatt aagaagttag agtcctcttc tatccgcctc
    11581 cgttaagtga catgtgtggg gccgtgatgc agtgacccgt tcccgtactg cattgtccgt
    11641 ttacgaatct tcccgtaagc aaaagcatat ttgctgctgg cactcctgca tttcgcttcg
    11701 cgttgtagtc ggattgcgag tttgtttcgt attttactgt gcctggccac gccgctattg
    11761 ctgcaagtac ttgcgctgat gaacaggtgc caaaacagct catatcaagg ttattagaac
    11821 cactcgcatg cgtaacagaa gtgtttaccc cagtctcggt gatttgatac tgacttgcgg
    11881 cagagccaag agccgacgcc atctgattaa gacttgagtt tcttcctgca gttgtttggc
    11941 aactattcgc atttttacag ttaaatgcag ttatcggtac aagaccagca cccgttgccg
    12001 tctcacacgc gtcacccgtg ccgtcgttat cagcatcagc ctggttctga tttgcgacaa
    12061 atggacagtt atcaacctga tcctttatta gatcattatc atcatcatcg tctgtgctgt
    12121 ttggttttcc gtcagcatct ttgtcatcat ttggtccagt tccagctcca gtggtgagtc
    12181 ctccatcaga tggagttgat cccgctgggg gagttatcgc aaccaagcac tcttttccgt
    12241 ctgcaagaat cgcattccac gggccatagc tgccttcgtt atagagggtc ttcatcacga
    12301 agtcgacgat gagccatgca cacagagtta tgagtaaccc caccaccgca tttagaagta
    12361 tcgttcttcc ctgttctcgg ttgtgcgcat tcatcgggct gaacatccac aataggcccg
    12421 catatgccgc cacgagcacg aaaataacaa cgccaatgga tatagcgaag ttcatgagat
    12481 tttcaatgac ctgcagtaca cagccaaaat caggggcagt agtttcgcaa tgacactctg
    12541 gcggaatgat agggccgaag aaggtggccg ttgccgccga ggcctgatga ggggtaagcg
    12601 caagaatgcc gaacacgagt accggaagga gaacttttat gaaacgcata gctccagtat
    12661 agggtatttg cgcgtgtaaa tgccttttgc ccacagtgac gcattctgtt aggctaccgg
    12721 tacagggtcc atagtttagt ggtagaacat cggtctccaa aaccgatagt gtaggttcga
    12781 ttcctactgg gcccgctcgt ttgataccgg tttattgaag ccaggtaatc cgtttccatg
    12841 aatcggagag gatataatga aggacatgaa attccttgag cgaataggcc tctctaagaa
    12901 aagtgagaag gtagaagaag ccacaggccc agaagtggta ataaccgaac aggagaagat
    12961 agagcttgtc cgtgatttat cgaaaatttt agagtcactg cgtcctgaca ttgaaagtgg
    13021 agcgataggg tctgtggtag gtattgatcg aagtggacgc cctgttgcgc tcgcccttaa
    13081 gcatgctatc agcgaacgat acaaaacact cggccataag cctattgaaa ctcgattctt
    13141 taatggacta cgcttaggca taaagccagg gcacgacata cgaaagataa atagagtcga
    13201 caagaatctt ttagaagctt tggagacgca tcctctcgac aagactcgca aggtgattat
    13261 tgcggatgac tttattggct ggatggagac tatgccctcg tttattcgag ttttcactaa
    13321 tgccggttat caggtggagg ttgccgctct cggtagccgc ccgtctcatc ttaggggaca
    13381 gaaggacgct actttcaaat ttcgtgattc atacatggtg gccgtaggcg aaccaggcag
    13441 acagcatacc gaacataaac tagagacgta tgaagctcca ttgcaccaag gtgactccct
    13501 cgatggcttt aaaaaagggg ggaaactgag tggcgtccag acagattgga gtggcggcgc
    13561 tcatgcttta aagcaaccga ccactcctgg actgggagcg actatacgac aaggccgcca
    13621 agacataata gaaatagctg aggccgcgct ggctgcatac caacagagca aagatgacac
    13681 cagatcctca tagcggattc cccacgacaa acagggcgtt ttctcggact aataagctta
    13741 acttggttcg aatctcgtcc cataaggccc gctaaacatt tcgcctgtcg tcatctgacg
    13801 acaggcgaag aggttggatc tattcaagat gattcaggaa gctttgggcg cgcgcggttc
    13861 gcatttgcgc gcctccaaga acttccttaa gaacgtgctt agttcgactt atcagcttct
    13921 cgcaaagcga actgcccagt gtggtcgctg ccctgaacgt aggcggctca gcgcaagagt
    13981 agagaaaaaa ctcccgcacc tcgacttcaa cctttgactc cgaccctata tcggcaagaa
    14041 tcatttttac gttgcagtac acgctgtttg cgccggacat ctcagaagta accttgatta
    14101 cctccacact gtccgcgtat gggccgagtt tttcaatggc cgcacggcaa ccgacaacaa
    14161 ggcttacgag cgctagctcg ggaagccaat gcgcagactt cttaatctga gcatcgatac
    14221 tgattgtcgc gtttttcaca gaacacctcc gtggcgccta agacgcctct cattcctacc
    14281 ccacatacga gataggcaat cgtactttgc taggtttttc caaattagtc aactccgttc
    14341 gaatagcgtc ccagtgggcc cgctcaaaaa agctataatt gatgatatgc caaaagtcat
    14401 catcgtcgac gaaaacgata ctcccatcgg acttaaggag cgtgccgact ttttaccagg
    14461 cgatatacat cgagtaaccg cgctatggct caccaactct caaggagaga tcttgttagc
    14521 gcagcgtgct ttcactaaga aacatgcgcc tggcatgtgg ggacctgccg tctccggcac
    14581 ggtagacgaa ggagaaacct acgaatctaa cgtcattaaa gaaactaagg aagagatcgg
    14641 actttcgata tcactcgacg aactgaaaaa aggtccaaag tatcttgctg gagaagggac
    14701 ggagaatggt cgctttgtgc agtggttctc cgcagtacgt gatgtagcaa tagatgagct
    14761 aacacttcct cccgacgaag ttatcgcggc acgctggata acgcttgcag atcttaagcg
    14821 tgaggtactt gaagcgccgg atcggtttgt ttccgctatg agtcgaaccc ctcctatcta
    14881 cgaagccttc taagaaaaac cgccctttcg ggcggttgtt tctttatttc tttgcttcct
    14941 tgaaaacctc gcgctttccg gtgcgcttgt cgaacttttt gagctcaatc ttacgctcta
    15001 ctcccttctt attcttgcgg gtgtagtaga cggtgtcgga cttggtcgac ttgagcttta
    15061 tgagggtatc ctgagacata gacagaatct aacagatagg ccgggtttag gcaacgctag
    15121 actgcttccg ccttagcctt acgaaccctg gacacgctct ttttcacctc aaaatgaggc
    15181 tctccggcct tcatagtgac gtttatagat cctccctcca ttacgccctg gccgaccata
    15241 agggtagcta taggggtcaa aatcttgtcc tggatagcgc gcttaagggg gcgtgcgccg
    15301 tactgggggt tatacccccc ctccgcaagc caggccttca cctcaggtga gagggtaagg
    15361 gtaatgcgct tgccgagaag gcgcttggtg acctcttcca cctgatgctc cacaatcttg
    15421 gtgagggttt cttttccaag aacgtcgaac atgacgatgt catcgaggcg gttcaagaac
    15481 tcaggacgga agttatcctt gagtgagctc attaccttct ccttcatctg ctcgctctgc
    15541 gctgccgcgt cgttaccgct ctgactaaac ccaatgccgg ccatgcgatc catgtactca
    15601 gcaccaacgt ttgaggtaag gacgatgatg gtgttcttga agttcacctt gcgtcctttg
    15661 ccgtcagtga ggtgtccgga gtcgagtacc tgaagcagga tgttgaagac ttctgagtgt
    15721 gccttttcaa tctcatcgaa gagcaccacg gcgtacgggc gatgacgaat gcgttcagtc
    15781 aagcttcctg cctcttcata ccctacgtat cccggagggg caccgataag tttagagacg
    15841 gaatgcttct ccataaactc cgacatatca acacgcacga gcgcatcctt gtcgttaaac
    15901 ataaattccg cgaggcgacg cgagagttcc gtcttaccta cgcccgttgg tccaaggaac
    15961 ataaacgaac caataggacg attcgggtcc gcaataccaa cacgtgaacg cttcacagcg
    16021 tcagcaatta tcttcactgc tttgtcctga cctaccactg ccccggtgag cgcctcttcc
    16081 atgcgtgcaa gctttgcggc ctcttcttca atcattcgag aaaccggaat acccgtccag
    16141 cgagagacca caccagcgat ctctgactct gtgacttctt cgtggagaac acgacgcgac
    16201 ttctgaagcg tcttcaaacg cttcatgcgt acctcaagtt ccttttgaag ggtaggaatt
    16261 ttgccgtagc gaatttcagc cgccgtccca aggtcagcaa tagcttcagc gttctctgcc
    16321 tgtacccgaa gtttatcgag ctcattcttt atggaacgta tcacttcaag cacttctttt
    16381 tcgttcttcc atttaagctc aagttccgag gtcgtctcct ttaagtccgc aatctcagta
    16441 tcaatagctt taatacgttc acggatttcc tttacatgtt cccctgcagt agatgaggtc
    16501 tcttcgtctt tcgtgagtgc ttcgcgctca atagtgagac ggcgtatctt gcggtctgtt
    16561 tcctcaagtg ccggtggttt gttctcaagc gcaatacgga gccccgaagc tgcctcatcg
    16621 ataagatcga tagctttatc aggaaggaaa cggtcagaga tgtagcgggt agagagttct
    16681 acggctgaaa cgatagcgcc gtccgtgata cgcacaccat ggaagagttc gtacttatca
    16741 cgtagaccac ggagaatcgc taccgcatct tctactgacg gctcgtttac atataccggc
    16801 tggaaacgac gcgtgagcgc ggtgtctttt tcaatatgct gctgatattc cttaagcgtc
    16861 gtcgcaccga tgatacgaat ctcgccgcgc gccaacgcgg gctttagcat attagaagca
    16921 tccatcgctc cttctgcagc acccgcccct acgagtgtgt gaagctcatc cacaaagaga
    16981 atcaccttcc cttctgcacg ctctacttct ttcatcaccg tcttcatgcg ttcctcgaac
    17041 tcaccgcgat acttgg
//
LOCUS       DAORIN010000004        15316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_656_length_15316_cov_5.544329, whole genome shotgun sequence.
ACCESSION   DAORIN010000004 DAORIN010000000
VERSION     DAORIN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15316
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_15316_cov_5.544329"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PK109_00350"
     CDS             complement(<1..181)
                     /locus_tag="PK109_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002816334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRH24029.1"
                     /translation="MGKKRIIKKSGRGLDAGRKERALSKLAKRRMEKGVLHVEATFNN
                     TKAVLTDEKGNAVMSS"
     gene            complement(206..586)
                     /gene="rpsM"
                     /locus_tag="PK109_00355"
     CDS             complement(206..586)
                     /gene="rpsM"
                     /locus_tag="PK109_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRH24030.1"
                     /translation="MRIAGVTIPDNKQLAYSLPMIFGIGPTRALDILKKTGVPANKKG
                     SELTSEEEQAIRSLAEGYLIEGELRREIAQNVKRLKDIGAARGIRHQRRLPVKNQRTK
                     TNSRTVRGNTRKTMGSGRRSLEKT"
     gene            complement(679..795)
                     /gene="rpmJ"
                     /locus_tag="PK109_00360"
     CDS             complement(679..795)
                     /gene="rpmJ"
                     /locus_tag="PK109_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011820532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRH24031.1"
                     /translation="MKVRASIKKQQTGDIIVRRKGRLYRINKLKPRRKARQG"
     gene            complement(800..1018)
                     /gene="infA"
                     /locus_tag="PK109_00365"
     CDS             complement(800..1018)
                     /gene="infA"
                     /locus_tag="PK109_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRH24032.1"
                     /translation="MEEERAQGAPTGTVEEVLPNSLFRVRMESGELLLAYLAGKMRLH
                     RIRVLVGDKVELVLDPYGGRARIVRRNS"
     gene            complement(1068..2360)
                     /locus_tag="PK109_00370"
     CDS             complement(1068..2360)
                     /locus_tag="PK109_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24033.1"
                     /translation="MPKTITDIIPPSRRRAMEEAGQSQAYAPASYQPPADMPPMPPAP
                     PRDTTPMGSMGRPRSSFPWRLATAAVVVVVIAIGAIYAFSGAKVTVTPTMNVATVGGE
                     FTATPSAGELPFEVITVEKVGTQPVKAEGTENANDPSQGMITIYNGQEKVQELIKNTR
                     FATPDGLIFRIHDSVKIPAGSPTAPGQLQVTVYADAGGEKYNIGPSTFTLPGLKGSAV
                     YDLVYAKSTGSMSGGFSGVRPSVSGATRDAQASTIEAGLDKDLREEIGEKIPEGYILV
                     PGAVFISYAQLPDAAGENNNVNVQEKGTANAFVFPKAALAKAIAYQSVGTYAGQPVTI
                     ESAERLTFAPKNGEAPVPGADSATFTLSGTVDITWVVDPAKISGSVAGKSRQAAQTIL
                     AGFPEVEKAVLSLKPFWAGEFPEDPAEIKVLVETEDAD"
     gene            complement(2451..3569)
                     /locus_tag="PK109_00375"
     CDS             complement(2451..3569)
                     /locus_tag="PK109_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24034.1"
                     /translation="MSLFGSKNKSKAVVLIDVTSSSIGGAYAIVSESAPAALLYTTRV
                     ALPTTGADVPSELMLRALDEVLQDLRINGAPILHRRTGSGSVQAVVASVGAPWQTTNI
                     RTETVVEEKPFLFTDAIIAGAKKKGEIPVGRYSSEESVLATLLNGYETPQPVGKRAKR
                     AEIILLGATMEEETAKLMRRALSSFSALHEVRFSGLASVVSTAVALAFPHEKEYLALR
                     VSGEVTELVFVKRGHPMFVSHIPSGLNVFARAAKSTGYASFPDGGDIIDRTKSTELDA
                     GLKEAEEAWTTAVATCLKEFTATHALPRTLFLIVDKEAREFIRRVLDVPQVHALWLSD
                     EPLATIALECSHFSKNMACETGVEEDLMLGLLALVARS"
     gene            complement(3584..4102)
                     /locus_tag="PK109_00380"
     CDS             complement(3584..4102)
                     /locus_tag="PK109_00380"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRH24035.1"
                     /translation="MRIDIVGITGSGKSTLASRLSQKLGIPHIQLDTFWFEAGGRKGP
                     YDTPNIDAVRAHVKTAVEKAVQQDSWISDGTYLHAQEVIAPAADVIIFLDTPLWTRLV
                     AHARRTFFEPKRHKHLTVWDEISFFKEIITRTYRSRPKLITFLEGYIDKTIVLRSYKE
                     IDEYVIGGAARK"
     gene            complement(4145..6064)
                     /locus_tag="PK109_00385"
     CDS             complement(4145..6064)
                     /locus_tag="PK109_00385"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRH24036.1"
                     /translation="MTVSEVRARYLAFFKARGHAIIPSAPIVPGNDPTTLFTSSGMQP
                     LVPYLLGKDHPAGNRIANSQMSFRAGDIEEVGDNRHTTFFEMLGNWSLGDYFKKEQLP
                     WIFEFLTSKEEGLGLDPHKLYVTVFAGDADTGMEEDLEAVGIWKELFKAKGIDAEYSN
                     IGSEEAGYQKGMRGNDRIFAYDAKKNWWSRAGVPGKMPAGEPGGPDSEVFYDFGLPHD
                     PVWGAECHPNCDCGRFVEIGNSVFMQFVKQDDGSFKPLPKQNVDFGGGLERLTFATIN
                     SPDVFDIDIFAAARVVIAERSGKSYDDEATTKKAFRIILDHMRAASFMLAEGITPSNT
                     EAGYVLRRLIRRAIREADRLGIKDAVLADVAKGYGEAYADHYPHVAENADKIRDELAK
                     EEEKFRKTLSQGLKEFEKLAAKHEVTAEDAFVLFTTYGFPVELTEELAKERGITLNAE
                     AVAAKMKEHQDTSRAGGAQRFAGGLADHAEQTVQYHTTHHILLAALQKVLGPEVKQRG
                     SNITSERLRIDFASPGKMTDEQKAEVEKIVNDIIYQDLPMTRSVMPREEAEKLGAQME
                     FGVKYPDMVSVYSLGPIGATEADPKIDEAFSLEFCGGPHVSNTSEIHQGGKKFKIKKE
                     EAVAAGIRRIKAVVE"
     gene            6130..8094
                     /locus_tag="PK109_00390"
     CDS             6130..8094
                     /locus_tag="PK109_00390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRH24037.1"
                     /translation="MKKNWGFDVRDMDTMVRPQDDFYHYAAGGWLARNPIPESEARWG
                     SFMALRYDTDRQLRTILSELETMKRVTKGSPEQLIRDFYRSGMDMKRRNALGAAPLAP
                     FLKNIEGIKTHTELLTQIAALHRMGVGVLWGTAVDQDSKNSEKYLLHIYQDGLGMPER
                     DYYLKNDPESKRVRDAYLPYVERVLRLMGTKPAKAAGAAATILRIETALAKAWMSKED
                     RREAEKVYHKKTVRELARLAPAIDWPRYFKNTGAGTLSSVIVMHPEFLAACSTLLTEV
                     SLEDWKTYLSFHVTNDYAGYLSATFVRTSFSFYGTVLSGTKVMKPLWRRTMNAVNGSL
                     GEPLGRIYVHKHFSKDAKYKMELLVADLFEAYEARLKNIDWMTPTTKKKALKKLRAMN
                     RKIGYPDKWKSYAGLTIRPDDFAGNAIRSELFEHRREMKKLAKPIDRTEWFMFPQTVN
                     AYFAPNLNDIVFPAAILQPPFFNLEADDAVNYAAIGSVIGHEITHGFDDQGSKYDEKG
                     NLKSWWTLADRARFEKKASIIVKQYDAYTVADGVHVNGKLTLGENIADLGGIAIAYDA
                     YQLRLKKTGRAVLGGFTPEQRFFLGLTLFERESARPEYQKMQVLTDPHSPAKYRINGP
                     VGNVPAFYEAFTVAKGDALYRDPSVRTTVW"
     gene            8128..8508
                     /locus_tag="PK109_00395"
     CDS             8128..8508
                     /locus_tag="PK109_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24038.1"
                     /translation="MKNQTLIGIVIIVIVLVAGAYVLTLPASAPTVEQAVEQEGKLKA
                     ANFEGTLTEVNMGCAYDAECYAVVDGKHITLLKGRNRDVSGPVDLDVLGANIGKTVEV
                     YVHDLGDGTYTLYGSEGFYVRPKN"
     gene            complement(8510..9172)
                     /locus_tag="PK109_00400"
     CDS             complement(8510..9172)
                     /locus_tag="PK109_00400"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRH24039.1"
                     /translation="MKVAIEPSWKDALKEEFDKLYFAELASFVKEEYTSTTVYPSPKF
                     IFRAFELCPFDAVKVVILGQDPYHGPGQANGLSFAVNEGMRAPPSLQNIFKEVESDTG
                     KPSVGGTDLSRWAKQGVLLLNATLTVRARTAGSHQNRGWEQFTDAVIRALSEKREHLV
                     FILWGNYAKQKGIHIDRTKHLVIESPHPSPFSAYNGFFGSKPFSKTNDYLVTNGETPI
                     AW"
     gene            complement(9491..9895)
                     /locus_tag="PK109_00405"
     CDS             complement(9491..9895)
                     /locus_tag="PK109_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24040.1"
                     /translation="MSETVPHQRVNRAEEFEEARAAGFRPLSDYIAAIGNRAGARVSD
                     MNVRKFLKMTLGIPAESTSADIEALIPANHPLRSHLRFPDGKLFFDQYLFMRVLETLS
                     REWKDHPQTTVRSTVRKLAPYLENLKKELGVE"
     gene            complement(9892..10152)
                     /locus_tag="PK109_00410"
     CDS             complement(9892..10152)
                     /locus_tag="PK109_00410"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRH24041.1"
                     /translation="MKSDFTERVRKVVRSIPKGKTMTYGLVAHRAGKPGAARAVGTIM
                     ANNDDKTVPCHRVIRSDGKIGGYNNINGPSKLELLRAEGVAI"
     gene            complement(10155..10937)
                     /locus_tag="PK109_00415"
     CDS             complement(10155..10937)
                     /locus_tag="PK109_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRH24042.1"
                     /translation="MAKWSKSFDPSSTEPFTISRSKVDMFTECKRCSYLDLRLGVKRP
                     SMPSFTLNNAVDELFKREFDIHRAAGSTHPLMKKYGIDAVPMKDERLDEWRDALRRGI
                     KTHHKESNLILRGGIDDVWQTPDGELIIVDYKATSKKIGPSTEDDLYDSYKKQLEVYQ
                     WLFRQNDFKVSSTGYFVYANGKSDVDAFDAKLEFDIVLIPYTGNDGWVEGVLMDMRKT
                     LSSDEIPEVGISFGGGPCEHCTYREAAGKILLDIHKKKKAAK"
     gene            complement(10948..11778)
                     /locus_tag="PK109_00420"
     CDS             complement(10948..11778)
                     /locus_tag="PK109_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRH24043.1"
                     /translation="MTDILKADGTTEPFDPSKLEASLRRSGASAQAAERIGHVITSTI
                     VSGIDTHEIYRRAFALLRDEERAVAARYSLRRALFELGPSGHPFEDFVSALFEKEGWN
                     VEWRKIIPGKCVSHEVDVYATRGNEHLAAELKYHNDPNYKTDVKVALYVKARFDDIWQ
                     CDPKVSATCPVDTGFLITNTKFTETAIQFAKCSGIGLLGWSYPTEGNLYDRIIAAGIF
                     PVTALTQLRKSEKRLLIDQGIVTSAQLRGKREALRALAIPPERIGLIAAEIEALETPV
                     "
     gene            complement(11779..12813)
                     /gene="mnmA"
                     /locus_tag="PK109_00425"
     CDS             complement(11779..12813)
                     /gene="mnmA"
                     /locus_tag="PK109_00425"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRH24044.1"
                     /translation="MNVKGKRIFVGLSGGVDSAVTAALLKEAGADVVGVFIKGWYPPN
                     LTCTWAADRRDAMRVAARLFIPFHTLDASAAYKKSVIDYLLSEYSQGRTPNPDIMCNR
                     DVKFGAFYSFAMEQGADAIATGHYAIAKDGHVYRGVDPEKDQSYFLWAVAKEALEKTL
                     FPLGTYKKSEIRTLAKRFNLPVAEKKDSQGICFLGAVSVDDFLRDEFHPEPGSAVDRE
                     GKTIGTHDGAVLRTLGERVALTDAAPGPWYVVRKDIAKNLLVVSKEKMTVVQDATIRI
                     KHTNWFTPVTNNEPLEAQYRYHGPKVSGTFHISTDTFTPTDELPEPIASGQSLVIYRD
                     AECVGGGIIT"
     gene            complement(12838..13827)
                     /gene="mltG"
                     /locus_tag="PK109_00430"
     CDS             complement(12838..13827)
                     /gene="mltG"
                     /locus_tag="PK109_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRH24045.1"
                     /translation="MVLFSYLPFVLEALYLHIVRKGRFYAGLAFVLFVMLPAWILLIP
                     ASSFPHGALVEVNEDMTFSQTATQLENRDIVENAVLFKVLARVTGVDTSVKAGRYLFT
                     EPQGVAVVLYRLATGDSGIRAVRITFPEGITVREMGEILGTSLPGFATDTFVTNATPL
                     EGYLFPDTYAFYADVTPEEVITRMRARFDEVWSEIAVDTPEPKERIVIMASILEKETK
                     VGEDRQVVSGILWKRIDIGMALQVDAVFGYIKGVDTYHPSGDDLEIDSPYNTYEYPDL
                     PPGPIGNPGKDALQAALHPIASDYLYYLSDAEGVVHYAEDFEGHKKNKEQYLR"
     gene            complement(13829..15238)
                     /locus_tag="PK109_00435"
     CDS             complement(13829..15238)
                     /locus_tag="PK109_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24046.1"
                     /translation="MSSGGGGHSTGELELPGLHALHEMLESSNESGGFIVPGWLLILV
                     YFICAGVFAAQAPVQSAQNWDMLLTLGPIWLPIVLGRFALMRFMNMRQMNFNYKNPFV
                     LLEMRIPREINKTPRAMETVLASLNIGPGTGTWLKKYWWGRTRPWWSLELASIEGNIH
                     FYINTRKNMRRALESYMYAQYPEIELIEAVDYARLRDPLHEPYQMFACEYAYGLDDPF
                     PIRTYVDLGLDKPGDKPEEISDPFAQILELFGSLGPGEQLWTQIIIRETKGEKYRGRI
                     NPDNGKPYSWRERGREVIEALRAKTVNRRQTVDAEGHVQEVEGFPNPNEFQKELIGAV
                     ERNIDKPAFDVGIRSIYSAPADKYHGTMNAFTANMFKVFISQQYNKINPAPLWSEKFN
                     DYPWEDIGGHRQRAEMHEVLEAYRARSYFHPPYRGPWMIMSTETIATIYHVPSSTSKA
                     PGLARIGSTTSAPPPNLPL"
BASE COUNT         3529 a         4079 c         3959 g         3749 t
ORIGIN      
        1 aggatgacat aaccgcattg cccttttcgt cggtgagcac tgccttcgtg ttgttgaagg
       61 ttgcttcgac atggagcacg cccttttcca tgcggcgctt cgcgagcttt gaaagcgcgc
      121 gttccttgcg tcctgcatcg aggccgcgtc ctgacttctt gatgattcgt ttctttccca
      181 taaatagtta aataatgaat caggattaag ttttctcgag cgagcggcga ccagaaccca
      241 tggtcttacg cgtgttacca cggaccgtgc gggagttcgt cttcgtgcgc tggtttttca
      301 ccggaaggcg gcgctggtgg cggataccac gagcagctcc aatatccttc aaacgcttca
      361 cgttctgcgc gatttcgcgg cggagctctc cttcaatgag gtagccctca gcaagggaac
      421 gaatagcctg ctcctcttcc gaggtgagct cgctcccctt cttgttagcg ggaacgccgg
      481 tcttcttcaa aatatcgagt gcacgcgttg ggcctatgcc gaagatcatc ggcaatgagt
      541 aggccaactg cttattatct ggaatggtga cgcctgcgat acgcatgtcg ttggtaaatt
      601 cgtttggtta agtaaatgtc ttaaaaagta tcctcccggt ctcccggaat taagatgaag
      661 gaccgaagtc ctttatcgtt aaccttgtcg tgccttgcgg cgaggcttca gcttatttat
      721 gcggtacaag cgacccttgc ggcggacaat gatgtccccc gtctgttgct ttttgatgga
      781 tgctcgtact ttcatagttt tagctgttgc gcctcacaat gcgggcccgg cctccataag
      841 gatcgagcac aagctcgact ttgtccccta caaggacgcg gattcggtgc aagcgcatct
      901 tgccagcaag atacgcaagc aagagctctc cggattccat acggacccga aacagggaat
      961 tcggcagcac ctcttctacg gtgccggtcg gtgcgccctg agctcgctct tcctccattg
     1021 cgttgcctga ttaatgtaac agataggcct tagggcgtca aacgcgccta gtcggcatct
     1081 tcggtctcaa ccaagacttt gatctctgcc gggtcctccg ggaactcccc tgcccagaat
     1141 ggcttcaggg aaaggaccgc cttttcaacc tcagggaacc cagcaaggat ggtctgcgct
     1201 gcctggcgtg acttcccggc aactgaacca gagatcttcg caggatcaac gacccatgtg
     1261 atgtcgacag tgcctgaaag cgtgaaggta gcgctatcag ctcctggaac tggagcttca
     1321 ccattcttcg gcgcaaaggt aaggcgctct gctgattcaa tcgtgacggg ctgaccagcg
     1381 taggtaccta ctgactggta tgcaatagct ttagcgagcg ctgccttcgg gaacacgaat
     1441 gcgtttgcgg ttcccttttc ctgcacgttc acattgttgt tctcgccggc agcgtcagga
     1501 agctgtgcgt aggaaataaa gacggcacca ggaacaagga tgtacccctc aggaatcttc
     1561 tcgccaatct cttcgcgaag atccttatca aggcctgctt caatcgtgct tgcctgcgcg
     1621 tcacgagtag cgccagaaac ggaaggacgt acgccggaga agccgccgct catggagccg
     1681 gtagactttg catacacaag gtcataaacg gcagaaccct taagaccagg aagcgtgaag
     1741 gtggatgggc cgatgttgta cttctcccca ccggcgtcag cgtataccgt tacctggagc
     1801 tggcccggag ccgtaggaga gccagctgga atctttaccg agtcatggat gcggaagata
     1861 aggccgtcag gggtcgcaaa gcgggtgttc ttaatgagct cctgcacctt ctcttgaccg
     1921 ttatatatgg tgatcatgcc ctgagatggg tcattggcat tttctgtgcc ttcagccttc
     1981 actggctggg taccgacttt ttctaccgta atgacttcaa acggaagctc gccagcgctt
     2041 ggggtcgcgg tgaactcgcc gccaaccgta gcgacgttca tggtcggcgt gaccgtaacc
     2101 ttcgcgccag agaatgcgta aatcgcacct atcgcaataa caacaacgac tactgccgct
     2161 gtagcaagac gccacgggaa cgaagaacgt ggtcgaccca tgcttcccat aggcgtggtg
     2221 tcgcgtggcg gcgctggtgg catcggtggc atatccgccg gcggctggta gcttgccgga
     2281 gcgtaggctt gtgactgccc tgcttcttcc atggcgcggc gacgcgatgg cgggatgatg
     2341 tcagtgatgg ttttaggcat agcgatagat gggttggtaa tccgtaacag tatacgccat
     2401 atcgtagccc gataccttta ggccgagcaa accactgacc gggtataact ctaggaacgg
     2461 gcaacaagtg ccaagagccc gagcatcaaa tcttcttcaa ctccagtttc gcacgccatg
     2521 ttctttgaga aatgcgagca ctcaagtgca atggtggcga gtggttcgtc cgaaagccag
     2581 agcgcatgca cctgcggcac atcgagcacg cgtcgtatga attcacgcgc ttctttatca
     2641 acgatgagga agagggtgcg cgggagtgcg tgggttgccg tgaactcttt aaggcaggtt
     2701 gcgacagcgg tcgtccatgc ttcctctgct tcttttagtc ctgcatcaag ttcggtactc
     2761 ttggtgcgat caatgatgtc gccgccgtca ggaaatgatg cgtaaccggt agattttgca
     2821 gcacgagcga agacgtttaa tccagaagga atatgcgaca cgaacatcgg gtgtccgcgc
     2881 ttcacaaaca caagctcagt cacctctccc gagacgcgaa gtgcgagata ctctttttca
     2941 tgcgggaaag ccaaagcaac tgccgtagaa acaacggagg caaggcccga aaagcgcacc
     3001 tcatgaagtg cgctgaagct cgaaagtgct cgacgcatca gtttcgcggt ctcttcctcc
     3061 atagtggcgc ccaagaggat gatttctgcc cgctttgccc tctttcctac cggctgtggc
     3121 gtctcatagc catttaaaag cgttgcgagc acggactcct ccgaggaata ccttccgaca
     3181 gggatttctc ccttcttttt agcgcctgca atgatggcgt cggtaaagag gaatggtttc
     3241 tcctcgacta ccgtttccgt acgaatattc gtagtctgcc agggagctcc aacagaagcg
     3301 acgactgcct gtacgcttcc agaacccgtg cgacggtgga gaatgggagc gccatttatc
     3361 cgcaaatcct gcaatacttc gtccaaggcg cggagcataa gttcagaagg gacatcagcg
     3421 cccgtagtgg ggagcgccac acgtgtggtg tagagaagag ctgcaggcgc actttctgaa
     3481 acgatggcat acgcgccacc gatagatgat gaagttacgt cgataagtac gacggccttt
     3541 gatttatttt tcgaaccaaa aagggacata gtcctattct actctatttg cgagcagcac
     3601 cgcctatgac gtattcatct atctctttat aggatcgcaa aacaattgtt ttatctatat
     3661 agccttcaag gaaggttatg agcttaggac gacttctata ggtacgagta atgatttctt
     3721 taaagaagct aatttcatcc cataccgtca gatgtttatg tctcttgggt tcaaaaaatg
     3781 ttctacgagc atgagccacg aggcgtgtcc ataaaggggt gtctaagaag ataatcacgt
     3841 cagcagcggg cgcgataact tcctgcgcat gtaaatacgt tccatcggat atccatgaat
     3901 cctgttgtac tgccttctca actgcagttt tcacgtgtgc acgtactgca tctatgttcg
     3961 gtgtgtcata aggcccttta cgtcctcctg cttcaaacca aaaggtatca agttgaatat
     4021 gaggtatgcc taacttttga gaaagtcgtg aagcaagcgt agattttccg cttccagtaa
     4081 ttccgactat atcaatccgc atgcgatgag tataacgttt ggttggcgga ccaggattcg
     4141 aacactactc cactaccgcc ttaatacgcc gtataccggc agctaccgct tcctccttct
     4201 taatcttgaa cttcttcccg ccttggtgaa tctcactcgt gtttgatacg tgtgggcctc
     4261 cacagaactc aagtgagaac gcttcatcga tctttggatc tgcttccgta gcaccgatag
     4321 ggccgagaga gtatacggac accatgtctg ggtacttcac gccgaactcc atctgcgcac
     4381 caagcttttc tgcctcttca cgcggcatca ccgaacgagt catcgggaga tcctgataga
     4441 tgatgtcatt cacaatcttt tctacctctg ctttctgttc gtcggtcatt ttccctggag
     4501 aagcaaagtc gatgcgcaag cgttcactcg tgatattcga gccacgttgc ttcacttctg
     4561 gacctaagac tttctgaagc gctgcaagga ggatgtggtg ggtagtgtgg tattgcaccg
     4621 tctgttcagc atggtcggca agtcctcccg caaagcgctg tgcgcctcca gcacgagagg
     4681 tgtcctgatg ttctttcatc ttcgccgcta ccgcttcagc attaagcgta ataccacgct
     4741 ctttagcgag ctcttcggtg agttctaccg ggaacccgta ggtagtgaag agtacaaaag
     4801 catcctctgc cgttacttcg tgctttgccg caagcttttc aaactcttta aggccttggg
     4861 agagcgtttt acggaatttc tcctcttctt tcgctagctc atcacgaatc ttgtcagcat
     4921 tctctgctac gtgcgggtag tgatcggcat acgcttcgcc atatcccttc gcaacgtcag
     4981 ccagcaccgc gtctttaata ccaagacggt ctgcttcgcg tatagcgcga cgaatgagac
     5041 ggcggagcac gtagccggcc tcggtgttcg acggcgtaat gccttcagca agcatgaaag
     5101 aggcagctcg catatggtcg agaataatgc ggaatgcttt cttagtcgtc gcctcgtcgt
     5161 cgtatgactt tcctgagcgt tctgcgatta ccacgcgtgc tgcagcgaag atatcgatat
     5221 caaatacgtc aggggagtta atggtagcga aggtgagacg ctcaagtcct cctccgaagt
     5281 ccacgttctg cttagggagt ggtttgaatg agccatcgtc ctgcttcacg aactgcatga
     5341 acactgagtt tcctatctct acgaatcggc cacagtcgca gttcggatga cactctgcgc
     5401 cccagactgg gtcatgcggg agcccgaagt catagaacac ttcggagtcg ggtcctcctg
     5461 gctctcctgc gggcatcttg cctggcactc cggcgcggct ccaccagttc ttcttagcgt
     5521 cgtacgcaaa gatacgatca ttaccccgca tacctttctg gtatcccgcc tcttcgcttc
     5581 caatgttgga atactccgca tcaatccctt ttgctttaaa gagttccttc caaatcccta
     5641 ccgcttcaag gtcttcttcc attcctgtat cagcgtcgcc ggcaaagacg gtgacataga
     5701 gcttgtgtgg atcgagcccg aggccctctt ctttagacgt aaggaattca aagatccacg
     5761 gaagctgttc ttttttgaaa tagtcgccaa ggctccagtt accgagcatc tcgaagaagg
     5821 tcgtgtggcg gttgtccccg acctcctcaa tgtctcccgc acggaagctc atctgggaat
     5881 tcgcgatgcg gttccctgcc ggatgatcct tccccaggag gtatggcacc aagggctgca
     5941 tgccagacga ggtgaagagg gtcgtcgggt cgtttcccgg gacaatagga gcagacggaa
     6001 taatcgcgtg tccgcgtgct ttaaagaagg cgagatagcg tgcccgaacc tcagaaacgg
     6061 tcatgaaaga aaagatagca cgacggcgcg ttcatggcat attcttcgtg ccttccctat
     6121 aatcacttta tgaaaaagaa ctggggattt gatgtgcggg acatggatac tatggtgcga
     6181 ccacaggatg atttttatca ctatgctgct ggcggatggc tggcccgtaa tcctatccct
     6241 gagtcagaag cacgctgggg gtcgttcatg gccctccggt atgacaccga tcgtcagctc
     6301 cgcaccattc tttctgagct tgaaacgatg aagcgggtaa cgaaaggcag tcccgagcag
     6361 ctcattcgcg acttctatcg ctcaggcatg gatatgaagc gccgtaacgc actgggtgcc
     6421 gctccacttg cgccctttct aaagaatatt gaaggtataa agacgcatac agagttactt
     6481 acgcagatag cggcgcttca tcgcatgggt gtaggggtgc tctggggtac tgccgtagac
     6541 caagactcaa agaacagtga gaagtatctc cttcatatct accaggatgg ccttggtatg
     6601 cctgagcgtg attactatct caagaacgac cctgaatcca agcgtgtgcg cgacgcatac
     6661 cttccctatg ttgagcgtgt gctccgcctt atgggtacaa aaccagcgaa agctgcagga
     6721 gcggctgcaa ccatactccg gattgagacc gcactcgcaa aagcatggat gagcaaggag
     6781 gatcgtcgtg aagcggagaa ggtgtaccac aagaaaacgg ttcgagagct tgcacgcctt
     6841 gcaccggcta tcgactggcc gcgctatttc aagaacacgg gggcaggcac cctttcatcg
     6901 gtcatcgtaa tgcatcctga gttccttgcc gcatgctcaa cactgctcac ggaggtctca
     6961 cttgaagact ggaagacgta tctttccttc catgtcacca atgactatgc cggatatctt
     7021 tcagccacct ttgtccgtac gagcttctcc ttctatggca ccgtactctc cggtaccaag
     7081 gtcatgaagc cgctgtggcg tcgtaccatg aacgcggtga atgggagctt gggtgaaccg
     7141 cttggtcgta tctatgtaca taagcacttc agtaaggacg cgaagtacaa gatggaactt
     7201 ctcgttgcgg atcttttcga agcgtacgaa gcacgcctta agaatatcga ttggatgacg
     7261 ccaacgacga agaagaaggc gctcaagaaa ctccgtgcta tgaaccggaa gataggatat
     7321 ccagacaagt ggaagtcgta tgctggtctc accattcgtc cggatgattt cgcaggcaat
     7381 gcgatacgct cagaactgtt tgagcatcgt cgtgagatga aaaagcttgc aaagcccatt
     7441 gataggacgg agtggttcat gttcccacag acggtgaacg cgtactttgc gccgaatctt
     7501 aatgacatcg tcttccccgc cgctattctt cagccaccct tcttcaacct tgaggcggac
     7561 gacgccgtga actatgcggc cataggctcg gtcatcggtc acgaaataac ccatggattc
     7621 gatgatcagg gttctaagta tgacgagaaa ggcaacctta agagctggtg gaccttagcg
     7681 gaccgtgctc ggttcgagaa gaaagcgtca attatcgtta agcaatacga cgcgtatacc
     7741 gttgccgacg gcgtgcacgt aaacggtaag ctcacgcttg gagagaacat cgctgaccta
     7801 ggcggcattg ctatcgcata tgatgcctac cagctccgcc tcaagaagac gggacgcgcc
     7861 gtgctcggcg ggtttacccc agaacaacgg ttcttcctgg gtctgactct ctttgagcgc
     7921 gagagtgcgc gccccgaata tcagaagatg caggtactta ccgacccgca ctcacctgcg
     7981 aagtaccgta tcaacgggcc tgttggtaac gtccccgcct tctatgaagc cttcacggtc
     8041 gctaaggggg atgcgctcta tcgcgacccc tcagtgcgga cgacggtgtg gtagtaatgt
     8101 ttctgctatc atttcgtcat accctctatg aaaaaccaaa ccctgatcgg catcgtcatt
     8161 attgttatcg tgctcgtcgc gggcgcctac gtcctcactc ttccggcctc cgctcctact
     8221 gtggagcaag cagtagagca ggaagggaaa ctcaaggcag cgaacttcga aggtacgctc
     8281 accgaggtaa acatggggtg tgcctatgac gctgagtgct atgcggtagt cgacgggaag
     8341 cacataaccc tactcaaagg tcggaatcga gacgtgagtg gtccggtaga tctcgatgta
     8401 cttggagcaa atattggaaa gacggttgag gtatacgtgc acgaccttgg tgacggcacg
     8461 tacaccctct acggaagcga aggattctac gtacgcccta agaactaagt caccacgcta
     8521 tcggcgtctc tccgttcgtt acgagatagt cattcgtctt gctaaacggc tttgagccga
     8581 agaaaccgtt gtatgcggag aacggggacg gatgtggtga ctctatgacc aggtgtttgg
     8641 tgcggtctat gtgtatgcct ttttgcttgg cgtagttgcc ccagagaatg aatacaagat
     8701 gttcgcgctt ctcagaaagc gctcgtatca cagcatcagt aaactgttcc caacccctat
     8761 tctgatgtga tccagcagtg cgggcgcgta cggtaagggt ggcgttcaag aggaggactc
     8821 cctgcttcgc ccagcgtgat aaatcggttc cacccactga tggctttccc gtatcgctct
     8881 caacttcttt gaatatgttt tgaagcgacg gcggagcacg cattccttca ttcacggcaa
     8941 aggagagacc gttagcttgt cctggcccgt gatacggatc ttggccaagg atgaccacct
     9001 ttaccgcatc aaatggacac aactcaaatg ctcgaaaaat aaacttcgga gacggataca
     9061 ccgtcgttga cgtatattcc tccttaacaa acgacgcgag ctctgcaaaa tacagtttgt
     9121 caaactcctc tttcagtgcg tccttccacg agggttctat agcgactttc atgatcggga
     9181 gtatagcaaa atacgtgtaa aggccgcggc ttgcgtcgcg gccttaccag tgatgcccgt
     9241 tacgggcgtc gcagcatcgg tttcgaaatg tacggataaa tccagtatcg gcgagcatcg
     9301 gcagtatagg cgcgtaacca gatgctttgc catgcaaacc aagcatcgat agccatcatg
     9361 cttatatctg cagcagccat cgggcgtacg gacatttgat ccgtagttcg ggaactagtc
     9421 atgttcagtc ctccgctgta agtatgggcg cgtccgacag aagcacaatg cagcgggtgt
     9481 gtagagtatc ctattcaaca ccaagctctt tcttcagatt ttccaggtag ggagcgagct
     9541 tccgtacagt tgagcgtacg gtggtttgcg gatgatcttt ccactcccta gacaatgttt
     9601 ccagcacacg catgaatagg tactgatcaa aaaatagttt tccatcagga aaacgaaggt
     9661 gagagcggag aggatggttg gcaggaataa gtgcttctat atctgcactc gtgctctcgg
     9721 ccggaatacc taaggtcatt ttcaagaatt ttcgtacatt catgtccgag actcgagctc
     9781 cagcacgatt tcctatcgcg gcaatatagt cagatagagg acggaatcct gcggcacgcg
     9841 cctcttcaaa ttcttccgct cggtttactc tctgatgcgg tactgtttcg ctcatatagc
     9901 aactccttcc gctcgaagca attcgagctt tgaaggccca tttatgttgt tatagccacc
     9961 gatttttcca tcagaacgta taacgcgatg gcacggtacg gttttatcat cgttatttgc
    10021 catgatggtg cccaccgcac gtgctgctcc tggtttacct gcccggtgcg caacgagccc
    10081 gtacgtcatc gtctttcctt tcgggatgct gcgtaccacc ttcctgaccc gttctgtaaa
    10141 gtcactcttc atagctattt cgccgctttc tttttcttat gaatatcgag aagaatcttc
    10201 cccgccgcct ccctatacgt gcagtgctcg cacggtccgc caccaaacga gatacctacc
    10261 tcaggaatct catcgcttga aagagtcttg cgcatgtcca tgagtacacc ttctacccag
    10321 ccatcgttac cggtatacgg gataaggacg atatcgaact caagttttgc atcaaaggcg
    10381 tcgacgtccg actttccatt ggcatacaca aagtaccccg ttgatgagac cttgaaatcg
    10441 ttctgacgga agagccactg atatacctca agctgcttct tatatgagtc gtagagatcg
    10501 tcttcggtag aaggtcctat tttcttcgag gtggctttgt agtcaacgat gatgagctcg
    10561 ccatcaggcg tctgccacac gtcatcgata ccgccgcgca gtataagatt cgactcttta
    10621 tgatgcgttt tgatgccgcg gcgaagcgca tcgcgccact catcgagccg ctcatctttc
    10681 ataggaacgg catcaatgcc gtatttcttc atgagagggt gcgtgctgcc tgcggcacgg
    10741 tgtatatcga attcccgctt aaacagctcg tccacagcgt tattgagcgt gaaggacggc
    10801 atggaggggc gcttcacgcc aagacgtaaa tcaaggtacg agcagcgttt gcattcggtg
    10861 aacatatcca ccttgctgcg acttatcgtg aacggttctg tcgaagaagg gtcgaacgat
    10921 ttagaccatt ttgccattcc ccctattcta cactggagtc tccaaagcct ctatttctgc
    10981 cgctatgagg ccgatgcgct caggcgggat ggcgagcgct cggagtgcct cgcgcttccc
    11041 gcggagctgg gcgctcgtca caatgccctg gtcgataagg agccgcttct ctgacttacg
    11101 tagctgggtg agtgccgtta cggggaatat gcccgcagcg atgatgcggt cgtagaggtt
    11161 cccttcggtc gggtaactcc accccaagag cccgatgcca gaacacttag cgaactggat
    11221 ggcggtttcg gtgaacttgg tgttcgtaat aagaaaccca gtatcaacgg gacacgtcgc
    11281 ggagaccttc ggatcgcact gccagatatc gtcgaagcgt gctttcacgt agagagcgac
    11341 cttaacatcc gtcttatagt tcgggtcgtt atggtacttc aactcagccg caaggtgctc
    11401 attccctctc gtcgcataca catccacctc atgcgacacg catttcccgg gtattatctt
    11461 gcgccactcc acattccagc cttctttctc aaataacgcg cttacaaagt cttcaaatgg
    11521 gtgtcccgaa ggcccaagct cgaacaatgc acgacgcaag ctataccgag cggcaacggc
    11581 acgctcctcg tcgcgcaaaa gcgcgaacgc acgacgatat atctcatggg tatcaatgcc
    11641 cgagacgata gtggatgtga tgacgtgtcc gatacgttcc gctgcttgtg cactcgcacc
    11701 agaacgtcgg agcgacgcct cgagtttgga tggatcaaac ggctctgtgg tgccgtcggc
    11761 tttgagaatg tcagtcactt acgtgattat accgcctccg acgcattcag catcgcgata
    11821 tatcaccagt gattggcctg aagcgatagg ttccggcagt tcgtcggttg gggtaaaggt
    11881 atcggtagag atatggaacg tgccggacac cttcggacca tgataccgat actgcgcttc
    11941 caagggttcg ttgttagtaa ctggggtgaa ccagttagtg tgcttaattc gaatagttgc
    12001 atcctgcact accgtcatct tctctttaga aacgaccaaa agattctttg ctatgtcctt
    12061 gcggaccacg taccaaggtc caggtgcggc atcagtgagc gccactcgtt ctccgagcgt
    12121 acgtaagacc gcgccatcat gagtaccgat ggtctttccc tccctatcca ctgcgcttcc
    12181 tggctcagga tggaattcat cgcgaaggaa gtcatctacg gacaccgcac caaggaaaca
    12241 gataccttga ctgtcttttt tctcagccac cggaaggtta aaacgcttcg ctagcgtgcg
    12301 tatctcgctc ttcttatacg taccaagcgg aaagagcgtc ttttcaagcg cttcctttgc
    12361 gactgcccac aaaaaatagc tctgatcttt ctcaggatcc acaccgcgat atacatgtcc
    12421 gtcctttgct atggcatagt gcccggtcgc tattgcatca gctccctgct ccatggcaaa
    12481 ggaatagaac gctccgaact tcacgtcgcg attgcacata atgtcagggt tcggggttct
    12541 tccctggcta tattcactca aaaggtagtc gatgaccgat ttcttgtatg cagctgaggc
    12601 atcaagcgta tgaaaaggga tgaagagtcg ggcagcgacg cgcatagcat cgcgtctatc
    12661 ggccgcccag gtgcaggtaa ggttcggtgg ataccagcct ttaatgaata cgccgacgac
    12721 gtccgctccc gcctctttga gaagagctgc agttacggca gagtcgacgc ctcccgagag
    12781 acccacgaat atacgctttc ctttcacgtt catgcgcctg ttatagcacg cacggtgtta
    12841 tcgaagatac tgctccttat ttttcttatg tccctcgaaa tcttccgcat agtgcactac
    12901 tccttctgca tcacttaagt aatacaggta atctgatgca ataggatgca gagccgcttg
    12961 aagcgcgtct ttgccggggt tgccgatagg ccctggcggc aagtcagggt attcataggt
    13021 gttatacgga gagtctattt ccaagtcatc gcccgatgga tgatacgtat cgacgccctt
    13081 tatatacccg aagaccgcat caacctggag cgccatgccg atatcaatac gcttccacaa
    13141 gatgcccgac acgacctgac gatcttcacc gactttcgtc tctttctcga gaattgaagc
    13201 catgatgacg atgcgttctt ttggttcagg ggtatctact gctatctcgc tccagacctc
    13261 gtcaaaacgg gcacgcatgc gggtaattac ttcttctggc gtcacgtcag catagaacgc
    13321 gtaggtgtca ggaaataagt atccttccag aggtgttgcg ttcgtaacaa atgtgtcggt
    13381 agcaaatccc ggaagcgatg tgccgagaat ctcacccatc tcgcgtaccg ttatgccctc
    13441 ggggaaggtg atccgcaccg cgcgtatgcc gctatctccc gtggcaaggc gatagaggac
    13501 taccgccacg ccttgtggct cagtgaaaag atatcggcct gcctttaccg acgtatcgac
    13561 tcccgtaacg cgagcaagta ctttaaagag caccgcattc tctacgatat ctcgattctc
    13621 aagctgtgtt gcggtttgag aaaacgtcat gtcttcattc acttcaacca gagcaccatg
    13681 cggaaatgag gaagcaggaa tcaaaagtat ccatgccgga agcatgacga agagaacgaa
    13741 ggcgagtcct gcatagaagc gacccttgcg gacgatatgg agatacaacg cctccagtac
    13801 gaatggaaga tacgagaaca gtaccatact agagcggaag gtttggcggc ggcgccgacg
    13861 tcgtcgagcc gatacgtgcg aggcctggag cctttgaggt cgaggatggc acatgataaa
    13921 tggtagcgat tgtttcggta gacataatca tccacggacc gcgatacggc ggatggaagt
    13981 aagaacgggc acggtatgcc tcaagcacct catgcatttc tgcacgctga cggtgaccac
    14041 caatgtcttc ccaaggatag tcattgaact tctcactcca caacggcgct gggtttatct
    14101 tgttgtactg ctgcgagatg aacaccttga acatgttcgc cgtgaaggcg ttcatagtgc
    14161 cgtgatactt atccgccggt gctgagtaga tagaacgaat acccacatca aatgcaggtt
    14221 tatcgatatt acgttcaacg gcaccaatga gttctttctg gaactcgttt gggtttggga
    14281 agccttcgac ttcttgaacg tggccttctg cgtcaacggt ttgtcgacga ttaacggtct
    14341 ttgcgcgcag cgcttctatg acttcccgac ccctttcacg ccatgagtag ggtttcccat
    14401 tgtctggatt tatccgtcca cgatactttt ctcctttggt ctcgcgaatg atgatttgag
    14461 tccagagctg ttctccgggt ccgagcgaac caaagagctc aagaatctgt gcgaaaggat
    14521 cagagatctc ttccggctta tcaccaggct tatcaagacc aaggtctaca taggtacgaa
    14581 tcgggaacgg atcatcgaga ccgtacgcat attcgcaggc aaacatctga tatggctcgt
    14641 gcagagggtc acgcagtcgc gcgtagtcca ccgcttcgat gagttcaatt tcagggtact
    14701 gcgcgtacat gtagctctca agtgcccgac gcatgttctt gcgcgtgttg atgtagaaat
    14761 gaatgttgcc ttctatcgaa gcaagctcga gcgaccacca ggggcgcgta cgaccccacc
    14821 agtacttctt gagccacgta cccgttcccg ggcctatgtt aagagacgcg aggacggtct
    14881 ccattgcacg cggcgtcttg tttatctcac gcgggatgcg catctcgagc agcacgaatg
    14941 gattcttata attgaaattc atctgacgca tgttcatgaa ccgcatcaac gcaaagcgtc
    15001 cgagaacgat aggaagccaa atggggccaa gcgtgaggag catatcccag ttctgcgcgc
    15061 tttgaactgg cgcttgcgcg gcaaacacac cagcacaaat gaaatagacc agtataagga
    15121 gccagccggg gacgatgaat ccgccgcttt cgtttgagga ctccagcatc tcatggagtg
    15181 catgaagccc ggggagctcg agctcaccgg tactgtgtcc gccgccgcct gatgacatag
    15241 ggaaagtata ccgtacgtat ataacgaatt gcgccccacc aggtggtggg gcgccttatt
    15301 ccgaaattgc ttccga
//
LOCUS       DAORIN010000005        13792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_746_length_13792_cov_5.776880, whole genome shotgun sequence.
ACCESSION   DAORIN010000005 DAORIN010000000
VERSION     DAORIN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13792
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_13792_cov_5.776880"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="PK109_00440"
     CDS             complement(<1..1148)
                     /locus_tag="PK109_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRH24047.1"
                     /translation="MIGKRRRSSKDWELAPDEIFLDSSNLPDFDAARLEGRLERPVSR
                     TTFFGLGGGVALLILVLIIQAVNLELVKGSAYAAQSERNRLRPEVIFAERGAITDRNG
                     TPLAWNEAGEEGFPERVFASPGYGHILGYVSYPEKDKSGNYYETEIEGVAGIERAFNG
                     MLAGVNGTLLVEEDARGTIESQGSIVAAKNGETLMLSIDARVQEAFAGAIKELADKIP
                     FKGGSGILMDVETGEILALVSYPEYDPNVLSSGKDHGTIVSYNNDSREPYLDRAVSGL
                     YTPGSIVKPMIAAGAFNDGIISAEKAIVSTGSISLPNPYDPSKPSIFKDWKAHGATDM
                     RHAIAVSSDVYFYTVGGGFGDQKGLGIDRLNYWYKAFGFLSETGIEIE"
     gene            complement(1145..1399)
                     /locus_tag="PK109_00445"
     CDS             complement(1145..1399)
                     /locus_tag="PK109_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24048.1"
                     /translation="MMKGVLVVAALLSPFLFPFAVTLVLVAAASIVFPPIALISGIVT
                     DLAYYTPAASFLPVASISGLLISVVALFVRRFLKARIIGG"
     gene            complement(1396..2145)
                     /locus_tag="PK109_00450"
     CDS             complement(1396..2145)
                     /locus_tag="PK109_00450"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRH24049.1"
                     /translation="MKTQYFRRPKSFLSPQQSIVAIGAGVIILILIVLRLALPGVFTA
                     LFTPLWNLGNFFTGQAHRTEDAATLEARVLALEAENETLRNENRALTENSDDETRGIV
                     AGVVARPPLSPYDVLILGEGTDQGLYEGMQAFSKGIPVGTLESVSAGSARVALYSSSG
                     RATEGWVGEARVPITLYGLGGGAFQADVPRESGVVEGDMVYIPGPGALPMGVVVAIES
                     NASSPRSLVRIRPMANPFTMTSVSVVAAPSL"
     gene            complement(2117..3178)
                     /locus_tag="PK109_00455"
     CDS             complement(2117..3178)
                     /locus_tag="PK109_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRH24050.1"
                     /translation="MAKEKRISKNSLFSSDIGIDLGTANTLVYVRGRGVVIAEPSVVT
                     VNDKTGQVVAIGREAKVMLGKTPPHVRAVRPLSHGVISDFEVTEELLAYLIRKAQGDR
                     ARLFGPRVVVGVPTGVTNVESRAVRDAALASGAREVFIVEETMAGAIGAKLPVTEPVG
                     TMVLDIGGGTTDIAVIALGGTVASKSSQVAGDRFNENIIDFVRSEFQLAIGEKTAEDV
                     KIGIGAVLPLSAPLSQAVRGRDLATGLPREITLTDAHIRDALQPSLDKLMEAMKEVIE
                     STPPELLSDVLERGVTVFGGGALLRGLPELLSKMLGVPVYVATDPLTAVVRGTGIITE
                     DPRIWGGIFLNEDAILPQA"
     gene            complement(3180..4421)
                     /locus_tag="PK109_00460"
     CDS             complement(3180..4421)
                     /locus_tag="PK109_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRH24051.1"
                     /translation="MRQSKLFTKTRREAPSDESAKNAQLLIRAGYVHKEMAGVYSYLP
                     LGLRTLNRIVQIIREEMDAIGGQELVMTALQDKDLWSRTDRWDDDKVDNWFKTEFKSG
                     GQTGLAITHEEPLTRIMTDHVSSYRDLPLKAYQFQNKFRNEMRAKSGVMRGKEFLMKD
                     LYTFSKDEAEHAAAYAEVRAAYIKVFARLGLGAVTYPTYASGGIFSPFSEEFQTLTDA
                     GEDTIYVHEGKGIAVNQEVYTDETLASLGLSKDELVEKKSVEVGNIFTLGTRFSEPLG
                     LTYTDTTGARLPVMMGSYGIGPTRVLGTIVEILSDDKGIVWPESVAPFRFHLLNLMTN
                     DADATSFADALYNDLSKKSEVLYDDRDARAGEKFADSDLIGIPWRVNVGKETVATGMV
                     ELVNRATGEMKKVTREELLAL"
     gene            complement(4519..4662)
                     /locus_tag="PK109_00465"
     CDS             complement(4519..4662)
                     /locus_tag="PK109_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24052.1"
                     /translation="MTGAPSDVRPSQHVEVELNNCRSDVQEYWRVTVAEAIARVNESE
                     ATQ"
     gene            complement(4937..6037)
                     /locus_tag="PK109_00470"
     CDS             complement(4937..6037)
                     /locus_tag="PK109_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRH24053.1"
                     /translation="MTIIIFILVLVALIVVHELGHFFAAKWAGMRVDEFGIGYPPRAW
                     TFWKHNGTEYTLNWLPFGGFVKIFGEDESAEGGVKATDSFVSKPHYVQALVLIAGIAM
                     NIVFAWALLSLTLAIGLPRGLSDAEIPLAEDAALAVARVVADSPAADAGLKEGDIIRS
                     ASYDTTTFTGADADAFTAFIAESDGEDVVLSVLRAGEPVTLTAHPETLIVPTDPDRLA
                     LGIGIGAVGTVTTPWWQAPIEGAVLTWNVTQQVAVGLIHFFGGIFTFTADLSQVSGPI
                     GIAGAVGTASDTSLAALLSLMAVISINLALINIIPVPALDGGRLLFVIIEAITRKPIP
                     NGVANALNTGGFALLILLMLVVTASDLWKAFV"
     gene            complement(6034..6471)
                     /locus_tag="PK109_00475"
     CDS             complement(6034..6471)
                     /locus_tag="PK109_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24054.1"
                     /translation="MHEPGGNFESGGEDKARQDAHNIHESEFLLVSEVFERGDRIRIW
                     QQGIHSHESIVAQAYGGTIECEIHQDVDPNPLEGKIVKLLTNINVDGEDSWTTVKHEK
                     PKWAGSKLYKAVMTDGTVVYIEKYPKGIEISENRDKQSQGLHL"
     gene            complement(6477..7028)
                     /gene="frr"
                     /locus_tag="PK109_00480"
     CDS             complement(6477..7028)
                     /gene="frr"
                     /locus_tag="PK109_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRH24055.1"
                     /translation="MAYDFSKLKADIKEAEEWLSREFSTIRTGRASAVLLDGVKPEAY
                     GTRTPLREIGSVTVEDARTIRIVPWDKTLGKAVEKAIVEADLGVSVVSDDQGLRVLFP
                     ELTAERRTMLQKVAGDKLEQARVTLRGHRTDALKALDAAEKEGGMGEDELKRLKEEVQ
                     KHIDAGNAALDAALKKKQDEIAS"
     gene            complement(7045..9573)
                     /locus_tag="PK109_00485"
     CDS             complement(7045..9573)
                     /locus_tag="PK109_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRH24056.1"
                     /translation="MKTEGGAFNPHEQRPIRRMRVTVLTDEEGRRAGERLLKVCEVGK
                     EPVLIEVDDPQLYPAGSTIEFDWKSVTLRKNFTNNIAIYIGKVVEVISPANDDTEPSG
                     ELIDYSRPWPEEWFGKLEKSSTQEHSKNREVEALSRLEEERPVSEDKEKHKEFYPVEY
                     SKGGFFARVGTERRYIANADELLLLPGDEIVLAPSEGQYEVVAAEVAYEGRSTVLGRL
                     EYRDGTTTPELRFVVRAFNADGTLDKFRFSIDPVVKGMSEQQIQELVENRSYIVFDRN
                     TMQVTGEPSPEDTMSLYEAAELSVLTNLGIEQHNKNAEMLRRGEREQVNALMRAYGVP
                     KRAAGKAISRASAQEMEAKITHEIGIGGIEDFRNGERITLAVDARGTGIRDDAFTIGP
                     SSRGEGWHEIGIHIADPRLLVPAGSALDRGASERSASIFIPGRKILMLPEAVTALGSL
                     DEGVDRRAFALIFDAKLGPDGEVEIRGIRFAPTAIQVGKTFDHDEADALLDGEGESNE
                     PYAKAWNTFGQVMDALNQRKLIRKNSERQPHALEDSDEIVATANMLFNRASAEAFTRL
                     KRSGNPQMKRLQEMALGTYRNAVSKAGEPPVIEQSTTPRGNLYYGGQYAYLSSPLRDY
                     SSIIGLRSIAFLAELSKREREGEELTPETAHLLAERYAVDVVGIQLSDETRALSGDDF
                     IAAVSEEAREHLDDVLKHALAREELIRRTERAMKGMKTLDNLGAEMQKEGPINLPKGI
                     QVEFTDRRNKPSVEDGVTWVYGYAKVSRKKNQFLHKFSPSRSDTLRLGVKFPLPEGMQ
                     FPTAPSDLVLDGRITGVDVEKGRLILDIRKGRRAPVAPTYSRRR"
     gene            complement(9584..9853)
                     /locus_tag="PK109_00490"
     CDS             complement(9584..9853)
                     /locus_tag="PK109_00490"
                     /inference="COORDINATES: protein motif:HMM:NF039821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HRH24057.1"
                     /translation="MTTSPAFLSIVLPPGAASLVPNLETALLDTIALAVVEALPEEQL
                     SAFEEVLAEGDEKKIVAFIEANVPDAREVLYPIVTDMAERVAALV"
     gene            complement(9855..10844)
                     /locus_tag="PK109_00495"
     CDS             complement(9855..10844)
                     /locus_tag="PK109_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24058.1"
                     /translation="MNGTSSEEPPVPQKLRVEASDDYVVHDEFSDDPFNYEDNVPTAR
                     TREILAEKEHTSTSAADLLNEALRSSPFARMEDLVDNYQKTMVLGVRNAYFVTSFNFW
                     NKQASVADLYKANVEYEKALPRVKRELDRIKGISEEDRAHLLRSFVVDDVERLNVIHE
                     ALMSEKKQASAERIRKTIAKMSAGVRRVATAALVLFVASSAEVPSSPTLPTYRAPVIG
                     KVTPERRVVAEATARGANHMFYRFFRTMRYTDPKSYERLAGNENPLAISERFAQLTGF
                     WKMNEGENRSAMLQDGDRIELTPQGEVVFIEAGGVRHELTQNGQPVSQDWPQF"
     gene            complement(10932..11690)
                     /locus_tag="PK109_00500"
     CDS             complement(10932..11690)
                     /locus_tag="PK109_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24059.1"
                     /translation="MAQFNMCALNGETGEIRDGINFPELLSNELNRSFEVQGPPAISR
                     GILVGAQHRPLVIEDKQVIVPRNRGQVAWQGESSPITRANKRGLVQAASGSVQTIRTE
                     AGGTRHLIVAPVEGEKAVLVIVQTGLPGNARQQEAALLEMLSKGERDWEHGAFSVDSG
                     ARGLVRKIRTAKTRSGGVLTRTNHLIEVKPGGSILVAGMLNENAIWRFSNEGGRLRRV
                     DAGENPRATFARLRREQRGRRDDVEDEMPLAAAS"
     gene            complement(11802..12482)
                     /locus_tag="PK109_00505"
     CDS             complement(11802..12482)
                     /locus_tag="PK109_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24060.1"
                     /translation="MNHHAFYVVGDTEEGVQRALEYGARVLHLEAAGNPDVVVLRYGL
                     FPVEEARKITELVSRVPSRGDKKLIVIAATRLFHESQNALLKVFEEPSEGTTIVLIVP
                     SDGVIIPTLRSRLIKLPESGEGEAVPESVHAFLKGSAEEREKLIGKLLDRAKSDKPEE
                     KQAARTDALALANGLAKAVYTKRGDPSVRAFLSDLDHFIPILHERSAPLKAIFEHLLI
                     TFPRNIDK"
     gene            complement(12487..13500)
                     /locus_tag="PK109_00510"
     CDS             complement(12487..13500)
                     /locus_tag="PK109_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRH24061.1"
                     /translation="MAFFSPKLGIDLGTTNVLVFLPGKGVILNEPSVVAVSTENNKVL
                     AIGVEAKKMIGRTPDSIIAYRPMKDGVIADYRVTEAMLRYFMRKALGKWNFFKPTVLV
                     SVPAGVTSTEKRAVIEAAVKAGAADAFVVKEPILAAIGAGIPIQEPSGRMVADLGGGT
                     IDVAVISLGGIVASTSVKVAGNALDRAIVDYVKKQYNLSIGDKTAEEIKITIGSAVPV
                     SEDLVMPVKGRDLVSGLPRTVELKTNEIVTACNRELREMMNAIRKVLQETPPELASDI
                     IDNGIILTGGTSQLRNMPELVFRRTGVEAKLATDAYYCVARGTGVALKHLDTYKKSII
                     SKR"
     gene            13577..>13792
                     /locus_tag="PK109_00515"
     CDS             13577..>13792
                     /locus_tag="PK109_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRH24062.1"
                     /translation="MPKDDLSFIVNGGKTFSGSILTNRSKNGAVSLLAASLLNKGTTT
                     LHNVPKIEEVNRLLEVLSSIGVEAVWDD"
BASE COUNT         3179 a         3813 c         3367 g         3433 t
ORIGIN      
        1 ttctcaattt caatacctgt ttctgataga aacccgaacg ctttgtacca atagttgagg
       61 cgatcaatac cgagtccttt ctggtccccg aacccgccac ccaccgtata gaaatagacg
      121 tctgaggata ccgcaatggc gtgacgcatg tcggtagcgc cgtgcgcttt ccagtctttg
      181 aatatagaag gctttgaagg atcgtatggg ttcgggagtg aaatggatcc cgttgagact
      241 atcgccttct ctgcggagat aatgccgtca ttaaacgcac ccgccgcaat cattggcttc
      301 acgatagacc cgggagtata gagaccggag acggcgcgat ccaagtacgg ctcacgcgaa
      361 tcattgttat aggacacaat agtgccgtga tcttttcctg aagagagtac gttcgggtca
      421 tactcggggt atgacacgag cgcgagtatc tcacccgtct ctacatccat gagaatgcct
      481 gagccgcctt tgaacggaat cttgtccgca agttccttga tggcgcccgc aaacgcttcc
      541 tgcacacgtg catcaatgga gagcatgagg gtctctccgt tttttgcagc gacaatggac
      601 ccttgcgact ctatggtgcc gcgtgcgtct tcttcgacta agagagtacc gtttacgccc
      661 gcgagcatgc cattgaacgc acgctcaatt cccgccactc cttcaatctc agtctcatag
      721 tagttaccgg atttatcttt ctctggataa gagacatacc cgagaatatg accgtagccg
      781 ggagaggcga acacacgctc ggggaatcct tcttctccag cctcattcca cgcaagcggc
      841 gtcccgttac ggtcagtgat tgccccccgt tcagcgaata tcacttcagg acgcaagcga
      901 ttacgctcgc tctgcgcagc atacgctgaa cccttcacca attctaggtt tacggcttga
      961 ataataagca cgaggatgag aagcgcgacg ccacccccaa gaccaaagaa ggtagtacgc
     1021 gatacaggac gctcaagacg tccttcaagc cgtgcagcat caaagtcagg aaggtttgag
     1081 gaatcaagga atatctcatc aggagcgagc tcccaatcct tactacttcg ccgtcgcttc
     1141 cctatcatcc ccctataata cgtgctttaa gaaaacggcg cacgaagagc gctacgactg
     1201 atatcagaag ccctgaaatg cttgctacgg gaagaaacga ggcagcgggg gtgtagtagg
     1261 caaggtccgt aactatcccg ctgatgaggg ctataggcgg aaacacaatg cttgccgctg
     1321 ccacaagcac gagtgttacc gcaaacggaa acaaaaaagg tgaaaggagc gccgctacga
     1381 ccagtacccc tttcatcata gggacggagc agcgactacg ctcacactcg tcatcgtgaa
     1441 cgggtttgcc ataggacgaa tgcgcactaa ggagcgcggc gacgatgcgt tgctttcaat
     1501 cgctacgacc actcccatcg gaagcgcgcc ggggccgggg atatatacca tgtctccctc
     1561 aaccacgcct gactcacgcg gcacatcagc ttgaaacgca cccccaccaa gaccgtagag
     1621 ggtaataggt acacgagcct cccctaccca tccttccgtt gcgcgaccac ttgaagagta
     1681 gagagcaaca cgagcgcttc cagcgcttac gctttcaaga gtcccaaccg gtattccttt
     1741 tgaaaaagcc tgcatgcctt cgtatagtcc ttgatcggtt ccttcgccaa ggataagtac
     1801 atcgtacgga gacagcgggg ggcgggctac tacgccggca acaattcctc ttgtttcgtc
     1861 gtcactattt tccgtgagtg cacgattctc gttacgaagc gtctcgttct ctgcttcaag
     1921 ggcgagcact cgtgcttcaa gcgttgctgc gtcttcagta cgatgcgcct gtccggtaaa
     1981 aaagttccca agattccata ggggcgtaaa gagcgccgta aacactcctg gaagtgcgag
     2041 gcgcagcacg atgagtataa gtatgatgac accggctcct atagcaacga tgctctgttg
     2101 cggagacagg aacgatttag gcctgcggaa gtattgcgtc ttcattgagg aatatgccac
     2161 cccagattct tgggtcttcg gtaatgatgc ccgtaccgcg cacgacagcg gtcaacgggt
     2221 ccgtcgctac atacaccggc acgccgagca tctttgaaag aagttcagga aggccacgca
     2281 ggagcgcgcc accaccaaac accgtgacgc cacgttcaag cacatccgag agtagctcgg
     2341 gcggggttga ttcaatcacc tccttcattg cctccatcaa cttatccaac gatggctgga
     2401 gcgcgtcacg aatatgagca tcggtgagtg taatctcacg aggaagaccc gtggcaagat
     2461 cgcgtccacg tactgcctgc gagagcggtg cggaaagcgg taacacggca ccaatgccaa
     2521 tcttcacatc ttctgcggtc ttctcaccaa tagcgagttg gaattcgctt cgtacaaaat
     2581 caatgatgtt ctcgttgaac cggtcccctg ctacctgaga gctctttgaa gcaacggtgc
     2641 cgccaagcgc gatgaccgca atgtcagtgg tgccgcctcc gatgtcgagt accatagtgc
     2701 cgacaggctc ggtgaccgga agctttgcgc caatggcgcc tgccatggtt tcttcaacga
     2761 taaatacttc acgagcgcct gacgcgagcg ctgcgtcgcg taccgctctt gattcaacgt
     2821 tggttacgcc ggtagggact cccacaacaa cgcgcgggcc aaacaagcgt gctcgatctc
     2881 cctgtgcttt tcgtatcaaa taggccaaca actcttctgt tacttcaaag tcagaaataa
     2941 caccatggga gagtggacgc accgcacgca cgtgtggcgg cgtcttacca agcatcacct
     3001 ttgcttcgcg tccaatagcg accacctgcc ccgtcttgtc attcacggtc accacagaag
     3061 gttcggcaat aacgacgccg cgcccgcgca catacacgag tgtgttcgcc gttcccaagt
     3121 cgataccaat atcgctggag aagagagagt tcttcgagat tcgcttttct tttgccatat
     3181 tagagcgcaa gtaattcttc gcgggtcacc ttcttcattt ctcccgttgc acggttcacg
     3241 agttcaacca tacccgtagc caccgtctcc tttccgacgt tcacccgcca tggaataccg
     3301 ataaggtcgc tgtcagcgaa cttctctccc gcgcgtgcat cgcgatcgtc atacagaacc
     3361 tcgctcttct tactaaggtc gttgtagagc gcatcggcaa acgaagtcgc gtccgcgtca
     3421 ttagtcataa gattcaaaag atggaaacgg aatggcgcaa cactttctgg ccagacgata
     3481 cctttgtcgt ctgaaagaat ttcaacgatg gttcccaata cgcgtgttgg gccaatgccg
     3541 taagaaccca tcatcaccgg aagacgagcg cccgttgtgt cagtgtacgt aagcccaagt
     3601 ggttcagaga agcgcgtgcc gagcgtaaat atatttccta cttctaccga tttcttttca
     3661 accaattcgt ccttagatag accaagtgac gcgagcgttt catctgtata cacctcttga
     3721 tttaccgcta ttcccttacc ttcatggaca tagatggtgt cttcaccagc atcagtaagt
     3781 gtttgaaatt cttcggagaa tggactaaag atgccacctg atgcgtacgt tgggtacgtg
     3841 acggcgccga gaccaagacg agcgaatact tttatatatg ccgcacgcac ttcagcatac
     3901 gcagcagcgt gctcagcctc atcttttgag aaggtgtaga gatctttcat gaggaactct
     3961 tttccgcgca tcacgcctga cttggcgcgc atctcattgc ggaacttgtt ctgaaactga
     4021 tacgccttga gtggaagatc acgatacgac gacacatggt cggtcatgat acgcgtcagc
     4081 ggttcctcgt gcgtaatagc aaggccggtc tgtccaccgc ttttgaattc agtcttgaac
     4141 caattatcaa ccttatcgtc atcccatcgg tcagtacggc tccaaagatc tttgtcttga
     4201 agcgcggtca ttaccagttc ttgtcctcct atcgcatcca tctcttcacg gattatttga
     4261 acgattctgt tgagagtccg tagtccaagt gggagatacg aatacacacc tgccatctcc
     4321 ttgtggacgt agccggcgcg aatgaggagc tgcgcgtttt tcgcagattc gtctgacggc
     4381 gcctctcggc gcgttttcgt gaaaagctta ctttgccgca ttacccggac agtatagccc
     4441 cgggaccctt agggggcaaa agcaaactgg cctccccttt cgaggaagcc agtccggact
     4501 ggtcggcgcg gtgaacgcct actgggtcgc ttcagattcg ttcacgcgag ctatcgcctc
     4561 agcgacggtc acacgccagt attcctgcac atcactgcga cagttgttca actcgacttc
     4621 aacatgctgc gagggacgca catcgcttgg cgctccggtc acctgagtat ccggaaacag
     4681 ccaggcaacg tactcctgac accacgcgtc agcctctggg gagagggcaa cggcggtgcg
     4741 cggcggtgct tcaggttgtc gggttgtctg ggcgtgcgcc ggctgcgtag agacaagtgc
     4801 cagcgcgaac agaatttttt gaaaggtctt caagggtgtt cccctctcta cggagcatca
     4861 ttgctccgta tctctgcgtt ttttaggtgc agagtcaccg tgaacgtatt atatctaaat
     4921 atacctattt gtcaaactag acgaacgctt tccacagatc agaagcagtg accacgagca
     4981 tgaggaggat aaggagcgcg aacccgccgg tgttaagcgc gtttgcgacc ccgttaggaa
     5041 ttggtttgcg ggttatcgct tcgataatca caaacaagag acgtccccca tcaagcgccg
     5101 gtaccggaat gatgttgatg agcgcgaggt taatggagat gaccgccatc aaagaaagga
     5161 gtgcggcaag gcttgtgtct gacgcagtgc ccactgctcc cgctatacca atagggcccg
     5221 atacttgaga gaggtctgca gtaaaggtga aaatgccgcc gaagaaatga ataaggccga
     5281 cagcaacctg ctgggtgacg ttccaggtga gcacggctcc ttcaatgggt gcctgccacc
     5341 acggcgtcgt tacggttcct actgctccta tcccaatacc aagcgccaaa cgatcaggat
     5401 cggtagggac gataagggtt tcagggtgtg cagtgagagt gactggttct ccagcgcgta
     5461 gaacggaaag taccacgtct tccccgtcgc tttctgcgat aaacgcggtg aaggcgtcag
     5521 cgtctgcacc ggtaaaggta gtagtgtcgt aggaagcgct tcgaatgatg tccccttctt
     5581 taagacctgc gtctgctgcg ggagaatctg ccaccactcg cgctaccgca agtgctgcgt
     5641 cctcggctaa tggaatctca gcatcagaaa gaccgcgtgg taggcctatg gcgagcgtga
     5701 gcgaaaggag tgcccatgcg aatactatgt tcatcgcaat gcctgcaatg aggacgagcg
     5761 cttgcacgta gtgcggcttt gatacgaatg agtccgtcgc ctttactcct ccttcagcgc
     5821 tctcatcttc accgaagatt ttcacaaacc cgccaaacgg cagccagttc aaggtgtact
     5881 cagtcccgtt atgcttccaa aaggtccacg cacgtggcgg gtacccgata ccaaattcat
     5941 ccacgcgcat acccgcccac ttagcagcga agaagtggcc aagctcatgc accacaatga
     6001 gtgcgacgag taccagtatg aaaataatga tggtcataga tgcaggcctt ggctctgttt
     6061 atcgcgattc tcgctaatct ctattccctt cggatacttc tctatatata ccaccgtgcc
     6121 gtcagtcata acggctttgt atagttttga ccctgcccac ttcggtttct cgtgcttcac
     6181 ggtagtccag ctatcttcac catcgacatt gatgttcgtg aggagtttaa cgattttgcc
     6241 ttcgagcggg tttggatcga cgtcttgatg tatttcacac tcaatggtgc cgccatacgc
     6301 ttgagcaaca attgattcat gcgagtgaat accctgttgc catatgcgaa tgcggtcacc
     6361 tcgttcaaat acttcggaca cgagaaggaa ctcactctcg tggatgttgt gagcgtcctg
     6421 gcgcgcttta tcttccccac cactctcaaa gtttcccccg ggctcgtgca tatcgattag
     6481 ctcgctatct cgtcctgctt cttcttgagc gccgcatcaa gtgctgcgtt tccggcatca
     6541 atatgctttt gcacttcttc cttgagacgc ttcagttcat cttctcccat acccccttct
     6601 ttctcagcag catccagggc cttgagtgcg tcagtgcggt gcccacgaag cgtcacgcgt
     6661 gcctgttcca acttatcgcc ggcaactttc tggagcatgg tgcggcgctc ggccgtgagt
     6721 tcggggaaaa ggacacggag tccctgatcg tctgaaacga cggagacacc aagatctgct
     6781 tcaacgatag ccttctcaac ggctttcccc agcgtcttat cccacggcac gatgcgtata
     6841 gtacgagcgt cttctaccgt tacggaacca atctcacgca acggcgtgcg cgtaccgtat
     6901 gcttccggct tcaccccatc taaaaggaca gcagatgcgc gtccagtacg aatggttgaa
     6961 aactctcgag agagccattc ttctgcttct ttaatatctg cctttagttt agaaaagtcg
     7021 tatgccatag atacgggcag tatatcatcg gcgacgtgag tatgtaggag caaccggcgc
     7081 acgccttcct ttacgaatat caagaataag acgtcccttc tctacgtcga ctcccgtgat
     7141 gcgaccatca agcacaagat cagaaggagc tgtcgggaac tgcatgcctt caggaagagg
     7201 aaacttaacc cctagacgta aagtatcact gcgagatggt gagaatttat ggaggaattg
     7261 attcttttta cgacttacct ttgcgtaacc atatacccat gtgaccccgt cttcgacaga
     7321 gggcttgttc cttcgatccg taaactctac ttgaataccc tttggtagat taattggtcc
     7381 ttctttctgc atttcggcac cgagattatc aagtgttttc atgcctttca tggcgcgttc
     7441 agtacgtcgt atgagctctt cgcgtgcaag tgcatgtttc aacacatcat caaggtgctc
     7501 gcgtgcctct tctgaaactg ctgctatgaa atcatcacct gacagtgcac gtgtctcatc
     7561 tgaaagttgg attccaacga catcaaccgc atatcgctct gcaagcaaat gagcagtctc
     7621 tggcgttagc tcttcccctt ctcgttcgcg tttggacaat tctgcgagaa acgcaattga
     7681 acgcaaccca ataatacttg agtaatctcg aagcggcgaa gaaagatacg catattgtcc
     7741 tccgtaatac agattgcctc ggggagtagt gctttgctcg ataacgggag gctcgcctgc
     7801 ttttgaaact gcattgcggt acgtgcctag cgccatttct tgaagacgtt tcatctgagg
     7861 attaccactt ctcttgagtc gagtaaatgc ttcagctgaa gctcgattga aaagcatgtt
     7921 cgcagtagct actatttcgt ctgaatcttc aagtgcatga ggttggcgct cagaattctt
     7981 acgaatgagc ttacgttgat tcagtgcatc cataacctga ccaaacgtat tccatgcttt
     8041 tgcgtatggc tcgttcgact ctccctctcc atcaagaaga gcatcggctt cgtcatgatc
     8101 gaatgtctta ccaacttgta tcgcggtggg cgcaaatcgt atacctcgta tttctacttc
     8161 cccgtcgggc ccaagtttcg catcaaaaat gagtgcaaaa gcacgtcgat ctaccccttc
     8221 atccaaactg ccaagcgccg taaccgcttc cggaagcata agtatcttgc gtccagggat
     8281 gaatatactg gcgctacgct cagacgctcc tctatcgagt gcgcttcctg caggtacgag
     8341 gagacgggga tctgcaatat gaatacctat ctcgtgccat ccttcaccac gtgatgacgg
     8401 accgatagta aaggcatcgt cgcgtattcc tgtaccgcgt gcatcaaccg cgagcgtaat
     8461 acgttctcca tttcgaaagt cttcaatacc cccaatgcca atctcgtgag taattttcgc
     8521 ttccatttct tgtgcgcttg ctctacttat cgcttttcct gcagcacgtt tcgggactcc
     8581 atacgcacgc atgagagcat taacctgttc acgctctcca cgtcgtaaca tttctgcatt
     8641 tttattatgc tgctcaatac caaggttcgt gagcacgctg agctcagctg cctcatataa
     8701 gctcatggtg tcctccggac taggctcgcc cgttacttgc atggtgttac ggtcaaaaac
     8761 aatataagag cgattttcaa ccagctcttg tatctgctgt tcactcatcc ctttcacaac
     8821 aggatctatc gaaaatctga atttatcgag cgtgccatct gcattaaatg cgcgcactac
     8881 aaaacggagc tctggagtgg tggtaccgtc tctatactcg agacgaccaa gaacagtcga
     8941 acgtccttca taagcgactt ctgcagcaac tacttcgtat tgcccttcgc tgggtgcgag
     9001 cacaatctca tcgcctggca gcaggagaag ctcgtctgca tttgcaatat accggcgctc
     9061 agtaccaaca cgtgcaaaga atccaccttt tgaatactca acgggataaa attctttgtg
     9121 tttttctttg tcttccgata ctggacgctc ttcttcaaga cgactaagtg cttcaacttc
     9181 tcggtttttt gaatgttcct gagtggaaga cttttcaagt tttccaaacc actcttctgg
     9241 ccaaggacga gagtagtcta tcaattcacc actaggctcc gtatcatcat ttgctggtga
     9301 tatgacttca actactttcc caatatatat tgcaatattg ttagtaaagt tttttcggag
     9361 tgtcaccgat ttccaatcaa actcgattgt cgatcctgca gggtacaact gcggatcatc
     9421 aacttcgata agtacaggtt ccttgcccac ctcacacact ttaagaaggc gttcaccggc
     9481 gcgacgtcct tcttcatctg tcaggaccgt gactcgcatc cttcgaatcg gacgctgctc
     9541 gtgaggatta aatgcgccgc cttccgtttt cataccttct ctattatacg agcgctgcaa
     9601 cgcgctcggc catgtccgtc actatcggat agagcacttc gcgtgcgtcg gggacatttg
     9661 cttctataaa agcgactatc ttcttttcgt ccccttcagc aagaacttct tcgaaggcac
     9721 tgagttgctc ttcaggaagc gcttcaacga cggcaagcgc tatggtgtcg agaagtgccg
     9781 tctcaaggtt aggcacgagc gacgctgcgc ctggtgggag cactatcgag aggaatgcag
     9841 gagaggtagt catattagaa ttgtggccag tcctgagaca ctggctgtcc gttctgggta
     9901 agttcgtgtc gcacaccgcc tgcttcgata aacaccactt ccccttgagg agtaagttcg
     9961 atacggtccc cgtcttgaag cattgcagaa cgattctcac cttcgttcat cttccagaag
    10021 ccagtgagtt gcgcgaatcg ttctgaaatc gccagtgggt tttcgttccc tgcaagacgt
    10081 tcatatgatt taggatctgt gtaccgcatc gtacggaaga accggtagaa catatggttt
    10141 gcaccgcgcg ccgttgcctc agcgacgacg cgtcgttctg gggtcacttt cccaatgaca
    10201 ggtgcgcggt acgtaggaag ggtcggactc gacggcacct ccgccgacga tgcgacgaag
    10261 agtacgagtg ctgcggttgc gacacggcgc acccccgcag acatcttcgc aatcgttttg
    10321 cgtatacgct ctgcacttgc ctgtttcttt tcagacataa gcgcttcatg tatgacattg
    10381 agtcgttcaa catcgtcaac gacaaacgaa cggagtaggt gtgcgcggtc ttcttcgctt
    10441 atccctttta tacgatccag ctctctttta actcgcggca atgccttctc atattcaaca
    10501 tttgctttgt agagatctgc cactgacgct tgcttattcc agaagttaaa tgaggtgaca
    10561 aagtaggcgt tacgcacccc gagaaccata gtcttctgat agttatctac gagatcttcc
    10621 atgcgcgcga aaggagatga cctaagcgct tcgttcagta agtctgccgc tgatgtagat
    10681 gtatgttctt tctcagcaag aatttctctc gtccgtgctg tcggcacatt atcttcgtag
    10741 ttaaaagggt catcggaaaa ttcgtcatgg acgacatagt cgtccgacgc ttccacacgg
    10801 agtttttgtg gcactggagg ttcttccgaa gacgttccat tcatactcaa tcgataaaat
    10861 agtagcacgt ggtaaagaaa gggcgcggtg ttaccaccgc gccccaaccc taacttcgga
    10921 tccgcgagat gctagcttgc ggcggcgaga ggcatttcat cctcaacgtc gtctcttcgg
    10981 ccccgttgtt cacggcgcag tctagcaaat gttgcacgcg ggttttcacc cgcatccact
    11041 cgacgcaacc ggcccccttc attgctgaag cgccaaatcg cgttttcgtt gagcatgcca
    11101 gccacgagga tcgacccacc gggtttcacc tcgattagat ggttggtgcg ggtaaggact
    11161 ccgccgctcc gagtttttgc agtccggatc ttgcgaacca gaccacgagc accactgtcc
    11221 acagagaaag caccgtgttc ccagtcacgc tcacctttac tgagcatttc caagagggca
    11281 gcttcctgct gtctcgcatt tcccggcagc cctgtctgga cgatgacaag gactgccttt
    11341 tccccttcaa caggggcaac aattaggtgc cgggtcccgc cagcttcggt ccggatagtc
    11401 tgcactgacc cggaagcagc ttgcacgagg ccccttttgt tcgcacgcgt gataggagag
    11461 ctttccccct gccacgctac ttgcccgcgg ttacgcggga cgatcacctg cttgtcttcg
    11521 atcacgagtg ggcgatgttg cgcgcccacc aagatccccc gagagatagc tggggggcct
    11581 tgcacctcaa aacttctgtt caactcgttg gacagaagct ctggaaagtt gatgccatca
    11641 cgaatttcgc cggtctcgcc attaagggca cacatgttga actgagccat ggttaggctc
    11701 ctttctgctg tcggtgggtt gtcaatctag tgtcttccgt acctcccaca ttggcacgga
    11761 ctagaaccat ttcaggctca gacttattat cacattattg actatttgtc aatgtttcgt
    11821 gggaacgtaa taaggagatg ctcaaagata gcttttagcg gtgccgaacg ctcatggaga
    11881 atgggtatga aatgatcgag gtcagaaagg aacgcccgca cgctcgggtc accccgcttt
    11941 gtgtagactg ctttcgcgag accgtttgca agcgcgagtg cgtcggtccg tgctgcctgc
    12001 ttctcttctg gcttatctga ctttgcacgg tcgagaagct ttcctataag cttctctcgt
    12061 tcctctgcac ttcccttcag gaaagcgtgg actgattcag gcactgcttc tccttccccg
    12121 ctttccggaa gcttaatgag acgtgaacgg agcgtcggaa tgatgacacc atcactcggg
    12181 acaatgagga cgatagtagt tccctctgat ggttcttcaa atacttttag gagtgcgttc
    12241 tgtgactcat gaaagagacg cgttgctgca ataacaatga gttttttatc tccacggctc
    12301 gggacgcggc tcacgagctc cgtaatctta cgtgcttctt ctaccggaaa gagtccgtag
    12361 cggagcacta ccacgtcagg gttacctgca gcttcaagat ggagaacgcg cgcgccatac
    12421 tcgagcgcgc gctgtactcc ttcttctgta tcgcccacta cataaaaggc gtgatgattc
    12481 attgcgctat cgctttgaga tgatgctctt cttataggta tcaagatgct tcaaggcaac
    12541 cccagtgcca cgcgcgacgc agtagtaggc gtcagtcgca agcttcgctt caacgcctgt
    12601 gcgacggaac acgagttctg gcatgttacg cagctggctt gtgcctcccg taaggatgat
    12661 gccgttatca atgatgtctg aagcaagctc tggtggcgtc tcttggagca ctttacgaat
    12721 cgcattcatc atctcacgga gctcacgatt gcacgccgtc actatctcat tcgtctttaa
    12781 ctccaccgtg cgtggaagtc ctgacacgag gtcgcgtccc tttacgggca tcaccaaatc
    12841 ttctgagacc ggtactgctg aaccaatagt tatcttaatt tcttctgccg tcttatctcc
    12901 tatagaaagg ttgtactgct tctttacgta atcaacgatg gctctatcaa gcgcgttccc
    12961 tgccaccttc actgaggttg atgccacgat tccaccaagc gagatcacgg caacgtcgat
    13021 agtgccgccc ccaaggtcag caaccatgcg tccagaaggt tcttgaatag gaatgcctgc
    13081 accaatagcg gcaagaatgg gttccttcac gacaaacgcg tcggcggctc ctgctttcac
    13141 tgccgcttca atcacggcgc gcttctcagt tgacgtaaca ccagcaggca ctgacacgag
    13201 cacggttggt ttaaagaagt tccacttccc caatgcctta cgcatgaagt agcgaagcat
    13261 cgcttcggtg acgcgatagt ccgcgatgac cccgtctttc atagggcgat acgcaatgat
    13321 ggagtcaggc gtgcggccaa tcatcttctt cgcttcaaca cctatggcga gcactttgtt
    13381 gttttccgta gagaccgcga ctaccgacgg ctcgttcagt atcactccct ttccaggaag
    13441 gaacacgagc acattggtcg tgcctaggtc gatgcctagt ttcggcgaga aaaatgccat
    13501 ttcccccaca tcataccgtg caaggggccg aacctcaaaa actgtgtaaa accgagagcg
    13561 atgttagaat gcgcacatgc caaaagatga cctcagtttc atcgttaacg gagggaaaac
    13621 cttctccggg tccatactca ccaaccgctc caagaatggc gcggtgagcc tgttggcagc
    13681 ctccctcctt aataaaggaa cgaccactct ccataacgtg ccgaagattg aggaagtgaa
    13741 ccgtctcctt gaggttctct cttctattgg agttgaggcg gtctgggacg at
//
LOCUS       DAORIN010000006        13663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_761_length_13663_cov_5.530497, whole genome shotgun sequence.
ACCESSION   DAORIN010000006 DAORIN010000000
VERSION     DAORIN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_761_length_13663_cov_5.530497"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4524)
                     /locus_tag="PK109_00520"
     CDS             complement(<1..4524)
                     /locus_tag="PK109_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRH24063.1"
                     /translation="MDSLLDKNLITTREASELSGYHSDYLGRLCREGKISGSRVGRSW
                     VIDRSSILRFVEEQELQKKKNAEELAHSREEEYKAVQAAKTPISAPVVVPTTPAVVRA
                     VVPPAPAPVSIPSPYFSPKELQEKMVATLVAFLVITVGLYGSEAKFVERGARVAVETL
                     ENTSDIGTQAIASALKQGAVQLAAFRIGQEKFGMAFVSRVESGLVLYKGTITGAGEQL
                     ATDEVIAISSGAIDSLASQGSNGIVDTFIGSAVALGNTVVGASDAILNAHNNLATAWA
                     EGSAAVPPRLITAFYGAGTEAIALVSNVPSAIALGYENATYAWVASTQDLAMRITRAE
                     LSVGTVALERTNDVVIALTTPEVPSDGARGALAELFETRLTTPSTPTRSLAAASAATD
                     FFAPARSLALFTYETINGFFSRTGAVLAGMFAPNIAYAPEVSTIPTPLTVTSVTNIAT
                     SSSYITYITNPVSYIYSGTGGASIAYVQKAMDNLRRDLADRDDNNNSGGGLADITGSS
                     IEELSDVATMTKSFGDVFLWNGTEWTNVATSSLGIGAGTGAPGGASGQVQYNNAGVFA
                     GDAGFTYDVGAGRLTATYASTTGISTAYASSTLLRAGTAIIDSLSATLATLTGLIATD
                     ATTTNATSTNLAVMNSFALGADRFTDLTGPGLTASSGTLAVDDVTPAMLQAADFGAFT
                     CNGTSCALDAASISDTHINFGSVTLADFTNDAGFITSAITSVGPTGQMQTGSAITLAT
                     TSTAFNGLTASTTITASGNIITFANTLSGRLGVAGGGTGTTTAPTYGQLLVGDGSGGY
                     ALLATSSLGISGGTPGGANGQVQFNNAGAFGGSAAFTFDSTAGRLSASYASTTGFSSV
                     YASSTNLFAGNLTLGALSGILKASGGVVSVATAGVDYLTSAITSIGPAGALQTGAAIT
                     LATSTAGTDFTITAAGNTLTFNLPSASATNRGVLSSADWSVFNNKISSTSLSASAPLS
                     YNPSTGSFTITQATTGTNGYVSAADFTTFNNKISSTSLSAGAGISYVPATGVITNTGV
                     LTTSGDWTGTFDGQQGTYYLDRTNHTGTQLASTISDFASVARSLFSSSATGLTYTAGT
                     GAFSLTSGYVIPLVASTTQWDTAFSWGNHATAGYENALSFIYPLVRTVDAISLAFGTT
                     TANTWGAQQTFTGIFATNASSTNATTTNLTVNGAFRGAGLTSCSASGDKLLWNSTTGQ
                     FTCGADAGAGGGITAIGAQYSTFQTGSSQTFATSSDTNLGLIITSAGDTHTFMPTWIG
                     TLASGRLNSNVVQGVTNDTNVTGSISAQNLTLGWTGLLSVARGGTGWGNLQANTVLLG
                     NGTGAISTTSAGSNGQVLALVGGVPTWVATSSINNGVASIQQTGGGVAQTGAITFATS
                     SATNNGVTIGLNITNTGGAFTFTPSRSGTLTVAGGGTGVGTFTSGQLLYGNGTNALSS
                     VATTTFTPSGEFTTTGTIGALIGGSNSTISLATNGVALTKLAQIAANTILGNATGATG
                     NVTAIATSTLFGTG"
     gene            complement(5138..5935)
                     /locus_tag="PK109_00525"
     CDS             complement(5138..5935)
                     /locus_tag="PK109_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24064.1"
                     /translation="MDELTIEGKLYISSKRAAKLSGYAKDYIGQLCREGRVDSKLVGR
                     SWYVYEPSIREHRFNDERPRGKKNQIEEKIDTQSEEIQKSPIDTSWERPSYTSEVAET
                     LPELVTAVKASPEPETLGEMQSAWKEWFAERGAKAPQTSEREEEAAVEELPSITPAYS
                     APKVETVPVRVIVSDIKPIPQRQEERAESPTYSPQQASVRSQIKPKTRTKARIGGLVI
                     KSSFIAFIVILVSITVVATGLIEELHLGGLSDSPFLQYFQGSNVVEK"
     gene            complement(6461..7858)
                     /gene="gatB"
                     /locus_tag="PK109_00530"
     CDS             complement(6461..7858)
                     /gene="gatB"
                     /locus_tag="PK109_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRH24065.1"
                     /translation="MSDSSPYRATIGLEIHAELKTRTKMFCDSRNDPEEEKPNANICP
                     VCMAHPGTLPVINKEAVRHVLRVGTALGSTLADYTEFDRKSYFYPDIPKGYQISQYEH
                     PLVSGGILNGVAITRVHLEEDTARSSHAGGASLVDFNRAGVPLMELVTEPVIHDAATA
                     GAFARELQLLLRALGASDANLEKGEMRIEANVSVSKTDTPSVDYVEVKNINSFRSVER
                     AIAHEIERQIAVLENGERVVKATRGWDEGKQETFHQRFKEGSADYRYFPEPDLPKLVL
                     SEIPEFNAEALRASMPELPWEKRARLIARGVTEEATEILVGDPLYSDVYEREIQNITD
                     EKAHTAAVNILLGEVRGKNPTTEQLAHFNGTIFRTVMEHLVSGATSSTAAKSSIIGVL
                     INGGEPEIKVQMNDTDAIQKAVQEVIAANAGVAEEYRGGKVSVLQFLVGQAMKATKGA
                     GNPALIRELLEKELG"
     gene            complement(7874..8854)
                     /gene="obgE"
                     /locus_tag="PK109_00535"
     CDS             complement(7874..8854)
                     /gene="obgE"
                     /locus_tag="PK109_00535"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRH24066.1"
                     /translation="MALVDEVRIHAQAGHGGPGVTRWLHIRGQDKGGPAGGDGGRGGD
                     IILEGVRDLSALATFRFTKKFRAENGKPGGNDNKHGKDAEPIVLKVPVGTVAKIVTTG
                     QIYEIISDGQREVVFKGGPGGYGNAHFKSSTNQNPLEVTKGKPGGVGDIEITLKLIAD
                     AGLIGFPNAGKSSLLNALTRAQSKVGAYPFTTLDPHLGDLYGFLLADIPGLIEGASSG
                     RGLGSKFLRHVERTGLLIHLVSAEQEDVAASYRAIRKELEAFGRGLPEKPELIVLSKS
                     DMLSPEEQGKALAALAEASNGGEVTSVSIIDDDALKAFSDRLTSLLKSKA"
     gene            complement(8914..11427)
                     /gene="secA"
                     /locus_tag="PK109_00540"
     CDS             complement(8914..11427)
                     /gene="secA"
                     /locus_tag="PK109_00540"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRH24067.1"
                     /translation="MAGFLGRMFAKNESAAFVKAAGPIIAYAAQVEPELLELSQEALA
                     ARLAAVRERTGGVPTTDKDIAEVFALVREASRRTLKERHYDVQLMGGLALAKGNIAEM
                     RTGEGKTLVATLAATLRALAGKGVHLVTVNDYLARRDAAWMGQVYGYLGLTVGVVTSA
                     GNYVYDPSHVAADEDAKRDEEGSFRVFYDYLRPSTKKEAYAADITYATNNELGFDYLR
                     DNTAYEPSQINQRGHHYAIVDEIDSILIDEARTPLIISGPAADAEGLYERFAGIVRSF
                     EEGTDYTVDEKQRAVQITEGGITKAEAALGIENLYTEGGMKYAHQLETAVRAKALFRK
                     DKEYVIKDGEVVIVDEFTGRLQAGRRWSEGLHQAVEAKEGVPVKKETRTYASVTFQNY
                     FRMYEGIAGMTGTALSSEEEFYTVYKLEVVAVPTNRPVQRIDHDDLIFQTNKGKYKAL
                     AKAVKELHEKGQPVLVGTVSIEDNEVVSKALSDAGIPHEVLNAKNHEREGEIIAGAGA
                     KAKVTVATNLAGRGVDIKLGGALAGEEVREEVRRLGGLAVIGTERHEARRIDDQLRGR
                     SGRQGDPGATQFYVSLEDKLMRVFASDMVKNVMGTFKIPEDEPIQSGMITKSLETAQK
                     RIEGFNFDSRKQVLAYDDVLNTQRLSIYARRRTALLGSAEDVEATFQTIVAGDEAAQK
                     ALEEKRVEFGTAFPAVLRRLLLQVVDSFWLEHLDTMEYLRRSVSLRAYGQRDPLIEYR
                     REGLIRFNAMEASIDAALREALPRIHPANDAKIKQEEAKMRNAVLAASTAGTAQSPIE
                     KGTTYGRNDLVTIKNGDKTQTMKYKKAEPFIAEGWEIVL"
     gene            11526..11765
                     /locus_tag="PK109_00545"
     CDS             11526..11765
                     /locus_tag="PK109_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRH24068.1"
                     /translation="MAIKNIIGLKELRENTETYIERVGRGESITVVRRSTPLFRLTPV
                     DTEEVGWEPVADFTTIDEKGVSARDILASLRKMHG"
     gene            11758..12000
                     /locus_tag="PK109_00550"
     CDS             11758..12000
                     /locus_tag="PK109_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRH24069.1"
                     /translation="MDKIAKLLKKLSPKERERLEEVLGLLISGTTSSLDIKKLKGADE
                     VFRVRLGESRIIFQKRGGEIRILEVSRRDESTYKNY"
     gene            complement(12319..13335)
                     /gene="murB"
                     /locus_tag="PK109_00555"
     CDS             complement(12319..13335)
                     /gene="murB"
                     /locus_tag="PK109_00555"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRH24070.1"
                     /translation="MILTEHVPLATLTTLKVGGVATLLAQVTSVEEVAEAVALARERR
                     LPFYVLGEGSNVLPNDDGYDGVIIRVEIPGLSFTEEGSDTLLIAGAGVSWDAAVREAA
                     ARNLWGIENLAGIPGTMGAAPVQNIGAYGADLSQVLRSVDAYDAEKGEVVHIAAEDCA
                     LGYRDSRFKHEPNLIITSVTLALSATPAPRIEYGDLLAARERGDDLSTPAAIGDTVRA
                     IRSKKFPDLNEFGTAGSFFKNPVLTPDAYAALSEQHGEVPRFPNPNGVKVPLAFILDK
                     VLSLRGFTLGHASLFGAQPLVLVLEKGGKSVDIETLAQEVEKRVHEATGIKIEREVRT
                     MPTK"
     gene            complement(13332..>13663)
                     /locus_tag="PK109_00560"
     CDS             complement(13332..>13663)
                     /locus_tag="PK109_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24071.1"
                     /translation="KYAAVGKIITGDVAGGKALLTSAFGSPIVDDDLLRFAYYDAKLY
                     GDLIEVAKLHVTNTPNDPMARFLLAQAYAVAGRTAEARAEIQTTIVAYPAAAATGADL
                     LRQLGSQ"
BASE COUNT         3144 a         3483 c         3557 g         3479 t
ORIGIN      
        1 gccggtgccg aagagtgttg aggtggcaat ggccgtcaca ttccccgtcg cacccgtcgc
       61 attgccaaga atggtattcg ccgcaatctg cgcgagcttg gtgagagcaa caccgttggt
      121 ggcaagactg atggttgagt tcgagccgcc gatcaaggca ccgatggtgc cggtggtggt
      181 gaactctcct gacggggtga aggtggtggt cgcaaccgat gagagcgcgt tggtgccgtt
      241 gccatagagg agttggccgg aggtgaaggt gcccacaccg gtgccgccgc ctgcgaccgt
      301 gagggtgccg gaacgcgacg gggtgaacgt gaaggcgcct ccggtgttcg tgatgttgag
      361 gccgatggtg acgccgttgt tggtagcgga ggaggtggcg aaggtgatag cgcccgtctg
      421 agctaccccg ccaccggttt gctgaatgga agcgacgccg ttgttgatgg atgaggtggc
      481 gacccacgtt ggtaccccac cgaccaaggc gagcacttgg ccgtttgagc ctgcagaggt
      541 ggttgagatt gcacccgtgc cgtttccaag gagaacggtg ttcgcttgaa gattgcccca
      601 gcccgtgccg ccacgcgcaa ccgagagcag accggtccat ccgagcgtga ggttctgtgc
      661 tgagatggag ccggtgacat tcgtatcgtt cgtgacaccc tgcacgacgt tgctgttcaa
      721 gcggccggaa gccaaggttc cgatccacgt cggcataaac gtgtgcgtat caccggctga
      781 cgtgatgatg aggccaaggt tcgtatcaga ggatgtcgca aacgtctgcg aggagccggt
      841 ctggaatgtg gaatactgcg cgccgatagc ggtgatgccg ccgcccgctc ctgcgtccgc
      901 cccacaggta aattgtcctg tcgtcgaatt ccacagaagt ttgtcgcctg aagccgagca
      961 ggaggtgagg cctgcgccgc ggaatgcgcc gtttacggtg aggttcgtcg tcgttgcatt
     1021 cgtcgaagaa gcgttcgtcg caaaaatgcc ggtaaacgtc tgctgagcgc cccatgtgtt
     1081 agcggtcgtc gtaccgaatg cgagcgagat cgcatcaacc gtgcggacaa gcgggtatat
     1141 aaaggagagc gcgttttcgt atcctgctgt ggcgtgatta ccccaagaga aggcggtgtc
     1201 ccactgcgtc gttgaagcaa cgagcggaat gacgtagccg cttgtaagcg agaaggctcc
     1261 ggtaccggcc gtataggtga gtccggtcgc gctcgatgag aagaggctgc gcgcaacgct
     1321 ggcaaagtcg ctgatggtgg acgcaagctg ggtgccggtg tggttcgtgc gatccaagta
     1381 atacgtaccc tgttggccgt cgaaagttcc ggtccaatcg ccagaagtcg tgagtacgcc
     1441 agtgttggta atgacgccgg tcgccggcac atacgagata cctgctccag cggaaagcga
     1501 agtggaagaa atcttattat tgaaggtagt gaagtccgct gcggagacgt agccattagt
     1561 gccagtagtt gcctgcgtga tggtaaacga accggtggat gggttatacg agagcggtgc
     1621 agacgccgaa agtgacgtag aagagatttt gttgttgaag acgctccagt cagctgaaga
     1681 gaggacgccg cggttagttg cggacgcaga aggaagattg aaggtaagcg tgttgcccgc
     1741 tgcggtgata gtgaagtcag ttcctgccgt tgacgtagcg agggttatcg ccgcaccggt
     1801 ctgcagcgca cctgcaggcc cgatggaagt tattgcagag gtgaggtagt cgacgccggc
     1861 agtggcgacc gatacaactc cgccgcttgc cttcaagatg ccggagagcg cgccgagcgt
     1921 gaggtttccg gcaaaaagat tggtggagga ggcgtacaca gagctgaatc cagtcgttga
     1981 agcgtatgaa gcgctcaagc gtcctgcggt tgaatcaaac gtaaacgcgg cgctgccgcc
     2041 aaacgcgcca gcgttattga actgcacctg tccgttagct ccgcctggtg taccgccgct
     2101 gataccaagg gaagaggtag cgagaagcgc gtagccgccg ctgccatcgc caaccagaag
     2161 ctgaccgtaa gtcggagcag tcgtcgttcc tgtaccgcca ccggcaacgc cgagacggcc
     2221 tgagagcgta ttagcgaagg tgatgatgtt gccagacgca gtaatggtcg tcgaggcagt
     2281 gaggccgtta aacgctgttg aggtcgttgc aagcgttatt gcagacccgg tctgcatctg
     2341 accagtagga ccaactgagg taatcgctga cgtgatgaag ccggcatcgt tcgtgaagtc
     2401 agcgagcgta acggagccga agttaatgtg cgtatcggaa atagaagctg catcgagtgc
     2461 gcagctcgtt ccgttacacg taaaggcacc gaagtcagct gcctggagca tcgctggagt
     2521 tacatcatca accgcgagag tgccggaaga agcggtgaga ccaggacccg taagatctgt
     2581 aaatcgatcg gctccaagcg cgaacgagtt catcaccgca agattggtcg atgttgcgtt
     2641 agtcgtcgtc gcgtcagtgg cgataaggcc ggtcaacgtc gcaagcgttg ctgagagtga
     2701 atcgataatt gccgtgccgg cgcgaagtaa cgtggatgag gcgtacgcgg tagagatgcc
     2761 cgtagtagac gcgtacgttg cggtaagtct tccggcacca acgtcataag taaatcctgc
     2821 gtcacccgca aagacgccag cattgttgta ctgcacttgc ccggaagcac caccaggagc
     2881 acccgtacca gcaccaatac caagcgagct cgtcgctacg ttcgtccatt cagttccgtt
     2941 ccaaagaaat acatccccga aggacttcgt catcgtagca acatcagaga gttcttcgat
     3001 ggaagaaccg gtgatgtcag cgagaccgcc tcccgaattg ttgttatcat cgcggtccgc
     3061 aaggtcgcga cgaaggttgt ccatcgcttt ctgtacgtac gcgatagagg caccgcccgt
     3121 accgctatat atgtagctca ccggattagt gatgtacgta atgtatgagg aggacgtagc
     3181 gatgttcgta accgacgtaa cggtaagtgg cgtaggtatc gttgatactt ctggagcgta
     3241 tgcaatgttt ggcgcgaaca tgccagcaag tacagcgccg gtacgactaa agaatccgtt
     3301 gatggtctca taagtaaaga gtgctaggga acgagcagga gcaaagaaat cggttgccgc
     3361 tgacgcagca gcgagcgagc gagtaggagt agacggcgtc gtgaggcgtg tttcaaaaag
     3421 ctcagcgagt gcaccgcgtg ctccgtcgct tggtacttca ggagtagtga gtgcaataac
     3481 aacatcgttg gtacgctcta atgcaactgt gcctacagaa agttcagcac gcgttatccg
     3541 catcgcaaga tcttgcgttg aagcaaccca tgcataggtt gcgttctcgt atccgagtgc
     3601 aatagcagac ggaacgttac taacgagcgc aattgcttct gtgccggcgc catagaacgc
     3661 agtgatgagg cgaggcggta ctgcagcact tccttctgcc catgccgtag caagattgtt
     3721 gtgagcgttc agtatggcgt ctgaagctcc gaccaccgta ttaccgagag cgacagcact
     3781 accaatgaag gtatcgacga ttccatttga accctgactt gcaagactat cgatagctcc
     3841 cgagcttatg gcgatgactt cgtccgtagc aagctgttcg cccgctccag tgatcgtacc
     3901 tttatatagt acgagtccgg actctaccct tgatacgaac gccattccga atttctcctg
     3961 gccaatgcga aaagccgcaa gctgcactgc accttgtttg agtgctgatg caatagcttg
     4021 agttccgata tcgctagtat tttcaagggt ttctacggca acgcgtgctc cgcgctcaac
     4081 aaactttgct tctgatccgt agaggccgac agtgattaca aggaacgcaa cgagcgttgc
     4141 caccatcttt tcctgcagct cctttggaga aaagtatgga cttggaattg agactggagc
     4201 gggagcggga ggcaccaccg cacgcactac tgcaggtgta gtaggaacca ctacaggagc
     4261 tgatataggc gtcttagcag cctgcacggc cttgtattct tcttcgcgtg aatgcgcgag
     4321 ctcttcagcg tttttctttt tttgcagttc ctgctcttca acaaatctca aaattgaaga
     4381 gcgatcaata acccatgatc gtccaacacg acttccagaa atttttcctt cgcgacaaag
     4441 gcgtccgaga tagtccgagt gatacccgga aagctccgat gcttcgcgag tcgtgattaa
     4501 gtttttatcg aggagagagt ccatgtcgga tatccgaagt aactatattt tacgtcgtgt
     4561 aattcattct tccctaatat tttttgggga taagatgaaa ttaaattcat aatctaaaag
     4621 tttagaaata ttttttgcaa gaaaacttaa aaaataattt caaatacggt taccaagagt
     4681 gtgaattcaa ggaaaattgc tgaaaaatct cactgattgt taaaagaacg atggtgatta
     4741 agtaatttcg ataaaatcat tcaaaaagtt ccgaaatgac taataaataa gccattattt
     4801 ttgtaattaa cgctgctaac gatgtgattc ttttttgacg actcaagcta agtaagagag
     4861 cagcggcata gtgaaaagga gttagattaa aaccaacaat ctaagtaaat attttctaaa
     4921 taacgagctt ggcgtacgat caatgatgat ctgtgtctct ttttctttca aaaatatata
     4981 aaaagtacgt aattatcgat gttttcttag aaaattcctt gtatacgcac tattgatgaa
     5041 atacaaaaat aaaaaacgac actcggtgcc gtttttattc tctactgcat ctattattta
     5101 ttcttgttta tacgagacaa gatatataag taataggcta tttttctact acattggaac
     5161 cctggaaata ctgtaagaat ggagaatccg atagcccacc taaatgtaat tcttcaatca
     5221 atcctgttgc tacaacagtt atgcttacca gtataactat gaatgcaatg aagctagact
     5281 tgataacaag tccgcctata cgggctttgg tcctggtttt aggctttatt tggctacgta
     5341 ctgacgcctg ttgaggagaa tatgtaggcg actctgcgcg ttcttcctga cgctgcggta
     5401 ttggcttaat atcactcacg atcacacgta ccggtactgt ttctaccttg ggagcgctgt
     5461 atgcgggcgt tatggaggga agttcttcga ctgcagcctc ttcttctctt tcagacgtct
     5521 gtggagcttt tgctccgcgc tcagcgaacc attctttcca tgctgactgc atttctccaa
     5581 gcgtctctgg ttctggagac gcttttactg cagtcacgag ctctggcaac gtctcagcaa
     5641 cctctgaagt gtaggaagga cgttcccatg aggtgtcgat aggagatttc tgtatttctt
     5701 cgctttgagt gtctattttt tcttctatct ggttcttttt acccctggga cgctcgtcat
     5761 tgaagcgatg ttcccggata gagggctcat atacgtacca actacggcct acaagctttg
     5821 aatccacccg accctcacgg caaagttggc ctatgtagtc ttttgcgtat ccacttagct
     5881 ttgccgcccg cttagacgag atatatagct tcccctcaat agtaagttca tccatacctt
     5941 gtagtatata ggtatcttgt ttgaacgaga aatgcaggaa aataaggatg agggatagag
     6001 gccttgctct ccaggaccca ctcttcaggg gtttcttact gtatcgacga ctcttttaca
     6061 ggagaggctt cttcttcaat tactggcggt acttgttctt cagcaggagg ggtaatcatt
     6121 tcttgtaagt ggatctatag agctcccctg ctttaatgcc aggatatggt ggagtcaggg
     6181 tggtttaggc gtggctatcg gtttcttaag catcacctct gcattcgcat cccatgtagc
     6241 gagtagcttt tggttcttaa caccgatagc tactccacat tcaacgcctg acttgagttt
     6301 agatcttaaa attactcgaa agtagaagcc ttatttatga aaagatatat tattaagttt
     6361 atataatata catttctcgt ctgttctatc cgagatatat agtggcgtat atacaaagta
     6421 aaaagacgct catgcgtctt tttacatctc tctcctattg ttatccaagc tccttttcga
     6481 gcaattctcg tattagtgcg gggttaccgg cacccttagt tgccttcata gcctgtccta
     6541 cgaggaactg gaggactgac acctttcctc ccctatactc ttctgccacc cctgcattgg
     6601 cggctataac ctcctggact gccttctgga tggcgtccgt atcgttcatc tggactttga
     6661 tctctggttc gccaccattt atcagtacgc caatgatgct tgatttcgcg gccgtagatg
     6721 aggtagctcc cgatacaaga tgttccatta ccgtgcggaa gatggtgcca ttgaagtgcg
     6781 cgagctgttc tgtagttgga ttcttgccac gtacttctcc aagaagaatg tttactgctg
     6841 cggtgtgtgc tttctcatca gtaatgtttt gtatctcacg ttcatatacg tcactataga
     6901 gcggatcacc gacaagtatt tcggttgcct cttctgtaac accgcgcgca attaatcgtg
     6961 cacgcttctc ccagggaagt tctggcatag atgctcttag tgcctcagca ttaaattctg
     7021 ggatttcgga gagtaccagc tttggaagat caggttctgg gaaataccga taatcagcac
     7081 tcccttcctt gaagcgctgg tgaaaggttt cctgcttacc ttcatcccat ccgcgcgtgg
     7141 cctttacgac ccgctcccca ttttcaagga ctgctatctg acgctctatt tcatgtgcga
     7201 ttgctcgctc aacggaacgg aaagagttta tgttttttac ttcgacgtaa tctaccgatg
     7261 gtgtgtctgt cttcgagaca gaaacgtttg cctcaatgcg catctctcct ttctcaaggt
     7321 ttgcgtccga tgcaccgagc gcacgcagca ggagttgaag ttcgcgtgcg aacgcgcctg
     7381 cagtcgcagc gtcatgaatg actggttcag taactagttc catcaacgga acacctgcac
     7441 gattgaaatc gacgagacta gcgccgcctg catgtgaaga acgtgctgtg tcttcttcga
     7501 gatgtacacg agtgatagca acgccattca atatcccgcc tgaaacaagc ggatgttcgt
     7561 actgactgat ttggtatccc tttggaatgt ctgggtagaa ataacttttc cgatcaaact
     7621 ccgtatagtc tgcaagtgtt gatccgagtg cggtacccac gcgtagtacg tgacgtactg
     7681 cttctttatt gataaccggc agagtcccag gatgcgccat acatacggga cagatgtttg
     7741 cgtttggctt ttcctcctcg gggtcgttac gggagtcgca gaacatcttc gtgcgggttt
     7801 taagctccgc atggatttct agtcctatgg tggctcggta cggggaggaa tcactcattc
     7861 cccctatcct gacttaggcc tttgatttga gcaagctcgt gagacggtcc gaaaaggcct
     7921 tgagggcgtc gtcatcgatt atggaaactg aggtaacttc ccctccgttt gaggcttctg
     7981 cgagtgcggc gagcgccttc ccctgctctt caggagagag catgtcagat tttgagagca
     8041 cgataagctc cggcttctcc ggaagaccac gaccaaaggc ttcaagctcc ttccgtatgg
     8101 ccctatacga agcagcaaca tcttcttgtt cagccgagac aagatggata agtagaccgg
     8161 tacgttctac gtgtcgaagg aatttagacc ctaaccctcg tccagacgag gcaccctcaa
     8221 taaggccagg aatatcagca aggaggaacc catagaggtc accaagatga ggatcaagcg
     8281 tagtaaatgg gtaagcaccc accttagact gggcccgagt gagtgcgttc aaaagagatg
     8341 acttccctgc attcgggaac cctatgagac cggcgtccgc gatgagttta agggtgattt
     8401 cgatatcccc aactcctcct ggtttcccct tcgttacctc aagcgggttc tggttggtgg
     8461 agcttttgaa gtgggcattg ccgtatcctc cagggcctcc tttgaacacc acttcacgct
     8521 gaccgtcact aatgatttcg tagatctggc cggtcgtaac gattttagcg acggtaccta
     8581 ccggtacttt aaggacaata ggttcagcat cttttccgtg cttgttatcg ttcccgcctg
     8641 gcttaccgtt ctctgcgcga aactttttag tgaagcgaaa ggttgcaaga gcgctcaagt
     8701 cgcgtactcc ctcaagaata atgtcgccgc cgcgaccacc gtcgcctcct gcaggtcctc
     8761 ctttatcctg gccgcgaata tgcagccagc gcgtaacacc agggcctccg tgccctgcct
     8821 gcgcatgtat gcgaacctca tcaacgagtg ccatagtcct tcccttataa cacaaaaacc
     8881 ccgtttacgg ggttttgttt acgtaacgct aagttagaga acaatctccc agccttcagc
     8941 aataaatggt tctgctttct tatatttcat ggtctgggtc ttgtcgccat tctttatggt
     9001 tacaagatca tttctcccgt acgtcgttcc cttctcaatt gggctttgtg ccgttccggc
     9061 agtagaggcc gcaagtactg cgttacgcat cttggcttct tcctgcttta tctttgcatc
     9121 gttcgctgga tgtatacgtg gtagtgcctc acgcagtgct gcgtctattg atgcttccat
     9181 agcattgaag cgtatgaggc cttcacgacg gtattcaatc aatgggtcac gctgaccata
     9241 cgcacgaagt gatactgagc gacggaggta ctccatggta tcgaggtgct cgagccagaa
     9301 ggagtccacc acctggagca ggaggcggcg cagcaccgct gggaacgcag tgccgaactc
     9361 tacccgcttc tcctcaagcg ccttctgggc agcctcatct cctgcgacta tggtctggaa
     9421 cgtagcttct acatcttctg cagacccaag aagcgcggta cggcgacgag cgtatatgga
     9481 cagacgctgc gtgttaagca cgtcgtcgta ggccagtacc tgcttgcggg agtcgaagtt
     9541 gaacccctcg atgcgcttct gggcagtttc aagtgatttg gtgatcatgc cgctctgtat
     9601 cggctcatct tccggaatct taaaggtgcc catgacgttc ttgaccatgt cagaggcgaa
     9661 tacgcgcata agcttatcct caagagaaac ataaaactgg gtagcgcctg ggtcaccctg
     9721 gcgaccagaa cgtcctcgaa gctgatcatc gatacgtcgt gcttcatggc gctcggtacc
     9781 aataaccgca agaccgccga gcctacggac ttcttcacgc acctcttctc ctgcaagcgc
     9841 tccgccaagc ttgatgtcga cgccgcgccc tgcaaggttc gtcgctacgg tgacctttgc
     9901 tttggcgcct gcgcctgcga tgatttctcc ttcacgctcg tgattctttg cattcaacac
     9961 ttcgtgcgga ataccggcgt ctgaaagcgc cttacttacg acttcattgt cttcaatgga
    10021 aacggtaccc accaagaccg gctgcccttt ctcgtggagc tctttcactg ctttcgcgag
    10081 tgctttgtat ttccctttat tcgtctggaa tataaggtca tcgtggtcga tacgctgtac
    10141 cggacgattc gttggtaccg cgactacttc aagcttatat acggtataga actcttcttc
    10201 tgaagaaagt gcggtaccgg tcataccggc gataccttcg tacatacgga aatagttctg
    10261 gaaggtaacg gaggcatagg tgcgcgtctc ctttttcact ggtactcctt cctttgcctc
    10321 aaccgcttga tggaggcctt ctgaccaacg gcggcctgcc tggagacgcc cggtgaactc
    10381 atccacaata acgacttcgc cgtcctttat gacgtattcc ttatctttac gaaatagcgc
    10441 tttggcacgt accgcagtct cgagctgatg cgcatacttc atcccacctt cggtgtagag
    10501 gttctcaata ccgagcgctg cttctgcctt cgtgatgcct ccttcggtta tctgtaccgc
    10561 acgttgtttc tcatcaaccg tgtagtcagt tccctcttca aacgagcgaa cgataccagc
    10621 aaatcgttca tagagccctt cagcgtctgc tgcagggccc gagatgatga gcggggtgcg
    10681 tgcttcatcg ataagaatgg agtcaatttc gtcgacaata gcgtagtgat ggccgcgctg
    10741 gtttatctgt gatggttcgt acgccgtgtt atcgcgaaga taatcgaacc cgagttcgtt
    10801 gttcgtcgca tacgttatgt cagcagcata cgcttccttc ttagtcgatg ggcgcaggta
    10861 gtcatagaac acgcggaagg agccttcctc gtcgcgcttt gcgtcttcgt ctgcagcaac
    10921 gtgcgaagga tcgtacacgt agttcccggc tgaagtcacc acgcccaccg tgagtccaag
    10981 gtatccgtac acctgcccca tccatgccgc atcgcgccgt gcgagataat cgttcaccgt
    11041 caccaagtga actcctttcc cggcgagcgc ccgcaaggtt gcggcaagcg ttgcgacgag
    11101 tgtctttccc tctccggtac gcatctcagc aatgttgcct ttggcgagtg cgagtccgcc
    11161 cataagctgc acgtcatagt gacgctcttt tagggtgcga cgtgacgctt cgcgcacgag
    11221 tgcaaatact tcagcgatat ccttgtccgt ggtaggtacc ccaccggtgc gctcacgtac
    11281 tgctgcgagc cgtgcggcga gcgcttcctg ggaaagctcc aataattcag gctccacctg
    11341 ggctgcgtac gcaataatag gccccgcagc cttcacaaaa gctgcggatt cgttctttgc
    11401 gaacatgcgc ccgaggaagc ccgccataat ggcgcactat aacattttta gcctttttct
    11461 gccttctgca cggagatcta cattgagtac ttgactaaaa gattatctta gtataatctt
    11521 ttagtatggc tataaaaaac atcatcggac tcaaagagct tcgagagaac accgagacct
    11581 acattgagcg tgtgggtcgt ggcgaatcta taaccgtcgt ccgccgctcc actcccctct
    11641 tccgcctcac cccagtagat acagaggaag tcggctggga accggtcgct gactttacta
    11701 ccattgacga gaagggcgtt tctgcacgag atattcttgc ttcccttcgg aaaatgcatg
    11761 gataagattg ctaagctcct taagaaactt tccccaaaag aacgcgaacg ccttgaagaa
    11821 gtgcttggtt tgcttatatc tggcactacc tcttccctag atataaagaa gctaaaagga
    11881 gcagatgagg tgttccgagt acgtctcgga gagtcacgca tcatttttca aaaaagaggc
    11941 ggggaaatcc gcattcttga agtgagccga agagacgaga gtacctacaa gaactactaa
    12001 tcctatataa gaaaaaaccc cgcggaggcg gggctttttc tcgaaagcgc tatgcgcgct
    12061 ttgcagtctt gcggcgcttg gtggtcttct ttgcgacctt ctttttggtc gtcttcttcg
    12121 cctttttctt tgcagccatg gtgcggaaat tagaatgtct ggtaatcgac ccactgtcgc
    12181 gcgagcaaat tcgtgagaat cttttcgcgt gaagtgttct actacgtatt attgcgcgca
    12241 aaaaataaaa tagtgaaaga aaagtgtgag ctgtggataa ctttgtaaaa aacttttaca
    12301 aaatattttc tttaaaattt attttgttgg catcgtgcgc acttcgcgtt ctatttttat
    12361 tcccgttgcc tcatgcacgc gcttctctac ttcttgtgcg agtgtttcta tgtcgacact
    12421 cttccctcct ttctcaagta cgagcaccag tggctgtgca ccaaagagtg atgcatggcc
    12481 gagtgtaaat ccacgtaggc taagtacctt atcgagaatg aatgcgagcg gtactttcac
    12541 cccgttcggg ttcggaaagc gtggtacctc accatgctgc tccgagagtg ccgcatatgc
    12601 gtcgggcgtc agcaccggat tcttaaagaa ggaaccagca gtaccaaatt cgttcaagtc
    12661 tggaaatttc ttcgagcgta tggcacggac ggtatcccct atggctgctg gggtagagag
    12721 atcgtcgccc cgctcacgtg ccgcaagcag atctccgtac tcaatacgcg gtgcaggcgt
    12781 tgccgaaagc gcgagagtta ccgacgtgat gatgagattc ggttcatgtt tgaaacgact
    12841 gtcgcgatat ccaagcgcgc agtcttctgc agctatatgc accacttccc ctttctcagc
    12901 atcataggca tctactgaac gcagtacctg agaaagatct gcgccatacg cgccgatgtt
    12961 ttgtacggga gccgcaccca tcgttcctgg aatgcctgca agattttcta taccccagag
    13021 attgcgcgct gctgcttcgc gtactgcggc atcccaagaa accccagcac ccgcaatgag
    13081 aagagtgtca gatccttctt cagtaaatga aagtcccggt atttcaacgc gaatgataac
    13141 gccgtcatac ccgtcatcgt ttggaagtac gttactccct tcaccaagta catagaacgg
    13201 aagtctccgt tcgcgcgcaa gcgccaccgc ttcagctacc tcttcaaccg acgttacctg
    13261 cgcgagaagc gtcgctactc cgcccacttt gagcgtggta agcgttgcga gcgggacgtg
    13321 ttcggtaaga atcattgact accgagttgg cgcaaaagat cagcgccggt agctgccgct
    13381 gctggatacg cgacaatggt ggtctgtatt tctgcccgtg cttcagcggt gcgtccggca
    13441 accgcatatg cctgtgcaag caggaagcgt gccatagggt cgtttggcgt attggttaca
    13501 tgaagctttg ccacctcgat aaggtctcca tagagcttgg cgtcatagta cgcgaagcgg
    13561 agaagatcat cgtcgacgat gggcgacccg aatgcgctcg taaggagcgc ttttcctccg
    13621 gcaacgtccc cggtaattat tttcccgacg gcagcatatt tag
//
LOCUS       DAORIN010000007        13617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_763_length_13617_cov_5.627120, whole genome shotgun sequence.
ACCESSION   DAORIN010000007 DAORIN010000000
VERSION     DAORIN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13617
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_763_length_13617_cov_5.627120"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..128
                     /locus_tag="PK109_00565"
     tRNA            56..128
                     /locus_tag="PK109_00565"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89..91,aa:Phe,seq:gaa)
     gene            134..1225
                     /locus_tag="PK109_00570"
     CDS             134..1225
                     /locus_tag="PK109_00570"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HRH24072.1"
                     /translation="MAAFGTFNRVLAWSARNERPLGAVLFAFGFLTDFFTFGVLPIAV
                     VNYFFIGYLSLAAVAAIGAHAFARYHDGEPGVRKALAVLFPLGVQYAFGGLLSGFVVF
                     YTAHSVVLASWPFLIFLALVYIGNEYFRMYKHYLVFQTSLFFFTLYAYAIFGLPLVIG
                     RIGPWTFLGSTVVAILLFAAFLCLLRLGNKARFDASVRQIALACVGIVVLISVSYFTG
                     VIPPVPLVMKDAGVYHSLVKVPTGYRVQAEAPKEWWDIRPTVVHVLPGEALYAYSAVA
                     APISFSSTVVHRWEQYIDGKGWVTQSRISFPLSGGREGGYRGYSIKDTLTPGKWRISV
                     ETAGGQVIGRVRFDIENAGTAPALQEITL"
     gene            complement(1226..1645)
                     /locus_tag="PK109_00575"
     CDS             complement(1226..1645)
                     /locus_tag="PK109_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24073.1"
                     /translation="MKGLEGLEPSGEKLKLAIDEGSTSSIEPGAAEKLHSFLSEFTSE
                     RDLAAHARVRYNTEGAPASEAVRAEDVYLDKVRDAANRMNREDLGALMQHLRGAIKSN
                     LEARDFSDVSIESERRDMETSAYTTIESVWNKRARVH"
     gene            complement(1652..2134)
                     /locus_tag="PK109_00580"
     CDS             complement(1652..2134)
                     /locus_tag="PK109_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRH24074.1"
                     /translation="MKKRSLFDRLAGTRSEDDYDFDDDMMEAGGGKHLNVSSGMSAPH
                     PAHEERTEGHLPVDMYQTGSEIIIRTFVAGVRPDEMNVSISRDMVVVEGSREERSEAT
                     GPDYFNQELFWGTFARTIMLPQEIDVDGAQASSKDGLLTIILPKLDKTRQTKLKVRSS
                     "
     gene            complement(2139..2891)
                     /locus_tag="PK109_00585"
     CDS             complement(2139..2891)
                     /locus_tag="PK109_00585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRH24075.1"
                     /translation="MPSLETIGYALVGGLLPALAWLYFLLKEEDRCPQPRWAVVLAFV
                     CGMIAVPIVMPLQALACREWAISVVLACSGGETTLLGTKVLIAWSALEETAKYAVAAA
                     FVLWRRHAVRNSLDIVATMLTVALGFAALENALFLLAPFSQGYLLEAIGLGNLRFIGS
                     TLLHVVASSIIGFALAFSYKSSRPVRAIAASIGLILAIALHAVFNFFIIQGDGSYVIL
                     ALFTVWTGAVAIFAAFEILKYFLYRNLPKNTC"
     gene            complement(2891..5026)
                     /locus_tag="PK109_00590"
     CDS             complement(2891..5026)
                     /locus_tag="PK109_00590"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRH24076.1"
                     /translation="MDDKFLKPYQPGETESRIYETWEKSGYFNPDKLPAELLAPTEGK
                     AFTIVMPPPNANGNLHAGHALFVTLQDIVTRFKRMRGYRALWIPGADHAGFETQVVYE
                     KQLEKEGRSRFGMDPKALYEEILTFTLANKKNMVGQLRALGASCDWSREKFTLDPDLV
                     KKVQATFVKMFNDDLVYRGARTVNWCPKHQTSLSDVETDSVEETTPFYTFKYGPFDIG
                     TVRPETKFGDKYVVMHPDDKRYSKWKHGDTLEVEWINGPITATVIKDNAADMEFGTGV
                     MTITPWHSQIDFEIAKRHSLPMEQIIDWRGKLLPIATEFAGMNIKDARGKIVEKLASK
                     GLLVSTDDAYKNVVKKCYKCQAMIEPQVKDQWFVKMAPLANLAITAVNKGDVRFIPEY
                     EEKTFRHWMENALDWNISRQIVWGIPIPAWHKDGEWKASVESPGEGWTQDADTFDTWF
                     SSGQWPLLTTEYPDGKDFSQFYPTHLMETGRDILFFWVARMMVFGLYLTDQVPFPTVY
                     LHGLVNDANGKKMSKSKGNVINPLDLTDKYGTDALRMALVVGNTPGTDLALREDKVKG
                     YKHFANKVWNIARFVLENAGDADLTTPLHADDEALARELDEIAAKATTNLDEYRAYLA
                     ADAIYHYIWDRFAAEILEESKPIFGSHDTPSTFPPERIASRKALMMRNLQICLALLHP
                     FMPFITEEIWASTGTNTLLMASPWPILEG"
     gene            5147..5449
                     /locus_tag="PK109_00595"
     CDS             5147..5449
                     /locus_tag="PK109_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24077.1"
                     /translation="MDEKRKAEVTRALLRLRLWENGLTLSSSTNRKLGAFAKESGTSL
                     QELRELLIEFLPDFLGEALGADFVSIRVGYDDLDGDLKKVRHYVDAEHEFHRQASA"
     gene            complement(5590..6759)
                     /gene="tsaD"
                     /locus_tag="PK109_00600"
     CDS             complement(5590..6759)
                     /gene="tsaD"
                     /locus_tag="PK109_00600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRH24078.1"
                     /translation="MKVLAIETSCDETAVAVLECAGDASAATFTVLGNALLSQIDIHK
                     EYGGVFPTLAKRAHAENLTPLLGNALEEAGLLVTGTHTLSEEKKVRIQELLERETGLG
                     GAFIAFADATERPSIDAIAITHGPGLAPALWVGVNFAKALAAAWDIPLVPINHMEGHF
                     LAGLGKSEGNVITIENAQLPILGLLISGGHTELDAMTDWLTYTNLGATRDDAVGEAFD
                     KVARMMGLPYPGGPEISKLAERDRKENRENPFKLPRPMKDTDDFDFSFSGLKTSVLYQ
                     IRDKGDLTDIEKEQMARAFEDAVTDVLWTKTSRAIDKTGAKTVVIGGGVSANHHIQKE
                     FEAKIAEEYPNVDLRIPARTLSTDNAVMIGIAGYYRASRKEFSADFIADGNLSLA"
     gene            complement(6740..7603)
                     /locus_tag="PK109_00605"
     CDS             complement(6740..7603)
                     /locus_tag="PK109_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24079.1"
                     /translation="MERTVILYHDNCPDGFGGAYAAWKKFGDTAEYLPVQHQAETPAG
                     LKGAHLYLIDFCYPKDIMNVLAAEAASLTVLDHHEGVEDVVEAMPAYVYDAKRSGATI
                     AWSYFHPKTPVPVFLDIVMKADLYQMLTDDERAIISYAYAQPYSFSGWDTLTERVENP
                     ESRAEIIGRGRAYAEYFQLLATQIASRARRVRFEGHEVYLISAPRMFATELGTQLRKK
                     GGAFTLITRNDAHGSMRVSMRAGDNKTVNLAELAQKYGGNGHPGSAAFSLEWGAPIPW
                     EVIEHDESSSD"
     gene            complement(7620..8621)
                     /locus_tag="PK109_00610"
     CDS             complement(7620..8621)
                     /locus_tag="PK109_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HRH24080.1"
                     /translation="MNPIAKLFVLPVLVSILLLAGVFIWGGFHALALAVLLTILEVTL
                     SFDNAVVNARVLTKMNAKWQQRFLTWGILFAVVITRFVLPIFIVAVSVAMSPWAVAQL
                     AAFDPEHYGELLEGAHYAISSFGGAFLLMVSLKYFFDERKDIHWIRSVERVLARFGRI
                     EALELGLALTLLAVMAFLLPAQSATILFSGIIGIILFIAMQGVANSFTLETGKAVAAG
                     SAALFVYLNVLDAAFSLDGVIGAFAITSQLPVIVAGLGIGAYFVRTLTVFLVRQKTLD
                     TLIYLEHGAHWAILGLAAAMFASLFYQVPEVITGLVGLVFVGAAYWSSLQEQKKAEA"
     gene            complement(8627..8699)
                     /locus_tag="PK109_00615"
     tRNA            complement(8627..8699)
                     /locus_tag="PK109_00615"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8664..8666),aa:Val,seq:gac)
     gene            8746..9366
                     /locus_tag="PK109_00620"
     CDS             8746..9366
                     /locus_tag="PK109_00620"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRH24081.1"
                     /translation="MEIIRLTEGNLIEAAKKAAAVLKKGGIVAYPTDTVYGLGVSITD
                     PTAIERLRTLKGRERKKPVSLIVQDVHHIEMCGTLTPLAEKFAASHLPGALTLVIPAK
                     KDMPETLTMNGAVGVRVPNEPFCLALAKEYGKPYTTTSANKAGMGTPRNVQGLMEHFG
                     LSLDQIDLIIDGGDRAGGNPSTVVSCVGEIPYVLREGAVSRQELGL"
     gene            9409..9915
                     /locus_tag="PK109_00625"
     CDS             9409..9915
                     /locus_tag="PK109_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24082.1"
                     /translation="MKEMNEGWESSARNVQRIEQIISQPIVEEQIALHVRNIREILLT
                     SNSRNAESDSKIGKVLEDYARLIAMKFEQVKDIEPIASSDPNLAETLSSKTFPIAKKY
                     LEALMDGKFLAERLSDDMGTSKDWLNEEAESVLAMIAVNIMHDTLVVAYCLRLGIDPE
                     RLRTSSAT"
     gene            9928..10341
                     /locus_tag="PK109_00630"
     CDS             9928..10341
                     /locus_tag="PK109_00630"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRH24083.1"
                     /translation="MPEEALQFIATQRVGVLAVRMPDGSPHAATIHFAYVPTLGKYIF
                     RTSPESRKVESVKQGETTGSFVIGSSEAEMKTLQMDGVVKLEDSEEIQEAYLGGLTDK
                     SKESPDVYLTFTPTWWRYTDWTLPQGKTIFSSDAK"
     gene            complement(10379..11635)
                     /gene="ahcY"
                     /locus_tag="PK109_00635"
     CDS             complement(10379..11635)
                     /gene="ahcY"
                     /locus_tag="PK109_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRH24084.1"
                     /translation="MKHDIADESLAALGKKRLAWADNDMPVLAKVRERFAKEQPFKGK
                     TIGACLHVTAETGTLMRTLKAGGANVVLCASNPLSTQDDVAASLVVDEGIPVFAKKGE
                     DRDTFFSHLNAVIGENPHLTMDDGADLVSTIHKDYPQLIDSIIGGTEETTTGVIRLRA
                     MAKDGALKFPIVAVNDAKTKNLFDNRYGTGQSTLDGIVRATDILLAGKVFVAAGYGWC
                     GRGLALRARGMGAQVIVTEIDPVKAIEATMDGFRVMTMQEAAPLGDVFCTATGDIHVI
                     RMEHMKAMKDGAIICNTGHFDVEIDVAGLKAAASSVRENVRPFVDEYTVGKNRIFLLA
                     DGRLVNLAAAEGHPASVMDMSFATQALACEWMATQSEKLAPAVYDVTDEIENMVAGTK
                     LASMGITIDELTDEQKKYLADWEHGT"
     gene            complement(11639..12577)
                     /locus_tag="PK109_00640"
     CDS             complement(11639..12577)
                     /locus_tag="PK109_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRH24085.1"
                     /translation="MKPILVCGSIAYDRIMDFDGNFSEHFMPDKLHSISVSFFVSPPK
                     ENFGGTGGNIAYSLSLLETKADVVATAGNDFDRYGAWLADHQVGVATIARVKDVPTAS
                     GYIMTDKKDNQITAFSGSAAMQAYTNAIAYADYSAAIVSPTNKDDMVRIARESKAASL
                     PFFFDPGQQIPMLSAEELREAITDSAGMLVNDYELSLIGEKTGWNEAAIAEQVGFLIV
                     TLGAEGSRIITKNSEERVAAVAVTTVVDPTGAGDAFRGGFLAGYVRELPLPVCAKLGS
                     TVAAYAVECYGTQNHRFTLDELKARYEGAYNESFPI"
     gene            12610..13410
                     /locus_tag="PK109_00645"
     CDS             12610..13410
                     /locus_tag="PK109_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24086.1"
                     /translation="MKRYVVLDKKVGETPLRALTDWQNAHPNYAGMPASYAGRLDPMA
                     SGKLLILLGEECKKQKKYIGLDKEYVIEVLLDVGSDTGDALGLIDYPNRDTRSNDSFL
                     TKVLHAERGTHKRAYPAFSSKTVNGKPLFLHTLEGSIKDIAIPEHDEHIYRCSLLRAE
                     RIHASALKARIHAFLEKVPKTDEPSKMLGADFRIAAVRESWENALEKAGERSFSIVTL
                     KIVCGSGAYMRSLAGRIGAALGTKALALSIQRTKIGKYWMGFWLSSYS"
BASE COUNT         3335 a         3524 c         3513 g         3245 t
ORIGIN      
        1 agagcctcct ggggcaatag aaaagggaaa gcgggcgtga taggatagcg gaactgcttg
       61 gatagctcag ttggtagagc acttccctga agaggaaggg gtccccggtt cgagcccggg
      121 tccaagcaca cttatggcag ccttcggtac atttaaccgc gtgctcgcct ggagcgcccg
      181 taatgagcgc cccctcgggg ccgtactgtt tgccttcgga ttccttacgg acttcttcac
      241 gttcggtgtt cttccgatag ccgttgttaa ctatttcttt attgggtacc tctcccttgc
      301 ggctgtggca gctatcggcg cgcatgcctt tgctcgctac catgatgggg aaccgggcgt
      361 acgaaaagca cttgccgttc tattcccact tggcgtgcag tatgcgttcg gcggcctcct
      421 ctccggcttt gtcgttttct atactgcgca ctcggtggtg ctcgcgtcct ggccgttcct
      481 gatctttctt gcgctcgtgt acatcggaaa cgaatacttc cgcatgtaca agcactatct
      541 cgtgttccag acttcgctct tcttcttcac cctttatgcg tacgccatat tcggtctgcc
      601 gctcgtgata gggcgcatag ggccatggac cttcctaggg agtactgtgg ttgccatcct
      661 gttgtttgcc gcgttcctct gtctcctgcg gcttggaaac aaggcacgct ttgacgcaag
      721 cgttagacag atcgcactag cgtgcgtggg tatcgttgtt cttatatctg tttcctattt
      781 caccggagtt attccacctg tgccactcgt catgaaagat gcgggcgtct atcacagtct
      841 tgtaaaagtt ccgacggggt atcgagtgca agcagaagca cccaaggaat ggtgggatat
      901 acgtcctacc gtagtgcacg ttcttcctgg tgaggcgctc tacgcatata gcgcggtagc
      961 cgctcctatc tcattttcat caacggtagt gcatcgatgg gagcagtata tagacggcaa
     1021 aggatgggtg acacagagtc gcatttcgtt tccgctctca ggaggacgtg aaggcgggta
     1081 tagaggctat tcgataaaag atacacttac tccaggcaaa tggcgtatct cggtagagac
     1141 cgcaggcggc caggttatcg ggcgcgtacg ctttgatatt gaaaacgccg gcacggcgcc
     1201 ggcgctccaa gagataacgt tgtagctagt gaacgcgagc acgcttattc cacacggatt
     1261 caatagtggt ataagcagag gtttccatat ccctgcgctc gctctcaata ctcacatcgg
     1321 aaaaatctcg tgcttctaag ttacttttga ttgcgccgcg taagtgctgc atcagtgctc
     1381 ccagatcctc tctattcatg cggtttgctg catcacgtac cttatctaaa tagacatctt
     1441 ctgcgcgcac tgcctcagaa gcaggtgcgc cctcggtgtt gtatctaacg cgcgcgtggg
     1501 cagccaagtc tcgttcactc gtaaattcgc tcaagaatga atgtagcttc tccgcagctc
     1561 ctggctcgat agaggaggta gacccttcgt ctattgctag ctttaatttc tctccacttg
     1621 gctctaatcc ctcaagccct ttcataagtg attaactcga gcgcacctta agctttgtct
     1681 gacgtgtttt atcaagcttt ggaaggatga tcgtaagaag accatccttg gatgatgcct
     1741 gtgcgccatc aacatctatt tcctgtggaa gcataatggt gcgggcaaag gtgccccaga
     1801 agagttcctg attgaaatag tcaggacccg ttgcttcaga gcgttcttca cgggagccct
     1861 ctacgacgac catgtcgcgg ctgatggaaa cattcatctc gtcagggcgt actcctgcaa
     1921 caaatgtacg aataatgatt tcgctgccgg tctggtacat atcaaccgga agatgtcctt
     1981 cggtgcgttc ctcgtgcgcc ggatggggag ccgacatacc gcttgaaaca ttgaggtgct
     2041 ttcctccgcc tgcttccatc atgtcgtcat cgaaatcgta gtcgtcctca gaacgagttc
     2101 cggcaagtcg atcaaagagt gagcgctttt tcatatagct agcaggtgtt ttttggtaaa
     2161 ttgcggtaga ggaaatattt gagtatctcg aatgctgcga agatcgccac cgcgcccgtc
     2221 cacacggtaa agagcgcgag gatgacatac gagccatctc cttggattat aaaaaaatta
     2281 aacactgcat gcaatgcgat agcaaggata agtcctattg acgcagcgat agcacgcact
     2341 ggtcgcgatg atttatacga gaacgcgagt gcaaagccga tgatggaaga ggcaacgaca
     2401 tgaagaagcg ttgaaccaat gaagcgcagg ttcccaagac cgatagcttc aagcaagtat
     2461 ccctgagaga atggagcgag cagaaagagt gcgttctcaa gtgcggcaaa cccgagtgcg
     2521 acggtgagca tcgtagcaac gatatcgagc gagttacgca ccgcgtgacg gcgccacagc
     2581 acgaacgctg ctgctactgc gtatttagcg gtctcttcaa gcgctgacca tgcgataagg
     2641 actttagtgc caaggagggt cgtctctcca cccgagcagg caagtacgac acttatagcc
     2701 cactcacgac acgcaagggc ctgaagcggc atcacaatag gtactgctat catgccgcat
     2761 acaaaggcaa gcacgactgc ccagcgcggc tgcgggcagc ggtcttcttc cttaagaagg
     2821 aaatagagcc atgcgagagc cgggagcaac ccgcccacga gcgcataacc gatggtttcc
     2881 aaagagggca ttacccctcc agtataggcc atggagaggc cataagtagg gtgttagtac
     2941 cggtagatgc ccatatttct tcggtaatga acggcatgaa gggatgtaga agcgcaagac
     3001 agatctgaag gttacgcatc ataagcgctt tacgcgacgc aatgcgctca ggagggaagg
     3061 tacttggggt gtcgtgactt ccgaagatag gcttcgactc ttcaagaatt tcagctgcaa
     3121 accgatccca gatgtaatgg tagatggcgt cagcagcaag atacgcacgg tactcatcga
     3181 ggttcgtcgt tgctttcgct gctatctcat caagctcacg ggcaagcgcc tcgtcgtcag
     3241 cgtgaagtgg ggtagtaagg tccgcgtcgc cggcgttttc aagtacgaag cgtgcaatgt
     3301 tccatacctt attagcaaag tgcttatacc ccttcacctt atcctcgcgg agcgctaggt
     3361 cggtgccggg cgtgttacca accacgagcg ccatgcgaag cgcgtcggtt ccatacttat
     3421 ccgtaaggtc gagcgggtta attacgttac ctttcgactt actcatcttc ttgccgttcg
     3481 catcattcac gagtccatga aggtagacag tagggaacgg tacttggtcg gtgaggtaga
     3541 ggccaaagac catcatgcgt gcgacccaga agaagagaat gtcacgaccc gtttccatca
     3601 agtgtgttgg atagaactgg gagaaatctt tcccatcggg gtattccgtt gtaaggagcg
     3661 gccactgacc agaagagaac cacgtatcaa aggtatcagc atcctgcgtc cagccttctc
     3721 cgggtgactc aacacttgct ttccattcac cgtccttatg ccacgcagga atagggatac
     3781 cccaaacaat ttggcggctg atgttccagt cgagtgcgtt ctccatccag tggcggaacg
     3841 ttttctcttc gtattctgga ataaagcgta catcgccttt gttcacagcg gttatggcaa
     3901 gatttgcgag tggcgccatc ttcacgaacc actggtcttt tacctgcggc tcaatcatgg
     3961 cttggcactt gtagcacttc ttcacgacgt tcttgtaggc gtcgtccgta gacacaagga
     4021 gtcctttgct cgcgagtttc tcgacaatct ttccgcgtgc gtctttgata ttcatccctg
     4081 cgaattctgt agcgataggg aggagcttgc cgcgccagtc gataatctgc tccatcggaa
     4141 ggctgtgacg cttcgctatc tcgaagtcga tttgcgagtg ccatggcgta atggtcatca
     4201 caccggtacc aaactccatg tcagcagcgt tgtctttgat caccgttgcg gtaatagggc
     4261 cattgatcca ctccacctca agcgtgtcgc catgcttcca tttgctgtac cgcttatcgt
     4321 ccgggtgcat caccacgtac ttgtcgccga acttggtttc cgggcgcacg gtgccgatat
     4381 caaacggtcc atatttaaag gtgtagaacg gcgtcgtttc ttcaacgcta tcggtctcca
     4441 catcagaaag agacgtctga tgcttcgggc accagttcac tgtgcgtgct ccacgataca
     4501 ccaggtcatc gttgaacatc tttacaaacg tcgcctgcac cttctttaca agatcagggt
     4561 caagcgtgaa cttctcacgc gaccaatcgc aggacgctcc gagcgcgcgc aactgaccta
     4621 ccatgttctt cttattcgcg agagtgaagg taaggatttc ttcgtagagg gctttcgggt
     4681 ccataccaaa acgagaacgt ccttctttct cgagctgctt ctcatacact acctgtgttt
     4741 caaatcctgc atgatcagcg ccagggatcc acaacgcgcg atacccgcgc atacgcttga
     4801 agcgggtcac gatgtcctga agcgtcacaa agagcgcatg gccggcatgg aggttaccgt
     4861 tggcgttagg cggtggcatg acgatggtga atgccttacc ttccgtgggc gccagaagtt
     4921 ctgccgggag cttgtccgga ttgaaatacc cgctcttttc ccacgtctca tagatacgag
     4981 actcggtctc gccgggctgg tacggcttaa ggaatttgtc gtccattacc tagggaatat
     5041 agcaaatata tcgttttatt tcccgtcgcc ttgacggaag gcgcgcatag gtatgtagtg
     5101 ggggaactcg ctaatcaagg cgaggttgca ggaggcagaa atgcctatgg acgaaaaacg
     5161 aaaagctgaa gtaacgcggg cgctactccg tcttcgattg tgggagaacg ggctcaccct
     5221 ctcttcttct actaatcgaa aacttggcgc ttttgccaaa gaaagcggaa cgagcctgca
     5281 agagcttcga gagcttctta tagaattcct tccggacttc cttggagagg ctcttggggc
     5341 ggatttcgtc tcgatcagag tgggatatga cgacctggac ggcgatctca aaaaagttcg
     5401 tcattatgtc gacgctgagc acgaattcca ccgtcaagcg tcagcgtaag cagcacctta
     5461 ctaccgatcc tggttgggcc tcggtcctct agacgggtaa ttgcacctcc cgcgcacaag
     5521 cggcaagggc agaacaagaa ccgttccctc atcggtatac cggcggacag cactgtccgc
     5581 cggtattttt taagcaagcg aaagattacc gtcagctatg aaatctgctg aaaattcttt
     5641 acgagatgcg cgatagtagc cagcgatacc aatcatgacc gcattatcgg tagaaagtgt
     5701 tcgagcgggg atgcgaaggt caacgttagg atactcttct gcaatcttcg cttcaaattc
     5761 cttctgaata tgatggtttg cagatactcc gccgccgata acaaccgttt tcgcaccagt
     5821 tttgtctatc gcacgtgatg tcttggtcca aagcacatca gtgacggcat cttcaaacgc
     5881 gcgtgccatc tgttcctttt ctatatcggt aaggtcaccc ttgtcgcgta tttgatacaa
     5941 gactgatgtc ttaagtccgg agaaagagaa gtcaaaatca tcagtatctt tcatcgggcg
     6001 tgggagtttg aatggattct cgcgattctc tttacgatca cgctcagcaa gcttggagat
     6061 ctcaggcccg cccggatacg gaagtcccat catgcgcgcc actttgtcga atgcttcacc
     6121 tacggcatcg tcacgcgtcg cgccaaggtt ggtatacgtg agccagtcag tcatcgcatc
     6181 aagctcagtg tgcccgcccg atatgagaag accgagtatc ggaagctgag cattctcaat
     6241 ggtaatgaca ttaccttcac tctttccaag accagcgagg aagtgaccct ccatgtgatt
     6301 aattggaaca agcgggatat cccacgcagc ggcgagtgct tttgcgaagt taacgcccac
     6361 ccaaagcgct ggggcaagtc ctggcccatg cgtgatggca atagcgtcta ttgatgggcg
     6421 ctccgtagcg tcggcaaacg caataaacgc accaccaagt ccggtctctc gctcaagcag
     6481 ttcttgtatg cgaaccttct tttcttcaga aagcgtatgc gttccggtaa cgagaagacc
     6541 tgcttcttca agtgcgttac cgagaagtgg cgtaaggttt tctgcatgcg cgcgcttagc
     6601 aagggtgggg aacacaccgc catactcttt atgtatatct atctgtgaga gaagcgcgtt
     6661 tccaagtacg gtaaaggttg cggcgcttgc gtctcccgcg cactcaagga ccgctactgc
     6721 agtctcatcg cacgaggtct caatcgctag aactttcatc gtgttcaata acctcccaag
     6781 gaataggtgc gccccattct aacgaaaagg ctgcagatcc tggatgtcca tttccaccat
     6841 acttctgggc tagttctgcc aagttcacgg tcttattatc tcccgcacgc atagagacac
     6901 gcatcgagcc gtgtgcgtcg ttccgggtaa taagagtaaa cgcgccgcct tttttacgaa
     6961 gctgagtacc cagttcagta gcaaacattc gaggagcaga tatgagatac acctcatgtc
     7021 cttcaaatct gacgcgacgt gcgcgtgatg ctatctgggt agcgagaagc tggaaatatt
     7081 ctgcataggc tcgtccgcgt ccaataattt cagcacgact ctccggattc tctacacgct
     7141 cagtgagagt gtcccagcct gaaaaagagt atggttgcgc ataggcgtac gagataatgg
     7201 cacgctcatc atcagtgagc atctgatata aatcagcttt catcacaata tcgagaaata
     7261 ctggtacggg cgttttagga tgaaaatatg accaggcgat agtcgcgcct gaacgcttcg
     7321 catcgtatac atacgcaggc attgcttcga ccacatcctc aacaccctcg tggtgatcga
     7381 ggaccgttaa tgatgcagct tctgctgcaa gcacattcat aatgtctttc ggataacaga
     7441 aatcgataag gtacagatgt gctcccttga gacctgctgg tgtttctgct tgatgctgta
     7501 ctggtagata ttcagcagta tctccaaact ttttccaggc ggcataggca cccccaaacc
     7561 cgtcggggca attatcgtgg taaaggatta cggtgcgctc catagacttc acgataccac
     7621 taagcttcag cctttttctg ttcctgaagc gacgaccagt atgcggctcc cacgaacaca
     7681 agacccacaa gtccggtaat aacttcaggt acctgataga agaggcttgc aaacattgcc
     7741 gcagcaaggc caagaatggc ccagtgcgca ccatgttcaa gatagataag ggtatcaagt
     7801 gttttctgac gtacgaggaa cacggtaagg gtacgaacga agtaggcgcc gataccaaga
     7861 cctgcaacga tgaccgggag ctggctcgtt atcgcgaagg cgccgataac accatcgagt
     7921 gagaatgctg catcaagtac gttcaagtac acaaagagtg ctgcgcttcc agcagcgacg
     7981 gccttgccgg tttcaagcgt gaaggaattc gccacgccct gcatagcgat gaacaagatg
     8041 atgccgatga tgccagagaa gagaatcgtt gcggactgtg ccggcaacag aaacgccatc
     8101 accgcaagca aggtgagtgc tagaccgagc tcaagcgctt caatacgtcc gaaacgtgca
     8161 agcacacgct caaccgagcg aatccaatga atgtctttgc gttcatcgaa gaagtacttc
     8221 aatgacacca taaggaggaa cgcaccgccg aacgaggaga tagcgtagtg cgcgccttca
     8281 aggagttcgc cgtagtgctc aggatcaaag gcagcaagct gcgcaacagc ccaggggctc
     8341 atagcaactg aaaccgcgac gatgaatatc ggaagtacga agcgcgtgat cactacggca
     8401 aacagaatgc cccaggtaag gaagcgctgc tgccatttcg cattcatctt ggtgagcacg
     8461 cgcgcattca ccactgcgtt atcaaacgag agcgtgactt caagaatggt aaggaggacg
     8521 gcgagtgcga gtgcgtggaa accaccccat ataaatacac ctgcaagtag gagaatagaa
     8581 acgagcacgg gcaacacaaa gagttttgcg attgggttca tacgggtggg cgatagagga
     8641 ttcgaacctc tgaccctctc aacgtcaatg agatgctcta ccaactgagc taatcgcccg
     8701 tatgggtcgc agaatagccc gaaaagggct actattcaag cctatatgga gataattcga
     8761 ctcaccgagg ggaaccttat agaggctgcc aagaaggctg ctgccgtcct taagaagggc
     8821 ggtatcgtcg cgtaccccac cgacaccgtc tatggccttg gcgtatctat aacggaccct
     8881 accgccattg aacgtctccg caccctaaag ggaagggagc gaaagaagcc ggtctcgctc
     8941 attgttcaag atgtgcacca tatagagatg tgcgggacgc ttactccgct tgcagagaag
     9001 ttcgccgcat cacacctccc gggtgcgctc acgctcgtta ttcctgcaaa aaaagatatg
     9061 ccagagacac tcaccatgaa tggagcggtg ggagttcgcg ttccaaacga accattctgc
     9121 cttgcgcttg ccaaagaata cggaaagcca tacaccacga cctcagcgaa taaggcaggg
     9181 atgggaacgc cgcgtaacgt gcaaggattg atggagcact tcggtttgag cctcgatcaa
     9241 atagacctca tcattgatgg tggggaccga gccggaggca atccttccac cgttgtgtca
     9301 tgtgtgggag agataccgta tgtgttgcgt gaaggtgcgg tctcacgtca ggagctcggg
     9361 ttatagcgct ctaatcctag accgccagca cgtgataggc ttttgggtat gaaagaaatg
     9421 aacgagggtt gggagtcgtc agcgaggaac gttcaaagaa tcgagcaaat catatcgcaa
     9481 ccaattgttg aggaacaaat tgcacttcat gttcgaaaca taagagagat ccttctcaca
     9541 tcgaattctc gtaacgcaga aagcgattct aagataggaa aagttctaga ggactatgcg
     9601 cgacttatcg caatgaaatt cgaacaggtg aaggatatag aacctattgc tagctctgat
     9661 cctaatttag cagagacgtt aagcagtaaa acatttccta ttgccaagaa atatctcgaa
     9721 gcactcatgg atgggaagtt tctggctgaa cgcttgtcag acgatatggg gacaagcaag
     9781 gactggctca atgaagaggc agaatcagtg ttagctatga tagcggtgaa tatcatgcac
     9841 gatacgcttg ttgtagccta ctgtctgaga ctcggaatag atccagaacg gttgcgcact
     9901 agttccgcta catagtagtc tttcgatatg cctgaagaag cactccaatt catagcaact
     9961 cagcgtgtcg gtgtacttgc cgtccgtatg cctgacggca gtcctcatgc cgccaccatt
    10021 cactttgcgt atgttcctac cttaggaaag tatatttttc gcacttcacc tgagagtagg
    10081 aaagtagagt cagtgaagca gggagaaacc actggctcgt ttgtgattgg ctcaagcgaa
    10141 gcagagatga agactctcca aatggacggg gtagtgaagc ttgaagactc agaagagata
    10201 caagaggcgt accttggagg tcttaccgat aaatcaaaag aatctcccga tgtgtacctt
    10261 accttcaccc ctacctggtg gcggtacacc gactggacgc ttccccaagg aaagacaatt
    10321 tttagttcag acgcaaaata aaaacacccc gcatatgcgg ggtgttttta tagggcacct
    10381 aggtgccgtg ttcccagtca gctagatact tcttctgctc atccgtgagc tcatcgatag
    10441 tgatacccat tgaggcaagc ttggtgcccg ccaccatgtt ctcaatttcg tcggtcacgt
    10501 catataccgc tggggcgagc ttctcgcttt gggttgccat ccactcgcag gcaagcgcct
    10561 gagtagcaaa gctcatgtcc ataaccgacg ccgggtgacc ctctgccgct gctaggttta
    10621 cgaggcgacc atcagcaagg aggaagatac gattctttcc taccgtgtac tcgtccacga
    10681 atgggcgtac gttttcacga actgacgagg ctgccgcctt aagaccagca acatcaatct
    10741 caacatcgaa gtggcccgtg ttgcagatga tggcgccgtc cttcattgcc ttcatgtgct
    10801 ccatgcgaat gacatggatg tcgccggtag cggtgcagaa gacgtcaccc aacggtgctg
    10861 cttcctgcat ggtcatgacg cggaacccat ccatagtcgc ttcaatggct ttcaccggat
    10921 caatctcggt gacgataact tgtgcgccca tgccgcgcgc acgaagtgca aggccgcgac
    10981 cacaccagcc atagcctgcg gcgacgaata cttttccggc aagcaagatg tcggtagcgc
    11041 gcacgatacc gtccaaggtg gactggccag tgccatagcg gttatcaaag aggttcttgg
    11101 tctttgcgtc gttcactgca acgataggga atttcaacgc accgtccttt gccatagcac
    11161 gaagacgaat gacaccggtg gtggtctctt cagtaccgcc gatgatggag tcaataagct
    11221 gtgggtagtc tttgtgaatg gtagagacca gatcagcacc atcgtccatg gtgaggtgtg
    11281 ggttctcacc aatgacggcg tttaagtgtg agaagaacgt atcgcgatct tcgccttttt
    11341 ttgcgaacac tggaatacct tcatcaacga caagtgaggc tgcgacatcg tcctgcgtgg
    11401 agagtgggtt agaagcgcag agtacgacgt ttgcgccacc cgccttcaag gtgcgcatca
    11461 aggtaccggt ctctgccgtc acgtggaggc aggcaccaat ggtcttacct ttaaatggct
    11521 gctccttagc aaagcgctca cgcactttcg caaggacggg catatcgtta tctgcccatg
    11581 caaggcgctt cttcccgaga gccgctaagg actcgtctgc aatatcgtgc ttcatacgtt
    11641 agataggaaa agattcgtta taagcgccct cgtaccttgc tttcaactcg tcgagcgtaa
    11701 agcggtggtt ctgagtacca tagcactcca ccgcgtacgc ggctaccgta cttccaagct
    11761 ttgcgcatac gggaagagga agctcacgca catatcctgc aaggaagcca ccacgaaacg
    11821 catctcccgc accggtaggg tcaactactg tggtaacggc taccgctgca actcgttcct
    11881 cactgttttt ggtaataatg cgtgaccctt ctgcgccaag agtgacaata aggaatccta
    11941 cctgttccgc tatcgctgcc tcattccatc cggtcttctc gccaataaga gagagttcgt
    12001 aatcgtttac taacattcct gcagaatcgg ttatcgcttc acgtagctcc tcagcagaga
    12061 gcataggaat ttgctgcccc ggatcaaaga agaagggaag actagcggct ttgctctctc
    12121 ttgctatacg caccatgtca tctttattgg ttggtgacac aatagcggcg ctatagtcgg
    12181 catacgcaat agcattggta tacgcctgca tggcagcgct tccggagaat gccgttatct
    12241 gattatcttt cttgtccgtc atgatgtagc cggacgccgt cggaacgtcc ttgacgcgcg
    12301 ctatggttgc aacgcccacc tgatgatcag cgagccatgc gccgtatcgg tcaaagtcat
    12361 ttcctgcagt tgcgacaacg tctgcctttg tctcaagaag ggagagagag tacgcgatat
    12421 tacctcccgt gcctccgaag ttttctttcg gaggagacac aaagaaactc acgcttatcg
    12481 agtgcagttt atccggcatg aaatgctcgg aaaagttgcc atcgaaatcc atgatccggt
    12541 cataggctat agatccacac acaagtatcg gtttcattgg ggccagtgta cctcatatat
    12601 actcctcgta tgaagcgata tgtcgtactc gataaaaagg taggggagac tccccttaga
    12661 gctctcaccg actggcagaa cgcacacccg aactatgccg gtatgcccgc aagctatgcg
    12721 ggacgccttg accccatggc aagcgggaag ctcctcatac ttcttggaga agagtgtaag
    12781 aagcagaaga agtacatcgg acttgataaa gagtacgtga tagaagtttt gcttgatgtc
    12841 gggagtgata ccggggatgc actcggtctc atcgactacc caaatagaga cacacgctcc
    12901 aacgattctt ttttaacaaa ggtacttcat gcggagcgtg gtacccacaa gcgtgcctac
    12961 cctgcattct cttctaaaac ggtgaacggg aaaccattat ttctgcacac actcgagggt
    13021 tctataaaag atattgctat accagagcat gatgaacaca tctatcgatg ctcactgctt
    13081 cgtgcagagc gtatccatgc gtccgcactc aaggctcgta tacacgcttt tcttgagaag
    13141 gtaccgaaga ctgatgagcc atcaaagatg ttaggtgctg actttcgcat tgcggcagtg
    13201 cgtgaaagtt gggagaacgc acttgagaag gcaggggaac gaagtttctc catcgttacg
    13261 ctcaaaatag tctgtggctc aggtgcttat atgcgttctc ttgccggacg cattggagca
    13321 gcgcttggca caaaggcgct cgcgctttct atacaaagaa caaagatagg gaagtattgg
    13381 atgggattct ggctcagttc ctattcataa tcttttcact cagataggtc atactgtgag
    13441 gatgcatacc actaagcagc tcgtcatcgg cgtgctcgtg ctcctcatcg taggaattgc
    13501 cggatttggg atctatacct attacaaaga gaagcctcct gcgtggctca tcaagttggt
    13561 aacgcacacc gcgccaccgc cgccactccc cgaagggaat gtcgcaccat tccaggc
//
LOCUS       DAORIN010000008        12213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_884_length_12213_cov_4.853759, whole genome shotgun sequence.
ACCESSION   DAORIN010000008 DAORIN010000000
VERSION     DAORIN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12213
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_884_length_12213_cov_4.853759"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PK109_00650"
     CDS             <1..491
                     /locus_tag="PK109_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24087.1"
                     /translation="LAVALVGPYTAVQHALSASYVARDELAASALAQEGMEYIRSVRD
                     NNYLNTSPTRTWMTGLSTLSCYGLTPSGYCTIDPTLGDVNTTPAAIAAYGTLANVPVL
                     YITSTGLYNQQTLGTPTRFKRSMQIKKISETEVQVTITVSWISVRQSYSVVVTDNLQD
                     WL"
     gene            488..946
                     /locus_tag="PK109_00655"
     CDS             488..946
                     /locus_tag="PK109_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HRH24088.1"
                     /translation="MIHYPHKGFALLISVVLAAVALSIGLALLDIALKQVILASAAKQ
                     SQKAFYAADSVMECALYYDQQLNAFDRTNNISAITCNTLPVTNYLSPAPSGSDPRVTT
                     FTVPCAGSGEQGSVTVYKNSDASTQIYALGYNTCDTNNTRRIERGLKVTY"
     gene            949..2964
                     /gene="ligA"
                     /locus_tag="PK109_00660"
     CDS             949..2964
                     /gene="ligA"
                     /locus_tag="PK109_00660"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRH24089.1"
                     /translation="MKIPKEVEERVRALQAEITRLRTLTHVRDTAEISPEALDSLKHE
                     LATLEREYPELVTPESPTQRVSGGVFPELKKVRHAIPQWSLDDAFDEAEIRAFDERVR
                     RGLERALGRAVTPTYVCELKIDGAHLILTYEKGQLVQAATRGDGVVGEDVTHTVSTIQ
                     GLPKTLRAPRSLIVEGEAYMSKKGFAELNARRAKEGEAQFANARNVVAGSLRQLDPAA
                     AASRPLGTFLYDLEYLDESMPPTQAEELEFLRSVGLPVNRHEKKVATVEEIVAYWKEW
                     NGARREAEDYLIDGVVVKVNERAYQEALGHTGKGPRYAIAFKFPAEQVTTVVEDITLQ
                     VGRTGKLTPVAHLRPVAVAGTTVARATLHNEDFIREKDIRIGDTVILQKAGDIIPEIV
                     EVLPALRPASAKAWRFPKKSPLCGGDGSIERIPGEAAHRCASAGSFLVQERKLAHFVG
                     KSALDIDGFGKKTVALLMQHELLSEFDDIFDLTRDELLALPGFKDTSADNLIVAIRAA
                     RTVPLDRFLVGLSIDHVGEETAYLLATNFSTLKKLSSASADTVAAIKGIGEVVGNAVA
                     AWFAHEENKKLIERLEHHLTFEKVVAPAKGPLTGLTVVVTGTLPTYSRDEAESLVRRA
                     GGSVAGSVSKKTSFVLAGEAAGSKLSKAETLGVEVIDEGEFKRRLGL"
     gene            2978..3268
                     /gene="gatC"
                     /locus_tag="PK109_00665"
     CDS             2978..3268
                     /gene="gatC"
                     /locus_tag="PK109_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRH24090.1"
                     /translation="MASTDDVKKLAALARLSIPEHDLARFSKEFESILTYISQINELK
                     VSKEAKAVPTMRNVFREDGEPHATGLYTEKIAEQFPQRDGEYLSVKQIITHD"
     gene            3261..4691
                     /gene="gatA"
                     /locus_tag="PK109_00670"
     CDS             3261..4691
                     /gene="gatA"
                     /locus_tag="PK109_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRH24091.1"
                     /translation="MTKPLNELTIQEAAKGLRAKEFSVRDLWDACVKVAHEKNPELNV
                     YLEIFDADDAAISAAQKRIDEEGENAPLLCGIPLAMKDNILIEGKIASAASKILENYT
                     ATYDATATRKLKEAGALFVGRANMDEFGMGTSTENSAYGPVRNPHDVSRVPGGSSGGS
                     AAAVAAHMALGAYGSDTGGSCRQPAAYCGVVGFKPTYGAVSRHGLIALGSSLDQIGEM
                     ANSVADVRALYEVVKGHDPLDATTIASTTYPERAVPTTFRIGVPYDLLEGIDAKVKTR
                     FDETLETLKNAGHEIVPVALPLSGLALPMYYIVLPAEISANLARFDGIRYGVAKRGDT
                     LLDDYLGSRTEGFGAETKRRILIGTYVLSSGYIDAYYRKADAGRDMVRAEYNAAFEQV
                     DVVAVPTAPSVPFKFGAKSDPVALYLEDIFTVTANLTGMPAISVPMEMIEEDGVALPL
                     GIQFTAPHGAENTLFAIGAAVTGETL"
     gene            4701..5309
                     /locus_tag="PK109_00675"
     CDS             4701..5309
                     /locus_tag="PK109_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24092.1"
                     /translation="MENGTTGPIHFLNLEYFFRLLYESRVGITGSSTPITLFDGFIEW
                     VTHTWSVLGTVSFVFSLVAFAILAYSTVRMFQIKEREEHERWSTLDMSEAEKEKDHSR
                     WAHIQALIESPQERDWHEAISEADIMLDDLLEERHYAGETTEERLAKADPATFKTLTE
                     AREAHQLRIDMLLGGPDFKLDDHTAYRTIKKYELVFKEFGEI"
     gene            5348..5418
                     /locus_tag="PK109_00680"
     tRNA            5348..5418
                     /locus_tag="PK109_00680"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5380..5382,aa:Met,seq:cat)
     gene            5544..6335
                     /locus_tag="PK109_00685"
     CDS             5544..6335
                     /locus_tag="PK109_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRH24093.1"
                     /translation="MSETIVVYHRCGGKSLNYPPNTILTAQWAQAHGARAIEYDVVLA
                     QDGDEPKMVVMEPKVIKEAGLDINNLRWEDVRNLDAGNATFGFQKIPTLEEMLTAIDA
                     SKTGHQIQLKGQHPDSVKVLLSKVTNLNSFVITAFDISVIQEIKSFDKTIPVGWLVKP
                     QQEGGDEAGVDLTAKLVADIGSIRPYSEEEIESIVKTAKENTIEVILLCGPRINSREM
                     VQKFRSGGSQVGAWGVATNLALAKQLIEFGLDRFTLDNPEELALV"
     gene            6584..7207
                     /locus_tag="PK109_00690"
                     /pseudo
     CDS             6584..7207
                     /locus_tag="PK109_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016705.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
     gene            7345..7917
                     /locus_tag="PK109_00695"
     CDS             7345..7917
                     /locus_tag="PK109_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRH24094.1"
                     /translation="MDASIPSDEALMAQVQQGNAEQFGILMKRYEPKLMRYGSRFLAK
                     SDDIREIVQDVFIKTYQDSKSFDTSQRFSPWIYRIAHNAFANELRRKSRSPLQLPDFD
                     VLLSHAPARESTDSESERASLVRMVEKGLESIAPKYREVLVLYFMEELSYKEIADVLR
                     VPVSTVGVRMARAKAALKAVYSAQHLSYEP"
     gene            7907..8629
                     /locus_tag="PK109_00700"
     CDS             7907..8629
                     /locus_tag="PK109_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24095.1"
                     /translation="MNPNSPQKEIQSEVLERIRSGELRMKPRLFFILSTALVVVIMAL
                     ILVVSIGLASFISFGLRLNGHESLLGFGTRGPLLFLAVFPWGLALIDLALIAVLAWLL
                     RRFKFGYRKPLLILVAVLVVGAAAIGLGIDRESRFHDDRYEEAEAGELLGPLETLYES
                     ARMSAPEEYGIYRGFVISKEDDSFMLTHDDHDNDEDDGTWKVYSPPGFVMELLQLGDR
                     VYVAGEREEDGIEAYGVRILER"
     gene            8670..8864
                     /locus_tag="PK109_00705"
     CDS             8670..8864
                     /locus_tag="PK109_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRH24096.1"
                     /translation="MPTQTLAYSLYVCLECGWIYDETQGMPERGIAPGTLWASLPDDF
                     QCGDCDIQKRDVHMWQRLPA"
     gene            8861..9208
                     /locus_tag="PK109_00710"
     CDS             8861..9208
                     /locus_tag="PK109_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24097.1"
                     /translation="MRKDTKTFLTTVLGIPLVLSGAWGVNTANQAGVVVYEREAVAED
                     VPEVVVVPEVKEETPPPVTVPAPKPVPVAVTPPPVVPAPPPPAPVVVATVTPPVAPAP
                     KPVVKKSRRTRAS"
     gene            9213..10139
                     /locus_tag="PK109_00715"
     CDS             9213..10139
                     /locus_tag="PK109_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRH24098.1"
                     /translation="MQEFDYEKNVMGSEASISIVTDSRAHADATAATLFAIAEREEAR
                     FSRFRPESELSLLNKERSRSVSPECLETVLLGRDLYRASSGLFNPLVAIARFGYDADI
                     AEVKGRDRTGNSHDAYSIDMESVGIDEKTRTVFLQEGQTLDTGGYTKGHTAQLMAEAA
                     VGCQGVLVNLGGDIYARGLDAEGKPFVFHIDNPKDSSMDLSFFVTNAGIATSGSYNRT
                     WMLEGKPFFHILDASGTKNPVTDIVSATVIAPSGAEADAFATVALVAGVQAAECLLEE
                     EGYEYCFITTNGSIIFSGAFPLVENAESYLYV"
     gene            10132..10758
                     /locus_tag="PK109_00720"
     CDS             10132..10758
                     /locus_tag="PK109_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24099.1"
                     /translation="MSNKAYFAFIAWFQEALLALGIGILIVLPVMLAYLPEYLPDWSY
                     TTLFTISLVAVFLVMMIRPLADLFPNVPWLRPLVILRKGFGVLSASIIVGIMFSKFMV
                     DGVSYVIDFFSPERWSLAEGAILAPLADLSALLLLITSNKFSKRVLAKNWKRLQKLAY
                     VYFYAGALYEYVLLGQAFALVAMVLVTIVVVAAFVKNRWAPAASLQAV"
     gene            10755..11456
                     /locus_tag="PK109_00725"
     CDS             10755..11456
                     /locus_tag="PK109_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRH24100.1"
                     /translation="MTIKRMPATIGTITDLSPTAREVTLHLPELLGFTPGAFVNVFMD
                     HDGTRMRRAYSISSDETAQDMIALSIRKGSVGGMSERFWDTGVAEVPLQIMGPLGLNT
                     ADKITGTRVFLFAFGIGVSVIKGLLPHLLRRRDITHITVMTGSRNEEEALYKEFFEEA
                     ARRDPRLTVTMVLSKPHDTAYPYKGHLQDAIGGIDFTDASVYICGTKAAAESLQTAIT
                     KKGQPVHQFLIEAFD"
     gene            complement(11459..12196)
                     /locus_tag="PK109_00730"
     CDS             complement(11459..12196)
                     /locus_tag="PK109_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009916 - alternative oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HRH24101.1"
                     /translation="MESTAPSFQMNDDYVAHEKLVQELNDPKLREEYAQPLNRYRTGI
                     IPGILGWLLVTAGNTFYGKRPSYLKFKAVEVIARIPYQSWESAAYTMLTAFYGNEKHA
                     IRLCRMAAFGRFAQDNETMHVVVISSLVRKKGRDAFFRHTLVPLLFSFFYFWVVYLVY
                     IFSHRAALELNYLFESHAYHQYQQFLDEQGESLKQIPVVSDFLDFYGRNVRNEYEFFE
                     SVRNDELIHRNRSIREIESHKRVWQEK"
BASE COUNT         3038 a         3045 c         3288 g         2842 t
ORIGIN      
        1 tgcttgccgt agcactcgtt ggtccctaca ccgcggtgca gcacgcactc tcggcgtcct
       61 atgtggcgcg cgatgagctc gccgcttcag cgttagcgca agagggaatg gagtatatac
      121 ggagcgttcg tgacaataac tatttgaata caagcccgac gcgcacctgg atgacgggtc
      181 tttcaacact gagctgttac ggcctcacgc caagcgggta ctgcaccatc gacccgactc
      241 ttggcgacgt gaacactact cctgcagcga tagcagcata cgggacgctc gcgaatgtcc
      301 ccgtgctcta tatcaccagt acggggcttt ataaccagca aacccttggc acaccgacgc
      361 gtttcaagcg ctccatgcaa attaagaaga taagcgagac tgaagtacag gtaacgataa
      421 cggtgtcctg gatatccgtg cgccaatcct actcagtcgt cgttactgat aacctccaag
      481 attggctatg atccattatc ctcacaaagg gtttgcgctc cttatctcgg tggtactcgc
      541 tgcggttgcg ctttctattg gccttgcgct tctcgatatt gccttgaagc aagtcatact
      601 tgcgtccgca gcaaaacaat ctcagaaagc gttttatgcc gctgactcgg taatggaatg
      661 cgccctctac tatgatcaac agctgaacgc gtttgatagg acgaacaaca taagtgctat
      721 tacctgcaac accctcccgg tcactaatta cttatcaccg gctccgtcgg gtagtgatcc
      781 gcgcgttact acctttaccg tgccctgcgc tggttctgga gagcagggga gcgtaacggt
      841 gtataagaat agtgacgcat cgacccaaat ctacgccctt ggctataaca cctgcgacac
      901 caataacacc cgtcgcatcg aacgcggact caaggtaacc tattaggaat gaagattccg
      961 aaggaggtag aagagcgcgt ccgggcgctc caggcagaga ttacgcgttt gcgtacgctt
     1021 acgcatgtcc gggacacggc tgagatctct cctgaagcac ttgattcctt gaagcatgag
     1081 cttgccaccc tggagcggga gtaccccgaa ctcgtcacac ccgagtcccc aacacaacga
     1141 gtgtcgggag gcgtctttcc tgaactaaag aaggtgcgtc atgcgatacc gcaatggtcc
     1201 ttagacgacg cctttgatga agcagagata cgtgcgttcg atgaacgggt gcgtcgcggt
     1261 cttgaacgag cactcggacg cgcagtaacg cctacctatg tgtgtgaact taagattgat
     1321 ggcgcgcacc tcattcttac ctatgagaag ggacaattag tacaagcggc aacgcgtggt
     1381 gatggggtgg tgggtgaaga cgtcacgcat acggtctcta ccatacaagg acttccaaag
     1441 acgctccgcg cgcctcgcag ccttattgtt gaaggagagg cgtacatgtc gaagaaagga
     1501 ttcgcagaat taaacgcacg tcgtgctaaa gaaggagagg cgcagtttgc aaacgcacga
     1561 aatgtggtcg caggctccct gcggcagctt gacccggcag cagcagcatc gcgcccgctt
     1621 ggcaccttcc tctacgatct tgagtattta gatgaaagca tgcctccgac acaggcagaa
     1681 gaacttgagt ttcttcgctc tgtcgggctt cccgtgaacc gacatgaaaa gaaagtggct
     1741 accgtagaag agatagtcgc gtactggaag gaatggaatg gtgcgcgtcg cgaggcagag
     1801 gactatctta tcgatggagt agtggtgaag gtgaatgaac gtgcgtatca ggaagcgctc
     1861 gggcacaccg ggaaaggtcc gcgctacgcc attgccttta agttcccggc agagcaggtg
     1921 accaccgtag ttgaagacat caccctccag gtcggaagaa cggggaagct cactccggta
     1981 gcgcacctgc gtccagttgc ggttgcgggc actacggtag cgcgtgccac gcttcataac
     2041 gaagatttca tacgtgagaa agatatccgt atcggggata cggtcatctt gcagaaagcc
     2101 ggtgacatca ttccagagat tgttgaagta cttccagcgc tccgtcctgc gtcagctaag
     2161 gcatggcgct tcccaaagaa gtcaccgctc tgcgggggag atggatctat cgagagaatt
     2221 cctggagaag cagcacaccg ctgtgcctca gctggatcgt ttctagtgca ggaacgtaag
     2281 cttgcgcatt ttgtggggaa gagtgcgctt gatattgatg gatttgggaa gaagacggtg
     2341 gcgctcctta tgcagcatga gcttctttca gagttcgatg acattttcga tctcactcgc
     2401 gacgagcttc tcgcgctccc gggctttaaa gacacatctg cggataactt aattgtggcc
     2461 atacgcgccg cacggaccgt accacttgat agattcctcg tcggcctttc tatagatcac
     2521 gtcggagaag agacggcgta ccttcttgca acgaacttct ccactcttaa aaaactttcc
     2581 tctgcgtccg ctgacactgt cgcggcgata aagggaatag gggaagtagt cggaaacgcg
     2641 gtcgctgcat ggtttgctca tgaggagaat aaaaaactca tagagcgcct tgagcaccat
     2701 cttacgtttg agaaagtcgt tgctcctgca aaaggaccac tcaccggtct tacggtggta
     2761 gtgaccggta cgctccctac ttattctcgc gatgaggcgg aatcgttggt gcgccgcgca
     2821 ggggggtcag ttgccggatc ggtctccaaa aagacaagct tcgtgctcgc aggagaggct
     2881 gctggcagca aactttctaa ggcagagacc cttggggtag aggtcattga tgagggggag
     2941 ttcaaacgaa ggctcggctt gtgatagagt ggcgggaatg gcctcaaccg atgacgtaaa
     3001 aaagcttgcc gcccttgcac ggctctctat accagagcat gacctcgccc gcttctcgaa
     3061 agaattcgag agcatactta cgtatatcag tcagataaac gaattgaagg tcagtaagga
     3121 ggcaaaggca gtccctacga tgcgaaacgt gtttcgtgaa gatggggagc cccatgcgac
     3181 ggggctctat acggaaaaga ttgcggagca atttcctcag cgtgacgggg agtatctttc
     3241 cgtgaagcag attatcaccc atgactaaac cactgaacga gctgaccata caggaagctg
     3301 caaaaggact tcgtgccaag gaattttccg tacgtgatct gtgggatgcg tgcgtgaagg
     3361 tggcgcatga gaagaaccct gagctcaacg tctatctaga gatatttgac gcagatgacg
     3421 cagcgatatc cgctgctcag aaacgtattg acgaagaagg tgagaacgcg ccacttctct
     3481 gtggtattcc gcttgccatg aaagacaaca ttcttattga gggcaagata gcaagtgctg
     3541 catcgaagat tcttgaaaac tatactgcta cttacgatgc gacagctaca cgaaaactta
     3601 aagaagcggg agcgttgttt gttggacgcg caaacatgga tgagtttggc atgggtacgt
     3661 ctacagaaaa ctctgcgtac gggccagtaa gaaaccctca tgatgtttcc cgcgtgcctg
     3721 gtggttcttc aggaggatcg gctgctgcag tggcggctca tatggcgctt ggagcgtatg
     3781 gatccgacac tggaggatcg tgtcgtcagc ctgccgcata ctgcggcgta gttgggttca
     3841 aaccaacgta tggtgccgtt tcgcgccatg gacttattgc gcttggatca tcacttgatc
     3901 agatagggga gatggctaat agcgttgcag atgtgcgagc gctctatgaa gtagtgaaag
     3961 ggcacgatcc gcttgatgct acgaccatag cgtctactac atacccggaa cgtgcggtcc
     4021 cgacaacatt tcgcatagga gtgccttatg atcttcttga aggaatcgat gcaaaagtga
     4081 agacccgatt cgacgaaaca cttgagaccc tcaagaatgc tggtcacgaa atcgtacccg
     4141 tcgctctacc actctccggg cttgcgctcc cgatgtacta cattgtgctt cctgctgaaa
     4201 tttcagcgaa cctcgctcgt ttcgatggta tacggtatgg agttgcgaag cgcggcgaca
     4261 cacttcttga tgattacctc ggaagtcgta ctgaaggctt tggcgcagaa acaaagcgac
     4321 gcatccttat tggtacctac gtactctcgt cgggatacat tgatgcctac tatcgaaaag
     4381 ctgatgcggg acgggatatg gtgcgcgcag aatacaatgc tgcgtttgag caggtagacg
     4441 ttgttgcggt tccgactgcg ccatcagtac cgttcaagtt cggtgcaaag tcggatccgg
     4501 tggcgctcta tcttgaggac atctttacgg tgaccgcaaa cctaaccggt atgccggcca
     4561 tctccgtgcc tatggaaatg attgaagaag acggggtagc gcttcctctt ggcatacagt
     4621 tcacggcgcc ccatggagcg gagaacacct tattcgctat tggtgctgca gtgaccggag
     4681 agacgctata atcaaaggta atggagaacg gtaccaccgg cccaatacac tttctcaatc
     4741 ttgagtattt cttccgtctc ctctatgaga gtcgggtagg aataacggga agctcaaccc
     4801 ccatcaccct cttcgatggg tttatcgaat gggtaacgca tacctggagc gtgcttggca
     4861 cggtatcgtt cgtgttctca ctcgttgcgt ttgcgatact cgcatactcc accgtgcgca
     4921 tgttccagat aaaggaacgt gaagagcatg agcgatggtc cacacttgat atgtctgagg
     4981 cggagaaaga aaaggatcat tcccgctggg cccatattca agcactcatc gagagtccac
     5041 aggaacgtga ttggcatgaa gctatatctg aagccgacat catgctcgat gatcttttgg
     5101 aagaacggca ctatgcggga gaaactaccg aagagcgtct tgcgaaagca gacccggcaa
     5161 ccttcaagac gcttaccgaa gcgcgtgagg cgcatcagct ccgcatcgac atgcttctcg
     5221 gtggtcctga tttcaaactc gatgaccata cggcatatcg caccattaaa aaatatgagc
     5281 tcgtgttcaa agagtttggc gagatttaag ttgcaaaagg caataaaaac cgtatagtat
     5341 aggccatagc ggggtagagg agaggtacct cgggaggctc ataacctcca gacgccggtt
     5401 cgattccggc ccccgcaaca cgattgtgat agtggaactg agaccggttc ccgtcgaaag
     5461 acggttctgt accggtccgt gtcaacagca taaaaggcgc gaaagcgcct tttatgctgt
     5521 ctgaatgaag ctataattct tgcatgtcgg aaacaatagt tgtttatcat cgctgcgggg
     5581 ggaagtccct taactatccc ccgaatacga ttctcaccgc acagtgggcg caagcacatg
     5641 gtgctagggc gattgagtat gacgtcgtgc tcgcgcaaga cggtgatgag ccaaagatgg
     5701 tcgttatgga gccaaaagta ataaaagaag cggggctcga cataaataac cttcgctggg
     5761 aagacgtgag gaatcttgat gcgggcaacg caactttcgg ttttcagaaa ataccgacgc
     5821 tcgaagaaat gcttacggct atcgatgctt ccaagacagg acatcaaatt cagctcaaag
     5881 gccagcatcc cgacagcgtc aaagtacttc tctctaaggt cacaaacttg aacagcttcg
     5941 tcattacggc ttttgatatt tccgtgattc aggagataaa aagttttgat aaaacaatcc
     6001 cagttggttg gcttgtaaaa ccacagcaag agggaggaga tgaggctggc gttgatttaa
     6061 ctgcaaagct tgtcgctgac atcggcagca tacggccgta ctcagaggaa gaaatagaaa
     6121 gtattgtgaa aacagcaaag gaaaatacga ttgaggtgat tctgctctgc gggcctcgca
     6181 taaatagtcg tgaaatggtt caaaagttta gaagtggagg atcgcaagtc ggagcatggg
     6241 gagttgctac caatctagct cttgcaaaac agcttataga attcgggctt gatcggttta
     6301 ccctcgataa tcccgaagaa ttagctctcg tataatgagt tctataccgg cccgcctccc
     6361 agtcactcga aaatcgtcaa aactttcaaa atcacatcta gaccggtccc catcaaccaa
     6421 cgtccgaaaa gtgtgccaaa agaaccctta gaacctctcg agttccgggt actgtaccga
     6481 cacgggcaac aagaaaaagg ttccccagaa aagcatctct ttgagatgct tttctgctat
     6541 agagtgaaaa caatgccgta agagtgagtg ctaggatgta agcatgtatt acctaatcgc
     6601 gatcattgta gtgctcgtcc tgtggtcggt ggtaggcgct gtctcctcgc gcgcagaaca
     6661 gacgccatat accgtaacga gggtagagaa ggggtatgaa gtgcgcacct atccttctca
     6721 tatcgtggcg caaaccacgg tatcgggacc atatgatcgc gcccttaacg aaggatttcg
     6781 catcgtagcg ggatatatct ttggaggaaa cacaaagaaa gaaagtatcg ccatgacagc
     6841 acccgttatt gagtagaaga cgaaaggcga gacgatagcc atgaccgctc cagtcacggc
     6901 aaccatagag ggagaacaac acaccattgc cttcgtgatg ccgcgctcgt atacactggc
     6961 aacccttccg gtgccaacag attcacgagt caccctggct acggttcctg aaacgaccat
     7021 ggcagttatg cgcttctcat gggttcgtac taacagccgt gtcgaagaaa agaagcagga
     7081 actgcttaag gcactcgcgc gcgataccgt aaccgtgacg ggagaagtgc agtacgcggg
     7141 gtataacgca ccctggactc caccgtggat gacccgaaat gaggtcatgg tgcctattca
     7201 actctagtac gaaatattct ccgcatatgc ggtgtactac gtgcgtggcg cagatacagc
     7261 cattaccacg taacctttcc tttcctatga gcgatatcag gctcacttct ttgcggagag
     7321 gtgggtctgc gcgatactga ggatatggat gccagcatcc cttcagatga agcgctcatg
     7381 gcgcaggtgc agcagggaaa tgctgagcaa ttcggcatcc ttatgaagcg gtacgaacct
     7441 aaactaatgc ggtacggatc acgcttcctt gcgaaaagcg acgatatacg cgaaatcgtg
     7501 caggatgtgt ttatcaaaac ctaccaagac agtaagtcct ttgatacgtc gcagcgattc
     7561 tctccctgga tctaccgtat agcgcataac gcatttgcta acgaattacg tcgtaagagc
     7621 cggagcccgc tccagctccc tgactttgac gtgctcttga gtcatgcgcc ggcacgcgag
     7681 tccaccgata gcgagagtga gcgggcgagc cttgttcgca tggtagagaa aggactagag
     7741 agtatcgcgc caaagtatcg ggaagttctg gtactgtatt tcatggaaga gctctcgtat
     7801 aaagaaatcg ctgacgtact tcgtgtgcca gtaagtaccg tcggtgtgcg catggcgcgc
     7861 gcaaaagcag cgctcaaggc agtctatagt gcacagcacc tatcctatga accctaactc
     7921 accccagaaa gagatacaga gcgaagtgct cgagcgtatt cgctcggggg agcttcgtat
     7981 gaaacctcgc ctctttttta tactatcgac agcactcgtg gtagtaatca tggcgttgat
     8041 actggtggta tctatcggac tcgcaagctt tatctctttc ggactccgat taaacggcca
     8101 tgaatcgttg ctcgggttcg ggacccgcgg accccttctc ttccttgcgg tatttccgtg
     8161 gggccttgcg ctcattgatc ttgcgcttat cgctgtactt gcctggctcc tgcgtcggtt
     8221 taagtttggg taccgaaagc cgctcctcat actcgttgcg gtactagtgg taggtgcagc
     8281 tgcaatagga cttggaattg atcgcgagtc gcgatttcat gatgaccgat atgaggaagc
     8341 agaggcaggg gaactcctcg ggcctcttga gaccttgtac gaaagtgcgc gcatgagtgc
     8401 tcccgaagag tacggcattt atagagggtt cgtaatctca aaagaagacg actcctttat
     8461 gcttacgcac gacgaccatg ataacgacga agacgacggt acgtggaaag tgtattcgcc
     8521 gccggggttt gtgatggaac tactgcagct gggtgaccgg gtgtatgtag caggggagcg
     8581 ggaggaagac gggattgaag catacggagt gcgtattctc gagcgctagc agggtgaaat
     8641 aaagggctag gtaccggtgt agtactggta tgcctaccca aaccttagcc tactcgctct
     8701 acgtgtgtct tgaatgtgga tggatatatg acgaaacgca aggcatgccc gagcgtggta
     8761 ttgcgcccgg caccctctgg gcaagcttgc cggatgattt ccagtgcggt gactgcgata
     8821 tccaaaagcg cgacgtacat atgtggcaac gccttcctgc atgagaaagg atacaaaaac
     8881 attccttact actgtccttg gcataccctt ggtattaagt ggtgcttggg gtgttaacac
     8941 tgcaaatcaa gcaggagtcg tggtctacga acgcgaagca gttgcggaag atgttccgga
     9001 agttgtagtg gtgccagagg ttaaagaaga gacacctccc cctgtaacgg ttccggcccc
     9061 caaaccagta cccgtagcag tcacgccccc accagtagta cctgcacctc cgccccctgc
     9121 gcctgtggtg gtagcgacag taacgccgcc agtagcgcct gcgccaaagc ctgtcgtcaa
     9181 aaaatcgcgc cgcacgcgtg cgagctaata ccatgcaaga gtttgactac gaaaagaacg
     9241 ttatgggttc agaagcgagc atcagcatcg ttactgattc gcgcgcacat gcggacgcca
     9301 ccgctgctac gctctttgca atagcggaac gagaagaggc acggttctca cgctttcgtc
     9361 ctgagagcga gctatcgcta ttaaataaag aacgttcacg cagtgtttca ccggagtgtt
     9421 tggaaaccgt tcttctcggg agggacttgt atcgtgcgtc ttcaggacta tttaacccgc
     9481 tcgttgccat agcgcgcttc gggtatgacg ctgatatagc ggaggtgaag gggagggatc
     9541 gcaccgggaa cagccatgac gcctattcca ttgatatgga atcagtcggc atcgacgaga
     9601 agacacgcac tgttttctta caggaaggac agacgcttga tactggtggg tataccaaag
     9661 gccataccgc gcagctgatg gcagaagcag cggttggctg tcagggtgtg ctcgtaaatt
     9721 taggaggaga catctatgca cgcggactcg acgcagaggg gaagcccttt gtctttcata
     9781 tcgataatcc gaaagactca agcatggatc tctcgttctt tgtaacgaac gcgggcattg
     9841 caacgagcgg gtcgtataac cgtacatgga tgctcgaagg aaaacctttc tttcatattc
     9901 ttgatgcgtc aggaaccaaa aatccagtca cggacatagt gtccgctacc gtcattgcac
     9961 cctcaggagc ggaagcagat gcctttgcta ccgtagcact cgtcgcaggg gtgcaggcgg
    10021 cagagtgcct cttggaagag gagggctacg aatactgctt tataaccacc aatggaagca
    10081 tcatcttctc gggtgcattc ccgctcgtag agaatgccga gtcctacctc tatgtctaat
    10141 aaagcctatt ttgccttcat agcatggttc caagaggcgc ttcttgcgct cggcatcggg
    10201 atacttatcg tacttcccgt tatgctcgcg taccttcctg agtatctgcc cgactggtcg
    10261 tacacgactc tattcactat ttcgttggtc gcggtatttc tcgtcatgat gatacgtccg
    10321 ctcgcggatc tatttccgaa cgtcccctgg cttcgcccgc tcgttatctt gcgcaaagga
    10381 ttcggcgtcc tctctgcctc cattattgtc ggcatcatgt tttcaaagtt catggtcgac
    10441 ggggtctcct atgtcatcga cttctttagt cctgaacgat ggtcgcttgc agaaggcgcc
    10501 atacttgctc ccttagctga tctttctgca ctccttcttc ttatcacctc gaataagttc
    10561 tccaagcgcg tgcttgctaa gaactggaag cgactccaga agctcgcgta tgtgtacttc
    10621 tatgccggtg cgttgtatga atacgtgttg ctcggacaag cgtttgcgct cgttgctatg
    10681 gtactcgtga ccatcgttgt tgttgctgcc tttgtgaaga atcggtgggc acctgctgct
    10741 tcactccagg cggtatgacc ataaaaagaa tgccagcaac tatagggacg ataactgacc
    10801 tttcgccaac ggcacgagag gtgacgctac accttcctga actcctcggg tttactcccg
    10861 gtgcgtttgt taatgtcttc atggatcatg acggaacacg gatgcgacgc gcctactcca
    10921 tctcttcaga cgagacggcc caagacatga ttgcgctctc tatccgtaag gggagtgtgg
    10981 gcggtatgag cgaacgcttt tgggatacgg gggtggctga ggtgccgctt cagatcatgg
    11041 gaccgctcgg ccttaatact gccgacaaga ttacgggcac tcgagtgttt ctgtttgcat
    11101 ttggtatcgg ggtatcagta attaaaggac tgcttccgca cttactgcgc cgacgtgata
    11161 tcacccacat aacggttatg acggggagcc gaaacgaaga agaggcgcta tataaagaat
    11221 tctttgaaga agcagcgcga cgcgaccctc gtctcaccgt gacgatggtg ctttcaaaac
    11281 cgcacgatac agcgtacccc tataaaggac atttacagga tgctatcggt ggaatcgatt
    11341 ttaccgatgc gtccgtctat atatgcggaa caaaagcagc ggcagaatca ctacagactg
    11401 ccataacgaa gaagggtcag cccgtgcatc aattccttat tgaagcgttt gattagtgtt
    11461 acttctcctg ccacacccgc ttatggctct ctatctcacg gatagagcgg ttacggtgaa
    11521 tcaactcatc attgcgtact gactcgaaga attcgtattc gttacgtacg ttacgaccat
    11581 agaagtcgag gaagtcagac acgactggta tctgtttaag actttcacct tgctcatcaa
    11641 ggaactgctg gtactgatga taggcatgag actcaaagag gtagttcaat tcaagcgcag
    11701 cgcgatgcga gaagatatac acgagataca cgacccagaa gtagaagaat gaaaaaagaa
    11761 gcgggaccaa ggtgtgacgg aagaatgcgt cgcgaccctt tttgcgcacc aggctcgaga
    11821 tgaccaccac gtgcatcgtc tcgttatcct gtgcaaaccg tccgaacgcc gccatgcggc
    11881 agagacgtat ggcgtgcttt tcgtttccgt agaacgcggt gagcatggtg taggcagcag
    11941 actcccagga ctggtagggg atacgtgcta tcacttcaac ggctttgaac ttgagatagg
    12001 agggcctctt tccatagaag gtgttgccgg cagtcacgag gagccaacca aggatacccg
    12061 ggataatgcc ggtgcggtag cgattcagtg gctgtgcgta ctcctcgcgc agcttcggat
    12121 cgtttaactc ttgcaccagt ttctcatgag cgacgtaatc atcgttcatt tggaaggaag
    12181 gagcagtaga ttccatatct gtcgattata tcg
//
LOCUS       DAORIN010000009        12156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_890_length_12156_cov_5.541608, whole genome shotgun sequence.
ACCESSION   DAORIN010000009 DAORIN010000000
VERSION     DAORIN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_890_length_12156_cov_5.541608"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK109_00735"
     CDS             complement(<1..307)
                     /locus_tag="PK109_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24102.1"
                     /translation="MALAYEGPKQPLSSHEEELAHLRAQVEAKERQLESMKQSRPRES
                     IVRERLRHHLETPAESVLAPEMHMSQREANEIALNLDPESDDETMEELRRIMEARGIH
                     "
     gene            complement(376..714)
                     /locus_tag="PK109_00740"
     CDS             complement(376..714)
                     /locus_tag="PK109_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRH24103.1"
                     /translation="MMNIIHWIVSAVAILIAAYLIPGVNVTLIGALVLAVVLALINVF
                     LKPILTLLTLPLNIVTLGLFSLVLNALLIMLAGAIVPGFSVDGFWAAFFFAIVVSLVN
                     ALFGGMGKKI"
     gene            complement(766..1668)
                     /gene="pyrB"
                     /locus_tag="PK109_00745"
     CDS             complement(766..1668)
                     /gene="pyrB"
                     /locus_tag="PK109_00745"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRH24104.1"
                     /translation="MKHVIDIRDFDREALEALFKKVDGMLEKPPVDALKGKIMAALFY
                     EPSTRTRLSFESAMNRLGGAVIGTENAGEFSSAAKGETLEDSIRVVAGYADVIVIRHP
                     EVGSAERASSASPVPVINAGDGAGQHPTQALLDAYTIQRETGGLDGKKVALVGDLKNG
                     RTARSLAYLLSKFTGVEIMLVSPKELAMGQDIKEYLSGKGMTLTESEDLAGALAWADV
                     VYQTRIQKERFASEEEYLKFKGRFVITRALADTMKDGAIIMHPLPRVDEIAPEVDDSP
                     HARYFEQARYGLYVRMALLHELLH"
     gene            complement(1665..2342)
                     /gene="pyrF"
                     /locus_tag="PK109_00750"
     CDS             complement(1665..2342)
                     /gene="pyrF"
                     /locus_tag="PK109_00750"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRH24105.1"
                     /translation="MNERIIISADELAEQELLALASSIGERVYAIKIHDAYDRLGPGI
                     VGELKDAGAHKVWMDAKIHDIPNTARARAKAFAENGIDIITVHASGGVEMMRAAKEGA
                     GSAEVYAVTVLTSLSEEAASTIYHQKVMEQVLALAHLAKEAEVDGVVCSAKEVAMLAA
                     DPALTGLKFVVPGVRSPGADTHDQKRVMTPREAINAGATHLVIGRQVTTAQDPASALS
                     AVESELL"
     gene            complement(2339..2926)
                     /locus_tag="PK109_00755"
     CDS             complement(2339..2926)
                     /locus_tag="PK109_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HRH24106.1"
                     /translation="MTTCILHGGYTREDNELNAAFYRDFAAAIPDGSTILYVYFATRV
                     GKESGDADRFVEHQRFVAAGAPEKNFTSVFAEKDSFVEQARAADAIFINGGVPENLMA
                     VLHLVPDIKGLFEGKIIAGSSSGAFALSKYYYSIDTTPGVYEGFGIAPVRVLCHYMSE
                     TDEYIGDEGIEALKAYPDTFELVVLKDCEHKVITV"
     gene            complement(2923..3552)
                     /locus_tag="PK109_00760"
     CDS             complement(2923..3552)
                     /locus_tag="PK109_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRH24107.1"
                     /translation="MKDELIALLKETGALITDSHLVGVSGRHMSVYVNKDALIPHTSA
                     TSRVGEMFAEQNKDLDIDVVVAPAMGAIILGQWTAYHLSKLKGKEILSVYTDKTGDDD
                     QVLKRGYDKMVAGKNVLCIEDTTTTGGSVKKVAEKVRDAGGMVVGVRVMINRDAKNIT
                     SESVGFPFDALATIDADSYAEEECPLCASGVPINTTVGHGKKFLEKKSS"
     gene            complement(3558..4631)
                     /locus_tag="PK109_00765"
     CDS             complement(3558..4631)
                     /locus_tag="PK109_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRH24108.1"
                     /translation="MLRTPFFDPNKTYAENWEQGPFGDFADGVVLEGSEPRFEFLGQK
                     ISYPLGIPAGPLLNGKFVDAALDKGWDVPVHKTVRSAVHPCNDWPNVLALKDFPGPLS
                     IERAKVPLVVTNEYSEPLSITNSFGNPSYDPEVWKADLRKSLAHAKPGQLVMCSFEGT
                     KREGMSTEEYIADWADGMKAIADTGVKIVEANFSCPNEGAATLLCFDVPKVKTVVTAI
                     KEKIGTVPLVIKIAYFEEAALRELVEETGNLVDAYSAINTIPAPVVNEDGSQALPGAH
                     RVKSGICGHAVKWAGVDMVKRLKALREELGMSYGIIGSGGVTVPADFKEYRDAGADLV
                     MSATGAMWNGYLAKEIKEAYPDA"
     gene            complement(4635..5669)
                     /locus_tag="PK109_00770"
     CDS             complement(4635..5669)
                     /locus_tag="PK109_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HRH24109.1"
                     /translation="MTTRFPGLIDAHVHLREPGATHKEDFLTGSRAAAKGGVTFMIDM
                     PNNMTPTISIERLDEKVALSNKAIIDIGFHFGTNGQNLEELPKAAARSEVYGLKIYCN
                     HTTGEMLIEDQSVLDAIFQAWPVGKPILVHAEGEQLKQSIARAKKFGQRLHVCHITQA
                     TEVEDVRVAKAEGLTITAGVCPHHLALTAEARERMKGYAVMKPPLGTDDDQAALWAGL
                     ADGTIDIVETDHAPHSREEKEKDPPMFGVTGLETSFGIIYKGVKEGKITQERLIDAMH
                     TMPKKIFNTPDQGDTYIEVDTDATWVVGAEGYETKAASSPFEGWELPGKVMTVVLRGT
                     TIVEEGKIII"
     gene            complement(5687..6346)
                     /locus_tag="PK109_00775"
     CDS             complement(5687..6346)
                     /locus_tag="PK109_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24110.1"
                     /translation="MTKFGWLVVLGVLLVLGGIAAIIYFIPKTAPAPEPVEVGTENLE
                     GQSIYASGEYGFTLRYPSKATVEEVFTPSYIIGTTWRANAIATGTPLLAVINYTTESD
                     HSFPRNYTAMVRVGVSVDEVENCEKVTPDAGETALPDREINGTTWKAFSFGDAAMMKY
                     VKGVSYRVEHNGTCYALEKIATGSSYRDDPESADDISQDTLDAEYAALDAIIETFSFS
                     R"
     gene            complement(6378..8042)
                     /locus_tag="PK109_00780"
     CDS             complement(6378..8042)
                     /locus_tag="PK109_00780"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRH24111.1"
                     /translation="MTQNEALNILKTGANAFLTGEPGSGKTHTVNAYVEWLRSHDIEP
                     AITASTGIAATHVGGMTIHAWSGIGIREYLSASDLDAISSKEHIVRRLNKTAVLIIDE
                     VSMLSGEVLSMVDQVLREVRHSERAFGGVQVVLVGDFFQLPPVSKASREASFAFQSDA
                     WRRLNPIPLYLEEQHRQEDAKFGGILASIRSGTFDHTDMSHISSRQTEPEDAGDIPRL
                     YTHNADVDRINAEKLASIPGSGKGFLMDTSGAPTLIESLKRGCLSPERLELKVGAVVM
                     FTKNNPLAGFANGTLGVVAGFENGTGYPVVELRDGRRIAVAPQEWVVEENGKVRAKLT
                     QVPLRLAWAITIHKSQGASMDAAMIDLSRAFEYGQGYVALSRLRTLEGLYLLGWNENA
                     LAIHPAVRSVDERFREASGEAAAVFNSLEETGERRVLEENFLTASGGSLEVIDTDAPR
                     GEKPTTYDETYMLLKEGKTLEEVVKARELTFGTIVSHLEKLSEAGKLSTEQVRALASK
                     RLIDGLGAISEAAGNKSFKALTPVFKKLKGKYTFDEIRLARLILAE"
     gene            complement(8069..8494)
                     /locus_tag="PK109_00785"
     CDS             complement(8069..8494)
                     /locus_tag="PK109_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRH24112.1"
                     /translation="MILQVGVKALLRNTEGRVLLLKRSSRYGVVEGSWDIPGGRIDVG
                     TSLMENLSREIHEETGLTAVSHPKLIAAQDLLPKENMPNHVVRLTYVAEVSGEPVLDG
                     TEHTEYRWVSFDELATLPNLDAYLAKLVSEGVITADSWN"
     gene            complement(8491..8850)
                     /locus_tag="PK109_00790"
     CDS             complement(8491..8850)
                     /locus_tag="PK109_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24113.1"
                     /translation="MRFGSTTDFLLRLGVAFSFLFPPVNAFLDPYAWVGYFPAFIPDA
                     MPEMVLLHVFGLIEVIIGLWLLSGRKIFIPSVAATALLALIVLFNLGDFQVLFRDVSI
                     AFAALALAIAHRPKSAV"
     gene            complement(8853..9335)
                     /locus_tag="PK109_00795"
     CDS             complement(8853..9335)
                     /locus_tag="PK109_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24114.1"
                     /translation="MNAKTIWIVVGIIVVLGLGAWWLSTMTPAAPVTTEDTAEVAGAD
                     NQFVDDSKDGAAEAVTVSYTNDGFSPSTVTIKKGQSVTFVNKGTQEMWIASDEHPTHT
                     GYDGSNKDAHCAQGYTGEKPLDQCGVSGSYTFTFTKAGTWGYHNHREDDDHGTVIVTE
                     "
     gene            complement(9411..>12156)
                     /gene="carB"
                     /locus_tag="PK109_00800"
     CDS             complement(9411..>12156)
                     /gene="carB"
                     /locus_tag="PK109_00800"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HRH24115.1"
                     /translation="KDALRAAEELGYPIMARSGFALGGEGSGLVRTAAALSEKLGEVF
                     HTNAQVLIEEYLEGWKEVEYEVVRDRMDNCVTVCNMENIDPMGVHTGESMVVAPSQTL
                     TNAEYHMLREVAITTIRHLGIVGECNIQYALHPKTGDYRVIEVNARLSRSSALASKAT
                     GYPLAFVAAKLALGHSLSSLPNSVTKVTNACFEPALDYLVIKMPRWDLNKFENSNKQI
                     GTEMKSVGEIMAIGRSFEEVFQKGIRMLSIGADGFTAQRNANFRIEDYLKEIKTPTTT
                     RVFALAEALRAGVSVQKIHAMSGITPWFLEKMQNIVAMERSLKRSPLNKASLIKAKKA
                     GFSDKQLATIKKTTEAKIRAQRLRLGVRPVAKQIDTLAGEFPAQTNYLYLTYHGTEND
                     VTPGKKSIMVLGSGPYCIGSSVEFDWSSVQALNTLRAEGRETVMVNYNPETVSTDYDM
                     SDRLYFEELSLERVLDIHDFENPSGTIVSTGGQIPNNLALPLSDNGVKVLGTNPKDIN
                     RAEDRHVFSALLDKLAVDQPAWVELSSLKQATKAAAELGYPVLIRPSFVLSGAAMSVA
                     FDETSLVKFLKRATAVSNDHPVVISKFIENAREIEIDGVAQKGELVLYAITEHVENAG
                     THSGDATVVTPPQRTYLETIRRAKDITKKILKELRITGPFNIQFLAKDNNIQVIECNV
                     RASRSFPFVSKTTGHNFIEIATKALMGQDVRGNYQTLELDYVAVKSPQFSHSRIKGAD
                     PLLYVEMASTGEVACFGNSYYEALLKSMLSAGFRLPKKNILVSLGGDENKVKLLPALT
                     ELAGLGFSFFASEGTTEFLRENGIECSKVHKITAKKEPNILSLIENGNIDLIINIPTR
                     PLARENEDGFVIRRKAVDLNIPLITNRQLAEAFITALAETGGVTLPVKPWSAYSPDKH
                     "
BASE COUNT         2794 a         3345 c         2956 g         3061 t
ORIGIN      
        1 tatgaatgcc acgtgcttcc ataatgcgac gaagctcctc catcgtctca tcatccgatt
       61 ccggatcaag attgagcgca atctcgttcg cctctctctg actcatatgc atctcaggcg
      121 caagcacgct ctcagcaggc gtctctaagt ggtggcgcaa tcgctcacgg acgatgcttt
      181 cgcgaggacg cgactgcttc atactttcaa gctgccgctc tttcgcttct acctgtgcgc
      241 gcaggtgggc aagctcttcc tcatgggaag agagcggttg tttaggtcct tcataggcca
      301 acgccattga cctctagtat acgacacgcg cgcgccataa cggcgcgcgc gtggagaaca
      361 aaacaggaat gaagcttaga tcttctttcc catgccgcca aagagtgcgt tcacaagcga
      421 cacgacgata gcgaagaaga atgctgccca gaatccgtcc accgagaagc ctggcacaat
      481 ggctccggca agcatgatca agagagcgtt caagacaagc gagaagagcc caagcgtcac
      541 gatgttcaat gggagcgtga ggagggtgag aatcggtttc aagaatacgt tgatgagagc
      601 gagcacaacc gcaagcacca atgcgccaat aagggtgacg ttcacacccg gaataagata
      661 cgccgcgata aggatggcaa cagcggaaac tatccagtga atgatgttca tgatcgttgt
      721 tatgaagggc taatggcgct aaggatacgg atattcgatg aacgcttaat gaagaagttc
      781 atgaagcagc gccatacgta cgtataaccc gtaacgagcc tgttcgaaat agcgggcatg
      841 gggagagtca tctacctcag gcgctatctc atccacacgc ggcaagggat gcatgatgat
      901 ggcgccgtcc ttcatagtgt cggcgagcgc tctcgttatc acgaagcgtc ctttgaactt
      961 caagtactcc tcttctgaag cgaatcgctc tttctggatg cgggtctggt acaccacgtc
     1021 tgcccaggca agcgcgcccg caagatcttc actttcggtg agcgtcattc cctttccgga
     1081 gaggtattcc ttaatgtcct gccccattgc aagttcttta ggagaaacca gcatgatctc
     1141 aacgcccgtg aacttagaga gaaggtaggc aagggagcgc gctgtgcgtc cattcttaag
     1201 gtcgcccacg agcgctacct tctttccatc aagtcctccg gtctcgcgct gtatggtgta
     1261 tgcatcgaga agcgcctgtg tagggtgctg ccctgcacca tctccagcgt taatgaccgg
     1321 taccggagac gcacttgatg cgcgctctgc agaccctact tctgggtgac gaataacaat
     1381 gacatcagcg tatcctgcca ctacccgaat actatcttca agcgtttctc cttttgctgc
     1441 agaagagaac tcacctgcgt tctccgttcc aatgactgca ccgccgagtc gattcattgc
     1501 tgactcaaac gaaaggcggg tgcgggtcga tggttcatag aagagtgccg ccatgatctt
     1561 tcctttcagt gcgtctactg gaggcttctc gagcatcccg tccaccttct taaaaagcgc
     1621 ctcaagcgcc tccctatcaa aatctcgtat atcgatgacg tgcttcatag tagttctgat
     1681 tctaccgcgc taagggcgct agccgggtcc tgtgccgtgg ttacctgtcg cccgataacc
     1741 aagtgcgttg cacctgcgtt tattgcctct cgtggagtca tcacacgttt ctgatcgtgc
     1801 gtgtcagcgc ctggagaacg tactcctgga accacaaact taaggcctgt aagggctggg
     1861 tctgccgcaa gcatcgccac ctcttttgct gaacacacca ctccatccac ttcagcttct
     1921 ttcgcaagat gcgcaagcgc gagcacctgc tccattactt tctggtgata gatggtgctg
     1981 gctgcttctt cggagagtga ggtgagcacg gtcactgcat acacttcagc acttcctgca
     2041 ccttccttgg ccgcacgcat catctcaacg ccgcctgacg catgtacggt aatgatatcg
     2101 atgccgttct ccgcaaacgc tttcgcacgg gcacgagcgg tattcgggat gtcgtgaatc
     2161 tttgcgtcca tccatacctt atgcgcgcca gcatccttaa gttctccaac gataccggga
     2221 ccaaggcgat cataggcgtc atggattttg attgcgtata cgcgttcacc tatcgaactc
     2281 gcgagcgcta ggagctcttg ctcagcaagc tcgtctgcag aaattattat gcgttcgttc
     2341 atactgttat tactttatgt tcacaatctt taagtaccac caattcaaaa gtatctggat
     2401 acgctttcaa ggcttcgatg ccttcgtctc caatatattc atccgtttca ctcatgtagt
     2461 ggcatagcac gcgtaccgga gcaataccaa aaccttcata gacaccaggg gtcgtatcaa
     2521 tagaataata gtactttgaa agtgcgaaag ctcctgagga agatcctgcg ataatctttc
     2581 cttcaaataa ccctttgatg tctggtacca gatgaagaac ggccatgagg ttctctggta
     2641 cccctccatt aataaaaata gcatcagcgg ctcgcgcttg ttcgacgaag gaatccttct
     2701 cggcaaagac tgaggtgaag ttcttttcag gagcgcctgc ggcaacaaac ctttgatgtt
     2761 ctacaaagcg atccgcatct cctgactcct ttcctactct cgttgcaaaa tagacgtaaa
     2821 gaatcgtact gccgtcagga atagctgctg cgaaatcacg atagaacgca gcgttaagct
     2881 cgttatcttc ccgtgtgtat ccgccatgca gaatgcaggt ggtcatgagc ttttcttctc
     2941 gagaaacttc tttccgtgcc ctaccgtcgt attaatagga actccggaag cacacagagg
     3001 acactcttct tcggcatagg agtcggcgtc gatggtagcg agcgcatcaa acgggaatcc
     3061 tactgactcc gaggttatat ttttcgcatc gcgattaatc atcacccgca caccaaccac
     3121 cattcctccc gcatcgcgta ctttctcggc caccttcttc actgaacctc cggtagtagt
     3181 tgtgtcttca atgcagagca cgttcttccc tgcgaccatc ttgtcataac cacgcttcaa
     3241 cacctgatca tcatctcctg ttttatccgt atataccgaa agaatctctt tccccttcaa
     3301 ctttgaaaga tggtacgcgg tccactgacc caaaataatg gcgcccatgg cgggcgctac
     3361 gacaacatca atatccaaat ccttattctg ctcagcaaac atttcaccca cgcgggacgt
     3421 tgcactggta tgcggaatga gcgcatcctt gtttacatac acactcatgt gacggccaga
     3481 aacaccaacc aggtggctgt cagtaataag agcgcccgtc tctttaagaa gcgcaatgag
     3541 ttcgtctttc atatagctta tgcgtcagga tacgcctcct taatctcctt ggcgagatac
     3601 ccgttccaca tcgcgcccgt agcgctcatc acgaggtctg cgcctgcatc tcgatattct
     3661 ttaaagtcag cagggacggt aacgccgcct gagccgatga tgccgtagct cataccaagt
     3721 tcttcacgga gcgctttgag gcgcttcacc atatcaacgc ctgcccactt cacggcgtgg
     3781 ccacaaatac cgcttttgac gcggtgcgca cctggaagcg cctgacttcc gtcttcgttc
     3841 acgacagggg ctggaatcgt gttaatcgca gaatacgcat ccacaagatt tcccgtttct
     3901 tctacaagct cacgaagtgc tgcttcttca aaatacgcaa tctttatcac gagcggcacg
     3961 gtccctatct tctctttgat agcggtcacc accgtcttta cctttggtac atcaaagcaa
     4021 agtaaagttg ctgcaccttc gttcgggcaa ctgaagtttg cctccactat ctttacgccc
     4081 gtgtcagcaa tagccttcat gccatctgcc cagtcagcaa tatattcctc agtgctcatt
     4141 ccttcgcgct tcgtgccttc aaacgagcac atcacgagtt gtcccggctt tgcatgcgca
     4201 agtgactttc gtagatccgc cttccatacc tcagggtcat aggacgggtt accaaatgag
     4261 ttggtgatgg agagcggttc tgagtattcg ttcgtgacca cgagcggtac tttcgcgcgt
     4321 tcaatagaga gaggtcctgg gaaatcctta agcgccaaga cgtttggcca gtcattacat
     4381 ggatgtaccg cgcttcgtac cgtcttatgc accggcacat cccatccttt atccaacgct
     4441 gcatctacaa acttcccatt caagagcggt ccagcaggaa taccgagcgg ataggaaatc
     4501 ttctgaccga ggaactcgaa ccttggttcg ctcccttcaa gcacgacgcc atcagcaaag
     4561 tctccgaacg gtccctgttc ccagttctct gcgtaggtct tgttcggatc gaagaatggc
     4621 gtgcgtagca taggttagat aattattttt ccctcttcta ctattgttgt accgcggagc
     4681 accaccgtca tgacttttcc aggaagttcc catccttcaa acggactcga tgctgccttt
     4741 gtttcgtatc cctcagcacc tactacccac gtggcgtccg tatcaacttc gatgtaggtg
     4801 tctccttgat caggcgtatt aaatatcttc tttggcatcg tatgcatcgc atcgatgagt
     4861 ctctcctggg taatctttcc ttccttcact cccttataga tgatgccaaa cgaggtctca
     4921 agaccggtga ctccgaacat gggaggatcc ttttctttct cttcgcgtga gtgcggtgcg
     4981 tggtccgtct caacgatatc aatagtgccg tcggcaagcc ctgcccaaag cgccgcttga
     5041 tcgtcatcag ttcccaaagg aggcttcatc actgcatacc ctttcatgcg ttcgcgcgct
     5101 tctgcggtga gcgcgaggtg atgcggacat acgcctgccg taatagtgag tccttccgct
     5161 tttgcgacgc gtacatcttc tacttctgtt gcctgcgtga tgtggcagac atggagacgc
     5221 tgcccaaact tcttagcgcg cgcaatgctc tgtttcaatt gctcaccttc tgcatgcacg
     5281 agaattggct ttcctaccgg ccacgcctgg aatatagcat cgaggacact ttggtcctcg
     5341 atgagcattt ctcccgtggt gtgattgcag taaatcttga ggccatagac ttccgaacgt
     5401 gcggcggcct taggaagttc ctcgagattc tgaccgttcg taccaaaatg gaatccaata
     5461 tcaataatcg ctttatttga gagcgctacc ttttcatcaa gacgctcgat ggagatggtt
     5521 ggcgtcatgt tatttggcat atctatcatg aacgtaacac ccccttttgc tgccgcacgt
     5581 gaacccgtca agaaatcctc cttatgggta gctcccggtt cgcgtaggtg cacatgagcg
     5641 tcgatgaggc ccgggaagcg tgtagtcatg gccacagggt agcgtattac cgcgagaacg
     5701 agaaggtctc tatgatggca tccagggccg cgtactcggc gtcgagtgtg tcctgggaaa
     5761 tatcgtctgc gctctccgga tcgtccctat agcttgaacc ggtggcgatc ttttcaagcg
     5821 cgtagcaggt gccattatgc tccacgcgat aacttacgcc tttcacgtac ttcatcatgg
     5881 cagcgtctcc aaatgagaat gctttccatg tcgttccatt tatttctctg tctgggagcg
     5941 ccgtttctcc agcgtctggt gttacctttt cgcagttctc tacctcatcc actgatactc
     6001 caacgcgcac cattgcggtg tagtttcgag ggaaggagtg atcactctcg gtggtgtagt
     6061 taatgaccgc aagaagtgga gtgccagtag ctatggcatt tgcacgccac gtggttccaa
     6121 tgatgtagga aggggtaaag acctcttcaa cagtcgcctt tgaggggtag cgcagcgtaa
     6181 acccatactc accacttgcg tagatggatt gtccttcaag attctcagta cctacctcca
     6241 ctggttctgg cgctggtgcg gtcttcggga tgaaatagat gatagccgct atgcctccca
     6301 gaacgaggag cactccgagg acgaccagcc acccgaattt cgtcatatcc ctagtatacc
     6361 aaaccctcag taatgagtta ctccgctaaa atgaggcgcg ctaatcgaat ctcatcaaat
     6421 gtgtactttc cttttagttt cttaaacacg ggcgtgagcg ccttaaagct tttattcccc
     6481 gcagcttctg atatggcgcc gagtccgtcg atgagacgct ttgaagcaag agcacgcacc
     6541 tgctcggtag aaagttttcc ggcttcggaa agcttctcga ggtgactcac aatggtgcca
     6601 aaagtaagtt cacgtgcttt caccacttcc tctaacgttt ttccttcctt gaggagcata
     6661 tacgtttcgt catacgtcgt cggtttctca ccacgcggcg catccgtatc aatgacctca
     6721 agcgaaccac ccgacgccgt caagaagttc tcttcaagca cgcggcgctc tccggtctct
     6781 tcaagactat tgaatactgc tgccgcttct cctgaggctt cgcggaagcg ctcatccaca
     6841 gagcgcactg caggatgaat ggcgagcgca ttctcgttcc agcctaggag atagagtcct
     6901 tcaagcgtac gtaggcgcga gagtgctacg tacccttggc cgtattcaaa ggcccgcgaa
     6961 agatcgatca tcgccgcgtc catacttgcc ccttgactct tatgtatcgt tattgcccac
     7021 gcgagtcgaa gcgggacctg agtaagtttt gcccgcacct taccgttctc ctccaccacc
     7081 cattcctggg gcgctacggc aatccttctg ccatcacgaa gctccaccac gggatacccg
     7141 gtcccgtttt caaatcctgc gacaacccct aaggtgccgt ttgcaaaccc tgccagcgga
     7201 ttgttcttag tgaacatcac caccgccccc acctttagtt caagacgttc aggagaaagg
     7261 catccgcgct tcaaggattc aataagggta ggtgcacccg aggtgtccat gagaaaacct
     7321 ttgccacttc ccggaatact tgcgagcttc tctgcattaa tgcggtccac gtccgcgttg
     7381 tgcgtgtaca gacgcggaat gtcacctgcg tcctctggtt ccgtctgtcg cgaagagata
     7441 tgcgacatgt cggtatgatc gaaggtgccg cttctaatac ttgcgagtat gccaccgaac
     7501 ttcgcgtctt cttggcgatg ctgttcttcg agatagagcg ggatgggatt caagcgacgc
     7561 caggcgtctg actgaaacgc aaacgacgct tcacgagacg cctttgaaac cggcggcaac
     7621 tggaagaagt cgccgacgag caccacttgc acaccaccga acgcgcgctc gctatgacgc
     7681 acttcacgca gcacctgatc taccatcgac agcacttctc cggagagcat cgacacctcg
     7741 tcaatgataa ggaccgccgt cttattcaag cgacgtacga tgtgttcttt tgaagagatg
     7801 gcatcgaggt cagaggcgga aagatattca cgtatgccaa taccgctcca cgcatgaata
     7861 gtcatgccgc ccacatgggt cgctgcaata ccggtagaag cagtaatagc aggttctata
     7921 tcgtgcgagc gcagccactc aacgtatgcg ttcaccgtat gcgtcttacc gcttcctggc
     7981 tcacctgtga gaaaggcgtt agcaccagtc ttcaatatat tcagcgcctc attctgagtc
     8041 atgaagcagt cattgtaccg gacctatctc aattccagga atctgcggtt atcacccctt
     8101 ctgacacaag tttagccaga tacgcatcaa gattcgggag cgtagcgagt tcatcaaaag
     8161 aaacccaacg gtattcagta tgttccgtac catcgagtac cggctctccg cttacctcag
     8221 caacatacgt aagccgtacc acatgattgg gcatgttttc ttttggcaac aaatcttgtg
     8281 cggcgatgag ttttggatgc gatactgcgg tgagtcccgt ttcttcgtgt atctcgcgcg
     8341 aaagattctc cataagagaa gtaccaacat ctattcgccc gccaggaatg tcccacgaac
     8401 cttcaacaac gccatagcgt gatgaacgct tcagaagaag caccctaccc tcggtattac
     8461 gtaagagcgc ctttacccca acctgaagga tcatactgcc gacttcggac gatgcgctat
     8521 cgcaagggcg agcgctgcaa acgcaataga gacgtcgcgg aacagcacct ggaagtcacc
     8581 gaggttgaag agcacgatga gagcaaggag tgcggtagct gcaacggacg gaatgaatat
     8641 cttcctccct gaaagtagcc aaagaccgat gatgacctca atgagaccga atacatgaag
     8701 gagtaccatc tctggcattg catccggaat gaacgcaggg aaatacccga cccatgcata
     8761 gggatcaaga aaagcgttca ccggcgggaa caagaatgag aaggcaacgc ctaagcgaag
     8821 caggaagtcc gttgtgctac cgaagcgcat agttattcag ttacgataac cgtaccatga
     8881 tcatcgtctt cacgatgatt gtgatagccc caggtaccag ctttggtgaa cgtgaaggtg
     8941 tagctgccgc tcaccccaca ctgatcaagt ggtttctctc ccgtgtatcc ttgcgcgcag
     9001 tgcgcatctt tgtttgagcc gtcatagccg gtatgcgtgg ggtgctcgtc tgaagcaatc
     9061 cacatttctt gcgttccttt atttacgaac gtaaccgact gtcctttctt aatcgtcacg
     9121 gtgcttggtg agaatccgtc gttagtgtat gagacggtca ccgcttcagc agcaccatcc
     9181 tttgagtcat caacaaactg attgtccgcg cctgcaactt cagcagtatc ttctgtggtc
     9241 acgggcgctg ctggagtcat ggtagagagc caccacgccc cgagcccaag gactacgata
     9301 atgccaacaa caatccatat cgtctttgca ttcatacgtg cgtacgttag ccagtaaagt
     9361 cttcaccagt ataccaatat cgtagcgtcc atgaacgctt catgagggcg ctagtgctta
     9421 tcgggagagt acgcgctcca gggctttacg gggagtgtca cacctcctgt ttcagcaagc
     9481 gcggtgatga atgcctctgc aagctgacgg ttggtgatga gaggaatatt caagtccacc
     9541 gctttacgac gaatgacgaa tccgtcttcg ttctcacgtg ccagagggcg cgtgggaatg
     9601 ttgatgatga gatcgatgtt cccgttttcg ataagggaaa ggatgttagg ttctttcttc
     9661 gccgttatct tatgcacctt actgcactca ataccgttct ctcgtagaaa ttcagtggtg
     9721 ccttcagacg caaagaacga gaacccgaga ccagcaagtt cggtcaacgc cggtaggagt
     9781 tttactttat tctcatcccc accaagcgac acaagaatgt ttttctttgg gaggcggaat
     9841 ccggcagaga gcatcgattt caataacgcc tcataatacg agtttccgaa gcatgcaact
     9901 tctccggttg aagccatttc gacgtagaga agcgggtcgg cacccttgat acgggaatga
     9961 gagaactgtg gagactttac ggcaacatag tcgagctcca aagtctgata gtttcctctt
    10021 acgtcctgac ccatgagtgc cttcgtggct atctcaataa agttatggcc ggtggtctta
    10081 gagacaaacg ggaaggaacg ggacgcacgc acgttacatt cgatgacctg gatgttgttg
    10141 tccttggcaa ggaactgaat gttaaatggc cccgtgatgc ggagttcttt caatatcttc
    10201 ttggtgatgt ctttggcgcg gcgaatagtt tcaagatagg tacgctgggg tggcgtcact
    10261 acggtagcgt ctcctgagtg ggtgccggcg ttctccacat gctcggtaat agcgtagagc
    10321 acgagttccc ctttctgcgc cacgccatca atctctatct cgcgcgcatt ctcaatgaac
    10381 tttgagataa ccaccggatg gtcgtttgag actgccgttg ccctctttaa gaacttcacc
    10441 aaggacgtct catcaaaggc taccgacatc gcagctcccg aaagcacgaa ggatggacgt
    10501 atgagtaccg ggtacccgag ttcagcagca gccttcgttg cctgctttaa acttgaaagt
    10561 tctacccatg ctggttgatc taccgcaagc ttatccaaga gtgccgagaa gacgtggcgg
    10621 tcttcagcgc gatttatgtc ttttggattg gtaccgagca cctttacccc attatctgaa
    10681 agtgggagcg cgaggttatt aggaatctga ccacccgtag aaacaatagt gcccgaagga
    10741 ttctcgaagt catgaatatc gagcacgcgt tcaagcgaaa gttcctcgaa atagagacgg
    10801 tccgacatat cgtagtccgt ggagaccgtc tcagggttgt agttcaccat taccgtttcc
    10861 ctaccttcgg ctcgaagcgt gttcagcgcc tgtacactcg accaatcgaa ctctactgat
    10921 gaaccgatac agtacggacc tgagccaagt accatgatgc ttttctttcc aggagtcaca
    10981 tcgttttccg taccgtggta ggtgagatag aggtagttgg tttgtgcagg gaattctccc
    11041 gcgagcgtgt ctatctgctt tgcaaccggg cgcactccaa ggcgcaggcg ctgcgcacgt
    11101 attttcgctt cagtagtttt ctttatggtc gcgagctgct tatcagagaa ccctgctttc
    11161 ttcgccttta tgagactcgc tttgttgagc ggtgagcgct tgaggcttcg ctccatcgcg
    11221 acgatgttct gcatcttctc aaggaaccac ggcgtaatgc cggacatcgc atgaatcttc
    11281 tgaaccgaca ccccagcgcg cagtgcttcc gcgagcgcaa agactcgcgt cgtggtcggg
    11341 gtcttaatct ctttgaggta gtcctcgatt cggaagtttg cgttacgttg cgcggtgaat
    11401 ccgtcagcac caatggacaa catgcgaatc cctttctgga agacttcttc aaaggagcgc
    11461 cctatggcca tgatttctcc cactgatttc atctccgttc ctatctgttt gttggagttc
    11521 tcaaacttat tcaaatccca ccgtggcatc ttaatgacga gatagtccaa ggctggctca
    11581 aagcaggcgt tagtgacttt cgtcacagag ttcgggagcg aggagagcga atgtccgagt
    11641 gcgagctttg ctgcgacaaa cgcaagcggg tagccggttg cttttgaagc aagggctgat
    11701 gagcgtgaga ggcgtgcgtt cacttcaatg acgcgatagt caccggtctt cgggtggagt
    11761 gcgtactgaa tattgcactc acctacgatg ccgaggtgac ggatagtcgt aatcgcaact
    11821 tcgcgaagca tgtgatactc cgcatttgtg agtgtctgcg atggcgccac caccatactc
    11881 tctccggtgt gtacccccat tgggtcgata ttctccatgt tgcagaccgt cacgcagtta
    11941 tccatgcggt cacgcactac ttcgtattca acttccttcc acccttcaag gtattcctca
    12001 atgagcacct gtgcgttcgt atgaaatact tccccgagct tttctgaaag cgctgcagcg
    12061 gtacgcacga gtcccgagcc ttcgccaccg agcgcaaacc cacttcgcgc catgatgggg
    12121 tacccgagtt cttctgctgc cctaagcgca tctttc
//
LOCUS       DAORIN010000010        10386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1103_length_10386_cov_5.582809, whole genome shotgun sequence.
ACCESSION   DAORIN010000010 DAORIN010000000
VERSION     DAORIN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10386
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1103_length_10386_cov_5.582809"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1341)
                     /locus_tag="PK109_00805"
     CDS             complement(265..1341)
                     /locus_tag="PK109_00805"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRH24116.1"
                     /translation="MPGKATKSAISTKSRTAVKPSFDSTTLVKKLLGEVSERAREVLT
                     LRFGLGTKSERETLEAIGERWGITRERVRQIEAAAIEAVRASKGFKDAKPAFDELCAY
                     VESQGAILPEDHLLSGLASDEKAKNRFRFLLVVGSAFFRERETDEFYARWHVNHATAK
                     KVHAALSGLYSSLSDTEVLSESEVFERFLTELKDVDVAYRNEEILKRWLALAKNIDSN
                     PLAEWGRSDAPAIRTKGIRDYAYLVIKREGTPMHFRDVANAIASLFAKKAHVATTHNE
                     LMKDERFVLVGRGLYALAEWGYKPGVVRDVIREILRKFGPLSRERVLEEVKKVRYVKD
                     NTILVNLNDPKHFKRSKSGTYALA"
     gene            complement(1393..3777)
                     /gene="leuS"
                     /locus_tag="PK109_00810"
     CDS             complement(1393..3777)
                     /gene="leuS"
                     /locus_tag="PK109_00810"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRH24117.1"
                     /translation="MGKERFEHKPIEKAAQDKWDAAKLYETDLQDTSKEPYYLLFEFP
                     YPSGDLHIGHWYAYAVTDIYARLLRMKGKNVLFPIGFDAFGLPAENAAMKQGANPAEW
                     TLKNIEKMRAQIRSIGASLDWSKEVVTCSPEYYRWTQWLFAKLYEHGLAYRAEAAVKW
                     CPKDATVLANEQVIDGKCERCDTEVVEKRLTQWFMKIKDYADRLLSDLDELPWREDIK
                     EAQRAWIGRSQGARLWFPLQGREEKIEVFTTRPDTVFGATYVVLAPEHPLVDTLLTSV
                     GNEKEIRAYKDQTARKTERERSENKEKSGVRLEGVMAVNPATQKEIPIYVADYVLASY
                     GTGAVMAVPAHDERDHEFAKKYELPIQQVVEGEGECYTGDGKLINSGKFSGKDNTKAM
                     PEIVKFVGGEHTTNYRIRDWLISRQRYWGCPIPIVYDPEGRAHTVPAEHMPWLLPEDV
                     DFSPTGKAPLASSKELHERVERIFGKGWTPEYDTLDTFVDSSWYFLRYLAPNSTTEFS
                     DNELMKTWLPVKRYSGGSEHTTMHLLYARFFQKALYDLSLVPVSEPFQERYNRGLIMG
                     PDGQKMSKRWGNVVNPDEMLEKYGADAVRMYLAFVGPYNEPGHYPWKLDGVEAMRRFL
                     DRIYTLSAKTTATEVSIELQRAFATASAKIEGDAERFKFNTALSALMVLVKELETLEN
                     VPQDAWEEFLMLLAPFAPHLAEHLWGKLGKEGSVHQSSWPTADESLLVRDSVAVIVQV
                     NGKRRGEVDLAPDASEAAANEAALALPTVVALLEGKPPSRVIYVPGRIINIVIG"
     gene            complement(3782..4732)
                     /locus_tag="PK109_00815"
     CDS             complement(3782..4732)
                     /locus_tag="PK109_00815"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HRH24118.1"
                     /translation="MEPITKTTPKDFFLWLGAIVALYGSVTGFLTLVFTYINYAFPDP
                     LAYSGDPYGSGSHVAMATLIVLVPLTLVLFRIIRSTIVTEPGKAQIWVRRWALVLTLF
                     VAGAAVAIDLITALTTFLGGEISERFILKVAVVLLVALGIFLHFLADLKGYWVAHSKK
                     GNLVGVGVAVLVLATIIAGFFIIGTPSELRTLRYDAQKVSDLQNIQYQIIYYWQTKGE
                     LPATLAEVADPLSGSVIPVDPETGASYSYSVAGPLTFSLCSTFGAETPDTAGQGSYPG
                     RDIAYTSWGPQPDENWKHPAGEHCYERTIDPERYAPLKAQ"
     gene            complement(4784..5089)
                     /locus_tag="PK109_00820"
     CDS             complement(4784..5089)
                     /locus_tag="PK109_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24119.1"
                     /translation="MTRNPFLNALATVTYIAVVAAFLSFVPKFIPEPVTPIAPILGML
                     SLFVFSAAVTGYLMLGVPLQLLLEGQKKEGIALFAKTLVAFAGIAAVLSGVVLSLSL"
     gene            complement(5147..5524)
                     /locus_tag="PK109_00825"
     CDS             complement(5147..5524)
                     /locus_tag="PK109_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24120.1"
                     /translation="MSEKNLNSPLTPTQLRSLEQERKGLEEKVLRIKVLSPSAYNLMV
                     ELTDPSLSKEDFNRKIEEFNRPREKYRSTTNAQAISVDVADLLALGATFIKQMPESRQ
                     LYVRMWKVGDNQTVVFVDGHAGA"
     gene            complement(5866..6213)
                     /locus_tag="PK109_00830"
     CDS             complement(5866..6213)
                     /locus_tag="PK109_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24121.1"
                     /translation="MDLTAQNLEVVWITIIVLSCLSCWTTQEILFRRIKKLNYEKWVE
                     IGEPGFISALSPNLSKFKKAFKTLRYFTKGDTVLDSDPKTLKLKKLVKTVYLAVLIVV
                     VAFFVAVFIINPE"
     gene            complement(6255..7352)
                     /gene="ychF"
                     /locus_tag="PK109_00835"
     CDS             complement(6255..7352)
                     /gene="ychF"
                     /locus_tag="PK109_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006909031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRH24122.1"
                     /translation="MLKIGIVGLPNVGKSTLFNALTKAAVPAENYPFCTIDPSVGVVG
                     VPDERLQKLAAFSGSEKTIPAAIEFVDIAGLVKGASEGEGLGNQFLSNIRETDAILEM
                     VRFFEDPDVTHVHGEVDPVRDIEVINLELALADRDAVQKRREKLTRDIRAGMKEAVAE
                     DEALKKVEEALNAGKLASTAPLTPEEKKLIKSLNLLTLKPILYAFNGKTGGHNLNEIG
                     DGRAEKAYEYVESIGGQYVHVDARTEHELNDVAEGDKQMFRQELGVQVDGLEELIRGA
                     YRTLGLITYFTTGPKESRAWTVPIGASAPEAGAAIHNDFKDKFIRAEVIGCDTLIADG
                     SYAVAREKGDIRTEGKTYIVKDGDVMVFLHS"
     gene            complement(7367..7954)
                     /locus_tag="PK109_00840"
     CDS             complement(7367..7954)
                     /locus_tag="PK109_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRH24123.1"
                     /translation="MKIYLFLGHPNTDATLASGIADAYEAAAKAAGHEVRRADIGKLQ
                     FDPILHKGYRAIQELEPDLKKVQEDITWADHFVLIYPLWWSSVPALLKGMIDRMWLPR
                     FAFSFIKAPDGKSTMGWHRLLKGRTARVIILLKNFPIIERFLYGNYTSDIVNAVLRFS
                     GFKVRLTEVGNSEALSDKAKASWMKKIEKLARKGR"
     gene            complement(7957..8190)
                     /locus_tag="PK109_00845"
     CDS             complement(7957..8190)
                     /locus_tag="PK109_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24124.1"
                     /translation="MGIQNFLLRQVAKHKMKDVPEGQREQILGMLEKDPNLMKKIGEE
                     IDRRVKKGGENQTKATMEVMKKYRAELAALMQN"
     gene            8293..8718
                     /locus_tag="PK109_00850"
     CDS             8293..8718
                     /locus_tag="PK109_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24125.1"
                     /translation="MTTIESAVVKFSFNDPALISDGMYMETKEGLSQLVIMPEVRVVI
                     EPHRIAANDLPAFFRDTYGFRVVSGLYNSRENRAYVARFVFAATNDHDRGLRHLIGID
                     EAFQLLVEGRTWEVRAEANDTRLWIKCVKPEPVRSRVHA"
     gene            complement(8771..9181)
                     /locus_tag="PK109_00855"
     CDS             complement(8771..9181)
                     /locus_tag="PK109_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24126.1"
                     /translation="MSEDIPLAPEHLGPSHYYGDIVRILFVVAAALLFLSQFIGEPFM
                     TSFSALTLAVILVVAAGLTNPVQAWIHWVNIFLSGLSLILFGAIAIARYRETGTMFGS
                     TAIVMILVLVFLFALYNAIKTLRGTIMRDAPVIR"
     gene            complement(9238..9465)
                     /locus_tag="PK109_00860"
     CDS             complement(9238..9465)
                     /locus_tag="PK109_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24127.1"
                     /translation="MNSGELPPDPFELRQRALHKRIEAVASHPRIAGNDEMLDTLAVF
                     AHNLQLLETDEALDEIEQGIVALEQEVGTAS"
     gene            complement(9469..9720)
                     /locus_tag="PK109_00865"
     CDS             complement(9469..9720)
                     /locus_tag="PK109_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24128.1"
                     /translation="MAAKSFHLTVARVGENLFDGDAVSVTLPGKDGVFVVLANHEAFV
                     TELKIGEAHIEAANGKKHHVEIPHGGIAEISANQATILL"
     gene            complement(9720..>10386)
                     /locus_tag="PK109_00870"
     CDS             complement(9720..>10386)
                     /locus_tag="PK109_00870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121709.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRH24129.1"
                     /translation="GKDVLFFMDNVFRFIQAGSEVSSLLGRVPSAVGYQPTLAEEMGK
                     LQERITSTKKGSITSVQAVYVPADDLTDPAPATTFAHLDGTVTLSRQLASLGIYPAID
                     PLASSSTGLTPEIVGQEHYDVANKVKQVLQRYKDLQDIIAILGIEELSDEDKQTVYRA
                     RKLQKFLSQPFFVGEVFTGTPGQYVPLEETVRGFKEIVDGKHDDKPENAFYMKGGIDQ
                     VTA"
BASE COUNT         2488 a         2699 c         2666 g         2533 t
ORIGIN      
        1 tccgacgcta cttgtcagtt ttctttttcg gcggcgcatc atgagacgcg aacgcggtcg
       61 atgtccgagg actgtgcgca cgatggccaa acggttggaa atccggcggc acttggcgtg
      121 gcatgtcctg ttttcgcgtt gcgaacgcag gcgaatttct cgggccttca ttaggcgtgt
      181 ctgtcatgtg cctactccct gcattaacga gcccctgcag tacggggaaa tagtatctaa
      241 ctatgaaaac gagaggaggt ccagttacgc gagtgcgtag gtaccgctct ttgaacgttt
      301 gaaatgcttt ggatcgttca ggttcacgag aatggtgttg tctttgacgt agcggacctt
      361 cttcacctct tcaagcacgc gttcacggga aagtggaccg aacttgcgga gaatctcacg
      421 gataacgtcg cgtacgacac ctggcttgta tccccactca gcaagtgcgt agagaccacg
      481 gccgacgaga acgaagcgct catccttcat aagctcgtta tgggtggtcg ctacatgtgc
      541 cttcttcgca aagaggcttg caatagcgtt agccacatca cggaaatgca tcggagtgcc
      601 ttcgcgctta atgacgagat acgcataatc gcgaataccc ttggtgcgaa tagcaggggc
      661 gtcagaacga ccccattcag caagcgggtt tgagtcgata ttcttggcaa gagcgagcca
      721 gcgcttgagg atttcttcgt tgcggtatgc cacgtcaaca tccttgagtt cggtaaggaa
      781 acgttcgaat acttcgctct cagaaagcac ttcggtatca gaaagggaag aatagaggcc
      841 tgaaagcgct gcatgcactt tcttcgccgt cgcatgattc acatgccagc gagcatagaa
      901 ttcatcagtc tcacgttcac ggaaaaaagc gctcccaacg accagaagga agcggaagcg
      961 gttcttcgcc ttttcgtccg aagcaagacc tgagaggagg tgatcctcag ggaggatagc
     1021 accctggctc tcaacatatg cgcaaagctc atcgaacgca ggttttgcat ccttgaatcc
     1081 tttggacgca cgcacggctt cgatagccgc cgcctcaatc tgacggacac gctcgcgggt
     1141 aataccccag cgctcaccaa tggcttcaag cgtctcgcgt tcgctcttcg taccgaggcc
     1201 gaagcgaagc gtaagcacct cacgggcacg ttcagaaact tcgccaagga gtttcttgac
     1261 gagagtagtg ctatcaaaag aaggctttac cgcagtgcgg ctttttgtag aaatggcgga
     1321 tttagtagct ttgccgggca ttgtgttgta attataccaa aagggtattt ttttgcaagt
     1381 gggggtgtcc ccttacccta tgactatgtt gataatgcgt ccagggacgt aaatcacccg
     1441 tgaagggggc ttcccttcaa gaagcgccac gacggtaggc aaagcaagcg ccgcctcatt
     1501 cgccgccgcc tcagaggcgt cgggcgcgag gtctacttcc ccgcgccgct ttccgttcac
     1561 ctgcacaatg accgctaccg agtcgcgcac cagaagtgac tcgtccgccg ttggccagga
     1621 agactgatgg acgctccctt ctttcccgag tttcccccac aaatgctcgg caaggtgtgg
     1681 cgcaaatgga gcaagtagca tgaggaactc ttcccacgca tcttgcggca cgttctctag
     1741 ggtctcaagc tcttttacga gcaccatcag cgctgagagt gccgtattga atttgaagcg
     1801 ttcagcatct ccctcaatct ttgccgaagc ggtagcgaat gcgcgctgga gttcgataga
     1861 gacctctgtc gcagtagttt ttgcactaag cgtatagatg cgatcgagga atcgacgcat
     1921 cgcctcaact ccgtctagct tccaagggta gtgccctggc tcgttgtagg ggccaacgaa
     1981 ggcaaggtac atacggacgg cgtccgcccc atatttctcg agcatctcat cgggatttac
     2041 gacatttccc caccgcttgc tcatcttctg accgtcagga cccatgatga gaccccggtt
     2101 atagcgctcc tggaatggtt cgcttacggg aacgagcgaa aggtcatata acgccttctg
     2161 gaagaagcgt gcatagagaa ggtgcatggt ggtatgctcg cttcctcccg agtatcgctt
     2221 cactggaagc cacgtcttca tcagttcatt atccgaaaac tccgtcgtgc tgtttggtgc
     2281 gaggtagcgc aagaagtacc aggaagaatc gacgaaggta tcgagcgtgt cgtactcagg
     2341 cgtccagccc ttgccgaata tgcgctcgac gcgctcatgc aattctttcg aggaagcgag
     2401 tggcgctttc ccggtcgggc taaagtctac atcttcaggc aagagccatg gcatatgctc
     2461 tgctggcacg gtatgggcgc gtccttcagg gtcatacacg atagggatag ggcagcccca
     2521 atagcgctga cgggagataa gccagtcacg gatgcggtag ttggtggtgt gttcacctcc
     2581 tacaaacttc actatctccg gcatggcctt ggtattatcc tttcctgaga atttgccaga
     2641 atttatgagc ttaccatcac cggtatagca ctcaccttct ccttcaacga cttgctgtat
     2701 aggaagctcg tatttctttg cgaattcatg gtcgcgctca tcgtgcgcag ggactgccat
     2761 gaccgcaccc gtgccatacg acgccagaac atagtccgca acatagatag ggatttcttt
     2821 ctgcgtggct ggattcactg ccatgacacc ttcgagtcga acgcctgact tctctttgtt
     2881 ctcactgcgc tcacgctccg ttttacgagc ggtctggtcc ttgtacgcac gtatttcctt
     2941 ttcattgccc accgaggtga gaagtgtatc cacaagcgga tgctcaggag caagcacgac
     3001 ataggtagcc ccgaacaccg tgtcagggcg cgtggtgaat acctctatct tctcttcgcg
     3061 cccctgaagc gggaaccaga gacgagcgcc ttgggagcgt cctatccatg cacgctgtgc
     3121 ttcctttatg tcttcgcgcc agggaagttc gtcgagatct gagagaagac gatcggcata
     3181 gtcctttatc ttcatgaacc attgcgtaag gcgcttttct acgacttcag tgtcgcaacg
     3241 ctcgcactta ccatcgatga cctgctcgtt tgcaagcacc gtcgcatcct tcgggcacca
     3301 tttcacggca gcttctgccc gatacgctaa tccatgttca tagagttttg cgaagagcca
     3361 ctgggtccag cggtagtatt ccggcgagca ggtcaccact tcctttgacc aatcgagaga
     3421 ggcgccgatg gaacgaattt gcgcacgcat cttctcgatg ttcttcaagg tccattctgc
     3481 agggttcgct ccttgcttca tcgcagcgtt ttccgcagga agaccgaacg catcgaaccc
     3541 tatcgggaac agtacattct tccctttcat gcgaagcaac cgtgcgtata tgtcggttac
     3601 ggcatatgcg taccagtggc caatatggag gtcaccggac gggtacggga actcgaagag
     3661 caggtagtac ggctctttgc tcgtgtcctg gagatccgtt tcgtacaact ttgctgcgtc
     3721 ccatttatcc tgggccgcct tttcaattgg cttatgctcg aagcgctcct tgcccatgta
     3781 cttattgagc cttgagaggt gcgtagcgct caggatctat ggttcgctcg tagcagtgct
     3841 cgcctgctgg atgcttccag ttctcatctg gctgcggacc ccatgaggtg tatgcgatgt
     3901 cgcggcctgg atagcttcct tgacctgcgg tgtccggcgt ctcagcacca aaggtgctac
     3961 agagcgagaa ggtaagtggg ccagcaacag agtagctata gctagcaccg gtctctgggt
     4021 ctacagggat aacggaaccg gagagggggt cagcgacttc agcaagcgtg gcgggcaact
     4081 cacccttggt ctgccagtag tagatgatct ggtactggat gttctggagg tcagacactt
     4141 tctgtgcgtc atagcggagc gtacggagct cagacggcgt accgatgata aagaagccag
     4201 caataatcgt cgcaaggacg aggactgcaa cgcccacccc taccaagttt cccttcttag
     4261 aatgcgctac ccagtatcct ttcaaatcag caaggaagtg gaggaagatg ccgagtgcga
     4321 cgaggagtac aacagcaact ttgagaatga agcgttcgct aatctcgcca ccaaggaagg
     4381 tagtgagtgc ggtgatgagg tcgatggcga ccgcagctcc cgcgacaaag agcgtgagca
     4441 cgagtgccca gcgacgcacc cagatctgtg ccttaccagg ttctgtgact atggtgctgc
     4501 gaataatacg gaagagaaca agggtaaggg gaaccagcac gatgagcgtt gccatcgcaa
     4561 cgtgactgcc gctgccgtag gggtctccag aatatgcgag aggatcgggg aatgcgtagt
     4621 ttatgtacgt aaacacgagc gtgagaaagc ccgtgacgct gccgtagagt gcaactatcg
     4681 caccgagcca gagaaagaaa tccttcggag ttgtttttgt gatgggttcc atatcccagg
     4741 attataacac aattaataac tatcttctct cagaagatat acgttacagc gaaagtgaaa
     4801 gcacaacgcc tgagagcact gcagcaattc ctgcaaacgc gacaagggtt tttgcaaaga
     4861 gtgcaattcc ctctttcttc tgtccttcaa gcaacaactg aagcgggact ccgagcataa
     4921 ggtatcccgt gactgccgca gaaaatacaa agagagaaag catgcctagt atcggtgcta
     4981 tcggggttac gggttcagga atgaatttag gaacaaacga aagaaaggca gcaaccactg
     5041 caatgtaagt taccgtagct aacgcattta agaaggggtt tcgggtcatt agaaaagtat
     5101 agcttaggct ggttgtctgg gacgatggtg gaacctttat ctccacttac gcgcccgcat
     5161 gaccatctac gaagaccacc gtctggttgt ctccgacctt ccacatccga acatacagct
     5221 ggcggctttc tggcatttgc ttaatgaacg tggctccgag cgcaagaagg tctgcgacat
     5281 ccacagaaat tgcctgtgca tttgttgttg atcgatattt ctcgcgcgga cgattgaatt
     5341 cctcaatctt acggttaaag tcttcctttg atagagaggg atcagtaagt tcgaccataa
     5401 gattgtatgc ggatggcgag agtactttta tacgcagaac cttttcttcg agtcctttcc
     5461 gttcttgttc gagagaacgg agttgtgtgg gagtaagcgg actattaaga ttcttttcag
     5521 acatatttta agtatacctc ttgctggctg tctgggacgc tattggaaca attatggcca
     5581 atgaagtact gcaagcaggg gcgcaaaaat gacgagtata cctaaaacga cggtacttat
     5641 accgtaaagg taatcattac cataaatact atacttatct gacttcgcta acggttttgt
     5701 tttcgctctc tggtatacct gtaccccaga gtaaatgaac caaagtccca ttggaactat
     5761 caagactaac cagggtaaaa caaaataaat atccatatcc agtgaacaat tatggcacac
     5821 gatgacgagc tggctgtctg ggacgatatg ggacttttct tcgacctact caggattgat
     5881 gatgaaaact gcaacaaaaa aggcgactac cactattaag acggccaagt atacggtttt
     5941 aaccaacttt ttgagtttta gagtttttgg gtccgagtct aatacagtgt cccccttcgt
     6001 aaaataccta agggttttaa atgctttctt gaacttggat agattgggag agagtgctga
     6061 tatgaatcct ggttccccta tctctaccca cttttcgtag tttagcttct tgatacgtct
     6121 gaatagtatc tcttgggtag tccagcacga aagacatgag aggacaataa tagtaatcca
     6181 cacaacctca agattttgag cagttaagtc catttaagta gcataacccg aatggcggac
     6241 taggattcga acctttagct atggaggaac accatcacat ccccatcctt cacgatatag
     6301 gtctttcctt ccgtcctgat gtcacctttt tcgcgtgcaa cagcatagga accgtcagcg
     6361 atgagggtgt cgcaaccgat cacttcagcg cgaatgaact tatccttgaa gtcattgtga
     6421 attgctgcac ctgcttcagg agctgaagcg ccgataggca cggtccatgc acgtgattct
     6481 ttgggtcctg tggtgaaata ggtaatgaga ccaagggtgc gatatgcgcc acgaataagt
     6541 tcctcaagac cgtctacctg gacaccaagt tcctggcgga acatctgctt atcgccttcc
     6601 gcaacatcat tcagttcatg ctcagtgcgc gcatccacgt gtacgtactg gccaccgata
     6661 gactcaacgt attcatacgc cttctcagca cgtccgtcgc ctatctcatt caggttatga
     6721 cctcccgtct taccgttaaa agcgtaaaga ataggtttaa gagtgaggag attcaaggac
     6781 ttaatgagtt tcttttcttc tggggtaagg ggagcagtag aggcgagctt tcctgcgttt
     6841 aatgcttcct caaccttctt aagcgcctca tcttccgcaa ccgcctcttt cataccagca
     6901 cgaatatcac gcgtgagctt ttctcgacgc ttctgcactg catcacgatc agcaagcgca
     6961 agctcgaggt taatcacttc aatgtcgcgc accggatcca cttctccatg cacatgggta
     7021 acatccgggt cttcgaagaa tcgcaccatc tcaagtatgg cgtcagtctc acgaatattt
     7081 gagaggaact ggttccccaa cccttctcct tcggacgcac ccttcacgag tcctgctata
     7141 tccacgaact caatagcggc gggaatagtc ttctcggagc cggagaaggc ggcaagcttc
     7201 tgaagacgct catccggaac ccctaccact cccactgacg gatcaatagt gcagaacggg
     7261 tagttctcgg caggcactgc cgctttggtg agtgcattaa agagcgtgga tttccccacg
     7321 tttggtagac cgacgattcc tatcttaagc atgagtccac actaccttat cggcctttac
     7381 gagcaagttt ctcaatcttt ttcatccacg atgcctttgc tttatccgaa agtgcttcgg
     7441 agttgcccac ttcggtaagg cgaaccttga aaccggagaa gcgaaggaca gcgttcacga
     7501 tatcactcgt gtaattgccg tagaggaaac gttcaataat cgggaagttc ttaagaagaa
     7561 tgatgacgcg tgcggtacgt cccttgagaa ggcggtgcca ccccatggtt gatttaccat
     7621 cgggtgcctt tatgaaacta aaggcaaagc gcgggagcca catgcgatcg atcatgcctt
     7681 ttaggagcgc aggcaccgag ctccaccaca acgggtagat aaggacgaaa tggtctgccc
     7741 aggtaatatc ctcctgtact ttcttaaggt ccggctcgag ctcctgtatg gcgcgatacc
     7801 ctttatggag aatagggtcg aactgaagct tcccgatgtc tgcacggcgc acctcatgcc
     7861 cggctgcttt tgcagcagct tcatacgcgt cggcgatgcc actagcgagc gtggcgtccg
     7921 tattcggatg cccaaggaaa agatatatct tcatgactag ttctgcatca gcgccgcaag
     7981 ctcggcgcga tatttcttca tcacctccat ggtggcctta gtctgattct cacctccttt
     8041 ctttactcga cgatcaatct cttcccctat tttcttcata aggttcggat ctttttcaag
     8101 catgccaaga atctgctcgc gctgaccttc tggcacatcc ttcatcttgt gcttcgcgac
     8161 ctggcggagc aggaaattct gtatacccat atgttccgat tataccatga gcgttatttg
     8221 acaggttctt cacatacgcg ttagataccg atgtacccca tacaacggag gaatagtcag
     8281 atgccaaagg ggatgaccac catcgaatct gcggtcgtta aattctcatt caacgatcca
     8341 gcactgatat cagatggtat gtacatggag acaaaagaag ggctgagtca gttggtcatt
     8401 atgccagaag tacgtgtggt gattgaaccg caccgtatcg ctgctaacga tctccctgct
     8461 ttctttagag acacctacgg gttcagggtg gtgtccgggc tctataactc tagagaaaac
     8521 cgcgcctacg tcgctcgctt cgtgttcgcc gctactaacg atcatgatcg cggccttcgc
     8581 caccttattg gtatcgacga agcatttcaa ctgctagtcg aaggccggac gtgggaagta
     8641 cgcgcagaag cgaacgacac tcgtctctgg atcaaatgcg tcaaaccaga accagtacgc
     8701 tcacgcgttc atgcgtaatg cataacgcga acggcccgcc tcaccggcgg gccgtttcag
     8761 tatcactcct ttaacgaatc accggagcgt cgcgcattat cgtcccgcga agtgtcttta
     8821 tcgcgttata gagcgcaaac aggaacacga gcacgagaat catgactatc gctgtgctcc
     8881 cgaacatagt gccagtctca cggtagcggg caatagcaat tgctccgaag agtatgaggg
     8941 aaagccctga taggaagatg ttcacccagt gaatccaggc ctgcacgggg ttcgtgagtc
     9001 ctgcagcaac gacgagaatg accgcgaggg tgagtgcgga gaagctcgtc ataaacggtt
     9061 caccaataaa ctgtgaaagg aagagtaacg ccgcagcgac aacgaacaag atacgcacga
     9121 tatcaccgta ataatggctt gggccgaggt gttccggggc cagtggtatg tcttcgctca
     9181 tagaataagt atgacactca cctacccttt gacaaggaag aagagtgcgt ctggggatta
     9241 cgaagcagta cctacttcct gttcaagtgc cacaattcct tgctcgattt catcaagcgc
     9301 ttcatccgtt tcaagcaatt ggaggttatg agcgaacacg gcaagtgtgt cgagcatctc
     9361 atcattccca gcaatgcgcg gatgtgatgc gaccgcttca attcgtttat gaagtgctcg
     9421 ctgacgcagc tcaaacggat ccggcggcag ttctccagag ttcatacgct aaaggagtat
     9481 ggtcgcttgg ttggctgaaa tctcagcgat gccgccatgc ggaatttcca cgtgatgctt
     9541 cttcccattc gccgcttcaa tgtgcgcttc accgattttc aattcggtaa cgaaggcttc
     9601 atggttggcg agcacaacga agactccgtc cttacccgga agcgttactg acactgcgtc
     9661 tccatcgaag agattctctc cgacgcgcgc taccgtgaga tggaaagatt tcgcagccat
     9721 tatgccgtta cctgatcgat gcctcccttc atgtagaaag cattctctgg tttatcgtcg
     9781 tgcttaccgt caacgatttc cttgaagcca cgcacggttt cttcaagcgg cacgtactgg
     9841 cccggagtac ccgtgaagac ttctcccacg aagaatggct gggagaggaa cttctggagc
     9901 ttacgagcgc ggtacaccgt ctgcttgtct tcgtcagaga gctcttcgat accaagaatg
     9961 gcgatgatgt cctgaaggtc tttgtagcgc tggaggacct gcttcacttt gttcgcgacg
    10021 tcatagtgct cctgaccaac gatttctggc gtgagaccgg tagaagaaga agcgagcgga
    10081 tcaatagcag ggtaaatacc aagggaggcg agctggcgcg aaagcgttac cgttccgtcc
    10141 aagtgagcga aggtcgttgc tggtgctggg tccgtgaggt cgtctgctgg cacgtacacg
    10201 gcctgcaccg aggtgatgga tcccttcttc gtagaagtaa tgcgctcctg gagcttaccc
    10261 atttcttcag ccaaggttgg ctggtacccc acggctgaag gcacgcgacc aagaagtgag
    10321 gacacttcag aaccagcctg aatgaaacgg aacacgttgt ccatgaagaa gaggacgtcc
    10381 tttcct
//
LOCUS       DAORIN010000011         9893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1175_length_9893_cov_5.046148, whole genome shotgun sequence.
ACCESSION   DAORIN010000011 DAORIN010000000
VERSION     DAORIN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9893
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1175_length_9893_cov_5.046148"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PK109_00875"
     CDS             complement(<1..347)
                     /locus_tag="PK109_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRH24130.1"
                     /translation="MNDLIIIGGGPAGVAAGVYAARKHLKSVLIAEEIGGQSTVSEGI
                     QNWIGTINIAGADLAKALGAHLEAYKSDKLETVIGERVAKLEKSEGGFKATTTGGKSY
                     EAKAVLIASGATRR"
     gene            complement(400..1638)
                     /gene="ftsZ"
                     /locus_tag="PK109_00880"
     CDS             complement(400..1638)
                     /gene="ftsZ"
                     /locus_tag="PK109_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRH24131.1"
                     /translation="MSKRVTPEIETFARIRVVGCGGSGCNAVNHMINSKVKGVEFIAV
                     NSDAQDLHRSLAKRKIHIGKNLTRGLGTGMNPELGRRAAEETRQEIQEALQGSDMVFI
                     TCGMGGGTGTGSSAVVAKIAKEVGALTVAVVTRPFSFEGAQRSEIAERGLADLKKEVD
                     AYLIIPNDKLLSIVEPGTSAKAAFAFADEILRQAVEGVSDIITTPGEINTDFNDIKAV
                     VEGAGPALMGIGIAEGDNRARDAAAAAVNSPLLDVSINGAHGVLFVVAGSEDLGILEV
                     QEAAKVIGESVDKDAKVIFGIMRDEKLKKGQIRIIVIATGFPESSFAEASLPMARPAE
                     RNLFQMPSKDQEEVRGKIYNDMKPEPKPVEEEPKKERPEPIVTARSERRDDKPEIKPV
                     VEDDDSWGGAIPSFLRRNKK"
     gene            complement(1849..2190)
                     /locus_tag="PK109_00885"
     CDS             complement(1849..2190)
                     /locus_tag="PK109_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24132.1"
                     /translation="MIVSSFWYITDKPVGGTTTQCIDAEEAQMAISEPLKKKLREAWV
                     DNDEVTRAINDEEISLLISEMRKSLKTENADFVYAVSRFLSAEMIGPYVRRAKALIAK
                     RDSLELLIKET"
     gene            complement(2187..3365)
                     /gene="ftsA"
                     /locus_tag="PK109_00890"
     CDS             complement(2187..3365)
                     /gene="ftsA"
                     /locus_tag="PK109_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRH24133.1"
                     /translation="MPRRISTGIDIGTHQTKVVVVEEVSVGKGNELRIIGTGLAPSNG
                     MRHGYVVDAEDVAASVKNARRQAENIARVPIKNAFLAVGGISLDETRATGTAVISRAD
                     QEVTDLDLEHALESARQVAEREFTNRRVLHEIPLEFRVDGHKALGDPVGMRGTRLEGD
                     YLFVTCLESHADTLTNAVEAADIEVLDCMASPLAGSYVTLTKDQKMKGCVLANIGAET
                     LSIVVYDENVPVSVKVFPSGGSSVTDELALGFRVSLEDAERIKMGKLSGAMYPKKKVD
                     EIITSRFRSMFNLVDSHLKALGKRGNLPAGIILSGGGSGQGSVADIAKGALALPSRIA
                     DLQVAPDSKVRDATWAVAYGIALWGLTGDTDTPRRGGGNVSLAGATKSLKRFFRQFLP
                     "
     gene            complement(3784..4047)
                     /locus_tag="PK109_00895"
     CDS             complement(3784..4047)
                     /locus_tag="PK109_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24134.1"
                     /translation="MLNAVRVEVRRGKNESAASLIRRFSRRAQGLNLVRMMRKKRYFE
                     RQKSKNVDHKRAMVSGERRAKFNEQVKLGKIDLSAPRGKGRKR"
     gene            complement(4460..5620)
                     /locus_tag="PK109_00900"
     CDS             complement(4460..5620)
                     /locus_tag="PK109_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRH24135.1"
                     /translation="MSTLIIEPTDVLKRAHAVGTYYGFESLAAAAHKNRSNASKSPFP
                     ESLSLDALDPHAREVAGFLKSVRDAGLAPSSGRPHFFWHTNAAAGRAAPKQIVIQFHA
                     LGADRAIADAVLIRAVRSLATDITKLDSRLRLNSMGDRETRGRLARELGSFFRKRGSS
                     LPADCTESAKKDVFAAAELLVNTPEREGMPSPTDHLSEASRKHFESVLEYLEATDTPY
                     ELAPELLSRGGTWNETCFEITAGDRFHAWGSRYNDLAKHFFKSIPSSVGAVIRISTDA
                     VTGGKKQPTIEPQKTSSPRFVFVHIGDEAKRESIKMADALRKARVPMTQVLGVESLTE
                     QMRLADELNPRYLIIMGRKEALERSVILRERATHTETFIPFDGLIDRLRAVA"
     gene            complement(5617..6156)
                     /gene="lepB"
                     /locus_tag="PK109_00905"
     CDS             complement(5617..6156)
                     /gene="lepB"
                     /locus_tag="PK109_00905"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRH24136.1"
                     /translation="MRALKEIFTLVILAVLIVVPIRVFIAQPFVVEGLSMFPTFDDKD
                     YLVVDELSYRFETPERGDVIIFRYPGNPSVFYIKRVIGLPGEKIAIDHGVVMVIKTDG
                     SRETLDEPYVVAEDATYTLNTALGADQFFVMGDNRPKSSDSRVWGALPKEDIIGRAFV
                     RVYPFTDAGFLPGDVTFTQ"
     gene            6244..6840
                     /gene="pth"
                     /locus_tag="PK109_00910"
     CDS             6244..6840
                     /gene="pth"
                     /locus_tag="PK109_00910"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRH24137.1"
                     /translation="MAFVIIGLGNTGKEYEKTRHNAGRMVVELIAKKHDFPAFVLNKT
                     AKALVSNGVIDGEKVTLVLPETMMNLSGKSAMAFVKSPKAAKNLLVIQDELDLPLGTL
                     KMVFDRGSGGHKGVESVMRAIKTKAFTRLRVGISGAGKKHQAKKPSGEEKVVKHVIGK
                     FKPTEEAVLKKSIARAAEASRLFVTEGIEKATMEANTR"
     gene            complement(6837..7211)
                     /locus_tag="PK109_00915"
     CDS             complement(6837..7211)
                     /locus_tag="PK109_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24138.1"
                     /translation="MTNEGEGENKGVVESFKAPVGTPVEIFEERMKIWHEQFSTLIET
                     GADERFQKLLDGLAGDLAMYAKKVSPVALDYMARTFDIKRSEWAQKAAAAEMEDLQEA
                     HAAVSLRYAALASMMNRVRAMP"
     gene            7271..8029
                     /locus_tag="PK109_00920"
     CDS             7271..8029
                     /locus_tag="PK109_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRH24139.1"
                     /translation="MQQLPLSRTRFGEEIVAEFIQPEVSTNKVIIFSAGCPGYPLGKD
                     ELMHKFSRLGYWTISYAYRGTWESGGTFLEYPPSDDILTIMDNLGSFEDSWSGTRHTI
                     HTPEVYLIGGSFGGAATLLASRDPRVKKAITISSVTDWMDQQHTVEPLDFMNEYLPRA
                     FGMAYRGEQEVYRKLTVGDFYNPTHEKKTIDGKKLLLIHAKGDRVVHALPAKAFATEV
                     GAQFVMLDSDDHMGVGYAKEPRVYKHIEKFMKTK"
     gene            8109..8738
                     /locus_tag="PK109_00925"
     CDS             8109..8738
                     /locus_tag="PK109_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24140.1"
                     /translation="MREGEFNRLLRECNAPTQEWNIPAKMLWLAVKSGQTQIHPTEAG
                     LNCLTKSLWTVVSYFSSNGKMYYLYEIERIFANGVSDYRPRFVQLPDREELLPVAFSE
                     SGKKDESDNALIRRAFWEEMRKRISWDRMVVELLRKLETHDSVAHKGLLTSTWGPVVS
                     YMASPSEFASGSEIVTMDERKIITRLGWETEEEMRIRRAAALEKNPSIP"
     gene            complement(8822..>9893)
                     /locus_tag="PK109_00930"
     CDS             complement(8822..>9893)
                     /locus_tag="PK109_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRH24141.1"
                     /translation="HPMAGLIDSIATPPKTGDLVEGTIIALVRGRLYVDLPPFGAGII
                     YGREYLNAADVLRKANPGDQITAKVVDPSGIEGYIELSLKEARQAAIWGEAEAAILAG
                     TVYSVNVEEANKGGLILSWQGIQGFLPASQLAKEHYPRVPEGDKDKILGELGKLVGTP
                     LQVRIITADAKEGKLIFSERTGAEDEEKTSLIDKYVVGDTVEGEVTGAVDFGIFVKLE
                     QGLEGLVHISELDWGLVEDPRALFKVGDKVKVKVIEIKDGKVSLSVKALKENPWKAAA
                     ERYKKGMEVHGVVIKYNKHGALASVEEGVAGLVHISEFASPLELRESLELGKTYPFAI
                     KVFEPKDQRMTLAYTGPKKTKK"
BASE COUNT         2347 a         2614 c         2596 g         2336 t
ORIGIN      
        1 cgtcgcgtag cgcctgaagc tataagaacc gcttttgcct catacgactt ccctcctgtg
       61 gtcgttgctt tgaacccgcc ctcactcttt tcaagcttcg ccacgcgctc cccaatgacc
      121 gtctcaagct tatcgctctt atatgcctca agatgcgcac cgagcgcttt ggcaaggtcc
      181 gctccggcga tgtttattgt tccaatccag ttctggatgc cttcagacac agtagactgt
      241 ccgccaattt cttcagcgat aaggacagac ttcaagtgct tacgtgcggc atagactcct
      301 gcggctactc ctgcgggccc gccgccgatg atgatgaggt cgttcatgtc cggagagtat
      361 aacaaaatcc ccgactgtct gccggggatt tcgttgctgc tatttcttat ttcgtctcag
      421 gaaggatgga atcgctccgc cccagctgtc atcatcttca acgacaggct ttatctctgg
      481 cttgtcatcg cggcgctcgc ttcgtgcggt gacgataggc tcaggacgct ctttcttcgg
      541 ctcttcctca accggcttcg gttcaggctt catatcgtta tatatcttgc cgcggacttc
      601 ttcctggtcc ttagaaggca tctggaagag gttacgctcc gctgggcgtg ccatcggcaa
      661 gcttgcttct gcgaagctgc tctccgggaa gccggtagca ataacaatga tgcggatctg
      721 gcccttcttg agcttctcgt cgcgcatgat accgaagatg actttcgcat ccttgtcgac
      781 cgactcgccg atgaccttcg ctgcttcctg cacttcgagg atgccgaggt cttctgagcc
      841 tgcgaccacg aagagtacgc cgtgggcacc gttgatagag acatcgagaa gcggagagtt
      901 gacggctgca gcagcagcgt cgcgtgcgcg gttgtcgcct tctgcaatac caatacccat
      961 gagggctggg cctgcaccct caaccactgc cttgatgtcg ttgaagtcgg tattgatttc
     1021 tcccggcgtg gtgatgatgt cggagacacc ttccaccgcc tgacgaagaa tctcatcagc
     1081 aaacgcgaac gcagcctttg cggaagtacc aggctcgacg atggagagga gcttgtcgtt
     1141 tgggatgatg aggtacgcgt ccacttcttt cttaaggtcg gcaaggccgc gctcagcaat
     1201 ctcagagcgc tgcgcgcctt caaaggagaa tgggcgagtg actaccgcta ccgtgagtgc
     1261 gccgacttcc ttggcaattt ttgcaacgac cgcagaggag ccggtgccgg tgccaccacc
     1321 cataccgcag gtgatgaaca ccatgtctga accctggaga gcttcctgga tttcctgacg
     1381 cgtctcttca gcagcgcggc gtccgagctc tgggttcatg ccggtgccga gtccacgggt
     1441 gaggttcttt ccgatatgaa tcttgcgctt ggcgagcgag cgatggaggt cttgggcatc
     1501 tgagttgacg gcaataaact ccactccctt caccttggag ttaatcatgt ggttgacggc
     1561 gttgcatccg gaaccaccgc agccgaccac ccgaatccgg gcgaacgttt caatctcagg
     1621 ggtgacgcgc tttgacatag tcaattaggt aaattggccc agtaatagtg ctccgtgcca
     1681 tggagcgaag aacatgcccg cacagtggga tttatcatac tcctgcctca ggagggcgca
     1741 aatgttgcca gaggggagga atttgtgtca taaaacgact gtttataaaa gaaaaacctc
     1801 gcctagtaag cgaggtgagc ggattatgga cctcgctctg gccagtcgtt atgtctcctt
     1861 gatcaggagt tccagcgaat cgcgcttagc gatgagtgct ttcgcccgcc tgacgtatgg
     1921 tccgatcatt tcagcactaa ggaaacgtga gacagcatag acgaagtcag cattctcggt
     1981 tttgagcgac ttacgcattt cgcttatgag cagagatatc tcttcgtcgt taatcgctcg
     2041 cgtaacctcg tcgttatcga cccatgcttc gcggagcttc ttcttgagag gttctgaaat
     2101 tgccatctgc gcctcctcgg cgtcgatgca ttgggtggta gtgcccccaa ccggcttgtc
     2161 cgttatgtac caaaaggaag aaacaatcaa ggaaggaact gacggaagaa acgcttcaat
     2221 gatttggtag cgccggcaag cgatacattt ccaccaccgc gccgcggcgt atccgtgtct
     2281 ccggtgagtc cccagagtgc aatgccgtat gctacggccc atgttgcgtc acgcaccttt
     2341 gaatcaggcg ctacctgaag gtctgcgata cgcgatggga gggcaagcgc gccttttgca
     2401 atatctgcaa ccgaaccttg tccggaaccg ccccccgaaa gaataatgcc agcagggagg
     2461 ttaccgcgct ttccaagcgc tttcagatga ctatcaacga gattaaacat tgagcggaag
     2521 cgcgaggtga tgatctcgtc tacttttttc ttcgggtaca ttgcgcccga caacttcccc
     2581 atcttgatgc gctcagcgtc ttcaagcgag actctaaacc cgagcgcaag ctcatcggtg
     2641 actgaagacc ctcctgaagg aaataccttt actgagactg gtacattctc gtcgtacacg
     2701 actatagaaa gcgtttcggc accgatgttt gcgagcacgc agcctttcat cttctggtcc
     2761 ttggtgagtg tcacataaga gccagcaaga ggggatgcca tgcaatcgag tacttcaatg
     2821 tcagccgctt ccacggcgtt cgtgagtgtg tccgcatgac tttcgaggca ggtaacaaaa
     2881 aggtagtcac cctctaagcg cgtgccgcgc ataccgacgg gatcgccgag cgccttatgg
     2941 ccgtcgactc taaactcaag tgggatctca tgcagcactc gacgattagt aaactcacgt
     3001 tcagcaacct ggcgtgcgct ctcgagtgcg tgctcaagat caagatcagt gacctcctgg
     3061 tcagcgcggg agatgaccgc agtacctgtg gcgcgcgttt catcaagtga gatgccaccc
     3121 accgcaagaa acgcattctt tatcgggacc cgtgcaatgt tctctgcctg gcggcgtgcg
     3181 tttttgacgg acgcagcaac gtcctcagca tctaccacgt atccgtggcg catgccgtta
     3241 gacggagcaa ggcccgtacc aatgatgcgg agctcgttac cctttccgac ggacacctct
     3301 tcgaccacca cgactttcgt ctggtgtgtt ccgatgtcga ttccggttga gattcgtcgg
     3361 ggcattgaga gagctcttac gtacgctaat aaatcgggca cgcagtgtcc gttccttctt
     3421 ctccattgta ggcccgtgtg ggtatccttt caaatgcatg agtgcgtgga taaacaagta
     3481 gccaacaaaa tcgttgtagg aaagttcgta gtcagatgcc tgtttttctg caacattcaa
     3541 acaaataaca atctcaccgc tcttcacgcc ggattcatat gagagcacat tcggtacata
     3601 gtctttaccg cgcagtgatt ggttcagcga cttcgcgcga gcgtttccta caaacacaag
     3661 tgaaatatcc caaccgggaa gtgccgcggc aacagcgtcc gcgtacgcaa aacgaggcct
     3721 agggcgcctc gtcatgttcc gtatatcaat cgtactcatc agaggatgag tgggcgcgct
     3781 aatttagcgc ttgcgtccct tgccgcgtgg tgcggagaga tcaatctttc cgagctttac
     3841 ctgctcgttg aacttagcgc ggcgctcgcc ggaaaccata gcgcgcttgt ggtccacgtt
     3901 cttggacttc tggcgctcga agtagcgctt cttgcgcatc atgcgcacga gattaagccc
     3961 ctgcgcgcgg cgggagaaac gacggattag ggaagccgcg gattcgttct taccgcggcg
     4021 gacctctacg cgtactgcgt ttaacataga taggactgta gcatagaggc tctagggctg
     4081 gcaaggcata tgaaaagggc gcggaacctt cattccgcgc ccctaccaaa agtcctagct
     4141 gagaagcggt ctcagctaag aatgaacgcc ataagcgcga cgattgccgc gcccatgaat
     4201 atgttgccga aggttctctg gccgcgtcct cgtgcgacaa gatagccgag agaacagagg
     4261 ccggcgccga tcagctcggt tcgttcaagg agagaaaagg cgttttcatg cggaaatggg
     4321 gtaaccccca gtccaataac aatcgaagcc atccctgcac ccagcacgaa ataatcgaaa
     4381 agcgcagttt tattcgtagc accactcatt tgccacctcc atggccgtaa aagttttatg
     4441 ccttattaaa atataaatgt caagcaaccg cccgaagtct atcaatgagt ccatcaaacg
     4501 gtatgaaggt ctctgtgtgc gtcgcacgct cacgaagtat cacggagcgt tcaagcgctt
     4561 ccttacggcc catgatgatg agatagcgag gattaagctc gtctgcaaga cgcatctgct
     4621 cggtaaggga ctcaacgcca aggacctggg tcatcggcac acgtgcttta cggagcgcat
     4681 cggccatctt aatgctttcg cgctttgctt catcaccaat atgaacgaac acaaagcgag
     4741 gactcgatgt cttctgtggt tctatcgtcg gttgcttctt tccgccagta acggcgtccg
     4801 tagagatgcg tatgaccgca cccactgaag aagggataga tttaaagaag tgcttcgcta
     4861 aatcgttata gcgagaaccc catgcatgga agcggtcccc ggcagttatt tcaaagcagg
     4921 tttcgttcca ggtgccgcca cgagaaagaa gctcaggcgc aagctcgtag ggagtatccg
     4981 ttgcttcaag gtattcaagt accgattcaa aatgcttacg acttgcttcc gagaggtgat
     5041 cggtagggct tggcatccct tcacgctcag gcgtgttcac aagaagttct gcggctgcga
     5101 atacatcttt ctttgcactc tccgtgcagt cagccggtaa ggaactaccg cgcttcctaa
     5161 agaaggaacc gagctcgcgc gcaaggcgtc cacgcgtctc acgatctccc atggaattga
     5221 gacgaaggcg tgaatcaagt tttgtaatgt cggttgcaag ggaacgcacc gcgcgtataa
     5281 gtactgcgtc agcaatagcg cggtctgctc caagcgcgtg gaattgaatg acaatctgct
     5341 ttggcgcagc gcgtccggca gcagcgttcg tgtgccagaa gaaatgaggg cggcctgagg
     5401 aaggtgcaag gccagcatca cggacgctct ttaagaagcc ggcaacctca cgcgcatggg
     5461 ggtcaagcgc atcaagcgag agactttccg ggaacggtga cttcgatgcg ttgctgcgat
     5521 tcttgtgtgc tgcagcggca agtgactcaa atccataata ggtacccact gcgtgcgcac
     5581 gcttcaagac atccgtaggt tcgatgataa gcgtgctcat tgagtgaaag ttacgtcgcc
     5641 aggaaggaat ccggcatcag tgaatggata gacgcgcaca aacgcgcgac caataatgtc
     5701 ttctttcgga agtgctcccc atacgcgtga gtcagagctt tttggacggt tatcgcccat
     5761 cacgaagaac tgatcagcgc caagcgcggt gtttaaggtg taggttgcgt cttcagcgac
     5821 cacatacggt tcatcaagcg tttctcgcga tccgtcagtc ttaatcacca tcacgacacc
     5881 atgatcaatg gcgatcttct ctcccggtaa gccaatgacg cgcttaatat agaagacgga
     5941 tgggtttcca gggtagcgga agataatgac atcgccgcgc tcaggcgttt caaagcggta
     6001 ggaaagttca tcgacgacta aataatcttt gtcgtcaaac gtagggaaca tagaaagtcc
     6061 ctctacgaca aacggctgcg caataaagac gcgaatgggg actacgataa gcaccgcgag
     6121 aatcacaagc gtgaaaatct ctttaagtgc tcgcattgcg acgcattgta cgcctgccat
     6181 ttttgagcgc aagttttttg gaatgatctc agcaattacg aagcgtgata gaatccaccc
     6241 atcatggcgt ttgtcattat tggccttgga aatacgggta aagagtacga aaagacgcgt
     6301 cacaacgcag gccgtatggt ggtagaactc attgcaaaga aacatgactt ccctgctttt
     6361 gttttaaata aaaccgccaa ggcgcttgtc tcaaatgggg tgatagacgg cgagaaggtt
     6421 acactcgtac ttcccgaaac gatgatgaat ctgtcaggga agtcggcaat ggcgtttgtg
     6481 aagagtccga aggcagcaaa aaatctcctc gtcattcaag atgaactcga tctgccgctc
     6541 ggaacgttga aaatggtgtt tgatcgtggg agcggcggcc ataagggagt agagagcgtg
     6601 atgcgggcta ttaagacaaa agcctttacg cgtcttcgtg ttgggatttc aggagccggc
     6661 aaaaagcatc aggcaaagaa accgtcaggt gaagaaaagg tggtgaagca tgtgatagga
     6721 aagtttaaac ccacagaaga agcggtactt aaaaaatcga tagcgcgcgc cgcagaagca
     6781 tctcgtctat ttgtgactga aggaattgag aaagcgacga tggaagcgaa tacgcgctag
     6841 ggcattgcgc gcacgcggtt catcatggaa gcaagtgccg catagcgaag tgaaacggcg
     6901 gcatgtgctt cctgcaagtc ttccatctcc gctgccgccg ccttctgtgc ccactcactt
     6961 cgctttatat caaacgtgcg cgccatatag tcaagagcaa ccggcgaaac ctttttcgca
     7021 tacattgcaa gatcgcctgc aagtccgtcc aataatttct gaaagcgttc gtctgcaccg
     7081 gtctctataa gtgtgctgaa ttgttcgtgc cagattttca tcctttcttc gaatatctct
     7141 actggcgttc ctaccggagc tttaaaactt tctaccaccc ccttgttttc gccttctcct
     7201 tcgttagtca tgccggtatc ctagcatcct ctacgcagat tcgctcccag catgtatgct
     7261 gtcatacatc atgcagcagc ttcctctttc tcgtacccgg ttcggtgaag aaatcgtcgc
     7321 agaattcatc cagccagaag tatcaaccaa taaagtcatc atcttttctg ctggatgccc
     7381 gggctatccg ctcggcaaag acgaactcat gcataagttt tcgcgcctgg gatactggac
     7441 catttcgtat gcgtatcgcg gcacctggga gagcggcggc accttccttg agtacccacc
     7501 ctctgacgac attctgacca tcatggataa ccttggaagc tttgaagatt catggagcgg
     7561 tactcgccac accattcata ctcctgaggt ctatcttatc ggtggctcct ttggtggcgc
     7621 agcaacgctt ttagcgtcga gagacccacg cgttaagaaa gctatcacta tttctagcgt
     7681 tactgactgg atggatcagc agcatacggt agaaccactc gactttatga atgagtacct
     7741 tccccgcgcc ttcggtatgg cgtaccgcgg cgagcaggag gtgtatagaa agctcaccgt
     7801 gggtgacttc tataacccga cgcatgagaa gaagaccatt gatggcaaga agctcctcct
     7861 catacatgcc aagggcgata gagtggtcca tgcactccca gcgaaagcct tcgctacgga
     7921 agtaggggcg cagtttgtga tgctcgactc tgacgaccac atgggagtgg gctacgccaa
     7981 agaacctcga gtgtataagc acatcgaaaa gtttatgaag acaaagtaac aatcttcata
     8041 attcctcttg cgctattgcg atcgtcccgt agagtaattt tgggggagtt ttctcaagga
     8101 gaactccaat gcgcgaaggc gagtttaatc gcttacttcg agagtgcaat gctccaactc
     8161 aagaatggaa tattccagcg aaaatgttgt ggcttgccgt caaaagcgga cagacacaga
     8221 ttcaccccac cgaggcaggc ctgaactgtc taacgaaaag cctatggacc gtggtgagtt
     8281 atttttcatc aaacggaaag atgtactacc tgtacgagat tgagcgaatt tttgcgaacg
     8341 gcgtttctga ctatcgtccg cgattcgtcc aactaccaga cagagaagaa ctgcttcccg
     8401 ttgcattcag tgagtctggg aaaaaagatg agagtgacaa cgctcttatt cgccgggctt
     8461 tctgggaaga gatgagaaaa cgtatttcct gggatcgtat ggtggtcgag cttcttcgga
     8521 agttagaaac acatgattct gttgcccaca aaggactgct gacctccacg tggggaccgg
     8581 tagtttctta catggcgagc ccttcggagt ttgctagtgg ttcagaaatt gtcaccatgg
     8641 atgagcggaa aataattact cgcctcggct gggaaaccga agaggaaatg agaattagac
     8701 gagcagctgc cctcgaaaag aatccgtcga taccgtaatt ggtatcggcg ggctttttta
     8761 tactcgacat aaccaacaat ggcccgcaga gcgggccatt gttggttctt caatagaaga
     8821 tttatttctt tgtcttcttc gggccggtgt aggcaagcgt catacgctga tcctttggct
     8881 cgaagacctt aatcgcaaac ggataggtct taccaagctc aagcgattcg cgcagctcaa
     8941 gtggggaggc aaactcagag atgtggacga gtccggcaac gccttcttca accgaagcga
     9001 gggcaccgtg cttgttgtac ttaatgacca cgccatgcac ctccataccc ttcttgtagc
     9061 gctctgctgc agctttccat gggttttcct taagggcctt cacggaaagg gaaaccttgc
     9121 catctttgat ctcgatgacc ttcaccttca ccttgtcgcc taccttgaag agggcacgtg
     9181 ggtcttcaac gagtccccaa tcaagctcag agatgtgaac gagaccttca agaccctgtt
     9241 cgagcttcac gaagataccg aagtcaacgg cacccgtgac ttcaccttca accgtgtcgc
     9301 cgacgacata cttatcaatg agagaagtct tctcctcgtc ttcagcgccg gtgcgctcag
     9361 agaagatgag cttaccttcc ttcgcatctg ccgtaatgat gcggacctgg agcggcgtac
     9421 cgacaagctt gccgagttca ccaaggatct tgtccttgtc gccttcgggc acacgtgggt
     9481 agtgctcctt cgcaagctga gaagcaggga ggaagccctg gataccctgc catgaaagga
     9541 tgagaccgcc cttgtttgct tcttcaacgt tgactgagta gaccgtgcca gcaagaatag
     9601 cggcctcggc ttcgccccag atagcagctt gacgagcctc cttaagagaa agttcgatgt
     9661 agccttcgat acctgatggg tcaacgacct tagcagtgat ctgatcacct gggtttgctt
     9721 tgcggagaac gtctgcggcg tttaggtact cgcgtccgta aatgataccg gcgccaaacg
     9781 gtggaagatc cacatagagg cggccacgca caagcgcgat gatcgtacct tcaaccaagt
     9841 cccccgtctt aggcggggta gcaatggaat cgataagacc ggccatcgga tgg
//
LOCUS       DAORIN010000012         9779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1195_length_9779_cov_5.136981, whole genome shotgun sequence.
ACCESSION   DAORIN010000012 DAORIN010000000
VERSION     DAORIN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1195_length_9779_cov_5.136981"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..179)
                     /locus_tag="PK109_00935"
     tRNA            complement(104..179)
                     /locus_tag="PK109_00935"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Lys,seq:ctt)
     gene            223..307
                     /locus_tag="PK109_00940"
     tRNA            223..307
                     /locus_tag="PK109_00940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:257..259,aa:Leu,seq:tag)
     gene            complement(359..613)
                     /locus_tag="PK109_00945"
     CDS             complement(359..613)
                     /locus_tag="PK109_00945"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24142.1"
                     /translation="MDTAISGFIEGIQREILTPLITLISLAAFVLFVWGVVEFVRNAG
                     NEEKRKVGQQHMIWGIIGLVIIFGANAIITMITATLGLPN"
     gene            complement(660..1019)
                     /locus_tag="PK109_00950"
     CDS             complement(660..1019)
                     /locus_tag="PK109_00950"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRH24143.1"
                     /translation="MKKLYVLALLAFATVPMIASAQVRSIQDAAGVVTNIINGVLVPL
                     VFAIAFIVFIWGIFQYFIAGGHDEEAKEKGKSLMLYGLIGFFLMVSVWGLVNILVGTF
                     NLQNNAPTFIPGAPGPR"
     gene            complement(1124..1576)
                     /locus_tag="PK109_00955"
     CDS             complement(1124..1576)
                     /locus_tag="PK109_00955"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRH24144.1"
                     /translation="MPLGNFIVWIIGFINHVLVPFIFAIAFIVFIWGIFQYFIAGGAD
                     EEKRKEGKKLALYGFIGFFLMISIWGIVNLLVNSTGFDNKSRPALPTFGDVGGAGGGF
                     GNNKGGTVLTCSPESEYSPAGLSPDECCKRAFGPESSFNSSTGCTNAN"
     gene            complement(1616..1858)
                     /locus_tag="PK109_00960"
     CDS             complement(1616..1858)
                     /locus_tag="PK109_00960"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24145.1"
                     /translation="MSFAEFVDGKIVPLIDGAVIPLLYALAFIFFLIGVARFFFADGD
                     ESREKGKQFALWGIIAFFVLFSVWGIVKLLLSVIQQ"
     gene            complement(1855..2490)
                     /locus_tag="PK109_00965"
     CDS             complement(1855..2490)
                     /locus_tag="PK109_00965"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRH24146.1"
                     /translation="MKYYFSALVLLLALFVVATPTYAVECEAPAVACVANGVEFCSQG
                     GVSGCHAQNGEVIQSAGTTQTEGQTSPSTQSVTCSPACASYQHCELIASLGEGRCVNN
                     QNSDGSFQTSENPNRTSATNDGNGTFKNPLKAGSLTELLNAVLKGIVQLGSILLVLAL
                     VYVGFLFVAAQGAEEKIRDARNALLWTVIGGLILLGATAISEVITATVSNI"
     gene            complement(2493..3797)
                     /locus_tag="PK109_00970"
     CDS             complement(2493..3797)
                     /locus_tag="PK109_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRH24147.1"
                     /translation="MKELRPFQIVLLAIFGLGALIGLYVFATFGGFGGSKVQVGEVLI
                     WGTLPSEGVNAGIDALAQAQTEYAKVTYVEVSEYTFDSDLAEALASGAGPDLVIVSQE
                     MLSDEKNKLDVIPFTSISERTYLDSYLPIFELFLTPDGTYGLPLVVDPLVLYYNRSIL
                     ASAGIASAPSTWEAVSGLAPSITKRTDGGAVSRSLISLGEYSNVMNARGILSLLLLQA
                     GSPITEVGQQGMRSTLADSPESAFGTSPAQSAVNYYAQFADPAKTLYSWNRSLPNSRS
                     AFIAGDLVLYPGYASEQPFLSEANPNLDYDMAPIPVPATLSNRLTYGKAYAFAIPKAS
                     ANRSGAYAAANALSASNIAATIAKGVGMAPARRASLSAPSNDKYATVFYREALVARGW
                     LSPAPSATDAIFSAMISDITSGRRNVEQALGVASRAITEEIQ"
     gene            complement(3810..4340)
                     /locus_tag="PK109_00975"
     CDS             complement(3810..4340)
                     /locus_tag="PK109_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRH24148.1"
                     /translation="MNFADPKANVLQMGLREGMKVADLGVGSGHYALAAAAIVGSTGR
                     VYAVDIQEDHLAPLQNEARARGLRNIETIWGDFEKLGGTMLKDKALDAVIISNTLFQL
                     ENAAAAIQEMKRILASGGKLLVVDWAGEYNGIGPSKERIVPEQKAEELFITGGFHKVK
                     SFRGGPHHYSILFTAP"
     gene            complement(4400..6871)
                     /gene="gyrA"
                     /locus_tag="PK109_00980"
     CDS             complement(4400..6871)
                     /gene="gyrA"
                     /locus_tag="PK109_00980"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRH24149.1"
                     /translation="MAKKVVPTEDAPVTAANVIAQSITGEMRSSYLDYAMSVITSRAL
                     PDVRDGLKPVHRRILYAMRMTGLTPSAKFRKSATVVGEVLGSYHPHGDASVYDAMVKL
                     AQDFSTRYPLVAGQGNFGSIDGDPPAAYRYTEAKMSRLADEMLRDLEKETVLGAPNFD
                     GSKQEPTVLPAAAPNLLLNGTLGIAVGMATNIPPHNLREVVEATMHLIDNEEATTDDL
                     LEFVKGPDFPLGGIAFNQSDIRHAYSSGKGPVVCRGVAEIIEDKKGSSIIITSIPFRV
                     NKADLITRIADLVRDKKLEGIKDLRDESAEDIRVVVELKGTAHPQAVLNNLFKHTELE
                     TTFHYNMLALVDGVPQTLSLKGMLEEFVKHRRDVVKKRTEYDLRKAEEREHILLGLSK
                     ALDHIDEVIAIIKKAKDVPTADLELQKKFKFSERQTAAILAMRLQTLAGLERKKIEDE
                     LKSIQELIAKLKKILSSAKNILAVVRAELAELMEKYGDDRRTKIIKGGVKNISVEDLV
                     PDEESVLVITAGGYVKRTNPSEYKAQKRGGVGVIDMSTKEEDVVTHFLAASAHAELLF
                     FTDYGKAYQTKMYEIPEGKRATKGKSIMNFLPLAADEKVTSVLAVPKGAARDAMSVFF
                     VTEEGVVKKVEAKNFADVRRSGIIAISLKGKDKLVSAHLSGDGDSVSIVTSKGQSIRF
                     DEADVRAMGRTAAGVRGMNVRKGDTVVSAEVVPAGAKSGSLLIIMSKGYGKQTELSEY
                     KIQGRGGSGIKTADVTAKTGEIIGAKVLIGKEEMTDEELVVISKKGQVIRCKADEIPS
                     LSRATQGVRIMKMREGDSIASMVAL"
     gene            complement(6893..7249)
                     /locus_tag="PK109_00985"
     CDS             complement(6893..7249)
                     /locus_tag="PK109_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24150.1"
                     /translation="MHLRDYLEHLKSKPEDVRRRIALGSSLGITGVVAIGWMGALGAS
                     GTLALSVPVATETKNGFAVAAEETKTGFSSLLGAASAFNSGTTGAGSLTIVGERASST
                     LDTNTDTTEGKTVIPF"
     gene            complement(7254..7895)
                     /locus_tag="PK109_00990"
     CDS             complement(7254..7895)
                     /locus_tag="PK109_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRH24151.1"
                     /translation="MVITHHGGQCFKVTFGDLTLVFDPISKGGTLPGVRFGADIALVS
                     RQHPDMNGVAEVTYGEKEPFAITGPGEYEYGGVTVQGFLTKSEYGLKKGDTVGVNTIY
                     SVKLEGMTLVHLGALSDTSLPADAKEAIDEIDVLFVPVGGDGVLDAAKAAKLTTMLEP
                     RIIIPMHWSGMGEAKALEAYLKEEGGASEKTDKLTLKKKDALDKDGDIIVINP"
     gene            8383..8532
                     /locus_tag="PK109_00995"
     CDS             8383..8532
                     /locus_tag="PK109_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24152.1"
                     /translation="MKKKLLGHVSHIALAAMAAKSATDDLKTGQQQPLNGANDQDDAI
                     GDRPA"
     gene            8747..8881
                     /locus_tag="PK109_01000"
     CDS             8747..8881
                     /locus_tag="PK109_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24153.1"
                     /translation="MLKEKTELVGFAMTQIGQGHSSATTALSATNKHRRRAVSGTQDG
                     "
BASE COUNT         2321 a         2594 c         2594 g         2270 t
ORIGIN      
        1 cgagttagtc agggtgagtg gtaaaatggg ttcgagcgga gttgtatata ctcacacggg
       61 tagatagaaa aaggacctgg aatattccag gtcttttttc ttttggtgcg cctacctgga
      121 ctcgaaccag ggaccatatc cttaagaggg atctgctcta ccaactgagc tataggcgca
      181 atgtttggaa gaatagcgcg gagagtatac tctgacaaga ctgctgaggt ggcggaattg
      241 gtatacgcac cacgcttagg acgtggcggg gaaacccatg gaggttcaag tcctctcctc
      301 agcaccaaaa agacaacgcc cgcggcatgc cgcgggcgtt gtctctattt cccatctcct
      361 agttcggaag cccgagcgtg gccgtaatca tcgtaatgat ggcgttggcg ccgaagataa
      421 tcacgagacc aatgatgccc cagatcatgt gctgctgccc tacctttcgc ttttcttcat
      481 tgcccgcgtt acgaacgaac tcaacgacgc cccacacaaa gagtacgaag gcagccagtg
      541 agataagggt gatgagcggg gtgagaatct cacgctgtat gccttcaatg aatcctgaga
      601 tcgcggtgtc catagtaagt gagtgaaaag cccgcttgga gcgggctttt caagattctt
      661 tagcgagggc ccggagcgcc ggggatgaac gttggcgcgt tgttctgaag gttgaacgtt
      721 ccgacgagga tgttgacaag accccagact gaaaccatga ggaagaagcc aataagtccg
      781 tagagcatga gggactttcc tttctccttt gcctcttcgt catgaccacc tgcaatgaag
      841 tactggaaga taccccaaat gaatacgatg aaggcgatgg cgaacacgag cggcaccaaa
      901 acgccgttaa tgatgttggt cacgacgcca gcggcatcct ggatgctgcg cacctgtgcg
      961 gaggcgatca tcggaacagt ggcgaaggcc aagagtgcga gcacgtagag ttttttcatg
     1021 cgattaggtt atctgcgttg gtagagctaa tggctatagg atagaccgct agaatatggc
     1081 gtcaatagag gttgggtgct cgtgtggaaa acatccacac tatttaattg gcattcgtac
     1141 aaccagtact tgaattgaag gacgactctg gaccgaacgc ccgcttgcag cattcgtctg
     1201 gactaagtcc tgcagggctg tattcgcttt caggagagca ggtaagcacc gtacctccct
     1261 tgttgttacc gaagcctcct cctgccccac ccacgtcgcc aaaggtagga agtgctggac
     1321 gcgacttgtt atcaaacccg gttgaattca cgagtaagtt cacgatgccc cagatggaaa
     1381 tcatgaggaa gaatccgatg aatccataga gcgcaagttt ctttccttct tttcgctttt
     1441 cttcgtcagc gccgcctgca atgaagtact ggaagatacc ccaaatgaat acgatgaagg
     1501 caatcgcgaa tatgaatgga acgagcacgt gattaataaa cccgatgatc cacacgataa
     1561 agtttcctag aggcatatac gtgaagggta gcagtacggt atgagagcgc aactgctact
     1621 gctgaataac ggagaggagg agctttacga tgccccagac gctaaagagt acgaagaacg
     1681 cgatgatgcc ccaaagcgca aactgcttcc ctttctcacg actctcatct ccgtctgcaa
     1741 agaagaagcg agcgacgccg ataagaaaga agataaaagc aagggcgtac agaagcggaa
     1801 tgaccgcgcc gtcgataagc ggcactatct tcccatcaac gaattctgca aagctcatat
     1861 attacttaca gtagcggtaa taacttcaga aatcgcggtt gcaccaagca agataagtcc
     1921 gccgataact gtccacagta atgcattgcg cgcgtcacgt atcttttctt cagcaccctg
     1981 cgcggcgacg aagaggaacc cgacatatac gagggcaagg acaaggagta ttgagccaag
     2041 ctgcacaatc cctttaagaa ccgcattcag aagctcggtg agagagcctg ctttgagagg
     2101 atttttaaag gtaccgttac catcattggt agcagaggta cggtttgggt tttcgctcgt
     2161 ctgaaaagat ccgtctgagt tctggttgtt aacgcaacgc ccttcgccga gtgaggcgat
     2221 aagttcacag tgctgatagc tcgcgcatgc tggagaacag gtgacagatt gtgttgaagg
     2281 tgaggtttgg ccttcagtct gcgttgttcc agcgctttgt ataacttcac cgttttgtgc
     2341 atggcatccg ctcacgccac cttgtgaaca aaactctact ccgttcgcta cgcaggcgac
     2401 tgctggtgcc tcgcactcaa ctgcataggt cggagtcgcg acaacaaaca acgcaaggag
     2461 cagcacgagc gcagaaaagt aatacttcat agttattgga tttcctcagt gattgcccgt
     2521 gaagcgacgc ctagagcttg ctcgacgttt ctacggcccg aggttatgtc actaatcata
     2581 gcggagaaga tagcgtcagt agccgacggt gcgggggata accagccacg cgccacgagc
     2641 gcctcccgat agaagacggt ggcgtattta tcattcgagg gagcagaaag ggatgctcgg
     2701 cgcgccggcg ccataccgac gcccttggcg atggtggcag caatattcga tgcagagagc
     2761 gcatttgcag cagcgtacgc gcctgatcgg tttgcagacg ccttagggat agcaaacgca
     2821 tacgcttttc cgtacgtgag tcggttactt aaggttgctg ggaccggaat aggcgccatg
     2881 tcgtagtcga ggttcgggtt agcttcagaa agaaacggct gttcagacgc gtagcccgga
     2941 tagaggacga ggtcaccggc gatgaacgca gaacgcgaat tagggagcga gcgattccat
     3001 gaatagagcg tcttcgcggg atcagcgaat tgcgcgtagt agttcactgc cgactgtgca
     3061 ggactcgtgc caaacgcact ctcgggagag tcagcaagcg ttgatcgcat tccctgctgc
     3121 cccacctcag taatggggct tcctgcctga aggaggagga gcgagagaat accgcgggca
     3181 ttcatgacgt tgctgtattc cccaagcgat atgaggctac gagagaccgc gccaccatca
     3241 gttcgtttag tgatggaagg cgcaagacca gagaccgcct cccaggttga aggcgccgac
     3301 gcgatacctg ccgaagcgag gatggagcgg ttgtagtaaa gaacgagagg atcaacgacg
     3361 agtggcaatc cgtacgtgcc gtcaggagtc aggaaaagct caaatattgg aagataagaa
     3421 tcgaggtagg tgcgttcaga tatgctcgtg aaaggaataa cgtcgagctt attcttttca
     3481 tcgctcagca tttcttgcga gacgataacc agatcgggac ccgccccaga cgccaacgct
     3541 tcggcaagat cgctatcaaa ggtatattca gatacttcaa cgtaggtgac tttcgcgtat
     3601 tcagtctgtg cctgggcaag cgcatcgatg ccggcgttaa cgccctcaga cggtaacgtg
     3661 ccccaaataa gcacttctcc tacctgtacc ttagaaccac cgaatcctcc gaaggtggcg
     3721 aacacataga gtccgataag ggcaccaaga ccaaagatcg caagcaagac tatctggaag
     3781 ggacgaagtt ctttcatacg caaagtgtac tatggtgccg tgaataaaat ggagtaatgg
     3841 tgcgggccgc cgcggaagct cttcacttta tgaaagccgc cggtgatgaa gagctcttcg
     3901 gctttctgtt caggcacgat gcgctcttta gaaggaccga tgccattgta ctcacctgcc
     3961 cagtcaacga ctaagagctt cccgccggac gcaaggatgc gcttcatctc ttgaatagca
     4021 gcagcggcat tctcaagctg gaagagggtg tttgagataa tcaccgcatc aagcgcctta
     4081 tctttgagca tggtgccgcc gagcttctca aagtctcccc atatcgtttc aatatttctg
     4141 aggccacgcg ctctcgcttc gttttgaagc ggggccaaat gatcttcctg tatatcaacg
     4201 gcgtatacac gtccggtact gcccacaatg gccgcagccg cgagcgcgta gtggccggag
     4261 ccgacgccaa ggtctgctac tttcattcct tcacggagtc ccatctggag tacattcgct
     4321 ttcgggtcag caaagttcat cccttaaaaa tagcatggcc acgcagaggc gtggccatga
     4381 attaacacgc tatgaaattt taaagggcga ccatcgaagc gatggaatcg ccttcgcgca
     4441 tcttcatgat gcgcacgccc tgcgtcgcac gggacaaaga tggaatctca tctgctttgc
     4501 agcggatgac ttgtcctttc tttgaaatga cgacgagttc ctcatcggtc atttcttcct
     4561 ttccaattaa tacctttgcg ccgatgattt ctccggtctt tgcagtgacg tctgcggtct
     4621 tgatgccgga gccgccacgg ccttggatct tgtattcaga aagctcagtc tgcttaccgt
     4681 agccctttga catgatgatg aggagtgaac cgctcttggc gcctgcaggc accacctcag
     4741 ctgagaccac cgtgtcaccc ttcctgacgt tcataccgcg caccccggct gcggtgcggc
     4801 ccatggcacg tacgtctgct tcatcaaagc gtatggactg acccttactc gtaacaatag
     4861 agactgagtc accgtcgccc gaaaggtgcg cggacaccag cttatctttg cccttcaagg
     4921 aaatagcgat gatgccggaa cggcgtacat cagcgaagtt cttcgcttcg actttcttca
     4981 cgacaccctc ttcggtcacg aagaacacgc tcatcgcgtc gcgcgcagca cccttcggta
     5041 ccgcgagcac gctggtgacc ttctcatccg ctgcaagcgg caagaagttc atgatgctct
     5101 ttcccttagt cgcgcgcttt ccttccggaa tctcgtacat cttcgtctgg tatgccttgc
     5161 cgtagtcagt gaagaagagg agctcggcat gagcagacgc tgcaaggaag tgcgtcacta
     5221 cgtcttcttc tttggtagac atgtcgataa cgccgacgcc accgcgcttc tgcgccttgt
     5281 actcgcttgg gttcgtgcgc tttacgtagc caccagcggt aatcacgagg acactctctt
     5341 cgtcaggaac aaggtcctct acagagatgt tcttcacgcc acctttgatt atctttgtgc
     5401 gacgatcatc cccatacttc tccatgagct cggcaagctc ggcacgaacc acggcaagaa
     5461 tgttctttgc ggaagagaga atcttcttca attttgcgat aagttcctgg atagacttga
     5521 gctcgtcttc aatcttctta cgctcgaggc cggcaagggt ctgaagacgc attgcaagaa
     5581 tagcggcagt ctgacgctct gagaatttaa acttcttctg gagttcgaga tcagcggtag
     5641 gtacatcctt cgctttctta atgatagcga taacttcatc gatatgatcg agtgccttgg
     5701 aaagacccaa gaggatgtgc tcacgctctt ctgctttcct gagatcgtac tcggtgcgct
     5761 ttttgacgac gtcacggcga tgctttacga actcttcgag catgcctttt agtgaaaggg
     5821 tctgcggcac gccgtccaca agcgcgagca tgttgtagtg gaaggtagtc tcaagctccg
     5881 tgtgcttaaa gaggttgttg aggacggcct gcgggtgcgc cgtacccttg agttcaacga
     5941 cgacgcgaat gtcttcagcc gactcatcgc gaagatcctt gatgccctca agcttcttat
     6001 cacgcacgag gtccgcgatg cgggtgataa ggtccgcttt gttcacgcga aatggaatgg
     6061 acgtgatgat gatggaactt cccttcttgt cttcaataat ctctgcgaca ccgcggcaca
     6121 ccacgggtcc tttgccagaa gagtaggcgt gacgaatatc tgactgattg aaggcgatac
     6181 cgccgagtgg gaagtccgga cctttcacga actcaagaag atcatcggta gttgcctctt
     6241 cgttatcgat gaggtgcatc gttgcctcta ccacttcgcg caggttgtgc ggcggaatgt
     6301 tggtggccat acccactgca atacccaagg taccgttcaa aagaaggttc ggtgcggctg
     6361 ccggaagcac cgttggttcc tgtttgctgc catcgaagtt tggcgccccg agtaccgtct
     6421 ccttctcaag gtcgcgaagc atctcgtctg cgaggcgtga catctttgct tcggtgtagc
     6481 ggtaagcggc gggcgggtct ccgtcaatgg aaccgaagtt accttggccg gctaccagcg
     6541 ggtagcgggt ggagaagtct tgcgcaagct ttaccatcgc gtcatacacg gacgcatcac
     6601 cgtgtgggtg atagctaccg agcacctctc ccaccaccgt tgccgactta cggaactttg
     6661 ccgacggcgt aaggccggtc atacgcatcg cgtagagaat gcggcggtgc actggcttta
     6721 atccgtcgcg cacatcagga agcgcgcgac tcgtgatgac ggacatcgca tagtcaaggt
     6781 acgacgaacg catctcgcct gtgatggact gagctatgac gttcgctgcc gttaccggag
     6841 cgtcctctgt tggtactact ttcttagcca tgaaattact aaccgatact tattaaaaag
     6901 gtatgacggt cttcccttca gtcgtgtccg tattcgtatc gagcgtcgaa gaggcgcgct
     6961 cacctactat cgtaagagag ccggctccag tagttcctga attaaaggca cttgccgcac
     7021 ccaagagact ggagaatcca gttttagttt cttctgcagc tacagcaaaa ccattcttgg
     7081 tttcggtagc aacaggcaca gagagtgcaa gcgtgcccga ggcaccaagc gcccccatcc
     7141 acccaatggc aaccacacca gtgataccga gggaggaacc gagcgcgata cgacgacgaa
     7201 cgtcctcggg ctttgatttg agatgctcta ggtagtcgcg taggtgcata ggcttacggg
     7261 tttatcacaa ttatatcacc atccttgtca agtgcatcct tcttcttgag ggtgagcttg
     7321 tctgttttct cagaagcgcc gccctcttcc tttaagtaag cctcgagtgc tttggcttct
     7381 cccatgccgc tccagtgcat cggaatgatg atgcgcggct caagcatcgt cgtgagcttt
     7441 gctgcctttg cagcgtccaa caccccatcc ccgcctaccg gaacaaagag tacatctatt
     7501 tcatcaatgg cttcttttgc gtccgcagga agcgaggtgt cagagagcgc tcccaagtgc
     7561 acgagcgtca taccctcaag cttcacacta tatatagtgt tcacgccaac cgtgtctcct
     7621 ttcttaagcc cgtactcgct cttggtgagg aatccctgga ccgtgacgcc cccatactcg
     7681 tattcaccag gaccggtgat agcgaatggt tctttttcgc cgtaggtgac ttctgcaaca
     7741 ccattcatgt cagggtgctg acgcgacaca agcgcgatat ccgcaccaaa acgtacgcca
     7801 gggagcgtgc ctcccttgga gattggatca aagaccagag tgaggtcccc aaacgttact
     7861 ttgaagcact gtccgccgtg atgggtgatg accatgttgt cttcatttta tcataattga
     7921 caggtatttt cctgctagcg ttttaaagag aaaaggctat agtcacagcg tgtcggtctc
     7981 ggtggcttcc atagcactaa caccgggcga caccgcagct agagggcaag cgtttgctct
     8041 ctccactatg gccttaagcg aaaaggacca caggccatga agtatgctga atatcgaagt
     8101 actatcctgt ttctcttaaa gccggggtga cggcacgatg agagcataga gaaacttcgg
     8161 gggccgcgca gtaatcgtcc ctattttccg cttggatctc acgccgagct gattgacggg
     8221 aaaggtttat tgcgcggccc tttctctttt tatacccact gctctcttgc agattttata
     8281 cagaggcgta ggctccaagt actgccaccg gagggacttg aaaatcctca ccggagtaga
     8341 ccttcaacct acagcgtgca agcgctgaga tgaggaaatg tgatgaaaaa gaaactgttg
     8401 ggacatgtgt cccatatcgc tcttgccgca atggcagcga agtcagcgac cgacgatttg
     8461 aaaaccggcc agcaacaacc gctcaacggc gcgaacgacc aagacgatgc gatcggcgat
     8521 cggccggcgt agtcgactag gcgacatgtg aggtgcgggt gggccctcgg cccaccccac
     8581 caaacaaaag gtcactcttc ggagtgactt ttttaaattt tatgcacccc accctattgt
     8641 gcctttatga aggagtggta tcgtccccct accggtcgca agatactttt cttgctactt
     8701 gcgcccggga tttccgtaac tctcactcag attgaggaga aggaatatgt tgaaagaaaa
     8761 aactgaactt gttggcttcg cgatgacgca aatcggccaa ggccactcga gtgccacgac
     8821 cgcattgtcc gccacgaaca agcaccgccg ccgcgccgtt tccggcacgc aagacggctg
     8881 aacgaagatg attccggtat aggtacctga tacggcctac tactggacga caatggaatg
     8941 ggggcgtcta cttcggtaga cgcccttttc aattaatcga tttgatattt tcaataccac
     9001 cagttatggt gaccttctcg ggaagtcctc actcgtgttc ctctcccgga ctgagcgagt
     9061 gattgtgacg cctgagcgca ggggcggcta cggcagcata taggccgccc ctgcatcagt
     9121 tttagcttga cggttggtat cctgacgtcc catgcccgcc aaccgtccgg tgcggcgggg
     9181 gaggaaggac tcacccgcca ataccgctct ttcttaggaa cgcacgacgc gttcctttgc
     9241 tgtttttata ctttgttgta cagtgcctcg gtcaccacgt tgcggtgtcg gagaaggagg
     9301 gaagcatgaa aggctttttg gccaccatgt aacaactctg ttgtgaatat gccggtgaac
     9361 cggcactagc atcttgcgga actctgcacc gtcttagcga tatactgcgg acacgaaagc
     9421 ccgtaagaca catgaagcgg cctctctcat tccctcggaa tgtgacgccg cttcaaccct
     9481 tttattcccc accctcctgt gctaaagtct ccccatcaag agcgcgtgcg taaggcgcaa
     9541 gcgggctaac ccaagggctc tgaaaagagc cattgaaccc gcggcgcaga cgctctcgat
     9601 ccaattacat acatgcccga agaaacaacc gcacccgctg cggataaggt taaagttgaa
     9661 cccgttacca tagtgcttga tccaagccat ccgatggccg gtcttatcga ttccattgct
     9721 accccgccta agacggggga tttggttgaa ggtacgatca tcgcacttgt gcgtggtcg
//
LOCUS       DAORIN010000013         9065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1334_length_9065_cov_5.906992, whole genome shotgun sequence.
ACCESSION   DAORIN010000013 DAORIN010000000
VERSION     DAORIN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1334_length_9065_cov_5.906992"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PK109_01005"
     CDS             complement(<1..292)
                     /locus_tag="PK109_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRH24154.1"
                     /translation="MTVDRIRTFRTTVWKYYRTHGRHDLPWRKSKDPYKILVSEVMLQ
                     QTQVERVKAYYLAWLERFPNIEALAQASLADVLRMWQGLGYNRRAKMLHEAAK"
     gene            complement(404..1090)
                     /locus_tag="PK109_01010"
     CDS             complement(404..1090)
                     /locus_tag="PK109_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRH24155.1"
                     /translation="MSQPIAVFDIDGTIFRSSLFIELVERLVDKGILPKEVREAYVDA
                     QEKWLDRRGDYDTYIEKMITAFRGNLKGVSYEEVAFIAGEIIDEKKHRLYRYTRDMVR
                     DLKAKGYYLLAISHSPRFIVEGFGHELGFDKVYGFFYETGPSGNFTGNVEDHDLIRNK
                     AAVLERAARKAGLSLERSIGVGDTESDIPMLELVEIPLAFNPNKALYEHAQKRGWRVV
                     VERKDVIYEI"
     gene            complement(1489..2154)
                     /gene="nth"
                     /locus_tag="PK109_01015"
     CDS             complement(1489..2154)
                     /gene="nth"
                     /locus_tag="PK109_01015"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRH24156.1"
                     /translation="MASSHAERKARAARIVRYLKRAYPSPETELNYQTPFQLVASVML
                     SAQTTDKQVNKVTETLWKKYKTVNDFANAKYATFDKEIASVSFHRNKAKAIIGAAKMI
                     RDEFDGKVPKTEAELVRLPGIAYKTAHVVLGELHGIYEGIPTDTHVRRFALRFDLTDR
                     TDLARISKDLETLIPKKDWKYVNNGFVLYGRYVCPARPHDCETHPLTKIWPPAAKRWP
                     KSK"
     gene            complement(2158..3291)
                     /locus_tag="PK109_01020"
     CDS             complement(2158..3291)
                     /locus_tag="PK109_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRH24157.1"
                     /translation="MTARALAALLLSSVLPFFALAAEEPNQTLVVSETPGGNSYLAGT
                     DISVDTALPADLMALATSLRISAPVAGDVLALAGSVLVQAPVTGDVRAAGGTIRSTGD
                     VSGELALFGGTVIVSGSAGEIRAAGATVELRSGAHGPVTAYGANILLSGEFDGNVRVV
                     ASDHVTLAEGTIIHGTFDYNAPQEVSIPASAVIDGGANYIGSSSFLPTAQEAETFALA
                     GIGIFFIVRLVAAILAAGLVAGLFPVFGQRVAEEALMRGPKRFLLLALLGFAVIAATP
                     VLLLLLVASFVGIGIAALIGALYLLLLLLAYLYAAVLTGSALMRTLLKRTDVTWKGAV
                     LGMLALGLIGVIPALGLFIAFALSSAALGAILVIFYRFAFKAN"
     gene            complement(3296..3751)
                     /locus_tag="PK109_01025"
     CDS             complement(3296..3751)
                     /locus_tag="PK109_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRH24158.1"
                     /translation="MTRRLLAALLILLALLGITDSWYLASHAATDTALVCDIGAGLDG
                     CNTVAQSPYSQFMGIPLAYFGVAFYTVLLILGALAFCFSPRRLLYKGIFALSVASALA
                     SVCFLFIQFFLIQAVCVYCIVSAVLAFLSVPLSYLLLKRFAPQLPVVIP"
     gene            3778..4200
                     /gene="msrB"
                     /locus_tag="PK109_01030"
     CDS             3778..4200
                     /gene="msrB"
                     /locus_tag="PK109_01030"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRH24159.1"
                     /translation="MEPMREEDWQEKLSPEEYEVLREKGTEAPFSRKYLNHNEEGIYR
                     CKACGNILFTSKQKADSSESDPGLQGWPSFTDPAVAENIGTRPDDSYGMHRTEIYCKK
                     CDGHLGHLFEEKIKGTARKHYCVNSVCLEFEKEGGTAA"
     gene            4197..4736
                     /gene="msrA"
                     /locus_tag="PK109_01035"
     CDS             4197..4736
                     /gene="msrA"
                     /locus_tag="PK109_01035"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRH24160.1"
                     /translation="MKTEVAVFGGGCFWCTEAVFKMLKGVVDVAPGYAGGSKETATYE
                     EVSTGTTKHVEVTRVEYDPAQVSFKDLLTIFFASHDATQVDGQGNDIGPQYRSVVFTT
                     TPAQAEATHAFIDELDASSRDGDPITTTIEPLVEFFPAEDYHKDYYARNASRGYCQVI
                     IAPKLQKVQQQYAELLKTT"
     gene            complement(4785..5258)
                     /locus_tag="PK109_01040"
     CDS             complement(4785..5258)
                     /locus_tag="PK109_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRH24161.1"
                     /translation="MQKITPFLWFDKDAKAAAEFYVSVFGEDSKIRNEQIMPPAPTGD
                     RVEVMNITLRGQDFTLMAAGPYFKFNESVSFVIDCKDQEEVDYFWNALTADGGQESMC
                     GWLKDKYGMSWQVTPRRLIELTTSSDREKAGRATAAMLTMKKIDIAAIEAAYEGK"
     gene            complement(5263..5601)
                     /locus_tag="PK109_01045"
     CDS             complement(5263..5601)
                     /locus_tag="PK109_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24162.1"
                     /translation="MKFIAVYMMPHTGLDEWMKLPEDERKAQENQMKTEWDAWLASHK
                     DAIAETAGVGKPKRVTKEGTTDARNDLMMYSFVEAESADAAAKLFEGHPHFGIPGASI
                     EVMPVNKNIQ"
     gene            complement(5650..5883)
                     /locus_tag="PK109_01050"
     CDS             complement(5650..5883)
                     /locus_tag="PK109_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005260917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRH24163.1"
                     /translation="MKKAVHTLPADFRKAIQASKPAYAVWEDITPLARNEWICWVTSG
                     AKAETRGIRITKAVSKLKGGMRRPCCWAGCPHR"
     gene            complement(5880..6227)
                     /locus_tag="PK109_01055"
     CDS             complement(5880..6227)
                     /locus_tag="PK109_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004470391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRH24164.1"
                     /translation="MKKIETVPAYIATFPKDTQAILKKMRATIKKVAPKAKEKIAYGI
                     PTYTLNGNLVHFGGFKKHVGFYPAPSGIKAFQKELSEYKGAKGSIQFPYDEPLPLALI
                     QKIVRYRIKEDMK"
     gene            6282..6635
                     /locus_tag="PK109_01060"
     CDS             6282..6635
                     /locus_tag="PK109_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRH24165.1"
                     /translation="MEKHRIYTMSFSRVYPLYITKAEKKGRTKKEVDEVIRWLTGYSQ
                     KELSAEIKKNTDFETFFKKAPKLNPSRSLISGVVCGVRVEDIKEPTMREIRYMDKLID
                     ELAKGIPMEKILRKG"
     gene            complement(6644..7066)
                     /locus_tag="PK109_01065"
     CDS             complement(6644..7066)
                     /locus_tag="PK109_01065"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRH24166.1"
                     /translation="MEEAEFDAITEAAWDAMPEKFKVHIENVALLVEDEPSEELRTLE
                     GLGEGETLLGHYHGIPNTARGSEYGVGVTLPDTITLYRLPILDAADEMTHRDGGEFLD
                     NVTTVVRETLWHEIGHYFGLDEHPINEREAEGTNKFES"
     gene            7088..7720
                     /locus_tag="PK109_01070"
     CDS             7088..7720
                     /locus_tag="PK109_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015167142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRH24167.1"
                     /translation="MDIPFLHATLPDIIRSVGYIGVFLIVFIESGVPFGVLIPLPGDT
                     LLFSAGILAASSAFDLIPLIATIVAGAILGDTVGYWFGAKYGPKLFTKEDALFLNKRH
                     LERTERFYEKYGRRAIIFARFLPVFRTIVPIAAGMGSMKYSVFLRYNIAGAFIWGISV
                     TMLGYYLGSVIPDIDKYLLPILFIIIFLSSASVISEVLKARKEQKTAIEG"
     gene            complement(7707..8093)
                     /locus_tag="PK109_01075"
     CDS             complement(7707..8093)
                     /locus_tag="PK109_01075"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HRH24168.1"
                     /translation="MERNTINRATLIGGVVLVVAVFAFGYWLGTRSVPTPEAGTPSTA
                     GVIGDASYQCADEKSMHAVFYENQVTVMLSDGRTFNLAHTISGSGARYASVDDGIVFW
                     EKGQSAFLEEAGTTTYSDCENAPIPL"
     gene            complement(8104..8646)
                     /locus_tag="PK109_01080"
     CDS             complement(8104..8646)
                     /locus_tag="PK109_01080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRH24169.1"
                     /translation="MNILAISGSLRKESFNTHLVRALQARAPEGVTVTIADISDVPLY
                     NQDLEAAFPAPVATLKERIQGADGIVIATPEYGRSVPGVLKNVIDWTSRPYGMSAWTG
                     KPVYVLGATDGAVGTAVAQKEVRSDITTAGGIVLGQPEFYLSKFKEKFSEDGTLTDES
                     TGTHIDKALAAFVAFVQKFQ"
     gene            complement(8760..>9065)
                     /locus_tag="PK109_01085"
     CDS             complement(8760..>9065)
                     /locus_tag="PK109_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRH24170.1"
                     /translation="KFYPWFQAMMEAQDDEGDEGFGDLASIKTLTATIPGIDVAKVEK
                     LMTDKKDEYQKAIAADRVEGGSFGINGTPGTLIGTKVLSGAVGYAQVAALLDAELAK"
BASE COUNT         2226 a         2348 c         2320 g         2171 t
ORIGIN      
        1 ctttggcggc ttcgtggagc atcttcgcgc gtcggttata acctaatccc tgccacatcc
       61 tgagaacgtc agcgagcgac gcttgtgcaa gtgcttctat gtttggaaat cgttcaagcc
      121 aagcaaggta ataggctttg acccgttcta cctgtgtctg ctggagcatc acttccgaca
      181 cgagaatctt gtacggatcc ttactctttc gccatggtaa gtcgtggcgt ccgtgcgttc
      241 ggtagtactt ccacaccgtg gttctgaagg tacgtatgcg gtctactgtc atgcaataaa
      301 gaacccgaat tcagagtcga ggtgcggaca atctattaaa cgggtcatac acactgatcg
      361 tgctcgagga gtgcgagacc ggaagcggcg tagacgagtt tggctatatt tcatagataa
      421 cgtctttacg ttccactacc acgcgccacc cacgcttctg cgcatgctcg tagagtgctt
      481 tgtttggatt aaacgcaagc ggtatctcga cgagctcaag catagggata tcactttcgg
      541 tatctcccac accgatggaa cgctcaagag acagtccagc tttgcgcgcg gcacgctcta
      601 agaccgccgc cttgttacga ataagatcat gatcctcaac gttccccgta aagtttccgg
      661 agggtccggt ctcatagaaa aagccgtaca ctttatcgaa gccgagttca tgcccgaacc
      721 cttcaacgat aaagcggggt gaatgtgaga tagcaagaag gtaataccct ttcgctttta
      781 agtcacgcac catgtcacgc gtgtatcggt agaggcgatg cttcttctca tcaattattt
      841 ctcctgcgat aaaggcgact tcttcatacg agaccccttt aagatttccg cggaacgcgg
      901 tgatcatttt ttcaatatag gtatcgtaat cccctcgcct atcgagccac ttttcttgag
      961 catcaacgta tgcttcgcgc acctctttag gaagaatccc tttatctacc aaccgttcca
     1021 ccagttcaat gaataaggag gagcggaaaa tggtgccgtc gatatcaaag actgcgatgg
     1081 gctgactcat tccctcatgg taccccttcc tggaaaaaac aaaacgccag ctcattgagc
     1141 ggcgtcctgg agagctagct ctagctagca attgtgaaat aaagatctga aagaacgtgg
     1201 ggtcccggcc tgtgtagctg cagcacaaga gttgagcttt agtagggcgt cttgcgatgc
     1261 ttcatgaatg catcacgctc cgccgttcct cagtagccag ccaaatccta gagggaagga
     1321 tcttttcctc acaaggtcca ggttggcacc atccagggtt ggactcctgg cgatgttagg
     1381 acacgcatgc gggctattag ctactccctt gaggtagtct gcttagactc aagtgcgctc
     1441 tagaccggga cccgaagcca ctttaccaga aaggtcaaat taggccaact atttcgattt
     1501 cggccaacgt ttcgccgcag gaggccatat cttagtgagc ggatgggttt cgcagtcgtg
     1561 aggacgtgca ggacagacgt accggccata caggacgaat ccgttattaa cgtacttcca
     1621 gtcttttttg ggaataagag tttcgaggtc ttttgaaatg cgtgcaagat cggtccggtc
     1681 agtcaaatcg aatcgaagcg caaagcgtcg cacgtgcgta tctgtcggaa taccttcgta
     1741 tatgccatga agttcgccga gcactacgtg cgctgtctta tacgcaatac ccggcagccg
     1801 taccagttct gcttctgtct tggggacttt cccatcaaac tcgtctcgta tcatcttggc
     1861 ggcgccgata atcgcctttg ccttattgcg atgaaaggat actgacgcta tctctttatc
     1921 aaaggtcgca tacttcgcat tcgcgaaatc attcaccgtc ttatatttct tccagagcgt
     1981 ctcggtcacc ttattcacct gcttgtccgt agtctgcgct gagagcatca cgctcgcgac
     2041 aagctgaaac ggtgtttgat agttaagttc agtttctgga gatgggtatg cacgttttaa
     2101 gtagcgaacg atgcgtgcag cgcgtgcctt ccgctcagca tgagacgatg ccatacgcta
     2161 gttagccttg aatgcgaaac gataaaagat gacgagaata gccccgagtg cggcgcttga
     2221 gagtgcgaac gcaatgaaaa gaccgagcgc aggaatgacg ccaataaggc cgagtgcgag
     2281 cataccgagc accgctccct tccaggtaac atcagtgcgc ttaagcaggg tgcgcatgag
     2341 cgctgacccc gtgagcactg cagcgtagag gtacgctaaa agaagaagca ggaggtagag
     2401 cgcgccaatg agcgctgcta tcccgatacc tacaaacgaa gcgactaaga ggaggaggag
     2461 aaccggcgtt gctgctatga ccgcaaagcc aagaagtgcg agaaggagga aacgctttgg
     2521 tccgcgcata agtgcttcct cggcaacgcg ctggccaaag accggaaaga gtccagcgac
     2581 aagtcctgca gcaagaatgg cagcgacgag acgcacgata aagaaaatac caataccggc
     2641 gagcgcgaag gtttctgctt cctgtgccgt gggaaggaat gatgaggaac caatatagtt
     2701 tgcaccccca tcaatcaccg cagacgcagg aatactcact tcttgaggtg cgttgtagtc
     2761 aaaggtgccg tggataatgg ttccttcggc aagcgtgaca tggtccgacg cgacgacgcg
     2821 tacgttacca tcgaactctc ctgaaagaag aatgttcgcg ccgtatgccg ttacgggacc
     2881 atgggcgccg gaacgaagct caacggtagc gcccgctgcc cgtatctctc ctgcgctccc
     2941 agaaactatg accgtgccac caaagagggc gagttcccca ctcacatccc ctgtactccg
     3001 tatcgtcccg cctgccgcgc gcacatcacc cgttaccggt gcctgtacga gtacggagcc
     3061 ggcaagcgcc agcacatccc ctgctaccgg agcactaatg cgaagcgagg tagcaagggc
     3121 cataagatcc gccgggagtg cggtgtcgac actaatgtca gtaccagcaa gataggagtt
     3181 tccgccgggt gtttcggaaa caacgagggt ctgatttggt tcttcagccg caagcgcaaa
     3241 gaacggcaaa acagatgaaa ggaggagcgc tgcgagagct cgagcggtca tgcagctatg
     3301 gtatcacgac aggaagttgc ggggcaaaac gcttcaggag gaggtaggac agaggtacgg
     3361 agaggaaggc caggactgcc gaaacaatgc agtagacaca tacggcctga atgaggaaga
     3421 actgaatgaa gaggaagcac acagacgcaa gcgcggacgc cacagatagc gcaaatattc
     3481 ctttatataa aagacgacgt ggcgagaagc agaatgcaag cgcgccaagg atgagaagaa
     3541 cggtgtagaa agcaacgcca aagtacgcga gcgggatgcc catgaactgg gaataggggc
     3601 tctgcgctac cgtattgcag ccatcaagac cagcgccgat gtcacagaca agcgcggtat
     3661 cagtagctgc gtgcgacgcc aggtaccagg agtcggtaat accaaggagg gcgagcagga
     3721 taaggagggc ggccagaagt ctgcgagtca ttggcaggta tagtatcatg cctatatatg
     3781 gaaccaatgc gtgaagagga ctggcaggaa aaactctctc cagaggagta cgaggtcctg
     3841 cgtgaaaaag gaactgaagc acctttttca agaaagtatc taaatcataa cgaagaaggc
     3901 atctaccgtt gcaaagcgtg cggaaacatt ctcttcactt caaaacaaaa agctgattcg
     3961 agtgaaagtg atcccggtct tcaagggtgg ccttctttca ctgatcctgc agttgctgag
     4021 aatataggaa cacgacccga tgattcatac ggcatgcatc gcactgagat ttattgcaaa
     4081 aaatgtgacg gtcatctcgg gcacttattt gaagagaaga taaaaggtac cgcaagaaaa
     4141 cactattgtg tgaactctgt gtgtcttgag tttgaaaaag aaggaggaac tgctgcatga
     4201 aaactgaagt agcggtattt gggggcggct gcttttggtg caccgaagcg gtattcaaaa
     4261 tgctgaaagg cgtggtggac gttgcgcctg ggtacgcggg aggcagtaaa gaaaccgcaa
     4321 cgtacgaaga ggtttctacc ggcaccacta agcatgtaga agtaacgcgc gttgaatacg
     4381 atcctgcgca agtatcgttt aaggacctgc tcaccatatt ctttgcgtcc catgacgcaa
     4441 cccaagtaga cggtcaagga aatgatattg gcccgcagta tcgttctgtg gtgtttacga
     4501 caactcccgc gcaagcagaa gcaacgcatg cgtttattga tgagctcgat gcgtcgagcc
     4561 gcgatggcga ccctatcact acaacgatag aaccgctcgt ggaattcttc cctgctgagg
     4621 actatcacaa ggactactac gcgcggaatg cgtcccgtgg ctactgccag gtgatcatcg
     4681 ctccgaaact gcagaaggtg cagcaacagt acgcagagct tctcaagacc acttaacgaa
     4741 aatggcccct cacggggccg ctttcgttaa cgatgatagg tcatctactt cccttcgtac
     4801 gcggcttcaa tagcggcgat atctatcttc ttcatcgtga gcatagcggc agtagcacgt
     4861 cccgctttct ctctatcgct acttgtggtg agctcgataa ggcgtcgtgg cgttacctgc
     4921 caggacatgc cgtacttatc ctttaaccat ccacacatac tttcctgtcc gccgtctgcc
     4981 gtgagcgcat tccagaagta atcgacttct tcctgatcct tgcagtcgat gacaaatgaa
     5041 acggactcgt tgaacttgaa atacgggccg gcggccatga gcgtgaagtc ctgaccgcgg
     5101 agcgtaatat tcatgacttc cacacggtcc cccgtaggtg ccggtggcat gatctgctca
     5161 ttacgtatct tcgagtcttc accaaagacc gagacgtaga attcagccgc cgcctttgcg
     5221 tccttatcaa accaaagaaa tggcgtgatc ttttgcatat atctactgaa tattcttatt
     5281 caccggcatc acctcaatag aggcgcccgg gataccgaag tgcgggtgac cttcgaagag
     5341 ttttgctgcg gcgtctgcag attctgcttc gacaaaggag tacatcatga ggtcgttgcg
     5401 cgcatccgtg gttccctctt tggtgacgcg ctttggcttc ccaacacctg cagtttcagc
     5461 gatagcatcc ttgtgtgacg ctaaccacgc atcccattcg gtcttcatct gattctcctg
     5521 ggctttgcgc tcatcttcag gaagcttcat ccattcatca aggccggtgt gcggcatcat
     5581 atatacggca ataaatttca taggtagtag ttatttctta atgtgggcag tatagcaatt
     5641 ttgtatgagt cagcgatgag ggcatccggc ccagcagcac gggcgtcgca tcccgccttt
     5701 aagctttgat accgctttcg taatacgaat accccgcgtc tctgcttttg ctcctgaggt
     5761 cacccagcag atccactcgt tacgcgccaa gggagtgata tcttcccata ccgcgtaagc
     5821 gggcttagac gcttgtatcg cctttctaaa gtcagcaggg agcgtgtgta ctgctttttt
     5881 catttcatat cttccttaat tctatagcgc acaatcttct gaatgagcgc aagcgggagt
     5941 ggttcgtcat acgggaactg aattgaacct ttcgctccct tgtactctga aagttctttt
     6001 tgaaatgcct ttatgcctga gggagcagga tagaagccga catgcttttt gaacccaccg
     6061 aagtggacga gattcccatt taaggtatag gtcggtattc catacgctat cttctctttt
     6121 gctttgggcg ctactttctt aatagtagcg cgcatctttt tgagtatggc ctgcgtgtcc
     6181 ttagggaagg tggcgatata tgcaggcacg gtttctatct tcttcatgac ttcaagtata
     6241 tatcgggttg ccttctgctc caccagtgct acaaagaggc tatggaaaag caccgcatct
     6301 acactatgag cttctcgcgg gtataccctc tgtatatcac taaggcagaa aagaagggac
     6361 gtaccaaaaa ggaagtggat gaggtgatac gctggctcac cggatacagc cagaaagaac
     6421 ttagtgcaga aataaaaaag aacacggact ttgagacgtt ctttaaaaag gcaccaaagc
     6481 ttaacccctc acgctcactc attagcggcg tggtgtgcgg cgtaagagtt gaagatatta
     6541 aggagcctac gatgcgggag attcgctata tggataagct tattgatgag ttggcgaaag
     6601 gaataccgat ggagaagatt ttgcgtaagg ggtagctgac gggctaggat tcgaacttat
     6661 tcgtcccctc tgcttccctc tcgttgatag gatgctcatc aagaccgaag tagtgcccta
     6721 tctcatgcca taacgtctct cggacgacgg tggtcacatt atcaagaaac tcaccaccgt
     6781 cccgatgcgt catctcatct gccgcgtcca gtatggggag acggtacaga gtgatggtgt
     6841 cgggaagcgt gacccccacg ccatactcgc ttccgcgtgc ggtgttgggt atgccgtgat
     6901 agtggccgag tagtgtctct ccttcaccga gtccttcaag tgtgcgcagt tcttcacttg
     6961 gctcgtcttc caccagaagc gctacgttct ctatatgcac cttgaacttc tcaggcatag
     7021 cgtcccacgc cgcttcggta atagcatcaa attctgcttc ctccatgcgg gctagtatac
     7081 tctccagatg gatatccctt tccttcacgc caccctcccg gacatcattc gctccgtggg
     7141 ctacatcggc gtattcctta tcgtgttcat tgagtcgggt gtaccgtttg gcgttcttat
     7201 tccactcccg ggagacacgc ttctattctc tgccggcata cttgccgcgt cctcagcctt
     7261 tgatctcatt cccttgattg cgaccatagt agcgggcgcc atacttggtg acacggttgg
     7321 atactggttc ggtgctaaat acggaccaaa gctcttcacc aaagaagatg cgctcttttt
     7381 aaataagcgg cacttggaac gcacggagcg cttctatgaa aagtacggac gccgcgccat
     7441 tattttcgcg cgcttccttc cagtatttcg tactatcgtc ccaatagccg ccggcatggg
     7501 gagcatgaag tacagcgtgt tccttcgcta taacattgcg ggtgcgttta tctggggcat
     7561 ctcagtcacg atgctcgggt actacctcgg cagcgtcatc cctgatattg ataaatatct
     7621 tctccccata ctctttatca tcatctttct ctcaagcgct tctgtgatta gtgaagtgct
     7681 aaaagcgagg aaggagcaga aaaccgctat agagggatag gcgcgttttc gcagtcgcta
     7741 taggtagtag tacctgcttc ctctagaaat gcgctctgtc ctttctccca gaatacgatg
     7801 ccgtcgtcta cagaggcgta gcgtgcccca gatcctgaga tggtgtgcgc caagttgaag
     7861 gtacgcccat cagagagcat gacggttacc tgattttcat agaagacggc gtgcatagat
     7921 ttttcatcag cgcactgata gctcgcgtct ccaataacac ctgcagtact tggtgtaccc
     7981 gcttctggtg tcgggacaga acgcgtcccg agccaatacc caaatgcaaa cacagcgact
     8041 acgaggacta ctccccctat aagcgttgcc cggttgatgg tattcctttc cattaccgat
     8101 agtttactgg aacttctgta caaatgcgac gaaggcagca agtgctttat caatgtgggt
     8161 gccggtactc tcatcagtaa gagttccgtc ctcgctaaac ttctctttga acttcgagag
     8221 gtagaactcc ggctgtccaa ggacgatgcc gcctgcagta gtgatatctg aacgcacttc
     8281 tttttgtgca accgcagtcc ccactgcccc atccgtggca ccgagcacat acaccggctt
     8341 acccgtccac gcactcatgc cataaggccg actcgtccaa tcaatcacat ttttaagtac
     8401 gccgggaacg gagcgaccat actcgggcgt tgcaatcacg ataccgtcgg caccttgtat
     8461 gcgttctttc aatgtcgcta cgggtgcagg aaacgccgct tcaagatcct ggttatacaa
     8521 aggaacgtca ctaatatcag caatagtcac cgttactccc tccggcgcgc gtgcctggag
     8581 agcgcgtacg agatgcgtgt taaatgattc tttacgaagg cttccagata tagcaagaat
     8641 gttcatagtg gtgcggtgat gggtaggacc tctaccctac cgcgcttctg accttcgaag
     8701 aggtggggat aacgaaacac cgcccaagag agcggtgttt cagcacattt caactgcgtt
     8761 tatttcgcga gctcagcgtc aagaagtgct gctacctgtg cgtagccaac cgctccagag
     8821 aggaccttgg taccgatgag agtaccaggg gtgccgttaa tgccgaagct gccaccttca
     8881 acgcgatcag cggcgatagc cttctgatac tcgtccttct tgtcggtcat aagcttctct
     8941 accttcgcga catcaatgcc aggaatggta gcggtcaagg tcttgataga agcgagatca
     9001 ccgaagccct catcgccttc atcatcctgt gcttccatca ttgcctggaa ccaaggatag
     9061 aactt
//
LOCUS       DAORIN010000014         8539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1456_length_8539_cov_5.217350, whole genome shotgun sequence.
ACCESSION   DAORIN010000014 DAORIN010000000
VERSION     DAORIN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1456_length_8539_cov_5.217350"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..633)
                     /locus_tag="PK109_01090"
     CDS             complement(76..633)
                     /locus_tag="PK109_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRH24171.1"
                     /translation="MTTHRPMRAYTLLELIVSIGIFSIVMLTATGAYIKLIDLDRQAR
                     GTNDVVSNLSFAVDSMTRAVRTGKDYKCNITGTNCTNTSSPGTSLRFTDEQGRTVTYE
                     LESGQIKATIVPLTGSTISAKLTDPRITVSTLNFYVRGVSVESGTKIQPQVTFIIAGS
                     MVTGPGKTVNFSIEGGATQRVLELP"
     gene            complement(630..1331)
                     /locus_tag="PK109_01095"
     CDS             complement(630..1331)
                     /locus_tag="PK109_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRH24172.1"
                     /translation="MESSFFRTRALTKHPRTARGFTLVEMLVVLTIITVITMIALLGQ
                     GDFNRSILLTDTAYTVALSLREMQTLGLSSRKFTNIQNAGYGGYFTAASPANKQFILF
                     ADTTSAGSVPSNCNVGPTVTPTPESKPGDCIYTSGSDGIVQTYTFNRGFTISEVCGTD
                     SGIRKCSSTGAMTALSIVFLRSATESMMVGKNSSNVWVPITKAEIYINSGNGASRAIC
                     VSQVGQISVAFTTCP"
     gene            complement(1298..2059)
                     /locus_tag="PK109_01100"
     CDS             complement(1298..2059)
                     /locus_tag="PK109_01100"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRH24173.1"
                     /translation="MELLTAFFFGLFGAIAGSFAGVIAERMNTGQSFLKGRSRCNSCA
                     RTLGVPDLVPVLSWIFSQGRCRTCGAGVPGLYALLELSLGALFALAFLKVGFVLALAP
                     LLIALFLLAIIVVYDLRHTIVPPSLSFLLFLSCVTYALLSAYDMHAFGLALLVAGAIA
                     LAFFLLHVCSGGRAMGLGDAPIALSLSLLAAGNAISGILFAFWIGALYGIAVLVMRQG
                     GPKMGIEVPFVPFLAAGALLAYFTGWNPLFSALAL"
     gene            complement(2144..2605)
                     /locus_tag="PK109_01105"
     CDS             complement(2144..2605)
                     /locus_tag="PK109_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRH24174.1"
                     /translation="MKSVLSLGHVRRGFTLIELLVVIAIIGILSSVVLASLNTARSKG
                     TDAKIQSEIRSIGVNAEIYYDNNGNSYGSATTTTCTTGMFASTNNPNIAQIVTDLNAA
                     VGTAADATCIAGPSGWAVQHQLQSQTGKFCMDSTGKVSTSTTAISATQLCS"
     gene            complement(2703..3911)
                     /locus_tag="PK109_01110"
     CDS             complement(2703..3911)
                     /locus_tag="PK109_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRH24175.1"
                     /translation="MLFNYKALDKDGHERGGTVEALSMDVAVSTLQRRGLIVSSIDPA
                     STKSVLSLEFGFLKSVSNKDVVILSRQVATLFEAQVSALRVFRLLAAEVDNAYLAEIL
                     TQVADDLQGGSPISKALARHPKVFSSFYVNMVRSGEESGKLSEIFGYLADYLDRTYEV
                     MSKAQNALIYPAFVVATFVVVMTLMLTLVIPNVSKILIDSGQEIPLYTQIVIGLSNII
                     VNYGAFVLILLVVGGFFIYRSMQNPAGRYFFDNLKLETPYIGDLYEKLYLSRISDNFA
                     TMLVSGVPVVESVEITSSVVGSAVYERILAQVGEDVKGGSSISDALSKHEQIPGIMTA
                     MVKVGEETGELGNILNTLAKFYRREVSTSVDTLVDLIEPLMIVFLGLGVGTLLAAVLI
                     PIYNLAGSIS"
     gene            complement(3962..4405)
                     /locus_tag="PK109_01115"
     CDS             complement(3962..4405)
                     /locus_tag="PK109_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24176.1"
                     /translation="MSESGERPFVPATTGERERKPSLNEQETLALLAQKGVAGLGAMK
                     PHTPFLYVLEEHTRLDTATPDVKASIARVEYPERFEQVYPGRFRILIKKGQIDYYAIH
                     RDTGQPFHVGTGQPRERNKQGIIYLANTSNMFEAADHLFPKAKKP"
     gene            complement(4411..5478)
                     /locus_tag="PK109_01120"
     CDS             complement(4411..5478)
                     /locus_tag="PK109_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRH24177.1"
                     /translation="MTAAGKDTISALLDTVIAEGGSDLHLSPGSPPIIRVAGALVPLL
                     QKEVLTGADTEAMLKTLLPPDRWDTFTKEQSVDFSHEHTDKNRFRVNGFRTGNSIAIA
                     MRLIPKEIRTFATLNLPPILEVFTQRQQGFFLVVGPVGQGKSTTLAAMIDRINETRAE
                     HIVTIEDPVEYLFEKKKSLIHQREVHIDTPDFHQALNAAFREDVDVIMVGEMRNYETI
                     SSAVTAAETGHLVLSTLHTNNAAQTIDRIIDMFPSTQQDQVRVQLAGSLAGIFSQRLV
                     PRIAGGLVPAYELLINNSAISNLIREGRTHEIGTVVQTSSQEGMIDMDRSLAELVRKG
                     EVTVEDAYERASDQKTFERYL"
     gene            complement(5475..5858)
                     /locus_tag="PK109_01125"
     CDS             complement(5475..5858)
                     /locus_tag="PK109_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRH24178.1"
                     /translation="MANHRIYLVDDDRFLLDMYAIKFKNAGHEVTAFQSGDEVLAALK
                     KDPAPDAVLLDVVMPGMTGFETLETIRRENIAPTTKFIILSNQGQDTDLDKAKELNAS
                     GYIIKASAIPSEVYAETIKIIEKPA"
     gene            complement(5867..7642)
                     /locus_tag="PK109_01130"
     CDS             complement(5867..7642)
                     /locus_tag="PK109_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRH24179.1"
                     /translation="MNILPLLVKKGTLKAGDVSAVENEARKDKISVEAVLNKRGITAE
                     DILSAASDHYNIPGRVIPTGPIGHAGLEYIPEESARHYRLVPIGVADGALEVGITDPD
                     DIEALDALQFISTKAGMPYKLFLITESDFARVIDEYKNLTGEVGEALSELETSLDDVL
                     TKRKEEEEEGRAMTEDRIKADAPVTKIVATMLRYAVEGGASDIHVEPTPEKTRVRFRM
                     DGELHTSLELPAKVHPAVVARIKILCQMRLDEKRKPQDGRFSATVEGRRIDFRVSTFP
                     TEFGEKVVMRILDRAKSVATLDTVGLTKEDLATIREIIRVPYGIILMSGPTGSGKSTT
                     LFAMLSELDRETQNVVSIEDPVEYEIPQVAQSQVHPEIGYTFASGLRSILRQDPDVIM
                     VGEIRDKETAQLAIQAALTGHLVFSTIHTNTAAGVIPRLVDMGVDPYLIAPTLVAAIG
                     QRLVRKLCPGAGEEMPVTDSIKLLLEKDFADLPAEHKGRIPKFEKLYRAAPTPDCPNG
                     TRGRVGVFEILRMTKSLEHVVLTDPVEEKVYAAARAEGMLTMREDGIIKALNGEIPFE
                     EVNTLGGALLADEEDNAPEKEPEAT"
     gene            complement(7700..7996)
                     /locus_tag="PK109_01135"
     CDS             complement(7700..7996)
                     /locus_tag="PK109_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24180.1"
                     /translation="MHMSKTTGIAIALGVLLLIAAAYLMFRPSNLAGVAATGAPSSEA
                     ELTFITLTAKIDPVELDTSILTDPRFVGLQDIRTAVIPEPSGRTDPFAPLPGVR"
     gene            complement(8000..>8539)
                     /locus_tag="PK109_01140"
     CDS             complement(8000..>8539)
                     /locus_tag="PK109_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24181.1"
                     /translation="RILPVLLILIAIGLFFGYVNPTYTGAVAALRTEIQGYDAALAAA
                     DKFAEKERALLAERNSIPAEGLARIESFLPDGVDNVQLILDLNSLASRSGLTLSNFDV
                     GTPAETSGGDRLSLASESPVESLDISVSATGSYAALKTFLLGAEWSLRPLDLTDISIS
                     ESKTGVYTYDLTFRIYWLR"
BASE COUNT         2049 a         2166 c         2353 g         1971 t
ORIGIN      
        1 ctacggcaag caccgtaatg cccaccatcg tttctacgag agtgaatcct ttctgtggtt
       61 tcataggagt gtaggtcatg gtagttcaag gacacgctgg gtggcacctc cttctattga
      121 gaagttcacc gtcttccccg gacccgtcac catgctgccc gcgataatga acgttacttg
      181 tggctgaatc ttcgttccag actcaacgga cacaccgcgc acatagaaat taagggtgct
      241 cacggtgata cggggatcgg taagttttgc tgagatggtg ctcccggtaa gtggaacgat
      301 agttgcctta atctgtccgc tttcaagttc gtacgtaacc gtgcgtccct gctcatccgt
      361 aaagcgaagt gacgtaccag gactactcgt gttcgtgcag ttcgttcccg tgatattgca
      421 cttatagtcc ttacctgtgc gtaccgcgcg cgtcatgcta tctaccgcaa aagaaaggtt
      481 tgataccaca tcgttcgtgc cgcgtgcctg tctatcaaga tcaatgagct ttatgtacgc
      541 ccccgttgca gtaagcatca cgatactgaa gataccgata gagacaatga gctcaaggag
      601 ggtgtacgca cgcatgggtc ggtgcgtggt catgggcagg tagtgaaggc aacggatatc
      661 tgaccgacct gagacacgca gatagcacga gaggcgccgt taccggagtt tatgtatatc
      721 tccgctttgg ttatcggtac ccagacgtta ctactgttct tccctaccat catgctctcc
      781 gtagccgagc gcaagaagac gatggagagt gccgtcatcg cgcccgttga cgagcactta
      841 cgaattccac tgtcggtacc gcacacttcg cttatggtaa atccgcggtt gaaagtatag
      901 gtctgcacga tgccgtctga gcctgaggtg tagatacaat cacccggttt tgattcgggt
      961 gttggtgtaa cggtaggtcc tacattgcag ttggaaggaa cggagcctgc tgacgtggta
     1021 tctgcaaaaa gaatgaactg cttattcgcc ggcgacgccg cggtgaaata gccgccgtaa
     1081 ccagcgtttt gaatgttcgt gaatttccta ctcgaaagac ctaaggtctg catctcacgg
     1141 agtgaaagcg ctaccgtgta cgccgtatca gtcagaagga tagaacgatt gaaatctccc
     1201 tgccccaaaa gggcaatcat ggtgatgact gtgatgatag tgagcaccac gagcatctca
     1261 acgagcgtaa agccgcgagc cgtgcgtggg tgcttcgtta aagcgcgagt gcggaaaaaa
     1321 gaggattcca tccggtgaag tatgcgagga gtgcgcctgc cgcaagaaac ggcacgaacg
     1381 gcacctcaat tcccatttta ggccctccct ggcgcatcac caacacagcg atgccgtata
     1441 acgctcctat ccagaatgcg aagagtatgc cggagatggc gtttcctgcc gcaagtaagg
     1501 aaagggagag tgctatcggc gcgtcaccta gtcccatcgc tctaccgccg gaacagacat
     1561 gcagcaaaaa gaacgctaat gctattgcgc ctgcaaccag aagcgccaac ccaaatgcat
     1621 gcatatcata ggcagacagg agcgcatacg tgacacacga gagaaacaga agaaacgaga
     1681 ggctcggcgg cacgatggtg tgccggaggt catacacgac gatgatagcg agcaggaaaa
     1741 gcgcaatgag aagcggtgca agcgccagca cgaatcctac ctttagaaac gcgagtgcaa
     1801 acaatgcacc aagagaaagc tcaagaagcg catagagccc gggcacccct gccccgcagg
     1861 tacggcaacg gccttgagaa aatatccagg agagtaccgg taccagatca gggacaccta
     1921 aggtgcgcgc acatgagtta cagcgcgagc gacctttcaa aaaggactga cccgtgttca
     1981 tgcgctcagc gataacgccc gcgaagcttc ccgctatagc accaaagagt ccgaagaaaa
     2041 atgcggtgag tagttccatc ccgctatcat aacgcgtcga tggatatata aaacgaaaac
     2101 gcgcgccata gtggcgcgca tcctcgttac cctgccttac ggattaggag cagagctgcg
     2161 ttgcgctgat ggccgtcgtt gaggttgaga cctttcccgt cgagtccatg cagaacttgc
     2221 ccgtctgtga ctggagctgg tgctgaactg cccaaccgga aggaccggcg atgcaggtag
     2281 cgtcagctgc cgtacctact gctgcgttca ggtcagtgac gatctgagcg atgtttgggt
     2341 tgttcgtgga agcgaacata ccggtggtgc aggtggtggt cgttgcagaa ccgtagctgt
     2401 tgccgttgtt gtcgtagtag atctcagcgt ttacgccgat agagcggatt tcgctctgga
     2461 tctttgcgtc agtacccttc gagcgcgccg tgttgaggga cgcgagcacg acggatgaga
     2521 gaatgccgat aatagcgatg acgacgagga gctcgatgag cgtaaaaccg cgtcggacat
     2581 ggccgaggga aagtacagat ttcatagtga gggtgagtga taaggcgggt aaagtatccg
     2641 ctccccgtat tgtacgagag attacgccac gaattggcac tcgtgtggat aaccacacgt
     2701 aattatgaaa tagaaccagc gaggttgtag ataggaatca acactgcagc aaggagtgta
     2761 ccgacgccaa gaccgaggaa tacaatcatc agtggctcaa taaggtcgac aagagtatca
     2821 actgaagtag atacttcgcg acgatagaac ttcgcaagtg tattcaagat gttgccaagc
     2881 tctcccgtct cctctcccac cttcaccatc gcggtcatga tgcctgggat ctgctcatgc
     2941 ttagaaagtg cgtctgaaat ggaagagcct ccctttacgt cctcccctac ctgcgccagg
     3001 atgcgttcgt acacggcgct tcccactacc gatgatgtga tttcaacgga ttcaacgaca
     3061 ggaactcccg agacaagcat ggtcgcgaag ttgtctgaga tgcgcgagag gtagagcttc
     3121 tcgtagaggt cgccgatgta aggggtctca agcttcaagt tatcaaagaa gtaacggccc
     3181 gcagggttct gcatggagcg atagataaag aacccaccca cgacgagaag gatgagtaca
     3241 aatgcgccgt aattcacgat gatattggag agcccaataa caatctgggt atagagcggt
     3301 atttcctgac ctgaatcgat aagaatcttg gagacgtttg gaataacgag cgtaagcatg
     3361 agtgtcatca ccaccacgaa ggtagccaca acgaatgctg ggtagataag cgcgttctgc
     3421 gccttactca tcacttcata ggtacgatca aggtagtcag caaggtaccc gaatatttca
     3481 gaaagctttc cggactcttc tcccgaacgc accatgttca catagaaaga ggagaacacc
     3541 ttcggatggc gcgcgagcgc tttgcttatg gggctccctc cctgaagatc gtctgccacc
     3601 tgcgtaagaa tttcagcgag ataggcgtta tctacttctg ctgcaagaag acggaacaca
     3661 cgaagtgctg acacttgcgc ctcgaagagt gttgccacct ggcgcgaaag aataacgacg
     3721 tccttgttgg atacagactt caagaatccg aattcaagtg agagcactga cttagtgctt
     3781 gctggatcaa tcgaagaaac gatgagacca cgacgctgaa gcgtggagac cgcaacgtcc
     3841 atagaaagcg cttcaacggt accgccgcgc tcgtgtccgt ccttatcaag tgccttatag
     3901 ttgaacaaca tgtacatcta gtatcgcacg gtcctaccgc tgcgacgtca tagagcgggg
     3961 attatggttt tttcgcctta ggaaagaggt gatcagcagc ttcgaacatg ttgctggtat
     4021 ttgctaggta tatgatgcct tgtttgttcc gttctctcgg ttgacctgtt cctacatgaa
     4081 aaggctggcc ggtatcacga tgtatcgcgt agtaatctat ctgacctttc ttgataagaa
     4141 tacgaaagcg acctgggtat acctgttcga accgctctgg atactcgacg cgagcaatac
     4201 ttgctttgac atcaggagtg gcagtgtcca aacgcgtatg ttcttcaaga acgtaaagga
     4261 aaggcgtatg cggtttcatc gctcccaagc cagccacccc tttctgagcg agcagggcga
     4321 gcgtttcttg ctcgtttaaa ctcggttttc gctcccgttc ccccgtggta gctggcacga
     4381 acggacgctc accactctca ctcatagaga ttagagatat cgctcgaaag ttttctgatc
     4441 agatgcgcgt tcatacgcat cctcaacggt cacctcaccc ttacgcacca attccgcgag
     4501 tgagcgatcc atatcaatca tgccctcctg gcttgaggtc tgtaccaccg taccaatctc
     4561 atgtgtgcgt ccttcacgaa taagattgga gatagcgctg ttgttgatga gaagctcata
     4621 ggcagggaca aggcctccgg caatacgcgg cacgaggcgc tgggagaaga taccggcaag
     4681 ggagcctgca agctggacgc gcacttgatc ctgttgggta gacgggaaca tgtcgatgat
     4741 gcggtctatg gtctgtgcag cgttattggt gtggagcgta gaaagcacga ggtgaccggt
     4801 ttcagcggcg gtaacggctg aggagatggt ctcgtagtta cgcatctctc ccaccatgat
     4861 gacgtccacg tcttcgcgga aggcggcgtt cagtgcctga tggaagtcag gggtatcaat
     4921 atgcacttca cgctgatgga tgagtgactt cttcttttca aagaggtact cgacggggtc
     4981 ttctatcgtt acgatgtgct cagcacgcgt ctcgttaatg cgatcaatca tcgcggcaag
     5041 cgtggtcgac ttaccctgcc ctactggacc caccacgagg aagaatcctt gctggcgctg
     5101 cgtgaacact tcaagaatcg gtgggaggtt cagagttgca aaggtacgaa tttctttcgg
     5161 gatgagacgc atcgcgatag caatactgtt tcccgtacgg aatccgttca cgcggaatcg
     5221 gttcttgtcc gtgtgctcgt gcgagaagtc tacggactgt tccttcgtga aggtgtccca
     5281 gcggtctggc ggaagaagcg tcttcaacat cgcttccgtg tctgctcccg tgagtacttc
     5341 tttttgaaga agcggaacga gtgcgccggc gacgcgaata atcggaggac ttcccggaga
     5401 aagatggagg tcagacccgc cttcagcgat gacggtatca aggagcgcac ttatcgtgtc
     5461 tttacctgca gcggtcatgc tggcttttcg attatcttaa tagtttctgc atacacttct
     5521 gacgggatag ctgatgcttt gatgatgtag cctgaggcat tcaattcttt tgccttatcc
     5581 aaatcggtgt cctgtccctg gtttgagaga atgatgaact tggtcgtagg cgcaatgttc
     5641 tcgcgacgta tggtctccag ggtttcaaac cctgtcatgc ctggcatcac cacatctaga
     5701 agaacggcgt caggagcggg atctttcttg agtgctgcca atacttcgtc ccctgactgg
     5761 aaggcagtaa cctcgtggcc agcgttctta aacttaatcg cgtacatgtc gagaaggaag
     5821 cggtcatcgt cgacaagata tatgcggtgg tttgccatag aaaatgttat gtggcttcag
     5881 gttctttttc tggtgcgttg tcttcctcgt ctgcaagcaa tgccccacca agcgtattca
     5941 cttcctcgaa ggggatctcg ccgttgagtg ccttaataat accatcctca cgcatggtga
     6001 gcatgccttc ggcgcgtgcg gctgcataga ctttctcctc caccgggtcc gtgagcacga
     6061 cgtgctcgag ggacttagtc atgcgtagta tctcgaagac accgacgcgg cctcgggtac
     6121 cgttcggaca atcaggggtg ggtgccgcac ggtagagctt ctcaaactta gggatacgtc
     6181 ctttgtgttc tgccggtaaa tcagcgaaat ccttctcaag aagaagctta atagaatcag
     6241 tgactggcat ctcctcccct gctcctgggc agagctttcg cacaagacgc tggccgatag
     6301 cggcgaccag ggtcggtgcg atgaggtacg gatcaacgcc catatctacc aagcgcggaa
     6361 tgacgcctgc ggcggtattc gtgtggatgg tggagaacac taagtgaccc gtgagtgccg
     6421 cttgaatagc aagctgcgcg gtctccttgt cgcggatttc tcccaccata atgacgtccg
     6481 ggtcctgacg aagtatggag cggagtcctg acgcgaaggt gtagccaatc tcaggatgta
     6541 cctggctttg cgccacttgc ggtatctcat actccactgg gtcttcaata gacacgacgt
     6601 tctgcgtttc gcgatcaagc tcagagagca tcgcgaacag tgtcgttgat ttacccgaac
     6661 cagtaggacc cgacataagg ataatgccgt acgggacgcg aatgatctca cgaatggtcg
     6721 caaggtcttc tttggtgaga cccacagtat caagggtcgc aacagatttt gcacgatcca
     6781 aaatacgcat caccaccttc tcaccgaact ctgttgggaa ggtagaaacg cggaagtcga
     6841 tgcgacggcc ttctacggta gcggagaaac gtccgtcctg cggttttcgt ttctcatcaa
     6901 ggcgcatctg acagagaatc ttgatgcgcg caacgacagc aggatgaacc ttcgctggaa
     6961 gctcaagaga ggtgtggagt tcgccgtcca tacggaagcg gacacgggtt ttctcagggg
     7021 ttggctctac gtgaatgtcg gacgctcccc cttctacggc gtagcgaagc attgtcgcta
     7081 caatcttggt gaccggcgca tcagccttga tgcgatcttc ggtcatcgcc cttccctcct
     7141 cctcttcttc ctttcgtttc gtaagcacat catcaagcga ggtctcaagc tcagagagcg
     7201 cttctcctac ttctccggtg aggttcttgt actcatcaat aacgcgtgcg aaatctgatt
     7261 ccgtaatcaa gaagagcttg tacggcatcc ccgccttagt ggagatgaac tggagcgcat
     7321 caagcgcctc tatgtcgtcg gggtcagtaa tacctacctc aagcgcgccg tcagcaacgc
     7381 caatagggac gaggcggtaa tggcgagcag actcttccgg tatgtactca agtcctgcgt
     7441 gacctatagg ccccgttggg atgacgcggc ctgggatgtt gtagtggtcg gacgcggcgc
     7501 tcaaaatatc ttctgcagtg atgccacgct tgttgagcac tgcttctaca gagattttat
     7561 ctttacgcgc ctcgttctca accgcggata cgtcaccggc tttaagcgtg ccctttttta
     7621 cgagaagcgg aagtatgttc atgacctacg ttgtagtatc gcatacccca ctgccatagc
     7681 ggttgcgtct gactgtcaat taccgaacgc caggaagtgg agcaaacgga tcggtgcgac
     7741 ctgaaggttc aggaataact gcggtgcgaa tatcctgcag accaacgaat cgaggatcgg
     7801 tcagtataga ggtatcaagt tctactggat caatctttgc ggtaagcgta ataaaggtaa
     7861 gctccgcttc gcttgaaggg gcgcccgttg ccgctacccc ggcgaggttc gagggacgga
     7921 acatgaggta cgccgcagcg atgaggagga gaactccgag tgcgatggcg ataccggtgg
     7981 ttttagacat gtgcatatat cagcggagcc agtaaatacg gaacgtcagg tcgtaggtgt
     8041 agacgcctgt cttcgattca ctgatggaaa tatcggtgag atcaagcggg cgcagactcc
     8101 actctgctcc aagaaggaag gtcttgagag cagcgtagct tccggtagca cttacggaaa
     8161 tatcaaggga ttccaccgga ctctctgacg caagggagag acggtctccc ccacttgttt
     8221 ctgcaggggt accgacatcg aaatttgaga gcgtaagtcc tgagcgtgac gcgagcgagt
     8281 taaggtcaag aatgagctgc acgttatcaa cgccgtccgg aaggaatgac tcgatgcggg
     8341 caagtccttc ggcaggtata gagtttcgtt ctgccaaaag cgctctctct ttctctgcaa
     8401 acttatcagc cgctgcaagt gctgcgtcgt acccttgtat ctcagtgcga agcgctgcta
     8461 ccgcgcctgt gtatgttgga tttacgtagc caaagaaaag gccgatggcg atgagtatga
     8521 ggaggacggg aaggatgcg
//
LOCUS       DAORIN010000015         8503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1465_length_8503_cov_4.308002, whole genome shotgun sequence.
ACCESSION   DAORIN010000015 DAORIN010000000
VERSION     DAORIN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_8503_cov_4.308002"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK109_01145"
     CDS             complement(<1..282)
                     /locus_tag="PK109_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRH24182.1"
                     /translation="MATPSKSALRARRHSRIRARITGTAERPRLAVFRSNKFISAQVI
                     DDAAGKTLVSAHGREFPGSLGKQAGAVGKAIAERATKAGVTAIVFDRGGY"
     gene            complement(289..822)
                     /gene="rplF"
                     /locus_tag="PK109_01150"
     CDS             complement(289..822)
                     /gene="rplF"
                     /locus_tag="PK109_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRH24183.1"
                     /translation="MSRLAKKPIAIPGKVQVTSSDGMLTVKGSKETLTKAVHSSISIV
                     VGPEGVQITPKNTSRLAHALTGTFAAHVKAMMQGVETPFKKTLILTGVGYKVELKGKD
                     LVLSVGFSHQVTLPIPEGLTATVTKNNIVIEGADKQRVGQFAAEIRAVKPPEPYLGKG
                     IRYDDEVILRKQGKKAV"
     gene            complement(830..1222)
                     /gene="rpsH"
                     /locus_tag="PK109_01155"
     CDS             complement(830..1222)
                     /gene="rpsH"
                     /locus_tag="PK109_01155"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRH24184.1"
                     /translation="MVSDRVGDFIIRLKNAGAIGTASISLPHSNHLEAIAKKLKQLGF
                     VADVSVSKKDHKMLEVALAYTERGEHKINGVKRISKPGRRLYAPAKLAHSVKNGLGAR
                     IMSTPKGVLSDAEARKVRAGGETLFEIW"
     gene            complement(1229..1498)
                     /gene="rpsN"
                     /locus_tag="PK109_01160"
     CDS             complement(1229..1498)
                     /gene="rpsN"
                     /locus_tag="PK109_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRH24185.1"
                     /translation="MAKTSQLARNRKRVKLVAKYAAKRAELKAAGDYEGLQKLPKNSS
                     PIRVKNRCSISGRGRGYLRAFGLSRIQFRELARDGKIPGVKKASW"
     gene            complement(1689..2108)
                     /locus_tag="PK109_01165"
     CDS             complement(1689..2108)
                     /locus_tag="PK109_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24186.1"
                     /translation="MKKITTLIFVLVAIPTGSYAQEEAPVTDYASTVPFYAGDSEVPQ
                     NVLVVIGRRSFRESRHDEWRQRNGVQRSYENDGGERQYVYTRRLFGTDEGVSVGTRLT
                     GTDANGITPRGVPNDNGTRGAFRGALVFPTPLFGTVE"
     assembly_gap    2148..2247
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2248..2636)
                     /gene="rplE"
                     /locus_tag="PK109_01170"
     CDS             complement(<2248..2636)
                     /gene="rplE"
                     /locus_tag="PK109_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRH24187.1"
                     /translation="MMLSKQKQQNAYEALAESQGYTNKFQAPRILKVVVSSGTGKAKD
                     KARNKLVDEKLARITGQKPSARPAKTSIAQFKMREGDIIGYQVTLRGARMYEFLDKLI
                     HIALPRTRDFRGIAPTAVDQMGNLTIG"
     gene            complement(2648..2956)
                     /gene="rplX"
                     /locus_tag="PK109_01175"
     CDS             complement(2648..2956)
                     /gene="rplX"
                     /locus_tag="PK109_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRH24188.1"
                     /translation="MKIKKGDTVMLMSGKDKGKTGVVLVAIPSTGRVVVDGIAMVKRH
                     LRRTGRKQSGAIVERPASVNASNVMILDPETKKPTRIGREKQNGKTVRMAKKSKTVIK
                     "
     gene            complement(3049..3426)
                     /gene="rplN"
                     /locus_tag="PK109_01180"
     CDS             complement(3049..3426)
                     /gene="rplN"
                     /locus_tag="PK109_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRH24189.1"
                     /translation="MIQDRSIVTVADNTGAKVGRVFKVLGGSKRRYAGLGDVVVISIQ
                     TAEPRKATKKKDVCKAVVVRQTKAFRRANGSYIRFDDNAVVIIEKQGKEMGPKGNRVF
                     GPVARELTERGWQNIASLAPEIV"
     gene            complement(3429..3671)
                     /gene="rpsQ"
                     /locus_tag="PK109_01185"
     CDS             complement(3429..3671)
                     /gene="rpsQ"
                     /locus_tag="PK109_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRH24190.1"
                     /translation="MEPTISKPQSFKGVVVKTAMKDTATVAVSRYVKHPKYKKYQTRT
                     KKYLAHDPGNTVQVGDRVTIVACRPISKLKRFKIAN"
     gene            complement(3680..3874)
                     /gene="rpmC"
                     /locus_tag="PK109_01190"
     CDS             complement(3680..3874)
                     /gene="rpmC"
                     /locus_tag="PK109_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRH24191.1"
                     /translation="MAKKISLKEKNAGELVKLLGERREELRKLRFEAAGARPKDSNAP
                     AKVKRDIARIMTELHAKKTA"
     gene            complement(3879..4298)
                     /gene="rplP"
                     /locus_tag="PK109_01195"
     CDS             complement(3879..4298)
                     /gene="rplP"
                     /locus_tag="PK109_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRH24192.1"
                     /translation="MLFPKKVKHRKWQTGRKSPAKLARPDTRGTSVAFGSHGLKATTA
                     SRVTSNQIEAARKVLTRVTGKGGKLWIRIFPDRPVTAKPAEVGMGSGKGDPKHFVFEV
                     RPGRILFELDGVEDAVARDAMRRAGSKLPVKSTVITR"
     gene            complement(4305..5012)
                     /gene="rpsC"
                     /locus_tag="PK109_01200"
     CDS             complement(4305..5012)
                     /gene="rpsC"
                     /locus_tag="PK109_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRH24193.1"
                     /translation="MTHTVHPYAHRLGIIRDWKSRWFAGDKKSYRENIVVDEALRSYL
                     SKKLRASYVADIEIERGAAEIRVVLHTARPGLVIGRSGENAGKLRKELSDVVRKVAPN
                     HNRSVKLDIMEIRQPETNAAVVGYMIAEGLEKRLPFRRVLKMTVEKVMAVREVIGVRI
                     VISGRLGGADMSRTEQIKKGRIPLQTFRANIDYAQVEAHLPYGQIGIKVWVYLGDTFA
                     PAERPAAPAPARTNSPR"
     gene            complement(5018..5341)
                     /gene="rplV"
                     /locus_tag="PK109_01205"
     CDS             complement(5018..5341)
                     /gene="rplV"
                     /locus_tag="PK109_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011183025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRH24194.1"
                     /translation="MKAELKSHNAPPRKVRLVTDLVKGKKVPAALAALSFLPKRAAEP
                     IRKLIASAAANAKNQGENPDQLIVKTIRVDSAGMLVKFMPRAHGRAAPIRRRKSRVQI
                     ELASK"
     gene            complement(5345..5683)
                     /gene="rpsS"
                     /locus_tag="PK109_01210"
     CDS             complement(5345..5683)
                     /gene="rpsS"
                     /locus_tag="PK109_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRH24195.1"
                     /translation="MSRSLKKGPYVDVKLLKKVQGKKPADTGVIKTWARNSQIAPEFV
                     GFTFGVHNGNKHIEVLVTEDMVGHKLSEFSPTKKFVRHGGKMQKELEQKKKDSEIAAA
                     KASKAEPAKK"
     gene            5918..6757
                     /locus_tag="PK109_01215"
     CDS             5918..6757
                     /locus_tag="PK109_01215"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HRH24196.1"
                     /translation="MPPETAPAPVDAPVPPHPQPIPLLIALALLLIAGGVLWYILTRP
                     SPQIPLGQTATSTPEGGVTGAKHITEETDLYEIDAAYPAATPLKASAGAAADAAAVSA
                     MKTFAEQEIARFKDNGEFDSMTAEDKAMLGYGDGRKQALGLEYKTYTSPATVSYVYLI
                     YVDTFGAHPNAYYRTFTFDAATGEELHTDDLFTPTADYTSVLSSETRTRLKASLGADA
                     AIDMLEAGTTPDADNFQNFYLDGNALVIVFPPYQVGPYAIGTQEVRIPRASLGNALAA
                     RYR"
     gene            7012..7944
                     /locus_tag="PK109_01220"
     CDS             7012..7944
                     /locus_tag="PK109_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24197.1"
                     /translation="MGEGWKDIFKFGRSHQPESVPSVVEKNFAPLTAQLDTELAQSEL
                     KDDALLYEAVGGTVGMVAAESTALAHQGDSAYTPQSISALRAAFVEAGTKAAGAFKDI
                     FTYDETNGNTVVEGSATETAAKVKEAKGQPKFINAQMLPRVVNSIDPTATPESIASAI
                     EKADFFAVRTMVAKMLGMNEQRAKETQRDAAGTQQFLKTVLKPDDYFLSLVDTIKEPG
                     AAPSVQDVTTLELGFRLANVTWASGQPLIALYEGENSERGNSEKQDNFRAFDDMVAKM
                     YNEGGLDRATFEVMKDLEQLKEFVKQVREAETKA"
     gene            complement(7992..>8503)
                     /locus_tag="PK109_01225"
     CDS             complement(7992..>8503)
                     /locus_tag="PK109_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRH24198.1"
                     /translation="VPESAERKHALWNEGVNYASDAVVTRKTDKGDLEILLILRKDGS
                     WALPGGFRDTVDGEQEAAEVTMLREALEETKLDFSSFPVTKVYEGIVDDKRNEGTPGE
                     PGSRWIETAAFHIDATGHSELVPVGGDDAQSAKWQIVTPELLDNLYASHGKIVRDTLT
                     QIGEALDDF"
BASE COUNT         1842 a         2278 c         2258 g         2025 t
ORIGIN      
        1 atagccaccg cgatcaaaaa cgatagcggt aaccccagcc ttcgtggcac gctctgcgat
       61 agccttcccc actgctcctg cctgcttccc gagagatcct gggaactcgc ggccatgtgc
      121 agaaacgagc gtctttcctg ctgcgtcatc aataacctgc gcgctgatga acttgttgga
      181 gcggaatacg gcaaggcgag gacgctcggc agtgccggta atgcgtgcgc gaatacgtga
      241 gtgacgacgt gcgcgaagtg ctgatttaga aggagttgcc ataaaagatt agacggcttt
      301 cttgccctgc ttgcgcagta ttacctcatc atcgtagcga ataccctttc caaggtacgg
      361 ctccggcggc tttacggcgc gaatctctgc cgcaaactga ccgacacgct gcttatcagc
      421 gccttcgata acgatgttgt tcttagtaac ggtagccgta aggccttcag gaataggaag
      481 ggtgacctga tgagagaatc caacgctcaa gacaaggtcc ttacccttga gttctacctt
      541 gtacccaacg cccgtgagga tgagggtctt cttaaacggc gtctctactc cctgcatcat
      601 cgcctttacg tgtgcggcaa acgtaccggt caacgcgtgt gcgagacgtg aggtgttctt
      661 aggagtgatc tggacacctt caggtccaac gacgatagaa atagaagagt gcaccgcctt
      721 ggtgagcgtt tccttagatc cctttacggt gagcataccg tcagaggagg ttacctgaac
      781 ctttcctggg atagcgatgg gcttttttgc gaggcgggac atacaagtat taccagattt
      841 caaacaaggt ctctccaccg gcgcgtacct tgcgggcctc agcgtcagaa agcactccct
      901 tcggcgtgga catgatgcga gcaccaagac cgttcttcac cgagtgagca agctttgcgg
      961 gagcgtagag gcggcggcct ggcttactga tgcgctttac gccgttaatc ttgtgttccc
     1021 cacgctcggt gtacgcaaga gcgacctcaa gcatcttgtg gtccttctta gaaacagata
     1081 cgtcagcgac gaagccgagc tgcttgagct tcttagcaat cgcctcaagg tggtttgagt
     1141 gcggcaatga aatagaagca gtgccgatag cgcctgcatt cttaagacgg atgatgaaat
     1201 ctcctacgcg gtcagatacc ataaaaagtt accaagaagc tttcttaacg cccgggatct
     1261 taccgtcgcg ggcaagctcg cggaactgga tgcgggagag accaaacgcg cgcaggtagc
     1321 cacggccacg gccagagata gagcaacggt tcttaacccg gatcggtgag gagttcttag
     1381 ggagcttctg gaggccctca tagtcccctg cggccttaag ctcagcgcgc ttagcagcgt
     1441 acttcgcgac gagctttact cgtttgcgat tgcgggctag ttgtgaggtt ttggccatga
     1501 ggggttatag gggcacccca gctggctcgg gaaccgtcag gaagaatcct ggggttatcc
     1561 tcgctcgtgg gcattaaaaa atgccgctga gggcttaagg ggatactagc agtctctcta
     1621 gagcgtgtca aacaaagggg cgggtgccgt tacagctacc cgccccctgc ctttgtattc
     1681 gagctccttc actcaacagt gccgaacagt ggcgttggaa atacgagagc gcctcggaag
     1741 gcaccccgcg ttccattgtc gttcgggacg ccgcgaggcg ttatcccgtt tgcgtccgtg
     1801 ccggtaaggc gtgtccctac ggacacgccc tcgtcagttc caaagagcct tcgtgtgtac
     1861 acgtactggc gttcgccccc atcgttttca taggaacgtt gtacgccgtt acgttgtctc
     1921 cattcgtcgt gtcgagattc tcgaaacgat cttcttccga tcaccacgag cacattctgt
     1981 gggacttcag agtctcccgc ataaaacggg acggttgaag cgtagtcagt cacaggcgct
     2041 tcctcctgcg catagctacc cgttggtatc gcaacgagca caaagatcag agtcgttatt
     2101 tttttcatct gtcatcctcc gagcaatgac aggttgacga tacgcccnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnata ccgatggtga ggtttcccat ctgatcgact
     2281 gccgtagggg caatgccgcg gaagtcgcgc gtacgaggaa gcgcgatgtg gatgagcttg
     2341 tcgaggaact cgtacatgcg tgcgccacga agcgtcacct ggtagccgat gatgtcgcct
     2401 tcgcgcatct tgaactgagc gatagaagtc ttcgctgggc gcgctgatgg cttctgaccc
     2461 gtgatgcggg caagcttctc atcaacgagc ttgttgcgtg ccttgtcttt ggccttaccg
     2521 gtgccagacg acacgaccac cttaaggata cgtggtgcct ggaacttgtt cgtgtacccc
     2581 tgactctcgg cgagagcttc gtaggcgttc tgctgttttt gttttgaaag catcataatg
     2641 ttggaggcta cttgatgacg gtcttactct tctttgccat gcgaaccgtc ttgccgttct
     2701 gcttctcacg gccaatgcgc gttggcttct tggtctcagg atcaaggatc atgacgttag
     2761 aagcgttcac ggatgctggg cgctcgacga tagcaccgga ctgcttgcga cccgtgcggc
     2821 ggaggtgacg cttcaccatc gcaataccgt cgacgacgac gcgacccgtt gatgggatag
     2881 ccaccaagac aacgccggtc tttcccttgt cttttccaga catgagcatg acggtatcgc
     2941 ccttcttgat tttcatctta gcaaccgcgt gacgattcac cttttggaat cgcttcttct
     3001 tcgaggcgta agacttctgc ttggtatcgg tgtactttct catatgattt agacgatttc
     3061 tggggcgagt gacgcaatgt tctgccagcc acgctccgtg agctcacgag ccaccggacc
     3121 gaagacgcgg ttccctttcg gacccatctc cttaccctgc ttctcgatga taacgacagc
     3181 gttatcgtca aagcgaatat aggaaccgtt ggcgcggcgg aatgccttgg tctgacgaac
     3241 caccactgct ttgcagacat ccttcttctt ggtggccttg cggggttctg cggtctgaat
     3301 ggagatgaca acgacgtcac cgaggccagc gtagcggcgc tttgagcctc cgagcacctt
     3361 gaacacgcgg cccaccttgg cgccagtgtt gtcagctacg gtaacgatag agcggtcttg
     3421 aatcataatt agttggctat cttgaagcgc ttgagcttgc tgatagggcg gcacgcaacg
     3481 atggtgacgc gatcccctac ctgaaccgtg ttacccggat catgcgcaag atatttcttg
     3541 gtgcgggtct ggtacttctt gtacttcgga tgcttcacgt agcgagacac cgcgaccgta
     3601 gccgtgtcct tcatcgccgt ctttacgacg acgcccttga agctctgagg ttttgagata
     3661 gttggttcca taaaataagt tatgcggtct tcttcgcgtg caattcggtc atgatgcgag
     3721 caatgtcgcg cttcaccttc gccggagcat tgctgtcctt cgggcgtgca ccggcagctt
     3781 caaagcgaag cttgcggagt tcttcgcggc gctcgccgag aagctttacg agctcccctg
     3841 cattcttttc cttgagtgag attttctttg ccataaattt agcgggtgat gacggtggac
     3901 ttcaccggaa gctttgagcc agcgcggcgc atcgcgtcgc gagcaactgc gtcctcaaca
     3961 ccatcgagct caaagaggat gcgtcctgga cgtacctcga acacgaagtg ctttggatct
     4021 cctttacccg aacccatgcc cacttcagct ggcttagcgg tgaccgggcg gtctgggaag
     4081 atgcggatcc agagtttacc gcctttaccc gtaacgcgag tgagtacttt gcgggcggct
     4141 tcaatctggt ttgaggtcac gcgagacgca gtggttgcct taaggccatg ggacccgaac
     4201 gcaacggaag taccgcgcgt atcaggacgt gcaagcttcg ctggggactt tcgtccggtc
     4261 tgccacttgc ggtgcttaac ttttttaggg aatagcatag gcgtttatcg aggagagttc
     4321 gtacgagcag gagcaggtgc cgctggacgc tccgctggtg cgaaggtatc gcccaagtac
     4381 acccagacct tgatgccgat ctgaccgtac gggaggtgtg cttctacctg agcgtagtca
     4441 atgtttgcac ggaaggtctg aagcggaatg cgtcccttct tgatctgctc cgtgcggctc
     4501 atatcagcgc caccaaggcg accagagata acgatgcgca caccaatgac ttcgcgcacc
     4561 gccatcacct tctctaccgt catcttgagg acgcggcgga atgggaggcg cttctcaagg
     4621 ccctcagcaa tcatgtagcc gactaccgca gcgttcgttt ctggctggcg gatttccatg
     4681 atgtcgagct tcactgagcg gttgtggttc ggagccacct tgcggacaac gtctgagagt
     4741 tccttgcgga gcttaccggc gttctcgccg gagcgaccaa tcacgagtcc tgggcgtgcg
     4801 gtgtggagca cgacgcggat ttcagcagcg ccacgctcga tttcaatatc agcaacatag
     4861 gaagcgcgga gcttcttaga aaggtaggag cgaagcgctt cgtcaacgac gatgttctcg
     4921 cggtagctct tcttgtcgcc agcaaaccag cggctcttcc aatcacggat gatgccgagg
     4981 cggtgtgcgt aaggatgtac ggtatgagtc ataaaaatta tttggaagcg agctcaatct
     5041 ggacacggct cttgcggcgg cgaatcggcg ctgcacgacc atgagcacgc ggcatgaact
     5101 taacgagcat accggctgag tcaacgcgaa tggtcttcac gatgagctga tctgggttct
     5161 caccctggtt cttagcgtta gcagcagcag acgcaatcaa cttgcggata ggctctgcag
     5221 cgcgctttgg gaggaatgag agtgctgcga gcgccgctgg caccttcttt cccttcacga
     5281 ggtccgttac gaggcggact ttgcgcggag gagcattatg agacttaagt tcagctttca
     5341 taggttattt cttggcaggc tcagctttgg aagcctttgc tgcagcgatt tcagagtcct
     5401 tcttcttctg ctcgagctcc ttctgcatct ttcctccgtg acgaacgaac ttcttcgttg
     5461 gagagaactc agaaagcttg tggcccacca tgtcctcggt gacaagcact tcaatgtgct
     5521 tgttaccgtt atggacaccg aaggtgaatc ctacgaattc aggagcgatt tgagagttgc
     5581 gcgcccacgt cttaatgacc ccggtatcag ccggtttctt tccctggact ttcttgagga
     5641 gttttacgtc cacgtagggt ccttttttga gtgatcgtga cattggtgtg ggttgggttt
     5701 tgtggggcgg tcctaacaaa ctagcccgtg caccggaggc tatacctcca tgtgcccggg
     5761 tagcgaacgt tcgaaccagc ccccttttgc tttttcagca aaaacccgcg caaggcggat
     5821 aggactaatc tacaggtcgg ggggaagggt gtcaaatatt cctgaaaata cggggattca
     5881 tgaaagcctc atggagccta tgggatatca tagggatatg cctcccgaaa ccgcacccgc
     5941 tcccgtagac gctcctgttc ctcctcatcc gcagcctatt cctttactca ttgcccttgc
     6001 cctcctcctt atcgcaggcg gggtcctctg gtacatcctt acccgccctt ccccacaaat
     6061 ccccttgggg cagaccgcaa cctccacccc agaaggcggc gttacgggcg ccaagcacat
     6121 tacggaggag actgatctct atgaaataga cgcagcgtac cccgccgcta ccccactcaa
     6181 agcatcagca ggagccgcag cagatgccgc tgccgtgagt gcgatgaaaa cctttgccga
     6241 gcaagagatt gcccgcttta aggataacgg tgagtttgat tcgatgactg cagaagataa
     6301 ggcgatgctc ggatatgggg atgggcggaa gcaggcgctt ggtcttgagt acaagaccta
     6361 cacctccccc gccacggtct cctacgtcta ccttatctac gtcgacacct ttggtgcgca
     6421 tccaaacgcg tactaccgca cctttacctt cgacgctgct accggcgaag agcttcacac
     6481 tgacgacctc ttcaccccaa ctgctgacta cacaagcgtc ctgtcttccg aaacgagaac
     6541 ccgcctcaag gcatcgttgg gggccgatgc tgctatcgac atgcttgagg cagggactac
     6601 ccctgacgcg gataacttcc agaacttcta ccttgatgga aatgcgctcg tcatagtctt
     6661 ccctccgtac caggtggggc cctacgccat aggcacccag gaagtacgta ttcctagagc
     6721 gtctctaggt aatgcactag cggcccgcta ccgctagcag cgaccctata tacttaccct
     6781 cacacctggt tggggagaaa ggagtttccc atgaaaaaac cagtgttgcg tattaaccga
     6841 atgccggcac tttggggaag cattatccct gcgattgctt tcattttgct tggattggcg
     6901 attctttctg tattccatcc gagcttaggt taacgccggc ggccacccct ttagggtggc
     6961 cgcttgctta tatgggatat actttctcag taacaaataa cttatagcgc tatgggagaa
     7021 ggatggaaag atatatttaa atttggccga tcacaccagc cggaatcagt tccatcagtt
     7081 gtagagaaaa actttgcacc gcttactgct cagcttgata ccgagcttgc gcaaagcgaa
     7141 cttaaagatg atgcacttct ctatgaagct gtgggcggaa cggtaggtat ggtagcggca
     7201 gagtctaccg cacttgccca ccagggagat tctgcgtata cccctcaatc tatttccgcg
     7261 cttcgtgccg cattcgttga agcaggaacg aaggctgccg gtgcattcaa agatattttc
     7321 acgtatgacg aaactaatgg gaataccgta gtggaaggca gcgcaaccga aacggcagca
     7381 aaagtgaaag aagcgaaagg acagcctaaa ttcataaacg cacagatgct gcctcgtgtg
     7441 gtgaactcca tagaccctac cgccaccccg gagtctatag catcagctat tgaaaaagca
     7501 gacttctttg ccgtacgcac tatggtcgca aaaatgcttg gcatgaatga acagcgtgcc
     7561 aaagaaaccc agagagacgc tgcgggtacc cagcaattcc tgaaaacagt cttgaagccg
     7621 gatgattact tcttaagcct cgtggatact attaaggaac cgggagcggc tccgagcgtc
     7681 caagacgtaa caactcttga gcttggattc cgtcttgcaa atgtaacctg ggcctctggg
     7741 caaccgttga ttgcgctcta cgaaggcgag aattcagaac gtggaaattc agagaagcag
     7801 gataatttcc gtgcgttcga tgacatggtc gcaaaaatgt acaacgaagg aggtcttgat
     7861 cgtgcgacgt tcgaagtaat gaaagatctc gagcaattga aagagtttgt gaagcaagtg
     7921 agggaagctg aaacaaaagc atagaaaaag ccccgcatac gcggggcttt ttctatctac
     7981 gagatgattt cttagaaatc atctagtgct tcccctatct gcgtgagagt atctcgaact
     8041 attttcccgt gactggcata aaggttatcg agaagttctg gagtaactat ctgccactta
     8101 gcgctttgtg cgtcgtcacc tcctaccggc acaagttcag agtgacccgt tgcatctatg
     8161 tgaaaggcag cggtctctat ccatcggctc cctggctccc ctggcgttcc ttcgttacgc
     8221 ttatcgtcaa cgataccttc gtataccttc gtcacaggga aagaagagaa atcaagtttt
     8281 gtttcttcaa gcgcttcacg aagcatggta acttctgcag cctcttgttc tccatcaacg
     8341 gtatcgcgga atcctccggg aagcgcccac gagccgtcct ttctcaatat gaggaggatc
     8401 tccagatctc ccttatctgt ctttctagtt actacggcgt ccgacgcgta gttcacccct
     8461 tcattccata atgcatgctt cctctcggct gattctggga cag
//
LOCUS       DAORIN010000016         8429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1481_length_8429_cov_4.303200, whole genome shotgun sequence.
ACCESSION   DAORIN010000016 DAORIN010000000
VERSION     DAORIN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1481_length_8429_cov_4.303200"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3986)
                     /locus_tag="PK109_01230"
     CDS             complement(<1..3986)
                     /locus_tag="PK109_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HRH24199.1"
                     /translation="MNPRAFALVKTLKAFQAVMLAVTLVTLSFPVTPLTARAQEDTTP
                     TEEVVVEEATTPEEQAPEESTQEGENTLNLDVVLDTQNNNVDICHKPGTPAAQVLNVN
                     SNAVSGHTGHGDYVIDAQHPASGCVATEEPKDPPYTYDDETEDDMCVVVSDTYTIEGG
                     QPAVLVSNPHSAWTAVLASISKWIWGEDPTTLTAGDEVETFTRTFSVDEVPSGATLRV
                     AADNGYSVTVNGHAVGADPEEHNYQAGHEDNYSIPAADLLEGENAITFVVTNKDLSGE
                     TPANNPGGLRYVLTVNGADCGEPKTPENSCVAPSTLETTGTQTFGGSEETLQHILDNA
                     TPSYSVDADDDQTNTQQWNGTGNNVDFTFKYVKGIAANKHVFGYFLNGGAFQPIFKDG
                     NVTGYAATPTAVVGSTYNLSLTNVSDVVFAIYDGTSYYYTKNTSNGDGKRHAVVYNSD
                     NNEYVVAFEDLPNQGDKDFNDLVVEIGVTGCETPEDSITVVATKIVCDDESDLPNVMG
                     SNHQTTADTAADFLETHGDSCHLEEGWQFQWCDQNDGDAGRAFTGEAAGYTTSGLTAE
                     DGTVSMTIPLEGITEVHLREVLKPGYIPFSFDQTGGDNSNVSAEFACSNDGLNYDNWD
                     FIRSPEEGKTYYCVAWNVEKEKAPTCDPKINLVANGGFENPDVTNGAGWDVFEVGTSP
                     ALVWLADFVGAASGGKVEIHGGVNGWLASEGSQYTELDSDLNGPSNPGGQSAVEIAQG
                     LTTVNGQKYHVSYDFSARPGTAKEQNGVTVSVNGVPMQTTNAANATAGNQTEWDSYGF
                     DFTAGGTLTTLALRDAGNANDSLGSFVDNVTVTCVPDEQGEDTAETLVVTPFASQGWS
                     LFNYDIADGSDDANPTTSGTDGDFVTGPATPPLQTGSFNQKVVNGDDATRLMSSDYNG
                     TPLEDITKLEYSTYVTANAGAQATYIQIRIDRDADGTWDDALFFEPVYQSGTYGMLAY
                     SPDAVPDQCDGNPNCVAIGAWQDWDADAGGWWSNNDSAGGPPLTTLADYAAEYPGAVL
                     ATDVPAIRIQTGFGGAAWQNFDGNFDKFVIAVKTGSNTHTTTYDFEPAEDDGCFDDER
                     DVRVDCGGDDEGRLVIIKEVDGEDASFDFDIVAGEFFADADVTTTGRSGSTELYLSEG
                     TYDVTESVFEGYSLDSVTCEYEGESEGVNIVNGERIYIEEGETVTCTFHNTQDEQFDG
                     PTSRSGSGRGRSGGSSGDGEVLGASTCGPLLTDYLKMGWANSPDEVTKLQQFLNDQLG
                     VTLPLSGFFGPMTFDAVKAFQLAHAKDILIPWVGLPGSGITGENTPTGFVYQTTRWQI
                     NNIWCPGS"
     gene            4362..5645
                     /locus_tag="PK109_01235"
     CDS             4362..5645
                     /locus_tag="PK109_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRH24200.1"
                     /translation="MKRVMSPSMSGAHSSPVMPAVSAASPLSLEQELDRGADISIVNL
                     LDCLVTKAHTLNSSDIHLDPTRTGVRVRFRLDGVLKDFQELPKDVHQEVISRIKVLAS
                     LRTDEHQAAQDGRFRHTFADGVCVDIRVSIAPTYHGENAVLRLLSGQRTEHTLSMLGF
                     SEKDKEKVERAISKSNGMILATGPTGSGKTTTLYTLIKMLNRPEVAIVTIEDPIEYSV
                     DGIEQIQVNARTSLTFANGLRSVLRQDPDIIMVGEIRDAETAGIAVNTALTGHLLLST
                     LHTNDAITTLPRLSDMGIDPYLVASTMNIAIAQRLVRKTCEACKAECAMTDVMKASLV
                     DLPVQKPLQAGEKIYKGKGCDACAGSGYKGRVCINEVLVATPAIREAILRKASADEMR
                     AIAIREGMTTMLDDGFEKVRSGTTTIEEILRVIHD"
     gene            5638..6861
                     /locus_tag="PK109_01240"
     CDS             5638..6861
                     /locus_tag="PK109_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRH24201.1"
                     /translation="MINRELIVAHTLGAGKQVIALSYATVTTLHKHSEPVRRSFKKLS
                     DKYIEMRNAYELKSAPRLSVKDQMFFAKRLSFLIKAGVPILESLHMISSQTASRQSAR
                     VIGSVYQDISNGSSLAKSLGKFPHIFGDFGINIIKIGESSGTLSTNLEYLADELKKRH
                     SLQGKVVSAFIYPGIITLATLGITAFLMLYLFPKIMPIFSSLHMELPLTTKIVMAISL
                     FLQAWGLLLIGVIIAACIGISLLKKKNKQFHFFFDQVVLSMPLFGPLMRYYNLANGTR
                     TLGILLQGGVTLSEALPLTADTTANLVYKAEYRKMAEYIHRGERMSSFLVTSENLFPP
                     IMAQLIAVGERSGNLSNTLVYLSELYEAEVEDFTKNLSSLIEPALMVIMGILVGFVAI
                     SIITPIYGITQNLHT"
     gene            6833..7321
                     /locus_tag="PK109_01245"
     CDS             6833..7321
                     /locus_tag="PK109_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRH24202.1"
                     /translation="MASRKISIPKGFTLLEILVVMGLVLMVVAFGLIVSFDDYRGFTF
                     RNERDIVVSVIQKARGQAINNMCFGAGCTDGKAHGVYFGTPGEYVVFQGTSYATRDVA
                     LDEVIAARDNASVVSGMTSVVFNRLRADTTTVGGTTLTITQPGHTSTITVEPEGRVWW
                     TN"
     gene            7323..>8429
                     /locus_tag="PK109_01250"
     CDS             7323..>8429
                     /locus_tag="PK109_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRH24203.1"
                     /translation="MMTPRGFSTLEMLIAMTVLLLAFTATTLLLPGIQDSSIDTEIAV
                     EALNIAERTLENQQALARKDFKLVTGSSSQETIGGTVYQKTVTAASTDYFTKQITALV
                     TWGGMYGRAQSITLKSVVSNFEESIGGDTCDSILTGNWLLPPQITNRTLGAQIMGDGS
                     GLYPITDLDAYRKRLYVTVNNTGTLPSSGPRDAGTAANTSGVGTVSWGNPTNARTSDN
                     SYATASLSGTQATQYIKATNFGFTIPQDATIVGIKVDIERKASSNTNTVRDNVVRLVR
                     ADGSLGGTNQAHTTSWPTSDAADSYGSATDLWNESGWSPAAINDADFGAVISAIANGG
                     SGTRTASVDSVRITVTYTRQLYVLNATTPTNPTFD"
BASE COUNT         1884 a         2366 c         2177 g         2002 t
ORIGIN      
        1 tcagaacctg ggcaccagat gttgttgatc tgccagcggg tcgtctgata gacgaagccg
       61 gttggcgtgt tctcaccggt aataccagag cctggaaggc caacccacgg aatcagaatg
      121 tcctttgcgt gtgcaagctg gaatgctttc accgcatcga aggtcattgg gccgaagaag
      181 ccagagagag gaagcgtgac gccaagctga tcgttaagga actgctggag cttcgttacc
      241 tcatctgggc tatttgccca acccatcttc aagtagtccg tgagaagcgg gccgcaggtg
      301 cttgcaccca agacttcgcc gtcgccagac gagccgccgg agcgaccgcg tccggaacca
      361 ctgcgagagg tagggccgtc gaactgctca tcctgcgtgt tgtggaaggt acacgttacc
      421 gtctctcctt cctcaatgta gatgcgctca ccgttcacga tgttgacgcc ttcagactca
      481 ccttcgtatt cgcaggtaac gctgtcgagc gaatagcctt cgaatacgct ttcagtaacg
      541 tcgtaggttc cttcagagag gtagagctcg gttgaaccgc ttcttccagt agtagtgaca
      601 tcggcgtcag cgaagaattc gccggcaacg atatcaaagt cgaaggatgc gtcttcgcca
      661 tcaacctcct tgatgatcac cagtctgccc tcgtcatcgc cgccgcaatc gacgcgcaca
      721 tcgcgctcgt cgtcgaagca gccatcgtcc tccgcaggtt cgaagtcata cgtagtcgtg
      781 tgagtgttcg agccagtttt tacagcgatg acgaacttgt cgaagtttcc gtcgaagttc
      841 tgccacgccg ctcctccgaa tccagtctgg atgcggattg caggaacgtc agttgcaagt
      901 actgcgcctg gatactcggc agcataatca gcgagcgtcg tgagcggagg gccgccggcg
      961 ctatcgttgt tagaccacca tcctcctgcg tcagcatccc agtcctgcca tgcgcctata
     1021 gcgacacagt tcgggttgcc atcgcattga tccggaacag catctggact gtaggcaagc
     1081 atgccgtacg tgccactctg gtataccggc tcaaagaaga gtgcgtcatc ccaagttcca
     1141 tctgcatcgc gatcgatgcg gatctgaatg taggtagcct gtgcaccagc atttgctgtt
     1201 acataggtgc tgtactcgag cttcgtgatg tcttcaagcg gtgtgccgtt gtagtcagat
     1261 gacataaggc gcgtagcatc atccccatta acaaccttct gattgaagct gcccgtctgt
     1321 agcggcggcg ttgctgggcc ggtgacgaag tcgccatcag ttcccgacgt cgtcggatta
     1381 gcatcgtcac taccgtctgc aatgtcgtag ttgaagaggc tccaaccctg cgatgcgaac
     1441 ggagtgacaa cgagcgtctc cgccgtatct tcaccctgtt catcaggaac gcaggtaacc
     1501 gtcacattgt cgacgaagga accgagcgaa tcgttcgcat ttcctgcatc gcgaagtgcg
     1561 agcgtggtaa gcgttccgcc agccgtgaag tcgaatccat aggagtccca ctcggtctgg
     1621 tttcctgccg ttgcgtttgc agcattcgtg gtctgcatcg gtacgccgtt tacgcttacc
     1681 gtcacgccgt tctgctcctt agcagttcct gggcgagcag agaagtcata cgagacgtgg
     1741 tacttctgac cgttaaccgt cgtaaggcct tgagcaatct caaccgcaga ctgtccgcct
     1801 gggtttgatg gaccgttcaa gtcagagtcg agttcagtgt actgcgagcc ttcgcttgca
     1861 agccaaccgt tcacgcctcc atgaatttca acctttccac cagatgctgc gccgacaaag
     1921 tctgcgagcc atacgagtgc tggcgaagtg ccaacctcga atacatccca gcctgcgcca
     1981 ttagttacat cagggttttc gaaaccacca tttgcaacaa gattaatctt aggatcgcag
     2041 gtcggcgctt tctccttctc tacgttccaa gcgacgcagt agtaggtctt accttcttca
     2101 ggactgcgga tgaagtccca gttatcgtag ttcaatccat cgttcgaaca tgcgaattcg
     2161 gccgaaacat tgctgttatc gccacccgtt tgatcgaagc taaatggaat gtagccaggc
     2221 ttcaagactt cgcgaagatg tacttccgtt atgccttcaa gcggaatggt catagagacc
     2281 gttccgtctt cagcagtgag gccagacgtg gtgtaccctg ctgcttcgcc agtaaaggcg
     2341 cggccagcgt cgccatcgtt ttgatcacac cactggaact gccaaccttc ttcgagatgg
     2401 caggaatcac catgagtctc caagaagtct gctgcagtgt cggcagtagt ttgatggtta
     2461 gaacccataa cgttcggaag gtctgactcg tcatcacaga caatcttcgt tgcgactaca
     2521 gtgatgctgt cttccggagt ttcgcagcca gtaacaccga tttcaacaac gaggtcgttg
     2581 aaatccttgt caccctggtt tggtagatct tcgaatgcaa cgacgtattc gttgttatct
     2641 gagttgtaaa cgactgcatg tcgcttaccg tctccgttcg aagtgttctt ggtgtagtag
     2701 tagctggtgc catcataaat ggcaaagact acgtcagata cattcgtgag agagaggttg
     2761 tacgtactgc caactactgc agttggggtt gctgcatacc cagtaacatt tccatcctta
     2821 aatataggtt ggaaagctcc tccgtttagg aagtacccga acacgtgctt gtttgcagcg
     2881 attcccttca catacttgaa agtgaagtcg acgttgttgc ctgtaccatt ccactgttgc
     2941 gtgttggtct ggtcatcatc agcatccact gagtaagacg gcgtcgcgtt atcgaggatg
     3001 tgctgaagtg tttcttcgga acctccaaac gtctgcgtgc ccgtggtttc aagagtcgac
     3061 ggcgcgacgc atgagttctc aggagtctta ggctcgccgc agtctgcacc gttaacggtg
     3121 aggacatagc gaagaccgcc cggattgttt gccggagtct cgccggacag gtccttgttc
     3181 gttactacga aggtaatggc gttctcacct tcaaggagat cagcagctgg aattgagtag
     3241 ttatcctcat gaccagcctg gtagttatgc tcttctgggt cagcaccgac cgcatgaccg
     3301 ttaacggtaa cgctgtagcc gttgtctgcc gcgacgcgaa gcgtagcgcc agaaggaact
     3361 tcatctactg agaaggtacg ggtgaaggtt tcaacctcgt cccctgcagt aagcgtcgtc
     3421 gggtcttcgc cccagatcca cttagagatg gatgcaagaa ctgcggtcca tgctgaatgc
     3481 gggttcgaga cgagaacggc tggctggccg ccttcaatgg tgtacgtgtc gcttacgaca
     3541 acgcacatat catcttccgt ctcgtcgtcg taggtgtacg gaggatcctt tggttcttcc
     3601 gtagctacgc agcctgatgc tgggtgctgt gcgtcaataa catagtcgcc atgaccggtg
     3661 tggccagaaa cggcattgct attaacgttc agtacctgtg ccgctggagt gccgggctta
     3721 tggcagatgt ctacattatt gttttgcgta tcgagcacaa catcaagatt gagagtgttt
     3781 tctccttcct gagtcgattc ttctggagcc tgctcttcag gagtggtcgc ctcttcaacg
     3841 actacttctt cagtcggcgt ggtgtcttcc tgggcacgtg ccgtaagcgg cgtgaccggg
     3901 aatgacaagg taaccagggt cacagcaagc atgacggcct ggaacgcctt gagcgtctta
     3961 acgagcgcga atgcgcgtgg attcatatgc atggataatc cgtttattaa tttgtatgta
     4021 gtgataatgg aaacgcgcac gcaccaacga actttccgaa taagggaaag tgcgacggcg
     4081 agactctttg cccgacgtac tgctacgtct gcatgtagta gtactcctac ccccgtgagg
     4141 tgtcaatgaa gagagaaacg aaaaaatggc ctatttaaag gacaatttga gcgttgtgtc
     4201 ctttatgaag acagtgtcca ataaaaagta ttgtatttct ttgctttcgt gttttgagtg
     4261 tcctatggga taggaagtga gctacgtcga gtatacggaa gacgggtgtg gataactctt
     4321 cctctggact tcttctcttt tcattgctct agacaaagtt catgaagaga gtaatgtcac
     4381 cctcaatgag cggcgcacat tcctcaccgg ttatgccggc cgtttcggcc gcgtctccgc
     4441 tttcccttga acaggagctt gatcgtggcg ctgatatctc catagttaac ctgctcgact
     4501 gcctcgttac gaaggctcat acgcttaatt cctccgatat tcaccttgat cctacccgta
     4561 ccggggttcg ggtgcgcttc cgtctcgatg gcgtactgaa agatttccag gaactcccta
     4621 aagacgtaca tcaggaagtc atttcccgca taaaagtgct cgcaagcctc agaaccgatg
     4681 agcatcaggc ggcgcaggac ggacgattcc gtcatacgtt tgctgacggc gtctgcgtcg
     4741 acattcgcgt ctctattgcg cctacctatc acggagaaaa tgccgtcctc cgtcttcttt
     4801 ccggccagag aactgagcac accctctcta tgctcggctt cagcgagaag gataaagaaa
     4861 aggtagagcg cgctataagc aaatcaaacg ggatgatctt agcgacgggc cctaccggct
     4921 ccggtaagac gacgaccctc tataccctta ttaagatgct gaatcgtcct gaggtcgcta
     4981 tcgtcactat cgaagaccct atcgagtact cagtggacgg tatcgagcag attcaggtga
     5041 atgcccgcac ctcactcacc ttcgccaacg gccttcgctc cgtcttacgt caggaccccg
     5101 acatcatcat ggtgggagaa attcgtgacg ctgaaactgc tggtattgcg gtgaacactg
     5161 cgcttaccgg tcaccttctc ctttcaacgc tccacaccaa cgacgccatc accacccttc
     5221 cccgcctttc tgatatggga atcgatccct atctcgtcgc ctcgacgatg aacattgcta
     5281 ttgcgcagcg cctcgtgcgt aagacgtgtg aggcgtgcaa ggctgagtgc gcgatgacgg
     5341 atgtcatgaa ggcaagcttg gtagatcttc cggttcagaa gccgcttcag gcaggagaga
     5401 agatatataa aggtaaggga tgtgacgcgt gtgcggggag cggctacaaa ggacgtgtgt
     5461 gtattaacga ggtactggtt gctaccccgg caattcgcga agccatactg cgcaaagcgt
     5521 cagccgacga gatgcgcgct atcgccatac gcgaaggaat gaccacgatg cttgacgatg
     5581 ggtttgagaa agtgcgcagt ggcactacta ctatcgagga aatcctccgc gtcatccatg
     5641 attaatcgag aacttatcgt cgcccacacc ctaggagccg gaaagcaggt catcgcgctt
     5701 tcctacgcta ccgttactac gctccacaaa catagcgaac cagtgcgtcg ctcgttcaag
     5761 aagctttcag ataaatacat cgaaatgcgc aatgcatacg agcttaagag cgcaccgcgt
     5821 ctttccgtaa aggatcagat gttcttcgcc aagcgccttt ccttcctcat caaagcgggc
     5881 gtccctattc tcgagtcgct ccacatgata agcagccaga cagcgtcgcg ccaaagcgca
     5941 cgcgttatcg gcagcgtgta ccaggacatc tctaacggct cttcccttgc caagagtctc
     6001 ggaaagttcc cccacatctt cggtgacttc ggcatcaaca tcattaagat cggggaatcc
     6061 tccggtacgc tctccacaaa ccttgagtac cttgccgacg agctcaagaa gcgtcactcg
     6121 cttcagggga aagtcgtaag tgccttcatc taccccggca tcatcacgct tgcgaccctt
     6181 ggcatcaccg cattcctcat gctctatctc ttcccgaaga tcatgcctat cttcagctca
     6241 ctccatatgg agcttccgct caccacaaag attgtgatgg ccatctccct cttcctccaa
     6301 gcatgggggc tcctccttat cggcgttatc atcgcggcct gcatcggcat ttctctactc
     6361 aagaagaaga ataagcagtt ccatttcttc ttcgatcagg tagtgctctc tatgccgctc
     6421 ttcggcccct tgatgcgtta ctacaacctt gcaaacggca cgcgcacgct cggcattctc
     6481 cttcagggtg gcgttaccct ctcagaagcg ctgccgctca ccgcagacac caccgcaaac
     6541 ctcgtctata aggccgagta ccgaaagatg gcggaataca tccatcgcgg cgagcgcatg
     6601 tcctccttcc tcgtcacctc agagaacctc ttcccgccca tcatggcgca gcttattgcg
     6661 gtgggcgagc gctcggggaa cctctcaaac acgctcgtct acctctccga actctatgag
     6721 gcggaggtag aggacttcac caagaacctc tcgagcctta tcgagccggc actcatggtc
     6781 atcatgggta tcttggttgg gttcgtggcg atttctatca tcacgcctat ctatggcatc
     6841 acgcaaaatc tccataccta agggattcac cctgcttgag atcctcgtcg tcatggggct
     6901 cgtcctcatg gtggtggcat tcggcctcat cgtatccttc gatgactatc gcggcttcac
     6961 ctttagaaac gaacgcgaca tcgtggtgtc cgtcatacag aaagcgcgcg gccaggcgat
     7021 aaacaatatg tgctttggcg ctgggtgcac cgatgggaag gcacacggcg tgtacttcgg
     7081 tacgccagga gagtacgtgg tattccaagg cacctcgtat gccacgcgcg acgttgcgct
     7141 cgatgaggtg atagcggcgc gcgataacgc atccgtagtt tctggaatga cgagcgtggt
     7201 ctttaatcgt ctgcgcgcgg acacgacgac cgtaggagga acaacgctca ccatcaccca
     7261 gccagggcac acctctacca tcaccgtaga accagagggt cgtgtgtggt ggaccaattg
     7321 atatgatgac gccccgcgga ttctctacgc ttgaaatgct catcgcgatg acggtgctcc
     7381 tgcttgcgtt tactgcgacc acgctccttc ttcccggcat ccaagacagc tctattgata
     7441 cggagattgc cgttgaggca ctcaacatcg ctgagcgcac cttagagaat cagcaggcgc
     7501 tcgcacgcaa ggattttaaa ctcgttaccg gtagttcttc ccaggaaaca attggtggca
     7561 ccgtctatca aaaaacggtg accgcagcgt caactgatta cttcactaag caaatcactg
     7621 cgcttgtcac ctggggcggc atgtatgggc gcgctcaatc gataaccctg aagtcagtcg
     7681 tatcgaactt tgaagaatca attggtggcg atacctgtga ctccatcctt accggtaact
     7741 ggcttcttcc gccgcaaatc acgaatcgca cgctcggtgc acagattatg ggagacggct
     7801 caggactcta tcccatcacc gacctcgatg cgtaccgcaa acgcctctac gtgaccgtaa
     7861 acaacaccgg tacccttccc tcttcgggac cacgcgacgc agggaccgct gcgaacacgt
     7921 cgggcgtggg tacggtgtcg tggggcaatc caaccaacgc gcgtacgagc gataactcat
     7981 acgccactgc ctctctttca ggaactcagg cgacgcagta tataaaggcg acgaactttg
     8041 gcttcactat tccgcaggac gccaccatcg ttggcatcaa ggttgatatt gaacgcaaag
     8101 cgagcagcaa cacgaacacc gtacgcgata acgtggtgcg cctggtacgt gctgacggtt
     8161 cactcggcgg caccaaccaa gcgcacacga cgagctggcc gacctctgac gccgcagact
     8221 cgtatgggag cgcgaccgat ttatggaacg agtcggggtg gagccccgca gcgattaatg
     8281 acgctgactt tggtgcggtt atttctgcta tcgccaacgg cggctcaggc acgcgtaccg
     8341 cgtcagttga tagtgtccgc atcaccgtta cctatacgcg ccagctctac gtactgaacg
     8401 caaccacccc tacaaaccca acgtttgat
//
LOCUS       DAORIN010000017         8207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1541_length_8207_cov_5.128189, whole genome shotgun sequence.
ACCESSION   DAORIN010000017 DAORIN010000000
VERSION     DAORIN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1541_length_8207_cov_5.128189"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PK109_01255"
     CDS             <1..1005
                     /locus_tag="PK109_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24204.1"
                     /translation="VALGTLARTAQKTVTIQTGEGTTTATTTQTISYTELRYVERESG
                     NVYAYDFDKRLLSRISNRTLPGIQEASWSLDGNTAFLRFLSSNDNGGESIDTFALPVT
                     REDGGYFLETGLDQVVVSGSSTVITLLPSSTGSIATSARIDGTNAKTFFSSALSSLRL
                     FPAGTGVAAFTKASAQSDGVGFMVSSGGTFTRILGPLRGLTLLPSPSGKSVLYSYVSG
                     NSVVAGMLDVATRTTTSLPIAALPEKCVWAANETAVYCGVPRSMGTGWPDSWYQGTTS
                     FSDRIWKVDLTARTAILIVDTTAVADTAVDAVSLSIDPKSDVLLFTNKKDGSLWAYDL
                     "
     gene            complement(1002..1736)
                     /locus_tag="PK109_01260"
     CDS             complement(1002..1736)
                     /locus_tag="PK109_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRH24205.1"
                     /translation="MSSLFHNVVYLPIYNLLIALVDVIPGGDVGLAVIGITVIVKLVL
                     LPLSLSAVRTQRAMKLLEPELKELKERYKDDKETQAREMFALYKKNNVKPFASILMLF
                     IQLPILFGLYFVSQHAVLQQVDPTLLYSFISVPETVSPLFLGIFVLTGSSLILALVAA
                     ATQALQAWYAIPVPPKSTSKTPSMQEDFGRAIALQARFIFPLLIGFIAYTSAVLALYF
                     TASNLFMVAQEFVVRKLHPTPKPVTA"
     gene            complement(1733..2065)
                     /locus_tag="PK109_01265"
     CDS             complement(1733..2065)
                     /locus_tag="PK109_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRH24206.1"
                     /translation="MLPRRSRLSRVSFPGPRAGFQASTPHFSAVWGPSTHGGGAAAVV
                     SKKVARRSVDRHLLKRRMLEAMRPFALPDRFLVVYAKGGSHTLPFRALAEELQALLAK
                     TINHSAHS"
     gene            complement(2067..2216)
                     /gene="rpmH"
                     /locus_tag="PK109_01270"
     CDS             complement(2067..2216)
                     /gene="rpmH"
                     /locus_tag="PK109_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004024238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRH24207.1"
                     /translation="MPKRTYQPKKRKRAKTHGFLSRSKTPSGKRIVAARRRKGRKSLT
                     VSSEK"
     gene            2324..3667
                     /gene="dnaA"
                     /locus_tag="PK109_01275"
     CDS             2324..3667
                     /gene="dnaA"
                     /locus_tag="PK109_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRH24208.1"
                     /translation="MNRSDPHELWEYILSQVELSISPANFNTWFRESHIVKIEEGIVY
                     IGVPSQFFRDWYLKKFNALLLKILRSVSYEYRNIEYLIVKDARKPKVEKRERQNQELP
                     LDEFYINKSDNLNPRYTFDTFVVGSFNELAYTAAQAAIKRPGITYNPLFIYGDTGRGK
                     THLIQAIGNQFKKLYPTRKVFYLTSEKFSVEYTDAIGQGTANRFKEKYRQYDLLIMDD
                     VQFLSKKEKTQEELFHLFNALHDNNKQIIFSSDRPPHTIPDIADRVRGRLSSGMTIDI
                     SEPDTESRIAIVRKKAAQHGVTLSDDVIEHVATSVSGSIRELEGIINSLVCHAQVKGV
                     APDLAEVRQSLRSFARPTKSVSVKHVVSKVAEFYGIDEESIYEKTRRREVVRPRQVIM
                     YILREDFSVSYPTIGSKLGGRDHTTVIHSCEKVRREVVDDVELAKEIQDIRGLLV"
     gene            3881..4981
                     /gene="dnaN"
                     /locus_tag="PK109_01280"
     CDS             3881..4981
                     /gene="dnaN"
                     /locus_tag="PK109_01280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRH24209.1"
                     /translation="MNISIDKKELGDAMGVVSRFAERRGATLPVLGGIAIIATKESIR
                     LCATNLETGIDLTITGSVKTPGNIALPATVLKEITSSFSGTGVLTLEQTGDTALISSG
                     SAKSTIKTLSYEDFPTLSVPESSKTSFTLSGTILRNLIQSVASCASPSTVRPELASIL
                     LSAEGGVVKAVATDSFRLAEKKVSVSGKVEPFSILIPAKNALDIAQTLPDDEIKVQAD
                     AHQCSFSFKNGVVVTRLVAASYPDYAQIIPKTSVAEATILKKDFEAGLRRASVFTDAF
                     QKVRLGLDVGKKQLILSARNADVGETSESLPVAASGDSIELSFNHRYLQAPIPLITTE
                     SLTLSAAGIGRPLVIRGQGDTSFLYLVMPMNQ"
     gene            complement(5272..7443)
                     /locus_tag="PK109_01285"
     CDS             complement(5272..7443)
                     /locus_tag="PK109_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRH24210.1"
                     /translation="MKHSKHLKKLSDYLETRHPVARVVFVGFGVLFLAFGATLLYVAL
                     APVPDLDTLSTRKISESTRILDREGTTVLYDLNPDVTRNMVPLSEISPRIQQATLAIE
                     DSGFYYHNGISILAIARAIYVDIATRSLAQGGSTITQQVVKNTLLTNDKSFIRKIHEW
                     VLALKLEQRYSKNQILELYFNNTPYGGTLYGIEAATRAYFGKSAKDVTLAEAAYLSAL
                     PQAPTYFSPYGNNREDLDARKDFVLERMNKLGYINDEEFEAAKAEKVVWSPQNSNSII
                     APHFVFYIEQYLENKYGPTAVTRGLEVQTTLDVDLQHQAEAIVNATALANEKKFNASN
                     AALVAIDPKTGQILSMVGSRDYFDKNIDGNYNITIAERQPGSSFKPFVYAAALLKGYT
                     PNTIIFDLPTQFSTACDQADVFNSVYPCYAPGNYDSKFRGPMTFITALSQSINVPAVK
                     VMYIAGIDNVLDLVSRMGISTLGSAKQYGLSLALGAGEVKLLELTSAYSAFANDGVLN
                     PPVGILKVTDSDGQVLEEYEAHPTMVIDTGVARDISYMLSNNEARFPEYPPNNPFHFE
                     GFDVAAKTGTTNDYRDAWTVGYTPSIAVGVWAGNNDNSPMVKEIAGYIVAPMWHEFME
                     HALQKYPQEFFGEPRAIPDNAPAALRGVYSGNNGEIHDILWWVNKDDPLGGGSSRNDA
                     QARNWEFPLHSLQDAIVPPVVDNDNNPNTNTGINGEEIGGQ"
     gene            complement(7516..7647)
                     /locus_tag="PK109_01290"
     CDS             complement(7516..7647)
                     /locus_tag="PK109_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24211.1"
                     /translation="MDDDEKDLDEPLDLDDEVSDLEGEEAELDSMGMHIEEPKDDTF"
     gene            complement(7735..>8207)
                     /locus_tag="PK109_01295"
     CDS             complement(7735..>8207)
                     /locus_tag="PK109_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRH24212.1"
                     /translation="ILAIDPGFDRLGIAVLEGDASRPTVVWSDCIQPPKGMKEERLSA
                     VEKAIASAILEYAPDAFALETLFFSTNKKTALGVAEARGAALSAAGAAGIPVREYSPG
                     TVKLAVTGNGRADKKAIAVMIPKLVTLTSRKRLDDEYDAIAVGIAALADRFPQR"
BASE COUNT         2011 a         2062 c         2145 g         1989 t
ORIGIN      
        1 gtagcacttg gaacccttgc ccgcacggcg cagaaaacag taactatcca gacgggagag
       61 ggaactacca ccgcaaccac gacccaaact atttcctata ctgaactccg ttatgtggag
      121 cgagagagcg gcaacgtata tgcgtatgac tttgataagc gcctgctgtc ccgcatctca
      181 aaccgcacgc ttcctggcat tcaagaggct tcgtggtctc ttgatggcaa cactgcgttc
      241 ttacgcttcc tctcctcaaa cgataacggc ggggagagca ttgatacgtt tgcgcttcca
      301 gtaactcgtg aggatggtgg ttacttccta gaaacagggc tggatcaggt ggtggtgtcc
      361 ggaagctcca cagttattac gcttcttcca agctcaaccg gatccattgc tacctccgca
      421 cgcattgatg gcacgaacgc gaagaccttc ttctcgtcag cactctcttc gcttcgcctt
      481 ttccctgcag gcaccggcgt tgctgcgttc accaaagcat cggcacagtc ggacggcgtc
      541 gggttcatgg tgagctcggg aggcacgttc acgcgtatcc ttggtccgct tcggggtctc
      601 acccttcttc caagcccttc gggaaaatcg gtgctgtata gctacgtttc tggaaattcc
      661 gtggtcgctg gcatgcttga tgtagcaacg cgcaccacca cctcacttcc tatcgctgca
      721 cttcctgaga agtgtgtctg ggccgcaaat gaaaccgcag tgtactgcgg tgtaccacgc
      781 tctatgggaa ccggctggcc cgactcttgg taccagggca ccacttcatt ctctgaccgc
      841 atctggaagg tagatcttac ggcacgtacc gctattctta tcgttgatac cactgctgtc
      901 gccgacactg cagttgatgc cgtctcactt tcaatagatc cgaagtctga tgttcttctc
      961 tttacgaaca agaaagacgg ttctctgtgg gcgtacgatc tttaggccgt aactggtttc
     1021 ggggtagggt ggagtttacg cacaacgaac tcctgcgcca ccatgaagag gtttgaagcg
     1081 gtgaaataaa gcgccagtac tgctgaggtg taggcgatga atccgatgag aagcgggaat
     1141 ataaagcgtg cctgaagtgc aatggcacga ccaaagtcct cctgcatgga aggagtcttg
     1201 cttgttgatt tcggtggcac cggaatggcg taccacgcct gaagcgcttg ggttgcggcg
     1261 gcaacgagcg cgaggatgag acttgagccg gtgaggacga agatgccgag gaagagtggg
     1321 gacacagttt ccggtacaga gatgaaggag tacagaaggg tagggtctac ctgctgaagc
     1381 accgcgtgct gggatacgaa gtagagaccg aaaaggatag ggagctggat gaagagcatg
     1441 agaatgctcg caaacggctt aacattgttc ttcttataga gcgcaaacat ctcacgcgcc
     1501 tgtgtctcct tatcatcctt atagcgttcc ttgagctcct taagctcagg ctcaagaagt
     1561 ttcatcgcac gctgcgtgcg caccgcagaa agcgagagag gaagaaggac gagcttcacg
     1621 atgacagtga taccaatgac ggcaagacca acgtctccgc cgggaatcac atccacaagc
     1681 gcaatgagga ggttgtagat cggaagatag acgacgttgt gaaagagtga gctcatgaat
     1741 gtgcagaatg attaatagtt ttagcaagca gtgcctggag ctcctccgca agcgcccgga
     1801 agggaagcgt gtggctcccg cccttggcgt agaccacaag gaagcggtct ggaagagcaa
     1861 aggggcgcat cgcttcaagc atacggcgct tcaggaggtg gcgatccaca gaacgacgtg
     1921 ccactttctt agatactacc gcagcagcgc ccccaccgtg agtggaagga ccccatacgg
     1981 ctgaaaaatg aggggttgag gcctgaaaac cggctcgagg ccctgggaag ctaacccgcg
     2041 aaaggcggct tcggcggggc agcatgctac ttctccgagg aaacggtgag ggacttgcgg
     2101 cctttgcggc ggcgggcggc aacaatgcgc ttcccggacg gggtcttgga gcgggaaagg
     2161 aagccgtgcg tctttgcgcg ctttcgcttc tttggctggt aggttcgctt tggcataaat
     2221 tgagactaaa tagttatcaa caggatagta ccaagttctc cactgtccgg caaggaagcg
     2281 gcggactttc acatctagct gtgacgcgta taatacgcca acgatgaacc gttccgatcc
     2341 gcatgaactt tgggaataca tactttcaca ggtcgaactc tccatatctc ctgctaattt
     2401 caacacttgg ttccgtgaaa gccacatcgt caagatagaa gagggaatcg tgtatatagg
     2461 cgttcccagc cagttcttta gggactggta tttgaagaaa ttcaacgcgt tattgctcaa
     2521 aatacttcgt tctgtgtcct acgagtaccg caatatcgag tatttgatag taaaagacgc
     2581 tcgtaaacct aaggtggaga agcgtgagcg ccagaatcag gagcttcccc ttgatgagtt
     2641 ctacattaat aagagcgata accttaaccc gcgctacacc ttcgatacct ttgtcgtggg
     2701 ttccttcaac gagctcgcgt ataccgccgc tcaggccgct ataaagcgcc ctggcatcac
     2761 gtataacccg ctattcatct acggagacac cggacgcggt aaaacccacc ttatccaggc
     2821 aatagggaat caatttaaga agctctaccc aacgcgcaaa gtcttttacc taacgtcaga
     2881 gaagttctcc gtggaataca cggacgccat agggcaggga accgctaatc gctttaagga
     2941 gaagtaccgc cagtacgatc ttttgatcat ggacgacgtc cagttcctct caaagaagga
     3001 aaagacccag gaagagctct tccatctctt taatgcgctc cacgacaaca acaaacaaat
     3061 catcttctca tctgataggc cgccgcacac catccctgac atcgctgatc gtgtccgtgg
     3121 acgcctctca agcggtatga ccatagatat ctctgagcct gatacggaga gccgtatcgc
     3181 cattgtgcgt aagaaggccg ctcagcatgg ggttacgctc tcagacgacg ttatagagca
     3241 cgtcgcaacg tctgtttcag gctctattcg tgagcttgaa ggcattatta actctctcgt
     3301 gtgccacgca caggtaaagg gagtagctcc agaccttgct gaggtacggc agtcgcttcg
     3361 ttccttcgct cgtcctacga aatcagtgtc cgtaaagcat gtagtctcta aagtcgctga
     3421 gttctatggc atcgatgagg aaagtatcta tgaaaagact cgtagaaggg aggtggtacg
     3481 cccacgtcag gtgattatgt acattcttcg tgaggatttc tccgtctcat acccaacgat
     3541 tggttcaaaa cttggtggac gcgaccacac taccgttatt cactcctgtg agaaggtgcg
     3601 tcgtgaggtg gtggatgatg ttgagctagc aaaagagata caggatatcc gcggactgtt
     3661 ggtataacag gggataaact atgtataaga tgccgaacaa ccacaggaca tctcaccaat
     3721 ttccactaac aatgttgggt agggaaaata ctatccgcag ctcttcgttt tcacgcagtg
     3781 ggttttcaac agcttcaaag ataataagta gcgaaaatac tcaacaaata aaaggtggta
     3841 ttagttttcc ccatatccac aatactaata gtatcttcat atgaatattt ctatagataa
     3901 gaaagaacta ggagatgcga tgggggttgt atctcgattc gcagagcgtc gtggcgcaac
     3961 actccctgta cttggcggta tcgctattat tgccacaaaa gaatctattc gtctttgcgc
     4021 caccaatctt gaaactggta ttgatctcac tattaccggg tcagtaaaaa ctcctgggaa
     4081 tatcgcactc cctgcaacag tgctgaaaga aatcacttct tccttctcag gtaccggggt
     4141 tcttactctt gaacagacgg gggatacagc gcttatctct agtggatcag cgaaaagcac
     4201 catcaaaacc ctctcctacg aggattttcc cactctctcc gtaccagagt cttctaaaac
     4261 ctcgtttacc ctctcaggaa ccattctccg caaccttata cagtcggtag cgtcgtgcgc
     4321 gtctccttca acggtacgtc ctgaactagc ttccatactt ctctctgctg agggtggggt
     4381 agtgaaggcg gtagctactg actcattccg tcttgcagaa aagaaagtgt ctgtttcagg
     4441 aaaggtagaa cctttctcta ttcttattcc tgcaaagaac gcgcttgata ttgctcagac
     4501 tcttccagac gatgagatta aagtgcaggc agatgcgcac cagtgctcgt tctcctttaa
     4561 gaatggagtg gtggtgacgc gtcttgtagc agcaagctac cctgactatg cacagattat
     4621 tccaaagacc tcagttgctg aggcgactat tctgaaaaaa gactttgaag cgggactccg
     4681 tcgcgcctca gtgtttactg acgccttcca aaaggtccgt cttggccttg atgtagggaa
     4741 gaagcaactt atcctctctg cacgtaacgc agacgttggt gagacgtctg agagccttcc
     4801 agtagcggct tcaggtgatt ctatagagct ctcattcaat catcgctacc ttcaggcacc
     4861 tattccactc attactactg agagccttac gctctctgct gcaggtattg gacgtccgtt
     4921 ggttatacga gggcaggggg atacttcgtt tctgtattta gtgatgccga tgaatcaata
     4981 atggaaattc ctataatcat tgattacctt cttcttttgg gtgctatatt tgcgctgatt
     5041 tttacttttc tcacgttact agcagcaggc tatttcatta aaaaagcaaa atctgattcg
     5101 gacgcaaatt ttattctcgc cacaagtctc tcggtcatag ctttgtttat aacaattgcc
     5161 agcgttgaaa ttcaaattta tataaacgac aactcctcaa aggaagagag gatttttaga
     5221 gaacaagaac tgaagatact cgaggaaata tcaaaaaaac tggacaaata gctattgacc
     5281 cccaatctcc tcaccattaa ttcctgtatt agtgtttgga ttgttgtcgt tatctactac
     5341 cggaggaact atcgcatcct gaagcgagtg gagagggaac tcccagttac gtgcctgagc
     5401 gtcattgcgt gaggagccac ctcccaacgg atcatcctta ttcacccacc agagaatgtc
     5461 gtgaatctcg ccattgtttc ctgagtacac gccacgaagt gctgcaggag cgttgtctgg
     5521 aatcgcgcga ggctcaccga aaaactcctg aggatacttc tgaagtgcgt gctccataaa
     5581 ctcatgccac ataggggcaa cgatgtagcc ggcaatctct ttcaccatcg ggctgttgtc
     5641 gttgtttccc gcccatacgc cgacagcgat agaaggggtg tagccgacgg tccacgcgtc
     5701 acggtagtcg ttcgtggtac cggtctttgc tgccacatca aagccttcaa agtggaatgg
     5761 gttgtttgga gggtactcag ggaaacgtgc ctcgttattt gaaagcatgt aggagatgtc
     5821 acgagcaacc ccggtatcaa tcaccatcgt tgggtgtgcc tcgtattctt cgagcacctg
     5881 accgtctgag tcggtgacct tgagaatacc tacgggagga ttgaggacac catcgtttgc
     5941 gaatgcgctg tatgcgcttg tgagctcaag gagcttcact tcgccggcac cgagtgcaag
     6001 tgagagaccg tactgcttcg cagaaccaag agtagaaata cccatgcggc tcacgagatc
     6061 gagcacgtta tcaatgcccg cgatgtacat caccttcacc gcaggaacgt taatggactg
     6121 cgagagcgca gtgatgaaag tcatcgggcc ccggaacttg ctgtcatagt ttccaggggc
     6181 atagcaggga tacacgctgt tgaagacgtc tgcctgatcg caggcagtag agaactgggt
     6241 agggaggtcg aagatgatgg tgtttggggt gtagcccttc aagagtgcag cggcatacac
     6301 gaacggtttg aaggaggagc ctggctgacg ctcagcgatg gtgatgttgt agttaccatc
     6361 gatgttctta tcaaagtaat cacgggaccc gaccatcgag agaatctgtc ccgtctttgg
     6421 atcaatcgct acaagtgccg catttgatgc attgaacttc ttttcgtttg cgagcgcggt
     6481 ggcgttcacg atagcttctg cctggtgctg aagatccaca tcaagggtgg tctgtacttc
     6541 aaggccgcgg gtaacagcgg ttgggccgta cttattctca agatactgtt cgatatagaa
     6601 gacgaagtgc ggcgcgatga tggagtttga gttctgcggg ctccacacga ctttctctgc
     6661 ttttgctgcc tcaaactctt cgtcattgat gtagccgagc ttattcatgc gctcaagcac
     6721 gaagtctttg cgtgcatcaa ggtcctcacg gttgtttccg tagggggaga agtaggtagg
     6781 ggcctgagga agagcagaga ggtaggcggc ctcagccaac gtcacatcct ttgcggactt
     6841 tccaaagtac gcgcgtgtcg ctgcttcgat gccgtagagc gtgccaccgt acggggtgtt
     6901 attgaaatag agttcaagaa tctggttctt gctgtagcgc tgctcaagct tgagcgccaa
     6961 gacccactca tgaatcttac ggatgaaact cttatcattg gtgagaagag tgttcttcac
     7021 cacctgctgc gtgatggtgg agccaccctg agcaagggag cgcgtcgcaa tatcaacgta
     7081 gatagcgcgg gcgatagcaa gaatgctgat gccgttgtgg tagtagaagc cggagtcttc
     7141 aatggcgagc gttgcctgct ggatgcgtgg tgagatttct gaaagcggca ccatgttgcg
     7201 ggtgacgtca gggttgaggt catacaagac cgtggtgcct tcgcggtcca gaatgcgggt
     7261 tgattcacta atcttgcggg tagagagcgt atcaaggtca ggtacggggg cgagcgctac
     7321 ataaagaagg gtggcgccga acgccaagaa gaggacaccg aaccctacga aaacgacccg
     7381 ggcgacaggg tgacgggtct caagatagtc agagagcttc ttaaggtgct tggagtgctt
     7441 catattttgg aatcgtagca taaaacgaac gactggccgc cctatgacag gcggccagtc
     7501 gtttgtgaga gtgcgttaga acgtatcgtc cttaggctcc tcgatgtgca ttcccataga
     7561 atcgagttct gcctcttcgc cctcaaggtc cgagacctcg tcgtcgagat ctagcggctc
     7621 atccaaatcc ttttcatcat catccatatc gtctagttaa attattcttt tgcccgaggg
     7681 gcactaaccg ctatatatgt aacagacccc attttatatg ggaaggggac aaccctagcg
     7741 ctggggaaaa cggtctgcga gcgccgctat tcctaccgca atagcgtcat attcgtcatc
     7801 caggcgctta cgcgacgtga gggtgacgag cttcggaatc ataaccgcta tcgctttctt
     7861 atccgcccgg ccattccctg taacggcaag ctttacggtg cccggggagt attcacgcac
     7921 cggaataccg gcagcccctg ctgcagatag tgctgcgcca cgcgcctcgg caacgccgag
     7981 cgcagtcttc ttattggtgc tgaagaacaa cgtctcaagc gcgaacgcat ccggagcgta
     8041 ttcaagaatc gcactcgcta tcgctttctc taccgcagac agacgctcct ccttcattcc
     8101 ttttggtggt tggatacaat cgctccacac taccgtgggg cgcgacgcgt ccccctcaag
     8161 tacggcaatg ccaaggcgat caaaccctgg gtctatcgca agaatac
//
LOCUS       DAORIN010000018         8197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1543_length_8197_cov_4.992999, whole genome shotgun sequence.
ACCESSION   DAORIN010000018 DAORIN010000000
VERSION     DAORIN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1543_length_8197_cov_4.992999"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PK109_01300"
     CDS             complement(<1..1036)
                     /locus_tag="PK109_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24213.1"
                     /translation="MAKRVTEHLFTGGTVMYDTYDSTKTVLGDLIRQQTGAASTDKYA
                     GPTKVAVARPNETSVAVPGLYPHVVRWSDKSGYYITGTVAVSGTAVTGSSTTWLAAGV
                     PVNARIGFGTTDPVAVTAWYNIVAIPSDTSLTLDGSAGTIAGGTSFVIDMNYNVDWVF
                     LADGAATAATRRITCFEYRRGLSIFTWKGFITQTYPTATNHTITGMRAVYAKYTTGTV
                     GVSTATVTGSGTTWSADRINIGSRIGFGSSDPTQISTWYYINAVGSDTSITIQNNVTA
                     SGTGGVASNLSISSGTAYVIEDLRILTTTTNATATNGGLFMSAGMSQDDFTPAGTTIA
                     AAVTTDKVKAV"
     gene            complement(1047..1775)
                     /locus_tag="PK109_01305"
     CDS             complement(1047..1775)
                     /locus_tag="PK109_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24214.1"
                     /translation="MNLFPLYTNNTRIPQVSSNATILANRYSFRLVRRAPVLRAKGEV
                     IHSQVELPPAEPLPPTAVEPQTAPEIVRVQGVDDGKRSFLKLASVVGLGVVAASALPQ
                     KAQAYVMGSAPTSSVVGVKNASNVRVNPATQETLQVLVEGNSVLKKTISLSGSGTVHT
                     PASGKKVRIYNTKFSLDATMTSVSFRFTSGGTDFEKYVAPRTGGLYGTNNHPNYKEGG
                     VDEVVYCSISGTGNVQINIDYLEV"
     gene            complement(1871..2851)
                     /locus_tag="PK109_01310"
     CDS             complement(1871..2851)
                     /locus_tag="PK109_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRH24215.1"
                     /translation="MAWGIRLRNKITNDPFLAGRLRMTFYYLAFCTVVFWSGSFLVDH
                     YAHTVLLQAFADSAARSPEEVMALIDQSRMKIRIFYVIVFSLGAYVLMGEALRPVREA
                     LESERRFIANVSHELRTPLTLAKAETEVLLKKPELLDLPRATAALRKNLTEIDHVSRI
                     IQFLLVLSDFNGQKGTTTKQQVPLSQIVRAAEAQSARLLNEKKVRLLTAVPEGDTRVR
                     GNAIALEKLLLNLVRNAAIYSPEGSTIEVSVERTGSGKLLLAVRDYGIGIPASEHKHI
                     FKPFYRGMNASPGGTGLGLAIVQEVVRLHNARIRVDSVEGKGSTFTIEFS"
     gene            complement(2823..3470)
                     /locus_tag="PK109_01315"
     CDS             complement(2823..3470)
                     /locus_tag="PK109_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRH24216.1"
                     /translation="MSSASRRSSSRGSSTRIIEWTSSTPRAPRERLEYSGSLYDLIIL
                     DLMLPDGDGTDVCSGIRASGVQTPVLVLTARDSTEDKVALLDRGADDFLTKPFDFDEL
                     LARSRALARRANDGPAATIAISDVTIVPERFEVRRKNKILPLTAREFELLAYLVRNRG
                     KVVKREELLARVWGQTQATTNVVDVHIRNLRKKLDEPFETKLIQTIHGVGYTIAE"
     gene            complement(3522..3770)
                     /locus_tag="PK109_01320"
     CDS             complement(3522..3770)
                     /locus_tag="PK109_01320"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRH24217.1"
                     /translation="MEERKPIPVTIPATALPAFANATKVTVSDDSVIVQFAYVRPGGT
                     NGQLISEVVLTPKHAINFSRSLDATIKKHFTRHLEAEP"
     gene            complement(3772..3966)
                     /locus_tag="PK109_01325"
     CDS             complement(3772..3966)
                     /locus_tag="PK109_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24218.1"
                     /translation="MIKLIIWIVIGLLALSFFGISLRAIAGSPTNQDNIQFLLTTLQQ
                     GWDYIVAWVADLLSVFDFAQ"
     gene            4025..4636
                     /locus_tag="PK109_01330"
     CDS             4025..4636
                     /locus_tag="PK109_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRH24219.1"
                     /translation="MHDIAAALAHFKKVDPILYKAAKKVEAQLTARKPLRGSTALFNS
                     LCESVVSQQLSVKASDTIWKRVQEVCGGTVSPDALIKASLPRLRKAGLSAAKVKTLKE
                     LAKAVQGGLNLGALRKNTPEEATEALVAVWGIGPWTAEMFLMFGLSHPDIFSPRDLGL
                     VRSMETLYALKKDSSFAKYEKIAAAWSPHRTIACRVLWRVRDM"
     gene            complement(4672..5151)
                     /locus_tag="PK109_01335"
     CDS             complement(4672..5151)
                     /locus_tag="PK109_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRH24220.1"
                     /translation="MTEGVEKESAPKLFTLRQATEEDLFFLYRVSTEAMRPVSELSNA
                     PARSEEERLTEYKKKFVPETIHIIQHNGEDIGRLRIVRSSDSIYVGGIQILPEHQGKG
                     IGTALFTELIEESNRMRIPILLEVHDVNTPAIAFYKRLGFQEGEKVKDQVVMRYLPA"
     gene            complement(5200..6540)
                     /locus_tag="PK109_01340"
     CDS             complement(5200..6540)
                     /locus_tag="PK109_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRH24221.1"
                     /translation="MIRFALVSFLFLIILFSPVSVPLQIHADTVDEIKAQIADINRQR
                     AALDAEIAGYQKQLNVLSGQKQTLQGAIQTIDVSRNKTAAQIKEIQQKIAAATLTLNK
                     LGIEIHEKEESISLDRSAVAASLRAIDSTDDSTLIEQILGATDLADAWVTIDNLSTLN
                     EALSTHRAALAEAKTVLASQHQAVASTKTELSSANVDLSNQKKALDVQKAAKATLLSQ
                     TQSTEAAYQELIAKKRAQQAQFEAALSQLEDSLTTVVDSTQIAAAGSGVLKWPFSDAF
                     MAGCAAKQGALGNIWCVTQYFGNTSFSTANPQIYNGSGHNAIDIGVPTGTPVEASLAG
                     TVMDTGNTDSVPGCYSFGKWVVIKHSNGLATLYAHLSSIQVSAGQSVSTGSVIGYSGM
                     TGYATGPHLHFGVYAAQGIQILTLAQYRGATSPCANAKMPVAPKDAYLNPMSYL"
     gene            complement(6544..7848)
                     /locus_tag="PK109_01345"
     CDS             complement(6544..7848)
                     /locus_tag="PK109_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRH24222.1"
                     /translation="MSVVTRIPPSPTGHLHIGTARTALFNYLYAKSQGGKIIFRSEDT
                     DRARSKPEFEKEIIDGLEWLGLSWDSFSRQSEHTARYSELLHTLVASDKAYVSKEPAK
                     DDPSREVEVVRLRNPGTEVIFDDAIRGPINTHTGDLGDFVIARSLDDALYHFAVVVDD
                     MDGGVTHVIRGEDHISNTARQILIQEALGAPRPIYAHLPLILASDRSKMSKRHGAVSL
                     LEYRQEGFLPEAIVNYLALLGWNPKDEREDFSLAELVDIFSLANVHRSGAVFDMVKFK
                     NVNQRWMRKLSDEEFIARGALADNDPKVMKAAPLLKERATTFGEARALLSGEFACLFV
                     TPTPVKETLIAKEPEDKAGFTKTALTEVMALIHTIPEGASVEDIKGIIMPYADKEGRG
                     AVLWPLRYALSGAEKSPDPFSLVFILGKAESLSRIQKALAIL"
     gene            complement(7850..8164)
                     /locus_tag="PK109_01350"
     CDS             complement(7850..8164)
                     /locus_tag="PK109_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRH24223.1"
                     /translation="MTTVAEIALWKKPAILIPIPESVSHDQRTNAYAYAHTGAAVVIE
                     EQNLTSHLLVSEVRRILGDAALRTSMSTKSASFANADAGRIIAEELITLGLSHEPPAP
                     TV"
BASE COUNT         1807 a         2103 c         2280 g         2007 t
ORIGIN      
        1 acactgcttt tactttgtca gtggtcaccg ctgcagcaat agtggtgccc gcaggggtaa
       61 agtcatcctg cgacatgcca gcggacatga agagtccgcc gttggtggct gtagcgtttg
      121 ttgtggtagt aagaatgcgc aggtcctcga tgacgtatgc ggtgccggag gatattgaga
      181 ggttcgatgc gacgccgccg gtaccagaag cggtgacgtt gttttgaata gtgattgagg
      241 tgtcggaccc tacggcattg atgtaatacc aggtagatat ttgggtgggg tcggaggagc
      301 caaatccgat gcgggagcct atgttgatgc ggtcagcgct ccaggtggta ccgctccccg
      361 ttaccgttgc ggtggaaact cccacggtgc cggtcgtgta ctttgcgtac acggcgcgca
      421 taccggtaat cgtgtggttc gtggcagtcg gatacgtctg cgtaatgaat cctttccagg
      481 tgaagattga taacccgcgg cgatattcaa agcaggtgat acgtcttgtt gcggcagttg
      541 cggcgccgtc ggctagaaat acccagtcaa cgttgtaatt catatcaata acaaacgagg
      601 tgccgcccgc tatggtgccg gcagatccgt cgagcgtcag tgaggtgtct gaagggatgg
      661 ccacgatgtt gtaccaggcc gttaccgcta ccgggtcggt agtaccgaag cctatgcgtg
      721 cattcactgg cacgcccgca gcaagccatg tggtgctgga cccggtgacg gcggtgcctg
      781 ataccgctac ggtgcccgtt atgtaatagc ctgatttatc cgaccagcgc acgacgtggg
      841 ggtagaggcc tggaaccgct accgacgttt cgttcggtcg ggcaacagcc acctttgtcg
      901 gcccggcgta tttgtcagtg gatgccgcgc ctgtttgttg gcggatgagg tcgccgagca
      961 cagttttcgt cgagtcgtac gtgtcgtaca tgacggtgcc tccagtgaac agatgctcgg
     1021 tgactcgttt tgccatagta ataagattac acttctaaat aatcaatgtt tatctgcacg
     1081 ttgccggtgc cgcttatcga gcagtacacc acctcatcca ccccaccttc cttatagttt
     1141 ggatggttgt tcgtgccata gaggcctccg gtacgaggtg ctacatactt ctcgaaatcg
     1201 gtgccacctg aggtaaagcg gaaggaaacg ctcgtcatcg tggcatcaag cgagaacttt
     1261 gtgttataga tgcgtacttt ttttcctgaa gcaggggtat gcacggtccc ggatcctgag
     1321 agactaatcg tcttctttaa taccgagtta ccttcaacaa gtacctgaag agtttcttga
     1381 gttgcagggt ttacccgtac attcgaagca ttctttacac ctaccacact cgaagttggt
     1441 gcgctcccca tcacgtacgc ctgtgctttt tgcggtaggg ccgacgcagc tacaacgccg
     1501 agtccaacga cgctcgcaag cttgaggaaa ctgcgcttcc cgtcatccac tccctgtacc
     1561 cgtacaatct ccggcgcggt ctgaggctca acggcggtag gcgggagcgg ctcggctgga
     1621 gggagttcta cttgtgagtg aattacttcc cctttggcgc gtagaacagg cgctcggcgc
     1681 acaagacgga acgagtaacg attcgctagt atcgtcgcgt tagaagatac ctgcggtatc
     1741 ctcgtgttgt tcgtatataa ggggaataga ttcatccatt gccgaagtta gtactcgggc
     1801 actttgaagt atacgtgatg aaattaatga aatattaata aatgcttata tcgaggtggg
     1861 gatagtaacg ctacgaaaat tcaatagtaa aggtcgagcc cttgccctca acgctatcga
     1921 cgcgtattcg tgcattatgg aggcgtacaa cttcctgaac gatagcaagt ccgagcccgg
     1981 tccctccggg gctggcgttc atcccgcggt agaacggctt gaatatgtgc ttatgctcgg
     2041 atgctggtat gccgatgccg taatctctca ctgcaaggag gagcttcccg cttcctgtcc
     2101 tttcgaccga tacctcgata gtgcttcctt ctgggctgta tatcgccgca tttctcacaa
     2161 ggtttaacag cagcttctcg agggcgattg cgttgccacg tacgcgcgta tcgccttccg
     2221 gtaccgccgt taggagccgg accttcttct cgttcaggag acgcgcggac tgggcttcgg
     2281 ccgcacggac tatctgcgaa agcgggactt gctgtttggt cgtcgttccc ttctgtccgt
     2341 taaagtctga gagtacgagc aggaactgga tgatgcgtga cacgtgatct atctccgtca
     2401 gatttttccg taacgccgcg gtcgcacgcg gtaggtcaag gagttccggc tttttgagaa
     2461 gcacttccgt ctccgcttta gcgagcgtga gcggcgtgcg cagctcgtgc gagacattgg
     2521 cgatgaaacg ccgctcggac tcaagcgctt cgcgtacggg acggagcgct tctcccatta
     2581 ggacataggc accgagtgag aacactatga cgtaaaagat gcggattttc attcgcgact
     2641 ggtctatgag cgccatgact tcttcgggag acctagctgc ggaatccgcg aaggcctgca
     2701 gcaacaccgt gtgcgcatag tgatcgacca agaaactccc actccagaac accaccgtgc
     2761 agaatgcaag gtagtaaaaa gtcatccgga gccggcccgc aagaaacggg tcgttcgtga
     2821 tcttattccg caatcgtata ccccacgcca tggatggtct gtatgagctt cgtttcgaac
     2881 ggctcgtcga gtttcttccg aagattgcgg atatgtacgt ctactacgtt cgtggtggcc
     2941 tgcgtctggc cccagaccct agcaagtagt tcctcgcgct tcactacctt gccgcgattg
     3001 cgcacgagat atgcaaggag ctcgaactcg cgcgccgtga gaggcagtat cttattcttg
     3061 cggcgcacct cgaaccgctc cggcactatg gtgacgtcgc ttatggcgat ggtagcggcc
     3121 ggcccgtcgt ttgcgcggcg cgcaagcgcc cggcttcggg ccagaagctc atcgaaatcg
     3181 aatggcttgg tgaggaaatc gtctgcgccg cggtcaagca gcgccacctt gtcttcggtt
     3241 gagtcccgag cggtcaagac taatacgggc gtctgcacgc cggaagcgcg tatgccggag
     3301 cagacatctg ttccgtcccc gtccgggagc atgaggtcga gtatgatgag gtcatagagg
     3361 ctgcctgaat attcgagccg ctcgcgcggt gctcgcggtg tggaagaggt ccactcgatg
     3421 attctcgtac tcgagcccct gcttgaggac ctgcgcgagg cggagctcat cttctactat
     3481 cgctatcttc atccgaagga gtgtagcacg ggtgccctcg gctatggctc cgcttccaaa
     3541 tgacgagtga aatgcttctt tatcgtcgcg tctaatgagc gactgaagtt tatggcgtgc
     3601 ttaggggtca acaccacttc agaaatgagt tggccattcg tgcctcccgg acgcacatac
     3661 gcaaactgca caatgaccga gtcatcagag acagtaacct tggtggcgtt tgcaaacgcc
     3721 ggaagtgcgg tggcggggat ggtaaccgga ataggtttgc gctcctccat gctactgcgc
     3781 aaaatcaaac acgctcagaa ggtcagcgac ccacgcgacg atatagtccc acccttgctg
     3841 aagggtcgta agcaggaact ggatgttgtc ctgattcgtt ggggatccag cgatagcgcg
     3901 gagcgatata ccaaagaacg agagggccag aaggccaata actatccaga tgatgagctt
     3961 aatcatagag ctagtataac cggccctatg agggccctag agcgttcgtg ggtataatgc
     4021 gcccatgcat gatattgccg ctgcgttggc tcactttaag aaggtggacc ctatcctcta
     4081 taaggcggcg aagaaggtag aggcgcagct cactgcccgt aaacccctac gaggttctac
     4141 tgccctcttt aatagccttt gtgagtcggt ggtgagtcaa cagctctccg taaaggcatc
     4201 agacactata tggaaacgag tacaggaggt gtgtggcggc actgtctcac cagacgctct
     4261 cataaaggca tcgctccccc gcttaaggaa agcaggcctg tctgcggcga aggtcaaaac
     4321 cctaaaagag ctcgctaaag cagtacaagg aggactcaac cttggagcgc tgagaaaaaa
     4381 tactcctgaa gaagcaaccg aggcgctcgt tgcggtatgg ggcattggtc cgtggaccgc
     4441 ggagatgttc cttatgttcg gactctctca tcccgacatc ttttctcccc gcgacttagg
     4501 actcgtccgt tctatggaaa cgttgtatgc gttaaagaag gatagttcgt ttgcaaagta
     4561 cgagaaaata gcagcagcat ggagtcctca ccgaacgata gcgtgcagag tgctttggag
     4621 ggtgcgtgat atgtaggttt actggcggtc tgggacgaag ttggaactct attaagcagg
     4681 tagatacctc atcacgacct gatccttgac cttctctcct tcttggaatc caagtctctt
     4741 atagaacgcg attgccgggg tgttcacatc atgcacttca agtagtatgg gaatgcgcat
     4801 ccgattcgat tcctcaatca gctcagtaaa caatgctgtt ccgatacctt taccttggtg
     4861 ttcagggagt atttggatac ctcccacata aatagagtcg gacgaacgca ctatccgaag
     4921 cctacctata tcttctccat tatgttggat tatgtgtatc gtttctggga cgaatttctt
     4981 tttgtattcg gtaagtctct cttcctcgga tcttgcaggc gcattggata gttccgaaac
     5041 aggtctcatt gcctcagtag aaacgcggta gagaaaaaat agatcttctt ccgttgcctg
     5101 acggagcgtg aatagttttg gtgccgactc tttttctact ccttcggtca tgggtctatt
     5161 agaacatgag aatacctgct ggcgggctag gattcgaact tacagatacg acatcgggtt
     5221 aagatacgca tccttcggcg ctaccggcat cttcgcgttc gcacatggac tcgtggcacc
     5281 ccggtactgg gcaagcgtga gaatctgaat accctgcgca gcatatacgc caaagtggag
     5341 gtgaggaccg gtagcgtagc cagtcatgcc tgagtatccg ataacagagc cggtagatac
     5401 tgactgccct gcactcacct gtatagacga aaggtgggcg tagagggtgg caaggccgtt
     5461 cgagtgcttt atcactaccc acttaccaaa cgaatagcag ccaggaaccg agtcagtgtt
     5521 accagtatcc ataacggttc cggcaaggga cgcttctacc ggagtcccgg tgggtacgcc
     5581 gatatctatc gcgttatgtc ccgaaccgtt atagatctgg ggattggccg tagagaaaga
     5641 agtgttcccg aagtactgcg tgacgcacca aatattcccg agtgctccct gcttagcggc
     5701 gcaccctgcc atgaaagcgt ctgagaatgg ccacttcaac acacctgacc ctgctgcggc
     5761 aatttgagtg gagtcgacca ccgtagtgag actgtcttca agctgtgaga gcgctgcttc
     5821 aaactgtgcc tgctgcgcac gcttcttggc gatgagttct tgatacgccg cctcagtcga
     5881 ttgcgtctga gaaagaagtg ttgcttttgc tgccttctgt acgtcgagcg ccttcttctg
     5941 attcgaaaga tcaacgtttg cgcttgaaag ctcagtcttt gtggatgcta cggcctgatg
     6001 ctggctcgcg agcactgtct ttgcttcagc gagtgcggcg cgatgcgttg aaagcgcttc
     6061 gtttaaggtg gagaggttat caatggtgac ccacgcgtca gcgagatcgg ttgcaccgag
     6121 tatttgctcg ataagagtag agtcgtccgt gctatctatg gcacgaagtg aggcagctac
     6181 cgctgaacga tcaagagaaa tagattcttc tttctcgtgt atctcaatac ctagtttatt
     6241 cagcgtaagg gtggcagccg ctatcttctg ctgaatctct tttatctgcg ctgccgtctt
     6301 atttcgtgag acatcaatag tctgaatcgc tccctgtaag gtctgtttct gacccgagag
     6361 cacgttcagc tgtttctggt atcccgcaat ctcagcgtct aacgccgcac gctggcggtt
     6421 aatgtccgct atctgtgcct ttatctcatc aacggtatcg gcgtgtatct ggagcggtac
     6481 cgacaccggc gagaaaagaa tgataaggaa aaggaacgat acaagagcga aacgaatcat
     6541 tagttagagt atagcaagtg ctttttgtat gcgggacagc gattccgctt tacccaatat
     6601 aaagacgagg ctaaacggat ctggtgactt ctctgctcct gaaagagcgt agcgtagtgg
     6661 ccagagcact gctcctctcc cctccttgtc cgcgtacggc atgatgatgc ctttaatgtc
     6721 ttctaccgac gcgccctctg gaatggtgtg tatgagcgcc ataacttcgg taagggcggt
     6781 tttcgtgaat cctgctttat cctcaggctc tttcgctata agggtttcct ttaccggcgt
     6841 tggcgtgaca aaaagacagg caaactctcc tgaaagaagt gcgcgtgctt ctccaaaggt
     6901 cgtcgcgcgc tccttgagaa gcggtgccgc cttcatcacc tttgggtcgt tatcggcaag
     6961 tgcgccgcgt gcaataaact cctcgtcgga gagtttacgc atccagcgct ggtttacgtt
     7021 cttaaacttc accatgtcaa agaccgctcc cgacctatgc acatttgcaa gactaaatat
     7081 gtccacaagc tcggcaagag agaaatcctc acgctcatct ttcgggttcc agccaagaag
     7141 tgcgagatag ttcacgatag cttcggggag gaacccttcc tggcggtatt caagcaagga
     7201 gaccgcacca tgacgcttgc tcatctttga gcggtcagac gccaagataa gcgggaggtg
     7261 tgcgtagatc gggcgtggtg ctccgagcgc ttcctggata agtatttggc gggcggtgtt
     7321 tgagatatgg tcttctccac gaataacgtg ggtgacgcca ccgtccatgt catccaccac
     7381 tacggcaaag tgatagagcg catcatccag ggaacgtgca atcacaaagt cacccagatc
     7441 tcccgtgtgc gtattgatag gtccgcgaat agcgtcgtca aatatcactt cggtccccgg
     7501 gtttctgagg cgcactactt ccacttctct tgaaggatca tcctttgctg gttccttgga
     7561 tacatacgct ttatcacttg ctacgagtgt gtgcagaagc tcgctatagc gggcggtgtg
     7621 ctcactctga cgtgagaatg agtcccagga aagaccgagc cattcaaggc catcaattat
     7681 ttctttttca aactctggct tgctacgagc acggtccgtg tcttcgctgc ggaagataat
     7741 cttccctccc tggctcttcg catagaggta gttaaagagt gcggtacgtg ccgtaccgat
     7801 gtgaaggtgg ccagtaggcg acggtggaat gcgggtgacg acagacatat tagaccgtag
     7861 gagcgggagg ctcgtgggaa agaccgaggg ttataagctc ttctgcgatg atgcgtccgg
     7921 cgtcggcgtt tgcaaacgaa gcactcttcg tgctcataga agtgcgtagt gcagcgtcgc
     7981 ctaggatgcg gcgtacttca gacacgagaa ggtgcgaggt gaggttctgt tcttcaatta
     8041 ctaccgcagc acctgtgtgc gcatacgcat aggcgttggt gcgctggtca tgactcaccg
     8101 actccggaat aggaatgagg atagccggct tcttccaaag ggcgatttct gcaacggtgg
     8161 tcatgggaac ctcctcctgt gtgatgggtc aggcacc
//
LOCUS       DAORIN010000019         7513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1750_length_7513_cov_4.823947, whole genome shotgun sequence.
ACCESSION   DAORIN010000019 DAORIN010000000
VERSION     DAORIN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1750_length_7513_cov_4.823947"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PK109_01355"
     CDS             <1..390
                     /locus_tag="PK109_01355"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF019929.2"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRH24224.1"
                     /translation="KLPISRLQRDLSDSTTERNFGVSFAHSLIGYESLLTGLSRIEPN
                     AGAMAEALAAHPEVLAEAVQTVLRREGQIGAYEKLKDLTRGRAVTLDELREFIKSLII
                     PEAAKKGLLALTPGTYIGIAEKLAKGK"
     gene            complement(435..668)
                     /locus_tag="PK109_01360"
     CDS             complement(435..668)
                     /locus_tag="PK109_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24225.1"
                     /translation="MGPEHMPKPVPTITIDEWHNFCSGSVDPKSEKVQEWIKQKGITM
                     DSGLVTIDFIDDDGTTKRQKMVTDAHDFGTMAE"
     gene            complement(744..2324)
                     /locus_tag="PK109_01365"
     CDS             complement(744..2324)
                     /locus_tag="PK109_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24226.1"
                     /translation="MEKKNALLMAYDKAGIADFARGLMGLGYNIYGSKGTVAALAAEG
                     IAATDIATIVGDPILGHRVVSLSREIFAGILARDIEEDRAELKAAGITWFDLVCVDFY
                     PLQQELADPAHTKESVIEKTDIGGPTAVRAAAKAGRVVVCDPGDRERVLAWLSGGRPD
                     EEAFLDELAAKAEALVSRYVLASAEGRSNGGYAGLIGKEVAPAKYGENAYQAPAALYS
                     GETTDPLALDRFTVIEGTAPSYNNWCDIDRLLQTATHIAAAYDVNRGKTPNIAVGAKH
                     GNACGAAQGENPEEVLKNMLAGDPLSIFGGLVLVNFPIDDELSEILAGNMLDGIIAPS
                     FSEGARDRLRRKGDRCRFIENSALLKLSKDTLDTAARFRYVRGGFLRQPNYTFVMDLK
                     HAELVKHGTATRQQEDDMLLAWAVGATSNSNTIALVKNGMLLGNGVGQQDRVGAANLG
                     LVRATRSGHDVTGAVAYSDSFFPFPDAPEVLFDAGVTAILSTSGSKNDQLSIDLAASK
                     NLALYLIPDSLGRGFFGH"
     gene            complement(2346..3209)
                     /locus_tag="PK109_01370"
     CDS             complement(2346..3209)
                     /locus_tag="PK109_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRH24227.1"
                     /translation="MKHPLLALVVIGVIALAGIGYGWYSSIRSLDQVRTELATATTTI
                     AERDVALDAVRTEYAVLEDAYETEKERNDAFDEKIQDISGTVGKLDKLSKIDPELLQK
                     YSKVFFLSDNYVPAALTQIPEAWVADDKDEYFEKEAYPFLEDLLTDAAEDDIDLKVTS
                     AYRSFGTQAALKSSYTVRYGSGANAFSADQGYSEHQLGTALDFTTKEIGNGLTTSFAS
                     TEAGEWLSENAYRYGFILSYPSGNTYYQYEPWHWRFVGRDLARELHKDGKDFYDLEQR
                     EIDPYLIDLFD"
     gene            3256..3876
                     /locus_tag="PK109_01375"
     CDS             3256..3876
                     /locus_tag="PK109_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRH24228.1"
                     /translation="MSASPERAFNHQAHTADGETLKGVEALTRAELSAMNRKLMTIAL
                     SLTDKHVAKAKDLVQETFERALKSKHTFQEGGNLEAWLATILRNQYYSSNRRAKFWDT
                     EDPETRMREMSVSAEQDGEIDSKQIDALLEKLPERFRIALQTVRDGGDYEEVGHRVGL
                     KPESLKSLMNRARKAFAKVLYDAGYIDSDDELVERFLDKEVKKRKK"
     gene            complement(3883..4509)
                     /locus_tag="PK109_01380"
     CDS             complement(3883..4509)
                     /locus_tag="PK109_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24229.1"
                     /translation="MTGFTASERALFKKLSTPAKIQDFLDSLPFNYEKKGETSYSPLM
                     VIREKKAHCLEGALLAYAALHEAGEKPLLMNLRTLPMDDDHAVVLYRRNGYWGALSKT
                     NHAVLRFRDPIYSSVRELAASYFHEYFMNASGLKTLRAYSKPMSLSRFGTTWIESEEP
                     LWHIAHALRDAPHLPLVPEENRKYLRHASKIERDAGHIVEWQRHNGKT"
     gene            4587..5771
                     /locus_tag="PK109_01385"
     CDS             4587..5771
                     /locus_tag="PK109_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRH24230.1"
                     /translation="MNRSFTLFALTALIGATALIPTSARAAELEVSGWIPYWAVSAGV
                     REAGKHLDELSVIHPFSYSVNTDGKPKDLANMGKSSWQRLIRNAKADGVAVIPTIMWS
                     DTNSIHRILSDEKLRAAHVKAIADLVKKEKYDGIDIDYEGKLAKTRPYYSLFLKELDS
                     ALGAKTLSCTIEARTPPESLYTTIPATLEYANDFGQINKYCDRVTLMTYDQQRADLKL
                     NSARSGAPYYPIADLEWVKKVVALASKSIDKNKLVIGVATYGREVEVTVSPNWFQAYR
                     QISSVSDEYARDTADKHDADLFRNGAGELSYSYAPDKATQKLLASVSLPKSTRPRDEA
                     AARALAYADKTGNTVKIRTVWWSDAKAIEEKADLARALGVKGIAIFKIDGGADEDLWD
                     SF"
     gene            5968..6600
                     /locus_tag="PK109_01390"
     CDS             5968..6600
                     /locus_tag="PK109_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24231.1"
                     /translation="MAALSNVSFVDTEVSLRTDYMDNVVSYAPGSGTPSQNPSGMVSG
                     INVRYLFPVGRFRLGAEAYVLGGHLEDEVDDGFYIDEGTVIDLTYGLDVIAGVVLGSN
                     VLVYAKGGVSYASAKVTKNCPPPVNAHFGYCSTRGPFDHETNVLWQGQNYGAGISVRL
                     GRFNLTGDYQHNDFDALSGVSGQNALGQDVTYNNMALAGDQYGVRIGYNF"
     gene            6694..7122
                     /locus_tag="PK109_01395"
     CDS             6694..7122
                     /locus_tag="PK109_01395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HRH24232.1"
                     /translation="MQKRKKKFEPGNFNLLVKRSTSGLGLFAGEPIKKGSCIIEYKGR
                     TITEAEAYTSRSKYLFEINSKKTIDGRPKWNKAGYINHSCVPNAEPEIRTARVFIMAK
                     KNIKEGEEITYDYGDEYFNDYLAPGGCRCPKHRGKKTAKR"
     gene            complement(7112..>7513)
                     /locus_tag="PK109_01400"
     CDS             complement(7112..>7513)
                     /locus_tag="PK109_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRH24233.1"
                     /translation="KREQSEGAVTDITYKQSFLIGLAQAVAIIPGVSRSGASVMGGLM
                     LGIPRTAILVFSFLLAVPTLLAATAYDIMKNPGVFDGGNVGLLLTGGLAAFVVALIVI
                     KAALGFVRKFTFVPFAIYRILLALVFWFLIF"
BASE COUNT         1842 a         1952 c         1997 g         1722 t
ORIGIN      
        1 aagctcccga tatcacgcct gcagcgcgac ctctccgact ccacgacaga acgtaacttc
       61 ggtgtatcat tcgcacactc cttgatagga tacgagtctc ttctcaccgg tctttcccgc
      121 atagaaccaa acgcaggcgc gatggcagag gcgcttgccg ctcatcccga agtccttgct
      181 gaggcggtgc agaccgtact tcgtcgtgaa ggacagattg gtgcatacga aaaactcaaa
      241 gatctcaccc gaggacgcgc ggtgacgctt gatgagcttc gggaattcat caagagcctc
      301 atcattcccg aagcagcaaa gaaaggactt cttgccctta cgcccggcac gtacataggc
      361 attgcggaga agttagcgaa agggaaataa aaacagcgct cgaacgagcg ctgttttttg
      421 tgaggaggaa tatattattc tgccatcgta ccaaagtcgt gcgcgtcagt aaccattttt
      481 tgtcgcttgg tagttccgtc gtcatcgata aaatcaatcg taacgagccc gctatccata
      541 gttatacctt tttgttttat ccactcctgc actttttcgc ttttagggtc aacgcttccg
      601 ctgcagaaat tgtgccactc atcaatagtg atagttggca ctggttttgg catatgctca
      661 ggtcccataa tcgtcagtta ttttcgtata atttctagat ctaagatatc ttcccaccgc
      721 gtcttgttct gtcaatgtct cttctagtgt ccaaagaatc cgcgtccgag cgaatcaggt
      781 ataagataga gcgctaagtt ctttgatgca gccaaatcaa tagagagttg atcattcttc
      841 gagcctgaag ttgaaagtat ggcagtcacc cctgcgtcaa acagcacctc aggagcgtcc
      901 gggaacggga agaaggaatc gctatacgcg acagcgcctg tgacatcgtg acctgagcgt
      961 gttgctcgaa ccagtccgag gttcgcagca cccacacgat cttgctgacc aaccccattt
     1021 ccaagaagca tgccgttctt cacgagtgca atagtgtttg agtttgaggt tgctcccacg
     1081 gcccacgcaa ggagcatgtc gtcttcctgc tgtctggtgg ccgttccatg ctttacgagc
     1141 tcagcatgct tcaagtccat gacaaaggtg tagttaggct gacggaggaa accgccacgc
     1201 acatagcgga agcgagctgc tgtatcaagc gtatccttgg aaagttttaa gagcgcgctg
     1261 ttttcaatga agcggcaacg atctccttta cggcggagtc tgtcgcgcgc tccttcagag
     1321 aacgaaggag cgatgatgcc atcgagcatg ttacccgcaa gaatctctga gagctcgtca
     1381 tcgataggga agttcacgag caccagtccg ccgaatatgg aaagcgggtc cccggcgagc
     1441 atattcttga gcacttcctc ggggttttct ccttgtgccg caccacatgc gttcccgtgc
     1501 tttgcgccga cagcgatgtt cggcgtctta ccgcgattca cgtcatacgc tgcagcgatg
     1561 tgggttgccg tctggagaag gcgatctata tcgcaccagt tgttatacga aggagcggtc
     1621 ccttcaatga cggtaaagcg atcaagagcg agggggtcgg tagtttctcc tgaatagagt
     1681 gctgccggtg cctggtatgc gttctcgccg tactttgctg gagccacttc ttttccgatg
     1741 agaccggcat acccgccatt gctacgtcct tcagcggacg caagcacata ccgggacacc
     1801 agtgcctctg cttttgctgc aagctcatca aggaacgcct cttcgtccgg gcgtccgcct
     1861 gagagccagg ccaacacacg ctcacggtct ccagggtcgc atacgaccac acgtcccgcc
     1921 ttagcggccg cgcgcactgc agtgggcccc ccgatgtccg tcttctcgat aacagactcc
     1981 ttggtgtgtg cgggatcagc gagttcctgt tggagcggat agaagtcaac gcatactaaa
     2041 tcaaaccagg tgatgccagc agctttcagt tccgcacgat cttcttctat atcgcgggca
     2101 agaataccgg cgaatatctc tcgagaaaga gacacgacgc ggtgaccgag tatcgggtca
     2161 cctacaatcg tcgcgatatc cgtagcggca atgccttctg cggcgagcgc cgcaaccgtg
     2221 ccctttgagc catagatgtt gtatccaaga cccatgaggc cacgcgcaaa gtctgcaata
     2281 cctgccttgt cataggccat caagagagcg tttttctttt ccatacccgt attctactct
     2341 atcgcttaat caaagaggtc gatgagatag ggatcgattt ctcgctgctc gaggtcatag
     2401 aagtccttgc catctttatg gagctcacgc gccaagtcgc gtccgacgaa gcgccaatgc
     2461 catggctcgt actggtagta ggtgttaccg gaagggtagg agaggatgaa gccatagcga
     2521 taggcgttct ccgaaagcca ttcacccgcc tcggttgaag caaatgaggt ggtgaggccg
     2581 ttcccaatct cttttgtcgt gaaatcaagc gcggtaccga gctgatgctc tgagtatccc
     2641 tgatctgcag aaaaggcgtt tgcgcctgag ccatagcgga cggtatagct tgatttgagt
     2701 gctgcttggg tgccgaagga gcggtacgcg ctcgttactt tcaaatcaat gtcgtcttca
     2761 gcagcatcgg tgagcaggtc ctcaaggaag ggatacgctt ctttctcaaa atactcatcc
     2821 ttatcatcag cgacccatgc ttcaggaatc tgggtgagtg cagcgggcac atagttatcg
     2881 gaaagaaaga atacttttga gtacttttgt aagagttcag ggtcaatctt cgatagctta
     2941 tcgagcttgc cgacggtgcc cgagatgtcc tgaatctttt cgtcgaatgc gtcattcctt
     3001 tctttctctg tctcatatgc gtcttccagg actgcatact cagtgcgaac agcgtcaagc
     3061 gcaacgtcgc gctctgcgat agtggtcgtt gcggtagcaa gctctgtgcg tacctgatcc
     3121 agggagcgga tagaggaata ccagccgtag cctattcccg ccaaggcaat tactcctatg
     3181 actactaggg cgagtagagg gtgtttcatc gccagaatgc taccacggga agggagtatg
     3241 gcgtattata tccctatgag tgcgtcgccc gaaagagctt ttaaccatca agcgcatacc
     3301 gcagacggag agaccctaaa gggggtggaa gcgcttacgc gggccgagct ttccgcaatg
     3361 aatagaaagc tcatgaccat cgcactttcg ctcaccgata aacatgtagc gaaagcaaaa
     3421 gatcttgttc aagaaacatt tgaacgcgcc ctcaaaagta aacacacctt tcaagagggg
     3481 ggtaaccttg aagcttggct cgctacaata ttgcggaatc agtattattc cagcaatcgt
     3541 cgcgccaagt tttgggatac tgaggatcct gaaacgagaa tgcgcgagat gtccgtatct
     3601 gctgagcaag atggagaaat agattccaaa caaattgacg cacttcttga gaagttgcca
     3661 gaacgattcc gcattgcact acaaacggtg cgcgatggag gtgattacga agaggtgggg
     3721 catcgtgtcg ggttgaaacc agaaagctta aagtcactca tgaatcgcgc acgcaaagcg
     3781 tttgctaagg tgttatatga cgccggttac atcgattcag atgatgagct tgtggagcga
     3841 tttctcgata aggaggtaaa aaagagaaag aaataatctt ccctacgttt ttccgttatg
     3901 tcgttgccac tcaacgatat gtccggcgtc ccgctcaatc tttgaggcat ggcgtaggta
     3961 cttccgattc tcttcaggca cgagagggag gtgaggtgcg tcgcgtagcg cgtgggcgat
     4021 atgccagagc ggctcctcac tctctatcca ggtagtgccg aatcgagaga ggctcatcgg
     4081 ctttgaatag gcgcggagcg tcttaaggcc agaggcgttc atgaagtact cgtggaaata
     4141 ggaagcggca agctcgcgca ctgatgaata gatgggatca cggaaccgta gtacggcatg
     4201 gttagttttt gagagcgctc cccagtatcc gttacgccga tacagcacaa cggcatgatc
     4261 atcgtccata ggaagcgtac gcaggttcat aaggagtggt ttctcacccg cttcatgaag
     4321 tgcggcgtac gcaagaagcg ctccttcaag gcagtgagcc tttttctctc gtatgaccat
     4381 aagtgggcta tatgaggtct cgcctttctt ttcataattg aaagggagac tatcaaggaa
     4441 gtcctgtatc tttgctggcg tagagagttt cttgaagagt gcgcgctcgc tcgcggtaaa
     4501 tcccgtcatg aagacaggat agcatcgagt gacatcagtc atgaatatag cgaaggctcg
     4561 tcatctttcg tatactgaac acaatcatga accgatcttt cactcttttc gcgcttaccg
     4621 cgctcatagg tgctaccgca ctcattccta ccagcgctcg tgctgccgag cttgaagtct
     4681 ctggatggat tccgtactgg gccgtctctg caggcgtacg cgaagcaggg aagcatctcg
     4741 atgaactctc cgtcatccac ccctttagct attcggtgaa cacggacggc aaaccaaaag
     4801 atcttgccaa tatgggcaaa agctcctggc agcgcctcat tcgtaacgcg aaggcagatg
     4861 gcgtcgcggt cattcctacc atcatgtgga gcgatacgaa ctccattcac cgcattctct
     4921 ccgacgagaa gcttcgtgcc gctcacgtga aagcgatagc cgatctcgtg aagaaagaaa
     4981 agtatgacgg tatcgatatc gactatgagg gcaagcttgc gaagacgcgt ccgtactatt
     5041 cgctcttctt gaaagagctt gatagcgcac ttggggcgaa gaccctttct tgcacgatag
     5101 aggcacgcac accgcccgag tccctctaca cgactattcc cgcaaccttg gagtacgcga
     5161 acgatttcgg gcagataaac aaatactgtg accgcgtcac gctcatgacg tatgaccagc
     5221 agcgcgctga cttgaaatta aatagcgctc gttcgggtgc cccctactac ccgatagcgg
     5281 atctcgagtg ggtgaagaaa gtggtggcgc ttgcgagcaa gtccattgat aagaataagc
     5341 ttgtcatcgg tgtcgccacc tatggccgcg aagtggaggt caccgtctcg ccaaactggt
     5401 tccaagcata ccgacagatc tcttcggtct ctgacgaata tgcgcgcgac accgcggaca
     5461 agcatgacgc ggacctgttc cgtaacggcg cgggtgaact ttcatattca tacgctcctg
     5521 acaaagcgac gcagaagctc cttgcctcag tctcacttcc gaaaagcacc cgtccgcgcg
     5581 acgaagctgc tgcccgcgcg cttgcgtacg ctgataagac ggggaacacc gtaaagattc
     5641 gcaccgtgtg gtggagtgac gcgaaagcga tagaagagaa ggcagacctt gcccgcgcgc
     5701 taggcgtaaa gggcatcgcc atattcaaga tagatggcgg ggctgacgaa gatctctggg
     5761 attcgttcta gtttgataag aaagatttta gtgctatgat gcgaacgccc cgcacaaccg
     5821 cggggtttat cgtgtttata aggggaatgt aggttgaaga ccaacttaat cgtagtgctt
     5881 ccgttgatgg ctttcgggtc atgggcagga gcctcctctg cacacgcgga cgaagttgca
     5941 gtaacgtctc ctgcagactc ggaccaagtg gcggcgctgt caaacgtctc attcgtcgat
     6001 accgaagtca gtcttcgaac cgactacatg gacaatgttg tcagctacgc gccaggttcg
     6061 ggcacgccgt ctcaaaatcc gagcggcatg gtctccggga tcaacgtgcg ctatctcttc
     6121 ccggtcggtc ggttccggct gggtgcggag gcgtacgtcc ttggcggcca cctcgaagac
     6181 gaggtggacg acggtttcta tatcgatgaa ggcaccgtta tcgaccttac gtatgggctg
     6241 gacgttattg cgggcgtcgt gctcggcagc aacgtcttgg tctatgcgaa agggggtgtt
     6301 tcctacgctt cggcgaaggt gacgaaaaac tgtcctccgc cggtgaacgc gcacttcggc
     6361 tactgcagca cacgcggtcc gttcgatcac gaaacgaacg ttctgtggca gggccaaaac
     6421 tacggcgccg gcatctcggt gcgtctcggc cgattcaacc tgaccggtga ctaccaacac
     6481 aacgacttcg acgccctcag tggcgtctcc ggtcagaacg cgttgggtca agacgtgacc
     6541 tacaacaaca tggctcttgc gggcgaccag tatggcgtcc gcatcggcta caacttctaa
     6601 cttccggcat tcgtgccgtg aagctaggga ggctcgacag caatgtcggg cctcttttct
     6661 tttatgtgct attcacgtat ctttcaatga ttcatgcaaa agcgaaagaa aaaattcgag
     6721 ccgggaaatt tcaaccttct ggtcaagcgc tcaacgtctg gccttgggct atttgccggt
     6781 gaacccatta agaaaggttc ctgcatcatc gaatacaaag gacgaacgat taccgaagca
     6841 gaagcctata ccagccgcag caagtatctc tttgaaatca actcaaagaa aacaattgac
     6901 ggaagaccaa aatggaacaa ggctggctat ataaatcact cctgcgttcc gaacgctgaa
     6961 cctgaaatcc gtacggcacg agtgttcatc atggcgaaaa agaacatcaa ggaaggggaa
     7021 gaaattacct acgactacgg cgacgaatac ttcaatgact atctagcgcc aggaggctgc
     7081 cgctgcccga agcatcgggg aaagaagacc gctaaaagat aaggaaccag aatacgaggg
     7141 cgaggaggat gcgatatatc gcgaacggta cgaaggtaaa cttacgcacg aacccaagcg
     7201 ctgcttttat aacaatgagg gcgaccacaa aggcagcgag gccgcccgta agaagcaagc
     7261 cgacattacc tccatcgaac acgccaggat tcttcataat gtcgtaggcg gtagcagcaa
     7321 gaagggtagg gaccgcaaga aggaaggaga agaccagaat agcggtacga gggataccga
     7381 gcataaggcc gcccatcacg gaggcgccgc ttcttgatac tcccgggata atggcaactg
     7441 cctgggcaag accaataagg aacgattgct tatacgtaat atcagttact gctccttcac
     7501 tctgttctcg ttt
//
LOCUS       DAORIN010000020         7202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1867_length_7202_cov_5.297887, whole genome shotgun sequence.
ACCESSION   DAORIN010000020 DAORIN010000000
VERSION     DAORIN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1867_length_7202_cov_5.297887"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..415
                     /locus_tag="PK109_01405"
     tRNA            345..415
                     /locus_tag="PK109_01405"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Pro,seq:tgg)
     gene            complement(533..745)
                     /locus_tag="PK109_01410"
                     /pseudo
     CDS             complement(533..745)
                     /locus_tag="PK109_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019960968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(814..2043)
                     /locus_tag="PK109_01415"
     CDS             complement(814..2043)
                     /locus_tag="PK109_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRH24234.1"
                     /translation="MIQHFLSVGAGILFAVWASVSAHYQEFRGPVAVPTATTSHEVVV
                     QSPGVGATPSPFDTVIGEIIKSASSTPRATTTKPAPAKPVGPVLPTTPSVPFKPLPLP
                     TPPPITPTSPSNPTPVTPTPTAPSVDTKLSDEALLKGAIVNIICLPGGGLRGSSGSGV
                     VIDSRGIILTVAHVAQNFLLRDYPTKGAGTCYIRTGSPATNAYAADLIFISSAWLGEN
                     KATFLQSKPVGTGENDFAFIAITRSITTKALPTRFTAIPLSPTGTDIEEDDEVGTGSY
                     AAEFLTSSQIRSSLYPTIKFAKVEDVYTFGRNTTDIFSVAAGSAAQEGSSGGGVMNSE
                     DRLIGLISTRTSKPDLALRSLQAITMDHIRRSFRADMGSDLDSYLKGDISTLITNFKP
                     TAAELLADLAEDIDDAR"
     gene            2108..2181
                     /locus_tag="PK109_01420"
     tRNA            2108..2181
                     /locus_tag="PK109_01420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2140..2142,aa:Gly,seq:tcc)
     gene            2263..3114
                     /locus_tag="PK109_01425"
     CDS             2263..3114
                     /locus_tag="PK109_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24235.1"
                     /translation="MGIESDPRRTNPDERMKNPHEDEPTDLVIETVSSPENLLDQETR
                     RVRWYLKEWKRYKELGYDNVIRLPSGIDPENESITDDEIRAAIGREFGDNQADYEAYA
                     RTFQETWNAMQEKMLPLMKQLYGFSPVGHFRIAPTAYGTGGGSLEKGEPVFFRLPKFR
                     PNAGGTPITEVEAITHEILCHECTAGLREKTAIDDSPIFATHQDVKEVLMDYLGRTLL
                     VRSGLMKREEVRMVGGGSVPEGFDFDALYYANPQNPDENNLRYEGRLPELVVAITEKI
                     KEMSQAA"
     gene            complement(3243..3626)
                     /locus_tag="PK109_01430"
     CDS             complement(3243..3626)
                     /locus_tag="PK109_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24236.1"
                     /translation="MFSSENSFTVLYTTDVSKTAAFFKNLGVAVKQEEADKVVVGFGS
                     FDLHYILNTSEPFEAYTYIAVSGSYGQGAIFYVETTDINELVERIPAAGGTLKSEVFQ
                     NKWNCRELLTEDPNGYKFAFYQAIS"
     gene            3793..4974
                     /locus_tag="PK109_01435"
     CDS             3793..4974
                     /locus_tag="PK109_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HRH24237.1"
                     /translation="MTNVFMRTIGVAALSIALAFSAAVMLAPSKAAAASCGFSRDLMV
                     GVSGEDVRCLQMYLNNSGYVIASTGVGSPGNETSLFGGFTKIAVAKWQAANGLPSSGM
                     FGPASRAKYATLAGGSTPTTPTTPTTPSVPTSGVAAARTAILDALDAYDTAVEDINDA
                     DDDGDDVDDAEDMLDDARDALFDAFTAFLNGNVTGATSLARNAESRAQDASDEAGGSS
                     NDDDDDDNDGDESDAEDAVDELDEAIDDAQDDIDEAEDDGDDVEEANDLLDEARDALD
                     DAEEALDDEDWEDAIDAADDGMDLVNNALDEIGIENGDEGDAEDAIDDAQDAIDDAWD
                     EINDAEDDDIDTDEAEDLVDQAEDELADAEDDFDDEDWDGAIEHAEEAMDLVEEALDS
                     L"
     gene            5095..6240
                     /locus_tag="PK109_01440"
     CDS             5095..6240
                     /locus_tag="PK109_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRH24238.1"
                     /translation="MEPLASRIRPKTLDEVQGQEHLVGEGKPLRVAIEKKHRFSFILW
                     GPPGTGKTTIARIYAAALDAELYELSAVSAGKEDIRRIVGDSREGKLALGKPKVLFLD
                     EIHRFNKSQQDFLLPYVESGELTLIGATTENPSFEVIPALLSRARVFVLESLSEESLK
                     AIVKRTKIKMGKGSFDWLLGYAGGDARKALTMLEGAQNLYGEVTVDTLNRAEESSHLR
                     YDKKGDEHYDTISAFIKSMRASQPDAAMYYLARMTEAGEDPLFIARRMVIFASEDIGV
                     AQPTALVVANAVFRACETIGYPECAISLAHGVAYLANAQKDRSAYDAFRAAQADVQAT
                     GNLPVPKHIRNAPTKLMKDLGYGKGYTKYDTESYLPEKLKGKKYFEK"
     gene            complement(6296..6928)
                     /locus_tag="PK109_01445"
     CDS             complement(6296..6928)
                     /locus_tag="PK109_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24239.1"
                     /translation="MRTLAIVAALFLTGIAAPAFADGIEKRTVQIRIATESKNVQQIC
                     EGLQAREIMYSTLEFCVADTIRRNGFSSVEDSRSRATLAVFVYDGEAARALAIVDPTN
                     VPRMVEVNDPPPRETGTSPWWPWVALLIAVVAGGLILAARNNHSSQNDEFSEASEAED
                     NTEVEAHNANTTHAEVDEPPVPEPEPVKPTTATPITPDDGPAPPEGEVKS"
     gene            complement(7070..>7202)
                     /locus_tag="PK109_01450"
     CDS             complement(7070..>7202)
                     /locus_tag="PK109_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRH24240.1"
                     /translation="GIFVAKARPARTGRNPRTGETIRIAATRTPKFRPAKALKDAVK"
BASE COUNT         1821 a         1739 c         2003 g         1639 t
ORIGIN      
        1 agaaaatagg agttcgattc tccttagccg caccaaggta tgaagcgaaa acggccctga
       61 aagccatttt tgcgttatgg ggaaaacagg ggataaaaca ggggatatgg ggataaagga
      121 caataaggac gtcctaatca tttctgagat aagcgtttta tactcaataa ataggctgtc
      181 gtatttaaag tcagtacctt cgcggacatc tgttccaaag acattctcgc attggggaca
      241 aatcccgaaa ccgtagatgg acttaaagag caatttagat aaagagcact gatcccggtt
      301 aaaaacctgc cggaatttaa ctatcgtgct atggtatcga gcaacggggt gtcgtatacc
      361 ggtagtacga ctgctttggg agcagttaga cggggttcaa ttccccgcac cccgacacgt
      421 taggtttcat agctgaacaa gaaaaccgcc gattacgcac ttgcgtaacc agcggtcttt
      481 cttttacttg ctcggtggct cgttagcctt gttgttgcgt ctgtgccacc accaggcctt
      541 caaagaaata ttggattggc acaccgagtg ccacagcggt ttcgtatagc cttgatgcag
      601 ggatacggtt cgatcctgct tcgtatcgct gaatttgttg aaaacgggtg ccaaccgctt
      661 ccgcgagctg ttgttgtgtg agtccgagaa ggcgtcgccg ccgccggatc cgtttcccga
      721 taaatcggtc aacatcgttt gccatgctag taacctctcg tatctatgca atggactcgt
      781 tgaccatacg atgaatattt gcaccggtca atgttaccgt gcgtcgtcta tgtcttctgc
      841 aaggtcagca agcaactcag ctgcagtcgg tttgaaatta gtaatgagcg tggagatatc
      901 gcctttgaga taggaatcga ggtcagaacc catatcagcg cggaagctgc ggcgaatatg
      961 atccatggta atggcttgga gagagcgaag tgcaaggtca ggctttgaag tacgggtaga
     1021 gataagaccg atgaggcggt cttcgctatt cataacgccg ccccctgaag acccctcttg
     1081 agccgcagat ccggcagcaa cagagaagat atcggtagtg ttacggccaa aggtgtacac
     1141 gtcctcaacc ttcgcgaact ttatggtggg gtagagagaa gagcgtatct gagagctcgt
     1201 aaggaattca gcggcatagg agcccgtgcc tacctcgtca tcctcttcaa tatcggtgcc
     1261 ggtaggggag agagggattg ccgtaaagcg ggtcggaagt gcctttgtgg taatgcttct
     1321 cgtaattgca atgaaggcaa agtcgttctc acccgtacca acgggctttg actggaggaa
     1381 tgtcgctttg ttttctccga gccacgcaga cgatatgaag atgagatctg cagcgtaagc
     1441 gtttgtagca gggcttccgg tgcgtatgta gcaggtgcca gcgcctttag tggggtagtc
     1501 gcgtaggaga aagttctgcg caacgtgggc gacggtgagg atgatgccgc gggagtctat
     1561 gacgacacca gagcccgaac ttccgcgaag gcctccgccc ggaaggcaga tgatgttcac
     1621 gatagctccc ttgagaagcg cttcatcaga aagcttcgta tcaaccgaag gggcggtagg
     1681 agtgggggta accggcgtcg ggtttgaagg cgacgttggg gttatgggcg gaggtgttgg
     1741 aagcggaagt ggcttaaacg gtaccgaagg agtcgtggga agtacgggcc ctaccggttt
     1801 tgcaggcgcc ggctttgttg tggtcgctcg cggcgttgag ctcgcgctct ttataatttc
     1861 gccgatgacc gtatcaaacg gggaaggagt agcgccaacg ccggggctct gtactactac
     1921 ttcatgggag gtagtagcgg tagggacggc taccggccct cgaaattcct ggtagtgggc
     1981 gcttacggag gcccatactg caaagaggat accagctcct acagaaagga agtgctgaat
     2041 catatccgta tgctacgcca gaacggacat gaaatacagc gcattacgtg gtattctcct
     2101 gcgtgaagcg ggattagttt aatggtagaa ctacagtttt ccaaactgtt agcaggagtt
     2161 cgattctcct atcccgcacc aatctgtggt agttcaagag accaagatgc tcagtcggca
     2221 actgatttta ggagtttcga aaatgaagtt atacttcctc acatgggaat cgaaagcgat
     2281 ccgcgccgaa ccaaccctga cgaacgcatg aaaaatccgc acgaagacga gccaaccgac
     2341 ctcgttattg aaactgtctc aagccccgag aatttactcg accaagaaac caggcgtgtg
     2401 cgctggtatc tgaaagagtg gaagcggtac aaggaacttg ggtacgataa cgtcatccgt
     2461 cttccttctg ggattgaccc tgaaaacgaa agcatcaccg atgacgaaat acgcgcggcc
     2521 atagggaggg aatttggcga caaccaagcc gactatgaag catatgcacg caccttccag
     2581 gagacatgga atgcgatgca agagaagatg cttccgctta tgaagcaact atatggattt
     2641 tctcctgtcg gacatttccg tattgcgccc actgcgtacg gtaccggtgg aggctcactg
     2701 gaaaagggcg agccggtatt tttccgactc ccgaaatttc gtccaaacgc gggcggtaca
     2761 cctataacag aagtcgaagc catcactcac gagatccttt gtcatgaatg cactgcgggt
     2821 cttcgcgaga aaaccgcaat agatgactca cctatatttg caacacatca agacgtgaag
     2881 gaagtgctca tggactatct tggacgaaca ctccttgttc gttccggcct catgaagcga
     2941 gaagaggtac gtatggttgg cggaggcagt gtaccggaag gattcgattt tgatgcactc
     3001 tactacgcca accctcaaaa ccctgacgaa aacaatttac gttatgaggg gcgactccct
     3061 gagttagtag tggccattac ggaaaaaatc aaagaaatgt cacaggcggc ataagaccgt
     3121 gcttgatctt ttagaccgaa gatacctcag gtcgattcta aggaaccctc aaagcgcccc
     3181 acgctaccta gtccgagact tgggggcacc ttatagcgct agtccaagag accgtagagt
     3241 ctctatgaaa tcgcttggta gaaagcgaac ttatacccat ttgggtcttc ggtcaggagc
     3301 tcacggcagt tccatttatt ctgaaatact tcagatttaa gcgtgcctcc ggcagcggga
     3361 atacgctcaa ctagttcatt aatgtcggtg gtctcgacat agaaaattgc cccctgtccg
     3421 tacgagccag acaccgctat gtacgtatag gcctcaaacg gctcgctagt gttcaaaatg
     3481 taatggagat caaaggaccc gaaaccgacc accaccttat cggcttcctc ttgttttact
     3541 gctaccccga gattcttaaa gaacgcagcg gtcttggaga catcggtggt atagagaacc
     3601 gtaaatgagt tttctgagct aaacatatcg taaggctatc atacggggaa ttttccttga
     3661 aaagcttgat tcacggagtg ctctttagat ttccttccta gttcacatca aatcctcata
     3721 caacaacatg ttatctttag tacatagcat cgtacgaaac gtgcgagcag gtataaataa
     3781 ctactagtac ttatgactaa tgtattcatg cgaacgatag gtgttgcggc cctatccatt
     3841 gcgctcgcct tttcggcagc ggtaatgctg gcgccttcca aggcagccgc cgcaagctgt
     3901 gggttctcac gtgacctcat ggtcggcgtt tctggagaag atgtgcgttg tctccagatg
     3961 tatctcaata actcgggata cgtcattgca agcacgggag tagggtctcc tggaaacgaa
     4021 accagcctct ttggtggttt cactaagata gcggtagcaa agtggcaggc tgcgaacgga
     4081 cttccaagct ctggtatgtt cggtccagcg tcacgcgcta agtacgcaac gcttgcggga
     4141 ggttctacac caacgacgcc gaccactcca accacgccaa gcgtaccgac ttcaggggta
     4201 gctgctgcac gcacggccat tcttgacgcg cttgatgcct atgacacggc agttgaagac
     4261 attaacgatg ctgatgacga tggtgatgat gtcgacgatg cagaagatat gctcgacgac
     4321 gcacgggacg cattgtttga cgccttcacg gccttcctca acggaaatgt taccggtgcc
     4381 acgagccttg cacgtaacgc agagtcacgc gcccaggacg cttctgacga agcagggggt
     4441 agcagcaacg atgacgacga tgatgataac gatggcgacg aaagcgatgc tgaagacgcg
     4501 gtcgacgaac tcgacgaagc tattgatgac gcccaggacg acattgatga ggctgaagac
     4561 gatggtgacg acgtagagga agctaacgac cttctcgacg aagcacgtga tgcgctcgat
     4621 gacgctgaag aggcgctcga tgatgaagac tgggaggatg ccatcgacgc ggctgatgac
     4681 ggtatggatc tcgtaaacaa cgctcttgat gaaataggaa ttgagaacgg tgacgaggga
     4741 gatgctgaag acgccattga tgacgctcag gacgccattg atgacgcgtg ggatgagatt
     4801 aacgacgctg aggacgatga catcgacacg gacgaagcag aagatctcgt tgatcaggca
     4861 gaagatgagc ttgctgacgc tgaagatgac ttcgatgacg aagattggga cggcgctatc
     4921 gagcacgctg aagaagcgat ggacttggta gaggaagcac tcgactctct ctaagagagg
     4981 gaagtaaaga gagaaaaacc gcgccctaga gcgcggtttt tctttataca aagctgtgta
     5041 ggtcttgggt actatgctaa tcgcacttta atctgaggag cgtaggatac agggatggaa
     5101 ccgttagcgt ctcgtatacg cccgaaaacc ctcgacgagg tgcagggaca ggaacacctg
     5161 gtcggagaag ggaagccgct ccgcgttgcg atagagaaga agcaccgctt ctcgttcatt
     5221 ctgtggggac cacccggcac cgggaagacc accattgccc gcatctatgc ggcggcactt
     5281 gatgctgaac tctatgaact ctctgctgta tcagcaggga aagaggatat acgaaggata
     5341 gtgggggact caagggaagg gaagttggca ctcggaaaac caaaggtcct gtttcttgat
     5401 gagatacatc gattcaataa gagtcagcaa gattttcttt tgccgtatgt cgagagtggg
     5461 gaactaacgc tcataggcgc cacaacggag aatccgtctt ttgaagtgat tcccgcgctt
     5521 ctctcgcgtg cccgggtgtt cgtgctcgag tcactctctg aagagtcact caaagcaatt
     5581 gtgaagcgta cgaaaataaa gatggggaag ggaagtttcg actggttact tgggtatgcg
     5641 ggaggcgacg cgcgcaaagc cctcactatg cttgagggcg cacagaacct gtatggagaa
     5701 gtgaccgtag acacacttaa tagagcagaa gagtcctcac acctccgcta tgacaagaag
     5761 ggagacgaac actatgacac cataagcgca ttcattaaga gtatgcgcgc gagtcagccg
     5821 gacgccgcta tgtattatct tgcccgcatg actgaagcgg gagaagatcc tctttttatt
     5881 gcacgccgca tggtgatatt tgcgtcagaa gatattggag tagcacagcc cacggcgctc
     5941 gtcgtcgcaa acgccgtatt tcgtgcgtgt gagaccattg gctacccaga gtgtgcaata
     6001 tcacttgccc acggcgtggc gtaccttgct aacgcccaga aggatcgctc agcgtatgat
     6061 gcgttccgtg cggcacaagc ggacgtacag gccacgggta accttcctgt tcctaaacat
     6121 atccggaacg cccctacgaa acttatgaaa gacctaggat atggaaaagg gtatacgaaa
     6181 tacgatacgg agagctacct gcctgaaaaa ttaaagggta agaagtattt tgagaaataa
     6241 aaagtaacgc gccgttgccg gcgcgttttt ctaaagttca aattgtcgcc ctattctagg
     6301 acttcacttc gccttctggc ggcgccggac catcatccgg agtgatggga gtcgccgttg
     6361 ttggtttgac tggctcaggt tctggcaccg gcggctcgtc gacttccgcg tgagtagtat
     6421 tggcgttgtg cgcctcaacc tcagtgttgt cttcggcttc ggatgcttct gagaactcgt
     6481 cgttttgcga agaatggttg ttccgcgcgg ccaggatgag accaccggca acgacggcaa
     6541 tcagtaatgc tacccacggc caccacggac tggttcccgt ttctcttggc ggaggatcat
     6601 tcacctctac catcctcggc acgtttgtcg ggtcgacgat agccaaggct cgcgccgctt
     6661 cgccgtcgta gacgaaaacg gcgagggtcg cacgtgagcg gctgtcctca acagacgaga
     6721 agccattccg tcgtatagtg tcggcaacgc agaattcaag cgtgctgtac atgatttctc
     6781 gggcctggag cccttcgcaa atctgctgta cgtttttgct ttcagtagcg atccggattt
     6841 gaaccgttcg cttttcaatg ccatccgcaa atgcgggtgc cgcgatcccc gtgaggaaga
     6901 gcgcggctac gattgctaag gttctcatgg aaccccttct ttgtttttag cttcagaaag
     6961 gactggacgc caaaacgccc aacccatatg gaccttacga ctattcagaa taaagtcaaa
     7021 tccccggggt ataccccggg gatttggcct attcgaacga aatcgtggct tacttaaccg
     7081 cgtccttaag ggcctttgca gggcggaact taggggtgcg ggttgcagcg atgcgaatag
     7141 tctcgccggt gcgtgggttg cggcccgtgc gcgctggacg tgccttcgca acgaagatgc
     7201 cg
//
LOCUS       DAORIN010000021         7069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1931_length_7069_cov_4.963359, whole genome shotgun sequence.
ACCESSION   DAORIN010000021 DAORIN010000000
VERSION     DAORIN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1931_length_7069_cov_4.963359"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2298)
                     /gene="ileS"
                     /locus_tag="PK109_01455"
     CDS             complement(<1..2298)
                     /gene="ileS"
                     /locus_tag="PK109_01455"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRH24241.1"
                     /translation="MADSAPKTDTNTREEQVLAWWKANRIFEKSVEKPAPKGDFVFYD
                     GPPFATGLPHYGHIVASVIKDAIPRFATMRGYRVPRIWGWDCHGLPIENIVEKELGFK
                     HKKEIKEYGIKKFNERCRETVLMYADEWAKIIPRIGRFVDMEHSYRTMDKSFMESVWW
                     VFKQIYDKGLVYEDYRSMHICTRCETTLSQTEVSEGYKDVKDISVTVKFPLKDEPETA
                     LLAWTTTPWTLPGNVAIAVGADVDYVKVQDEAGTYILGKEALTRRSAEFKNPNVIGEI
                     KGSALVGKSYTPPFDYYVNDTTLKNHENGWKVYAADFVTAESGTGIAHEAPAFGADDW
                     ELSKQVNLPFVQHVAFDGTMKPEVTAFSGMEVKPRSDDDKIRLATDIAVLKYLQENGS
                     FFSKENITHSYPHCWRCDTPLLNYATSSWFVSVTKVKDNLLENAKKVSWSPEHIKEGR
                     FGKWLEGARDWSISRQRFWASVIPIWVCETCKEKRVFGSASELEEASGTKVDDLHKHI
                     VDEVTVPCSCGGVMRRIPDVLDTWFDSGSMPYGSKHYPFEGKEEFDAVFPAQFIAEGQ
                     DQTRAWFYYLHVLAGALFQKPAFQNVIVNGIVLAEDGKKMSKKLNNYPDPMMVVEKYG
                     ADALRLYLLSSPVVQAENLAFSEEGVDEAAKKSIGRLANVLSFYKLYADDTAPSTDSA
                     HVIDRWILTRLAALVTEVTEGYEKYELHSATRPLALFIDDLSTWYVRRSRDRFKEEGS
                     DKTAALGTLRYVLRTLAHLMAPVTPF"
     gene            complement(2655..4334)
                     /gene="argS"
                     /locus_tag="PK109_01460"
     CDS             complement(2655..4334)
                     /gene="argS"
                     /locus_tag="PK109_01460"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRH24242.1"
                     /translation="MEERIRKAVETALATLGAEDVTFAVERPADPSHGDFAVNAAMAA
                     AKGLKKNPKALAEELVPLIEGALGDYVQSVTVAGPGFINLTLARSVITDALKDAQHSS
                     WGQGTVNSGRRVMIEYSNPNPFKEMHIGHLMSNVIGEALSRTIEASGAHVIRDTFGGD
                     VGPHVAKALWALKKKGVTDIGSAKEIGDAYAHGSNAYEESETAKAEIDELNTKIYDVV
                     GKQEEANNLSEEDRELLALWRKGREVSMEEFSRIFKVLGTKFDYVLYDSDTTKGGISA
                     VMDGVEKGIFEKSDGAIVYKGEKKGLHTLVFITSRGTPTYETKDVGLAFLKEERCATD
                     SVIIITAVEQVGHFKVFLAALEDIAPLLAKKTQHVPHGLLRLPTGKMSSRKGNVITTA
                     ELIQSVIELAQEKNEDPIVAEQVAIGAVKYMILRQAPGGDIIFDPEKSLSLDGDSGPY
                     LQYSLVRAKSVLSQADLQPSGEDAPETPYLLERLITRFPEVVARAEAEHAPHHITQYL
                     TQLAGEWNSFYAAERIRGGEHEAYKLLVAEAFVTTMTNGLTLLGIPTPEKM"
     gene            complement(4588..4737)
                     /locus_tag="PK109_01465"
                     /pseudo
     CDS             complement(4588..4737)
                     /locus_tag="PK109_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008228584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
     gene            complement(4802..6097)
                     /gene="glyA"
                     /locus_tag="PK109_01470"
     CDS             complement(4802..6097)
                     /gene="glyA"
                     /locus_tag="PK109_01470"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRH24243.1"
                     /translation="MYDYIEAQDKEVYDAIEGEEKREREGLELIPSENYVSMAVREAM
                     GSVFTNKYSEGFPGKRYYGGQTYTDIVENIAIERAKKIFGADHANVQPLGGAAANIAA
                     YFSWLEPGDTVLGMDLAHGGHLTHGSPVTYIARLFKFVRYGMKNIETGEIDYEALRQT
                     ALAEKPKLILGGFSGYPRELDWQKISDIAKEVGAVAMADVAHIAGLIAGGAAKNPFDY
                     GFQIMTTTTHKSLRGPRGAMILSKGTVSNPMKAPEKTIENLPTLIDRAVFPGFQGGPH
                     MHQIAAKAVSFGEALKPSFKTYAHQVVANAKAMAEVFNENKVRLITGGTDNHLLLADV
                     WTSLEISGGDAEKLLDEAGITLNKNSVADDTRKPFDPSGIRFGTPAITTRGFKEKDCA
                     RVAELMIDILKQRDEKTVASSKREITQMAEKHPVPESFE"
     gene            complement(6154..6837)
                     /gene="tmk"
                     /locus_tag="PK109_01475"
     CDS             complement(6154..6837)
                     /gene="tmk"
                     /locus_tag="PK109_01475"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRH24244.1"
                     /translation="MQKGIFVVIDGIDGSGKATQSRLLTERFRAEGRAVEKVDFPRYG
                     TPLFGELLGECLAGKHGDFLHLDPKIASTLYALDRYEASEQIRAWLAEGKVVIADRFA
                     SSNQIHQGGKIMDAAKRDAFLAWIDKMEHEVLNVPRPDAVVYLRVPIETSLELLSKER
                     EAKNTALDGETADVVEKDRMYLERSFESAERLSTADNWHAIDCVENGAIRSPEAIHED
                     VHKAVSALL"
     gene            6852..>7069
                     /locus_tag="PK109_01480"
     CDS             6852..>7069
                     /locus_tag="PK109_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020806895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRH24245.1"
                     /translation="MGHMEKAYLDLLKKVMDEGVDRDDRTGTGTRALFGQTMRFNMAD
                     GFPALTTKKLGWKAVKSELLWFLEGTGD"
BASE COUNT         1556 a         1950 c         1767 g         1796 t
ORIGIN      
        1 gaaaggcgtt accggcgcca taaggtgcgc aagagtacga agcacgtagc gaagcgtgcc
       61 gagagccgca gtcttatcac ttccctcttc tttaaaacga tcacgtgagc ggcgcacgta
      121 ccacgtcgag agatcgtcga tgaagagcgc caagggacgc gtcgcgctat gaagctcgta
      181 cttctcatac ccctcagtta cctcggtcac aagtgctgca agacgcgtga gtatccaacg
      241 atctatgacg tgcgcactgt ccgtcgatgg cgctgtatcg tcagcataga gtttgtagaa
      301 cgagagcacg tttgcgaggc gtccgatgct tttcttcgct gcttcatcaa cgccttcttc
      361 ggagaacgcg agattctctg cctgcaccac cggagacgaa agaaggtaga gacgaagtgc
      421 gtcagcgcca tacttctcca cgaccatcat tgggtcagga tagttattaa gcttcttcga
      481 catcttcttc ccatcttcgg caagtacaat cccgtttacg atgacgttct ggaacgctgg
      541 cttctggaat agcgccccgg caagcacatg gaggtaatag aaccaggcac gagtctggtc
      601 ctgaccttca gcgataaact gcgctgggaa gaccgcatca aactcttctt ttccttcaaa
      661 tggatagtgc ttcgagccgt acggcatgga gcctgagtca aaccaggtat caagcacgtc
      721 cgggatacgg cgcattactc ccccacacga acacggcacc gttacttcgt cgacgatgtg
      781 cttatgcaaa tcgtctacct tcgttccgct ggcttcttct agttcggacg cactgccgaa
      841 cacgcgcttc tctttgcagg tttcgcatac ccatattggt atgacgctcg cccagaaccg
      901 ttggcgcgaa atagaccaat cgcgtgcgcc ttcgagccat ttgccgaagc gcccttcttt
      961 gatgtgctcg ggagaccacg atactttctt cgcgttctca agaagattgt ctttcacctt
     1021 cgttacggaa acgaaccatg atgacgtcgc atagttcaag agcggcgtat cgcagcgcca
     1081 gcaatgcgga taggagtgcg taatgttttc tttagagaag aaggatccgt tctcctgaag
     1141 atacttgagg acggcaatat cagtagcaag tcgtattttg tcatcgtctg agcgtggctt
     1201 cacttccatg ccggaaaacg ccgttacttc aggcttcatg gtgccgtcga acgcgacatg
     1261 ctgcacgaat ggaagattca cctgcttgga gagttcccag tcgtctgccc cgaacgccgg
     1321 cgcctcgtgc gcaataccgg taccactttc agcagttacg aaatccgcag catacacttt
     1381 ccatccgttc tcgtgattct taagggtcgt atcatttacg taatagtcga aggggggtgt
     1441 ataggactta ccaacgagtg cacttccctt gatctctcca ataacgtttg ggtttttaaa
     1501 ctcagcactg cgacgcgtca gcgcttcttt cccgagaatg taggtgccgg cctcgtcctg
     1561 tacttttacg tagtcaacgt cagcgcccac tgctatcgcc acattccccg gcagtgtcca
     1621 tggcgttgtg gtccacgcaa gaagcgcggt ctccggctcg tccttcaaag gaaacttcac
     1681 cgtgaccgaa atatctttca catccttata cccttccgag acctcagtct gcgacagcgt
     1741 ggtctcacag cgtgtgcaga tgtgcattga acgatagtcc tcgtagacga gtcccttatc
     1801 gtatatctgc ttgaacaccc accacacact ctccatgaat gatttatcca tggtgcggta
     1861 ggaatgctcc atatcaacga agcggcctat gcgcggaata atcttcgccc actcgtccgc
     1921 gtacataagc accgtctccc ggcagcgctc gttaaacttc ttgatgccgt actccttaat
     1981 ctccttcttg tgcttaaacc cgagctcttt ctctacgatg ttctctatcg ggagtccgtg
     2041 acagtcccag ccccagatac gcggaacacg gtagccacgc atggtcgcaa agcgcgggat
     2101 agcgtccttg atgactgagg ctacgatgtg gccatagtgc gggagtccgg tagcaaaggg
     2161 cggaccatca taaaagacga agtcaccctt cggagcaggc ttttctaccg atttctcaaa
     2221 gatgcggttt gccttccacc aggcaaggac ctgttcctct cgagtattgg tatccgtctt
     2281 aggggcgcta tcagccatat tcccctcatc ctatcgtctc aaaggggaaa atgaaaaggc
     2341 cgacgctcgc gcgccggcca gtccgtgagg ggtctacccc tcatcaggat ttatttttga
     2401 gttcttgggg aggacatctt ttgcgtgcca ccctcgaatc actccctctc tcggtttttc
     2461 cctttgagta ctcgtctgcg aatttcgttc gcgcacgatt gctcggaatc cgggttggag
     2521 ttgcactttt gggggtctcg agagccgtga gtacgacttc gtattgcgca ttcttaacat
     2581 agtcaccccg tggtttctca tcaacatcaa cacaatgtcg attcggcact gtatcacaaa
     2641 acatacagat cacactacat cttctctgga gttggaatac caagaagcgt aagaccgttg
     2701 gtcatggttg taacaaatgc ttctgctacc aaaagcttat atgcctcatg ttccccacca
     2761 cgtatccgct ctgcggcata gaacgagttc cactcaccgg caagctgcgt gaggtactgg
     2821 gtaatgtggt gcggtgcgtg ctcagcttcg gcgcgcgcaa ccacctcagg gaaccgcgtg
     2881 atcaagcgtt ccaaaagata tggcgtttca ggagcatctt ctcctgacgg ctgtaggtct
     2941 gcttgagaga gcacggactt cgcgcgcact aacgagtatt gaagatacgg acctgaatca
     3001 ccatcaaggg agagtgattt ctctggatca aagatgatgt cgccaccggg tgcctggcga
     3061 agaatcatgt acttcactgc gccaatggct acctgttctg cgacgatagg atcttcgttc
     3121 ttttcttggg caagctcgat aacgctctga atgagttcgg ctgtcgtaat gacattccct
     3181 ttgcgcgatg acatcttccc ggtaggaagg cgaagaaggc cgtgcggcac gtgctgcgtc
     3241 ttcttcgcaa gcaagggcgc aatatcttca agggccgcaa ggaacacctt gaagtgccct
     3301 acctgctcca ctgccgtgat aataatgaca gagtcagtag cgcagcgttc ttctttcaag
     3361 aatgcgagtc cgacatcttt cgtttcatag gtgggagtgc cacggctcgt gatgaagaca
     3421 agggtgtgaa gccccttctt ctctcctttg tacacgatag cgccatcgct cttttcaaag
     3481 attcccttct caacgccgtc catcaccgca ctgatgccac ctttagtggt atcgctgtca
     3541 tagagaacat agtcgaactt cgttccgagt actttaaaga tgcggctgaa ctcctccata
     3601 gatacttccc ttcctttgcg ccagagcgcg agaagttcgc gatcttcttc tgaaagattg
     3661 tttgcttcct cttgttttcc aacgacgtcg tatatttttg tattcaactc atcaatttct
     3721 gcctttgcgg tttccgactc ttcatatgcg tttgagccgt gtgcatacgc gtctccgatt
     3781 tcttttgctg atccaatatc agtaacgcct ttctttttga gtgcccagag tgcctttgct
     3841 acgtggggac ccacgtcgcc gccgaaggtg tcgcgaataa cgtgagcgcc ggacgcttcg
     3901 atggtgcgtg acaacgcttc cccgatgacg ttactcatca aatgcccaat gtgcatttct
     3961 ttgaatgggt ttgggtttga gtactcaatc atcacgcggc gcccactgtt taccgtcccc
     4021 tgcccccatg acgaatgctg cgcatctttg agagcatctg tgatgacgga acgtgcgagt
     4081 gtgaggttga tgaatccagg gccggcaacg gtaaccgatt gcacataatc accaagcgct
     4141 ccctcgataa gaggtaccag ttcttcagcg agcgcctttg ggttcttttt aagaccttta
     4201 gcggcagcca ttgcagcgtt tacggcaaag tccccgtggc tcgggtcagc cggacgctcc
     4261 acggcaaagg tgacgtcttc ggcgccaagg gtagcgagcg cggtttctac cgcttttcgt
     4321 attcgttcct ccatgaggga gagtgtagca aatcaaggct ccacaggctt gtgatgctaa
     4381 gaaaaacgcc cggcaatgcc aggcgttagg ttttcaagct ttgctcgatt atgaagtgct
     4441 ggtggcttct gctgccgttt cgacttgggc aagttcagcg cgagcggaag caagctcttc
     4501 gagtatgttt ttcgcttcgc cttctcttac ctcaagctgc cggatgcggc gcttcagatt
     4561 ttttgccttc gtctttgtgt cccccatttc aataagtatc tcgacacaaa ggtcgacgca
     4621 ctcgttgcaa atgaatgaga ccgggcccgc aatgagatgt ccgacctcaa attggttctt
     4681 gccgcagaac gaacaataga gcgtgggttt attctgggga gcttcagttt gaggcatggc
     4741 gccttctcct gataacaatc cggacatggg tgtccggacg gactatactc cagcccgata
     4801 cttattcaaa cgattcaggc accggatgct tttctgccat ctgcgtaatc tcacgcttgc
     4861 tggaagcgac agtcttctcg tcacgctgtt tcaagatatc gatcatgagt tctgctacgc
     4921 gtgcacaatc cttttcctta aagccacgcg tcgtaatggc aggggtgccg aagcgaattc
     4981 cagatgggtc aaatggcttg cgggtatcgt ccgccactga gttcttgtta agtgtgatac
     5041 cggcttcgtc aagaagtttc tcggcgtcac ctcctgaaat ttcaagcgac gtccacacat
     5101 cggcaagaag aagatggtta tcagtgcctc ctgtaatgag acgtacctta ttttcgttaa
     5161 atacttctgc catcgctttt gcgtttgcga ctacctggtg cgcatacgtc ttgaatgaag
     5221 gcttgagtgc ttcaccgaag gaaaccgcct tcgccgctat ctgatgcatg tgcgggccgc
     5281 cctgaaatcc tgggaatacg gcgcgatcaa tcaaggttgg aagattctct atcgtcttct
     5341 caggtgcctt catggggtta gataccgttc ccttcgagag aatcatcgcg ccacgcggtc
     5401 cgcgcaaact cttatgcgtc gtggtcgtca tgatctggaa tccgtaatca aaaggattct
     5461 ttgcagcgcc gcccgcgata aggcccgcga tgtgcgcgac gtctgccatt gctaccgctc
     5521 cgacttcttt ggcgatgtca gagatcttct gccagtcgag ctcgcgtggg tagccggaga
     5581 atccgccgag tatgagcttt ggcttttcag cgagcgccgt ttgacggagc gcctcatagt
     5641 ctatttctcc ggtctcaatg ttcttcatcc cgtagcgcac aaacttaaac aggcgtgcaa
     5701 tgtaggtgac tggtgagccg tgcgtgaggt ggccaccgtg cgcaagatcc atacctaaga
     5761 ccgtgtctcc tggttcaagc catgagaagt aggccgcaat gtttgccgca gcgccaccaa
     5821 gtggctgcac gtttgcatga tcagcaccaa agattttctt cgcgcgttca atcgcaatgt
     5881 tctcgacgat gtccgtgtag gtctggccac cgtagtaccg cttcccgggg aagccttcag
     5941 agtatttatt cgtgaacaca cttcccatcg cttcgcgcac cgccatcgaa acgtagttct
     6001 cagatgggat aagctcgagc ccttcacgct cgcgtttctc ctccccttct atcgcgtcat
     6061 atacctcctt atcctgtgcc tcaatgtagt cgtacatagc gtcctgttac gtaaataaag
     6121 cgcccggcga agaggcctca gatcatgagc ggattatagc aatgcggaaa ctgctttatg
     6181 cacatcctca tggatggctt caggagagcg aatggcgcca ttctcaacac agtcaatcgc
     6241 gtgccagtta tcagcggtgg agaggcgttc ggcagactca aaggaacgct ctagatacat
     6301 gcggtccttc tcaacaacat ctgccgtctc cccatcaagc gcggtattct ttgcttcccg
     6361 ctccttagaa agaagctcga gtgaggtttc aataggaacg cgaagatata ccaccgcatc
     6421 agggcgtggg acattcagca cctcatgctc catcttgtct atccacgcaa gaaacgcatc
     6481 gcgctttgca gcatccatta tctttccgcc ctgatgtatt tggttacttg aggcaaagcg
     6541 atctgcaatg actaccttcc cttcagcaag ccaggcacga atttgttcag acgcttcata
     6601 gcgatcaagc gcatacaagg ttgaggcaat cttcggatca aggtggagga agtctccatg
     6661 cttcccggca aggcattccc caagcagttc cccgaataat ggtgttccgt agcgcgggaa
     6721 gtctaccttc tccaccgcac gcccctcagc acgaaaccgc tccgtaagaa gacgcgactg
     6781 cgttgctttt cccgaaccat cgatgccgtc aatgacgacg aatatccctt tttgcattgt
     6841 gccctagtat actggggcac atggaaaagg cctatctcga cttacttaaa aaagtgatgg
     6901 atgaaggggt ggaccgggat gaccgcaccg ggacgggtac ccgtgccctc ttcggacaaa
     6961 cgatgcgatt caatatggcc gacgggttcc ctgccctcac cacgaagaag cttggctgga
     7021 aggcagtcaa aagcgaactc ctctggtttc ttgaaggaac gggagacga
//
LOCUS       DAORIN010000022         6978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1969_length_6978_cov_5.344070, whole genome shotgun sequence.
ACCESSION   DAORIN010000022 DAORIN010000000
VERSION     DAORIN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1969_length_6978_cov_5.344070"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PK109_01485"
     CDS             <1..1085
                     /locus_tag="PK109_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRH24246.1"
                     /translation="AIIFVDEIDAVGRVRGAGVGGGNDEREQTLNQILVEMDGFDPNE
                     KVIVMAATNRPDVLDPALLRPGRFDRRVTIDLPDRSDREAILKVHARKKPLGPDVTLN
                     VIAERTPGFSGAELHSLMNEGAILAARENRKEITQYDLIRSIEKVMLGPERKSHLLSK
                     KEKKVTAYHEAGHALVSSVLPHADPVHKISIISRGRAAGYTLKLPFDDKKMQSKKQFK
                     DDIAATLGGYVAEEMIFGDITTGPSNDLAVLTALARDMVARYGMSDVIGPVAFSAERN
                     YAGETTYSQDIAGKIDTEVSRIIAEARTKATDVLTNHKKALDAIAETLVEVETLERDA
                     FEKLLIANGITPKKREEDETPVTVTD"
     gene            1144..1818
                     /locus_tag="PK109_01490"
     CDS             1144..1818
                     /locus_tag="PK109_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRH24247.1"
                     /translation="MAKYELHDRAIHMRKLGMSYTQIKDELGVSKSTLSGWLQHYPLS
                     PERLKELRTNTERQIERIRDTKSRKRSARLAAVREQVERGISHLSDREILIAGLFLYW
                     GEGGKTKPYTTTISNTDPAMLLFFIHWLELLGVDRNRIRAYVHLYADMDAEKEVKYWS
                     TTLKLSKNAFRKPHIKNSNRCELTYKQRFSHGTCNIIFDNRDTAEYVSQALEVIRARF
                     AQGINP"
     gene            1831..1904
                     /locus_tag="PK109_01495"
     tRNA            1831..1904
                     /locus_tag="PK109_01495"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1865..1867,aa:Ile,seq:tat)
     gene            1931..2782
                     /locus_tag="PK109_01500"
     CDS             1931..2782
                     /locus_tag="PK109_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRH24248.1"
                     /translation="MKRLLIPALVLIAIILIAVLVSIYTRVPERHASWREDLALSPKV
                     TTLEDGSIQIENVRNWTYGSTTIVSKGWSEVEVNPKDVTRVWFMLEPFPYWDAIGHTY
                     LTFEFSDGTALSFSIEAHMEKDEKYSAFAGLWNEYELAYTWGTERDFATRRLLYLQHD
                     LRMYPLALTPEVAQELFVRFTKETVALQETPRFYNTLTENCTNTLAYMVNDLMPGALP
                     PDLSWYLTGRSDSYLMKHGIITEVSDSEQITQARYDLTPFRSDIARAATSSPAVFSRT
                     LRSLLAP"
     gene            complement(2779..3597)
                     /locus_tag="PK109_01505"
     CDS             complement(2779..3597)
                     /locus_tag="PK109_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24249.1"
                     /translation="MLKKVFTIFLYLFAAIGLFLVAGYAAVRLGLTNETGIIDVQRES
                     FLGGMVPTGDAIVTDWHVSEEWLVLDGAIRRDEAVVKRAAADAGVSPRLILANLVAEQ
                     LRLFFTEREAYKQFFYPLKILGPQTQFSWGVMGMKEDTALQVEANLKNTDSPFYLGPQ
                     YEHALDFAATTTDIKTARFVRMTDQHDHYWSYLYAGLYMKQIEAQWRKAGYALDGNVA
                     VISTLYNIGFANSVPKATPSVGGAAIQIGDKTYSFGGLAVDFYESNLLIDIFPR"
     gene            3703..4692
                     /locus_tag="PK109_01510"
     CDS             3703..4692
                     /locus_tag="PK109_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRH24250.1"
                     /translation="MSLLVVTILIVLAFAALFASVEAAIFCTSFPRAQVLKRQKKPGA
                     VALVAIREHMSRPVTTLVIGTNITTIVGSLLIGHVATEAFGDSALGIITAVVTFLIIV
                     FGEIFPKVMGEKFSEPIALFAARPLLLLTNLLTPFIWIIEKTTDRFTDHTRKIVSEEE
                     LKILSEIGSREGSIERDEEELIRRVFTLNDVTARQIMTPWKVVDTLKADAAVEDTVRI
                     LYEKPYSRYPVVDADGMVVGTCQTKDLLIALAQDRGQEKVASFMARSRFVPEDKRVDH
                     LIASFLASRRHMFIVKDTGGKQVGVVTFEDALEQLVGEIVDETDEVVDLRKGV"
     gene            complement(4695..6848)
                     /gene="recG"
                     /locus_tag="PK109_01515"
     CDS             complement(4695..6848)
                     /gene="recG"
                     /locus_tag="PK109_01515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRH24251.1"
                     /translation="MKPTDFIGEHFKFLKPEQKKALTRLGVRTIRDLLYHFPARYESS
                     EGVRTIGDLVVGETATLYGHLKGAKAKKTWKTKMPSAEAYLEDGSGRVKVMWFRQPYM
                     AKMTEKMGLVKITGKVAGTPEKPYITNPAVEKAESVPAISHESLFGGDEPVMFAVYPE
                     TKGISSLWFRHAIERTMRSLVQDEIDDIIPESLRAEYSLPKVATALVWMHAPKKASDA
                     AAARKRFAFEEVFSIQLAVQKARAESAHKVAPALTVDRSVVADFIKDFPFTPTKAQQD
                     AIASILEDFEKPHPMMRLLEGDVGSGKTAVAAAVAYAVAHARDKGSASRHLQVAYMVP
                     TEILAKQHFASFIEFFKKHHLSIGLITGKECYKFPSKVRGEMATRISKAQLSKWVANG
                     EIPLVIGTHALIQKGVQFRDLAFAIIDEQHRFGKVQRKSLARKDGKEPHLLSMTATPI
                     PRTLALTVYGDLDLTVLDQMPLGRKPVITEVVTEATRKKAYARIHEELAAGRQAYVIC
                     PRIDEPDPAKEFALQAKSAKSEQERLQNSEFTQYRVGLLHSKMTPAEKEEEMRKFVRN
                     ESQVLVATSVVEVGVNVPNATVILIEGAERFGLAQLHQLRGRVIRSNDQAYCFLLSES
                     KGDVTVARLKALTTAKNGFELAEADLALRGTGELYGPRQWGVSDIAMEALTNLKMVEA
                     ARSAARHIIEQDPTLTEHPLLAKRITLLSENLYGE"
BASE COUNT         1677 a         1799 c         1807 g         1695 t
ORIGIN      
        1 cagcaatcat cttcgtcgac gagattgacg ccgtaggacg cgtacgtggg gcaggggtag
       61 ggggcggtaa cgatgagcgt gagcagacct taaaccaaat actcgttgag atggatggct
      121 ttgacccgaa cgagaaagtc atcgttatgg ctgctacgaa ccgtcctgac gtacttgatc
      181 cggcactcct tcgtcctgga cgctttgatc gtcgcgtcac catcgattta ccagacagga
      241 gtgatcgtga agcgatactc aaggtgcatg cgcgcaagaa gccccttggt ccagacgtta
      301 cgcttaatgt catcgctgag cgcacgcctg ggttctcagg cgcagagctc cattcgctta
      361 tgaacgaagg cgctattctt gctgcacgcg agaatcgtaa agaaataacg cagtacgatc
      421 tcattcgttc gattgagaaa gtgatgctcg gtcccgagcg taagagtcat ctcctttcaa
      481 agaaagagaa gaaggttacg gcataccacg aggcgggtca tgcgctggtt tcttctgtac
      541 tcccgcatgc tgatccggtg cacaagattt ccatcattag ccgtgggcgc gctgccggct
      601 acaccctgaa gcttccgttt gatgataaga agatgcagtc taaaaagcag ttcaaggacg
      661 acattgcagc aaccttgggt gggtatgtgg cagaagaaat gattttcggc gacatcacta
      721 caggccctag taacgacctc gcggtcttga ccgcacttgc tcgtgacatg gttgcccgct
      781 acggcatgtc tgacgttata ggcccggttg cgttctccgc agagcgtaac tacgccggag
      841 agacaacgta ctcacaagac attgcgggca agatagacac tgaagtgtcc cgcatcatcg
      901 ctgaagcgcg caccaaggca acggacgtac tcaccaacca caagaaagcg cttgatgcta
      961 tcgctgagac cttggtagaa gtagaaacgc tcgagcgtga cgcatttgag aaactcctca
     1021 tcgccaatgg catcacgcct aagaagcgag aagaagacga aaccccggtt accgtcacag
     1081 actagttaaa agttgtatag atgcagtgga ttaccgcttg atagcggatt ttacaatgga
     1141 catatggcca agtacgagtt acatgatcgt gcgatacata tgcgtaagct cgggatgagc
     1201 tacacgcaga taaaagacga actcggggtc agtaaaagta cgctgagtgg gtggcttcag
     1261 cactacccgc tctctcctga gcgacttaag gaactccgta ccaacactga gcgacagatt
     1321 gagcggattc gtgatactaa aagtcgtaaa cgatcagcgc gtctcgcagc agtacgtgaa
     1381 caagttgaaa gaggtattag tcacctatca gatcgagaaa tattgatcgc tggtctgttc
     1441 ttatattggg gagagggcgg taagacgaaa ccgtatacga caacgatatc gaacacagac
     1501 cctgctatgc ttcttttctt catacactgg cttgaattgc ttggagtaga tagaaatcgc
     1561 attcgagcat atgtgcatct ttacgcggat atggatgcgg aaaaagaagt gaaatattgg
     1621 tcaacgacac ttaagctatc taagaatgcg ttcagaaaac cgcatataaa aaattcaaat
     1681 cgttgcgagc ttacctataa acagagattt tctcacggta cgtgcaatat cattttcgat
     1741 aatcgtgata ccgcagaata tgtatctcaa gcactggaag tcattcgtgc aagatttgct
     1801 cagggtatca atccatgatt ccatacagga gcgcccttag ctcagttggt tagagcacct
     1861 agcttatacc taggcggtcc ttggttcgaa tccaagaggg cgcacatttt ttggtgctac
     1921 agtatcgttc atgaagcgac tcctaatacc ggcactggtt ctaattgcta ttattctcat
     1981 tgcagtgctg gtatcgatat acacacgtgt tcccgagcgc catgcttcat ggcgggaaga
     2041 tctcgctctc tcaccaaaag tcaccactct tgaggacggt tctattcaga tagagaacgt
     2101 acgtaactgg acgtatggat caacgaccat agtatcgaag gggtggagcg aagtagaagt
     2161 gaatccgaaa gacgtgacgc gcgtgtggtt catgctggaa ccatttccgt actgggacgc
     2221 gatagggcac acctatctca cctttgagtt ttctgacggc acagcgcttt cattctctat
     2281 cgaggcgcac atggagaaag atgagaagta ctctgcattt gccggactct ggaatgagta
     2341 cgagctggcg tatacatggg gtactgaacg cgactttgcg acgcgtcgtc tcctttatct
     2401 tcagcacgat cttcgcatgt atccgttagc acttacgcct gaggtagcgc aggagttgtt
     2461 tgtacgcttc accaaagaaa ccgtagcgct tcaagaaact ccgcgcttct ataacactct
     2521 taccgagaac tgcacgaaca cgctcgccta catggtgaat gaccttatgc caggcgcgct
     2581 cccgcctgac ctctcttggt acctgacggg gcgttccgat tcttacctca tgaagcatgg
     2641 gatcattaca gaagtaagcg attcagaaca aattactcaa gcgcgttatg acctgacgcc
     2701 attcagaagc gatatagcgc gtgccgccac gagttctcct gcagtcttta gtcgcacgct
     2761 ccgctcactt cttgctccct agcgcgggaa gatatcgata agaaggtttg attcgtagaa
     2821 atcaacggca agaccgccga agctgtacgt cttgtctcca atttgtattg ctgcaccgcc
     2881 tacagaagga gttgctttcg gtacggagtt agcaaagccg atgttataca aggtcgaaat
     2941 gacagcgacg ttcccgtcga gagcgtagcc cgccttacgc cactgcgctt ctatctgttt
     3001 catatagaga ccagcgtaga gatagctcca gtagtggtca tgctgatcgg tcatgcgcac
     3061 aaagcgcgcc gtctttatgt cagtcgtggt agcggcaaaa tcaagcgcgt gctcgtactg
     3121 aggtcctaaa tagaaaggag agtcagtatt cttaaggttc gcttcaacct gtagagcggt
     3181 atcttctttc atgcccatca cgccccacga gaactgcgtc tgaggtccca gaatcttcag
     3241 tggatagaag aactgcttgt atgcttctcg ctcggtgaaa aagagacgca gctgctctgc
     3301 gacaaggttc gcgaggataa ggcgaggaga cacgcctgca tctgccgctg cacgcttcac
     3361 gaccgcttcg tcgcgacgta tggccccatc aagtacgagc cactcttcgc tcacgtgcca
     3421 gtcggtgacg atggcgtccc ctgtaggcac catgccgccc aagaagcttt cccgttgtac
     3481 atcgatgatg ccggtttcgt tcgtgagccc gaggcgcact gccgcatatc ctgccaccaa
     3541 gaaaaggcca atggcagcaa agagatagag gaagatggta aagacttttt taagcatgtg
     3601 aatcgttttt tcgagtatgc cggtcagaac gcttggacgc aactctagag tactcgttcc
     3661 tgcatgaaat agagatgggg tatactcggc tctatcgggc atatgtctct cctcgtggtc
     3721 accatcctta tcgtgctggc gtttgccgca ctctttgcgt cagttgaagc agctatattc
     3781 tgtacgtcgt ttccgcgtgc ccaggtcctg aagcgccaga agaagccagg cgctgtagcg
     3841 ctcgtggcga tacgcgagca catgagccgg cccgtcacca cgctcgtgat tggtaccaat
     3901 atcacaacca tcgtcggaag cctcctcatc ggtcacgtcg ctaccgaagc attcggcgat
     3961 agtgcgcttg gcatcatcac cgcagtggta acgttcctca tcattgtgtt cggggagata
     4021 ttcccaaaag tgatgggaga gaagttttct gaaccaattg cgctctttgc agcgcgccca
     4081 ctcctacttc tcacgaacct gctcacgcct tttatttgga taattgaaaa gacgaccgat
     4141 cgattcacgg accacacgcg aaagatcgtg tcagaagaag aactcaaaat actttcagag
     4201 atagggagtc gtgaaggctc catagagcgt gacgaggaag aactcatccg ccgcgtcttt
     4261 acgcttaacg atgtgaccgc gcgtcagatc atgacgccct ggaaggtggt ggataccctt
     4321 aaggccgatg ctgccgttga agacacggtg cgtatcctct acgaaaaacc atactcacgc
     4381 tacccggtgg tggacgctga cgggatggtg gtaggtacct gccagaccaa agatcttctc
     4441 attgcgctcg ctcaagaccg tggccaagag aaagttgcaa gcttcatggc gcgctctcgc
     4501 tttgttcctg aagataagcg cgtcgatcat ttgattgctt cgtttcttgc gagcaggcgt
     4561 cacatgttca tcgtgaaaga cacaggagga aaacaagtgg gtgtggtgac ctttgaagac
     4621 gcgcttgagc agttggtagg ggagatagtg gacgagacgg atgaagtcgt ggatctacga
     4681 aaaggagtct agtcctattc accgtacaga ttctccgaaa gaagcgttat ccgttttgcg
     4741 agtagcggat gctcggtaag ggtagggtct tgttcgataa tatggcgcgc tgcacttctg
     4801 gcagcctcta ccatctttaa gttggtgagc gcttccattg caatatctga tacgccccac
     4861 tgacgtggcc catataactc tccggtaccg cgaagcgcta agtcagcttc tgctaactca
     4921 aacccgttct ttgcggtggt gagcgctttt aatcgagcga cagtgacatc gcccttactc
     4981 tctgagagaa ggaaacagta cgcctgatcg ttggaacgga tgacgcgccc gcgtaactga
     5041 tggagctgcg ccagaccaaa gcgctccgcg ccttcaatta atatgacggt ggcgttcgga
     5101 acgttcacgc ccacttcaac cacagacgtt gcaacaagca cctggctctc atttctcaca
     5161 aacttacgca tctcttcttc tttttctgcc ggagtcatct tgctatggag aagccccact
     5221 ctgtactgcg tgaactcact attttgaagg cgttcttgtt ctgatttagc ggactttgcc
     5281 tgaagtgcga attctttcgc aggatccggt tcatcaatgc gcggacatat cacatacgct
     5341 tggcgtcctg cagcaagctc ttcatggatg cgagcgtatg cttttttgcg tgtggcttcg
     5401 gttactactt ccgttatgac gggcttacgc ccaagcggca tctgatccag tacggtcaaa
     5461 tcaaggtcac cgtatacggt gagcgcgagt gtgcggggga taggggtcgc ggtcatcgaa
     5521 agaaggtgcg gctctttgcc gtctttgcgt gcgagcgatt tgcgctgcac tttaccaaag
     5581 cggtgctgct cgtcgatgat ggcaaacgca agatcacgga actgcacgcc tttctggatg
     5641 agggcgtggg taccaatgac gagtggaatc tctccgttag cgacccactt tgagagctgc
     5701 gccttggaaa tcctggttgc catctcgcca cggacttttg atgggaactt gtagcactcc
     5761 ttacccgtaa tgagcccaat ggagaggtgg tgcttcttaa aaaactcaat aaaggaagcg
     5821 aaatgctgct tcgcgagtat ttcagttggg accatgtacg ccacctggag atggcgcgat
     5881 gcactccctt tatcacgtgc gtgagcgacg gcgtacgcta ccgctgccgc aaccgccgtc
     5941 tttccgctcc ctacatctcc ttctaaaagg cgcatcatcg ggtgtggttt ctcaaagtct
     6001 tcgagtatgg aagcgatagc gtcctgctgc gctttggtag gggtaaacgg aaagtcctta
     6061 ataaaatcag cgaccacact gcggtccact gtgagcgccg gtgctacttt atgcgctgac
     6121 tccgcccgcg ccttctgcac cgcaagctga atagagaaca cctcttcaaa cgcaaagcgc
     6181 ttacgtgccg cagcagcgtc cgacgccttt ttaggtgcgt gcatccatac aagcgcggtg
     6241 gctacctttg gaagagaata ctcagcacgg agcgattctg gaatgatgtc gtcaatctcg
     6301 tcctgtacta acgagcgcat cgtgcgctca atggcatgcc ggaaccataa ggacgaaata
     6361 cctttcgtct cagggtatac ggcaaacatc accggctcat ctcctccaaa gagtgattca
     6421 tgcgatatgg ccggtactga ctctgctttc tcaacggcag ggttcgtgat gtacggcttc
     6481 tcgggagtgc cggctacttt ccccgtaatc ttcacgagcc ccatcttctc ggtcatcttc
     6541 gccatatagg gctgacggaa ccacatcacc ttcacgcgtc ccgagccgtc ttcaaggtac
     6601 gcttctgcgc tcggcatctt cgtcttccag gttttcttgg cttttgcacc tttcaagtgg
     6661 ccgtacagag ttgccgtctc gccaactact aagtcaccta tggtgcgcac cccttcggag
     6721 gattcatagc gcgcggggaa gtggtagaga aggtcccgaa tggtgcgcac gccaagacgg
     6781 gtgagcgctt tcttctgctc gggctttaga aacttaaaat gctcgccaat gaaatcggtg
     6841 ggcttcatgc tcgttcagta tacgcttcgt ggttgttcat gctgcgcaca tgtcatacac
     6901 aacatactta tgcgtctaca gtggtataaa gaatatatga aacaactcac cgccggcatt
     6961 cttgcactcg cttttttc
//
LOCUS       DAORIN010000023         6951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_1982_length_6951_cov_5.454611, whole genome shotgun sequence.
ACCESSION   DAORIN010000023 DAORIN010000000
VERSION     DAORIN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1982_length_6951_cov_5.454611"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PK109_01520"
     CDS             <1..484
                     /locus_tag="PK109_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRH24252.1"
                     /translation="EAEKSRQKDVFARHIQLAIQTGKPLMIHARPSKGTMDAYEDAYE
                     MLAEAKAKHPALSMNMHFYVGDVPMTEKLLTLGATFSYTAVLTFTHDYDDVVQAIPLQ
                     NLLSETDAPYVAPAPNRGKRNIPLAARDVVAAIARIRGEDEEVVRAAILENARRVFSI
                     "
     gene            complement(535..882)
                     /locus_tag="PK109_01525"
     CDS             complement(535..882)
                     /locus_tag="PK109_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24253.1"
                     /translation="MANNAVRLVQGFQSDAQKIGLTFGSVGDDNAPQDPTPFVIDTTT
                     KLIPLKSGGYLDPEQVKCFALAEKDGYTLIVVCIKSIEGPRDHYVLGKTLDTPAADCW
                     VTRANDCFSRVGA"
     gene            994..1473
                     /locus_tag="PK109_01530"
     CDS             994..1473
                     /locus_tag="PK109_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24254.1"
                     /translation="MAKKDAAASLPEEMDADHAELRVYELGFHIDPELGTEEVKKTYQ
                     AIRTLAEAGSIVAEGEPTKIPLAYTISRSETSGRRDFDSSFFAWIAYEATGEGHNKVL
                     EAAGADKRIFRFLDIRTDKEAAKHSAEMHEIMAKMEPVETEEEVSDAELDAALKEVA"
     gene            1620..2090
                     /gene="ssb"
                     /locus_tag="PK109_01535"
     CDS             1620..2090
                     /gene="ssb"
                     /locus_tag="PK109_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRH24255.1"
                     /translation="MYLNKVQLYGNLTRDPEVKALPSGQNVANFSVATNRVFKDKNGA
                     KQEQTEFHNVVAFGRTADVIGQYLKKGRPVYIEGRIQTRSWEKDGAKQYRTEIVAETF
                     QFGANAGGGQGGGGGYDGGSRSSSPDEGAQSAPADSGDAIQYPDEDINPEDIPF"
     gene            2136..2342
                     /gene="rpsR"
                     /locus_tag="PK109_01540"
     CDS             2136..2342
                     /gene="rpsR"
                     /locus_tag="PK109_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRH24256.1"
                     /translation="MAFQAEREEIENKKFIDYKDVVYLRQFMNPHAKILSKKRTGIPA
                     DKQRQIALAIKRARYMALLPYLAA"
     gene            complement(2386..2961)
                     /locus_tag="PK109_01545"
     CDS             complement(2386..2961)
                     /locus_tag="PK109_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24257.1"
                     /translation="MAILSYNEITLKKVIVYEGQPYKVLSSHVFRMQQRKPVNQTKLQ
                     HLVSGRVIEISFHQNESVPEADIERMEATYLYTNRGESWFCEVGNPKNRFSFPEEAVH
                     DKVQWLVQNSPVECLVFEEKPVTLEIPIKMDLKVTEAPPAVKGDTATGGNKQVTLESG
                     ATVLTPLFINPGDVLRINTETGEYVERMEKA"
     gene            3075..3311
                     /locus_tag="PK109_01550"
     CDS             3075..3311
                     /locus_tag="PK109_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24258.1"
                     /translation="MHIKVNVPTAEVGGASEVFAPVGTIQRLDEGPERTILTYEVTDR
                     SAFVAGARDLGLQFEDQSHDLPDRGEDYRSEYGG"
     gene            complement(3365..4369)
                     /gene="recA"
                     /locus_tag="PK109_01555"
     CDS             complement(3365..4369)
                     /gene="recA"
                     /locus_tag="PK109_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRH24259.1"
                     /translation="MSGDAPATDKSKSIELALKEIRTKFGDESIMTYGAGKVAAIPSV
                     STGSIGLDAALGIGGFPRGRIIEIYGPESSGKTTLALHAIAEAQKKGGVCAFVDAEHA
                     LDPEYARKIGVKLPELLISQPDTGEQALEIVESLVRSNSVDIVVVDSVAALTPKDEIE
                     GDMGSAQMGKQARLMSQALRKLTAITAKSKTIIIFINQIRMQIGMMFGNPETTTGGKA
                     LKFYTSVRLDIRRIAQIKKGEEVVGGRVRVKVVKNKVAAPFKTTEFDLLYNEGISREG
                     EILALGEKYDLIQKSGSSYKYGEVSLGRGYDASRTFLRENKPIATEILKQIKEKLAAA
                     "
     gene            complement(4433..4759)
                     /locus_tag="PK109_01560"
     CDS             complement(4433..4759)
                     /locus_tag="PK109_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24260.1"
                     /translation="MVQKTAAAIVLILLLGYGAVKAFPLVRGPHLQIDTPTNYTTSED
                     GFTTISGVAHNTEALFLNGGPLLIDPEGRFYETLLLPSGGAILTLTANDRFGRTVTER
                     LQIYVP"
     gene            complement(4763..>6951)
                     /locus_tag="PK109_01565"
     CDS             complement(4763..>6951)
                     /locus_tag="PK109_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRH24261.1"
                     /translation="QVGAKIHDMKGKKSKKEQSSQGVMRWVLAIVLIALGLILLLAAF
                     GSAGFAGNALFTFTRDIFGLAAFILPLCIVAIGVMVIMKRSLVSPLVAAGGALIVISI
                     LALLGIMSQTLGGVAGSFVGHLASDLFGIGGAVILLLALSLLGLIIATDIVVLMNKLM
                     EAANKTGEALSAVKLPEIGKKGEVSEDDDEVEEPVAAPVAAAPDREPTVTVFNDNVDK
                     SGTAIPNFDAEYNPPPLSLLGEDKGKATGGDIKANANIIKRTFQNFGISIEMDEVSVG
                     PTVTRYAIKPAEGVRLSKVVSLQSNLELALAAHPVRIEAPIPGKSLVGIEVPNSAKAM
                     VGLGGVLSSPEWSSSEKPLLGAVGRDIAGTPHYVNIAKMPHGLVAGATGSGKSVAIHA
                     IITSLLYRNGPNQLRFIMIDPKRVELTAYNGIPHLLTPVITDPKKCIMALKWAAKEMD
                     RRYNILETEQVRDIDSYHKTVVEPAKKRKSGDMPESMPYIVVVIDELADIMQSYPREL
                     EAGIVRLAQMSRAVGIHLVLSTQRPSVNVITGLIKANVPTRMALQVASQIDSRTILDS
                     GGAESLLGAGDMLYLSSDMQKPVRLQTAYISENEVKKVVSYIKQHNAGTLGGLDLGGA
                     SGTSSSNGGQPIEMNDAIMLSGGGDDDVDDDLYMEAKAAVEEAGRASTSYLQRKLRIG
                     YSRAARLMDILEEKGVIGAADGSRPRDVLSRGAAATTPAADDEQQF"
BASE COUNT         1637 a         1830 c         1886 g         1598 t
ORIGIN      
        1 agaagcggag aagtcacgac agaaggacgt gttcgcgcgt catatacaac tcgcgataca
       61 aacaggaaag ccgctcatga ttcatgcacg tccatcaaaa gggactatgg atgcctatga
      121 agacgcgtac gagatgcttg cagaagcaaa agcgaagcac ccagcactct ccatgaacat
      181 gcacttttat gtaggggacg ttccaatgac cgagaaactt cttacgcttg gtgcgacatt
      241 ctcctatacc gcagtgctca cctttactca tgactatgac gacgtcgtgc aggccattcc
      301 acttcagaac ctgctttctg agacggacgc accctatgtc gctcccgctc cgaaccgtgg
      361 gaaaagaaat attccccttg ctgcacgtga cgttgttgcc gctatcgctc gtatcagggg
      421 agaggatgag gaagtagtac gagccgcgat actagagaac gcacgccgcg tcttttcaat
      481 ataaagatgg aaggccgctg cgagtaattc gcaaacggcc ttcttcgcct tatttcaggc
      541 gccgacacga ctgaagcagt cgtttgcgcg cgtgacccag caatctgctg ccggcgtgtc
      601 caaggttttg ccgagtacat agtggtctct cggtccctca atgctcttga tgcagacgac
      661 gatcagggta taaccgtctt tttccgcaag cgcaaagcat ttcacttgtt ctgggtcgag
      721 atatccgccg cttttaagtg ggatgagttt cgtcgtagtg tcgatgacaa aaggcgtggg
      781 gtcttgaggt gcgttgtcgt ctccgactga accgaaggtc agtccgatct tttgagcgtc
      841 gctttggaag ccctgcacca gccgcactgc attgttagcc atggcctact ccttccagcg
      901 ctctatgcgc gtcaccagaa actacgcctg acctgtacca gtgtcaaact accggttgcc
      961 cgctctaaaa ccccatgcta ggatggcccc actatggcta agaaagacgc agcggcttca
     1021 ctgcccgaag aaatggatgc agatcacgcg gaactccgcg tgtacgagct tgggttccat
     1081 attgaccctg aactcggaac ggaagaggta aagaagacct accaggctat ccgcactctt
     1141 gcggaggcag gctctattgt ggcggaaggt gagccaacca agatcccgct cgcatacact
     1201 atctctcgct ctgagacttc tggacgccgc gacttcgact caagcttctt tgcatggatt
     1261 gcatacgaag caacgggtga aggacataac aaagtgcttg aggcagcagg agctgacaag
     1321 cgcatcttcc gtttccttga tatccgcacg gataaggagg cagcaaagca ctcagctgag
     1381 atgcacgaga ttatggcgaa gatggagcca gtagagaccg aagaggaggt atcggatgca
     1441 gaactcgatg ctgccctcaa ggaggtagcg taaccgctcc tcatacggta gcgcaaaaag
     1501 gtccttcagg gtcctttttg cttgcatccg ttgaccggag gtatactggc aggagcgtgt
     1561 cgggaactac tgtttaggta gaccggcctc gcttgaataa gactaaaaaa tcactgcata
     1621 tgtatttgaa caaagttcag ttatacggga accttacgcg cgatcctgaa gtgaaggcac
     1681 tgccttctgg tcagaacgtt gcgaacttta gtgttgccac caaccgtgtc tttaaagaca
     1741 agaacggtgc caagcaggaa cagacggaat tccataacgt cgttgccttt ggacgcactg
     1801 cagacgttat cggtcagtac ctaaagaagg gacgcccggt ttatatagaa ggacgtatcc
     1861 aaacacgttc gtgggagaaa gacggggcga agcaataccg caccgaaata gtggcagaga
     1921 ccttccagtt cggcgctaat gccggaggtg gccagggtgg gggaggtggc tatgatggcg
     1981 gcagtcgctc atcatctccg gacgagggcg cccagagcgc tcctgctgac tcaggggatg
     2041 ctatccagta tccggacgag gatattaacc ccgaggacat cccgttctaa ggctgttttg
     2101 acagcagaca cgtactaagg taacttaggg gaactatggc atttcaagca gaacgcgagg
     2161 aaatcgagaa caagaagttt attgactaca aggatgtggt ctaccttcgt cagttcatga
     2221 atccgcacgc aaagattctc tcaaagaagc gcaccggtat tccggctgac aagcagcgcc
     2281 agatcgctct tgcgattaag cgcgctcgtt atatggcgct tctcccgtac ctcgctgcat
     2341 aagcagctag gtaaagaaaa agccccgcga tgcggggctt tttctttatg ccttctccat
     2401 tcgctctacg tattcgcccg tctcagtatt gatgcgtagt acatcgcctg ggttaatgaa
     2461 aaggggagtg aggaccgtag cgccagattc aagcgttacc tgcttgttac cgccggtagc
     2521 ggtgtctccc ttcactgctg gaggtgcttc cgttaccttc aagtccatct tgatagggat
     2581 ttcaagcgtg accggtttct cttcaaacac gaggcactcc accggagaat tctgaacgag
     2641 ccactgcact ttgtcgtgta ccgcttcctc agggaaggag aagcgattct ttgggttacc
     2701 tacctcacag aaccaggact cgccgcggtt cgtgtagagg tacgttgctt ccatacgctc
     2761 aatgtctgcc tcagggacgc tctcgttctg atggaaagag atttcgatga cacgtccaga
     2821 tacaagatgc tggagcttgg tctgattcac cggcttacgc tgctgcatgc ggaacacgtg
     2881 cgaagaaagg accttgtacg gctggccttc gtatacgatg accttcttaa gtgtaatttc
     2941 gttgtatgag aggattgcca tagcagttca ataaaaaatg gccgagggcc gtaatggggg
     3001 aatgatactt gaataccgac tattttcaag cgtagagtcc gtgctatacc tataaggttc
     3061 ggaggaatat agccatgcac atcaaagtta acgtccctac cgctgaggta gggggcgcat
     3121 ccgaagtatt tgccccagta gggacaatcc aacggctcga tgagggccct gaacggacta
     3181 tcctcacgta cgaggttact gatcgctcag cgtttgttgc aggcgcccga gacctcggtc
     3241 ttcagttcga ggaccaaagc cacgatcttc cagatcgcgg cgaggactac cggagcgaat
     3301 acggcggata aacgatcttt ccgcctagaa cccgcgctgt cccaaaaggg gagcgcgggt
     3361 tttgctatgc tgctgcgagc ttctccttta tctgcttcaa gatttcagta gcgataggtt
     3421 tattctccct gaggaacgtg cgcgacgcgt catacccgcg gccaagcgac acctcaccat
     3481 acttatagga cgaacctgac ttctggatga ggtcgtactt ctcaccgagc gcgagaattt
     3541 ctccctcacg gctaatacct tcgttataga gaaggtcgaa ctcagtcgtc ttgaatgggg
     3601 cagcgacttt attcttcact accttcacgc gcacacgccc tcccaccacc tcttcacctt
     3661 ttttaatctg cgcgatgcga cggatatcaa ggcgcactga ggtgtagaac ttaagggcct
     3721 taccgccggt agtggtctca gggttaccaa acatcatgcc tatttgcatg cgtatctggt
     3781 taatgaagat aatgatggtc ttcgactttg cggtgatggc ggtgagcttg cggagcgcct
     3841 gactcatcaa gcgtgcctgt ttacccatct gtgccgaacc catatcacct tcgatctcat
     3901 cctttggggt gagggcagcc actgagtcca cgacgacaat gtcgacggag ttggaacgca
     3961 ccaaggactc cacgatttca agcgcctgtt cgccggtgtc cggctgagaa atgagaagct
     4021 cgggaagctt cacgccaatc ttgcgagcgt actcaggatc aagtgcgtgt tcagcatcca
     4081 cgaacgcaca cacacctcct ttcttctgtg cttctgcaat agcatgaagc gcaagagtgg
     4141 tcttaccaga agactctgga ccatatatct cgatgatgcg tccgcgaggg aagcctccga
     4201 taccgagcgc ggcatcgagt cctatagagc ccgtggagac ggacgggatt gcggcaacct
     4261 tgccggcgcc atatgtcatg atggactcgt cgccgaactt cgtgcgaatc tctttgagcg
     4321 cgagctcaat cgacttactt ttatcagtcg caggagcgtc gccgctcatt tcaatgtctg
     4381 cagtgtctac tggtttcttc gtggtctttt tctttggtgc catatgaatt gattagggaa
     4441 cgtaaatctg aaggcgttcg gtaacggttc tcccgaagcg gtcatttgcg gtcagcgtaa
     4501 gtatagctcc cccggaaggg aggaggaggg tctcgtaaaa gcgtccttcc gggtctatca
     4561 ataagggccc tccattcaag aataaggcct cagtattatg cgccacgcct gaaatggtcg
     4621 tgaagccgtc ttctgaagtt gtgtagttgg tgggggtatc tatttggagg tgcgggccgc
     4681 gcacgagcgg gaacgccttc accgcgccat acccaagaag gagaatgagg acgatggcag
     4741 cggcagtctt ctgaaccatt gcttagaact gctgctcgtc gtcagcggca ggagtggttg
     4801 ctgcagctcc gcgcgaaagt acatcacggg gacgtgatcc atcagcggca ccaatcacgc
     4861 ccttctcttc aaggatatcc atgagtcgtg cagcgcgtga gtagccgatg cgaagtttgc
     4921 gctggaggta tgaggttgac gcgcgtcctg cttcttctac cgccgccttc gcttccatat
     4981 agaggtcatc atccacatcg tcatcaccac ctccagaaag catgatggcg tcattcattt
     5041 caataggctg gccaccgttg ctgctactcg tgcctgacgc acctcccaag tcgagaccgc
     5101 ctaacgtacc agcattgtgc tgcttaatgt agctcaccac cttcttcact tcgttctcgc
     5161 taatgtaggc ggtctgaaga cgtacgggct tctgcatatc gcttgagaga tagagcatgt
     5221 cgccggcacc aaggagagac tctgcacctc ctgagtcgag aatagtgcgc gagtcaattt
     5281 gggacgccac ctgaagcgcc atgcgggtag ggacgttggc cttaatgagg ccggtgatga
     5341 cgttaacgga agggcgctgg gtagagagga cgagatggat acccacggcg cgtgacatct
     5401 gtgcaaggcg tacgatgccg gcctcaagct cgcgggggta gctctgcata atatcggcaa
     5461 gctcgtcgat aacgacgacg atgtacggca tcgactctgg catatcaccg ctcttacgct
     5521 tctttgcagg ctctaccacg gtcttgtggt acgaatcgat atcgcgcacc tgttccgtct
     5581 caaggatgtt atagcggcga tccatttctt tagccgccca cttgagtgcc atgatgcact
     5641 tctttgggtc agtgatgact ggggtcagta agtggggaat accgttatac gccgtcaatt
     5701 ccacgcgctt cgggtcaatc atgatgaagc gcagctggtt cggaccattg cggtagagaa
     5761 gggaggtgat gatggcgtgt atagcaacag acttacccga gccggtcgcg cctgccacca
     5821 gtccgtgcgg catcttcgcg atgttcacgt agtgcggagt acccgcaatg tcgcgtccca
     5881 cggcaccaag aagcggtttc tcgcttgagc tccattcagg ggaagagaga acgcctccaa
     5941 ggcccaccat cgctttagcg gagttcggta cttcaatacc caccaacgat ttacccggga
     6001 taggagcttc aatacgaacg gggtgcgcgg caagggcaag ctcgaggttc gattggagcg
     6061 acacgacctt tgagaggcgg acgccttcag caggcttaat agcgtagcgg gtaacggtgg
     6121 ggccaaccga tacttcgtcc atctcaatgg aaataccgaa gttctggaag gtgcgcttga
     6181 tgatgttggc gtttgccttt atgtcgccac cggtggcctt acctttatct tcaccaagaa
     6241 gagagagggg aggggggttg tattcagcat cgaagtttgg aatcgccgta ccacttttat
     6301 caacgttatc gttgaacacc gttaccgtag gctcacggtc aggagcggca gcgacaggag
     6361 ctgccactgg ttcttccact tcgtcatcat cttcagacac ttctcctttc ttaccaatct
     6421 cgggaagctt cacggcgctc aatgcttcac ccgtcttatt tgccgcttcc atgagtttat
     6481 tcatgaggac gacgatatcg gtagcgatga tgagtccgag taaggagagc gcgagaagaa
     6541 gaatcaccgc tccaccaata ccaaagagat ctgaagcgag gtgtccgaca aaggaaccgg
     6601 cgacaccgcc caaggtctgc gacatgatgc cgaggagcgc cagtatagaa atgacgatga
     6661 gcgcgccgcc tgctgcaacg agaggggaca ctaagcttcg cttcatgatg accatgacgc
     6721 caatagcgac gatacaaaga ggaaggatga atgcggcaag tccgaagata tcgcgggtaa
     6781 acgtgaagag cgcgttacca gcaaacccag cactcccaaa tgcggcaagc aataagatga
     6841 ggccaagcgc gataagcacg atggctaata cccatcgcat cactccctgg gagctctgct
     6901 ccttctttga tttcttgccc ttcatgtcat gaattttagc acctacctgc c
//
LOCUS       DAORIN010000024         6796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2053_length_6796_cov_5.199377, whole genome shotgun sequence.
ACCESSION   DAORIN010000024 DAORIN010000000
VERSION     DAORIN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2053_length_6796_cov_5.199377"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK109_01570"
     CDS             complement(<1..278)
                     /locus_tag="PK109_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRH24262.1"
                     /translation="MIAKVQEKTKALQLRRQGYSYQDILKEVKVSKSSLSLWLNELPL
                     TNEEESFLKTRKDSNISRGRIRAAVSNRMRRVVRDGFITRDAKREFEE"
     gene            345..1808
                     /locus_tag="PK109_01575"
     CDS             345..1808
                     /locus_tag="PK109_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRH24263.1"
                     /translation="MKFSVPIEVSSVAITLRKGGFEAYLVGGCVRDLLIGRRPKDWDI
                     TTNAKPEEIQALFPDSFYENDFGTVGIKTESEDPTLSIIEVTPYRTESEYTDKRRPDK
                     VEFGQSLEEDLARRDFTINAIAYDESKGQIVDPYKGQKDIEAKVLRTVRDAEERFEED
                     GLRLMRAVRLVAELNFALDSDTAAAIQKKASNLAHISRERIRDELVRILQSDQPMMAL
                     VLAQQLGLLELISPKLVRGIGVEQNQAHSYDVFGHLMRAMQHAADKGWSLDLRLSALF
                     HDVSKPETRRWSKDKNDWTFHGHEVVGSRVTEKILHDLRFSRETIEKVVKLVRWHMFF
                     SDPDQITLSAVRRMIRNVGEENIWDLLNLRICDRIGTGRPKEQPFRFRKYKAMVEEAL
                     RDPISVAMLKTNGAHIMEKFHMKPGPRIGWTLNALLEDVLEDPKKNTLSYLDEKTEEL
                     LELTDAKLQTLGESGKKMREAKEEEELKKILDKHHVC"
     gene            complement(2136..2927)
                     /locus_tag="PK109_01580"
     CDS             complement(2136..2927)
                     /locus_tag="PK109_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRH24264.1"
                     /translation="MRIISWNVNGIRAVQKKGLFLPFLKKYKPDILCLQETKAQEGQA
                     EIDMPDYEEYWCSAEKKGYSGVAIFTKMTPNDVRYGLPEDLAEKYGLHTDGYGDPNKE
                     GRIIAADMFDFFVVSVYTPNAKDDLSRLPLRHKQWDPAFLEYMKRLEAEKPVIFCGDL
                     NVAHTEDDLARPKENRGVKGFTDEEREGIQAMIDSGFVDTFRMFTKGNGHYSWWSHFA
                     QARERNVGWRIDYVFASKKIATKIQKAEIHPEVFGSDHCPVSVVL"
     gene            complement(2946..3716)
                     /locus_tag="PK109_01585"
     CDS             complement(2946..3716)
                     /locus_tag="PK109_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRH24265.1"
                     /translation="MSEKTDEISDKDLLQQLESIGLTEKESRVYLALLPRHDVGSSKI
                     ISATGLHGQFVYDALERLEKLGLAKHVIANGRKKFSANPPLRLLSIVEEKRLAAQNIA
                     RQLQERYSGKHEQDFEVFQGETAFMMHQFDMLERVAQDQEAVAICGPTERFLATLGDG
                     ETDEFEKLRIKKGLRVRYIGMEPMRERLAEMKKWRKLWDYRIFPGQSTGLVDTDIWPD
                     NITFNIFGDPILSFTITNKAAADGYKEFFEGLWNVSKP"
     gene            4019..4231
                     /locus_tag="PK109_01590"
     CDS             4019..4231
                     /locus_tag="PK109_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HRH24266.1"
                     /translation="MTALAYIDVLSIDTLGLPSKTTRALQYLADIETIGELRRKTPKE
                     LRKIPCIGKKGVEDIIEVLRNFPPPE"
     gene            4281..5102
                     /locus_tag="PK109_01595"
     CDS             4281..5102
                     /locus_tag="PK109_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24267.1"
                     /translation="MSPEVFAPKLEKERLVHDLLTQTYSSVPFPLSHEEIDQAVGKYF
                     AFLTRSQEEKNAMLAKRPNDPVSDMGYVRVSGDASVIGSKDFKEYFHYHPEAEAVWAK
                     EAGEDVRVREFFDAAALIWQKADETGREVVRTLDAEFPGLYDAFFPADRPQERILRFL
                     KYDARGMGNFLARAHYDRGGCTLGIAESAPGLRIGKDEATLMPVVHKDRTALFFPAIH
                     FNQLSGGRVPAAWHDVVQASDDAVSEDAARWAVVYFMNAPAMPFPSEFDTHVPIK"
     gene            complement(5191..5955)
                     /locus_tag="PK109_01600"
     CDS             complement(5191..5955)
                     /locus_tag="PK109_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24268.1"
                     /translation="MSSILYRLIFLTSVFLLQSEWCRWCYTDDFGGASRPYALAVAVG
                     AFSLLVIRTTWTLRIAALGGALWYAFEVTDNLFGYSIAIGILGLLPLLTMHIVTSAHN
                     AGMASDSYDFGRFLGRIIRNTQRGRAWGQLQFDVTFALGCGGAVLTTFVPELSGLQVP
                     AAALTFTTFVLAAPLPGRGPVVAVLALMLWRELYAVAAWAANAVAATVVLTVQLAFAY
                     APHKVRRIAQRTHVMTVRLGRAYRKMNEEPGALRYT"
     gene            complement(6100..>6796)
                     /locus_tag="PK109_01605"
     CDS             complement(6100..>6796)
                     /locus_tag="PK109_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRH24269.1"
                     /translation="RRTQNYYLIALRAFLKFLRKREIPCISPERIELAKLPERSLDLI
                     TNVELERLIGSAKGANEKALRDRAILELLFSTGLRVSELCGLNSDIDLSRDELSVRGK
                     GDKVRVVFISPSAKEAVRDYLKKRKDMEEALFVNVSAVDKTSKKKPARLTPRAVQMMI
                     KTYAAKAGITKKVTPHVLRHSFATDLLSNGADIRSVQALLGHASINTTQIYTHVTDAH
                     LREVHKKFHGKNR"
BASE COUNT         1690 a         1691 c         1756 g         1659 t
ORIGIN      
        1 ttctcttcaa attccctttt agcgtctctc gtgatgaatc cgtctcgtac tactcgtcgc
       61 atacgatttg aaacagcagc cctgatacga ccacgactta tgttgctatc ctttcttgtt
      121 ttaagaaaag actcttcctc gtttgtaagc ggcagctcat ttaaccaaag cgataggctg
      181 ctttttgaaa ccttcacttc tttaagaata tcctgatagc tatagccctg ccggcgtaat
      241 tggagagctt ttgtcttctc ttgaacctta gcaatcatgg tatgtacata gtattacaca
      301 gttcgaatgt atgttttcca cagcaaatac actaaaataa cgatatgaag ttttcggttc
      361 ctattgaagt aagttcggta gccataactc ttagaaaggg tgggttcgaa gcgtatttag
      421 tcggcgggtg tgtgcgtgat ttgctgatag gccggagacc caaagactgg gacatcacta
      481 ccaacgcaaa gcctgaggaa atacaggcac tgttcccgga ttcgttctat gaaaacgatt
      541 ttggcaccgt cggcataaag actgagtctg aagatcctac cctttccatt attgaagtaa
      601 ctccctaccg caccgagtct gagtacacgg ataagcgccg tccggacaaa gtggagttcg
      661 gacaaagcct tgaggaagac cttgcccgcc gggactttac gataaacgcc attgcgtatg
      721 atgagtctaa aggacaaata gtagacccgt ataaaggaca aaaggacatt gaagctaagg
      781 tactacgtac tgtaagggac gcagaagaac gctttgagga ggacggatta cgccttatga
      841 gggccgtacg ccttgtggct gagcttaact ttgcccttga ttcagatact gcagccgcta
      901 ttcagaagaa agcatcaaac cttgctcaca tttcacgtga aagaatccgg gatgagctcg
      961 tgcgcattct tcagtcggac cagccaatga tggctcttgt gcttgcccag cagcttggcc
     1021 ttcttgagct tatctctccg aaactcgtgc gcggcatagg cgtagaacag aatcaggccc
     1081 atagctatga cgtatttggc cacctgatgc gcgccatgca gcacgctgca gataagggct
     1141 ggagcctgga tttacgtctc tctgccctct ttcatgacgt atctaagccg gagacacggc
     1201 gatggtctaa agataagaac gattggacgt tccatgggca tgaggtggtt ggttcacgtg
     1261 taacagaaaa gatcctccac gacttgaggt tttcacgtga aactatagag aaagtcgtaa
     1321 aactggtccg ctggcacatg ttcttctctg accctgacca aataaccctt tctgccgtcc
     1381 gtcgcatgat ccggaacgtt ggtgaagaaa atatatggga tctcttgaat ctccgtatct
     1441 gtgaccgtat aggtactggc aggcctaagg aacaaccgtt ccgcttccgt aaatacaagg
     1501 caatggtgga agaagcgctt cgtgacccta tctctgtcgc catgcttaag actaatggtg
     1561 cccatatcat ggagaagttc cacatgaaac caggcccccg cataggttgg accctgaacg
     1621 cccttctcga ggatgtcctt gaagatccga agaagaatac gcttagctat ttggacgaaa
     1681 agacggagga gcttcttgag ctaaccgacg caaagctcca gacgcttggg gaatctggta
     1741 agaagatgcg ggaagcgaag gaggaggaag agctaaaaaa gatcctggac aaacatcacg
     1801 tttgctaacg aaacggcccc gaaaggggcc tgtttcatgt gaaatgcaaa aaaccgtctt
     1861 gcctgttctg gtaccaaata gggggcgggc aattgattgg tggtaccaag gagatagcgg
     1921 cttgacggct ttttgcgtgt ggtcctttca cgtggaacct cgccttgctg aagataatat
     1981 ttggggatcg ccccattcgt tagcagggaa gagaaccgtt aaggactgtt tagaagaaaa
     2041 gagcgcgcaa agaactcaat agaactctat tgctcatact atgtccttta catcgagagt
     2101 aaaggacatg tatgtggata agtgtccttt atgtcctata agacaacaga aacagggcag
     2161 tggtctgaac caaaaacttc cggatgtatc tctgccttct gaatctttgt cgctatcttc
     2221 ttagaagcga aaacgtagtc gatgcgccat cctacgttac gctcacgtgc ctgagcaaaa
     2281 tgactccacc aggaatagtg accgttaccc ttggtgaaca tgcggaaggt gtccacaaag
     2341 cctgaatcaa tcattgcctg aatgccttcg cgctcctcgt cggtaaagcc tttcacgccg
     2401 cggttttctt tggggcgggc taggtcgtct tcggtatggg caacattgag gtcaccgcag
     2461 aagataacgg gtttttcggc ctcaagacgc ttcatatact caagaaacgc aggatcccat
     2521 tgtttatgac gaagcggcag gcgagagagg tcatcttttg cgttcggggt atatacggaa
     2581 accacgaaga aatcgaacat atcagccgca atgatgcgac cctctttgtt cgggtcacca
     2641 tacccgtccg tatggaggcc atatttctcc gccaagtctt cagggaggcc gtaccggaca
     2701 tcattcgggg tcatcttggt gaagatagcc accccgctat atcccttctt ttccgctgag
     2761 caccagtact cctcatagtc aggcatgtct atctctgcct ggccttcctg tgcctttgtt
     2821 tcctggagac agagaatgtc tggtttgtat ttctttagaa agggaaggaa gagacctttt
     2881 ttctgaacgg cgcgtatgcc atttacgttc caagaaataa ttcgcatgta cgcactatac
     2941 caaagctacg gtttagagac gttccacaag ccttcaaaga attctttata tccgtcagct
     3001 gctgctttgt tcgttatcgt gaaagaaagt atcgggtcac cgaaaatatt gaaggtgatg
     3061 ttatccggcc agatgtcagt atcgacaagg cccgtggatt ggccggggaa tatgcgatag
     3121 tcccatagtt tccgccattt cttcatctca gcgagccgtt cacgcatggg ttccataccg
     3181 atgtagcgaa ctcgtagccc ctttttaata cgaagcttct cgaactcatc ggtttcaccg
     3241 tctcctagcg tggcgaggaa gcgttccgta gggccacata tagcaaccgc ctcctgatcc
     3301 tgtgccacgc gctcgagcat atcgaattga tgcatcatga atgccgtttc accttgaaat
     3361 acctcaaagt cctgctcatg cttgccgcta tagcgttcct gaagctgacg ggcaatattc
     3421 tgcgctgcaa gacgtttctc ctcaacgata gaaaggaggc gaagtggggg attagcactg
     3481 aatttcttcc tcccattcgc aataacgtgc ttagcaaggc caagcttctc aagacgctca
     3541 agtgcgtcgt atacaaactg gccatggagg cccgtagcac tgatgatttt cgaagaccca
     3601 acgtcgtggc ggggaaggag cgctaggtat acgcgagact ccttctccgt tagccctatg
     3661 ctttcgagct gttgcagcaa atccttatcg gaaatttcat cagttttttc tgacatagtt
     3721 actaaatact ctatatttta aaggttttta tgtcaatatt tcatcatcat ataatattga
     3781 caataacaaa agccatgcta agatgttctt agacgcggga agggagtcga caccccggac
     3841 gcagcatgtc caatcgccga agatgagagc cggttgaggt tcaatagcgg cttcggaatc
     3901 gaaagggcca aaagagggtg gccaccctca cgtcgggggg cgagtgttgt tttcagacag
     3961 cattcgccct ctctattgag tccctgaatc attcggaggc tcatagagga gataggtgat
     4021 gactgctctt gcatatattg atgtcctttc catcgatact ctcggtcttc cgtccaaaac
     4081 cactcgcgcc cttcaatatc tcgcagatat tgagacgata ggggagttgc ggcgtaaaac
     4141 gccgaaggag ctgcgtaaga taccttgtat cggcaaaaag ggagtagaag acatcattga
     4201 agttcttcgg aacttcccgc cgcctgaata gggtggcggt ttcttttttt gtagatactc
     4261 tcgcgtgcta gcatggcgac atgtccccag aagttttcgc tcctaaactc gaaaaagaac
     4321 gattggtgca cgatctcctc acgcagacct actcctcagt accgtttcca ctctcgcacg
     4381 aggagataga ccaggcggta gggaagtact tcgcgtttct tacccgctcc caggaagaaa
     4441 agaatgcgat gctcgctaaa cgcccgaatg atccggtgtc agatatggga tatgtgcggg
     4501 tgagtggaga cgcaagcgtc ataggtagta aggactttaa ggaatacttc cactaccatc
     4561 ctgaagctga agcggtgtgg gcgaaagaag caggggagga tgttcgcgtt cgcgagtttt
     4621 tcgatgcggc agcactcata tggcagaagg cggatgagac ggggagggaa gtggtgcgca
     4681 cgcttgatgc agaattcccc ggcctctacg acgcattctt tcccgcagac cgtcctcaag
     4741 aacgtatcct tcgattcctt aagtacgatg cgcgcgggat gggaaacttc cttgcgcgcg
     4801 cccactatga ccggggcggg tgcacgctcg gcattgctga gagcgcgccg gggcttcgga
     4861 tagggaagga tgaagcgaca cttatgcctg tcgttcataa agaccggacc gcactcttct
     4921 ttcccgctat acacttcaac caactttcag gagggcgagt gccggcagcg tggcatgacg
     4981 tcgtacaagc atcagatgac gctgtgtcag aagatgctgc gcgctgggca gtcgtttatt
     5041 tcatgaatgc accggcgatg ccgtttccgt cagagtttga tacgcatgtg ccgataaaat
     5101 aatttcagtt gaacgtttat caaaaagtta aacgccccac cgactttctg tcgtgtggag
     5161 cgtttaagct atccccgctg ccgttcggcg ttaggtatag cgtagagcac ctggttcttc
     5221 gttcattttt ctgtaggctc gtcccaggcg gacggtcatc acgtgcgttc gctgagcgat
     5281 tcgccgcacc ttgtggggcg cgtacgcgaa tgccagttgc acggtgagga ccacggtcgc
     5341 agcgacggcg ttagctgccc aagcagcaac tgcgtagagt tcacgccaga gcatgagtgc
     5401 gagcactgct accaccggac ctcggcccgg gagcggtgct gccagcacaa atgtcgtgaa
     5461 cgtgagcgct gctgcaggca cttgaagccc tgaaagctct ggcacgaatg ttgtcagcac
     5521 cgcgccaccg catccaagag cgaacgtaac gtcgaattgc agctgtcccc atgcgcgtcc
     5581 gcgttgtgtg ttgcggatga tgcgcccgag gaacctaccg aagtcgtacg agtcactcgc
     5641 catgcctgcg ttgtgtgcag acgtgacgat atgcatcgta agcaacggga ggaggccaag
     5701 aatgcctatc gcaatcgaat agccgaagag attgtcagtc acttcgaacg cataccacag
     5761 cgcgccacca agcgccgcga tacggagtgt ccacgtcgtg cgaatcacca ggagcgagaa
     5821 tgcgcccaca gcaactgcta acgcgtacgg gcggcttgcg ccgccaaagt catccgtata
     5881 gcaccaccgg caccattcgg attgcagaag aaaaacggaa gtgagaaaga tcagtctata
     5941 aagaatgcta ctcatggtgt caccatcctt tctaaaggtt gtgacgtcac ttcaaggctc
     6001 gggagaccca atgtctccac cgaaagccgg gaaatgacaa aacctgccga gtcagcaggg
     6061 atctgtatgg ttataacaca ctacttaact cttacaaaat taccggttct tgccgtggaa
     6121 tttcttatgc acctcgcgta aatgcgcatc agtaacgtgg gtatatatct gtgtggtgtt
     6181 tatcgaggcg tggcccagaa gcgcctgcac ggagcgaatg tctgctccat tacttaaaag
     6241 atccgtagca aacgagtgac gcaatacgtg gggcgtcact ttcttggtga ttcctgcctt
     6301 ggcggcatac gtctttatca tcatttgtac tgcgcgtggg gtgaggcgcg ccggcttctt
     6361 cttactcgtc ttatcaacgg ctgacacgtt cacgaatagc gcttcttcca tatctttgcg
     6421 cttcttcagg tagtcgcgta ccgcttcctt tgcagaaggg gagatgaaca ctacgcgtac
     6481 cttgtctccc ttcccgcgta ctgaaagttc atcgcgagag agatcaatat cggaattaag
     6541 accgcaaagc tcagagacgc ggagtcccgt agagaaaagc agctcgagaa tggcgcgatc
     6601 acgaagcgcc ttctcgttag cgcctttggc agatccgata agtcgctcga gctcgacgtt
     6661 ggttatgaga tccaacgagc gctcgggaag cttcgcaagc tcaatgcgtt caggagagat
     6721 gcagggaatc tcacgctttc gcagaaactt aagaaacgca cggagcgcga tgaggtagta
     6781 gttctgcgtg cgtcgc
//
LOCUS       DAORIN010000025         6588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2148_length_6588_cov_5.672126, whole genome shotgun sequence.
ACCESSION   DAORIN010000025 DAORIN010000000
VERSION     DAORIN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_6588_cov_5.672126"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2360)
                     /locus_tag="PK109_01610"
     rRNA            complement(<1..2360)
                     /locus_tag="PK109_01610"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(2748..2972)
                     /locus_tag="PK109_01615"
     CDS             complement(2748..2972)
                     /locus_tag="PK109_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24270.1"
                     /translation="MAHSDNPDFNENDYDTIDEWAKTILDEGGTIDYVFESDSQPVSI
                     DDAQHAIVDADGNKWLVRKVIIHLQPAAVK"
     gene            complement(3101..3781)
                     /locus_tag="PK109_01620"
     CDS             complement(3101..3781)
                     /locus_tag="PK109_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24271.1"
                     /translation="MFFSTWADVLTQSFQNLFYGLVAFLPNFVAALVIFIIGWLIGAG
                     LGRVVAQVINSLRVDQALKAAGVDRVVERAGFTLNSGAFLGVLVKWFFVIVFLVAALD
                     VLGLTEVTIFLRAVVLGYLPQVIVAVLILLVAAVVAEAAQRVVAGSAAAANLRSANFL
                     GAMARWAIWVFAILAALDQLGVTPFVQTLFTGVVVALALAFGLSFGLGGQAAAARYID
                     KLREQIKD"
     gene            complement(3923..4492)
                     /locus_tag="PK109_01625"
     CDS             complement(3923..4492)
                     /locus_tag="PK109_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24272.1"
                     /translation="MDPLAKLFGSAARVKLLRLFLFNENEFYAAADAASRAKVSKEST
                     RREVNALAAIGVLRKKTGKKGSMYAANPKFAHYEPLRTFLRTSSGVDDASITSTLKKA
                     GTLRLVALSGLFTGVPESKIDILIVGDRVDERTLGILVRGIEAELGRELRYASFTTED
                     FRYRVGVYDRLVRDVLDYPHRTILDKIGM"
     gene            complement(4576..4758)
                     /locus_tag="PK109_01630"
     CDS             complement(4576..4758)
                     /locus_tag="PK109_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24273.1"
                     /translation="MKKTLFTIVAVAFLFTILFQFLPTEWMDGVGIIIVGTLILSPVA
                     LLIVWVIEFFRKKKGT"
     gene            complement(4960..5307)
                     /gene="rplL"
                     /locus_tag="PK109_01635"
     CDS             complement(4960..5307)
                     /gene="rplL"
                     /locus_tag="PK109_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRH24274.1"
                     /translation="MVEEVEAMTVLELNELVKVFEEKFGVSATAVAAAGPAAGGAAEE
                     GKSTFDAELTSGGDQKIAVIKVVKEALALGLKEAKDLVDGAPTMLKAGLKKEEAEELK
                     KKVEDAGGKVTLK"
     gene            complement(5456..5944)
                     /gene="rplJ"
                     /locus_tag="PK109_01640"
     CDS             complement(5456..5944)
                     /gene="rplJ"
                     /locus_tag="PK109_01640"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRH24275.1"
                     /translation="MAITKAKKQDIVAKVADSLKNAVSVVFVRFNKISVADTSAMRKS
                     LKNEGVGYYVAKKTLLKRALKDAGYAGELPDLPGEVAIAWATTDATAAARGIHEHGKK
                     HEGLSILGGVFEGSYLDASGMKVIATIPSVPVLRGMFVNVINSPIQRMAIALGQIAAK
                     KA"
     misc_feature    complement(5968..6100)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(6100..6261)
                     /locus_tag="PK109_01645"
     CDS             complement(6100..6261)
                     /locus_tag="PK109_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24276.1"
                     /translation="MNERPHGDVMHYLMEFMKFLVGFSLMIAVSLTLLYFTTSSTDAA
                     LVAKPAVFA"
     gene            complement(6407..>6588)
                     /locus_tag="PK109_01650"
     CDS             complement(6407..>6588)
                     /locus_tag="PK109_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24277.1"
                     /translation="TEEFTFKVTFTPGEGGYDYRMQLLTIKFDTNNDTNGADNTTFTA
                     SPASDYETDFEFIAD"
BASE COUNT         1507 a         1823 c         1626 g         1632 t
ORIGIN      
        1 taaagctcct ggggtctttt cgtctaacta caggtaaccg gcatcttcac cggtagtgca
       61 ttttcaccga gcgagtcctc gagacagttc cccagtcatt acaccattcg tgcacgtctg
      121 aacttaccag acaaggaatt acgctcttcg attccttaaa gcacgcgaag gaccatatct
      181 tcatccacga attgcttcgt ggcgaatgac gtatggcctc ttggagtcat tgttggacaa
      241 gatacagatt gctccgtatc aaggtaactt ggcgtattct gttcacccta caatcgtccc
      301 agtccgttcc gggttccaat aaattgaaac ccggaacctt aggacgatta tagttatcgc
      361 cgacattcac cggggcttat acgatccagc cataaatccg aagactcatg acctttcgtg
      421 cttgaccttc cggcattggt caggtgtcac cccctataca tcctctttcg agttcgcagg
      481 gagctgtgtt tttggtaaac agttgccagg gagtctttcg ctgcggctct cattgctgag
      541 agcaggcctt atcgctaact tacggccgct attttgccga gttccttgag gacctctcac
      601 tcgttcgtct tggtcttctc gacctgatca cctgtgtcgg tttgcggtac ggtcatcatg
      661 tactgaagct tagaagattt tcttggaacc gcgctcactg atctctctcc ctccgaagaa
      721 ggaaattgtt gctccgcttg gagtatgtgc atcccggatt ttcctaggac acctcctcac
      781 ggcactaacc cacatccaat tgtgggcacc aattacagca atccgtctct ccatcgcata
      841 catgaatggt cagggaatat taaccctgtg tccatcgggt gcggttttca ccatcccctt
      901 aggcccgact aacccgaagt tgatcaccat agcttcggaa accttgatct ttcgacgtgc
      961 ggggttctca cccgcattgt ggttacttgt acctgcattc tcacttccgt acgctccacc
     1021 ctcggtcacc cgttaggctt catcgcagaa cggaacgctc tcctaccgct tggattgtcc
     1081 gaagacgcac caagcccgca gtttcggtat tacgcttaac cccgatcatc ttcggcgcaa
     1141 gaaccctcga tgagtgagct gttacgcttt ctttaaatga tggctgcttc taagccaaca
     1201 tcctcattgt caaaggatcc tcacttcctt aagttgtact cagcgtaaat ttagggacct
     1261 taactggcga tctaggctct tcccttctcg accaatggag cttcgccccc atggtctaac
     1321 tgccagactc tgacttccgg tattcggagg ttgataagag taacgagatt gctccctgtt
     1381 taccctttcc agtgctctac ccccggaagg aacatctgac gccagcccaa aagctgtttc
     1441 ggagagaacc agctattacg ttgttcgatt agcttttcac tccttaccac aagtcatccc
     1501 aagacgttga acgatctacg ggttcgggcc tccatctgtc tttcgacaaa cttcaccctg
     1561 ctcatggcaa gctcacaacg cttcgggtcc tatatatgac acaggcgcgc tattcacact
     1621 cggtttccct atggctccgc cccgtaacgg gcttagccga agcgacatat atagactcgc
     1681 aggctcattc ttcaataggc acgctatgaa ggacgcaagc gccccccata accctttgta
     1741 agtgcacggt ttcagttcta tttcactccc ctctcggggt gcttttcacc tttccctcac
     1801 ggtactagtt cactatcggt cttgcgacat atttagcctt accggttagt tccggcagat
     1861 tcacccgggc tattcgtgtc ccgagctact caagagcgga agcacataag tcatcatata
     1921 tttcgcgtac ggggccatta ccctctgggg cgctgcttcc cagcagcttc cgctatatag
     1981 atgttttgta actcatgcca tacaagtatg gcgcttccgt cttacaaccc cgacatccga
     2041 agacatcggt ttgggctcct cccgtttcgc tcgccgctac taagggaatc gcgagagtag
     2101 ttttccatac gcgcgaacat acgcacgcac ggaaaactac tcgcgtttgt tttcttttcc
     2161 tccaggtact gagatgtttc acttccctgg gttagctctc atattgctat gagtaatcct
     2221 atatggattg agtttcctca ttcggagacc ttcggatcga tggctaaaaa tcgcctcccc
     2281 gaagcttttc gcagattaat cgcgtccttc atcgctgtcg caagcctagg catccaccgt
     2341 gcacccttca tgtttcctgt gcggaatcat gaacaccaca atactttctt aactgcactt
     2401 ctcaccacgt cttggtttaa agacgtggct tgataccttt tgcgtattcg tgtgtcaaag
     2461 gcccagtgtc ccgaaaagcg aaaccgcccc ttccggggcg gtttccgacg cttacgcgcc
     2521 aggcggcctt acggcctttc cgctccagat aggatgagat gtcgcttagt agacacgtgt
     2581 aaagacgagt ctacgcgcca tgaaaaggac tgtcaagcaa aggagcggag gcggcgtacc
     2641 acggcctatt ccctatcccg ccctttgcct atccccacga aatccccata aaaaagagcc
     2701 acggcaggat gagtgccgcg gcccctattt tcgatctcgg tgaagggtta cttcaccgcc
     2761 gccggttgaa gatggatgat gaccttgcga acgagccact tgttgccgtc agcatcgacg
     2821 atcgcatgtt gcgcgtcgtc gatactgacc ggttgcgagt cagactcgaa gacgtagtcg
     2881 atggtgccgc cttcgtccag aatggttttc gcccattcat cgatggtgtc gtagtcgttt
     2941 tcgttgaagt ccgggttatc actatgcgcc ataatcttag ttcctcatgg acgcgctaga
     3001 ttccgtctag ctccgggtgc agtgaaagta ttatactaca tttcatgtta aaagcaaaag
     3061 gcgcccgaag gcgccttttg caaaaccatt ggagagcgaa ctagtccttg atctgctcgc
     3121 ggagcttgtc gatgtagcga gctgccgctg cctggccccc aaggccaaac gagagaccga
     3181 acgcaagcgc aagcgcaacc acgacgcccg tgaagagagt ctgcacgaac ggcgtgacgc
     3241 caagctgatc gagggcagcg agaatcgcga atacccagat agcccaacgt gccatagcgc
     3301 cgaggaagtt tgctgagcgg aggtttgccg ctgcagcgga tcctgcgacc acgcgctgtg
     3361 ctgcttctgc caccactgcg gcgacgagaa ggataagcac ggcgacgatg acctgaggga
     3421 ggtagccaag tactaccgcg cggaggaaga tcgttacttc cgtaaggccg agcacatcaa
     3481 gtgcggcgac caagaagacg ataacgaaga accactttac gagaacgcca aggaacgcac
     3541 ccgagttaag ggtgaagcct gcgcgttcaa cgacgcggtc aacaccagct gccttaaggg
     3601 cctggtcgac gcgaagtgag ttgataacct gtgccaccac gcgcccgagt ccagctccga
     3661 tgagccagcc gatgatgaag ataaccaatg ctgctacgaa gttcgggaga aacgccacga
     3721 gtccgtagaa gaggttctgg aaggactggg tgagcacgtc agcccaagta gagaaaaaca
     3781 taatgcttgt agtgaatgga ttaattaagt aatacgggcg caggtcaagg tcctgctccc
     3841 taaagtgtag caagcttaga ccccccttcc ctggggcatg tggattatga aggacggccc
     3901 cctccggggc cgttttcctc tcttacatcc ctatcttgtc caaaatagtg cggtgcgggt
     3961 agtccaacac gtcgcgcacc aggcgatcat atacgccaac acggtaccgg aagtcttcgg
     4021 tggtgaatga ggcgtaccga agctcgcgtc caagctccgc ttcaatgccg cgcacgagta
     4081 tcccgagcgt tcgctcgtcg acacggtcac ccacaatgag aatatctatc ttcgattcgg
     4141 gcacaccagt gaaaagtcct gagagcgcga ctaggcgaag cgtacctgct ttcttaagcg
     4201 tagacgtgat gctcgcatca tcaaccccag aagacgtacg taggaaagtg cgtagtggct
     4261 catagtgcgc gaacttagga ttcgccgcat acatgcttcc cttcttcccg gtcttcttgc
     4321 gcaggacacc aatagcagca agtgcgttca cttcgcgacg ggttgattct ttgcttacct
     4381 tcgcacgcga tgcagcgtct gctgctgcgt agaattcgtt ctcattgaag aggaataagc
     4441 gcaacagctt cacgcgggca gccgaaccga aaagctttgc gagtgggtcc atagtggggc
     4501 ttattgtacg gacccttgac ggcattatgc aagtgcgcga aaaaccccgt tttcagggcc
     4561 tttttgctag ggaatctatg ttcctttctt ctttctaaag aattcgataa cccagacgat
     4621 gaggagtgcg actggggaaa gaatcagggt ccctacaatg atgattccta caccatccat
     4681 ccactcggtc ggtaaaaact gaaaaaggat ggtaaagagg aacgccacag caacgatagt
     4741 gaaaagggtc ttcttcatag aagaaagtgt agcatcgctc aaatcaagct agttaaaaga
     4801 aaaagccccg tttccggggc tttttctttt tctaaacttt tccgcgatag cggaaagtaa
     4861 ctttgaatag cgtagcgatt gacttgccag cgagtgagcg aaatgaatat ctgatattca
     4921 tttccgaacg agcgcctgcc agcaagccgt tcctacggct tatttcaaag ttacttttcc
     4981 gcctgcgtcc tcaaccttct tcttaagctc ttcagcttcc tccttcttga ggcccgcctt
     5041 gagcatggtt ggagcgccgt caacgagatc ctttgcttcc ttgagaccaa gggcaagggc
     5101 ttccttaacg accttgatga ctgcgatctt ctgatcgccg cctgaggtga gctcagcatc
     5161 gaaggtagac ttaccttctt cagctgcgcc accggctgca ggacctgcag cggcaactgc
     5221 cgtagcagat acgccgaact tctcttcgaa gaccttcacg agctcgttga gctcaagaac
     5281 ggtcatcgct tcaacttcct caacgatctt cttgaatttc gctgggactt ctactttctt
     5341 ttcttctgct gctggagctg caggcgctgc aggcgctgcc gtcgtttcct cgggagctgg
     5401 cgctgctgcg tcctggttca tttcatctgc catataagta aatgagtttt ataagttatg
     5461 ccttcttcgc ggcaatctgc ccaagggcga tagccatgcg ctggataggg ctgttgatga
     5521 cgttgacgaa catgccacgg agcaccggaa ctgacggaat agtcgcgatg accttcatac
     5581 ccgaagcgtc gaggtagctg ccctcaaata cgccgccgag aatagaaaga ccttcgtgct
     5641 tcttaccgtg ttcgtgaata ccacgtgctg ctgccgttgc gtcagtcgtt gcccacgcga
     5701 tagcaacctc acccggaaga tccggaagtt cacctgcgta gccagcatcc ttcaacgcgc
     5761 gcttcaagag cgtcttcttt gcaacgtagt agccgacgcc ttcgttcttc aacgacttac
     5821 gcatcgctga ggtatcagcg actgaaatct tgttgaagcg aacgaatacg acggaaaccg
     5881 cgttcttaag agaatccgcg actttagcga cgatgtcctg cttctttgct ttggtaattg
     5941 ccatgtatgt ttggttcgtt cttttgtacg gaaacgaaca gagtcccttt cgggactcgt
     6001 ggagacagtc gaccaagagc agtaatgctc tcgtcgtccc tcggcaggaa attaaggctc
     6061 gaaagccacc tgctgtcttc ggtccgtaca agagggactt tacgcgaaaa ccgccggttt
     6121 tgcaacgagg gcggcgtcag tgcttgaagt cgtgaagtaa agaagggtaa gagagaccgc
     6181 gatcataagg gagaagccca cgaggaattt catgaattcc atcaaatagt gcatcacgtc
     6241 tccgtggggg cgctcattca tacttccaaa acgatagcac aaagccccag ccgtgaggcc
     6301 ggggctttgt ggaaaccaaa aaccgccccg taggggcggt ttttggcaat tctgattcgc
     6361 ttctttcgaa gctaagacaa gtttgggaca gtgcccgaac tagtgcttag tcagcgatga
     6421 actcgaagtc agtctcgtag tcgcttgcag gtgacgcagt gaaggtcgtg ttatctgcac
     6481 cattcgtgtc gttgttcgtg tcgaacttga tggtgagaag ctgcatgcgg taatcgtaac
     6541 caccctcgcc tggagtgaag gtcaccttga aggtgaactc ctccgtcg
//
LOCUS       DAORIN010000026         6490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2202_length_6490_cov_5.207148, whole genome shotgun sequence.
ACCESSION   DAORIN010000026 DAORIN010000000
VERSION     DAORIN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2202_length_6490_cov_5.207148"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..1836)
                     /gene="rny"
                     /locus_tag="PK109_01655"
     CDS             complement(307..1836)
                     /gene="rny"
                     /locus_tag="PK109_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRH24278.1"
                     /translation="MSIKLVLSLLAFSGLAGIALGYVLRLLIALGQRGSLELEVKQKL
                     LEARERAVQIEKEAEMRADQIEAEKLGPIEEREERIEKTSERLEKREEFLDERERTLA
                     EEQERVKSRENEAANLKRDAENVLKERRAELVRIGHLSEKEARERLMKEVEAVEEESV
                     LTRIQKLEANGRERLEEKARSILMSVIHRMGNAVNAEVMTLSIQIPSEEIKGKVIGKE
                     GRNIKAFEKATGVDVIIDDAPDKITLSSFDPLRRHIAKIALERLIADGRIQPAKIEEL
                     VEKAKQEVEELVKQQGEKAAFEVGVVGLDPRLTTLLGRLHFRTSFGQNVLQHSVEMAH
                     IAGMLAEELGADVVVAKTGALLHDIGKAVDHEVQGTHVEIGRRLLEKFAIDTRVIQAM
                     QSHHEEYPYETPESIIVQVADAVSAGRPGARRDTVERYLERLGDLERIAKGFEGVEKV
                     YAIQAGREIRVFVNPGKVSDLAMHKLAREIAKRVQEELKYPGEIKVNIIRENRVVEFA
                     R"
     gene            complement(2585..3190)
                     /gene="clpP"
                     /locus_tag="PK109_01660"
     CDS             complement(2585..3190)
                     /gene="clpP"
                     /locus_tag="PK109_01660"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRH24279.1"
                     /translation="MATNSPRSSMLVPMVIEKSQFGERAYDIYSRLLKERIIFLSGGI
                     EDDMANLIVAQLLFLEAEDPKKDISLYINSPGGSVTAGLAILDAMNHVKPAVSTVVVG
                     IAASAAAVILAAGEKGKRYALPNAEVMIHQPWGGAQGQATDIEITAKHILATRDRLNK
                     ILSKATGQPLAKIEKDVDRDYFMMADDAKKYGLVDTVYKSK"
     gene            complement(3233..3412)
                     /locus_tag="PK109_01665"
     CDS             complement(3233..3412)
                     /locus_tag="PK109_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24280.1"
                     /translation="MDESAVSIPARPKSTLGSLVTAGIIGALIAITLLYMWGARIAEE
                     KGTEVLQPSTERALP"
     gene            complement(3422..3607)
                     /locus_tag="PK109_01670"
     CDS             complement(3422..3607)
                     /locus_tag="PK109_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24281.1"
                     /translation="MEPETSTTAAAPVDAPKKKSPGALIGLLIILLAIVAAAFYMLTE
                     RTHTVPNAAAVEETSTL"
     gene            complement(3633..4184)
                     /locus_tag="PK109_01675"
     CDS             complement(3633..4184)
                     /locus_tag="PK109_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRH24282.1"
                     /translation="METIKYQGKILEIVEFDVSQAGKTMVFEKARRSPGVRLIIPHEG
                     TILLSKEDRHEVGGYDYRLPGGKVIDKLADYNATLASGVDMKEVAKEAAVREAREEMG
                     LTVDATDLTFFHKSVCGTTVEWDLYYFVVNSFGKTEQEFDEGEDITLSPFDRETVKKM
                     CLDGSINEERSALVLLKYLAQNA"
     gene            complement(4253..4783)
                     /locus_tag="PK109_01680"
     CDS             complement(4253..4783)
                     /locus_tag="PK109_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRH24283.1"
                     /translation="MADYDKLYPPTDIESGVITTVIEVSKGSILKIEYNREKQTFELD
                     RVEPEIFAKPANYGFIPGTTDEDGDALDTLVVTPEPMPTGIALKARVLGVLNFEDGGE
                     KDHKIICVPVDDRDGGDRVKTLEDLGEQWKAKVEYHFNTYKDLKKRGTTKTLGYGGPE
                     DALHVITECIARYAGK"
     gene            complement(4813..6162)
                     /gene="nusA"
                     /locus_tag="PK109_01685"
     CDS             complement(4813..6162)
                     /gene="nusA"
                     /locus_tag="PK109_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRH24284.1"
                     /translation="MLDLKTIQSVLGEFEERGITKETMIDAIESAMATAYKKEYGKRG
                     QVVRAVLDMNTGTVAFEQVKTVVDDTMVRFPEEGEAEPEYHPHRDYEETPEGELPRYD
                     AEKHILLEDARRIRRDAGLGSEIVFPLEPQDDFGRIAAQTAKQVVIQKIREAEKLSIL
                     DEFGHKKGEIVSGLVQRVERGSLFIDLGRATGVIPYEEQIPGERYRPGERIRAYLNAV
                     DEGFRGVYLRLSRAHPKFIVKLFEMEAPELAAGSIEVKNIAREPGSRTKIAIASLDPH
                     VDPVGSLVGQRGVRVSTVMSELGGERIDIIEWSEDPKNFIKESLSPATVMDVVLDEAE
                     HRAAVTVSEDQQSLAIGRGGQNVRLAAKLTGWNIDINSVEGEKPKEEETVVSVTPDDA
                     ATPSEEAGIAPTETPAEVVADDSIAAAELPAEEELTTSPEGEVSVVDSEEARDKENA"
BASE COUNT         1438 a         1755 c         1668 g         1629 t
ORIGIN      
        1 gaagatgccg agtcctgcta ccgagacttc gtcaccagcc ttgaggcctg ccatgacgga
       61 ctcgataaag gtctcgaccg cgcgctcagc gtctgccttc gtgccgccaa gggtggcctg
      121 cactttcgat acgatatctg ctttgttcat acttcgtaag ttaggttcgt aatcccgcgg
      181 ccgaggaggc agggggtaca aggaaagtat agaaagtacg ccgttttgcg caatagggct
      241 tgctttttca gggggatatt gaaaaatccg cctttcagcg gatttttctc atcatttctt
      301 cgacaattat cgtgcgaatt cgacgacgcg attctcgcga atgatgttta ccttgatttc
      361 tcccggatat ttcagctctt cctgcacgcg ctttgcgatt tcacgcgcaa gtttatgcat
      421 tgcaaggtca gaaacctttc cagggttcac aaatacgcgt atctcgcgtc ctgcttgtat
      481 cgcgtatacc ttctctacgc cctcaaatcc tttcgcaatg cgctccaaat cgcccagacg
      541 ttcaaggtag cgctcaacgg tgtcacggcg tgcacctggg cgtcctgcag ataccgcatc
      601 agcgacctgc acgatgatgg attccggtgt ttcatacgga tattcctcgt gatgcgactg
      661 cattgcctgt atcacgcgcg tatcaatcgc gaacttctca agaagacgtc gtccaatctc
      721 aacatgggtg ccctgtacct cgtggtccac tgcttttccg atgtcatgca gcagtgctcc
      781 ggtcttagca acgacaacgt cagcgcctaa ttcttcagcg agcatgccag cgatgtgggc
      841 catctccact gagtgctgga gtacattctg accaaagctt gtgcggaagt ggaggcgacc
      901 aagaagggta gtgaggcgcg gatcaaggcc cacgacgcct acttcaaagg ctgctttctc
      961 cccctgctgc ttcacgagtt cctctacctc ttgttttgcc ttctcgacga gctcctctat
     1021 cttcgccggc tgaatgcggc catcagcaat gaggcgctca agcgcaatct tagcgatatg
     1081 acgtcggagc gggtcaaagg acgagagcgt tatcttatcc ggagcatcat cgatgatgac
     1141 gtcgacgccc gtcgccttct caaacgcctt gatattacgg ccttccttac caatgacctt
     1201 acctttaatc tcttcagaag gaatctgaat ggagagggtc attacctcag cgttcacggc
     1261 gtttcccatg cggtgaatga cggacatgag gatggagcgt gccttttctt caagacgctc
     1321 acgaccattg gcttcaagct tctggatgcg ggtaagcaca gactcttctt ctaccgcctc
     1381 cacttctttc attaaacgtt cgcgcgcctc tttctcagaa aggtgtccga tgcgtacgag
     1441 ttctgcacga cgttccttaa gtacgttttc agcatcgcgc ttcaggttcg ccgcctcgtt
     1501 ctcacggctt ttcactcgtt cctgctcttc ggcaagggta cgctcgcgtt catctaagaa
     1561 ctcctcacgc ttttcaagac gctccgaggt cttctcaatg cgttcctcgc gctcttcgat
     1621 agggccgagc ttctctgcct ctatctgatc agcgcgcatt tctgcctctt tctcaatttg
     1681 cacagcgcgt tcacgtgcct caagcagctt ttgcttcact tcaagctcca atgacccacg
     1741 ctgcccgagc gcgatcagga gacggagcac atacccgagc gcaatgccgg caaggccgga
     1801 gaacgccaaa agacttaata ctagttttat agacatacga attcagaact tacgcacttt
     1861 ttagtcgcac aaaaaatcta caagatgtca gcggaatctc agtcgtttct gccacagtcg
     1921 ttgctgacgc taggactcta tcacgcttcc ttgacttgcg cgaagccttg tagcatggtg
     1981 tcattctctc cgattgggac ataaggagca ctacgtgtac cactacatca gtgtggacgg
     2041 gttcgcaggg aaccttgagg ttcaaggaag caaggttgtc gctatcgcgc tagccatggc
     2101 gctctttggg tacgaaatct gcagtccacc gcaaacaccc aaacgacgga agaaaggatt
     2161 gctctggttt cgtaggccgg aaggcccgta ctggaatact ccagttggcg ggcagctcaa
     2221 actgctctga tcaacatacg gcgcggcccc tcacgggtgt ccgcgctgtt tttatgcaca
     2281 gcaaagaatg agcggatgcg gagtataact aacagtatcc agtaactctg cggagggaag
     2341 gacatggcta aagctctttg catccttgta gcgtgggtac tgctcgcatc attcgcccct
     2401 tctaaacaca tcgcgactca tacgacggtg cgtctcacga gatgggaagg aaatgctccc
     2461 atcgagcgga cagagcgaac gtgggatgag accacaccgg aatgcgcgcc tacaaacgga
     2521 tggcacgtat gcacctaaag aaaaggcgcg atccattgat cgcgccatct tcttgtaggg
     2581 agccctactt actcttgtag accgtatcca cgagcccgta cttcttcgcg tcatccgcca
     2641 tcatgaagta gtcgcgatcc acatccttct caatcttggc gagcggttgg ccggttgcct
     2701 ttgaaagtat cttgttcaaa cggtcacgcg tcgcaagaat atgctttgcc gtgatttcaa
     2761 tatcagttgc ctgaccttga gccccacccc atggctggtg aatcataact tcagcgttcg
     2821 gcaatgcgta gcgtttaccc ttctctccag cagcaagaat gacggcagca gcgcttgcag
     2881 cgatacccac tactacagtg gagaccgccg gcttcacgtg gttcatcgcg tcaagaattg
     2941 cgagtcctgc ggtgactgag ccgcccggag agttgatgta gagagaaata tctttcttcg
     3001 ggtcctcagc ttcaaggaaa aggagctgag cgacgataag gttcgccata tcgtcttcga
     3061 tgcctcctga gaggaagatg atgcgttcct tcaagagacg cgagtaaatg tcatacgcgc
     3121 gttcgccaaa ctgagatttc tcgataacca tcggtacgag catagatgag cggggtgaat
     3181 tggtagccat agtagggcta tcgtagcaga aaaagagagg cgcgccaaaa acctacggaa
     3241 gtgctcgttc agtagatggt tgcagtactt ctgttccttt ttcttctgcg atacgtgcgc
     3301 cccacatata aagaagcgta atagcgatga gcgctcctat gattcctgcg gtaacgagtg
     3361 agccaagcgt acttttcggg cgtgcaggaa ttgatacggc tgattcatcc atacgtggta
     3421 gctagagcgt cgacgtttct tcaacggcgg cagcatttgg aactgtgtgg gttcgctccg
     3481 tgagcatata gaatgcagcg gcaactatcg caaggagaat aatcagaaga ccaatgagcg
     3541 cgcctggaga tttcttcttt ggagcgtcga cgggagctgc agcggtggta ctagtttctg
     3601 gttccattat ctcctagtat atcgtctttc tcttatgcat tctgtgcgag gtatttaagg
     3661 agaacgagtg cgcttcgttc ttcgtttata gaaccatcga gacacatttt tttcaccgtc
     3721 tctctatcaa acggggagag cgtaatatct tcgccctcat cgaactcttg ctctgttttt
     3781 ccaaatgagt tcactacaaa gtagtagagg tcccattcaa cggtggtgcc acacacagat
     3841 ttatgaaaga aggtaagatc cgttgcgtcc accgtaagcc ccatctcttc gcgcgcttcg
     3901 cgaaccgctg cctctttcgc tacttctttc atatcaaccc ctgaagcaag agtcgcgtta
     3961 tagtcggcga gcttatcaat gaccttgccg cccggaaggc gatagtcgta cccgcctacc
     4021 tcgtggcgat cttccttgga gagaagaatc gtcccttcgt gtggaatgat aaggcgcact
     4081 ccaggggaac gacgcgcctt ctcaaagacc atggtttttc ctgcctgact tacatcaaac
     4141 tcaactatct ccaatatctt tccctggtat ttaatggttt ccattgtcgg cactatagca
     4201 gaacgggcga ccctttgaag ggccgcccgt tctggaataa atgtaagaac gcttactttc
     4261 ctgcgtaacg tgcgatgcac tccgtaatta catggagggc gtcttccggg ccaccatacc
     4321 ctaaggtttt ggtggtaccc cgtttcttca agtccttgta cgtgttgaag tggtattcga
     4381 ctttcgcctt ccactgctcg ccaaggtctt cgagcgtctt cactcgatcg ccgccgtcgc
     4441 ggtcatcgac cggaacgcag ataatcttat gatccttctc gccgccgtcc tcgaagttca
     4501 agacgccgag tacgcgtgcc ttaagcgcaa taccggtagg cataggctca ggagtaacta
     4561 cgagggtatc aagcgcgtca ccgtcttcgt cggtagttcc tggaatgaag ccgtagtttg
     4621 ctggctttgc aaagatttcc ggttccacac gatcaagttc gaaggtttgc ttctcgcgat
     4681 tgtattcaat ctttaggatt gaacctttag agacttcgat aacggtggta ataacgcccg
     4741 actcgatgtc tgtcggaggg tagagcttgt cgtaatcagc catagtaatg agtagttagc
     4801 tgttcgattc tactacgcgt tctccttgtc gcgcgcttct tcgctgtcga caaccgagac
     4861 ttcgccttca ggagaagtcg taagttcttc ttcggcagga agctcagcgg cagcgatgct
     4921 gtcgtcggca acgacttcag ctggagtttc cgttggggca atacccgctt cttctgaagg
     4981 agtagcagcg tcgtcaggag tgacggacac gacagtctcc tcttcctttg gcttctcgcc
     5041 ttcaacggaa ttaatgtcga tgttccagcc agtaagcttt gcggcaaggc gcacgttctg
     5101 gccgccgcga ccaatggcaa gcgactgctg gtcttcagat acggtgaccg cagcgcgatg
     5161 ctcggcctca tcaagtacta cgtccatgac ggtagctgga gaaagagact ccttgatgaa
     5221 gttctttggg tcctcgctcc attcaatgat gtcgatgcgt tcgccaccga gttcgctcat
     5281 aacggtagag acgcggacgc cgcgctgacc gaccaaagat cctacgggat caacgtgcgg
     5341 atcaagggaa gcgatagcaa tcttcgtgcg agagcctggc tcacgcgcga tgttcttcac
     5401 ttcgatagag cccgcagcaa gttccggagc ttccatttca aagagcttca cgatgaattt
     5461 aggatgtgcg cgggaaaggc gcagatacac accgcggaag ccttcgtcta ccgcgttaag
     5521 gtaggcacga atgcgctctc ctgggcggta acgctctcca ggaatctgct cttcgtaggg
     5581 aataacgccg gtagcgcgtc caaggtcgat gaagagggag ccgcgctcaa cgcgctgcac
     5641 caagcccgac acgatctctc ccttcttatg accgaactca tcgaggatag agagcttctc
     5701 tgcctcgcga atcttctgga taacgacctg ctttgcggtc tgggcggcaa tgcgaccaaa
     5761 gtcatcctgt ggttcaaggg gaaacactat ttcagaccca aggccggcat cacggcggat
     5821 gcggcgtgcg tcctcaagga ggatgtgctt ctccgcgtca tagcggggaa gttcgccttc
     5881 aggcgtttcc tcataatccc ggtgggggtg gtactcaggc tcagcttcgc cttcttcagg
     5941 gaagcggacc atggtgtcat cgacgacggt cttcacctgt tcgaaggcaa cggtaccggt
     6001 attcatatca agaacggcac gcacgacctg gccgcgcttt ccgtactcct tcttatacgc
     6061 agtggccatc gcgctctcaa tagcgtcgat catggtctcc ttcgtgatgc cgcgttcttc
     6121 gaactcgccg agcactgatt ggattgtttt gaggtctaac atatggaatg tgtatgtctg
     6181 gctaaaaaga aatgtctgca ggtgcagaca tatcaggtac ccggagagta ccaaaaatag
     6241 ggtgaggtgt caatgaatga tttgacatac taaattatgt gttgcacagt ataggtcccc
     6301 ataaacccct agaagcctga ggaggcgact ttgcggaatt acctggtact ggcggcgctc
     6361 tttcttacag cgtgcgataa cactgaggcg ctccaacgtg ctgaacgggc acgtgaggtc
     6421 gagtcccgat gcgagtgctt agagctcgaa gaaggtctcg tacgcgctcg gttcaactca
     6481 ccgatgcgtg
//
LOCUS       DAORIN010000027         6252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2335_length_6252_cov_5.480394, whole genome shotgun sequence.
ACCESSION   DAORIN010000027 DAORIN010000000
VERSION     DAORIN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2335_length_6252_cov_5.480394"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK109_01690"
     CDS             <1..181
                     /locus_tag="PK109_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24285.1"
                     /translation="EGHSEGEVKEVAALLGFDWKDAHFCSADKMYMERYGVTKEFVNI
                     EISRLTFDIPNPFQK"
     gene            209..291
                     /locus_tag="PK109_01695"
     tRNA            209..291
                     /locus_tag="PK109_01695"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Tyr,seq:gta)
     gene            299..2431
                     /locus_tag="PK109_01700"
     CDS             299..2431
                     /locus_tag="PK109_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRH24286.1"
                     /translation="MNTFRHFLALLLLLAFCVEPLAVLAQTVEEEPVITEEVAVTEPA
                     IPEETAYDASLPGEILATSTVEVIVEEAPVVEHIELAPGTYVPGEVIVKYEDEETAEV
                     VSTKGKGVAEVIADLASDEDIEYAEPNYVRGFEAIATDDTHRALLWGLENTGQAVNGT
                     TGTADVDTDAPEAWTLSTGEDVIVAVIDSGVDYTHPDLAANMWDGTNCVDETGAPLGG
                     CVHGYDFKDNDKMPLPPTGTDTLARHGTHVAGTIAAAMNNATGIIGIAPNAKIMAIRF
                     NLNTATEAKAIDFARENGARIINASFSGDGISAVEQEAIERFTDAGGIFIAAAGNGAA
                     DSIGDNLDNLPTPENASTTSAYPASYPLAGVVSVAATTQNDALASFSNYGATSVDLGA
                     PGVNILSTFRDNTYGYFSGTSMATPHVAGAAALLSAYRPDFTASDIKAALLDTGDAVA
                     ALSGKTVSGKRLNALAALQASPDTTAPVITLLGDTPLALSVGATFTDPGATAHDAVDG
                     DVTSSIVASGAVDTAIAGTYTRTYVVGDAAGNIATSTRDVTVSAVISSGGGGGGGGGG
                     GGSGSKNKKKKDTKTKTKTEVKKTEKPLPRLPQTATPQVLGTASYQFTRNLAQGATGA
                     DVTELQKALATLGFFSTTPTTYFGPMTATAVSAFQKAKGLPVTGAFDAGTRTALTGTT
                     PTSNDAQIAALTAQVAQLMTLLKQLQGK"
     gene            2461..3399
                     /gene="trpS"
                     /locus_tag="PK109_01705"
     CDS             2461..3399
                     /gene="trpS"
                     /locus_tag="PK109_01705"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRH24287.1"
                     /translation="MSKQRLLTGLQSSGDLHIGNYFGVLKPFRDTYENYESFLMVADY
                     HALTSLKDGGKLRENIQNAVKDYLAVGVDPKKAVIFKQSDVSAHTELAWIFNCLVTVK
                     FLELGHAFKDKVAKGLEANAGLLTYPMLMAADILLYDTDVVPVGEDQRQHLEYAREAA
                     AKFNNAYGALMKEPKELILPGVGVVPGTDGAKMSKSYGNTIPLFGTKDEVAKAIMGIV
                     TDSSGERPENVYAIHKLFKDEGTLTALYEEKKGKYKDLKEALIEDAEAELAPMRERRA
                     SITDAEVAAVLKDGAEKAKAVADAKMVAVRTAVGVA"
     gene            3508..4521
                     /locus_tag="PK109_01710"
     CDS             3508..4521
                     /locus_tag="PK109_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24288.1"
                     /translation="MEGYKAPEHKPEGIGRAINALEKALERHTLGKEGIKRLAASAGK
                     AVRYSLMGAMFLGLAGAGNHIRTRYEVTTTENGGEFAFTHPDEETTHLIEFLTGREDA
                     TEEFKLLSDRETLRREYAMRDYEIPENIEHMTREEIRAEVVQLFEIDGQVSAEAYADT
                     VLGAPYARIAFDPAMHRTVWETFAENGAPRVRLATAEDDSLTGRVIGNGRAHYDPLTN
                     TLYINPSNPRSHYLDELAHARQFDQDPAGSYALVVKSLVETLVDAVRHRESLQDAQHR
                     QYSRPGTLEHNAHGSRSSDPILTTDDLIQNGGMAGEIIDSMQARYHELNEERNGPQGG
                     NEE"
     gene            4583..4834
                     /locus_tag="PK109_01715"
     CDS             4583..4834
                     /locus_tag="PK109_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24289.1"
                     /translation="MPLNNETVIPVTTLTLIEGTETSWSKKMLDRIFTNGWVFVSKDS
                     LEAQTALPVEITEDGAPWLVKIHYSEEDAFRVGVPAVTY"
     gene            4931..>6252
                     /locus_tag="PK109_01720"
     CDS             4931..>6252
                     /locus_tag="PK109_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRH24290.1"
                     /translation="MKDRIMIGDLATHVGETVSICGWVDVRRDQGKMVFFDFRDRSGY
                     VQGVVLPKSEAMEAAKELRNEYVVRVTGIVNARPEKNVQADKQNGSIELEITGIETLN
                     AAETLPFEITGDTSGIDESVRLANRHLDLRSPRMQRNMKIRSEFVRRCREFLFSKDFT
                     EIETPLLTESTPEGSRDFVVPSRLNPGKFYALPQSPQQYKQLLMTAGFERYFQTARAV
                     RDEDLRADRGFEHTQIDVEMSFVEMEDVMQNIEAMITKVVEDMGFTIKEKPFPRISYK
                     DAMEKYGADKFDLRTEEEKAQGILAYAWVVNFPFFEKTDDGKWTFTHNPFSMPIPEHV
                     DWLMKGENIENILTTQYDIVCNGYESGGGSIRAHKPEILEATYKVMGYTKEEMEDRVG
                     HMLDAFKYGTPPHGGIALGVERNIMNLTGETYLREVQTFPITHNKQTS"
BASE COUNT         1620 a         1607 c         1673 g         1352 t
ORIGIN      
        1 cgaaggacac tcggaaggag aggtaaaaga agtagcagcg cttctcggat ttgattggaa
       61 ggatgcgcac ttttgctctg ccgacaaaat gtacatggag cgctatggcg tcacgaaaga
      121 attcgtaaac attgaaatat cccgtcttac ctttgacata ccgaatccgt tccaaaaatg
      181 acgaaaatag gctagaatcg gcctagatgc gtcggtggta gagtggtcaa ttacaacagg
      241 ctgtaaacct gtcggcttcg gcctacggag gttcgaatcc tccccggcgc acccctatat
      301 gaatacgttt cggcatttcc ttgccttact tctcctcctt gcgttctgcg tggagccatt
      361 ggccgtactt gcccaaacgg tagaagaaga gcctgtaatc actgaggagg tagcggttac
      421 agaacctgct attcccgaag agactgcata cgacgcatca cttccgggag aaatactcgc
      481 cacttctact gtagaagtga ttgttgaaga ggcgcctgtc gtagagcaca ttgaacttgc
      541 tccgggaacg tatgtccccg gagaagtcat cgtgaagtat gaggacgaag aaactgctga
      601 agttgtttcg acaaaaggaa agggtgttgc ggaagtcatc gctgaccttg cgtccgacga
      661 agatattgaa tacgcggaac caaactacgt gcgcggattt gaggcgatag ccacggacga
      721 cactcatcgt gcgcttctct ggggacttga gaacaccgga caagcggtaa acggaaccac
      781 aggtacagca gacgttgata ctgatgctcc tgaggcatgg acactctcaa ctggtgagga
      841 cgtcattgtt gccgttattg atagcggcgt cgactacacg caccccgatc ttgccgccaa
      901 catgtgggat gggacgaact gtgttgacga gaccggcgca ccacttggtg gctgcgtgca
      961 cgggtacgat ttcaaagaca acgataagat gccgctacca cctacaggta ccgacacgct
     1021 cgcgcgccac ggtacgcacg tcgcaggcac tattgctgca gctatgaata acgcgacagg
     1081 gataattggt atagcaccaa acgcgaagat tatggcgata cgctttaatc tcaacacggc
     1141 aacagaagca aaagccatcg acttcgctcg cgagaacggt gcgcgcatca ttaacgcttc
     1201 tttttcagga gacggcattt cggcagtgga gcaagaagct attgaacgct tcacggacgc
     1261 aggcggcata ttcattgcag cagcagggaa cggggcggca gatagcatag gagataattt
     1321 ggataacctt ccaacgcccg agaacgcaag cactacgtca gcgtacccag caagttatcc
     1381 gctcgcaggc gtcgtgagcg tcgcggcaac cacccagaat gatgcgcttg cttccttctc
     1441 aaactacggg gcaacgtccg ttgatcttgg cgcgccgggc gtcaacattc tgagtacctt
     1501 ccgcgataat acgtacggct acttcagtgg tacctcaatg gccacgccgc acgtcgcggg
     1561 tgccgcagcc ttgctttccg cctatcgtcc tgactttact gcctcagata taaaagcagc
     1621 gcttcttgat actggggacg cggtagctgc gctttcagga aagacggttt ctggaaaacg
     1681 attgaacgca ctggcggccc ttcaagcgtc tccggacacg actgcaccag tgatcacgct
     1741 ccttggcgat actccactcg cgctttccgt aggtgctacg ttcactgatc cgggagctac
     1801 tgcccatgat gccgtagacg gcgatgtcac ttcttctatt gtggcgtcag gcgcggtaga
     1861 taccgctatc gccggcacct atacccgtac ctatgtcgtc ggtgacgctg cagggaacat
     1921 tgctacctca acacgtgatg tgacggtctc tgcagttatc tcttccggcg gtggaggtgg
     1981 tggcggaggc gggggaggcg gcagtggttc aaagaataag aaaaagaaag atacgaaaac
     2041 gaagactaaa actgaggtaa agaaaacgga aaagccgctt ccacgccttc cacaaaccgc
     2101 aacgcctcaa gtacttggta ccgcctccta ccaattcact cgcaatcttg cacaaggcgc
     2161 tacgggcgct gatgtcactg agttacagaa agcactcgcc acactcgggt tcttttctac
     2221 tactccaact acgtacttcg gtcccatgac cgcaactgct gtatctgctt tccagaaagc
     2281 gaagggtctt cccgtaacgg gagcgtttga tgcggggacg cgtacggccc ttaccggcac
     2341 aacgcctacg agtaacgacg cacagattgc cgcccttact gcccaggtag cgcagcttat
     2401 gacgctccta aagcagctcc aggggaaata gcgttcccga ttttctgcta tattggctgc
     2461 atgtcaaaac aacgcctcct taccggtctt cagtcttccg gtgaccttca tatcggaaac
     2521 tatttcggag tactcaagcc ctttcgtgat acctatgaga actatgagag cttccttatg
     2581 gtcgctgact atcatgcgct cacctcactc aaggatggcg gcaagcttcg tgagaacatt
     2641 caaaacgccg taaaagacta tcttgccgtt ggcgttgatc caaaaaaggc agtgatcttc
     2701 aaacagtctg atgtttctgc acacacggaa ttagcatgga tcttcaactg tctcgttacg
     2761 gtgaagttcc tcgaattagg gcatgccttt aaggacaagg tcgccaaagg gctcgaagcg
     2821 aacgcgggcc ttttgaccta ccccatgctc atggcggcag acattcttct gtatgacacg
     2881 gatgtggtgc cggtagggga agaccagcgc cagcatcttg aatatgcgcg tgaagctgca
     2941 gcaaagttta acaacgcgta tggcgctctc atgaaagaac cgaaagagct cattcttccg
     3001 ggcgttggcg ttgtgccagg cactgatggc gccaagatga gcaagagcta cggcaacact
     3061 attccgctct ttggtaccaa ggatgaggta gcgaaggcga ttatgggtat cgttactgac
     3121 tcttccggag agcgtccaga gaacgtttac gctatacaca agcttttcaa agacgaaggt
     3181 actctcactg cgctgtatga ggaaaagaaa gggaagtata aggacttgaa ggaggccctt
     3241 attgaagacg ctgaggccga gcttgccccg atgcgtgaac gacgcgcaag cattacggat
     3301 gctgaggtcg ctgcagtctt aaaggacggc gctgagaaag caaaagcggt agcagacgca
     3361 aagatggtag cagtacgtac agcggtaggg gttgcgtagt tatgaatgac gcgtcaatac
     3421 gtgcaatgag tctggcagag acgctctaca aagaagctga agcaactgct gctgaggaaa
     3481 ttgctgcact cgctgcagat ttaacgcatg gaaggatata aggctccaga gcataaaccg
     3541 gaaggaattg gtcgcgctat aaacgctctt gagaaagcgc ttgaaagaca tacgcttggg
     3601 aaagaaggta ttaaacggct tgctgcctca gccggaaagg cagtgcgcta cagtcttatg
     3661 ggcgctatgt ttctcggact tgcgggagca ggaaaccaca tacgaacccg gtatgaagta
     3721 accaccaccg aaaatggggg tgagtttgca tttacccatc cagatgaaga aaccacgcac
     3781 cttattgagt ttcttaccgg acgtgaggat gcgactgagg aattcaagct actttccgat
     3841 agggaaacgc tacgccggga atatgcgatg cgcgactacg agataccgga gaacatagaa
     3901 catatgactc gggaagagat tcgcgcagaa gtggttcaat tatttgaaat agacggacag
     3961 gtcagtgcag aagcgtatgc cgacaccgtt ctcggcgctc cctatgcgcg cattgctttc
     4021 gatcccgcta tgcatcggac cgtgtgggaa acgttcgccg agaatggcgc cccgcgtgta
     4081 cgcttggcga ccgcagagga cgacagtttg acgggacgtg tgattggcaa cggacgagcg
     4141 cactatgacc ccctgaccaa taccctgtac attaatcctt caaaccctcg aagtcattac
     4201 cttgatgagc tcgcccacgc ccggcagttt gatcaggacc cggcaggttc ctacgcactt
     4261 gtggtgaaga gcctcgtaga gacgctagtc gacgctgttc gacatcgtga atccctccag
     4321 gacgcccagc accgacagta cagtcgcccg gggacactcg agcataatgc acacggctca
     4381 cgctcaagcg atcctattct tactactgac gatttgatac agaacggggg gatggcagga
     4441 gaaataatcg atagcatgca ggctcgctat catgaactta atgaagagcg taacggtcca
     4501 cagggaggta acgaagaata gtgcactttt attgtgatgc tatcatttcg gcacgcagcg
     4561 tatggctgct atggagggcg atatgcctct caacaatgag acggtgattc cggtcactac
     4621 attaacgttg atagaaggta cggagaccag ttggtccaag aagatgctgg atcgcatatt
     4681 caccaatgga tgggtattcg tctcgaaaga ttcacttgaa gcacaaacag cgcttcccgt
     4741 ggaaatcaca gaagacggtg ctccctggtt ggtgaaaatc cactacagcg aagaggatgc
     4801 gttccgcgtc ggtgttccgg cagtgaccta ctagtctcaa ggcgcgtccg tctgcgatgt
     4861 atttcgcgga cgggcgtcct tctttaatga gttcgaaatt acgccataag cgtgctattt
     4921 tatcctctat atgaaggacc gaatcatgat aggagatctg gccacccacg tcggtgagac
     4981 cgtctccatt tgcggctggg tagacgtgcg tcgcgaccag gggaagatgg tcttcttcga
     5041 tttccgtgac cgctcaggct acgtccaagg cgtcgtgctt ccgaagagcg aggcaatgga
     5101 ggcagccaaa gaacttcgga acgaatacgt ggtacgcgtc accggtatcg tgaatgcgcg
     5161 tcctgagaag aacgtgcagg cggataaaca gaacggctcc attgagcttg agataaccgg
     5221 cattgaaacg ttaaacgccg ccgaaacgct gccgtttgaa ataacggggg atacgtcagg
     5281 catcgatgag agcgtccgtc ttgcgaaccg tcacctcgac ctacgttctc cacgcatgca
     5341 gcgcaacatg aagattcgta gcgaattcgt gcgccgctgc cgtgaattcc tttttagtaa
     5401 ggattttacc gaaattgaga caccactgct taccgaatct actcctgaag gctctcgcga
     5461 cttcgtcgtg ccatcacgcc ttaatcccgg aaagttctac gcacttccgc aatctccgca
     5521 acagtacaag cagctgttga tgactgccgg attcgagcgc tacttccaga ccgcacgcgc
     5581 tgtacgtgat gaggaccttc gcgctgaccg cggattcgag cacacccaga tagacgttga
     5641 gatgtcattt gtagagatgg aagatgtcat gcagaacatc gaggccatga tcacgaaagt
     5701 ggtagaggac atgggcttca ccatcaaaga gaaaccattc cctcgtattt catacaagga
     5761 tgcgatggag aagtatggtg cagacaagtt tgacctacgc actgaggaag agaaggcgca
     5821 gggcattctt gcctacgcct gggtagtaaa cttcccattc ttcgagaaga cggacgacgg
     5881 gaaatggacc ttcacccaca acccattctc tatgcctatt ccagagcacg tagattggct
     5941 catgaaggga gagaacatcg aaaatatcct taccacgcag tacgacatcg tctgtaacgg
     6001 atatgaatca ggaggcggct ccatccgtgc ccataagccg gagattcttg aagctacgta
     6061 caaagtcatg gggtacacca aggaagaaat ggaagaccga gtcggccaca tgctcgacgc
     6121 gtttaagtac ggcactcctc cacacggcgg tatcgcgctt ggcgttgaac gcaacatcat
     6181 gaacctcact ggtgagacct acctacgcga ggtacaaacc ttcccaataa cacataacaa
     6241 acaaacctca at
//
LOCUS       DAORIN010000028         6117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2417_length_6117_cov_4.618773, whole genome shotgun sequence.
ACCESSION   DAORIN010000028 DAORIN010000000
VERSION     DAORIN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2417_length_6117_cov_4.618773"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /gene="purB"
                     /locus_tag="PK109_01725"
     CDS             complement(<1..1258)
                     /gene="purB"
                     /locus_tag="PK109_01725"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRH24291.1"
                     /translation="MATNELTAISPLDGRYRSKVERLAPYMSEYGLIRYRVGVECAYL
                     MALARERKLPFTLSKAEEDILARIAAVSEADAALVKKIETEGVEGIPATRHDVKAVEY
                     YIKQRIRGTTLEEKAEWVHFALTSEDVNNIAYALMLSDALESVLVPRIVAVQEGLLTL
                     ARTYAATPMLARTHGQSASPTTFGKEMKVFSERIATALTSLKNIKIQAKLNGASGNWN
                     AHAIAYPEVDWMAFTSKFIDSLNVERTYKLEANLYTTQIECHDSYVAVFDTVRRINTI
                     LIDFSQDMWRYISDSWVTQKPKAGEIGSSAMPHKVNPIDFENAEGNFGMANALFEHFG
                     RKLPISRLQRDLSDSTTERNFGVSFAHSLIGYESLLTGLSRIEPNTGAMAEALAAHPE
                     VLAEAVQTVLRREGQIGAYEKLKDLTR"
     gene            complement(1261..2142)
                     /locus_tag="PK109_01730"
     CDS             complement(1261..2142)
                     /locus_tag="PK109_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRH24292.1"
                     /translation="MPASPRGPLIAEGKTKRIYEAPGEPTRAIVEYKNDITAFDNPEF
                     TKQFNKKAEYSNIVNARVFEMLNAAGIPTAFVRQLSPTEFLSEKCTMVPLEVVARRYA
                     VGSYLNRHPEFKQEGDVPHRFEEPVIEYFLKTTKGSLKSAAGETLVEGLDPLKGEEDP
                     FIPNPEDATWNLALSKKPSTDPAASLGRTIDSSKVLPSTDAIAEMTAMIRNTFLTLEA
                     FFAEHDFKFVDFKIEFGVTTDGRLVIADVIDNDSWRLRDKDWKDVSKQSFRNGEDMAT
                     IEDKYERVAMLMEKSKK"
     gene            complement(2162..3298)
                     /locus_tag="PK109_01735"
     CDS             complement(2162..3298)
                     /locus_tag="PK109_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRH24293.1"
                     /translation="MDDKKPNAYALAGVDVDIEAQASKIMYEASKETFKNRDGLIGQI
                     VAPFDDFAGIKMVSAANLPEGTFFSVGFDTAGTKVEIAERVGIHDTIAHDLFAMVCDD
                     AVLRGGEPVLVGSNLDIKTLGTDDCYLPIVRELAKGYVAAANEANVAVINGEIAQMGS
                     LMTGFGDFPYHWGAACVWFARKEKLFTGKEIKAGDSVVMLREHGFRANGWSLIRKIFK
                     EEYGENWHEAAFGESTLGIAALTPSVIYSRCVVALHGGFETEGSCTIHGVAHITGGGV
                     PEKMMRVLRPSGLGAKLTDLFDPPEVMAHCQKLGKISDHDAYRAWNMGQGMALITPEP
                     EKVVKEAQAFGIEAKIAGKITKEPGVTLVSRGTEKPGQTLEFDI"
     gene            complement(3300..3815)
                     /gene="purE"
                     /locus_tag="PK109_01740"
     CDS             complement(3300..3815)
                     /gene="purE"
                     /locus_tag="PK109_01740"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRH24294.1"
                     /translation="MNAAVGIIMGSDSDLPVMQEAAKLLDDLGIMYELTVVSAHRTPE
                     RLVEYATNAERRGIKVIIAGAGGAAHLPGMTASMTPLPVIGVPITTKAVKGLDSLLSI
                     AQMPPGIPVATVAVNGAKNAGILAASILGTGDTEIMDLVRAYKEKIKDEVLAKAQKLE
                     TMGYEEYLKEN"
     gene            complement(3818..4933)
                     /locus_tag="PK109_01745"
     CDS             complement(3818..4933)
                     /locus_tag="PK109_01745"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004043967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRH24295.1"
                     /translation="MKTLGIVGGGQLALMMAQAAKTLGIECVVLDPTPECPASAVARQ
                     VVGDFHDHATVLAFAKEAEVLTFEIESANADALAELAAQGLPVHPMPETLALIKDKLA
                     QKNFLSERGIPVAPYQDAPDEEAARAAGEVLGYPFLLKARSGGYDGRGNATINTPADI
                     APAFAKLGTNLYAEGFVSFEKELAVVAVRTVAGDIRTYPVVETVHERHICLEVLAPAP
                     VKESVRKKAETLAHYVLESFSGAGVFAIEMFMVDGDVLVNEIAPRVHNSGHLTIEGCA
                     TSQFENHVRAVMGMELGSTELIAPAAVMLNILGERTGPATPVGVEDAKALGGVAIHIY
                     GKKETKPDRKMGHITAVANSLLEARAKAEDAWRRVSI"
     gene            complement(4930..5700)
                     /locus_tag="PK109_01750"
     CDS             complement(4930..5700)
                     /locus_tag="PK109_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRH24296.1"
                     /translation="MATPRALIFSGYGLNCEEETKTGLTLAGATADIVHIEDVAKNPS
                     MLAKYQIAMFPGGFSYGDDTGAGKAYGARVKHHLGDELTKFLERDTLMAGICNGFQIL
                     TAAGILPGALITNESARYSCRWVDMEVVGDTPWLKGITKVSLPIGHGEGRYVDTEANL
                     DTLEGTGRVALKYVAGEMSEYQGLPYNPNGAMRDIAGVTGKEGRILGLMPHPDRATFF
                     EQRPDWTVLREQLEREGKKVPVYADGMAVFRNAVQYFS"
     gene            complement(5702..>6117)
                     /locus_tag="PK109_01755"
     CDS             complement(5702..>6117)
                     /locus_tag="PK109_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRH24297.1"
                     /translation="TFMSGRVPVTDAKENVSVYKAFSKASGKRLMSSALAVSRGGVAV
                     ALGKMALAGGLGATVSFTGDAHTALFSESTGRFLVTVAPQKEKAFLAAMKDLSVKKIG
                     TVEDEPTLRIKTGSVSTTLPLDALQEAYHIPYNDF"
BASE COUNT         1348 a         1674 c         1535 g         1560 t
ORIGIN      
        1 ctcgggtgag atctttgagt ttctcatatg caccaatctg cccttcacga cgaagtaccg
       61 tttgtaccgc ctcagcaagc acttcagggt gagcagcaag tgcttctgcc atcgcgcctg
      121 tgtttggttc tatgcgggaa agaccagtaa ggagcgactc gtatccaata agtgagtgtg
      181 caaaagaaac accgaagttg cgttcagtcg ttgagtcgga gaggtcgcgc tgcaggcgtg
      241 atatcgggag cttccgacca aagtgctcga acaacgcatt tgccataccg aagtttcctt
      301 ccgcgttctc aaagtcgata ggattcactt tgtgtggcat cgccgaagat cctatttctc
      361 ctgcttttgg tttctgcgtc acccagctgt cgcttatgta gcgccacatg tcctgcgaga
      421 aatcgatgag gatggtgttg atgcgacgca ctgtatcaaa aactgcgaca tagctatcgt
      481 ggcactctat ctgcgtcgta tacagattcg cctcaagctt atacgtgcgc tcaacgttta
      541 agctgtcaat aaatttactt gtgaatgcca tccaatcaac ctcagggtat gcaatagcat
      601 gcgcgttcca gttaccggac gcaccattca gttttgcctg tatcttgatg ttcttaagag
      661 aggtgagagc agtagcgata cgttcggaaa acaccttcat ctctttacca aacgtggtcg
      721 gtgacgcgga ctgaccatgc gtgcgcgcaa gcatgggagt ggccgcatag gtacgcgcaa
      781 gcgtgagcaa tccttcttgc actgcgacga ttcgcggaac gagcacgctc tcaagcgcgt
      841 ccgagagcat gagcgcgtat gcgatgttat ttacgtcttc tgaggtgagc gcaaagtgaa
      901 cccactctgc cttttcttca agggtagtgc cgcgaatacg ctgcttgatg tagtactcca
      961 ccgctttgac gtcgtgccgg gtcgcaggta tcccttctac cccttcagtc tcaatctttt
     1021 taacgagcgc agcatccgcc tcagaaactg ctgcaatacg tgccagaata tcttcttcgg
     1081 cttttgaaag agtgaacggg agctttcgtt cacgagcgag cgccataagg taggcgcatt
     1141 ccaccccaac gcggtaccgg ataaggccgt actcgctcat atagggcgcg aggcgctcta
     1201 ccttcgagcg gtaacggcca tcaagcgggc ttatcgccgt gagttcgttt gttgccatac
     1261 ttacttctta ctcttttcca taagcatcgc aactcgctca tatttatctt caatagttgc
     1321 catgtcttcc ccgttgcgga atgattgctt actcacatcc ttccagtcct tatcacgcag
     1381 ccgccagctg tcgttatcaa tgacgtcagc aataacaagg cgaccatccg tagtcacccc
     1441 aaactcaatc ttgaagtcca caaacttaaa gtcatgttct gcgaagaagg cttcaagcgt
     1501 gagaaacgta tttcgtatca tagcggtcat ttctgctatg gcgtcagtgg aaggaagcac
     1561 cttactgctg tctatcgtgc gcccgaggct tgccgcaggg tcagtgctcg gttttttgga
     1621 gagtgcgaga ttccaggtgg cgtcttcggg attggggatg aatgggtcct cctcaccctt
     1681 taacggatcg agtccttcga cgagcgtctc acccgcagca ctcttcaagc ttcccttcgt
     1741 tgttttcaaa aaatattcaa tgacgggttc ctcaaagcgg tgcggcacat ctccttcctg
     1801 tttgaattcg ggatgtcgat tcaggtagct gccgacggcg tagcgacgcg ctacgacttc
     1861 aagcgggacc atagtgcatt tctcagaaag gaattcagta ggagaaagtt gtcgaacgaa
     1921 ggcagtagga atacctgcag cgttcaacat ttcaaacacg cgagcgttaa cgatatttga
     1981 atactctgct tttttgttaa attgcttcgt aaactctgga ttatcaaacg ctgtgatgtc
     2041 gtttttatac tcaacgatgg cacgggtagg ttcacccggc gcttcgtata ttcttttcgt
     2101 cttcccttca gcaatcagcg ggccgcgagg agaggctggc atagcttcag gtgtttccat
     2161 actagatatc gaactcaagc gtctgccctg gcttttcagt tccgcgtgag acgagagtga
     2221 cgcccggctc cttcgttatc ttcccagcaa tcttcgcctc tataccaaac gcctgtgctt
     2281 ccttcaccac cttctcaggt tcaggggtaa tgagtgccat accctgcccc atgttccatg
     2341 cgcggtacgc atcatgatcg gagatttttc ctagcttctg gcagtgtgcc atcacttcag
     2401 gaggatcaaa gagatcggtg agttttgcgc caagcccgga aggacgtaag acgcgcatca
     2461 tcttctcagg aacaccgcca ccggtgatgt gcgcgacgcc gtgaatggtg cacgagcctt
     2521 ccgtctcaaa tccgccatgg agcgcaacga cgcagcgtga atagataact gatggggtga
     2581 gagcggcaat accgagcgtg gactcaccga atgctgcctc atgccagttc tcaccatatt
     2641 cctctttgaa gattttccgg atgagtgacc agccgtttgc tctaaaccca tgctcccgaa
     2701 gcatcaccac agaatctcct gcttttattt ctttgccggt gaagagtttt tctttgcgcg
     2761 caaaccacac acacgcagca ccccagtgat acgggaagtc accgaagcca gtcatcaagc
     2821 ttcccatctg tgctatctcg ccgttaatga cggcaacgtt tgcttcgtta gcggcagcca
     2881 catatccctt cgccagctcg cggactatcg ggaggtagca gtcatcggtg ccgagtgtct
     2941 ttatgtcgag gtttgatccc acgagtacgg gttccccacc gcgaagtacg gcgtcgtcac
     3001 acaccatggc aaacaggtcg tgcgcgatgg tgtcgtgtat accgacacgt tctgcaatct
     3061 ctaccttcgt gcctgccgta tcaaatccta cagagaagaa cgtgccttcg gggaggttgg
     3121 ctgctgacac cattttgata ccagcaaagt catcaaaagg tgcgactatc tgccctatga
     3181 gtccgtcccg gttcttaaac gtttccttgg aggcttcata cattattttt gatgcttgtg
     3241 cttcgatgtc gacatcgact ccggcaagcg cgtaggcgtt tggtttcttg tcgtccatat
     3301 cagttctctt taagatactc ctcgtagccc attgtttcca gcttttgggc cttggcgagt
     3361 acttcgtctt ttatcttctc tttgtacgcg cggaccaagt ccataatttc tgtgtctccg
     3421 gttcctaata tagaggcggc cagtatgccg gcattcttag ctccattcac cgcaacggta
     3481 gcaacaggaa ttcctggtgg catctgtgcg atagagagaa gcgaatcgag gcctttcact
     3541 gccttggtgg taatgggaac gcctatcaca gggagcggcg tcatggaggc ggtcatcccc
     3601 ggaagatgtg ctgcgcctcc tgcccccgcg ataatcactt tgatgccacg acgctctgca
     3661 ttggttgcat attctacaag acgctcaggg gtccgatgag cggagacgac ggtgagctca
     3721 tacatgatgc cgagatcatc gagtagcttt gccgcttcct gcatgaccgg aagatcggag
     3781 tctgagccca tgatgatccc taccgctgca ttcatggtca gatgctaaca cgacgccacg
     3841 cgtcctccgc ttttgctctc gcttcaagaa gtgagtttgc aacggcagtg atgtgcccca
     3901 tcttccggtc tggttttgtc tctttcttcc cgtatatatg gatagccacg ccaccgagcg
     3961 cttttgcatc ctcaactcca accggggtcg cagggccagt acgctcacca agaatattaa
     4021 gcatgacggc agcgggtgcg atgagttcgg tgctaccaag ctccataccc atcacggcac
     4081 gtacatggtt ttcaaactgg ctcgttgcgc atccttctat cgtgagatgg ccagagttgt
     4141 gcacgcgggg cgcgatttca tttaccaaca cgtccccatc aaccataaac atttcgatag
     4201 cgaatactcc ggcaccagaa aatgactcaa gcacgtagtg agcaagtgtt tctgctttct
     4261 tacgaacact ttcttttacg ggagcaggtg caagcacttc aagacaaata tgacgttcgt
     4321 ggaccgtttc aaccacggga taggtacgta tgtcgcccgc aaccgtgcgt accgccacta
     4381 ccgcaagttc cttctcaaag gagacgaatc cttcggcata gagattggtg ccgagcttcg
     4441 cgaaggcagg agcaatatca gcaggagtgt taattgtcgc gttaccgcgt ccatcatacc
     4501 caccactcct tgctttcaga aggaagggat agcccagtac ttcccctgca gcacgtgcgg
     4561 cttcctcgtc tggtgcatct tgataagggg ctaccggaat accgcgctca gaaagaaagt
     4621 tcttttgcgc gagcttatct ttaattaacg cgagcgtctc aggcatagga tgcaccggaa
     4681 gtccttgcgc tgcgagttcc gcaagggcgt cagcatttgc tgactctatt tcaaacgtaa
     4741 gcacctctgc ttctttggcg aatgcgagca cggttgcatg atcatgaaag tctcccacaa
     4801 cctgacgcgc aaccgcggat gctgggcact caggcgttgg atcaagtact acgcattcaa
     4861 taccgagtgt ctttgctgcc tgcgccatca tgagtgcaag ctgaccaccc ccgacaatac
     4921 ctaatgtttt catgagaaat actgaactgc gttacggaac actgccatgc cgtcggcgta
     4981 taccggtacc ttctttcctt cgcgctcgag ctgttcacga agcacggtcc agtcagggcg
     5041 ctgctcgaag aacgtagcgc ggtctgggtg gggcatgagg ccgaggatgc gtccctcttt
     5101 tccggtgaca ccagcaatgt cacgcatagc tccgttcggg ttatacggaa gtccctggta
     5161 ctcgctcatt tctccggcaa catatttgag cgcgactctg ccggtccctt caagcgtgtc
     5221 gaggttcgct tcagtatcca cgtagcgacc ttctccgtgc ccgataggaa gcgagacctt
     5281 tgtaatacct ttcaaccacg gcgtgtctcc cactacttcc atatctaccc agcggcacga
     5341 gtagcgcgct gattcgttcg tgatgagagc acccggaaga atgccggcgg cagtgagaat
     5401 ctggaatccg ttacaaatac ccgccattaa ggtgtctcgt tcaaggaatt tagtgagttc
     5461 gtcacccaaa tgatgcttca cgcgtgcgcc gtatgctttc ccagcgcccg tgtcgtcacc
     5521 atacgagaag ccacccggga acatcgctat ctgatacttc gcgagcatgg aaggattctt
     5581 cgccacgtct tcgatatgaa cgatgtctgc cgtagcgccc gcaagggtga ggccagtctt
     5641 cgtttcctct tcgcagttga ggccgtatcc agaaaagatg agtgcgcggg gagtagccat
     5701 attaaaagtc gttatacgga atgtgatatg cctcctggag tgcatcaagc ggaagcgttg
     5761 ttgaaacgct gcccgtttta atgcgaagtg tcggctcatc ctctaccgtt cctatctttt
     5821 taactgacaa atctttcatt gctgcgagga atgctttttc tttttgtggt gcaaccgtca
     5881 caaggaagcg tcccgtgctt tctgagaaga gtgcagtatg cgcatccccg gtgaacgaaa
     5941 cggttgcgcc aagcccaccg gcaagcgcca tcttaccgag cgcgacggca acgccacccc
     6001 gagagacggc gagcgctgat gacatgagtc gctttccgga agcctttgag aatgctttgt
     6061 ataccgagac attctctttt gcatctgtta ccggcacccg tcctgacata aaggtac
//
LOCUS       DAORIN010000029         6067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2453_length_6067_cov_4.362941, whole genome shotgun sequence.
ACCESSION   DAORIN010000029 DAORIN010000000
VERSION     DAORIN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2453_length_6067_cov_4.362941"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PK109_01760"
     CDS             complement(<1..960)
                     /locus_tag="PK109_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24298.1"
                     /translation="MTTEPTVMAPAPVVPPTHHSTHKGLLITLGILILISLAVGAAWY
                     LRSGTSSQEPVTFRVLENGTAAWYTLSGDGMSLSTDTVPSWYTEAPASVTAGTFSPAA
                     AGNGSVVALSSTGLESIDAANTKHASLITRAIKEWSSSVVVADASLAVLFNTATNAFD
                     VFSLDSERPTVLSYEGSIAAPELFYGVGALPGNRVVVRVSENQFDLYEVHGGTASLVR
                     SLMLAEAAPAETSFFSIPVAYAWTYGTPLTSTSASTAGSTRCTGAVVASLGGTTGTGA
                     MPSIQTMAWNYMGAQSGLPANYNNGSYCIQAVTTITTGGGGGGG"
     gene            1037..2032
                     /locus_tag="PK109_01765"
     CDS             1037..2032
                     /locus_tag="PK109_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRH24299.1"
                     /translation="MKSFDFLAVGDIVTEPFIRLKEASVHCNIDSENCTISMRWGDKI
                     PYESAEVCHAVGNSPNAAVAAARLGLTTGIATDIGNDDIGKGDLAQLKKEKIATDHVR
                     IHKGMVSNYHYVLSYESERTILVKHEEYPYRFPNIKAPRYLYLSSLAANSLAYHKEIE
                     AYLEKNPNVYLTLQPGTFQMKLGVTELKGLYARANLLVINKEEAARILGLPESGQEIK
                     DLLQKTRAFGPKAVIITDGRAGAYAYDGAEMLHIPMYPDDREPKERTGAGDAFASTVT
                     AAMALGKTFREALLWGPINSMAVVQEVGAQKGLLTRAALEAFLAKAPASYAVTSL"
     gene            complement(2027..2380)
                     /locus_tag="PK109_01770"
     CDS             complement(2027..2380)
                     /locus_tag="PK109_01770"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRH24300.1"
                     /translation="MKYWIKRFSHPIRGLRYAFTKDFAIRFELIILGIVGLPGAYLLF
                     GPFNAREMLLLIFCWFFIMVAEMQNSAIEIALDQVHPEHHEAIGRSKDLASAAVVWAA
                     VFGFIAFFFVLSGKL"
     gene            complement(2395..3372)
                     /locus_tag="PK109_01775"
     CDS             complement(2395..3372)
                     /locus_tag="PK109_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRH24301.1"
                     /translation="MINPAAKLSAKVFADDIEKKPTRDGFGLGTVEAGKENPNVVVLS
                     ADLGDSTRAAWFHKEFPDRYIEVGVAEQNLATVAAGMAAVGKIPFIASYAAFSPGRSY
                     EQIRTTAALNHLPVKIGGMHAGIQTGPDGATHQMLEDIALMRVLPNMTVMVPADSEEG
                     RKCIVAAAKLPGPCYIRFGRAAEPLFTTPETPFEIGKATTLWESKDPKVAFFTTGSVA
                     YRTLQAAKLLEEEGIGSLVLHFHTIKPFDDAAAVAAAKRVERIITVEEHQVAGGFGSA
                     VAECLSDKHPMKITRMGIQDVFGQSGEPDELMDFYGLSPRHIADEAKKS"
     gene            complement(3369..4202)
                     /locus_tag="PK109_01780"
     CDS             complement(3369..4202)
                     /locus_tag="PK109_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRH24302.1"
                     /translation="MALTEAEVLTIEEKAREVRASIIRMLVAAKSGHTAGPLGMTDIF
                     STLYFHTLVHDPKNSDWEGRDRVILSNGHIVPVRYATMAHAGYFPVEEAETTLRKLGS
                     RFQGHPEREKLPGMETTSGPLGSGVSQAAGMAYALRMDGKKNRIYCLTSDGEHQEGNT
                     WEGIMFAGKYRLANLTQIIDRNFIQIEGNTEDIMPLEPLADKYRAFNWHVIEIDGHNI
                     RQIHEAIEQAKAVADMPTLILANTIPGKGVKEIEGDYKWHGKPPTPEEGERFIKEILS
                     V"
     gene            complement(4224..4940)
                     /locus_tag="PK109_01785"
     CDS             complement(4224..4940)
                     /locus_tag="PK109_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24303.1"
                     /translation="MSIVIPAVLVPSRKVLDETLARLDGLYDLVQVDIVDGRFVGPPT
                     WPYSEPAELSVLEPDGQFPYLGHFKFEVDLMVENPEATVGKWVEAGAAKILVHMESVR
                     NMQQIVDELRENYGHEKGFAPDLLSLGVALNIDTDTSVIEPYIDAIDYVQFMGIAKIG
                     VQGQPFDTRVLRKIEAFKKTHPAMPIQVDGGVSLTTAPDLLSAGVTNLCVGSALLKTP
                     DLVATMHAFEEVAEQYGRYR"
     gene            complement(4947..5495)
                     /locus_tag="PK109_01790"
     CDS             complement(4947..5495)
                     /locus_tag="PK109_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRH24304.1"
                     /translation="MTPSSARGFTLIELLMVVAIIGILSTVVLGAMQDARLKAADAAV
                     RQQAVQLRNLLEQERTNTGSYAAIKSGGVGTPGPWIAVNGGCGTFTGQFAASAADVCK
                     KLVAAASTGGRCGTACVYFQSTSPNSATAYSIMAYLPYAAKKAAQDGLTPSLRYLCIG
                     SSGNQSIADGAAWTEDGCQNNP"
     gene            complement(5492..5719)
                     /locus_tag="PK109_01795"
     CDS             complement(5492..5719)
                     /locus_tag="PK109_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24305.1"
                     /translation="MTVEFDEAVPSGQTRPAPKPSFFTSLIIKTGIVKTPGGAQIVMG
                     IIALALLALTLFQFKHVTTQAPLPTPEQIAL"
     gene            complement(5774..>6067)
                     /locus_tag="PK109_01800"
     CDS             complement(5774..>6067)
                     /locus_tag="PK109_01800"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRH24306.1"
                     /translation="GGGSGNGEAMAANRIPGARAAVFHGRTGAVSAIDITGAAGASDG
                     YDIVRLSRKHNDANMLSLGFRFLSEEDCKEAVRIFLETSFEGGRHQNRVDKLG"
BASE COUNT         1380 a         1689 c         1634 g         1364 t
ORIGIN      
        1 tccaccacca ccgccgcccc cagtagtaat agtggtcact gcctgaatac agtaggagcc
       61 gttgttgtag tttgccggca gcccgctctg agcacccatg tagttccatg ccatggtctg
      121 aatagacggc attgctcccg tgccggtcgt accgccaagc gaagccacca ccgcacccgt
      181 gcagcgggta cttcctgcag tcgacgcgct cgtagatgtg agcggtgtgc catacgtcca
      241 tgcgtacgcg acaggaatgg agaagaatga tgtttcagca ggtgccgctt cagcgagcat
      301 aagcgagcga acgagtgatg cagtacctcc atgaacctcg tacaaatcaa actggttttc
      361 actgacacgg actacgacgc gattaccggg aagtgcccct acgccataga acaattctgg
      421 cgctgctatt gagccttcgt aggagagaac ggtcggacgc tcactatcaa gcgagaatac
      481 gtcgaacgca ttggttgccg tgttgaaaag cacggcaagc gaagcatcgg caaccactac
      541 cgatgagctc cactctttga ttgctcgcgt tatgagtgat gcatgtttcg tattagctgc
      601 atcaatcgat tcaagccccg tggaagacag cgcaactact gaaccgttcc ctgcggctgc
      661 tggagaaaag gttcctgccg taacacttgc cggagcctcc gtataccagg aaggcacggt
      721 atcagtcgaa aggctcatgc cgtcccctga gagcgtgtac catgctgccg taccgttctc
      781 taggactcga aaggtcactg gctcctggga ggacgtaccg cttcgcaagt accaggcagc
      841 gccaactgct aaggaaatca gtatgagaat gccgagcgta ataagaagcc ctttatgagt
      901 agaatgatgc gtaggcggta cgacaggcgc aggcgccata acggtgggtt cggtagtcat
      961 gccttccatg atagcaacca gcgcttcatg acctatatgc gcgtgtgcac aaccaagatg
     1021 ctagacttga gagcgtatga aatcctttga cttccttgcg gtgggcgaca ttgttaccga
     1081 accctttatc cgtctcaaag aggcgagtgt gcactgtaat atcgatagcg aaaactgcac
     1141 catctccatg cggtggggcg ataagattcc gtatgagtca gccgaggttt gtcatgcggt
     1201 cggtaactcc ccgaacgcag cggtagctgc cgcacgtctt ggtcttacga ctggcattgc
     1261 aaccgatatt gggaacgatg atatcggcaa aggcgacctc gcacagttga agaaggaaaa
     1321 gatagcaacg gatcatgtac gcatacataa aggaatggtg agtaactacc actacgtact
     1381 ttcgtacgaa agcgagcgca cgattctcgt aaagcatgag gagtatccgt atcgcttccc
     1441 gaacatcaag gcgccccgct atctctacct ctcttcgctt gccgctaatt cccttgcgta
     1501 tcacaaagag attgaagcgt acctagaaaa gaacccgaac gtatacctca ctcttcagcc
     1561 gggcaccttc cagatgaagc tcggagtcac ggaactgaag ggtctctatg cgcgcgctaa
     1621 ccttctcgtc attaataagg aagaggcggc acgtattctc ggccttcctg agagcggtca
     1681 agaaataaag gatctcctcc agaagacgcg cgccttcggc ccgaaggcag tcatcatcac
     1741 ggacggacgc gcaggcgcct atgcgtatga cggcgctgag atgctccata ttcccatgta
     1801 tccggacgac cgcgaaccaa aggaacgcac cggcgcaggc gacgcattcg cctcgacggt
     1861 gacagccgcg atggcgctcg gcaagacatt ccgtgaagcg ctgctctggg gacctattaa
     1921 ctcaatggcg gtagtacagg aagtgggcgc acaaaaagga ctgctgacgc gtgcagcgct
     1981 cgaagcattt cttgcgaagg caccggcctc ctacgccgtt acctccctat agttttccgc
     2041 tcagtacgaa aaagaaagcg atgaagccga acaccgcggc ccatacgacc gcggctgatg
     2101 cgagatcctt gctgcgccct atcgcctcat ggtgttccgg gtgtacctgg tcgagcgcta
     2161 tctcgatagc ggaattctgc atctcagcga ccataatgaa aaaccagcag aagataagca
     2221 ggagcatctc gcgtgcatta aagggaccga acaagaggta cgcacccgga agacccacaa
     2281 taccgaggat aatgagctcg aatcgtatgg cgaagtcctt ggtgaaggcg tacctgaggc
     2341 ctcggatagg gtgggagaat cgcttaatcc agtatttcat ggacgagaag cacgttatga
     2401 cttcttcgct tcatctgcga tatgccgcgg cgaaagtccg tagaagtcca tgagctcatc
     2461 aggttctcct gactgcccaa acacatcctg tatgcccatg cgcgtaatct tcatcggatg
     2521 cttatctgag agacattcag caaccgcgct tccaaaccca cccgctacct gatgctcctc
     2581 taccgtgatg atgcgctcta cgcgcttggc ggcagccacc gcagccgcgt catcaaatgg
     2641 ctttatcgta tggaaatgaa gtaccagaga accaatgcct tcttcttcaa gaagctttgc
     2701 cgcctgaagc gtgcgatagg cgacagagcc agtcgtgaag aaggcgacct taggatcttt
     2761 tgattcccag agcgtcgttg ccttccctat ctcaaacggc gtttcgggag tcgtgaacag
     2821 aggttctgca gcgcgcccga aacgtatata gcaggggccc ggaagctttg ctgctgccac
     2881 gatgcactta cgtccttctt cagagtctgc cggcaccatt accgtcatgt tggggagcac
     2941 acgcatgagt gcgatgtcct cgagcatctg gtgggtcgca ccatcaggtc ctgtctgaat
     3001 acctgcatgc atgccgccaa tcttcaccgg aagatgattt agggctgcgg tagtgcgaat
     3061 ttgttcgtag ctgcggcctg gagaaaatgc cgcatagctt gcgatgaaag gaatttttcc
     3121 gacagcggcc atacccgccg ctaccgttgc aagattttgc tctgctacgc ccacttctat
     3181 atagcggtct ggaaactcct tatggaacca cgcagcgcgt gtggaatcgc cgaggtcagc
     3241 ggagagcacg accacatttg ggttctcttt ccccgcctca acagtaccaa gaccaaaccc
     3301 atcgcgagtc ggtttctttt caatgtcatc cgcgaatacc ttcgcagaga gttttgcagc
     3361 gggattgatc atacggaaag tatctctttg ataaagcgct caccttcttc aggcgttggt
     3421 ggcttgccgt gccatttgta atcaccttca atttctttga cgcccttacc aggaatggtg
     3481 ttcgcaagga taagggtcgg catatcggca actgctttcg cttgttctat cgcttcatgt
     3541 atctggcgaa tattgtgtcc atcaatttca atcacatgcc agttgaaggc ccgatactta
     3601 tccgcaagcg gttcaagcgg catgatgtct tccgtgttgc cttctatctg tatgaagttg
     3661 cggtcaatga tttgcgtgag gttcgcaagg cgatatttac cggcgaacat aattccctcc
     3721 catgtattac cttcctgatg ctcaccgtca gacgtaaggc agtagatgcg attctttttt
     3781 ccatccatcc ggagcgcgta cgccatgcca gcagcctgtg agacgcctga gccaagcggg
     3841 ccagacgtcg tctccatccc tggaagcttc tcgcgctcag gatgtccctg gaaacggctg
     3901 ccgagctttc gaagcgtggt ctctgcttct tctacgggaa agtagccggc gtgtgccatc
     3961 gtcgcatacc gcacgggaac gatgtgaccg tttgaaagaa tgacgcgatc acgtccctcc
     4021 caatcagaat tctttgggtc atgtacgagt gtatggaaat agagcgtgga gaaaatatcg
     4081 gtcataccca aaggtcctgc agtgtgtccc gactttgctg caaccagcat ccggataatc
     4141 gaagcgcgca cctcgcgcgc cttctcttca atagtgagca cttctgcttc ggttaacgcc
     4201 ataccgctga ttatacctaa cctttatcga tagcgcccat actgctcagc gacctcttca
     4261 aacgcatgca tggtcgccac aaggtcgggg gtcttgagga gtgctgagcc gacgcagagg
     4321 ttggtgacgc ctgcggaaag gagatcgggg gcggtagtaa gggatacgcc gccatctacc
     4381 tgtatcggca tagcgggatg tgtcttctta aacgcttcaa tctttctgag tactcgtgta
     4441 tcaaacggct gcccctgtac cccaatcttt gcgataccca tgaactgcac ataatcaatg
     4501 gcgtcgatgt aaggctcaat gaccgaggta tccgtatcaa tgttgagcgc gacgccgagc
     4561 gagagaaggt ccggcgcaaa tcctttctcg tgtccgtagt tctcccggag ctcatcgacg
     4621 atttgctgca tgttccgcac gctttccata tgtacgagta tctttgctgc acccgcctct
     4681 acccatttcc ccaccgtcgc ttccggattc tccaccataa gatccacttc gaatttgaag
     4741 tgtccgaggt acgggaactg tccgtcaggc tcgagtacgg aaagctcagc aggttccgag
     4801 tacggccagg tgggaggccc tacgaaccgc ccatcgacga tgtcgacttg gaccaagtcg
     4861 tagagaccgt caagccgggc caatgtttca tctagcacct ttcgtgaggg aacgagcacg
     4921 gccgggatca cgatgctcat gatgcgttag ggattattct gacagccgtc ctcggtccat
     4981 gccgcgccgt ccgcgatact ttgatttccg ctcgatccta tacacaaata gcgtagtgat
     5041 ggggtgaggc cgtcttgtgc cgctttctta gcggcgtacg gaagatacgc catgatgcta
     5101 tacgccgttg cagagttcgg actcgtcgat tggaagtaca cgcaggcagt accacagcgt
     5161 ccgccggttg aggccgctgc aacaagtttc ttacacacat ccgcggcgga cgctgcaaac
     5221 tgacccgtga atgtgccaca gccgccgttt actgcaatcc agggccctgg agttccgacg
     5281 ccgccactct tgatggctgc atagcttccc gtattggtac gctcttgttc gagcaagtta
     5341 cgaagctgca ctgcttgctg gcgtactgca gcgtccgccg ccttgaggcg cgcgtcctgc
     5401 atcgcgccaa ggaccacggt tgaaagaata ccgatgatgg cgactaccat caggagctcg
     5461 ataagggtga agccgcgtgc ggacgaaggg gtcatagagc gatttgttca ggagtcggaa
     5521 gcggcgcctg cgtagtgaca tgtttaaact ggaaaagggt gagtgccaag agtgcgaggg
     5581 cgatgatgcc cattactatt tgcgctcctc ccggagtctt cactatcccg gtcttaatga
     5641 taaggcttgt aaaaaaagag ggcttggggg ctgggcgggt ctggccgctc ggcacggctt
     5701 cgtcgaattc aacggtcata ggggaaagta taccgtgccc agctgattcc ctatccccgc
     5761 cctgtagata cggctagccg agcttatcga cccggttctg atggcgtccg ccttcaaacg
     5821 aggtctcaag gaagatgcgc acggcctctt tgcagtcctc ctctgagaga aatcgaaagc
     5881 ctagggagag catattcgcg tcgttgtgct tgcgagaaag ccgcacgatg tcgtacccgt
     5941 cagaggcgcc tgcagcaccg gtgatatcta ttgcggaaac tgcgcccgtg cgtccatgaa
     6001 agaccgcggc gcgtgcgccc gggatgcggt ttgcggccat cgcttccccg ttcccgcttc
     6061 ccccgcc
//
LOCUS       DAORIN010000030         6036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2470_length_6036_cov_4.570808, whole genome shotgun sequence.
ACCESSION   DAORIN010000030 DAORIN010000000
VERSION     DAORIN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2470_length_6036_cov_4.570808"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PK109_01805"
     CDS             complement(<1..522)
                     /locus_tag="PK109_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRH24307.1"
                     /translation="MEIRNIAIIAHVDHGKTTLTDAMLRQTGAVVEGATMDSNKIEQE
                     RGITIYSKNASLEYKGTKINIVDTPGHADFGSEVERVLRSIDSVLLVVDAQEGPMPQT
                     RFVLKKSLELGLKPIVVLNKIDKPAADPARAEEQVLELFFELGANDAQANFPVVYAIG
                     RDGVAGKTLNDMPS"
     gene            complement(590..2371)
                     /locus_tag="PK109_01810"
     CDS             complement(590..2371)
                     /locus_tag="PK109_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRH24308.1"
                     /translation="MEKKTGARDGLYTKETIRIYAKAAGKYPFLLTGVIVGGIFVQFV
                     QLIQPLFLRDFFNLIAAGNQDPASIAQMTWILTVVAGLLFINWVCIRLHIYSLVYLDA
                     RVLRDTYQLAYAYLIRHSYRFFSNQFAGTLTRRVGRFSKSFETIFDGIVMQFLPAAIF
                     IGGALVVISLQNAFLGLLYGGWVVLILTVQVVLIRANNPLRAARAAQDSRVTGALADA
                     IGNQNTIDLFSGTDYEEKRFKAVHADWYRAVLKTWNMDELGWSILGILMVLINIALLF
                     GTFHFWRQGLLTVGDFVLIQSYLLSTFDRVFSINREIRNFNEAFADAGEMVEILKTPH
                     EITDVPNAPALLVHKGAIEFKNVNFTFTGERTILSDFDMAIKAGEKVALVGPSGAGKT
                     TVTKLLLRFYDVTGGEILIDDQNISQVTQNSLRDAIAFVPQEPILFHRTLMENIRYGR
                     RDATDEEVIAAAKKAHCEEFISRTPLGYETFVGERGIKLSGGERQRIAIARAILKDAP
                     ILVLDEATSSLDSESESFIQDALAKLMEGKTVIAIAHRLSTVMKMDRIIVIEEGKVVT
                     TGTHDELVKHKGGLYKKLWEIQAGGFI"
     gene            complement(2391..3407)
                     /gene="mltG"
                     /locus_tag="PK109_01815"
     CDS             complement(2391..3407)
                     /gene="mltG"
                     /locus_tag="PK109_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRH24309.1"
                     /translation="MLQRHIEFLKEKVFQSWGRHIFYAFLVLAALLIIFVSLHAALFG
                     PPGTDTTRENLIVQPDETLEEIAADLEAKGFVRHAFVFRFAYTVARTDDSIRPGGYEL
                     ARDMDAWTVAATLGRAPYLSWITIPSGVRKEEIAEILTESLGWTRAQHDEWLAATETS
                     ADLTEGVYFPDTYLIPSDQPPAQIAARLRGRFEEAFAPYATIAADKDIPWTDVVNLAA
                     LVEKEAAKNDKALVAGILWNRINKNMKLQVDATLQYMVGSEEDWWPTPNVDDKTSTST
                     FNTYKYEGLPPHPIANPSLESIAAVLAPQKTACLYYLHDSRGVIHCSTNYKAHVANVN
                     RYLK"
     gene            3538..4671
                     /locus_tag="PK109_01820"
     CDS             3538..4671
                     /locus_tag="PK109_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRH24310.1"
                     /translation="MSKNKLAGVVLALALLVGGATAAPAYAATQAEQLATLIAQVRAL
                     QAQLAALTGETGSFSMNLTLGSSGPDVTRLQSWLIGKGYGIPAGATGYFGAQTQSALA
                     RYQAAVGIVPASGYFGPITRARVNSMTVVTPIPTTPTPSDTSDRTEAADAIDDLDAAI
                     DDAQDDIDDAEDDGEDVDDANDLLDDARDLLADAEDAFDDEDYDEVMDLVDEAMDVID
                     EALDEANVDSDNEGDAEVSLRTATTQSVSGADNDYATFKIRLTLNAFGDDMYLPQDAD
                     DAVTYQIEDASDGSVVGSATTMSATFSSSADEVGDYYIIDENGDEEFTLTVTFDPTPG
                     DEGKSYRLQLLTIEYNDSENAPDSTWSTHGDSEFQTPSTYISD"
     gene            4771..>6036
                     /locus_tag="PK109_01825"
     CDS             4771..>6036
                     /locus_tag="PK109_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRH24311.1"
                     /translation="MQKILSALRGAFRRVIRAITPLWVRFRALPRWAQALIVAVIIGL
                     LFFISSLLGSAPAEVSNAGRAVTLRSVAELSGAATGESVVGSVRARSEAEIRAEAAGT
                     VRRVNTTIGASVPAGFIIAELENDAQAAAVLQAEGAYDAAVASRASVSPVDVQTSARN
                     AYRSTFETLDAALENQVDTVFGEITPYGPKLLINPIGTDASVLPRERHRIDGLMDAER
                     TTLASAATTDSEALLAGIERTARQVATFIDTLVETANATGSGASATQITALTGARAAV
                     NGALTSITAARASLRSGATSSTASVDAGVKSALGTLRLAQASLEKTRLRAPIAGTINF
                     LPIRIGDYVGNLDHVATVAQNGALEIVSFVSEDARAGLEVGAKAVVEGTYPGTITSIS
                     PALDPVNKQIEVHVSVDGTTGLVNGQSVRL"
BASE COUNT         1347 a         1606 c         1694 g         1389 t
ORIGIN      
        1 gcttggcata tcgtttaggg tcttgccggc aaccccgtca cgaccgatgg cgtacacgac
       61 tgggaagttt gcctgcgcat catttgcacc gagctcgaag aagagctcga gtacctgttc
      121 ttcagcacgt gccgggtccg ctgctggctt atcaatcttg ttcaacacga cgataggctt
      181 gagaccgagt tcaagggact tcttgagtac gaaacgggtc tgaggcatcg gaccttcctg
      241 agcgtcgacg acgaggagca ctgagtcgat ggaacggagc acgcgctcca cttctgaacc
      301 gaagtcggcg tggcccgggg tgtccacgat gttgatcttg gtgcccttgt attccaaaga
      361 ggcgttcttg gagtaaatgg tgataccgcg ctcctgctcg atcttattcg agtccatggt
      421 cgccccctca acgacggctc ccgtctggcg cagcatagcg tccgtcaagg tagtcttgcc
      481 gtggtccacg tgagcgataa tagcgatgtt tcggatttcc ataatgtaaa aaggcccgcg
      541 ggccgttatg cagagagaat acgttgaaaa cgaaggagcg tcaatcgctc taaataaacc
      601 cgcctgcctg tatctcccac agtttcttgt agagtccgcc tttatgcttc acgagctcgt
      661 cgtgggtacc ggtggtcact accttccctt cttcgataac gatgatgcga tccatcttca
      721 tgacagtaga gaggcggtgg gcgatggcga tcacggtctt tccttccatg agttttgcaa
      781 gggcatcctg gatgaacgat tccgattctg aatccaaaga ggaagttgcc tcgtcgagca
      841 ctaatatagg agcgtcttta agaatggcac gtgcgatagc aatgcgctgg cgctcacctc
      901 ccgaaagctt gatgccgcgc tctcccacaa aggtttcgta cccaagcggg gtgcgtgaaa
      961 taaactcttc gcagtgtgcc ttctttgcgg cagctatcac ttcttcgtca gtggcatcgc
     1021 gcctcccgta gcgaatgttc tccatcagcg tgcgatggaa aaggattggt tcctgtggca
     1081 caaaggcaat agcgtcgcga agtgagttct gcgttacttg tgaaatattc tgatcatcaa
     1141 tgagaatctc tcctcccgtg acgtcataga agcgaagcag gagtttggtg acggtagtct
     1201 tgccggcgcc tgagggacct acgagtgcta ccttctcccc cgccttaata gccatatcaa
     1261 agtcggaaag aatggtgcgc tccccggtaa aggtgaagtt aacgtttttg aattcaatag
     1321 ctcccttatg cactaagagc gctggtgcgt tcgggacgtc agttatctcg tgtggtgtct
     1381 taagtatttc caccatctct ccggcgtcag cgaatgcttc gttgaagttt cgtatctcac
     1441 ggtttatgga gaacacgcga tcaaaggttg aaagcaggta cgattgaatg agaacgaagt
     1501 ctcccaccgt gagaagtccc tggcgccaga aatggaacgt gccgaacagg agcgcaatat
     1561 tgatgagcac catcaagata ccgagaatac tccacccgag ctcatccatg ttccatgttt
     1621 taagcaccgc tctataccag tccgcatgta ccgctttaaa tcgtttctct tcgtaatcgg
     1681 ttcccgagaa cagatcgatg gtgttctggt ttcctatggc gtcggcgagt gctcccgtca
     1741 cccgactgtc ttgtgcggcg cgcgccgcac gaagcgggtt attcgcgcgt atcagtacga
     1801 cctggacggt aagaatgagc accacccacc cgccatacag aagaccgaga aatgcgttct
     1861 gcagggagat gacgactaac gcgccgccta tgaagatagc ggcaggcaag aactgcatga
     1921 cgatgccatc aaatatagtc tcgaatgatt tcgagaagcg cccgacgcgg cgcgtgaggg
     1981 tgccggcgaa ttgattcgag aagaaccgat aggaatggcg gatgagatac gcgtaggcga
     2041 gttggtaggt gtcgcgcagc acgcgtgcat caagatagac gagcgagtag atatggaggc
     2101 gtatacagac ccagtttatg aagaggagtc ctgcgactac cgtcagtatc caggtcatct
     2161 gtgcaataga cgctgggtcc tgattccctg ctgctatgag gttaaagaag tcgcgtaaga
     2221 agagcggttg gataagctga acgaactgta cgaagatgcc gccgacgata acgccggtca
     2281 ggaggaacgg atactttccc gcggccttcg cgtagatgcg tatcgtttct ttcgtgtaga
     2341 ggccgtcgcg cgcgcccgtc ttcttttcca tacgcctcaa tatagcactg ctatttgagg
     2401 tagcggttca cgttcgcgac gtgcgctttg tagttcgtgg agcaatgaat gacgccgcgc
     2461 gagtcatgga ggtagtagag gcacgcggtc ttttgaggcg cgagtactgc agcgatggat
     2521 tcaagcgagg gattggctat cgggtgcggc gggagtcctt cgtacttgta cgtattaaag
     2581 gtagaggtcg acgtcttatc atccacgttt ggggtgggcc accagtcttc ttcactaccc
     2641 accatatatt ggagtgtagc gtccacctga agcttcatat tcttattgat acggttccag
     2701 agaatgccgg cgacgagcgc cttgtcgttt ttggctgctt ccttttcgac aagtgccgct
     2761 aaattcacga cgtccgtcca tggaatatcc ttgtccgcgg caatggtagc gtagggcgcg
     2821 aacgcttctt caaagcgtcc gcgaagacgg gcagcgattt gtgcgggtgg ctgatcactc
     2881 ggtatgagat aggtgtcagg gaaatacacg ccttcagtga ggtcagcgct tgtttccgtc
     2941 gctgcaagcc actcgtcatg ctgtgcgcgc gtccatccga gggattcggt gagaatttcc
     3001 gctatctctt ctttgcgtac gccgcttgga atggttatcc acgacaggta cggcgcacgg
     3061 cccagagtgg cggcaaccgt ccaggcgtcc atatcacgcg ccagctcata tcctcccggg
     3121 cgaatagaat cgtcggtgcg tgcaacggta tacgcaaaac ggaacacaaa cgcatgtcgt
     3181 acgaatcctt tcgcttcaag gtcagcggcg atttcttcaa gggtctcgtc aggctgcacg
     3241 atgagatttt cccgcgtcgt gtcagttccc ggaggaccga agagcgcagc gtggagcgaa
     3301 acaaagataa taaggagggc ggcaagcacc aggaacgcat agaatatatg tcgtccccag
     3361 ctctggaata ctttttcctt gagaaactca atatggcgct gcaacattta tatgcagtat
     3421 accctggtcg ttaatcgtat ctccatgaac aatacggata cttgaggatg ctggtaagca
     3481 aatgctgcct acggcgtcta tactagagtg agtgctaacc attaattcct atcaaccatg
     3541 tctaaaaata aattagccgg agtagtgctc gcgcttgccc ttttggtagg aggcgctacc
     3601 gccgctcctg cctatgccgc cactcaggct gagcaattgg cgacgcttat tgcgcaggtg
     3661 cgcgcactcc aggcgcagct tgctgctctt acgggagaaa ccggatcctt ctcgatgaat
     3721 ctcacgctcg gttcttcggg acctgacgta acccgcttgc agagctggct catcgggaag
     3781 ggctacggca ttcctgccgg agccaccggc tacttcggtg cgcagacaca gagcgcactc
     3841 gcgcgctatc aggccgcagt cggcattgtt cctgcgtccg gatacttcgg tccgattacc
     3901 cgtgctcgag tgaactcgat gacggtcgtt accccgatac caacgacgcc aacgccaagc
     3961 gatacgtctg accgcacgga agcggcggac gctatcgatg atcttgatgc agcaatagat
     4021 gacgcacagg acgacattga tgacgccgag gacgatggcg aagacgttga tgacgccaat
     4081 gacctccttg atgatgcgcg tgacctcttg gctgacgccg aggacgcgtt tgacgacgag
     4141 gactatgacg aagtcatgga cttagtcgac gaggcgatgg acgtcattga tgaggccctt
     4201 gatgaggcaa atgtcgactc tgacaatgaa ggggatgctg aagtcagcct tcgcactgca
     4261 accacgcagt cagtgagcgg cgcagataac gactacgcga ccttcaagat acgcctcacc
     4321 ttgaacgcat tcggtgatga tatgtacctc ccgcaggatg cagacgatgc cgtcacctat
     4381 cagattgagg acgcatcaga tggttctgtg gtggggtctg ctaccaccat gagtgctacc
     4441 ttctcttcaa gtgctgacga agtaggtgac tactacatca ttgatgaaaa tggtgacgag
     4501 gagttcaccc ttaccgtaac gtttgaccca acaccgggcg acgagggtaa gagctaccgt
     4561 ttgcagcttc tcacgattga gtacaacgac tctgagaacg ctccagacag cacgtggagc
     4621 acccacggcg atagcgagtt ccagacgccg tctacctaca ttagcgacta ggaaagacgt
     4681 tcgttcgaac ttttgacaag agccgcccct cggggcggct cttgtcatat aagtatggaa
     4741 atatgcagta aatatgctat agttcgggca atgcaaaaga tactttccgc gctccgcgga
     4801 gccttccgtc gtgttattcg tgcgattacg ccgctctggg tgcggttccg cgcgcttccc
     4861 cgctgggctc aggcgcttat cgttgctgtc atcatcggtc tcctcttttt tattagttcc
     4921 ctcctgggaa gcgccccggc tgaagtaagt aacgcaggcc gcgctgttac gcttcgctcg
     4981 gttgccgaac tctcgggtgc agccaccgga gaaagcgtgg tgggttcggt acgcgcccgc
     5041 tctgaagctg agatacgcgc agaagcggca ggcactgtgc gccgcgtgaa cacaacgata
     5101 ggcgcgagcg ttccggcagg cttcatcatc gcggaacttg aaaacgacgc gcaggcggct
     5161 gcagtcctcc aggcagaagg cgcgtacgac gctgcggttg cgtcgcgcgc ctccgtgtct
     5221 ccagtagatg tacagacatc agcacgtaat gcctaccgtt caacgtttga aacgcttgat
     5281 gcggcccttg agaatcaggt ggatacggtt tttggtgaga taacgccgta cggaccaaag
     5341 ctcctcatca atccgattgg cactgatgca agcgtgctgc cgcgcgaacg acatcgtatc
     5401 gacggcctta tggatgctga acgcacaacg ctcgcttctg cggcaactac tgattcagaa
     5461 gcactccttg ccggcattga gcgtaccgcg cgtcaggtgg ctaccttcat cgataccctc
     5521 gttgagaccg cgaatgcgac aggctctggc gcaagtgcaa cgcaaataac cgcactcacc
     5581 ggagctcgtg ctgcagtgaa tggcgcactc acctctatta cagctgcgcg ggcgtctctt
     5641 cgcagtggcg ctacctcctc taccgcttcg gttgatgcag gggtaaagag tgcactcgga
     5701 acccttcgcc ttgctcaagc atcacttgag aagacgcgac tacgcgcacc gatagcaggc
     5761 accataaact tcctcccgat tcgtattggt gactatgttg gaaacttgga tcatgttgct
     5821 accgttgcac agaatggtgc gcttgaaatt gtgtccttcg tttcagaaga tgcacgcgct
     5881 ggtcttgagg taggtgctaa agcggtggtt gaaggaacgt acccgggcac catcacgtct
     5941 atttcgccgg ccttggaccc agtgaataag cagatagagg tgcacgtgtc agtggacggc
     6001 accacgggac tcgtgaacgg tcagtcggtg cgcctt
//
LOCUS       DAORIN010000031         5935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2527_length_5935_cov_5.788946, whole genome shotgun sequence.
ACCESSION   DAORIN010000031 DAORIN010000000
VERSION     DAORIN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_5935_cov_5.788946"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..503)
                     /gene="mscL"
                     /locus_tag="PK109_01830"
     CDS             complement(117..503)
                     /gene="mscL"
                     /locus_tag="PK109_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRH24312.1"
                     /translation="MNSFLKEFKTFALRGNALDLAVGVVIGAAFNGIVTSLVNNVLTP
                     PLGMLIGGVNFSDLVLPIGGEASIQYGVFIQALISFIITAFALFLLVSFINKFLRKAD
                     KEPKAPAPKSEELVVLEEIRDALKQK"
     gene            complement(536..611)
                     /locus_tag="PK109_01835"
     tRNA            complement(536..611)
                     /locus_tag="PK109_01835"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(576..578),aa:His,seq:gtg)
     gene            complement(675..872)
                     /locus_tag="PK109_01840"
     CDS             complement(675..872)
                     /locus_tag="PK109_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24313.1"
                     /translation="MKQRLMKSLSVSLEWRLFAFIITELFFWATTGHLWQATILALQL
                     QLILLLGHFGWYFFRESGKQV"
     gene            complement(975..1056)
                     /locus_tag="PK109_01845"
     tRNA            complement(975..1056)
                     /locus_tag="PK109_01845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1020..1022),aa:Leu,seq:gag)
     gene            complement(1105..1650)
                     /locus_tag="PK109_01850"
     CDS             complement(1105..1650)
                     /locus_tag="PK109_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRH24314.1"
                     /translation="MAGVITPVKTEERTKLGIRPGDTVRVHQKITEQKKGKAANKKDT
                     TANVYRTQVFEGLVLAVKHGSEAGGSFTVRATLSGIGVERIFPLFAPFIEKIEIVKRS
                     KVRRAKLYFIREKVAREVRRQLRNMRMMNVSTDDFAEKEADVAAEVVEALDEAVAEAM
                     PEEVVAEAEAPAEVAEEKAAA"
     gene            complement(1675..2424)
                     /locus_tag="PK109_01855"
     CDS             complement(1675..2424)
                     /locus_tag="PK109_01855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRH24315.1"
                     /translation="MNRMELPIIYEDDAIVAVNKPAGVMTHPDGKGTEETVSDWFAAH
                     YPASADVGETQRLQDGTEIRRPGIVHRLDRETSGVLVLAKTPEAHASLKEAFQNREAK
                     KTYLAFTYGVPKERKGMIDFAIGRSRQDFRLRSAQPKAKGTLRDAITRYEVIADNGTH
                     SLMKVMPETGRTHQIRVHLKATHHPVVGDPLYAPNRKADLGFDRLGLHAFMLDVPLPN
                     GERKLIQAPMPDDLVQGVGEFPGAEAIIASL"
     gene            complement(2449..3135)
                     /gene="uppS"
                     /locus_tag="PK109_01860"
     CDS             complement(2449..3135)
                     /gene="uppS"
                     /locus_tag="PK109_01860"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRH24316.1"
                     /translation="MSERGVPTSIGFILDGNRRWAKEHNLPQLEGHRRGMEKVKEILR
                     FAMDAGVKEVCVYAFSTENWKRSPEEVDYLMKLFEEFCDRWAEELQKEGGRLRFIGQR
                     ERFSASLQQKMQRAEKVTAQGTKATLIVALSYGGRAEIIAGVNELLKRGVKEVDETTF
                     EKVLWSNDIHDPDLIIRTGGEQRLSNFMTWRSVYSELFFTKTKLPAFTKEEFNAILDE
                     YTARDRRRGR"
     gene            complement(3128..3403)
                     /locus_tag="PK109_01865"
     CDS             complement(3128..3403)
                     /locus_tag="PK109_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRH24317.1"
                     /translation="MERMTAIVSGRVQLVMYRDFSCRNARGLGLVGTVQNLPDGTVRV
                     IAEGEREKLDRYALKLWQGSLLSHVTNVAVSWSPATGEFTKFKIQYE"
     gene            3445..3520
                     /locus_tag="PK109_01870"
     tRNA            3445..3520
                     /locus_tag="PK109_01870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3478..3480,aa:Val,seq:tac)
     gene            complement(3563..3646)
                     /locus_tag="PK109_01875"
     tRNA            complement(3563..3646)
                     /locus_tag="PK109_01875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3610..3612),aa:Leu,seq:cag)
     gene            complement(4374..4448)
                     /locus_tag="PK109_01880"
     tRNA            complement(4374..4448)
                     /locus_tag="PK109_01880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4413..4415),aa:Arg,seq:tct)
     gene            complement(4467..4778)
                     /locus_tag="PK109_01885"
     CDS             complement(4467..4778)
                     /locus_tag="PK109_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24318.1"
                     /translation="MNLPPQLTAFLKRFSSHDGTKHLDPTRDWLLLLALVIIALIASV
                     VWNAWFFFIALEEESTSQSAPVEATPDTNSVEKAQALFELRAAEEARYRNEYRFIDPS
                     R"
     gene            complement(4782..5294)
                     /locus_tag="PK109_01890"
     CDS             complement(4782..5294)
                     /locus_tag="PK109_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24319.1"
                     /translation="MKPPIIHLGIAALLAILAIGGYVLWYGHVQALDAEAATLAGEIA
                     VKDADRVRSVSARSAEAELALQEAFIASHLVATADIVVFLEQLEKTGRDFGATLQVAS
                     VTGGEPATDGRISLSLSITGPFNSVMRTLGAIETGPYAGMVQDLSLNGSAEGEWTATG
                     VFVIGTMSTP"
     gene            complement(5291..5845)
                     /locus_tag="PK109_01895"
     CDS             complement(5291..5845)
                     /locus_tag="PK109_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24320.1"
                     /translation="MSAELTNLLPSERIRAFRQAYFYRLGTALLLAVAVVVVIHAALL
                     APSYLYLNDTVALERAHLNELAEKLASSGEGEMNARLARLEQDASRILTLSGAPSASA
                     AIRTVLAVPRGGITLTGITFHTPSPDGRLTLTGMAATREDLRRYALALSELPFAKSAD
                     LPLSAYAKERDIPFTITLTGPLAI"
BASE COUNT         1423 a         1576 c         1461 g         1475 t
ORIGIN      
        1 ccatggtgca ggctaccggt tccgggccac ctgtgcgctc tagtacattc cggtagatga
       61 atcctgcaaa gccaataacg atgatgagca caatgccgcc gataagtgat ttcatattat
      121 ttttgtttta gagcatcccg tatttcttca agtaccacca actcttctga ctttggcgca
      181 ggcgcttttg gttccttatc tgctttgcgg agaaacttat taatgaacga tacaagcaag
      241 aagagcgcga acgcagtaat gatgaatgag atcaacgcct ggataaatac gccgtactga
      301 atagaggctt ccccacctat cggaagcacg aggtcactga agttcactcc gccaatgagc
      361 atccccagag gaggcgtgag tacgttgttg acgagcgacg taacgatacc gttaaatgcg
      421 gcgccgataa cgacgcccac tgctaaatcg agagcgtttc cccgaagggc aaacgtctta
      481 aattctttaa ggaatgagtt catgaggaaa gtatagcaaa actacttttc tcaattgggg
      541 cgacttgtgg ggctcgaacc cacgaccgcc tggaccacaa ccaggtgctc taccagctga
      601 gctaaagccg ccatgtacgc acgttcactg tttcgcactt ataccatgcg atttgatgcg
      661 gaggaagcgg acggttacac ctgcttcccg ctttcgcgga agaaatacca tccgaaatgt
      721 cctaagagga ggatgagttg cagttggagc gcaaggatcg ttgcctgcca cagatgtcct
      781 gtcgtcgccc aaaagaaaag ttctgtgatg atgaacgcaa acaaacgcca ctcaagagaa
      841 actgatagtg acttcatgag tcgttgcttc atgcatttat catgcagtgg aataacttcg
      901 gggacaacct gtgtacagtg cgtggaaaac gagaggatat acaaaaagcg ccgtgtggcg
      961 ctttttgtgt ttagtgccca ggagaggact cgaacctcca ccccgttaag gactaccacc
     1021 tcaaggtagc gtgtatacca atttcaccac ctgggcaggt gtaacgccca ctatacgcgt
     1081 cgaacctcgt cttttcaacg aggcttacgc tgctgccttc tcttctgcta cttctgcagg
     1141 agcttccgcc tctgcaacta cctcttctgg catcgcctca gcgacggcct catcaagcgc
     1201 ctctactact tctgctgcaa catctgcttc cttttctgcg aagtcgtcgg tggagacgtt
     1261 catcatgcgc atgttgcgga gctggcggcg tacttcacga gccaccttct cacggatgaa
     1321 gtagagcttt gcgcggcgta cctttgaacg ctttacgatc tcgatcttct cgatgaacgg
     1381 agcaaagagg gggaagatac gctcgacgcc aatgcctgaa agggtggcac gaacggtgaa
     1441 ggaacctcct gcctcggagc cgtgcttaac cgcaaggacg aggccttcga agacctgggt
     1501 gcggtatacg ttagccgtgg tgtccttctt gttagctgcc ttgcccttct tctgctcggt
     1561 aatcttctgg tggacacgaa cggtatcgcc cgggcggatg ccgagcttcg tgcgttcctc
     1621 tgtcttaacg ggagttatta cgcctgccat agttgcgcaa ctgtagcata gggcctagag
     1681 agaggcaata atcgcctcag ctccgggaaa ttcccctacc ccctgcacga ggtcatccgg
     1741 catcggtgcc tggatgagtt ttcgttcacc gtttggtaac ggcacgtcga gcataaaggc
     1801 atgaagaccg agtcgatcaa accctagatc agcttttcgg ttcggtgcat acaacggatc
     1861 ccccactacc gggtggtgag tggcttttaa gtgcacgcga atctggtggg tgcgtccggt
     1921 ctccggcatc actttcataa gcgagtgcgt tccgttgtca gcaattactt cataacgcgt
     1981 aatagcgtcg cgaagcgtgc cttttgcttt tggttgcgca ctccgcagac ggaagtcctg
     2041 gcgggaacga ccaatggcaa aatcaatcat gcctttgcgt tctttgggta ctccgtacgt
     2101 aaacgcaaga taagttttct tcgcttcccg gttttgaaat gcttctttaa gagatgcgtg
     2161 cgcttcggga gtctttgcaa gaactaaaac accagaggtc tcacgatcaa gacggtgcac
     2221 gatgccaggg cggcgaatct ccgtgccgtc ctggagccgc tgcgtctctc ctacatcagc
     2281 agaggccggg tagtgcgccg caaaccagtc gctcaccgtc tcttcagttc ccttcccgtc
     2341 cgggtgcgtc ataacgcccg cgggcttgtt gaccgcaacg atagcatcat cttcgtagat
     2401 tatcgggagt tccatgcgat tcaccttatc ctgctcggcg tacttatact agcggccccg
     2461 tctacgatcc cgcgcagtgt actcatcaag tattgcgttg aactcttctt tggtgaatgc
     2521 ggggagcttg gtcttcgtga aaaacaactc gctatacact gaacgccacg tcatgaagtt
     2581 agagagacgc tgctctccac ccgtacggat gatgagatcg ggatcgtgta tatcgttact
     2641 ccagagcacc ttttcaaacg tagtctcgtc gacttctttc actccccgtt taagaagctc
     2701 attaacgccg gcaattatct cagcacgtcc tccatacgaa agcgcgacga tgagagtcgc
     2761 tttggtaccc tgtgccgtaa ccttctcagc acgctgcatc ttttgctgga gtgatgcact
     2821 gaaccgctcg cgctgtccaa taaaccgcag acgccctcct tccttttgaa gctcctcagc
     2881 ccatcgatca cagaattctt cgaacagttt cataagatag tccacttctt ccggactgcg
     2941 cttccagttc tctgtcgaga acgcgtacac gcacacctct tttactcctg catccattgc
     3001 gaagcgcagt atctctttca ctttttccat accgcgccga tgtccttcga gctgcggcag
     3061 attatgttct ttcgcccatc gacgattacc gtccaagata aagccgatgg aggtcggcac
     3121 gcctctttca ctcatactgg attttgaact tcgtgaattc accggtagca gggctccatg
     3181 agaccgcgac gttcgtgacg tgggaaagaa gtgatccttg ccaaagtttg agtgcgtagc
     3241 ggtccagttt ttcacgctct ccttcagcga taacgcgcac agtgccgtcc ggcaggttct
     3301 gaacagtgcc tacaagacca aggccgcgcg catttcggca gctaaagtcg cgatacatca
     3361 cgagctgtac cctcccagac acgattgcgg tcattcgttc catcctcttt caagaatacc
     3421 gcatttcagg tatggttctt ggacgggcga ttagctcagt tggtagagca actcatttac
     3481 actgagaagg tcacaggttc gagtcctgta tcgcccacca gacccatgca aaaaccggcc
     3541 aaatggccgg tttttgctgt agtgcccgag agaggactcg aacctccacc cccttacgag
     3601 gacgacgacc tgaacgtcgc gtgtctacca atttcaccac ccgggctttg ctcgattatg
     3661 cccatagatt tcttaatctt caaatatttt ctttcgaggc gagggcaggt cctattttta
     3721 tacatctccg tacttagtaa aattgcttct cttttactgt atttcaactg aagacaagag
     3781 gagttgtgtt tatggtctac agaacctctt attttaatcc tccgtatcag attttttctt
     3841 agccaggaga tatatttcgg acttgccgaa gcaaaagaaa tatagaatcg gtagcttttt
     3901 ctaaacacgg aatcataccg agaataacta catccatctc cgtcaaaata gcctcttaag
     3961 aaatcgaaga aatactgatc tggaatcaat acggaagaaa gagtttttga ttttgcgggt
     4021 gttagcccaa tggactcgag aaaacgataa aagagcacgt ctccgaactg cgtgtgatat
     4081 gcaggattcc ctgcaccaga aaacttgatg cttatttttg tatcaagttt taaacattcc
     4141 ttgaatgtct caacttggtc tctatctttt gaagtgaaat cgatatgacg cccatctttc
     4201 gataaacacc catcggcagt cagcagtcca atcgcatatg caaagttgga agaccagatt
     4261 aagactatct tgcctttcgg ttttggacct gtcttcgcca tttctcaacc ttaactgaga
     4321 caattagcat ctatctgtta ttttgcacac aatcaacaca acttttcctt taatggtgct
     4381 cccaccagga atcgaaccta gatctacggc ttagaagtcc gctgctttat ccattaagct
     4441 atgggagctt taaaacagta ccggccttag cgcgacgggt caatgaaacg atactcatta
     4501 cggtatcgcg cttcttccgc agctctcagt tcaaagagtg cctgtgcctt ttctactgag
     4561 ttagtgtcag gagttgcttc tacgggcgca ctctgactcg ttgattcttc ttcaagagcg
     4621 ataaaaaaga accaggcatt ccatacgacg ctcgcaatga gtgcgattat gacgagcgca
     4681 aggaggagca gccaatcgcg cgttgggtct aagtgcttag tgccgtcatg gctactaaac
     4741 cgtttcaaaa atgccgtgag ttgtggagga aggttcatag gttatggcgt actcatagta
     4801 ccgataacaa acacacccgt tgcggtccac tccccttctg cggaaccgtt aagggagagg
     4861 tcttgtacca ttcccgcata cggtccggtc tcaatagcgc ccaaggtgcg catcacgcta
     4921 ttaaagggac cagtaataga aagcgacaat gagatacgtc catctgtcgc tggctcacct
     4981 ccggtcactg acgcaacttg gagtgttgca ccaaagtcgc gtccagtttt ctcaagctgc
     5041 tcaagaaata ccacgatgtc agcggtggca accaagtgag aagcaatgaa tgcttcttgc
     5101 aaagcgagct ctgcttctgc agaacgtgct gacacgcttc gcacgcggtc agcatctttc
     5161 accgcgattt ctcccgcaag cgttgccgct tccgcatcaa gcgcctgtac gtgtccgtac
     5221 cacagcacgt atccccctat cgcaagaatg gcgaggagcg ctgcaatgcc gagatgaatg
     5281 atcgggggtt tcatatggcg agagggcccg taagggtaat ggtgaacgga atgtcacgtt
     5341 ctttggcata tgccgaaagc gggaggtccg cactcttagc aaatggaagc tctgagagcg
     5401 cgagtgcgta gcggcgtaga tcttcacgag tcgccgccat gccggtaagg gtaaggcggc
     5461 catcaggtga aggagtgtgg aacgtgatgc ccgtaagcgt gataccaccg cgcggcactg
     5521 caagcaccgt acgtatagcc gctgacgcag acggtgctcc ggaaagcgta aggatgcgac
     5581 ttgcgtcttg ctcgaggcga gccagtcgtg cattcatctc tccttcccca gacgaagcaa
     5641 gcttttctgc aagttcgttt aagtgtgcgc gttcaagcgc taccgtgtca ttcaaataaa
     5701 gatacgaggg tgcaagaagc gctgcatgaa tcaccacaac caccgctact gcaaggagga
     5761 gcgccgtacc aagtcggtag aaataggcct gacggaacgc acggatacgt tctgacggaa
     5821 gaagattagt gagttcagcc gacatattct cgtagcgcta agccaatggc agtgccgtac
     5881 gcaagggact ccgtgtactc aagcggcggc aaccacttat ctcgcggcgc aaagt
//
LOCUS       DAORIN010000032         5741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2643_length_5741_cov_5.123461, whole genome shotgun sequence.
ACCESSION   DAORIN010000032 DAORIN010000000
VERSION     DAORIN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2643_length_5741_cov_5.123461"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PK109_01900"
     CDS             <1..243
                     /locus_tag="PK109_01900"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HRH24321.1"
                     /translation="IIVSGAGQAHIITPDMIKEGVVLIDAGTSGANGAVTGDIDPACA
                     TKASIMTPVPGGVGPVAVSCLFSNTARLFDAGKGLA"
     gene            272..1570
                     /gene="secD"
                     /locus_tag="PK109_01905"
     CDS             272..1570
                     /gene="secD"
                     /locus_tag="PK109_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRH24322.1"
                     /translation="MRFIPALGILILAGLLSYLVLTTNGPTDPHRVKLGLDLAGGTEL
                     IYKADTAAVLTDKQGALNSLREVIEKRVNVFGVAEPLVQLEKSSAVAGVVEDRLLVEL
                     PGVTDVKAAVDALGKTPLLEFKLVGGVVGDAVAYTPTELTGRYLKNAELQFGTGQSGG
                     GLANQPVIVLHFTEEGGKLFEQITTDNVGKQLAIFLDGEVISSPVIQEAIAGGTATIT
                     GSFTPAEGRDVVRNLNFGALPVPIELASSGTVGSTLGASAFEAGLIAAALGFALVALF
                     MIFWYRLPGLVAVVALAIYVVITIAIIKFLPVTLTASGIAGFILSIGMAVDANVLIFE
                     RMKEELRAGKGAHDAAATGFARAWPAIRDGHFTMIISSIILFWMGTSLVQGFALVFGF
                     GVIVSLFTAITVTQVFLKTILPESRNGVWNFLLSSGFHTK"
     gene            1573..2478
                     /gene="secF"
                     /locus_tag="PK109_01910"
     CDS             1573..2478
                     /gene="secF"
                     /locus_tag="PK109_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRH24323.1"
                     /translation="MLIIKYRAFFFWLTGLILAGAIGALVFFGLPLSIDFTGGSLVEV
                     AYQDTRPDVTALKERVAAAGFDDLSLRESGEKGITVRAHTLTPEEHNALLLSLSNNGA
                     EPVTELRFNSIGPSLGSELAIKALYAIGAVMLAIMFYIALVFRKVSRPVPSWGYGLVV
                     ILMLAIDIIVPAGFFAAYAHFTGAQADALFVVALLALLGYCVNDVIIVFDRVRERLKL
                     NLEKNVKEDFETTVGISIDQTLGRSINTSLTVALALLAIILFGAEATRDFALVMLVGV
                     VVGTFSSICRSAPLLIPIAKQFSKK"
     gene            2484..2849
                     /locus_tag="PK109_01915"
     CDS             2484..2849
                     /locus_tag="PK109_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24324.1"
                     /translation="MASLDHIFGWAGKPGEVEAKRQFEANLSAAIVNYNPIAVQELII
                     ERLTEGKKIANPYDFCRKIADDMNLLTGKDADPHALHDARPGTKGMRVLWEFLASVKP
                     KETVENLLTNIRSLKLETT"
     gene            2846..3406
                     /locus_tag="PK109_01920"
     CDS             2846..3406
                     /locus_tag="PK109_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRH24325.1"
                     /translation="MILGITGTIGAGKGTVVEYLVKEKGFTHYSVREAITEEILRRGL
                     PVTRPNLNEVATDLRRTHGPTHFGDHFRARAEQDGVKDYIIESIRNPKEAEKIKSIGG
                     FIIIVDADEHLRYDRVKGRGSHTDDVTFEEFQAHEAREMTTEDPSDPSFMDIAAVMRM
                     ADATIMNDGTLEELHAKIDEVLATLS"
     gene            3408..3755
                     /locus_tag="PK109_01925"
     CDS             3408..3755
                     /locus_tag="PK109_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HRH24326.1"
                     /translation="MAYEDQVPTNVPEKGFYYHYKHNPEGKVNNYAYEFLGVGHHTED
                     DCRPEDALLGVYRPLYEAFVYKNGKMFDVRPLGMWMEEVTKEGKTFPRFARITDASVI
                     AQLMKIRMEMYGE"
     gene            3758..4234
                     /locus_tag="PK109_01930"
     CDS             3758..4234
                     /locus_tag="PK109_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRH24327.1"
                     /translation="MPHIHEKIDFGADVYVVNQDAILLRFHEKYHFWCAPGGHIELNE
                     DPEEAAVREVKEETGLDVVLVGKRAPEFNDGDREVLPPRFVNRHRINETHEHVVFKYF
                     ATAATRYLQPAIGEKETDMRWFTRDELDDPKYDIRERVRSYAKAALDELASPKNPR"
     gene            complement(4655..5350)
                     /gene="pyrH"
                     /locus_tag="PK109_01935"
     CDS             complement(4655..5350)
                     /gene="pyrH"
                     /locus_tag="PK109_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRH24328.1"
                     /translation="MLRPKREYVVVSVGGSLIVPDGIDVSFLKNLRALVNRKVKEGFG
                     FYIIAGGGKLARRYQDAAKEVRGDLTADDADWMGIHATRLNAHLLRTLFLEDAQDRIV
                     KNPTRSVNTKYPVIVGSGWKPGNSTDYCAVMAAKKLGAKKLVNLSNIDYVYTADPRTN
                     PDAVKIERSNWADFRKLIPKDWDPGLSSPFDPVAAKEADAMKLEVAVINGAKLEELEN
                     YMDGKPFVGTVIA"
     gene            complement(5445..>5741)
                     /locus_tag="PK109_01940"
     CDS             complement(5445..>5741)
                     /locus_tag="PK109_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24329.1"
                     /translation="QERKEILEAIDGVDEVIITDHSLNDEDRSVCRALMAIRPHIFAN
                     GGDRHPDGDPVPEVLVCEELGIETVYNVGAGGKVQSSSELAKHAAEMRRSIDAR"
BASE COUNT         1319 a         1491 c         1470 g         1461 t
ORIGIN      
        1 attatcgtct caggtgccgg tcaggcgcac attattacgc ctgacatgat caaagagggc
       61 gtcgtactca tcgacgctgg cacaagtggc gccaatggcg cagtgaccgg cgacattgac
      121 cctgcctgcg ctaccaaagc ctccataatg actcctgtcc ctggtggggt ggggcccgtc
      181 gccgtgtcgt gcctctttag taatacggcc cgtttatttg acgcaggcaa gggtttggca
      241 taacggcacc taaggcgtac acttggccat tatgcgtttt ataccagccc tcggcatcct
      301 tattcttgcc ggccttctta gttacctcgt gcttacgacg aacggtccta ccgacccgca
      361 ccgcgtgaag cttggccttg atcttgctgg cggtacagag cttatttata aggcagacac
      421 ggctgcggtc ctcacggaca agcagggagc ccttaactcg cttcgcgaag tcatcgagaa
      481 gcgggtgaac gtgttcggtg ttgctgaacc actcgtgcag cttgagaaat cgagcgcagt
      541 tgcgggtgtg gtggaagatc gtcttcttgt tgaacttccc ggcgtcactg acgtgaaggc
      601 tgcggtagac gcgctcggca agacgccgct tcttgagttc aagctcgtag gaggtgttgt
      661 gggggatgcg gttgcgtata ctcctaccga acttaccgga cgctatctca aaaacgctga
      721 gcttcagttt ggtaccggac agtcaggtgg tgggttagcg aaccagcccg tcatcgttct
      781 tcatttcacc gaagagggcg gaaaactctt tgagcagatc accaccgata acgttgggaa
      841 gcagctcgcc atattccttg atggggaagt catctcgtct ccggttatcc aggaagcaat
      901 tgccggcggc accgcaacca ttaccggttc cttcactcct gcagaaggac gtgatgtagt
      961 gcgtaacctt aactttggcg cgctcccagt ccctatcgag cttgcgtcga gcggtacggt
     1021 aggttcaaca ctcggcgcct ctgcgtttga ggcgggcctt attgctgccg cccttggctt
     1081 tgcgctcgtc gcactcttta tgatcttctg gtaccgcctt cctggattgg tggcagtcgt
     1141 cgcactcgcc atctacgtcg ttattacgat agcgattatc aagttcctcc ccgttactct
     1201 tacggcttca ggcatcgccg gcttcattct ctctatcggt atggcggtgg acgcaaacgt
     1261 cctcatcttc gagcgtatga aggaagagct tcgtgcgggt aagggagcgc atgacgcagc
     1321 agcgactggc tttgcccgcg cctggcctgc gatacgtgac ggtcacttca cgatgatcat
     1381 ttcatctatc atcttgttct ggatgggtac ctcactcgtg cagggctttg cactcgtgtt
     1441 cggattcggc gttatcgtat ccctctttac tgccattacc gttacccaag tcttcttgaa
     1501 gaccattctc cctgaatctc gcaatggagt ttggaacttc ttgctttcaa gtggctttca
     1561 cacgaaataa ttatgcttat catcaaatat cgcgcgttct tcttctggct cacgggactc
     1621 atcctcgcag gagctattgg cgctctggtc ttctttggtc tcccgctctc tatcgacttt
     1681 acgggagggt ctctcgttga ggtagcgtac caggatacgc gtcctgacgt gacggcactt
     1741 aaggagcgtg tcgcagcagc gggcttcgat gatctttcac ttcgcgagag cggcgagaag
     1801 ggcattacgg ttcgtgcgca cacgcttact ccagaagagc acaacgcact tcttctctct
     1861 ctttccaata acggtgctga gcccgtgacc gaactacgct tcaactctat tggtccgtcc
     1921 ttgggctctg agcttgctat aaaagcgctc tacgctattg gcgcggtgat gctcgccatc
     1981 atgttctata tcgcactcgt gttccgtaag gtatcgcgcc cggtaccaag ctggggctac
     2041 ggactcgtcg ttattctcat gctcgctatc gatatcatcg tccctgccgg cttcttcgcg
     2101 gcctacgcgc acttcaccgg agctcaagct gacgcgctct ttgtggtcgc tcttcttgct
     2161 ctccttggtt actgcgtgaa cgacgtcatt atcgtctttg accgtgtgcg tgagcgcttg
     2221 aagttgaacc ttgagaagaa cgtaaaagag gacttcgaaa ctacggttgg tatctccatt
     2281 gaccagaccc ttggtcgctc gattaacacc tcgctcacgg tagcgctcgc gctccttgcc
     2341 atcatcctct ttggtgctga ggcaacgcgc gactttgcgc tcgtgatgtt ggttggcgta
     2401 gtggtaggta ccttctcttc aatctgccgc tctgctccgc tcctcattcc tatcgcaaag
     2461 caattcagca aaaaataaac accatggctt ctctcgacca tatattcggt tgggcaggga
     2521 aaccagggga ggtggaggct aaacgtcagt tcgaagcaaa cctaagcgca gcaatagtta
     2581 actacaaccc tatcgcggtt caggaactta ttatagagcg ccttactgaa ggcaagaaaa
     2641 tagcaaatcc ctacgatttt tgcagaaaaa tcgccgacga tatgaatctt cttaccggta
     2701 aagacgctga cccacatgct ctacatgatg ctcggccggg aactaagggt atgcgggtgt
     2761 tgtgggaatt tcttgcatca gtgaaaccaa aagaaacagt cgaaaatctt cttaccaata
     2821 tccgatctct taagcttgaa accacatgat tcttggcatc accggtacca ttggcgcagg
     2881 aaagggaaca gttgttgagt acctcgtgaa agaaaaaggt tttacgcact actcggtgcg
     2941 tgaagcaatt accgaagaaa tactacgtcg tggccttccg gttacccgac caaatttgaa
     3001 cgaggtagca accgacctac gtcgtaccca cggtccaact catttcggag atcacttccg
     3061 cgcacgtgca gaacaggatg gagtgaaaga ctacatcatt gaatccattc gtaatccaaa
     3121 agaagcagag aagataaaaa gcataggcgg ttttattatt atcgttgatg ccgacgaaca
     3181 tctccggtat gaccgagtga aagggcgagg gagtcacacg gatgacgtta cctttgagga
     3241 gtttcaggcg cacgaggcgc gagagatgac cacggaagat ccctctgatc cttcctttat
     3301 ggatattgct gccgtcatgc gtatggccga cgcgaccatc atgaatgatg gaacgctcga
     3361 agaacttcat gcgaagatag atgaagtact tgcaacactt tcttaacatg gcatacgaag
     3421 atcaggtccc cactaacgtc ccggagaaag gtttttacta tcactacaaa cataacccag
     3481 aggggaaggt gaataattac gcctatgagt ttttaggtgt tggtcatcat actgaagacg
     3541 attgccggcc cgaggacgct ttacttggcg tctatcgccc cctctatgag gcctttgtat
     3601 ataagaacgg gaagatgttt gatgtgcgtc ctcttggtat gtggatggag gaggtcacga
     3661 aagaaggaaa gacgtttccg cgctttgcac gtattactga tgcgtccgtt atcgcacagc
     3721 ttatgaagat tcgtatggaa atgtacggag aatagctatg ccgcatattc acgagaagat
     3781 agattttggt gcagatgttt acgtagtaaa tcaggacgcc attctcctgc gctttcatga
     3841 gaagtatcac ttttggtgtg ctccgggcgg gcacattgaa cttaatgagg atcctgaaga
     3901 agcagcagtg cgcgaagtaa aagaggagac agggcttgat gtggtattgg tagggaaacg
     3961 tgcgcccgaa tttaatgatg gagaccgaga agtgctgccg ccacgatttg ttaatcggca
     4021 caggataaac gagacgcacg aacatgtagt gttcaaatac tttgctactg ccgcgacaag
     4081 atatttacag ccagctatag gagaaaaaga aactgatatg cgatggttta ctcgagatga
     4141 gctggacgac cccaaatacg atattcgtga acgtgttcgg tcttacgcaa aggctgcgct
     4201 cgacgagctt gcgtcaccca agaatccacg ctaacctagc ctctatgcat cagttcagcg
     4261 cgtttacgta ttggtttacg aatccctcct gcggagagga ttgctttgat gcgcgctaac
     4321 tagccccact catcaaatca gccccctccg caaggagggg tttcgcgttg agcggaatca
     4381 cattgttcat tcgtgaggac actatgtcag acagacgatc tacatcttcc tacttcgttc
     4441 aagaaccaag cgcaccgcgc cactcgtcaa tgaaagacga gcgcgtcggc aggcaaagtc
     4501 cttgttgtga agacggcaag tccgtatcga cctacgtcga tggagtcaca atatactctt
     4561 gttcctcttg cggagatcag ctcaactacg ttgagtgttg atctaaaagc aggggaaggg
     4621 aagtaaaacc ggcaacgaaa gttgtcggtt tttcttatgc aattaccgta cctacaaacg
     4681 gtttcccatc catgtaattc tcgagctctt caagtttcgc accgttaatc actgctactt
     4741 caagtttcat ggcgtctgct tcttttgccg ctaccggatc aaatggggaa gagaggcctg
     4801 ggtcccagtc ctttgggatg agcttgcgga agtctgccca gttggaacgt tctatcttca
     4861 ccgcgtcagg gttcgtgcgt gggtctgccg tgtacacgta atcaatattg gaaagattca
     4921 cgagcttctt agcgccaagc ttctttgccg ccatcacggc gcaatagtca gtggagtttc
     4981 ctggcttcca gccagaaccg acgatgaccg ggtacttcgt atttaccgag cgtgtcgggt
     5041 ttttaacgat acggtcctga gcgtcctcaa ggaaaagggt gcgcagaagg tgcgcattca
     5101 ggcgtgttgc atggatgccc atccaatcag catcgtcagc ggtaagatcg ccgcgcactt
     5161 cctttgcggc gtcctgatag cggcgggcaa gctttcctcc accagcaatt atgtagaaac
     5221 caaacccttc cttcactttt cgattcacca atgcacggag attcttcaaa aaggagacat
     5281 cgattccgtc cgggactatt aaagaccctc ctacggacac gacgacatat tcacgcttag
     5341 ggcgcagcat aggcaaaggt ttgacggctc ctcgtattct acgcccctaa gccccatatg
     5401 caatattgac gggaagcgga gtcgggctgt attcgggagc ttggttatcg agcgtcaatg
     5461 cttcgacgca tttcagctgc gtgctttgcc agctccgaag atgactgtac ttttcccccg
     5521 gcaccgacgt tatatacggt ctctattccg agctcttcac atacgagaac ctcagggaca
     5581 gggtctccat caggatgacg gtcaccaccg ttcgcgaata tatgtgggcg gatagccatg
     5641 agcgcacgac atacgctcct atcctcatca ttaaggctgt ggtcggtgat gatgacttcg
     5701 tcaacaccat caatcgcttc aagaatctct ttacgctcct g
//
LOCUS       DAORIN010000033         5618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2724_length_5618_cov_6.040266, whole genome shotgun sequence.
ACCESSION   DAORIN010000033 DAORIN010000000
VERSION     DAORIN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_5618_cov_6.040266"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..2869
                     /locus_tag="PK109_01945"
     CDS             146..2869
                     /locus_tag="PK109_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24330.1"
                     /translation="MKYEADGHHTGGPGARKESASGIEELSEGVVIDQKVLAWIYKNV
                     HGKDVHAIATDQFLNKSRDNYGPPWLVRELVQNFVDHNETSPGTLNGVECIKTNLKDG
                     GVRFEIKGHWKYRDSTGVLSPHSEKPKDAKTAGGNGIGLKQTAIRFLRDFGVDTFEIH
                     GEQWAVKYRLARANVMNALLKQQMAVNQTPAVEVKHDWLIGEMEKTQNSGECRYIIET
                     KDPAVIAALDTFQTLGVSKDNEFLKNPDFKNRYGAIKWITPSAEFKEKMQGKRPLPKN
                     SDDDEIVRRPYGRLYINGQVMNYSSKGKTDETYWQGLDFMTLELDNIDYEMSVDRPPV
                     ASYLLPRYARDLVNSMTKEDILEQLRRSEPIWSLMMTGGWAPIVESMVLRLKQYGVSS
                     ANDFAALFPGKKYLAANADFSSEQRRKFVEEDGYTLCPGFFSDIGMQAVSTLLSESEQ
                     IRRNSKANTYDVSKAMEKLAAETGIQVLAPAYEVKTASQFAALIKERLIPSAVAFDFD
                     DSTNSMRITFAQDCPDALILNPLASPEGSSSETLMHALRGCIRSGLATRILSLDTCPM
                     VSNKELSGIYSINEISIFNPPLFMKTKRMVGDKSWTADFSINETYYSTIKAAMNGVEL
                     PEPPEPIRTSPSQTLPSGAKASQRASVPEQARPIVRKAEMIPAPEDIPGLSEAVDSLE
                     KIAEKKGKKKIGSLIGQYLSLRGKEEAKKTLASGAGYLTGISLREILAGTSEADIAPA
                     IDRRSEMSRRLQSAIDRLDEDDRVDNFEIVLEPSEGQLAQLSLLREYVHRTTGAAFSN
                     DLFVFTGDGAKGVNISQEAIGVHAEVLNVSFGEALEVMFHELAHNAYMNHSSEFMSQM
                     MSLSMRSQENVMELLEKSERREKLTPEERFVLTVRKEWSALQTGKKVKRRK"
     gene            complement(2878..3231)
                     /locus_tag="PK109_01950"
     CDS             complement(2878..3231)
                     /locus_tag="PK109_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRH24331.1"
                     /translation="MKTYTGGCHCGALRYEADVALDGAMSCNCSYCGKKGFLLTFVAP
                     AAFRIIAGKESDLTEYLFNKKHISHLFCPTCGVQSYGRGKGMDGSEMIALNVRCLDDV
                     DPETLTITKFDGKSL"
     gene            complement(3311..3607)
                     /locus_tag="PK109_01955"
     CDS             complement(3311..3607)
                     /locus_tag="PK109_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24332.1"
                     /translation="MAHKAKKNPGKTINRIDPVPGWEGLLKSLPRNSTSEARAGFADA
                     LDASRTKKKRRILSRYGQDVAAVVPLEDVALLELLSKPKHRKVLRALTEELKKM"
     gene            complement(3670..4122)
                     /locus_tag="PK109_01960"
     CDS             complement(3670..4122)
                     /locus_tag="PK109_01960"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRH24333.1"
                     /translation="MALHETIKAGIKDAMKARDEVKLRTYRSLVTMMTNEVVAKKRKP
                     DEFLTDDEALAVLKRAANQRKDSIQQFDTAGRSELSEPEKLELAVIEALLPAQMPREE
                     IEAVVKAKIAEMGADKSKAGQLTGAVMKELKGQADGGVVKEVVDSLLS"
     gene            4163..>5618
                     /locus_tag="PK109_01965"
     CDS             4163..>5618
                     /locus_tag="PK109_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRH24334.1"
                     /translation="MDMRPWHALPATEALTAFNISVEKGLDAEEAARRLSTGANTIRA
                     GASDTLLKQFWRQIKSPIAIVLLLAALATVLIAHYADAFIITLALMVNVAIGIFQEGK
                     AGDAFKALAKEEAPRAQVIRNGLKREIPASEAVPGDIVVLTAGAKAPADIRLIETHGL
                     SMNEAALSGEWLPSEKNSDAVGMDAPLVERTGMAYAGTLVVTGAATGIIVATGNDTEL
                     GKIASELGNRQGETPLQKDIKGIARLLLIIVALVIALVVVLALTHGITFGETLLIAIA
                     IAVAAIPEGLPAAVTVVLALGMERILKQGGLVKSLLAAETLGATSIILTDKTGTLTEG
                     RMKAAGFVTLSGTTEEADGENARLMLRAAVLASDGYIEEVDESTTPGDEIIARGRPME
                     QAILLAGLEAGFSERALRTEFPRVDELHFTSARRFGGMLVAEHGKHVTYLTGVPELFL
                     DHARRSLGARGGERAFTKENAAYFEEALLRAACEG"
BASE COUNT         1464 a         1361 c         1458 g         1335 t
ORIGIN      
        1 gcgagagggt ttcaggagct gaagtgggtt cgtgcgaggg taagtaattc ctcaccgact
       61 cagaaaacac gccacccggc gtgttttctg ttttaccaac actcctcgcg ttataaaaaa
      121 caggtaagga cggtacactg tcttcatgaa atatgaagca gatgggcacc acactggagg
      181 gccgggtgcg cgcaaagaat ctgcttcggg aatagaagaa ctgtcggaag gagtggttat
      241 tgatcaaaaa gtgctcgcgt ggatatataa aaatgtgcac ggaaaggatg ttcatgccat
      301 cgctaccgat caattcctta ataaatcgcg cgataactat ggtcctcctt ggctcgtacg
      361 tgaactcgta cagaacttcg tcgaccataa cgagacctcg cccggcacac ttaatggtgt
      421 cgaatgtata aaaactaatc ttaaggatgg gggagtacgc tttgaaataa aagggcattg
      481 gaaataccga gactcaacgg gtgtcctttc tccgcactcg gaaaagccta aagacgcaaa
      541 gactgctgga ggaaacggta taggactcaa acagactgcc attcgtttcc tccgtgattt
      601 tggcgtagac acctttgaaa tacatggcga acaatgggcc gtaaagtacc gacttgcgcg
      661 tgcgaatgtg atgaatgcac tccttaagca acaaatggca gttaatcaaa cgcccgccgt
      721 tgaggtaaaa cacgactggc tcatcgggga gatggagaag acacagaact caggggagtg
      781 ccgctacatc attgagacca aagatcctgc ggttattgca gctctcgata cattccaaac
      841 tcttggagtg tcaaaggata atgaatttct taagaaccct gattttaaaa atcgatacgg
      901 tgctatcaag tggattactc caagcgctga attcaaagag aagatgcagg gtaaaaggcc
      961 actacctaaa aatagtgacg atgacgagat agttcgtaga ccatatggac ggctttacat
     1021 aaatggacaa gttatgaact acagttcaaa aggaaaaaca gatgagacgt actggcaagg
     1081 gcttgatttc atgacacttg aactggataa catcgactat gaaatgagtg tcgatcgtcc
     1141 accagttgcg tcctatttgc tcccacggta tgcgcgggac ttggtcaatt cgatgactaa
     1201 agaagatatc ctcgaacaac ttcgtcgctc ggagcctatc tggtcgctca tgatgaccgg
     1261 cgggtgggca cctattgtcg aaagtatggt gctacgttta aaacaatatg gcgtttcaag
     1321 tgccaatgat tttgctgcac tattccctgg taagaaatat cttgccgcaa atgcggattt
     1381 tagtagcgaa cagcgacgta aatttgttga agaagatggc tacacgttat gtcccgggtt
     1441 cttcagtgat ataggaatgc aagccgtgag tactcttctc tctgagtctg agcaaatacg
     1501 tagaaacagc aaagcgaata cgtatgacgt tagcaaggca atggaaaagc ttgctgcaga
     1561 aaccggcata caagtgctcg ctcccgcata cgaagtaaaa actgcttctc aatttgcagc
     1621 gcttattaaa gaacgtctca taccatcggc agttgctttc gattttgacg atagcactaa
     1681 cagtatgcgc attacatttg ctcaggactg tcctgatgcg ctcatcttga atccattggc
     1741 aagcccagaa ggttcttcgt ctgaaacact catgcatgca cttcggggtt gcattcgttc
     1801 agggctcgcc acacgcattc ttagtcttga tacgtgcccc atggtatcga ataaggagct
     1861 ttccggcatc tatagcatca atgagataag tatctttaat cctccgctat ttatgaaaac
     1921 aaagcggatg gtaggtgaca agtcatggac tgcagatttc agtattaatg agacatacta
     1981 ttcgaccatt aaggcagcga tgaatggagt agaacttccg gagccgcccg aacctattcg
     2041 tacctcgcct tcgcaaacgc tcccatcggg tgcaaaagct tctcaacgtg cttcggtacc
     2101 tgaacaagct cgaccgatcg ttagaaaagc agaaatgatt cctgcacccg aagacatacc
     2161 cggactttca gaagcggtcg attctcttga aaagattgcg gagaagaaag gaaagaaaaa
     2221 gataggaagc cttattggac agtacctttc gttacgaggt aaagaagaag ccaagaaaac
     2281 cctcgcctca ggcgccggct atcttacggg aatcagttta cgagaaatac ttgcaggaac
     2341 gtcggaagct gatatagcac cagccattga taggcgttca gaaatgagtc gtcgtctcca
     2401 gtccgccata gatcgacttg atgaggacga tagagtagac aacttcgaaa ttgtccttga
     2461 gccttctgaa ggtcagctcg ctcaactatc tcttctgcgt gaatatgtgc atagaacaac
     2521 gggggccgcc ttctcaaatg acctcttcgt gttcacaggg gacggggcaa agggggtaaa
     2581 tataagccag gaggcaatag gtgttcatgc ggaagtcctt aacgtaagct ttggagaagc
     2641 actggaagtc atgttccatg aacttgcgca caatgcgtac atgaaccaca gcagcgagtt
     2701 tatgtcacaa atgatgtcac tcagtatgcg ttcacaagag aatgttatgg agctactcga
     2761 aaagagtgag cgacgagaaa aacttacgcc agaagagcgt tttgtactta ccgtacgcaa
     2821 ggagtggagt gcgttacaga caggtaagaa agtaaaacgt cgtaaataga gccttttcta
     2881 aagactcttc ccgtcgaact tcgtgatagt aagcgtctcc ggatcaacgt catcaaggca
     2941 gcgcacgtta agcgctatca tctcgcttcc atccattcct ttaccgcgcc catagctctg
     3001 tacgccgcag gtagggcaga agaggtggct aatgtgtttc ttgttgaata gatactcggt
     3061 aaggtcggac tcctttccgg caattatgcg aaaggccgcg ggggcgacga acgtaagaag
     3121 aaaccctttc tttccgcagt aggagcagtt acagctcatc gcaccatcaa gtgcaacatc
     3181 cgcttcatag cggagggcgc cgcagtggca accgccagtg taggttttca tgcgagcatt
     3241 ctatcatttt ataaaagaca agcccgaatg gaacactcca ctcgggcctg ggttcgacgc
     3301 aatcgccaga tcacatcttc ttgagttctt cggttagcgc gcgcagcacc ttccgatgct
     3361 ttggtttact gagaagttcg aggagcgcga cgtcttcaag tggtaccact gcggccacat
     3421 cttggccgta gcgggacaaa atacgacgct tctttttcgt tctcgacgca tccaacgcat
     3481 cggcgaagcc tgcacgtgct tccgatgttg aattgcgggg aagggattta aggaggcctt
     3541 cccagcctgg aacaggatca atcctgttta tggtttttcc aggatttttc ttcgctttat
     3601 gtgccatcta attacctcca caatggccta cggaagtatt atcgcactaa aaatgtataa
     3661 agtaaagtct tacgaaagaa gggagtctac gacctctttc acgacgccgc cgtctgcctg
     3721 acccttaagc tctttcatca cggcaccggt gagctgtcct gcctttgatt tatccgcacc
     3781 catttcagca atctttgctt ttaccactgc ttcaatttct tcgcggggca tctgggcagg
     3841 caatagtgcc tcgataacgg caagttcaag cttttctggc tctgagagct ctgaacgtcc
     3901 tgccgtatca aactgctgta tggagtcttt gcgctggttt gcggcacgct taaggactgc
     3961 gagggcctca tcatctgtga gaaactcgtc cggcttacgc ttcttcgcga ccacttcgtt
     4021 ggtcatcata gtaaccaagg agcgataggt acggagcttt acttcgtcgc gtgccttcat
     4081 tgcgtcctta atacctgcct ttattgtttc gtgtagtgcc atatgtgggc aaggatagca
     4141 tagacattgc tatactaaag gtatggacat gcgcccctgg catgcgcttc ccgccacaga
     4201 agcgctcact gcatttaata taagtgtcga gaaaggtctc gatgctgaag aagcggcacg
     4261 ccgactaagt accggcgcga ataccattcg tgccggagca agcgatacgc ttctgaaaca
     4321 gttctggcgc cagataaaaa gcccgatagc cattgtgctc ttgctggcag cactcgctac
     4381 ggtgcttatc gcgcattacg ccgatgcgtt catcatcacg cttgcgctca tggtgaatgt
     4441 cgccattggt atcttccagg aaggcaaggc gggagatgcc ttcaaagcgc ttgctaaaga
     4501 agaggcgccc cgagcccaag tcatccgtaa tggcctgaag cgtgaaatcc ctgcgtctga
     4561 agcggtaccg ggagacattg tggtgctcac cgcaggcgcc aaagcacccg ctgacatacg
     4621 tctcatagag acgcacggcc tctccatgaa cgaggcagcg ctctccggag agtggctccc
     4681 gtcagaaaag aatagtgacg ctgtggggat ggatgctcct ctcgttgagc gcaccggtat
     4741 ggcgtacgcg ggaacgcttg tggtgacggg cgctgctacc ggcatcatcg ttgcaacggg
     4801 gaacgatacc gagctcggga agatcgcaag cgaactcggg aatcgccagg gagagacgcc
     4861 gcttcaaaaa gatattaaag gtattgcgcg cctgcttctc attatcgtgg cactggtgat
     4921 tgcgctcgtg gtcgtgctcg cgctcactca cggcataacg tttggggaga cattactcat
     4981 tgctatcgcc attgccgtcg ctgctattcc ggaaggcctt ccggcagcag ttacggtggt
     5041 gcttgcgtta ggaatggagc gtattttaaa gcagggaggt cttgtaaaga gcttacttgc
     5101 ggcagaaacc cttggtgcaa cgagtatcat tctgaccgac aaaacgggaa ccctgaccga
     5161 aggacgcatg aaagcggcgg gcttcgtgac gctttcgggc actacggaag aagcagacgg
     5221 agaaaacgca cgcttgatgc tccgcgctgc cgtgctcgcg tctgatggct acatagaaga
     5281 agtagatgag tcgacgaccc cgggcgacga gatcatcgca cgcgggcgtc ccatggagca
     5341 ggcgatactg ctcgctggac ttgaagcagg gttctctgag cgggcgcttc gcactgaatt
     5401 cccgcgggtt gatgagcttc acttcacttc tgcccgcagg ttcggcggca tgctcgttgc
     5461 agaacatggt aagcatgtga cgtacctcac cggagtccct gaactcttcc ttgatcacgc
     5521 gcgccgttcg ctcggcgcac gcggtggtga acgtgcgttt acaaaagaaa acgctgcgta
     5581 ctttgaagaa gcactcttgc gtgctgcgtg tgaaggga
//
LOCUS       DAORIN010000034         5560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2772_length_5560_cov_5.098638, whole genome shotgun sequence.
ACCESSION   DAORIN010000034 DAORIN010000000
VERSION     DAORIN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2772_length_5560_cov_5.098638"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PK109_01970"
     CDS             complement(<1..1299)
                     /locus_tag="PK109_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRH24335.1"
                     /translation="MAGTYVHLHTHSHYSFLQALPKVDELVDKAKKEGMTAIALTDAG
                     NLHGTIDFYKYATKKGVKPIVGVDAYIAPGSRHSRDTGEKHSRLVLLAENMTGYENLL
                     KLVTASFTEGMLERPRMDRELLKQHAAGIIALVPSFAGASSQAIAAGDTDGARAVIEE
                     YQNIFGKENVYLEITHHPKVAGHEKRMEEVRALSKASGAPLVAQHDVYYLSPDDSEAT
                     AVLRRIGQGGEGRNEEEDFSFVSEAWMRKHFSSEEIEESSRIAERCSITFDLGKWVFP
                     ALPVSEGYKDHDEELRAKTYAGITARGLEETEEVTTRIEYELSVIHQKGFATYFMVVS
                     DLLNFAHKNNILTTIRGSVGGSLVTYLIGITNVNPLEYKLPFERFLNPERPSAPDIDM
                     DYADNRRDEMIAYAKEKYGEDRVAQIGTFGTMLARAAVRDV"
     gene            1322..1906
                     /locus_tag="PK109_01975"
     CDS             1322..1906
                     /locus_tag="PK109_01975"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRH24336.1"
                     /translation="MTFVLGVDEAGRGPLAGPVAVGVVLAPEGFDFLTAFPGLNDSKK
                     LSEKKREALFTKLVECSAAGELQVAVVLKSAKEIDGRGIAVVIRNAVAEAVQKLLPDP
                     TKGKVWLDGSLKAPDGYVQETVIGGDGLVPAIMLASIAAKVSRDRHMVRLATKYPGYD
                     FEKHKGYGTASHIKVIREIGLSDLHRKSFCKNFA"
     gene            1953..2180
                     /locus_tag="PK109_01980"
     CDS             1953..2180
                     /locus_tag="PK109_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24337.1"
                     /translation="MRKKGLPKKERLARLELAENAFLTCATCPYAGRLVIPKKVPILT
                     VRCKKSKALVPFKEANACLVRPPRGEHPDEE"
     gene            2252..2425
                     /gene="rpmF"
                     /locus_tag="PK109_01985"
                     /pseudo
     CDS             2252..2425
                     /gene="rpmF"
                     /locus_tag="PK109_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
     gene            2717..3634
                     /gene="nusB"
                     /locus_tag="PK109_01990"
     CDS             2717..3634
                     /gene="nusB"
                     /locus_tag="PK109_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRH24338.1"
                     /translation="MANRHLARSVVLQTLFEWDTAKTPSDQVTGVLERNAAEFGGSDT
                     DKPFMEQLLQGVVAKCPDLDLIIVKAAPDWPLERIAPIDRNILRIGLYELLFSDRTQV
                     PPKVAINEAIELAKIFGGDSSGRFINGVLGAVYKEMGEPGKDEQGKKPVVRKKYEDIP
                     VQQLGGAVVYAYHEGQYYLALVHDVFGHWTLSKGKIGDREDIKNETVEEGTKREVKEE
                     IGLDVVIEEKIGENEYVASHPEYGKYRKHVTYFLARSPYDALHLKKSGGLDDAKWFRL
                     PDILGLNFYDDMLPIVTAAVQKLLDKSSQ"
     gene            3667..4350
                     /gene="rnc"
                     /locus_tag="PK109_01995"
     CDS             3667..4350
                     /gene="rnc"
                     /locus_tag="PK109_01995"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRH24339.1"
                     /translation="MDFLENAQKLGLSFKNLDLFEEAFTHRSYLNEHKSAKAHNERLE
                     FLGDAVLELSATHFLFHKYPDKSEGDLTAYRAALVNTYSLAEVAEALGVNDMLLLSKG
                     EKRDMGRARQIILANAFEALVGALYLDQGYEAADAFLTKHLYPKLESIIANRSYQDAK
                     SRFQEVAQDKKGITPSYKTIREDGPDHDREFTVGVFIGEKEIARGLGKSKQEAEQKAA
                     QAGLDKTGW"
     gene            4656..5558
                     /locus_tag="PK109_02000"
     CDS             4656..5558
                     /locus_tag="PK109_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRH24340.1"
                     /translation="MPRTPGEQRPPVSAESAESLIASRALTFALASIKERFETGPEED
                     RLTFHNQEHTEGVVRRAEAIAKAMHLPVREQLLTKIAAAFHDVVQKWEPDAKESGAVM
                     RKRMVRVNEQESADEAVAWMRASGESFTQEEEALVRAAITATVPTWDPENGTVKQTDL
                     ESDVHPITRAVALADLGAAGMETAVSNKEGIALFAEENIDFTNAVRDSKGDVTRIPEA
                     DQEQYQRRCVRYLQQQVTFIEGRQKRLEVELEGLAEPARSNVAALFSAFEASLEQAKV
                     DAQTFDILPFEAIARRLVPDAFAE"
BASE COUNT         1341 a         1420 c         1547 g         1252 t
ORIGIN      
        1 cacgtcgcgc actgccgcac gggcgagcat ggtgccgaag gtgcctatct gcgccacgcg
       61 gtcttctcca tacttttcct tagcgtacgc aatcatctcg tcgcgtcgat tatcggcata
      121 gtccatgtcg atatcgggcg ctgagggacg ctccgggttt aggaagcgtt cgaagggaag
      181 tttgtactca agtgggttca cgttcgtgat accgatgagg taggtgacta aggagccgcc
      241 cacggaacca cgaatagtcg tgaggatgtt gttcttatgc gcaaagttca gaaggtcaga
      301 caccaccata aagtaggtag cgaatccttt ttgatgaatg acactgagct cgtactcaat
      361 gcgtgtggtc acttcttcag tctcttcaag cccccgtgcc gtgataccgg cataggtctt
      421 ggcacgaagt tcttcgtcgt gatccttgta cccttcggaa accggaagcg cagggaagac
      481 ccactttcct aaatcaaagg taatggagca gcgttcagca atacggctac tctcttcaat
      541 ttcttcagaa gagaagtgct tacgcatcca cgcctctgaa acaaatgaga aatcttcttc
      601 ttcgttcctg ccttcgcccc cctggccaat gcggcggagc actgcggtgg cttcgctgtc
      661 gtccggcgag aggtagtaga cgtcgtgctg cgccactaac ggtgcgccgc ttgctttgga
      721 gagtgcgcgt acttcttcca tacgtttctc gtgaccggcg acttttgggt gatgcgttat
      781 ctctaagtac acattctctt taccgaaaat attctgatac tcctcaatga ccgcccgggc
      841 gccatcagtg tcacctgcag ctattgcttg cgaggaagcg cccgcaaagg agggaacgag
      901 cgcgatgatg cctgcggcgt gctgctttaa aagttcccgg tccatacgag gacgctcaag
      961 catgccttcg gtaaacgatg cagtgacaag tttcaacaga ttctcatagc ctgtcatgtt
     1021 ctcagcgagc agtacgaggc gtgagtgctt ttctccggtg tcacgactgt ggcgcgagcc
     1081 gggggcaata taggcgtcga cgccgacaat aggcttcacg cctttcttgg ttgcgtactt
     1141 atagaagtcg atagtgccgt ggaggttccc agcgtccgtt aaggcaatgg cggtcatgcc
     1201 ttctttcttt gccttgtcca cgagctcatc taccttcggc agcgcctgga gaaaggagta
     1261 gtggctatgc gtatggagat ggacgtaggt tcctgccata tagggttagt atactttcca
     1321 catgactttc gtgcttggcg tggatgaagc aggacgcggc cccttagcag ggcccgttgc
     1381 tgttggcgtc gtcttggctc ccgaggggtt tgatttcctt acggcgttcc cgggcctcaa
     1441 cgactccaag aaactctccg aaaagaagcg tgaagcgctc tttaccaaac ttgtggagtg
     1501 ctcagctgca ggcgagcttc aggtagcggt ggtgttgaaa agcgcaaaag aaattgatgg
     1561 cagaggtatc gcggtcgtca tacgaaatgc tgtcgctgaa gcggtacaga aattgttacc
     1621 cgaccctaca aaagggaagg tgtggctcga tggttctttg aaggcgcccg atggatacgt
     1681 tcaagaaacg gtcatcgggg gagacggact tgttcccgcc atcatgctcg cctctattgc
     1741 cgcaaaggtt tcgagagacc gtcacatggt gcggttagcg accaaatacc caggatacga
     1801 ttttgaaaag cataaagggt acgggacggc gtcgcatata aaggtcattc gtgagatagg
     1861 gctctctgat cttcatagga agagtttctg taagaacttt gcataagagt gggtgaggag
     1921 tagagtgcgc tagttcgaaa cggggaagca tcatgcgaaa aaaaggctta cccaaaaagg
     1981 aacggctggc acgcctcgag cttgccgaga acgccttcct tacgtgtgct acctgtccgt
     2041 acgcaggccg gctggttatt cctaaaaagg tgcccatcct caccgtacgg tgcaagaaaa
     2101 gtaaggcatt agtgcctttt aaggaggcaa atgcctgcct cgtccgtccc ccgcgtgggg
     2161 agcacccgga tgaggaatag agtgccgctt ggacccattg gggacccggg cggcatttct
     2221 ttttataagc ccctatggta aggtagccct catgtcagtt cgaatgcgcc atacctcggg
     2281 tcacacccgc aaccgccgaa gccaccacgc tttgaagaat aaggttatcg tcaccgataa
     2341 ggagtcgggt gctctccgcc tcccacaccg ccttgatgag accaccggca tgtaccgcgg
     2401 taagcagatt gttgccaaga aggaagttaa ggagaaggca aaggatcata agaagcgcgt
     2461 tgaggcgcat aagcatgacg atgttgccgg cgacaagcag ccagtacacg ttgaagcaaa
     2521 agaggcagta aagaaggaag gggtccttgg tcgcatgacc aaaggcaagg caaaggcccg
     2581 ctccggattc ggcggcggcg cataagccac ccctcgttct cgtgaaaaac ccgcgacctc
     2641 tgtcgcgggt ttttctttgt tatgcacagg aaggggataa gagagcgcca cccatttgtt
     2701 acgctagtta gtagatatgg ctaatcggca tcttgcccgc tccgtcgtgc tccaaaccct
     2761 cttcgagtgg gatacggcaa agacgccgtc tgatcaagtg acgggcgtgt tggaacgtaa
     2821 cgctgccgag ttcggaggaa gtgatactga caaaccgttc atggaacaac tccttcaagg
     2881 agtggtggct aagtgcccag atctcgatct cattattgtt aaagcagcgc ccgactggcc
     2941 gcttgaacgc atcgcgccca ttgaccgcaa tattctgcgc atcggcctct acgaactcct
     3001 cttctctgat cgtactcagg tgccgccaaa ggtagcaata aatgaagcga tagaacttgc
     3061 gaagattttc ggtggtgact cttctggtcg ctttataaac ggggtactcg gtgccgtgta
     3121 taaggaaatg ggtgagcccg gcaaagacga gcaaggaaag aagccagtgg tacggaagaa
     3181 gtacgaagat attcccgttc agcagttggg aggtgccgtc gtctacgcgt accacgaagg
     3241 tcagtactat ctcgcactcg tgcacgatgt gtttgggcac tggactctct ccaaaggaaa
     3301 gattggtgac cgcgaagata taaagaacga gacggtagag gagggtacta agcgcgaagt
     3361 aaaagaggag attggacttg atgtggttat cgaagaaaag atcggtgaga acgagtacgt
     3421 tgcctcgcac ccggaatacg gcaagtacag aaaacacgtc acctacttcc tcgcccgctc
     3481 accctatgac gcccttcacc ttaagaagag cgggggactc gatgacgcga agtggttccg
     3541 cctcccggac atcctcggtc tcaatttcta tgacgatatg ctccctattg tgaccgctgc
     3601 cgtgcagaag cttctggata aatcctccca gtagcctttt tacctctact agggtacttt
     3661 cccctcatgg actttctcga gaacgcccaa aaactaggac tttccttcaa gaacctcgac
     3721 ctcttcgagg aggcgtttac gcatcgctca tacctgaacg aacataagag tgcaaaggcg
     3781 cataacgagc ggttggagtt cttaggtgac gcagtactcg agctttctgc cacgcacttc
     3841 ctttttcaca aatatcctga caagtcagaa ggcgatctca cggcataccg cgccgcactc
     3901 gtgaatacct actcacttgc tgaagttgcc gaagcgcttg gggtgaatga catgctcctt
     3961 ctttcaaaag gtgagaagcg agatatggga cgcgcgcgtc agatcattct tgccaatgcg
     4021 ttcgaggccc ttgttggtgc gctctatctt gaccaaggct atgaggcggc agatgcgttt
     4081 cttactaagc acctctaccc aaagcttgaa agcattattg caaaccgttc ctaccaggat
     4141 gcgaagagtc gtttccagga agtagcgcag gacaagaagg gaattacgcc gtcctacaaa
     4201 accatacgcg aagacgggcc tgaccatgac cgtgaattta cggtaggcgt tttcatagga
     4261 gagaaagaaa ttgcacgcgg cctagggaag tcaaaacaag aagcagagca gaaagcggca
     4321 caggcggggc tggacaaaac aggctggtaa ccggtaaata cgtacagtga gtcctgatta
     4381 gcaggattca cgtgagggag ggtgccatgt tccatcggct ctctagctat ttcaacaacc
     4441 ttggtgacga catcgttcac ggtatcggat gcgtctacgt atgcatcgtt atagccggat
     4501 gtgtcgctat cgcgctcgta tgcaatgtgc cttttcagat ggctacgctc gttcgaaacc
     4561 gttcgtaacg gttcgatcaa gtactgaact gaaaagcatg aacgccgctt ccgtgggaag
     4621 cggcgtttct gatatgaatc ctgtagtatt gaggcatgcc acgcacccct ggagaacagc
     4681 gaccgccggt atctgccgaa tcagctgaat cgctcatcgc ttcccgtgcc ctcacgttcg
     4741 cgctcgcttc cattaaggaa cgttttgaga caggtcccga ggaagaccga ctcacctttc
     4801 ataatcagga acatactgaa ggggtggttc gtcgcgctga agcgatagcc aaggctatgc
     4861 accttccggt gcgcgaacag ctcctcacca agattgcggc tgcctttcat gatgtggtgc
     4921 aaaagtggga gcccgacgcg aaagagagtg gtgcggtgat gcgtaaacgt atggttcggg
     4981 taaacgaaca ggaaagtgct gacgaagcgg tggcatggat gcgtgcctca ggtgaatcat
     5041 ttacccaaga agaagaggca ctcgtgcgcg ctgcaattac cgccaccgtt cctacctggg
     5101 acccggaaaa cgggacggta aagcaaacag atctagaatc tgacgtgcat cccattacgc
     5161 gcgctgtggc gctcgctgac cttggggcag ccggcatgga gacggcagta tctaacaaag
     5221 aaggcatcgc tctttttgcc gaagagaaca ttgatttcac aaatgcagta cgggactcca
     5281 aaggcgatgt aactcgtata ccagaagctg atcaggaaca gtaccagcgt cgctgcgtac
     5341 gatacctcca gcaacaagtc accttcatag aggggcgtca gaagcggctc gaggttgaac
     5401 ttgagggtct tgcggagccg gcacgcagta acgtcgcggc actcttttct gccttcgaag
     5461 catctctgga gcaagcgaaa gtggacgcgc agaccttcga cattctcccg tttgaggcta
     5521 ttgcacgccg actcgtcccg gatgcgtttg cggagtaggg
//
LOCUS       DAORIN010000035         5500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2823_length_5500_cov_4.463177, whole genome shotgun sequence.
ACCESSION   DAORIN010000035 DAORIN010000000
VERSION     DAORIN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2823_length_5500_cov_4.463177"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PK109_02005"
     CDS             complement(<1..1043)
                     /locus_tag="PK109_02005"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRH24341.1"
                     /translation="MASDGHKYIFVIGGVMSGVGKGIAVSSIGLLLKQRGYPVNLVKI
                     DPYLNVDAGTMNPTEHGEVFVLRSGLETDQDMGNYERFLDRDLDNDDYMTSGMVYRTV
                     IDNERALKYGGKCVEAIPHVRDEILRRIESAAEHNGSKISVIEIGGTVGDFQNALFID
                     AARVMKMKRPNDVIFVMVSYLPVPGTLGEMKTKPTQSAIRELNSYGVQPDFIIARSTA
                     AIDEKRKEKLATSCNLLPERVISAPDVASIYEVPLNFEKDEFGDHLLDTLGLSEKKKP
                     DLKDWQKFVDGLRITEPEVKIGIVGKYFDTGDYILSDAYLSVIEGLKFSGAKIGAKPK
                     IDWINAKEFQEGKG"
     gene            complement(1046..1972)
                     /locus_tag="PK109_02010"
     CDS             complement(1046..1972)
                     /locus_tag="PK109_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRH24342.1"
                     /translation="MSTMMIRRVFVNGAKNFARSGSVTFATVLIMTVTLAIIGLLIFL
                     SAVLGHTLESIKDKVDVNVYFVTTAAEGDIIAFQNELAELPQIERVSYTTRDEALIAF
                     KARHSTDELTLQALEELGDNPLGASLSIKATDPGEYESIVNYVSEHAPTDAAGSSLID
                     RVNYFQNKIVIDRLTGAIHATEQAGAAIVILFALASAVIAFATVRLAIYTARDEIAVM
                     RLMGASNGYIRGPFIVAGVIAGILAAIIVLVLLYPVSWYVGGALAGWLGGFNLFTYYV
                     EHFALFFGILVGSGFLLGGLASWFAVRKYLRM"
     gene            complement(1974..2654)
                     /gene="ftsE"
                     /locus_tag="PK109_02015"
     CDS             complement(1974..2654)
                     /gene="ftsE"
                     /locus_tag="PK109_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRH24343.1"
                     /translation="MIYFDKVTKQYRDGTPALEEVTLSVSPKEFISIVGHSGAGKTTL
                     LKMLLAEERPSTGSVFFEDVDIHDLPKNALHHYRRKIGTVFQDFRLIPTKTAYENIAF
                     AMEAVGRRDDEIESDVPYILDLVDLGDKANHFPHQLSGGEQQRVAIGRAIINQPDVIV
                     ADEPTGNLDPVNTYDVIEILKKINELGTTVIITTHNKGVVDAMNKRVITMDKGRVVRD
                     DPHGHYIL"
     gene            complement(2665..3537)
                     /locus_tag="PK109_02020"
     CDS             complement(2665..3537)
                     /locus_tag="PK109_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRH24344.1"
                     /translation="MTEDNPRLQELEAAMSSADFWADKDAAQAIVREYQALKEGGSGD
                     PLDAGDATIAILAGAGGDDAEDFAGMLRRMYEGCAAKKGWGARELDATENNHGGYRSV
                     LLEISGRGAYGRLRTESGVHRLVRISPFNAQDKRQTSFALVEVLPKLAATLKVDLNTE
                     DLDITFARAGGAGGQNVNKRETAVRIVHTPTGISAHVSSERSQQANREKAMELVKAKL
                     YKFQEEQRLAEAEGRSIAGTVKNEWGSQMRSYVLHPYKMVKDHDSGHESGNPDAVLSG
                     ELDDFIDAAHAPGV"
     gene            complement(3578..3653)
                     /locus_tag="PK109_02025"
     tRNA            complement(3578..3653)
                     /locus_tag="PK109_02025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3618..3620),aa:Thr,seq:cgt)
     gene            4531..>5500
                     /locus_tag="PK109_02030"
     CDS             4531..>5500
                     /locus_tag="PK109_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HRH24345.1"
                     /translation="MCGIIGYVGPKNATPILIEGLKALEYRGYDSAGIFVAGAGAIKR
                     SGSVSALADAIPQEFVGHAGIGHTRWATHGAPTETNAHPHSDPSGTVYIIHNGIIENY
                     ADIKAMLVKDGVVFKSETDTEVLAALIGKKYAPGVPLEIAVSEALKEVRGTYGLAVAS
                     TREPAKLVAARLSSPVMLGIGKDEYYIASDASAILKHTRDVIYLDDGEVATLTPDGYE
                     VMTLTRTPREKAVETLEWDVEAAQKKGYDHFMLKEMMEIPETIENTLRGRLLLEEGDA
                     KLGGLRDVLPRLRAAKHITITACGSAFYAGKVGELLIEEFAGIPVEV"
BASE COUNT         1273 a         1474 c         1407 g         1346 t
ORIGIN      
        1 cccttaccct cctggaactc ctttgcgttt atccaatcaa tctttggctt agcaccaatc
       61 ttggcaccgg agaacttaag accttcaata acggaaagat acgcgtcaga aagaatgtag
      121 tcgcccgtat caaaatactt gcccacgata ccaatcttca cctcaggctc agtgatgcga
      181 agtccatcga caaacttctg ccagtcctta agatcaggct tcttcttttc tgagaggcca
      241 agcgtgtcta ggagatgatc gccgaattcg tctttctcaa agttaagtgg tacctcgtat
      301 atcgacgcta cgtcaggagc ggagatgacg cgctccggga gaaggttgca ggaagtcgca
      361 agcttttctt tacgcttctc atcgatggct gccgtagagc gcgcgatgat gaagtcaggc
      421 tggacgccat aggaattcaa ttcgcgtatc gcgctctgcg tgggcttcgt cttcatctcg
      481 cccaaggtgc ccgggactgg gagataggag accatcacga agatgacgtc gttcggacgc
      541 ttcattttca taacgcgcgc cgcatcaatg aaaagcgcgt tctggaagtc ccctaccgtg
      601 cctcctatct caatcactga aatctttgag ccgttatgtt ctgcagccga ctcgatgcga
      661 cgaagaattt catcgcgtac gtgcgggatg gcttcaacgc acttaccacc gtacttgagt
      721 gcgcgctcgt tatcgatgac ggtgcggtac accatgccgg aggtcatgta gtcatcgtta
      781 tcaagatcgc gatcaaggaa gcgctcgtag ttacccatgt cctgatcggt ctcaagccca
      841 gaacgcagta cgaatacttc gccgtgctcg gtggggttca tcgtgcccgc gtccacgttc
      901 aagtacggat caatcttcac caggttcacc ggatacccgc gctgcttgag gagaaggcca
      961 atagaagaaa cggcgatgcc ctttccgacg ccgctcatta ctccgcctat gacgaatatg
     1021 tatttgtgtc cgtcggaagc catacttaca ttcgtagata cttacgcact gcaaaccagc
     1081 tggcaaggcc accgaggagg aacccagagc cgacgagaat accgaagaag agcgcaaagt
     1141 gctccacata ataggtaaag aggttgaacc cgccgagcca gccagcaagc gccccaccca
     1201 cgtaccagga cacgggatat aggagtacca gcacaatgat ggctgcgagg atgcctgcta
     1261 taacgcccgc tacgataaat ggtccgcgga tatagccgtt agaggctccc atgagacgca
     1321 tgacggcgat ctcatcgcgt gcggtgtaga tagcaaggcg caccgtagca aacgcaatca
     1381 cggctgaagc gagcgcaaac aggatgacga tagccgcccc tgcctgttct gtcgcgtgta
     1441 ttgcaccggt gaggcgatca atcactatct tgttctggaa gtagttcaca cgatcaataa
     1501 gggaggagcc agcagcgtca gtaggggcat gctctgagac atagttcacg atgctttcgt
     1561 actcacccgg atccgttgcc tttatggaaa gagaagcgcc gagcgggttg tctcccagct
     1621 cttcgagcgc ctggagggtg agctcatcgg tggagtgtcg cgctttgaat gcaatgagtg
     1681 cttcgtcgcg ggtggtgtag gaaacgcgct ctatctgggg aagttccgcg agttcgttct
     1741 ggaaagcaat gatgtcccct tctgcggcgg tagtcacaaa gtagacgttc acatccacct
     1801 tgtccttaat tgactcaagc gtgtgcccga ggacagcaga aaggaaaatg agtagaccaa
     1861 tgatagcgag cgtcaccgtc atgatgagca cggtagcaaa cgtcactgag ccactacgcg
     1921 caaagttctt cgcgccattc acaaacacac gtcgtatcat catggtactc atatcagaga
     1981 atgtaatggc cgtgcgggtc gtcgcgcacc acgcgtcctt tgtccatggt gatgacgcgc
     2041 ttattcatcg catccaccac tcctttattg tgcgtggtga tgataacggt agtaccgagt
     2101 tcgttaatct ttttgagaat ctcgatgacg tcataggtgt tcaccggatc aaggttcccg
     2161 gtaggttcgt cagcgacaat aacgtcgggc tgattgatga tggcgcgacc aatagcaacg
     2221 cgctgctgct cgccgcccga gagctggtgc gggaagtgat tcgctttatc cccaagatca
     2281 acgaggtcga ggatgtacgg gacatcagac tcaatctcat cgtcccggcg ccctaccgct
     2341 tccatagcaa acgcgatgtt ttcgtacgcc gtcttagtcg ggattaggcg gaagtcctgg
     2401 aacaccgtac caatcttgcg gcggtagtgg tggagcgcat tttttgggag gtcgtgaatg
     2461 tccacatctt caaaaaagac gcttccggta gacggacgct cttccgcaag cagcatttta
     2521 aggagtgtgg tcttacctgc gcccgagtga ccgacgatag aaatgaattc tttaggagaa
     2581 accgacagag tgacttcttc aagagcggga gtcccgtctc gatactgttt cgtcactttg
     2641 tcgaaataaa tcatgaccgg agcattatac cccaggtgcg tgcgcagcgt ctatgaaatc
     2701 atcgagttca ccacttaata ccgcgtccgg gttcccggac tcgtgtcctg aatcgtgatc
     2761 tttcaccatc ttgtacggat gcaggacata ggagcgcatc tgactgcccc actcgttctt
     2821 aaccgtgcct gcgatagagc ggccttctgc ctccgcaagg cgctgttcct cttgaaactt
     2881 atacagtttc gccttcacga gttccatcgc tttctcacgg ttcgcctgct ggctacgctc
     2941 gcttgagacg tgggcactta tccccgttgg agtgtgcacg atgcgtactg cggtctcccg
     3001 cttattcacg ttctggccgc ccgccccacc ggcgcgcgca aacgtaatat caagatcctc
     3061 tgtattcaaa tccaccttta aggtagcggc gagcttaggc agcacttcaa cgagcgcgaa
     3121 actggtttgc cgtttgtcct gcgcgttaaa cggagaaatt cgtacgaggc ggtggacgcc
     3181 agactcggtg cgaagtcttc cgtacgcgcc gcgcccgctt atctcaagca atacgcttcg
     3241 gtatcctccg tgattgtttt cggtagcatc taactcacgc gcaccccacc ccttcttggc
     3301 agcacacccc tcatacatac gacgcagcat gccggcgaaa tcttcggcgt cgtctcctcc
     3361 ggcgcctgcg agtatggcaa tagtcgcgtc acccgcatca agcggatcac cggaccctcc
     3421 ttccttgagt gcctgatatt cgcgcacaat agcctgcgca gcatctttgt ctgcccaaaa
     3481 gtctgcagaa gacattgccg cttctagttc ctggaggcgc ggattgtctt cggtcataac
     3541 aacgagtata gcaaaaaccg ccgaagcggt ttttccttgg agccacctcc cgggattgaa
     3601 ccggggacct cttcgttacg agtgaagtgc tctaccaact gagctaaggt ggcgattaga
     3661 gggaagatat cacgtttttt gataattaaa aactatagcg cgcgcatctg ctgcgcgttt
     3721 acttttaggt cgatttgctc gaccttttcc tctatttaaa tttccgaacg ttggcgttaa
     3781 agcgtcacag ttagggcaca aaagtctgag attatcttcc tggttatttt gccatcttcc
     3841 gtcgatatgg tctgcgatta gcggcactat gccagtctga gggttcactt ctgaccattc
     3901 acataggcaa cacttgtttg agaatttttg tcttaagtaa cttttgatcg gatctttcac
     3961 tacccccata gtgctcagtc ctacctcttt accctctttc catttttgta tgaaaacaac
     4021 atattgatac tcaccctgac atttattact acagtattta tacatgggtc tctttggctc
     4081 tttaccacac gcaagacaag gagttctccg cttctttgga ttcattcact acattataga
     4141 aaatcccctt tcggggattt ccactcaact tacacaattt ggagccgatg gtcgggattg
     4201 aaccgacgac ccccaccttc gcttacacca ctccttcgtg catacctgga gcgggctaga
     4261 ctgtatcttt atccgcttgg gatacccttg tcagtcgttc gggcgattac tcgccacggt
     4321 cttgccttgt atgaggtatt aaccgttatt cgggattcac cagaagatta ctcttcaagt
     4381 gggcagggtc ttgcgaccta ccatggtggt gctctaccaa ctgagctaca tcggcttttt
     4441 gttctatacg aacactgccg atagtacgtt atttatgcca ttctttcaat ttcccttccc
     4501 gataccgggt aaagggtata cccttcttag atgtgcggca tcatcggata cgtcggaccg
     4561 aaaaacgcca ctcctattct tatcgagggg ctcaaagctc ttgagtaccg cggctacgac
     4621 tctgcgggaa tttttgtcgc tggcgcaggc gcaataaagc gttcgggaag tgtgagtgcg
     4681 ctcgctgatg ccattcctca ggaatttgtt ggacacgctg gtatcggcca cactcgctgg
     4741 gcaacccatg gtgctcctac ggaaacgaac gcgcacccgc acagcgatcc ttcgggaacc
     4801 gtctatatca tccacaacgg catcattgaa aactatgcgg atattaaagc gatgctcgtg
     4861 aaagacggcg tagtgttcaa gtcagaaacc gatacggaag tgcttgcggc attaattgga
     4921 aagaaatatg cgcccggcgt accgcttgag attgcggtct ctgaagcctt aaaagaagtg
     4981 cgcggcacct atgggcttgc ggtagcgtct acgcgcgaac ccgcaaagct cgtggcagca
     5041 cgactctcaa gtcctgtgat gctcggtatc ggtaaagacg agtactacat cgcttcagac
     5101 gcaagcgcca tcttaaagca cactcgtgat gttatttatc ttgatgatgg agaggtagca
     5161 acgcttacgc cagacgggta cgaagtcatg acccttaccc ggacgccgcg cgagaaagca
     5221 gtagagacgt tggagtggga tgtagaggct gcgcagaaaa aaggatacga ccatttcatg
     5281 ctgaaagaga tgatggaaat tccggagacc atcgagaaca cgcttcgtgg ccggttactc
     5341 cttgaagagg gtgacgcaaa gctcggcgga ctgcgagatg tgttgccacg actccgtgct
     5401 gcaaagcaca tcaccattac cgcctgcggg agtgcgttct atgctgggaa ggtcggtgaa
     5461 cttctcattg aagagttcgc cggtattccg gtagaggtgg
//
LOCUS       DAORIN010000036         5460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2867_length_5460_cov_5.544681, whole genome shotgun sequence.
ACCESSION   DAORIN010000036 DAORIN010000000
VERSION     DAORIN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2867_length_5460_cov_5.544681"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK109_02035"
     CDS             <1..452
                     /locus_tag="PK109_02035"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24346.1"
                     /translation="SGGFTVKDDAIFYLEERLCASYAALPKADDAIDAMADAELSAFD
                     ATDALLSRMARLAPFGMGNSKPIFAFRGVSLSRVSWFGKSEEHLRLHLTTDGFDSLEG
                     IAFYAKRELGSGVKGLEAGGQVNILASLERDQFTKGRPVRLRLVSVS"
     gene            506..844
                     /locus_tag="PK109_02040"
     CDS             506..844
                     /locus_tag="PK109_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24347.1"
                     /translation="MKISLNALKWVDAFLGCILLLELVTVIVVAMFGLDDEWPLSELW
                     NYRIFLSTAGLYLVSRIAYTSRLKDNPDVKAGQGGGNKVLVYGIGIVLLLIILWILKI
                     AIGIGGATSF"
     gene            1244..1666
                     /locus_tag="PK109_02045"
     CDS             1244..1666
                     /locus_tag="PK109_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HRH24348.1"
                     /translation="MLYTLYADGGARGNPGPAGAGAVVFDSIGKRVVEVSDYLGVATN
                     NIAEYEAIIRGLKALQAEFPEGHFKTNEVLIRMDSKLVIEQMKGNYKVKHPNLVPRCL
                     EAKNVIARGFSKVSFEHVPREKNKDADELANRAMDKGR"
     gene            1670..3151
                     /locus_tag="PK109_02050"
     CDS             1670..3151
                     /locus_tag="PK109_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRH24349.1"
                     /translation="MLSRLLITGLIGLALGIAAASLFTVPFAAVAFVLLIGGTFLALW
                     NVKKEAVLLIVSIFFLTAACGMARVLVVPSELPVTYAPLIGQTIEIEGVVVADPDIRE
                     TTQRLTIEVKEDGVRILAVVPTYPRASYGDTIRVTGMLETPEPFDTTGGRVFRYDKFL
                     AKDGIFITMQSREVTIVTPRNGIGERIRGALSDAKVAGLAALAAALPEPHASLAGGLI
                     LGGKQGLGETLLNDFITTGLVHIVVLSGYNVMIVADAVFRVFGFLSQKSAAIAAGVTI
                     LSFVLAAGAGAASVRAGIMATIALYGRATGRTYHAVRALIVAGVLMVLWNPLTLMFDP
                     GFQLSFIATLGLIYGAPITERWFSFVKIAMLKEIIASTVAAQIAVLPLLLYQNGLLSL
                     IALPANVMVLPLVPFAMLASAIAGAAGFLIPAAAPVFAFPAYLLLSYITGVVEIAAHL
                     PYAAVTLPAFPFWVVVVSYALLGWGTVRATRPALENASQQRPS"
     gene            complement(3105..3788)
                     /locus_tag="PK109_02055"
     CDS             complement(3105..3788)
                     /locus_tag="PK109_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HRH24350.1"
                     /translation="MRRREGTVGIHQHITAERDILQTISNPDTMTYTARKFAIPELKG
                     LSQKQLEVHLALYEGYVKHTNLILDTIKELRTADAEKNAFVINELRRRMGFEFDGMRM
                     HEYYFEQLEGGAKEAGKTSPLRDLVEDKYGDWDKFIAHVKEVAGTRGIGWTVVYYDFA
                     ERVLHTVWVSDHELGQLAGLPIILAIDMWEHAFMVDYVPADKKKYVDAFLENLNWDVV
                     EKRFLEQVV"
     gene            3769..4626
                     /locus_tag="PK109_02060"
     CDS             3769..4626
                     /locus_tag="PK109_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRH24351.1"
                     /translation="MPSRRRIALLVVLLVLVALTAAVWWAVLEERPAYLTVSFLDVGQ
                     GDAILIEGPTGIQMLIDGGRDRAVLRELPKRMGPLDRSIDIVVETHPDADHIGGLPSV
                     FERYAVGKFMGPGIGNATNITDALISAIDTEPSLEQVLARRGMRIDLGGGAYADILYP
                     DRDVSMLESNEGSIVMRVVYGDTSFLLGGDAPSIVEDAIALTYGTAIQSDVLKAGHHG
                     SRYSTDDYWLSIIDPTVVIISAGAGNSYGHPHAEVLERVQKSGASLLSTIDEGMIVLE
                     SDGTDVIQK"
     gene            complement(4658..>5460)
                     /locus_tag="PK109_02065"
     CDS             complement(4658..>5460)
                     /locus_tag="PK109_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24352.1"
                     /translation="FIPQERYTFCRRSFYRASPINSEDTALPKPKMITIDSCDFPIVE
                     TEREDYKVLMLPSDVRTLFDEEGAHVVRINSLEIAKRIPDYGGKTSGNYSLLPHQDHL
                     GKDMKARFLALSNLSEGPRESVTVVTLPNTAARILAIEEAYFSDPERRSAIGKNREYD
                     PRFCITEAQYDRCFDHEDGYEEAVAVVAGKRPSAALELAARLGIYGYLMRGPAHADEL
                     MQRIVARTKNSLLIEEWNEPGVVIIDNDRVFHARFGGNTPPLKRNFFD"
BASE COUNT         1181 a         1359 c         1488 g         1432 t
ORIGIN      
        1 cgtcaggcgg gtttacggtc aaagatgacg ccatttttta tttagaagaa cgtctctgtg
       61 cttcgtatgc tgcactcccc aaagctgacg atgctattga tgcgatggcg gacgctgagc
      121 tttcagcgtt tgatgcaacg gatgcacttc tctcacgtat ggcacgcctt gctccgttcg
      181 ggatggggaa cagcaaaccc atatttgcgt ttcggggggt ctcgctctct cgggtctcgt
      241 ggttcgggaa gtcagaagaa catctacgac tccacctcac caccgatggc ttcgattccc
      301 tggaaggtat cgcgttctat gcgaagcggg agttggggag tggggtaaag gggcttgaag
      361 ctgggggtca ggtaaatatt ctggcgtcct tggagcgtga tcagtttacg aaagggcgcc
      421 cggtgcggtt acggctcgtc tcggtttcgt aggttcgaat cctggtccgc caacagtcat
      481 tttataggct agactatata ggcatatgaa aatctccctc aacgccttga aatgggttga
      541 tgcatttctc ggttgtatcc ttctgctcga actcgtcaca gtaatagttg tagcgatgtt
      601 cggccttgat gatgaatggc cgctttctga gctatggaac tatagaatct tcctcagcac
      661 cgctggttta tatcttgtct ctagaatcgc atatacctct cgtttaaagg ataatcctga
      721 cgtgaaagca gggcagggag gaggaaataa agtacttgta tatgggattg gtatcgtact
      781 actcctcata attctatgga ttcttaagat tgctataggt attggagggg ctacaagctt
      841 ctaaaaaagt tccataatcg tcccagacag ccaaagttta gttagattcg gaatcgaatc
      901 ttgacaatat gaccaaaata taggatactc ttttagtact tacttctatc agctagaaga
      961 ggagaacggc ccatgttctg gaaccaattg gctcgcgagc tgaatcccga ggtcgctaag
     1021 catctgctct tcgtgggcag tgcttcaacc atcgggtgtg ctctcggcgc cttcctccag
     1081 ttctgtgtcc tgccggcact tggttactcg gggtaaccct agccaatgcc ctcaatcaag
     1141 gaggcctgct ttcgagcagg cctctgctgt atttaaaagt tcaataatcg tcccagccaa
     1201 tagatgctgg gctctataag actctcagct atactagggc gacatgctct atacccttta
     1261 tgccgatggt ggcgcacgcg gaaacccggg acccgcaggc gcaggtgccg tcgtcttcga
     1321 ctcgataggt aagcgcgtcg tcgaagtgtc tgactatctt ggcgttgcta ccaataacat
     1381 tgctgaatac gaagcgatta ttcgtgggct gaaagcgctt caagcggaat tccctgaagg
     1441 gcactttaag actaacgaag tacttattcg catggactcc aagctcgtta tcgagcagat
     1501 gaaagggaac tataaggtga agcacccaaa cctggtgccg cgctgtcttg aggcaaagaa
     1561 tgttattgcg cgcggattct ctaaggtctc ttttgagcat gtgccgcgcg agaagaataa
     1621 ggacgcggac gagcttgcga atcgggcgat ggacaagggg cgctagatca tgctctcccg
     1681 actcctcatt acggggctga tcggactagc acttggcatt gcggcagcgt ctctttttac
     1741 ggttcccttc gccgcagtcg cgttcgttct acttatcggc ggtacgtttc tagcgttatg
     1801 gaatgttaag aaagaagcag tacttcttat agtgagtatc tttttcctca ctgctgcttg
     1861 tggcatggct cgggtgctgg tagtaccgtc ggaacttccg gtaacttatg cgccgctcat
     1921 cggacagact attgaaattg aaggcgtcgt cgtcgctgat cccgatatcc gtgagaccac
     1981 gcagcgtctc accattgaag tgaaagaaga tggcgtgcga atactggcag tagtgccgac
     2041 gtatccgaga gcatcctatg gtgacacgat acgcgttacg ggcatgctcg agactccaga
     2101 gccgtttgat acaaccggcg ggcgggtatt ccgctacgac aaattccttg ccaaagacgg
     2161 cattttcata acgatgcaga gtagggaggt aacgattgtt acgccaagaa atgggatagg
     2221 ggaaagaata cgtggtgcac tttcagatgc taaggttgcc ggtcttgcag cgcttgcagc
     2281 cgcacttcct gagccccacg cctcacttgc tggcgggctc atcttaggtg gcaagcaagg
     2341 attaggagag acgctcctca atgatttcat tactacggga ctcgtccata tcgttgtcct
     2401 atctggatac aacgtgatga tagtggctga tgcggttttc cgcgtctttg gattcttgtc
     2461 gcaaaagtcg gcagcgattg ctgccggagt gaccatactc tcgttcgtgc ttgcggcggg
     2521 tgccggtgct gcgtcagtac gcgccggcat tatggctacg atagcgctct atggtcgcgc
     2581 gacgggacgc acgtatcacg cagttcgggc gcttatcgtt gcgggtgtgc tcatggtgct
     2641 ctggaaccct cttacgctta tgtttgatcc cggctttcag ctttccttta ttgctaccct
     2701 tggacttatt tatggcgccc ctattactga gcggtggttc tcatttgtga aaatagccat
     2761 gctgaaagaa ataattgcct caaccgtagc ggcacagata gcggtgcttc cgcttctgtt
     2821 gtatcagaat ggactcctct cgcttatcgc gcttcccgct aacgttatgg tgttaccgct
     2881 tgtgccgttt gcgatgcttg catcggcgat agcaggggcc gctggatttc ttattcctgc
     2941 agcagcaccc gtattcgcgt ttcccgcgta cctattactc tcgtatataa cgggagtcgt
     3001 agagatagcc gcgcatcttc cgtatgctgc ggtcactctc ccggcgtttc cgttctgggt
     3061 agtcgtcgtc tcgtacgctc ttctcgggtg gggaacggtg cgtgctacac gacctgctct
     3121 agaaaacgct tctcaacaac gtcccagtta aggttttcaa ggaatgcgtc gacgtacttc
     3181 tttttgtcag cagggacata gtcgaccatg aaggcgtgct cccacatgtc gatagcgaga
     3241 atgataggga ggcctgcaag ctgcccaagc tcatgatcag aaacccacac ggtgtggagg
     3301 acgcgctccg caaagtcgta atacaccacc gtccagccga tgccgcgagt gccggcaact
     3361 tcttttacat gtgcgatgaa cttgtcccag tcaccgtact tgtcttctac cagatcccgt
     3421 aagggagagg tctttcctgc ttctttcgca cccccttcaa gctgttcgaa gtagtactca
     3481 tgcatgcgca tgccatcgaa ctcaaatccc atgcggcggc ggagctcatt gatgacaaac
     3541 gcgttcttct cagcgtcagc agtacggagt tccttgatgg tgtcgaggat gaggtttgtg
     3601 tgctttacgt agccttcgta gagggcgaga tgcacttcaa gctgtttctg agaaaggccc
     3661 ttaagttcag gaatagcgaa tttacgtgcg gtgtaggtca tggtgtcagg attacttatg
     3721 gtctgcagta tatcccgttc tgcggtgata tgctgatgaa tacctaccgt gccttcccga
     3781 cgtcgcattg cgctcctagt cgtactcctc gtgcttgtgg cacttaccgc tgccgtatgg
     3841 tgggccgtgc ttgaagaacg gcctgcctat ctcaccgtat cgtttcttga tgtagggcag
     3901 ggcgacgcca tcctcataga gggacccacc ggcattcaga tgcttatcga tggcgggcga
     3961 gaccgtgcag tactccggga actccccaag cgtatgggac cccttgatcg ttccatcgac
     4021 atagtcgtcg agactcaccc tgacgcagat catatcggcg gactgccgag cgtgtttgag
     4081 cggtatgcag tagggaagtt tatgggtcct gggattggga atgcgacgaa catcacggac
     4141 gcacttatta gtgctattga tacggagccc tcactcgagc aggtactcgc gcgccgcggc
     4201 atgcgtatag atttgggcgg aggtgcgtac gccgatattt tgtatcctga ccgcgatgta
     4261 tcgatgcttg agagtaacga gggctccatt gtgatgcggg tcgtgtatgg cgatacctca
     4321 tttctcctcg ggggcgacgc gccttccata gtagaagacg ccattgcgct tacgtatggc
     4381 actgctatcc aaagtgacgt ccttaaagca gggcatcacg gttctcggta ttcaacggat
     4441 gactactggc tttccattat cgatccaacc gtagtgatta tttctgcagg agccggtaac
     4501 tcgtacgggc atccacatgc ggaggtactg gagcgggtac aaaagagtgg tgcttctctt
     4561 ctctctacca tcgatgaagg gatgattgtc ttagagagcg acggcactga cgttatacaa
     4621 aagtaaaagg ggacagaccg tagtctgccc cttttcctta gtcaaagaag tttcgtttca
     4681 gaggaggtgt gtttcctccg aaacgagcat ggaatacacg gtcgttgtcg atgatgacca
     4741 ctccgggttc gttccactcc tcaataagta atgagttttt cgttctggcg acgattctct
     4801 gcataagctc gtcggcgtga gcagggccac gcatgaggta gccatagatg ccaagacgtg
     4861 cggcaagctc taatgcagcg cttggacgct ttccggcaac aacggcaacc gcctcttcgt
     4921 agccgtcttc atgatcgaag caacgatcgt attgcgcttc ggtaatgcag aaccgagggt
     4981 cgtattcccg gttcttacca atagcactgc gtcgttccgg gtcactaaag tacgcttctt
     5041 caatggcgag gatgcgtgct gcagtgttcg gaagggtgac gacggttact gattcgcgcg
     5101 gtccttctga aaggtttgag agcgcgagga atcgtgcctt catatcttta cccagatggt
     5161 cctggtgggg gagaagcgag tagttgccgc tcgtcttgcc accatagtca gggatgcgtt
     5221 tcgcaatttc gagcgaatta atcctgacga cgtgcgcacc ctcttcgtca aagagtgtgc
     5281 ggacatcgct cggcagcatc agtactttgt agtcttctcg ttccgtttca acgattggga
     5341 aatcgcatga atcgatggta atcatcttcg gtttcggcaa tgccgtatcc tccgagttaa
     5401 tgggagacgc tcgatagaaa gaacgcctac agaaagtgta cctctcttga gggataaacg
//
LOCUS       DAORIN010000037         5451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2882_length_5451_cov_4.755745, whole genome shotgun sequence.
ACCESSION   DAORIN010000037 DAORIN010000000
VERSION     DAORIN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2882_length_5451_cov_4.755745"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2245)
                     /locus_tag="PK109_02070"
     CDS             complement(<1..2245)
                     /locus_tag="PK109_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24353.1"
                     /translation="MKISLSGPAALSGNQKRTRIRIAGLALFVALYIGLPAAVEAAYQ
                     QFLPGTDIYFGEFVYDDDFVATTTAGCTMTVYDPSGSLVVNAATMTVDPNGFYAYSFT
                     PTETRGVWPASMSCGSALNGSLVKLDKTFEISDNVVSSSSVAALVNANTNLAVATASS
                     SLSATLPNSIWAFSGRTLTSFGTLVADIWASGTRTLTGFGTLAADVWTSATRTLTAAG
                     LTSGSLALQSDVTTASSSLGTAITNSQTAINANTNSQILTASTSLASFINTNTNAIVL
                     AASSSLGAQFNVLGATIAGIPAAVWNITTASLSSPGSIGKLIVDNLDVAVSTVSGGGG
                     LTAVDIWTYGTRTLTAATLTSGALALQADVQTASSSIAAIVNANTDAQVAAGTVAVNA
                     HTDIASSSIAAVVNANTDAEVLVASTSLASNINTNTNAIVLAASSSLGAQFSSLASSI
                     ASIPTNVWAAGTRTLTGFGTLAADVWTNGTRTLTGAGLTSGSLATLSDVQTASSSLGA
                     AVANSEAAINANTDAEVLAASTSLASNINTNTNAQVLTASTSLGLSLSSIASSITSIP
                     TNVWAAGTRTLTGFGTLAADVWTNGTRTLTGAGLSSGSLATLSDVQIASSSVAAVVNS
                     NTNATVLAASSSLATSIPVNVWNASVRTLSSFGTLASDVWSAASRTLTAAGLTSGSLA
                     TLSDVQTASSSLGAAITNSETAINANTDAEVLAASTSLASNINTNTNAQVLTASTSLG
                     LSLSSIAS"
     gene            complement(2339..2497)
                     /locus_tag="PK109_02075"
     CDS             complement(2339..2497)
                     /locus_tag="PK109_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRH24354.1"
                     /translation="MFGLGSNELIIIAVILVLLFGATRIPALTKGVADAIRTLRGAFK
                     DEDKNKKV"
     gene            complement(2510..3241)
                     /locus_tag="PK109_02080"
     CDS             complement(2510..3241)
                     /locus_tag="PK109_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRH24355.1"
                     /translation="MDDSAPITERYGEYLEELRRRLYRLALIFVASFFIGVGSTSILL
                     PFFVTLFSVPGVTIIATSPFQLLELTMNTGFFSAIALTLPFALREVYAFLQDALLPRE
                     RRLIFWYIPLALLLFAAGFTYGFAIMYYAIVVIANVNIGFGITNLWNITQFISQILIT
                     AALLGVIFELPLILSGLIQLGVLSVDYLRRHRRIAYAGIFIFVALLPPTDGLSFLVMS
                     LPLVALYEVTIAVNSFRSRKELLTI"
     gene            complement(3265..3447)
                     /locus_tag="PK109_02085"
     CDS             complement(3265..3447)
                     /locus_tag="PK109_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24356.1"
                     /translation="MALTDIDFNEGEGQDFIVELPTGTLGTPGDILVEAGSDAYLLLE
                     TPAGQGGGDEIFIIND"
     gene            complement(3450..4070)
                     /locus_tag="PK109_02090"
     CDS             complement(3450..4070)
                     /locus_tag="PK109_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24357.1"
                     /translation="MITISKGDRVAGLPDLIVSSPSGEYADVDLILCARKDAPNFDAA
                     MYQAQFIRYGEVVYQHETPEALGAVLVAIDPESTHDAAQLWREEEKRRIAREGGTLVP
                     EDQTPAPDAVEPQTTIPEEEEDDVKFFKEKEEEAATTTPDEAVPDREIPVIDIEEPPL
                     GEVGGAASSTEPIADPSVPVIQIDEPVVTPPVEDVPALDMSSTTPQ"
     gene            complement(4073..>5451)
                     /locus_tag="PK109_02095"
     CDS             complement(4073..>5451)
                     /locus_tag="PK109_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24358.1"
                     /translation="VYWLADASTVTNDANSGLALDDRDSWTQQYCYVIERVAATTPSI
                     YKYNFRTALASISAGKTTSAFVFATGIQTVTGNTSATNNGRVGTLSHGPASGIKSLFF
                     ATTSRIYRVAIANIVTGSTTFLSDAMVEVPTGGTGTFTATGALTGVEISDQIDRLIVT
                     STGAAGVRSYVTKYNTNSDPFDHIFLIDSKQIDQAAADSSSVPHPNIQAGIMNVWSEE
                     GLTYICRAGTTSLTNALYAVPLGADWSYADGTVKQRLYTPSLATPNAVKYSRAYVNNA
                     QYLGEDNLTLPTEPMRMYYRTSGISDNSGSWTLIDQTGGLSGVDGAAAIQFAIEFRTI
                     GTFCLPTRVFNVCVVYEDNTTDSHYQPSVAFSSTTAKQFAWRFSTAFGGTVPTLQIQL
                     FDAVSGSLLLTDDSATPTGTWEKSTNDGGAWGAYNTTDKGNDTTYIRYTPAALGDDVK
                     VRALLTQA"
BASE COUNT         1302 a         1309 c         1593 g         1247 t
ORIGIN      
        1 aagatgcgat tgatgaaagc gagagaccaa gggaggtaga agcggtgagt acctgagcat
       61 tcgtgttcgt attgatattt gaagcaagag acgtagaagc ggcaagaacc tcagcatccg
      121 tgttcgcatt aattgccgtt tcgctgttgg tgatggcggc gccaagcgac gagcttgcag
      181 tctgtacatc agacaaggta gcgagtgagc ctgacgttaa ccctgctgcg gtaagggtgc
      241 gtgaggcagc gctccataca tccgatgcaa gcgttccaaa ggaggagagc gtacgcacgg
      301 acgcattcca tacgtttacg ggtatcgaag tggcaagaga cgaagacgca gcaagtacgg
      361 ttgcgttggt gttgctattc acgacagcag ccactgagct tgaggcgatc tggacgtcag
      421 acaacgtcgc aagtgaaccg ctcgagagcc cggcgcccgt gagggttctt gtgccattcg
      481 tccacacatc agctgcgagc gtgccgaatc cggtgagggt acgtgttcca gcggcccata
      541 cattcgttgg gatggatgta atagatgaag cgatagaaga aagcgagaga ccgagcgagg
      601 tggaggcagt gagtacctga gcattggtgt tggtattgat gttggatgca agtgaggtgg
      661 aagctgcaag cacctcagca tcggtgttcg cgttgatagc cgcttcgctg ttagccaccg
      721 ctgctccgag cgagcttgag gccgtttgaa cgtcagacaa cgttgcgagc gagcctgagg
      781 ttaggccagc accggtaagc gttctggtcc cattggtcca cacgtcagca gcaagggtgc
      841 cgaagccagt cagagtacgc gtgcctgctg cccatacgtt ggtaggaatg gatgcgatgg
      901 atgacgctaa cgatgagaac tgagcgccaa gtgatgacga cgcggcaagg acaatcgcat
      961 tcgtgttcgt attgatattg gacgcaagcg aggtggaagc cacgaggact tcagcgtcgg
     1021 tgttggcatt cacgaccgct gcaatggaac ttgaggcgat gtccgtatga gcattcacgg
     1081 ctactgttcc ggcagcaact tgcgcgtcgg tattagcatt tacgatagcg gcaattgaag
     1141 aggaggcggt ctggacgtca gcttggagtg cgagggcacc tgaggtgagg gttgctgcag
     1201 taagcgttcg ggtgccatac gtccagatgt ccactgcagt gaggccacca ccacccgaaa
     1261 ccgtcgatac cgcgacatcg aggttatcca cgatgagctt ccctatcgat cctgggcttg
     1321 agagcgatgc ggtagtgata ttccagaccg ccgccggtat gcctgcgata gtcgctccca
     1381 ggacgttgaa ttgcgctccg agcgagcttg aagcggcgag cacaatcgcg ttggtgttcg
     1441 tattgatgaa cgacgcgagc gatgttgagg cggtaagtat ctgtgagttc gtgttcgcat
     1501 taatagccgt ttgactgttc gtgatggccg tgcccagtga cgaacttgcg gtggtgacat
     1561 ctgactgaag agccagtgat ccggaggtga gaccggctgc ggtgagtgta cgtgttgcgg
     1621 atgtccacac gtcagccgca agggtgccaa atccggtaag ggtgcgagtg ccggatgccc
     1681 agatgtcagc tactaatgtt ccgaacgatg taagagtgcg acctgagaat gcccaaatgg
     1741 aattaggaag ggttgctgag agcgaagagg aagcggtcgc aaccgcaaga ttcgtattcg
     1801 catttactag ggcagcaacg gacgaagagg aaacaacatt gtctgatatc tcaaacgtct
     1861 tatcgagctt caccagactt ccgtttaatg ccgatccgca ggacatggat gcgggccata
     1921 ccccccgggt ttcggttggt gtaaaggaat aggcgtagaa gccattgggg tcaacggtca
     1981 ttgttgccgc attcaccaca agtgagccgg acggatcata gaccgtcatg gtgcagcctg
     2041 cagtagtagt tgctacaaag tcatcgtcgt atacgaattc accaaagtag atatcagttc
     2101 ctggaaggaa ctgctggtac gcagcttcta cggcggcggg aagtcctata tatagcgcaa
     2161 caaacaacgc taggcccgca atgcgtatcc tagtcctttt ttgattcccg cttaaagcgg
     2221 cagggccgct taatgaaatc ttcactttta cagtgtagca ctgcctctag agcactcttc
     2281 ccttttcccc acaagaatac aagaaaaatc ccggctagga ccgggatttt tctttgtgct
     2341 ataccttctt gtttttgtct tcgtctttga atgcgccgcg cagtgtgcga atagcgtctg
     2401 ctactccttt cgtgagcgct gggatgcggg tggcaccaaa caaaagtacg agaatgaccg
     2461 cgatgatgat gagttcattc gagccgagtc caaacatagg taccaaaaat tatattgtta
     2521 agagttcttt ccggctgcgg aacgaattga cggcaatcgt cacttcgtag agcgccacaa
     2581 gcggaaggga catcaccagg aaggatagcc cgtcggttgg gggaagtaaa gcaacaaaga
     2641 tgaatatccc tgcatatgcg atgcgacgat ggcggcgcag gtagtctacc gacaacacgc
     2701 cgagctggat aaggccagaa aggattaacg gaagctcgaa gatgacaccg aggagtgctg
     2761 ctgtgataag gatttgtgag atgaattggg ttatattcca gagattagtg ataccgaacc
     2821 cgatgttcac gttcgcgatg accacgatag cgtaatacat aatcgcaaat ccgtaggtaa
     2881 acccggcggc gaagagaaga agggcgagcg gaatgtacca gaaaataaga cgccgttcac
     2941 gtggtaagag cgcgtcttgg agaaaggcat agacttcacg aagcgcaaac ggaagggtga
     3001 gcgctatcgc cgagaaaaaa ccggtattca tcgtcagttc aagaagttga aacggcgagg
     3061 tggcaatgat ggtcactccc ggcacagaga agagtgttac gaaaaaaggc aacaagatac
     3121 tggtagaacc aactcctatg aaaaaagaag caacgaaaat aagcgcaaga cggtagagtc
     3181 tgcgccgtag ctcttcaaga tattcgccat agcgttccgt aatcggcgct gaatcgtcca
     3241 tgccctgaag gataccagac ctggttagtc gtttatgatg aatatttcat ccccacctcc
     3301 ctgccctgct ggggtttcta ggagaagata cgcatcgctc cctgcttcga ccagaatatc
     3361 tcccggcgta cccagggttc cagtcggaag ctcgacgatg aaatcttgtc cttctccttc
     3421 gttaaagtcg atatcggtta gggccatggc tattgcggtg ttgtgctcga catatcaagc
     3481 gctggtacat cttcaaccgg aggagtcacc actggctcgt caatttgtat gacggggact
     3541 gaaggatcgg cgattggctc tgtagaagat gctgcaccac ctacctcccc gagtggcggt
     3601 tcttcaatat caatgaccgg gatttctctg tccggcaccg cttcatctgg tgtggtagta
     3661 gctgcttctt cctctttttc cttaaagaac ttcacatcgt cctcctcttc ctcaggaata
     3721 gtcgtctgag gttcaacggc atcgggtgca ggagtttgat cttctggcac tagagtaccg
     3781 ccttcacgtg cgatgcgacg cttctcttct tcccgccaca gctgcgcagc atcgtgagtg
     3841 gattctggat caatggcgac aagcaccgct ccaagcgctt ccggtgtctc gtgctgatag
     3901 acgacttctc cgtaccggat aaactgcgct tggtacatgg cggcgtcgaa gttcggcgcg
     3961 tccttccgcg cacagagaat aaggtctacg tctgcgtact ctcccgacgg agaggacact
     4021 atgaggtcgg gaagtcccgc gacacgatca ccctttgaga tagtaatcat aattaggctt
     4081 gggtaaggag ggcacgcact ttcacatcgt cgccaagtgc tgcaggggtg tagcggatat
     4141 aggtcgtatc atttcccttg tcagtggtat tgtacgcgcc ccacgctccc ccatcgtttg
     4201 tagatttttc ccaggttccc gttggagttg cgctgtcatc ggtgagtagg aggcttcctg
     4261 aaacagcgtc gaacagctgt atctgtaagg taggcacggt gccgccaaag gcagtcgaga
     4321 agcgccatgc gaactgctta gcagtggttg aagagaacgc taccgatggc tggtaatggc
     4381 tatcggtcgt gttgtcttca tatacaacac atacgttaaa cacgcgagtt ggtaaacaga
     4441 acgtaccaat cgtgcggaac tcaattgcga actgtatggc ggccgcgcca tcaacaccgg
     4501 aaaggccccc tgtttggtcg ataagcgtcc atgagccgct gttatcgctt atacccgaag
     4561 ttcggtaata catgcgcatc ggttcggtcg gaagcgtgag attatcttct cccaagtatt
     4621 gcgcattatt tacatacgca cgcgaatact taaccgcatt aggcgtcgct agggatgggg
     4681 tgtaaagccg ctgttttacc gtgccgtcgg cataggacca atcagcgcca agcggcacgg
     4741 cgtagagtgc gtttgtaagc gaagtggtac cggcgcggca gatgtacgtg agtccctctt
     4801 ctgaccagac gttcatgata ccggcctgga tgttggggtg tggcactgag cttgagtcgg
     4861 ctgccgcctg atctatttgc ttagagtcaa tcaggaagat atggtcgaaa ggatcggagt
     4921 tagtgttgta cttggttacg tagcttctca cgcccgcagc tcccgttgag gttacgataa
     4981 ggcggtcaat ctggtctgaa atttcaacac cggttaatgc gccggtggcc gtaaaggttc
     5041 cggtgcctcc ggtcggtact tccaccatgg cgtcagaaag aaatgtggtc gaaccggtta
     5101 ctatatttgc gattgcaacg cggtaaatgc gcgaagtcgt tgcgaagaag agagacttta
     5161 ttccggaagc cgggccgtgg gaaagtgtgc cgacacggcc gttgttagtt gctgatgtgt
     5221 taccggtaac ggtctggatg ccggttgcaa acacgaacgc agaggtagtt ttcccggcag
     5281 atattgacgc aagcgcagtg cggaaattgt atttgtatat tgacggcgtg gtcgcggcga
     5341 cgcgctcaat gacatagcag tactgctggg tccaactatc ccgatcgtca agcgcaaggc
     5401 ctgagttcgc gtcgttggtg acggttgagg catcggcaag ccagtacact g
//
LOCUS       DAORIN010000038         5373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_2962_length_5373_cov_4.348815, whole genome shotgun sequence.
ACCESSION   DAORIN010000038 DAORIN010000000
VERSION     DAORIN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2962_length_5373_cov_4.348815"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PK109_02100"
     CDS             complement(<1..871)
                     /locus_tag="PK109_02100"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HRH24359.1"
                     /translation="MNDFKKHFQLIREDLSLTDGEHSLMRAELERRAMPAVGMVASPF
                     YFFAGPFRVAGAFAVLLFVLTGGSSVLANEALPGDALYSVKIHLNENVERSLARSPLA
                     KADVDIKHAEERLTEVELLAASGKAEEEVVEDAAAKVEAKVAAAALTADTLSDEGDEA
                     AADSIHTRINATLLAHADILDAQAADLEEETGQSLRALSVAVTFAVDEAAEVRDEGVE
                     DTGDDGALATAVALDREAGAKERIEALTEALAEEDVPEETQELLATELASLEADYGAN
                     RERMAEEEYEEAAD"
     gene            complement(868..1383)
                     /locus_tag="PK109_02105"
     CDS             complement(868..1383)
                     /locus_tag="PK109_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRH24360.1"
                     /translation="MRSSSTIYRDDYLAAYDEYADAVFRMCYGKTSNREEAKDMTQEV
                     FTRVWQRVSSGKEEIENLRAFIFTVARNLIKDYYKRKKPVLERDLPEGAMESIPTAPD
                     QSMRSESALMLEALKELKDPYREALMLHLVEGIPIGEIAELLGERPNTISVRVKRGVE
                     KVRVMLKIETP"
     gene            complement(1479..2546)
                     /locus_tag="PK109_02110"
     CDS             complement(1479..2546)
                     /locus_tag="PK109_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRH24361.1"
                     /translation="MGKILTGEFLTFDDVLIYPAASAIEPKTAQVATQLAKGFNIQIP
                     IISAAMDRVSDAKMAVALSALGGLAIIHRNNTAVEEAAMVKEAKKKGARVGAACGPFD
                     IERAKLLVKAGADLISIDSAHGHNANVIAGAKKIKKAIGKVPLLVGNIATKEAALELV
                     KFADGIKVGIGPGSICTTRIVSGVGVPQLTAVMDVAAVGKKYGVPVIADGGIRTSGDI
                     SKALAAGASAVMLGGLLAATDAAPGKVVTMNGKKFKEYRGMGSMDVLKEKNANDRYLG
                     DAKGMVPEGVSGLVPYTGTLEEVVNRLVGGLKVSMGYVGAKNLAQHRARTRFIKVSKA
                     AVIEGRPHSLAGTKEQEDLLP"
     gene            complement(2772..3884)
                     /locus_tag="PK109_02115"
     CDS             complement(2772..3884)
                     /locus_tag="PK109_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24362.1"
                     /translation="MTEGFKEQANGSVETSEQYDFPLLEKVAEHYRSLPHEVGETYLV
                     CCQHLLEPQKHMFEKLISFGFDPLKIRILGKIYSTNEEIQTELSEMGINAIQPSFTGA
                     SFDNEHQENCREMINEIPNDSPVILLDDGGQLITESVVSGKRILFAVEQTSSGYRKLE
                     GKEIPFPVLNVARSTTKLVQESPFIARHACERMKTYFIENGLPKPTILIVGLGPIGEA
                     VQQYFVREGFSVSGVDSKHGHTDLVATINAQKPDIVIGATGSNILTPEEMEKISVDSA
                     VHFISVSSSDREFPVVAYREGQMPAHGDVRYKNFTFVNNGFPITFKGYKNEAYPKEME
                     KTMSLLSGAVMHGATASTLQNGLIDLPEELEKLVNE"
     gene            complement(3895..>5373)
                     /gene="guaA"
                     /locus_tag="PK109_02120"
     CDS             complement(3895..>5373)
                     /gene="guaA"
                     /locus_tag="PK109_02120"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRH24363.1"
                     /translation="VRDMGVRAEILPHDATAADIRAFNPSGIILSGGPSSVLEKGSPK
                     PDKEVFTLGVPVLGICYGHQTMAHMLGGKVEKGKHREFGKETLSVSSKTGLFKGLSAK
                     ETVWFSHGDTATKLPKGFVVTASTSSCKYAAFADVKRNLYGIQFHPEVTHTKNGKTVL
                     KNFVLDICAAKKDWNMKTVARDIIERVKEEVGPEEVVVGISGGVDSLVAATLIHRAIG
                     NRLHAVFVDTGLLRKGEVEQVAGFLRKNGFKKLHVIDASKLFLTRLKGVSDPEQKRKI
                     IGHSFIEVFEKAIKKELGGKKVAYLAQGTIYPDRIESAATGKGAAKIKSHHNLTLPEK
                     LGFGILEPIKDLYKDEVRRVGATLGLPHELLWRHPFPGPGLAIRIVGEITPERLSILR
                     EADAVYIEELKKAGEYEKIWQAFAALLPVKVVGVMGDARTYEYVIALRAVDSVDGMSA
                     DWHKMPHGLLEKISGAIVGKVRGANRVLYDVTQKPPATIEYE"
BASE COUNT         1190 a         1483 c         1324 g         1376 t
ORIGIN      
        1 cgtctgccgc ttcctcgtac tcttcttcgg ccatgcgctc acggttggcg ccatagtcgg
       61 cttcgagcga agcaagttca gtcgccagga gttcctgagt ttcttccggc acatcctcct
      121 cagcaagcgc ctcggtcaag gcctcgatgc gctcttttgc tcctgcttcg cgatcaagcg
      181 cgaccgcggt tgcgagcgct ccatcatctc ccgtatcttc cacaccttcg tcacgcacct
      241 cggcagcttc atcaacggca aaggtgacgg caactgaaag cgcgcggagc gactgtccag
      301 tctcttcctc aaggtcggca gcctgtgcat ccagaatatc tgcgtgggca agaagcgttg
      361 cgttgatgcg tgtatgaata ctgtcggcag cagcctcatc gccctcgtca gagagcgtgt
      421 ctgcggtaag ggcggcggca gcgactttcg cttctacttt tgccgctgca tcttcaacca
      481 cttcttcttc tgctttcccc gaagcggcaa gaagctcaac ttcggtaagg cgctcttctg
      541 cgtgcttaat atcgacatct gctttggcga gcggcgaacg tgcaagcgag cgttcaacgt
      601 tctcgttcag atgaatcttc acgctgtaga gtgcatcgcc cggaagtgct tcgtttgcta
      661 agaccgagct tccaccagta agtacaaaaa gaagtacggc aaaggcgcca gcaacgcgga
      721 aggggccggc aaagaaatag aacggagacg ccaccatgcc cactgccggc atcgcacggc
      781 gctcaagctc cgcacgcata agggaatgct ccccgtcggt gagcgaaagg tcttctcgaa
      841 tgagttgaaa atgttttttg aaatcgttca tggtgtttct atcttgagca tcacacgcac
      901 tttctctaca cctcgcttca cccgcactga aatggtgttg ggacgctcgc ctaaaagttc
      961 tgctatctcc cctattggta tgccttcaac cagatggagc atgagcgctt ctcgatacgg
     1021 atccttgagt tccttcaggg cctcgagcat gagggcgctt tcgcttcgca tgctttggtc
     1081 aggagccgtt ggaatggatt ccatggcacc ttccggtaag tcacgctcaa ggactggttt
     1141 cttacgcttg tagtaatcct tgatgagatt acgagccacc gtaaagatga aggcacgcag
     1201 gttctctatc tcctctttcc cgcttgagac ccgctgccat acgcgggtga atacctcctg
     1261 cgtcatgtct ttggcttctt cgcggttaga cgtttttcca tagcacatgc ggaataccgc
     1321 atccgcgtat tcgtcatacg ccgctaggta gtcgtcccga tagatagtgc tgctgctccg
     1381 cataagataa cgattgactg taccttatat tacagggttt ttgatagatg tggggtgaag
     1441 aacacgaaga aacgccctat ctaggcggtg agacgttgtt aggggaggag atcttcctgc
     1501 tccttcgtac cggcaagtga gtgcggacgt ccttcgataa ccgctgcttt tgacaccttt
     1561 atgaagcgcg tgcgagcgcg atgctgcgca aggttcttag cacccacgta tcccatggat
     1621 accttaaggc cgcccaccaa ccggttcact acttcttcta gggttccggt gtacgggacg
     1681 agtcctgaca cgccttcagg caccatgcct ttggcgtccc cgaggtagcg gtcgttcgca
     1741 ttcttttctt taagcacatc catggaaccc atgccacggt attccttaaa cttcttaccg
     1801 ttcatcgtca ctactttccc cggtgcagca tccgtcgcag ccagaaggcc gccgagcatg
     1861 acggctgagg cgcctgcggc aagtgcttta gaaatatcgc ctgaggtgcg tatgccgccg
     1921 tcagcgatga ccgggactcc gtacttcttt ccgacagcgg cgacgtccat aacggcagtg
     1981 agctgaggaa cgccgacccc tgaaacgatt ctcgtggtgc agatagagcc cggacctatc
     2041 cctaccttga taccgtcagc gaacttcacc aattcaaggg cagcttcttt ggtggcgatg
     2101 ttccctacca gaagcggtac cttccctatc gctttcttaa tcttcttagc gcccgctatc
     2161 acgttggcgt tatgaccgtg cgcggagtcg atggagatga gatcagcacc cgcttttacg
     2221 agaagtttcg cgcgctcaat atcaaacggg ccgcaggccg cccccacgcg tgcacctttc
     2281 ttcttagctt ccttcaccat tgctgcttct tcaactgcgg tgttgttgcg gtggatgatg
     2341 gcaaggccgc cgagcgctga aagcgcgacc gccatcttgg cgtctgagac acgatccatg
     2401 gctgccgaga taatcggaat ttgaatattg aaccctttcg caagttgagt cgcaacctgc
     2461 gcagtcttag gttctatggc gcttgccgcc ggatagatga gtacgtcgtc aaaggtgagg
     2521 aactcaccag taagtatctt tcccatatcc gactatacat cggagaggtt tcaggcacaa
     2581 atgcctgggg ataaagctag gtctaaatcg cagcgaagtt cccgagcgag tccgtagtcc
     2641 taaagaccga cggtgcctga cgcgagtcct cccagcaaga taaacaaaag cagatgtctc
     2701 cgtggttgct gtgcatacca ccgtagagta cagagaaggc agtgaaatca atatgaattt
     2761 gcagctccta ttcactcatt tactaacttc tcaagctctt caggtaaatc gataagaccg
     2821 ttttgtagtg tggaagcggt agcgccgtgc ataacggcac cactgagtag tgacatcgtt
     2881 ttttccattt ccttaggata tgcctcgttc ttgtatcctt tgaaagtaat cgggaaaccg
     2941 ttattcacga acgtaaaatt cttataacga acatcgccgt gagcgggcat ctggccctcc
     3001 cggtatgcca caaccggaaa ttctctgtcc gacgaagata cgctgatgaa atgaactgcg
     3061 ctgtctactg atattttctc catctcttca ggggtgagga tatttgatcc tgtagcacca
     3121 ataacgatat ccggcttctg agcatttatg gtcgcaacga gatcagtatg tccgtgctta
     3181 ctatcaacgc ctgatacgga aaaaccctct cgcacaaagt attgctgtac cgcttcaccg
     3241 atagggccga gaccgacaat tagaatagtg ggcttgggta ggccattttc aatgaagtag
     3301 gttttcatac gctcgcatgc atgtcgtgcg atgaatgggc tttcttgcac gagttttgta
     3361 gtggaccgtg caacgtttaa taccgggaac ggtatctcct tcccttcaag ttttctgtac
     3421 cccgacgacg tctgttcgac agcaaataag attcttttcc cgctcaccac gctttcagtt
     3481 ataagttggc caccatcatc caaaagaatt acaggagaat catttggaat ttcgttgatc
     3541 atctccctgc aattctcttg atgctcgtta tcgaacgagg ctccggtgaa agagggttgt
     3601 atagcgttaa ttcccatctc gctaagctca gtctgaatct cttcgttcgt agagtaaatc
     3661 tttcctaaga tccggatttt taatggatcg aaaccgaaag aaatgagttt ctcgaacata
     3721 tgtttctgag gctctagtag atgctgacag cagacaaggt aggtctctcc aacctcatgc
     3781 ggcaacgaac gatagtgctc cgcaaccttt tctagtagag gaaaatcata ctgttcactg
     3841 gtttcaacac tgccgtttgc ctgctcctta aaaccctctg tcataaagta atgattactc
     3901 gtactcaata gtagccggcg gcttctgagt cacatcgtag aggacacgat tcgcccccct
     3961 gaccttcccc actatcgcgc ctgatatctt ttccaagaga ccgtgtggca tcttatgcca
     4021 gtcagcactc atgccatcaa cggaatccac ggcacgtagc gcgatgacgt actcataggt
     4081 gcgcgcatct cccatcacgc ccaccacctt cacgggaaga agcgctgcaa atgcttgcca
     4141 gatcttttcg tactctcctg ccttcttgag ttcctctata tagacggcgt ccgcttcacg
     4201 aagaatggaa agacgctcag gcgtaatctc accgacgata cggatggcaa ggcctggccc
     4261 ggggaacgga tggcgccaca aaagctcgtg cgggagtcca agcgttgcgc ccacccgacg
     4321 cacttcgtct ttgtacaagt ccttaatcgg ctcgagaatg ccaaagccaa gcttctcagg
     4381 aagcgtgagg ttatgatggc tcttaatctt cgctgcacct ttgccggtag cggcgctttc
     4441 gatgcggtct ggataaatgg tgccttgtgc caggtacgcg actttctttc ctccgagttc
     4501 tttctttatc gccttctcga atacttcgat aaaggagtgg ccgatgatct tacgcttctg
     4561 ttcgggatca gacacgccct taagtcgcgt caggaagagt tttgacgcat cgatgacgtg
     4621 gagcttcttg aaaccgtttt tgcgtaagaa acctgcaacc tgttccactt cccctttacg
     4681 caggagcccg gtatcaacaa acaccgcatg gagacggttg ccgatagcgc ggtgaattaa
     4741 agtagccgca acgagcgagt ccacgccgcc tgagataccc accactactt cttctggccc
     4801 cacttcttct ttcacacgct cgatgatgtc acgtgcgacc gtcttcatgt tccagtcttt
     4861 ctttgccgca caaatgtcga gtacgaaatt cttcagcacg gtttttccat tcttggtgtg
     4921 cgtcacttct gggtggaact gaataccgta gaggttacgc ttcacgtcag cgaatgccgc
     4981 gtacttacag gaactcgttg atgcagtcac gacaaaccct ttcgggagct tcgttgccgt
     5041 gtctccatgc gagaaccaga ccgtttcttt tgcggagagt cctttaaaga gtccggtctt
     5101 tgaagaaaca gaaagggttt cttttccgaa ttcacgatgc ttccccttct ctacttttcc
     5161 gccgagcata tgtgccatcg tctggtggcc atagcatatg ccaagtaccg gaacgccgag
     5221 cgtgaatact tccttgtcgg gctttgggct tcctttctca agcactgaag agggaccacc
     5281 cgaaaggatg atgccggatg ggttaaaggc acggatgtcg gcggcagtcg cgtcatgcgg
     5341 gagaatctcc gcacgtaccc ccatatcacg gac
//
LOCUS       DAORIN010000039         5274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_3053_length_5274_cov_4.603564, whole genome shotgun sequence.
ACCESSION   DAORIN010000039 DAORIN010000000
VERSION     DAORIN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_5274_cov_4.603564"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PK109_02125"
     CDS             complement(<1..187)
                     /locus_tag="PK109_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24364.1"
                     /translation="MTALQKSDNEYDVLVVERIPNGDTLAFTYGKHIAFDKIPVHVLF
                     PQSSVPDAILEVGFRHEG"
     gene            258..527
                     /locus_tag="PK109_02130"
     CDS             258..527
                     /locus_tag="PK109_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRH24365.1"
                     /translation="MTSSKKKWTVYILECQDGSLYSGITTDMERRLAEHNGIGARGAR
                     YTRARRPLQLVHTERFATRSAASIREAALKKLTRSEKLALIKRNA"
     gene            543..1808
                     /locus_tag="PK109_02135"
     CDS             543..1808
                     /locus_tag="PK109_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24366.1"
                     /translation="MINGPEKAPRRKFTPVDEVEEKKMSRRTLLAGLGLAGVTAAGVT
                     RVMTSRISTEPLAEAAETIENIIESIPGVSSERESIPEGPYDTGPLAGRTLGNLFAMY
                     LGLPDPSVVPERLSANFPQILSDLWRKKREYVMRHKPEWIDNTRRAEHRFLEEYRAWR
                     EGERDRGPKDLNEFNRSLQPVVHDIHAAVNFSRLNELAPFRRLSEEDTDLVKRLAGNI
                     SSKTLIAYGITELMPSVNDSALNVDMTEFLIKNAGAPFLAAPPAIHDPYVSAGLYQFT
                     SHAVYAGEEGRRGASLINQLVTERDQRIPDSVIGLQTIEQQTRAAYLFAVENLARLIV
                     TIRAGLDRNKRDERLRTLRDHADTLDIGTLQFIASAHHNPSQARRGFIKWLDKGLDGE
                     HSIHCDSRIKDYIIKTNGIYNELSRRVRS"
     gene            1827..2237
                     /locus_tag="PK109_02140"
     CDS             1827..2237
                     /locus_tag="PK109_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRH24367.1"
                     /translation="MSPLEMQFLVGLGVSIVIGLVIGVERESRNKDAGISTHILVIAG
                     AMLFTQLSAIVDPASTSRIASQIIPGIGFLGAGLILKEGGNVRNLTTAASLWFSAAIG
                     MAIGFGFITIAAIAGIACALIPRIPSVHKHPIEG"
     gene            2279..2698
                     /locus_tag="PK109_02145"
     CDS             2279..2698
                     /locus_tag="PK109_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005383230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRH24368.1"
                     /translation="MKNLLQCTCASKWGEAAPLILRVVTGVIFFMHGYQKLMVMGIPG
                     VTGFLTMLGMPFPGVLAVLLIAGELLGGLFLILGLWTHWSAKVLAFIALVALFAVHFS
                     KGFFLPGYEFILLLLAASVSLMITGPGKWSVDRKMMK"
     gene            2789..3415
                     /locus_tag="PK109_02150"
     CDS             2789..3415
                     /locus_tag="PK109_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24369.1"
                     /translation="MTITKSLIAFATLIILMPSGAAAASLYGGDTGAVSVTIGADVSA
                     GASATGSPSSSSAGDDSSMPNTPSTTGLNVSVTSASSVTTEMDLADYRESVMEEDAAV
                     AEVDAVAGSDVTVSYWHEGRLFGIFPVKVKSETTAALTTDGEVAVTTHMPWWNMFVTG
                     TGDARAAVDEELSSSTRVAADLKMKEDVAARARLIEAMVAAHAAVHAK"
     gene            3458..4054
                     /locus_tag="PK109_02155"
     CDS             3458..4054
                     /locus_tag="PK109_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRH24370.1"
                     /translation="MNAFPDTFSIIDTETTGMHPSYARVIDIGIIRVEKGEVVERYET
                     LVNPGEPVPRPITRLTGITDQMLVGAPVFADVALEVEKLLSGAVFVAHNASFDYGFIK
                     HEFARAGIEWSAESLCTVTLSRQLFPGERSHSLEAIIERYGIGTDERHRALPDAEAVL
                     EFMQSVSREVEPRVLERALRIKESGTRPKYPTEERFIT"
     gene            4073..4486
                     /locus_tag="PK109_02160"
     CDS             4073..4486
                     /locus_tag="PK109_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRH24371.1"
                     /translation="MTFIQSLSVYALSVPVFFAIDMLWIGLIASGFYKSNLGHLMSST
                     VNWPVAIVFYLLFLIGLLIFAIAPALAQKSLMHAILFGALFGFFTYATYDLTNWATLK
                     DWPPIVALVDMVWGTVLSASVAGLSYLLAVTFVVK"
     gene            4489..5262
                     /locus_tag="PK109_02165"
     CDS             4489..5262
                     /locus_tag="PK109_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRH24372.1"
                     /translation="MTILLTALAVLLIYMSGLFVIATLAKNNGVADIGYGIAFIVLIG
                     TTLIVSSSVSFYVLLLSFLPLVWGVRLATRIYRKNKGKPEDFRYKAWRDAWGTSFLFR
                     SFLQVYMLQGLVVFVVALPVTLAIAYPTLVVMPALVWVGVALWCVGFFFEAVGDAQLD
                     AFLKNPVNKGKIMTSGLWRYSRHPNYFGESLMWWGIALACSALSSVPLVGFIGPILIT
                     FLLLKVSGVPLLEKHFDGNPEWEAYKARTPVFIPLPPKV"
BASE COUNT         1136 a         1398 c         1424 g         1316 t
ORIGIN      
        1 gaccctcatg gcgaaacccg acttcgagta ttgcgtcagg aactgagctt tgagggaaca
       61 agacgtgtac gggtattttg tcgaaagcga tgtgcttgcc gtaggtgaat gcgagcgtgt
      121 cgccgttcgg tatccgctct accaccagca catcgtactc attatcactt ttctgcaatg
      181 cggtcatggg atctcctccg taatgacacc ccttcgttat agcgcatagc gcagggacat
      241 gccaccatac tccctgcgtg acttcatcta agaagaaatg gacggtctac attctcgagt
      301 gtcaggatgg ctctctgtat tcaggcataa ccaccgacat ggagcgacgc ctggccgaac
      361 ataacggtat aggcgcccgt ggcgcccgat atactcgagc gcggcgccca ttacagctcg
      421 tccatacgga gcgctttgcc acacgaagcg ctgcctctat acgtgaagca gcgcttaaaa
      481 agctcacgcg ttctgagaag ctcgcgctta tcaagcgcaa cgcctgatgc taccctagga
      541 acatgattaa tggtcccgag aaagcaccgc gccgtaagtt tacgccggta gacgaggtgg
      601 aagagaagaa gatgagcagg cgtacgctcc ttgcgggtct tggacttgct ggcgtcactg
      661 cggccggggt gacgcgtgtg atgacaagcc gcataagtac ggagccgctt gcagaggcgg
      721 ccgagacgat agagaacatc attgagtcta ttccaggagt ctcttcggaa agagagtcca
      781 ttcctgaagg gccttacgat accggcccgc tcgcggggcg cacgctcggg aacctgtttg
      841 ctatgtatct tggtcttcct gatccgagcg tggtgcccga gcgcctttcg gcgaacttcc
      901 ctcagattct ttctgacctt tggaggaaga agcgggaata cgtgatgcgg cataaaccgg
      961 agtggataga caatacgcgg cgtgcagaac accgattcct agaggagtat cgcgcatggc
     1021 gggaagggga acgagaccga ggtccaaaag acctcaatga gttcaatcgt tctttacaac
     1081 cggtggtaca tgacatacat gcagcggtaa atttctcgcg attgaatgaa cttgcaccct
     1141 tccggaggct ttcggaagaa gataccgacc ttgtgaagcg acttgcggga aatatatcgt
     1201 cgaagacgct catcgcatac ggcatcactg aacttatgcc gtcggtgaac gattctgctt
     1261 taaacgttga tatgacagag tttcttataa aaaacgcggg agcaccgttt cttgctgctc
     1321 ctcctgcaat ccacgatccc tatgtttccg ctggcctcta tcagttcacg tcgcatgccg
     1381 tgtacgcggg agaagaaggg cgccgtggtg cgtctctcat aaatcagctg gtgactgaga
     1441 gagatcaacg catccctgat tcagtcatag gtctccagac catagaacag cagacgcgcg
     1501 ccgcatactt gtttgcggta gaaaatcttg cacgacttat cgttactatc cgtgcgggac
     1561 tcgataggaa taagcgcgat gaacgcttac gcacgcttcg tgatcatgca gacacattag
     1621 atataggaac gctccagttc atcgccagcg cccaccataa tccaagtcag gcgcgtcgcg
     1681 ggttcatcaa atggctcgat aaaggccttg atggagagca ctccatacac tgcgatagcc
     1741 gaatcaagga ctacattatt aaaaccaatg gcatatataa cgaactcagt agacgagtgc
     1801 gctcatagtc ctgctatact tttcatatgt cacctctaga aatgcagttc ttggtagggc
     1861 tcggggtttc cattgttatt ggtctcgtta tcggtgtcga gcgagagagc cgcaacaaag
     1921 atgcaggtat ctcaactcac atcttggtta tcgcgggtgc catgctcttt actcaacttt
     1981 cggcgatagt ggatccggcc tctacctcac gcatcgcatc tcaaatcatt ccgggtatcg
     2041 gtttccttgg cgctggcctc attcttaaag aaggaggcaa tgtgaggaat ctcactaccg
     2101 ctgcctcact ttggttctca gccgctattg gcatggccat agggttcggg ttcatcacta
     2161 ttgccgctat cgccggcatc gcctgcgccc ttatccctcg tatcccgagc gtccataagc
     2221 acccgattga aggataagcg ggaggtatat acttataggg tactcatcta atacacctat
     2281 gaagaacctt ctacagtgca cctgtgcaag taagtggggc gaagcagctc cccttatcct
     2341 tcgtgttgtt accggcgtta tcttctttat gcacggctat cagaagctga tggtcatggg
     2401 cattccgggc gttaccggat tcctgaccat gcttggtatg ccgttcccag gagtcctcgc
     2461 cgttctcctc atcgccggag aacttcttgg cggtctcttc cttatccttg gtctttggac
     2521 gcactggtca gcaaaggtgc ttgcgttcat tgctctggtc gcactctttg cggtgcactt
     2581 ctcgaaaggc ttcttccttc cgggatacga gttcatcctt cttctcttgg cggcttccgt
     2641 atcgctcatg attacgggtc ccgggaaatg gtcggtggat cgtaagatga tgaaatagta
     2701 gagcagtatg tgtgcgagaa gaacggcccc attggggccg ttcttctttc atccagcatt
     2761 catgaagtcc ttggtacgtt aggtacgtat gactatcacc aaatcactta tcgcatttgc
     2821 gaccttgatt atccttatgc cgtctggcgc cgctgcagca agtctgtacg gcggagatac
     2881 gggcgcagta agcgtcacta taggcgctga cgtgagcgcg ggcgcatctg ctactggctc
     2941 gccgagctcg tcttctgccg gcgacgattc ttcgatgccg aacacgccat ccaccacagg
     3001 tctcaatgta tcggtcacga gcgcaagcag cgtgactacc gagatggatc ttgctgacta
     3061 tcgcgaaagc gttatggagg aagacgctgc agttgcagaa gtagacgccg ttgccgggag
     3121 cgacgtaacg gtttcctatt ggcacgaggg gcgtctgttc gggatattcc ccgtgaaggt
     3181 taagtcagaa acgacagcag ccttgactac ggacggggaa gttgccgtca ccacgcatat
     3241 gccgtggtgg aatatgtttg ttacgggtac cggggacgca cgcgccgccg ttgacgaaga
     3301 actctcttcc tcaacgcgcg ttgccgctga tctcaaaatg aaagaggatg ttgccgcacg
     3361 tgcgcgtctc attgaagcga tggtcgcagc gcacgctgcg gttcacgcga aataacgttc
     3421 gtttgccggg cactggtgtg acgcgtagaa tcgatagatg aacgcgtttc ctgacacctt
     3481 ctccatcatc gacacggaaa ccacgggcat gcatccctcg tatgcgcgcg tcattgatat
     3541 tggcatcatt cgagtagaaa agggggaagt ggtagagcgc tacgagacct tagtgaatcc
     3601 gggcgaaccg gttccacgtc ccatcacgcg tttgacggga attacggacc aaatgttggt
     3661 gggtgcccca gtgtttgcgg atgtggcgct agaggtggag aagctccttt caggcgcagt
     3721 gtttgtcgcg cacaacgctt cctttgacta tgggttcatt aagcatgagt ttgcacgcgc
     3781 tggcattgaa tggagcgcag agtcgctctg cacggtgacc ctttcacgtc agctgtttcc
     3841 cggggagcgc agccatagcc ttgaagcgat tatcgagcgc tatggcatag ggaccgatga
     3901 gcggcaccgc gcccttcctg acgctgaggc ggtcttagag ttcatgcaga gcgtgtcgcg
     3961 agaagtagag ccacgcgtcc ttgaacgcgc gcttcgtata aaagaaagtg gaacgcgtcc
     4021 gaagtatccg accgaagagc gcttcatcac ctaggcctgc tatcatagag atatgacctt
     4081 catccaatcg ttgtccgtgt atgcgctttc ggtgccggta ttcttcgcca tcgatatgct
     4141 gtggatcggc cttatcgctt cagggttcta taaatcaaac cttgggcacc tgatgtcgag
     4201 cacggtgaac tggcctgttg cgattgtctt ttacctcctg ttcctcatcg ggctccttat
     4261 cttcgccatt gctccggcgc tcgctcagaa gagtcttatg cacgcgattt tgttcggagc
     4321 tctcttcggg ttctttacgt acgcgaccta tgacctcacc aactgggcaa ccttgaagga
     4381 ctggccacca atcgtagcgc tcgttgatat ggtgtggggt acggtgctct ctgcatcggt
     4441 tgctgggcta agctacctgc ttgctgtgac cttcgtagtg aaataactat gaccattctt
     4501 ctcacggcgc tcgccgtcct cctcatctac atgagtgggc ttttcgtaat tgcgactctc
     4561 gcgaagaata atggcgtcgc agacataggg tacggcatcg cattcattgt gctcatcggg
     4621 accacgctca ttgtttcttc gagcgtttct ttctatgtgc tcctcctctc attcctcccg
     4681 ctcgtctggg gagtacggct cgcgacgcgt atctatcgga agaacaaagg gaaaccagaa
     4741 gacttccgat ataaggcgtg gcgtgatgcg tggggcacct cattcctatt tcgaagtttc
     4801 cttcaggtgt atatgcttca agggctcgtg gtcttcgtgg tcgcgcttcc cgtgacgctt
     4861 gctattgcgt atccgacact cgtagtaatg ccggcactcg tatgggtagg tgtcgcactc
     4921 tggtgcgttg ggttcttttt cgaagcggta ggagatgcgc agctcgacgc ctttctcaag
     4981 aatcctgtaa acaagggaaa gataatgacg agcggactct ggcgctactc gcgtcatcct
     5041 aactatttcg gtgagagtct catgtggtgg ggtatcgcgc ttgcgtgctc tgctctttcc
     5101 tccgttccgc tcgtcgggtt catcggcccc attctcatca ccttcctttt attaaaagtc
     5161 tctggggtac cgcttctcga gaagcacttc gacgggaatc cggaatggga agcgtataaa
     5221 gcgcggaccc cagtgtttat tccactcccc ccgaaggtat aaaaaacagc gcag
//
LOCUS       DAORIN010000040         5070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_3237_length_5070_cov_3.376869, whole genome shotgun sequence.
ACCESSION   DAORIN010000040 DAORIN010000000
VERSION     DAORIN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3237_length_5070_cov_3.376869"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="PK109_02170"
     CDS             <1..1394
                     /locus_tag="PK109_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24373.1"
                     /translation="LFGILRASLQVSGLSKLKATATTIASSQMEYIRSLSYTAIGTDG
                     GIPAGAIAQTATTTNGGLPFTVRTYIEYADDAKDGVGAADTNGITTDYKHIKVTVSYT
                     VAGVTRSVTLVSNAAPPGLETTTGGGTLSANIVNAVGTAVTGATVRIVNASTSPDIDL
                     TTFSDSYGQVILPGAPTSTQYQISVTKTGYSTATTYARTATNANPSPGYLTVVGGSTT
                     SSTFAIDLLGTLILRTFSPIAAALWTDLFTDTSKVVSFSNTQVIGGALTLSGGVGTYP
                     ASGTALSTTTAPTYLAQWTNASSTISLPVGTTALVSVTDASGTLLPDAALPGNAAGFS
                     GTITLSSVSTTTYPALKLRATLTSSDPNVTPSISDWRIGYDEGPLPLPNVSFTLTSSK
                     TIGSTSGGASIYKTTIATTTDSSGVRTLSLEWDLYQLSIPGKTIVTASPDDPPIELLP
                     GATVDERLILTTP"
     gene            1391..1975
                     /locus_tag="PK109_02175"
     CDS             1391..1975
                     /locus_tag="PK109_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24374.1"
                     /translation="MRGISLVETVVVIAITAMVAGSLLSAIRYFYVNNAYVFEAASSV
                     DSARRGIATALSDLREASYADDGAYPLGSVATSSITFYSDVDADGGVERVKIWRVSNT
                     LYKVVTNASGNPPSYTGGGSSTTTIATYLRNATSTPLFAYYDANGTKLSTTSTPIASI
                     ATVAMTIQIDLNPNRAPNVFTLTGSATMRNLRDE"
     gene            1977..3419
                     /locus_tag="PK109_02180"
     CDS             1977..3419
                     /locus_tag="PK109_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24375.1"
                     /translation="MKTPHRGYLMLLALVFGAVFLTVFGGLSMYVLSENRLQSHNASS
                     AKAFSLAESGLEYYRWFLAHYPNDVQNGTGIAGPYVISYDDPEGGVAGTVTLTINGNT
                     ACGQTTSVDIQSVGRPSDDTSTTRTLRARYARPSVGSYSYILNDSVWAGDDRVILGPY
                     HSNGGIRMDGTANSPVTSSLSTWNCNSNYGCSPTQSNAPGVVGDGPNQTLWSYPQPQV
                     DFSAISADFGSLKTLATASGVYYPRYSTGTATTSASYWNGYHITFNSNGTMTVRRVSA
                     TTQLQVVPVNSAEWNTDRALIATEAAYETRTIPSTCGLIFVEDNVWVDGTIPSKVTLV
                     AANIVNANVAPNVYLRDNLQYGAYDGTDGLTLVAENDVLVAPNAPTTMTLNGIFIAQG
                     GAFGRNLYLNSGRSDCHSTYEPKTSLTILGTTVSNKRTGTKWMNGCGSGSDAGFQTRI
                     DSYDRRIATDPPPFTPVVSTDYQFVDWREE"
     gene            complement(3420..3998)
                     /locus_tag="PK109_02185"
     CDS             complement(3420..3998)
                     /locus_tag="PK109_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24376.1"
                     /translation="MSDSIPTKIRSFISSHKKLTYWVGGGILVILILIVGLFATANFV
                     VTSTPERSDDVASFMHRFETAIPLVPLGGTPEEVAAAIDANYTPFVSPELLAAWKADP
                     ANALGDPAGDATYAGMRVKSIRNVGVLSYIVKAYLQERTPVTIPGLGSGTAKRDVPVT
                     FGVSWKGNSWKITEYALGHGTDDEAATTTPVQ"
     gene            4077..4466
                     /locus_tag="PK109_02190"
     CDS             4077..4466
                     /locus_tag="PK109_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24377.1"
                     /translation="MEPTLLLARVFGVYFIIAAVMVFMNRKALMISVEAMFKERFAQM
                     IAAMLSILGGLLFINLYQDWSTLASGIISAIAWLILLKGLLYAFVPEARLVKLFKGFT
                     EGNWYTMDGILALVLGIYLSGIGYGMW"
     gene            4486..>5070
                     /locus_tag="PK109_02195"
     CDS             4486..>5070
                     /locus_tag="PK109_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRH24378.1"
                     /translation="MLVVANWKSYVETREEAKKLFALAKRLASSVRRVRIVLAPSTPH
                     LGLLAPGNRSKVAVAAQDISDTTVAAATGEVSGSTLRNIGATYVIVGHSERRARGETD
                     AVVVEKVRRALVNGLTPILCVGERERDADAQYLVLLKSQISSVFSLLSPKERLDVILA
                     YEPVWAIGKHAGDGPSPSDIAEMVLYIRKVLSEYL"
BASE COUNT         1120 a         1461 c         1332 g         1157 t
ORIGIN      
        1 gactcttcgg catactgcgc gcatcgcttc aggtatccgg tctttcaaaa ctgaaggcca
       61 cggcaacgac gatagcgagt tcgcagatgg aatacattcg ctccctctcg tataccgcta
      121 tcggcaccga cggcggtatt cctgcaggcg ccattgcaca gacagcaacg actacgaacg
      181 gcggtcttcc gtttaccgtg cgtacctata ttgaatacgc ggatgacgct aaggatgggg
      241 taggggcggc agacacgaac ggcatcacga ctgactataa gcacataaag gtgacggtct
      301 cctacacggt tgccggcgtc acgcgctcgg tgaccctcgt atcaaatgca gcgcctcccg
      361 ggcttgagac caccacgggc ggcggtaccc tctctgcaaa catcgtaaac gcggtgggaa
      421 cggcagttac gggcgctacg gtacgcatcg tgaacgcttc cacctctcct gacatcgatc
      481 tcacgacgtt ctctgattcc tatgggcagg tgattctccc gggcgcgcct acctcaaccc
      541 agtaccagat atcagtgacg aagaccgggt actcaaccgc tactacctat gcacgtaccg
      601 ccacgaacgc gaacccgtcc ccgggatatc taaccgtggt gggtggctca acgacctcga
      661 gcacctttgc cattgatctc ctcggtacct taattcttcg taccttctct cctattgctg
      721 ctgctctatg gacggatctc tttacggata cttctaaagt agtttcgttc tcaaacacgc
      781 aggtgatagg aggagcatta acgctctcgg gaggagtagg aacctatccg gcaagcggta
      841 cggcgctttc tacgaccacg gcgcccactt atcttgcgca gtggactaac gcatcttcta
      901 ctatatcgct tccagtgggc acgacggctc tcgtgtcagt cacggacgcg agtggcacct
      961 tacttcctga cgcagcgctt ccggggaatg cggccggatt ctcaggcacc atcacactct
     1021 caagcgtttc aacgaccacg tatccggcgc ttaagcttcg tgccacgctc acttcatcag
     1081 acccgaacgt aactccaagc ataagtgatt ggcgtatcgg atacgacgaa ggaccgctcc
     1141 cgctcccgaa cgtttctttc acgctcacga gctcgaagac catcggctca acgtccggcg
     1201 gcgcttctat ctataagacg acgatagcga cgactaccga tagttcgggc gtgcgcaccc
     1261 tatcgcttga atgggacctg taccagcttt ccattcctgg gaaaaccatt gttaccgcat
     1321 cgcccgacga tccgcctatt gaacttcttc ccggagcaac cgttgatgag cgcctcatcc
     1381 tcaccacgcc atgagaggga taagcttagt tgagacggtc gtcgttatcg ccatcacggc
     1441 gatggtcgcg ggctcgctcc tttccgccat ccggtacttc tatgtaaaca acgcgtatgt
     1501 gtttgaagcg gcgtcttccg ttgatagcgc gcgtcgcggt attgcgaccg cgctctctga
     1561 tcttcgcgaa gcgtcctatg ccgatgatgg ggcatatccg cttggaagcg tcgcaacctc
     1621 aagtatcacg ttctattcag acgttgatgc cgatggcggt gtggagcgcg tgaagatatg
     1681 gcgcgtcagt aacacacttt ataaggtggt cacgaacgcc tcaggcaacc cgccttccta
     1741 caccggcggg ggaagtagta cgacgaccat cgctacgtat cttcgtaacg ccacctcaac
     1801 gcccctcttc gcgtattatg atgcaaatgg cacaaaactt tcgactacct caactcctat
     1861 cgcttcaatt gctaccgttg ccatgactat tcaaatcgac ttgaacccga accgtgcacc
     1921 aaacgtattt acgctcaccg gaagcgcgac gatgcgtaac ttgcgcgatg aataatatga
     1981 agactccgca ccgtggctat ctgatgttac ttgcgctcgt gtttggcgcg gtgttcctca
     2041 cggtattcgg cggcctttcc atgtatgtac tctcagagaa ccgtctccag tcgcataacg
     2101 catcgtccgc caaagcattc tctctcgcag aatctggcct tgagtactat cgctggtttc
     2161 tcgcgcacta tccgaacgat gtgcagaacg gcacggggat tgccgggccc tatgtcatta
     2221 gttatgacga cccagaaggt ggggttgcgg gtacggtgac gcttaccatt aacggcaata
     2281 ccgcctgcgg acagactacc tcggtagata ttcagtcggt gggccgtccg agcgatgata
     2341 cgagcacgac gcgcacgctc cgtgcgcgct acgcacgtcc ttcggtcggg agctactcct
     2401 atatattgaa cgattcggtg tgggcaggcg atgaccgcgt cattctgggc ccgtaccata
     2461 gtaacggcgg catacgcatg gacggtacgg caaactcccc ggtcaccagc tctctctcta
     2521 cctggaactg caacagtaac tacggatgct cacccacaca atcgaacgcg ccaggcgtcg
     2581 taggagacgg accgaatcaa accctgtgga gctatccgca gccgcaagtg gacttcagtg
     2641 ctatctccgc tgacttcggt tccctgaaga ccctcgctac cgcaagtggc gtctactacc
     2701 cccgctactc tactggcacc gcgaccacct ccgcttccta ttggaacgga tatcacatca
     2761 cgtttaacag taacggtacg atgacggtgc gtcgcgtgtc ggcaacgacg cagcttcagg
     2821 tggtgccagt gaactctgct gagtggaaca ctgaccgcgc actcattgca acggaagccg
     2881 cgtatgagac gcgcaccatt ccttccacct gtggtcttat tttcgttgaa gacaatgtgt
     2941 gggtggacgg cactattcct tcaaaggtga cgctcgttgc cgcaaacatc gtgaacgcga
     3001 acgtcgcacc aaacgtgtat ttgcgcgaca acctccagta cggcgcgtat gacgggaccg
     3061 acggcctcac actcgtcgct gagaatgatg tgctcgtcgc gccaaacgcg cccaccacca
     3121 tgactctaaa cggcatcttc atcgcacagg ggggcgcctt cggacgtaat ctatacctaa
     3181 atagcggccg cagcgattgt cattcgacgt atgaaccaaa aacatccctt accattcttg
     3241 gcaccaccgt ttcaaacaag cgcacgggaa cgaaatggat gaatggctgt gggtcgggaa
     3301 gtgacgctgg gttccagacc cgcatcgact cctatgatcg gcgcattgct accgacccgc
     3361 ccccctttac gcccgtagtg tctacggact atcagttcgt tgactggaga gaggagtagc
     3421 tactgaaccg gcgtagtcgt agctgcttcg tcatcagtgc cgtgaccgag tgcgtattcc
     3481 gttatcttcc aggagtttcc tttccatgag acgccgaagg tgacgggtac gtcgcgcttt
     3541 gctgttccgc ttccaaggcc aggaatagtc actggtgtgc gttcctgaag atacgctttc
     3601 acgatgtagc tcagcacgcc cacattgcgt attgatttta cgcgcatacc tgcgtacgta
     3661 gcgtcgcccg cagggtcgcc aagcgcattc gcaggatctg ctttccatgc ggccaagagt
     3721 tcaggagaaa caaatggcgt gtagttagcg tctattgctg ccgccacttc ttcaggggtg
     3781 cctcctaaag gaaccagggg aatagccgtt tcaaaccggt gcatgaatga ggcgacgtcg
     3841 tcagagcgct cgggggtgga ggtgacgaca aagttagctg tcgcgaagag acctactata
     3901 aggataagaa tgacgaggat accgcctcct acccagtagg taagcttttt atgagaagaa
     3961 ataaacgaac gaatcttggt tgggatggaa tcagacatag tggtgtatag aaccaagatt
     4021 accatggagg ccatatgaag gctctagggg gaaatacgtg ggttataata ataggtatgg
     4081 aaccaacact actgctcgcc cgcgtattcg gtgtctactt tattattgct gctgtcatgg
     4141 tctttatgaa ccgtaaggcg ctcatgataa gcgttgaagc catgttcaaa gagcgctttg
     4201 cgcagatgat tgccgccatg ctttcaattc tcggagggtt gctctttatc aacctctatc
     4261 aggactggtc cacgctcgcg tccggcatta tctcggccat tgcctggctc attctcttga
     4321 agggccttct ctacgcgttt gtccctgaag cgcgtctcgt gaagctattc aaagggttta
     4381 cggaaggaaa ctggtacacc atggacggta tccttgcgct cgtactcggt atctaccttt
     4441 ctggcatagg gtacgggatg tggtagcgcc tgctatcata gggagatgtt agtcgtcgcc
     4501 aactggaaat cctacgtaga gacgcgtgag gaagcgaaaa aactcttcgc actcgccaag
     4561 cgccttgcta gttccgtacg ccgggtccgt attgtgctcg ccccatcaac gccgcacttg
     4621 ggacttctcg ctcccgggaa ccgttctaaa gtagcggttg ccgcacaaga tatttccgac
     4681 acgacagtag ccgctgctac cggcgaagta tcgggcagta cgctacgcaa cataggtgct
     4741 acctacgtca tagtcgggca ctcagaacga cgcgcgcgcg gagaaaccga tgcggtagta
     4801 gttgaaaagg tacgacgtgc tctcgtgaac ggtctgactc ctattctctg cgttggggag
     4861 cgggaacgcg acgctgacgc gcagtatctg gtcttactca agtcgcaaat cagctcggtc
     4921 ttctctctcc tttctcccaa agaacgtctc gacgtcattc tggcgtacga accggtgtgg
     4981 gccattggca agcatgccgg cgacgggcca tcgccctcgg acatcgccga aatggtgctc
     5041 tacatacgaa aagtgctgag cgaatatttg
//
LOCUS       DAORIN010000041         5004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_3303_length_5004_cov_5.176803, whole genome shotgun sequence.
ACCESSION   DAORIN010000041 DAORIN010000000
VERSION     DAORIN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3303_length_5004_cov_5.176803"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PK109_02200"
     CDS             complement(<1..617)
                     /locus_tag="PK109_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRH24379.1"
                     /translation="MQRDYPLEKVRNFGIVAHVDAGKTTTSERVLFYTGSQHKIGEVH
                     EGETTTDWMEQERERGITITAAAITCFWTRTSEKDKKDPAKKFRFNVIDTPGHIDFTA
                     EVKRSMRVLDGAIVVFDGVAGVEPQSETNWRYADEAKVPRLCFINKLDRTGASFEDSY
                     KSILDRLSKKAVRAQIPMGAEGDFAGVVDLLTMKAYTFDGNMGENV"
     gene            1034..1336
                     /locus_tag="PK109_02205"
     CDS             1034..1336
                     /locus_tag="PK109_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24380.1"
                     /translation="MAETDLMKIGFKKLPHLSLNYGARMKGDEKQIQLTAYDEDDNAL
                     PAVTFFRDQLPFYAIFDEETKKITIVVMSEKNVLYKKAIVYASKHGIPRRLHHHKV"
     gene            1470..1787
                     /locus_tag="PK109_02210"
     CDS             1470..1787
                     /locus_tag="PK109_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24381.1"
                     /translation="MFDTCVAHGLLPVTSYTVTDGEVESLGGAPIFVSVYTINDENGP
                     LRFRVSKGYELPLFVEQRVPSFQPALLVLHDKERMHYRRGLALSLRNALNEARYRHHF
                     DAW"
     gene            1859..2137
                     /locus_tag="PK109_02215"
     CDS             1859..2137
                     /locus_tag="PK109_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24382.1"
                     /translation="MEHEPTAEKLPSRVLGEMGFTCNFYDDPDVAPKSFDTVDRGDEV
                     VVMAYDEKRVSWTLTSTESVETLETMMKEKGFTPNPSLLLPTEEDPIN"
     gene            complement(2179..2676)
                     /gene="rpsG"
                     /locus_tag="PK109_02220"
     CDS             complement(2179..2676)
                     /gene="rpsG"
                     /locus_tag="PK109_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRH24383.1"
                     /translation="MRHPVKNKHPRTPDAEYKSEAIARFINTVMLDGKKDTARAVVYD
                     ALELLKKHTKETKEGEEGGIATPLEIFETAIRNVSPLMEVRSRRVGGANYQVPREVPQ
                     QRRVSLAYRWLINAARAKKGKPMAEKLAEEILLATKNEGAAIKKKDEMHRMAEANKAF
                     AHFAW"
     gene            complement(2680..3090)
                     /gene="rpsL"
                     /locus_tag="PK109_02225"
     CDS             complement(2680..3090)
                     /gene="rpsL"
                     /locus_tag="PK109_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRH24384.1"
                     /translation="MPTIQQLSKKRRKDKTRKSKVLALGRGFNHLENRPTFYPSPFKR
                     GVCVKVTTKTPRKPNSAIRKIARVRLTNGMEVTAYIPGEGHNLQEHSVVMLRGGRVKD
                     IGVQYTIVRGRLDTAGIDKRRRGRSRYGAKKPKA"
     gene            3287..3727
                     /locus_tag="PK109_02230"
     CDS             3287..3727
                     /locus_tag="PK109_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24385.1"
                     /translation="MGPESPTPPPEGAPEAPHRSIDKMARLFLAAMNEVAQSFPDEFT
                     ARSRQTLLTERHLVKVKYNINKCMREDAAQAKDEGVAYERSKGERETKRSQIPYLYPG
                     NKQELFLTYAAHALGKVASPADRLPESFINALIQEMKLRIQKPD"
     gene            complement(3739..4725)
                     /locus_tag="PK109_02235"
     CDS             complement(3739..4725)
                     /locus_tag="PK109_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRH24386.1"
                     /translation="MSIPGNLPGQHRPHPARHLFSIAGISIFTVLIIGSFFMAAVYLR
                     TLIETNQLAAVITSTLVDLANDDRKQDNLGTLTINPKLVAAAQAKANDMAAKGYFAHN
                     SPDGQTSWTWFQQAGYSFSYAGENLAVNFADSEDVEEAWMDSPTHRANILNGKFTEIG
                     IATAQGEYKGKKTTFVVQMFGRPKQTTAFAPVNESTQPENPEEIAIATTETGGTVVLG
                     TAIPAPAAATSAPVAAVEEVPAQKAPTYSSMFGFLVSSPQNLLRTIYVFSALIVILAL
                     GLTTRMEFKRHHFRHMVAAVFLLALMGGLFTAADHLVFKPPVIGMTQVSVVG"
     gene            complement(4762..>5004)
                     /locus_tag="PK109_02240"
     CDS             complement(4762..>5004)
                     /locus_tag="PK109_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24387.1"
                     /translation="ATTTISVTGEAVSQLTVGTIVYAKIPAAKVATFRILIETRGKIG
                     NEYNVLITQINYKQDPSDARYGKFVEALPSKSVRFT"
BASE COUNT         1197 a         1282 c         1317 g         1208 t
ORIGIN      
        1 acgttctcac ccatgtttcc gtcgaaggtg taggccttca tagtgaggag gtcaacgacg
       61 cctgcaaagt cgccttcagc acccataggg atctgtgcac gaacggcctt ctttgaaaga
      121 cgatcaagga tggacttgta ggagtcctca aacgatgctc ccgtgcgatc aagcttgttg
      181 atgaagcaga ggcgcggcac ttttgcttcg tcagcgtagc gccagttggt ctccgactgt
      241 ggttctactc ctgccacgcc gtcgaacacg acgatagcgc cgtccaagac acgcatcgag
      301 cgcttcacct cggcagtgaa gtcgatgtgt cctggggtat caataacgtt gaaacggaac
      361 ttcttcgcag gatccttctt gtctttctcg ctggtgcggg tccagaagca ggtaatagca
      421 gcagccgtaa tggtgatgcc gcgttcacgc tcctgttcca tccagtcggt ggtggtctca
      481 ccttcgtgta cttcgccgat cttgtgctgg gaaccggtgt agaagagcac acgttcagac
      541 gtggtcgtct ttccggcgtc cacgtgggcg acgatgccga agttacgtac tttctctagg
      601 ggataatcgc gttgcatagg gagtgaatat aaataaaaag ccgccgaggc gttacctaca
      661 agatactgaa aataccctga aaaagcaaag aaccacactc ctcccctgcc atgtcggcga
      721 tatgctattc tcccccacgg aggaggttcc catgaatgct cttatggaac acatggctct
      781 tactgggctc ggattatccg aatctagaaa agagaccgat agtactcgtg ctatcgaaag
      841 aggtacgtac cgggtagtcg gcaatcccca tgcgcatatc tacttacgcg accactacgg
      901 cacgctctgg cacggtcccc ataccaccca ggtggagaac tatctcaaat aagactaagg
      961 acggcgcccc aaagcgccgt tttccttatg aaagtggtat tgtttccgac aagccaagtg
     1021 gaggaatata ccaatggccg aaacagattt gatgaaaatc gggtttaaga agttgccgca
     1081 cctaagcttg aattacggtg cacgaatgaa gggtgatgaa aaacaaatac agctaacggc
     1141 gtacgacgaa gacgacaatg cacttcccgc tgtgacgttc ttcagagatc aactaccgtt
     1201 ctatgccatt ttcgacgagg aaaccaaaaa gataactatt gtcgtgatgt cagaaaagaa
     1261 cgttctctat aagaaagcga tagtgtacgc ctcaaagcac ggtattccgc gacgccttca
     1321 ccatcacaag gtctgagcac ccctcacatc aatactgaag cggcgtttcc ctcatgggga
     1381 ggcgccgttt tctttataca ccctcggtga tagggtgatc acagagcatg ttcccgctct
     1441 gacagatcat gtggagatac tgcatggata tgtttgatac gtgtgtggct catggcctcc
     1501 taccggtaac ctcatatacg gtgacggatg gagaggtgga gagtcttggt ggtgcaccga
     1561 tatttgtttc ggtatatacc atcaacgatg aaaacggccc gcttcgtttc cgcgtcagca
     1621 aaggttacga attaccgctt ttcgtagagc agcgcgttcc tagttttcag ccggcactgt
     1681 tagtgctgca cgataaagaa cgcatgcact accgccgcgg cctcgcactg agtttgcgca
     1741 acgcactaaa cgaagcccga tatcgtcatc acttcgatgc atggtgaccc tcacgtgcac
     1801 caagaaagaa acggcggctc ctaggagtcg ccgttgttct ttacgctata ctttacgcat
     1861 ggaacacgaa cctaccgctg agaaactccc ttcgcgtgtt ctaggggaga tgggatttac
     1921 ctgcaatttc tacgatgacc cggacgtagc accaaagtca ttcgatacgg tagatagagg
     1981 ggacgaagta gtggtcatgg cgtatgacga aaaacgagta tcctggaccc ttaccagtac
     2041 cgagagcgtt gaaacgcttg aaaccatgat gaaagaaaaa gggtttactc caaacccttc
     2101 cctactcctt ccgacggaag aagaccctat taattgaaaa accgccccat acggggcggt
     2161 ttttcatttg cgtggatgtt accaggcgaa gtgagcgaat gccttgttgg cttctgccat
     2221 acgatgcatt tcgtccttct tcttgattgc tgcaccttcg ttcttggttg caaggaggat
     2281 ttcctcagca agcttctcag ccattggctt tcccttcttt gcacgagcgg cattgatgag
     2341 ccagcggtag gccaaagaga cgcggcgctg ctgtggcact tcgcgtggca cctgatagtt
     2401 ggcaccaccg acgcggcgag agcggacttc catcaaagga gagacgttcc ttatagcggt
     2461 ctcgaagatc tcgagtgggg tcgcgatacc tccctcttca ccttctttgg tttccttcgt
     2521 gtgcttcttc aaaagctcga gggcgtcgta caccacggcg cgcgccgtat ccttcttacc
     2581 atcaagcatg acggtgttga tgaaacgagc gatagcttct gacttgtact cagcatccgg
     2641 ggtacgagga tgcttgttct ttactgggtg tcgcataggt taggccttag gcttcttagc
     2701 accgtaacga gagcgaccac ggcggcgctt atcaatacca gcggtatcga ggcgtccgcg
     2761 gacgatggtg tactgaacac cgatgtcctt cacgcggcca ccgcggagca tgaccaccga
     2821 gtgctcctgg aggttatggc cttctcctgg gatgtaggcg gtcacttcca tgccgttggt
     2881 gaggcgcacg cgggcaatct tacgaatagc ggagttcggc ttgcgcgggg tcttggtggt
     2941 taccttgacg cacacgccac gcttgaacgg ggatgggtaa aaggtcgggc ggttctcgag
     3001 gtggttgaag ccacggccga gggcgaggac ttttgacttc ctcgtcttat ccttgcgacg
     3061 cttcttgctg agctgctgaa tagttggcat tagtaagtga ttgtacgcac gtaataagtg
     3121 acttcttcct acgaatactg ctcacggtag taagcttcct tctccatagg tcctgctgag
     3181 gggtacttcc cctagcaaaa atcccgccgt gcgggacttc cacaatatat aggagagagt
     3241 acgagaaagc aattaaagcc attcggagcc gtatatactt atcattatgg gtccagaatc
     3301 ccctacccca cctcctgaag gagcacctga agctcctcat cggtctatcg ataagatggc
     3361 acgccttttc cttgctgcga tgaatgaggt ggctcaatca ttccccgacg aattcactgc
     3421 tcgaagccgt caaacacttc tcactgaacg ccatctggtt aaagtgaaat acaatataaa
     3481 taaatgcatg agggaagatg cggctcaagc gaaagacgaa ggagtggcat acgaacgtag
     3541 taagggcgag cgagaaacaa aaagatcaca aatcccctat ctctatccgg ggaataagca
     3601 ggaactgttt cttacgtatg ctgcgcacgc gcttggaaaa gtcgccagtc ccgcagaccg
     3661 tcttccggaa tcgtttatta atgcactcat tcaagagatg aaactacgaa tacagaagcc
     3721 tgattgacct cccccttctt atccaactac cgatacctgc gtcatgccga tgaccggtgg
     3781 tttgaagact aagtgatcag cggcagtgaa gaggcctccc ataagcgcaa ggaggaatac
     3841 cgctgccacc atatggcgga agtgatgacg tttgaactcc atacgagtcg taagcccgag
     3901 cgcaagaatc acgatgagtg ctgagaatac gtaaatggtg cggagcaggt tctgcggtga
     3961 ggacacgagg aatccgaaca tagatgagta ggttggtgct ttctgagctg gcacttcttc
     4021 tacagcagct actggggctg aggttgccgc cgcaggcgct ggaatagccg taccgagcac
     4081 tactgtgccg cccgtttccg tagtggctat ggcaatctct tcgggatttt ctggttgcgt
     4141 cgactcattc accggagcaa aggccgtagt ttgcttcgga cgaccaaaca tctgcaccac
     4201 gaaggtagtc ttctttccct tgtactcccc ttgtgcggta gcgatgccaa tttccgtaaa
     4261 ctttccgttc aaaatgttcg cgcggtgcgt cggtgaatcc atccaggctt cctcaacatc
     4321 ttctgagtca gcgaagttaa cggcaaggtt ctcgcctgca tagctgaagg agtagcctgc
     4381 ctgctgaaac caggtccacg aggtctgtcc gtcgggagag ttgtgcgcaa agtacccttt
     4441 tgccgccata tcattcgctt ttgcttgcgc cgcggcgacg agcttcgggt taatagtgag
     4501 tgtcccgagg ttatcctgct tgcggtcatc gtttgcaaga tcaactagcg ttgaggtgat
     4561 aacggcagcc agctggttgg tttcaataag ggtacgcagg tacaccgcag ccatgaagaa
     4621 gcttccaata ataagaacgg tgaagatgga aatgcccgca atactgaata ggtgacgcgc
     4681 cggatgggga cggtgttgac cgggaagatt tcctggtatt gacatacgag aaatatacca
     4741 cctaactctt actctgtcag cctaggtaaa gcgcacgctc ttactgggga gcgcttcaac
     4801 gaatttccca tagcgggcgt cgctcggatc ctgcttatag tttatctggg tgatgagtac
     4861 gttgtattca ttccctatct tccctctcgt ctctataaga atgcggaagg tagctacttt
     4921 tgcagcggga atctttgcat acacgatagt gcctacggtg agttgcgata ctgcctctcc
     4981 ggtaacggag atagtggttg tcgc
//
LOCUS       DAORIN010000042         4928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_3387_length_4928_cov_5.275600, whole genome shotgun sequence.
ACCESSION   DAORIN010000042 DAORIN010000000
VERSION     DAORIN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3387_length_4928_cov_5.275600"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..768
                     /locus_tag="PK109_02245"
     CDS             208..768
                     /locus_tag="PK109_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRH24388.1"
                     /translation="MTILYIVLALVVIVLVWFVLAYNGFVTLVNRAKEAWSDIEVQMK
                     RRYDLIPNLVETVKGYAAHESSAFENVTKARAAAMGATGVEGHAQAENMLTGALKSLF
                     AVAEAYPDLKANQNFLQLQAELGNTEDKIQAARRFYNGTVMSLNTGLEQFPGNIVGGV
                     FGFTKREFFELAEGEEAARQPVKVAF"
     gene            768..1133
                     /locus_tag="PK109_02250"
     CDS             768..1133
                     /locus_tag="PK109_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRH24389.1"
                     /translation="MSGKVFYNKLIRDLIPEKIAKAGALCETRVLDETEYEKELLKKV
                     EEEGSGVAAATTREELIEELADVQDVIDEIKKLKNITEEEVRAAQAVALKKKGGFSKR
                     LFLLWSSDDGYTTNEKNHG"
     gene            1126..2010
                     /locus_tag="PK109_02255"
     CDS             1126..2010
                     /locus_tag="PK109_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRH24390.1"
                     /translation="MANLYQERSENLFKTWGLMLGFLLVVMAVGWAIAGYYGDPSILY
                     IAVAFALFANVGSYWFSDKLVLSMTGAKPATREEYFDFYTVTENLAITAGLPLPKLYV
                     INDPAPNAFATGRDEKHAVVAATTGLLSMMNRQELEGVIAHELSHVKNRDMLVMTIAV
                     VLAGFVAIVADIFLRVSMYGGDRDNNKGGALFAILAVVAVVLAPIAATLIKLAVSRRR
                     EYLADASGALLTRYPEGLASALAKLGSYQGPMKSANHATAHLFIANPFGKSAGKFVNG
                     LFATHPPIEDRIKRLTGN"
     gene            2728..2815
                     /locus_tag="PK109_02260"
     tRNA            2728..2815
                     /locus_tag="PK109_02260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2762..2764,aa:Ser,seq:gga)
     gene            2815..3165
                     /locus_tag="PK109_02265"
     CDS             2815..3165
                     /locus_tag="PK109_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HRH24391.1"
                     /translation="MEYQGKVIGGSRRANALGFPTANIAFNDSTAHGVYAARVTLDDK
                     DYQSVAFADPARGVLEAHLFDFDGDLYGKDISITLIQKLRDSERFENDEDLKAAMVRD
                     AEQARSVRECKDVE"
     gene            3171..3803
                     /locus_tag="PK109_02270"
     CDS             3171..3803
                     /locus_tag="PK109_02270"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRH24392.1"
                     /translation="MFTGIIQKTVPITATSTVGKNVRLSVSKPAGWKLTKGQSIAVDG
                     VCLTVISQTSRTFTADAMPETLKKTTVGSFTPGRIVNLERALKLSDLLDGHLIQGHVD
                     SRAEVVSVVSHGTSKLITIRVPASLLKKIALHGSVAVNGTSLTVAKRVGNNISVALIP
                     YTLAHTNLGTLKKGDLVNLETDFLARHLTSGNRAKVKSNATRRIPKSTRN"
     gene            3766..4227
                     /gene="ribH"
                     /locus_tag="PK109_02275"
     CDS             3766..4227
                     /gene="ribH"
                     /locus_tag="PK109_02275"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRH24393.1"
                     /translation="MQRGEYQKARETKDGSALSVGLVVSSFNTDITGGMLEGALATLK
                     EWGVKEDRIHVQHVPGAYELPYGCQQLIDDKKPDAVIAIGCVIKGDTDHDRYIADAAA
                     NGLMRVMLDTKTPISFGVITTNNLEQAVIRSTGETNKGREAALAVLELLLP"
     gene            4224..4643
                     /locus_tag="PK109_02280"
     CDS             4224..4643
                     /locus_tag="PK109_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRH24394.1"
                     /translation="MRSLFLVLAIVVIIVGAYFYFQPKPTYALATIEVVATPSAREQG
                     LSGRTELAPDYGMLFVFPEDGDYAFWMKDMLVPIDIIWVTEMGTIAGIEHSVSPDTYP
                     TAFHPPYPVRYVLETRAGVAQEKGWSVGTELSLPIPR"
BASE COUNT         1240 a         1236 c         1304 g         1148 t
ORIGIN      
        1 gaaagtaacg cggggcgccg tagagaatgc ggtatccgca gcagcgatac tgctcactac
       61 cgaagctgcc gttgccgatc ttccagaacc aaagaccgct ccttctggag gggatatgga
      121 aggcatgggc ggaatgggat tctaatccag cgcactagcc ccgcgaaagc ggggtttttg
      181 ctatactaag gacataaccc ctcatatatg actatcctct atatcgtgct cgcactcgtc
      241 gttatcgtgc tcgtatggtt tgtgcttgcg tacaacggtt ttgtcacgct tgttaaccgc
      301 gctaaggaag catggtctga catcgaagta cagatgaagc gccgctatga cctcatcccg
      361 aacctcgttg aaacggtgaa gggatacgcc gctcatgagt cgagcgcgtt tgaaaacgtc
      421 accaaggcac gcgctgctgc tatgggtgca acgggtgttg aaggccacgc tcaagcagag
      481 aacatgctca ccggtgcatt gaagtcgctc ttcgccgttg cagaagcata tccagacctt
      541 aaggcgaacc agaacttcct ccagctccag gctgagcttg gaaacaccga agataagatt
      601 caggcagcgc gccgattcta taacggcacc gtcatgtctc ttaacacggg cctcgagcag
      661 ttcccgggga acattgttgg cggagtattc ggcttcacga aacgcgaatt cttcgagctt
      721 gctgaaggag aggaagcagc acgccagcca gtgaaagtcg cgttctaatg agcggcaagg
      781 tcttctacaa caaactcatt cgggatctca ttcccgagaa gattgcgaaa gcaggagcct
      841 tgtgtgagac gcgcgtatta gacgaaacgg agtacgagaa agaactcctg aagaaagttg
      901 aagaagaggg aagtggagtt gcggcagcga caacgcgcga agaactcatc gaagagttag
      961 cggatgtgca ggatgtcatc gatgagatta agaagttgaa gaacatcacc gaagaagaag
     1021 tgagggcggc acaagcggta gcactcaaga aaaagggagg attcagtaaa cgcctcttcc
     1081 ttctatggtc gtcagacgat gggtacacaa ctaacgaaaa gaaccatggc taatctctac
     1141 caagaacgtt cagagaactt gttcaagacc tggggcctca tgctcgggtt cttgcttgtg
     1201 gttatggcgg tagggtgggc aattgctggc tactatggtg atccgtccat tctctatata
     1261 gcagttgcgt tcgctctttt tgcgaacgta ggaagctact ggttctccga taagctcgtc
     1321 ttgtctatga cgggcgcaaa gcccgcaaca cgcgaagaat acttcgactt ctacaccgtt
     1381 accgagaact tagcgattac tgcaggactc ccgctcccca agctctatgt catcaatgat
     1441 cccgcaccaa acgcgtttgc taccggacgc gacgagaagc atgcggtagt tgcggcaacg
     1501 acggggcttc tttcgatgat gaaccgccag gaacttgaag gcgttattgc gcacgagctc
     1561 tcccatgtga agaaccgcga catgctcgta atgaccattg cggtggtgct tgcaggcttc
     1621 gtggcgatag tcgctgacat cttcctgcgt gtctcgatgt atggtggtga tcgtgataac
     1681 aataaaggag gcgcactctt cgctatactt gcggtggttg cggtcgttct cgcccctatc
     1741 gcagcaacgc tcatcaagct tgcggtctct cgtcgtcgcg aatatcttgc ggacgcgtcg
     1801 ggcgcacttc tgacgcgcta ccccgaaggt ctcgcctccg ccctcgcaaa gctcggcagc
     1861 tatcaaggac cgatgaagag tgctaatcat gcaaccgcac acctatttat tgctaatcca
     1921 ttcggtaagt ctgcagggaa gtttgtgaac ggtctcttcg ccacccatcc tcctattgaa
     1981 gaccgcatca agcggcttac tggaaactaa acttgtgtag gtgcggtaga aaagaccgcc
     2041 gttacgaaag caggtgctga gatactattc agatggtatc tgttgctagt cttaagtacc
     2101 caaagaaaag tcatcgtaaa aaggtgacgc caccaaagta ttctgctgct ctcgcggaat
     2161 tttttgggat aatgatgggg gatggtggta ttggtaatcc gtggcaggcg accataacat
     2221 taaattcgaa aaaggatctt tcatactcac gctatgtcag cgcgcttacg cagcggcttt
     2281 ttcacatcac tcctcaggta tttaaataca aaacgaaaaa agcgctcagg attttaatta
     2341 acagcgttac aattattgat ttcctggtat ctcaagggtt actacggggc gataagattg
     2401 cacaaggatt gagtattcct aagtggatat tagaaagtag tccttatcga cgtgcctgta
     2461 tccgtggtct cgtcgacact gatggatgcg cctacattca tacgcatgtt gttgctaaca
     2521 aagtgtataa aaatatcggg ttactttttt caagctattc tccgcacctt attgctgagg
     2581 tagctgcgat atttgaagaa ttcggcatta tgccgcatat tactactcga ggaacggata
     2641 tatacatata tcgagcagaa tcagtcgctc ggtatctgca agtatttggc tcttctaacc
     2701 cgcgtattac ttcagtttat gcggaatgga aacgtgtccg agcggtcgaa ggagcacgct
     2761 tggaaagcgt gtaaaccgaa aggtttcgag ggttcgaatc cctccgtttc cgccatggaa
     2821 tatcagggaa aagtaatagg cggaagccgt cgcgcaaacg cgctcgggtt cccaacggca
     2881 aatattgcgt ttaacgatag taccgctcat ggcgtgtatg ccgcgcgcgt cacgcttgat
     2941 gataaggact atcagtcggt tgcgttcgct gaccctgcgc gcggcgtgct cgaagcacac
     3001 ctctttgact ttgatggcga tctatacggg aaagatattt ccattacgct tatacaaaaa
     3061 ctgcgtgaca gcgaacgctt cgagaacgat gaggacctta aggcagcgat ggtgcgtgac
     3121 gcggaacaag cgcgtagcgt acgggagtgt aaggacgtag agtaatactc atgtttaccg
     3181 gcatcatcca aaagaccgta cctattactg ctaccagcac agtagggaag aacgtgcgtc
     3241 tttcggtgag taaacctgct ggctggaaat taacgaaagg tcagagcatc gcggtagacg
     3301 gcgtctgtct taccgtcatt tcccagacct cgcgcacctt tactgcagac gccatgccgg
     3361 aaactttgaa gaaaactacc gtcggatcat ttactcccgg acgcatcgtt aaccttgaac
     3421 gcgccttgaa actctctgat cttctcgatg gtcacctcat tcaagggcac gttgatagtc
     3481 gtgcagaggt agtgagcgtc gtgagtcacg gtacttcaaa actcatcacg atacgggtgc
     3541 ctgcgtcgct tctcaaaaaa atcgcactcc atggttcggt tgccgtaaac ggaacatcgc
     3601 tcacggtagc caaacgtgtc ggtaataata tttccgttgc gctcattccg tatacccttg
     3661 ctcatacgaa tctcggtacg ctaaaaaagg gagatctggt gaacctggag accgactttt
     3721 tggcccgcca cctgacgtcg ggcaatcgtg ctaaagtgaa gtccaatgca acgcggagaa
     3781 taccaaaaag cacgcgaaac taaggatggg agcgccctct cggtgggtct tgtcgtctct
     3841 tcctttaata cagatatcac cggcggcatg ctcgaaggtg ccctcgccac cctcaaggag
     3901 tggggagtga aagaagatcg aatccacgta caacatgtcc cgggcgctta cgagcttccg
     3961 tacgggtgcc agcagcttat cgatgacaag aagccggatg cggtcattgc tatcggctgc
     4021 gtcataaaag gtgacaccga tcatgaccgc tatatcgcgg atgctgccgc aaatggtctc
     4081 atgcgcgtca tgcttgatac aaagactcca atttcttttg gagtcattac caccaacaat
     4141 cttgaacagg ccgttattcg ttccaccgga gagactaata aagggaggga agcagcgctt
     4201 gcggtgcttg agttgttgct gccgtgagat cgctttttct cgtactcgcc atagtagtca
     4261 taattgttgg cgcgtatttt tattttcagc cgaaaccgac gtacgcactt gccactatcg
     4321 aagtggtcgc tacaccctca gcgcgcgaac agggattaag cggacggacc gaactggcgc
     4381 ccgactacgg catgctcttc gtgtttccgg aagacggtga ctatgcattc tggatgaaag
     4441 acatgctggt acctatcgat atcatctggg ttacggaaat gggaactatc gctggtatcg
     4501 agcattcggt ttcccccgat acctatccaa cggcgtttca tccgccgtac cccgtgcgct
     4561 atgtactcga gacgcgcgca ggcgttgcac aagaaaaagg atggtccgtc ggaactgagc
     4621 tctctcttcc tatacctcgt tagcgtggta taatctgaac ggaccggccc aggcgatcgc
     4681 tcctagctct gctaggggag gaaagtccga acaccggcta gcaatagcag caaggtagcg
     4741 ggtaacgccc gtccgccgca aggcgcgagg tgcgaacagt gacgtctgcg gcgaaagcca
     4801 acgagatcct tcgggattaa ttccaccgaa atgtggaaat gaaacggcaa aatccttacc
     4861 ccggtgcaag gccgtgccct cataagaggg agcgccgctc gagccggagg gtaacctacg
     4921 gcccagat
//
LOCUS       DAORIN010000043         4904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_3418_length_4904_cov_4.779748, whole genome shotgun sequence.
ACCESSION   DAORIN010000043 DAORIN010000000
VERSION     DAORIN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3418_length_4904_cov_4.779748"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..137)
                     /locus_tag="PK109_02285"
     tRNA            complement(51..137)
                     /locus_tag="PK109_02285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Leu,seq:taa)
     gene            complement(151..222)
                     /locus_tag="PK109_02290"
     tRNA            complement(151..222)
                     /locus_tag="PK109_02290"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Cys,seq:gca)
     gene            complement(236..568)
                     /locus_tag="PK109_02295"
     CDS             complement(236..568)
                     /locus_tag="PK109_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRH24395.1"
                     /translation="MSVRDEKAAERLVHLAADYIALEAGRETLITPLRAEISPDRKNA
                     TVYVSIFPDAERDHALEFLKRHKDLFRDFLKKEGRFAVLPFVRFEFDYGEQNRQHLDE
                     LSRDIELG"
     gene            complement(571..2046)
                     /locus_tag="PK109_02300"
     CDS             complement(571..2046)
                     /locus_tag="PK109_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRH24396.1"
                     /translation="MASTTRHPIVAVMGHIDHGKSSLLDYIRKSNVTAGEAGGITQHV
                     SAYVAEHEHEGTVRKITFLDTPGHEAFKALRARGAAAADIAILVIASDEGVKPQTKEA
                     YEEIMAAGIPFVVALTKIDKNGADPEKAKISALEAGIYLEGLGGSIPFTPVSSKTGEG
                     VPELLDLVLLTADLAELTGDASLPASGFVLESSADPKQGVSATLIVKNGTLTTGTVVV
                     AGASMAPVRFLEDFKGTRIKEAGPSEPVVVAGWSVLPSAGQLFTSVATKKEAEKLITA
                     PPVIKNTIAMQDPSGKTVLPVVLKADVTGSIEAITGLVDKLTHERVAVKILSSGIGSV
                     SENDVKTAAAAGGVIIAFHAATESSARELAERVNVPIESFTIIYELEKRIADLLIERA
                     PKMRTEEILGELKVLKAFSMSGNKQVLGAKFVSGMLSVNDQVKISRRGIDLAGGRILN
                     LQVARADVSEIHVEGEFGLQVESKTEIAGGDVLVAFRVVEN"
     gene            2106..3272
                     /locus_tag="PK109_02305"
     CDS             2106..3272
                     /locus_tag="PK109_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRH24397.1"
                     /translation="MGMRIYLDYAAAAPVSPGALKAYTKAVKAFGNPSAAHEEGRAGK
                     AILEDARKRIAHLAGAKPDAIVFTAGATEANALAIQGHVKALQERGVPLEKIHLLYLP
                     GAHASTDAAMTMLAKEGARVELLPIKDGDIDLTTLAGLLRPETALVSMEAVSSETGAR
                     FNTRGVRQVLDAHSGAPVLLHVDATQLPFAESFERTRLAADLISLDAQKVGGVRGIGC
                     LIVSPRTTLLPAIGGGGQERGLRSGTETPAAALAFAIALEEAAGAREAFTARALAARN
                     ACIATITSALPDAVVNEGKENVAHILNFSFPGRDTDFLAALLDERGIAVSTRSACETD
                     SVEGSRAVQALTGDSARATATLRISWGPVTKTDDLRRAMTALIEAVRFIDHTRL"
     gene            3290..4009
                     /locus_tag="PK109_02310"
     CDS             3290..4009
                     /locus_tag="PK109_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24398.1"
                     /translation="MDIEDLSKSQLLLLTILVNFVVSIATGVLTVSLLDEAPTTVTQT
                     VNRIVDHTIETVTTQVPTIGKDSTPSSEELLTAAIAENAEHMVTISYRVGAESTVVNG
                     LYLPSTRLVMTASSNMPGHITVTFPNGAAVEADRTASDGTLMRYGFAADAVLPQASTL
                     TLVPASSLKQGQTIIGLSADGKAVTGIIGKVDTDGIHAELPAVSPGAPIVNLSGAVIG
                     IAAGGNLFYTADRISTLQAAP"
     gene            4040..>4904
                     /locus_tag="PK109_02315"
     CDS             4040..>4904
                     /locus_tag="PK109_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRH24399.1"
                     /translation="MPPFKNFTTKAKEAIRKAHELAIERGQNHVSPLHLLAALIHQEE
                     SLVFSVLDRMDVDTMLLADTLLENLEAPETSSTLSPSYQLYLTPDLAQSLEASGKVAA
                     RMNDAFVGTEHLFVAVLEHPGPAGDILSRFKIDADTVQLVLKELKNAKDGQVVEPKKF
                     RALAKYTRNLTKLASENALDPVIGRDQEIHRVIQILSRRTKNNPVLIGEAGVGKTAIA
                     EGLASRMANNEVPESLKNKELLSLDLGLMIAGTKYRGEFEERMKTVMKEVERAEGKVI
                     LFVDELHTLVGA"
BASE COUNT         1121 a         1367 c         1292 g         1124 t
ORIGIN      
        1 caaagaacgc caatacgaaa atccggaaaa atctgaacta gtttcttgtt tggtgccgag
       61 agtgggaatc gaacccacac gacatttctg tctggggatt ttaagtcccc tatgtctacc
      121 aattccatca tctcggcagt ggtgttcttg aggcccgagc gggaatcgca cccgcgcatg
      181 gaggatttgc agtccccagc gttactactt cgccatcggg ccgtacttga tgatattagc
      241 ctaattctat gtctcgagaa agctcatcaa gatgttggcg attctgttct ccgtagtcga
      301 actcgaagcg aacgaagggg agtacggcaa agcgtccttc cttctttaaa aagtctcgaa
      361 agagatcttt gtggcgcttc aggaattcaa gggcgtgatc gcgttcagcg tcagggaaga
      421 tactcacata taccgtagcg ttcttgcggt ctggtgatat ctcagcgcgg agcggagtga
      481 ttaaggtctc acggcccgct tcaagcgcaa tatagtcggc agccaggtgc acgaggcgct
      541 ctgctgcttt ctcgtcgcgt acgctcatag ttagttctct acgacgcgga aggcgacgag
      601 tacgtctcct cctgctattt cagtctttga ctctacctgg agcccgaatt cgccttcaac
      661 gtggatttct gagacgtcag cacgcgctac ctgaaggttc agaatacgtc ctccggcaag
      721 atcaataccg cgacgagaga tctttacctg atcgtttacc gagagcatgc cggacacaaa
      781 ctttgcgccg agcacctgct tattgccgct catagagaac gctttcaaga ccttgagttc
      841 accgagtatt tcctccgtgc gcatctttgg tgcacgctct atcaagaggt cagcaatgcg
      901 cttctcaagc tcatagataa tggtgaagct ctcaatagga acgttgacgc gttcagctag
      961 ctcgcgtgcc gatgattcgg tagctgcatg gaaggcgatg atgacgccgc cagcggctgc
     1021 tgcggtcttc acatcgttct ctgacacgga gccgataccg gaggaaagaa tcttcacggc
     1081 aacgcgctca tgcgtaagct tatccacgag tccggtaatc gcttcaatgc tgccggtcac
     1141 gtcggcctta agaacgacag ggagcaccgt ctttccggaa gggtcctgca tcgcaatggt
     1201 gtttttgata acaggaggag cagtgatgag tttctctgct tctttcttgg tcgcaacgct
     1261 cgtaaagagc tgacctgctg acggcaggac gctccagcct gctacaacta ccggctcaga
     1321 cggtccagct tctttgatgc gagttccctt gaagtcttca aggaaacgta cgggcgccat
     1381 agaggctccg gcaacgacta cggttccggt agtgagcgta ccgttcttaa cgataagggt
     1441 tgcagacacg ccttgctttg gatcggcaga ggactcgagc acaaagcctg aggcgggaag
     1501 tgaagcgtct cctgtgagtt ctgcaagatc agcggtgaga agcacgaggt ccagaagctc
     1561 aggaacgcct tcaccggttt tggaggagac gggagtaaag ggaatagaac caccaaggcc
     1621 ttcaagatag atgcctgctt caagtgctga gatcttcgct ttctccgggt cagcaccgtt
     1681 cttatcaatc ttggtgagag cgaccacaaa cggaatgccg gctgccatta tttcttcgta
     1741 tgcttccttg gtttgcggct tcacgccttc atcgcttgcg ataaccagaa tagcgatatc
     1801 tgccgctgca gcaccgcgcg ctcgaagcgc tttaaatgcc tcgtgtcctg gagtgtcgag
     1861 gaaggtgatt ttacgcaccg ttccttcgtg ctcgtgctcg gctacgtagg cagagacgtg
     1921 ctgggtaata ccgcccgctt cgcctgcggt aacgttcgac ttacggatgt agtcgagaag
     1981 tgaagacttc ccgtggtcga tgtgacccat gacggccacg atagggtggc gagtggtaga
     2041 tgccataata tagtcagtac accacgcgcc ttccgtaaaa tcaattgagc gcgtatcgta
     2101 gtggtatggg aatgcgtatc tatctcgact atgcagctgc cgctccagtc tctcctgggg
     2161 cgttgaaagc gtacacaaaa gcggtgaaag cgtttgggaa tccgtctgcg gcgcacgagg
     2221 agggacgggc agggaaggca attctcgagg acgctcgtaa gcgcatcgcg cacctcgctg
     2281 gcgcaaagcc agacgctatc gtttttaccg caggcgctac cgaggcaaac gccctagcca
     2341 tacaagggca cgtgaaagca ctccaagagc gtggcgtgcc tttagagaag atccacctcc
     2401 tctatcttcc tggtgcgcac gcctctactg atgcggctat gaccatgctt gctaaggaag
     2461 gcgctcgggt tgagctactt cctataaaag atggcgacat tgatctcaca acacttgccg
     2521 gactacttcg tccagagacc gcactcgtgt ctatggaggc cgtttcttcg gagaccggtg
     2581 cccgctttaa tacacgaggg gtacggcaag tgctcgatgc gcatagcggc gctcccgttc
     2641 ttcttcatgt cgacgctacc cagctaccct ttgctgagtc ctttgaacgt acgcgtcttg
     2701 ccgctgacct tatatcgctt gatgcacaga aagtcggtgg cgtgcgggga attggctgcc
     2761 ttatcgtctc accacgcaca acgcttcttc cggctatcgg aggcggaggg caagagcgcg
     2821 gcctccgctc tggaacagaa actcctgcag cggctcttgc gtttgcgata gcgctcgaag
     2881 aagccgccgg tgcacgtgaa gcgtttaccg cccgagcgct tgctgcacgt aacgcatgca
     2941 ttgctaccat cacctcagct ctacctgacg ctgtggtgaa cgaaggaaaa gagaacgtag
     3001 cgcatatttt gaacttctca ttcccaggac gcgatactga tttcttagca gcacttcttg
     3061 atgaacgcgg cattgccgta tccacgagaa gcgcgtgcga aaccgactca gtggaagggt
     3121 cgcgtgcggt ccaggcactc accggcgact ctgcacgcgc cactgcaaca ctgcgcatct
     3181 cgtggggtcc tgttactaaa accgacgacc tcagacgcgc tatgaccgcc cttatcgaag
     3241 ccgttcgctt catcgaccac acccgtctct agtggtataa tgcacatata tggacataga
     3301 agacctttct aaaagtcagc ttctcctcct caccatactg gtaaacttcg tggtctctat
     3361 cgccacgggc gtgttaacgg tatcgcttct tgatgaggca ccgaccacgg tcacacaaac
     3421 ggtgaatcgc atcgttgatc acaccatcga gaccgtcacc acgcaggtcc caactattgg
     3481 aaaagattcg acaccgtcct ccgaagaatt gcttaccgct gccatcgccg aaaacgcaga
     3541 gcacatggtg accatttcgt accgtgtcgg cgctgaatcc actgtcgtaa acggactcta
     3601 tcttccaagt acgcgcctgg tgatgacagc atcaagcaat atgccaggac acataacggt
     3661 aacgttcccg aacggtgctg cggtagaagc agaccgcacc gcttcagacg gcacgctgat
     3721 gcgctacggt ttcgccgctg atgcagtgct cccgcaagcc tctactctca cactcgtccc
     3781 cgcgtcatcg ttgaagcaag ggcaaaccat tatcggccta tctgctgacg gcaaagcagt
     3841 caccggcatt atcggtaagg tggacacgga cggcattcat gcagaacttc ctgctgtctc
     3901 tcctggcgca cctatcgtga acctctcggg cgccgtaatc ggcatagccg ccggaggcaa
     3961 tcttttctat accgctgatc gcatcagtac attacaagcc gctccgtaag caattattca
     4021 ttcactcgtc ttcacctcta tgcccccatt caagaacttc actactaaag caaaagaagc
     4081 catccgcaaa gcgcatgagc tcgctatcga gcggggacag aaccatgtct caccgctcca
     4141 cctccttgct gccctcatcc accaggaaga gagcctcgtg ttttctgtac ttgatcgcat
     4201 ggatgtagac accatgctct tagctgacac gcttctagag aacctcgaag cacctgagac
     4261 cagctccacc ctttctccgt cgtatcagct ctatctcact cccgatcttg cgcagtctct
     4321 tgaggcgtca ggcaaagtcg ctgcacgcat gaatgatgcg tttgtgggga ctgagcacct
     4381 ctttgtagcg gtccttgagc atccgggacc tgcgggcgac attctttccc gcttcaagat
     4441 tgatgcagat accgtacagc tggttctgaa ggaattgaag aacgccaagg acggtcaggt
     4501 agtggaacca aagaagttcc gcgcactcgc gaaatacacg cgcaacctca cgaagcttgc
     4561 gtcagaaaat gcgcttgacc cggtcattgg acgcgatcag gaaattcacc gcgtgattca
     4621 gattctttct cgtcgcacca aaaacaatcc ggtcctcatc ggtgaagcag gtgtgggtaa
     4681 gacggctatt gccgaaggtc ttgcgtcgcg catggctaat aacgaagtgc ccgagtcgtt
     4741 gaagaacaag gaactcctct ccttagacct tggtctcatg attgcaggca caaagtatcg
     4801 cggtgagttc gaggaacgca tgaagaccgt gatgaaagaa gtagagcgtg cagaagggaa
     4861 ggtgattctc tttgtggacg agctccacac actcgtaggg gcgg
//
LOCUS       DAORIN010000044         4633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_3775_length_4633_cov_5.316951, whole genome shotgun sequence.
ACCESSION   DAORIN010000044 DAORIN010000000
VERSION     DAORIN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3775_length_4633_cov_5.316951"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PK109_02320"
     CDS             complement(<1..967)
                     /locus_tag="PK109_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24400.1"
                     /translation="MKRTPASKAHISIVGGAVLVLAGAFMVPHLAATTYDRGSDEKNF
                     VEKVAEHIKEEPKLDTEAYDKKMRFLAHVDETATTSTSTPKGLWPVTDAPYPKAGALL
                     PFNRIVAYYGNFYSTKMGVLGEYDKGTVLAKLKSEKAAWEAADPTTPVVMGIDYIAVT
                     AQGSPQADGTYKLRMPEEHIQRAIDMANEVDGIVILEIQVGLSTFQKEIPLLDKYLAL
                     PNVHLAIDPEFSMKGGEPPGDVIGTVSSADVNYAAQYLADLVREHDLPPKLLVIHRFT
                     QKMVTGAANIEPLPEVQMVMVMDGWGFGAKKINTYNSIIKPEPVQF"
     gene            complement(945..1142)
                     /locus_tag="PK109_02325"
     CDS             complement(945..1142)
                     /locus_tag="PK109_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24401.1"
                     /translation="MARSSILILLGILVLLTPYLGLPYAWLMVLLPILGLCVLLIGIT
                     LRGKRRAPVIAPESYETHSSV"
     gene            complement(1155..1835)
                     /gene="rsmI"
                     /locus_tag="PK109_02330"
     CDS             complement(1155..1835)
                     /gene="rsmI"
                     /locus_tag="PK109_02330"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRH24402.1"
                     /translation="MGTLSIVATPIGNLGDITLRALETLRQADVIACEDTRVSAKLLA
                     RYDIQKPLLIFHARSGKLASDRILSQLAEGKRIALMTDAGTPGISDPGNELVRVVRER
                     LGNDVRIESIPGPSAVAAALSIAGIHSNEFTFLGFLPHKKGRQTLFKEIADSERTVVF
                     YESPHRIEKALVSLREVLPEDRIVTVARELTKMHESLVQGNPAEVAAHYAAHPDEIRG
                     EFVVMVSS"
     gene            complement(1837..3105)
                     /locus_tag="PK109_02335"
     CDS             complement(1837..3105)
                     /locus_tag="PK109_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRH24403.1"
                     /translation="MSPKKAYFIGIGGIGMSALAQLLHHDGWNVSGSDREESPTTELL
                     KEKGMSVRIGQNAENVPADADMVIYTEAAWEDNAERMRAAELGLPQKSYFEMLGEVSK
                     GKRTIAIAGTHGKTTTTAMAARILSDAGVSPSAVIGSIVKDFKSNYLSGTSEWLVVEA
                     CEYKGDFLTLAPEVLVVTNIEWDHTDYYPNLEAVQESFLALMKKAAVIVTNPKDKNIA
                     PLLSGLSAKVIDYTQEPAYELALPGDFNQMNARAAVAAARIATPKITDKTIRTALSKF
                     EGTWRRFDYKGKTKRGALVYDDYAHHPTAIQKTLAEFKKKPHGTLYVAFHPHLFSRTR
                     DLLAGFAKAFKDADRVLIAPIYAAREEDDGTMSNSILARKIEENGTDAKAADFTEIRR
                     YFETEPGEGDIVVTMGAGDIYKVADELVAN"
     gene            complement(3110..3868)
                     /locus_tag="PK109_02340"
     CDS             complement(3110..3868)
                     /locus_tag="PK109_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRH24404.1"
                     /translation="MIGVFDSGSGGLTVLTEIRTRLPKADLIYFGDIRNAPYGPRSKE
                     ELLSLTGNALAFLQRHGATRYVSACNSVSTSLALSLLDTLSIGQGQIIEMVGPSVRSF
                     KGSTERIGLVATSATIDSGIYQNAFHMIGKEVEAIAIPELAGAIEAGARTEEVDRIVN
                     SALGEKTGVFDVLVLACTHYPLALESFKRELGSSTRLYNPAVAVGEKVEEAWGASEEG
                     TGTLRFFISQDSETFRGLVAQFFAEGTYTIEVIE"
     gene            complement(3865..>4633)
                     /locus_tag="PK109_02345"
     CDS             complement(3865..>4633)
                     /locus_tag="PK109_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRH24405.1"
                     /translation="EELSPHIAVFTNLFPDHQDYYEGMDDYLADKAEIFLHQGPDDTL
                     VLGSAVAELIKEKYVGKAIVSNVVIADPNAIGESELGIPGMHNRANAACALAAAEAFG
                     VSTDAAKKALASFTGVAGRLELIREKDGVRFYNDTTATTPEATLAALAALPETTGIVL
                     IMGGSDKGLDMNALLLAVGNRAKRIILLPGTGTNRVLEFLTGASVYDTLEAAVAEAAR
                     SAESGDAVLLSPAFASFGMFKNEYDRGDQFNALVRAL"
BASE COUNT         1037 a         1257 c         1178 g         1161 t
ORIGIN      
        1 tgaactgcac tggttccggt ttaatgatgg agttataggt gtttattttc tttgccccaa
       61 agccccatcc gtccatgacc atcaccatct gcacctcagg aagcggctca atgtttgcgg
      121 ctcccgtcac catcttctgc gtaaagcgat ggatgaccag tagcttcggc ggtagatcat
      181 gttcacgaac aagatcagca agatactgcg cggcatagtt cacgtcagca gaagacacgg
      241 taccgataac gtcgcccggt ggttctcctc ctttcatgga gaactcggga tcaattgcca
      301 agtgcacgtt cgggagcgcg aggtacttat caaggagtgg gatctctttc tggaacgtgg
      361 agagaccgac ctgtatttca agaatcacaa tgccgtccac ttcgtttgcc atgtcgatgg
      421 cacgctgaat atgctcttcc ggcatacgaa gcttatacgt gccgtccgcc tgcggacttc
      481 cctgcgccgt gacggcaatg tagtcgatac ccatcacgac gggtgtcgtg gggtctgctg
      541 cttcccatgc cgccttctca ctttttagtt ttgcgagcac ggttcctttg tcgtactcac
      601 caagcactcc catctttgtt gagtagaagt ttccgtagta cgcaacgatg cgattaaacg
      661 gaagcagtgc tcctgctttc gggtacggcg catcggtcac cggccacaac cctttcggtg
      721 tcgaagtaga ggtcgttgcc gtctcatcaa cgtgtgcaag aaagcgcatc ttcttgtcgt
      781 acgcttccgt atccagtttc ggttcttctt ttatgtgttc agcaactttc tcaacgaaat
      841 tcttctcatc acttccccgg tcatacgtgg tggccgcaag gtgtgggacc ataaacgcgc
      901 ccgcaaggac gagtaccgcc ccaccgacga tggagatgtg tgccttagac gctggagtgc
      961 gtttcatagg attcgggagc aatgaccgga gcacgacgct ttccgcgaag ggtaatacca
     1021 attaagagca cacacagacc aagtatagga agcagcacca tgagccaagc atacggaagc
     1081 ccgaggtacg gcgtaagaag cacaagaata ccaaggagga taagaatact gctgcgtgcc
     1141 atgcaggcag tctatcaaga cgacaccatc accacaaatt ctccccgtat ttcgtcagga
     1201 tgcgccgcgt aatgtgcagc gacttcagcg ggatttcctt gtaccaatga ctcatgcatc
     1261 ttcgtaagct cacgcgcaac ggtcacaatt ctgtcttcag gaagtacctc gcgcagtgag
     1321 acgagtgcct tctcgatacg gtgcggggac tcatagaaca ccaccgtccg ttcgctgtca
     1381 gctatctctt taaatagagt ctggcggcct tttttgtgag gcaagaaccc aaggaacgta
     1441 aattcgttac tatgaatgcc agcaatagag agtgctgcgg ctaccgcaga gggaccagga
     1501 atggactcga tgcgtacatc gtttccaagg cgctcccgta cgacgcgtac caattcattt
     1561 cccggatctg agatgccagg ggttccggcg tccgtcatga gcgcgatacg cttcccttcg
     1621 gcgagctggg aaaggatgcg atcagaggca agtttcccgc ttcgcgcatg aaagataagg
     1681 agtggcttct gtatgtcata gcgtgcaagg agttttgctg acactcgggt gtcttcgcat
     1741 gcgatgacat ccgcctgacg cagtgtctca agcgcacgaa gcgtgatgtc accgagattc
     1801 cctatagggg tagcaacgat agaaagagtt cccatattag ttggcgacga gttcgtccgc
     1861 taccttataa atgtcgcccg cgcccatcgt gacgacaatg tcaccctcac ctggttccgt
     1921 ctcaaagtac cgacgaattt cagtgaagtc tgccgccttg gcgtccgttc cgttctcttc
     1981 aatctttcgt gcaaggatgc tattagacat cgtgccatca tcctcttcac gcgctgcata
     2041 tataggagcg atgagtaccc gatccgcgtc tttgaatgct tttgcaaatc ccgccagaag
     2101 atcgcgcgta cgactaaaga ggtgcggatg gaatgcgacg tagagcgtgc cgtgcggttt
     2161 cttcttaaac tcggcaagcg tcttttgtat ggcagtcgga tgatgtgcgt agtcatcgta
     2221 gacgagagca ccacgcttcg tcttcccttt gtaatcaaat cgacgccagg ttccttcaaa
     2281 ctttgagagt gcggtacgta tagtcttatc cgttatctta ggcgttgcga tgcgcgctgc
     2341 cgctaccgct gcacgtgcat tcatctgatt gaaatctccg gggagtgcga gttcgtatgc
     2401 tggctcttgt gtgtaatcga taaccttcgc tgagagtccg ctaaggagtg gcgcaatatt
     2461 cttatccttc ggattagtaa ctatcactgc ggcctttttc ataagcgcga gaaacgactc
     2521 ttgcactgct tcaaggttcg gatagtaatc ggtgtgatcc cactcaatgt tcgtgaccac
     2581 cagcacttca ggagcgagcg taaggaagtc tcctttatat tcacaggcct ctactaccaa
     2641 ccattcagag gttccagaaa gatagtttga tttgaagtct ttgacgatgg aaccaatgac
     2701 cgctgatgga gagacgcccg catcacttaa aatgcgtgcc gccatcgcag tagttgtggt
     2761 cttcccatgc gtacctgcta tcgcaatggt gcgcttccct tttgatacct ctccgagcat
     2821 ttcaaagtat gacttctgcg gaagcccaag ctcagccgca cgcatgcgct ctgcgttatc
     2881 ctcccacgcc gcttccgtgt agatgaccat gtccgcatct gccggtacgt tctcggcgtt
     2941 ctgtccgatg cgtacgctca tgcctttttc tttaagaagc tcagtcgtcg gactttcttc
     3001 gcggtctgat cctgagacgt tccacccgtc atggtgtagc agctgcgcga gcgcgctcat
     3061 accgataccg ccgatgccta tgaaatacgc ctttttcggg gacatatcgc tactctatca
     3121 cttcaatggt atacgtgcct tctgcgaaga attgagcgac aagaccacgg aatgtttctg
     3181 agtcctggct tatgaagaag cgaagtgtgc ccgttccttc ttctgatgct ccccaggcct
     3241 cttctacttt ctctccgaca gcaacggcag gattataaag gcgcgtgcta ctcccaagtt
     3301 cacgtttaaa actttcgagc gcgagcggat agtgcgtgca cgcaagcact agtacatcaa
     3361 atactcccgt cttttctcca agcgcgctgt tcacgatacg atccacctct tctgtacgcg
     3421 ctcctgcttc tatagccccg gctaactcag gaatagcaat tgcctccact tccttcccaa
     3481 tcatatggaa cgcgttctga tagataccgg agtctatggt cgcactcgtg gcgactagac
     3541 caatgcgctc ggtacttcct ttaaaggaac gaacgctcgg gcccaccatc tctattattt
     3601 gtccctgccc tatagaaagc gtgtcgagga gcgaaagcgc tagcgatgtg gacacactgt
     3661 tacaggcgct cacatagcgg gtcgcgccgt gccgctgcag aaacgcgagt gcgtttcccg
     3721 taagtgagag gagttcttct tttgagcggg ggccgtacgg ggcgtttcgt atatcgccaa
     3781 aatagatgag gtctgcctta ggcaagcggg tacgtatttc tgtgagaacc gtaagtccac
     3841 ccgagcctga atcgaaaaca ccgatcatag ggcgcgcacc agcgcattaa actggtcgcc
     3901 tcgatcatac tcattcttga acataccaaa ggaagcaaac gcaggagaaa ggagtacggc
     3961 gtctcccgac tctgcggaac gcgccgcttc tgcgactgcc gcctcaagcg tgtcatatac
     4021 cgacgctcct gtaaggaact cgagcacgcg attagtgccg gtgccgggaa gaagaatgat
     4081 tcgttttgct cggttcccta ctgcaagcag caatgcattc atgtcgagcc ctttatcgga
     4141 tccacccata ataaggacga tgcccgtagt ttctggaagc gctgcaagtg cggcaagggt
     4201 tgcctcaggc gtcgttgctg tcgtgtcgtt ataaaacctc acgccatctt tttcacgaat
     4261 gagttcaagg cgacccgcca cacccgtaaa ggaggcgagt gcctttttgg cagcgtcagt
     4321 tgataccccg aacgcttcag cggcagcgag tgcgcacgca gcgtttgcac gattatgcat
     4381 tcccggaata ccaagttcac tttctcctat ggcgttcgga tcagcgatga caacgtttga
     4441 gactatcgct ttgcctacat acttttcttt aatgagttcg gcgacagcgc ttccaagtac
     4501 gagtgtgtcg tcggggcctt ggtggagaaa tatctctgcc ttatcagcca ggtagtcatc
     4561 cataccttcg tagtagtcct ggtgatcggg gaaaagattc gtgaacactg caatgtgtgg
     4621 tgaaagctct tcg
//
LOCUS       DAORIN010000045         4453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4028_length_4453_cov_4.829923, whole genome shotgun sequence.
ACCESSION   DAORIN010000045 DAORIN010000000
VERSION     DAORIN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_4453_cov_4.829923"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PK109_02350"
     CDS             <1..208
                     /locus_tag="PK109_02350"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010883618.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRH24406.1"
                     /translation="GIFGMNAAGFKQYIEHIADRRLERVGLPAQYHVENPFQWMSEAV
                     DLNKEKNFFETRVTEYKTGGQLSW"
     gene            288..545
                     /locus_tag="PK109_02355"
     CDS             288..545
                     /locus_tag="PK109_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24407.1"
                     /translation="MNWLLAVPGVAMLVASFVFHRLAKLEEVKLKKLEGKGTLGNATI
                     VEKKRRLHHTVVQLHSWSVGFLFGGILAVAIGPYFFECVDF"
     gene            complement(726..2771)
                     /locus_tag="PK109_02360"
     CDS             complement(726..2771)
                     /locus_tag="PK109_02360"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRH24408.1"
                     /translation="MTLFGVRLFYPLSGRQWRAGILLLVAYVAATRLSAAAFAAPGLI
                     FPASGFALGGLFLEGFALWPFVFVASLIGYVLSGASPVYLLFLPIAHTLQAVLGAYLL
                     RTFKLDPLFRRSKDMFTLIGVALVASVVVPTIGTVARHLHYHLYNLPPSTVTWASWYA
                     ATVFSLLIISPFVIRWFAKPRFSRTFWQWIEVLVPFGFLATLSYFLFFTSSTSVVGIS
                     LAYFILIPLFWIALRLRPRFITLALLILSCFALSSLYVGPLAVEHAVLGMRLFQTEIF
                     LNIVAIIFFILSSIEEERRMTTNLMKTQMESLSTALAKVAYQDKAKSEFMAMLAHELR
                     NPLAPVVSSVDLLRLSEPAGSERAGILEVMDERLRAVRRLLDDLLDVTRINENKLSLQ
                     KEKIAIQNVVERAILSTKGHFAERTQTLSVNLPEDRLIVHVDPLRIEQAVTNLLTNAS
                     KFSEEGSAITLEVAQDHQWVTVRVTDTGVGIPSSMLASIFDPFLQIEQGERTRKGIGI
                     GLSLVKSFVEMHDGSVEAVSGSQGSGSTFSIRLPLLRVERASGAPSPSVAATPASMTH
                     CSVLVVDDNDAAAWGVGKLLELSGCTIEYAYDGEQAIQKALNTGPDAVLLDIDLPDHD
                     GYHVAKELRAKGYTGAIIALSGYSLEQDRQRGRASGFTDYLVKPVGLADLRRVLPLK"
     gene            2991..3611
                     /locus_tag="PK109_02365"
     CDS             2991..3611
                     /locus_tag="PK109_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24409.1"
                     /translation="MSILSIALMGFFAFSGTPSAPVSAPTTELSADVATTSALALKDS
                     AVVIRPSDTTEPLSVLMTSYNAVPEQTDGNPMVTASGAVSNPEVIAARSVDLAGELPF
                     GTVIAVEYDGKDTENCRFHAVEGLIGYRVIGDSMNSRMRNKIDILLDRADTVTVHGKE
                     TNPSLALGLCKGVTVRVVGRIKVSDIPDTQEELRKMIEGDSLAINR"
     gene            3767..3970
                     /locus_tag="PK109_02370"
     CDS             3767..3970
                     /locus_tag="PK109_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24410.1"
                     /translation="MRGKKQRKRKLREAKLEPARALRRKERIKKRRELRAAARKGTKK
                     VSKSKKPNAATRQSTQPPVSLAA"
     gene            4061..>4453
                     /locus_tag="PK109_02375"
     CDS             4061..>4453
                     /locus_tag="PK109_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24411.1"
                     /translation="MAHRISVDSRADHSRAGRIFATLKERYPKTKLTGVTLTRAYTID
                     AKLSPRDLTRAVAVLTNPVIETSTEKRVLSPNKFAYAIDIGFRPAVTDNAGTTARQMV
                     EDAIGKKLRDGENVYSSYFVFLQGTVTKA"
BASE COUNT         1111 a         1148 c         1230 g          964 t
ORIGIN      
        1 aggcatcttc ggtatgaacg ctgcgggctt caagcagtac atcgagcaca tcgctgatcg
       61 ccgtcttgaa cgtgtgggac ttcctgcgca gtaccacgtt gagaacccat tccagtggat
      121 gagtgaagca gttgacctta acaaggagaa gaacttcttt gagacccgcg tcaccgaata
      181 caagactgga ggtcagttga gctggtaggt gcaaaattcc atagttgtgg cataatacgc
      241 cacaggatgt tcttgcacga gagaacttcc agaccgggag ctagaaaatg aattggttac
      301 tcgccgtacc aggcgtagcg atgctcgtag cgagcttcgt gttccacagg ctggcaaagc
      361 ttgaggaagt aaaactcaag aagttagaag gcaaaggcac gctcgggaat gccaccattg
      421 ttgagaaaaa aagaaggctc caccacacgg tggtacagct ccatagctgg agcgtaggtt
      481 ttcttttcgg cggcattctc gccgtcgcca ttggtcccta ttttttcgag tgcgtcgact
      541 tctaatcggt caaaacagcg ctcgcttggc ttcggccttg cgagcgtttg ttcatatata
      601 taacgagaaa ctccaccgca gcggagtaat ctctgtagga catggtactg gcttagacgg
      661 ctaaagctgg tgtcggctca agaaccccat cctatcgagg caggcatgaa ccctagatga
      721 agggtttatt ttagtggcaa tacacgtcgt aaatcggcga gccccaccgg ctttacgagg
      781 tagtcggtaa acccagacgc acgccctcgc tggcgatcct gctcaaggct atacccggac
      841 agcgcgatga tggcacccgt gtatcccttt gctcgaagct ccttagcgac gtgatagccg
      901 tcgtggtcgg ggagatcgat atcaagcaat accgcgtcgg gtcccgtatt cagggctttt
      961 tgtattgctt gctcgccgtc ataagcatac tcaatcgtgc atccagacag ctcgagaagt
     1021 ttcccgacac cccacgcagc agcgtcatta tcgtcaacca ccaggacgga gcagtgggtc
     1081 atgcttgcag gtgttgcggc aacgcttgga gaaggtgcac cagaggctcg ctcaaccctg
     1141 agtaggggaa gtcgtattga gaaggtgctg ccgcttcctt gacttccact cacggcctcg
     1201 acgctcccgt cgtgcatctc tacgaaactt tttacaagcg acagtccgat gccgatgcct
     1261 ttacgcgtgc gctcaccctg ttctatctgc aagaacggat caaaaatact tgcaagcata
     1321 gaagaaggta taccgacgcc ggtatcagtc acccggaccg ttacccactg atgatcttgc
     1381 gcgacctcta gcgtaatagc gcttccttcc tcagagaact tagaagcgtt cgtaagtagg
     1441 ttggttactg cctgctcaat gcgcagcgga tccacatgca cgatgaggcg gtcttcagga
     1501 aggttcactg agagggtctg ggtacgttcg gcaaagtgtc ctttggtaga gaggatagcg
     1561 cgctccacca cgttctgtat cgctatcttt tctttctgga gtgagagctt gttttcgttt
     1621 atacgcgtta catcgagaag gtcatcaagg agacgtcgca ctgcgcgtaa gcgctcatcc
     1681 attacttcaa ggatgcccgc ccgctcactt cccgcaggct cagaaaggcg tagcaggtca
     1741 acagaactca ctacgggcgc cagcgggttg cgcagttcgt gcgcaagcat cgccatgaac
     1801 tcactctttg ctttgtcttg gtaggcaact tttgcaagcg ctgttgaaag cgattccatc
     1861 tgtgttttca tgaggttcgt ggtcatgcgc cgctcttctt ctatagaaga aaggatgaag
     1921 aaaatgatcg ccacgatgtt gaggaatatt tctgtctgaa ataggcgcat accaaggact
     1981 gcgtgttcaa ctgctaacgg accgacatag agactgctga gggcaaagca ggacagtata
     2041 aggagagcaa gtgtgatgaa acgcgggcgc aaccgcagcg ctatccagaa gagggggata
     2101 aggatgaagt aggcaagcga gataccgacg acacttgttg aggaagtgaa gaagaggaag
     2161 taggacaggg tggcaaggaa cccgaacgga accaggacct ctatccactg ccagaacgtg
     2221 cgggagaatc gaggcttcgc gaaccaacga ataacaaaag gggagatgat aaggaggctg
     2281 aataccgtgg cggcatacca ggacgcccag gttacggttg agggcgggag gttatagagg
     2341 tggtagtgga ggtggcgtgc taccgttccg atggttggaa ccaccactga tgcgacgaga
     2401 gcgactccga taagcgtgaa catgtcctta gaccgccgga acaaaggatc gagcttaaag
     2461 gttctgagaa ggtacgcgcc gagaactgcc tgaagcgtgt gggctatagg gaggaagagg
     2521 aggtataccg gagaggctcc cgagagcaca taccctataa gggaagcgac aaagacaaat
     2581 ggccagaggg caaatccttc aaggaagagt ccgccgagcg caaagcctga ggcagggaag
     2641 atgagtccag gggcagcaaa agcagccgca gagagccgtg tggccgccac ataggccact
     2701 aacaggagga taccggcccg ccactggcgc cctgagaggg gatagaagag ccgtacgcca
     2761 aagagggtca tataagggga ggaggggtaa ttgcctcatt actatagcag ctatggtgaa
     2821 atgggcatga aaataggccg tcttataggg tacctccagg aaatggctta taataagggt
     2881 ttctggacga ataaactatt gacataataa atatatatgt cgtgggtatt gacaaaatga
     2941 acccccgatg atatcctagc ccttgtaagg cggttcgcca ctttacgttt atgtctatcc
     3001 tttctatagc ccttatgggc ttttttgctt tttcggggac cccttcggcc cccgtttcag
     3061 cacctaccac cgaactgtcg gcggacgtcg ctaccaccag cgcgctcgct ctcaaggatt
     3121 cggcggttgt tatccgtccc tcggatacga ccgaacccct ttccgtactc atgacgagct
     3181 ataacgccgt cccggaacag accgacggta accctatggt gaccgcttcc ggtgctgttt
     3241 ctaaccctga ggtcatcgcc gcccgttccg ttgaccttgc tggggagctt ccgttcggta
     3301 ccgtcattgc cgttgaatac gatggcaagg atactgagaa ctgccgcttc catgcggtgg
     3361 agggtctcat cgggtaccgt gttatcggtg actccatgaa ctcccgtatg cgtaacaaga
     3421 tcgacatcct tcttgaccgt gccgacaccg ttacggtcca tggtaaggaa acgaacccct
     3481 ccttggcgct cggcctttgt aagggagtta cggttcgtgt cgtgggccgt atcaaggtct
     3541 ccgatatccc tgacacccag gaggagctcc gtaagatgat tgagggcgac tcacttgcga
     3601 taaaccgcta atacaaggct tctgaaacac cgcacccgaa agggtgcggt gtttcttttt
     3661 acccacgccc acattgactt atcttaagtc ctcgctagta tcggacgtta tggaggtttc
     3721 ggcctcctgt tttcactaag taccaacgaa ataggggaaa acgcacatgc gtggaaagaa
     3781 gcaacggaag cgtaagcttc gcgaagctaa attggaaccc gcacgagcac tgcggcgcaa
     3841 ggaaagaatc aaaaagcgcc gtgagctgcg ggctgccgca cgcaaaggca ccaagaaggt
     3901 gtcgaaaagc aagaaaccaa acgcggcaac gcgtcaatcc acgcaaccgc ccgtttcgct
     3961 cgctgcctaa gggtacgagc atacggaata caggggcgcc gcgagctatc gcggcgccct
     4021 cacttgttac gtgctaaaat cctggcaacc cactcctggc atggcgcacc gcatttccgt
     4081 cgattctcgt gcagatcatt cccgcgccgg acgcattttc gcgaccctca aagaacgata
     4141 cccaaagacg aaactcaccg gagttactct tacgcgtgcg tacaccattg acgcaaagct
     4201 ttcaccacgt gatcttacgc gcgctgtcgc ggtgctcacg aacccggtta ttgaaacatc
     4261 gacggagaag cgagtgcttt caccaaataa atttgcatac gctatcgaca taggattccg
     4321 tccggcagtg acggataatg cgggcaccac cgcgcgtcag atggtagagg atgccatcgg
     4381 gaaaaaactt cgtgatgggg agaatgtgta ctcctcctat tttgtatttc ttcaaggaac
     4441 ggtcacgaaa gct
//
LOCUS       DAORIN010000046         4410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4098_length_4410_cov_7.355683, whole genome shotgun sequence.
ACCESSION   DAORIN010000046 DAORIN010000000
VERSION     DAORIN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4098_length_4410_cov_7.355683"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PK109_02380"
     CDS             complement(<1..623)
                     /locus_tag="PK109_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRH24412.1"
                     /translation="MDAGAYFKDKRITVMGLGLLGRGVGDVRYLARSGADIIVTDLKS
                     KEELAPSLAVLAEFPNIRYTLGEHTFEDFQDRDLILKAPKTPLDSPYIEEAEKNGIPV
                     TMSTALFARLAREAGVALIGITGTRGKTTTTEMIAHILREQGNDVLLGGNVRGVSTLA
                     LLPDAAPGSVAVFELDSWQLQGFRAEELSPHIAVFTNLFPDHQDYYEG"
     gene            complement(660..1145)
                     /locus_tag="PK109_02385"
     CDS             complement(660..1145)
                     /locus_tag="PK109_02385"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HRH24413.1"
                     /translation="MRDVLIIGIACAAAILLGAWLYFSDPHVTGQSDVPAFTILDEGM
                     YSGEVTERKNYRIKSQEELEALWLLVHGTGAPTNVDFARSEVIAVFDGTHATGGYGIS
                     IASVADVAGSPRVITILHTEPGTSCLTTDAISSPYQIAIVSKSAAPLTREEETIVTEC
                     N"
     gene            complement(1201..1276)
                     /locus_tag="PK109_02390"
     tRNA            complement(1201..1276)
                     /locus_tag="PK109_02390"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1241..1243),aa:Ile,seq:gat)
     gene            complement(2133..3590)
                     /locus_tag="PK109_02395"
     rRNA            complement(2133..3590)
                     /locus_tag="PK109_02395"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            3770..4117
                     /locus_tag="PK109_02400"
     CDS             3770..4117
                     /locus_tag="PK109_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24414.1"
                     /translation="MVLLLIDVLEELEVSVFNHTFGTLYLEKQGAETEIKIGDHIWIT
                     QIHAGKSQDAIRIERDKKSLWFFHNDEWRDSWSVGDIDAPDLFLFDLLHELGLDMVPY
                     DDGWTVIKQAEQM"
     gene            4194..>4410
                     /locus_tag="PK109_02405"
     CDS             4194..>4410
                     /locus_tag="PK109_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRH24415.1"
                     /translation="MSKKVVAVSGGFDPIHIGHIRYIKESRALGDHLVVILNNDNWLQ
                     NKKGFVFMPEQERKEILEAIDGVDEVII"
BASE COUNT          998 a         1191 c         1123 g         1098 t
ORIGIN      
        1 ccttcgtagt agtcctggtg gtcggggaaa agattcgtga acaccgcaat gtgcggcgag
       61 agctcttcgg cacggaatcc ctgaagttgc caggaatcga gctcaaagac cgcaacactt
      121 ccgggagcag cgtcaggaag aagcgcgagc gtggaaaccc cgcgcacgtt tccgccaaga
      181 agcacatcgt tcccttgttc gcgcaggata tgcgcaatca tttctgtcgt agtggtcttc
      241 ccgcgtgtac cggtaatacc aatgagcgca acgcccgctt cgcgcgcaag tcgtgcaaac
      301 aaggccgtcg acatcgttac cggaataccg ttcttttctg cttcttctat atacggagaa
      361 tcgagcggcg tcttgggcgc ctttagtatc aagtcgcggt cctgaaagtc ttcgaacgta
      421 tgttcaccga gcgtgtagcg gatattcggg aactcagcga gtacggcaag cgacggcgcg
      481 agctcttctt ttgacttaag atcggtgacg atgatgtcgg cacctgagcg cgcaaggtag
      541 cgcacgtccc cgactccgcg acccaatagg ccgagtccca tgacggtaat acgcttatct
      601 ttgaaatacg ctcctgcatc catagcaagc atcgtagcgc acccctttaa aaaggcgaac
      661 tagttgcact cagttacgat agtctcttct tcgcgggtaa ggggtgctgc tgattttgaa
      721 acgatggcta tctgatacgg acttgagatg gcgtctgtcg tgaggcaaga agtacctggt
      781 tcagtgtgga gaatagtgat gacgcgtgga cttcccgcaa catctgcaac ggacgctatg
      841 gaaatgccgt agccaccggt agcgtgtgta ccatcaaaga ccgcaatgac ttcgcttcga
      901 gcaaagtcga cattcgtcgg cgctccggtg ccgtgcacca ggagccaaag cgcttcaagt
      961 tcttcctgac tcttgatgcg atagttttta cgttcggtta cttcgcctga atacatgcct
     1021 tcatcaagga tagtgaaggc aggaacgtct gactggccgg taacgtgcgg atcagagaag
     1081 tagagccatg cgccaagcaa gatggcggcg gcacacgcaa taccaatgat aaggacgtct
     1141 ctcataaaag cagtatagcg ccctttatag gcgctatccc gctcgggtgt acataatgct
     1201 tggtgcacgc tctaggattc gaacctagga ccgctcgagt atcagtcgag tgctctacca
     1261 actgagctaa gcgtgcgtat tggcgatact agcagaaatc cccagctact tccagcttcc
     1321 ctatttaggg ctgctatagt ttttaaagga atgattcacc gcgctagagc gagagaatcg
     1381 aagaggctcc ggcccatgac ccggacttct actcttgagg aaaagctgag acgacataag
     1441 aataagcgac gtatttggcg gcggtgcgtg gcgcgtctcg cgctcgctca agcatctata
     1501 taaaagaaga cggggacgcg catatgctgc gcggccccat cgaaagtctt gtcatttgag
     1561 ccgcgcttgc gcggccgtcc tgcccgcttc agtcagttgt acgtagccgc cggaagtttc
     1621 ttcgaggaca tatcttttct tcagaagcca atcgaaatgc tcttgcctat cgtcgatgag
     1681 ccgtggtccc gtatagggtt cattgttctc tcgacgccgc tcgctttcat agagagcggt
     1741 cagaaggcct aacgatcggt ttttcaacga cggttccagg tacatgctga tgcgaggcca
     1801 acctcgaatg aacctagtgt gaagcttgag gcccatgatg ctttctccaa gccgcaacgt
     1861 gcgactttac cgggagacca tagcactctg caaagaaaag caaaagcccc tttcggggct
     1921 tttgcttttt gtaccaaaat gaaagtaagg aggaatagcg cgctcttgtg ttccgcctat
     1981 tcccatcctg cgagtcgtca attgagcgac tcgagaatgt cccgggatac gttatccggt
     2041 gtccgagtag tatttaagga taactactat gtcctctagt gtcttgcgac acagatcgac
     2101 cggatgagcg cctttcgggc taactcgttc cgattggata tgttccacga gcagcttccg
     2161 ctacccgtgc cttgttacga cttactccct gtcattgaat ttgccgtagt cctgcaaaaa
     2221 gcaagatatc gggcacctcc aacttccttg agttgacggg cggtgagtac aagactcgag
     2281 aacgtattca ccgtagtatg gctgacctac gattactagg gattccggct tcaagaggtc
     2341 gttgcagacc tctatccgta ctggggtcgg tttttgggat ttgctccacc ttgcggtctt
     2401 gcgtccttct gtaccgacca ttgtatcacg tgtgcagccc aaggcatcaa gggacatgct
     2461 gatctggcgt catcctcacc ttcctccccc gtgagggggc agtctcgcct gacataagta
     2521 acagacaacg agggttgcgt tcgttacccc actgaaggga acagctcaag ccacgaactg
     2581 acgacaacca tgcatcacct gccaagcacc ctcgaaggct ccgtcccttt caggtcggat
     2641 gcagcttggt ttctagccct ggtaaggttc ttcgcttagc tacgaattaa gccacatgat
     2701 ccaccgcttg tgcgagtccc cgtctattcc tttgagtttt aatcttgcga tcgtactacc
     2761 caggcggctc gcttaccgcg ttagcttagt ctcacagagg gtcgatactc cgtaaaacga
     2821 gcgagcaacg tttagggcca ggactactgg ggtatctaat cccattcgct accctggctt
     2881 tcgtgtgtga gcgtcagatg agggccagtg cgctgccttc gcctttggtg ttccccacga
     2941 tatcaacgga tttcacccct acaccgtgag ttccacgcac ctctccctat ctcaagtcat
     3001 gccgtttcgc ttgcaggccg gaggttgagc ctccggattt aacaaacgac taacatgacc
     3061 gccttgacac cctttacgcc caatgattcc ggataatgct cggggcctct gtattaccgc
     3121 tcctgctggc acagagttag gagcccctta ttcatgtggt accgtcaata atttcgtccc
     3181 acataaaagg tgtttacgtg ccgaagcact tcatccacca cgcgacgtcg ctccatcagg
     3241 ctttcgccca ttgtggaagt ttcttgactg cagcctcccg taggagtatg ggccgtgtct
     3301 cagtcccatc ggtgggggtc aacctctcag ttcccctaaa cgtcgtagcc ttggtgagcc
     3361 attacctcac caactagctg atattgcgca cgccgctccc gaagcgataa atctttaacc
     3421 ttccggtacc atccggtatt acctcacctt tcggtgagct attccagact tcggggtgcg
     3481 ttcgtacgtg ttactacctc gtctgccggt tgccttgcgg cccccttgac ttgcatgcct
     3541 tatccacgtc gccagcattc atcctgagct aggatcaaac tctcattaag aataggcgga
     3601 acacaagaat tcacttgttt cctccttacc tttctatttt ggtctctctt atttagtttt
     3661 caagggacga gcgaactgcc cgacccacgt aagtgggccg ataggaagat actacccttc
     3721 cctattgggg gcgtcaagcg acgttttgac ggcctaggta agccgtgtta tggtattgct
     3781 actgattgat gtcttggagg aattggaagt gtcagttttc aatcacacat tcggaacgct
     3841 ctatcttgaa aaacaaggag cggagactga aatcaagatc ggtgaccaca tatggattac
     3901 ccaaatccat gccggtaagt cacaagatgc gatacgcatt gagcgcgaca aaaaatcact
     3961 ctggttcttt cacaacgatg agtggcgtga ctcatggtcg gtgggcgaca ttgatgctcc
     4021 cgatctgttc ttgttcgacc tactgcacga actcggtctc gacatggtgc cctacgacga
     4081 cggatggacc gtgattaaac aagctgagca gatgtagtca ccgcatctta ctgcgcgcgc
     4141 agcctcggct gtgcgcgttt tcttatacga gccattccgt ctatacttcc cctatgagta
     4201 agaaagtggt cgccgtctcg ggcggattcg atcccatcca cattgggcac atccgttaca
     4261 tcaaggagag tcgcgcactc ggcgatcatc tcgtcgttat attgaataat gataattggc
     4321 tccagaacaa aaaagggttt gtgttcatgc cggagcagga gcgtaaagag attcttgaag
     4381 cgattgatgg tgttgacgaa gtcatcatca
//
LOCUS       DAORIN010000047         4363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4170_length_4363_cov_5.672470, whole genome shotgun sequence.
ACCESSION   DAORIN010000047 DAORIN010000000
VERSION     DAORIN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4170_length_4363_cov_5.672470"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..757
                     /locus_tag="PK109_02410"
     CDS             71..757
                     /locus_tag="PK109_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRH24416.1"
                     /translation="MQAVIMAAGEGKRMRPLTLERPKPLIEVSGKPIIEHVIDALPAE
                     IDELIIVIGYKGDMIKKHLGDSYGGKKIRYVHQWMPAGTAHALSLARPFLSGKFLLLN
                     ADDILGSEAIQEAMTHPLSIMAIRHDQPEKFGVIKTRADGTMESIVEKPTEFIGNLVS
                     TGAMVLDDRLFGYEAAKHEATGEYFMTHPLSLMAAEHPVVVVEQPLYIPVGYPEDIPK
                     AEAKLKELGR"
     gene            792..865
                     /locus_tag="PK109_02415"
     tRNA            792..865
                     /locus_tag="PK109_02415"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:826..828,aa:Met,seq:cat)
     gene            1000..1073
                     /locus_tag="PK109_02420"
     tRNA            1000..1073
                     /locus_tag="PK109_02420"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1034..1036,aa:Asp,seq:gtc)
     gene            1149..1904
                     /locus_tag="PK109_02425"
     CDS             1149..1904
                     /locus_tag="PK109_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24417.1"
                     /translation="MSAETPEGLKRPEVPVEMKPYLIEGSDFVFDSKKIVDALESGTL
                     DNETFAKSIGLTTVAQRLAAWEATTEEQWEKTKKLLGERMFARHLKRKDTMLAQVTAR
                     PGYTPVLDNTQVSIQSSNPEALSKDIATWLGGHSALVVDSDTKKSLNPDEVQIALNKT
                     DHFVDGSYGSAHVELTLEYPALNHALKLTYPSKDQEQVSVGMTFYDHAYGKDKISCQT
                     DILSVLKLLNYLKEKGHSTSCSLSQDLRPFVVQ"
     gene            2154..2768
                     /locus_tag="PK109_02430"
     CDS             2154..2768
                     /locus_tag="PK109_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24418.1"
                     /translation="MPPEAAPILPSEEERERIRHAIEKAKVECVYQELEKGKVLPSKV
                     NVAKFGSFPIPTINILQLMATLCADYLANDAFADKYENHDERRAWRDELTTREGFGPV
                     VAEFFKKGNRLSSNKIQGLQSGARVVQARLTTGVLADDHAKALAREVNECLEEFHSAF
                     DSGLIAHYNAYNPETKKAHLSPKEKHEITKKISALAERYLSLVT"
     gene            complement(2778..3167)
                     /locus_tag="PK109_02435"
     CDS             complement(2778..3167)
                     /locus_tag="PK109_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HRH24419.1"
                     /translation="MNELFRKVAHYTSTLMGTPSAFIVSCGIVVLWAVTGPYFNYSDT
                     WQLIINTGTTVLTFLMVFLIQNTQNRDAHAIHLKLDEIIRSIDLARNELIDIEEETDA
                     NIGKYEDEFRTIRSRKVARTEEEKDAA"
     gene            complement(3175..3378)
                     /locus_tag="PK109_02440"
     CDS             complement(3175..3378)
                     /locus_tag="PK109_02440"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRH24420.1"
                     /translation="MNASGTCSCHHHKLVPLMIVLIGLAFLLRALDIVDGDIIAIVWP
                     VLLIVAGLSKLAGHKCACCGKMQ"
     gene            complement(3452..>4363)
                     /locus_tag="PK109_02445"
     CDS             complement(3452..>4363)
                     /locus_tag="PK109_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRH24421.1"
                     /translation="GKVGELLIEEFAGIPVEVELGSELRYRRNTMHPEETLLLAVSQS
                     GETLDTIEAIREAKRHGVMTLGIVNVVGSSIARETDAGVYNHAGPEISVASTKAVISQ
                     MTVLALIALTLGRQKGLSTTDGQSFVAELNRLPALAREILSRRDHIQAIAKKYVAVEN
                     AFYLGRKYQCPVAYEGAIKLKELAYVHAEGNASGEMKHGPIALIEPSFLTVVLVPGDS
                     MYEKSKSNIQEIRARGGKVIAITTDGHEDDLTGLADDVISVPPTHEALLPILTAMPVQ
                     LFAYYVALERGLPIDQPRNLAKSVTVE"
BASE COUNT         1155 a         1077 c         1137 g          994 t
ORIGIN      
        1 tcatcgttca tttggaagga aggagcagta gattccatat ctgtcgatta tatcgtatag
       61 tcttcgcatt atgcaggcag taatcatggc cgcaggcgaa gggaagcgta tgcgtccgct
      121 cacccttgag cgtcccaagc cgcttatcga agtatcgggg aaacccatca tcgaacatgt
      181 catcgacgcg ctccctgctg aaatagatga gctcatcatc gtcatcggct acaagggtga
      241 catgatcaag aagcatctcg gggactcata tggtggtaag aagatccgtt acgtgcacca
      301 atggatgccg gcaggaacgg cgcatgcgct atctcttgcg cgcccgtttc tttctgggaa
      361 gtttctcctc ttgaatgccg acgacatact agggtccgaa gcgatacagg aagctatgac
      421 gcatccgctc tccatcatgg caatacgtca tgatcagcca gagaagttcg gggtcattaa
      481 gactcgagcc gacgggacga tggagtccat tgtcgagaaa ccaacagagt tcatagggaa
      541 tctggtgtct accggagcta tggtgctcga cgacagactc ttcggatatg aagcggctaa
      601 gcacgaagcg acgggcgagt acttcatgac ccaccctttg agcctcatgg cagcagagca
      661 cccggtggta gtcgttgagc agccgctcta tatccccgta ggatacccgg aagacatccc
      721 taaggctgaa gcgaagttaa aggagttggg gcgttgaagg gaagggggac gtagtataag
      781 gtaaagcaat tgctggtgta gctcaggtag ttagagcatg ggactcataa acccaaggtc
      841 gaaggtgcaa gtccttccac cagcacacgt aagcgccccc tcgggggcgc tttgcttatc
      901 ggcaggcctt gcagtacccc ctaaaaaacg ctatattcct ccaagttcga cccgcgggtg
      961 acacgcgctt atgccgtctc tctcgcgtac gaatgaattg cgggagtagc tcaactggtt
     1021 agagcgccgc cctgtcacgg cggaggttgc gggttcaagt cccgtctctc gcgcagatga
     1081 aagaaataca gaaagaccgc ccaacgaggc ggtctttctg gttcaaacga aagaggtata
     1141 cttatttcat gtcagctgaa acccctgaag gactaaaaag gccggaggtt cctgtagaaa
     1201 tgaagccgta ccttattgag gggtcggatt ttgtttttga ctcaaagaaa atagttgatg
     1261 cattagagtc aggaacttta gataatgaga catttgcaaa atctattggc cttactacag
     1321 tcgctcaacg gcttgcagca tgggaagcca ctacagaaga gcagtgggaa aaaacaaaaa
     1381 agcttctagg ggaacgtatg tttgccaggc acttgaaacg taaggacacg atgcttgcgc
     1441 aggtgacagc gcgtcccgga tatactccag tgctagacaa tacccaggtc agcatacagt
     1501 cgtctaaccc agaggctctt tctaaagata tagcgacctg gttaggaggg cacagtgcat
     1561 tagttgtcga ctcggacacc aagaaaagtc tcaatccaga tgaagttcaa attgcattaa
     1621 acaagaccga ccactttgtt gatggctctt acggatcagc tcatgttgag cttactcttg
     1681 aatatccggc attgaaccac gcactaaaat tgacctatcc ttctaaagac caagaacagg
     1741 tatcagtagg gatgactttt tatgaccatg cttatggtaa ggacaagatt tcttgccaga
     1801 cagacattct ttctgtgctg aagttgctca actacttgaa ggagaaagga cactctacct
     1861 catgctctct tagtcaggat cttcgcccgt tcgttgttca ataggtaggg aataaagata
     1921 cggccttggt acgccctaat cctggttcca acatcgcata ctagagcgcg cactacagga
     1981 ctaacgtcct agaatacaga aagaccgccc aacaaggcgg tctttctggt gtaagcgtct
     2041 gtccccatag ccatattcca gaagccagta gaaccgagcg agggtggacg cagcaggcac
     2101 tacctaccta gacagatgaa cgagaggaac gtagtctttt cggtactacc cctatgcctc
     2161 cagaagcagc gcccatactg ccaagtgaag aagaaagaga acggattaga cacgcgatag
     2221 agaaagcgaa agtagaatgc gtatatcaag agctcgagaa agggaaagtg ttgccctcaa
     2281 aggtgaatgt tgcaaagttc gggtccttcc cgattccgac gatcaacata ctccagctca
     2341 tggctacctt gtgtgccgat tatctagcaa atgatgcatt tgctgacaaa tacgagaatc
     2401 atgatgaacg ccgtgcgtgg cgcgacgagc ttaccacccg agaagggttc ggacctgtcg
     2461 ttgcggagtt ctttaagaag gggaatcggt tatcgtccaa taaaatccaa ggcctacaaa
     2521 gtggggcgcg agtagtacaa gcacgactca ctactggtgt cctcgcagac gatcacgcta
     2581 aagcgctcgc aagagaagtg aatgagtgcc tagaagagtt tcatagcgcc tttgatagcg
     2641 gtctcattgc gcactacaac gcttacaatc ctgagacaaa gaaggcacac ctttcgccta
     2701 aagagaaaca tgaaataact aaaaagataa gcgcccttgc ggagcgctat ctttctctgg
     2761 ttacctagaa gcagtaccta cgctgcgtct ttttcttcct cggttctcgc aacctttcta
     2821 ctccgtatgg tgcggaactc atcctcgtac ttccctatat tagcgtccgt ttcttcttct
     2881 atatcgatga gctcgttacg tgcaagatca atggagcgaa taatctcgtc gagcttcagg
     2941 tgtatcgcgt gggcgtcgcg gttctgagtg ttctgaatga gaaacaccat gaggaaagta
     3001 aggaccgtcg tgccggtgtt gatgatgagc tgccaggtat ctgagtagtt gaagtaggga
     3061 ccggtgactg cccaaagcac gacgatgcca cacgagacaa tgaacgctga tggggttccc
     3121 atcagagtac tcgtatagtg ggcaaccttc ctgaaaagtt cgttcatgga acgactactg
     3181 catcttcccg cagcacgcac acttatggcc ggcgagctta ctcaacccag caacaatgag
     3241 aagtactggc cacacaattg caataatatc gccatcgacg atatcaaggg cacggagcaa
     3301 gaatgcgaga ccgataagca caatcatgag cggaacaagc ttgtgatgat ggcaggagca
     3361 ggtgccggat gcgttcatag aaacgggtaa ttaccttata tgcctaaagc gtagcacctc
     3421 tcagctgaag cgttcctgaa gcagtggata gctattctac ggtcaccgac ttagccagat
     3481 tccgaggttg gtcgatggga agaccccgct caagcgccac atagtaggca aagagctgga
     3541 cgggcatagc ggtaagaata gggaggaggg cttcatgggt agggggtact gaaatgacgt
     3601 cgtcagcaag acccgtaagg tcatcctcgt gcccgtcggt agtgatggcg atgactttgc
     3661 cgccgcgtgc gcgtatctcc tgaatattag acttcgactt ctcatacatg gagtcgcccg
     3721 ggaccaggac cacagtgagg aatgatggct cgatgagtgc gatagggcca tgcttcatct
     3781 cacctgaggc gttgccttca gcatgcacgt acgcaagctc cttcagtttt atcgcaccct
     3841 cataggcaac ggggcactga tatttccgtc ctaaataaaa cgcattctcc accgctacat
     3901 acttcttcgc tatcgcttgg atatggtcgc ggcgggacag tatctcgcgg gcaagggcag
     3961 ggaggcgatt tagttcagca acaaatgatt gtccgtcggt agtagagagt cctttctggc
     4021 gacctaaggt cagcgcgata agcgcaagga cggtcatctg tgaaatcact gctttcgttg
     4081 aggcgacgct tatttcaggg cccgcatggt tatacacgcc cgcatcggtt tctcgtgcga
     4141 tagaagagcc cacaacattc actattccta aggtcatcac gccgtgacgc ttcgcttcgc
     4201 gtatggcttc aatagtgtcg agcgtttctc ctgactgaga caccgcaaga aggagcgtct
     4261 cttcgggatg catggtgtta cgcctatacc gaagttcaga gccaagctcc acctctaccg
     4321 gaataccggc gaactcttca atgagaagtt caccgacctt ccc
//
LOCUS       DAORIN010000048         4328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4227_length_4328_cov_5.711912, whole genome shotgun sequence.
ACCESSION   DAORIN010000048 DAORIN010000000
VERSION     DAORIN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4227_length_4328_cov_5.711912"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PK109_02450"
     CDS             <1..184
                     /locus_tag="PK109_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRH24422.1"
                     /translation="GVVALDEVDRVVTDPRNQRTSVEGIWAAGDCTDELYHQNNIAAG
                     DAVKALEDIYQWIKTR"
     gene            complement(214..1179)
                     /locus_tag="PK109_02455"
     CDS             complement(214..1179)
                     /locus_tag="PK109_02455"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRH24423.1"
                     /translation="MNTNGMGKVQVFATNAGKELGTKISQVLGTNLGEARLARFKDGE
                     VDVQILENVRGNDVFIVAPTQPPAENLLEAVHMAEAARLSSAARVTLVIPYFGYGRAD
                     RKDAPRKAIGARLAFKLLEIAHPDRFLMLDLHAEQTLASVDNAVTDHLYGSAVGVPYL
                     KKLLDGKDFVIASPDRGGGARAIKYATLLGHDDFVFFSKQRSAPGEVKSDSIKIIGDV
                     KDKLVVLVDDMIDSGGTIVADAAAAKAEGASGVWAFATHGLFSGGALEKFKASAIDRV
                     FTTDSVYHDPEELKAAKVEVISVASLLADAIKRTHDGESLSTLIS"
     gene            complement(1191..2423)
                     /locus_tag="PK109_02460"
     CDS             complement(1191..2423)
                     /locus_tag="PK109_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24424.1"
                     /translation="MALPKAAACKECGTDPVHHRLTYITVALDETLRPIFAPGPFMRF
                     IGKGVYALERVVSPRLLQLFVKLGWAKAVAAPDDDTMLLAKMLWEEAGKRGIEVREIR
                     LGGLARNLFLAKYPDGRVIAYEGIPLPPSGLAQAPWMDNKAEMKRRFRTLGIPVAAGG
                     AAFTEHGARSVFQKVVPPVIVKPYSGSASRHTNLHITNELKLFRAFHSAKKVAPLALI
                     EEELVGPVFRATVVDGKFAAALRRDPPHVIGDGMHTVEQLIEEANKNPQREGPYFSKM
                     QITAETLLELKWQNLTPESIPEKGRRVTLHQKVNWSLGGTTADVTDEVHADNVALFEQ
                     VAHVLKAPIVGMDVIIGDMSRSWRDQERCGIIECNSMPFFDNHHLPFEGKPRNVAGLI
                     WDMVEPKNKQRAGETEGV"
     gene            complement(2435..3850)
                     /gene="purF"
                     /locus_tag="PK109_02465"
     CDS             complement(2435..3850)
                     /gene="purF"
                     /locus_tag="PK109_02465"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRH24425.1"
                     /translation="MRSLNEKCAVFGIYGKDIDVARLSFFGLFALQHRGQEGSGIAVS
                     DGETIRYHKDHGLVAQVFTEEAISSLNGYMAVGHNRYSTSKGSGTEHVQPMIVGDLAF
                     VHNGNLPSTKALEVFLSDKGVEVTNLTDSCLMAHAISWHMGQGMTLADAVVASYPLFT
                     GAFSIIAMTKTEMVAARDPKGIRPLTLARLQGGFVVTSETCALGTVGATFERDIEPGE
                     MILINQDGITATQVAPGDPKLDIFEFIYFARHDSELMGKSVYEVRKNFGKTLAREYPV
                     DADVVIGVPETSLPCALGYAEQSGIPYEMGLNKNRYIQRTFIQPDQKLRDKSVKMKLT
                     PIPGVLKGKRVVVIDDSIVRGTTSKPIVELLFEAGAKEVHFLVSSPPIRFPDFYGIDT
                     PAQSNLLAYGKTVEEMRQYLGATSLYFLSVDGMLASTGLPKDHFNTSPFTGEYPIDLK
                     ERYEEFNYPSKVEGVPVAEKI"
     gene            complement(3874..4122)
                     /locus_tag="PK109_02470"
     CDS             complement(3874..4122)
                     /locus_tag="PK109_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRH24426.1"
                     /translation="MDDLQEIPGVGKSIAEDLRLIGISSVHSLKGKDPERLYAKHNKR
                     VGRIEDRCLLYTFRYAVYYAEGGRNAQKLKWWNWKDQK"
BASE COUNT         1012 a         1132 c         1126 g         1058 t
ORIGIN      
        1 aggcgtggtc gcgcttgatg aagtagatcg tgtggtgacc gatccccgca atcagcgcac
       61 gtctgtagaa ggtatatggg ccgctggtga ctgcactgac gagctctacc accagaacaa
      121 catcgccgca ggagacgccg taaaggcgct tgaggacatc taccagtgga ttaagactcg
      181 ctagttttta ctaaaacagg caggggagtc gcattaggaa attaacgttg agagcgactc
      241 accgtcgtgc gtcctcttta ttgcgtccgc taaaagggaa gccacgctga tcacttctac
      301 cttagcagcc ttgagctctt cagggtcgtg atacacggag tcagttgtaa agacgcggtc
      361 tatggcgctt gctttgaact tctctaaagc acctccagag aagagaccat gagtcgcaaa
      421 tgcccaaact cctgaggcac cctcagcttt tgccgctgca gcgtctgcaa caatggtgcc
      481 accgctatca atcatgtcgt caacgagtac cacgagtttg tcttttacgt cgccaattat
      541 ctttatagaa tcactcttca cttcgcctgg ggcagagcgc tgtttggaga agaatacgaa
      601 gtcatcgtgg ccaagcaagg ttgcgtactt aatcgcacgc gcgccgccgc cacggtcagg
      661 acttgctatg acgaagtcct tgccatcgag gagtttcttg aggtacggca ccccaacggc
      721 tgagccgtac aaatggtccg tcactgcgtt atccacagat gccaacgtct gctctgcatg
      781 gaggtcgagc atgaggaatc ggtcagggtg agcgatttcg agaagcttaa aggcaagacg
      841 ggcgccaatg gccttccggg gcgcatcttt gcgatctgct cgtccgtagc cgaaataggg
      901 gatgacgagc gtgacgcgtg cggcggacga aagacgtgcc gcctcagcca tatgcaccgc
      961 ctcaaggaga ttttctgctg gcggctgggt gggagccacg atgaaaacgt cgtttccacg
     1021 gacattctca agaatttgca cgtctacttc cccatcctta aaacgcgcta atctcgcctc
     1081 gccgagattt gtacctaaca cttgactaat cttagttcca agctcttttc ctgcgttcgt
     1141 tgcaaagacc tgcacttttc ccatgccgtt agtgttcata gatagttgga ttatacgccc
     1201 tctgtctccc ctgctctttg cttatttttt ggctcgacca tgtcccagat gaggccggcc
     1261 acattgcgag gttttccttc aaatggcaga tggtgattat caaagaacgg catgctgttg
     1321 cattcgatga tgccgcagcg ttcctgatcg cgccatgaac gactcatatc accgataatg
     1381 acgtccatac cgactatggg cgccttaagg acgtgtgcta cttgctcaaa gagggccaca
     1441 ttatctgcat gcacctcgtc agtgacgtca gcggtggtgc cgccaaggct ccagttcacc
     1501 ttctgatgga gtgtcacccg tctccccttc tctgggatac tttctggcgt aaggttctgc
     1561 catttgagtt caagaagcgt ttcagcagtt atctgcatct ttgagaagta tggaccttca
     1621 cgctgcggat tcttgtttgc ttcttcaatg agttgttcaa cggtatgcat cccatctcct
     1681 atgacatgcg gcgggtcgcg gcgaagtgct gctgcaaact tcccatccac cacggttgcg
     1741 cggaacacag ggccgacgag ttcttcttca attaatgcga gcggcgctac ctttttagcg
     1801 ctatgaaatg cgcggaagag ttttagctca ttagttatat gaagattggt gtgacgcgag
     1861 gcagagccag agtacggctt cacaatgacg ggaggtacta ccttttgaaa cacgctacga
     1921 gcaccatgct cagtgaacgc agcgccgccg gcagcaaccg gaataccgag cgtacggaac
     1981 cgtcgtttca tctctgcctt gttgtccatc catggtgcct gcgcaagacc agacggaggt
     2041 aacggtatac cttcatacgc gataacccga ccatcgggat attttgcaag gaacaggtta
     2101 cgcgcgagtc caccaagacg aatctcgcgc acttctatac cccgcttccc tgcctcttcc
     2161 cagagcatct tcgccaaaag catcgtgtca tcgtcaggag cggcaactgc tttagcccac
     2221 ccgagtttta caaagagttg gagtaggcgc gggctcacaa cgcgctcaag cgcgtacacg
     2281 cccttcccga taaatcgcat gaacggtccg ggagcgaata tcggacggag cgtctcatcg
     2341 agcgcgacgg tgatatacgt taaccgatga tggacaggat cagtgccaca ctctttgcac
     2401 gccgcagctt tcggaagcgc catagaacga tgcgttatat cttttcagca accggaaccc
     2461 cttctacctt tgaagggtaa ttaaactctt cataccgttc tttcaaatct atcggatatt
     2521 cccccgtaaa cggagaggta ttgaaatgat ctttcggaag acctgtggaa gccagcatac
     2581 cgtcgacaga aaggaagtag aggctcgtcg cgccgagata ctgccgcatc tcctctaccg
     2641 tctttccata ggcaagcagg tttgattgtg cgggagtgtc gatgccatag aaatcaggga
     2701 aacgaatagg aggagaagag accaggaagt gcacttcctt tgcccccgct tcaaagagaa
     2761 gttcgacgat aggttttgag gtcgtgcctc ggacaataga gtcgtcgata accacgaccc
     2821 gcttcccttt taatacgccc gggataggcg taagtttcat ctttacgctt ttatcgcgca
     2881 gtttctgatc tggctgaatg aaggtgcgtt gaatgtagcg attcttgttg agtcccatct
     2941 catacggaat accggactgc tcagcgtatc ccaaagcgca tgggaggctc gtctcaggca
     3001 caccgataac tacgtcagcg tcgactggat attcacgcgc caaagtctta ccgaagttct
     3061 tacgcacttc gtataccgat ttacccatca gctcactgtc atggcgtgcg aaataaataa
     3121 actcgaatat atcgagtttc gggtcgccag gcgctacctg tgttgcagta ataccgtctt
     3181 ggttaatgag aatcatttct ccaggctcaa tatcacgttc gaacgttgca ccgacagtgc
     3241 caagcgcgca ggtctcgctc gtaacgacaa acccgccttg gaggcgggca agcgtgagtg
     3301 ggcggatgcc ctttggatcg cgagcggcga ccatctccgt cttcgtcata gcaatgatgg
     3361 agaaagcgcc agtgaaaaga ggataggacg cgaccacagc atcagcgagt gtcatcccct
     3421 gccccatatg ccaggaaatg gcgtgtgcca taaggcagga gtcagtgagg ttcgttactt
     3481 cgactccttt atcactaaga aatacttcaa gtgctttggt tgagggaaga tttccattat
     3541 gaacaaacgc aagatcgccc acgatcatcg gctgcacgtg ttcggtacca cttccctttg
     3601 aggtggagta gcggttatga ccgacggcca tatacccgtt aagcgatgag attgcttctt
     3661 cagtaaatac ttgggccacg agtccgtggt ccttgtgata gcgaatggtt tctccgtctg
     3721 agaccgcaat accagaacct tcctgaccgc gatgctggag tgcgaagagt ccgaagaatg
     3781 agaggcgggc gacatcaatg tctttgccgt agatgccgaa gactgcgcat ttttcgttga
     3841 ggctccgcat gattcattta tcttatcaca gccctatttt tggtccttcc agttccacca
     3901 cttgagcttt tgggcattac gcccgccttc ggcataatag acggcgtagc ggaatgtata
     3961 gagaagacag cggtcctcaa tacgccctac ccgcttgtta tgtttggcat acagcctttc
     4021 tgggtctttc cctttaagag aatgtacgga cgatatgccg atgaggcgca gatcttcggc
     4081 gatagatttc cctacgcccg gaatttcctg tagatcatcc atagaatgcg gtgtttgttg
     4141 gacaaagttc gaaactattt cgagcaaaat ccaaatgatt aaaaatacta atgcggacga
     4201 ggcagggcga aacaatttgt ctgggggaat ggattcgccc tgcctcgact gatttcccgc
     4261 ccgcaggagg ggtctgggga ggaatgcggg tgggtttcgg actgggggtt ttcggaaatt
     4321 cggcggca
//
LOCUS       DAORIN010000049         4246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4359_length_4246_cov_3.980196, whole genome shotgun sequence.
ACCESSION   DAORIN010000049 DAORIN010000000
VERSION     DAORIN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4359_length_4246_cov_3.980196"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..680
                     /locus_tag="PK109_02475"
     CDS             69..680
                     /locus_tag="PK109_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRH24427.1"
                     /translation="MTDTMTTDTEVKDAAPVAPETADAAAVPRDGARGSQRGRGRDGG
                     GRPGGRGRERAPRERSEFDSVTVDVRRVARVMAGGRRFSFSVTIVIGDRKGRVGVGIG
                     KAADTALAIDKATRDAKRHLITVPLTKSNSIKHAISANYASSVLTIVPSPGRGLVAGS
                     AMRTVLEFAGVTDVVGKILSRTKNKLTIARATVVALKSLRTSA"
     gene            686..1135
                     /locus_tag="PK109_02480"
     CDS             686..1135
                     /locus_tag="PK109_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HRH24428.1"
                     /translation="MQSNTLKARTPRKTTDRVGRGGKRGKTSGHGIKGQKGRSGRRMR
                     PEMRDLVKKLPKLRGHGKNRSKTVRAGRTSFRPVNLATIEHHFAAGDTVTPATLLKKG
                     IISMRGGKMPAVKILGTGELTKKLAFSDVAVSAPAKAAIEKAGGSAL"
     gene            1139..2413
                     /gene="secY"
                     /locus_tag="PK109_02485"
     CDS             1139..2413
                     /gene="secY"
                     /locus_tag="PK109_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRH24429.1"
                     /translation="MINAAVHKLKVIFGDSVLRTRILFIFAAFAVFRLLAAIPIPGVD
                     EVRLQQFFANNQFFGLLSIFSGGGFATLSLVMLGVGPYITASIIMQLATVIFPQVKRM
                     YQEDGEAGRTKFIAISRMLTIPLAVLQGVAFLSLLESQGIIGTLTSFEFAVNVFLVAA
                     GSMLLMWIGELITEFGIGNGVSLIIFAGIVSQIPAGISQLAFTATAANIPAYLGFFIL
                     AIAAIYAVVTASEAERSIPIAYARQVRGSVTTGGAATYLPIRILQAGVIPIIFALSLL
                     LLPQMVLSALTAIGVTGLAGAAAAYTAFSANPWAHGAVYFVLVVMFTYFYTSITFEPH
                     RVAENLQKSGAFIPGVRPGRETEAYIGAVVNRITLPGAFFLGAIAVIPFVVQGLTGIT
                     AILIGGTALLIGVTVLLDLIRKIDAQASLREY"
     gene            2444..3028
                     /locus_tag="PK109_02490"
     CDS             2444..3028
                     /locus_tag="PK109_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24430.1"
                     /translation="MRGKLILIVGSTGSGKGTLMRHAMAQFPEIQTPQSYTSRPRRAD
                     AIENTHYTFITTEAFKEKIAQGEFLEWAEFSGNYYGTLKQDIEEGLTHGALMLKEMEV
                     QGVRQVREILAKDDLLIVFVDAGSWEELAKRAMAREPMDAATLEKRRLRYEDELTFMP
                     EADVVIKNLPGKQEEAAAAFEAVIAKAMEETHHG"
     gene            3030..3602
                     /locus_tag="PK109_02495"
     CDS             3030..3602
                     /locus_tag="PK109_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRH24431.1"
                     /translation="MNYKTVLLMGLPGSGKGTQAKLLAERLGWKHFSTGDVFKELRNG
                     TGPLAEKVKETYDNGRYAPDWFATYLFEDTMLNLPADQGVVCDGYPRTLAQAEIFDDT
                     LDWLGRPYLALNLAVREDEALRRQIERAKVEHRPDSATPEQVKIRFEQYQANTEPLLN
                     YFREKGVLGEINGEETPEEVAAAIAAALQQ"
     gene            3599..3802
                     /locus_tag="PK109_02500"
     CDS             3599..3802
                     /locus_tag="PK109_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24432.1"
                     /translation="MIVRTLEEREGIIESGKRLGAILDQVAAAAKVGVSTATLDALAE
                     KLIRDGGDEPAFLGYRPDRATRK"
     gene            3824..>4246
                     /locus_tag="PK109_02505"
     CDS             3824..>4246
                     /locus_tag="PK109_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRH24433.1"
                     /translation="MNDEVVHGIPNENPRILQDGDIVALDIGLAHKGFITDTAVTVAI
                     GRIDAETSQLLDVTKRSLEEGIKAARPGNRVGDISHAIESAFEGSGFSIVKVLGGHGV
                     GTHVHEEPWIGNVGHPGTGEELVAGMGLALEAIAKAGKA"
BASE COUNT          915 a         1202 c         1169 g          960 t
ORIGIN      
        1 ctatgcctat gctgcacaga taaagtcgct tgccgacgcc gcacgcgagg gcgggcttac
       61 tttctaatat gactgacacg atgacgacag acactgaagt gaaggacgca gcacccgttg
      121 ctcccgagac tgctgacgca gcagcagtgc cgcgcgacgg tgctcgtggt tctcagcgtg
      181 gccgtggtcg cgatggcgga ggccgtccag gtggccgtgg ccgtgagcgc gctcctcgtg
      241 aacgcagcga gttcgactcg gttaccgtcg acgtacgtcg cgtcgctcgt gttatggccg
      301 gaggtcgccg cttctccttc agcgtcacca tcgttatcgg tgaccgtaag ggacgcgttg
      361 gcgtcggtat tggtaaggct gctgacaccg cgcttgctat tgataaggca acgcgtgacg
      421 caaagcgcca tctcatcacc gttcctctca cgaagtcgaa ctccatcaag cacgcaattt
      481 ctgcaaacta cgcatcatcc gtactcacca ttgttccttc tccaggacgc ggtcttgttg
      541 cgggttccgc aatgcgcacc gtccttgagt ttgcgggcgt taccgacgtt gttggtaaga
      601 ttctttctcg cacgaagaac aagctcacca ttgctcgcgc aacggtagtt gcccttaaga
      661 gcctccgcac ttctgcctaa ctgttatgca atcaaacact ctcaaagcac gcacccctcg
      721 taagaccacc gatcgcgttg gacgcggtgg taagcgcgga aagacctcag gtcacggcat
      781 caaaggccag aagggtcgct caggacgccg catgcgccct gaaatgcgcg acctggttaa
      841 gaagctcccg aagctccgtg gtcatggtaa gaaccgctcg aagaccgtac gtgcaggacg
      901 cacctcgttc cgccccgtta accttgctac catcgagcat cacttcgcag caggggacac
      961 cgtcacgcct gcaactcttc tcaagaaggg catcatctcg atgcgtggcg gtaagatgcc
     1021 ggcagtaaag attcttggta ccggtgaact caccaaaaag ctcgcgttct ctgacgtagc
     1081 agtatccgct cccgcaaagg cagctattga gaaagcaggc ggatccgccc tctaatcaat
     1141 gatcaatgca gccgtacaca agctcaaggt aatcttcgga gactcggtgc tccgcacgcg
     1201 cattcttttc atattcgcgg cttttgcggt attccgtctt cttgcagcta tcccgattcc
     1261 gggcgttgat gaggtacgcc tccagcagtt cttcgcgaac aaccagttct tcggacttct
     1321 ctctatcttc tcgggcggcg gctttgcgac gctctcactc gtgatgcttg gtgtggggcc
     1381 gtacatcact gcatccatca tcatgcagct tgctacggtt atcttcccgc aggtaaagcg
     1441 catgtaccag gaggacgggg aagcaggacg cacgaagttc atcgctatct cgcgcatgct
     1501 caccatcccg cttgcggtac ttcagggcgt tgccttcctc tcacttcttg agagccaggg
     1561 catcattggt acgctcacgt cgtttgagtt tgccgtaaac gtattcttgg tggctgctgg
     1621 ctccatgctg cttatgtgga tcggtgagct catcactgag tttggtattg gtaacggcgt
     1681 gtccctcatc atcttcgcgg gtatcgtttc ccaaattcct gccggtatct cgcagcttgc
     1741 cttcaccgca acggcagcaa acattccggc ctaccttggc ttcttcatcc tcgctattgc
     1801 cgctatttac gcagtggtta cggcgtctga agcagagcgc tctataccga ttgcgtacgc
     1861 acgccaggta cgcggaagcg ttactaccgg cggcgctgcc acttatcttc ctatccgcat
     1921 ccttcaggcg ggcgttattc cgattatctt cgcactctcc ctccttcttc ttccacagat
     1981 ggtcctttcc gcacttaccg ctattggtgt caccggcctt gcgggcgccg ccgccgccta
     2041 cactgcgttc tctgcaaacc catgggcaca cggtgccgtc tacttcgtgc tcgtcgtgat
     2101 gttcacctac ttctacactt ctattacgtt tgaaccacat cgcgttgcag agaatcttca
     2161 gaagtcaggc gcctttattc cgggcgttcg tccaggacgc gagacggaag cctacattgg
     2221 tgcggtggtg aaccgcatca cgctccctgg cgccttcttc ctcggtgcta tcgcagtcat
     2281 tccgttcgtg gtccagggtc ttaccggcat cacggcaatc cttatcggtg gtaccgcact
     2341 ccttatcggt gtgaccgtac ttctcgacct catccgcaag attgatgcac aggcatcact
     2401 gcgcgagtac taattgcgcc gggcgtgcta gatttctagc atcatgagag gaaaacttat
     2461 tcttattgtt ggctctaccg gaagcggtaa gggtaccttg atgcgtcatg cgatggcgca
     2521 gtttcccgag atacaaacgc cgcaatccta tacgtcacgc cctcgacgtg ctgatgctat
     2581 tgaaaacacg cactacacct tcattaccac cgaagcgttt aaagagaaaa tagcgcaggg
     2641 tgagtttctt gagtgggctg agttcagtgg gaactactac ggcacgctca aacaggatat
     2701 cgaagaaggg cttactcacg gcgcgctcat gctgaaagaa atggaggtgc agggagtgcg
     2761 tcaggtaaga gagattctcg caaaagacga ccttctcatt gtctttgtcg atgcgggttc
     2821 gtgggaggaa ctcgccaagc gcgcgatggc acgagagccg atggatgcgg caacgcttga
     2881 aaagcgtcgc cttcgctacg aagacgagct cacgttcatg ccagaagctg atgtggtcat
     2941 aaagaacctt ccgggaaagc aagaggaagc agcagcagcg tttgaggcag tgattgcaaa
     3001 agcgatggaa gaaacgcatc atgggtaata tgaactacaa gaccgtacta ctcatgggcc
     3061 tcccaggctc aggaaagggc actcaagcaa agcttcttgc ggagcgtctc ggctggaagc
     3121 atttctccac gggagacgtg tttaaggaac tccgtaatgg tacgggtcct cttgctgaga
     3181 aagtgaagga aacctatgac aatggtcgct acgctcctga ctggttcgct acctatctct
     3241 ttgaagacac tatgctgaac ctcccggcag atcagggggt ggtgtgcgac gggtacccgc
     3301 gcacacttgc gcaggcggag atatttgatg acacgctcga ctggctcgga cgcccgtacc
     3361 ttgcgcttaa ccttgcggtt cgcgaagacg aagcacttcg tcgtcagata gagcgggcaa
     3421 aggtggagca tcgccccgat agcgcaacgc ccgagcaagt caaaatacgc tttgagcagt
     3481 atcaggcaaa taccgaaccg ctcctcaact atttccgcga gaagggagta ctgggagaga
     3541 taaacggaga agagactcca gaagaggttg ctgcagctat cgccgctgca ctacagcaat
     3601 gatcgtacgt acccttgagg aacgcgaagg catcatcgaa tcaggcaagc gccttggcgc
     3661 catccttgat caggtagccg ctgccgcaaa ggtaggggtc tccaccgcca ccttggatgc
     3721 gcttgctgaa aaactcatac gcgacggcgg ggacgagcca gcgttccttg ggtatcgccc
     3781 ggaccgtgcg acgagaaaat agcctgcaac actctgtgtt tctataaatg atgaggtggt
     3841 gcacggcatc ccgaacgaga acccgcgcat actccaggat ggcgacattg tcgcgctcga
     3901 tatcggtctt gctcataaag gatttattac ggataccgca gtcaccgttg ctattggacg
     3961 cattgatgcc gagacgtctc aactcctcga cgtcacgaaa cgttcgcttg aagaagggat
     4021 aaaggcggcg cgtcccggga accgtgtcgg tgacatctcg cacgccattg aatcagcgtt
     4081 tgaaggttct gggttctcca tcgtgaaggt gctcggagga cacggggtag gcactcacgt
     4141 gcacgaggag ccctggatag ggaatgtggg gcacccgggg accggtgagg agcttgtggc
     4201 aggtatgggg cttgcccttg aggctatcgc aaaagccggc aaggct
//
LOCUS       DAORIN010000050         4211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4416_length_4211_cov_5.116939, whole genome shotgun sequence.
ACCESSION   DAORIN010000050 DAORIN010000000
VERSION     DAORIN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4416_length_4211_cov_5.116939"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PK109_02510"
     CDS             complement(<1..1087)
                     /locus_tag="PK109_02510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRH24434.1"
                     /translation="MPHRAPKRVLIFSLAYRPFIGGVEVAIQEITNRIPFEEIEFHVL
                     TLRMDSRLPREERLGNVVVHRIGFARSGAPITDLTRFPLRLNLYLYQLWAAFAAAQLH
                     AKYKFDLVWAMMAHTAGIPAGIFKRFHPSVPYLLTLQEGDPPEYIEKLMRPVFSLFKQ
                     GFTQANELQAISGFLLAWGKRMGFRGTGVLIPNGVDTKRFARTHARTADGTTRLITVS
                     RLVRKNAVDDIIRALALLPDSVSLSIAGIGSDEKMLRALVEEQGLTSRVTFLGNVSQE
                     ALPELLSSHDIFVRPSRSEGMGISFIEAMAAGLPVIATQEGGIADFLFDGKRNPELPT
                     TGFAVDTDAPDQIADAVMRVLAHPEETE"
     gene            complement(1068..1919)
                     /locus_tag="PK109_02515"
     CDS             complement(1068..1919)
                     /locus_tag="PK109_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRH24435.1"
                     /translation="MGKLILVTGGAGFVGSNLCRRLQAEGHTVISLDNYFTGSKENHV
                     PGVEYREGHTKDIAALVPETPDLVFHLGEYSRVEKSLEEPELVWDLNKVGTLGVLEFC
                     RMKQVKIVYAGSSTKFGDGGLGRDQSPYAWAKATNTELVKNYGTWYGLPYAITYFYNV
                     YGPGERAGTYGTVIEIFRQKMRLGEPLGVNAPGTQKRNFTHVDDIVDGLLLVGENGSG
                     DDYGIGDEHSYSILEVAEMFGGAIEMRPEVQGNRMGSDIDTTKTRSLGWEPKQHLPDY
                     IHATQSA"
     gene            complement(1966..3663)
                     /locus_tag="PK109_02520"
     CDS             complement(1966..3663)
                     /locus_tag="PK109_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HRH24436.1"
                     /translation="MDNPERVTYFAATDTRGKRIPFGIKQKDRARHMYIIGKTGMGKS
                     TMLENMAIQDIRNGEGLAFIDPHGGTVDKLLDYIPENRIKDVVYFAPFDLEHPIAFNI
                     MEDVGYDKRHLVVSGLMAAFKKIWVDAWSARMEYILTNTLLALLEYPDATLLGVNRMY
                     TDKNYRKKVVENVKDPVVKDFWIKEFATYTDRYTQEATPAIQNKIGQFTSNPLIRNII
                     GQPTSSFDIRTLMDQKKILIINLSKGLVGDTNTQLLGSMLTTRIFLAAMSRADASARD
                     LARLPNFYFYVDEFQNFANETFAEILSEARKYKLNLIIAHQYIEQMEEEVRDAVFGNV
                     GTTVTFRVGPFDAETLETVFAPEFEQEDLVNLGFAQIYTTLMIDGIGSRPFSAVTIPP
                     IEPPPISTRKEVLASSRATYAALRTDIEEAILKELSLTEEEVTRKLDKMDAKLDDRGY
                     KLPPNKKPKQKQYQQPSPQKAPPQNPPGGAVHAQDLKAAIAAITQKTQATQQEKTETK
                     HEALKETLQKVAAPQPAPLPPKPHPAPQPPVADIPPNKRPAFEVPEESLQAVFEEDL"
BASE COUNT          970 a         1067 c         1108 g         1066 t
ORIGIN      
        1 tctctgtttc ttcagggtgc gcgagcacgc gcatgacggc atcggcgatt tgatcgggcg
       61 cgtctgtatc aacggcaaac cccgttgttg gtagttcggg attacgtttt ccatcaaaaa
      121 gaaaatcagc aataccgcct tcttgagtag cgatcaccgg aagtcctgcg gccatcgctt
      181 caataaaaga aatacccata ccttctgaac gcgacgggcg tacaaatata tcgtgtgaag
      241 acaagagttc aggtaatgct tcctgagaga cattcccaag gaacgtaacg cgtgaggtaa
      301 gcccctgctc ttccaccaga gcgcgaagca ttttttcatc agaccctatc ccagcgatag
      361 aaagcgatac agagtcagga aggagtgcga gtgcacgaat aatgtcatca accgcgttct
      421 tacgcacaag acgagaaact gttatgaggc gcgtcgtgcc gtctgccgta cgcgcatggg
      481 tgcgtgcaaa acgtttcgta tcaacaccgt tcgggatgag tactccagtg ccacggaacc
      541 ccatgcgttt tccccacgca agcaagaatc cagatatcgc ttggagttca ttggcttgtg
      601 tgaatccttg tttgaaaagt gagaacaccg gacgcatcaa cttttcaatg tattccggtg
      661 gatccccttc ttgaagcgtt aaaagatatg gaacggaagg atggaaacgt ttaaagatgc
      721 ctgcaggaat acctgccgtg tgtgccatca tggcccacac gagatcaaat ttatacttcg
      781 catgaagctg tgctgcggca aacgcagccc agagttgata gaggtacaag ttaagacgaa
      841 gaggaaaccg cgtgagatca gtgatgggag ccccggagcg cgcaaacccg atacggtgca
      901 ccacgacgtt tccgagtcgc tcttcccgcg ggagtcgtga gtccatgcga agcgtgagca
      961 catggaattc aatttcctca aaaggaatgc ggttggtgat ttcttgtatc gctacttcaa
     1021 cgccgcctat aaacgggcgg tacgcaagcg aaaaaataag aacccgctta ggcgctctgt
     1081 gtggcatgaa tgtagtcagg aagatgctgc tttggctccc accccagtga acgcgtcttg
     1141 gtcgtatcaa tatcagaccc catgcgattt ccttgaacct caggacgcat ttcaatagcg
     1201 ccaccaaaca tctcggcaac ctcaagaatt gaatacgagt gctcgtcccc gatgccatag
     1261 tcgtcaccgc ttccgttttc tccgactaag agaagtccat caacgatgtc atcgacatgc
     1321 gtgaagttac gcttctgcgt tccaggagca ttcacgccaa gcggttctcc taagcgcatc
     1381 ttctgacgga atatttcgat aacggtgccg tacgtgccag cacgctcccc aggaccgtat
     1441 acgttataga aataggtaat ggcatacggg agtccatacc aggtgccgta gtttttgacg
     1501 agctcggtat tcgtcgcctt cgcccaagca tatggactct gatcacgtcc aagtccgccg
     1561 tcaccaaact tagtagaaga accggcatac acgatcttca cctgcttcat gcggcaaaac
     1621 tcaagtaccc caagcgtccc taccttattg aggtcccaca ctagttcagg ttcttcaaga
     1681 cttttctcaa cgcgagagta ttcacccagg tgaaagacga ggtctggggt ttcaggcacc
     1741 agcgcggcga tatctttagt gtgcccttcg cgatattcca cgcccgggac atggttctcc
     1801 ttgcttccag tgaaatagtt atcaagggat atcaccgtgt gcccttcagc ttgtagcctc
     1861 cggcagagat tggatcctac aaagcccgcg cccccggtaa cgagtatgag tttgcccata
     1921 taggcctagg ataggctacg gagggccatt tccgcaaata gaccgctaaa gatcttcctc
     1981 gaaaactgct tggagacttt cttcgggaac ttcaaacgct ggacgcttat tgggcggaat
     2041 atccgctacc ggaggctgcg gcgccgggtg cggctttggc ggaagaggtg ctggctgcgg
     2101 tgctgcgact ttctgtagtg tttctttcag ggcttcgtgc ttggtttctg ttttctcttg
     2161 ctgcgtcgct tgcgttttct gcgtgattgc cgctatcgca gctttcaagt cctgggcgtg
     2221 aacggctcct cccggaggat tctgtggagg tgctttttgt ggactcggtt gttgatactg
     2281 cttttgtttt ggcttcttgt tgggcggaag tttgtatccg cgatcatcaa gtttcgcgtc
     2341 catcttatcg agcttacgtg tgacttcttc ttccgttaac gaaagttctt tgagaatcgc
     2401 ttcctcgatg tcggtgcgga gcgccgcata ggtggcacgt gaggaagcaa ggacttcttt
     2461 acgagtagat atgggcggtg gctcaatagg aggaatggtg accgcggaga agggacgcga
     2521 accaatacca tcaatcatca aggtcgtgta gatctgtgcg aacccgagat tcacgagatc
     2581 ttcctgctca aattcaggcg cgaatacggt ctcgagtgtc tctgcatcaa aaggaccgac
     2641 gcggaacgtt accgttgtgc ccacgtttcc gaacacagca tcgcgtacct cttcttccat
     2701 ctgctcaatg tactgatgcg cgatgatgag gttcagtttg tacttacgtg cttcagaaag
     2761 tatttctgca aaggtttcgt tcgcaaagtt ctggaactcg tccacgtaga agtagaagtt
     2821 cggcaaccgt gccagatcac gggcagaggc gtcggctcgc gacattgccg caaggaagat
     2881 gcgggtcgtg agcatggatc ccaacagctg ggtattagtg tcgcctacga gaccctttga
     2941 gaggttgatg atcagaatct tcttctgatc catgagcgta cgtatatcaa aggaagacgt
     3001 tggctggccg atgatattgc gtatcaacgg gtttgaggtg aactgtccga tcttgttttg
     3061 aatggccggt gttgcctctt gtgtgtagcg gtcagtgtag gtcgcaaact cttttatcca
     3121 gaaatcctta acaacgggat ccttcacgtt ttccactacc ttctttcgat agttcttgtc
     3181 cgtatacatg cggttcacgc caagcaaggt tgcgtctgga tactcaagaa gtgcgagaag
     3241 cgtgttcgtg agaatgtact ccatgcgcgc gctccaggcg tctacccaga ttttcttgaa
     3301 tgccgccatg aggcccgaca cgacgaggtg gcgtttgtcg tagccgacat cctccatgat
     3361 gttaaacgca atggggtgct ccagatcaaa gggagcgaag tacaccacat ccttgatgcg
     3421 gttctcaggg atatagtcca gcagcttatc aaccgtgccg ccgtgcggat cgatgaaggc
     3481 gagcccttcc ccgttgcgga tatcttgaat cgccatgttc tcaagcatgg tcgatttacc
     3541 cataccggtc ttaccaatga tgtacatgtg gcgcgcacga tccttttgct taataccaaa
     3601 cggaatacgc ttaccgcgcg tgtcggttgc cgcaaagtac gttacccgtt ccgggttatc
     3661 cataggcata gtatacgcca cgaaaagact gaaggcagcg agtatgtgct cgctgccctt
     3721 ctcgtcctga cctatggtcg cgacgttgtt tccgcgaaaa cgtgtgctgc gtaccgaagt
     3781 agcgcaaacc cgtttggatc ggaaaattct tctggtgtta ggctgccgct gcgtatgcga
     3841 cgttcctcct caaatgttac ttctcgaaac atgtgatcaa cgtgggattt cccccgcgtg
     3901 agaatggcgg cttttgccgc ctcactcaac accgctgatg cttcttcgta accgaaaggt
     3961 gccatggctt cctccccttc actaggacgc gttgatgcgt ccatgtagga ggtatagcgc
     4021 cgagtgggag ctccgtcaaa tacctactgg tactgaataa aagaaggaga cggggtaagt
     4081 tcgagcactt ccgcaggtgt gagaagacga gtggacggtg gcttcaagtc gttcttataa
     4141 aagagtttga acccggtgaa ctgcactggt tccggtttaa tgatggagtt ataggtgttt
     4201 attttctttg c
//
LOCUS       DAORIN010000051         4203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4431_length_4203_cov_4.870781, whole genome shotgun sequence.
ACCESSION   DAORIN010000051 DAORIN010000000
VERSION     DAORIN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4431_length_4203_cov_4.870781"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PK109_02525"
     CDS             complement(<1..517)
                     /locus_tag="PK109_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRH24437.1"
                     /translation="MKVSRTWLQKHFKEPLPDTAALAEALTFHAFEIEEYDEDMLDVK
                     VLPDRAAYALSHRGIAGELSAILNIPLSYDQFAELAPTFPTTDQLEVTGDAAYVIRHT
                     GALIRGVKVGPSPEWLKQSLESVGQRSINNVVDALNCVMLAMGQPSGAFDAGKLETDN
                     GVVRINIREARA"
     gene            complement(514..1242)
                     /gene="pheS"
                     /locus_tag="PK109_02530"
     CDS             complement(514..1242)
                     /gene="pheS"
                     /locus_tag="PK109_02530"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRH24438.1"
                     /translation="MSKGRLHPITVAIREIADIFGAMGFSLKYGPELEDDWHNFTALN
                     VPEHHPARDMQDTFWTKEQDPRVPRTHTTSVSIRCLEEMARTGQTSMRAIAPAKVFRN
                     EATDMTHEAQFYQLDGFAVGPDITLAHLKGTFDRFYREYFGPDAQVRFRPSFFPFTEP
                     SVEVDMWFQAPGKEGRWLEMAGAGMLHPNVLRNAGLDPDKVQGFAFGGGIERLLMVKY
                     GIPDVRLFHNGETRFVHGFEETTI"
     gene            1339..2589
                     /locus_tag="PK109_02535"
     CDS             1339..2589
                     /locus_tag="PK109_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRH24439.1"
                     /translation="MNLEILAYPFIFAAIYFEAFFLVTLLSAPAVASRKRTASTNTPM
                     VAMIVPCYNEEQTVSGTAESLLALDYPKDKLRIVLVDDGSTDGTASIMDSFAHHENVT
                     VIHQKNQGKHVALNAGIAAAEDAELIGCLDADSFVDASALREMIGGFDDGRVAASTAA
                     MSVHTPRTILERMQNAEYIMGIACRHILAAVNGIYVTPGPFSLYRRDVVVELGGFRNG
                     HNTEDMEMALRIQKAGYAIESAPLARVFTKTPRTVPALVKQRTRWTTGFLRNMLNEYR
                     DMVGNPQYGALGLLVLPLGFLAILGGIMMFFLVIYQLISQGIQMLMITSGVPVAYTLS
                     TMVPTFANASWFYLPVSIFALLGFVALAGSILFMVVGKNISKTPAQLSVGVIAYMLVY
                     GLIAPFWLLFSVADVATDNRRAWK"
     gene            2592..3266
                     /locus_tag="PK109_02540"
     CDS             2592..3266
                     /locus_tag="PK109_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24440.1"
                     /translation="MSLFTRNALIALLITVAIIGTVLYAVNFLNQQRVAELRSIEDQL
                     ATDTLSIETQFALLEEAPCEDIAAGTISQEVGGVGDKLAYAEERAGANDPEVLRLKER
                     YTLLQIRDYLLTKRLSETCGVNPVVALYFYSNVPGECEECDRAGYALSYLRQTYPALR
                     VYSFDYHLDLAALRTFISVLKVHDGEFPAFVIQGKPSYGFTSLEDLEKAFPKSLFATS
                     TTKASQ"
     gene            complement(3238..4065)
                     /locus_tag="PK109_02545"
     CDS             complement(3238..4065)
                     /locus_tag="PK109_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24441.1"
                     /translation="MRYAFYDSPAYKQKQSAITKRNQDLGLYEFNKKQEKRTCIRIGC
                     NVTFITARHNDKKYCSQSCSATVNNTQRALSDTARKKISNSLKGRTRAGIHNPLKGKI
                     IVPREERKCARLQCPKTFFIERWKKIRFCSVACAARRPTSPKASRGKAGIRPDISSSI
                     YFYSRWEANMARLYSYLGTVWEFSPRSFDIGGHTYTPDFFLPETDTYIEVKNFWGEYS
                     RIRDTKFRKRYPNILLKVILKDEYTRLEKKYAHLIPQWEYNHSPFSPHLIEKPLSSK"
     gene            complement(4123..4197)
                     /locus_tag="PK109_02550"
     tRNA            complement(4123..4197)
                     /locus_tag="PK109_02550"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4162..4164),aa:Thr,seq:tgt)
BASE COUNT          944 a         1111 c         1021 g         1127 t
ORIGIN      
        1 cggcacgtgc ttcacgaata ttaatgcgta cgacgccgtt atcggtttca agttttccgg
       61 catcaaaggc gcctgatggt tgtcccatcg cgagcatgac gcaattcaac gcatccacga
      121 cgttattaat cgagcgctgt cctacggact caagcgattg tttcaaccac tcaggtgatg
      181 gtccgacttt gacgccacga atgagggcgc cggtgtgacg aatcacgtat gcagcgtcgc
      241 ccgttacttc gagttgatca gtagttggga acgttggtgc tagctcagca aactgatcat
      301 atgagagcgg gatgttgagg attgccgaaa gttcgcccgc gataccgcga tgactaaggg
      361 cgtatgcagc gcgatcaggc agtaccttca catcaagcat atcctcgtcg tattcctcta
      421 tttcaaacgc gtggaaggtc aacgcttcgg caagtgctgc cgtatccgga agcggttctt
      481 tgaaatgttt ctggagccag gtgcgactta ctttcatata gtggtttctt cgaatccgtg
      541 cacgaagcgc gtctcgccat tatggaagag acgtacgtcc gggataccgt acttcaccat
      601 caagagacgt tcgataccgc caccaaatgc aaagccctgc accttatccg gatcaagccc
      661 tgcgtttcgt aatacgtttg gatggagcat gcccgcgcct gccatttcaa gccagcgtcc
      721 ttctttgccg ggcgcctgaa accacatatc cacttctacc gaaggctccg tgaacgggaa
      781 gaagcttgga cggaaacgta cctgcgcatc tggaccaaag tattcgcgat agaagcgatc
      841 aaaggttccc ttcaaatgcg caagcgtaat atcaggacct actgcaaacc catcgagctg
      901 gtagaactgt gcctcgtgcg tcatgtcggt tgcttcgttg cggaatactt ttgcaggagc
      961 gatggcacgc atggaggttt gcccggtacg tgccatttct tcaaggcagc gaatggaaac
     1021 tgaggtggtg tgggttcttg ggacgcgcgg atcctgctcc ttggtccaga aggtgtcctg
     1081 catatcgcgg gcgggatggt gctccgggac gttcagcgcc gtgaagttat gccagtcatc
     1141 ctcaagttca gggccatatt taagggagaa acccatagca ccgaaaatgt ccgcaatctc
     1201 acggatagcg acagttatgg ggtggagacg acctttagac atatcttgtg cagtatagcg
     1261 acgtttcgtg gaaaaaacag gccaaatttg ctatagtaat ggagtaattg agcggcgccc
     1321 acgctccgcc caggcaccat gaatctagag atactcgcct atccgttcat ctttgcggcc
     1381 atctactttg aggccttttt cctcgtaacc ctcctttctg ctccagcggt agcaagccgt
     1441 aagcgcaccg catcaaccaa tacgccgatg gttgccatga ttgtcccttg ctacaacgaa
     1501 gagcagacgg tgagtggtac cgcagagtcg ttgcttgcgc tcgactaccc gaaagacaaa
     1561 ctccgcatcg tgctcgttga tgacgggtct acggacggca ccgcttccat catggattct
     1621 tttgcgcatc acgaaaacgt taccgtcatc catcagaaga accagggcaa gcatgtagcg
     1681 ctcaacgccg gtatcgccgc tgctgaagac gcagaactca tcggctgcct tgacgccgac
     1741 tcattcgtag acgcgagcgc gcttcgtgaa atgatcggtg gttttgatga tggacgcgtc
     1801 gccgcctcta ccgcagccat gtccgttcat accccgcgca ccatccttga gcgcatgcag
     1861 aacgctgagt acatcatggg tatcgcctgc cgtcatattc ttgctgcagt gaatggcatc
     1921 tatgtgacgc caggaccatt ctccctctac cgccgcgatg tcgttgtgga gcttggcgga
     1981 ttccgcaatg gccataacac cgaagacatg gagatggcac ttcgtattca gaaagcaggc
     2041 tacgccattg aaagcgcgcc acttgctcgt gtgttcacca agacgccgcg taccgttccg
     2101 gcactcgtga agcagcgcac acgctggacc actgggttcc tgcgcaacat gctgaacgaa
     2161 taccgcgata tggtaggtaa cccgcagtac ggtgcgcttg gccttctcgt gcttccgctt
     2221 gggttcctcg ctatcttggg cggcatcatg atgttcttcc tcgttatcta ccagctcata
     2281 agccagggca tacagatgct tatgattacg tcaggcgttc cggtggccta caccctttca
     2341 actatggtcc ctacctttgc aaacgcgagc tggttctacc tccccgtttc tatatttgcc
     2401 cttcttgggt ttgtcgcact cgcaggctct atccttttca tggttgtagg aaagaacatt
     2461 tctaagactc cggctcagct ctcggttggg gttatcgcct acatgcttgt gtacggactc
     2521 attgctcctt tctggcttct gttctcggta gctgacgttg caactgataa ccgccgcgcc
     2581 tggaaataat catgtctctc ttcacccgta acgctcttat cgcgctcctt attacggtag
     2641 cgattattgg taccgttctc tatgcggtta acttcttgaa ccaacagcgc gttgcggagc
     2701 tccgctctat tgaggaccag ctcgccaccg acacgctttc catagaaacg cagttcgcgc
     2761 ttcttgagga agcaccgtgc gaagacattg cagcaggcac catttcccag gaagtcggtg
     2821 gcgtgggaga taagctcgca tacgctgagg agcgcgccgg tgccaacgac ccagaagtgc
     2881 ttcgccttaa ggagcgctac acccttctcc agattcgtga ctatcttctc acgaagcgtc
     2941 tttcagagac ctgtggtgtg aatcctgtcg ttgctctcta cttctattca aacgttccgg
     3001 gagagtgtga ggagtgcgat cgcgcaggct acgccctctc ctaccttcgc cagacgtacc
     3061 ctgcactccg cgtctattcc ttcgactacc atctcgacct tgcggcactg cgtaccttca
     3121 tttccgtact taaagtccat gacggtgagt tccctgcctt tgttattcaa ggcaaacctt
     3181 cctatggatt cacctcacta gaagatcttg agaaggcatt ccctaagtca ctctttgcta
     3241 cttcgacgac aaaggcttct caataaggtg tgggctaaat gggctgtggt tatattccca
     3301 ctgagggatt aggtgggcgt atttcttttc aagacgtgtg tattcatcct ttaaaataac
     3361 ttttaataaa atattcggat atcgttttct aaactttgtg tcacgtattc tcgaatactc
     3421 accccagaag ttcttaactt caatataggt atctgtttct ggaagaaaga agtcaggagt
     3481 ataggtatgt cctccaatat caaaggatct tggtgaaaac tcccatacgg tgccgagata
     3541 cgaatagaga cgcgccatgt tcgcctccca tcgactgtag aaatatatag aggaacttat
     3601 atccggacgt atgcccgctt tgcctcttga ggcttttgga gaagttggac gtcgagcggc
     3661 acatgccaca ctacaaaaac gaatcttttt ccatctctct atgaagaaag tctttgggca
     3721 ctggagacgt gcacatttcc tttcttccct tggaactata attttacctt tgagagggtt
     3781 atgtataccg gcgcgtgtac gaccttttaa agaattactt attttcttcc ttgccgtatc
     3841 cgatagtgct ctctgagtat tgtttactgt tgcagagcaa ctttgactac aatacttctt
     3901 atcgttatgg cgtgctgtaa tgaaagttac attacaccca atgcggatgc aggttctttt
     3961 ttcttgcttt ttattaaatt cgtataaacc tagatcttga ttacgctttg taatcgcgct
     4021 ttgtttttgt ttatacgcag gagaatcata aaatgcgtat cgcatctata gacgatatca
     4081 atccctatgg tggtgggttt cccctattac tacacaactt cttggagcca ctttccgaac
     4141 ttgcatcgga ttctgcgggt tacaaaaccg ctgctctact atatgagcta aagtggcgtg
     4201 gtt
//
LOCUS       DAORIN010000052         4202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4433_length_4202_cov_6.483723, whole genome shotgun sequence.
ACCESSION   DAORIN010000052 DAORIN010000000
VERSION     DAORIN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4433_length_4202_cov_6.483723"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK109_02555"
     CDS             complement(<1..205)
                     /locus_tag="PK109_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRH24442.1"
                     /translation="MANTKLYVGGIPYRTTEDELRTAFEEAGTVTSASIIMDRMTGRS
                     RGFGFVEMADEASAQAAIDRWDGK"
     gene            429..707
                     /locus_tag="PK109_02560"
     CDS             429..707
                     /locus_tag="PK109_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24443.1"
                     /translation="MTLFSTDLIVNFTIVLLALMGLAGLMISLQRRKPVTQAFGRAMF
                     FGALAVICLLEYGYTTKLWIIGIVLFVVPQLYWSYQAVKLWYQRWGTA"
     gene            complement(753..1391)
                     /locus_tag="PK109_02565"
     CDS             complement(753..1391)
                     /locus_tag="PK109_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRH24444.1"
                     /translation="MTPKLLVFATGTPEGGGSGFENLVNASRDGRLTAEVVGVVCNFE
                     SGGVREKADRLGIPFIYMPGPYTAEAYLKIVSDTDADFAALSGWLKLVKGLDPKTTFN
                     IHPGPLPEFGGAGLYGHFVHEAVMKAYQEGRITHSAVSMHFVTPEFDEGPVFFSHQVE
                     IFPDDTADTLGKRVNEAEHRYQPEITNLVLNGTISWDGKEPDTLRGARRESI"
     gene            complement(1388..3052)
                     /locus_tag="PK109_02570"
     CDS             complement(1388..3052)
                     /locus_tag="PK109_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRH24445.1"
                     /translation="MALLDELKKNIKGGVSNSSAERAKFSHDTSLFERIPSVVVYPKN
                     AADVSTLVKAVRGAKEAGEDVSVTARSAGTDMSGGPLTRSVVAVFTKYMNKVVEVADG
                     YAVTEPGAYYRDFEKATLAKGQILPSYPASRSICAMGGIVNNNSGGERTLEYGKTEKY
                     VEEVDVVLSDGTQTTFKALGPDELETKKKQTDLEGEIYRRMSELVIKNRELIESHRPK
                     VSKNSAGYALWNILDIENGTFNLAKLICGAQGTLALMTKAKLSLVKPKEHRAMLVVYL
                     PSDHGHTKLEVLPEIVRRVLPHNPESFESYDDHTLSLAVRFLPQMLMQMGLLRAIRLG
                     ISFIPEVVTAITGGIPKLILMAEFAEDTMEEADKRAEAAAAALKDLKLPMFIERDERA
                     SEKFWIVRRESFALLRKNLSGLYAAPFIDDFVVPPDSYPKFLPELNALLAKYDLVYTI
                     AGHIGNGNFHIIPLMDLAKPETKKIVMELGPLVYDLVIKYGGTTTGEHNDGIIRTPYV
                     EQLFGSEMTRLFRETKHIFDPLNIFNPGKKVGGTFADIEDSFLKKA"
     gene            complement(3060..3815)
                     /locus_tag="PK109_02575"
     CDS             complement(3060..3815)
                     /locus_tag="PK109_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRH24446.1"
                     /translation="MSLPKVVAFDLDGTLAVSKSQVSPEMGTLLAELTKKLPVAIMSG
                     GKYAQFQKQVLTALPEDAHLENLYLFPVCAGYCYHFVDGEWKLVDDNSYTPEEKAVIM
                     AALEESMAETGFTEPPTPVWGERIEDRGALIAFSGLGQEAPWEAKKEWDPHKEKRRPL
                     WAALVARLPDCEVKMNAATTIDITRKGITKAYGVRKLSEFTGVPISEMLYVGDALFPG
                     GNDEVVIPTGIPTRPIADTNETAAVIKEILAQV"
     gene            complement(3818..>4202)
                     /locus_tag="PK109_02580"
     CDS             complement(3818..>4202)
                     /locus_tag="PK109_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRH24447.1"
                     /translation="SGTRISRCIEIMGPREGIWDAAKVGMAGPLLKVDGGWLMLYHGV
                     SRRGRYRVGAALLDEAGTTVLSRTADPIFEPLEKYEIEGEVGHVVFPCGAVIRDDTLY
                     MYYGGGDKVVGVATGSLSRILSALR"
BASE COUNT          967 a         1098 c         1099 g         1038 t
ORIGIN      
        1 ccttgccatc ccaacggtca atagcagcct gggccgaagc ctcatctgcc atttcgacga
       61 agccgaatcc gcgtgagcgg ccggtcatac ggtccatgat aatgcttgca gacgtgaccg
      121 tacctgcctc ttcaaaggcg gtgcgaagct cgtcttccgt cgtgcgataa ggaataccgc
      181 cgacgtatag tttggtgtta gccatgtgtg aacttgattt gtaacctcgc cgataccgaa
      241 caccttggat ttgaggccgt agctcctgct acctgcgagt tatcagcact atgctacgga
      301 tagcctttaa aatcaacctt ttttcgagtg ggtgtggatt atgtaaggaa ttatttgact
      361 aatatatttt tgtgtcgtaa tgtaaataac gaggccccgt ataacatggg tgcataagga
      421 gcggtcgcat gaccctgttc tcaaccgatc tgatagtcaa cttcactatt gttctgctcg
      481 cgctcatggg cctcgccggc ctgatgatta gtctgcaacg gcgtaagccg gtgacgcagg
      541 cattcggtcg cgccatgttt ttcggtgcgc tcgcggtcat ttgtcttctc gagtacgggt
      601 acacaacgaa gctctggatt atcggaattg tcctattcgt cgtgccacaa ctctattggt
      661 cgtaccaggc tgtgaagctt tggtatcaac gatggggaac ggcgtgaatt gaagaaaggt
      721 gcgcgcttct gaaagggagc gcgcatcact ttttatatag attctctacg agcaccacga
      781 agcgtgtctg gttctttccc atcccatgaa atggttccgt tcaggacaag gttcgttatt
      841 tccggctggt agcgatgctc agcttcattc actcgtttcc caagagtatc ggcagtgtcg
      901 tccggaaata tctctacctg atggctgaag aatactgggc cttcatcaaa ctcgggcgtc
      961 acaaagtgca tagataccgc cgagtgagtg atccgtcctt cctggtatgc cttcatcact
     1021 gcttcgtgca caaagtggcc atagagaccg gcgccaccaa attccgggag aggtccggga
     1081 tgtatgttaa aggtcgtttt tggatcaagt ccctttacga gcttcagcca cccagaaagg
     1141 gcagcaaagt cagcgtcggt gtcgctcact attttcaaat atgcttcggc cgtatagggt
     1201 cccggcatat agataaaggg aataccaagt cgatccgctt tctcacgtac accaccactt
     1261 tcaaagttac atacgacgcc aacaacttca gcagtgaggc gtccatcacg tgacgcattc
     1321 acgaggtttt caaatcctga cccgcctcct tccggagtac cggtagcaaa gacgagaagc
     1381 ttcggggtca tgctttcttc aagaaactat cttcaatgtc agcgaaggtg ccgcccactt
     1441 tctttcctgg attgaagata ttgagggggt cgaagatatg cttcgtctct cggaagaggc
     1501 gtgtcatctc acttccgaac aattgctcta cgtacggcgt gcggatgatg ccgtcgttat
     1561 gttctcccgt agtggtgccg ccatatttaa taacgaggtc atacaccaat ggcccaagct
     1621 ccatcacgat cttcttagtc tcgggcttcg cgagatccat aagcggaatg atatggaagt
     1681 taccgtttcc gatgtgtccg gcaatcgtgt acacaagatc gtactttgca agaagtgcgt
     1741 tcaactcagg aaggaacttc gggtaggaat cagggggaac gacaaagtca tcgatgaacg
     1801 gagcagcata aaggccagag agattctttc gaagaagtgc gaaggattca cggcggacaa
     1861 tccagaactt ctccgacgca cgctcatcgc gctcgatgaa catagggagt ttcaaatcct
     1921 taagcgctgc tgccgctgcc tctgcacgct tgtccgcctc ttccatggtg tcttcggcga
     1981 actctgccat gaggatgagc tttggaatac cgcccgttat tgccgttact acttctggga
     2041 taaaggagat gccaaggcgt atggcgcgca ggagccccat ctgcataagc atctgaggga
     2101 ggaagcgtac ggcaagcgaa agcgtgtgat cgtcgtatga ctcaaaggac tcagggttgt
     2161 gcggaagaac acgacggaca atttctggga ggacctcaag cttagtgtgg ccatggtccg
     2221 aagggagata tacgaccagc atggcgcgat gttctttcgg cttcacgagc gagagctttg
     2281 ctttggtcat aagggcaagg gttccctgag cgccacagat gagtttcgcg aggttaaagg
     2341 taccgttctc aatatcgaga atattccaga gtgcgtagcc ggcggagttc ttagagactt
     2401 tcggacgatg cgactcgatg agttcgcggt tcttaatgac aagctcgctc atgcggcggt
     2461 atatttcacc ctcaagatca gtctgcttct tcttcgtctc aagctcatca ggaccaagcg
     2521 ctttgaacgt ggtctgggtc ccgtcagaga ggacaacgtc gacttcttcc acatacttct
     2581 ccgtctttcc atactcaagc gtgcgttcac ctcccgagtt gttgttcacg atgccgccca
     2641 tggcgcatat agaacgggaa gcagggtagg agggaaggat ctggcctttc gctaaggtcg
     2701 ccttctcaaa gtcacggtaa taggcgccgg gttccgttac ggcatatccg tccgcaacct
     2761 cgactacctt attcatatat ttggtgaaca cagcgaccac tgagcgggtc agcggaccac
     2821 ccgacatatc agttcctgcg gagcgcgccg ttacggaaac atcctcgcct gcttctttcg
     2881 ctccacgtac tgctttaacg agcgtcgaaa cgtccgcagc attctttggg tatacgacga
     2941 cggaaggaat gcgctcaaag agtgaggtgt catgagagaa cttagcgcgc tccgcagagg
     3001 aattagaaac ccctcctttg atattcttct ttagttcgtc gagtaatgcc atgacgaaat
     3061 tatacctggg caaggatttc cttaatgact gccgcagtct cattggtatc cgctattggg
     3121 cgcgttggga taccggtagg gataactact tcatcgttgc ctcccgggaa gagagcatca
     3181 ccgacataca acatttcaga aataggtacg ccagtgaatt cacttagctt ccgtacccca
     3241 tacgcttttg ttatcccttt gcgggtgatg tcgatagtgg tcgctgcatt catcttcact
     3301 tcgcagtcgg gaaggcgtgc aacgagcgcg gcccacaatg gacgacgctt ctctttatgt
     3361 gggtcccatt ctttttttgc ttcccacggc gcctcctgac caagcccgga gaaggcgata
     3421 agagcaccgc ggtcttcaat gcgttcaccc cacacgggag taggaggctc agtgaatccg
     3481 gtttctgcca ttgattcctc aagggctgcc atgatgactg ctttctcttc gggagtgtag
     3541 gaattgtcat caactaactt ccactcacca tcaacaaagt ggtagcagta gccggcacag
     3601 accgggaaga gatagaggtt ttcaaggtgt gcatcctcag gaagcgcggt gagtacctgc
     3661 ttctggaact gtgcgtattt gcctcctgac atgattgcaa ccggaagttt cttggtgagc
     3721 tctgccagta acgttcccat ttctggtgag acctgagact tactcacggc gagcgtgccg
     3781 tcgagatcaa aggcaactac ttttggaagt gacatagtta gcgtagtgcg gacagtatac
     3841 gtgaaagaga cccggtggcg acgcccacca ctttgtcgcc cccgccgtag tacatataaa
     3901 gggtatcgtc acggataacg gcgccgcagg ggaatacgac gtgccctacc tcgccctcaa
     3961 tctcatactt ctcaagaggc tcaaatatcg ggtctgcggt gcgcgaaagc acggtagtcc
     4021 ccgcctcatc aagaagggcc gcacccacgc gatagcgacc acgacgcgat acgccgtgat
     4081 agagcataag ccagccaccg tctactttaa ggagggggcc cgccatacct actttggcgg
     4141 cgtcccatat accttcacgt ggtcccatga tttcaatgca gcgggatatg cgcgtgccgc
     4201 tc
//
LOCUS       DAORIN010000053         4148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4526_length_4148_cov_4.568532, whole genome shotgun sequence.
ACCESSION   DAORIN010000053 DAORIN010000000
VERSION     DAORIN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_4148_cov_4.568532"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PK109_02585"
     CDS             <1..632
                     /locus_tag="PK109_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRH24448.1"
                     /translation="DSAFRKILIALAVYITSLLAANTLGIKLMPFLFGSHLSVGVFMF
                     PVVFLMTDVIGEVYGKKVAKYFVFAGIISTLLFIAYNVISVVAPWSADGLWAKESYEQ
                     IFGVSLRFAIASVIAYAIGEYQDVLSFFFFRKKLGEKSGFWLRSFLSNVWSQFFDTVL
                     FMVIALYGVYPNDVLISAILTWWAFKVAMGLLYTPLAYVGIRLLKEKAA"
     gene            complement(681..935)
                     /locus_tag="PK109_02590"
     CDS             complement(681..935)
                     /locus_tag="PK109_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24449.1"
                     /translation="MATIINTPPTNDTGNSATIWVLGLIIIVLIGLFAFFVWPGMDNQ
                     AAAPTPIPTEERAATPQQPNVYNTTINATSTTVNGTTTLP"
     gene            1036..1755
                     /locus_tag="PK109_02595"
     CDS             1036..1755
                     /locus_tag="PK109_02595"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HRH24450.1"
                     /translation="MQVQPVKTRIFKEGESLADFVVAHIPTLAEGSVLVVTSKIVALA
                     EGRTSDATDEKTKERLIRAESTFALKTKWVWLTLRDRLVIANAGIDESNSAGKLVLLP
                     KDSYASAKALRTTLMKRYGIRRLGVILTDSRILPLRTGVMGVTLGYAGVKGLRDYIGQ
                     KDIFGRKFQFEKTNVVDCLASAAVLCMGEGAERQPLAVITGAPVAFATTVRKDELAID
                     LKDDIYLPLFAKIPKALLKKR"
     gene            1805..2371
                     /gene="infC"
                     /locus_tag="PK109_02600"
     CDS             1805..2371
                     /gene="infC"
                     /locus_tag="PK109_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRH24451.1"
                     /translation="MSTDRTRINGEIRAEKLRVIGAEGENLGVLSFDEAMKAAEAAGL
                     DLIEISPNAVPPVAKVMDYGKFQYELKKKESAARAKAKVSETKEVQIKVGTGENDMRL
                     KAKKAAEWLAEGHRVRAELFLKGRYKGMDEAFLKVRLEKFLLLIPYAYRVSEPVAKSP
                     KGFAAIIERDPGAAKKQPSDTKETHENK"
     gene            2358..2576
                     /locus_tag="PK109_02605"
     CDS             2358..2576
                     /locus_tag="PK109_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRH24452.1"
                     /translation="MKTNKSFTKRIRVSRTGKLLARKPGQDHFNAKERGRTKGDKKRG
                     VQLAMSNRLMRRFLGGNGTKRAKKVVAE"
     gene            2596..2943
                     /gene="rplT"
                     /locus_tag="PK109_02610"
     CDS             2596..2943
                     /gene="rplT"
                     /locus_tag="PK109_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRH24453.1"
                     /translation="MPRVKGGVQSAKTRRNVLKMAKGYRHGRSKKERAAYESLVHAGK
                     QAFAHRRDKKNDFRQLWIVRLNAAIRENGYKNYSTFIDSLKKANVLLDRKVLSEMAVN
                     HPEVFTRIAKKVG"
     gene            complement(2989..3168)
                     /locus_tag="PK109_02615"
     CDS             complement(2989..3168)
                     /locus_tag="PK109_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24454.1"
                     /translation="MAPEGISWTGEELGPNDREINPTPWRKHWKPKSSGGGELPQKNI
                     DHPDELPPGEYSDYN"
     gene            complement(3173..>4148)
                     /locus_tag="PK109_02620"
     CDS             complement(3173..>4148)
                     /locus_tag="PK109_02620"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HRH24455.1"
                     /translation="EEREVAPGLSVMLRDTGHILGSASVRVRAADGTAFAVTSDIGNS
                     PALLLPDAEPIPDADVILIESVYGDRANPEKAHRVEKLQQVLTRALARGGTVLIPSFS
                     IERTQLMLYEIGMLFAANKVPEVPVFLDSPLAITVTEIYQKWGGEYFNTDAKEEMGHG
                     HNLFEFPFLTMTRSHEDSDAIREKHGPKIIIAGAGMSHGGRIGRWEQQYLGDPKTTLI
                     MVGYQAPGSPGRMMQDGAKSVRIDRETVKVAATIEVLHGWSGHADRDGLLAFAEKCIP
                     RAKSFIVGLGEPSSARFLAQRIHDYLGVKAIVPTEGEVFNITKNGVVR"
BASE COUNT          936 a         1121 c         1100 g          991 t
ORIGIN      
        1 ctgattcggc cttccgcaag attctcatcg cccttgcggt ctatatcact tctctgcttg
       61 ctgccaacac gctcggcatt aagttgatgc ccttcctgtt cggctcacac ctctcggtgg
      121 gcgtattcat gttcccggtg gtgttcctca tgaccgacgt catcggcgaa gtgtacggaa
      181 agaaagttgc caagtacttc gtgttcgcgg gtattatttc aacgcttctc tttatcgcgt
      241 ataacgtgat ttcagttgtc gctccgtggt cggcagacgg cctctgggca aaggagtcgt
      301 atgagcagat attcggtgtg tcgctccgct ttgctatcgc atccgtcatc gcgtacgcca
      361 ttggtgaata ccaggacgtg ctttctttct tcttcttccg caagaagctt ggggaaaaga
      421 gcggattctg gctccgttcc ttcctctcaa acgtgtggag tcagttcttc gatactgtgc
      481 tctttatggt catcgcactc tatggcgtgt acccgaatga tgtcctcatc tccgccatac
      541 ttacctggtg ggcattcaag gtagcgatgg gtcttctcta caccccactg gcgtacgtcg
      601 gtattaggct gctcaaggag aaagcagcgt aaggtaaaag aaaaacagcc gcccactcgg
      661 gcggctgttt ttctttttag ctacggaagg gttgtcgttc cgttcaccgt tgtactcgtc
      721 gcgttgatgg tcgtgttgta cacgtttggc tgctgcggcg ttgcggcacg ctcttcagta
      781 ggaataggcg taggtgcggc agcctggtta tccatgccgg gccagacgaa aaaggcaaag
      841 agtccgatga ggacgataat gatgagtccg agtacccaga ttgttgccga gttaccggtg
      901 tcgttcgtgg gcggcgtatt gatgatggta gccataggtt ctagtgaatt ggttagacct
      961 tcataatagg cagagtgtgg ttaagggttc atgaaggtgg agttttaccg gtgaggctcg
     1021 tatactgccc ccacgatgca ggtacagccc gtaaagacac gaatcttcaa ggagggagaa
     1081 tctcttgctg attttgtggt ggcacatata ccaacattgg ctgaaggatc tgtccttgtg
     1141 gttacctcaa aaattgtggc gcttgccgaa ggacgcacgt cagacgctac tgacgagaaa
     1201 acgaaggaac gcctcatacg agccgagagt acgttcgctc tcaaaacaaa gtgggtctgg
     1261 ctcacactcc gtgatcggct cgtcattgca aacgccggta tcgatgagtc caatagtgct
     1321 gggaaactgg ttctccttcc taaagactct tacgcaagtg caaaagcgct gcgtaccacc
     1381 cttatgaaac ggtacggtat acgtcgtctt ggcgtcatcc ttactgatag tcgtatcttg
     1441 ccgcttcgta cgggcgtgat gggagtgacg cttggctatg cgggcgtgaa aggattacgc
     1501 gactacatcg gtcagaaaga catctttgga agaaagtttc agttcgagaa gacgaacgtc
     1561 gtcgactgcc tcgcgtcagc tgctgtcctc tgtatgggag aaggggctga acgccaaccg
     1621 cttgcagtta ttactggagc gcccgtagcg ttcgctacga cagtccgaaa ggacgaactt
     1681 gccatagacc tcaaggacga catctatctc cccctcttcg ccaagattcc aaaggctctc
     1741 cttaagaagc gctaaaggcc tggatatacg ctctttatag tgctatagtc cctctaacac
     1801 cgccttgagt accgacagaa cacgcattaa tggagagata cgggctgaaa agcttcgcgt
     1861 cataggcgcc gagggagaga acctcggcgt attatccttt gatgaggcca tgaaagcagc
     1921 ggaagccgca ggcctcgacc tcatagagat ctcgccgaac gccgtcccgc ccgttgcgaa
     1981 agtcatggat tatggtaaat tccaatacga gctgaagaag aaggagagtg cggcccgggc
     2041 caaggctaaa gtgtctgaaa ccaaggaggt ccagatcaag gtcggcacgg gggaaaacga
     2101 catgcgtctc aaggctaaga aggcagccga atggcttgcc gaagggcatc gcgttcgcgc
     2161 ggagctcttc ctcaaaggcc gctataaagg gatggatgaa gcgttcctca aggtccgcct
     2221 cgagaaattc ctgctcctca ttccgtacgc ctaccgtgtt tccgagccgg tagcgaagtc
     2281 ccctaagggc tttgctgcca tcatcgaacg agacccgggt gctgcgaaaa aacagccttc
     2341 cgacaccaaa gaaacccatg aaaacaaata agtcatttac gaagcgcatc cgtgtatctc
     2401 gcacgggcaa actccttgcc cgcaagccag gtcaggatca tttcaacgca aaggagcgtg
     2461 gtcgcaccaa gggcgacaag aagcgtggcg tccagctcgc tatgagcaac cgcttgatgc
     2521 gccgtttcct tggaggcaac ggtacgaagc gcgcgaagaa agtcgtcgct gaataatccc
     2581 gttaattaat tttctatgcc acgagtaaaa ggaggagttc agtcagctaa gacccgccgc
     2641 aatgtattga agatggcgaa gggctatcgc catggccgct cgaagaagga gcgcgctgcc
     2701 tacgagtccc tcgttcacgc tggtaagcag gcttttgctc accgccgcga caaaaagaac
     2761 gacttccgcc agctctggat cgtccgcctc aatgcggcta tccgcgagaa cgggtacaag
     2821 aactacagca ccttcatcga ctcattgaag aaggccaatg ttctccttga tcgcaaggta
     2881 ctctcggaga tggcagtgaa tcacccagag gtgttcaccc gcatcgcgaa gaaagttggg
     2941 taatcccctc cccttaagaa aagccccgct cgagcggggc ttttcttttt agttatagtc
     3001 agagtactca cctggtggta attcgtctgg atggtctata ttcttttgcg gaagttcccc
     3061 tcccccggaa gatttaggtt tccaatgttt acgccatggc gtcgggttta tttcccgatc
     3121 atttggtcca agctcttctc cagtccaaga tattccttct ggtgccatat ccctagcgta
     3181 ccactccgtt cttcgtaatg ttaaacactt ccccttcagt gggcactatc gcttttacgc
     3241 cgagatagtc atggatgcgc tgcgccagaa accgtgcaga ggacggttca ccaagtccca
     3301 cgataaacga ctttgcgcgt ggaatacatt tctctgcaaa tgcgaggagg ccatcacggt
     3361 ccgcgtgccc actccaacca tggaggactt ctatagttgc tgcgactttt acggtttctc
     3421 tatcgatgcg cacggacttt gcgccgtcct gcatcatgcg tcccggcgac cccggtgcct
     3481 ggtaccccac catgatgagg gtggtctttg ggtctcctaa gtactgttgc tcccaccgtc
     3541 cgatgcgtcc gccatgactc ataccagcgc ccgcaataat gatctttggt ccatgctttt
     3601 ctcgtatcgc gtctgagtcc tcatgcgagc gcgtcatcgt aaggaatggg aactcaaaaa
     3661 ggttatggcc gtgacccatc tcttcttttg cgtctgtatt gaaatactca cctccccact
     3721 tctgatagat ctccgtcacc gttatggcaa gcggcgagtc gagaaacacc ggcacctctg
     3781 gtaccttatt tgccgcaaac aacatcccta tctcatagag catgagctgc gtccgctcga
     3841 tggagaacga cgggataagg acggtaccac cacgcgcaag cgcacgagtg agtacctgct
     3901 gcagcttctc aacgcgatgc gccttctcag ggttagcccg atctccgtac acgctctcaa
     3961 tgaggatgac gtcggcgtca ggaataggct cagcgtcagg gagaaggagc gctggtgagt
     4021 tcccgatatc gctcgtgacg gcaaatgctg tgccgtcagc agcacgcacc cgtacgctcg
     4081 ctgacccaag tatgtggccc gtgtcccgga gcatcaccga gagtccaggc gctacttcgc
     4141 gctcttct
//
LOCUS       DAORIN010000054         4133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4550_length_4133_cov_5.334478, whole genome shotgun sequence.
ACCESSION   DAORIN010000054 DAORIN010000000
VERSION     DAORIN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4550_length_4133_cov_5.334478"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PK109_02625"
     CDS             <1..940
                     /locus_tag="PK109_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRH24456.1"
                     /translation="YGFNKAHAASYGKVAYQTAYMKANYPVEYLAALLTADAGDVEKI
                     AILVAEADRLKIPVLPPDVNESRATFTVVGENKDAVRFGLSSIKNFGEGIAEAIIAER
                     EANGPFATLSDFLARIGSKNLNRKSLESLIKCGALDALGDRGHLLQHIETLLTFHRDA
                     TAPVSQDTLFAATTPTLILPPGTATVSLTDKLDWERELLGIYVSGHPLDAHTDKTMKA
                     HVTIGSVVADPHPGLPLIIPVLISDVRTILTKNGEKMAFVKLEDKSGTIEAVVFPKLF
                     KEHGAALVAGRCMLVKGTVSSRNGELSIAVDNLKPL"
     gene            997..1149
                     /locus_tag="PK109_02630"
     CDS             997..1149
                     /locus_tag="PK109_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24457.1"
                     /translation="MRRRQKMREDLEREFPHMSDDVISRTVKRIEEIIDENVDEAFKE
                     VEGGDP"
     gene            1211..1534
                     /locus_tag="PK109_02635"
     CDS             1211..1534
                     /locus_tag="PK109_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24458.1"
                     /translation="MTIPLPPVAAAHMALRAKGREREPLTRLAEWVETHSKTYGSLFL
                     SDTYGLPLNQTGESCSTNDEKIATLIRLLREAAGEHGVFNQSERDYLGRVLRVWSEHQ
                     AVRLS"
     gene            1681..1995
                     /locus_tag="PK109_02640"
     CDS             1681..1995
                     /locus_tag="PK109_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24459.1"
                     /translation="MIEDAERQVRALRETNLCEPEHIQPLIEWLRDREENEGLIRSSR
                     LAEAFQTVNPTVCSRSLVYPTRTLRETLEALSAGKDKSQDVFDFTLNVLSAWSDIQCA
                     HA"
     gene            2064..3806
                     /gene="thrS"
                     /locus_tag="PK109_02645"
     CDS             2064..3806
                     /gene="thrS"
                     /locus_tag="PK109_02645"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRH24460.1"
                     /translation="MTSLEHKRHTLAHLLAAAVLETYPDAKRTIGPAIDTGFYFDFEF
                     PTEKPTEESLKKLEAKMRKLLPSWKSMERSELSAEAAKAEYPGNEYKHELIDEFSGEG
                     QTLTFYTSGHYSDLCRGGHCENPAKEIAGDSFMLDRIAGAYWRGDEKNPMLTRIYGLA
                     FDTKEELDAYLANREEAKKRDHRKIGREQGLFVFSDLVGSGMPLFTPKGNIIRSQIIG
                     FSRELNEALGFGEVHTPNVNKGELFKISGHYDQYKEDMLSVRSQYVEDEMFLKPMNCP
                     QHTQIYASAPRSYRDLPVRFSDFATLYRDERPGELSGLTRLRAFAQDDGHIFCREDQI
                     EEEISAVLAAIQKALAVYRIDYWMRLSLRDPQNKEKYLGADEGWVKAEAIMRKLLTEK
                     NITHKEAEGEAAFYGPKIDIMAVDALGREWQISTIQLDFVQPTRFQLEYTSDDGSKKT
                     PVMIHRALVGSPDRFLGILIEHYAGAFPAWLAPIQARILPVSDKHAAYGAEILAQLKG
                     AGIRAELDDANESLGKKIRNGKTDKVPYLLVIGDAEVDAKTVSVESRDHGKLDAMNIA
                     AFIERVQNEIATRA"
     gene            complement(3803..4075)
                     /locus_tag="PK109_02650"
     CDS             complement(3803..4075)
                     /locus_tag="PK109_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24461.1"
                     /translation="MSVDAILYFLVLALWSGVISYLLLFKILPRITEKEVATPVEHHA
                     PAPKAHHAPAQHTPRPRYSSHEGFRSFAKGEELTVEDIVKGLSGGR"
BASE COUNT         1025 a         1108 c         1086 g          914 t
ORIGIN      
        1 ctacggcttt aacaaagcgc acgccgcaag ctacggcaaa gtcgcgtacc agacggcata
       61 catgaaagcg aactaccctg ttgagtacct tgccgccctg cttacggcag acgctggcga
      121 cgtcgagaag attgccatac tcgtggcgga agcggaccgc ctcaagattc ccgtactccc
      181 gcctgatgtg aacgaaagtc gcgctacctt taccgtcgtt ggtgagaaca aggacgccgt
      241 acgtttcggt ctctcttcta ttaaaaactt cggtgaaggt atcgcagaag ccatcattgc
      301 ggagcgggaa gccaacggtc cctttgctac cctttctgat tttctggcgc gcatcggatc
      361 aaagaacctg aatcgtaaat cgcttgagtc cttgattaag tgcggtgcac ttgatgcgct
      421 tggagaccgc gggcacttgc tccagcatat tgaaaccctg ctcaccttcc accgcgacgc
      481 cactgcgccc gtgtctcaag acacactctt tgcggcaacc actccaacgc ttatcttgcc
      541 tcccggcacc gctaccgttt cattgaccga caagcttgat tgggagcgtg agctgcttgg
      601 catttatgtc tccggccacc cgcttgacgc acacaccgat aaaacgatga aggcgcatgt
      661 gactataggg agcgtcgtcg cggacccgca tccgggactc ccacttatca ttcctgtcct
      721 catttcggac gtgcgcacca tccttaccaa gaacggtgag aagatggcct tcgttaaact
      781 tgaggataag tcgggcacta ttgaagcggt ggtgttcccg aaactgttta aggagcatgg
      841 cgcggcactc gtggcgggcc gctgtatgtt agtaaaagga acggtctcca gccgcaacgg
      901 cgagctctca attgcggtcg ataacctgaa gcccctttga tatagtcttc gtgaaggagg
      961 tacgcgatga gcaaaccacc ggatactgcc gaatctatga ggagacggca gaagatgcgt
     1021 gaagatcttg aaagagaatt cccgcacatg agcgatgatg ttatttctcg aacagtaaaa
     1081 cggattgaag agataatcga cgaaaatgtc gatgaagcct tcaaggaagt agaaggcggc
     1141 gacccataat cgccgccttt ttgttttttt gataaggtga gactgggctg ggaggagaag
     1201 gatttgcact atgactattc cacttccccc ggtcgctgca gcgcatatgg cgctccgagc
     1261 aaaaggccgt gaacgtgagc ctcttacccg gctcgctgag tgggtggaaa cccacagtaa
     1321 aacgtacggt tcgctctttc tttcagacac ctatggtctc ccactcaatc aaacgggcga
     1381 gagttgtagc accaatgatg aaaagattgc cacactcatc cgcctactaa gagaagcagc
     1441 gggcgagcat ggtgttttta atcagtctga acgtgactac cttggacgcg tactccgtgt
     1501 gtggagcgaa caccaagcgg ttcggctttc gtgaactgca gcgacgctga acttgtgcgc
     1561 cagccttggg caacctcggc tggcgcattt tttatacttt tcaaatacct ggtcgttgtg
     1621 tacgatgcct gaccaagggg aatgggcccc ggagcagagg aaatgtccct tttgttcacg
     1681 atgattgaag acgcggaacg acaagttcgc gcgcttcgag aaacaaactt gtgcgagccc
     1741 gagcatattc agccactgat tgagtggttg agggatcgcg aagaaaacga aggtctgata
     1801 cggtcaagcc gattggctga agcatttcag acggtcaatc cgacggtgtg ctcgcgctcg
     1861 ttggtctacc cgaccagaac gctccgcgaa acgcttgagg cgctttcggc gggcaaagac
     1921 aagagccaag acgtgttcga tttcacgctc aacgttctct cagcgtggtc agatatccag
     1981 tgcgcgcacg cataacgcac cctggtaaaa actataacgg cggccctcac aaggccgctg
     2041 ttttttatgc tattctccct cctatgacat cgctcgaaca taagcgccat acccttgccc
     2101 acctcctcgc tgccgccgtc cttgaaacgt acccagacgc aaagcgcact atagggccgg
     2161 cgatagatac gggcttctac ttcgactttg agttccctac cgaaaaacca acagaagaga
     2221 gcctgaagaa actcgaggca aagatgcgta agctcctacc gtcatggaaa agcatggagc
     2281 gcagtgagct ctccgctgaa gcggcaaaag ccgaatatcc agggaatgaa tacaagcacg
     2341 agctgatcga tgagttttca ggtgaaggac agacgctcac cttctacacg tcaggtcact
     2401 acagtgacct ctgccgtggt ggtcactgcg aaaatcctgc aaaggaaatc gcaggggact
     2461 ccttcatgct tgatcgcatc gccggtgcct actggcgtgg cgacgagaag aacccaatgc
     2521 tcacccgcat ctatggcctt gcctttgata ccaaagaaga actcgatgcg taccttgcca
     2581 accgcgaaga agcgaagaag cgcgaccatc gcaagattgg ccgagagcag ggtctctttg
     2641 tcttctccga tctcgtcggc tccggtatgc cgctcttcac ccctaaagga aacatcattc
     2701 gctcccaaat tattggattc tcacgtgaac tgaatgaagc acttggattc ggcgaagtgc
     2761 acaccccaaa cgtaaacaaa ggggagctct ttaagatttc cggtcactac gaccagtaca
     2821 aggaagacat gctctccgta cgcagccagt acgtggaaga tgagatgttc ctaaagccaa
     2881 tgaactgccc ccagcacacc cagatctacg cttctgcgcc gcgcagttac cgagaccttc
     2941 cggtacgctt ttctgacttc gctacccttt accgtgatga gcgtccagga gaactctccg
     3001 gtctcacccg tctacgcgcg ttcgctcagg acgatggtca catcttctgt cgtgaagatc
     3061 agatagaaga agagattagt gccgtgcttg ccgctattca gaaagcgctt gcggtgtacc
     3121 gcatcgacta ctggatgcgc ctctcacttc gggatcctca aaacaaagag aagtatttgg
     3181 gtgctgacga aggctgggta aaggcagagg ccatcatgcg aaagcttctc actgagaaga
     3241 acattacgca caaagaagca gaaggcgagg ccgcattcta tggaccaaag atagacatca
     3301 tggcggtaga tgcgcttggt cgtgagtggc aaatttccac catccagctc gacttcgtgc
     3361 agcctacgcg ctttcaactt gagtacacct ctgatgacgg tagtaagaag acgccggtga
     3421 tgattcatcg tgcacttgtc ggctctcctg accgtttcct cggtatattg attgaacact
     3481 acgctggcgc cttccctgcc tggcttgctc ctatacaggc gcgcattctc cctgtgtcag
     3541 acaaacatgc ggcgtatggt gctgagatac tcgcccaact taagggtgct ggtatccgcg
     3601 ctgaactgga tgacgcaaac gaatctcttg gaaaaaagat tagaaacgga aagacggata
     3661 aggtgccgta ccttctcgtg attggagacg cagaggtaga cgcaaagacg gtttccgtag
     3721 agagtcgtga tcacggaaaa cttgatgcaa tgaatattgc tgcatttatc gaacgcgtac
     3781 agaatgagat tgcgacgcgt gcttaacgcc ctcccgacaa cccttttacg atatcttcaa
     3841 cggtgagctc ttcgccctta gcaaacgaac gaaatccctc gtggcttgag tagcggggac
     3901 gcggcgtgtg ctgggccggg gcgtgatgtg ctttcggtgc gggagcatga tgctccacag
     3961 gggtagctac ttctttttcc gtgatgcgcg gcagaatctt aaagaggagc aggtacgaaa
     4021 tgacgccgct ccaaagagcg agcacaagga aataaagaat agcgtctacg gacattccac
     4081 tagtataccg aaaacggccc cgcagtgcgg ggccgttttc ttaataacct gtt
//
LOCUS       DAORIN010000055         4009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4787_length_4009_cov_5.329793, whole genome shotgun sequence.
ACCESSION   DAORIN010000055 DAORIN010000000
VERSION     DAORIN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4787_length_4009_cov_5.329793"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PK109_02655"
     CDS             <1..1063
                     /locus_tag="PK109_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRH24462.1"
                     /translation="TYSFEDRGGRRVTLRPEMTPSLARMVAAKRREMPFPLRWFSIGN
                     RFRYERPQRGRLRDFYQTDVDFVGLPAGESDLEVVTLASDILKSFGATDADFAIRVNS
                     RALLGAACRAVGLTDAEGVKAYWVLLDKKAKMSAEEFAAARGDKDPLKAIEDATDEAV
                     RVEKEKIEAFIATLKERGIENVSFDPEIVRGFDYYTGMVFEINDTHEENPRALFGGGR
                     YDGLVAMFGGDPIPAVGFAFGDVTFSDFLETHGLLPKIDTVPQVYIGTPEPTDAKAAQ
                     EFAATLRALGIRVFVNLSEKELGAQVKDAVKRGIPYFMAYGAQEAKSSMIRLKDLARS
                     EEESIEENQIGKHLAKLLK"
     gene            1065..1643
                     /locus_tag="PK109_02660"
     CDS             1065..1643
                     /locus_tag="PK109_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRH24463.1"
                     /translation="MRAITFDIEAISPNMVRGRLDVNEMELTVVGIHDSSTGEYSSFL
                     KDELPKLWPIIEQSDMLIGFNSDTFDIPLLNRYYPGDLTKMRSLDLLSEVYKTLGRRI
                     RLQSLAEATLGRGKSGDGLKATVWWQEGKYDLVREYCIEDVRLTRELYDYALTKGHLK
                     YKDLNQKKDIKLDTSRWEAEAEGHALTHTLGI"
     gene            1727..2038
                     /locus_tag="PK109_02665"
     CDS             1727..2038
                     /locus_tag="PK109_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24464.1"
                     /translation="MNKLRMEIHGKVNGVQLDALVDHFGTALAPTPHLPFSFRTITHI
                     PDTGPTTRIMVFVDELGQVDMVKLFSALNTIPVEADVNLGWSPRAPNVVELSAARKLA
                     G"
     gene            2113..2805
                     /locus_tag="PK109_02670"
     CDS             2113..2805
                     /locus_tag="PK109_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRH24465.1"
                     /translation="MDKNQNFLPGVIIIAGLLIAGAIFFVRSNAIVPTKGDVSLLRPV
                     STSDHLVGNPEAPVVVVVYSDIDCQYCKTFHEAMEQLTADYGPNGKVAWVYRHFPLID
                     VHPNAAAHAEAAECVASLGGSDLFFRFIDALHQAAPDADQFDPRGYASVVTSLGVSVT
                     DFDACIAGSKFEKRVADDYDNAIEMGAGGAPFSVILIKGHEPVPVSGSLPYLSLKKVI
                     DAAIEKAAEPAS"
     gene            complement(2802..3644)
                     /locus_tag="PK109_02675"
     CDS             complement(2802..3644)
                     /locus_tag="PK109_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRH24466.1"
                     /translation="MVKKKRTTKSQKLPIAYWSHSSLMAFLRNPLAWYKRYVEKIYDT
                     PRTPASIIGSAGHLALEHFYGGIDKEEAIKSGLDYMRAIADFEINFGVAKTRVAQKAK
                     RLSMERDYLQAIGFYLAKPPRHKVLGIEVRGIASVPGQKLPVKAISDLVVESKGNPGM
                     VDIVDHKFVDSFTKAPQTKTLFLIQAIFNYYTVSELYGKPICRFIVYECKKRKNADGK
                     PQMRKYVITYAEIGEIFEVFHRLLADATEEISRTRVFLPNPSDMFEGEDSFSLYRLRL
                     AEAD"
     gene            3777..>4009
                     /locus_tag="PK109_02680"
     CDS             3777..>4009
                     /locus_tag="PK109_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24467.1"
                     /translation="MTVRTLLSVVGGLLLVGFGGLVLSFTPHLWGTANELAAHGATDG
                     ESAMKLYLIGGMATGVALTGFGLFFVYRPFALTP"
BASE COUNT          930 a         1011 c         1085 g          983 t
ORIGIN      
        1 gacctactcc tttgaagacc gtggtgggcg ccgcgtgacg ctccgtcctg agatgacgcc
       61 gagccttgca cgcatggtgg ccgccaagcg ccgtgagatg cccttcccgc ttcgctggtt
      121 ctctatcggc aaccgcttcc gctatgagcg cccgcaacgt ggacgtcttc gtgatttcta
      181 tcagacggac gtggacttcg ttggcctccc tgccggcgaa tccgatcttg aagtggtcac
      241 cctcgcatca gacattctga aatcgttcgg tgcaacggat gcggacttcg ctattcgcgt
      301 gaactcccgc gcgcttctgg gggctgcgtg tagagcagta ggactcaccg acgctgaagg
      361 agtgaaggcg tactgggtat tgcttgataa gaaggcgaag atgagtgcgg aagaatttgc
      421 cgcagcacgc ggtgacaaag atccactcaa agcaattgag gacgccaccg atgaggctgt
      481 ccgcgtagag aaagaaaaga tagaagcatt tatcgctact cttaaagaac gcggtattga
      541 aaatgtctcc tttgatccag aaatagttcg tggctttgat tactacaccg gcatggtatt
      601 tgagataaat gacacgcacg aagagaaccc gcgtgctctt tttggcggtg gccgttatga
      661 cggacttgtc gctatgtttg gcggcgaccc aattcctgcc gtagggtttg cgtttggcga
      721 cgtcactttc tcagacttcc ttgaaaccca cggtcttctt ccaaagatag acacggttcc
      781 gcaggtatac atcggcacgc cagaacctac cgatgcaaag gcggcgcagg aatttgcagc
      841 tacactccgt gcgctcggta tccgtgtctt tgtaaacctt tctgaaaaag agcttggcgc
      901 tcaggtaaaa gatgcagtga agcgtgggat cccgtacttc atggcctacg gcgcacagga
      961 agcgaaatcc agcatgattc gccttaagga tttggcgcga agcgaagagg aatcgataga
     1021 agaaaatcaa attggtaagc atcttgcgaa gctcttgaag tagcatgcgg gcgattactt
     1081 ttgacataga ggcgatatcg ccgaacatgg tgcgcggcag actagatgtg aatgagatgg
     1141 aacttaccgt cgtcggcatt cacgactctt ctactggcga atactcttca ttccttaagg
     1201 acgagttacc gaaactctgg cccatcatcg aacagtcgga catgttaata ggattcaact
     1261 ctgacacctt cgacataccg ctcctgaatc gttactaccc cggggacctc acgaagatgc
     1321 gctcgttgga tcttttaagt gaggtgtata agacgttagg gcgccgtata cgattgcagt
     1381 cgcttgccga agcaacgttg gggcgcggga agtcgggaga cggtcttaag gcaacggtgt
     1441 ggtggcagga aggaaaatat gacctcgtac gcgaatactg cattgaagac gtccgcctca
     1501 cgcgcgagct ctatgactat gcgcttacca agggacattt gaagtacaag gatctgaatc
     1561 agaagaagga tattaagctt gatacctccc gctgggaggc agaagcggag ggtcacgccc
     1621 tcacccacac cttaggaatt tgaggttgat ttgaggctct gcctaggata ggatgccccc
     1681 ttacgcttcg caccgtgcga agcgtatgta cacgggaact agactcatga ataagctacg
     1741 catggaaatt catggaaaag tgaatggcgt tcagctcgac gcgctggtag accattttgg
     1801 taccgcgctc gcgccgacgc cccatctgcc gttcagcttc agaaccatca cccacatccc
     1861 tgacaccggc ccaacgacca ggattatggt ctttgtcgac gagctcgggc aggtggacat
     1921 ggtgaagctt ttttcggcgc tgaacacgat tccagttgaa gccgacgtca accttggttg
     1981 gagccctcgg gcgccgaacg tggttgaact ctctgctgct cgaaagctag cgggctaacg
     2041 aatacgtttc gctaatcacg tgcgcggcct tcacgggtcg cgcacgttca tgtacatgct
     2101 atcctttttc ctatggataa gaaccaaaac tttctcccgg gcgtcatcat aatcgccggt
     2161 ctccttattg caggcgccat atttttcgta cgcagtaacg ctattgttcc gacaaaggga
     2221 gacgtgtccc tgctgcgacc cgtaagcacg agcgatcacc tcgtaggaaa cccggaggct
     2281 ccggtggtag tcgtggtgta ttccgatatc gattgtcagt actgcaaaac tttccacgaa
     2341 gccatggagc agcttactgc tgattacggt cctaacggta aggtcgcctg ggtgtaccgt
     2401 cacttccccc ttattgatgt ccacccgaac gccgcagcac acgctgaagc ggctgagtgt
     2461 gtcgcctctc ttggtggatc tgatcttttc ttccgattca tcgacgcact ccaccaggca
     2521 gcgcctgacg cagatcaatt tgatccaaga gggtatgcgt cagtagtaac gagtcttggc
     2581 gtctcggtca cggactttga tgcgtgtatc gcgggaagca agttcgagaa acgcgttgca
     2641 gacgactatg ataacgcgat agagatgggc gcgggcggcg ctccctttag cgttattctg
     2701 attaaaggac atgaaccggt tcccgtctcc ggttctcttc cctatctttc tttaaagaaa
     2761 gtcattgatg ctgcgataga gaaagcagcg gagcctgcgt cctagtcagc ttccgctaga
     2821 cgcaagcgat acagcgagaa cgaatcttcg ccttcaaaca tatcggaggg gttcgggagg
     2881 aatacacggg tgcgagagat ttcttccgtc gcatcagcaa gcagccggtg aaagacttcg
     2941 aatatctctc ctatctctgc gtaggtaata acgtatttac gcatctgcgg tttgccgtca
     3001 gcgtttttgc gcttcttgca ctcatacacg atgaagcggc agatgggttt tccgtagagc
     3061 tcgcttacgg tgtagtagtt aaagattgct tggatgagga atagtgtctt agtctgtggc
     3121 gccttagtga acgagtcgac gaacttatga tcgacgatat ctaccatgcc agggttacct
     3181 tttgattcca ccaccaggtc agaaattgct ttgactggaa gtttctggcc tggaacggag
     3241 gcaatgccac gtacctcaat accaagcacc ttgtggcgtg ggggttttgc caagtagaac
     3301 ccgatggcct ggaggtagtc gcgttccata gaaagacgct ttgctttttg cgctacacgc
     3361 gtcttagcga cgccaaagtt aatctcaaaa tctgcgatgg cacgcatata atcaagacca
     3421 ctctttatgg cctcttcttt atcgatgccg ccataaaagt gttcgagtgc gaggtggccg
     3481 gcacttccta tgatagaggc gggggtacgc ggagtatcgt atatcttctc gacgtagcgc
     3541 ttataccagg caagcggatt acggagaaaa gccatcaagg aagagtggct ccagtacgct
     3601 atggggagct tttgtgattt cgtggttcgc ttctttttta ccatgtcact tcagtataca
     3661 aaatctctca gtgtaccttt gtttttattg acaaagacac tttatgatat ataatacgta
     3721 aacgaaggtg catctagcca tggtggcgaa cccgcaaggg agatgtaata cattccatga
     3781 ccgtacgtac attgctaagt gtggtaggtg gcctgctact ggtcggattc ggcggattag
     3841 tgctctcgtt cactccgcac ctttggggaa ctgccaacga actggcagcc catggtgcga
     3901 cagatggcga gagcgcaatg aagctctatt tgatcggcgg catggcgacg ggggtggcat
     3961 tgacgggttt cggccttttc ttcgtctatc gtcccttcgc actcacccc
//
LOCUS       DAORIN010000056         4000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4807_length_4000_cov_4.483143, whole genome shotgun sequence.
ACCESSION   DAORIN010000056 DAORIN010000000
VERSION     DAORIN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4807_length_4000_cov_4.483143"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PK109_02685"
     CDS             complement(<1..915)
                     /locus_tag="PK109_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24468.1"
                     /translation="MKRFTRLLGLFVISASVIAGAAFVPHLAAVHAQELTKEERAELE
                     DQYDKLQEEIGQWQQVLDATRAKKNTLQGDVTTLNAQIAQAEAQIKAKNIEIGKIGNQ
                     IKTKTARINELSAKIDDGHDSLASFLRRQDQVDDLSLVEIAFAADSAFSLFEDVDNIV
                     TVQSGLHKEFAEIREIRTETESERKALADQQSAAADARYVVETKKQEIDKNKADKNQL
                     LAITKNEEAAYSQVLAERQAQAAAIRARLFPLRDTDTIQFGDAARYAQAASAKTGVRA
                     ALILAILSQESSLGANVGNCLITDLTTGD"
     gene            complement(1004..1648)
                     /locus_tag="PK109_02690"
     CDS             complement(1004..1648)
                     /locus_tag="PK109_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRH24469.1"
                     /translation="MTITLSAVKREETGKASKALLSAGSMPAVVYGPKQEAVSITVAS
                     RDFEKILRDSGESTVIDLQGLGTPLQVLIHEIDRDPVTNVPRHADFYAIEKGAKVEVA
                     VPLSYVGESAAVKAGGSLVKVLHELEIESSPANLPQEIEVDISVLAAIGDQIHVSDLK
                     LPSGVEAKIEGDQIVALIQEVEVEAEETVAAPDMAAIEVEKKGKTEEEGGEAAA"
     gene            complement(1693..2694)
                     /locus_tag="PK109_02695"
     CDS             complement(1693..2694)
                     /locus_tag="PK109_02695"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class I"
                     /protein_id="HRH24470.1"
                     /translation="MIDLSSSAKSLCAVPKGILAADESDGTCDKRFAAQGIEQTDEMR
                     RAYRDLLLSTPGVEEYISGVILYKGTLEQNANDGTSFAASLLSRGIVPGIKVDEGTEP
                     MPESADELITKGLLGLSERLHGYRDAHKTGFTKWRAVVTIDGDRLPTSAAVVENAKRL
                     AMYARCVQEAGMVPMLEPEVLYDGMHSRMRSRAVITEAVSALMQALGEHGVDPSGVIL
                     KTSMALSGKGTGRIDSPEDVAEDTLGALLQAVPAVVPGIVFLSGGQTPDQATDNLRAI
                     AQLAKAKNAPWPLTFSFSRALQEEALALWKGKEENVPAARAAFLSRLDKVSKAAAGL"
     gene            complement(2718..3908)
                     /locus_tag="PK109_02700"
     CDS             complement(2718..3908)
                     /locus_tag="PK109_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24471.1"
                     /translation="MKRRESGSSNSTNPTTPITITWSSTNASSCVGTGFQTSGATSGT
                     VTESPTSTTQYIISCTSGSGTSASGGWEGDSWWTGGTQTTIYGETVHDNGGQGELCPN
                     AAFNAGYSAWNEEFVRYPNRCGDAGCDLAVVTCYGVTGATGTTNKPAEYNGGHCIAPL
                     GVGCRDTSYRRSSESAQEGAGVATDAETVTVTTTQCSDGISNDSDGLIDMADSGCTDP
                     GDTTEDPNAVTATLTNNGPIISGQSAMLTWSSTGATSCSSTSFATGGATGGSVSTGVL
                     TNPPQPSYPYSVNCSGAVANTTVLLTNPTAYIEANPDRVNVDATTLLSFSAAGLTPAS
                     SCTVTGPTGTLWTGSSDGAGALATTTSTSPKITQQSTFRISCDGATATDNVIVNLVPQ
                     FEEF"
BASE COUNT          857 a         1115 c         1077 g          951 t
ORIGIN      
        1 atcgcccgtc gtaaggtcag tgatgagaca gttgccgacg ttagcaccaa gagacgactc
       61 ttgagaaaga atcgccaaga tgagcgcggc acgtaccccg gtctttgcgg acgccgcttg
      121 agcgtaacga gccgcatccc cgaactgaat agtgtcggta tcgcgaagcg ggaacaagcg
      181 tgcgcgtatt gctgctgcct gcgcctgacg ctcagcaagc acttggctat acgccgcttc
      241 ttcgtttttc gtaatcgcaa ggagttggtt cttatcagcc ttattcttat caatttcctg
      301 cttttttgtt tcaactacgt agcgggcatc cgctgctgct gactgctggt cggcaagtgc
      361 tttacgctca ctttcagtct cagtacggat ctcacgaatc tcagcgaatt ccttatggag
      421 tcctgactgt accgtcacga tgttatcgac gtcttcaaag agagagaatg cactgtcagc
      481 ggcgaatgct atttcaacta gcgagaggtc atcgacttga tcctgacgtc gaaggaagga
      541 ggcaagggaa tcgtgcccat cgtcaatctt tgcagagagc tcattaatgc gtgcggtctt
      601 tgtctttatc tgattcccta tcttcccaat ctcaatattc ttcgccttta tctgcgcctc
      661 ggcctgagca atctgcgcat taagggtcgt cacgtcaccc tgaagcgtat tctttttcgc
      721 acgggtggca tcaagcactt gctgccactg tcctatctct tcctgaagct tatcgtactg
      781 gtcttcaagc tcggcacgct cttctttagt gagttcctgt gcatggactg ccgcaaggtg
      841 cggcacgaag gctgctccgg caataacgga ggccgaaatg acgaacaatc caaggagccg
      901 cgtgaagcgt ttcatcggca tacagtatat cagagcgaaa acgggcctaa atatgacaga
      961 aaaaccgact ctcgccggtt gttcctcgca ctgtatagcg ggcttatgcc gctgcttcac
     1021 ctccctcttc ctcagtctta cccttcttct ctacctcaat tgcagccata tcaggagcag
     1081 caaccgtttc ttcagcttcc acttcaactt cctggatgag ggcgacaatc tggtctcctt
     1141 caatcttagc ctcaacgcct gatggaagtt tgaggtcgct cacgtggatc tgatccccga
     1201 tagcggcaag gacggagatg tctacctcaa tttcctgcgg aaggtttgct ggagatgatt
     1261 caatctcaag ctcatggagt accttcacga gtgagccacc cgccttcact gcagcagact
     1321 cgccgacata gctgagagga acagcaactt caaccttcgc tcccttctca atggcataaa
     1381 agtcagcgtg gcgagggacg ttcgtgacgg ggtcacgatc aatctcatgg ataaggacct
     1441 ggagcggggt accaagaccc tggagatcga taacggtgga ctctcctgaa tcgcggagga
     1501 tcttttcaaa atcgcgggac gctaccgtta tggatactgc ttcctgctta ggtccgtaca
     1561 ccactgccgg catagagcct gcagacaaaa gggcctttga ggccttgccg gtttcttcgc
     1621 gtttaaccgc ggacaacgta atagtcatga tgaacggcag tatgccctaa aggaggccgt
     1681 ggtgcaaggc ggctagagcc ctgccgccgc cttagaaacc ttatcgagac ggctgaggaa
     1741 cgccgcgcgg gctgccggga cgttctcttc tttccctttc cagagagcga gtgcttcttc
     1801 ctggagggca cgtgagaagg agaatgtgag gggccatgga gcattcttcg ccttcgcaag
     1861 ctgtgcgata gcacgcagat tatccgtggc ctggtccgga gtttggccgc cagagaggaa
     1921 cacaatgccg ggaaccactg ccgggaccgc ttgaagcaag gcgccaaggg tgtcctcagc
     1981 tacgtcttct ggagagtcga tgcggcccgt accttttcct gaaagcgcca ttgaagtctt
     2041 gagaataacg ccggaagggt cgacgccgtg ctcacccaac gcctgcatga gcgcagacac
     2101 cgcttccgtg ataacagcac gactcctcat acggctatgc ataccgtcat acaagacttc
     2161 aggctcgagc atcggcacca tgcctgcttc ctgcacgcag cgtgcataca tcgcaaggcg
     2221 ctttgcgttc tcaaccactg cagcagacgt cggaagacga tcgccatcga tggtcactac
     2281 cgcgcgccat ttggtgaacc cagtcttgtg cgcatcccta tacccatgca gacgttctga
     2341 gagaccgaga aggcctttcg tgatgagctc gtccgcgctt tctggcattg gctcagtgcc
     2401 ctcgtccact ttgatgccgg gaactatgcc tcgagacaga agtgatgcag caaacgatgt
     2461 accatcattc gcattctgtt cgagcgtacc tttataaagg atgacgccag agatgtactc
     2521 ttctacccca ggagtagaga gaaggaggtc gcgatacgcg cggcgcatct cgtccgtctg
     2581 ctctatacct tgggcagcaa accgcttatc acacgtaccg tcactttcgt cagcggcaag
     2641 tattcctttt ggcaccgcgc acagcgactt tgcggaagat gagaggtcaa tcatagccca
     2701 aattctagca cgaacgacta gaactcttcg aactgaggga caagattcac aatgacgtta
     2761 tcagtcgccg ttgcgccatc acacgatatt cggaaggtgg actgttgcgt gatttttgga
     2821 gaggttgagg tcgtcgttgc aagagctcct gcgccatcag acgaaccggt ccagagagtg
     2881 ccggttggtc ccgtaactgt gcacgaggaa gcgggagtaa gtccggccgc actgaacgag
     2941 aggagcgtag tagcgtccac gttcacgcgg tcagggttcg cttcgatgta agcggttggg
     3001 ttggtaagta ggacggtcgt gttcgccact gctcccgagc agttcactga gtacgggtag
     3061 cttggctgag gcggattggt aaggacgcca gtggaaaccg atcctccggt tgcgcctcca
     3121 gttgcgaagc tggtactgct gcatgacgta gctcccgttg aagaccaggt aagcatcgcc
     3181 gactgtcccg agataatagg gccattgttt gtgagcgttg ccgttacagc atttggatct
     3241 tccgtagtat cgcccgggtc agtacagccg ctatccgcca tatcaatgag cccgtcagag
     3301 tcgttcgaga taccgtctga gcactgcgtg gtagttaccg taacggtttc agcatcggtt
     3361 gcgactcctg caccctcctg agcggattcg ctcgagcgac gataggaagt atcgcgacac
     3421 cccacaccaa gtggtgcgat gcaatgaccg ccattatatt cagcaggctt gttcgtcgtg
     3481 cctgttgcgc cggtcacacc gtaacaggtg acgactgcca agtcgcaccc tgcatcgcca
     3541 cagcggttcg gataacgaac aaactcttca ttccaagctg agtagccggc gttgaatgct
     3601 gcattcgggc acagttcgcc ctgtccgccg ttatcatgca cggtctcgcc atagatggtc
     3661 gtctgcgtcc cgccggtcca ccaactatct ccttcccagc cgccgctcgc tgatgtgccg
     3721 gagccgctgg tgcaagaaat gatgtactga gtcgtcgaag ttgggctctc agtgacggtg
     3781 ccacttgtcg ctccgctcgt ttggaatccg gtaccgacgc atgaagatgc gttggtcgaa
     3841 gaccaggtaa ttgttatcgg agttgttggg tttgttgagt tgcttgatcc gctctctcgc
     3901 ctcttcaagc tgtgctttgt agtagtcgat tccgagcggt tccgatccgc caaactcgct
     3961 tgttgataca gccttctcct ccggcatggc gtaaacacaa
//
LOCUS       DAORIN010000057         3986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4840_length_3986_cov_3.973798, whole genome shotgun sequence.
ACCESSION   DAORIN010000057 DAORIN010000000
VERSION     DAORIN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4840_length_3986_cov_3.973798"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="PK109_02705"
     CDS             <1..1636
                     /locus_tag="PK109_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRH24472.1"
                     /translation="VVSREEITLIDASTYQMFSAATAMIPFVDHDDANRALMGSNMQK
                     QATPCLIPEAPLVATGIEEHAARNTGRLIIAIEAGEVTFADAKKITVENKDGKKKDYK
                     LQGLTQTNQNSAFSQRPIVTVGTKVKKGDVLADASSSANGQMALGQNVRVAFMSWSGA
                     NYEDAIVISERLVQDSKFTTVHIEDFEAAVRDTKLGAEITTHDIPNVGELRLKNLDEE
                     GVVRIGAEVRPGDILVGKVTPKGETQLTPEERLLRSIFGDKAKDVKDTSLRMEGGKRG
                     RVIGVKVFSREKGDQLESGIIKKIVVTVAQVRNVSVGDKLAGRHGNKGVISRVLPAED
                     MPYDKEGNPIDLILTPLGVPSRMNLGQILELHLGLAANTLGYQAIVPPFAGATADEIR
                     NELELAGHDRSGKMQLFDGRTGEAFAQAISVGYMYILKLHHMVEDKIHMRSIGPYSLI
                     TQQPLGGKAQNGGQRFGEMEVWALLGYGSAYTLREMLTIKSDDIQGRSATFDAIVKGE
                     PVTHSGTPASFSVLTNQIRGLALDIEPVEKPVDADA"
     gene            1648..>3986
                     /gene="rpoC"
                     /locus_tag="PK109_02710"
     CDS             1648..>3986
                     /gene="rpoC"
                     /locus_tag="PK109_02710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRH24473.1"
                     /translation="MATSNNPKRMMGPRGADWNALRLSLASPERILEWSRGEITKPET
                     INYRTQRSEKHGLFDEKIFGPEKDYECYCGKYKGIRYKGIICDKCGVEITRSIVRRER
                     MGHIELVTPVAHIWFLRSMPSRMALILGIPGADLEKVIYFAGYIVTKVNEDTKKSLLS
                     DLESEYKTKHKEAKDDKERDALKERMTLTKAEIDSVVVGRVFDEIAYHRYSVKYSTLF
                     EAKIGAEAIYDIFRNVKLDELKAVLEAKYANAASAEREKLAKRISLIASMIASKVRPE
                     WMFLTKIPVIPPALRPMVALEGGRHATSDVNDLYRRVINRNNRLKKLIDIHAPEVILR
                     NEKRILQEAVDALLDNSIRKSGASGGALTPSQRRPLKSLADYLKGKQGYFRQNLLGKR
                     VDYSGRSVIVVGPDLELDECGLPKHMALELFRPFVIAGLLSRELAFNIRGAGRLIEDG
                     VPEVWEILEEAIKGKHVLLNRAPTLHRQGIQAFRPRLIEGDAIQLHPLVCPAFNADFD
                     GDQMAIHVPLSEEAQWEAKNVMSANKNILKPGSGEMIVMTQKPLDIVLGAYWLTKDVP
                     DAKGAGKYFQSPNAAILAKDYDVVDIRAKIHVLPMADKEKYSMFEGKPFETTVGRLLF
                     NTVLPSDYPFINEPITKKVLSSVMADCMLRYGIDAVPGIVNKVKRFGFEYATRSGISW
                     SLDDVSVPEAKESVIDAARTKVNKIESDFRDGLLAAEEKRRMVIEVWHGAKTEVEKLV
                     EDALDPKGSVHDMVRSGARGSIGNLTQMAGMKGLIQNTAG"
BASE COUNT          950 a         1110 c         1082 g          844 t
ORIGIN      
        1 agtggtttct cgtgaagaga tcacgctcat cgacgcttct acctaccaga tgttttctgc
       61 agcgactgca atgattccgt ttgtggacca cgacgacgcg aaccgtgcac tcatgggttc
      121 gaacatgcag aagcaggcga cgccgtgtct catccctgag gccccgctcg ttgctacggg
      181 tattgaagag catgcggctc gtaacactgg acgcctcatc atcgctattg aagcaggtga
      241 agtgaccttt gctgacgcaa agaagatcac ggtagagaac aaggatggta agaagaagga
      301 ctacaagctc cagggcctta ctcagaccaa ccagaactca gcattcagcc agcgtcctat
      361 cgtcacggta ggtacgaaag tgaagaaggg agatgtcctt gctgacgcat caagttctgc
      421 taacggccag atggcactcg gacagaacgt tcgcgttgcc ttcatgagct ggagcggagc
      481 gaactacgaa gacgctatcg tcatctctga gcgcctcgtg caggattcca aatttactac
      541 cgttcatatc gaagacttcg aagctgcggt acgcgacacg aagctcggtg ctgaaatcac
      601 cactcacgac attccgaacg tcggtgagct ccgccttaag aacctcgatg aggaaggcgt
      661 cgtgcgcatc ggtgcagaag tacgtccggg cgacattctc gttggtaagg taacgccaaa
      721 gggcgagacc cagctcacgc cagaagagcg tctcctccgt tccatcttcg gcgacaaggc
      781 caaggacgtg aaggacacca gcctccgcat ggaaggcggc aagcgtggac gcgttatcgg
      841 ggtgaaggtc ttctctcgcg agaagggtga tcagctcgaa agcggcatca tcaagaagat
      901 tgtggtcacc gtcgcgcagg ttcgtaacgt ttccgttgga gacaagctcg ccggacgcca
      961 cggtaacaag ggtgttatct cccgcgtact tccagctgaa gacatgccgt acgacaaaga
     1021 gggaaatcct atcgacctta tcctcacccc gctcggcgtt ccgtcgcgca tgaacctcgg
     1081 acagattctt gagctgcacc tcggtcttgc cgcaaacacc ctcggatacc aggctatcgt
     1141 tcctccattt gcaggagcaa cggctgacga aatccgcaac gagcttgagc tcgcagggca
     1201 tgatcgctct ggaaagatgc agctcttcga tggtcgcacc ggcgaagcat tcgctcaggc
     1261 aatctccgtt ggctacatgt acatcttgaa actccatcac atggtggaag acaaaatcca
     1321 catgcgttcg attggtccgt acagcctcat cacgcagcag ccgcttggcg gaaaggcaca
     1381 gaacggtggt cagcgctttg gagaaatgga agtgtgggcg ctcctcggtt acggttctgc
     1441 ctacacgctt cgcgaaatgc ttaccatcaa gtctgacgat attcagggcc gctccgcgac
     1501 cttcgatgcc atcgtgaagg gtgagccggt gacccattcg ggaacccctg cttccttctc
     1561 ggtacttact aaccagatcc gcggcctcgc gctcgatatc gagccggtag agaagccggt
     1621 agacgcggac gcataaccta aatctctatg gctacctcaa acaatccgaa gagaatgatg
     1681 ggcccccgcg gcgcggactg gaacgcactc cgtctttcgc ttgcgtcccc tgaacgcatc
     1741 cttgaatggt cccgcggtga aatcaccaag ccggagacca tcaactaccg cacccagcgt
     1801 tctgaaaagc atggtctgtt cgacgaaaag atattcggtc ctgaaaagga ttacgagtgt
     1861 tactgcggta agtacaaagg catccgctac aagggcatca tctgcgataa gtgtggtgtg
     1921 gaaatcaccc gttctatcgt gcgccgtgag cgcatgggcc acatcgagct cgtcacgcca
     1981 gtcgcgcaca tctggttcct ccgctccatg ccaagccgta tggcgcttat cctcggcatc
     2041 ccgggggctg accttgagaa ggttatctac ttcgccggct acatcgtcac caaggtgaac
     2101 gaagacacga agaaatcact cctttcagat cttgaaagcg agtacaagac caagcacaag
     2161 gaagcgaagg atgataagga gcgcgacgct ctcaaagagc gcatgacgct tacgaaggct
     2221 gaaattgatt cagtcgtcgt tggccgtgtg ttcgatgaaa tcgcgtacca ccgctactcc
     2281 gttaagtact caacgctctt cgaagcaaag atcggtgctg aagctatcta cgatatcttc
     2341 cgcaacgtta agcttgatga gctgaaggca gtacttgaag caaagtacgc taacgccgca
     2401 tcggctgaac gcgagaagct cgcaaagcgc atctcgttga ttgcttcgat gattgcttca
     2461 aaggtacgtc ctgaatggat gttcctcacg aagattccgg ttatcccacc agcacttcgt
     2521 ccgatggtgg cgcttgaagg tggtcgtcat gcaacgtctg acgtcaacga cctctaccgc
     2581 cgcgtcatca accgcaacaa ccgtttgaag aagctcatcg acattcacgc tccagaagtg
     2641 attttgcgca acgaaaagcg cattctccag gaagcagttg atgcactcct cgacaactcg
     2701 attcgtaagt cgggtgcgtc aggtggtgcg ctcactccga gccagcgccg tccattgaag
     2761 tcacttgctg actacttgaa gggtaagcag ggctacttcc gccagaacct tctcggtaag
     2821 cgcgtcgact actctggccg ctccgtgatt gtcgtgggtc ctgaccttga gcttgatgaa
     2881 tgcggtcttc caaagcacat ggccttggaa ctcttccgtc cattcgttat tgctggtctt
     2941 ctttcccgcg agcttgcgtt caacatccgt ggtgcaggtc gtcttatcga agacggcgta
     3001 ccagaagtct gggaaatcct cgaagaagca attaagggta agcacgtgct cttgaaccgc
     3061 gccccaacgc ttcaccgcca gggtatccag gcattccgcc ctcgccttat cgaaggtgac
     3121 gctatccagc ttcacccgct cgtctgtcct gccttcaatg cagacttcga cggcgaccag
     3181 atggctatcc acgtaccgct ctcagaagag gcgcagtggg aggcaaagaa cgtcatgtcg
     3241 gcgaacaaga acatcttgaa gccaggttcc ggagaaatga ttgtgatgac ccagaagccg
     3301 ctcgatatcg tactcggcgc ctactggctc acgaaggatg ttccggatgc aaagggcgca
     3361 ggaaagtact tccagagccc gaacgctgcg attcttgcga aggactacga cgtcgtcgac
     3421 atccgcgcaa agattcacgt gcttccgatg gccgacaagg agaagtattc gatgttcgaa
     3481 ggaaagccgt ttgaaaccac ggtgggacgc ctcttgttca acaccgtgct cccttcagac
     3541 tatccgttca tcaacgaacc aatcacgaag aaggtgctct caagcgtcat ggcagactgc
     3601 atgctccgct acggcattga tgcggttccg ggcatcgtga acaaggtgaa gcgcttcggc
     3661 ttcgagtacg caacacgctc cggtatttcc tggagccttg atgacgtatc ggttcctgaa
     3721 gcaaaggaat cggttatcga cgctgcgcgc acgaaggtga ataagattga aagcgacttc
     3781 cgcgacggtc ttttggctgc agaagagaag cgccgcatgg tgattgaggt ctggcacggt
     3841 gctaagactg aggtagaaaa gctcgttgaa gacgcacttg atccgaaggg ctccgtgcac
     3901 gacatggtgc gctcgggtgc tcgcggttcc atcggtaacc tcacgcagat ggccggtatg
     3961 aagggtctca tccagaacac ggccgg
//
LOCUS       DAORIN010000058         3972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_4879_length_3972_cov_5.482002, whole genome shotgun sequence.
ACCESSION   DAORIN010000058 DAORIN010000000
VERSION     DAORIN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_3972_cov_5.482002"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1360
                     /gene="purD"
                     /locus_tag="PK109_02715"
     CDS             92..1360
                     /gene="purD"
                     /locus_tag="PK109_02715"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRH24474.1"
                     /translation="MNILVLGSGGREHALSWKIAQSPLVQKLYIAPGNAGTAQVGENV
                     ALTITDTSAVAAFCKEQNIGLVVVGPDDLLAVGIVDALETEGITVFGPSKAAAQIEWS
                     KAYAKQVMQEEGIPTARHRVFESAEGALAYAEKQPLPLVIKADGLALGKGVVIATTHE
                     EAAAAINDFMGKKIHGDAGATVVIEEYMEGLEISVHALCDGERALMFPSSKDHKRVRE
                     NDEGPNTGGMGTVAPVPIVPAAQMQLVEERIVLPLLAALKKRGTPFKGLLFPGVMLTK
                     YGPMVIEFNARFGDPETQSYMRLMESDVVPVLFAAAKGDIGDIRLTWEEGAAACIVMA
                     SGGYPGAYEKGKEIAGIENAEANDCVVFHAGTKRDEDGTLRTNGGRVLNVTALGKELP
                     EALHRAYEGVATIQFEGAQHRTDLGKKAIP"
     gene            complement(1357..1785)
                     /locus_tag="PK109_02720"
     CDS             complement(1357..1785)
                     /locus_tag="PK109_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRH24475.1"
                     /translation="MELAGATHAGGVVIGDGGTIALVYRKTTGVWTFPKGKIEADETD
                     EAAALREIAEETHLTDLEYIDDLGSYTRTKGGVEGAPHKTIHLFLYAAPRAALLTPAH
                     EITETAWVPYREVASRLTIDKDRAWYATVFDRVREAIQRD"
     gene            1846..2850
                     /locus_tag="PK109_02725"
     CDS             1846..2850
                     /locus_tag="PK109_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRH24476.1"
                     /translation="MTKLDIIVDLQYGDSGKGKVAHFLCREKKYTHVLRYNGGANAGH
                     TIFHKGVKFVTHQIPVGVFFGVKSIIGPGCVVDPSVLMQEVKELEKHGIKVKGKLFVA
                     HNAHVVTPAHKKEDGTDKKIGTTKRGIGPAYRDKFSRTGVRAESIPSLKPYLIDLYKE
                     FEGGSKEATVLCEGAQGFGLDIDWGDYPFVTSSHCTSGGAIMNGFAPKTVRDVWGVAK
                     VYETYVGNKKFEPAGAIFKKLRAAGEEYGATTGRPRQCNWLDIELLERAVKLNGVNHL
                     VLNKIDVLDAVSAWKIREGKKVQSFKDGKTMRAYIVKRLKKSGISGKNIHFSNRKDGV
                     "
     gene            complement(2860..3414)
                     /locus_tag="PK109_02730"
     CDS             complement(2860..3414)
                     /locus_tag="PK109_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24477.1"
                     /translation="MKPEGVPETPKSTGIEHSTQDRHAEHSDIENETPLPEPEIPQGE
                     RSPESEQIRQVRRHLETQSTHATLLKKVEKDEQMRTAMSAVSSEHSKRMDAIDRNNEG
                     VRKAMNRVGALGVLTGVIGTFFITGYGFGLVVGGAVVVTGCGVGRMINAIRTRIHQGQ
                     EQRRWDRAIQEIRTKYENMPDRGY"
     gene            3642..3806
                     /locus_tag="PK109_02735"
     CDS             3642..3806
                     /locus_tag="PK109_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24478.1"
                     /translation="MTIEKFGGGRGNWSQEDKDRILREKEELQTENRKRRANELGQDY
                     DEMFPSENKG"
BASE COUNT          974 a          971 c         1065 g          962 t
ORIGIN      
        1 gtcgccgtct gctgactgca tcattcctga agtacttgat gaacgcgtgg tgcctaccat
       61 tgcggcagcg gtacagtgat accatacgcg catgaatatt cttgtcttag gaagcggcgg
      121 acgtgagcac gcactttcat ggaaaattgc gcagtcaccg ctcgttcaaa agctctatat
      181 cgcgccggga aatgcaggca ccgcgcaggt aggggagaat gtcgctctca ccatcacgga
      241 cacgagtgcc gtcgctgcat tttgtaagga acagaatatt ggactcgtcg tcgttggccc
      301 cgacgatctt cttgctgttg gcatcgttga cgcgcttgaa acggaaggaa ttacggtctt
      361 tggcccatcg aaagcagcag cacaaattga gtggtcaaaa gcctacgcaa aacaggtgat
      421 gcaagaagag ggtataccca ccgcacggca ccgcgtgttt gaaagcgcgg agggtgcgct
      481 tgcgtatgcc gagaagcaac cgctcccgct cgtgataaag gcagatgggc tggcgcttgg
      541 taagggagtc gtcatcgcta cgacgcatga agaagccgct gctgccatca atgacttcat
      601 gggaaagaag attcatggcg acgcgggtgc caccgttgtt atcgaagagt atatggaggg
      661 acttgagatt tccgtgcacg cgctttgtga cggagaacgt gcgctcatgt ttccctcgtc
      721 caaggatcat aagcgggtgc gtgaaaacga tgagggacca aacaccggcg gcatgggaac
      781 ggtagcaccg gttcccattg ttccagcagc acaaatgcaa ctggttgaag aacgcattgt
      841 tcttccgctt cttgccgccc ttaagaagcg cggtactcca ttcaagggac ttcttttccc
      901 gggagtgatg ctcaccaagt acggccccat ggtcatcgaa ttcaacgcac gctttggtga
      961 cccggaaact cagtcctaca tgcggctcat ggaaagtgac gttgttcccg tactttttgc
     1021 tgcggcaaaa ggtgacatag gagatatacg cctcacctgg gaagaaggag cggcagcgtg
     1081 catcgtaatg gcatcaggtg ggtatcctgg cgcgtatgaa aaaggaaaag agatagccgg
     1141 catagaaaac gcagaggcga acgactgcgt ggtatttcat gcaggcacga agcgagacga
     1201 agacgggacg cttcgcacga atgggggacg cgtgctgaac gttacggcgc tcggtaaaga
     1261 gttaccggaa gcgctccacc gcgcatacga aggagtcgct acgattcagt ttgagggagc
     1321 gcagcatcgc actgatcttg gaaagaaggc aatcccttag tcgcgctgta ttgcttcacg
     1381 aacgcggtca aacaccgttg cataccatgc acggtcttta tcgatggtga gtcgtgatgc
     1441 aacttcacgg tacggtaccc acgcggtttc tgttatctca tgtgcagggg taagaagcgc
     1501 ggcgcgcggc gcagcataca agaaaagatg aatcgttttg tgaggtgcgc cctcaacgcc
     1561 tcctttggtg cgcgtgtagc tcccaaggtc gtcaatgtac tcaagatcgg tgagatgtgt
     1621 ttcctccgct atttcacgga gtgccgccgc ttcatctgtt tcatccgcct caatcttccc
     1681 cttaggaaag gtccagacgc ccgtggtttt cctatacacc agtgctatcg tgccgccgtc
     1741 tccgatgacg acgccccctg cgtgcgttgc tccagctagt tccatgggcg gtagtatgac
     1801 atatcccttg cgccttaagg gcagggacgt gataatggcg gagctgtgac caaactcgat
     1861 atcatcgttg accttcagta cggagattca ggaaagggca aggtagccca ttttttatgc
     1921 agagagaaaa aatacacgca cgttcttcgc tataacggcg gcgcaaacgc gggacacacc
     1981 attttccata aaggcgtaaa gttcgtcacg caccagatcc ctgtcggcgt cttctttggc
     2041 gtgaaatcca tcatcggtcc cggctgcgtc gttgatccgt cggtgctgat gcaggaagtg
     2101 aaagaactcg agaagcatgg catcaaagta aaagggaagc tcttcgtcgc acacaatgcg
     2161 catgtagtga cgccagcgca caagaaagaa gacggtacgg ataaaaagat tggtaccact
     2221 aagcgcggca ttggtcctgc atatcgcgat aagttctcgc gcaccggtgt gcgtgcagaa
     2281 tcaattcctt ctctcaaacc gtacctcatt gatttgtata aagagtttga gggtggttcc
     2341 aaagaagcaa cggtgctctg tgaaggcgcg caaggtttcg gtcttgatat cgactggggc
     2401 gactatcctt tcgtcacttc aagtcactgc acgtcaggtg gcgccatcat gaacgggttt
     2461 gcaccaaaga ccgtgcgcga tgtgtggggc gtcgcgaaag tgtatgagac gtatgtgggg
     2521 aataagaagt ttgaacctgc gggcgctatc ttcaaaaaac tccgcgcagc aggagaggaa
     2581 tatggcgcga ctaccggacg cccgcgccag tgcaattggc ttgatattga actgcttgag
     2641 cgtgccgtaa aactaaacgg cgtgaatcat ttggtactga ataagataga tgtgctggat
     2701 gcggtaagcg catggaagat acgcgaaggg aagaaggtgc aatcgtttaa ggatgggaag
     2761 acgatgcgtg cgtatattgt gaagcgtctt aagaagtcag gtatctctgg aaaaaacatt
     2821 cacttttcta acagaaagga tggggtgtaa gagatagtcc tagtagcctc ggtccggcat
     2881 attctcgtac ttcgttcgta tctcttgtat cgctctatcc catctacgtt gttcttggcc
     2941 ttgatgtatt ctcgtccgta ttgcattgat catacgcccc accccgcacc cggtgactac
     3001 cacagctccc cctactacta acccgaatcc gtatccggtt ataaagaacg taccgataac
     3061 tccggttaag acgccgagcg cccctactcg attcattgct ttgcggaccc cttcattatt
     3121 tctatcgata gcatccattc gttttgaatg ctcggacgac acggcactca tagcagttcg
     3181 catctgctca tccttttcta cctttttcag aagggtagcg tgcgtgctct gtgtttcaag
     3241 atgccgtctc acttgtctga tttgttcact ttctggcgac ctctctccct gcggtatttc
     3301 tggttccggt aagggtgttt cgttctctat atcagaatgt tctgcatgcc gatcttgagt
     3361 ggaatgctct attccggtac tttttggcgt ttcaggtacc ccctcgggct tcatagtgaa
     3421 ggtaagtata cctttgattc aaaagggcaa tatcagaatc ttgatagtcg gagacggaag
     3481 gacgctaacc ttgaagtgct catacgcacg gctaacctga cctgtcttat tcagacgctg
     3541 ccgctttgtt tcggtaagac accagcacta ctgttgtgag tgggcactat acccaccggg
     3601 accgttgact tgtaagtttt tgattttaaa atcaagcgta tatgaccata gaaaaatttg
     3661 gtggtgggcg tggtaactgg tctcaagaag ataaggatcg aatacttcgc gaaaaagagg
     3721 agcttcaaac agaaaacaga aaaagacgtg ccaatgagct tggtcaagac tatgacgaga
     3781 tgttccctag tgaaaacaaa ggctaacgta tagcagctct cttggttccg taaataagat
     3841 aattagttac aacaaaaagc acaggaccct gtgctttttg cttattctag aagatggcac
     3901 accgtgccat ctgttaattg cggtgaaatt atatcccttg tccgaaccca tttcgagcgc
     3961 cagagccgct cc
//
LOCUS       DAORIN010000059         3829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_5215_length_3829_cov_4.533121, whole genome shotgun sequence.
ACCESSION   DAORIN010000059 DAORIN010000000
VERSION     DAORIN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_3829_cov_4.533121"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="PK109_02740"
     CDS             <1..1959
                     /locus_tag="PK109_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRH24479.1"
                     /translation="RMSRVRTSDRFRAMSATTWKNLWKNKASTPESGNAREQQCARVF
                     SFPRVPYIGSLSHCVGTLYLRVAMKVSEKELKEFILDSGLVPRKDLEAAEVEAEKRKQ
                     SVGDILLASGALTQDALRRIKAYVLGIPFINLKDRKVPVEILSLIPEPIARTHNIIGF
                     RKENGELEVAMLDTEDLPAIDSVRKKTGLKILPRLTDEESIKHALLQYQKSLKEEFGD
                     IISNEAGKINLIKDAGIGGEEMSGDELKKMAEDLPIVRIVDTLLRHAITQDASDIHIE
                     PMEMEVLVRYRIDGILHDAMTLPRAAAPGITARIKVLSNLKLDEKRLPQDGRFKMETE
                     ADKVSFRVSLLPTYYGEKVVMRLLRETGEGFTLEALGFHGEPLERLHKATKATTGIIL
                     VSGPTGSGKTTTLYTILDILNTPDVNISTVEDPIEYQMKRVNQTQVNPTIGFSFANGL
                     RALLRQDPNIIMVGEIRDEETASLAINAALTGHLVLSTIHTNSATGAIPRLLDMGVEP
                     FLLVSTLRVVVGQRLVRRLADGKEQYTLTKAEREKVASEDHFDAVMKALAEEKIVKAG
                     TKIDAVPFYRPKAGGETGDGYKSRIGIHEVLQVTPSLREMIMKSATSDQLEEQARKEG
                     MLTMLEDGMYKASRGITSLEEVLRAITE"
     gene            2051..2410
                     /locus_tag="PK109_02745"
     CDS             2051..2410
                     /locus_tag="PK109_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24480.1"
                     /translation="MSKLTSAVLFGFAVAVVTFFFSDSLIMWSGGKAHENIVLTTVIY
                     GFLAMLSVYWPKSSAVLAFFLIFLTGLLVVVLGESQSGRVEKVFVVILHALPFFLPLL
                     WSTGYFDLFRNAKRTSA"
     gene            2493..3707
                     /locus_tag="PK109_02750"
     CDS             2493..3707
                     /locus_tag="PK109_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRH24481.1"
                     /translation="MKFTVTIKKDDGAEEKRVVEAPTRFAVYADMEKEGATVLALSEG
                     GGGFTMPAWANIQLTSGVKTEERITFTKNLSAMLGAGLTLSRALSVIDRQSANKGLKR
                     IVADLEARVKKGDAFNEALASHPKVFSPLFIAMTKAGEESGTLADALKVVAKQMDRSF
                     TLQKKIKGAMIYPAIILFAIAIIGVLMMVYVVPTLAATFKELGAQLPAATLAIMSISE
                     FMSSHVILVFVILFASIFAVIAFLKTKIGGNIVLAGALHMPVIGTLVRETYSARAARA
                     MSSLLSSGVEMLSAISITEEIVGDNRFGKVVAEASARVKKGDALSAPFIEHPKLYPVF
                     FGEMIAVGEETGQVAGMLAQVAEYYENDVEEGTKDLSTIIEPLLMLFIGAFVGVFAVS
                     MIAPIYSLSSQI"
BASE COUNT          917 a          979 c         1013 g          920 t
ORIGIN      
        1 cgtatgtccc gcgttcgaac ctcggacaga tttcgtgcga tgtccgcaac gacctggaag
       61 aacctgtgga agaataaagc gtcgacgcca gaaagtggga acgcccgcga gcagcaatgc
      121 gcgcgggtgt tttcttttcc acgcgtacca tatataggaa gtctctctca ttgcgtcggc
      181 acgctatact tacgcgtagc tatgaaggtc tccgagaaag aactaaagga attcatcctc
      241 gattcggggc tggtgccgcg caaggatctt gaggcagcgg aagttgaggc ggagaaacga
      301 aaacagtccg tgggagacat tctccttgcg tcaggcgccc tcacgcaaga cgcgcttcgc
      361 cgcatcaagg cgtacgtgct tggtattcct tttattaact tgaaggacag aaaggtaccg
      421 gtagaaattc tctcccttat cccggaacct atcgctcgta cgcacaacat catcgggttc
      481 cgtaaagaga atggggaact ggaagtagca atgcttgata ccgaagacct tccggctatc
      541 gactctgtgc gtaagaagac gggacttaag attcttccgc gcctcaccga cgaagaatcc
      601 atcaagcacg cgctcctcca gtaccagaag tcattgaagg aagagttcgg cgacatcatc
      661 tcgaacgaag cagggaagat aaacctaatt aaggacgctg gcatcggtgg ggaagagatg
      721 agcggcgatg agctcaaaaa gatggcagaa gatctgccta tcgttcgcat cgtcgacacc
      781 ctcctgcgcc acgctattac tcaggacgcg tccgacattc acatagagcc aatggagatg
      841 gaggtgctcg tgcggtatcg catcgacggc attttgcatg acgcaatgac gctcccgcgc
      901 gcggctgctc ccggcatcac ggcacgcatc aaagtgctct cgaacctcaa gctcgatgag
      961 aagcgtctcc cacaggatgg acgcttcaag atggagaccg aagcggacaa ggtatcgttc
     1021 cgtgtttcac ttctccctac gtactacgga gagaaagtcg ttatgcgtct tctccgagag
     1081 actggcgaag gcttcacgct tgaagcactt gggttccatg gtgaaccgct cgaacgcctc
     1141 cataaagcaa cgaaagcaac caccggcatc atcctcgtgt cgggacctac gggttcgggt
     1201 aagacgacga ccttgtatac cattctcgat attttgaaca cgcccgacgt aaacatctct
     1261 accgttgaag atcctatcga gtatcagatg aagcgcgtga accagacgca ggtgaatcca
     1321 accatcggtt tctcctttgc aaacggcctg cgtgcgctcc ttcgccagga cccgaacatc
     1381 atcatggtgg gagaaatccg cgatgaagaa acggcctctc ttgcgattaa cgccgcactc
     1441 accggtcact tggtgctctc taccattcac accaactctg ctacgggcgc cattccgcgt
     1501 cttcttgata tgggcgtaga accattcctc cttgtttcaa cgcttcgtgt ggtcgtgggt
     1561 cagcgcctcg tgcgccgcct cgcggacgga aaggaacagt acacgcttac taaagctgaa
     1621 cgcgagaagg tggcgtcaga ggatcatttt gatgcagtga tgaaagcact cgcggaagaa
     1681 aagatagtga aagcaggaac aaagattgat gccgtgccct tctatcgtcc aaaggcagga
     1741 ggagagacag gcgacggata caagagtcgt atcggtatac acgaagtact tcaggtgact
     1801 ccatcgttgc gcgaaatgat tatgaagagc gctacctcag atcagctcga agagcaggca
     1861 cgcaaagaag gaatgctcac gatgcttgaa gacggtatgt ataaggcatc acgcggcatt
     1921 acgagtcttg aggaggtgct tcgcgcgatt acggaataat ttgtttttga tacaagaagt
     1981 ggcatagtgc gagcggagtt cttatatagt taaggatttc gctcaactca aacagaagga
     2041 tctcttcctg atgtctaaat tgaccagcgc cgtactcttc ggattcgcag tagcagtcgt
     2101 taccttcttc ttcagcgact cgctcatcat gtggtctggc ggtaaggcgc acgaaaacat
     2161 cgtgcttacg actgttatct acggctttct tgccatgctt tcagtctatt ggccaaaatc
     2221 aagcgccgtt ctcgcattct tcctgatttt tttaacgggc ctgctggtag tagtgctcgg
     2281 tgaaagtcag tcaggacgtg tagaaaaggt atttgtcgtg atacttcacg cgctgccatt
     2341 cttcctgccg ctcctatggt caactggcta ttttgatctt ttccgaaatg ctaaacgtac
     2401 gtcggcgtaa ccgacgacca ccccgtgcgc ttctcgatgt gggtcgagaa gtgcgcgggg
     2461 ttcgttttta acatgctacg attatcacta gcatgaagtt cacggtaacg attaagaaag
     2521 atgatggcgc agaagagaag cgcgttgtag aagcgccaac gcgctttgcg gtgtatgccg
     2581 atatggaaaa ggaaggggca accgtactcg ctctttctga gggaggtggg ggattcacga
     2641 tgcctgcgtg ggcaaatata cagctcacga gtggcgtgaa gacggaagag cgcatcacgt
     2701 tcaccaaaaa cctctcggca atgcttggtg ccggcctcac actctcacgc gcgctctccg
     2761 ttattgaccg tcagtccgca aacaaaggat taaagcgcat cgttgctgac ctcgaagcga
     2821 gagtgaagaa gggagacgcg ttcaacgaag cgctcgcatc gcacccaaaa gtattctccc
     2881 cacttttcat cgctatgacc aaggctggag aagagtcagg aactcttgct gatgcgttga
     2941 aggtagtcgc caagcagatg gaccgctctt ttacgcttca gaagaagata aagggcgcga
     3001 tgatttatcc tgccatcatt ctctttgcta tcgctatcat cggcgtgctc atgatggtat
     3061 atgtggtgcc tactctggca gcaaccttta aggagcttgg tgcgcagctt cctgcagcaa
     3121 ccttggccat catgtctatc tcagaattca tgtcgtcgca tgtcatcctg gtgttcgtca
     3181 tcctttttgc ctccatattt gccgtcatcg cgtttcttaa gacaaagatc ggcggaaata
     3241 tcgtgctcgc cggtgcactc catatgccgg ttattggtac tttggtgcgt gagacgtata
     3301 gcgcacgcgc agcacgcgcg atgtcatcgc ttctctcgtc aggcgtagaa atgctttctg
     3361 ctatttctat aacagaagag attgtaggtg ataaccgatt cgggaaagta gtagcagagg
     3421 cgtccgcacg cgttaaaaag ggagatgcgc tctccgctcc tttcatagaa catccgaaac
     3481 tttatcctgt gttcttcggt gaaatgattg cggtggggga ggagactggt caggttgccg
     3541 gcatgctcgc gcaggtggct gagtactatg agaatgatgt agaggaaggc accaaagacc
     3601 tctcgactat catcgagcca ctcctcatgc tcttcattgg tgcgttcgta ggtgtgttcg
     3661 cggtctcgat gattgctcct atctactccc tctcgtcgca aatctaaacc gacgctcggt
     3721 tgcgtgcgtt atcatagtct catgcgttct ttttccatgc gcggcatgtc gctcatcgac
     3781 gttatcgtcg gcgtagcgct tgtcgttact attttcgccg gactcttcg
//
LOCUS       DAORIN010000060         3811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_5255_length_3811_cov_5.414803, whole genome shotgun sequence.
ACCESSION   DAORIN010000060 DAORIN010000000
VERSION     DAORIN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5255_length_3811_cov_5.414803"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="PK109_02755"
     CDS             complement(<1..1533)
                     /locus_tag="PK109_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRH24482.1"
                     /translation="MRGALRLKLRIFSGAIIFFALLVIVRLYFVQIVDGEKYALKAEH
                     QYQNTSQSVYDRGSIYFTRKDGTLISAAGLGTGFRLAIDPQHLKDPEAAYAAVNALTP
                     VDYEEFMESAAKKDDPYESITTHVPEAAGRAIDELDIPGVLVERERWRSYPAGENGAQ
                     TIGFVAYDNDNTLAGRFGLERYYNDVLDRGKEGLFGNFFAELFANVGQAVADGRSGQS
                     GDVVTSIEPVVEEKLMQVLADIQAQYGSTETSGIIMDPATGEIIALGTYPTFDPNDFA
                     NGNADHFGNPLVERRYEFGSIMKALTMVSGLDAGVITPESTYNDTGKLTLNGKTISNF
                     DGKARGVVPMQQVLSQSLNVGAAHIANQMGHDKFRAYIKKLGLGTETGIDLPSEIPGQ
                     IDNILDSPRDIEYATASYGQGIAETPVEMIRALGALANRGSIVTPHLATAIRLETGLE
                     KKLSWGTPERVFKPEAVEQTTQMLVSVVDKSLANGSVRIPEMSVAAKTGTAQIAGPGG
                     KYA"
     gene            complement(1708..2004)
                     /locus_tag="PK109_02760"
     CDS             complement(1708..2004)
                     /locus_tag="PK109_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24483.1"
                     /translation="MPHKTIPILGFSCALLFAGYIVLVIATVFFATWETELASSASLT
                     ESRIAALETEYFDAIATLSETNVASAGYNHPERVEYVAANGKPTFTLAPTPATR"
     gene            complement(1994..2935)
                     /gene="rsmH"
                     /locus_tag="PK109_02765"
     CDS             complement(1994..2935)
                     /gene="rsmH"
                     /locus_tag="PK109_02765"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRH24484.1"
                     /translation="MKSLHESVLLKEAIDGLNIVPGDIVVDATVGGAGHFSAILDKLD
                     GNGALIGIDADASAIERGEAAAQHARAAVHLVEDNFRNLSSILDTLNIRQVSKVLFDL
                     GWSAYHLESGRGFSFRAEEPLLMTYGTPVHGQTAADMINSASEEALADLFFTLGEERF
                     ARQIAKGIVKERAHNRILTTGDLVKVILASTPAWYQHRRLHPATKTFQALRIAVNDEL
                     GALRQGLSSAIERLTPGGRIAVITFHSIEDRIVKTMLRDAAHAGVGTLVTKKPIAPSL
                     TETRENPRARSAKLRIFERGGAESESLSALSSYAYAS"
     gene            complement(2941..3396)
                     /gene="mraZ"
                     /locus_tag="PK109_02770"
     CDS             complement(2941..3396)
                     /gene="mraZ"
                     /locus_tag="PK109_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRH24485.1"
                     /translation="MNSPEPAHNTIMFIGEYRHTFDAKNRISLPAKFRKELGASVVVT
                     RGLDKCLFIYPKSAWKQEAAKIAAHSTGGAAGRGLARLMLAGASEADVDSAGRILVPD
                     YLKSFAGLKIKSVIAGVSDRVEVWEEDAWQNYTTSIERDADLFAEKLAA"
BASE COUNT          908 a          998 c         1037 g          868 t
ORIGIN      
        1 agcgtattta ccgccagggc cggcaatctg agcggtaccc gtctttgcgg ctaccgacat
       61 ctcgggaata cggaccgatc cgtttgcgag tgatttatcg actacggaca cgagcatttg
      121 cgtggtctgt tctactgcct ccggcttaaa cacgcgctca ggagtccccc acgacaattt
      181 cttctcaagt cccgtttcaa gacgtatcgc ggtagcgagg tgcggcgtga caattgagcc
      241 gcggttagcg agcgcgccaa gcgcacgaat catttcaacg ggggtctcag cgataccttg
      301 gccataggag gcagtggcgt actcgatatc acgcggacta tcaagaatgt tatctatctg
      361 tccgggaatc tcactaggta aatcgatgcc ggtctcagta ccaagaccga gctttttgat
      421 atacgcacgg aatttatcat gtcccatttg atttgctata tgagcagctc ctacgttaag
      481 ggattgtgaa aggacttgct gcatgggtac gacgccacgc gctttgccgt cgaagttgga
      541 aatggtcttt ccattcaagg tgagctttcc ggtgtcgtta taggtgctct caggggtaat
      601 cacgcccgca tcaaggcccg agaccatggt gagggctttc ataatggagc cgaactcgta
      661 gcgacgttcc accaaggggt taccaaagtg gtccgcgttc ccgttagcaa aatcatttgg
      721 gtcaaaggtg ggatacgtac caagcgcgat gatttctcct gtcgcagggt ccatgatgat
      781 gccgctcgtc tccgtgcttc catactgcgc ctgaatatca gcgagtacct gcatcagttt
      841 ctcttcaacc accggttcaa tagaggtgac cacatcgccc gactgtccgc ttctcccatc
      901 ggcaactgcc tgcccaacgt ttgcgaaaag ttctgcgaag aaattgccga agagtccctc
      961 tttccctcta tcgagtacgt cgttgtagta acgttcaaga ccaaagcgtc ctgcaagcgt
     1021 gttgtcgtta tcataggcca caaacccaat ggtttgtgca ccattctctc ctgctggata
     1081 actgcgccaa cgctcacgct caactaacac gccagggata tcaagctcat cgatagcgcg
     1141 cccggcagct tctgggacgt gggtcgttat tgattcatag ggatcatcct tctttgcggc
     1201 tgattccatg aattcttcgt agtcaacggg agtcagcgca ttcactgcag cgtatgccgc
     1261 ctcaggatct ttcaggtgct gcggatcgat agcaaggcgg aagcccgtac caagcccggc
     1321 agctgagata agcgtgccgt ccttgcgggt gaagtatatg gaaccacggt catagactga
     1381 ctgactcgtg ttctggtact gatgttctgc cttaagggcg tatttctccc catccactat
     1441 ctgtacgaaa tacagccgca cgataacgag tagtgcaaaa aagataatcg cgcccgagaa
     1501 tatgcggagt ttaagccgaa gggcgccgcg catggttagg aggcgtagac gaacccttct
     1561 tcctgtatct cgtgctttgc ttcaatcaac aaacgtactg agggtacaaa ccctactgcg
     1621 acacgctttt tcccaacgca ggtcacacca cagcagcggg agcaggaagc gaaccgagca
     1681 agatagtttc gtgttctatg gcgcatatca gcgggtggca ggggttggcg caagcgtgaa
     1741 ggtcggcttg ccgtttgcgg caacgtactc tacgcgctct gggtggttat accctgccga
     1801 ggcaacgttg gtttcgctca aggtagcaat ggcatcaaag tattcggtct caagggctgc
     1861 gatgcgtgat tcggtaaggc ttgcggagga cgcaagctcc gtctcccagg ttgcaaagaa
     1921 gacggtagca ataacgagta cgatgtagcc cgcaaaaagg agcgcgcagg agaacccgag
     1981 gatgggaata gttttatgag gcatacgcgt aggaagaaag agcggacaat gattctgatt
     2041 cagcaccacc gcgctcgaag atgcgcagct ttgcgcttcg agcacgagga ttctcacgcg
     2101 tttcggtaag tgacggcgct attggttttt tagtgaccaa cgtccccact cctgcgtggg
     2161 ccgcgtcgcg aagcatggtc ttaacgatgc gatcctcgat gctgtggaag gtgatgacgg
     2221 caatgcgacc tccgggagta aggcgctcga tagcgctaga aagaccctgg cggagcgcgc
     2281 cgagctcatc attcaccgca atgcgcagtg cctggaaggt tttcgttgct ggatgaagac
     2341 ggcgatgctg ataccacgca ggcgttgacg caagaataac ttttacgagg tcacccgtgg
     2401 tgagaatgcg gttgtgcgca cgctctttga cgatgccttt ggcaatctgg cgcgcaaagc
     2461 gctcttcacc gagcgtgaag aataggtcag cgagcgcttc ttcagacgct gagttaatca
     2521 tgtcagcagc agtctgtccg tgtactggcg tgccgtacgt cataaggagg ggttcctctg
     2581 cgcggaagga gaagccgcga ccgctctcaa ggtggtaggc gctccagcca agatcaaaga
     2641 gcactttgga tacctgacga atgttaagag tatcgagtat agaagaaagg ttgcggaagt
     2701 tatcttccac gagatgcacg gcagcgcgcg catgctgcgc agcggcttcc ccgcgttcaa
     2761 tcgctgatgc atcagcgtca atgccgataa gagcaccgtt accgtcgagc ttatcgagga
     2821 tggcactgaa gtgccctgcc ccacctacgg tagcgtcaac gacaatatct cccggaacaa
     2881 tattcagtcc gtcgatggct tctttcaaaa ggacgctttc gtggagggat ttcatacagg
     2941 ttatgcggca agcttctctg caaagaggtc agcatcacgc tcaatagagg tggtgtagtt
     3001 ctgccacgca tcctcttccc aaacctcaac gcgatcagaa acgccagcga tgacgctctt
     3061 tatctttaat ccggcaaacg atttcaaata atcaggaacc aaaatgcggc ctgcgctatc
     3121 aacatcagct tctgaggcgc ccgcaagcat aaggcgagca aggccacgac cggctgctcc
     3181 tcccgttgaa tgtgctgcaa tctttgctgc ttcctgtttc cacgcactct tcgggtatat
     3241 aaaaaggcac ttatcgagtc cgcgagtcac tacaaccgac gcgcccaatt ctttccggaa
     3301 ctttgccgga agtgaaatgc gattcttcgc gtcgaatgtg tggcggtact ctcctatgaa
     3361 catgattgtg ttatgagcag gctctgggga gttcatgaac caaatttcat gaatcccact
     3421 tcatcccact agtaggtaag tatatgacac tacgtcccac ttccctccct aaacctgtgt
     3481 ggataagggg gataaccgga gttgacccgg cacctgagag gtataggcta caactacaca
     3541 agcgcgtcgg ggtctgactc cagcatttac cccctgaaaa ttggagccgg ttcctgaccg
     3601 ttacggtcag gaaccggctt gcattaggct gcgaggcaca ttcgggacaa gtaaccgcct
     3661 aaggttcagt ccagtgagga catcggtaga tcagccaaca gaagaagaaa agctcttaaa
     3721 cgagcagtga gtgcagcaag acaggaaaga agacagaaac gagagaatgg tacgcaagat
     3781 cgcgtgaatg gtaggttcgg catcccgagc a
//
LOCUS       DAORIN010000061         3734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_5459_length_3734_cov_4.740147, whole genome shotgun sequence.
ACCESSION   DAORIN010000061 DAORIN010000000
VERSION     DAORIN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5459_length_3734_cov_4.740147"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PK109_02775"
     CDS             <1..380
                     /locus_tag="PK109_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24486.1"
                     /translation="AINERIIADIALAAAEAMGSNERYTPDKLSCRSDGSPVSVWGDS
                     ADMRDALGWILKKSCDHEKIADIGVRQVRNAAYQVSELLNEQVIALTGRSLVTISSDD
                     GREAVVKVLSQIAENIQAKYRTA"
     gene            455..811
                     /locus_tag="PK109_02780"
     CDS             455..811
                     /locus_tag="PK109_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24487.1"
                     /translation="MKDTAMYRATLEEEKGRLEKELGSIGRRNPANPADWEAVPGETG
                     LEADSTDTAEQIDGYETNTAILKELETRYNHVLAALSRIEGGTYGVCAVGGEAIEAER
                     LGADPAATTCKAHLNG"
     gene            822..1460
                     /locus_tag="PK109_02785"
     CDS             822..1460
                     /locus_tag="PK109_02785"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HRH24488.1"
                     /translation="MTSTLSVKECLSFGWRTFKARPWFFVGTFLIYAVIQGIMSSIQQ
                     NMPGLVSFLLSLIVSTLLYIGLITIYLKAHADAKAPQYADFWHPGTFWKYLGASLLLA
                     VIVIVGLILLIVPGIIAALALSFTGYLVVDKGMNPIAALKESARLTKGNRWKLFLLGL
                     AIFGLSILGMIPLFLGLLIVGPVSMLAGVHAYRTLEHPASVAPVAVETPVAA"
     gene            1467..2054
                     /locus_tag="PK109_02790"
     CDS             1467..2054
                     /locus_tag="PK109_02790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRH24489.1"
                     /translation="MGLLITPSESEKTTLPSQLYESTVLATAIADDGSAFTIVAGLTE
                     AIAADLKRHSLDESDVELMKSTSDKRRFGEGSFETWFKKERYAFALVDGDALAALIWF
                     GPEEMPHEFQSEHYVLGTHDTLGFRSYEPYRGKRLMTDFSRFVIDAYARMRPGRTLWL
                     QTNTDNGAAIRLYQKLGFIEKGVGTDNGRLVMVQP"
     gene            2056..3207
                     /locus_tag="PK109_02795"
     CDS             2056..3207
                     /locus_tag="PK109_02795"
                     /inference="COORDINATES: protein motif:HMM:NF027440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HRH24490.1"
                     /translation="MPRVKLTEYRAKKILLGDSYRGAAATTENTPSLPEGKYVVKVDQ
                     GIKKRMKRGLVALNKTAHEVGSPFKDWSAKGYTNFIIEPMLPHEGSEERYISFERVRD
                     GIRVLYAAEGGIEIEEHPEKVTTWILRTKEEVATVAQTTGIPVSFFESVMRAFDEEYF
                     AFLEINPLVVQNDTAYLLDAAALVDSAAQFFARGWGAGDVVKTLAKHPAEEAVEALAA
                     TTPASLKLNIISSNGSLFFLLSGGGGSIVIADEAELRGMGKEIANYGEYSGGPTREET
                     YLYTKEVLGVVLASSASKKALVIAGGVANFTDVKTTFGGIIDALSEVAGELQKAGVKV
                     FIRRGGPNEAEGLALMRAFLEKQNILGTIYGSDAIITSAVDDAIDYLKS"
     gene            3204..>3734
                     /locus_tag="PK109_02800"
     CDS             3204..>3734
                     /locus_tag="PK109_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24491.1"
                     /translation="MNFIEKIRNGEDSIVVMGNHPRIVQSVVDFDYLSGKKAPSVLAI
                     ITGNKKAHKCFWGKKEILIPCYAKATQVPEDVARRARWMLNVQSGRRAYESTVSFFET
                     FPEALGGTIFAENVPEKHATEMIARYGATKLLSGPASVGILVPRALKLGGIGGIDPKQ
                     IEEGRLTSEGSIAVVST"
BASE COUNT          925 a          958 c          993 g          858 t
ORIGIN      
        1 ctgctatcaa cgagcgtatc atcgctgata ttgcactcgc cgcagctgaa gccatgggga
       61 gcaatgagcg atatacccct gataagctct cctgccgctc agatggcagc cctgttagcg
      121 tttggggaga tagtgccgat atgcgagatg ccctcggttg gattctaaaa aagtcgtgtg
      181 atcatgaaaa aatcgccgac atcggggtac gacaagtgcg caacgctgcc taccaagtat
      241 ctgagctgct gaatgagcag gtgatagctc ttactggtcg ctcattagtg actattagtt
      301 cggatgatgg tcgcgaagca gtcgtaaagg tcttgagcca gattgctgaa aacatccaag
      361 caaaataccg aactgcttaa acttaaagcc cgcgccttac cgcgcgggct ccttattggt
      421 agagatcgtg gcacaatatg ctagactggc gcgcatgaag gacactgcga tgtatagagc
      481 gacccttgaa gaagaaaagg gacgactcga aaaggaactt gggagtattg gccgccgtaa
      541 ccccgccaat cccgctgatt gggaagccgt tcccggagaa accggccttg aggcggattc
      601 cacggatacc gctgaacaga tagatggata tgagacaaat accgctatac ttaaggaact
      661 cgaaacccgc tataaccatg tgcttgcggc gctttcgcga atagaaggag gaacctatgg
      721 cgtatgcgcc gtcggcggag aagcgataga ggcagaacgc cttggagcag atcccgcagc
      781 aaccacgtgc aaagctcacc ttaatggcta actaactaat catgacttca accctttcag
      841 taaaagaatg cctttcgttc ggctggcgca cctttaaggc ccgcccgtgg ttctttgtgg
      901 gcaccttcct tatatacgcc gtcatccagg gcatcatgtc gtccattcag cagaacatgc
      961 caggactcgt aagcttcctc ctctctctta tcgtctccac gctcctctac atcggcctca
     1021 tcactattta ccttaaggcc catgctgatg cgaaggctcc acagtacgct gacttctggc
     1081 acccagggac gttctggaag tacctcggtg cttctctctt gctcgccgta atcgtgatcg
     1141 tcggtctcat cctcctcatc gtgccgggca tcatcgctgc gctcgcactc tccttcaccg
     1201 gctacctcgt cgttgataaa ggcatgaacc cgatagcagc acttaaagag agcgcacgcc
     1261 tcacgaaggg taaccgttgg aagctcttcc tgctcggtct tgccatcttc ggcctctcta
     1321 tcctcggtat gattcctctt ttcctcggtc ttctcatcgt tggcccggtc tccatgcttg
     1381 ctggcgttca cgcataccgc acgcttgagc accctgcctc ggtagcaccc gtcgcagttg
     1441 aaactccagt agctgcctaa ggaggcatgg gtcttctcat caccccctca gagtctgaga
     1501 agacgacgct tccttcgcag ctgtacgaaa gtaccgtact cgctactgcc atcgctgacg
     1561 atggcagtgc gtttactata gttgcaggac tcacggaagc gatagcagct gacctgaagc
     1621 gacactcgct tgatgagagc gacgtggagt tgatgaagag cacgtctgat aaacgccgct
     1681 tcggtgaagg atcgtttgag acgtggttta agaaagagcg gtatgcgttc gcgctcgtcg
     1741 atggtgacgc acttgccgcc cttatatggt ttggtcctga ggagatgcct cacgagtttc
     1801 aatccgaaca ctacgtactc ggcacccatg acacgctggg gttccgttcg tatgagccgt
     1861 atcgcggcaa acgactcatg actgacttct ctcgcttcgt cattgacgcg tatgcgcgta
     1921 tgcgtccggg acgcaccttg tggctccaaa caaataccga taacggagca gcgatacgcc
     1981 tctatcaaaa gcttgggttc atagaaaagg gagtgggtac tgacaacggg aggcttgtga
     2041 tggtacaacc gtaatatgcc gcgcgtaaaa cttaccgaat atcgtgcaaa gaaaatactg
     2101 ctcggtgact cgtatagagg agcggcagca actaccgaaa acacgccttc gcttccggaa
     2161 gggaagtacg tagtgaaagt agatcaagga ataaaaaagc gcatgaagcg tggactcgtc
     2221 gcgttgaata aaaccgcgca cgaagtgggg agtccattca aagactggag cgctaaggga
     2281 tacaccaact tcatcatcga accgatgctg ccgcatgagg gaagtgaaga gcgttacatt
     2341 tctttcgagc gtgtgcgtga cggtattcgt gtgctgtatg ccgcagaagg tggtattgaa
     2401 attgaagagc acccagaaaa agtaacaacg tggatactac gcaccaaaga agaggtggca
     2461 acagtagctc aaacgacggg cattcctgtg tcgttttttg aatcagtgat gcgcgcattt
     2521 gatgaggaat attttgcctt ccttgaaatc aacccgctcg tcgtacaaaa cgataccgcg
     2581 tatcttttgg acgctgccgc gctcgttgat agtgctgcgc aattctttgc gcgcggatgg
     2641 ggagcgggag atgtggtgaa aacgctcgcc aaacatcctg cagaagaggc ggtggaagca
     2701 cttgctgcaa cgacccctgc atcattgaag ttgaatatta tttcttccaa cggatcttta
     2761 ttcttccttc tttcaggggg aggtggttct atcgtcatcg ccgacgaagc agagttacgc
     2821 ggcatgggaa aagaaattgc taactatggc gaatacagcg gcggcccaac acgtgaagaa
     2881 acgtatctct acaccaaaga agtactcggc gttgtactcg cttcgtcggc atccaaaaaa
     2941 gcacttgtca ttgccggggg cgttgcgaac ttcactgacg tcaaaactac gttcggcggc
     3001 atcatcgatg cactctccga agtagcgggg gagttacaga aggcaggagt gaaagtattc
     3061 attcgtcgtg gcggcccgaa cgaagcagag ggacttgcgc ttatgcgtgc atttcttgag
     3121 aagcaaaaca ttcttggcac catctacggt tccgacgcga ttattacgag cgcggtggac
     3181 gacgctatcg actatttgaa atcatgaact tcatagaaaa aatcaggaat ggggaagaca
     3241 gtattgtcgt gatgggaaac catccgcgta tcgtgcagtc ggtagtggac ttcgactatc
     3301 tttccggaaa aaaggcaccg agtgtgctcg ctatcatcac ggggaacaaa aaagcgcata
     3361 agtgtttttg ggggaagaaa gagattctca ttccgtgcta tgcgaaagcg acgcaggtac
     3421 cagaagacgt tgcacgccgc gcacgctgga tgcttaacgt acagagcgga cgccgtgcgt
     3481 acgaatctac cgtgtcattt ttcgaaacat tccccgaagc actcggcggc accatctttg
     3541 ctgagaacgt tccggagaag catgccactg aaatgattgc gcgctacggt gccacgaaac
     3601 ttctttcagg acccgcgtcg gttggcatac ttgttccacg cgcattgaag ctcggcggta
     3661 tcggtggtat tgatcctaag cagattgaag aaggacgtct caccagcgaa ggttccatcg
     3721 ctgtggtgtc tacc
//
LOCUS       DAORIN010000062         3710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_5525_length_3710_cov_4.526949, whole genome shotgun sequence.
ACCESSION   DAORIN010000062 DAORIN010000000
VERSION     DAORIN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5525_length_3710_cov_4.526949"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PK109_02805"
     CDS             <1..672
                     /locus_tag="PK109_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24492.1"
                     /translation="LTKLLTLYAAADALGADAPVTITARALATEGESGFSEGDTFTFE
                     DAARMALVASSNDAAEAIAEAATTVEHAPNKTSLLASAAAAIGLTQTYALNGTGLDEN
                     GEISGGYGSAHDIALLAAALLKRVPGIMHASIDPSVTVKSLAGSSYTLKNTNPEVSRI
                     PGLMLSKTGYTDLAGGNLAVVFDAGIGHPVAIVVLGSTRDERFTDVDRLVKATGEQFA
                     GLSLP"
     gene            669..1730
                     /locus_tag="PK109_02810"
     CDS             669..1730
                     /locus_tag="PK109_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24493.1"
                     /translation="MILDAARVLIPFVVSFTFGMILAPVLSHYLYKHKAWKKKAGKGV
                     GMGDDAGTPLFDELHAVRETSAPRMGGIIVWGSVVLTVLLFAILSYFYGGAWVDLDFV
                     NRGQTWLPLFALIAGALVGLIDDILEVTKSEGGLALPWRLILVTALGTFAGWWFYDKL
                     DISAVGIPFVGALPLGVFFIPFFVFVTLAIYASGVIDGIDGLAGGIFAIVFMAYAGIA
                     FAQEQLALSAFAAAVSGGLFAFLWFNIPPARFYLSETGTMALTLALAVTAFSADVLGD
                     GVGVAVLPIIAFPLTATVFTSVLQVVWKKVFKKKLFRIAPIHHHFEAIGWPSYKVTMR
                     YWIITIIASIFGLAVALLK"
     gene            1780..2895
                     /locus_tag="PK109_02815"
     CDS             1780..2895
                     /locus_tag="PK109_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRH24494.1"
                     /translation="MRAISGDRVFFFIVVILVVGGIAMFASASLGLLAREGDSPWKLA
                     ITQLVLGLIPGIAALLAIRYAPPTWIQKLAPIVYIGSIGLTALVFIPGIGGTYNGATR
                     WIDLGFTTVQPAEFLKIGVILMLAWYLSKMKGKLDDIRTGLIPFGLIVGIPSVMLLLQ
                     PNTSTVMVMGLTAVALYLLAGAPWRDILLIIAGAVLVLAVLVMQRPYLMSRVATFMNP
                     ADNSLTSGYQIQQSLIAIGSGGFMGRGFGQSAQKFNYLPEPVGDSVFAVYSEEFGFVG
                     AAFMVLLFAGFAMRGFAIAADAATVFGSLAATGLTLLISLSAFLNIGAMLAILPLTGL
                     PLPFVSHGGTALLVALASVGVILNVAAHRKQGRKANA"
     gene            2917..>3710
                     /locus_tag="PK109_02820"
     CDS             2917..>3710
                     /locus_tag="PK109_02820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRH24495.1"
                     /translation="MKIVFTGGGSGGHFYPLIAVAESVRAIARERRLVSPKLFYIAPS
                     AFDQEALFENEIVFVGCPAGRWRRYFSLQNFTDLFVTASGLVSALFTLFSIYPDVVFS
                     KGGYASVPVVLAARILGIPVIIHESDAKPGRANIMASKFAYRIAVAFDSAIAFFPAKV
                     RDRVARTGIPIRAAIAMPESQGAAQELGLDPAVPTLLILGGSSGARAINDVVVSALSE
                     LVESANIIHQTGKNNIAGVESLAKLVLEKSPYAVRYHPFAYLNTLS"
BASE COUNT          722 a          988 c         1013 g          987 t
ORIGIN      
        1 ctcacgaagc ttcttactct ctacgctgcc gctgacgcgc ttggtgctga cgcaccggtc
       61 accattacgg cacgtgcgct cgcaaccgaa ggcgagagcg gattctcaga aggtgacacc
      121 ttcacctttg aagacgctgc gcgcatggca ctcgtcgcaa gttcaaatga tgctgcagag
      181 gctatcgctg aagcagctac cacggttgaa cacgctccga ataagacgtc acttcttgca
      241 agcgcagcgg cagccattgg ccttacccaa acctatgcct taaacggtac gggacttgat
      301 gagaacggcg agatttctgg cggctatggt tcggcacatg atattgcgct tcttgccgca
      361 gcactcctaa agcgtgtacc gggcatcatg cacgcgtcta ttgatccaag cgtgacggta
      421 aagtcactcg ctggctcttc ctacacgctt aagaacacga acccggaagt ctcccgcatc
      481 cctggcctca tgctctcaaa aacggggtac acggatcttg cgggcggtaa ccttgcggtg
      541 gtgtttgatg ccggtatagg gcatccggta gcaattgtag tgcttggctc cacccgtgat
      601 gaacgcttca cggatgtaga ccgtctcgta aaagcgacag gagagcagtt tgccggtctt
      661 tctcttccat gattcttgac gcggcacgag tactcattcc gtttgtcgtt tcgttcacgt
      721 tcggcatgat tcttgcacct gttctttcgc actatttgta taaacataag gcgtggaaga
      781 aaaaggcagg gaaaggggtt ggtatgggag acgatgccgg cacgccactg tttgatgaac
      841 tccatgcagt acgcgagacg tcggcaccgc ggatgggcgg cattatcgtt tggggctcag
      901 tagtactgac cgtccttctt tttgcaattc tctcgtactt ctatggcgga gcgtgggtag
      961 atcttgattt cgtgaatcgt ggacagacct ggctcccact ttttgcactt atcgcgggcg
     1021 ctctcgtcgg tcttatcgac gacatcctgg aagtaaccaa gagtgagggc ggactagcac
     1081 tgccgtggcg cctcattctc gtcactgcgc ttggtacctt tgccggctgg tggttctacg
     1141 acaaacttga tattagcgct gtagggattc cgtttgtggg agcgttaccg ctcggagtat
     1201 ttttcatacc attcttcgtg ttcgtgaccc tggctatcta cgcctcgggc gtcatcgatg
     1261 gcatcgatgg acttgccggt ggcatcttcg ccatcgtctt catggcgtat gcgggaattg
     1321 cgtttgcaca ggagcagctc gcgctttcgg cgtttgcagc ggcagtctca ggaggactct
     1381 ttgccttcct ttggttcaac atccctcctg cacgcttcta tctttctgaa acggggacca
     1441 tggcacttac gcttgcgctt gcggtcaccg cattctccgc agacgtgctc ggtgacggag
     1501 taggtgttgc ggttcttcct atcattgcgt tcccgcttac tgctacggtg ttcacgagcg
     1561 ttctccaggt ggtatggaaa aaggtgttca agaagaagct cttccgtatt gctcctattc
     1621 accatcactt tgaagcgata gggtggccct catacaaggt gacgatgcgc tactggatca
     1681 tcaccatcat cgcttctatc ttcggtcttg ccgttgcact ccttaaataa acgatagggg
     1741 atagagtaga agtcacgctg cggtactata ggaatagtca tgcgcgctat atctggagat
     1801 agagtgttct tctttatcgt cgttatcctc gtggtaggcg gtatcgccat gtttgcgtcc
     1861 gcgtcccttg gtcttcttgc gcgtgaagga gactcaccct ggaagctcgc catcacccag
     1921 ctcgtccttg gactcatacc gggcattgca gcgcttctcg ctattcgata cgcacccccg
     1981 acctggattc aaaagcttgc accgattgtc tatatcggaa gcataggatt aaccgcgctc
     2041 gtgtttatcc cgggcattgg tgggacgtat aacggagcga cccgctggat agatttgggg
     2101 ttcacaacgg tgcagccagc agagttctta aagatagggg tgatactcat gcttgcttgg
     2161 tatctctcca agatgaaagg gaagctcgat gatatacgca cggggctcat accgttcggg
     2221 cttatcgtcg gcattccttc agtgatgctc ctcttacagc cgaacacctc taccgtgatg
     2281 gtgatgggac tcacggcagt agcgctctac ttacttgcgg gagccccctg gcgcgacatc
     2341 ctcctcatta tcgcgggtgc ggtattagtg ttggcggttc ttgtgatgca gcgcccctac
     2401 cttatgagtc gtgtcgctac ttttatgaat ccggcggata actcactcac aagcgggtac
     2461 cagattcagc agtcattaat tgccataggg tcgggcgggt tcatgggacg cgggttcggg
     2521 caaagtgcgc agaagtttaa ctaccttccg gaaccggtag gggactcggt gttcgcggta
     2581 tatagcgaag agttcgggtt cgtgggtgcg gcgttcatgg tgcttctgtt tgctggcttt
     2641 gcgatgcgcg gctttgcgat agcggctgat gctgcaacgg tcttcgggtc cttagcggca
     2701 acgggtctca cgcttctcat atccctttca gcatttctca atattggagc gatgcttgcc
     2761 atccttccct taaccgggct tccgcttccg ttcgtgtcgc atggtggtac ggcgctctta
     2821 gtcgcgctcg cctcggttgg agttattttg aatgtggctg cgcatcgcaa acaggggagg
     2881 aaggccaacg cctaaaaagt gttataatcc gcgcaaatga agatcgtatt tacgggggga
     2941 ggcagcgggg gacatttcta cccccttatc gcagtagcag agtcagtacg cgctatcgcc
     3001 cgtgagcgtc gtttggtgtc tcctaagttg ttctatatcg ctccttccgc ctttgatcag
     3061 gaggcgctct ttgagaatga aatagtgttc gttggttgtc ctgcaggacg gtggcgccgc
     3121 tatttctccc ttcagaactt caccgacctc tttgtaacgg caagcggatt ggtatcggca
     3181 ctcttcaccc tcttctccat ctacccagat gtcgtgttct ccaaaggtgg ctacgcgagt
     3241 gttccagtgg tacttgccgc acgcatcctt ggcatcccgg tgattattca tgagtcggat
     3301 gcgaagccag gacgcgccaa tatcatggcc tcaaagtttg cctaccgaat tgcggtagcg
     3361 tttgacagcg ctatagcatt cttcccagca aaggtgcgtg atcgcgtcgc tcgtactggt
     3421 attcctattc gtgctgctat cgcaatgccg gagtcgcagg gtgccgcaca agaactgggt
     3481 cttgatcctg cggtgccgac gctccttatt ctcggcggct cctcaggtgc tcgtgccatt
     3541 aatgacgtgg tagtgtccgc gctttctgaa ctcgttgaga gcgccaacat catccatcaa
     3601 acaggaaaga acaatattgc tggcgtggaa agccttgcaa aacttgtgct tgagaagagt
     3661 ccgtatgcgg tgcgctatca cccgtttgcc tacctcaaca cgctttccat
//
LOCUS       DAORIN010000063         3698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_5554_length_3698_cov_5.193522, whole genome shotgun sequence.
ACCESSION   DAORIN010000063 DAORIN010000000
VERSION     DAORIN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5554_length_3698_cov_5.193522"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PK109_02825"
     CDS             <1..525
                     /locus_tag="PK109_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRH24496.1"
                     /translation="KAITALGRFPKTPEALEKLPGIGPYTARAVAAFAHNADVVFIET
                     NLRTVVTHHFFPRKKVVSDKELLAILEKAFPKGKSREWYAALMDYGAHLKRSGVRLNA
                     KAKGYKKQKVFRGSSREARGAILKALVRGPRDAEFLTHILGDDRVVQAKSQLASLTKE
                     GLIELSNGKFRLPQ"
     gene            complement(581..871)
                     /locus_tag="PK109_02830"
     CDS             complement(581..871)
                     /locus_tag="PK109_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24497.1"
                     /translation="MKHAPIVEHTHHRATKVTRSEDGLFYRFDLYCVKDSTLFNLLTG
                     VPMRQTSQNGYHAEFTLPAQVVAEMFAGVAEAAMTPADSDDAADRILAPATA"
     gene            complement(974..2923)
                     /locus_tag="PK109_02835"
     CDS             complement(974..2923)
                     /locus_tag="PK109_02835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRH24498.1"
                     /translation="MTPAPAPAPAPAGAPREPRRESDGGPRRSGGFDRRGPRGGGGRG
                     PGGPGGGPRRGGQGRGRGDRRPPGGGMNRSGGQAPTPKRVADYIPAPVDEKTVRIVPL
                     GGVEEVGRNMFAVETGNDIFVCDVGFQFVSEDTAPGVDYVLPNTKYLEVNADRVRAVF
                     ITHGHLDHIGGIPFLMTRFGNPPIYTCNLSAVMIAKRQEEYPDMPKPQLVVVEPGQTV
                     TIGSTRVKFFAVTHSIPDSMGISIETPWGNVVATGDLKLDHNNGIPTDEEQKKWGEIG
                     RDNNLMFIADSTNAEKDGFSIPERKVHETLEEIVKSVSGRLIIGTFASQFERMIHIIE
                     TAEKLGKKIVTEGRSIKTNLEIAQKTGLLTIKKETLIPAQEIGDYPPDKIVILATGAQ
                     GEEFAALMRIATKQHKNITLGARDTVVLSSSVIPGNEINVQKLKDNLYRNGVTLLHYR
                     SSDVHSTGHGNTGELVWINKQVNAKFFMPAYGYYSMTSCHAKAVEQAGRPRESIIIAD
                     NGNVIDLIEGKTMFVHKDKVPNAPLVVDGFAIGDMQEVVIRDRQALAKDGMFVIIATV
                     NLKTGKLRKSPDIISRGFVYLRESQELLNTARLLIKKTVEDSTHGMNPVDLDYVKNQL
                     TDTMAGYLFSRTNKAPMVIPVLIGV"
     gene            complement(2994..>3698)
                     /locus_tag="PK109_02840"
     CDS             complement(2994..>3698)
                     /locus_tag="PK109_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRH24499.1"
                     /translation="GGYGTPVWGPVPPGSGVTIAAPEVSEDSIGAASAILEKNGWEYN
                     EETKRWKHEDLELTVTLKTSNVPELKAIAEAIRSDWEKLGVPVAIEYYDPSDLAASVI
                     RPRAYDALLFGMVVGRDRDLFAFWDSSQRTDPGLNIALYASRAVDTLLESMRVEQDPA
                     KVDEDLAEVDALIAADYPAVFTHAPDFVYALPEDIHGVSLARITAPTDRLAGVSHWYR
                     RTQWVWPAFAPGSVRQ"
BASE COUNT          790 a          985 c         1041 g          882 t
ORIGIN      
        1 aaagctataa cggcgctcgg gcgattccct aagacgcctg aagcactcga aaagcttccg
       61 ggtattggtc cctacaccgc gcgagcggtt gccgccttcg ctcacaacgc cgatgttgta
      121 tttatagaaa cgaaccttcg tacggtggta acacaccatt tctttccgcg taagaaagta
      181 gttagtgata aagaacttct cgcgatactt gagaaagcct ttccgaaagg gaagagtcgg
      241 gaatggtatg ccgccctcat ggactatggt gcccatctaa agcgttccgg agttcgattg
      301 aatgcaaagg cgaaagggta taagaaacag aaagtatttc gtggctcttc tcgcgaagca
      361 cgcggcgcaa tattaaaagc gctcgtgcga ggaccgcgtg atgcagaatt cctaacacac
      421 atcttgggtg atgaccgtgt cgtacaagca aagagtcagc tcgcctcgct caccaaagaa
      481 ggactcattg aactttccaa cggaaagttt cggttgccgc aataaagaag aatggccctg
      541 caagtaatgt agggccattc atagtgtcgt gatgagccga ctaggctgtc gctggtgcga
      601 gaattctgtc ggcagcatcg tctgaatcag cgggcgtcat tgctgcttct gcgacgcccg
      661 cgaacatttc cgcaacgact tgcgcaggga gcgtgaattc ggcgtggtag ccgttctggc
      721 ttgtctgtcg catcgggacg ccggtcagca ggttaaagag cgtgctgtct ttcacgcaat
      781 aaagatcaaa gcggtaaaag agaccgtcct cagatcgtgt gactttcgtc gcgcggtggt
      841 gagtgtgctc tacgataggg gcgtgtttca tgtcgtaact ccttctctct gcccgtgacc
      901 cggatagtac gtgctaggta tataaatgca aaccgccacc ctctcgggtg gcggtttgtt
      961 gcaacctagc tacttagact ccaatgagca ctggaattac catcggtgcc ttgttcgttc
     1021 tcgagaagag gtagcccgcc atggtgtcgg tgagctggtt cttcacgtag tcgaggtcta
     1081 ccgggttcat tccatgggtt gaatcttcaa cggtcttctt aatgagaaga cgtgcggtgt
     1141 tcaagagctc ctggctctcg cgtagataca cgaagccacg gctgatgatg tctggagact
     1201 tccgcagctt tccggtctta agattcaccg ttgcaatgat gacgaacatg ccgtccttag
     1261 cgagtgcctg acggtcacga atgaccactt cctgcatgtc accgatagca aagccatcca
     1321 cgacaagcgg tgcattcggc accttgtcct tatggacgaa catcgttttt ccttcgatga
     1381 ggtcgatgac gttaccgttg tcagcgatga tgatgctctc acgcgggcgt cccgcctgct
     1441 ctaccgcttt tgcatggcac gaggtcattg agtagtagcc gtaggcaggc atgaagaact
     1501 ttgcgttcac ctgcttgttg atccatacga gctcacccgt gttgccgtga ccggtggagt
     1561 gcacgtccga agagcggtag tgaaggaggg taacgccgtt tcggtacagg ttgtccttca
     1621 acttctgcac gttgatttcg ttacctggaa taacggacga ggagagcacc acggtatcgc
     1681 gcgcgccgag cgtgatgttc ttatgctgct tggttgcaat gcgcataaga gcagcgaact
     1741 cttcgccctg agcacccgta gcaaggatga ctatcttgtc tggtgggtag tcaccgattt
     1801 cttgtgcggg gataagcgtc tccttcttaa tagtgagaag accagtcttc tgcgctattt
     1861 caaggttcgt cttaatagag cgaccctcag tcacaatctt cttgccgagc ttttcagccg
     1921 tctcgatgat gtgaatcatg cgctcgaact gagacgcgaa cgttccgatg atgagacgtc
     1981 cagacactga tttcacaatc tcctcaagtg tctcatgcac tttgcgctca gggatagaga
     2041 agccatcctt ctcggcgttg gtggagtcgg cgatgaacat aaggttgtta tcgcgtccta
     2101 tctcacccca cttcttctgc tcttcgtcag tagggatgcc gttgttgtgg tcgagcttca
     2161 agtctccggt agcaacaacg ttgccccaag gggtctcaat ggagataccc atcgagtcgg
     2221 ggatagagtg cgtcacggcg aagaacttca cgcgagtaga tccaatggtt accgtctgac
     2281 ctggctccac taccacgagc tgtggttttg gcatgtccgg gtactcttcc tggcgctttg
     2341 cgatcatcac cgcagaaagg ttgcaggtgt agataggggg gttcccgaag cgggtcatga
     2401 ggaagggaat accaccgatg tggtcgaggt gaccgtgcgt gatgaagacg gcgcgtacac
     2461 ggtctgcatt cacttcaagg tatttcgtgt tcggaagcac atagtcaaca ccgggagcgg
     2521 tgtcttcaga aacaaactgg aagccgacgt cacagacgaa gatgtcgtta ccggtctcaa
     2581 ccgcaaacat gttgcgtcct acttcttcaa cgccgccaag cgggacaata cgtactgtct
     2641 tttcatcaac gggggcgggg atatagtcgg caacgcgctt tggggtaggg gcctggccgc
     2701 cagagcggtt catgccgccg ccgggtgggc gtctgtcgcc gcgtccacgg ccctggccgc
     2761 cacgtcgtgg gccgcctccg ggtcctcctg gtccgcgtcc tccgccccca cgcgggcctc
     2821 tgcggtcaaa gccgccgctt ctgcgtggtc cgccatcgct ttcgcgtcga ggttctcgtg
     2881 gggctcctgc tggtgcggga gccggtgctg gtgctggtgt cataaatcta cgtaattagg
     2941 ataatttttt agtaatgctt tcaagaagca cacgtcatga gccgaggaat cggctactga
     3001 cgtacacttc ctggcgcaaa tgccggccac acccactgcg tccggcgata ccagtgcgag
     3061 actccggcga gtcgatcagt cggtgcagtg atgcgtgcga gcgacactcc gtgtatgtct
     3121 tcgggtagtg cgtacacgaa gtcgggtgcg tgcgtgaaga cggcaggata gtcagcagca
     3181 atgagtgcat ctacttctgc caggtcctca tcaacttttg cgggatcctg ttcaacgcgc
     3241 atagactcaa gaagtgtgtc aacggcgcgt gaagcgtaga gcgcaatatt aagtccggga
     3301 tcagtgcgct gcgaagagtc ccagaaggca aacaggtcgc ggtcacgtcc cactaccatg
     3361 ccaaataaca atgcgtcgta tgcacgcgga cgtatcaccg atgctgccaa gtcgcttggg
     3421 tcataatact cgatagctac cggaacaccg agtttctccc aatcgctccg tatggcttcg
     3481 gcgatagctt taagctcggg aacgttagag gtcttaagag tgacggtgag ctcaagatcc
     3541 tcatgcttcc atcgcttcgt ctcctcgtta tattcccatc cgttcttctc gagtatcgct
     3601 gacgccgccc caatggagtc ctcggatact tcaggagccg caatcgtgac gccgcttccc
     3661 ggaggtactg gtccccagac cggggtgccg tacccgcc
//
LOCUS       DAORIN010000064         3633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_5736_length_3633_cov_6.395472, whole genome shotgun sequence.
ACCESSION   DAORIN010000064 DAORIN010000000
VERSION     DAORIN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5736_length_3633_cov_6.395472"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..618
                     /locus_tag="PK109_02845"
     CDS             211..618
                     /locus_tag="PK109_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRH24500.1"
                     /translation="MKGYVTNIEQQALENDDFRRVLYTAKHCQLVVMSLPAGTDIGEE
                     VHDVDQFIRIEEGEGQSILDGVERDLTDGSAIVIPAGVRHNIKNTSGSDALKLYSLYA
                     PPHHKDGTIHPTKADAENDTEHFDGVTTDDLKS"
     gene            complement(620..1009)
                     /locus_tag="PK109_02850"
     CDS             complement(620..1009)
                     /locus_tag="PK109_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24501.1"
                     /translation="MKTFTRLLWLLPLLALIYMYGAQGVGVLTGSPEHYDILAALGWS
                     RDLTMALVWVSVVVDLGMMALLMFFPSATVFLIAGLWTWVPRIITLVAPGPENEFFES
                     LAVAVLALLSYLAYTRGHYLPFRKLLK"
     gene            complement(1041..1502)
                     /locus_tag="PK109_02855"
     CDS             complement(1041..1502)
                     /locus_tag="PK109_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRH24502.1"
                     /translation="MTNDPVAIIKNDHKTVEGLFKEYEGLGDGAHVTKRKVVDQIIEA
                     LTIHADMEETICYPRFLKAFNKNKEDDKMVEEAYVEHAGAKNLLANLRTLQPEEAEFD
                     ASVKVLMEQIRHHVKEEESELLPTVKKEVPEEELAAMGDKMLAFKEARSAA"
     gene            1609..2028
                     /locus_tag="PK109_02860"
     CDS             1609..2028
                     /locus_tag="PK109_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRH24503.1"
                     /translation="MNESIIVEALIQAPLEKVWHDYTDPRAIMAWNAASDDWHTTKVE
                     NDVRTGGAFMSRMEAKDGSVGFDFTGTYDEVIPQEKIAYTMTDGRNAVITFAPVDGAV
                     KVVVTFDPETENPHDMQRAGWQAILDSFKKYCEEHAA"
     gene            2034..2507
                     /gene="bcp"
                     /locus_tag="PK109_02865"
     CDS             2034..2507
                     /gene="bcp"
                     /locus_tag="PK109_02865"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRH24504.1"
                     /translation="MALPAVGKKAPTFSLPDQDGKVRTLADYAGKWVLLYFYPKDDTP
                     GCTIEACTIRDQFQDFKKIKAVVLGVSTDSIASHKKFVDAYELPFTLLADVDKKLVNK
                     YGVWGEKNMMGRTYMGTKRTSFLISPEGKIAKVYENVKPPKHAAEVLSDLRAIAV"
     gene            2504..3049
                     /locus_tag="PK109_02870"
     CDS             2504..3049
                     /locus_tag="PK109_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRH24505.1"
                     /translation="MKEFVVGLDMNVAHVLVGMRSEAATNFFLFITQLGNASFILIFS
                     LAVAGYFWWRTHSYFLPFLVSIIGSSLSVTIIKEVIDRARPGTDIAFYAEHLFSFPSG
                     HAASAVAAFGFVAYALMRMSQGSVTKKLVLGTATILILLIAFSRLYLGVHYLSDVIAG
                     ILVGGFWVVVGALLHHYVRRI"
     gene            3046..>3633
                     /locus_tag="PK109_02875"
     CDS             3046..>3633
                     /locus_tag="PK109_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRH24506.1"
                     /translation="MSTDAHHYDVIVIGGGASGLMAAGTAAAAGKRVLLFEKNAKLGE
                     KLRITGGKRCNITNAEDDLKVLLGKYGDAEQFLYSAFNEFGVKDTFTFFESKKLPLVV
                     EANKRAFPKSQSAADVVKTMVDYINKGKVTVKLKTPVRQILTEEGRIKAIVASGGEYT
                     AESYIFATGGVSHPETGSTGDGFGWLKALGHTVSDP"
BASE COUNT          933 a          914 c          956 g          830 t
ORIGIN      
        1 atttatgatt tctataaatg tacttggcgg tggcggcacg gactgtgtag atgaggcagt
       61 gtcagcgacc agaggttccc cctcactgcc gcccgaaaag ggaaaggata gggcggttcc
      121 cataagtatg ccgagggcga ccaatgtgaa ggcaaagggg acctttttca tacgctagaa
      181 aagtgtagca ccgaggcata tactatctat atgaaaggat acgtcaccaa catagaacag
      241 caggctcttg aaaacgatga ttttcgccgc gtcctctata ccgctaagca ctgtcagctg
      301 gtcgttatgt cccttcccgc aggaacggac atcggtgaag aggtgcatga cgtcgatcag
      361 tttattcgca tagaagaagg agaaggccag agcatactcg atggcgtgga gcgcgatctt
      421 accgatggct cagccatagt tattcctgca ggcgtgcgcc acaacatcaa gaacacgtca
      481 ggaagcgacg cactcaagct ctactcgctc tatgcgccgc cgcaccacaa ggacggcacc
      541 atacatccga cgaaagcgga tgcggagaac gacaccgagc acttcgacgg cgtcaccacc
      601 gacgacctta agagctaatt cacttgagga gcttccggaa gggaaggtaa tgcccgcggg
      661 tatacgcgag gtaggagagg agtgcgagta cggcaactgc taacgattcg aagaattcat
      721 tctctggccc aggcgcgaca agcgtgatga tacgcggtac ccacgtccat agtcctgcaa
      781 taaggaagac agtggcagaa gggaagaaca tcagaagcgc catcatgccg agatcaacga
      841 cgacactcac ccacacgagc gccatagtga ggtcacgaga ccatccaagc gctgccagaa
      901 tgtcatagtg ctcgggagaa cccgtgagta cgcctactcc ctgagcgccg tacatatata
      961 tgagggcaag taaggggagg agccaaagaa ggcgtgtgaa agttttcatc gaaggatttt
     1021 accacctccg taagagctct ttatgcagca ctgcgtgctt ctttgaacgc cagcatctta
     1081 tctcccatcg ccgcaagctc ttcttccggc acttctttct taacggtagg gaggagttcc
     1141 gattcttcct cttttacgtg atgacgaatc tgttccatga gcaccttcac gcttgcgtcg
     1201 aactctgcct cctccggttg cagggtacgc aggttagcaa gtagattctt agcccccgca
     1261 tgctccacat acgcttcctc caccatctta tcgtcctcct tattcttatt gaaggcctta
     1321 agaaagcgcg ggtagcagat tgtctcttcc atgtctgcgt ggatagtgag tgcttcgata
     1381 atctgatcaa cgactttgcg tttggtcaca tgcgcaccgt cgccaagacc ttcgtactct
     1441 ttgaagagac cctcaaccgt cttatggtca ttcttaatta ttgcgacagg gtcgttagtc
     1501 atgaggacag ggtattagtg cgataaagct gcaccatacg tgagttctca tgaatgcagg
     1561 gtgaatggaa gagtcggcaa acgcttacgg aattgctaca cttcctgcat gaacgaatcc
     1621 ataatcgtag aagcgctcat acaagcgccc cttgaaaagg tctggcacga ctatacggac
     1681 ccgagagcca tcatggcgtg gaatgccgcc tcagacgact ggcacaccac aaaggtagag
     1741 aatgatgtgc gtaccggcgg cgctttcatg agtcgtatgg aagcaaaaga tggaagtgtg
     1801 ggatttgatt ttactggcac gtatgacgaa gtcatcccgc aggagaagat tgcgtacacc
     1861 atgaccgacg gaagaaacgc agtcatcact ttcgcgcccg tagacggtgc ggtaaaggtc
     1921 gtcgtcactt ttgacccaga aacggagaac ccgcatgaca tgcagcgtgc gggctggcag
     1981 gcaattctcg acagctttaa gaagtactgc gaagagcacg cagcataata ggtatggcac
     2041 tacccgctgt tgggaagaaa gctcctacat tctccttgcc tgatcaagac ggaaaggtac
     2101 gcacccttgc cgactatgcc ggcaagtggg tgctcctcta tttctatcca aaagacgata
     2161 cgccaggatg caccatcgag gcgtgcacca ttcgtgatca gttccaggac ttcaagaaaa
     2221 ttaaagcggt agtgctaggc gtctctactg actccattgc aagccataag aaattcgtag
     2281 atgcgtatga gctcccattc acgcttcttg cagacgttga taaaaagctc gtaaacaagt
     2341 atggggtgtg gggcgaaaag aacatgatgg gaagaacata catgggcacc aagcgcacct
     2401 cattcctcat atctcccgaa ggaaagattg cgaaggtata tgagaacgta aagcctccga
     2461 aacatgcggc agaagtgctg agtgatttac gtgcgattgc agtatgaaag aattcgtcgt
     2521 cggtcttgat atgaatgttg ctcacgttct cgtgggtatg cgaagcgaag cagctaccaa
     2581 tttcttcctc ttcattactc agctagggaa cgcatctttc atacttatct tttcactcgc
     2641 agtggcaggc tatttctggt ggcgcaccca ctcgtacttt ctcccgttct tggtctccat
     2701 tattggcagt tcgctttcag taacgattat taaggaggtt attgatcgtg cacgtccggg
     2761 gactgatatc gcattctacg cagaacatct cttctcattc ccgagcggac acgcggcaag
     2821 cgccgtcgct gcgtttggct ttgtcgcata cgcactcatg cgtatgagtc agggaagcgt
     2881 cacaaaaaag cttgttctag gaactgcgac catcctcatc cttctcatcg cctttagtcg
     2941 cctgtatctt ggcgtccact atctctccga tgtgattgcc gggatactcg tgggcgggtt
     3001 ctgggtagta gtcggggcgc tcctccatca ctacgttcgg cgcatatgag tactgacgca
     3061 caccactacg atgtcatcgt cattggcggc ggggcgtcag ggcttatggc ggcaggcacc
     3121 gcagcggcag caggtaagcg ggtgctgttg tttgaaaaga acgccaagct tggtgagaaa
     3181 cttcgcatta cgggcggtaa gcgctgcaat ataacgaatg ccgaagacga tctcaaagta
     3241 ttacttggaa agtatggtga cgcagaacaa tttctgtact ccgccttcaa cgagtttggt
     3301 gtgaaagata ccttcacttt cttcgaatca aagaaattac cactcgtcgt tgaagcgaat
     3361 aagcgagcgt ttccaaaaag ccaaagtgcc gcagacgtag tgaagactat ggtggactac
     3421 atcaataaag gaaaggtgac ggtaaagctt aagactccgg tacgtcagat cctcaccgaa
     3481 gaaggacgca tcaaagccat cgtcgcaagt ggtggtgagt acaccgctga aagctatatc
     3541 ttcgcgacgg gtggtgtctc acatccggag acggggtcta ctggggacgg gttcggatgg
     3601 ctcaaagcac tcggacacac ggtgagcgac cct
//
LOCUS       DAORIN010000065         3611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_5812_length_3611_cov_5.584927, whole genome shotgun sequence.
ACCESSION   DAORIN010000065 DAORIN010000000
VERSION     DAORIN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_3611_cov_5.584927"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK109_02880"
     CDS             <1..448
                     /locus_tag="PK109_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24507.1"
                     /translation="GKGKNTGTPFSGVMKAPRDTVPFESITKALGKDWRTTPVSCPQP
                     GGYGGAMGPTQFIPSTWMLFDARLKAALGVPATNPWNAEHAIMATGLYLQDVGGTGGT
                     YTAEHNAACRYYSGGACSGGAISAYGDSVVAKANAYQRDIDFLKDN"
     gene            519..>3611
                     /locus_tag="PK109_02885"
     CDS             519..>3611
                     /locus_tag="PK109_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24508.1"
                     /translation="MNHLSRLFVLALFLLTPFVSSAQETARPYIPRVLIHDVSVEKAT
                     YAQGETVNGSFVITNADTASAGGIVYVVRLVGGYDDTSIWSSEYVTQRSEVFALGPNE
                     EVTRTFSIAVPQMLAGEDFGVEVQAELASGFPLGWDAARIAINGTATYLTVDDAYVLI
                     GNRDFHTEEGPTLHEGQTGSLLAKVKNATAEAITLHPVITFVSMEGVTNEVQQDALTI
                     EAGKDATVSVPLAATTPGVYTATLRLEDAGGSVRAPLLNYRFIVAGATARIQTIATGD
                     TVWGSGDTVDVVVSISGTPPDINPNGAPREQSDTTAELRLTLKNEWGIAVAESSTEVD
                     IATSKSPIIPMTLSTGASALLARAELVKDGKTISLLETPLSAGYKEAQAKVVTLLVLL
                     VIIILAVIAAFVFRKRDAMNAFVFGRASISACVVGFLAALFALLLWLGVLSVPAAHAV
                     GSGNCFDPENPNSEYFCDGGDPGGGDPGPYVPPTYWYASYVRDGGIWSEFVRNNLPIW
                     DRTGSQYRVRNYVYTPPQALTETSYNVGLGYNASLPINIGSAFKTTWDYTRQQGRLTD
                     PVSGNQYVDLVSDASIYSLPATTYFVTPGGWTSAGSSDTATPFIYLNALRLPITPGGS
                     FYIDGTVSYYECTNMLVGFKLEGTFQGVARQQTIGASGTHGHTISSASKRFTLGPFTA
                     PSTPGTYRLTLNSGWTFGHTPLGNTDIGHIDIDVGGPVATLSPSPSSIVTGSSSTLTA
                     SCQNSTIASIDNGVGTVNATTDTRSVAPVANTTYTLTCEDAQGRTAQATAAVTVFDTP
                     LMATCTAAPSSADSGQTVNFSSSVSGGTGNYSYSWRESGNVFGTNSSASKSFSSNGTK
                     TITLTVTDLGPAPTSSFSASPTSVVSGNSSTLSWTSSNTSSCTGTGFSTGSATSGSVV
                     VSPNTSTTYSLACTGAGGSVNKSATVTVTAPPPAPTSSFAASPTSITSGGSSTLSWSS
                     TNATSCTGTGFSTGGATSGAATVSPTTTSSYSLSCSGAGGTVNKSATITVTAGGGGGG
                     G"
BASE COUNT          824 a          965 c          958 g          864 t
ORIGIN      
        1 tggtaaaggg aagaacaccg gtacgccatt ctctggcgtc atgaaggcac cgcgcgacac
       61 cgtaccgttt gaatctatta ctaaagcact tggcaaagac tggcgcacaa ctccagtttc
      121 gtgtccgcag ccgggagggt acggaggcgc tatgggtcct acccagttca ttccttcaac
      181 ctggatgctt ttcgacgcac ggcttaaggc agcgcttgga gttcctgcta ctaatccatg
      241 gaatgcagag catgccatca tggcaaccgg tctctacctt caggatgtag gaggtaccgg
      301 aggaacctat acagctgagc acaatgctgc ttgccgttac tactccggcg gtgcctgctc
      361 gggtggagca atttcagcct atggtgattc agtggtggct aaggcaaatg cgtatcagcg
      421 ggatattgat ttcttgaagg ataattaagc cacctatcac tttcttctat ccacagtagg
      481 aatgattttt tgatgagtat ctccgctaca gtagtagtat gaaccatctt tctcgcctgt
      541 tcgttcttgc actcttctta ttaactccat tcgtttcgag cgctcaagag acggcacgtc
      601 cctacatacc acgtgtcttg atccatgatg tatcggtaga gaaggcaact tacgcgcaag
      661 gagagacggt gaatggttct ttcgtcatca cgaatgcgga tactgcatca gcgggcggta
      721 ttgtctatgt cgtacgactt gtcggaggat atgacgatac gtctatttgg tcatcggaat
      781 acgttaccca acggtcagag gtatttgctc tcggtccaaa cgaagaggta acccgtacct
      841 tttctatagc tgttcctcag atgcttgctg gagaagactt cggagtggaa gttcaggcag
      901 agcttgcgtc tgggtttccg ctcgggtggg acgcagcacg catcgccatt aatggtactg
      961 cgacgtatct caccgtagac gacgcgtacg tgcttatcgg caatcgagat tttcacactg
     1021 aagaaggtcc gaccttgcat gaaggtcaga caggatcgct actggcgaaa gtgaagaacg
     1081 caacggctga ggcgattaca ctccacccgg tcatcacttt cgtatccatg gaaggggtga
     1141 cgaacgaagt ccagcaagat gcactcacga tcgaagcagg caaggacgcg accgtttctg
     1201 ttcctcttgc ggcgaccacg cctggcgtct acacggcaac acttcgtctc gaagatgctg
     1261 gaggaagcgt acgtgcgccg cttttgaact accgctttat cgttgctgga gcgaccgcgc
     1321 gtatacaaac catcgccacg ggggatacgg tgtgggggag cggcgacacg gttgacgtgg
     1381 tggtatctat ttccggaacg ccaccggaca taaatccaaa cggggcacca cgtgagcagt
     1441 ccgacacgac tgccgagctc cggctcacgt taaagaatga gtggggtata gccgtcgctg
     1501 aatcttcgac tgaagtcgat atagcgacga gcaagagtcc gatcattcct atgacattgt
     1561 ccacaggagc aagtgcgctc ctcgcccgag cggaactcgt gaaggacggt aagacgattt
     1621 cccttcttga gaccccactt tctgccggct ataaagaggc gcaggcgaaa gtagttacgc
     1681 tcctcgtgct tcttgtaata attattcttg cagttatcgc agcgttcgta ttcagaaagc
     1741 gcgatgcgat gaatgcgttc gtgttcggcc gggcaagtat cagtgcatgt gtggtcggct
     1801 tcctagcggc actttttgca ctgctgcttt ggctcggtgt cctgtccgtt ccagcagctc
     1861 atgcagttgg ttctggtaat tgctttgatc ccgagaatcc aaatagtgag tatttctgcg
     1921 acgggggaga ccctggcggt ggagatcccg gaccgtacgt gcctcctacg tattggtacg
     1981 ccagttatgt acgcgacggc ggtatatgga gtgagtttgt gcgtaacaac ctcccgatat
     2041 gggacaggac gggcagccag tacagggtac gcaattacgt gtacacgcct ccacaggcac
     2101 tcaccgaaac ttcgtacaat gtcggactcg gctacaatgc ctcacttcct ataaacatcg
     2161 gaagtgcatt caagaccaca tgggactata cgcgtcagca aggacgtctt accgatccgg
     2221 taagtggtaa ccagtacgta gacttagtct cggacgcatc catttatagt ctccctgcta
     2281 ctacctattt cgtgactcca ggagggtgga cgtcagctgg gtcatcggat actgcgacac
     2341 catttatcta cctcaatgcg ctccggctcc cgattactcc cggcgggtcg ttctacatcg
     2401 atggaaccgt atcgtattac gaatgcacta acatgctggt cggatttaaa ttggaaggca
     2461 cattccaagg cgtagcacgg cagcagacta ttggtgcttc gggaactcac ggtcacacca
     2521 tatcaagtgc gtccaaacgc tttacgcttg gccccttcac agcgccttca actcccggca
     2581 cctatcgact cactctcaat agcggatgga ccttcggtca tacaccactt ggaaacacgg
     2641 atataggcca tatcgacata gacgtgggag gtcctgtagc aaccctttcg ccgtccccct
     2701 cgagtattgt cactggttct tcttcgaccc ttaccgcttc gtgtcagaac tcaacaattg
     2761 cgtctattga taatggggtg gggacggtaa atgcaacgac tgacacaaga agtgtggctc
     2821 cggttgcgaa caccacctat acccttactt gtgaggacgc acagggaaga acagcgcagg
     2881 caactgctgc cgtgaccgtg tttgatactc cgttaatggc aacctgcact gcggcgccgt
     2941 cttcggcgga ttcgggacag accgtgaact tctcatcttc agtatcaggc ggcacaggta
     3001 actacagcta ttcgtggagg gaatcaggaa acgtcttcgg caccaattcg agtgccagta
     3061 agtcgttctc ttcgaatggt acgaaaacca ttacacttac cgtgaccgat cttgggcctg
     3121 cgccgacctc aagtttctct gcgagtccaa caagtgttgt ttcaggtaat tcatccacgc
     3181 tgagctggac gtcctcaaac accagctcct gtacgggtac cgggttctca acgggtagcg
     3241 caacgtccgg atctgttgtc gtttctccga acacctcaac cacgtactcg cttgcgtgta
     3301 ctggtgcagg aggttcagtg aacaagagtg cgacggtcac tgtgacggca ccacctccag
     3361 caccgacttc ttcatttgct gcaagtccaa ccagcatcac ctcaggaggt tcctcaacgc
     3421 tctcctggtc ttcaaccaat gcaacgtcct gcaccggcac tgggttctct actgggggtg
     3481 caacttcggg agcggccaca gtttcgccga cgactaccag ctcctattcg ctttcgtgta
     3541 gcggtgcagg aggtacggtg aataagagcg caacaatcac cgtgactgct ggaggaggcg
     3601 gcggaggagg a
//
LOCUS       DAORIN010000066         3597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_5866_length_3597_cov_5.166290, whole genome shotgun sequence.
ACCESSION   DAORIN010000066 DAORIN010000000
VERSION     DAORIN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5866_length_3597_cov_5.166290"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK109_02890"
     CDS             complement(<1..594)
                     /locus_tag="PK109_02890"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRH24509.1"
                     /translation="MTILTRAVACFSLIIFLSAGLPIEAFAAGSGAIDASTLTTKDTT
                     PALTGTASGVSKVRILVENGTGKDMYKSNALKVKDGKWRATVSKKLPVGNYTVTLYGP
                     KGVSKNTLAKKTLSILPANGATLAGGTGGSLSVSPILLLTGGNVAHGGSVPVAYVKVS
                     NTGSASTTINGFTLTENGSAPDDVVIGFTTNDDKGGSR"
     gene            complement(719..952)
                     /locus_tag="PK109_02895"
     CDS             complement(719..952)
                     /locus_tag="PK109_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24510.1"
                     /translation="MKNLFLGALALAALVAAPAATFAATYAYVNTAGEVMTMEASSAE
                     QALSTAPNLHVHSGVMTVGTDDANVVGDTVPGT"
     gene            complement(1097..2563)
                     /locus_tag="PK109_02900"
     CDS             complement(1097..2563)
                     /locus_tag="PK109_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24511.1"
                     /translation="MRTLFSVVLGLLLVGTTPLSALAASLNVSPGQASVRAGDIVTVR
                     LVANTQGVAINQGEGTLRFPTDMLDVVGTSKSGSIFTLWVEEPGYSNTSGTVHFDGGV
                     PTPGYTGSAGTVLSITFHAKKAGTATLTLTDTALRANDGMGTDVLSASNGGTITIAET
                     AVTPAPTPTPSTPATPTPSTGSTGTGAISNLSSPTHSDPNAWYQNQNPLITWELPAGA
                     TAVQTIVDADENATPSVDYKPAIVEKRISNLDDGVRYFNVRARTASGWGPVAHFRLQI
                     DSVAPDLASDILLDYDAARQELLISGIHAEDTTSGISHYLLAVDGSEPVRIEAEEVVN
                     GSYHFRYSKSGVHSIVLSAVDKAGNHTEAAGSFNIPTALANQTLFSIGSLNITLAWFI
                     FLILLISLLSLAAAAAAWYKLFIVRTGAKSRIEKRDKLLHRSLRIYKEELERHLRTLE
                     RAGSRRELTDEEAELNEDLKKNVDDLERYLLKEFKKYD"
     gene            complement(2567..3400)
                     /locus_tag="PK109_02905"
     CDS             complement(2567..3400)
                     /locus_tag="PK109_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24512.1"
                     /translation="MKKLSFVTLLLALVWLPAYAAQLSIETPLAGAGDPFSLPVVLTP
                     EGVTLNVVEGVIQVPDGVVVDRINTAGSAFSLFMTGPSYVPASHAIEFTLGAPGGVST
                     NEPMLLFVIEGHAQVPGSYPFKGTVTGYENDGVGTALAIAVAPATLSVGPEGSVHVDP
                     LPESAPTSLIAEVGRDASLFEGKWFATFFGGMSGSSVDHYAVREGWWRAEVPADRYYV
                     LQDQSRSSTLWVTAVGENGKRVTVAVAPENPWMERLLAAAIILTLGIIASLVWRRMKR
                     S"
     gene            complement(3402..>3597)
                     /locus_tag="PK109_02910"
     CDS             complement(3402..>3597)
                     /locus_tag="PK109_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRH24513.1"
                     /translation="GDSCNSIIGDINCDGRVSAVDFSIMLAFWKTQAPFRNPRVDLNT
                     DARVDAVDFSILLYHWGRTR"
BASE COUNT          864 a          908 c         1009 g          816 t
ORIGIN      
        1 acgagatccg cctttatcgt cgttcgtggt aaatccgatg accacatcgt cgggagcgga
       61 tccgttctcg gtgagcgtaa agccgttaat cgtggttgag gcagaaccgg tgttcgagac
      121 cttcacatac gcgacaggga ctgaaccacc atgagcgacg ttgccgccgg tgagaagaag
      181 aataggagac acagaaagag atccgccggt tcctcctgct aaagtcgctc catttgccgg
      241 aagaatactg agggtcttct tcgcgagggt gttcttgctg actcctttag ggccatacaa
      301 cgtgacggtg taattcccca ctggcagttt tttacttacc gtcgcgcgcc acttcccatc
      361 ttttactttg agtgcattgc tcttatacat gtctttcccc gtaccatttt ctacgagtat
      421 ccgtactttg gagacaccgg aggcagtgcc cgtaagagcg ggcgtcgtat ctttcgtcgt
      481 aagcgtagag gcatcgatag ctcctgagcc tgcagcaaat gcctcaatgg gaagacctgc
      541 ggataagaag atgatgagag agaaacaagc gacagcgcgg gtaaggatgg tcatagaaaa
      601 agtatatgag gagatgtgat tatgagcgag tttatgaagt acttcatgaa agaaacgacg
      661 aactctcgga gcgtgggctt cgagagttca gacgattcaa gcagagcttg atgacggatt
      721 aggtaccggg gacggtgtcg ccgacgacat ttgcgtcatc ggtgccaacg gtcataacgc
      781 cgctgtggac gtggaggttt ggtgcggtgg agagggcctg ctcagcagag cttgcttcca
      841 tcgtcatcac ctcacctgcc gtgttcacat aggcataggt tgctgcgaac gtcgctgccg
      901 gagcggcgac gagcgctgca agggcgagcg cgcccaagaa tagattcttc atggaattca
      961 aaagttaatt atgttagtgc ctgagctcct aactcaggga gcatatacct atatgcatga
     1021 gggaattatg aagaaattca tgaagtatga ccatagggga tacatgtcag ggaagtgact
     1081 tccctgtcat atggtcctag tcgtacttct taaactcctt caacagatac cgttcaagat
     1141 catccacatt cttcttgaga tcctcgttga gttccgcttc ttcgtcggtg agctcacgtc
     1201 gggagccagc gcgctcaagg gttcggagat ggcgttctag ctcctcctta tagatgcgaa
     1261 gcgagcgatg gaggagctta tcgcgcttct ctatgcgtga cttagcaccc gtccggacga
     1321 tgaatagctt gtaccaagcg gcagcggcgg cagcgagcga gagaagtgaa atgaggagga
     1381 tgaggaagat aaaccatgcg agcgtaatgt tcagactacc gatgctaaac agggtctggt
     1441 ttgcgagagc cgtcgggata ttgaaggaac cggcagcttc tgtgtggttc cctgctttat
     1501 ccactgccga aagcacgatt gaatgcactc ctgacttgct gtagcggaag tgatacgaac
     1561 catttaccac ttcttctgcc tctatacgaa ccggttcgct cccatccact gcaagaaggt
     1621 agtggcttat accagacgtc gtgtcttcag cgtgtatgcc agagatgagt agctcctgtc
     1681 gcgccgcgtc atagtcgaga agaatgtcag aagcgagatc gggggctact gaatcgatct
     1741 ggagacggaa atgcgcaaca ggaccccacc cgcttgccgt acgagcacgc acattgaagt
     1801 accgcacgcc gtcatcaagg tttgaaatac gcttctctac aatagccggc ttgtaatcaa
     1861 cggacggagt ggcgttttcg tcagcgtcaa caatggtctg tactgctgta gcgcctgcag
     1921 gaagctccca ggtaataagc gggttctgat tctgatacca cgcatttgga tcgctatgcg
     1981 ttggtgaaga gagatttgag atagctcccg taccagtgct gccggtagat ggagtaggtg
     2041 ttgccggagt ggatggcgtt ggtgtaggcg ccggcgtgac cgcagtctct gcaatagtga
     2101 tagtgccgcc attagatgcg gacaacacgt ccgttcccat accatcgttt gcacgaagtg
     2161 ctgtgtccgt aagggtgagc gttgccgtac ccgctttctt tgcatgaaaa gtaatagaaa
     2221 gtacggtgcc tgcagatccg gtatatccgg gtgttggaac gccgccatca aaatgcacgg
     2281 tgccggacgt gttcgagtat cccggctcct ccacccagag ggtaaatatg gaacctgatt
     2341 tactggtgcc gacgacatcc aacatatcgg ttgggaatcg gagcgttccc tctccttggt
     2401 tgatggcgac tccttgagtg tttgcaacta agcgtaccgt gacaatatct ccggcgcgca
     2461 ccgatgcctg accgggagat acgttaagag atgcagcaag tgcagacaat ggcgtggtcc
     2521 ccacaaggag tagtcccagc actacgctaa agagtgttcg catagcttag gagcgcttca
     2581 tccggcgcca cacgagtgag gcaatgatgc caagcgtgag aatgatagct gcggcaagga
     2641 gacgttccat ccatgggttc tctggtgcta ccgctaccgt cacgcgcttc ccgttttctc
     2701 ccactgccgt cacccaaagc gtactgctgc gtgactgatc ctggaggaca tagtaccggt
     2761 ctgcaggtac ctctgcgcgc caccatcctt cgcgcacggc gtaatggtca accgagcttc
     2821 ctgacatgcc gccaaagaag gtggcgaacc atttcccttc gaaaagtgag gcatcacggc
     2881 cgacttctgc gataagtgag gtcggagcgc tttctggaag cgggtccaca tgaacgctac
     2941 cttcaggtcc aactgaaagc gttgctggag caaccgcaat tgcgagcgca gtccccactc
     3001 catcattctc gtatccggta acggttcctt tgaagggata ggaaccagga acttgtgcat
     3061 gaccctcgat aacaaacagg agcatcggct cgttcgtcga aacgccaccc ggagccccga
     3121 gcgtaaattc aatagcatga gaagcgggca cgtagcttgg accggtcatg aataaactaa
     3181 atgctgaacc tgccgtattg atgcgatcaa ccacgacgcc atctggcacc tgtataacgc
     3241 cctcaacaac attcagagtt actccttctg gagtaaggac gaccggaagc gagaaggggt
     3301 cgccggctcc tgcaagcggt gtctcaatag aaagttgggc agcgtaggcg ggaagccaaa
     3361 cgagcgcgag gaggagtgtg acgaaagaaa gctttttcat attatcgagt tcttccccaa
     3421 tgatatagga gtattgagaa gtcgacagcg tcgacccgcg catctgtgtt taagtcaacg
     3481 cgcggattgc ggaatggtgc ctgcgtcttc cagaacgcga gcatgatgga gaagtccacg
     3541 gcactcacgc gtccatcgca gttaatgtca ccgattatgg aattacacga atccccg
//
LOCUS       DAORIN010000067         3483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_6216_length_3483_cov_5.591015, whole genome shotgun sequence.
ACCESSION   DAORIN010000067 DAORIN010000000
VERSION     DAORIN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6216_length_3483_cov_5.591015"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PK109_02915"
     CDS             complement(<1..454)
                     /locus_tag="PK109_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRH24514.1"
                     /translation="MTTTQESPFVEKYLTPIAVLLGAIIIAVAFAFGTGGTRPTTEGT
                     QGAAVDIKNLSIGSSPVLGSATAPVTMAVWFDYQCPFCKKYDMETVAQVYANYVESGK
                     VKIVLKDFQFLGSDSDAAALFARAVWDAYPDKFYPWFQAMMEAQDDEGD"
     gene            complement(568..756)
                     /locus_tag="PK109_02920"
     CDS             complement(568..756)
                     /locus_tag="PK109_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24515.1"
                     /translation="MNNNNFGNTLLVIILVLIVGFGVWYFTSRTGAPAEPQGPGIQVD
                     ITGPSGDDGTPDQGPGDN"
     gene            complement(767..1195)
                     /locus_tag="PK109_02925"
     CDS             complement(767..1195)
                     /locus_tag="PK109_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24516.1"
                     /translation="MATKKSTTKKAGTKKKGIPAAGIAAGVVAAGAAAGAAYYFYGAK
                     GAKQHRAKARKWAGAMKREVIKEAKMIQKLDDKVIHGVIDRVANTYRAAKSVNPEEIR
                     EAAMELKNNWKKIQAEMKGARSTVKRTAKKAVKKSAKRRA"
     gene            complement(1242..1544)
                     /locus_tag="PK109_02930"
     CDS             complement(1242..1544)
                     /locus_tag="PK109_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24517.1"
                     /translation="MSDFLKMDIFFFVATIGTIVTSILLATILFYGIRVLRGIERILG
                     EVEEEAKALRADIQEVRAVARKEGSKLAGLLESFERMLRGVFGIKKRRSPKKKTDS"
     gene            1597..1932
                     /locus_tag="PK109_02935"
     CDS             1597..1932
                     /locus_tag="PK109_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HRH24518.1"
                     /translation="MIGFDHHLHLRKRVYKKLEQYPNPDTLKRFLDKAMVFVGIAGPL
                     AMLPQVYQVFATQDAKGLSIITWFIWTLTSALWLTYGVIHKETPIVVSNSIYIALHVT
                     IVVAILVFG"
     gene            complement(1981..3303)
                     /locus_tag="PK109_02940"
     CDS             complement(1981..3303)
                     /locus_tag="PK109_02940"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRH24519.1"
                     /translation="MTTTYIVRKNSRNDLRDRPGYERFAQKRSGGGAGGPRRNSGGPS
                     RGGPRSGGNSRGGGGSRGRGGRQQEMAVDLSKLINKAKPLADEAPFVPKHSFADFPID
                     SMLKENILAKGYMNPTPIQDESIPHVLKGEDVVGLANTGTGKTAAFLIPLIDKVLKMR
                     ALGKEENILVMVPTRELAIQIETEFWGFAKRLSMNAVVCVGGAGIEPQIRVLRRRPAF
                     VIGTPGRLKDLMERRELDLSKFSTVVLDEADRMLDMGFIDDMRFILAKMRGDRHTLFF
                     SATMDKSIERLVNDFLKNPVRISVKTRDTAATIDQDVVRVPRGRKLETLIDLLKNPEF
                     DKALVFGRTKHGVERLARDLSRVGIKAESIHGDKTHGKRQKALGLFKQNHVNVLVATD
                     VAARGLDISGVSHVINFDLPATYEDYTHRIGRTGRAGKTGVALTFIEE"
BASE COUNT          820 a          941 c          863 g          859 t
ORIGIN      
        1 catcgccttc atcatcctgt gcttccatca ttgcctggaa ccaaggatag aacttatcag
       61 ggtacgcatc ccatacggca cgtgcaaaga gtgctgccgc atcagagtcg cttccgagga
      121 actggaagtc cttaagaaca atctttacct tgccgctctc gacatagttc gcgtatacct
      181 gcgcaacggt ttccatgtcg tacttcttgc agaacggaca ctggtagtcg aaccagaccg
      241 ccatagtgac tggagctgtt gcgcttccga gtaccggcga gctcccaata gaaagattct
      301 taatatcgac agcagcaccc tgcgtgcctt cagtagttgg acgcgtgccg ccggtgccaa
      361 aggcaaaagc gacagcgatg ataatagcgc cgagaagcac tgcaatagga gtcagatatt
      421 tctcgacgaa cgggctttct tgagtagtgg tcatgatgcg aaacacatat tattttgcaa
      481 tccctctagc atacctccgg gggccaactc gtccaatagc tagaaaacgc cccgaaatac
      541 ggggcgtttt cgcgagctaa ggacgaacta gttgtccccc ggaccttgat caggggtacc
      601 gtcgtccccg gaaggtcccg ttatatctac ctggataccg gggccctgag gctcagcagg
      661 cgctccggta cggctcgtga agtaccacac accaaagcct acgatgagca cgagaataat
      721 aacgagaagc gtgtttccga aattattgtt gttcatagag ggtgagttat gcgcgacgtt
      781 ttgcggactt cttcaccgct ttctttgccg tccgctttac ggtagagcgt gcgcctttca
      841 tctccgcctg aatcttcttc cagttattct ttagttccat cgccgcctcg cgaatttctt
      901 ctggattcac tgacttcgct gcgcgatacg tgtttgcaac acgatcaatg acgccatgga
      961 tgactttgtc atctaatttc tgaatcatct tcgcttcctt aataacttca cgcttcatag
     1021 cgcctgccca cttacgtgct tttgcacgat gctgctttgc ccccttcgct ccatagaaat
     1081 aataggcagc acctgctgcc gctcctgcag caacgacgcc tgcggcgatg cccgctgcag
     1141 gaatgccctt cttctttgta cctgctttct tggttgttga tttttttgtt gccatagaac
     1201 tgtagagtga gtgaaagcgg gttaagactt catgaagaac ttcatgagtc ggtcttcttt
     1261 tttggagagc gtcgcttttt gatgccgaac acgccacgca acatacgttc aaaggactca
     1321 aggagacctg cgagttttga tccttctttt cgcgctacag cgcgcacctc ctgaatatcg
     1381 gcccgaagag ctttggcttc ctcctccact tccccaagaa tgcgttcgat gccgcgcagg
     1441 acacgtatgc cataaaaaag aattgtggcg aggagtatgg aagtgacgat ggtaccgata
     1501 gtggcgacaa agaagaaaat atccatcttt aaaaaatcgc tcatgtaagg acagtgtagc
     1561 actagactag taatgagctg ttagaatgaa gcaagtatga ttggctttga tcaccacctc
     1621 cacctacgca aacgcgtata caaaaaactt gagcagtatc caaaccccga tacgttaaaa
     1681 cgatttcttg ataaggcgat ggtatttgtc ggcatcgcag gtccgctcgc catgctcccc
     1741 caggtgtatc aggtatttgc cacacaggat gcgaaaggac tgtccattat tacctggttc
     1801 atatggacgc tcacgagcgc cctctggctt acctatggcg ttattcataa agagacgcct
     1861 atcgttgtct ctaacagcat ctacatcgcc ctccatgtca ccatcgtggt cgctatcctg
     1921 gtgttcggat agataaagaa aaaccgcccg aatacttcgg gcggtttttc ttgggtgtaa
     1981 ttactcctcg atgaacgtaa gcgcgacgcc ggtcttccct gcgcggcccg tacggccaat
     2041 tcggtgggtg tagtcctcat aggtcgctgg caggtcaaag ttaatgacgt gcgagacgcc
     2101 cgagatatca agaccacgtg cggcaacatc ggtagcgacg agcacgttca catggttctg
     2161 cttgaagaga ccgagtgctt tctgacgctt accgtgcgtc ttatcaccat gaatgctttc
     2221 cgccttgatg ccaacgcggg aaagatcacg cgcaagacgt tcaacaccat gcttggtgcg
     2281 tccgaacacg agtgccttat caaactcagg attcttcaag aggtcgataa gggtctcaag
     2341 cttgcgtcca cgagggacac gaaccacatc ctgatcgatg gtcgctgccg tatcgcgcgt
     2401 cttcacggag atacgtaccg gattcttcaa gaagtcgttc accaagcgtt caatgctctt
     2461 atccatcgtt gcggagaaga agagcgtgtg acggtcaccg cgcatcttcg caaggataaa
     2521 gcgcatatca tcgatgaagc ccatgtcgag catgcggtcc gcttcgtcga gtacaaccgt
     2581 ggagaacttc gaaaggtcga gctcacggcg ttccataagg tccttcaagc gtcctggcgt
     2641 accgatgacg aatgctgggc gacggcgaag cacacgaatc tgtggctcaa tgcccgcgcc
     2701 tcccacacac acaacggcgt tcatagagag acgcttcgcg aagccccaga attcagtttc
     2761 aatctgaata gcgagttcac gggttggcac catgacgaga atgttctctt cctttccaag
     2821 agcgcgcatc ttcaatactt tgtcgatgag cggaatgagg aaggctgccg tctttccggt
     2881 tccggtgttc gcaagtccca cgacatcctc tcctttgagg acatgcggaa tcgactcgtc
     2941 ctgaatcggc gttgggttca tgtagccctt cgcaaggatg ttctccttaa gcatcgagtc
     3001 tatagggaaa tctgcgaacg agtgcttcgg cacgaatggt gcttcgtcag cgagcggctt
     3061 cgctttatta atgagttttg agaggtcgac agccatttcc tgctggcgtc caccgcgtcc
     3121 acgggaacca ccgccaccgc gggagttacc tccagaacga gggccaccac gagaaggtcc
     3181 gccgctattg cgacgagggc cacctgcgcc gcctccgctt cgcttctgag caaagcgttc
     3241 atatcccgga cgatcacgaa gatcattccg gctgttttta cgcacaatat acgtagtagt
     3301 catctaatta tgtaagtgaa tgctctgcgc taaagaggag aaaagctagc gcacgagctt
     3361 gatattcaca gtcgtcttta gaggttcacc cgaatctctc cttgctgctt tgcgggtggt
     3421 ctcgagtgaa tctcaagacg gaaaaccgat agaaaactgt tgtgccatta atatagcacc
     3481 tcc
//
LOCUS       DAORIN010000068         3466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_6272_length_3466_cov_5.090882, whole genome shotgun sequence.
ACCESSION   DAORIN010000068 DAORIN010000000
VERSION     DAORIN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6272_length_3466_cov_5.090882"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..875
                     /locus_tag="PK109_02945"
     CDS             51..875
                     /locus_tag="PK109_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24520.1"
                     /translation="MNMERPTNGSGESSEWVRAREKGLDMSQKARMERADALFPTVMY
                     TGTTSVFDEFSPEESGKGSGIAQVDGAVWATSDPEVASYFALFPLQDPYKYLIALVRK
                     ANLGEWYNSRATAEQLTIDNPKLKEAMGTFADTLAKHKISEKNWIGKLLSEKTLRTAV
                     LEDAERIADTFATITGTRVSITPVVMPLRINDADFIAKDFEGSSIEEITNLNDDYVRD
                     MRMEAERKESGGITLHNVADGIDKHSTIMAIKNYARIRSIHAAFDPDRKDEAGLMY"
     gene            complement(925..1212)
                     /locus_tag="PK109_02950"
     CDS             complement(925..1212)
                     /locus_tag="PK109_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24521.1"
                     /translation="MSDINLWIALPATYLLPFLYFRYRYNAHESVRIIGAIVLGVPWL
                     YALATFFYIGQSIEHFPENHNFWPTLGLVVLWGVLSFAGMVVGLNTDESPR"
     gene            complement(1358..1708)
                     /locus_tag="PK109_02955"
     CDS             complement(1358..1708)
                     /locus_tag="PK109_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24522.1"
                     /translation="MNHDESFKEKFIDPKTEESIFGELDPMEMGDENNETEEGEGITA
                     GTMQVVREKLKEYFVPEEYHTEVIDLIDEMMIGKHGKQLQEEAGFDDQLKMAISKVEE
                     RHGLSFDIEYDEED"
     gene            complement(1785..2633)
                     /gene="rplB"
                     /locus_tag="PK109_02960"
     CDS             complement(1785..2633)
                     /gene="rplB"
                     /locus_tag="PK109_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRH24523.1"
                     /translation="MKTYKPYTKSRRNMTTLPYRELLSGDRPTKALMKGKKQHAGRNS
                     FGRVTTRHQGGGHKKSYRDVDFSYNKKDVPAKVETIEYDPYRTGFIARVCYADGERRY
                     VLAPKELKVGDSFIVSETAPVKVGNRLPLGKLPTGTFVYNVELKVGAGARLARSAGNY
                     AEVVAHDAGFAQLKLPSTEIRKVSDNAWASIGAVGNENSRLVKFGKAGRSRWMGIRPT
                     VRGTAMNPVDHPHGGGEGRQGRGLRRAKSMWGKPTGKGQKTRTPKKYSNVFIVSRRKV
                     GKKNKG"
     gene            complement(2639..3013)
                     /gene="rplW"
                     /locus_tag="PK109_02965"
     CDS             complement(2639..3013)
                     /gene="rplW"
                     /locus_tag="PK109_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRH24524.1"
                     /translation="MALFEKKTKTTKKKSTKDVMVKAAGEKALANTKLENVIKAPWLS
                     EKALIGTEKGVYVFEVPRDTNKLQIKAAIERIYKVVPVKVNVVNLPRKAKSLRTKRGT
                     GFRAARHKAYVFLKKGETITFA"
     gene            complement(3017..>3466)
                     /gene="rplD"
                     /locus_tag="PK109_02970"
     CDS             complement(3017..>3466)
                     /gene="rplD"
                     /locus_tag="PK109_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRH24525.1"
                     /translation="RHGSTRSPIWRHGGITHGPRADRDYTVKINRKMRAAALVSLLSR
                     KYRDGEIILVDKLSFATPSTKAARAMMVAVAKGAGRDTFATKRANAAVIAFSKKDLVA
                     EKSLRNIGSLTLEEVRNLNPVDLVTKKYLVIENPAESFAILASRLTK"
BASE COUNT          823 a          902 c          884 g          857 t
ORIGIN      
        1 ctcataggac acagtatacg cgaaccaatt tcctacggta tgctatgccc atgaatatgg
       61 agcgaccaac gaatggaagt ggtgaaagct ctgagtgggt gagggctcga gagaagggac
      121 tcgatatgag ccagaaagcg cgcatggagc gtgcggatgc tctcttccct accgtcatgt
      181 ataccggaac cacttctgta tttgatgagt tctctcccga agaaagcgga aaagggtccg
      241 gaatagctca ggtagacggg gcggtctggg ctacctcaga cccagaagtc gctagctact
      301 ttgctctctt tccgttacaa gacccatata aatacctgat agcgctggta cgaaaagcta
      361 acctcggaga atggtacaac tcacgagcaa ctgctgaaca gttgacgata gataacccta
      421 aactaaaaga ggcgatgggc acttttgcgg atacgctcgc caaacataaa atctcagaga
      481 agaactggat tggtaagctg ctctctgaga aaacgctccg tactgcggtc cttgaagacg
      541 ctgaacgtat tgctgacacc ttcgctacta ttaccggtac ccgcgtttct ataacaccgg
      601 tagtcatgcc gcttcgaatt aatgatgctg actttattgc aaaagacttc gaaggctcct
      661 ctattgaaga gataacgaac cttaacgacg attatgtgcg agatatgcgc atggaggctg
      721 agagaaaaga aagtggcggc atcactcttc ataacgtagc cgacggaata gacaaacact
      781 ccactatcat ggctatcaag aattacgcgc gcatacgctc catacacgcg gcgtttgatc
      841 ctgatcgtaa agacgaggca gggttgatgt attaaaaaga acagccacgc ttgggtggcc
      901 gttcagtttc gtttcaagtt cgccctaccg tggagattcg tcagtgttta aaccgacgac
      961 cataccagca aagctcagca cgccccagag gacgacaagc ccgagcgtgg gccagaagtt
     1021 gtggttctcc ggaaaatgct cgatgctctg tccgatgtag aagaacgtcg cgagcgcata
     1081 gagccatgga acgccgagca caatagcacc gatgatacgc acgctttcat gggcgttgta
     1141 ccgatatcgg aaatagagaa acggtagaag gtacgtagcc gggagcgcaa tccagagatt
     1201 gatgtcgctc aagatctgag gaagatattt ctacattctc acttacctcg cgaatagttc
     1261 ccacccagcg cgaccatacc acaaaagctt cgaatcgtgc aaagaaacag ccccgcagat
     1321 gcgggactgt ttcttacttt tgctcctcac acacttttca atcttcttcg tcgtactcaa
     1381 tatcgaatga gagtccgtgt cgttcttcca ccttgctgat ggccatcttc aactgatcat
     1441 caaaccccgc ttcttcttga agttgttttc cgtgcttccc tatcatcatc tcatcgatga
     1501 gatcaataac ttccgtatga tattcctcag gcacaaaata ttccttcagc ttctcgcgca
     1561 ctacctgcat cgttcccgcc gtaataccct ctccttcctc cgtttcgtta ttttcatcac
     1621 ccatctccat cggatcaagc tcgccgaata tgctttcttc agtcttaggg tcaataaatt
     1681 tctctttaaa agattcatcg tggttcatac cgtaatgata cctcagttct cgactcccat
     1741 taaagaaaca gccccgcaga tgcggggctg tttctttttc tgaattatcc cttgttcttc
     1801 tttccaacct tgcggcgaga aacgatgaat acgttggagt acttcttcgg cgtgcgcgtc
     1861 ttctggccct tgccggtagg cttgccccac atgctcttgg cacgacggag accgcgaccc
     1921 tggcgtcctt caccgccgcc gtgtgggtga tccactgggt tcatagcagt accacgaacg
     1981 gttgggcgaa tacccatcca gcgagagcgt cctgccttac cgaacttcac gaggcgtgag
     2041 ttctcgttac cgacagcgcc gatagaagcc caggcattgt cagatacttt gcggatttca
     2101 gtagacggaa gcttcaactg tgcgaagcct gcgtcatgag cgacgacttc tgcgtagtta
     2161 ccagcagaac gtgcgaggcg cgcccctgct cctaccttga gttctacgtt atagacgaag
     2221 gtgccggtag gaagctttcc aagtggaagg cggttaccaa ccttaactgg ggcagtctca
     2281 gaaacgatga atgagtcccc taccttaagt tcctttggcg caaggacgta gcggcgctca
     2341 ccgtcagcgt agcaaacacg agcgatgaag ccggtgcggt atgggtcata ctcgatagtc
     2401 tctacctttg ctgggacatc cttcttgttg taggagaagt ccacatcgcg gtagctcttc
     2461 ttgtgaccac ctccctggtg acgagtggtg acgcgtccaa aggagttacg gcctgcatgc
     2521 tgcttcttac ccttcatgag ggccttcgta gggcggtcac cggagagaag ctcccgatac
     2581 gggagggtcg tcatgttgcg gcggctcttg gtgtacggtt tgtaggtttt catactagtt
     2641 atgcgaaggt gatggtctcg cccttcttaa ggaagacgta ggccttgtgg cgagcagcac
     2701 ggaagccggt accgcgcttg gtgcggagcg actttgcctt acgaggaagg ttcaccacgt
     2761 ttaccttcac tggtactacc ttgtagatgc gctcgatagc ggccttgatc tggagcttgt
     2821 tcgtgtcgcg aggtacttcg aatacataga cccctttttc agtaccaatg agtgccttct
     2881 cagaaagcca aggcgccttg atgacgttct caagcttggt gtttgcaagc gccttctcgc
     2941 ctgcagcctt taccatgacg tccttcgtgg acttcttctt ggtagtcttc gtcttttttt
     3001 cgaatagtgc cataaattat ttggtgaggc gtgacgcgag aatcgcgaag gactcagcag
     3061 ggttctcgat aacgagatac ttcttcgtaa cgagatccac tgggtttagg ttgcgcactt
     3121 cctcaagcgt aagggagccg atgttgcgaa gtgacttctc agcaacaaga tccttctttg
     3181 agaaggcgat gaccgcagcg ttagcgcgct tcgtagcaaa cgtgtcgcga ccagcaccct
     3241 tggcgactgc aaccatcatg gcgcgagcgg ccttagttga tggcgtagcg aatgagagct
     3301 tatcaacgag gatgatttct ccatcgcggt acttgcggga aaggagggag acgagcgctg
     3361 ctgcacgcat cttgcggtta atcttcaccg tgtagtcacg atcagcacgt ggaccatgcg
     3421 taataccacc gtggcgccaa attggggaac gggtagaacc atggcg
//
LOCUS       DAORIN010000069         3434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_6381_length_3434_cov_4.260728, whole genome shotgun sequence.
ACCESSION   DAORIN010000069 DAORIN010000000
VERSION     DAORIN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6381_length_3434_cov_4.260728"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK109_02975"
     CDS             complement(<1..405)
                     /locus_tag="PK109_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRH24526.1"
                     /translation="MKYFDAHCHIQFEAYDADRAEVVRAMQDAEVGGIIVGTDSTTSK
                     RAVQLADGKTMFASIGLHPNDKPQEGYDDAYYEELARDPKVVAIGECGLDYFRPENPE
                     AEKSRQKDVFARHIQLAIQTGKPLMIHARPSKG"
     gene            complement(466..657)
                     /locus_tag="PK109_02980"
     CDS             complement(466..657)
                     /locus_tag="PK109_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24527.1"
                     /translation="MATEKTVLERIAEEFVACKAVRKDAEQAIADSRQARRAPIELTG
                     LVHAGKFFAAVAVATAKPE"
     gene            complement(776..2212)
                     /locus_tag="PK109_02985"
     CDS             complement(776..2212)
                     /locus_tag="PK109_02985"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRH24528.1"
                     /translation="MRRYLTTTLPYVNADPHIGNALEFVQADALARAWRLQGDEVFFN
                     TGTDEHGQKILQAAEKAGKDVHDYVDHFSGEFKKLGSALDLSYDAFIRTTDPAHYAAA
                     QEIWRRCDSAGDIYKKTYTGTYCVGCEAYKTDKELEGGKCVLHPSLTPETISEENYFF
                     RFSKYQDKLLTYLSTEAVIVPEWRREEAINFVKNGLDDFSISREKARLSWGVPVPGDE
                     NQVMYVWFDALTSYISTLGWPTEGGNFEKFWQEGKVVQMAGKDQVRFQSLMWQAMLMS
                     ANIKNTDQVFYHGFINSGGQRMSKSLGNVINPYELVNKYGTDATRYLLLRHVHPFEDS
                     DVTWERLDEWYTANLVNGLGNLVARVMKLAEDNLSAPVALTEADTAVEEAFLEKLNAY
                     RFQEAMDLTFEHVGKGDEYMTATEPYKKVKVEDTKGEGILIIEKLVRHLAKLSAHLAP
                     IMPATAATILSAVKDNTKPENLFPRLAV"
     gene            complement(2213..3241)
                     /locus_tag="PK109_02990"
     CDS             complement(2213..3241)
                     /locus_tag="PK109_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRH24529.1"
                     /translation="MDKKHAITISFGGLIRIVIVAAAVWALWQLSTLVLLLLSAIVIA
                     SAAEPGVAFFLKYKFPRPLAVVSVYATVLACLIALVWFFVPPMLEEAIALLAVVPQYV
                     SQVSSLSALPFLQNASIGPSFTDTVLSLQNAFANTGAGVLRFVTSLFGGVFYFILTIV
                     VSIYFSLQETGVDDFLRLVTPAHQREYVLGLWKRSQKKIGLWMQGQLLLSLIITVLLF
                     LGLSLLGVPYALLLAIFAGIMELIPVFGSVAAAVPGIAVALTTGDLTLVALVSGLYLI
                     VNQFQAHLIYPLVVKKIVGVPPILVIIALVAGGQLAGVLGVLLSVPIAACVQEFVNDL
                     QKRRTVEA"
     gene            complement(3278..>3434)
                     /locus_tag="PK109_02995"
     CDS             complement(3278..>3434)
                     /locus_tag="PK109_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRH24530.1"
                     /translation="RQDEIGTPACITIDFDTLGEHPELLDTVTIRDRDTGQQERVAIS
                     ELSSRLS"
BASE COUNT          840 a          905 c          893 g          796 t
ORIGIN      
        1 cccttttgat ggacgtgcat gaatcatgag cggctttcct gtttgtatcg cgagctgaat
       61 gtgacgggca aacacatcct tctgacgtga cttctccgct tctggattct ctggccggaa
      121 ataatcgagt ccgcactccc ctatcgcaac gaccttcggg tctcgggcaa gttcttcgta
      181 gtacgcatca tcgtatcctt cttgcggttt atcgtttgga tgcagtccaa tagacgcgaa
      241 catagtcttt ccgtcggcaa gctgaacagc gcgtttagaa gtagtgctat cggtacccac
      301 aatgatgccg cccacttcgg cgtcctgcat tgcgcgtacg acttccgcgc ggtcagcgtc
      361 atatgcctcg aactgaatgt gacagtgcgc gtcaaaatat ttcatcacct gcaccatagc
      421 atattgaaaa gaggttacga catcgctgtc gtaacctctt ctattttatt ccggcttagc
      481 cgttgcaacc gcgacggctg caaaaaactt tccggcatgc actagtccag taagttctat
      541 tggtgcgcgt cgtgcttgtc gtgaatctgc aatagcctgc tcagcatcct tacggaccgc
      601 tttgcatgcc acaaattcct cagcaatgcg ctctagaact gttttttccg tagccattct
      661 cgtaacccct caaaggcaaa ggtattcgaa gactccacca tactgtgccg ttaaatataa
      721 tattacactt ttataatatt gtcaattacc taaatacggc acagtagagc catcattaga
      781 cagcaagacg tggaaagagg ttctctggct tcgtattgtc tttcacagca ctaagaatgg
      841 tagcggcagt tgccggcata atcggggcaa ggtgcgcaga aagcttcgct aagtgacgga
      901 ccagcttttc gataataaga atgccttcac ctttggtgtc ctctactttc actttcttat
      961 acggctcagt cgccgtcatg tactcatctc ccttccctac atgctcgaaa gtaagatcca
     1021 tcgcttcttg gaaacggtag gcgtttagtt tttctaggaa cgcttcctca acagcagtat
     1081 ctgcttcagt gagggcaacg ggtgccgaaa gattatcttc tgcaagcttc ataacgcgtg
     1141 caacgaggtt accgagtccg ttcactaggt ttgcggtata ccactcatca agacgctccc
     1201 aggtaacgtc tgagtcttca aagggatgca cgtgccgcag gagaaggtag cgcgttgcat
     1261 cagtgccgta tttattcacc agctcgtaag gattaatgac gtttccgagg gacttcgaca
     1321 tgcgctgtcc tcctgagttt atgaacccgt gatagaagac ctggtcggta tttttaatgt
     1381 ttgctgacat gagcatggcc tgccacataa gcgactggaa acgtacctga tcttttcctg
     1441 ccatctgcac caccttccct tcctgccaga acttttcgaa gtttccgcct tcggtgggcc
     1501 acccgagtgt tgagatgtaa ctcgtgagtg catcaaacca gacatacatg acttggttct
     1561 cgtcacctgg gaccggcacg ccccatgaaa gacgcgcttt ctcacgcgag atactgaagt
     1621 catccaatcc gttctttaca aaattgatag cttcttcgcg acgccactca ggaacgataa
     1681 ctgcttctgt tgaaagatac gtgagcagtt tgtcttgata ctttgagaac cggaagaagt
     1741 agttctcttc tgaaatagtt tcgggcgtaa gcgacggatg aagcacacac ttccctcctt
     1801 caagctcctt gtctgtctta tacgcctcgc acccgacgca gtaggtaccg gtatacgttt
     1861 tcttgtagat atcgcccgca gaatcgcacc ggcgccatat ttcttgcgct gctgcgtagt
     1921 gcgcaggatc ggtggtgcga ataaaggcat cataggaaag atcgagtgcg ctccccaact
     1981 tcttaaattc ccccgagaaa tgatcgacgt agtcatgcac gtctttccct gctttctctg
     2041 cagcctggag aatcttctgc ccatgctcat cggtaccggt gttgaagaag acttcgtctc
     2101 cctgtaggcg ccatgcgcga gcaagtgcgt ccgcctgtac aaactcaagc gcgttaccga
     2161 tatgcggatc ggcgtttacg tatggcagtg tggtcgtgag gtagcggcgc atttaagctt
     2221 caacggtcct tcgtttctgg aggtcgttca caaactcctg cacgcacgca gcgataggca
     2281 ctgagaggag tacgccaagg acacccgcaa gctggccacc ggccacaagc gcaatgatga
     2341 cgagaatggg cggcacgccc acgatcttct tcaccacgag cggatagata agatgcgcct
     2401 ggaactggtt cacgatgagg tagagaccgg acacaagtgc aacgagagtg aggtcaccgg
     2461 tggtgagcgc taccgctatg cccggtacgg cagcggcaac tgacccgaac accgggataa
     2521 gctccatgat gccggcgaag atagcgagga ggagtgcata cggcacgcca agcagtgaga
     2581 gaccgaggaa gagcaacacg gtgatgatga gggaaagaag aagctgtccc tgcatccaaa
     2641 gcccaatctt cttctgagaa cgcttccaaa ggccaagtac atactcacgc tggtgcgcag
     2701 gcgttacgag gcgtaggaag tcatccacac cggtctcctg aagggagaag taaatggaaa
     2761 cgacgatagt gaggatgaag tagaagaccc cgccgaaaag cgaagtaaca aagcgcagta
     2821 cgccggcacc cgtgttcgca aacgcgttct gaagcgaaag caccgtatca gtaaatgagg
     2881 ggcctatcga tgcgttctgg aggaatggga gcgcgcttag gcttgagacc tggctcacgt
     2941 actgcggcac caccgcaaga agcgctatcg cttcttcgag cattgggggc acgaagaacc
     3001 aaacgagcgc gataaggcat gcaagcaccg tggcgtacac ggacaccact gcgagcgggc
     3061 gcgggaactt gtacttcaaa aagaaagcca cgcccggctc agcagccgaa gcgataacga
     3121 tagcggacag gaggaggagc acgagggtcg agagctgcca gagagcccaa accgctgccg
     3181 caacgataac gatgcgtata aggccgccga acgagatggt gatggcgtgc ttcttgtcca
     3241 ttaggaagat actagcataa acgccctttt ggggcggtta gctgaggcga gagctgagct
     3301 cggagatagc aacacgttcc tgctgtccgg tatcacgatc tcgaatggta acggtgtcca
     3361 aaagctctgg atgttcaccg agcgtatcga agtcgatagt gatacacgcc ggggtaccta
     3421 tctcatcctg acgg
//
LOCUS       DAORIN010000070         3399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_6517_length_3399_cov_4.976974, whole genome shotgun sequence.
ACCESSION   DAORIN010000070 DAORIN010000000
VERSION     DAORIN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6517_length_3399_cov_4.976974"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK109_03000"
     CDS             <1..252
                     /locus_tag="PK109_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24531.1"
                     /translation="GGSRTTIGGLESTKQFKNGSAYVPLAATIGPKQFRIFTIKALVS
                     QYSGSYYGKQLKINVASVDSKASVTGIFPMLGTTWTLVY"
     gene            1269..1694
                     /locus_tag="PK109_03005"
     CDS             1269..1694
                     /locus_tag="PK109_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24532.1"
                     /translation="MEDFLNIEGKTYEFPAEGKVRMLDAAMLQAARSLAELEATTEQL
                     DRMLPRDVASEVVEPIKLASMNNLMEKMKSLVTQTGEQLASAADAAHGLVDNPALSEE
                     AQSATLQKLVDLNTRYDAVTKKMEVVGQRLKLPPPPPST"
     gene            complement(1684..2430)
                     /locus_tag="PK109_03010"
     CDS             complement(1684..2430)
                     /locus_tag="PK109_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRH24533.1"
                     /translation="MADEIKTTTELSEKMSTHLARLSGEGQVTVPENVEPLICKPRQL
                     EPWRVFKIMSEFVEGFELLQKYTLAATFFGSTRAALAPEIYQAATDLSAKLAKRGFAI
                     ITGGSAGIMEAANKGAYEAGGSSVGLNIKLDSAQPMNRYVTEQNFFDHFFVRKVMLTF
                     ASEVYVYFPGGFGTLDEFFEIVTLVQTKKIKQVPIVCYGKAFWNPLLEHFRKELKDTY
                     HTIDDGDMELFHVVDSVDEAFEYITKNVTC"
     gene            complement(2415..3011)
                     /locus_tag="PK109_03015"
     CDS             complement(2415..3011)
                     /locus_tag="PK109_03015"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRH24534.1"
                     /translation="MIVLDVETTGTNPDKHSILSIGALDLSNPTNQFYDECRMWDGAK
                     IEKDAIAINDFSEEDAQDPEKKSEAELIAAFFAWAEGVGQDRTLAAQNVSFDHAFLTA
                     AAARAGLDFPFAKRTLDIHSVAWLHMTIHGREVPIEHGHSGLNSTKVLAYCGLPEEAK
                     PHNALIGALWHAEVISRIAYTKKLLPEYEHFPIPWLTK"
     gene            complement(3033..>3399)
                     /locus_tag="PK109_03020"
     CDS             complement(3033..>3399)
                     /locus_tag="PK109_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HRH24535.1"
                     /translation="ASSASFGLGMLTERSMGPQDSGVKIEALQAGEALPAAAAIAPLP
                     KTTKSSTSKASTPKKVLPTGTATAETGKYVASRSGEKYYLPTCGTVNRIKEENKIYFN
                     TKEEAEAAGFEPSTACKGL"
BASE COUNT          829 a          882 c          842 g          846 t
ORIGIN      
        1 ggcggatctc gtacgaccat aggtggcctc gagtccacga aacaattcaa gaacggttct
       61 gcgtatgtgc cgctcgctgc cactatcgga ccaaagcaat ttcgcatctt taccatcaaa
      121 gcgttggtgt ctcagtacag cggcagctac tacggcaaac aactaaaaat aaatgtcgct
      181 tcggttgatt caaaagcttc cgttacggga atcttcccga tgctcggcac gacttggacc
      241 ttagtgtact agtattatca ctactaaaga aaccgccgat tcgactcggc ggtttctgct
      301 tatataagaa aaccgcttcc caaattacta ggaagcggcg gtacggcacg ggaaagttct
      361 gggcgttagt cgcaggtcat agttaggctg gggcccaacc tgcacgaggc ttgtcgcacc
      421 ctcccgtgcc gccgtatgag cccagggacg agaaaggctc accacgtcat actgagccta
      481 tgtgtttctc tcgtcaatat atctactccc ccgcgagata taaaacggcc cacttactac
      541 ttccttttac catctcggca tgacgagcca gataaagagg gaagtgcgac ttccccgatt
      601 ttgagccttt ttggggaagt cccttatacg actcattctt taagcctata tagaggggga
      661 ataccaggca aagctagaga gaaacgtcgt agagaggcaa atagatgcca tagaagttga
      721 gagaagcagg cagggtagga gaaaggatca gaagaccggc ccgcccctct ttgccctagg
      781 agtacttcgc aaaagatata ttgtgcgaag cttatctatt ctgagctggg caggaacaga
      841 accctaaaac tggactttaa aaattgcttc acttccctcc tttctactcc cgccaccaaa
      901 tattccggta aatattttct gtgacttttt ctgagtgata taaaaatatt cggaaaatga
      961 ccacttcccc gaaaacaggt ttttggcggt ggggagggat caaaatgaat cttttgtaag
     1021 tgcttaaagg ttaagtcccc tgccctacga aaaaggccgc acctggggag atgcggcctt
     1081 cataacgcgc tgttgaaaac agctgcccct tacgggtgtt cccaaaaagc tctcaacaat
     1141 ttccgttttc agccatggcc cgtcggggca caaatgactc tccagaacga catcgttttg
     1201 gtgcgacaaa tatgctatag aagctatatt ttgtcaatac ttctcttttt ctaccgtata
     1261 ctaggcatat ggaagatttt ctaaacattg agggcaagac gtatgagttc cccgcagaag
     1321 gaaaagtgcg aatgttggat gccgctatgc tccaggccgc tcgctcgctc gccgagcttg
     1381 aagcgacaac cgaacagcta gatcgaatgc ttccccgcga cgtcgcttct gaagttgtag
     1441 agcccataaa gcttgcgtcc atgaataatc ttatggaaaa gatgaaatct ctcgtcactc
     1501 agacgggaga gcaactcgcg tctgcagcgg acgcagcgca tggactcgtc gataaccctg
     1561 ccctttccga agaagcgcag tcggcaacgc tacagaaact agttgacctc aacacccgct
     1621 atgacgcggt aacaaagaag atggaggtgg tgggacagcg tttaaagctt cctcctcccc
     1681 cacctagcac gtaacgttct tcgtgatgta ctcaaacgcc tcgtctactg agtcgacgac
     1741 gtggaagagc tccatatctc catcgtctat cgtgtgatac gtgtccttca gttcttttct
     1801 gaagtgctcg agtaacggat tccagaatgc tttgccgtag cagacgatgg gtacctgctt
     1861 tatcttcttg gtttgtacga gcgtcactat ttcgaagaac tcatcaagcg taccgaagcc
     1921 gccggggaaa tagacataga cttctgaagc aaaggtcaac atcactttac gaacaaagaa
     1981 gtgatcaaag aagttctgtt ccgtaacgta gcggttcatt ggctgcgcgc tatcgagctt
     2041 gatgttgagc cctacggatg acccgcccgc ttcatatgct cctttgtttg ctgcttccat
     2101 gatgccagca gacccacccg taatgatggc aaagccacgc tttgcgagct ttgcagaaag
     2161 atccgttgct gcctggtaga tttcaggcgc aagggcggca cgagtggagc cgaagaaggt
     2221 tgctgcgagg gtgtatttct gaagaagctc aaagccttcc acgaactcgg acattatttt
     2281 aaagacgcgc cacggctcaa gctgacgagg tttacatatg agcggctcca cgttctcagg
     2341 caccgttacc tgaccctcgc cagaaagacg agcaagatgc gtactcatct tttcggaaag
     2401 ctcggtagtg gtctttattt cgtcagccat ggtatcggaa agtgctcata ctccggtaat
     2461 aatttcttag tatacgctat gcgtgagatg acttcggcgt gccagagcgc tccgatgagt
     2521 gcgttgtgcg gttttgcttc ttccgggagc ccacagtacg caagtacttt cgttgaatta
     2581 agtccggagt gtccgtgttc tatcggcact tccctcccgt gtatcgtcat atgcagccac
     2641 gctacgctat gaatatcgag cgtgcgcttg gcaaacggaa aatcgaggcc ggcacgcgcg
     2701 gcggcagcag tgagaaacgc atgatcaaag gatacatttt gcgcagcgag ggtacggtcc
     2761 tgccctaccc cttcggccca cgcgaaaaaa gcagcgatca gttctgcttc agatttcttc
     2821 tcaggatcct gcgcatcctc ttctgagaaa tcgtttatgg cgatagcgtc tttctcaatc
     2881 tttgctccat cccacatccg acattcgtca tagaactggt tcgtagggtt actgaggtca
     2941 agcgccccta tagaaagaat ggagtgttta tcagggttgg ttcctgtggt ttctacgtcg
     3001 agcacaatca tagctacaga ataccagaac cgttagaggc ctttacacgc agttgaaggt
     3061 tcaaaccccg cagcttccgc ttcctccttt gtattgaaat agatcttgtt ctcctccttt
     3121 atccgattca ccgtgccgca ggtgggtagg tagtatttct ctccgcttct ggacgccaca
     3181 tacttccccg tttcggcagt agcggtgccg gttggaagca ctttctttgg agtagatgcc
     3241 tttgaagtgc tcgatttggt cgtcttcggg aggggagcaa tggctgccgc agcgggaagc
     3301 gcctcccccg cctgaagcgc ctctatcttc accccgctat cctggggccc catagagcgc
     3361 tcggtgagca tcccaaggcc aaagctggct gaggaggct
//
LOCUS       DAORIN010000071         3365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_6637_length_3365_cov_5.992447, whole genome shotgun sequence.
ACCESSION   DAORIN010000071 DAORIN010000000
VERSION     DAORIN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6637_length_3365_cov_5.992447"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1063
                     /locus_tag="PK109_03025"
     CDS             212..1063
                     /locus_tag="PK109_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24536.1"
                     /translation="MDDKGQIRNIASLKKREDGVDYEQIVLEKGIFSNVFEKDIRRVY
                     IYKKSERIAKAIHMISPAFKDSKALRDRVQRVSIEIIDASILPPAESKDILARELLTL
                     GSVLRMARAAGILSPMNADIIMREAHNLLQEVSSYEEPKVALDEVPTLAALAKSVPSR
                     AEVSPMPIRPMRTSGTNTAPVSYKGQADKGHNGDKKDKNGRRDAILSILKSKGPSYIK
                     DLSILIREVSEKTIQRELQALVAEGKVTRTGERRWTTYTLAGAGTFQPSPITVGEASV
                     DFAGSEG"
     gene            1614..>3365
                     /locus_tag="PK109_03030"
     CDS             1614..>3365
                     /locus_tag="PK109_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRH24537.1"
                     /translation="MVATTNAIAKIAAVVAGLGLVATSFAAFAAPAKAATTDELQAQI
                     TALLAQIAALQGGSSASASATFTMDLTLGSSGAEVTALQNWLISKGYSIPAGATGYFG
                     AQTQSALAAWQAANGVTPAAGYFGPITRAKVNAMAGGSTGGNTGNTGGLSGGEASLED
                     LQSRDGADDEVELGGTAEVAEFEVDVKDGDIELSRLDLTFDSSAVGTGDDDEPWDVFG
                     EVTISVDGKEVASEDVSDEDDWLDNDGTNGEYVFRFTGLDAVVREDDSAIITVSVEAD
                     GGADLGTDDQATWELYIDTDGLRGTDGEGIEQYAGDDSENVTFDVVEEGEDDALDIRR
                     SSTDPDSTTLKVEDDKKSDWYTIFAFDLEADEDGGDIELNTIPVVFTTSATTSLQNDI
                     INDAKIVIDGEEFDDFDWGTGGNASTTYFDVDGDFTVDAGETVTVEVMVEFRSQVGNY
                     VTGSTISAAATGTGFDAEGADDVTVGGSATGDTHTLQLEGIDVETNDGDNYIADATSV
                     DGGNDYGEFAVVVDITAFESDVFISATSSAFTYQIEDSAGAATTTLTGTYSATVSSDA
                     DVDSGNYVIDEGSTEEFT"
BASE COUNT          825 a          848 c          893 g          799 t
ORIGIN      
        1 tgctccttct ttgatttctt gcccttcatg tcatgaattt tagcacctac ctgcctatac
       61 gtgtgtattt aatgagggcg taggtacggc atttatttaa ggtttcatag cacgtggcgt
      121 gcgataaaaa atacgacata accgacattt gaaaaaactt gcgtgacctt ttgtgcggag
      181 tatacttgtc cttgaccgat acgaaattta tatggacgat aaaggacaaa taagaaacat
      241 tgcgtccctt aaaaaaaggg aagacggtgt tgattatgaa caaatagtcc tagaaaaagg
      301 tatattctcg aacgtattcg aaaaggacat acgtagggtc tacatctaca agaagtcaga
      361 acgtatcgca aaagcgatcc atatgatctc tcccgccttc aaggactcaa aggcacttcg
      421 ggaccgtgtg cagcgcgtgt ctattgaaat aattgatgcg tcgatactcc ctccggctga
      481 gtcgaaagac attcttgcgc gtgaacttct tacgttagga agcgtacttc gcatggcgcg
      541 agcggcgggt atcttgtcac ccatgaatgc cgatatcatt atgcgtgaag cgcataatct
      601 tctccaggaa gtatcgtcat atgaggaacc aaaagtagcg cttgatgagg tgccgaccct
      661 tgcggcgctc gctaagagcg tgccatcacg ggcagaagtt tctccaatgc cgatccgccc
      721 catgcggact tctggaacaa atactgcgcc cgtctcatat aaaggacaag ctgataaagg
      781 acataatggt gataaaaagg acaagaacgg acgccgtgat gctatcctct ctatcctaaa
      841 atcaaaaggt ccttcctata taaaggacct ctctatcctt attagagagg taagtgagaa
      901 gaccattcaa cgtgagctcc aggcactcgt tgctgaaggg aaggtgaccc ggactgggga
      961 gcgccgctgg acgacctata cgcttgccgg ggcaggaacc ttccaaccgt cccctataac
     1021 ggtaggggag gcctccgttg actttgcggg ttctgaggga tagtatctct gggtgggagc
     1081 ccctcggaaa taagtgagaa agcctaggcc ggaaacggcc tatttccatt tataccgtcg
     1141 gtgttatcca cacgataaaa acggccttag tactccctct atcggtatac ttcactgtat
     1201 accgctacgc agggggctta ttggctgaac gactgtttca ttgacatgct tcatcgggcg
     1261 ctcattcttc tctggctata gtgccggcgg aaagcaggct cccgatgata agaggtttgt
     1321 catgtgtgtc acacggtcat ataccgcgtg aatactcgtc ttctcgctag gaggagggag
     1381 tatcagaacg gatgcaattt tctgattacg gatggatgga agataaggaa acaaactttg
     1441 ccagctagcc gatgggggat gggtcgatac tgtcctctgt cggattagcc tgaaaatgtg
     1501 tgaaagcgtc gcgcaagcga cacacaatta gtaagaactg aaacgatgcg catgcggcgc
     1561 acaattatta gccgcgtcgc agcgcttcga ataacaaatt aaaaaacgaa tttatggttg
     1621 caactacaaa tgcaattgca aagatcgcgg cagttgtcgc tggtctcggc ctcgtcgcca
     1681 cctcgtttgc agctttcgcc gctccggcaa aggcagctac gactgacgaa ctccaggctc
     1741 agatcactgc gcttctcgct cagatcgctg cacttcaggg cggttcttcc gcttcagctt
     1801 cggctacctt tacgatggac cttacgctcg gatcttccgg cgcagaagtc accgcacttc
     1861 agaactggct catctcaaag ggttactcaa tcccagcagg tgctactggc tacttcggtg
     1921 cccagacgca gtctgctctt gccgcatggc aggcagctaa cggagttact ccagcagctg
     1981 gttacttcgg tcctatcacc cgcgccaagg ttaacgctat ggcaggtggt tctaccggtg
     2041 gaaacacggg taacaccggc ggtctctcgg gcggcgaggc ttctcttgaa gacctccagt
     2101 ctcgcgatgg cgctgacgac gaggtagaac tcggcggtac ggctgaggtc gcagaattcg
     2161 aagtcgacgt caaggacggc gacatcgaac tctcacgcct tgacctcacc ttcgactctt
     2221 ctgcagtcgg tactggtgat gatgatgagc cttgggatgt cttcggcgaa gtcaccatct
     2281 ctgttgacgg caaggaagtc gcttctgagg atgtttccga cgaagatgac tggcttgaca
     2341 acgatggaac gaacggcgag tacgtcttcc gcttcaccgg ccttgatgcc gttgtacgcg
     2401 aagatgactc tgcaatcatc acggtctcag ttgaggctga tggcggtgcc gacctcggta
     2461 ctgacgatca ggctacctgg gagctctaca tcgacactga cggccttcgt ggcacggacg
     2521 gtgaaggtat cgagcagtac gccggtgatg attctgagaa cgtaaccttc gacgttgttg
     2581 aggaaggtga ggacgacgca cttgatattc gtcgctccag cactgaccca gattccacga
     2641 cccttaaggt tgaggatgac aagaagtctg actggtacac gatcttcgca ttcgatcttg
     2701 aagcagatga ggacggtggc gacattgagc tcaacaccat ccctgtcgtc ttcacgacga
     2761 gcgcaacgac gagcctccag aacgacatca tcaatgacgc aaagatcgtt attgatggcg
     2821 aagaattcga tgacttcgat tggggtactg gcggcaacgc aagcaccacc tacttcgatg
     2881 ttgacggtga cttcactgtt gatgcaggcg agactgtaac tgttgaagtt atggttgagt
     2941 tccgcagcca ggttggaaac tacgtaaccg gttcgaccat ctcagcagca gctaccggca
     3001 ccggattcga tgctgaaggc gctgacgatg tcacggttgg tggttctgca acgggcgaca
     3061 cccacacgct ccagcttgag ggtatcgatg ttgagaccaa cgatggtgac aactacattg
     3121 ctgatgcaac ttcggttgac ggcggcaatg actacggaga gtttgcagtt gttgttgata
     3181 tcacggcctt cgagtctgat gtcttcatct cagctaccag ctctgccttc acctaccaga
     3241 tcgaggattc ggctggcgca gctacgacca cccttacggg tacgtacagc gcaaccgtct
     3301 catctgacgc agatgtcgat tccggcaact acgtaattga tgagggctcg acggaggagt
     3361 tcacc
//
LOCUS       DAORIN010000072         3321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_6805_length_3321_cov_4.075321, whole genome shotgun sequence.
ACCESSION   DAORIN010000072 DAORIN010000000
VERSION     DAORIN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6805_length_3321_cov_4.075321"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..216)
                     /locus_tag="PK109_03035"
     tRNA            complement(146..216)
                     /locus_tag="PK109_03035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Gln,seq:ctg)
     gene            408..1082
                     /locus_tag="PK109_03040"
     CDS             408..1082
                     /locus_tag="PK109_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRH24538.1"
                     /translation="MRLLVVEDEPKLASVIAKGLEAKGYAVDTIGDGGEALTRILVHG
                     KDYDLVVLDLMLPTKTGAEICKETRAAGITVPILILTARSETEHKVELLLSGADDYMV
                     KPFSFEELIARIQAILRRPTAALSTVLVGGDIELDPSTHTVKQDGKDVSLTLKEFSLL
                     EYFLRHPNEVVNREDLLTHLWDFNYESFSNVVDVHIKNLRQKLDKKDAESILETIRGI
                     GYRLRV"
     gene            1027..2148
                     /locus_tag="PK109_03045"
     CDS             1027..2148
                     /locus_tag="PK109_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRH24539.1"
                     /translation="MLKAFWKRFAESVIGFAYKYRHDVFYRTGWNVVVLQVGFASVLL
                     VIVVASLNLLYDEVIAALVHSIASSLATGSSPTLDSISIASSLEYEKQKSMVLTATGV
                     LVAAAIFSWLITRMALTPTRDALDSQKQFVGNIAHELRTPLSIIKTNTEVALFNERLD
                     TDTAEMLRSNVEELDRISHIINNLLSMSALLRPEKMEFVSVDAGAIIDRVIDLLSDLS
                     IRKGVALEIHKGMERPVWGNAAALEQILMNLVKNAVNYTPAGGHVRITTDVDLGGRVE
                     VRIEDSGIGISEKDLAHVFEPFYRGDQSRNRKSGGTGLGLAIVSELVKVHKGTISVRS
                     IVDQGTTVILSFPAAKTSTDHPAFAVDFSNPFAKKPGTS"
     regulatory      2374..2474
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2535..>3321
                     /gene="metK"
                     /locus_tag="PK109_03050"
     CDS             2535..>3321
                     /gene="metK"
                     /locus_tag="PK109_03050"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRH24540.1"
                     /translation="MFHLLTHYTTESVTAGHPDKVCDQMSDAILDECLKQDPKSRVAV
                     ETFGSHGTLMIGGEVTTNAQVDYAAVARSVYEDIGYTEPLEILSKVVVQSPDIAMGVD
                     TGGAGDQGIMYGYATDETKEMLPKGVVLAHALARKLEALRRDGILPYLRPDGKTQVTL
                     KEGNVITALTSTQHAEEAELEQIRADLTEHLFPAVLGDVSGIEILVNPTGRFVQGGFE
                     ADAGLTGRKIMVDSYGGLMPHGGGAFSGKDSTKVDRSAAYMCRF"
BASE COUNT          789 a          911 c          871 g          750 t
ORIGIN      
        1 gtgccattca ctccttcttc ggcgacgagc acgcggcccg tcagttcata gaacgaagcg
       61 cgctccaaaa accacgcacg gagtgcctgt ggatcttcga tggtgacgta tttataaaaa
      121 aggagaactt gatattccat aaatgctgcc agactaggat tcgaacctag ataaacggat
      181 tcagagtccg ttgtcctacc attagacgat ctggcaatga cacaagcggc gtacgccgcc
      241 cggttctggc caccatagcg caaaatggcc tctgtttcaa tgactttttg ctccccctct
      301 ccctcatggg atagtatgaa gagtcttcat gaagccttca taggagcttt gcgaagatgg
      361 ccggtatcgc cctcgtgcga cctttaccct cctaactccc ctcacctatg cgtctattgg
      421 tagttgaaga cgaaccgaag cttgcgagcg ttatcgccaa aggcctcgaa gccaaaggct
      481 acgccgtcga cacgattggc gacggcggcg aagcgctgac ccgcatcctc gtgcatggca
      541 aggactatga ccttgtagtg ctcgacctca tgcttcctac gaagacgggt gcagaaatct
      601 gcaaagaaac ccgtgcggca ggaatcacag tgcctatcct catccttacc gcacgaagcg
      661 agaccgagca taaagtggaa ctacttctct cgggagctga cgattacatg gtgaagccgt
      721 tctcgtttga agaactcatc gctcgtattc aggcgatact gcgtcgcccc accgccgccc
      781 tctccaccgt actcgtgggg ggcgacatag agctcgatcc ctcaacccac actgtgaagc
      841 aggacgggaa agatgtttcg ctcacgctta aagagttcag cttgcttgag tacttcttgc
      901 gtcacccaaa cgaagtggtg aatcgcgagg atctactcac ccacctctgg gacttcaatt
      961 acgaatcatt tagtaacgtg gtcgatgtgc atataaagaa cctccgacaa aagttggaca
     1021 aaaaagatgc tgaaagcatt ctggaaacga ttcgcggaat cggttatcgg cttcgcgtat
     1081 aaataccgac acgacgtctt ctaccgcact gggtggaatg tcgttgtgct ccaggtaggc
     1141 tttgcgagcg tactgctcgt catagtcgtc gcctcactga atctcctcta tgacgaggtg
     1201 attgcggcac ttgtgcactc gatagcatcg agcctcgcca ccggatcaag tccaactttg
     1261 gacagtattt ctatcgcctc aagtcttgag tacgagaaac agaagagtat ggtacttacc
     1321 gctaccggcg tactcgtcgc tgccgctatc ttcagctggc tcatcacccg tatggcgctc
     1381 accccaacgc gggatgcgct tgatagccag aaacagttcg tcggtaacat cgctcatgag
     1441 ctgcgcactc cgctttccat tatcaagacg aatactgaag tcgcgctctt taacgagcga
     1501 ctcgatactg acaccgccga gatgctccga agtaatgtag aggagctgga tcgcatctcc
     1561 catatcatca ataacctgct ctccatgagc gcgttgctcc gccctgagaa gatggagttc
     1621 gtaagcgttg acgcgggggc gatcattgat cgcgtcatcg acttattgtc agacctctct
     1681 attcgtaaag gagttgcact cgaaatacat aaaggtatgg agcggcctgt gtggggaaat
     1741 gcagcggcac ttgaacaaat actgatgaac cttgtgaaaa atgccgtgaa ttacacgcct
     1801 gccggcggcc acgtacgcat tacaacggac gttgatctgg gtggacgcgt agaagttcgc
     1861 attgaggata gcggtatcgg gattagtgaa aaagaccttg ctcatgtctt cgaacctttc
     1921 tatcgcggcg atcaatcacg caaccgcaag agcggcggca ccgggcttgg gcttgcgatt
     1981 gtgtctgagc tcgtcaaagt acataaaggc accattagcg ttcgcagcat cgtcgaccag
     2041 ggcactaccg ttattctttc ttttcctgcg gcaaagacaa gtacagatca tccggcgttt
     2101 gcggttgatt tctcaaaccc attcgctaaa aaaccaggta cttcataatc gcttcacgac
     2161 agttgcatga gaatagtgga gcgcgcacta atcagccgtg cgaaccattt tagcgcgtat
     2221 gttctttcgg actcagcacg cacctgggtc ctgcatatgg tgggcggctc ccactatacg
     2281 agaggccgtc tcaggccgcc ctcgaagggc ggcctgtcgg cgtacgggga ttgcaggagc
     2341 ggcgcctcat gctagagttt ccgtacaact gaatagatat acagaagcgg ggagagcact
     2401 ggctccatga accgctagca accagatcac atgatcacgg tgctccctcc agcccttcgc
     2461 ggggaatata ttcgtaagaa catagtcccg tgaagcggga ctatttcttt tgtatttact
     2521 cttcacttat ttctatgttc catttgctca cgcattacac gactgaatcg gttacggcag
     2581 gacacccgga caaggtctgc gatcagatgt ctgacgccat ccttgatgag tgccttaagc
     2641 aggacccaaa gtcacgcgtc gcggttgaaa ccttcggcag ccacggcacg cttatgatcg
     2701 gcggcgaagt caccacgaac gcgcaggtgg actacgcagc ggttgcgcgc tcagtgtatg
     2761 aagacatcgg ctacaccgaa ccgcttgaga ttctctcgaa ggtggtcgtg cagtctcctg
     2821 acattgctat gggcgttgat accggtggcg cgggcgacca gggcatcatg tatggctacg
     2881 caacggatga gaccaaagaa atgcttccaa agggagtcgt gcttgcgcac gcgctcgctc
     2941 gcaagctcga agcacttcgc cgagacggca tccttcccta ccttcgccct gacggcaaaa
     3001 cccaggtgac gttgaaagaa ggtaacgtta tcactgcgct cacctcaacc cagcacgccg
     3061 aagaagcgga attggagcag attcgtgctg accttacgga gcacctcttc ccggcagtac
     3121 tgggggatgt ctcaggtatc gagatacttg tgaatccaac gggacgcttc gtgcagggtg
     3181 ggtttgaagc agacgccggc ctcacgggtc gcaagatcat ggtggatagc tacggcggcc
     3241 ttatgccgca cggtggtggc gcgttctcag gaaaagactc caccaaggtg gaccgctccg
     3301 cagcatacat gtgccgcttt g
//
LOCUS       DAORIN010000073         3290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_6916_length_3290_cov_5.571252, whole genome shotgun sequence.
ACCESSION   DAORIN010000073 DAORIN010000000
VERSION     DAORIN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6916_length_3290_cov_5.571252"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PK109_03055"
     rRNA            <1..733
                     /locus_tag="PK109_03055"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            821..926
                     /gene="rrf"
                     /locus_tag="PK109_03060"
     rRNA            821..926
                     /gene="rrf"
                     /locus_tag="PK109_03060"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1025..1246
                     /locus_tag="PK109_03065"
     CDS             1025..1246
                     /locus_tag="PK109_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24541.1"
                     /translation="MPGARLPDLRPGEPPFQVDDEDHRKLALKMVGDAIGQAEASLSG
                     SQNPVDTQGGIRYLEQQRQAIINAAVRTA"
     gene            1311..2078
                     /locus_tag="PK109_03070"
     CDS             1311..2078
                     /locus_tag="PK109_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24542.1"
                     /translation="MSWAAERRTLILAIVGVVILAIASFFAYQFFHQAPSCTDGKQNQ
                     EEEGIDCGGTCTYLCAAKLVAPSVSFARELPQNGGRTDVIAYIKNPNPSAAAKGVAYT
                     VELYNAERAVVATKKGTVDLAPALETPVYIPNFNSGNQEVDRVFISIDNTLYRWFRFT
                     EEPKVPTVASPQVSGTEAAPRVTAVVTNPFPTALKNIKLVATVFDGEGNAIAASQTVV
                     PTLIPQGRADAVFTWNAPFPGIVSRIDVKPIVPLPAP"
     gene            2075..3211
                     /locus_tag="PK109_03075"
     CDS             2075..3211
                     /locus_tag="PK109_03075"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRH24543.1"
                     /translation="MSELVRSGSFRALLSSWPLLLSAVALSLLGVLTMHPFGGDSSLA
                     PRQLLWIGVGVLVYAACAMADMHIIRRTTVLVAGYVLVLGLLVLLLLVAHPVLGAKAW
                     FDLGPFSFQPADLAKLVLIAFLAKYFSRRHQEIKHIQHLILSGVYTAVIIGLILVQPD
                     LGTAAILSIVWFGMVLVSGISKKHLAIVFILGIVTAAGLWFFGLHDYQRTRIVAFLNP
                     AADIRGAGYNAYQAVVAAGSGEWFGKGIGYGTQSKLRFLPEYETDFIFAAFAEEWGFV
                     GVVLVLLLYGLLLFNLLSIAQRSATNFDAFFTIGIALLMLAHVFIHAGINLGLLPVTG
                     TTIPFMSYGGSHIVMEFAALGIVASLARHGRSAPRTMAGQEYEG"
BASE COUNT          729 a          860 c          869 g          832 t
ORIGIN      
        1 tcggcacctc gatgtcggct caccttagcg cggggctgga gaaggtccca agcgtttggc
       61 tgttcgccaa ttaaaaaggt acgcgagctg ggttcagacc gtgagggttg ttgatactcc
      121 tgtatcgacg ctctcaccgt ctgaatccca ttgaagtaaa ttgaaaaaga actgttatcg
      181 gatccctaat gaaggttttt tcgtctacaa atgattctgt cgtgcacccg gtggggtgcc
      241 cgaacaatgg aaaccaacaa tgacacggcg gtcatgcata gggatatgca tgtatcaaac
      301 tgactcatat cggaaaagtc ccacgtggat aattccgagg gaaccgtaag ggcccgtaga
      361 gactaagcgt cagttgcgaa gaactcgcaa agcaatagtc cgatcctttc agcaatggaa
      421 gtatataaaa aacgaaatgc gtgagacagg ttggtctcct atctactaca ggcgttgatt
      481 tttgaggagg cttgccccta gtacgagagg accggggtga actgacctct ggtctaccag
      541 ctgtcgtgcc aacggcaccg ctgggtagct atgtcgggtt gagataactg ctgaaagcat
      601 ataagcggga agctcgctcc aagattagaa atcgtatgag actcctagca gactactagg
      661 ttgataggct ctagatgtac ggccagtgat ggcttcagtc gaggagtact aatatgtcca
      721 gttttctgtg cggaattatg tgcgccacag cttcttgctg tacttcatac cttttgcgta
      781 ttcgtttgtc cgaggtcgtt tatagaaacg agcccggaac gctggtgatt tgtcagagag
      841 gccatacccg ttcccattcc gaacacggca gtcaagctct cgagaaccga tggtactcga
      901 aaggggagag taggtagtcg ccagcgttct gggctcgttt catttgcaaa agccgtccga
      961 aagggcggct tttgctattt catggtatga aaggcagggg aatagttccc aaggagacac
     1021 catcatgcca ggagctcgac ttcctgacct gcgaccaggc gaacctccat tccaagtaga
     1081 tgatgaagat catcggaaat tagccctcaa gatggtgggt gacgccatcg gtcaggcaga
     1141 agcatctctt tctggttccc agaatcctgt tgatacccaa ggaggaattc ggtacctcga
     1201 gcagcaacga caggcgatta ttaacgccgc cgttcgcacc gcatgattgc ttcggcaatt
     1261 gtgataaaag ccgtcccggc aacgagacgg cttttgctat acttaccgct atgtcctggg
     1321 ccgcagaacg aagaacgctc atcctcgcta ttgttggcgt ggtgatacta gctatcgctt
     1381 cattctttgc ctatcaattc tttcatcagg caccatcgtg cactgacgga aaacagaatc
     1441 aagaagaaga gggaatagac tgcggtggca cctgcaccta cctctgtgcg gcaaagctcg
     1501 ttgcaccctc agtatcgttt gcgcgtgagt tgccgcagaa tggaggacgc acggacgtca
     1561 ttgcgtacat caagaacccg aatccttctg ctgccgcaaa aggcgttgcc tacacggtgg
     1621 agttgtataa cgcagagcgc gcggtggtgg caacaaagaa gggaacggtt gatctggcgc
     1681 cggcgcttga gacgccggtc tatatcccaa actttaattc aggaaatcag gaagtggatc
     1741 gcgtattcat ttctatagac aacacgttgt atcgttggtt ccgctttacc gaagaaccaa
     1801 aagttcccac cgtcgcaagt ccgcaggtga gcggcaccga agcggcgccc cgcgttactg
     1861 ccgtcgtcac caatcccttt ccgacagcgc ttaagaacat aaagctcgtc gctactgtat
     1921 tcgatggaga aggtaacgct atcgctgcct cgcaaaccgt cgtgcccacg cttatcccgc
     1981 aaggccgcgc cgatgctgtt ttcacttgga acgcaccgtt tccaggcatc gtctcgcgca
     2041 ttgatgtgaa gcctatcgtg ccgcttcctg ctccatgagt gaactagtgc ggtcaggtag
     2101 tttccgggcg ctcctctctt cgtggccgct ccttctctcg gcggtagcac tctcgctcct
     2161 tggcgtcctc accatgcatc cgtttggtgg tgatagttcg cttgccccac gtcagctgct
     2221 ttggatcggg gtgggagtcc tcgtgtatgc cgcgtgcgcg atggcagata tgcatatcat
     2281 ccgtcgcacc accgtgctcg tcgcagggta tgtactcgtc cttggactcc tcgtgctcct
     2341 tttactagtg gcacaccccg tccttggcgc gaaagcatgg tttgatcttg gtcccttctc
     2401 ctttcagccg gcagacttag caaagcttgt gctcatcgcg tttctcgcca aatacttctc
     2461 gcggcgtcac caagaaatca aacatataca gcaccttatt ctctcgggcg tctacaccgc
     2521 ggtcatcatc gggcttattc tcgtgcagcc agaccttggt actgccgcaa ttctttctat
     2581 cgtgtggttt ggcatggtgc tcgtctcggg catttcaaaa aaacatctcg ctattgtctt
     2641 tattcttggc atcgtgaccg ctgcaggact ctggttcttc ggtcttcatg actaccagcg
     2701 cacgcgtatc gtcgccttct tgaatccggc agctgatatt cgtggtgcgg gatataacgc
     2761 gtatcaggcg gtagtcgctg cgggttcagg ggagtggttc gggaaaggaa tagggtacgg
     2821 gacgcaatca aagctccggt tccttcctga gtacgagacg gactttatct ttgccgcgtt
     2881 tgcggaagag tggggctttg ttggggtcgt gctcgttctt ctcttatacg gactccttct
     2941 ctttaacctc ctttcaattg cacagcgatc tgcgactaac tttgatgcct tctttacgat
     3001 aggtatcgcg ctccttatgc ttgcgcacgt attcatacat gccggtatta accttgggct
     3061 ccttccggtt actggtacca ccattccgtt catgagctat ggcggctccc atatcgttat
     3121 ggagtttgca gcgctcggca tcgtcgcctc cttggcgcgt cacgggagaa gcgcaccacg
     3181 cacgatggcg gggcaggagt acgaaggata acaatagtcg aaattgacat tcgatgtgct
     3241 tcgtttagga tgcgcctacc caatcacgac agaaaagaaa gatagtgaat
//
LOCUS       DAORIN010000074         3206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_7267_length_3206_cov_4.270073, whole genome shotgun sequence.
ACCESSION   DAORIN010000074 DAORIN010000000
VERSION     DAORIN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7267_length_3206_cov_4.270073"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..620
                     /locus_tag="PK109_03080"
     CDS             135..620
                     /locus_tag="PK109_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRH24544.1"
                     /translation="MTKKNASKGFTLIEILIVIGIIAILAAIVIVAINPAKQFAQARN
                     TQRESNINTILNAVGQNIADHKGVFSCNGEEADISGSPSPIGNGTPDGTYVDLEDADC
                     LVPTYMPSMVFDPIGDAVDTLYTIEVDGNGRYTVCAPEGATPNEPALGDLEPATLCVT
                     R"
     gene            complement(668..1381)
                     /locus_tag="PK109_03085"
     CDS             complement(668..1381)
                     /locus_tag="PK109_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRH24545.1"
                     /translation="MTVLVHIIGILILSIVAFWGANTFLKARISKALMEHEEPYMNET
                     LDQRKTLLVLGDSTGVGVGADKPEHSVAGRLAAYLGATYVENHAVSGALTEELSFQIQ
                     EAKLPKYDYILLQIGGNDILAFHSAKKTAAQLSTALGKLPPAGQIIIMSAGNAGGGTL
                     FPPPLRPFHTLVNLAVHKEFARVAHGWRATYVNLFDPFWKDPFLRNPYRYLSRDGLHP
                     SSYGYGLWFEKVKEALEKE"
     gene            1514..1873
                     /locus_tag="PK109_03090"
     CDS             1514..1873
                     /locus_tag="PK109_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24546.1"
                     /translation="MSDFKDLQLKTRDARPGEALEINYVGPHGVRGFTALGDRSDDPE
                     VVCIPQGAQLLLSNIPPYLQQRWGIHDTISAVADLRAPEAAKGFFTDGVRIEGRDDHI
                     SLQDLPEHLVATVRALA"
     gene            complement(1930..3138)
                     /locus_tag="PK109_03095"
     CDS             complement(1930..3138)
                     /locus_tag="PK109_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRH24547.1"
                     /translation="MQKKVLPILFLTLLLDMIGTGMVFPIIPIIFTDATSPHFLLQGY
                     STGMQFFIAGLITALFGLMQFIAAPILGELSDVYGRKKLLTLGVGVLAVSQLLFGFGI
                     AAKLVWLLLIARAIAGLAAANFSIAQATIADISSPEDRAKNFGLIGAAFGIGFILGPL
                     LSGWIAGVTGDAAAPFWFAGALGIVNLIFISLFLPETNHNRKAEHNFHIFKGIQNIKA
                     AFDDKDARPVYLSSFLYLSGFSFFTSFVGILLVSNFGQDEAGVGTYFGIVGACIVVTQ
                     LFILRFLTKHYKERQILRYTMLAAAFILFLHPFAPNALWLFALIPFFAVPQALTMANM
                     TALVSKSVSPQKQGAALGINSSLIALAQGVVPLIAGGGAGIIGVHAPFVAAALFMIGA
                     WWILFGPGHR"
BASE COUNT          825 a          841 c          858 g          682 t
ORIGIN      
        1 cccctccccc acccgctatc gtcatcggcg catggctcga actcccatga tctcttaccc
       61 attgactttc accgatatgt aatctataac ggcggaagtc tgttctcgtt ccattattta
      121 tctattcaat tcacatgacc aaaaagaacg cttcgaaggg cttcacgctc atcgaaatcc
      181 tcatcgtcat cggcatcatc gcgattctcg cggcgattgt cattgtcgct attaacccag
      241 ccaagcagtt tgctcaggct cgcaacacgc agcgcgaatc gaatatcaac accattctca
      301 atgcggttgg acagaacatt gctgaccata agggtgtttt cagctgtaac ggcgaagagg
      361 ctgatatcag cggtagtccg tcacctatcg gaaatggtac tccggacgga acttatgttg
      421 accttgaaga tgcagattgc ctcgtcccaa cgtacatgcc atcgatggtc tttgacccga
      481 ttggcgatgc agtagacacc ctctacacta tcgaagtaga tggtaacggt cgttacacgg
      541 tgtgcgcccc tgaaggagcg accccgaacg agccagccct tggcgacctc gagccagcta
      601 cgctctgcgt cactcgctaa gcattctttg cttaaataaa aagccccgct tctgcggggc
      661 tttttgttta ttccttctct aacgcctctt tcactttctc aaaccagagt ccgtacccat
      721 aacttgaagg atgcagtccg tcgcgcgaaa ggtagcgata agggttacga aggaagggat
      781 ctttccagaa cgggtcaaag agattcacgt aggtggcgcg ccagccgtgc gcgacgcgtg
      841 cgaattcttt atgtaccgca agattcacga gcgtgtggaa cgggcgcagt ggtggcggga
      901 ataacgttcc tcctccggcg ttcccagcag acatgatgat gatctgccct gcaggtggaa
      961 gctttccgag cgctgtagag agttgcgcag cggttttctt cgcagaatga aacgcgagta
     1021 tgtcgtttcc gcctatctgc agcaaaatgt agtcatactt tggaagcttg gcctcctgta
     1081 tttgaaacga aagctcttcg gtgagcgcac ccgacaccgc atggttttct acataggtcg
     1141 cgccgagata cgcagcaaga cgcccggcaa ctgaatgctc tggcttatcc gctcccaccc
     1201 ctaccccggt ggagtcgccg agtacgagca gagtcttacg ctgatcaagc gtctcgttca
     1261 tgtacggttc ctcatgctcc atgagtgctt tagagattct cgctttaagg aaggtgttcg
     1321 caccccagaa ggcaacaata gagagaatga ggataccaat gatgtgcacc aaaacggtca
     1381 tacgcttagc ctaccgtgta atggagaaaa gaaaagttat cggtcgataa cgatagtggc
     1441 gtacgaagaa tgtttctgtt agcttcttaa gaactaacgt gcaatttcgc cgttagcgga
     1501 aaagaggttt ccgatgagcg atttcaaaga cttgcaactc aagacgcgcg acgcgcggcc
     1561 tggagaagca ctcgaaatca attatgtcgg gccgcatggc gttcgaggct tcactgctct
     1621 cggcgaccgg tcggacgacc cggaagtagt atgtatccct caaggagcgc agctgcttct
     1681 ctcgaacatt cccccgtatc tgcaacagcg ctggggtata cacgatacga tttctgcagt
     1741 cgctgacctg cgagcgccag aagccgccaa gggcttcttt acggacggtg tccgcatcga
     1801 agggcgtgat gaccatatca gtctgcagga tctgcctgaa catctggtcg ccacggtgcg
     1861 cgcactcgcg tagcgcatct gacgaagaaa taaatggggc gggtagctta ggctatccgc
     1921 tcctttcatt taccgatgac cgggaccaaa gagtatccac cacgcaccga tcataaagag
     1981 tgctgcggca acaaagggag cgtggacgcc gataatgccg gcccctcctc ctgcaataag
     2041 gggtacgacg ccctgtgcaa gcgcgataag cgaactatta ataccgagcg ccgctccctg
     2101 cttttgcgga gagactgatt ttgaaacgag cgcggtcata tttgccattg tgagcgcctg
     2161 cggcaccgcg aagaacggga tgagcgcgaa gagccagagt gcgtttggag caaacggatg
     2221 caagaagaga atgaaggctg ccgcaagcat ggtgtagcga agaatctgcc gctccttgta
     2281 gtgcttggtg aggaagcgaa ggataaagag ctgggtcacg acaatacagg cgccgacaat
     2341 accgaaatac gtccctactc ctgcttcgtc ttgcccgaag ttcgacacga gaagaatacc
     2401 gacgaaggaa gtaaagaagg agaacccgga gagatagagg aagcttgaga gatataccgg
     2461 acgtgcatcc ttatcatcaa acgctgcctt gatgttctgt atacccttaa agatatggaa
     2521 gttgtgctca gcctttcgat tgtggttcgt ttcaggaagg aagagcgaga tgaagataag
     2581 gttcacgatg ccgagtgcac ccgcaaacca gaacggcgcc gcggcgtctc ccgtaacccc
     2641 ggcaatccat ccggagagaa ggggcccgag aataaagcca ataccaaacg ctgcaccgat
     2701 gagaccgaag ttcttcgctc gatcttcagg agaagagatg tcggcgatag tggcctgcgc
     2761 tatggagaag ttcgcagcag caagtcctgc tatggcgcgt gcgataagca ggagccatac
     2821 gagctttgcc gcaataccaa aaccaaacag aagctgcgat accgccaaca cccctacccc
     2881 aagcgtcagc aacttcttac ggccatacac gtctgaaagt tctccgagaa taggagcggc
     2941 aataaactgc atgagaccga agagcgcggt gatgaggccg gcgatgaaga actgcatccc
     3001 ggtggagtag ccttggagta agaagtgcgg cgaagtcgcg tccgtgaaga taataggaat
     3061 gatgggaaac accattcccg tgccgatcat gtcgagcagc aatgtgagaa acagaatagg
     3121 aagaactttc ttttgcatac cgtattagcc gaccaaaagt ccgaacacga acatcacgaa
     3181 cgcatggaac gggcgcgcga gaagat
//
LOCUS       DAORIN010000075         3097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_7756_length_3097_cov_5.494083, whole genome shotgun sequence.
ACCESSION   DAORIN010000075 DAORIN010000000
VERSION     DAORIN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7756_length_3097_cov_5.494083"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PK109_03100"
     CDS             <1..810
                     /locus_tag="PK109_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRH24548.1"
                     /translation="SILRKGTLEGMTLPGKLADCQTKDASESELFIVEGDSAGGTAKT
                     GRDRRTQAILPLRGKILNIERARLDKMLASEQIKNLVIAMGTAIGDVFDLSKLRYHKV
                     IIATDADVDGAHIRTLLLTLLYRHFRPVIDGGYIYIAQPPLYKIKRGKEVMYAYSDEE
                     KFKLIGNDADVSEEPEEGEEETEETEEEDTKGKAKKTARVSIQRYKGLGEMNASELWE
                     TTMDPKHRVLKQVVVEDAAEADRIFDILMGTDVPSRKSFIQSNAKLANLDV"
     gene            822..1073
                     /locus_tag="PK109_03105"
     CDS             822..1073
                     /locus_tag="PK109_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24549.1"
                     /translation="MSHEDGRKDKERKVSPPTWQQADYETEIAVGETLDEAVDRARAE
                     MESRGGKSFNVFRTGERGKTIPAASERRTTGYEIKKEGK"
     gene            complement(1376..2095)
                     /locus_tag="PK109_03110"
     CDS             complement(1376..2095)
                     /locus_tag="PK109_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRH24550.1"
                     /translation="MSASPAHDSHGAPKKATINGLAVIGFFGLVIAGMLLAVYASRYV
                     PETLSRLSSAVYLSSDSANSGKATTTPTVTTKPTTPVVVTPVATTSTPELPDETGYTP
                     RTTVYNPPRVISSGPKLYGQADLALVDVEVGYLRSGRFIEDDTVPANRDMALRFTVRN
                     SGTNIASGWQVRVSVEGERDAVATGGLLYPNGYQNFELRVTNPREGRNLETEIDVDYR
                     NAVNESNERNNDDSVEVDVDD"
     gene            2167..2838
                     /locus_tag="PK109_03115"
     CDS             2167..2838
                     /locus_tag="PK109_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HRH24551.1"
                     /translation="MKRKPKVIYLPGVFERILAKKWSFLGVFVVVYFFSFNFLAGVGI
                     APGAFAFRPDPVEVPVVPTENDALILPEGQGELPVRIEIPKVGIQATVSNPNTTDVAT
                     LDRELLAGAVRYPGSGTLGENGNVLMFGHSSHLPVVHNQAFKAFNDIQNLEVGDPIFV
                     FGKEKIYVYAVEKVEAENTTTGAIPLDITGAKLTLATCDNFGTKSDRFVVTAVLVSIQ
                     DQKSQ"
     gene            complement(2914..>3097)
                     /locus_tag="PK109_03120"
     CDS             complement(2914..>3097)
                     /locus_tag="PK109_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24552.1"
                     /translation="CKLTVGIALQLNVETLHAGCRRRRERPTLNRVRLSRSAQTANDE
                     SCRKDHLLHARPLPWV"
BASE COUNT          824 a          756 c          825 g          692 t
ORIGIN      
        1 tccatactgc gtaaggggac actcgagggt atgacccttc cgggtaagct cgctgactgt
       61 cagaccaagg acgcgtctga gtccgaactc tttatcgtag agggagactc tgcaggaggt
      121 accgcaaaga cgggtcgtga tcgtcgcaca caggccatcc ttcctcttcg cggaaagatc
      181 ttgaacatcg agcgtgctcg tcttgataag atgctcgcgt cagagcagat taagaacctc
      241 gtcattgcga tgggaacggc gataggggat gtgttcgacc tctccaagct tcgctatcac
      301 aaggtcatca ttgctaccga tgccgacgtt gacggtgctc acatccgcac actcctcctt
      361 acgcttttgt accgtcactt ccgtccagtc atcgacggcg gctatatcta cattgcacag
      421 ccaccgctct acaagatcaa gcgcggcaaa gaagtcatgt acgcctactc agacgaagag
      481 aaattcaaac tcatcggaaa cgatgccgat gtttctgaag aaccagaaga aggcgaagag
      541 gagactgagg aaactgaaga ggaggatacg aagggtaaag caaagaagac tgcacgcgtt
      601 tccattcagc gctacaaagg tctcggagaa atgaacgcat cagaattgtg ggagactacg
      661 atggacccaa agcatcgtgt attgaaacag gtagtggttg aagatgctgc tgaagctgat
      721 cgcatcttcg atatcctcat gggtactgat gtaccaagtc gtaagagctt tattcagagc
      781 aacgctaagc tcgcaaactt ggacgtgtaa cgactaacac tatgtctcac gaagacggaa
      841 gaaaagacaa agaacgaaag gtttcaccac cgacctggca gcaagcagat tatgagacag
      901 agatagccgt tggagagact ctcgacgaag cagtggatcg agcacgcgcc gagatggaaa
      961 gcaggggagg aaagagtttt aatgtatttc gtacaggaga gagagggaaa accattcctg
     1021 ctgcatcgga gcgtcgtacg acagggtacg aaataaaaaa ggagggaaag taaaagaaat
     1081 aaatcgagcc ctgtgaaaac aggactcgat tcagcttcgg cttcaacgcc ttagcttgcg
     1141 gcgaacgggt tgtaaccgga aaggtacaca aacgcgccaa ctacgagcgc tatgatcagc
     1201 aagatcatcc cgatgtgaga tggtccgcca gaggttcttt tctcggccat agtagcctcc
     1261 gtttcaacgt catgcctgtg tgcacgactt aggtatcatt ctccttattt cacccactgt
     1321 caaatagcaa aaacccgcgg caaaagccgc gggtttttac ctgtcagtag ggaaattagt
     1381 cgtcgacgtc tacctcaacc gagtcgtcgt tgttgcgttc attcgattcg tttaccgcat
     1441 tacggtagtc aacgtcgatt tcagtctcaa ggttgcgtcc ttcgcgtggg ttagtgacac
     1501 ggagttcaaa attctgatac ccgtttgggt agagaaggcc accggtagca acggcgtcgc
     1561 gttcaccttc tactgaaaca cgtacctgcc agccgcttgc gatattggta cctgagttac
     1621 gcaccgtaaa gcggagcgcc atgtcgcgat ttgcgggaac ggtatcgtct tcgataaagc
     1681 gtccgctacg aaggtatcct acttcaacat ccacaagtgc aagatctgcc tgaccatata
     1741 gttttgggcc tgaggatata acgcgagggg gattatagac ggtagtgcgt ggggtgtaac
     1801 cggtctcatc cggaagttct ggggtcgagg tggtagcaac aggagtgacg actaccggag
     1861 tggttggttt agtcgttacc gttggggtag tggttgcctt cccgctgttt gccgaatcac
     1921 ttgagagata cacggctgaa gagaggcgag agagggtctc tgggacgtag cgggatgcgt
     1981 agaccgcaag cagcatgccg gcgataacca gaccaaagaa cccgatcacg gcgagaccgt
     2041 ttatcgttgc tttcttcggg gcaccatggc tgtcgtgggc tggggaagca gacatatttg
     2101 attaattact atttaccaca caataaacct attgtcaaat aagtcaagac tgcgtacact
     2161 acgtatatga agcgtaaacc aaaggtcata tacctccccg gtgtctttga acgcatcctc
     2221 gccaagaaat ggtcctttct cggggtgttc gtcgtggtct acttcttctc cttcaacttc
     2281 cttgcgggtg tagggattgc gcccggtgct ttcgcgtttc gcccggaccc agtagaagtg
     2341 ccagtggttc ctactgaaaa cgatgctctt atccttccgg aagggcaggg agaactaccg
     2401 gtgcgtattg agattccaaa agtcggtata caagccacgg tatcgaaccc gaacactacc
     2461 gatgtcgcga cccttgatcg tgaactcctc gcaggcgccg tccgctaccc aggaagcggt
     2521 accttgggtg agaacggtaa cgtccttatg ttcgggcact caagccatct ccccgtagta
     2581 cacaaccaag cgttcaaagc gttcaatgat attcaaaacc tcgaagtagg ggacccaata
     2641 ttcgtgttcg ggaaagagaa gatctacgtg tatgcagtag agaaagtgga ggcagaaaac
     2701 actacaactg gcgccattcc acttgatatt acgggagcaa aactcacgct tgcgacctgc
     2761 gacaactttg gcaccaagag tgatcgcttc gtcgtaacag cagtactcgt ttccatacaa
     2821 gaccaaaaaa gccaataact aggccgtaga gtagaccttg acaaaggtaa taaataggta
     2881 taatatactt gtattcttct taatttcagc aactcaaacc caaggaaggg gacgagcatg
     2941 aagaaggtga tccttacggc agctctcatc attagcggtc tgtgcactcc ggctcaggcg
     3001 gacacggttc agcgtgggac gttcacggcg gcggcgacac cctgcgtgca gggtctcgac
     3061 gttcagttgg agcgcaatgc caacggtcaa cttacag
//
LOCUS       DAORIN010000076         3061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_7947_length_3061_cov_4.918164, whole genome shotgun sequence.
ACCESSION   DAORIN010000076 DAORIN010000000
VERSION     DAORIN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7947_length_3061_cov_4.918164"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PK109_03125"
     CDS             complement(<1..1295)
                     /locus_tag="PK109_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRH24553.1"
                     /translation="MFSLHEPIDTTLRDELAEYDDLTAALLARRGVKSKEEAAAFLSP
                     SYDEHIGDPMKILNMPKAAARVAKALADGERITVWSDYDCDGIPGGVVLHDFLKKAGA
                     NFTNYIPHRHLEGYGVSVMGVEKLAEEGTTLMITVDCGITDVQPIKRAQDLGIDVILT
                     DHHLPQNEDEGGLPPAYAVIDPKQAGETNDFKEFCGGGLAWKLACAVLQHGFPGRENI
                     PLGWEKWLLDMAGLSTIADMVPLIGENRVIAKYGLMVMRKSPRIGLQKLLKNARVNQG
                     YITEDDVGFMIAPRVNAASRMGNPRDAFELFTTEDENRADALAKELEKINRSRRSVAA
                     AITRAVHERLEERKLQGALPDIIAMGDPDWRPGLLGLVANTVAEEYERPVFLWGREGG
                     VSLKGSCRAGVPGVSVVDIMAGAPDTFVGFGGHRASGGER"
     gene            complement(1319..1780)
                     /locus_tag="PK109_03130"
     CDS             complement(1319..1780)
                     /locus_tag="PK109_03130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRH24554.1"
                     /translation="MKKDGCIFCMIVAHEAPSHPIWENDSYLAFLSIFPNTEGFAVVI
                     PKEHHDSYFADAPGPILMGLMQAAAAVAKRIDAAYPDVGRTGLIFEGFGVDHLHAKLV
                     PMHGTSSDEWKQHHSKEKRFTEFYEGYISSHDSERADDAVLAEIAKKIRGE"
     gene            complement(1785..2918)
                     /locus_tag="PK109_03135"
     CDS             complement(1785..2918)
                     /locus_tag="PK109_03135"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRH24555.1"
                     /translation="MRSVRDIKVLENIPVLLRTSLNAPIENGVVTNAFRLKSALPTIQ
                     YLRERHARVVLISHISGKGTETLEPMYEAMKQWLPDIAFCPVATGAQARAAVRDLLPG
                     GVLMLENLRRNVGEEKNDGEFAKELAELADVFVQDSFDVCHREHASVVGVPGLLPSYA
                     GLTLEQEVKELTKALKPKHPAVAIIGGAKFSTKEPVLKKLLKTYDHVFVGGALANDFL
                     KAKGYSVGTSLVSDEGQDHIKKLLKDKRLIVPVDVIAAPLKSTRDAGRTAMANEVKPD
                     EAVLDAGPHTAAALVDLINKSKTVLWNGPLGLFENGFSDATRMVARAVVGAHTHSIIG
                     GGDTIAAVEEMKPASDFSFISTGGGAMLDFLAHGTLPGIKALK"
BASE COUNT          661 a          914 c          766 g          720 t
ORIGIN      
        1 cgctccccgc ctgacgctcg gtgcccgccg aaccccacaa acgtatcagg agcgcccgcc
       61 ataatatcaa ctaccgatac tcccggtact cctgcgcggc acgaaccctt aagtgacacg
      121 ccgccttctc ttccccagag aaacacggga cgttcatatt cttctgcaac cgtgttcgcc
      181 actaacccca agagtccggg acgccaatca gggtccccca ttgcgatgat gtcggggagc
      241 gctccttgaa gctttctctc ttcaagacgt tcatgcacgg cgcgcgtaat agcggcagcc
      301 accgagcggc ggctccggtt tatcttttcc aattcttttg cgagtgcgtc ggcacgattc
      361 tcgtcctctg tcgtaaagag ctcgaaggcg tcacgcgggt tccccatacg agacgctgcg
      421 ttcacgcgcg gcgcaatcat aaagcccaca tcgtcctcgg tgatatatcc ctgattcacg
      481 cgcgcattct tcagaagctt ctgaaggccg atgcgaggcg atttacgcat caccatgagt
      541 ccatatttag cgatgacgcg attctctcca ataaggggca ccatgtcagc gatggtagag
      601 agtcctgcca tatcaaggag ccacttctcc cacccaagcg gaatgttctc gcgtcccggg
      661 aacccgtgtt ggagcaccgc gcacgcaagc ttccaggcaa gcccgccgcc gcagaactct
      721 ttaaaatcat tcgtctctcc tgcctgctta ggatcaatga ccgcatacgc cggcggtagc
      781 cctccttcgt cttcgttttg cggcaggtga tgatccgtaa gaatgacatc aatgccgagg
      841 tcttgtgcgc gtttaatggg ctgtacgtcc gtaataccgc aatccactgt gatcatgagg
      901 gtagttccct cttccgcgag tttctcaacg cccataactg atacgccata cccttcaaga
      961 tggcgatgcg gaatatagtt cgtgaaattg gcccctgctt ttttgagaaa gtcatggagt
     1021 accactccac ccgggatgcc atcgcagtca taatcgctcc agacggtaat gcgctcaccg
     1081 tccgcaagtg ccttagccac gcgagcagct gctttcggca tattcagaat cttcatcggg
     1141 tccccgatgt gctcgtcata cgagggagag agaaacgctg ccgcttcctc cttgcttttc
     1201 acccctcgac gcgccaagag cgctgccgtt aaatcgtcat attcagcaag ctcatcgcgc
     1261 agagtggtat caatgggctc atggagggaa aacatcgttc cccgattgta ccaaatcgct
     1321 attccccgcg tattttcttg gcaatttccg ctagtacggc gtcgtcagca cgttccgagt
     1381 catgagagga aatatagcct tcatagaact ccgtaaagcg cttctcttta gagtgatgct
     1441 gcttccactc gtcactactc gtgccatgca tcggaacgag cttcgcatgg aggtgatcga
     1501 cgccgaaccc ttcgaatatg aggcccgtgc gtccgacgtc agggtatgcc gcgtcaatgc
     1561 gtttagcgac cgccgctgcc gcctgcataa gtcccatgag gataggtccc ggtgcgtcag
     1621 caaagtaact atcgtgatgc tcttttggaa taaccacggc aaatccttcc gtgttcggga
     1681 atatagaaag gaaggcaagg tacgaatcgt tttcccaaat agggtgcgac ggcgcctcgt
     1741 gcgcgactat catgcagaaa atacagccgt ctttcttcat atcgttattt taacgcttta
     1801 attccgggaa gcgtaccgtg cgctaaaaag tcgagcatgg cacctccccc cgttgagatg
     1861 aaggagaagt cagacgcagg cttcatctcc tctacggctg caatagtgtc tcccccgccg
     1921 atgatggagt gcgtatgtgc acctactacc gcacgcgcca ccatgcgcgt tgcgtcagag
     1981 aacccgttct caaataggcc gagaggacca ttccacagca cggtcttcga cttattaata
     2041 agatcgacga gtgccgcagc cgtgtgcgga cctgcatcaa gtaccgcttc gtctggtttc
     2101 acttcgtttg ccatggcggt gcgccccgcg tcccgcgtgc tctttaaggg agcagcgata
     2161 acgtccaccg gaacgatgag acgcttatcc ttcaggagtt tctttatgtg gtcttggcct
     2221 tcgtcggaaa cgagcgaggt gcccaccgaa tacccttttg cttttaagaa atcatttgca
     2281 agcgcccctc ccacaaacac atggtcatag gttttcaaaa gctttttcag taccggctct
     2341 ttcgtcgaaa actttgcgcc acctatgatg gcaaccgcag gatgctttgg tttgagtgct
     2401 ttcgtgagct ctttcacttc ttgttcgagc gtgaggcctg cataggaggg gaggagtccg
     2461 ggaacgccaa ccaccgacgc atgttcgcgg tggcacacat caaaggagtc ttgcacgaat
     2521 acgtccgcaa gctcggcaag ctcttttgca aactcgccat cgttcttctc ttccccgacg
     2581 ttgcggcgta ggttctcaag catgagaacg ccacccggaa gaaggtcgcg tacggctgcg
     2641 cgtgcctgtg ctccggttgc tacgggacag aaggctatgt caggaagcca ctgcttcatg
     2701 gcctcataca taggctcgag cgtctccgtt cccttcccgc ttatatgact gatgagcacc
     2761 acacgcgcat gacgctctcg taaatactga atggtcggca gtgcgctttt aagccggaac
     2821 gcgttggtga ccacgccgtt ttcaatgggt gcattcaatg aggtacgtaa cagtactggg
     2881 atgttctcga gcaccttgat gtcacgaacg ctacgcatga attagacgag cgcttttact
     2941 aaagcgctaa aggtagcggt ctccgcgctc gcgtgcccta ccaaaaaccc atccacccca
     3001 cttccgcccg ctatcgcccg cacatttccc gactccacgg cgcctccata gaggaccttt
     3061 g
//
LOCUS       DAORIN010000077         2977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_8401_length_2977_cov_5.041410, whole genome shotgun sequence.
ACCESSION   DAORIN010000077 DAORIN010000000
VERSION     DAORIN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8401_length_2977_cov_5.041410"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /gene="rplD"
                     /locus_tag="PK109_03140"
     CDS             complement(<1..325)
                     /gene="rplD"
                     /locus_tag="PK109_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRH24556.1"
                     /translation="MATTKKAPAKKTTAKKAAPRAPKKAPVIEVGKGEAVIYGMNGAA
                     AGTVTLPAELFGAKWNADLVHQVVTGMQANARPTVAHTKFRGEVSGGGKKPWKQKGTG
                     RARHGS"
     gene            complement(337..945)
                     /gene="rplC"
                     /locus_tag="PK109_03145"
     CDS             complement(337..945)
                     /gene="rplC"
                     /locus_tag="PK109_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRH24557.1"
                     /translation="MKFLLARKERMTQIFQEDGKALAGTLLKMSPSTVTQVKTADGAD
                     GYSAVQIGFDAQKPSRVAKPQQGKAFKVIKEYRVENPADYTVGAEIGTDTFAVGDEVR
                     VVGTSKGRGFAGVVKRYNFRGGPRSHGQKHSERERGSSGGGPGRAGGRVAPGMKMAGR
                     MGNARVTVKNLRVIHVDQAAGEIIISGAVPGARGSLVEIIAA"
     gene            complement(1081..2055)
                     /locus_tag="PK109_03150"
     CDS             complement(1081..2055)
                     /locus_tag="PK109_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24558.1"
                     /translation="MEHPNEYIDARLARLNVVNDLPTFANDEERKTFIVKTILSKKYR
                     KYAVDGDFLPHLEGALEYSFKNGKPLQFVYPFGGYKLWRLDESPEADWAELFTMMHNA
                     AWLKSIAHVYEPGVWFDFSPDDVIVERMNHIPKADTDAYRATFNEVIAFITPYLPENL
                     KFTLTPVGSRYTPEEFDADLTARTDELLAQNHGKLPQASDAEKAMMDLNAKRTPEELA
                     DPEWYGKNKLMHDAYMATSKRRPYNRAPEKILTFTKALPKGVAIGSTKTSIAKFWAGV
                     GALKKRDDSYIETILSPEQIKAASATWEPISIGLKGKNFSKIRIIESS"
     gene            complement(2055..2130)
                     /locus_tag="PK109_03155"
     tRNA            complement(2055..2130)
                     /locus_tag="PK109_03155"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2094..2096),aa:Cys,seq:gca)
     gene            complement(2230..2721)
                     /locus_tag="PK109_03160"
     CDS             complement(2230..2721)
                     /locus_tag="PK109_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24559.1"
                     /translation="MRITPVMVYDAAIRIDAALKRHSIIGPILREKKRFGCVVVFTDD
                     AGDIVIQAVIGEKPDDCLGKHAFAREKIGRLMLMKHRHGHVLSRQSQDEHADPPQYAG
                     AVMINEWGSSISGYPSLVDELFAVALLCQLGLITVEEALTVFRSFPNRYLDENRTEDL
                     ALL"
     gene            complement(2828..>2977)
                     /locus_tag="PK109_03165"
     CDS             complement(2828..>2977)
                     /locus_tag="PK109_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRH24560.1"
                     /translation="TFVHKDAREQFEMRIHKRLIDILSPNPKVIEALTNLTLPSGVTI
                     DVKMV"
BASE COUNT          674 a          774 c          755 g          774 t
ORIGIN      
        1 tagaaccatg gcgagcgcgg ccagttccct tctgcttcca tggctttttg ccaccgccag
       61 atacttcgcc gcggaacttg gtgtgagcga ctgttgggcg agcgtttgcc tgcataccgg
      121 tgaccacctg gtgcacaagg tcggcgttcc actttgcacc aaagagttct gcagggagcg
      181 taaccgtacc agctgcagca ccgttcatgc cgtagataac ggcttcaccc tttccaactt
      241 caataaccgg agctttcttg ggagcgcgcg gtgctgcttt tttagcagtc gtcttctttg
      301 ctggagcttt cttggtagta gccataagta tataaattat gcggcgatga tttcgaccaa
      361 agagccgcgt gcgccaggaa cagcacctga gatgatgatc tcaccggctg cctgatctac
      421 atgaataacg cggagattct ttacggtaac gcgagcgtta cccatgcgtc cggccatctt
      481 cataccagga gcaacacgac ctccggcacg accaggacca cctcccgaag aaccgcgctc
      541 gcgctcagaa tgcttctgac cgtggcttcg tggcccgccg cggaagttgt agcgtttgac
      601 gacgccggcg aagccacgac cctttgaggt accgacgaca cgaacctcat cccctaccgc
      661 aaaagtatca gtaccaattt cagcacctac cgtgtagtca gctggattct cgacgcgata
      721 ttctttaatg accttgaagg cctttccttg ctgtggcttc gcaacacgtg atggcttctg
      781 cgcatcaaac ccgatctgaa cggcagagta gccgtccgca ccgtccgcag tcttcacctg
      841 agtgacggta gaaggactca tcttaaggag agtgccggca agggctttgc cgtcttcctg
      901 gaagatttgg gtcatgcgct ctttgcgagc aagtaggaat ttcatgaggt aaagaaaatc
      961 gcgctcaata gcgcgttcgg tccccgttag ggggacttcc cggccattgg tccgtcccga
     1021 tggacgtacg tagcgatagg aggactatac cggagtgggt ttgaatatgc aagaacgtcc
     1081 ttatgagctc tcaattatcc ggattttact gaaattcttt ccttttaaac caatagatat
     1141 gggctcccag gtcgccgatg cagccttgat ctgttcagga gacagaatcg tttcaatgta
     1201 actatcgtcc cttttcttga gagcaccaac gccggcccag aactttgcta tcgaggtctt
     1261 ggtgctccct atcgcaacac ctttaggcag tgctttggta aaggtcagta ttttctctgg
     1321 cgctcggttg tacggacgtc gttttgacgt tgccatataa gcgtcatgca taagcttatt
     1381 ctttccatac cattctggat cagctaactc ttcaggagta cgttttgcgt ttaaatccat
     1441 cattgctttt tctgcgtctg acgcctgtgg aagcttcccg tgattttgag caagtagttc
     1501 gtcggtacga gctgtaagat ctgcatcaaa ttcttcgggc gtataacgtg aaccgacagg
     1561 agtgagagtg aatttcaggt tctctggcag gtacggtgtg ataaaagcga tgacttcgtt
     1621 gaaggttgct cgataggcat cggtatctgc cttaggaata tgattcatcc gctcgacgat
     1681 gacgtcgtct ggcgaaaagt cgaaccatac tcctggttcg tatacgtgtg ctatcgattt
     1741 gagccatgct gcgttgtgca tcatcgtaaa aagctcagcc cagtctgcct caggagactc
     1801 atcgagacgc cacagtttat agccaccgaa cggatatacg aactgcaacg gttttccatt
     1861 cttaaagcta tattcgagag caccttcaag atgcgggaga aaatcaccat ccactgcgta
     1921 tttacggtat ttttttgaaa gaatcgtttt tacgatgaac gtcttacgtt cctcatcgtt
     1981 tgcaaaggtt ggaagatcgt ttacgacatt tagtcgcgca agacgtgcgt ctatatactc
     2041 gtttggatgt tccatggaga tgcaggtagg agttgaacct acgtctcttg ccttgcgggc
     2101 aagcgcctga accgtctcgg cgaccgcatc aaggcgagta tagcaaaaac atcatctctt
     2161 gaaataagaa aaggccgcca ggagaaccgg cggccttcgc tgcgtcatcg ctttcgcgac
     2221 ttagagttgc tagaggagcg ctaggtcctc tgttcgattc tcatcgaggt atctgttggg
     2281 gaaactacgg aataccgtaa gcgcttcctc aactgttatg aggcccaact gacagagaag
     2341 ggcgacagcg aataactcat cgaccaggga tgggtagcca gaaatgctac taccccattc
     2401 gttgatcatc actgcccctg catactgcgg aggatctgcg tgctcgtctt ggctttgacg
     2461 actcaacacg tgtccgtgcc gatgcttcat cagcatcagt cgcccgatct tctcccgagc
     2521 aaatgcatgc tttccgagac aatcgtcggg tttttcaccg ataaccgctt ggataactat
     2581 gtcacctgca tcgtcggtaa agaccaccac gcatccgaac cgtttctttt ctcgaagaat
     2641 cggaccgatg attgagtggc gtttgagtgc cgcgtcgatc cttattgccg cgtcgtacac
     2701 catcactgga gtgatacgca tgagtgaggc cctccttaag aacctctagg tgagttatga
     2761 catacaaaaa tcagtaacgc aaaaccgccc catctctggg gcggttttgc tgtcttccct
     2821 ttcggggtta gaccatcttc acgtctatcg tcactccgga cggaagggtg aggttcgtga
     2881 gagcctcaat caccttcggg tttggagaga gaatatcgat gagacgcttg tggatgcgca
     2941 tctcgaactg ctcacgtgcg tctttgtgga cgaacgt
//
LOCUS       DAORIN010000078         2855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_9053_length_2855_cov_4.226429, whole genome shotgun sequence.
ACCESSION   DAORIN010000078 DAORIN010000000
VERSION     DAORIN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9053_length_2855_cov_4.226429"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="PK109_03170"
     CDS             <1..1425
                     /locus_tag="PK109_03170"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRH24561.1"
                     /translation="QNTAGETIEVPITSSMTEGLNPVEYFMSTHGARKGLTDTALGTA
                     RAGYLTRRLFDVAQDSIVTETDCGAKTGIAIRRISANGIKIDFVEAIRGRTLAEAAGS
                     LKKGHYLTADDARALNADASIESVIVRSPMACVTRYGICAACYGMDLTTQQVVDLGEA
                     VGTVAAQAIGEPGTQLTMRTFHAGGTASVGGDITSGLPRVEEVFEKRSPKNPAVVSKT
                     DGMVTEIRELGREKIIVVAPEAGQTAKKVGRGKKPVDGAAIEYMVAYPRMALVKAGDA
                     VTRGQLITDGSADLDELFEYAGRAAVQEYIIAETSKIYELQGASVSRKHLEVVVKQMF
                     SRAKITDTGDSDYSVGDVVEDWEYAAAIKEADAEGKIAPKAREMVLGIKESALSRRSF
                     LSAASFEQTTKILIGAALKGSVDTLRGLKENVILGRLIPAGTGFPGSKKRAAIDALQT
                     ARNSRLAAEAEQEEMASRALTETR"
     gene            1458..>2855
                     /gene="dnaG"
                     /locus_tag="PK109_03175"
     CDS             1458..>2855
                     /gene="dnaG"
                     /locus_tag="PK109_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRH24562.1"
                     /translation="MADTVERVKEKLSIQDVVAPYVKLTKAGKYWRGLSPFNKEKTPS
                     FYVNPERGSYYCFSSSQGGDIFTFIEKMEGVDFKGALKILAEKAGVELVYEGKKDTGK
                     QDRVREAMARAEAFYARNLDEKGEAYAYALSRGLTPETIRAWNLGLAPDGWRTLLEEL
                     SAAGFSTKEMLDAGLIKEADGKPGTYYDRFRNRLMFPIKDGAGRTVAFTGRALTPEDP
                     AKYLNSPETELYKKSQILFGMDKAKDAIRTRGFALLVEGQFDLILLHQIGFTNTIALS
                     GTALSETHLSLVKRYADNLMLALDADRAGLAASAKNAYAALKAGMRVKAVRMPLGKDP
                     ADLASETPQDFVRRVSESEPIIEFFLSVLSASEKDAHRLVIAVEHVVLPLIAIMRSPM
                     EREHFIGIVARAMGSSAEAVRASVPKTAAAPEDVVAAPVAAPAVSETPEAARGLLILA
                     AIDAYAGTPLAKRLSD"
BASE COUNT          638 a          807 c          813 g          597 t
ORIGIN      
        1 cagaacacgg ccggcgagac catcgaagtg cctattactt cttcgatgac cgaaggcttg
       61 aacccggtgg agtacttcat gagtacccac ggtgcacgta agggtttgac tgacaccgca
      121 cttggtactg cgcgcgccgg ctacctcacg cgccgcctct tcgacgttgc gcaggactcc
      181 attgttacgg aaacggactg tggtgctaag acgggtatcg ctatccgccg catctcagca
      241 aacggcatta agattgactt cgttgaagcc attcgtggcc gcacgctcgc tgaagctgca
      301 ggttctctta agaagggtca ctacctcacg gcagatgatg cacgcgcatt gaacgctgac
      361 gcatcgattg agtccgtcat tgttcgctcg cctatggcct gcgttactcg ctatggtatc
      421 tgtgccgctt gttacggtat ggacctcacg acccagcagg ttgttgatct cggagaagcc
      481 gttggtaccg ttgccgcaca ggcaatcggt gaaccgggta ctcagctcac catgcgtacc
      541 ttccatgccg gaggtacggc atccgttggt ggcgacatca cctcaggtct cccacgcgtt
      601 gaagaagtat tcgagaagcg ctcaccgaag aacccagctg tcgtctctaa aaccgacggt
      661 atggtcacgg agatccgtga acttggacgc gagaagatca tcgttgtggc tcctgaagcc
      721 ggacagaccg caaagaaggt aggtcgtgga aagaagcctg tggatggtgc tgctatcgag
      781 tacatggtgg catacccacg tatggcgctc gtgaaagcag gtgacgccgt tacccgcggt
      841 cagctcatca cggacggttc cgctgatctt gatgaactct tcgagtacgc aggacgcgct
      901 gccgtacagg agtacatcat tgcggagacc tcgaagatct acgaactcca gggcgcttcc
      961 gtatcccgca agcaccttga agtggtcgtg aagcagatgt tcagccgcgc aaagattact
     1021 gacaccggtg actctgacta ctcagttggt gacgtggttg aagattggga atacgctgca
     1081 gcaattaagg aggcagacgc agaaggaaag attgcaccga aggcacgcga aatggtgctt
     1141 ggtatcaagg agtcagcgct cagccgccgc tccttcctct ccgctgcctc cttcgagcag
     1201 acgacgaaga ttctcatcgg tgccgcactt aagggctccg tggacacact tcgcggtctt
     1261 aaggagaacg tcatccttgg tcgcctcatc cctgcaggta ctgggttccc aggctccaag
     1321 aagcgcgccg ctatcgacgc acttcagacg gcacgtaact cacgcctagc tgctgaagca
     1381 gaacaggaag agatggcatc tcgtgcgctt accgaaactc gttaacggct aaccgcccgc
     1441 acgggcctac acaaagcatg gctgacaccg tagagcgcgt taaagaaaaa ctcagcatcc
     1501 aggatgttgt ggcgccctac gtaaaactca ccaaggcagg gaagtactgg cgtggtcttt
     1561 ctcccttcaa taaggagaag acgccttcct tctatgtaaa cccggaacga ggttcgtact
     1621 actgcttctc aagttcccaa ggcggcgaca tcttcacctt cattgagaag atggaaggcg
     1681 tcgacttcaa aggcgcgctc aaaatcctcg ctgagaaagc gggggttgag ctcgtgtacg
     1741 aagggaagaa agatacggga aaacaagacc gcgtgcgcga agcgatggct cgtgctgaag
     1801 cgttctatgc ccgaaacctt gatgagaaag gggaagcgta tgcgtacgca ctctcgcgtg
     1861 gtctcacccc agaaactatt agagcgtgga atcttggcct tgcgccggat ggctggcgta
     1921 cgcttcttga agagttgtct gcggctgggt tctctaccaa agagatgctc gacgcaggtc
     1981 tcatcaagga agcagacgga aagcccggca cgtactatga ccgcttccgt aaccgactca
     2041 tgttcccgat taaggacgga gccgggagga cggtggcgtt cacgggccgt gcgctcaccc
     2101 ctgaagatcc tgcgaaatac ctgaactctc cggagacgga gctctataag aagtcgcaga
     2161 tactcttcgg gatggataaa gccaaagacg cgatacgtac gcgtgggttc gcgcttctcg
     2221 tggaagggca gtttgacctc atccttctgc atcagattgg gttcacgaac accatcgcgc
     2281 tttcgggtac cgcgctctct gagacgcact tatcgctcgt gaagcgctac gccgacaatc
     2341 tcatgctcgc gcttgatgcg gaccgtgcgg gactcgcagc gtccgctaag aatgcgtacg
     2401 ctgcccttaa ggcaggcatg cgcgtgaaag cggtccgcat gccgctcggg aaagatccgg
     2461 cagatcttgc aagcgaaacc ccgcaggact ttgtccgtcg tgtgtctgag tcagaaccca
     2521 tcatcgaatt cttcctctcc gtattgtccg caagtgagaa ggatgcgcat cgtctcgtca
     2581 tcgcagttga acatgtggtg ctcccgctca tcgccattat gcgtagcccc atggagcgcg
     2641 agcatttcat cggcatcgtt gcgcgcgcga tggggtcgtc agctgaagcg gtgcgcgcgt
     2701 ccgtaccgaa gacggcagct gctccagaag atgtggttgc cgctccggta gcggctccag
     2761 cggtttctga aacaccagaa gctgcccgtg gcctcctcat ccttgcggct atcgacgctt
     2821 atgcgggaac cccccttgca aaacgcctct cggac
//
LOCUS       DAORIN010000079         2840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_9146_length_2840_cov_4.504129, whole genome shotgun sequence.
ACCESSION   DAORIN010000079 DAORIN010000000
VERSION     DAORIN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9146_length_2840_cov_4.504129"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..512)
                     /gene="tsaE"
                     /locus_tag="PK109_03180"
     CDS             complement(75..512)
                     /gene="tsaE"
                     /locus_tag="PK109_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRH24563.1"
                     /translation="MEISVATPEGLAEAAASFLTELVPSTTGATLITLSGDLGAGKTA
                     FTKALANELGVTDSVTSPTFVLEKVYLLPGYGTWKRLIHIDAYRLKSGEELAALGFDE
                     AMHDPQTLIVLEWPEQVSDALPKPTAALRITASPDGTRTLSYD"
     gene            1184..>2840
                     /locus_tag="PK109_03185"
     CDS             1184..>2840
                     /locus_tag="PK109_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRH24564.1"
                     /translation="MPSHFDTDAEDAKLAALREKEEEDLARILSDKYGLTYTDLSVVP
                     VNVDALRLVQEDKARAAEMVVFDKAGKALSIALHSPNNPALAGIFEGLESRGFTLQKF
                     LVSARSLKRALDRYADLSYAHVTTEGVFDISSEDINKLEKEFDSLASLKSHLDEVLST
                     KKTAQVSRIFEDIVAAALSLGASDIHLEPEEHAVRLRLRLDGMLSDAYMFDPKVYHSL
                     NSRVKLLSGLKLNVDNRAQDGRFTIAVQGTEIEVRTSVIPGNYGESLVLRILDPNTLT
                     RSFEDMGVNPKLLARLREEIKRPNGMLLTTGPTGSGKTTTLYTFLLALHTPETKIVTI
                     EDPIEYHLEGIVQTQTNGKDYTFASGLRSVLRQDPDVILVGEIRDGEVAEVALQAALT
                     GHFVFSTLHTNNAAGTFPRLVDLGADPKVFASAVSVAMGQRLMRTLNPETKKARPATE
                     EEKKLMQAIMATVTDTSLLPASYDTVYDAVPDENGGTGYKGRLGVHEAIFMDEELGEF
                     LRDNPSEGDIAKHVLRQGYLTMAQDGVLKVLAGQTTLEELARTV"
BASE COUNT          666 a          792 c          736 g          646 t
ORIGIN      
        1 gaagtgcgtg atacgcgcga tgaatgattg catgtgcgtc taacagaatg agccgcttct
       61 tggtgagaga ttttctaatc ataagaaagg gtgcgcgtgc cgtcgggtga agcggtaatg
      121 cgaagggctg cggtaggttt cggcagtgcg tcgctcactt gttcgggcca ctcaagcacg
      181 atgagtgttt gtgggtcatg catcgcttca tcgaacccaa gcgcagcaag ttcttcccca
      241 cttttcaagc gatacgcatc aatatggatg agacgtttcc acgtaccgta accaggaagt
      301 agatatacct tctcaagaac gaaggtgggg ctcgtcaccg agtcggtaac cccgagttcg
      361 tttgcaagtg ctttagtgaa cgcagtcttc cctgccccaa ggtcacccga aagggttatg
      421 agcgttgctc ccgtagttga aggcacgagt tctgtaagaa aagaggctgc tgcttcagca
      481 aggccttcag gggtcgcaac agatatctcc atctccctat tgtaccaatg tatacaaata
      541 gggcccgaca tgatcgctca tgccaggccc caatgtcgca gggctccgta ttacgggagc
      601 cattcagtga cgataccgta gggtacggtg tcgtggactc gcgccgcctc agctgcacac
      661 catgcccgct gtctgttggt ttcgctctct ttaatttcga gaggagctgc cacagacaaa
      721 cgaacggtgc acacaccagg aactacttgc ccacgggcga ggacttcggt acggccccca
      781 ccaagaaaca ctcgttggcc gacttgggga cgcacagtgt gtctgtgtcc cccaaagaag
      841 aagtcgagcg cggcgtacgt gacacccgcg cctatggagc cgtaattgta gttttggcct
      901 ccaccgcggt aagggccacc cccaccgcct cgttcctcat gcactcttat gcgcactcct
      961 tcgtgcacgc gcatgtaccc atggcccgac tgcgccgaag cgcttggggc cgtcactaat
     1021 actgcaattg ccgtcaaagc ggcaacgatg aacgtccgga ccattcacga cctcgtttct
     1081 aatttaaagt cctccggaaa gttacacaat aaccataaaa agatgctttg tcaatgacga
     1141 gaaagcgtca tttaggctcg tgctgatgct atgatttccc gtaatgcctt cccattttga
     1201 tactgatgct gaggacgcta agctcgccgc tctgcgtgaa aaagaagagg aagacctcgc
     1261 acgtatcctt tctgacaaat acggccttac atatacggac ctctctgtgg tgccggtcaa
     1321 cgtcgatgca ctccgtttag tacaagaaga caaggcgcgc gccgctgaga tggtggtctt
     1381 tgataaggcg ggcaaggcgc tctctatcgc gcttcatagt ccaaacaacc ccgctcttgc
     1441 cggtatattc gagggacttg agagtcgtgg ctttacgctt cagaagtttc tggtgtctgc
     1501 ccgaagcttg aagcgtgctc tagaccgcta cgccgatctc tcctacgctc atgtcactac
     1561 tgaaggagtg ttcgatatct caagtgaaga cattaataag ctcgaaaaag agttcgactc
     1621 cctcgcaagt cttaagtcgc atcttgatga ggtgctctca acgaagaaaa cagcacaggt
     1681 ctcccgcatc tttgaagata ttgttgctgc tgccctttca ctcggcgcgt ctgacattca
     1741 tctcgagcca gaggagcacg ccgtgcgcct tcgtcttcgc cttgatggca tgctctctga
     1801 cgcctatatg ttcgacccta aggtgtatca ctcactgaat tcccgcgtga agcttctctc
     1861 ggggttaaaa ctcaacgttg ataaccgcgc acaagacgga cgcttcacca ttgcagtcca
     1921 aggcaccgaa attgaagtgc gcacgtccgt cattccaggt aactatggtg agtccctggt
     1981 gcttcgtatt cttgacccga acactctcac ccgctcgttt gaagatatgg gggtgaaccc
     2041 taagctcctt gcgcgtctgc gtgaagagat taaacgcccc aacggcatgc tcctcaccac
     2101 cggcccaaca ggctcgggta agacaacgac cttgtacacg ttccttctgg cgctccatac
     2161 gccggaaaca aagatcgtca ccattgaaga cccaattgag taccaccttg aaggcattgt
     2221 gcagacccaa acgaacggca aggactacac gttcgcatca ggacttcgct ccgtgctccg
     2281 tcaggacccc gacgtcattc tcgtgggaga aattcgtgat ggtgaggtag cggaagttgc
     2341 gctccaggcg gcactcaccg gtcacttcgt gttctcaacg ctccacacca ataacgccgc
     2401 gggcaccttc ccccgcttgg ttgaccttgg tgctgatcca aaagtatttg cgtcagcagt
     2461 gtcagttgcg atgggtcaac gcctcatgcg cacgttgaat ccagaaacga agaaggcgcg
     2521 ccctgctacc gaagaagaaa agaagttaat gcaggccatc atggctacgg ttaccgatac
     2581 ttcccttctc cctgcgtcgt acgacacggt gtatgacgcg gtgcccgacg agaatggtgg
     2641 taccggatac aagggccgac tcggcgtaca cgaagccata ttcatggacg aagaacttgg
     2701 agagtttttg cgtgataacc catcagaggg tgacatcgca aagcacgtgc tgcgtcaggg
     2761 atacctcacc atggcacagg acggcgtgct taaagtactc gcaggacaaa cgactcttga
     2821 agaattagcg cgtacggttg
//
LOCUS       DAORIN010000080         2767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_9607_length_2767_cov_3.535398, whole genome shotgun sequence.
ACCESSION   DAORIN010000080 DAORIN010000000
VERSION     DAORIN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9607_length_2767_cov_3.535398"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1657)
                     /locus_tag="PK109_03190"
     CDS             complement(<1..1657)
                     /locus_tag="PK109_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24565.1"
                     /translation="MFVVRRHPENPLLSPQREHPWEALATFNPSVVKTETGVKMYYRA
                     FAHPAALIAPFAPQSTIGVADSEDGVHFHSQRQVITPKEAWEAFGCEDPRATVIDGVT
                     YLSYTALGGYPFNADNIRVAIAVSKDGEHFDERHLATPFNAKAFALFPEKVGDKYAAL
                     LTVHTDQPPSKICLALADSIEDYWNPDYWMKWYADWESHALEFVRSDKDHVEAGAVPI
                     KTERGWLFFYSYIEHYFGDGRRVFGIEAALLDAADPMKVSGRTYPFLVPEEIYERYGV
                     EPEIVFPTSALDTGDGMIDLYYGAADTTVAKATIRTKDLLDSIDTSVSPVLMRALENP
                     ILLPRKENAFEAKLVFNPAAIDLEGSIHILYRAMSEDNTSYVGYARSEDGLHIDERLP
                     VPIYGPRADFEMKLSHPTGNSGCEDPRAVVIDGRIYMTYTAYDGIHAPRGAVSSISTE
                     DFLAKKFDAWTEPFLVTPDDVDDKDLALLPEKTADGHFMLYHRISGRICADILPDLTS
                     GTRISRCIEIMGPREGMWDAAKVGMAGPLLKVDGGWLMLYHGVS"
     gene            complement(1665..>2767)
                     /locus_tag="PK109_03195"
     CDS             complement(1665..>2767)
                     /locus_tag="PK109_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24566.1"
                     /translation="TGLFLYALWKLIRTHAIRERIVEFARSLGLLREHGNPILVPRGQ
                     SWEAGGVMNPAAIEAGGRVHLFYRAVGNDGVSRIGYASSKNGASFDERLPYPVFALAG
                     GDDATRRFAEHSGLSKSGGSFAGVEDPRAVVIGDRMYMTFSAFGGWDALRIGVSSIAI
                     SDLLAKRWKWTAPTYLSPVGQVHKNWVLFPEKINGKFAVLHSLHSGSRDRVLVDYLDT
                     LDENASIESPYNPVKDESVWDSTLRGAGPPPIKTKDGWLLFYHATDAREPSRYKLGAL
                     LLDLTDPTKVIARSPFPILSPDATYENEGFKSGVVYACGATVHGDTLTVYYGGADSVV
                     CAAKISLSEFLKKLKDKKMARLTPVPLRFSAA"
BASE COUNT          654 a          722 c          774 g          617 t
ORIGIN      
        1 gtgagacgcc gtgatagagc atgagccagc caccgtcgac tttaaggagc ggacccgcca
       61 tacctacttt ggctgcgtcc cacatacctt cacgtggtcc catgatttca atgcagcggg
      121 atatgcgcgt gccgctcgtg agatcaggaa ggatgtccgc acagatgcgt ccactgatgc
      181 ggtggtagag catgaagtgg ccgtcggccg tcttctcggg aaggagcgcc aaatctttat
      241 catccacatc gtcgggggtc acgagaaatg gctcggtcca tgcatcgaac ttctttgcga
      301 gaaagtcttc ggttgagata gaggagacgg caccgcgtgg cgcgtgaatg ccgtcatatg
      361 cggtataggt catgtagatg cggccatcga tgacaacagc gcgcgggtct tcgcacccgg
      421 agttacctgt cgggtgagag agcttcatct caaagtcagc acgaggtccg tagataggta
      481 cgggaagtcg ttcatcaata tggagtccgt cttctgagcg tgcgtatcct acatacgacg
      541 tattatcctc actcatcgcg cgatacagaa tatgaataga accctcaaga tctatagctg
      601 cagggttaaa cacgagcttt gcttcaaaag cgttttcttt ccgcggcagc agtatcggat
      661 tctcaagagc gcgcattagt accggcgata cactcgtatc aatggagtcg agtaggtcct
      721 tggtgcgtat cgtcgcttta gcaacagtag tgtcagcggc accataatat agatctatca
      781 tgccgtctcc cgtgtctagc gctgaggtag ggaagacaat ttcaggttcg accccatagc
      841 gctcatatat ttcttccggc acgaggaacg gataggtgcg tcccgaaacc ttcatagggt
      901 cagccgcatc aagaagcgcc gcttcaatac cgaagacacg gcgtccatct ccgaagtaat
      961 gctcaatgta ggagtagaag aagagccagc cgcgctccgt tttaataggg accgctcctg
     1021 cctccacgtg gtctttgtcg cttcgcacga actcaagtgc gtgtgattcc cagtcggcat
     1081 accatttcat ccagtagtcg ggattccagt agtcttcaat agagtcggca agtgcaagac
     1141 aaatctttga aggtggctga tcagtgtgca cggtaaggag cgctgcgtac ttatcaccga
     1201 ccttctcagg gaagagtgca aatgcttttg cattaaaggg agtcgcgaga tgacgttcgt
     1261 cgaaatgctc accgtccttg gagacagcga tagcgacacg aatgttatcc gcattgaacg
     1321 gatagcctcc gagtgccgtg tacgaaagat acgtgacgcc atcaatgacc gttgcgcgcg
     1381 ggtcttcaca cccaaacgct tcccatgctt cttttggcgt gatgacctga cgctgtgagt
     1441 ggaagtgaac accgtcttct gagtcagcaa caccaatggt ggattgaggc gcaaagggcg
     1501 cgataagggc tgcggggtgc gcaaaggcgc gatagtacat cttcacccct gtttccgttt
     1561 taacgacaga gggattaaag gtagcgagtg cttcccacgg gtgctcacgc tgcggcgaga
     1621 ggagtggatt ctcaggatga cggcgtacga caaacatatt cttattatgc ggcagagaag
     1681 cgtagcggca caggcgttaa ccgcgccatc ttcttatctt tgagtttctt taggaactcc
     1741 gaaagcgaaa tttttgccgc acacaccaca gagtcggcgc cgccatagta cacggtgagc
     1801 gtgtcgccat gaacggtagc gccgcaggcg tacaccacgc ctgacttgaa cccttcgttc
     1861 tcgtaggtgg cgtccggcga gagaataggg aagggagaac gggcaataac cttggtaggg
     1921 tcagtgagat caaggagaag tgcaccgagc ttgtagcgtg atggttcgcg cgcgtccgtt
     1981 gcgtggtaga agaggagcca tccgtctttc gttttaatag gcggtgggcc agcaccacga
     2041 agcgtcgaat cccacacgct ctcatccttc acggggttgt aggggctttc tatggatgcg
     2101 ttctcgtcca aggtgtcgag atagtcgacg agtacacggt cacgtgagcc gctatgcaag
     2161 gagtggagta cggcaaactt gccgttaatc ttttccggga acaagaccca gttcttatgc
     2221 acctgtccta cgggtgaaag gtatgttggt gccgtccact tccagcgctt cgcgagtaag
     2281 tcactaatag cgatagaaga aacgccgata cgaagtgcgt cccatccgcc aaaggctgag
     2341 aaggtcatat acatgcggtc acctatgact accgcgcgtg ggtcttctac gcccgcaaac
     2401 gaaccgccag acttagagag tcctgagtgc tcggcaaagc gtcgggtggc atcgtcaccg
     2461 ccagcaagcg caaataccgg atagggaaga cgttcatcaa atgaagcacc attcttgctc
     2521 gaggcgtacc cgattcttga gacgccatcg ttacccaccg cgcgataaaa gagatgcacg
     2581 cgtccgcccg cctctattgc tgccgggttc atcacgcctc ccgcctccca gctttgtcct
     2641 cgtggtacca atataggatt gccgtgctca cgaagtaaac cgagtgagcg tgcgaattca
     2701 actatacgtt cccgtatcgc atgagtgcga atgagtttcc agagcgcata caagaaaagc
     2761 cccgtgc
//
LOCUS       DAORIN010000081         2753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_9705_length_2753_cov_4.930319, whole genome shotgun sequence.
ACCESSION   DAORIN010000081 DAORIN010000000
VERSION     DAORIN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9705_length_2753_cov_4.930319"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PK109_03200"
     CDS             <1..680
                     /locus_tag="PK109_03200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRH24567.1"
                     /translation="LYGITKWANEEGYDLAVHIHLNDTGAQNHQGFAVYVPDKEFGNA
                     EAARPLGEAIAFELNRFNASSTLPIENYGVVEDQSLIALGAFNTAEFPSVLVEYAYIY
                     EPKIKNVEARDLVLSDMAHQTYRGIEKALGATVSGSNTRALPYAWTGTTKAGISSPEA
                     YALQVALKSLGFFPPKGELLIGCPLSGVMDTCTVNALNAYQSSKGWTPTGAIGPMTKA
                     ALKAAGF"
     gene            771..1523
                     /locus_tag="PK109_03205"
     CDS             771..1523
                     /locus_tag="PK109_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24568.1"
                     /translation="MTIKKFLAGSVLALALLAVAPTTHAATSTSVQAQINLLLQMIAQ
                     LQTQLTALGGESVSGGTPTCTLTATPAAGELNDKVRVSWTSSNATKLSWVKDTSGKDT
                     LKVPTGNPKLSGSKNVKMTVLGNPKLTMKATGKDGTTSTCSVTIPVTEGDSDEDSSDD
                     KPVSCLLWADKTSYEYGDDITYSWSSRNASSAGWVIDTSGKDTLALPGDKLEANGTQS
                     VKASVIGNPTVTLKVRGSDGNGSCSTKVNITE"
     gene            1595..2725
                     /locus_tag="PK109_03210"
     CDS             1595..2725
                     /locus_tag="PK109_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRH24569.1"
                     /translation="MEPDKILAAYKKVGGRVETKGKVTLTSKEDFALFYTPGVGTVST
                     HLAAHPEDAKTLSVKGNSVAVISDGSAVLGLGNIGPYGALPVMEGKALIFKQFANIDA
                     WPIVLDTQDPEKIIEAVRAIAPGFGGINLEDIAAPACFEIEERLIKELSIPVMHDDQH
                     GTAIVVLAGLMNAAAVAGKDLKTMKLVIIGAGAAGAATALLLNEYGIHDVVVVDRKGT
                     IDASREDLTDVKQELARRTNPRHIQGALENALMGADAVIGLSGGGKLTSSHIETMAPN
                     PIVFALANPTPEILPEDAKKGGAAIVATGRSDFPNQINNALVFPGIFRGALDGNVTAI
                     TTTMKLKAAAALAALVSSPSADCIIPEVLDERVVPTIAAAVQ"
BASE COUNT          694 a          722 c          732 g          605 t
ORIGIN      
        1 gcctctatgg catcaccaag tgggccaacg aagaagggta tgaccttgcg gtgcatattc
       61 atttgaacga taccggcgca cagaaccacc aagggtttgc ggtgtacgta cctgataaag
      121 aattcgggaa tgcagaagcg gcacgcccgt taggggaagc tatcgcgttt gaactcaacc
      181 gtttcaatgc atcctccacg ctccccatag agaactatgg cgtggtggaa gatcagagcc
      241 ttatcgcgct cggtgccttc aatacggctg agtttccctc agtactcgtt gaatacgcct
      301 atatctatga gccaaagata aagaatgtgg aagcgcgcga cctcgtgctc tctgatatgg
      361 cacaccagac gtatcgcggt attgaaaaag cactcggcgc cacggtctcc gggagcaaca
      421 cgcgtgcact cccgtacgca tggactggta ctaccaaagc gggcatctcc agtcctgagg
      481 cctacgcgct tcaggtcgca ctgaagtcac tcgggttctt cccaccaaag ggagaactcc
      541 tcatcggctg cccactttca ggcgttatgg atacctgcac ggtgaatgcg ctaaacgcgt
      601 accaatcttc taaagggtgg accccaacag gtgcgatagg accaatgacg aaagcagcac
      661 tcaaagcagc agggttctag tggttgtgtg ggctatcata tttccagtgt tgaattatct
      721 ttgccgagag gtacagtaca ttggtcagta ttaatagact ctctctcact atgaccatta
      781 agaaattctt ggcaggaagt gtgctggcac tcgctctact tgcagttgct ccgactactc
      841 acgctgcgac aagtacctca gtgcaggcac agataaatct cttattgcag atgattgccc
      901 agcttcagac gcagctcact gcgcttgggg gcgagtcagt gtccggtggt acgcccacct
      961 gcaccctcac ggcaacgcct gccgcagggg agcttaacga caaagtgcgc gtgagctgga
     1021 cctcctcaaa cgcgaccaaa ctttcctggg tgaaagatac gtcgggcaag gataccctca
     1081 aggtgcccac tggaaacccg aagctttctg gttcaaagaa tgtaaaaatg accgtgctcg
     1141 gaaatccgaa gctcaccatg aaagctactg gaaaggatgg tacgacgagc acgtgctcgg
     1201 taaccatacc ggtgactgag ggagactctg atgaagactc ttcggacgat aagccagtgt
     1261 cttgtctact ttgggcggat aagaccagct atgagtacgg cgatgacatt acctattcct
     1321 ggtcgagccg gaacgcatcg tctgcgggat gggtcattga tacgtctggg aaagatactc
     1381 ttgcgttgcc gggtgataag ctcgaggcga acggcacaca atcggtgaag gcaagcgtaa
     1441 tagggaatcc gacggtaacg cttaaggtgc ggggttccga tggaaacggc tcgtgctcta
     1501 cgaaagtgaa tattaccgag taactacact cgcgtttacc gtaaaacgcc gtccccagtt
     1561 gggggcggcg ttttgctttg ctactatcag tccaatggaa ccagataaaa ttcttgcagc
     1621 atacaagaag gtagggggac gagttgaaac caaagggaaa gtaaccctta cctcaaaaga
     1681 agacttcgcc ctcttctata cgcctggagt aggcactgtc tcaacgcatc ttgccgctca
     1741 ccccgaagac gccaagacgc tttcagtgaa aggtaatagc gttgcggtca tctctgatgg
     1801 ctctgcagtg ttaggactcg gtaacatcgg gccgtatgga gcgcttcctg tgatggaagg
     1861 gaaggcactc atcttcaagc agttcgcgaa catcgacgcc tggcccattg tgttagatac
     1921 gcaggaccca gagaaaataa tagaggcggt ccgagcgata gcacccgggt tcggtggtat
     1981 taaccttgaa gacatcgcgg cccctgcctg ttttgaaatc gaagagcgcc ttatcaaaga
     2041 actttccatt cccgtcatgc atgacgatca gcatggtacc gccatcgtag tacttgcagg
     2101 actcatgaat gcagcggcgg ttgccggaaa agatttaaag acaatgaagc ttgtcattat
     2161 tggtgctgga gctgcgggtg ccgctaccgc actccttctg aacgaatacg gcatacacga
     2221 cgttgtagta gtggatcgca aaggcaccat cgacgcatca cgagaggatc tcactgatgt
     2281 gaagcaggag cttgcacgcc gcacaaaccc gcgccacata cagggagcgc ttgagaacgc
     2341 acttatgggt gctgacgcag tcattggtct ttcaggcggc ggcaaactca catcgagtca
     2401 tatagagacg atggcaccaa accctatcgt cttcgcgctt gcaaacccca caccagaaat
     2461 tcttcctgaa gatgcgaaga agggaggtgc agctatcgtt gctaccggac gctctgactt
     2521 cccgaatcaa atcaataacg cgctcgtgtt cccgggaata ttccgtggcg cgctcgatgg
     2581 aaacgtcact gccataacta ctactatgaa actgaaggcc gccgcagctc ttgccgcact
     2641 cgtgtcgtcg ccgtctgctg actgcatcat tcctgaagta cttgatgaac gcgtggtgcc
     2701 caccattgcg gcagcggtgc agtgatacca tacgcgcatg aatattcttg tct
//
LOCUS       DAORIN010000082         2752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_9712_length_2752_cov_5.275862, whole genome shotgun sequence.
ACCESSION   DAORIN010000082 DAORIN010000000
VERSION     DAORIN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9712_length_2752_cov_5.275862"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK109_03215"
     CDS             complement(<1..226)
                     /locus_tag="PK109_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HRH24570.1"
                     /translation="MDSRTAPQFDENAANSEPRWYTIHTYAGYENAVERNLKQRIESL
                     GMGNKIFEVVVPTEKKVRVKGGKRVVEEEKI"
     gene            complement(228..416)
                     /gene="secE"
                     /locus_tag="PK109_03220"
     CDS             complement(228..416)
                     /gene="secE"
                     /locus_tag="PK109_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRH24571.1"
                     /translation="MKSFISYLKNVRGEFAHIVWPKPRIALVHTGIIILISAIVAVFI
                     GLMDYALTSLVALIVNSY"
     gene            complement(541..1341)
                     /locus_tag="PK109_03225"
     CDS             complement(541..1341)
                     /locus_tag="PK109_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRH24572.1"
                     /translation="MNESFAETFLKATKPVRYAAAAALGVLALFLLALTINAFGNLGR
                     STNPYMNTITVEGTGKGTSIPNLAVISFSVSEQGATVAEAQGKATEKTDAALSAVSGL
                     AIDEKDVKTIAYNVYPRYENTAPCYSGYCPTSNPKIIGYEVSQTVEVKVRDTAKAGDV
                     LQALGTLNVQNISGPNFTVDEDDAIKNVAREEAIKEAKAKAKALADELGVSLGKVVSF
                     YETPAGGMPYYGYGGEAKAMDMAVQSAPSLPTGENETSITVSITYEIR"
     gene            1423..2187
                     /locus_tag="PK109_03230"
     CDS             1423..2187
                     /locus_tag="PK109_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRH24573.1"
                     /translation="MFEDPVIGSIIAVGAVSLVSLAGITTLSLRQDFLRKILFLLISL
                     AAGALLGDAFIHLLPEAFEEAGNPLVVSLVTLAGILSFFVLEKFLHWHHNHGDEEYSE
                     DHARIHPVGHLVLVSDGVHNLIDGLAIGAAFLVSPEVGIATAIAIALHEIPQEIGDFG
                     LLLHAGFSRTKALMLNFLSALTAFLGLGLAFWLSSLGDSLAPLIAAFAAGNFIYIALA
                     DLVPELQKTTGARRSTLQFFVIIAGLGAMVLLLGLE"
     gene            complement(2193..>2752)
                     /locus_tag="PK109_03235"
     CDS             complement(2193..>2752)
                     /locus_tag="PK109_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24574.1"
                     /translation="TMEAKVCPDGTSVGRSGPACEFTPCAFPNVEIASAKIAYALPAG
                     YVDGVQQPGADGENPGMLDFYQKQALGTSFHYLTVYAYPIPAGKTGNDVIIANTMFSP
                     SGEPATDMSKFKPVLVNGKTFQGVVIERFEGQVMSSYFLVRATDVLRFDIIEKDVDWT
                     NPDLVIENLPEHKALLKMLGTLQTQ"
BASE COUNT          571 a          756 c          707 g          718 t
ORIGIN      
        1 agatcttctc ttcttcaacg acgcgcttac cgcctttgac gcggaccttc ttttcagttg
       61 ggacgaccac ctcaaagatt ttgttaccca tgcccaagga ctcgatgcgc tgcttcaagt
      121 tacgctcaac ggcgttctcg tagcctgcgt aggtatggat ggtgtaccag cgtggttcgc
      181 tgttagcggc gttttcgtca aactggggag cagtgcggct atccatatta gtagctgtta
      241 acgataaggg cgacgaggct cgtaagcgcg tagtccatga gaccgataaa gacggcgaca
      301 atagcggaaa tgagaatgat gatgcccgtg tgcacaagcg cgatacgcgg cttaggccac
      361 acaatgtgag cgaactcgcc acgtacgttt ttgaggtagc tgatgaaaga tttcatgtcg
      421 ggtattttaa aacgcccctt cgggcgctct tgccggtgag tataccagaa agagcgggag
      481 cgtcaataat aacgaaaaac ggctccgtag agccgttttt ctcaaactat cctttcgata
      541 ctagcgaatc tcgtaggtaa tagagaccgt gatgctcgtt tcgttctcac ccgttggaag
      601 ggatggggcg ctctgtaccg ccatgtccat cgcctttgcc tctcccccat agccgtagta
      661 cggcatgccg ccagcaggag tctcatagaa ggagacgacc ttgccgaggg agaccccaag
      721 ttcgtcagca agcgccttgg cttttgcctt cgcttctttg attgcctctt cacgtgcgac
      781 gttcttaata gcgtcatctt cgtccacggt gaagtttggt ccggagatgt tctgcacgtt
      841 caacgtacca agtgcctgaa gcacatcgcc cgcctttgcc gtatcgcgca ctttaacttc
      901 tacggtctga gaaacctcgt acccgatgat ctttgggttt gaggttgggc agtacccgga
      961 gtagcagggt gcggtgttct cgtaacgagg atagacgttg tacgcaatgg tcttcacgtc
     1021 tttttcgtcg atagcaagtc cagaaacggc agaaagagca gcatccgtct tttcggtagc
     1081 ctttccctgc gcttcagcaa cagttgctcc ctgttcgctt acggagaaag aaatgacggc
     1141 aaggttcggg atagaggtcc ccttccccgt accttcaacc gtaatggtgt tcatgtacgg
     1201 attggttgag cgcccaagat taccaaacgc gttaatggtg agcgcgagca agaagagcgc
     1261 gaggacgccg agtgctgctg cggcagcata gcggaccggc ttcgtggcct tcaaaaacgt
     1321 ttctgcgaag gattcattca tacgaatgtg attatttatt tgtgggcgta ctatatcacg
     1381 acgcatttcc cctccgtttg tgacagtgta tgctttacga atatgtttga agaccccgtc
     1441 attggaagca ttattgccgt gggcgccgtc agtctcgtgt cgcttgccgg catcaccacc
     1501 ctctcgcttc gccaagattt tctccgaaag atcctcttcc tcctcatctc acttgctgca
     1561 ggtgccctct tgggtgacgc ctttatccac cttctccccg aagcatttga ggaagcaggt
     1621 aaccccctcg tggtgtcgct cgtaaccctt gccggcatcc tttccttctt cgtgcttgag
     1681 aagttccttc actggcatca caaccacggg gacgaagagt actcagaaga tcacgcacgc
     1741 atacaccctg tcggccacct cgtattggta tctgacggcg tccacaacct tattgatgga
     1801 cttgcgatag gggcagcgtt tctcgtttct cccgaggtgg gtattgctac cgctatcgct
     1861 atagcgctcc atgagattcc tcaagagatc ggcgacttcg gtcttctctt gcatgctggg
     1921 ttctcgcgca cgaaggcgct catgttgaac ttcctttcag cactcacggc attccttggg
     1981 cttggacttg cgttctggct ttcctcgctt ggagactccc ttgctccact tatcgctgcc
     2041 tttgctgccg gtaactttat ctacatcgca ctcgccgacc ttgtgcctga gttacagaaa
     2101 accaccgggg cgcgccgttc tactcttcag ttctttgtca ttatcgccgg ccttggggcg
     2161 atggtgcttc ttctcgggct cgagtaaaac aactactgcg tctgtaacgt accgagcatc
     2221 tttaggagtg ctttgtgctc gggaagattt tcaataacga gatccgggtt cgtccaatca
     2281 acatccttct cgatgatatc gaagcgaagc acgtccgttg cgcgcacgag gaaatacgaa
     2341 ctcatcacct gcccttcaaa gcgttcaata actactccct ggaacgtctt accgttcacg
     2401 agtactggct tgaacttact catatctgtc gccggttctc ctgaaggaga gaacatcgtg
     2461 ttcgcgataa tgacgtcatt ccccgtcttc cctgctggaa tggggtacgc atacacggtg
     2521 aggtagtgga aagaagtacc gagtgcctgc ttttgataga agtcgagcat gccaggattt
     2581 tccccgtcag cgcctggttg ctggacaccg tccacatatc cggcaggaag cgcgtaggca
     2641 atcttcgcgc tcgcaatctc cacgttcggg aatgcgcagg gagtgaattc acacgcaggg
     2701 ccgctgcggc caacggatgt cccatccgga catacctttg cttccatggt gc
//
LOCUS       DAORIN010000083         2705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_10012_length_2705_cov_4.961887, whole genome shotgun sequence.
ACCESSION   DAORIN010000083 DAORIN010000000
VERSION     DAORIN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10012_length_2705_cov_4.961887"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..488
                     /locus_tag="PK109_03240"
     CDS             192..488
                     /locus_tag="PK109_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24575.1"
                     /translation="MGENLDTRVGEALAALAKDGGNVSSPVLLVQVVLDDQIGSAVMR
                     DKNDLEDALETALYKWAAGGRSEHAGPAMIIVSAIKGTRSGDAYHYVHLSDYYI"
     gene            complement(542..703)
                     /locus_tag="PK109_03245"
     CDS             complement(542..703)
                     /locus_tag="PK109_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HRH24576.1"
                     /translation="MLLTIAVLLIILWGLGLVTSYTLGGFIHLLLLVAIVMVLIRIIQ
                     GKNPISGGG"
     gene            complement(765..929)
                     /locus_tag="PK109_03250"
     CDS             complement(765..929)
                     /locus_tag="PK109_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HRH24577.1"
                     /translation="MLLILAALLIILWAIGFATSFVLGGFIHLLLIAAAILIIMRMFD
                     GKKQIPGSSL"
     gene            complement(1037..1345)
                     /locus_tag="PK109_03255"
     CDS             complement(1037..1345)
                     /locus_tag="PK109_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24578.1"
                     /translation="MVHFRDLSEHDTFVIAGINTQRPFHIRGNGELYILRDITTKFEW
                     TLVSPESKRRIEEANPPVVLVQLDPTGCIELAFARSNCPSYKQRLDQQVEQALKGAET
                     "
     gene            complement(1406..>2705)
                     /locus_tag="PK109_03260"
     CDS             complement(1406..>2705)
                     /locus_tag="PK109_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRH24579.1"
                     /translation="NDMPSNGGASRDLTPLLDVILEHVPPASSPELVAKPLKLQAFNL
                     AYDNFLGRLAICRVYEGAVHPSENVFEKKPDGTKSSGKLTKIFTFKGLERVEAAVAEA
                     GDIVLIAGLPDVYIGDTITTDENAEPLPAIAIDEPTITVNFLVNNSPFAGKEGKYVTS
                     RQLRDYLERELQVNVGLRVDFTSPESFKVAGRGELHIAILLENMRRAGYEMQVSQPQV
                     IIREEDGKKLEPFEEVIIDAPSEFQGAIIEKLGTRAFVLQNLTQHDNLVRLTLEGPTR
                     GLLGYKNVFVVDTKGEGILSSRVIGFKPYAGEIKKRQVGSMISMTAGKSLGYALWYLQ
                     ERGVLYINPATEVYEGMVVGNTSKGEEMMVNPTKAKNQSNVRSSGMDEAINLFPPILL
                     TIERGLELMSEDEYLEITPESVRLRKQFLTEQDRAKNRRA"
BASE COUNT          642 a          661 c          728 g          674 t
ORIGIN      
        1 aatctcaaga cggaaaaccg atagaaaact gttgtgccat taatatagca cctccaagca
       61 tatgtggcaa gtcttcctgg taggctggga agtgtctgtt agtgtagtac tacactgctt
      121 actgaaatcc cttcggtttg cagtggttca ggagaacgac gatggcaaag tttaatgcca
      181 atgctcttat agtgggcgaa aacctggaca cacgtgtcgg cgaagcactc gctgccctcg
      241 ccaaagatgg tggcaatgtt tcaagccccg tactgttggt gcaagtggtc ttagacgatc
      301 agataggttc cgccgtcatg cgggataaga acgacctcga agatgcgctt gaaaccgctc
      361 tttacaaatg ggctgccggg ggccgttccg aacatgcagg cccggcgatg atcattgtct
      421 ctgcgatcaa agggacgagg tcaggagacg cctatcatta tgtgcatctc tctgattact
      481 acatctagat cgtcagatct ttttgttctt tcgcgcgcct tatttgagag gcgcgcactt
      541 tttaacctcc tcctgaaata ggattctttc cctgaataat acgaatgagt accatcacta
      601 tcgctactaa gaggagcaag tgtatgaatc caccgagcgt gtagctcgtg acgaggccga
      661 gaccccagag gataataagg aggacagcga tggtaagaag cataggaaag taatttgtta
      721 ataaccttgc tgttataccc tccctcgtat gaagcagggg tgaattagag agacgatcct
      781 ggtatctgct ttttaccgtc aaacatacgc attatgataa ggatagcggc ggcgataagg
      841 aggagatgaa tgaatccacc gagcacaaac gaggtagcga atcctatagc ccaaagaatg
      901 ataagcaagg cagcgaggat gagcagcatg ggtaggataa aagaattact gtggtaatac
      961 ccctttgtac acttctagtg gtgaaagccg catgaatgtg cgaagaccgc catgtgggcg
     1021 gtcttgctgt cggtgattac gtttcggctc ccttcaacgc ctgctccacc tgctgatcga
     1081 gccgctgctt gtatgacggg cagttgctac gtgcgaaggc cagctcgatg catcctgtcg
     1141 gatccagttg cacgagcacc acaggaggat tcgcctcttc gatacgtctc ttgctctcag
     1201 gagacactag cgtccattcg aatttggtag tgatgtccct aaggatatat agttcaccgt
     1261 tacctcggat gtggaaggga cgctgcgtat tgataccggc aatcacgaag gtatcgtgtt
     1321 cagacagatc acggaaatga accatttcat ttctcctttc tccgcgctga atcttacgag
     1381 tttcagcctc tcctgacaat ggggtctatg cgcggcggtt tttcgctcga tcctgttcgg
     1441 tcaaaaactg ctttcttagg cgtactgatt ctggagtaat ctcaagatac tcatcttcgc
     1501 tcatgagctc gagaccgcgc tcaatagtga ggaggattgg ggggaagagg ttaatcgcct
     1561 catccatacc ggacgaacgg acgttcgact ggttctttgc tttggttgga ttcaccatca
     1621 tctcttcacc cttagacgtg ttacccacca ccataccttc gtacacttcc gttgcaggat
     1681 tgatgtagag aacgccgcgt tcctggaggt accagagggc atagccaagc gacttaccag
     1741 cagtcattga gatcattgaa cctacctgac gcttcttgat ttcaccggca tatggtttga
     1801 agccgataac gcgtgaggag agaatacctt cacccttcgt atcaacgacg aacacgttct
     1861 tgtatccgag gagaccacgc gttggtcctt caagcgtgag gcgcacgagg ttgtcatgct
     1921 gcgtgagatt ctggagcacg aatgcgcgcg taccaagctt ctcgatgata gcgccctgga
     1981 actcggaagg agcatcgatg atgacttctt cgaatggctc gagcttcttg ccgtcctctt
     2041 cacggatgat gacctgcggc tgggagacct gcatttcgta tcctgcgcga cgcatgttct
     2101 caagcaagat agcgatgtgg agctcgccac gacccgcaac cttgaatgat tctggggacg
     2161 tgaagtcaac gcgaagcccg acgtttacct ggagctcgcg ctcgagatag tcacggagct
     2221 gacggctcgt cacatacttc ccttccttac ccgcaaacgg tgagttgttc acgaggaagt
     2281 tcaccgtgat ggttggctca tcaatggcga tagctgggag tggctcagca ttttcgtccg
     2341 tagtgatggt gtcgccgatg tagacgtcag gaagtcctgc gatgagaacg atgtcgcctg
     2401 cttcagctac cgcagcttca acgcgctcaa gacccttgaa ggtaaagatc ttggtgagct
     2461 ttccagagct cttcgtgcca tccggtttct tttcaaatac gttttcagat gggtgcaccg
     2521 cgccttcgta tacgcggcag atagcaaggc ggccaaggaa gttgtcatac gcgaggttga
     2581 atgcctggag cttcaatggt ttagcaacga gttctggaga ggatgcgggt gggacgtgct
     2641 cgagaataac gtcgagaagc ggcgtgaggt cacgggacgc tccgccgttg cttggcatat
     2701 cgttt
//
LOCUS       DAORIN010000084         2622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_10630_length_2622_cov_5.534866, whole genome shotgun sequence.
ACCESSION   DAORIN010000084 DAORIN010000000
VERSION     DAORIN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10630_length_2622_cov_5.534866"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PK109_03265"
     CDS             <1..923
                     /locus_tag="PK109_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRH24580.1"
                     /translation="LVRMITRHEREGLTWVDLESPTRDELRSVVEEFSIDAQVEEEII
                     SPTPYPLFVASSEYAYLILHFPTAEVSGGARNQEIDIIVGKNFLITARYEVVGCILTL
                     HKAFEAEELLGLPTEGGCAALVERLLRRLYGAMGEEVERVARSLDRIEADIFAGKERT
                     TVRAISEAGRILLRFDTTLTRHSEPLTAFLEELKANFFGKKFLKEATRIEAERAHAAS
                     LVASYRAVARELRTTNDSLLSSSQNEVMKIFTMMTVAFLPLTFIAGLFGMHAKYPPIL
                     GHQHDFALIVIFMVIVELLLILYLRVKKWI"
     gene            927..2369
                     /gene="cysS"
                     /locus_tag="PK109_03270"
     CDS             927..2369
                     /gene="cysS"
                     /locus_tag="PK109_03270"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRH24581.1"
                     /translation="MWPFGSNKRAQVFFTDTLSGEKKEFVPITKGKVRLYSCGPTVYG
                     AIHIGNLRSFVLADLVARTLKASGYKVERVMNITDVGHMVGDGDAGDDKLAVGAAREK
                     TTPKAVADKYTKLFMEDIKALGIDTSDFKKFPRATSYIQEDIEMIKALEKKGYTYLTS
                     EGLYFDTTKFPNYGSLLGMKDAQLRAGVRVDMGEKKSPHDFVLWRTAKSNDLQKWNSP
                     WGEGNPGWSIECSAMATALLGTQIDIHTGGEDLASVHHNNEIAQSEAASGKHPFSRYW
                     IHGAFLTMNGEKLAKSAGNSFTLADVVARGIHPLALRYLFLQAHYRSPLSFSWDALAA
                     ADTTLQRLWKETSAIKAKVRGASKKTKAIELINEAAYDDLATPQVIAQLWMVINDTDL
                     SSEEKWAVIEAVEKLLGLSLTIPPRTTLSDAVALSDLPEEIRALATGREEARKNKDFA
                     KSDELRIHLSERGYAVEDTPSGTTYTKIGG"
     gene            2384..>2622
                     /locus_tag="PK109_03275"
     CDS             2384..>2622
                     /locus_tag="PK109_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HRH24582.1"
                     /translation="MADPTLRVPPQSLESERGLLGALLLKPDVIHDVGDTVRGESFYA
                     EKHKIIFEAMHELSGKGEPIDLLSLSERLQALGHL"
BASE COUNT          638 a          706 c          665 g          613 t
ORIGIN      
        1 tactagtgcg tatgattacc cgacatgagc gcgaaggcct cacgtgggtg gacctcgagt
       61 ccccgacgcg cgacgaactc cgttctgtcg tcgaagagtt cagtattgat gcgcaggtag
      121 aggaagaaat tatctcgccg accccgtacc cactcttcgt tgcctcaagt gagtacgcct
      181 atctcattct tcacttccct accgcagagg tttcaggtgg cgctaggaac caggaaatag
      241 acattatcgt tggaaagaac ttccttatta cggcccgcta tgaggtggtg ggctgcatcc
      301 ttacgctcca caaggcgttt gaggcagagg aacttcttgg cctccctacc gaaggcggat
      361 gcgccgctct cgttgaacgt ctattacgcc gcctctatgg cgcgatgggt gaagaagttg
      421 agcgcgtcgc acgttccctt gatcgcattg aggcagatat ctttgctggc aaagaacgca
      481 ccaccgtgcg tgctatctcg gaagcaggcc gcatccttct tcgctttgac actaccctta
      541 cccgtcacag cgaaccgctg accgcgttcc ttgaagaact caaagctaac ttctttggaa
      601 aaaagttctt aaaggaagcc actcgcatag aagcggagcg cgctcatgcc gcttctcttg
      661 ttgcgtcgta tcgtgccgta gcacgtgagc ttcgcaccac caacgactca ctcctttcat
      721 cgagtcagaa cgaagtgatg aagatcttta cgatgatgac cgttgccttc ctcccgctca
      781 ccttcattgc tggcctcttt ggtatgcatg ctaagtatcc ccctatcctc ggtcaccagc
      841 atgacttcgc tcttatcgtc atattcatgg ttatcgtaga gctgttgctc atcctctatc
      901 tacgcgttaa aaagtggata taactcatgt ggcctttcgg tagcaacaaa cgcgcgcagg
      961 tcttctttac ggacacgctt tcaggtgaaa agaaggagtt cgttcctatt actaaaggaa
     1021 aggtgcgttt gtattcctgc ggtcccaccg tctatggcgc tatccatatc ggtaacctcc
     1081 gttcctttgt attagcggat cttgttgccc gcaccctcaa ggcaagcgga tacaaggtag
     1141 agcgagtgat gaacatcacc gacgtcgggc atatggtcgg cgacggcgac gcaggagacg
     1201 acaagcttgc cgtgggtgct gcgcgtgaga agacgacgcc taaggcggta gcggataagt
     1261 acacgaagct ttttatggag gacatcaagg cgctcggtat tgatacaagt gacttcaaga
     1321 agttcccccg cgcaacgagc tatatacagg aagacatcga gatgatcaaa gcgctcgaga
     1381 agaaaggtta tacctatctc acttccgaag gtctctactt tgataccact aagttcccga
     1441 actacggttc tctgcttggc atgaaagatg cgcaactgcg cgcaggcgta cgcgttgata
     1501 tgggtgaaaa gaaatctccc catgactttg tgctctggag gacagcaaaa tcgaatgatc
     1561 ttcagaaatg gaattcaccg tggggcgagg gtaaccccgg ttggagcatt gagtgctcgg
     1621 caatggcgac ggcgcttctc ggtacgcaaa ttgatattca taccggaggc gaagaccttg
     1681 caagcgtgca tcacaacaac gagatagcgc agtcagaagc agcgtcaggg aaacatccgt
     1741 tttctcgcta ctggatacac ggcgcgttcc tcactatgaa cggtgagaag ctcgcaaagt
     1801 ctgccggtaa ttcgtttacg cttgctgatg tagtggcacg agggatacat ccactcgcat
     1861 tgcgctacct gttccttcaa gcgcattacc gctctcccct ttcattctcc tgggacgcgc
     1921 ttgctgcagc cgacaccacg ttacagcgtc tttggaagga aacgagtgcg ataaaagcaa
     1981 aagtacgtgg cgcaagtaag aaaacgaaag ctatcgaatt aatcaatgaa gctgcatatg
     2041 acgatttggc tacgccgcaa gtaatcgctc agctctggat ggttatcaat gacacagacc
     2101 tctcaagcga agaaaagtgg gcggtcatag aggctgtcga aaaactactc ggtctctccc
     2161 tcactattcc tccccgtacc acccttagtg acgcagtagc gctcagcgac ctccctgaag
     2221 aaattcgtgc cttagccaca gggcgcgaag aagctcgtaa aaacaaggat tttgccaaat
     2281 ctgacgagct acgtatccac ctttcggaac ggggatatgc tgtggaagac accccttctg
     2341 ggactacata cacgaaaata ggagggtgat aaactgaggg tccatggccg acccaacgct
     2401 ccgcgtcccc ccacaaagtc tcgaaagcga gcgcggactt cttggtgccc tcctcctaaa
     2461 acctgacgtc attcatgacg tcggagacac tgtacgcggc gagtccttct atgcggagaa
     2521 gcataagatc attttcgaag cgatgcatga gctctccggt aagggtgagc ctatcgatct
     2581 cctctccctc tccgagcgcc ttcaggcact tgggcatttg ga
//
LOCUS       DAORIN010000085         2463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_11912_length_2463_cov_4.492940, whole genome shotgun sequence.
ACCESSION   DAORIN010000085 DAORIN010000000
VERSION     DAORIN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11912_length_2463_cov_4.492940"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PK109_03280"
     CDS             complement(<1..495)
                     /locus_tag="PK109_03280"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRH24583.1"
                     /translation="MALTPPALLEALLFASGEAVSKKRLATLLEVTPALLDQAADVLR
                     GQLAGRGLALIETENHIELRTAADAADFVKKLREAELSRDLGKAGLETLALILYRGSA
                     TRGEIDWVRGVNSSATVRSLLLRGLIEKTEDSEDKRRPRYKPTVDALAHLGVEKAEEL
                     PAYAE"
     gene            complement(583..1326)
                     /locus_tag="PK109_03285"
     CDS             complement(583..1326)
                     /locus_tag="PK109_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRH24584.1"
                     /translation="MEVLTVHNNNYTVRTPAFEGPFALLLELIEKRKLSVSDLSLSQV
                     TDDFIQHVRSQAAFPMEDAAEFIQVAATLLLIKSRSLIPDLELSGEEEQDVDDLKRRL
                     VAYERAREAAKQLGRLFGRSVMVSAGERTPDVVFAPSKDLTLQGLEAALRSALAELEK
                     EEQLPAVRVKPMVTIEEMMDRLLERVQSAITMSFKDFSGDAKEKVEVIVSFLALLELV
                     KQGAVDVAQHETFSDIRITNTSTATPKYG"
     gene            complement(1330..2292)
                     /locus_tag="PK109_03290"
     CDS             complement(1330..2292)
                     /locus_tag="PK109_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HRH24585.1"
                     /translation="MPTLKELYTIAVEAGIDADPRGRDGVEKMLARRKKEYEALSETK
                     KKEYDQEDLWDPYIDSGILAGNPDGEVKKVMAGIDIDVGEVAIAGILNDRGASIDALV
                     AHHPQATGYAALHEVMEMQSENLARYGVPINIGEGVMRDRIGEVARKISPRNHTQAVD
                     AAKLLGLSFMATHTMADNMVFDFLEKLFIERSAETVGDVMSILKEIPEYQQAMKVKAG
                     PMIFSGSESNKTGKIAPLEITGGTEGSHLIYEKLAHAGVGTIIGMHASEEHRKEAQKY
                     HLNLVIAGHMSSDSIGMNLILDKFEAKGVEIVPVSGFIRVSRNK"
BASE COUNT          552 a          656 c          621 g          634 t
ORIGIN      
        1 ctctgcatac gcaggaagct cctctgcctt ctccacgcca agatgcgcga gcgcatctac
       61 cgtcggctta tagcggggac gccgtttatc ctcagagtct tccgtcttct cgataagacc
      121 acgcagaagg agtgaacgca cggtagcgga cgagttcacg ccgcgtaccc aatcgatttc
      181 gccgcgcgtt gcacttccgc gataaagaat aagagcaagg gtctcaagcc ctgctttccc
      241 gaggtctctc gataactctg cttcacgaag cttttttaca aagtcagcag cgtccgctgc
      301 agtgcgtagt tcaatatggt tctctgtttc aataagtgcg agaccccgac ctgcaagctg
      361 accgcgtaga acgtcagctg cctgatcgag aagtgcaggg gtaacttcaa gtagtgtggc
      421 aagtcgtttc ttagacaccg cttcgccgga cgcgaagagc agtgcctcaa ggagtgctgg
      481 aggagtaaga gccataccta ttcatacaga ataacgctta tatacggcag ggccaacctt
      541 gacgggtggg gagaaatacc gtggaagaaa taaaaccgat ctctacccgt actttggcgt
      601 agcggtggaa gtattggtga tgcgaatgtc agaaaaggtt tcgtgctgcg ccacgtccac
      661 tgctccctgc ttcacgagtt caaggagcgc gaggaacgaa acgataacct ccactttctc
      721 tttggcatct cctgagaagt ctttaaatga catcgtgatc gcactctgta cacgttcaag
      781 gagtctatcc atcatttctt cgatggtgac cattggtttt acgcgtactg cgggaagctg
      841 ttcttccttt tcaagctcag ccaaggcact gcggagcgcc gcttctagtc cttgaagcgt
      901 gaggtctttg gagggtgcga atactacgtc aggcgtgcgc tcccctgctg acaccatgac
      961 tgaacgcccg aaaagacgtc caagctgctt ggctgcttcg cgagcgcgct cgtacgcgac
     1021 gaggcgacgc tttaaatcat caacatcctg ttcctcttct cctgagagct ctagatcagg
     1081 aatgagagaa cgtgacttaa taagcaacaa ggttgccgct acctgaataa actctgcggc
     1141 gtcttccatt ggaaacgccg cctggcttcg tacatgctgg atgaagtcgt cggtgacctg
     1201 cgaaagagaa agatcggaaa ccgaaagctt tcgcttctcg ataagttcca agagtagcgc
     1261 aaagggtcct tcaaaggctg gagtgcggac cgtgtagtta ttgttgtgga cggtcaggac
     1321 ttccatagat tacttgttcc gacttacgcg gataaaaccg gaaaccggaa cgatttcaac
     1381 acccttggct tcgaacttat cgaggatgag gttcataccg atggagtcgg aactcatgtg
     1441 acctgcgata acgagattta aatgatactt ctgtgcctcc ttgcgatgtt cttctgaggc
     1501 atgcatgcca atgatagtgc cgacccctgc gtgcgcgagc ttctcataga taagatgcga
     1561 gccctcggtg ccgcccgtga tttccaatgg tgcgatcttg ccagttttat tgctctcgct
     1621 tccggagaaa atcattggac ccgcctttac tttcattgct tgctggtatt caggaatttc
     1681 cttaagaata ctcatgacat ctcctacggt ttctgctgaa cgctcgatga aaagcttctc
     1741 aagaaaatca aagaccatgt tatcagccat ggtgtgcgtc gccatgaagg aaaggccaag
     1801 gagctttgca gcgtctactg cctgcgtatg gtttcgcgga ctgatttttc gcgcgacttc
     1861 tccaatgcgg tcgcgcatca cgccttcgcc gatatttatt ggcactccgt agcgagcaag
     1921 gttttccgat tgcatctcca taacctcatg gagtgctgca tacccggttg cctgagggtg
     1981 atgagcaacg agcgcatcga ttgaagcacc acggtcgttt aagataccgg caatggcaac
     2041 ttctccgaca tcgatatcaa taccggccat gaccttcttc acctctccgt ctggattccc
     2101 tgcaagaatt cctgagtcta tatagggatc ccaaaggtcc tcttggtcgt attctttttt
     2161 cttcgtctcc gaaagcgcct catattcctt cttacggcgc gcaagcatct tctcgacgcc
     2221 gtcgcgtccg cgcgggtctg cgtctatgcc ggcttcaacg gcgatggtgt aaagctcctt
     2281 gagtgtaggc atacgctaat tttttttctg ctcctttacc gcaattccaa gctcatcgag
     2341 ctgcttcttc tcaagcggct tcggtgcctt catcactgag gtttgtccgc cacgcgtcat
     2401 cgggaaggct tgtacctcgc gtaggtaggt ctcaccagtg aggttcatga tgttgcgttc
     2461 aac
//
LOCUS       DAORIN010000086         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_11991_length_2454_cov_3.958733, whole genome shotgun sequence.
ACCESSION   DAORIN010000086 DAORIN010000000
VERSION     DAORIN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11991_length_2454_cov_3.958733"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PK109_03295"
     CDS             complement(<1..913)
                     /locus_tag="PK109_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24586.1"
                     /translation="MRTVALILLGSFLLLTPFFAASTHAQGIPTTVNELSLSISPQYP
                     RPYDTVTVTVGSTQLDLASGIISITVNGKVVSEGERSATFTLGGPGVKTTIQATVKDA
                     LGTHSVSRTITPADVALVLEPTSTVPPFYKGAKLVASEGSLRLIALPNFQTASGTAIS
                     PQSLSYTWKFGDKILEAQSGVGKNVLVATAPVRYRDARITVTVTNADKSLVAQATTLI
                     SPVDPVIRIYKNDPLAGVDFSSALSGSFVLPSDEETFRAVPYYYASTPTLAWTLNGSD
                     SGSDADLTVRTTGATKGTALLGVRAAVM"
     gene            complement(965..1603)
                     /locus_tag="PK109_03300"
     CDS             complement(965..1603)
                     /locus_tag="PK109_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24587.1"
                     /translation="MPFSKELAALKTLQNDPAYGDGGGKQRIHVVIAFAMVATALLID
                     IVQILFEWLPAVGPIIGWFFAAFGFIVFGIWFMLVGVNYLDRNGAVKVMVSLASCVVE
                     LIPFVDMIPALTLGVLSLILITRAEDGKPSMLFGLAARRQAAMKPEELAGARQRADAT
                     LERDAKSNASRMVGGSQPLPRQRAGNYQAPTLRDYERDVQDNPSPEEVKEAA"
     gene            complement(1607..>2454)
                     /locus_tag="PK109_03305"
     CDS             complement(1607..>2454)
                     /locus_tag="PK109_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HRH24588.1"
                     /translation="EDENPSDILRSNIINLVGLFRIMLGGLTPEEDAIVDRAITETYA
                     LKDITGDADFTGVEAPLLSDFENVLSGMQGSESLVQRLSKYTKGTWSGFLNQPTNVDI
                     NKQFVVFSVRDMEDELKPVAMYIVTHFIWNAVRHELKKRLLIIDEAWWMMKSEDTASF
                     LYSLAKRGRKYYLGVSTITQDVEDFLRSPYGKPILTNSSIQLLLKQSPTTIDLLKDIF
                     NLTDEEKFLLLESGVGEGLFFAGLKHVAIKIVASYTEDQIITSDPSQLLAIKREKDRL
                     ETGGQ"
BASE COUNT          572 a          647 c          664 g          571 t
ORIGIN      
        1 ccataaccgc tgcgcgtacc ccaagaagcg cggttccctt ggtggccccg gtggtgcgta
       61 ccgtgaggtc ggcatcgctc ccactgtcac ttccgtttaa ggtccacgcc aaggtcgggg
      121 tcgatgcgta atagtacgga accgcacgga aggtttcttc atcgctcgga agcacaaatg
      181 agccagaaag ggctgaggag aaatcgactc ccgcaagcgg atcgttctta tatatacgga
      241 taacggggtc cacaggactt atgagtgtcg ttgcctgcgc gacgagcgac ttgtcggcgt
      301 tggtgacggt gaccgtgatg cgagcgtcac ggtagcgtac aggggccgtg gcaacaagga
      361 cgttcttccc tacccctgac tgtgcttcaa gtatcttgtc accgaacttc caggtatagg
      421 aaaggctttg gggcgatatg gcagtgccgc ttgcagtttg gaagtttgga agcgcgatga
      481 gacggagact tccttcagac gctaccaact ttgctccctt ataaaacgga gggaccgttg
      541 aggtgggctc tagtaccagt gccacatcag cgggggtaat agttctcgac acgctatggg
      601 ttccaagagc atctttaacg gtggcctgta tggtggtttt gacccccggg cctccaaggg
      661 taaaggtagc gcttcgttct ccttcagaca ccacctttcc atttaccgtg atgcttatga
      721 tgcccgacgc taaatcaagc tgagtgctcc ctaccgtcac ggtgaccgta tcgtatgggc
      781 gcgggtactg cgggcttatg gaaagcgaaa gctcatttac tgtggtagga attccctgcg
      841 cgtgggtgct tgccgcaaaa aacggagtga gcaacaaaaa agaaccaagg agaataagcg
      901 ctacggtacg cataggcaca gtatagcgtg tccctagacg ctcgacggat gcacgtgggt
      961 attcttatgc cgcttctttt acttcttcag gacttggatt gtcttgcacg tcacgctcat
     1021 aatctctcaa cgtaggcgcc tggtagttac cagcacgctg tcgaggtagt ggttgtgacc
     1081 ctcccaccat gcgagacgca ttacttttcg catcacgctc cagggttgca tctgcgcgct
     1141 ggcgcgcccc cgcaagctct tccggcttca tagcggcctg acgtcgtgcg gcgaggccaa
     1201 acagcattga cggcttcccg tcctcagcgc gcgtaataag aatgagggag agcactccaa
     1261 gcgtaagagc gggaatcatg tctacgaacg ggataagctc cacgacacat gaggcaagac
     1321 tcaccattac tttcactgcc ccgttccgat caagatagtt aacgcccacc aacataaacc
     1381 aaataccgaa cacgatgaac ccgaacgctg cgaaaaacca cccaatgatt ggccccacag
     1441 cgggcagcca ctcaaaaagt atctggacga tatcaatgag aagagcggta gccaccatcg
     1501 caaacgcgat gaccacatgt atccgctgct ttccgcctcc gtcaccgtag gctggatcgt
     1561 tctgaagtgt ttttaatgcg gcaagctctt tagaaaaagg catggattac tgtccgcccg
     1621 tctcaagtcg atccttttcg cgcttaatcg caaggagctg agacggatct gaggtgatga
     1681 tttgatcttc ggtgtaggac gccacgatct tgatagcgac gtgctttagg cccgcgaaga
     1741 agagcccctc ccccactcct gactcgagga gtaaaaactt ctcttcgtcc gtgagattga
     1801 agatatcttt tagaagatcg atagtcgtcg gcgattgttt caggagcaac tgaatagacg
     1861 agttcgtgag gataggtttt ccgtacggac ttcgcaagaa gtcttccacg tcctgggtaa
     1921 tggtggaaac cccgagatag tatttgcggc cacgctttgc gagtgagtag aggaaggatg
     1981 cggtgtcttc cgacttcatc atccaccatg cctcatcgat gataaggaga cgcttcttga
     2041 gttcgtggcg taccgcgttc catatgaagt gcgtcacgat gtacatcgct accggcttca
     2101 actcgtcctc catatcgcgt acggagaaca ccacaaactg cttattaata tcaacgttcg
     2161 ttggctgatt gaggaatcca ctccacgtac ctttggtgta cttcgaaagg cgctgtacca
     2221 aggactccga gccctgcatg cccgagagta cgttttcaaa gtcagagaga agtggtgcct
     2281 ctacccccgt gaagtcagcg tcgccggtga tgtctttcaa ggcataggtt tcagtaatgg
     2341 cgcgatcaac gatagcgtcc tcttcaggcg tgaggccgcc aagcatgatg cggaagagtc
     2401 ccaccaggtt aatgatgttt gaacggagga tgtctgaagg gttctcatct tctt
//
LOCUS       DAORIN010000087         2438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_12141_length_2438_cov_4.407889, whole genome shotgun sequence.
ACCESSION   DAORIN010000087 DAORIN010000000
VERSION     DAORIN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12141_length_2438_cov_4.407889"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK109_03310"
     CDS             complement(<1..568)
                     /locus_tag="PK109_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRH24589.1"
                     /translation="MALSSKTVRIGAALLVSALFVFAGFYFSSPFQLTIAGATSTDEL
                     LKEYAKKDTDGDNLPDWQESLYGTDPSNPHSVEAGLTDSEAVAAGKVALRFKSETPTA
                     PEPLTDDDFEVPAPTPGSATDEFAKSFFETYVTTGKGGTGDNSALVASLVSEAKTRVA
                     AQVSTSYQIASVHSVPGTSVSSYAAAIEA"
     gene            complement(623..1399)
                     /gene="rsmA"
                     /locus_tag="PK109_03315"
     CDS             complement(623..1399)
                     /gene="rsmA"
                     /locus_tag="PK109_03315"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRH24590.1"
                     /translation="MRAKKSLGQNFLMHARIAERIADAALLTANDTVLEIGPGTGMLT
                     RPLLARARRVVAVEADHTLIATLEETFATEIKEKKLTLVYGDIRAFDPASIGEPYALV
                     ANIPYYITGEIIRSFLGATHAPFTMTLLVQKEVAERIARSKKESILSLAVKVYGDPKY
                     SFTVPRGAFLPAPNVDSAVLTITNIKNPFSSKNTETFFFDLLHAGFAHKRKLLIRNLE
                     AVADASLLTTAFAEASISEKARAEDLSLATWKKLAQLLGG"
     gene            complement(1401..>2438)
                     /locus_tag="PK109_03320"
     CDS             complement(1401..>2438)
                     /locus_tag="PK109_03320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRH24591.1"
                     /translation="ANQIIEKNKNRKEKYSKTDNGPGERIRVHFAGTAEEEARFIASE
                     AGKRIRAGVPAEDIAVLYRTNFQSRAIEEAFLHERLPYRLLGTQFYDRKEVKDVIAWA
                     RLALDPEREADRMRAVQAPARGIGKVTLAKLAAGARDTLKPAERSKVEAFEKTLAALH
                     EAAKISPPSALIRLIIESSGMKTALQKGGEDDAERLLNAEEVATIASRHDDTPGLEGL
                     SAFLADIALVGDQDEMDRKKEKPGITLMTVHAAKGLEFDTVFVSGLEEGLFPHEGMGG
                     EEERDEEEERRLFYVAVTRAKNRLYLTLARVRRIYGTDYLQTASSFLSDIPAGLVEYA
                     HDGEDEDIIEA"
BASE COUNT          528 a          619 c          663 g          628 t
ORIGIN      
        1 ctgcttcgat agcggcagcg tagcttgaga cggaagtgcc gggaactgaa tggacggagg
       61 cgatttggta ggacgtagat acctgtgccg ctacccgggt ctttgcttcg ctcacgaggc
      121 ttgcaacaag cgcgctgttg tcgcccgtac cgcccttccc cgttgttaca taggtctcaa
      181 agaaggattt tgcgaactcg tctgttgccg agcctggggt aggtgcgggc acttcaaagt
      241 cgtcatcagt aaggggttca ggagccgtcg gggtctctga tttaaagcgg agcgctactt
      301 taccagctgc aaccgcctcg ctatcggtaa ggcctgcttc taccgagtgc gggtttgaag
      361 gatcggttcc atacagggac tcctgccagt ctgggaggtt atcaccatcg gtatcctttt
      421 ttgcgtactc ctttaatagc tcatcggtgg aggtagcacc tgcgatggtg agctggaagg
      481 gagaggaaaa atagaagccg gcgaacacaa agagcgccga aactaggagt gcggcaccaa
      541 tgcgaacggt ttttgaagaa agggccatag aagtcaagga aatagtacca cgcgtattca
      601 gcattccttc actctgtgtg cattatccac ccaataattg tgcgagtttt ttccacgtcg
      661 cgagcgacaa gtcttcagcg cgtgcttttt ctgaaataga cgcttcggca aacgccgtag
      721 tgaggagaga tgcgtcggca acggcctcca agttacgaat caggagctta cgtttgtgtg
      781 caaacccggc atgcaggagg tcaaagaaga acgtttcggt gttctttgaa gaaaacgggt
      841 tcttaatatt cgttatggtg agtaccgctg agtctacgtt cggtgctgga agaaacgcac
      901 cacgggggac ggtgaaagaa tacttcgggt ctccgtatac ctttacggca agcgagagga
      961 tgctttcctt tttgctgcgc gctatgcgtt cagctacttc cttctggacc aagagtgtca
     1021 tcgtgaacgg tgcgtgagta gcaccaagga atgagcgaat gatttctccc gtgatgtagt
     1081 acggaatgtt ggcgactaac gcatagggtt ctcctataga agcgggatca aatgcgcgga
     1141 tatcgccgta cacgagcgtg agtttctttt cttttatttc agtcgcgaac gtttcttcaa
     1201 gcgtagcgat aagggtgtga tctgcttcta cggcaacgac tctacgtgcg cgagcaagta
     1261 acggacgggt gagcatgccc gtgcctggcc ctatctcaag cacggtatcg tttgctgtga
     1321 ggagtgccgc gtcagcaata cgctctgcga tacgcgcatg catgagaaag ttttggccga
     1381 gtgatttttt tgcgcgcata ctaggcttct attatgtcct catcttcccc atcgtgtgcg
     1441 tactccacaa gccctgcagg aatatcagag aggaacgatg acgcggtctg taaataatcg
     1501 gtaccgtaaa tacgacggac acgagcgagc gtgagataga gacgattctt agcacgcgtc
     1561 acggctacat agaaaagacg gcgttcttcc tcttcatcgc gttcctcttc cccacccata
     1621 ccctcgtgcg ggaataatcc ttcttcaagt cccgagacaa atacggtatc gaactcaagt
     1681 ccttttgctg cgtgtacggt cataagagtg atacccggct tctctttctt ccggtccatc
     1741 tcatcctggt ctcccacgag tgcaatgtct gcgaggaatg ccgacaaccc ttcaagcccc
     1801 ggtgtgtcat cgtggcgcga cgctatcgta gcaacttcct cagcgtttaa gaggcgttct
     1861 gcatcatctt ccccgccctt ctgaagggcg gtcttcatac cactcgattc aatgatgagg
     1921 cgtatgagcg ctgagggagg cgatattttc gcggcttcgt gaagcgcggc aagcgttttc
     1981 tcaaacgctt ctacctttga acgctctgct ggttttaagg tatcgcgcgc tcctgcggca
     2041 agttttgcaa gtgttacttt cccaataccg cgggctggcg cttgcacggc gcgcatacga
     2101 tctgcttcgc gttcaggatc aagcgcaagg cgtgcccagg caatgacgtc tttcacttct
     2161 ttgcggtcat agaattgcgt accaaggaga cgatacggca gtcgttcatg gagaaacgct
     2221 tcttctatag cgcggctttg gaagttcgtg cgatacaaca ccgcaatgtc ttccgccggc
     2281 acgccggcac gaatacgttt tcctgcttcg cttgctatga agcgtgcttc ttcttcagca
     2341 gtgccggcaa agtgtacgcg gatacgttcg cccgggccgt tatcggtctt tgaatatttc
     2401 tctttacgat tcttgttctt ctcaatgatc tggtttgc
//
LOCUS       DAORIN010000088         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_12496_length_2399_cov_6.595990, whole genome shotgun sequence.
ACCESSION   DAORIN010000088 DAORIN010000000
VERSION     DAORIN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12496_length_2399_cov_6.595990"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PK109_03325"
     CDS             <1..733
                     /locus_tag="PK109_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRH24592.1"
                     /translation="HPETGSTGDGFGWLKALGHTVSDPTPTIVPLKTKEKWVRDFLGT
                     TLPSVKITFYLNGTKRFHKTGNILLAHFGLTGPTILNAAGEVADLLHEGTVTATIDLF
                     PKQDIGSLDRTLIALFDANKNKALRNALRELLPPGSADIVLTLTPSLSGEKKVHSITK
                     EERRALIDAMKGLPITIAGLMGLDRAVVADGGVPLSEVDMRTMRSLKCSNLFITGDLL
                     NITRPSGGYSLQLCWTTGYIAGRSA"
     gene            773..2260
                     /locus_tag="PK109_03330"
     CDS             773..2260
                     /locus_tag="PK109_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRH24593.1"
                     /translation="MAKSSEKGGGGGPQEAVLRFEDVSFGYGHNHPIIDEANFSLRRG
                     MKVTVMGQNGAGKSTIFSLITEERHPDSGKIQKTRGLTIALSRQVIAPAERELTVTEF
                     FQKQFAEKVYDIAPRIDEVLEVVNLHAPHDRIVKTFSGGQQARLLLASALIQDPDLLL
                     LDEPTNNLDTAGIEHLTTFLKNYQKTCMVISHDADFLNSFSDGVLYLDVHTQKVEQYF
                     GNYFDLLKEITARIERENRKNAQLAKKIQDNKDKANFFAQKGGKMRLVAKKMRTEIEE
                     MEEEKVDVRREDKTIRPFTIPVPADINGEILRIESLTIIDPKTHKPKKQSASIVLKKK
                     QHLLLKGPNGIGKSTLLESLANGSAEGAKISAGIKVGYYRQDFSTLNFEDTVFDSLLN
                     VMKKQEEERMRAVAAGFLITGNLMGKKIGMLSEGQKGLVAFARLVLEAPGLLILDEPT
                     NHINFRHLPVIAKALDSYEGAMVLVSHVPEFVAQIRIDETLELGK"
BASE COUNT          599 a          623 c          630 g          547 t
ORIGIN      
        1 acatccggag acggggtcta ctggggacgg gttcggatgg ctcaaagcac tcgggcacac
       61 ggtgagtgat cccaccccga ccattgtccc gttaaagacg aaagagaaat gggtacgaga
      121 ctttctcggc accacgctgc cttcagtaaa gattaccttt tacctaaacg gcaccaaacg
      181 ctttcacaaa accgggaaca tacttttggc gcactttggt ctcaccggcc ccactatcct
      241 taacgctgca ggtgaagtgg ccgatcttct ccatgaggga acggtaacag ccaccatcga
      301 tctcttccca aagcaagaca tcgggagtct ggatcgcacg ctgattgcgc tgtttgatgc
      361 gaataagaat aaggcgctcc gcaatgcgct tcgggaactt ctgccgccgg ggtccgctga
      421 catcgtgctc acccttacac cctccctttc aggagaaaag aaagtgcata gcatcactaa
      481 ggaagaacgc cgagcactta tcgatgcgat gaaaggactt ccgataacta ttgcgggact
      541 catgggactt gatagggcag tggtggccga tggtggcgtt ccgctttcag aagtagacat
      601 gcgcacgatg cgttccctta agtgttcaaa tcttttcatc accggtgacc ttcttaacat
      661 cacacgccct tcgggcggct actcattaca gctctgctgg accaccgggt acattgccgg
      721 acgttctgca taacactttc gcaacgtatc ctaatgcggt aaggtgggaa taatggctaa
      781 gagttcggaa aagggcggag gcggaggacc gcaagaagcg gtcctccgct ttgaggacgt
      841 atcctttgga tacggccata atcatcccat cattgatgag gctaacttct cgctgcgacg
      901 tggaatgaaa gtgaccgtca tgggtcagaa tggtgccggt aagtccacca tcttttcgct
      961 catcacggaa gagcgtcatc cagattcagg aaagatacag aagacccgag gcctcactat
     1021 cgcgctctcg cgtcaggtaa ttgcgcctgc ggagcgtgag ctcaccgtca ctgagttctt
     1081 ccaaaagcaa tttgccgaaa aggtgtatga catcgctcca cgcattgatg aggtacttga
     1141 agtagtgaat ctccacgctc ctcacgaccg tatagtgaaa acattctctg gcggtcagca
     1201 ggcacgtctt cttcttgctt ccgccctcat tcaggacccg gatctccttc tccttgatga
     1261 gccgacgaat aaccttgata ccgcaggcat tgagcacctc acgaccttcc tcaaaaacta
     1321 ccagaagact tgcatggtta tttcccacga tgctgacttc ttgaactcct tctctgacgg
     1381 cgtcttgtac cttgatgtgc atactcagaa agtagagcag tacttcggta actacttcga
     1441 tctcttgaaa gaaatcacgg cccgcatcga gcgtgagaac cgtaagaatg ctcagcttgc
     1501 gaagaagatt caggacaata aggacaaggc gaacttcttc gcgcagaagg gaggtaagat
     1561 gcgtctcgtt gctaagaaga tgcgcacaga gattgaagag atggaagagg agaaggtgga
     1621 tgtgcgccgg gaggataaga ccatacgtcc gtttactatt cccgttcccg ctgacattaa
     1681 tggagagata ctgcggatcg agtctctcac catcattgat ccgaagacgc ataagccgaa
     1741 gaagcaaagc gcgtctatcg tgcttaagaa aaagcagcac ctgctcttga agggtccgaa
     1801 cggtatcggt aagtcgaccc tgcttgagtc tcttgccaac ggcagtgcag aaggcgcgaa
     1861 gatatctgcc ggcatcaaag tcggttacta ccgtcaggac ttctccacgc ttaactttga
     1921 ggatacggtc tttgattcgt tgctgaacgt tatgaaaaag caggaagagg aacgcatgcg
     1981 cgccgttgct gcaggattcc tcatcactgg gaacctcatg ggaaagaaaa tcggtatgct
     2041 ctcagaaggg cagaaggggt tggtagcgtt tgcgcgcctc gtgcttgaag cgccgggact
     2101 ccttattctt gatgagccga ccaatcacat aaactttcgc caccttccgg tcatcgcaaa
     2161 agcgctcgat agctatgaag gagcgatggt gctggtgtcc cacgtccctg agttcgttgc
     2221 acaaattcgc atcgacgaaa cgttggagct cggtaagtaa aacaaaaagg cccgcgtgtg
     2281 cggcgggcct ttcggagctg tgcgcagtac ttactgctcc gggcgatggt tggcgaacca
     2341 atcttcgagc gctttgcgtc gttcacgctt gaaaggaatc tgttgaccct catggcgaa
//
LOCUS       DAORIN010000089         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_12754_length_2367_cov_6.209343, whole genome shotgun sequence.
ACCESSION   DAORIN010000089 DAORIN010000000
VERSION     DAORIN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12754_length_2367_cov_6.209343"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK109_03335"
     CDS             <1..227
                     /locus_tag="PK109_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24594.1"
                     /translation="LDEQPFPRFLFTAMRANACFSLPMFEVDASVMVSEMQHWQHLND
                     RLTLVRFTRINDKGQLVMFYGYILEKAWDV"
     gene            complement(284..832)
                     /locus_tag="PK109_03340"
     CDS             complement(284..832)
                     /locus_tag="PK109_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24595.1"
                     /translation="MNTNAIIGLIVAIVVVGGGAWFFSQHKAGTDLSGTGTTENTVGE
                     SGTLSLKELMMMGGSRKCEVTVTTAEAPATGTVYVSGTDVRSDIVTKPAAMNGATISA
                     HMIKSGDYMYSWSDMIPQGVKVKITAAESAGAQAGYDANSQVQYSCSPWIPDASKFEV
                     PSTVTFMEFSGGAGMPTGYPKP"
     gene            complement(863..1423)
                     /gene="cyaB"
                     /locus_tag="PK109_03345"
     CDS             complement(863..1423)
                     /gene="cyaB"
                     /locus_tag="PK109_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRH24596.1"
                     /translation="MHEVEVKAALSDKEGVMRALEERGCIFSAPLTENDTLYALQVGD
                     MESYNRNANFLRIRERSNGDIVFTLKHHPDRHEGRPDSMPLEHETTIGSKEEMEQALL
                     LMGYQEVVRTDKVRQKGRIGKWEVCVDDVEGLGSFIELEELTDGKETQRVIDEMLAFL
                     AELGIARGDMFADRYDIALLKKRWGV"
     gene            complement(1426..1662)
                     /locus_tag="PK109_03350"
     CDS             complement(1426..1662)
                     /locus_tag="PK109_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24597.1"
                     /translation="MDTKLEEIAATLTAIRAQGTDWKRAMYMGLFQGAGVIIGSIIAL
                     ALLGWVLTLLGVVPGFDGFETYLNSIVTEYQRTR"
     gene            complement(1667..2260)
                     /locus_tag="PK109_03355"
     CDS             complement(1667..2260)
                     /locus_tag="PK109_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRH24598.1"
                     /translation="MIKAVLFDLDGIVIVGREKYFSHKLAEEKGIPLEKVDAFFLNDF
                     KSCVMGTCDLKEGISPYLPEWGWRGTVEEFLENWFSSERTRDENVIAIIDRLRAAGVK
                     CYIASRQEKYRMRYLLEEVGLALHFDGTFCTSDIGYDKSASDYWDRVIAALGLQPEEI
                     LFFDDKQVNVDRARELGIDAHFYDSISVLQEQTASLY"
BASE COUNT          574 a          666 c          523 g          604 t
ORIGIN      
        1 cgctcgacga acaaccgttt ccgcgatttc tgttcacggc gatgcgcgcc aacgcttgct
       61 tcagccttcc aatgtttgaa gttgatgcgt cggtaatggt gtcagagatg caacactggc
      121 aacacctcaa cgacaggctc acgttggtgc ggttcacccg cataaacgac aagggtcagc
      181 tcgtcatgtt ctacggctat attcttgaga aggcctggga cgtgtaacga attcgggcgg
      241 aagcaatgac gcttccgccc gaatctctta tatatctacg aacttacggc tttgggtacc
      301 ctgtcggcat tcccgcccca cctgagaact ccataaacgt taccgtcgac ggtacctcaa
      361 acttacttgc gtcaggaatc catggactgc acgaatactg cacttgagag tttgcatcat
      421 atccggcctg agcacccgcg ctttctgctg cagttatttt caccttcact ccctggggaa
      481 tcatgtcgct ccatgagtac atgtagtctc ctgacttgat catgtgcgct gaaatggtgg
      541 caccattcat cgctgctggc ttcgtgacga tgtcggagcg tacgtcagta ccagaaacat
      601 agacggtacc cgtagctggg gcctcagcgg tggtcaccgt cacttcgcac ttacgtgagc
      661 cgcccatcat catgagttcc tttaacgaaa gcgttcccga ttctcctacg gtgttttcag
      721 tagtaccggt gcctgaaagg tccgtcccgg ccttatgctg cgagaagaac cacgcaccac
      781 cgcctacaac gacgatagcg acaatgagtc cgataatggc attagtgttc atatacgttt
      841 gttacttgta aatctgagcg gattatacac cccaccgttt cttgagaaga gcgatgtcgt
      901 agcggtccgc aaacatatca ccacgtgcta tgccaagctc tgcaagaaac gcgagcatct
      961 catcgataac gcgctgcgtc tccttcccat cggtaagctc ttcaagttct ataaaagaac
     1021 caagtccctc gacatcatcg acgcatactt cccactttcc tattcgtccc ttctggcgca
     1081 ccttatcggt gcgtactact tcctgatatc ccatcaacaa gagcgcttgc tccatctctt
     1141 ctttggaccc aatggtcgtc tcatgctcaa gcggcatgga gtcaggacgt ccttcatgac
     1201 ggtctgggtg atgctttaag gtaaacacaa tgtcaccatt gctgcgctca cgtatgcgaa
     1261 ggaagttcgc atttcggtta tatgactcca tatccccgac ttgaagcgca tagagcgtgt
     1321 cgttctctgt aaggggtgct gaaaagatac agccccgctc ttcaagcgca cgcatcaccc
     1381 cttctttatc gctaagcgcc gcctttactt caacttcgtg catacctagc gagtgcgctg
     1441 atactccgtc acgattgaat tcaaatacgt ttcaaagcca tcaaatcctg gaaccactcc
     1501 gaggagcgta agcacccagc cgagaagcgc aagcgcaata atactgccga tgatgacgcc
     1561 cgcaccttga aagagcccca tgtacattgc ccgcttccag tcagtacctt gtgcgcgtat
     1621 tgcggtaagc gttgcggcga tttcttcgag tttcgtatcc atatcgctag tataaagagg
     1681 cagtctgttc ctgcaataca gaaatgctgt catagaagtg cgcatcgata cccagttcac
     1741 gcgctctatc gacattcacc tgcttgtcat caaagaacag aatctcttct ggttgcaatc
     1801 cgagtgcggc aataactcgg tcccaatagt cagatgcgct tttgtcgtac ccaatgtctg
     1861 aggtgcagaa cgtaccatca aaatgtaatg ccaaccccac ttcctcaagt aaatagcgca
     1921 tgcgatactt ttcctgacgc gatgcgatgt agcacttcac accagcagcg cgaaggcgat
     1981 caataatggc aatgacgttt tcgtctctcg tgcgctcgct cgaaaaccag ttctcgagaa
     2041 actcctccac ggttccgcgc catccccatt cgggcaggta cggggaaatt ccctccttaa
     2101 gatcacaggt tcccatgacg cagcttttaa agtcgtttaa gaagaacgca tctacttttt
     2161 ccaagggaat acctttttct tcagcaagct tatggctgaa gtatttctct cttccaacga
     2221 tgacgatgcc atcaagatca aacaagactg ctttaatcat aagaaccagt agttaatgac
     2281 cccaacaatc acaataacgg tgtatacgag tgtcgtgaga aatatgccta tctgccgcgc
     2341 ttctttccat gccttgtatg agctata
//
LOCUS       DAORIN010000090         2343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_13005_length_2343_cov_5.436189, whole genome shotgun sequence.
ACCESSION   DAORIN010000090 DAORIN010000000
VERSION     DAORIN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13005_length_2343_cov_5.436189"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PK109_03360"
     CDS             complement(<1..995)
                     /locus_tag="PK109_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRH24599.1"
                     /translation="MRTDKALPQKTFAAFRAPRVPFPDLVAHQRDSYTWLVKEGLNEL
                     FKEFSPIPDYSGKKFELRFDGFELGEPKGDEEMARENMRTYEVPLKASVTLLNKTMGT
                     EKSQDIFLADLPMMTPHGSFVVSGVERAIVPQLARSFGAFFIAEDIKGKRFFGAKIIP
                     SRGVWIEIDSEADGAVFVKIDRKRKFPITNLLTIFGAGSGEEIAKHFKGNAVALAALK
                     ATLAHDTTKTPEEAYVEIHRRMRDGDLATAENAKSYVNLIFTPERYDLSKVGRNRLNA
                     RFGLPTDAKALNQRALTLDDLVRIVGEIARLNEDPNAVPDDIDHLGFRRVRFVGEL"
     gene            complement(1534..2184)
                     /locus_tag="PK109_03365"
     CDS             complement(1534..2184)
                     /locus_tag="PK109_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRH24600.1"
                     /translation="MVRFHLITLFPEACDAYLSASILGRARKNKKIDVKYQSPRDFVT
                     NKWGKVDERPYGGGPGMVMTALPVVKAAKKSLARAKGKTAIIWFQPGAKAFDNAEADK
                     LTKFNDVVLVCGRYEGIDERAKKMLKTMGTLKTYSVGEAIYTGGEVPSLAIVDAVTRR
                     LPGVLGKDESVEERRVASREVYTRPDTVEFGGKKYAVPKVLKTGNHAKIEDWKKGE"
BASE COUNT          516 a          621 c          621 g          585 t
ORIGIN      
        1 agttctccaa cgaagcggac gcggcggaag ccgaggtgat cgatatcgtc tggaaccgcg
       61 tttgggtctt cgttcaagcg agcgatttca ccaacgatgc gaacaaggtc atcaagcgtg
      121 agtgcgcgct ggttaagcgc cttagcatcc gttggaagac cgaagcgagc gttcaagcgg
      181 ttgcgtccta ccttagaaag gtcgtagcgc tctggagtga agatgaggtt cacgtaggac
      241 tttgcgtttt ccgcagtcgc aaggtcacca tcgcgcatgc ggcgatggat ttctacgtat
      301 gcctcttctg gggtcttcgt ggtgtcgtgc gcgagtgtcg ctttaagggc agccaaagca
      361 acggcattac ccttgaaatg cttagcaatc tcttcgcctg aaccggcacc gaagatggtg
      421 aggaggttcg tgattggaaa cttacgcttg cggtcaatct ttacgaagac ggcgccgtca
      481 gcttccgaat caatctcaat ccatacaccg cgagaaggaa tgatcttcgc accgaagaag
      541 cgctttcctt ttatatcttc tgcgatgaag aacgcaccga aggaacgagc gagctggggg
      601 acgatagcgc gctcgacgcc ggatacgacg aaggacccgt gcggggtcat cattgggagg
      661 tcagcaagga agatgtcctg gctcttctcc gttcccatcg tcttgttgag gagggtgacg
      721 gatgccttca acggcacttc gtaggtacgc atgttttcgc gtgccatctc ttcgtcgcct
      781 ttgggctcac cgagctcaaa gccgtcgaag cgaagttcga acttctttcc tgagtagtca
      841 ggaattgggg agaattcttt aaagagttcg ttcaaccctt ccttaacgag ccaggtgtag
      901 gaatcgcgct ggtgcgcaac gaggtcaggg aacggaacgc gaggggcacg gaatgcggcg
      961 aacgtcttct gcggaagtgc tttgtcagtg cgcatgttcg tctaatagtt aaataatagt
     1021 gaagttctga aatgcagaaa tactcgtaca cgacgagccg cgtctccgga tgctctccga
     1081 gacgctttcc ttataaatct tctattgtcg cccccagagt gcccccgtca ctcactcagt
     1141 tctggttagc cacagaatac tccccttcat gaagcgggtc aagggggtgt ggggatagga
     1201 agttgccgta aatattgaca atatagatag ttactttata agtatctata aggacctaca
     1261 ggactatcga cgaggatgtc ctcgcgcctt gtgggcatag ttgagatgaa agatcttgga
     1321 tcacaaatgg cagacgctgc catataccaa atggatcgcg aagccgagcg tcttcaaaaa
     1381 gcacgcgagg aaggcgacag aaaatggtgg atcggcttag ccactggaat catcgtcgga
     1441 gtcttgggca cacttttagt gctcaggctg acgggaatga tctagttagg ctgagaccag
     1501 ggctggcgtg tcgtatgacc gccagccctt gttctattct cccttcttcc agtcttcaat
     1561 ctttgcgtga ttccctgttt tcagaacttt tggtactgcg tacttctttc ctccaaattc
     1621 aacagtgtca ggacgcgtgt agacctctct cgacgcgaca cgtcgctctt ctaccgactc
     1681 atccttacca agcactcccg gaaggcgccg cgtgacggca tcaactatgg cgagcgacgg
     1741 gacttccccg cccgtgtata ttgcttcacc tacggaatag gttttaagcg tacccatggt
     1801 cttgagcatc tttttcgcgc gctcatctat tccttcatag cggccacaga ctaagacaac
     1861 atcattgaac ttcgtgagct tatccgcctc cgcgttatca aacgcctttg ctcccggctg
     1921 gaaccatatg atggcggtct ttccttttgc tcgtgcgagc gactttttcg ctgccttcac
     1981 cacgggaagc gcggtcatga ccatgccggg tccacctcca tacggacgct catctacttt
     2041 tccccatttg ttcgttacaa agtcccgcgg actttggtac ttcacgtcga tcttcttatt
     2101 ctttcgggcg cgcccgagta tggacgccga aagatacgcg tcgcacgcct ctgggaagag
     2161 cgtaatgaga tggaagcgca ccatacccat tatgggagga tatccgtctt gattttgaag
     2221 tcgccgcgga atccggcaga tccttcaata gactcttcga gcgttcctac cattatctgg
     2281 tagccaccgt cttcgtgtgc gaagacgccg atctcgtcac cacctcccgc gtaccagcat
     2341 tgt
//
LOCUS       DAORIN010000091         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_13689_length_2278_cov_4.579847, whole genome shotgun sequence.
ACCESSION   DAORIN010000091 DAORIN010000000
VERSION     DAORIN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13689_length_2278_cov_4.579847"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PK109_03370"
     CDS             <1..1025
                     /locus_tag="PK109_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24601.1"
                     /translation="LAGQDGETRVFRFTAGALKDADSREFGVSYTENDASVTIAKPFF
                     AVGLEVNRESAPTIVAKAGESISGLLSWTNALPTPIQDGAITVQLSGDALNASQVNAT
                     NGFYQSSTRTVRFDRDTSSGLALLGPGDTGNGSFTFTTKTGSALNALRNPVITLAVSV
                     SGRRSGQNGATETVSSTIVRTVKIQSDLSFSMNALRTTGPFTNTGPWPPEADKESTYT
                     IRLTAGNTVNTVANTEAKMTLPSYVRYTGQTSGGTVKYNEATREVVWSLGDVGPGAAR
                     EASFQVALLPSVSQKGTSPTLVSDAVLTGFDRFVQKQLSVTAFSVNTQTKTDPNYQYD
                     AGVVKQ"
     gene            1022..>2278
                     /locus_tag="PK109_03375"
     CDS             1022..>2278
                     /locus_tag="PK109_03375"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRH24602.1"
                     /translation="MKNETPKLEDIVSLAKRRGFVYPGSEIYGGLAGTFDYGPNGVEL
                     LNNLKRAWWKANVYDRESFYGLDSAMFKHPRTWEASGHTKGFSDPLAECRECNTRIRV
                     DKELGAIGVTADEKMSIEEINALFDAHRETIKCPKCGKKNFSEVRAFNLLVKSNLGDF
                     TSDGSEPVYLPGEACQGIYLDYKNIVDTMSPKVPFGVAQIGKAFRNEISPRNFLFRTR
                     ELEQADTQLFVRPNENQEAYQEAKKARFDWYLSLGIREEKLRWHQHENLVFYAKDAWD
                     IEYEFPSLGFDELEGVHDRTDYDLTQHQEHSGVDLRYTDSETGEKFIPWIIETSVGMG
                     RMFMAFLCDAYRVDELGGEPRVYLALHPSLAPYRAAVFPLLKNKPELVSKARGVYLAL
                     KKAGIRVAFDDNGNVGKRYRRQDEIGT"
BASE COUNT          553 a          574 c          639 g          512 t
ORIGIN      
        1 cgttagccgg ccaagacggt gagacccgtg tcttccgctt tactgcaggc gctctgaaag
       61 acgctgactc ccgtgagttt ggggtgtcgt acacagaaaa cgatgcgagc gttaccatcg
      121 ctaaaccatt ctttgccgtt gggcttgaag tgaatcgcga gtcggcgccg accattgttg
      181 caaaagcagg ggagtcgata agcgggcttc tctcgtggac caacgcactg cctaccccga
      241 tccaggatgg cgcgattacg gtgcagctct caggggatgc gttgaacgct tctcaagtga
      301 atgcgaccaa cggtttctat cagtcctcaa cacgcacggt gcgctttgac cgcgacacct
      361 cgtcagggct cgcgcttctt gggccaggag acaccggaaa cggtagcttt acctttacga
      421 caaagacggg aagtgcgttg aatgcattac gtaacccagt catcacgctt gcggtatcgg
      481 tctcagggcg tcgctccggc cagaacggcg cgacggaaac cgtctcttct accatcgttc
      541 gcaccgtcaa gattcaaagc gatctttcgt tctctatgaa tgcgcttcgt acgacgggac
      601 cgtttacgaa cacggggcca tggccgcctg aagccgacaa agagagcacc tataccatcc
      661 gccttacggc agggaatacg gtgaatacgg ttgctaatac tgaagctaag atgacgctcc
      721 cgtcctatgt ccgctatacg ggccaaacaa gcggcggcac ggtgaagtac aatgaggcca
      781 cgcgtgaagt ggtatggtcc ttgggagacg ttggcccggg agcagcgcgt gaagcgtcgt
      841 ttcaggtagc gctcttgcca agcgtttccc aaaaaggtac aagtccaaca ctcgtctctg
      901 atgcggtact gactggattt gaccgattcg tgcagaagca gctttccgtg acggccttct
      961 ccgtgaatac gcagacgaag acggatccaa actatcagta cgacgcgggc gtggtgaagc
     1021 aatgaaaaac gaaactccaa aactcgaaga tatcgtctca ctcgctaagc gacgcgggtt
     1081 tgtgtacccc ggttcagaaa tttatggcgg tctcgcgggc acctttgatt acggaccaaa
     1141 cggcgttgag ctgttgaata acttgaagcg tgcgtggtgg aaagcgaacg tgtatgaccg
     1201 cgaaagtttt tatggtctcg attcagcaat gtttaagcat ccgcgtacgt gggaagcgag
     1261 cggtcacacg aaaggattct ctgatccttt ggcagaatgc cgcgaatgca acacgcgtat
     1321 ccgcgttgat aaggagcttg gtgccatagg ggtcactgct gacgagaaga tgtcgataga
     1381 agagattaat gcactgtttg atgcccatcg cgaaacgata aagtgtccga agtgcgggaa
     1441 gaaaaacttc tcagaagtac gtgcatttaa tttgctcgta aagtcgaacc ttggtgactt
     1501 cacgtctgac ggcagtgaac ccgtgtatct tccgggcgaa gcatgtcagg gtatttatct
     1561 tgattacaaa aacatcgtcg acacgatgag cccaaaggtt ccgtttggcg tcgcacagat
     1621 agggaaggcg tttcgtaacg agatatctcc acgaaacttc ctgttccgta cgcgtgagct
     1681 tgagcaggcg gatacgcagc tctttgttcg tccaaacgaa aaccaagaag cctaccaaga
     1741 agcaaagaag gcgcggttcg actggtacct ctcactcggt atacgcgaag agaaactccg
     1801 ctggcatcag catgagaacc tggtgttcta tgccaaggac gcatgggata tcgagtacga
     1861 attcccatcg cttgggtttg atgagcttga gggtgtgcat gatcgcaccg actatgacct
     1921 cacgcagcat caagagcact cgggtgtgga cttgcgctat accgactctg aaaccggaga
     1981 gaaattcatt ccgtggatta ttgaaacctc agtaggtatg ggtcgcatgt tcatggcctt
     2041 cctatgtgat gcgtaccgtg tcgatgaact gggtggcgaa ccgcgcgtgt accttgcgct
     2101 ccacccaagt cttgcgccgt accgcgcggc agtattcccg cttcttaaaa acaaacctga
     2161 attagtttct aaagcgcgtg gagtatatct tgcgcttaag aaggcgggca ttcgcgttgc
     2221 gtttgatgac aacggtaacg tggggaagcg gtaccgccgt caggatgaga taggtacc
//
LOCUS       DAORIN010000092         2266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_13809_length_2266_cov_5.858435, whole genome shotgun sequence.
ACCESSION   DAORIN010000092 DAORIN010000000
VERSION     DAORIN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13809_length_2266_cov_5.858435"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PK109_03380"
     CDS             <1..549
                     /locus_tag="PK109_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRH24603.1"
                     /translation="IEIENENSGFTPSPAWKEKTYGEPWRIGDTYNTSIGQYAMQITP
                     LEAVRAVAAVANGGKLLRPTLVKDAPIQGESIAISPESLKIAREGMRLGVTEGTSIGL
                     NPLSYVKAAGKTGTAQLGVHNEYYNSWAVGFFPYDKPKYAYVVVMERGPAGNPTGGIY
                     VMSQVFSKLRYSAPEYFGLSAE"
     gene            564..1724
                     /locus_tag="PK109_03385"
     CDS             564..1724
                     /locus_tag="PK109_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24604.1"
                     /translation="MSEKGEGQLEKKGPTRRGVLSGMGNVAKGIWAFNAVAWTAIGAQ
                     EIYKYRGEILDVLLDAQHAGNENVQSARRFLKELYGVEVLFGKDHQNESYEAAGLTAY
                     EAQRGLSAIISALMKYPHDFFKENPLTPVIRIGKGVALHHENETHRVGGFASQEEGYF
                     MIEYQERLSTMVEIFHHEFYHILDARHASEKRDAEWKAINAAAGYTYSGDVFEDPAPY
                     RSEFLDLFSSAHGAANPQEDRAEIAERLMTIYGYWGLTERLEDMEDGPIKEALERKIA
                     LVKEDFRVWSNGTMDEAFWLRIERESLRSAEDFTYEPLGSYTFYPPPSLDTGLVLGEP
                     SRVYDTEPIVITGSRDSYPPSIAPELIISDQQFIERETRDWYGDGRELRGRR"
     gene            1781..2251
                     /gene="greA"
                     /locus_tag="PK109_03390"
     CDS             1781..2251
                     /gene="greA"
                     /locus_tag="PK109_03390"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRH24605.1"
                     /translation="MTDQQDTVLSQEKFDELAKELEQLKTVRRAEIAKNLEYARSLGD
                     LSENAEYQEARELQAATEERIRKLEELVKRAQIMTDGKKKDIVGFGSAVTIKKEGGEA
                     HEYKIVGSEEADMREHKLSHVSPLGAALMGKKKGDVFTFETPNGKQTYTIQKVA"
BASE COUNT          622 a          480 c          648 g          516 t
ORIGIN      
        1 attgaaattg agaacgagaa ctcaggattc acaccctcgc ctgcgtggaa agaaaagacg
       61 tatggggagc cgtggcgcat cggagacacg tataacacct ccattggtca gtacgcgatg
      121 cagataacgc cgcttgaggc ggtacgagcc gtggcggcgg ttgcgaacgg cgggaagctg
      181 cttcgtccga ccttggtgaa agatgctcct atacagggag agtcaattgc cataagtccg
      241 gagagtctta agatcgcgcg cgaaggtatg cgtcttggcg tcactgaagg tacctcgata
      301 ggattgaatc cactttcgta cgttaaagcg gcaggaaaga ccggtaccgc gcagcttgga
      361 gtgcataacg agtactacaa ctcatgggcg gtgggattct tcccgtacga caaaccgaaa
      421 tacgcgtatg tggtggtgat ggagcgtgga cctgccggga acccaacggg cggtatctat
      481 gtgatgagcc aggtgttttc taagcttcgc tactcagcgc ctgagtactt cggccttagt
      541 gctgagtaag atactatttt cctatgagcg aaaaaggaga agggcagcta gagaaaaaag
      601 ggccgacccg tcgaggagtg ctatcgggaa tgggtaacgt agccaaaggg atatgggcct
      661 ttaatgcggt agcgtggacc gctatagggg cacaagagat atataaatac cgtggtgaaa
      721 ttcttgatgt tcttcttgat gcgcagcatg cgggaaatga aaatgtgcag tcggcacgaa
      781 ggtttttgaa agaactctac ggcgtagagg tgcttttcgg caaagatcat caaaatgaaa
      841 gttatgaagc ggcaggactt acagcgtatg aagcacagcg tggtctttca gctatcataa
      901 gcgcgttgat gaagtatccc catgattttt ttaaggaaaa tccacttacg cctgtcatac
      961 ggatagggaa aggggtagcg ttgcatcatg aaaatgaaac gcatcgtgta ggaggatttg
     1021 cgagtcagga ggaggggtac ttcatgattg aatatcaaga acgtctttca acgatggttg
     1081 aaatattcca ccacgagttc tatcacatcc tggatgcacg ccatgcatct gagaagcgtg
     1141 acgcagaatg gaaggcgata aacgctgcgg caggatacac gtattcggga gatgtatttg
     1201 aagatccggc gccctataga agtgagttcc tcgatttatt ctcttcggct catggagcgg
     1261 ctaatccgca agaagatcgg gcagaaattg cagagcgact catgaccatc tatggatatt
     1321 ggggattaac cgaacgtctt gaagatatgg aggatggtcc gattaaagag gcgctggagc
     1381 gtaagatagc gctcgtgaaa gaagattttc gcgtatggag taacggaacg atggatgaag
     1441 cgttctggtt gcgaattgaa cgtgaatcat tgcgctctgc tgaggacttt acgtatgagc
     1501 cgttaggctc ctataccttc tatcctcctc cttctcttga tacgggactc gtacttggtg
     1561 aaccatcacg agtgtacgac acggaaccaa ttgttataac gggatcgcgt gattcgtatc
     1621 cgccctctat tgctcctgaa ttaataattt ctgatcagca atttattgag cgagagacca
     1681 gagactggta cggcgatggt cgtgagctcc gtgggcgtcg ctagtattaa ttgacgccct
     1741 gccaagtacc cgtacaatag ggacacctaa ccatattttc atgactgacc agcaggacac
     1801 agtgctctct caagagaagt ttgatgagct ggccaaagag ctcgagcagc tcaagaccgt
     1861 gcgtcgtgca gaaattgcaa agaaccttga atacgcacgt tcgctcggtg acctttctga
     1921 gaacgctgag tatcaggaag cacgcgaact ccaggctgca accgaagagc gtattcgtaa
     1981 gctcgaggaa ctcgtgaagc gtgcgcagat catgactgat ggtaagaaga aggacatcgt
     2041 cggcttcggt tccgcagtga ccattaagaa agagggaggt gaagcgcacg agtataagat
     2101 tgttgggagc gaagaggcag atatgcgcga gcacaagctc tctcatgttt ctccacttgg
     2161 tgcggcactc atgggaaaga agaagggaga cgtgtttacg tttgaaacgc caaacggcaa
     2221 gcagacctac acgattcaga aggtagcgta acgagagtac ataaaa
//
LOCUS       DAORIN010000093         2246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_14024_length_2246_cov_4.372889, whole genome shotgun sequence.
ACCESSION   DAORIN010000093 DAORIN010000000
VERSION     DAORIN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14024_length_2246_cov_4.372889"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /gene="fusA"
                     /locus_tag="PK109_03395"
     CDS             <1..362
                     /gene="fusA"
                     /locus_tag="PK109_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201871.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRH24606.1"
                     /translation="DVDSSEIAFKIAASMAFQEAANRAKPVILEPIMNVEVVVPDQFM
                     GDVTGSLSGKRGAIEGMEERGMNKAVHAKVPLSEMFGFTTTLRSMTEGRGSMTMEFDH
                     YEVVPTNVANDIIAARK"
     gene            complement(430..699)
                     /locus_tag="PK109_03400"
     CDS             complement(430..699)
                     /locus_tag="PK109_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24607.1"
                     /translation="MMTNPVSPSVPPSVVAVLFSSYTSAVLWLVLTTPFVLGALYNDP
                     RYWWFVALLVCFSLVTLLRTWLRIRAEKEREQEEKDRWLAQELEQ"
     gene            complement(828..1154)
                     /locus_tag="PK109_03405"
     CDS             complement(828..1154)
                     /locus_tag="PK109_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24608.1"
                     /translation="MTGTAPEALSTIEKPNWFLALVYGVSVLFYAGSLVVVTTIIILS
                     WRITFDLLLIVVGALIPITLFVALLPRGIGEIGLGILIASVICNILRKLFPKEDETNQ
                     EAYVIE"
     gene            1432..>2246
                     /locus_tag="PK109_03410"
     CDS             1432..>2246
                     /locus_tag="PK109_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRH24609.1"
                     /translation="MADAFDRSKPHMNVGTIGHVDHGKTTLTAAILNVLNLNAGAGYK
                     ARAENVENIDNAPEEKARGITIALHHSEYESPKRHYAHIDAPGHADYIKNMITGAAQM
                     DGAVLVVAATDGAMPQTREHILLAKQVGVPKLIVFLNKVDMVDDKDMVDLVEEEIREL
                     LTKQGFDKETPMIRGSGLKALEAKDANDEWALKVKELVDTMDSYFPDPVRDTAKPFLM
                     PIEDIFSIEGRGTVVTGRIERGIVKVGDEVEIVGIQPTTKTTITGIEMFNKQL"
BASE COUNT          567 a          590 c          602 g          487 t
ORIGIN      
        1 acgatgtgga ctcttctgaa atcgccttta agattgccgc atcaatggca ttccaggagg
       61 ccgctaaccg tgcaaagccg gttatcctcg agccaatcat gaacgttgag gtcgtcgtcc
      121 ctgaccagtt catgggtgac gtcaccggct ctctctctgg aaagcgtggt gctatcgaag
      181 gtatggaaga gcgcgggatg aacaaggcgg tccacgccaa ggtgccgctc tctgaaatgt
      241 tcggcttcac cacaacgctc cgctctatga ctgagggtcg tggctccatg accatggaat
      301 tcgatcacta cgaagtcgta ccgacgaacg tcgcaaacga catcatcgct gcacgaaaat
      361 aattaattga gaatgtgaaa agaaggacgc cccagctcgg ctggggcgtt tttcgtggag
      421 cgtaagcgac tactgttcga gttcttgtgc taaccagcgg tctttttctt cttgctcgcg
      481 ttctttttct gcccggatac gcaaccaggt gcgcaggagc gtgacgagag agaaacagac
      541 caagagtgct acgaaccacc aatatcgtgg gtcgttgtac agggcaccca gcacgaacgg
      601 ggtggtgagt acgagccaca gaacggcgga agtgtaggaa gaaaagagta ccgctaccac
      661 tgaaggaggg acggagggag aaacgggatt ggtcatcatc gcgtgtcttc tttcttcttt
      721 tgcaaagcgg ataggtgctt ctgacgggta ctatacgact tagctatggg aatgcaaaaa
      781 caaacgcccc ggggtaagcc agggcgttta tatgtcgggc gcgtagtcta ttcaatgacg
      841 tacgcttcct gattcgtttc atcctctttt gggaagagtt tccgaaggat attgcagatc
      901 actgatgcaa tcagaatgcc gagcccgatt tctccaatcc ctctcggaag aagggcaacg
      961 aagagcgtaa tcggtatcaa ggctccaacc acaatcagca gcaaatcaaa ggtaatccgc
     1021 caagaaagga taatgatcgt cgtaacgaca accaggcttc cggcatagaa caggacgctg
     1081 actccataca ccagcgcaag aaaccagttc ggtttctcga tggtcgataa ggcttccggt
     1141 gcagttccgg tcattagtgc ctccgtagta caggttaggg gccgtgaccc catacattca
     1201 ccctagcact cggcactcct tccgcaacta ggagttgacc tttctgaggg tgcgcgatac
     1261 cttatgaata acgcggattg gcgctctttt tattgacccc acacggccat tggctggcag
     1321 gggatggtaa aaggcgcttc gcgggtgggc taaaccagca aagcgccttt tatcgtttcg
     1381 gcgattaagg agcattcgct tatcaatcaa ttaccgttaa tttacgaaca tatggcagac
     1441 gcattcgatc gttcgaagcc tcacatgaac gtcggtacca tcggtcacgt tgaccatggt
     1501 aagaccacgc tcactgcggc aatccttaac gtgctcaacc tcaacgccgg tgccggctac
     1561 aaggcccgcg ctgagaacgt tgagaacatc gacaacgcac cagaagaaaa agctcgtggt
     1621 attaccatcg cgcttcacca ctctgagtac gagtcaccaa agcgtcacta cgctcacatc
     1681 gacgctccgg gccacgctga ctacatcaag aacatgatta cgggagctgc ccagatggac
     1741 ggcgccgtac tcgtggttgc ggcaactgac ggcgccatgc ctcagacccg tgagcacatt
     1801 cttcttgcaa agcaggtcgg tgtgcctaag ctcatcgtct tcttgaacaa ggtggacatg
     1861 gtagatgaca aggacatggt ggacctcgtt gaagaggaaa tccgcgaact tctcaccaag
     1921 cagggctttg ataaggagac cccaatgatc cgcggctcag gccttaaggc acttgaagct
     1981 aaggacgcga acgatgagtg ggccctcaag gtcaaggaac tcgtcgatac gatggactcc
     2041 tacttcccag atcctgttcg cgacactgca aagccattcc ttatgcctat cgaggacatc
     2101 ttctcgattg aaggccgcgg aacggtggtt acgggccgca tcgagcgcgg tatcgtgaag
     2161 gtcggtgacg aagttgagat cgtcggtatt cagccaacga ctaagacgac catcaccggt
     2221 atcgagatgt tcaacaagca gctcca
//
LOCUS       DAORIN010000094         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_14520_length_2205_cov_5.461860, whole genome shotgun sequence.
ACCESSION   DAORIN010000094 DAORIN010000000
VERSION     DAORIN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14520_length_2205_cov_5.461860"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2205)
                     /locus_tag="PK109_03415"
     CDS             complement(<1..>2205)
                     /locus_tag="PK109_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRH24610.1"
                     /translation="FLQGTVTKADATLMAGELHNPLIERARITAFKDWNNGRVALIVP
                     KVKLPRPEPSRAVNLEIPDAELARIGKEGIEDKDGTRRGPLALSLEELHAIRDYFRSK
                     KRAPTDVELESLAQTWSEHCKHTIFNDPLDEIEEGIYKRYIRGATEKVRAKKGMKDFC
                     VSVFKDNSGAISFNDDYLVTHKMETHNTPSALDPYGGAITGIVGVNRDALGFGLGAKP
                     IANLYGFCVGEPSDTRPLYRDAAKTRPMLSPQRILEGVVKGIEAGGNQSGIPTPLGFV
                     YADESYRGKPLVFAGTIGLIPRKSGGRLLHKKKARPGDYIVMAGGRVGADGIHGATFS
                     SEALSADSPATAVQIGDAITQKKFSDAIVRELRDKNLYTSVTDNGAGGLSCSVAEMAE
                     ECGGAVVDLSTVPLKYPGLTPWQIWISESQERMTFSVPKDKWKEMKAILDKHGTEATV
                     IGRFTNSGTCVVNYGGKKVMDIDLEFLHGGRPEKQLVTKKPTVAVRPLPARLPKIDKT
                     LLSLLARPTIGSYDFISNQYDHEVQGLSVTKPLVGRGRVNADAAVIRPLATDTKGVVL
                     SHGYAPSMSELDAYAMAGASIDIAIRNALAAGAPLDSLAILDNFCWSTSGAPERLYLL
                     KEAARACYDYAVTYGTPYISGKDSMYNDFRGFDEKGNAVHVAVLPTLLISAMGVMDDY
                     KKAMTFDFKTAGDRIYLIGKTRAEFGGSEFAKVAAGTFMSGRVPVTDAKENAS"
BASE COUNT          515 a          604 c          556 g          530 t
ORIGIN      
        1 gaggcattct ctttcgcatc ggttaccggc actcgtcctg acataaaggt gccggcagca
       61 acttttgcaa actcactgcc accaaactca gcgcgcgtct ttccaatgag atagatgcgg
      121 tcaccggcgg ttttaaaatc aaacgtcatg gctttcttat agtcgtccat gacgcccatg
      181 gcggagatga gaagcgttgg gaggactgca acatgcaccg cgtttccctt ttcatcaaat
      241 ccacggaaat cgttatacat actgtctttg ccggagatgt acggcgtgcc ataggtaacg
      301 gcatagtcgt aacaggcgcg tgctgcttcc ttaagaagat aaaggcgttc cggtgcccca
      361 gaggtggacc agcagaagtt atcaaggata gctaatgagt cgagcggagc gcccgcagca
      421 agtgcgttac gaatcgctat atcaatagac gctcctgcca tcgcgtatgc atcaagctcg
      481 ctcatcgatg gtgcatagcc atgagaaagc acgacaccct tcgtgtcggt ggccaatgga
      541 cgaatgacgg cagcgtctgc attcacccgt ccgcgaccca cgagtggctt ggtgactgag
      601 agtccttgca cctcatggtc gtactggttt gaaataaagt cgtagcttcc gatggtcgga
      661 cgtgcaagaa gagaaaggag ggtcttatct atcttaggaa ggcgtgccgg aagcggacgc
      721 accgcaactg tgggcttctt ggtaactaat tgcttttctg gacgtccgcc atgtaggaac
      781 tcaaggtcaa tatccattac ctttttcccg ccatagttca cgacgcaggt gccggagttc
      841 gtgaagcgac caatgaccgt agcttccgtc ccgtgcttat ccagtatggc tttcatttct
      901 ttccatttgt ctttgggtac cgaaaaggtc atgcgctcct gtgactctga aatccatatc
      961 tgccacggag tgagtcccgg atatttgagc ggaacggtgg agagatccac taccgcgccg
     1021 ccgcactctt ctgccatttc tgctaccgag caggaaagac cacctgcacc gttatccgta
     1081 acagaggtat agaggttttt atcacggagt tctcgcacga tagcatcaga gaatttcttt
     1141 tgtgttatcg cgtctccaat ctgtactgcg gtagcaggac tgtccgcact cagcgcttca
     1201 gaggagaagg tggcaccatg aatgccgtca gcacccacgc gtcctcccgc catcacaatg
     1261 taatcaccag gacgcgcctt tttcttatga aggagacgac ccccactctt acgaggaatg
     1321 agaccgatgg tgcccgcaaa cacgagtggt ttcccgcgat agctttcgtc cgcatacaca
     1381 aacccgagcg gcgtcggtat ccccgactga ttaccacccg cttcaattcc tttcactacc
     1441 ccttcaagaa tgcgctgagg cgaaagcatg ggacgtgtct ttgctgcatc gcgatacaga
     1501 ggacgcgtat cggatggttc acctacacaa aaaccataga ggttcgcaat gggttttgcg
     1561 ccaagcccaa acccgagtgc atcacggttc acgcctacaa tgcccgtgat agcacctccg
     1621 tacggatcaa gcgctgacgg ggtgttatgc gtctccatct tatgcgtgac gaggtagtca
     1681 tcattaaagg agatcgctcc tgagttatct ttaaataccg aaacgcagaa atccttcatt
     1741 cccttctttg ctcgaacttt ctccgttgca ccacgaatgt agcgcttata aataccctct
     1801 tctatctcat cgagcggatc attaaatatg gtgtgcttac agtgctcaga ccaggtctgt
     1861 gcaagactct caagctcaac gtccgttggc gcgcgtttct tcgaacggaa gtagtcgcgt
     1921 atggcatgta actcttcaag ggaaagcgcg agaggaccac gccgcgtacc atccttatcc
     1981 tcaattccct ccttaccaat gcgtgcaagc tcagcgtcag gtatctcaag atttaccgca
     2041 cgcgagggct cgggccgcgg cagcttcacc ttaggaacga tgagggcgac acgaccattg
     2101 ttccagtctt taaaggcagt gatgcgagcg cgctcgataa gcgggttatg cagctctcct
     2161 gccatgagcg tggcgtcagc tttagtgacc gttccttgaa gaaat
//
LOCUS       DAORIN010000095         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_14763_length_2185_cov_5.459155, whole genome shotgun sequence.
ACCESSION   DAORIN010000095 DAORIN010000000
VERSION     DAORIN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14763_length_2185_cov_5.459155"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..604)
                     /locus_tag="PK109_03420"
     CDS             complement(29..604)
                     /locus_tag="PK109_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRH24611.1"
                     /translation="MQTEWEAAFWDIDKDEMRERLKALGATLVYPERLMRRVNLYLPE
                     GNPIKGFARVRDEGDRITMTVKDMSGTAIEEQREAEVVVDDFDRAQDVLRALGCKDKN
                     YQETKRELWHLNGTEVTLDEWPFIEPIIEIEGHSEAEVKEVAALLGFDWKDAHFCSAD
                     KMYMERYGVTKEFVNIEIARLTFDIPNPFQK"
     gene            complement(610..1860)
                     /gene="tyrS"
                     /locus_tag="PK109_03425"
     CDS             complement(610..1860)
                     /gene="tyrS"
                     /locus_tag="PK109_03425"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRH24612.1"
                     /translation="MNLAEELKARGVGRESSAPLEKILETKRGIYLGMDPTADSLHVG
                     HLVPLMLMKRLADAGHRAVFLVGGGTGMIGDPRDSGERSLLDKRTLEHNKRALRAQLQ
                     KILGRKIEMVDNASWLGKLGLLEFLRDTGKHFSVNDLVKRDLIKRRLETPDESISFTE
                     FSYALLQGFDFLTLYKKKGVSVQVGASDQWTNMLSGVDLIRRKQAEEAFCFTCPLVTD
                     ANGKKFGKSEGNAVWLDGKKTSPFKFYQFWIGLPDEGLETYLKLYTFLPVSEIDALME
                     KHRGAPQDREAQETLARLVTEIVHGPAATAQAAAASDALFGGRPLSQLSREERAVLMG
                     EAVTVTVSKADASKSYPIADALVAGNLASSKSEARRLIEGKGVSINGKPVDSVDPTIR
                     TADFDGGLAILRKGKRDMVVLVLK"
     gene            complement(1876..>2185)
                     /locus_tag="PK109_03430"
     CDS             complement(1876..>2185)
                     /locus_tag="PK109_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24613.1"
                     /translation="TTYAPERYIYRLQETMPAVDLSSDQKSAIAALHTFLADGPKTGE
                     QLHERLHALKTEVPIAPKDLFTALYRIFLNRDSGPKAGWFLSVLPHEFVLARLNEAKG
                     "
BASE COUNT          478 a          580 c          547 g          580 t
ORIGIN      
        1 catctaggcc gattctagcc tattttcgtc atttttggaa cggattcggt atgtcgaagg
       61 tcagacgcgc tatttcaatg tttacgaatt ctttcgtgac gccgtagcgc tccatgtaca
      121 ttttatcggc agagcagaag tgcgcatcct tccaatcaaa tccgagaagc gcagctactt
      181 cttttacctc cgcttccgag tgtccttcga tttctataat aggctcaata aagggccatt
      241 cgtcgagcgt aacttcagta ccattcagat gccaaagttc acgcttcgtt tcctgatagt
      301 ttttatcttt gcagccgagt gctcgtagta cgtcttgggc gcgatcaaag tcgtcaacga
      361 ctacctccgc ttcccgctgc tcctctatag cagtgccgga catatctttt accgtcatgg
      421 taatgcgatc tccctcatcg cggacgcgag caaatccttt tatcggattt ccttcgggaa
      481 gatacaaatt cacgcgacgc attaaacgtt cgggatacac gagagtggcg ccaagagctt
      541 tcaagcgctc acgcatttca tccttatcaa tatcccagaa cgctgcttcc cattctgtct
      601 gcatcgaagc tatttaagta ccaggacgac catatcgcgc ttccctttgc gcagtatcgc
      661 tagtccgcca tcaaagtctg cggtgcggat cgtgggatca actgaatcga cgggctttcc
      721 attgatagag acaccctttc cttcgataag gcggcgtgct tcagacttag aggacgccag
      781 gtttccggca acaagggcgt cagcaattgg gtagctctta ctggcatctg cttttgatac
      841 ggtcaccgtt actgcttctc ccataaggac ggcgcgttct tcgcgggaga gttgtgataa
      901 cgggcgtcca ccaaacaacg catcgcttgc tgctgcggcc tgcgctgttg ccgctgggcc
      961 atgcacaatt tcagtaacga ggcgcgcaag cgtttcctgc gcttcccggt cctgtggcgc
     1021 gccacggtgt ttctccatga gcgcatctat ctctgaaact ggaaggaagg tatagagctt
     1081 gaggtaggtt tcaaggccct catcaggaag acctatccag aactgataga acttgaatgg
     1141 tgaggttttc tttccatcaa gccagaccgc attgccttcg gactttccaa actttttacc
     1201 gtttgcatct gttacgagtg ggcatgtgaa gcaaaaggct tcttcggctt gcttacggcg
     1261 tatgaggtcg actcctgaga gcatattggt ccactgatcg cttgccccta cctgcaccga
     1321 cacccctttc tttttgtaga gggttaagaa gtcaaagccc tgaagcagtg cgtacgagaa
     1381 ctccgtaaag gagatgctct cgtctggtgt ttcaagtcga cgcttaatga gatcgcgctt
     1441 cactaaatca tttacggaga agtgtttacc ggtatcgcgc aagaattcaa gaagtcccag
     1501 ttttccgagc cagcttgcgt tatctaccat ctctatcttt cttccgagta tcttttgaag
     1561 ctgcgctcga agtgcacgct tattgtgttc gagtgttctt ttatcaagaa ggctgcgctc
     1621 tccagaatct cgcgggtccc caatcatgcc tgtaccgccg cctacgagga acacggcgcg
     1681 atgccctgcg tctgcaagac gcttcattaa catgaggggg acgaggtggc cgacgtggag
     1741 cgagtcggca gtaggatcca taccgagata gataccgcgc ttcgtctcca gtatcttttc
     1801 aaggggtgcc gatgactccc tccccactcc gcgtgctttc aattcctcag ctaggttcat
     1861 agagctgagt ataacttatc ccttcgcttc gttcagacga gcgagtacaa actcatgcgg
     1921 aagcaccgac aaaaaccacc ccgccttagg tcctgagtca cggttgagga atatacgata
     1981 aagagcagtg aagagatctt tcggcgctat cggtacctca gtcttcagtg catggaggcg
     2041 ttcgtggagc tgttctccgg tctttgggcc gtccgctaag aaggtgtgga gcgcagcaat
     2101 ggcactcttc tggtcagatg agaggtcaac cgcaggcatg gtttcctgaa ggcgatagat
     2161 gtagcgttcc ggcgcatagg tggtg
//
LOCUS       DAORIN010000096         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_15125_length_2157_cov_4.320647, whole genome shotgun sequence.
ACCESSION   DAORIN010000096 DAORIN010000000
VERSION     DAORIN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15125_length_2157_cov_4.320647"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /gene="groL"
                     /locus_tag="PK109_03435"
     CDS             complement(<1..1515)
                     /gene="groL"
                     /locus_tag="PK109_03435"
                     /inference="COORDINATES: protein motif:HMM:NF009488.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRH24614.1"
                     /translation="MAKQILFGEDARKKIHDGMAKAAHAVKVTLGPRGRNVILDKGYG
                     GPRITNDGVSIAKEISFKDKFENMGAEIVKEVANKTNDGAGDGTTTSVVLLEALLDEG
                     LERVVKGGNAMAIRSGMEAAKNAAVAELKKMAKEVTKKSEYMQVASISAESEDLGKII
                     AETIEKVGASGVVTVEESQGMELSHEVVEGLQIDKGYVSAYMVTNPERMEAEMKDAQI
                     LITDKKIGAVQEILPLLEKIAAAGKRELVVIADDVEGEALTTFVLNKLKGTFNVLAVK
                     APGYGDRKKEMLADIATVVGAQVISADTGLTFENATVGMLGKAARVVATKDTTVFVGG
                     KGKKADIASRVDQLKALMDSATSKFDKEKFEERIAKLTGGVAVIRVGAATETEMKYLK
                     DKIDDAVKATKAAMEEGIVPGGGTALAKVSKKLAGASLKMSSDEQVGYDIVVRALEQP
                     LRQIALNCGKDDASVIVSKVQSGKASAGYDAMKDEIVEDMLEAGIIDPVKVTRGA"
     gene            complement(1517..1828)
                     /locus_tag="PK109_03440"
     CDS             complement(1517..1828)
                     /locus_tag="PK109_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRH24615.1"
                     /translation="MATKLDLKIEPLGDRVVVKPEKEEKVTASGIILADTGGKEKPEK
                     GTVVAVGPGRHNEDGDGRIPMDVKKGDKVYFKKPWDEPIKIDGVEYYVLSESDITFIV
                     K"
BASE COUNT          435 a          625 c          514 g          583 t
ORIGIN      
        1 ggcgccccgc gttactttca ccgggtcaat gatgccggcc tcgagcatgt cttcgactat
       61 ttcatccttc atggcgtcat agccggcaga tgccttgcct gactgcactt tactgacgat
      121 gactgacgcg tcatccttgc cgcagttgag tgcgatctga cgaagtggct gctcaagtgc
      181 acgcaccacg atgtcgtatc ccacctgctc gtcggaagac atcttgaggg atgctcctgc
      241 aagtttcttc gacactttcg caagcgcggt accgccgcca ggcactatac cttcttccat
      301 tgcggccttc gtcgctttga ccgcgtcatc tatcttgtcc ttcaaatact tcatctcggt
      361 ctcagtcgca gctcctacgc gaatgacagc cacacctccc gtgagctttg cgatgcgctc
      421 ttcaaacttc tccttatcga acttagaggt cgcgctatcc atgagtgctt tgagttgatc
      481 gacgcgagac gcaatgtctg ccttctttcc tttgccaccc acaaacaccg tggtgtcttt
      541 ggtagcaacg acgcgtgctg ctttgccgag catgcctacg gttgcgttct caaaggtaag
      601 gcccgtgtca gcggagataa cctgtgcgcc gacaaccgtt gcgatatcgg caagcatctc
      661 cttcttacga tccccgtagc cgggtgcctt taccgcaagc acgttgaagg tgcccttgag
      721 cttgttcagg acgaatgtgg tgagtgcttc gccctcaaca tcgtcagcaa tgacgacgag
      781 ctcacgcttg ccggctgcag caatcttttc aagaagcgga agtatttcct gcacggcacc
      841 aatcttctta tccgtgataa ggatctgggc atctttcatc tccgcctcca tgcgctctgg
      901 gttcgtgacc atgtacgcag acacgtatcc tttatcaatc tgaagacctt cgacgacttc
      961 gtgggaaagc tccataccct gggactcttc aacagtgacc acgcctgacg ctcctacttt
     1021 ctcaatagtt tcagcaatga tctttcctag gtcttcgctc tcagcagaga tggatgccac
     1081 ctgcatgtac tcagacttct tggtgacttc cttcgccatc ttcttaagtt cagcgaccgc
     1141 tgcattcttg gccgcttcca tgccgctacg aattgccata gcgttaccgc ccttcacgac
     1201 gcgctcgagt ccctcgtcga gaagcgcctc aagaagcacg actgacgtag tggtaccgtc
     1261 gcccgcgcca tcattcgtct tattcgcgac ttccttcact atctctgcgc ccatattctc
     1321 gaacttatcc ttgaaggaga tttctttagc aatggagacg ccgtcgttgg tgatgcgggg
     1381 accgccgtat cccttatcaa ggatgacgtt tcgtccacgg ggaccaagcg tgacctttac
     1441 cgcgtgcgcg gctttggcca taccatcatg gattttcttg cgagcgtctt cgccgaacag
     1501 aatttgcttt gccatattat ttaacgatga aagttatatc tgattccgag agcacgtagt
     1561 actcaacgcc gtctatcttt atcggttcat cccacggttt cttgaaatac accttgtcac
     1621 ctttctttac gtccatcggg atgcggccat cgccgtcttc gttatggcgg ccaggaccta
     1681 ccgcgactac cgttcctttt tctggctttt ccttcccacc ggtgtccgcg aggataatgc
     1741 cagacgcggt aaccttctct tccttctcag gcttaacgac gacgcggtcg ccaagcggct
     1801 ctatcttcaa atccaatttt gttgccatag attacaaaag ttagcttagt aaaagctttg
     1861 agagtttgcc cggttcttaa gcgcgtaagc gcccgaactc gtggctaaag tatacggagg
     1921 ctaccgtagc gctacaatat tgactttgct ataaaaagag gcattgtgta accacgttgt
     1981 gacggggacg ctaccgcccg ttagcagagg gagaggcccg atgcgtaaga ttgtacttac
     2041 tgttggtgta gtgctggcaa tttgctttgc tggcaatgtt cacgctcagt cactcagcac
     2101 gtacgcggaa tcgaatgcgg aatgccaagt gaatgaactg gcgggctggc gtgtact
//
LOCUS       DAORIN010000097         2096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_15926_length_2096_cov_3.950024, whole genome shotgun sequence.
ACCESSION   DAORIN010000097 DAORIN010000000
VERSION     DAORIN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15926_length_2096_cov_3.950024"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK109_03445"
     CDS             complement(<1..220)
                     /locus_tag="PK109_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRH24616.1"
                     /translation="MAQNYFLYARKSTEDDDKQVMSIEAQLFELREYARRENFEILEE
                     FTESKSAKTPGREVFGDMMQKIETGEAGG"
     gene            complement(395..466)
                     /locus_tag="PK109_03450"
     tRNA            complement(395..466)
                     /locus_tag="PK109_03450"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(431..433),aa:Glu,seq:ctc)
     gene            complement(489..1775)
                     /gene="murF"
                     /locus_tag="PK109_03455"
     CDS             complement(489..1775)
                     /gene="murF"
                     /locus_tag="PK109_03455"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRH24617.1"
                     /translation="MKQLLKSPVAGFLALLARFVLMKYRPIVVMVTGSVGKTSTKDAV
                     AAALSPKFNLRASEKSYNSEFGVPLTIIGAKNPWNDPLAWIKVIGEAFALIFLPNHYP
                     RLLVLEVGADRPGDLKKILAIATPDAVVVTRLPEVPVHVEAYASPAAVREEEFAPAYA
                     LAPHTPLIISADDSFAKHLASRLDARITTYGFAKDADIRIEGEMLRTEDPGPLGMHAQ
                     VVVGENHYDLSAQGALGRQQLYAPAAALACALSLGLSTKEALKGLEDYLPPPGRSRLL
                     AGVSNAILIDDTYNSSPAAVEEALRAISLIKAKRRIVVLGDMLELGRYSTHEHERIGT
                     LAAHAADIVVGVGARAISITEAARKKGKSEKEALHYASAQDAVDGLRDVLHPGDVVLI
                     KGSQGVRMERIVKELLKDANDSRLLVRQDPEWLKKA"
     gene            complement(1772..>2096)
                     /locus_tag="PK109_03460"
     CDS             complement(1772..>2096)
                     /locus_tag="PK109_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRH24618.1"
                     /translation="QTTQMLVSVVDKSLANGSVRIPEMSVAAKTGTAQIAGPGGKYAP
                     GLYFHSFFGYFPAYDPKFIILLYTKEPQGVQYASETLTHPFMDLTHFLINYYAIAPDR
                     ADTTQ"
BASE COUNT          479 a          542 c          557 g          518 t
ORIGIN      
        1 tgccccctgc ttcgccggtt tctattttct gcatcatgtc gccgaacacc tcacgccccg
       61 gagtctttgc gcttttcgat tcggtaaact cctctagaat ttcaaaattt tctctacgcg
      121 catattctcg caactcgaac aattgtgcct cgatagacat gacctgcttg tcatcgtctt
      181 ccgttgattt gcgagcgtag agaaaatagt tctgcgccat acgttttcaa atatagcacc
      241 ttgaaaacgt atggctcgat atacaatgca aatccccatt tttcggattc tccccgccgt
      301 cagaaaatac cgcgcgtctg cgcgcgaatt cgggtttgaa ttttgtggat acaggggcgg
      361 attccgagtt ggattcggag gaagttttat tttgtgactc cacggggaat cgaaccccga
      421 tttccagcgt gagaggctag cgtcctaacc gttagacgat ggagccaatt cggggagtat
      481 agcctgaatt aggccttttt caaccactca gggtcctgac gcaccaagag acgggaatcg
      541 ttcgcgtcct tcaataactc cttaacgatg cgctccatac ggactccttg cgatccttta
      601 atgagcacga cgtctccggg atgcagtaca tcgcggagtc cgtctacagc atcctgcgct
      661 gaagcgtaat gaagcgcctc tttctcactc ttccctttct ttcgtgccgc ctccgtaata
      721 cttatggcac gtgcaccaac accaacaaca atatctgcag cgtgcgcggc aagcgtaccg
      781 atacgttcat gctcatgtgt tgaataacgt ccgagctcga gcatgtcacc taagacaacg
      841 atacgccgct ttgctttgat taatgaaatg gcacggagtg cttcctcaac cgcagcaggg
      901 cttgagttat acgtgtcatc gataaggatg gcgttactca caccagcaag caatcgtgaa
      961 cgtccgggag gcgggagata gtcctcgagg cctttaagtg cttctttggt ggagagacca
     1021 agtgagagag cacacgcaag cgccgcagct ggtgcgtaga gctgctgacg gccaagcgca
     1081 ccctgcgctg aaaggtcgta gtgattctct ccaaccacca cctgtgcgtg catcccgagc
     1141 ggtccggggt cctctgtgcg gagcatctct ccttctatac gaatgtcggc atcttttgca
     1201 aacccgtacg tcgtgatgcg tgcatcaagg cggctcgcaa ggtgttttgc aaaggaatca
     1261 tctgcagaaa taataagtgg cgtatggggc gcaagcgcgt aggccggcgc aaactcttct
     1321 tcgcgcaccg cagccgggct tgcgtacgct tctacgtgta ccggcacttc aggaagccgc
     1381 gtcaccacga cagcgtcagg agttgctatc gcaagaatct ttttcagatc tcctggacgg
     1441 tctgctccga cttcaagtac caaaagacgc gggtagtggt tggggaggaa aatgagtgcg
     1501 aatgcttcgc caatgacctt tatccacgca agcggatcat tccaggggtt cttcgcacca
     1561 ataatagtga gcggtacgcc aaactcgctg ttataacttt tctcacttgc tcgtagatta
     1621 aacttagggg aaagcgctgc tgctaccgcg tccttggtag aggtcttccc tacacttccc
     1681 gtcaccatca cgactatggg ccgatacttc atcagaacga agcgcgcaag aagcgctaaa
     1741 aagcctgcga cgggtgattt gaggagttgc ttcattgggt agtgtcagcc ctatcagggg
     1801 cgatggcgta gtagttgatg aggaagtggg tcaggtccat aaaggggtgt gtgagggtct
     1861 cggaagcgta ttgtacgcct tgtggctctt tcgtgtagag gaggataata aacttaggat
     1921 cataggctgg gaagtaccca aagaaggagt ggaagtagag gccaggagcg tatttaccgc
     1981 cagggccggc aatctgagcg gtacccgtct ttgcggctac tgacatctca ggaatacgga
     2041 ctgatccgtt tgcgagtgat ttatcgacta cggacacgag catttgcgtg gtctgt
//
LOCUS       DAORIN010000098         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_16278_length_2071_cov_5.218750, whole genome shotgun sequence.
ACCESSION   DAORIN010000098 DAORIN010000000
VERSION     DAORIN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16278_length_2071_cov_5.218750"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PK109_03465"
     CDS             <1..659
                     /locus_tag="PK109_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202185.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRH24619.1"
                     /translation="AKPKIDWINAKEFQEGKGLEKLAQYDGILVPGGFGETGIEGKIH
                     AIRYARENKIPYFGLCYGMQLAVIEIARNVLGIKEAHTVEINSATPHPIVDVMPEQKD
                     LIATGNYGGTMRLGVYTAELRKGTIARDAYGKDKVEERHRHRYEINPEYVKQLEEGGI
                     VFSGKSPDGVLMEIAELPREVHPFFLGTQFHPELQARPLSPHPLFTEFLKAAKHRAGK
                     "
     gene            661..1029
                     /locus_tag="PK109_03470"
     CDS             661..1029
                     /locus_tag="PK109_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24620.1"
                     /translation="MSEWTGKGGTLRTPETEAKYKEYKAQGGLTACPLCERKDKLKEF
                     TYWHVIENLFPYDHIARVHHMIVLNRHAPDAEFTQEERDELHEIKYGYAQDHYQFIFE
                     PVGRQKSIPSHSHIHLIIAK"
     gene            1031..1762
                     /locus_tag="PK109_03475"
     CDS             1031..1762
                     /locus_tag="PK109_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRH24621.1"
                     /translation="MALSLISINVEFDKHYDTVLPFLEKEAADVVCVQELFERDIARF
                     EAVCGPLVAFAPDALHHEDASEILGSALFSRLPATNVTEHYYVGSRETVRQDDGKSDV
                     GTHSLVAADIGSEGYRIATTHFMWTRDGQSTPEQLRTLDTLFGVLDSLNAFVLAGDFN
                     APRGNETFTRLASRYTDNIPRSYETSIDVEKHKTRDIPEARARVSKYMVDGLFTTPEY
                     TAKDVRLEFGLSDHAAIIATIEKAA"
     gene            complement(1793..>2071)
                     /locus_tag="PK109_03480"
     CDS             complement(1793..>2071)
                     /locus_tag="PK109_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRH24622.1"
                     /translation="MCRFAAKNLVANGYAKKALVSVAYAIGRIDPVMVEAFDDEGKNL
                     SDVVAKNFDFRPQAIIERLGLRNPIFRPTAAYGHFGVAGRPWEETIKI"
BASE COUNT          525 a          548 c          537 g          461 t
ORIGIN      
        1 gtgctaagcc aaagattgat tggataaacg caaaggagtt ccaggagggt aagggtctgg
       61 agaagctcgc gcagtatgac ggcatcctgg tgcccggcgg cttcggtgag accggtattg
      121 aaggcaagat tcacgctatc cgctacgcac gcgagaataa gattccgtac ttcggtctct
      181 gctacggcat gcagcttgcg gtcattgaga ttgctcggaa cgtcctcggt atcaaggaag
      241 cacacacggt agagataaac tcagcaacgc cgcaccctat cgttgacgtc atgccagaac
      301 agaaggacct cattgctacc ggtaactatg gtggcaccat gcgccttggg gtatataccg
      361 cagaactcag gaagggaacc attgcgcgcg acgcctatgg caaggacaag gtagaagaac
      421 gccaccgcca tcgctacgag ataaaccccg agtacgtgaa gcagcttgag gagggtggca
      481 tcgtcttctc tggaaagtca cctgatggcg tactcatgga aatcgctgag cttccacgcg
      541 aggtgcatcc attcttcctc gggacgcagt tccatcctga gcttcaggca cgtcctcttt
      601 ctccgcaccc gctctttact gaattcttga aagccgctaa gcatcgtgcg gggaaatagt
      661 atgagtgagt ggaccggcaa aggaggaaca ctccgtactc cggagactga ggcgaagtac
      721 aaggaataca aggcacaggg tggacttacc gcgtgccctc tctgtgagcg caaggacaaa
      781 cttaaggaat tcacgtactg gcacgtcatc gaaaatcttt tcccctatga ccatatcgct
      841 cgagtgcatc acatgatcgt cctaaaccga cacgcacccg atgctgaatt cacccaagaa
      901 gaacgagacg agctgcatga aataaaatat ggttatgctc aggatcacta ccagttcatt
      961 ttcgagccag tagggagaca aaaatctata ccatcgcatt cgcatattca tctcattatt
     1021 gcgaagtaac atggcgctct cccttatttc tataaacgtc gagttcgata agcactatga
     1081 caccgtgctc ccgtttctcg aaaaagaagc tgcagacgtg gtgtgtgttc aggagctatt
     1141 cgaacgagat atcgctcgtt ttgaggcggt gtgcggacca ctcgtcgctt ttgctcccga
     1201 tgccctgcat catgaggacg cctcggagat actcgggagc gcgctctttt ctcgcctccc
     1261 ggcaactaac gttacagaac actactacgt cggctcgaga gaaacagttc gccaagacga
     1321 tgggaagagt gacgtgggga cgcactcgct cgttgctgca gatattggaa gcgaaggcta
     1381 tcgcatcgcg acgacacatt tcatgtggac cagagatggt caatcaaccc ccgaacagct
     1441 ccgcacgctt gatactttgt ttggcgtcct tgattcactc aacgcattcg tgctcgctgg
     1501 tgactttaac gcgccacgtg gaaatgagac gtttactcgc ttggcgtccc gatacacgga
     1561 taacatccca cgttcatacg agaccagcat tgatgtcgaa aaacataaga ctcgtgatat
     1621 tcctgaagcg cgtgcacgtg tctcaaagta catggtagac ggactcttca cgactcccga
     1681 atatacagca aaggacgtgc gactcgagtt cggtctttct gaccacgcgg ctattattgc
     1741 gaccatagaa aaagccgcgt agaacgcggc tttttcgttc gcactaagaa gactagatct
     1801 taatcgtctc ttcccatggg cgtccggcaa cgccgaagtg gccataggct gccgttgggc
     1861 ggaagatagg gtttcgaaga ccaaggcgct cgatgattgc ctgcgggcgg aagtcgaagt
     1921 tcttcgcaac cacatcagaa aggttctttc cctcatcatc gaatgcctcc accatgactg
     1981 ggtcaatgcg accaatagcg tacgctacgg acacgagtgc cttctttgcg tagccgttgg
     2041 caacaaggtt ctttgcagca aagcggcaca t
//
LOCUS       DAORIN010000099         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_17276_length_2007_cov_3.632172, whole genome shotgun sequence.
ACCESSION   DAORIN010000099 DAORIN010000000
VERSION     DAORIN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17276_length_2007_cov_3.632172"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PK109_03485"
     CDS             <1..1063
                     /locus_tag="PK109_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRH24623.1"
                     /translation="ARVIMLTGDNPETALWFAKETGIAHAGERAYTAADFKDFTDEAL
                     LQLLREHSVFARVAPSDKLRIARVLTGAGEVVAMTGDGVNDAPALEAASIGVALGSGT
                     DVAKEASDLVLLNNNFAVITYAIGEGRRLRDNVKKIFAYLLSTNFSEIFVIVSALIVG
                     VPLPLLPTQIMWANLIEGGAMNVALAFEPLYPSAMKRSPKHPDIAKVLSKDLLKLIAC
                     VGVLTGLMLVTLHFYLYSRGLPEDELRTIMFGALSASSFAGALSLKSFGTRLWKLPLL
                     SNPILLLSLAGSIIMLLAALFVPFFQAIVHTVPVSLYDVGTIALAGIVNLALIEIAKE
                     LFFIGPARRVAKRATLAAV"
     gene            1072..>2007
                     /locus_tag="PK109_03490"
     CDS             1072..>2007
                     /locus_tag="PK109_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRH24624.1"
                     /translation="MDIFTVALVVLGLILVQGIGLYFILKRREPVAPPSNGDGMMLLQ
                     KQMETLERSLDSRMAASSTQIHETMRSQLGESSRLIKEITEELTSVKEIGRQTQTYAE
                     QLQSLQDLLKNPKQRGILGEYYLETVLKNVMSPEDYAIQYPFKNGEIVDAVIRINKKL
                     VPVDSKFSLDNYNRFVGTPVGSAERAAAEKAFVNDLKLRITETAKYIRPEEDTMDFAF
                     MFIPSEGIYYDLLTNQVGGGEEENLIQRAASKSKVIIVSPTSFLAYLQTVMQGLKALQ
                     IEQKAVEIQKRVGELVKHIDATNSFYQKLGTSLGTV"
BASE COUNT          475 a          550 c          513 g          469 t
ORIGIN      
        1 agctcgcgtc attatgctca ccggagataa cccggagacc gcgctctggt ttgcgaaaga
       61 aaccggcatc gcacacgccg gagaacgggc atacacggct gccgacttca aagacttcac
      121 ggacgaagca ctactccagc tcctacggga gcattcggtg ttcgcacgtg tagcgccaag
      181 cgataagtta cgaattgcgc gggtgctcac gggcgcgggt gaagtggttg cgatgacggg
      241 tgatggggtg aatgacgccc cggccttgga agcggcgtct attggagtgg cgctcgggag
      301 tggtaccgat gttgcgaaag aggcgtcaga cctcgtgctt ctcaacaaca acttcgcggt
      361 tattacgtac gccatcggtg aaggacgccg acttcgcgac aacgtgaaga agatatttgc
      421 gtaccttctc tcaaccaact tcagtgagat attcgttatc gtctcagcgc ttatcgtagg
      481 cgtgccactt ccactccttc caacacaaat catgtgggca aaccttatcg agggcggcgc
      541 tatgaacgtg gcactcgcct ttgaaccgct ctacccgtct gcgatgaagc gctccccgaa
      601 gcatcccgat atcgcaaaag tgctctcgaa agatcttctg aaactcattg cgtgcgtcgg
      661 cgtccttacc ggtctcatgc tcgtcacgct ccatttctat ctctactccc gtgggcttcc
      721 tgaggatgag cttcgtacca tcatgttcgg tgcactttcc gcaagctcat ttgcaggcgc
      781 gctctcactt aaatcattcg gtacacgcct ctggaagctt ccgcttctct ctaaccctat
      841 cctcctcctc tcactcgctg ggagtatcat catgctattg gctgcactct tcgtgccgtt
      901 cttccaggca atagtgcata ccgttcccgt ctccttgtat gacgtcggta ccatcgctct
      961 tgcaggcata gtgaacctgg ctcttattga aattgcgaag gaactcttct tcataggtcc
     1021 cgcacgacgt gtcgctaagc gtgcaacctt ggccgcggta taatagggca aatggatatc
     1081 tttacggttg cccttgtggt gcttggtctt attctcgtgc agggaatagg gctctatttc
     1141 atcttgaaac gtcgcgaacc agtcgcaccc ccttcaaacg gcgacggcat gatgctcctc
     1201 cagaaacaga tggagaccct ggagcgctcc ctagattccc gcatggcagc gtcctcaacg
     1261 cagatacatg agacgatgag gagccaactg ggggaatcct cacgcctcat taaagagatc
     1321 acggaagagc ttacgtctgt gaaagaaata gggcgccaga cccagaccta tgctgaacag
     1381 ctgcagtcgc ttcaggatct tttgaagaat ccaaagcagc gcggcatcct cggcgaatac
     1441 tatctggaga ccgttctcaa aaacgtgatg agccctgagg actacgctat tcagtatcca
     1501 ttcaaaaacg gtgagattgt ggacgccgtt attcgtatta ataagaagtt ggtgccggta
     1561 gactccaagt tctccctcga taactacaat cgttttgtag ggacgcccgt aggaagcgct
     1621 gagcgcgcgg ctgccgagaa agcattcgtg aatgacttga agctccgtat tacggaaacc
     1681 gcgaagtaca ttcgtccgga agaagacacc atggacttcg cgttcatgtt cattccgtct
     1741 gaaggtatct actacgacct tctcacgaac caagtcggcg gtggagaaga ggagaacctc
     1801 atccagcgcg ccgcgtctaa atcaaaggtc atcattgtat cgcccacctc attcctcgca
     1861 tatctccaga ccgtgatgca gggattgaag gcacttcaaa ttgaacagaa agcggtagag
     1921 atacaaaagc gggtagggga actcgtgaag cacatagacg caacgaacag tttctatcaa
     1981 aagcttggca cttcgcttgg aacggta
//
LOCUS       DAORIN010000100         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_17709_length_1979_cov_5.211019, whole genome shotgun sequence.
ACCESSION   DAORIN010000100 DAORIN010000000
VERSION     DAORIN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17709_length_1979_cov_5.211019"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1426
                     /locus_tag="PK109_03495"
     CDS             68..1426
                     /locus_tag="PK109_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRH24625.1"
                     /translation="MTFGGRYAATLLFFGDIVIFFASLWLTLLIRYGAVPSDGLLADH
                     LFWFSILFAFWLLVFYMAGLYGKRVLLFKSSLFAVILQTQIANIVLAALFFFFVPSIG
                     IAPKTNLLLYLIISLALVMLWRLWIFPRITRPTSRERAALIGSGPEAQELAAEVNGNA
                     RYHLSFAVLATPAELEADFAGFKQRLKEAGVSLLVVDAERDAMRPLLPKLYELAFVQP
                     GYPFLDFHEVYEEVFDRVPLSLLEYDWFLKNLSGTGETFYLAAKRTIDIVGGLLMGLI
                     TVIGTPFVALALRLEGPGPVFITQERIGRYGERVRAYKFRSMRYSDEGKWKGEGENKV
                     TRVGALLRLTSFDEFPQFLNVLKGDVSLIGPRNDIRALGERLGEAIPYYNMRYIVQPG
                     ITGWAQINQRYEPGNISPQSIEETKMRLAYDFYYIKNRSLALDIIIALKTVKRMLFRV
                     AS"
     gene            1450..>1979
                     /locus_tag="PK109_03500"
     CDS             1450..>1979
                     /locus_tag="PK109_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRH24626.1"
                     /translation="MAKHTVLITGAAGYVGGMLVRAIAAREDVERIIGLDKEPLPDFI
                     KGTEKLTYLQMNTADEWEGEVRKYAPDTIIHTAWQIREIYGNRPLSWKWNIDGSEKVF
                     AFALNERQVKRLVHYSTVASYGAFPENTIEHRYTELEPFRTTDYLYAEEKRVAEERLE
                     AQYKKRTNAATVAIIRP"
BASE COUNT          443 a          544 c          514 g          478 t
ORIGIN      
        1 gcgctcgggg agtcgttcat gggacgtaac gttcttccta tgatataaca tcggaaagcg
       61 cccctccatg actttcggcg gacgatatgc agcaacgctc ctcttcttcg gggatattgt
      121 catattcttc gcctctctgt ggcttaccct ccttattcgc tatggggcag tcccaagcga
      181 cggactcctg gccgaccacc tgttttggtt tagtatcctg ttcgcgttct ggctcctcgt
      241 cttctatatg gcgggccttt acggcaagcg cgtgctgctt ttcaagagtt ccttgttcgc
      301 ggtcattctc cagacgcaga tagcaaacat tgtgcttgcc gcactgttct tcttcttcgt
      361 gccatcaatt gggatagcgc cgaaaacaaa ccttctcctc tatctcatca tctcgcttgc
      421 gctcgtgatg ctctggcgcc tttggatatt tccgcgcatc acgcgcccaa cgtcgcgtga
      481 gcgtgccgca ctcatcggtt caggacccga agcgcaggaa cttgccgctg aagtaaacgg
      541 aaacgcccga taccaccttt cgtttgcggt acttgccacc cccgctgagc ttgaggcaga
      601 cttcgctgga ttcaaacagc gcctcaaaga agcaggcgta tcgcttttgg tcgttgatgc
      661 agagcgcgac gctatgcgcc cgcttcttcc aaaactctac gaactcgcgt tcgtgcagcc
      721 cggctacccg ttcctcgact tccatgaagt atatgaagag gtctttgatc gcgttccgct
      781 ctcacttctt gagtacgact ggttcctgaa gaacctttct ggtaccggag aaacgttcta
      841 tctcgccgca aagcgcacca tcgatattgt gggcggactt ttgatgggac tcatcacggt
      901 cattggcaca ccctttgtgg cgcttgccct tcgtcttgaa ggccccggac cggtattcat
      961 cacccaggaa cgtatcgggc gctacgggga acgggtacgt gcgtacaaat tccgcagcat
     1021 gcgctactca gatgagggga agtggaaagg agagggggag aataaagtga cgcgcgtggg
     1081 cgcgctcctg cgcctcacgt cgtttgatga gtttccgcaa ttcttgaatg tcctcaaggg
     1141 tgatgtctct ctcattggtc ctcgtaacga catacgagca ttaggggaac gactcgggga
     1201 agccatcccg tactacaaca tgcgctacat cgtgcagccg ggcatcaccg gttgggcgca
     1261 gataaaccag cggtatgagc caggaaacat tagccctcag tctattgaag agacgaaaat
     1321 gcgtcttgcc tacgacttct actacataaa gaatcgttct ttggcgctcg acatcatcat
     1381 tgctcttaaa acggtcaaac ggatgctctt tcgcgtcgcg tcctaaatta gcgtattcta
     1441 gggatatcta tggccaagca cacggtactc attacaggag cggcagggta tgtgggcggt
     1501 atgctggtgc gcgcaattgc tgcacgtgaa gacgttgagc gcatcattgg ccttgataaa
     1561 gaaccactcc ctgatttcat taaaggaaca gagaagctta cctatctgca gatgaacact
     1621 gcagacgagt gggaagggga ggtacgaaaa tatgctcccg atacgattat tcataccgca
     1681 tggcagatac gagagattta cggaaaccgc ccactttcgt ggaagtggaa cattgatgga
     1741 tccgagaaag tgttcgcatt cgcgttaaac gaacgccaag tgaagcgtct cgtgcactac
     1801 tcgacggtgg cgtcctatgg tgcgttcccc gagaacacca tcgagcaccg ctatacagaa
     1861 ctcgagccgt tccgtacgac tgactacctt tacgcagaag agaagcgcgt ggccgaagag
     1921 cgactcgagg cgcagtataa aaagcgcacc aacgccgcaa ctgttgccat cattcgtcc
//
LOCUS       DAORIN010000101         1931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_18500_length_1931_cov_3.199360, whole genome shotgun sequence.
ACCESSION   DAORIN010000101 DAORIN010000000
VERSION     DAORIN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18500_length_1931_cov_3.199360"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..464)
                     /locus_tag="PK109_03505"
     tRNA            complement(393..464)
                     /locus_tag="PK109_03505"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(430..432),aa:Asn,seq:gtt)
     gene            570..695
                     /locus_tag="PK109_03510"
     CDS             570..695
                     /locus_tag="PK109_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24627.1"
                     /translation="MGANKHGVSPKKIRKSPPKTKKRLAAKAVMLAKKALSKKRS"
     gene            complement(698..1258)
                     /locus_tag="PK109_03515"
     CDS             complement(698..1258)
                     /locus_tag="PK109_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24628.1"
                     /translation="MKYYLDFDRTIFDTPAFKRAVARRLPFKELWKQGKAAVVELFTH
                     SETDSRRRRFMRSLGTFLSHGRFFFTPDEVNAYLYPDVPAFLAAHDCTIVTYGVEAFI
                     RAKVASALTDLKVTDVVYTRHKKGPIIQRLTAQKENGPFTYVDDAVFQLVSVARYCPD
                     VTILEMRRDGLPGDGRWPVVRSLLDL"
     gene            complement(1255..>1931)
                     /locus_tag="PK109_03520"
     CDS             complement(1255..>1931)
                     /locus_tag="PK109_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRH24629.1"
                     /translation="IVFGGEGTIRSAAQASTMDGPLLIPLPGGTMNLLPKALYGDVPW
                     ETALLSALRDPVIRVVSGGKADQERFFIAAILGAPALWAKAREALREGDIVEALEEGK
                     DAIEHLFDSKVSYRFNELHEGTAEAISVVCPLISTTLADDRQVLEAAVIDVAGAGEVI
                     ELATAAAFGQWRDSRNVAIVNTKHVSVSATSHIPVILDGETVHVGKELEIEFLPEAFR
                     AIVPAA"
BASE COUNT          454 a          544 c          537 g          396 t
ORIGIN      
        1 gggcgagcgc cgagtccgcg aggcgcgaga gatcaggggt tcggatttcg ggcgagaata
       61 ggtacgagcg aaatcgtata ctagagccca cactaggttt gattccatag tccccaattt
      121 ttcccgcgcg gcggctaccg ccgcgtatac agggtgcgcc gttcctgaca ctttttgatt
      181 cagagaatac aaattcagcc caaagatttt ttgaagaaaa gggagcggat ttttgggcgg
      241 ggagagaatg ggaaatccta gcccgtagtg tgcttgcccc tcccacccat gcgcgcacct
      301 actccttcgc tgaaaacgta ataatcagcg aagctcccta gactgacaat aaaagacaaa
      361 aaggcgcttt cgcgcctctt cgtcctactg ggctccgcgg gtaggattcg aacctacgac
      421 caactcgtta acagcgagcc gctctaccac tgagctaccg cggaatgtat cgcacgcgcc
      481 ccgaagggcg taggggcatt ctagctgaaa actatgattc agcaatggta tgcttaggga
      541 cataccaatt agcagctagt catagaccta tgggagcaaa caagcacggc gtaagcccaa
      601 agaagattcg taagagccct ccgaagacca agaagcgcct tgccgctaag gcagtgatgc
      661 ttgcgaagaa ggcactcagc aagaagcgca gctaggccta gaggtcgagc aaggagcgca
      721 ccacgggcca gcgcccatct cccggaagcc catcgcggcg catctcgaga atggtaacgt
      781 ccggacagta gcgagctact gatacgagtt ggaacacggc atcgtccaca taggtgaacg
      841 ggccgttttc cttttgtgcg gtgagtcgtt gaatgattgg tcccttctta tgccgcgtgt
      901 acaccacgtc cgtgacctta agatccgtga gcgcgctcgc tacttttgca cgtatgaatg
      961 cttccacccc atacgtcacg atggtgcagt cgtgagcggc aagaaaggcg gggacgtcgg
     1021 ggtagaggta cgcgttcact tcgtctggcg taaagaagaa ccgcccatga gagagaaagg
     1081 taccaaggct ccgcatgaag cggcggcgac gggagtccgt ctctgagtgc gtaaagagct
     1141 cgacgactgc ggccttccct tgcttccaga gttccttgaa tggaagcctg cgcgctacgg
     1201 ctcgtttaaa ggcgggggta tcaaatatgg tccgatcaaa gtcgagataa tacttcatgc
     1261 ggcaggaacg atggcgcgga atgcttccgg gagaaactct atctcaagct ccttccctac
     1321 gtgtaccgtc tctccatcaa gaatgacggg gatgtgtgag gtggcagaca cgctcacgtg
     1381 tttggtgttc acgatggcga cattccggga gtcgcgccat tgtccgaatg ccgctgccgt
     1441 agcaagctca ataacttcac cggctccggc gacgtcaatc accgccgcct caagcacctg
     1501 ccggtcatcg gcaagagtgg tggagatgag cggacacacc accgatatag cttctgcggt
     1561 tccttcgtgc agttcgttaa accgatacga tacctttgaa tcaaacaagt gctctatcgc
     1621 gtccttccct tcttcaagcg cttctacgat gtcgccctca cgaagtgctt ctcgtgcttt
     1681 tgcccagagc gcaggagccc cgagtatcgc agcgataaag aagcgttcct gatctgcctt
     1741 ccctcctgac accacacgaa tgaccggatc acgaagcgcg cttagtaatg ctgtctccca
     1801 cggcacatcc ccatacaaag ccttcgggag gaggttcata gtcccgcccg gaagcgggat
     1861 gaggagcgga ccatccatgg tgctcgcctg agcggcgctg cgtatcgtgc cctcgccccc
     1921 gaacacaatg a
//
LOCUS       DAORIN010000102         1901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_18981_length_1901_cov_5.638678, whole genome shotgun sequence.
ACCESSION   DAORIN010000102 DAORIN010000000
VERSION     DAORIN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18981_length_1901_cov_5.638678"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /gene="rpsT"
                     /locus_tag="PK109_03525"
     CDS             <1..189
                     /gene="rpsT"
                     /locus_tag="PK109_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRH24630.1"
                     /translation="VLLGASKDVRKAIAAGGKATAESLSKAYQAIDKAAKRGIIKKNT
                     AARKKSRLAKALAAATKK"
     gene            complement(225..296)
                     /locus_tag="PK109_03530"
     tRNA            complement(225..296)
                     /locus_tag="PK109_03530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Arg,seq:ccg)
     gene            complement(328..402)
                     /locus_tag="PK109_03535"
     tRNA            complement(328..402)
                     /locus_tag="PK109_03535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(367..369),aa:Arg,seq:gcg)
     gene            complement(441..914)
                     /locus_tag="PK109_03540"
     CDS             complement(441..914)
                     /locus_tag="PK109_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24631.1"
                     /translation="MRRAPLFEWEGREYRFEEKSADWYWALGILATAGAIASILFGNI
                     LLALVIAAAAGAIAIAAAKHARVHHFAIYEDGVAIDDNLYAFDTMLHFSVLEYADETL
                     PPSLSLKTKHFLAPHLLIPIVGHDPVEIYEYISLHLPEGRHDESPMDHLVELFRL"
     gene            complement(951..1035)
                     /locus_tag="PK109_03545"
     tRNA            complement(951..1035)
                     /locus_tag="PK109_03545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(999..1001),aa:Ser,seq:tga)
     gene            complement(1040..1381)
                     /locus_tag="PK109_03550"
     CDS             complement(1040..1381)
                     /locus_tag="PK109_03550"
                     /inference="COORDINATES: protein motif:HMM:NF039180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HRH24632.1"
                     /translation="MWNPIEQGMFIAPAGTAFGFGGALVALLFLIIALWSLAWKLVAV
                     WHAARNKQRLWMVALILVNTVGVLEIIYLAWFRKDNNGVGSNDFYPFLKDFKNPLVHE
                     SAPAPEGDKEK"
     gene            1419..1859
                     /gene="ruvX"
                     /locus_tag="PK109_03555"
     CDS             1419..1859
                     /gene="ruvX"
                     /locus_tag="PK109_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRH24633.1"
                     /translation="MRYLGIDYGTKKVGIALSDEAGTMGFPHGIIPNDQKLMATVRAL
                     IAKETVDAIVIGDSKDFTGSANPVAQEARAFGEALHNETGLPIFYEVEMLTTQEARRS
                     IDGAHPHTVSDTARKTPKGSGARDAVDASAAALILTSYLSRTHD"
BASE COUNT          510 a          486 c          496 g          409 t
ORIGIN      
        1 gtccttcttg gtgcttccaa ggatgtccgc aaggctatcg ccgctggcgg caaggcaacg
       61 gcggagtccc tctcgaaggc ctaccaggcc atcgataagg cggcaaagcg cggcatcata
      121 aagaagaaca ccgccgctcg caagaagtcc cgcctcgcta aggcactcgc cgcagcaacg
      181 aagaaataag aaacaccccg cgaatgcggg gtgtttcttt atagtgctct cggctggaat
      241 cgaaccagca tcacctcctt cggagggaga tatcctatcc attgaacgac gagagcgctg
      301 agctgttata gcacgagacg aacgtgatgg tgctcctggt aggaatcgaa cctacattac
      361 aacctccgca aggttgcgtc ctatccattg aacgacagga gcaattgaat gactctagcg
      421 ttttaggccc gtattcgcaa ctaaagacgg aagagttcga ccaaatgatc cattggggac
      481 tcgtcgtggc gaccctcggg aaggtgaagt gaaatgtatt cgtaaatctc cactgggtca
      541 tgtcccacga tagggatgag caagtgagga gcaaggaagt gcttcgtctt cagggaaagg
      601 gaaggaggca gagtctcatc cgcgtactca agtactgaga aatggagcat ggtgtcgaac
      661 gcatagaggt tgtcgtcgat agcaacgccg tcttcgtata ttgcaaagtg atgcacccgt
      721 gcatgcttag ctgccgctat ggcaatagcg cctgctgcag cagcaatgac gagagcaagg
      781 agaatgttac cgaaaagaat agaagcaatt gctccggcag ttgcaagaat gccaagcgcc
      841 caataccaat cggcgctttt ttcttcgaag cggtactctc gaccctccca ttcaaataac
      901 ggggcgcgac gcatcactag ataatagcac ttactaactg tcgaattgtg cggaggaggt
      961 gagattcgaa ctcacggtac cttgcggcac tcctgttttc aagacaggtg caatagacca
     1021 ctctgccact cctccgtgtt tatttttctt tgtcgccctc cggtgccgga gcggactcat
     1081 gcaccagagg attcttgaag tctttcaaga aaggatagaa atcgtttgaa ccgactccgt
     1141 tattatcttt gcggaaccac gcaaggtaga tgatttcaag aacacctacc gtattcacaa
     1201 gaataagagc aaccatccaa agacgctgct tgttgcgggc agcatgccat accgccacca
     1261 gtttccaggc aagtgaccag agcgcaatga taaggaagag gagggcaact aacgcgccac
     1321 caaatccaaa ggcggtcccg gctggggcga taaacatacc ttgttcaatt gggttccaca
     1381 tatatatgga agatagcata tttggtacga tgggccatat gcgctatctc ggcatcgact
     1441 acggtactaa aaaggtggga atcgcccttt ctgatgaggc gggcactatg ggatttcctc
     1501 atggcattat ccccaatgat caaaaactta tggctactgt ccgtgcgctt atcgcaaaag
     1561 aaacagtaga cgctatcgtg ataggggact caaaggattt cactggaagc gcaaaccctg
     1621 tcgcacaaga ggcacgcgcc ttcggtgaag cgcttcacaa cgaaactggt ctcccgatct
     1681 tttacgaagt agaaatgctc accacgcagg aagcgcgtcg tagtattgat ggcgcgcatc
     1741 cgcataccgt atcagacacg gcgcgcaaaa ccccgaaagg gtcaggcgcg cgcgacgctg
     1801 tggacgcatc cgctgcagcg ctcattctta ccagttatct ttcccgcacc catgattagc
     1861 attgatgagt tttcaaagat tgaagtaaaa gtgggcaccg t
//
LOCUS       DAORIN010000103         1807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_20875_length_1807_cov_5.643836, whole genome shotgun sequence.
ACCESSION   DAORIN010000103 DAORIN010000000
VERSION     DAORIN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20875_length_1807_cov_5.643836"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK109_03560"
     CDS             complement(<1..599)
                     /locus_tag="PK109_03560"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HRH24634.1"
                     /translation="MPNNEHERIEAVLSATQPQDLTSAERARIVAAVEAGIPATPMPS
                     PYFPFAFTTPTMIPLALVLAFFFGVGGTAVASDAARPGDFLFPVDRAIEDLRLSLSHD
                     KEDLRIAFANERLNEFSSIVDEEFGDHLAGATLTEAEADIFTNETIVKLEAVDRKAVF
                     STDADTREEIIAAIRTRFDFTEADIDAVLVVETEDRASRA"
     gene            complement(592..1119)
                     /locus_tag="PK109_03565"
     CDS             complement(592..1119)
                     /locus_tag="PK109_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRH24635.1"
                     /translation="MTDEVSLQGLVERSVALDAAAFRALIETLDGKLFRYVSSRTNSR
                     EEAIDILQDTFIALWHALPRFEYRSDAGFYRFVYTITKRHLSKVGSTRSLPLPDPDML
                     PDDAFLQEAPRNADVALALKKLGDTARDIVVLRHWSEFSFKEIGAMLSMKEEAVRVRH
                     HRALSKLRELLPLYA"
     gene            complement(1177..>1807)
                     /locus_tag="PK109_03570"
     CDS             complement(1177..>1807)
                     /locus_tag="PK109_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRH24636.1"
                     /translation="GSITFARYLDAVKIADFAEITHLTLDIYDYTAATLPEDVARAKA
                     RLDAGDATVVTPHTDNLIAGYTYIKDVYEKPILILRVETPRPIYAQGTLTLDVYLLLG
                     GIALLVFGIIMIGVLDMLVTSRVERLSKDVEKINDAHDLTLHLSAGEPDEIGRLAATI
                     NQMLAWLTEAREGEAASRREIVNLLDELKKGKEQAEEMARILAKKAEEK"
BASE COUNT          409 a          482 c          478 g          438 t
ORIGIN      
        1 gccctgcttg cacggtcctc cgtctctacc accaatacgg catcgatgtc tgcttcggta
       61 aagtcaaagc gtgtacgaat agcggcaata atctcttccc gcgtatcggc atccgtcgaa
      121 aagaccgctt ttcgatctac cgcttcaagc ttcacgatag tttcgttcgt aaatatatcg
      181 gcttctgcct cggtgagagt cgctcctgcg agatggtcac caaactcctc atcgactatc
      241 gagctgaact cattcagtcg ttcgttcgcg aacgcgatac gaagatcttc cttatcatgt
      301 gagagcgaga gccggaggtc ctcaatagct ctatcaaccg ggaacaagaa gtcgccagga
      361 cgcgcggcgt ccgacgcaac cgccgttcct ccgacgccga agaaaaatgc gagcacaaga
      421 gcaagtggaa tcatagtagg ggtggtaaac gcgaacggaa agtacggtga cggcattggg
      481 gtagcaggga tacccgcttc aaccgcagcg actatgcggg cgcgttccgc gctcgtaagg
      541 tcctgcggtt gcgttgccga aagcactgct tctattcgtt cgtgttcgtt attaggcata
      601 gagaggtagt aattcgcgaa gcttggaaag tgcgcgatgg tgccggacgc gcaccgcttc
      661 ttccttcata ctgagcatgg caccgatttc tttgaatgag aactcagacc agtgccgaag
      721 taccacaatg tcccgtgccg tgtctccgag cttcttgagc gccaatgcaa cgtctgcatt
      781 gcgcggcgct tcttgtagaa acgcatcatc aggaagcata tcggggtccg gaagcggaag
      841 agaacgggta ctccctactt ttgagaggtg ccgtttcgtg atggtataca cgaagcggta
      901 gaacccggca tctgagcgat attcgaatcg aggaagtgca tgccagagag caatgaaggt
      961 gtcctgcagg atatctatag cttcttcgcg gctgttagtc cgtgaggaca catagcggaa
     1021 gagtttcccg tcgagtgtct caatgagggc gcggaaggct gccgcatcta gcgcgacact
     1081 tcgctccacg agcccctgta aggacacttc gtcagtcatg cgtaaaggat accctgtacc
     1141 cctacaatgg cgagtttcgt ctgtttcgtt acatccctac ttctcttctg ccttcttcgc
     1201 gagaatacgt gccatctcct cagcctgctc ttttcctttc ttaagctcat ccaaaagatt
     1261 cacgatctca cggcgcgatg ccgcctcccc ttctcttgcc tcagtgagcc atgcaagcat
     1321 ctggttaatg gttgccgcta accgtccgat ttcatccggt tcccctgctg agagatgaag
     1381 cgtgagatcg tgtgcatcgt ttatcttttc tacatccttt gaaaggcgct caacacgact
     1441 cgtaacgagc atatcgagta cccctatcat gatgatgccg aacacgagga gtgctatacc
     1501 accgagaaga aggtacacat caagggtgag cgtaccttgc gcgtagatag ggcgtggcgt
     1561 ttcaacgcgc agtatcagga tcggcttctc gtatacatct tttatatagg tatagccagc
     1621 aatcaggtta tcggtgtggg gcgtcactac cgttgcgtcg cctgcatcaa gacgggcttt
     1681 tgcgcgtgcg acatcttccg ggagcgtggc ggcagtgtag tcatagatat caagcgtgag
     1741 gtgtgtgatc tcagcgaagt ccgctatctt taccgcatct aaatatcgag caaaggtgat
     1801 tgaaccg
//
LOCUS       DAORIN010000104         1766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_21720_length_1766_cov_3.953244, whole genome shotgun sequence.
ACCESSION   DAORIN010000104 DAORIN010000000
VERSION     DAORIN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21720_length_1766_cov_3.953244"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..684
                     /gene="def"
                     /locus_tag="PK109_03575"
     CDS             118..684
                     /gene="def"
                     /locus_tag="PK109_03575"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRH24637.1"
                     /translation="MTIVQDGDAVLRKKAVPVTASEFGTKEFTKILNDMEQALDSQAD
                     GVALAAPQIGISKRIFIVRYDRMAPPPEEGEKERPVEVGVFINPEFVRKARKKAIMDE
                     GCLSVRGKYGQTKRHERATVTAQDASGKKFERGGGGILAQAFQHEIDHLDGILFIDHA
                     TEVHSVDDAGVRKSRRTEGWIPEDEAHA"
     gene            677..1516
                     /locus_tag="PK109_03580"
     CDS             677..1516
                     /locus_tag="PK109_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRH24638.1"
                     /translation="MHKPFVYFGTPYVARDTLAILVERGYTPALVVSTPDARRGRGME
                     LQPSETKAWATEKNIPVITPEKLTPVVIEEIRATKAEFAIVVAYGKILPLPIFDIFPN
                     GILNIHYSLLPKYRGASPVESALLAGETVTGVSIQKMVHELDAGDIVAAREVAITPTE
                     TTRELRPRLIQEGAELLIDILPSFEAGTLSSIAQDPAAVTYAKKIAKEEGQLDLSGDA
                     RTNWNKYRAYAESPGTFFYADKNGTPVRVKIKSATFVDGVFMPDRVVPEGKKEMAYAD
                     FVR"
     gene            complement(1513..>1766)
                     /locus_tag="PK109_03585"
     CDS             complement(1513..>1766)
                     /locus_tag="PK109_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRH24639.1"
                     /translation="EAVSPRVRKMTYVRVQVKPGSRKESVIQTDEKTYSISVKEPASQ
                     GLANDRVKQLIAEELGVKIQKIRLISGHRSPRKIFDIDY"
BASE COUNT          448 a          459 c          457 g          402 t
ORIGIN      
        1 ctgtccccta cccgccaggg tgcccctcgc tcacctcttt ctgccggctt cttaggaagt
       61 cggcttctta ttacctcctc tacacggaag tactacgtcg cggtatagtc cctttatatg
      121 acgatagtcc aggatggtga cgcggtgctc cgcaaaaaag cagtaccggt tactgcatca
      181 gaattcggta ctaaggaatt caccaagata ctcaacgaca tggagcaagc gctcgactcc
      241 caagccgacg gcgttgcact tgcagcgcct cagattggga ttagtaagcg catatttatc
      301 gtgcgttatg accgcatggc acctccacct gaagaaggcg aaaaggaacg ccctgttgaa
      361 gtcggcgtat tcataaaccc tgagttcgta cgaaaggcgc gcaagaaagc tatcatggat
      421 gaaggctgtc tttcggtccg cggaaaatat ggacagacca agcgccatga acgcgctacc
      481 gtcaccgccc aggacgcgtc aggaaagaag ttcgaacgcg gcggaggcgg gatacttgcc
      541 caagctttcc agcatgaaat agatcacctc gacggtattc ttttcatcga tcacgcaaca
      601 gaagtgcaca gcgtcgatga tgccggcgtt cgcaagtcac gccgcactga gggatggatt
      661 cctgaagacg aagcgcatgc ataagccctt tgtatatttt ggtacgccgt atgtggcgcg
      721 cgatacactc gccattctcg ttgaacgtgg ctatacgcca gcgctcgtcg tatccactcc
      781 cgacgcacgt cgtggacgag gcatggagct acaaccatca gaaacaaaag catgggccac
      841 agaaaagaat attccggtga taacacctga aaaactcact ccggtagtga ttgaggagat
      901 tagagcgacg aaagcagagt ttgctatcgt ggtcgcatac ggaaagattc ttcccctccc
      961 catattcgat atatttccaa atggcattct aaacatccac tactctctat tgcctaaata
     1021 tagaggcgct tccccggtag agtcagcgct ccttgcggga gaaaccgtga cgggggtttc
     1081 tatacaaaaa atggtacatg agcttgatgc cggtgatatc gtggcggcac gcgaggtagc
     1141 gattacccct acggagacga cacgggagct acgtccgcga ctcatacagg agggagcaga
     1201 gctgttaatt gatattttgc cttcgtttga ggcgggcacc ctctcttcaa ttgcgcagga
     1261 ccctgctgcg gtgacgtatg caaaaaagat agcgaaagaa gaaggtcagc tcgatcttag
     1321 cggagacgcc cgtactaact ggaacaagta ccgtgcctat gcggagtctc cgggtacgtt
     1381 cttttatgct gataaaaatg gaacaccagt gcgcgtgaag ataaagagcg ccacctttgt
     1441 cgatggcgtg tttatgcctg accgggtagt acctgaagga aagaaagaaa tggcgtacgc
     1501 ggacttcgtg cgctagtagt cgatatcaaa gatcttacgg ggagaacggt gccccgagat
     1561 aaggcgaatc ttttgaatct tcacgccaag ctcttccgca attaattgct tcacacggtc
     1621 attcgccaac ccttgagagg ctggttcttt tacggatatg gagtacgtct tctcatccgt
     1681 ttggataacg gattccttcc ggctccccgg ctttacctga acgcgcacgt aggtcatttt
     1741 ccgaaccctt ggagaaactg cttcac
//
LOCUS       DAORIN010000105         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_21822_length_1762_cov_4.727006, whole genome shotgun sequence.
ACCESSION   DAORIN010000105 DAORIN010000000
VERSION     DAORIN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21822_length_1762_cov_4.727006"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK109_03590"
     CDS             <1..220
                     /locus_tag="PK109_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRH24640.1"
                     /translation="EAMAQAAVDRWDGKEMDGRMLSVSFARPQGDRPPREGGFGGGNR
                     GGGRGGFGGGDRGGYGGNDRGGYGGGSY"
     gene            complement(266..583)
                     /locus_tag="PK109_03595"
     CDS             complement(266..583)
                     /locus_tag="PK109_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24641.1"
                     /translation="MSPENTNSEPGSPRYGYEDLSFEQMESHEVYGPIFQQAALNYLK
                     SADSEYFNREFSGELDSEGYLLRKDGTNSNTRPSQNISGFGLKVVESATKEEIGKRLG
                     KKN"
     gene            complement(603..899)
                     /locus_tag="PK109_03600"
     CDS             complement(603..899)
                     /locus_tag="PK109_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24642.1"
                     /translation="MPFKFELRPAQRAPLTVPGFEKVETAELREDALIINGVTHPLVE
                     WLYPVASNPTQIVEPTLIFKNTVTGQDTGIPLSLLEKTFGQKVPLPIGDTWPGK"
     gene            complement(903..1373)
                     /locus_tag="PK109_03605"
     CDS             complement(903..1373)
                     /locus_tag="PK109_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HRH24643.1"
                     /translation="MTTLKSAFWNIVFALFFAAVLLKGVFWLYEDGHLYRDIPVGDYI
                     LMALATFRLVRLFCYDSITKFMRDAVAHPKHDSFLGTLHSLLSCPWCAGLWFSFFVVF
                     GYYATPLAWPVLLILSIAGVGSFIQVLANLMGWHAEGKKREVLGANHGSTSTCG"
     gene            1396..>1762
                     /locus_tag="PK109_03610"
     CDS             1396..>1762
                     /locus_tag="PK109_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24644.1"
                     /translation="MALDPKDVEALRREKYGNNAAADMTADLARLRKGEPLAYVIGNI
                     PFLGLTIDLFSHPLIPRPETEWWVEELASHIGDKQFKVLDLCAGSGAIGLALLKLCPN
                     VQVSFGELMEEHDAVIKKNI"
BASE COUNT          415 a          455 c          480 g          412 t
ORIGIN      
        1 tgaggctatg gcgcaagctg ctgtagaccg atgggatggc aaggaaatgg atggccgtat
       61 gctctccgtt tctttcgctc gcccccaggg tgatcgccct cctcgcgaag gcggtttcgg
      121 cggcggtaac cgtggcggtg gtcgcggcgg cttcggcggc ggtgatcgcg gtggctacgg
      181 cggtaatgat cgcggtggtt acggaggcgg tagctactaa gctcccttca aagaaaaacc
      241 ccggcatatg ccggggtttt tctttttagt ttttctttcc aagtcttttt ccaatttctt
      301 ctttagtagc cgattcaaca actttaagac caaacccgct aatattctga cttggtcggg
      361 tatttgagtt cgtaccgtcc ttgcgaagca gatacccttc actatcgagt tcacctgaaa
      421 attctcgatt gaaatattca gagtctgcac tcttgagata attcagtgcc gcctgctgaa
      481 atataggacc gtacacttcg tgcgactcca tctgctcaaa actcaaatct tcgtatccgt
      541 accgaggaga accaggttcg ctgtttgtat tttctggtga cataagacaa tagtaaccta
      601 gattatttac ctggccaggt atcccctatc gggagcggca ctttctgacc gaatgtcttc
      661 tcaaggagcg atagcggaat gccagtatct tgaccagtta cggtattctt gaatatcaac
      721 gtcggctcta caatctgggt tggattgctt gccaccggat agagccactc tactaacggg
      781 tgcgtaacgc cgtttatgat gagtgcgtcc tcgcggagct cggcggtttc aaccttttca
      841 aatccaggca ccgtgagcgg cgcacgctgt gcaggacgta attcgaactt aaacggcata
      901 cgctatccgc aggtactcgt agagccgtgg ttcgctccta gcacctctcg tttctttcct
      961 tctgcgtgcc atcccatcaa gttcgcaagc acctggataa aggaaccaac gccggcgata
     1021 gagaggatga ggagtaccgg ccacgcaagc ggcgttgcgt agtacccgaa caccacaaag
     1081 aaagagaacc agaggccagc acaccacggg cacgagagaa ggctgtggag cgtaccaagg
     1141 aagctgtcgt gcttcgggtg cgcgactgcg tcacgcatga acttcgtaat gctgtcgtag
     1201 cagaagagac ggaccaagcg gaaggtggca agcgccatca agatgtagtc gcccaccgga
     1261 atatcacgat acaggtgacc gtcctcatag agccagaaga ctcccttgag gaggacggcg
     1321 gcgaagaaaa gggcgaacac gatgttccag aaggcagatt tgagcgttgt catgcaaaga
     1381 gtgtaccatc agggagtggc acttgacccc aaggatgtgg aggcgcttcg cagggagaaa
     1441 tacgggaaca atgctgcggc agatatgacc gcggacctag cacgcctccg taaaggcgag
     1501 ccgcttgcct atgtcattgg gaacattccc tttctcggac tcaccatcga cctcttctcc
     1561 cacccgttga ttccccgccc tgaaacagag tggtgggtcg aagaacttgc cagccatatc
     1621 ggtgacaagc agttcaaggt actggatctc tgtgcaggct ccggcgctat cggtcttgcc
     1681 cttctgaagc tctgtccgaa tgtgcaggta tcctttggtg aactgatgga agagcatgat
     1741 gcggttatca aaaagaatat aa
//
LOCUS       DAORIN010000106         1761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_21860_length_1761_cov_3.660610, whole genome shotgun sequence.
ACCESSION   DAORIN010000106 DAORIN010000000
VERSION     DAORIN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21860_length_1761_cov_3.660610"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1273
                     /gene="dnaX"
                     /locus_tag="PK109_03615"
     CDS             248..1273
                     /gene="dnaX"
                     /locus_tag="PK109_03615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRH24645.1"
                     /translation="MHQSLYRAYRPQKFSEVAGQDQVTGPLQEQLKTGTVAHAYLFSG
                     SRGLGKTSVARIFAAALHVSENDLYEIDAASNNSVDDIRALAENIYTLPFEGKYKLYI
                     LDEAHMLSKGAWNAFLKTLEEPPAHAIFVLATTELEKVPETVRSRCQVFEFKKPTRAG
                     LVKLVSSVAKKEGYTLEAGVADLVALLADGSYRDALSILEKIFSVSGNKTITRSEAEK
                     ATGAPKSALVAAFVEALGEGNADKALTAVRNTTDEDVDMALYLSLVLEQIRTVLLVRH
                     APELRKSLQVELGEEYAALEALAMNKESKLTHHTLTALLDASSRIRFSPVPALPIELA
                     VFELFGE"
     gene            1270..>1761
                     /locus_tag="PK109_03620"
     CDS             1270..>1761
                     /locus_tag="PK109_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24646.1"
                     /translation="MKAAVSKLQLLLALLFVGAAGYVLYTQAAPLLIPPCKEPIEYSV
                     GTVDPRFGISQSQYERALATAAAVWNTEAGRTLVTLGAGADTIPVSLEYSDVQRATEL
                     GKNIDAEQSSYDAKKAEVNALRDDFSAAKAQYERSAASYEKRTAAYEKEVAYWNSQGG
                     APTE"
BASE COUNT          439 a          471 c          466 g          385 t
ORIGIN      
        1 aacacctcag aggccgagct cattgctgaa cgtgatgcgt gccttgcaac agctcgcgct
       61 cgtgatgcgt atgcaagaag cgcgaaggat gataacggca gaatgctttt ctccaccggc
      121 gcacagtgcg cccgtcgaga tgctgccaga attcaagcgc gcatcaccta gctcaagtcc
      181 tctgcttgag aaaaacgcgt cagggtccgg cccgacgcgt ttttccatgc ctgttatact
      241 gtgcgctatg catcagtccc tctatcgcgc gtatcggccc cagaagttct ctgaggttgc
      301 tgggcaagat caggtcacgg gtccgctcca ggagcagctc aagaccggaa ctgtagcgca
      361 tgcctatctt ttctcagggt cgcgtggtct cggaaagacc tctgttgccc gcatcttcgc
      421 cgcagccttg catgtatcag agaatgattt gtatgagatc gacgcagcaa gtaataacag
      481 cgtcgatgat attcgcgcgc tcgcagaaaa tatctacacg cttccgtttg aagggaagta
      541 caaactttat attctcgacg aagcacacat gctctctaaa ggggcatgga atgcgtttct
      601 caaaacgctt gaggaaccac cagcacatgc catcttcgtg cttgcgacta ccgagcttga
      661 gaaagtacca gaaacggtgc ggagtcgctg ccaggtattt gaattcaaaa aaccgactcg
      721 cgctggtctc gtgaagctcg tctcgtctgt cgctaagaaa gagggataca cccttgaagc
      781 gggcgtagcg gatcttgtgg cgcttctggc tgatggctcc tatcgcgacg cgctttcgat
      841 actagagaaa atattttctg tttcaggaaa taagaccatt acgcgtagtg aagcagaaaa
      901 agcgacggga gcaccgaagt ccgccctggt tgcggcattt gttgaggcgc tcggggaagg
      961 aaacgctgac aaagccctta ccgcagtacg gaatactacg gacgaagacg ttgatatggc
     1021 gctctatcta tcgctcgtgc tcgagcagat acgcaccgtg cttctcgtgc gtcacgcgcc
     1081 agaactccgc aaatcacttc aggtagagct tggtgaggag tacgcggcac ttgaagcgct
     1141 cgctatgaac aaagaatcaa agctcacgca tcacaccctt acagcattac tcgatgcctc
     1201 atcacgtatc cgcttctcac cggtgccagc gcttcccata gagctcgcgg tgttcgaact
     1261 atttggagaa tgaaggcggc agtatccaaa ctacaacttc tgttagcgct cctctttgtc
     1321 ggagcagccg gatacgttct ctacacacag gccgccccac tccttattcc gccctgcaaa
     1381 gaacctattg agtattcagt aggaacagtt gatccacgct tcggtatttc acaaagtcag
     1441 tatgagcgag cgttagccac ggctgcagca gtatggaata ccgaagcagg gcgcacgctc
     1501 gtgacgcttg gagcgggagc agacaccatt ccggtatccc ttgaatatag cgacgtgcag
     1561 agggcgacag aactcgggaa gaacatcgat gccgagcagt cttcgtatga cgcaaagaaa
     1621 gcagaggtga atgcactgcg tgatgacttc tcagcagcga aagctcagta tgagcgttct
     1681 gccgcatcat atgaaaaacg cactgctgcg tatgagaaag aggtagcgta ctggaactcc
     1741 caaggaggcg ctcctacaga a
//
LOCUS       DAORIN010000107         1760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_21892_length_1760_cov_3.092082, whole genome shotgun sequence.
ACCESSION   DAORIN010000107 DAORIN010000000
VERSION     DAORIN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21892_length_1760_cov_3.092082"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1760
                     /locus_tag="PK109_03625"
     CDS             <1..>1760
                     /locus_tag="PK109_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRH24647.1"
                     /translation="PMTKFWSFFIHRHHFTVFLMIVLVIAGLYAVSTIPKESTPDIAI
                     PLGIVSTALPGASASDVERLVTDKIEDAVLSVEGVSKVTSSSGDGFSSISVEFVASAN
                     IEKSIQLLKDAVDTAKGDLPEEATDPVVSDVNFADQPILIVSVSGALAPAELTHLGQA
                     AKDEVERVPGVASVALSGVRAREVQVIISEAKLRQFGLTPSQVTGAIAAAGFGSPAGT
                     ITVDGIEYGVRFDAGISTTEDVENVYLQGAGAPIRVRDVARVIDGLEDPSSYSRVSVN
                     GTPANPSLTLTVSKSRGGNIVETAERVKDKLEEMRATGVLAGTDIVVSYDGAEEVDKS
                     LSELTKAGFETIVLVMLVLFATLGWREAFVAAASIPLSFLIAFIFMLATGNTLNNVSL
                     FSLILAIGILVDSGIVVVEAFHTRLKKLGAHDAAVAALREYAWPLIAGTFTTIVVFIP
                     LFFLSGIMGKFLASIPYTVIFVLLASIFVALGFVPLLSVMYVKDDTREEERRQERYNR
                     VAQAWYRDFLHGFLANKRNQNRFLWAMGLLFIVALALPATGLLKAILFPGDDFDYAYI
                     NIETPQGTDLAVTDLAVREV"
BASE COUNT          344 a          511 c          461 g          444 t
ORIGIN      
        1 cgcccatgac taagttctgg agcttcttca tacaccgcca tcacttcacg gtcttcctga
       61 tgatcgtgct cgtgatcgca ggcctctacg cggtctccac tatcccgaaa gaatcgacgc
      121 ctgatatcgc catcccgctt ggcattgtct ctaccgcgct ccccggtgca tcagcatcag
      181 atgttgagcg cctcgtcacg gacaagatag aggacgcggt gctttcggta gaaggagtat
      241 caaaagtgac gtcgtcgtcc ggcgacgggt tctcttctat ttctgtagag ttcgtagcgt
      301 ccgcaaacat tgagaaatcc atccagctcc tcaaagatgc ggtggatacc gctaaaggcg
      361 accttcccga ggaagccacc gacccggtcg tgtctgacgt taactttgcc gatcagccta
      421 tcctcatcgt ctcagtctca ggcgcgctcg ctccggcaga actcacgcac ttgggacaag
      481 ctgcaaagga tgaggtggag cgcgtcccgg gcgtcgcctc cgttgcgctc tctggggtgc
      541 gcgcacgcga ggtacaggtc attatttctg aagcgaagct tcgtcagttc ggtcttaccc
      601 cttctcaagt gacgggggct attgctgcgg cagggttcgg aagtcctgcc ggcaccatca
      661 ccgttgatgg gattgagtac ggcgtgcgct ttgacgctgg catctccacc accgaagacg
      721 tcgagaacgt gtaccttcag ggcgcaggcg ctcctattcg tgtgcgcgac gtagctaggg
      781 tcattgatgg cctagaagat ccctcaagct actcacgtgt gagcgttaac ggcacacccg
      841 caaacccgtc ccttaccctt accgtaagca agagtcgcgg tggcaacatc gtcgagacgg
      901 cagaacgcgt gaaggacaag cttgaggaga tgcgcgctac tggcgtcctt gcaggaaccg
      961 atatcgtcgt gtcgtacgac ggcgctgaag aagttgataa atcactctct gaactcacca
     1021 aggcagggtt tgaaactatc gtgctcgtta tgctcgtctt gttcgccacc ttgggctggc
     1081 gcgaagcatt cgtcgctgca gcctccattc ccctttcatt cttgatagcg ttcatcttca
     1141 tgctcgctac cggcaataca ctgaataacg tgtcgctgtt ctcccttatt ctcgctatcg
     1201 gtattctcgt ggactccggc atcgtggtgg tagaagcgtt ccatacccgt cttaaaaagc
     1261 ttggcgctca tgacgctgcc gttgctgcgc ttcgcgaata cgcgtggcca ctcatagccg
     1321 gtaccttcac gaccatcgtc gtgttcattc cgctcttctt cctttcgggc atcatgggta
     1381 agttcctcgc ttctatcccc tacacggtta tcttcgtgct tcttgcttct atctttgtgg
     1441 cacttggatt cgttcctctt ctttcagtca tgtacgtaaa agacgacacg cgcgaagagg
     1501 aacgccgcca ggaacgctat aaccgtgtgg cacaggcgtg gtaccgcgat ttcctccatg
     1561 gcttccttgc gaacaagcgt aaccagaatc gtttcctctg ggcgatggga cttctattta
     1621 ttgttgcgct tgcgcttccg gctacgggac tgttgaaagc aattcttttc ccgggagatg
     1681 actttgacta tgcctacata aacattgaga cgccgcaggg cacggatctt gcggtcactg
     1741 accttgccgt gcgtgaagta
//
LOCUS       DAORIN010000108         1757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_21978_length_1757_cov_2.789659, whole genome shotgun sequence.
ACCESSION   DAORIN010000108 DAORIN010000000
VERSION     DAORIN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21978_length_1757_cov_2.789659"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="PK109_03630"
     CDS             <1..1444
                     /locus_tag="PK109_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRH24648.1"
                     /translation="FDYAYINIETPQGTDLGVTDLAVREVEEVLYADPAIESFVTVVG
                     SGSSLSGGFEGAGSGSQIANITVNLPKGHKETSTEFVARLRDDLKSVDSAIITVGEPA
                     GGPPAASPIAITFSGDDLGALTTAADTAANTLSEIPNVINISASTKDSSAEFVVKLDS
                     AKAAEQGVSAPAVAEILRVALFSTKATSIRTGGDDIEVRAKLDLNPDYADPSQTTHVS
                     IDAVRSLTVNGANGPVPLASIATITYESAQHAIRHEAGTRIMSVSAEVAPGGNAIEAT
                     AEFNKRFTEDKLPAGVVIAQGGETEDVQKSFFELIIALFAGAALMLSILVLEFNSFRH
                     SFYLLLIIPLSLIGVHAGLTLMGQPLSLTSMLGVIALAGVIINHAIILMDSIARIHKE
                     RSELSLEEVVVEAAATRLRPILLTTVVTVVGMLPLAFASPFWAPLAFAIMFGLAFSLL
                     LTLVLIPMLYYRWPGKATRQHYDMAEQEPS"
     gene            complement(1463..>1757)
                     /locus_tag="PK109_03635"
     CDS             complement(1463..>1757)
                     /locus_tag="PK109_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24649.1"
                     /translation="DEYRSIERRAYRAYAALTSARRITETTGKEVIIAVNADDVRPEV
                     VADATVTTEAAPMLMKAATLMTEATTSTSTEMKRSNRMERALQFWVHDRTDKE"
BASE COUNT          344 a          532 c          468 g          413 t
ORIGIN      
        1 cttcgactat gcgtacataa acattgagac gccgcagggc acggaccttg gagttactga
       61 ccttgccgta cgcgaagtgg aagaggtcct ctatgccgat cctgccattg agtcctttgt
      121 gaccgtggtt ggttcgggaa gttccctttc gggcgggttt gagggcgctg gaagcggcag
      181 ccagatagcg aacataacgg tgaaccttcc gaaaggccac aaagagacga gcacggagtt
      241 tgtcgcgcgg ttgcgtgacg atcttaagtc ggtagactcc gccatcatta cggtaggaga
      301 gcctgcgggc ggcccgccgg cagcatcccc tatcgctatc accttctcag gggacgacct
      361 gggtgccctc actaccgccg ctgataccgc cgcaaacaca ctctcagaga ttccgaacgt
      421 cattaatatc tccgcttcta ccaaggactc aagcgcagag ttcgtggtga agcttgatag
      481 cgcgaaggct gctgagcaag gcgtgtcagc gcccgcagta gcggagatac ttcgcgtcgc
      541 actctttagc accaaagcaa cctctattcg caccggtggg gatgatatag aggtgcgcgc
      601 aaagctcgac ctgaatcctg actacgcgga cccgtctcag accacgcacg tctccattga
      661 tgcggtgcgt tccctaacgg tgaacggagc gaacggcccc gtgccgcttg cctctatcgc
      721 gaccataacg tacgaatccg cgcagcatgc tatacgccat gaagcgggta cccgcatcat
      781 gtcggtctca gctgaggtcg ccccgggcgg aaacgctatt gaagcaacgg cagaattcaa
      841 taaacgcttc acggaggata aacttcctgc gggtgttgtt atcgcgcagg gtggcgagac
      901 cgaggacgtg cagaagtcct tcttcgaact catcatcgcg ctctttgcag gagccgcact
      961 catgctttct attctcgtgc ttgagttcaa ctcattccgt cactcgttct acctgcttct
     1021 catcatcccg ctctccctta tcggcgtaca cgccggcctc acccttatgg gtcagccgct
     1081 ctcgctcacc tcaatgctcg gcgtcatcgc gcttgccggc gtcatcatta accacgccat
     1141 cattctgatg gactctatcg cgcgtattca taaagagcgc agtgaactct cgcttgagga
     1201 agtggtggtt gaggcagccg caacgcgtct acgccctatc cttctcacta cggtagtgac
     1261 cgtcgtgggt atgcttccgc ttgcctttgc gtcgccattc tgggcaccgc tcgccttcgc
     1321 tattatgttc ggtctcgcct tctcacttct cctcacgctc gtgcttatcc cgatgctcta
     1381 ctaccgctgg ccaggaaagg cgacgcgtca gcactacgac atggcagaac aagagccgtc
     1441 atagccgact cgttgtcttc ctttattcct tatcggttct atcgtgcacc cagaactgga
     1501 gcgcgcgctc catacgattt gagcgtttca tctcggttga ggtgctcgtc gtcgcttccg
     1561 tcatgagcgt tgccgctttc atcagcatag gggcagcttc cgtggttaca gtggcatctg
     1621 ccactacctc agggcgcaca tcgtctgcat tcaccgcaat aatcacttct ttaccggtgg
     1681 tctccgtaat gcgacgagct gaggtgagcg cggcgtatgc gcggtatgcg cggcgttcaa
     1741 tggaacgata ctcgtct
//
LOCUS       DAORIN010000109         1660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_24389_length_1660_cov_4.441121, whole genome shotgun sequence.
ACCESSION   DAORIN010000109 DAORIN010000000
VERSION     DAORIN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24389_length_1660_cov_4.441121"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..658)
                     /locus_tag="PK109_03640"
     CDS             complement(101..658)
                     /locus_tag="PK109_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRH24650.1"
                     /translation="MSGHSKWSQIKRQKGVSDAAKSRVFSKFAREIALESKKAAGNMS
                     SPSLAAVIARAKAANMPKDNIERAITKGTSKDSGDLEAVVYECYGPGGVAIIATAVTD
                     NRNRTTQEIKHLLSSNDYALGAVGSAAWAFTKSPDGRYAANEPKMTVTGDDETKLGEL
                     LNLLDEHDDVQEVFTNAEGYENTSE"
     gene            678..1160
                     /locus_tag="PK109_03645"
     CDS             678..1160
                     /locus_tag="PK109_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24651.1"
                     /translation="MSLFEHLRPNQDSRDYVRPAEAPSPFSAEVLERDADTVYVKTRP
                     SGTVITFRLVFIDAATQKESTENQEAFLLSARTAADFKKYGGSTMLVLPHPYFAAQQK
                     EAQEQTEEVQKESGWSVTTPEYLAKQLDIPVKEYVAAQQVAYDTLQKAYEVPADFSGF
                     "
     gene            complement(1157..>1660)
                     /locus_tag="PK109_03650"
     CDS             complement(1157..>1660)
                     /locus_tag="PK109_03650"
                     /inference="COORDINATES: protein motif:HMM:NF037140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HRH24652.1"
                     /translation="SGPLRSRFGGGVYQLDFYSDEDLRAIIKRSAGILGLSVPAEVVK
                     MIAGRSRATPRVANALLKRVRDYAQVHEKELTPEVCDLACQMFGIDPLGLTKDDRRYL
                     ETLQGGFKGGPAGIRALASALHEDPDTVENVYEPYLLRLGFIERSPRGRTLTEKGSRY
                     LRDGVSF"
BASE COUNT          397 a          449 c          426 g          388 t
ORIGIN      
        1 ccctcaagta cggcaatgcc aaggcgatca aaccctgggt ctatcgcaag aatacgcatg
       61 caggtattgt cccacactgc tttccacccc tcctacacaa ctattcgctc gtgttttcgt
      121 agccctcagc gtttgtgaag acttcctgca cgtcgtcatg ctcgtcgaga aggtttaaca
      181 actcaccaag ctttgtctcg tcgtcaccgg taacggtcat ctttggttcg tttgcagcat
      241 agcggccatc aggagatttc gtgaatgccc atgcagcact tccgactgca ccgagcgcgt
      301 agtcgtttga cgataagagg tgctttatct cctgcgtcgt acgattgcgg ttatcggtaa
      361 cggcagtggc aattattgcg acgccgcccg ggccatagca ctcgtataca actgcctcga
      421 gatcacccga gtctttggag gtacctttag tgattgcgcg ttcaatattg tctttgggca
      481 tattcgctgc ctttgcgcga gcgatgaccg ccgcaaggga aggggaggac atatttcctg
      541 ccgccttctt cgactcaagc gctatctctc gagcaaactt tgagaagaca cgcgacttgg
      601 ctgcgtcgct tactcccttc tgtcgcttaa tttgtgacca ttttgagtga ccagacatgg
      661 ttggtattat tctacagatg tcactatttg aacatctccg accaaatcag gactcgcgcg
      721 actacgtacg acctgcggaa gcacctagcc cattctccgc tgaagtactt gagagggatg
      781 cggacacggt gtatgtgaaa acacgcccga gcggcacggt tattacgttt cgtctcgtgt
      841 tcatagacgc cgcgactcag aaagaaagca cagagaacca ggaagccttt ctgctgtctg
      901 cacgaacggc ggcagatttt aaaaagtacg gaggctcgac catgctcgta ttaccgcacc
      961 cctattttgc ggctcagcag aaagaggcac aggagcaaac agaggaagtt cagaaagagt
     1021 ccggatggag tgtcaccaca ccggaatatc ttgcgaaaca gctcgacatt ccggtcaaag
     1081 aatacgtagc tgcccagcag gttgcgtatg acacgcttca gaaagcgtac gaagttcccg
     1141 ctgatttcag cggcttttag aacgaaacac cgtcacgcaa atatcgtgaa cctttttccg
     1201 taagggtacg tccgcgaggg gaacgttcga taaacccaag gcgaaggaga tacggctcgt
     1261 acacattctc taccgtgtct gggtcttcgt ggagcgctga cgcaagtgcg cgtataccag
     1321 cagggcctcc tttaaacccg ccttgaaggg tttcaagata gcggcggtca tccttggtga
     1381 gaccaagcgg atcaatacca aacatctggc aggcaaggtc acacacctca ggtgtgagtt
     1441 ctttctcatg tacctgcgca tagtcacgca cgcgttttag aagtgcgttt gcaacgcgcg
     1501 gagtagcgcg gcttcggcct gcgatcatct tcactacttc agcaggaacc gaaagaccaa
     1561 gtatgcctgc cgaacgtttg atgatggcac gcaggtcttc gtcagaataa aagtcgagct
     1621 gatacacgcc gcctccaaag cgtgagcgaa gcggtcccga
//
LOCUS       DAORIN010000110         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_25005_length_1639_cov_2.752525, whole genome shotgun sequence.
ACCESSION   DAORIN010000110 DAORIN010000000
VERSION     DAORIN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25005_length_1639_cov_2.752525"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(470..751)
                     /locus_tag="PK109_03655"
     CDS             complement(470..751)
                     /locus_tag="PK109_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24653.1"
                     /translation="MKRIGDILPGVAKDTSRKTERGELMRYFMTYLNASRAHAKLPML
                     TMPRMGRILEGIPTKDLYYLKSVCEKAKNFSKKFWWEIDPKKHQKDNPF"
     gene            complement(748..1536)
                     /locus_tag="PK109_03660"
     CDS             complement(748..1536)
                     /locus_tag="PK109_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24654.1"
                     /translation="MEKALSIAKSYWIVVLVVVLLLLSFAGYLAGYRLDQAGITRVGT
                     LVLTDLPDGTAVYLDQARRIYAKDGTAKTTFLPGTHTVIVDAPDYQPWNELFSITAAN
                     DTVIAPVLVPVDVMGTGLTGAEATKAMNLIRTQKLPTKAAPLVVNDGCTLLSVSGPRL
                     IADAATSSCAMLPEYLCAPGTEGCASTLIYTAKDAIRSVSPFPGRDDALVVAAGNLVF
                     VVEVDPREPQFFAPLFKGPNIGAALYTASSTIIGDGKTVVEIPL"
BASE COUNT          393 a          401 c          503 g          342 t
ORIGIN      
        1 gtaataagaa gccgacttcc taagaagccg gcagaaagag gtgagcgagg ggcatcctgc
       61 gggatgctgg gttgggcgga tgccccaagc tctgtgtcga ggcggacgag gggggaaacc
      121 gtcccttcca cgacactagg gtcatgctac gcccgctgag cgtcgggtca atggaggtag
      181 gtgcataact tatataaaag aaaaccgatg ccttgagggg catcgggttt ttcacgggcg
      241 ccgggcggag gcacacatag ggcctaccag gaaatcagca cgaatgattc ctgcgactca
      301 ctgtctcacg aatggagatg gacatcgacg ccagcgcgtt caaataacaa gagggcaatg
      361 agtgcgaggc tgcgaggggc taaattgcct cgcactcacg cttgccgggc gaagaaaggg
      421 acccggctac gcgcatccta gggtgtgctt cgctccacgt caatgccgat taaaacggat
      481 tgtccttctg gtgctttttg ggatctatct cccaccagaa ctttttactg aaattctttg
      541 ctttttcaca cacacttttg aggtaataga ggtctttggt gggaatgcct tccagaatgc
      601 gtcccatacg cggcatggtg agcataggaa gttttgcatg cgcgcgtgac gcattcagat
      661 acgtcatgaa atagcgcatt aattcgccgc gctcggtttt gcggctcgta tctttggcga
      721 cgcccggaag gatgtcgccg atgcgcttca tagaggaatt tcaacgaccg tctttccgtc
      781 accaatgata gttgaggaag cggtgtaaag cgccgcaccg atgttcggtc ctttgaaaag
      841 gggcgcgaag aactgcggct cacgcgggtc aacctcgaca acgaagacga gatttcctgc
      901 agcgaccacg agcgcgtcat cgcgtcccgg gaagggagac accgagcgta tcgcatcttt
      961 agcggtatag atgagagtag aggcgcaacc ttcagtgcct ggagcgcaaa gatactcagg
     1021 gagcatggcg caggaagaag tggcggcgtc agcaatgaga cgtggacccg atactgagag
     1081 aagggtgcag ccgtcgttta ctacgagcgg tgcggccttc gtcggaagct tctgtgtgcg
     1141 tatgagattc attgctttcg tcgcctcagc gccggtgaga cccgtgccca taacatcaac
     1201 agggacgagt actggtgcga tgacggtatc gttagcagca gttatagaga acagctcatt
     1261 ccatggttga taatctggag cgtcgacaat gacggtgtgg gtgccgggaa gaaaggtcgt
     1321 cttcgctgta ccgtccttcg cgtagatgcg gcgtgcctga tcaagataga cggcggtgcc
     1381 gtcaggcaga tcagtgagga cgagggtccc tacgcgcgtg atacctgcct gatcaagacg
     1441 gtagcctgcg aggtagcccg caaaactaag aaggagtagt actacaacga gcactacgat
     1501 ccagtatgac tttgcgatag agagagcttt ttccatgaaa gaagttgagt gattagtaaa
     1561 agagtgagcg gctacccacc ggcggactct atcgacgcgc caatagagcg aaggcgtgcg
     1621 acgaggtctt catacccgc
//
LOCUS       DAORIN010000111         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_26855_length_1576_cov_4.687705, whole genome shotgun sequence.
ACCESSION   DAORIN010000111 DAORIN010000000
VERSION     DAORIN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26855_length_1576_cov_4.687705"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="rpoA"
                     /locus_tag="PK109_03665"
     CDS             complement(<1..842)
                     /gene="rpoA"
                     /locus_tag="PK109_03665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRH24655.1"
                     /translation="MEYQITLPSKPRVVSEEGLQGVYEIDSLYPGYGHTLGNSLRRII
                     LSSLPGAAITSVKIEDVAHEFATIDGVRESVMELLLNLRRVHFVLHGDEGQTVKLIAK
                     GPGKITAADFTLPTQVTISNPEAYLCEISGKAGFELEATIERGLGYIAREQLTKEKVD
                     IGTIALDATFTPIRRVNYEVENMRVGDRTDFNRLRILIETNGTVTPREALEKSIEIMI
                     HQLKSVIGFQEEVETASEVSGLIGGTPTVGKDPSKLKIDELGISTRTASALADGGVKS
                     VAGL"
     gene            complement(872..1486)
                     /gene="rpsD"
                     /locus_tag="PK109_03670"
     CDS             complement(872..1486)
                     /gene="rpsD"
                     /locus_tag="PK109_03670"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRH24656.1"
                     /translation="MKTLSKYKIAKRLGATVFEKTQTQKFALAAERAAKNKKRGRGRG
                     ASEFGKQLLEKQKVRFTYGLSERQFSNYIKEAMLAKSMTPAAMLHQLLEMRLDNIAYR
                     IGLAPTRRAARQMVSHGHLTVNGTKTTIPSRAIRIGDRIAIREGSKEAALFAGFAEKF
                     MDRPLPSWLSWNPKAMEGGVSTLPTKEAADPAGDLVAVLSFYSR"
     gene            complement(1496..>1576)
                     /gene="rpsK"
                     /locus_tag="PK109_03675"
     CDS             complement(1496..>1576)
                     /gene="rpsK"
                     /locus_tag="PK109_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017433998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRH24657.1"
                     /translation="GIAIRAIKDMTPIPHNGPRPPKPRRV"
BASE COUNT          359 a          406 c          454 g          357 t
ORIGIN      
        1 ataaggccag caacgctctt cacgccgcca tcagccaatg ctgacgcggt gcgcgttgag
       61 atgccgagct catcaatctt gagctttgat gggtccttac caacggtagg agtgccaccg
      121 atgaggccag atacctcaga agcggtctct acttcctcct ggaagccgat aacggacttc
      181 aactggtgga tcatgatctc aatagacttc tcaagtgcct cgcgtggcgt aacggtgccg
      241 ttggtttcaa taagaatgcg gaggcggttg aagtcggtgc gatcaccaac gcgcatgttc
      301 tctacttcgt agttcacgcg gcgaattggg gtgaaggtgg catcaagcgc aatagtgccg
      361 atgtctacct tttccttggt gagctgctca cgagcaatgt agccgaggcc acgctcaatc
      421 gttgcttcaa gttcaaagcc tgccttacca gaaatctcgc agaggtaggc ctctgggttt
      481 gagatggtga cctgggtagg gagcgtaaag tcagcggcag taattttgcc cggacccttt
      541 gcaataagct ttacggtctg accttcgtct ccgtgaagca caaagtgtac gcggcggagg
      601 ttcaagagaa gctccatgac ggactcacgt acgccgtcga tagttgcaaa ctcatgcgcg
      661 acatcctcaa tcttgactga ggtgatagca gcgccgggga gcgatgaaag gataatgcgg
      721 cggaggctgt tcccgagggt atgaccgtaa cctggataga gtgaatctat ctcataaacg
      781 ccctggagtc cctcttcaga gacaacgcgg ggcttagacg gaagagtgat ttggtattcc
      841 ataacgcaat cgtagttacc tagtttataa attaacggct atagaacgaa agcacggcaa
      901 ccaagtcgcc agccggatca gccgcctcct tggtaggaag cgttgaaacg ccgccctcca
      961 tcgctttcgg attccacgaa agccacgaag gaagcggacg gtccatgaac ttctccgcaa
     1021 accctgcgaa gagtgctgct tccttcgagc cctcgcgtat agcgatgcga tcgccgatgc
     1081 ggatagcacg ggaaggaata gtcgtcttcg taccgtttac ggtgaggtga ccatgagaaa
     1141 ccatctggcg agccgcacgg cgcgttggag cgagtccaat gcggtacgcg atgttatcaa
     1201 ggcgcatttc aagcaactgg tggagcatcg ctgcaggagt catggacttt gcgagcattg
     1261 cttccttgat gtagttagag aactggcgct cagaaaggcc gtacgtgaag cgtaccttct
     1321 gcttctcgag aagctgcttg ccgaattcag acgcaccgcg tccgcggcca cgcttcttgt
     1381 tcttcgcagc gcgttcagcc gcaagtgcaa acttctgcgt ctgcgtcttc tcgaagacgg
     1441 ttgcgccgag gcgctttgcg atcttgtact tggatagagt cttcatatac gaggattaga
     1501 cgcgacgagg cttaggcgga cgtgggccgt tgtgaggaat cggcgtcatg tccttaatag
     1561 cgcggatagc gatgcc
//
LOCUS       DAORIN010000112         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_26977_length_1572_cov_5.702703, whole genome shotgun sequence.
ACCESSION   DAORIN010000112 DAORIN010000000
VERSION     DAORIN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26977_length_1572_cov_5.702703"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PK109_03680"
     CDS             complement(<1..1189)
                     /locus_tag="PK109_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRH24658.1"
                     /translation="MKKKKAPARPKAKAVSRTKKKVVKKAAKKHPVKKAVKKVVAKKK
                     MKAVVRAAVKGAKAKAAGERGNKAEMLIQKGKERGYITYSEILKEFPHIEDNISFLDE
                     LYERFSTAGIDILEGGMLEDATDQYLATRNIKGRESASYDSIQIYLREIGQYPLLTAS
                     EERELAKRIDAGDDEARNILARSNLRLVVSIAKKYVGRSPDLTLLDLIQEGNLGLFKA
                     VDKFDWKKGFKFSTYATWWIRQAITRALADQSRTIRIPVHMVETIAKYKQVSRRLAQT
                     LGRDPQPEEVAVEMGIEVDKVYQIEKINQDTLSLENPIGSDDDEKSTLGDFIADDKIP
                     SPIQESSERILTEQVRDILDDLSPKERKILEMRHGLLDGIYHTLEEVGKEFGVTRERI
                     RQIE"
     gene            complement(1229..>1572)
                     /locus_tag="PK109_03685"
     CDS             complement(1229..>1572)
                     /locus_tag="PK109_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24659.1"
                     /translation="ARGLLILAAIDAYAGTPLAKRLSDEYARIIGTPPADHAIPERAL
                     FEAGLLFGETPSENAGDDLVRAFERTVLTEKLRDATARLRHAESAKDDEAMRRALTDC
                     KEISARLATLG"
BASE COUNT          336 a          416 c          411 g          409 t
ORIGIN      
        1 cttcaatctg gcgaatgcgc tcacgcgtca ctccgaactc cttccctact tcttccaagg
       61 tgtgatagat accgtcaaga agaccgtggc gcatctcaag aatcttacgc tccttcggag
      121 aaagatcgtc gagaatgtcc cggacctgtt cggtcagaat acgttctgaa gactcctgaa
      181 taggcgacgg aatcttatcg tccgcgatga agtcgccaag tgtggatttc tcatcatcgt
      241 ccgaaccaat tgggttctca agagaaagag tatcctgatt aatcttttca atctgataca
      301 ccttatctac ctcaataccc atctctacgg ccacctcttc aggctgtggg tcgcgaccaa
      361 gggtctgagc gagacgacgg gagacctgct tgtacttcgc aatggtttcc accatgtgca
      421 ccggaatacg aatggtgcgg gactggtcag caagtgcgcg cgtaatagcc tggcgaatcc
      481 accaggtggc gtaggtggag aacttgaagc ccttcttcca gtcgaattta tcaacggcct
      541 tgaagagacc gaggttccct tcctgaatga ggtcgagaag cgtgagatca ggagagcgtc
      601 ctacgtactt cttggcgata gacactacga gacgaaggtt gcttcttgca agaatgttgc
      661 gtgcctcatc atcccccgca tcaatgcgct tagcgagttc gcgttcttct gacgcggtga
      721 gaagcggata ctgaccaatc tcccgcaggt aaatctgaat ggagtcatag gaagctgact
      781 cgcgcccttt gatgttgcgg gtcgcaagat actggtcagt ggcgtcctca agcatgccgc
      841 cttcaagaat gtcgatgcct gcggttgaga agcgttcata gagctcatca aggaaggaga
      901 tgttgtcttc gatgtgcggg aactccttca agatttcgct gtacgtaatg tagccgcgct
      961 ccttaccctt ttggataagc atctctgcct tgttaccccg ctcaccggca gccttcgcct
     1021 ttgctccctt tacggcagcg cgcacgactg ccttcatctt ctttttagcg accactttct
     1081 ttaccgcctt tttaacagga tgtttctttg cggccttttt gactaccttt ttcttagtac
     1141 gagaaactgc tttcgccttc gggcgcgcgg gagctttctt ttttttcata ggttttgcca
     1201 tggtgaaggt ggttaagtga aaaatgcgtt aaccgagtgt cgctagtcgg gctgatattt
     1261 ctttgcagtc cgtgagtgct ctacgcatcg cctcatcgtc ttttgcggac tcagcatgcc
     1321 gcaggcgcgc cgttgcatcc cgtagttttt cggtcagcac ggttcgttca aatgcgcgca
     1381 caagatcatc ccctgcattc tcagaaggcg tctctccgaa gagaagaccg gcctcgaaaa
     1441 gggcgcgctc tgggatagca tgatccgcag gaggcgtacc gataattcga gcgtactcgt
     1501 ccgagaggcg ttttgcaagg ggggttcccg cataagcgtc gatagccgca agtatgagga
     1561 ggccacgggc ag
//
LOCUS       DAORIN010000113         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_26982_length_1572_cov_4.597231, whole genome shotgun sequence.
ACCESSION   DAORIN010000113 DAORIN010000000
VERSION     DAORIN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26982_length_1572_cov_4.597231"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PK109_03690"
     CDS             complement(<1..180)
                     /locus_tag="PK109_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase subunit L"
                     /protein_id="HRH24660.1"
                     /translation="MTKPKKILLLGSGGLRIGQAGEFDYSGSQAIKAFKEEGIAVVLM
                     NPNIATVQTDKGLADE"
     gene            complement(177..1226)
                     /gene="carA"
                     /locus_tag="PK109_03695"
     CDS             complement(177..1226)
                     /gene="carA"
                     /locus_tag="PK109_03695"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRH24661.1"
                     /translation="MKLYLEDGSVFEGESFGAPVSATGEAVFTTGMVGYVESLSDPSY
                     TGQILVCTYPLVGNYGVPDEKFWESKKIHAAGLVVSEYSEAYSHHSAKESLSAWLKRN
                     KVPAITGVDTRAITKRLREEGVMLGQLSFTGKKGKFVDPNTENLVAKVSTQKKTITGK
                     GDTTIVLVDCGAKENIARFLERPGVRVIRVPWDYDFTTEAYDGVVISNGPGDPSVCGA
                     AVANIKKAFAKDTPMLGICLGTQLMAMAAGASTYKLKYGHRSQNQPCTIQTGTKRTYI
                     TSQNHGYAIDPKTLPEDWKVWFTNANDGSVEGIRHTTKKWRSVQFHPEAAPGPTDTQW
                     LFEEFFADVAKAKKI"
BASE COUNT          339 a          430 c          399 g          404 t
ORIGIN      
        1 ctcgtcagcg agtcctttgt cggtctgtac cgtcgcgatg ttcgggttca taagcacaac
       61 ggcaattccc tcttctttga acgcctttat cgcttgagag ccggaatagt cgaactcacc
      121 ggcctggccg atacggagcc ctcctgaacc gagcaacaaa attttcttag gcttggtcat
      181 atcttttttg ctttcgcgac gtcggcgaag aattcttcaa agagccattg ggtgtcggtg
      241 ggcccgggag cagcttctgg gtggaactgt actgagcgcc atttcttcgt ggtgtgacga
      301 ataccttcca cgctgccgtc attggcgttg gtgaaccaca ccttccagtc ctcagggagt
      361 gtttttggat cgatggcgta tccatgattc tgcgaggtga tgtaggtgcg cttagtgccg
      421 gtctgtatcg tgcaaggctg attctgtgag cgatgaccgt atttgagttt gtaggtcgac
      481 gctcccgctg ccatcgccat gagctgcgtg ccaagacaaa taccaagcat tggtgtgtcc
      541 ttagcaaatg cctttttgat atttgctacg gcggcaccac acacactcgg gtccccaggg
      601 ccgttactaa taacgacgcc atcgtatgct tcggtggtga agtcatagtc ccacgggacg
      661 cgtatgacgc gaacgccagg gcgctccagg aagcgggcga tgttttcttt tgcgccacaa
      721 tcaacgagga cgatagtggt gtcccctttg cctgttatgg ttttcttttg tgttgaaacc
      781 ttagccacaa ggttctctgt atttgggtca acaaattttc ctttcttacc cgtgaacgaa
      841 agctgtccaa gcatcacacc ttcttcgcgc aagcgcttcg ttatcgcccg cgtgtccact
      901 cccgttatcg ctggcacctt attgcgcttc aaccacgcag acagcgactc tttcgctgag
      961 tggtgcgaat aggcttctga gtattccgag accacaagac cggcagcgtg aatctttttt
     1021 gattcccaga acttctcatc cggtacacca tagtttccta caagcggata ggtgcacacg
     1081 agaatttgac cggtgtagga ggggtcagac agactttcta cgtagccgac catgccggtg
     1141 gtgaacactg cctcaccagt agcagacacg ggagcaccaa aactttctcc ttcgaacacc
     1201 gaaccgtctt cgagatagag cttcatagat aaaaagaaac ctcccgtggg gaggtttctt
     1261 ctataacgct cgaggaggtc attaaagccc tagtatagag ggcgtagcgc atccgtcaaa
     1321 catactgtgg atagcggtac tcacgcatga gagctcggcg catactagtg ccaagctgca
     1381 atccggcagt gagcaccctg ggagattcct catgcgtgcc tttgtatgtg cccttgccct
     1441 acttctggca cctgcttcag tgcaggcgca gaccactgac gtgctcccgc acttcagcca
     1501 aacctatcaa agtatcgtct gcaacaacga agcagcggcc attcatcttg ggaactacta
     1561 ctttgccccc at
//
LOCUS       DAORIN010000114         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_27081_length_1569_cov_4.019815, whole genome shotgun sequence.
ACCESSION   DAORIN010000114 DAORIN010000000
VERSION     DAORIN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27081_length_1569_cov_4.019815"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PK109_03700"
     CDS             <1..670
                     /locus_tag="PK109_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24662.1"
                     /translation="ELEPFRKTDYLYAEEKRVAEERLEAQYKKRTNAATVAIIRPAAI
                     TGPRGRFMRIRFGLQSALSGSLKGQKSFLYDLISLMVSWVPVTPKWLRQYVHEDDIVA
                     VTLRLAFDEVPVKYEAFNIAPPGPAVFGADMARAVGKRTVNMPPWLIRIAFWIMWHIS
                     RGKIPTAEGSWKGYSYPIAVDGSKATRLLGIKYSYESYDAFYYTNGTYESEVPEPLRR
                     HKDA"
     gene            678..>1569
                     /locus_tag="PK109_03705"
     CDS             678..>1569
                     /locus_tag="PK109_03705"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRH24663.1"
                     /translation="MKKVVVTGGAGFIGSHLCEALQAAGYDTHAVDNLANGHREDVPE
                     GVTFHEVDVLDTEALLPIVENAHAVFHLAALPRVPFSFDHPVESHRANIDGTFSVLMA
                     ARDGKVRRVIYAASSSSYGDQPTLPFTEDMQPAPMSLYSFQKLVGEEYARLFSSNYGL
                     KTVSLRFFSVYGPKMRPDGGYALAIPKFLKCIKDGVKCPVTGDGTQTRDFTHVRDTVR
                     ALMLAMESEKVGAGEVINVAAGRNVSVNALVSLMGGTIEYIPKRPADATDTFGSIEKA
                     KELLGWSPEVRLEDGITELKV"
BASE COUNT          372 a          401 c          427 g          369 t
ORIGIN      
        1 agaactcgag ccgttccgca aaactgatta tttgtatgca gaagagaagc gtgtggctga
       61 agagcgactc gaggctcagt acaaaaagcg cacgaacgcc gcaaccgttg ccatcattcg
      121 tcctgccgct atcaccggtc ctcgtggccg ctttatgcgt attcgttttg gactccagtc
      181 agcactctct ggaagtttga agggtcagaa gtcgtttctg tatgacctca tctcgctcat
      241 ggtgtcgtgg gtcccggtaa cgcccaagtg gcttcgccag tatgtccacg aagatgacat
      301 tgtcgctgtg acgcttcgtc ttgcgtttga tgaggtgccc gtcaaatacg aggcgttcaa
      361 tatcgcgcca ccagggcccg cagtgtttgg cgctgacatg gcgcgagccg tggggaagcg
      421 caccgtgaat atgccgccct ggctcattcg tatcgcgttc tggatcatgt ggcacatctc
      481 tcgcggaaag attcctacgg cggaagggag ttggaaaggg tactcgtacc cgatagcggt
      541 agatggctct aaagcaacgc gcttacttgg tatcaagtat tcgtacgaaa gctatgacgc
      601 gttctactac accaacggta cgtacgagtc tgaagtccca gaaccgcttc gccgtcacaa
      661 ggatgcataa tgaggatatg aaaaaagtag tcgttactgg cggcgcggga tttattggtt
      721 cacatctctg tgaagcactg caggctgcag gatacgacac acatgcagtc gacaatcttg
      781 cgaacggaca ccgcgaagat gttcctgaag gagtcacgtt ccatgaagta gatgtgcttg
      841 ataccgaagc gcttctgccg atagtagaga acgcgcacgc cgtcttccat cttgcagcac
      901 ttccgcgcgt gccgttctcg tttgatcatc cggtagagag tcaccgagca aatatcgatg
      961 gcaccttctc agtgcttatg gccgctcgcg acggtaaggt acgtcgcgtt atttatgccg
     1021 cttcaagttc ttcgtatggc gaccagccca cacttccatt taccgaagac atgcagccag
     1081 caccgatgag tctctactcc tttcaaaagt tagtcggtga agaatatgca cgactctttt
     1141 caagtaacta tggactcaag accgtatcgt tacggttctt cagtgtgtat ggcccgaaga
     1201 tgcgccccga tggcggctac gcgttagcaa tacccaaatt cttgaagtgc atcaaagacg
     1261 gcgtgaagtg tccggtcacc ggtgacggaa cacagactcg tgactttacg catgtacggg
     1321 acaccgtgcg cgcactcatg cttgctatgg aaagcgagaa ggtgggggca ggggaagtca
     1381 ttaatgttgc cgcagggcgg aacgtctcgg tgaacgcgct tgtttcgctt atgggcggca
     1441 ctatcgaata cataccaaag cgtcccgctg acgcaaccga taccttcggc agtatcgaaa
     1501 aagcaaaaga acttttgggc tggagccctg aagttcgtct tgaagacggc attactgaat
     1561 taaaagtag
//
LOCUS       DAORIN010000115         1555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_27502_length_1555_cov_5.510000, whole genome shotgun sequence.
ACCESSION   DAORIN010000115 DAORIN010000000
VERSION     DAORIN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27502_length_1555_cov_5.510000"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1172
                     /locus_tag="PK109_03710"
     CDS             48..1172
                     /locus_tag="PK109_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRH24664.1"
                     /translation="MNASPSATRILFVITKANWGGAQRYVYDLAIAAKEKGMAVTVAF
                     GTDGLLRTKLEAAGVRTTSLSTLTREVSFRDELETFHALRALIKEERPDIIHVNSSKA
                     GLGLLAARLEKVPHIIFTAHGWAFNEKRPWWQKVLLRLIYLATVYLAHTTICVSDAVR
                     RDLGFVPGKSLLVIQHGIDAPAFLPKEEARKKLFIGTVEGRWIGMTAELHPTKRVEDA
                     IDAVAELSRGYPDLHLVVMGEGELKEWLIERVKHYGLEKRIHLPGFIIDAPEYLKAFD
                     FFLMPSRTEALGLALVEAGYAGLPVVAARAGGIPEVVTHKKTGLLVPRENPHSLARAI
                     RTLLEHPEDAALYGAALESSTRERFSRERMIKETFAVYGI"
BASE COUNT          341 a          419 c          409 g          386 t
ORIGIN      
        1 ggaggggcgc tttccgatgt tatatcatag gaagaacgtt acgtcccatg aacgcctccc
       61 cgagcgccac gcgcatactc tttgttatta ccaaagcgaa ctggggcggc gcccagcgct
      121 acgtctacga tcttgcgata gcggccaaag aaaaaggcat ggccgtgacc gttgcgtttg
      181 ggaccgatgg actactccgc accaaacttg aagcagcggg agtgcgtacc acttccctct
      241 cgacccttac acgcgaagtg agtttccgtg acgagcttga gaccttccac gcactccgtg
      301 ctcttataaa ggaagagcgt cctgacatca tccatgtaaa cagctcgaag gcagggcttg
      361 gtcttcttgc cgctcgtctt gagaaagtac ctcatatcat ttttactgcc cacggctggg
      421 cctttaatga gaagcgtccc tggtggcaga aggtactcct tcgcctcatt taccttgcca
      481 ctgtctattt ggcccacacc actatctgcg tatcggatgc ggtacgccgc gaccttgggt
      541 ttgtgccggg taaatcactc ttggtgattc agcacggtat cgatgcacct gcgttccttc
      601 cgaaggagga ggcgagaaag aaacttttca ttggtacggt agaaggtcga tggattggta
      661 tgaccgcaga acttcacccc accaagcgcg ttgaggacgc tatcgacgcc gttgcagaac
      721 tctcccgcgg ctaccctgac ctccacttag tggttatggg cgaaggtgaa ttgaaggagt
      781 ggctcattga acgcgtgaag cattatggcc ttgagaaacg cattcacctt cctgggttta
      841 ttatcgatgc gcctgaatat ttgaaagcgt ttgatttctt cctgatgcca agccgtactg
      901 aggcacttgg tcttgccttg gttgaagcag ggtatgccgg acttccggtg gttgccgcac
      961 gcgctggcgg tatccctgaa gtggtgaccc ataagaagac gggtctactg gtgccgcgcg
     1021 aaaacccgca ctcgcttgcg cgcgctattc gaaccctgct tgaacaccct gaggacgccg
     1081 ctctctacgg ggccgcgctt gaatcctcaa cccgagagcg attctccaga gagcgaatga
     1141 taaaggaaac gtttgctgtc tacggtatat aaaacgaaac cggttgcttg aggcgcaacc
     1201 ggcgaaagcc tttcgtacta cgttggagct tcttgctcaa atacggtgat gaattcacgc
     1261 gctttgatga ttgagaacgc gtaaaatgcg ttcacggtca tttgttctgg tagcgcatca
     1321 aacgttctct gatttctcgt ttgcgcgcac cacctcgagt ttccttctcc ggtactgaga
     1381 ccgaaaagag cttccccttc ccgcaactgc tggtacagca gcggatattc gttgccttca
     1441 agtttccggc catgcttcag aagcgcatga acgacaggtg tttctagggg cgaataagcc
     1501 attcactatc tttcttttct gtcgtgattg ggtaggcgca tcctaaacga agcac
//
LOCUS       DAORIN010000116         1553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_27572_length_1553_cov_4.087450, whole genome shotgun sequence.
ACCESSION   DAORIN010000116 DAORIN010000000
VERSION     DAORIN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27572_length_1553_cov_4.087450"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PK109_03715"
     CDS             complement(<1..498)
                     /locus_tag="PK109_03715"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRH24665.1"
                     /translation="MTYLDSIILGIVEGLTEFLPVSSTGHLILTSSLLGLPDTEFLKS
                     FEIAIQLGAIAAVVLIYWRSFLDVAVLKRLLVAFIPTAVVGYILYKFAKSYLLGNEGV
                     VVAALFIGGIVMLLIEYAVRNREQNEGAVTDITYKQSFLIGLAQAVAIIPGVSRSGAS
                     VIGGLM"
     gene            533..1036
                     /locus_tag="PK109_03720"
     CDS             533..1036
                     /locus_tag="PK109_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRH24666.1"
                     /translation="MKIYVTGVAGSGKSSIVRALAARGVTAIDMDQGLCHWENRHTGG
                     MAGWEPGRSEEWYAEHGWMCDVPELSSLLAIHDDIVVVGISSNQEEYMKFFDKVFVLH
                     SKPETIIARINARTDSEYGKHPAEQKRLLAWQKSFEEEMAAKGAIPLDGERPIEEVVE
                     DIVKELQ"
     gene            1339..>1553
                     /locus_tag="PK109_03725"
     CDS             1339..>1553
                     /locus_tag="PK109_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24667.1"
                     /translation="MSEIKVDIAGRRLGVIINAASGGSDASTRERMAAVLAEVGITPT
                     KYWEVTGKELEGALKEAQAYDVLISLG"
BASE COUNT          410 a          356 c          460 g          327 t
ORIGIN      
        1 cataaggccg cctatcactg aggcgccgct tctcgacacg ccgggaatta tggcgactgc
       61 ctgcgcaaga ccaataagga acgattgctt atacgtaata tcggttactg ctccttcatt
      121 ctgttcacga ttgcgtaccg catattcaat caacagcatc acgatgccac cgataaagag
      181 tgcggcaact accactccct cgtttccgag aagataactc ttcgcgaatt tatagagaat
      241 atagccaacg accgccgttg ggataaacgc tacaagcagg cgtttcagaa cggcaacatc
      301 gagaaacgag cgccagtaga tcagaaccac ggcggcgata gcaccgagct gtatcgctat
      361 ctcaaaggac ttaagaaact cagtgtccgg gagtccaaga agggaactgg tgagtatgag
      421 gtggccggta gaggatacgg ggaggaattc ggtaagccct tcaacaatac cgaggatgat
      481 gctgtcgagg taggtcatta cggcagagta taacgcatgc tactgtgcgg gagtgaaaat
      541 atacgtcacc ggcgtagcgg ggagcggaaa gtcgagcatc gtgcgggcac ttgcggcgcg
      601 cggcgttacc gccatagaca tggaccaagg cctttgccat tgggagaacc ggcacacggg
      661 cggcatggcg ggttgggagc caggacggag cgaggagtgg tatgccgagc acggctggat
      721 gtgtgacgtg ccggaactaa gttcgctcct cgctattcat gacgatattg ttgtcgtcgg
      781 tatctcctca aaccaggagg agtatatgaa gtttttcgat aaggtattcg tgctccattc
      841 aaaacctgaa actatcatcg cgcgcatcaa tgcaagaacg gatagtgagt atggaaagca
      901 tccggcagaa caaaagcggt tgcttgcatg gcagaagagt tttgaagagg agatggcggc
      961 caaaggagcc attccactag atggagagcg tccgatagag gaagtggtag aggatattgt
     1021 gaaagagttg cagtaacaaa cggcggccac aggggccgcc ggttgctgtt cttatactcg
     1081 gaacgattta catcgcttct ccctcggaag cgggagcagc ggtcatcgcg gtgcagccgc
     1141 agtcgcaggt gccgtcagcc atatgctcat gtccgcaggt atcgcaggtg ttcatgatgt
     1201 gggtacgtta atggtagtcg cacgttccgg aagtatatca atctgttttc tcccgcacag
     1261 agagttttac acttctctct atgaagtctt gatgagcgcc ataaatgacg aatcagtaaa
     1321 agggtatatc ctaaggatat gagtgagatt aaggtagata tcgcagggcg ccggctcggc
     1381 gtcatcatca atgcggcaag cggcgggagc gatgcgtcga ctcgtgaacg catggcggca
     1441 gtattagctg aagtgggtat tacccccaca aagtactggg aagtaacggg gaaggaactc
     1501 gaaggagcgc tcaaggaagc gcaggcgtat gacgtactca tatccttagg ata
//
LOCUS       DAORIN010000117         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_28032_length_1541_cov_5.191790, whole genome shotgun sequence.
ACCESSION   DAORIN010000117 DAORIN010000000
VERSION     DAORIN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28032_length_1541_cov_5.191790"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..329
                     /locus_tag="PK109_03730"
     CDS             174..329
                     /locus_tag="PK109_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24668.1"
                     /translation="MENIKIEQHTALGGAWFAGWLFTIGFLHLGFWKGFLALVLWPYF
                     LGLVFAL"
     gene            347..856
                     /locus_tag="PK109_03735"
     CDS             347..856
                     /locus_tag="PK109_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24669.1"
                     /translation="MATKKQTAAAKKNIRSAQKAWKGMTKRQRALAQPQGRSRTKPGV
                     GGAGKFYHIEIRPKGQFTSFRTQDVGKKGGLERVAGRRSSGSWATATWLVSKDHARMV
                     GKKLVITNAKEKASLAKAIRGPITHVKGDVFHAHPAKNVPEAAKPTPAMKRAQAKNIK
                     KAQAARRAR"
     gene            complement(853..1410)
                     /locus_tag="PK109_03740"
     CDS             complement(853..1410)
                     /locus_tag="PK109_03740"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRH24670.1"
                     /translation="MVLKVATTSEEGGEVWYSVAFDDWIRYPSRIRDAWYIRSDSARV
                     FESIASEELPETGPASTTKHIIVDRSEQTLYAYDGDTLAMTLKISTGLALTPTPRGTF
                     TIYRKTPSRYMQGPLPGISDQYYDLPGVPWNLYFTHEGAVIHGAYWHDHFGTPWSHGC
                     VNVSLSDARTLYEWADVGTEVTVQD"
BASE COUNT          386 a          391 c          405 g          359 t
ORIGIN      
        1 gagaaaacgg agcgctatgt aaactgcgca aataatacgt gccacctgca ctttttagcg
       61 tgtgacgctt gtcaggaagg tggagtgccg ttctgcagct ctgaatgcgg cgttggttcc
      121 taatacgcac cccggcaaaa aatacctgtt gagcagcgct atactttata ggtatggaaa
      181 atataaaaat agaacaacat actgcgctcg gcggagcatg gtttgcgggc tggctcttca
      241 ctattgggtt tttacatctc gggttctgga agggctttct tgcactcgtc ctctggccgt
      301 atttcctagg ccttgtgttc gcgttgtaag ctcagatctt atttttatgg ctactaaaaa
      361 acaaaccgca gcagcgaaga aaaacattcg ttccgctcag aaagcatgga aaggtatgac
      421 caagcgccaa cgcgcactcg cgcagccgca aggacgcagc cgtacgaagc ctggagtcgg
      481 aggcgccgga aagttctatc acattgagat tcgacctaaa ggacaattca cctcatttcg
      541 tacgcaggac gtcggcaaga aaggcggtct tgaacgcgtt gccggtagac gcagcagtgg
      601 cagctgggct accgcgacct ggctcgtatc aaaagaccac gctcgcatgg taggtaagaa
      661 gctcgtaatc actaacgcaa aagaaaaggc atcactcgcc aaagcgattc gtggtccaat
      721 cacgcacgtg aagggagacg tcttccatgc gcaccctgct aagaacgtgc ctgaggcagc
      781 aaagcctact cccgctatga agcgtgcaca ggccaagaat attaagaaag cacaagcagc
      841 acgtcgcgcg cgttaatcct gcaccgttac ttccgttccc acgtccgccc attcatagag
      901 cgttcgtgcg tcactaaggc ttacattcac acatccgtgt gaccagggcg ttccaaaatg
      961 atcgtgccag tacgctccgt gaatgaccgc gccctcgtga gtgaaataga gattccaggg
     1021 aacccccgga aggtcatagt actgatcgct aatacccggt aagggtccct gcatgtagcg
     1081 gctcggggtt ttgcgataga tggtaaaggt accgcgcggg gtaggggtaa gagcgagtcc
     1141 cgtcgatatt tttagtgtca tcgcgagcgt atcaccgtca taggcgtaca gcgtctgttc
     1201 tgaacgatca acaataatat gtttggtggt tgatgctggt ccggtttcag gaagctcttc
     1261 acttgcgata ctttcaaata ctcgcgcgga atcgcttcgt atgtaccatg catcgcgaat
     1321 gcgacttgga tagcgtatcc aatcatcaaa cgcgactgag taccatacct ctccgccttc
     1381 ttcgctcgtt gttgctacct ttaataccat tccggaacga agggtggctg taactgatga
     1441 agaagccgag ggactcatgt acgcatgggc gcaggttcct tcatagtacg catcacagct
     1501 atttatgatt tctataaacg tacttggcgg tggcggcagg g
//
LOCUS       DAORIN010000118         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_28606_length_1524_cov_5.550034, whole genome shotgun sequence.
ACCESSION   DAORIN010000118 DAORIN010000000
VERSION     DAORIN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28606_length_1524_cov_5.550034"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1524)
                     /locus_tag="PK109_03745"
     CDS             complement(<1..>1524)
                     /locus_tag="PK109_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRH24671.1"
                     /translation="TMEELRRIMEARGIHNAFSVLAKLRSPHLEDDFHRFLIAYISQG
                     LPPKGLNEHAPEWKALHMTLMEIQLPEPDSEPDKDERYKHFKELVQSMEQIYAGMYAI
                     DTPFAGEPSYYTLELAVPTDSPHLRFYASVPTRRKDLFEKNVLAVFPNAVIHVQPNDY
                     NIFSQDGFSVGSSARLAYKGPLSLTDYTEFEADPMTSIFNAFAKIQEGGEGAAVQFVI
                     KPAGVHYQKTYRKILKALRNGEHRARAFEISDNLFFELLREFRWMFRPAKKERENYVD
                     EPTIKSVERKQASTIVEANIRIVASSRDEMHAHQMLDELEATFHQFESTTGNRLIFTR
                     PKGGALRRLFRSFSWRLFSHEHLMPLTTAEMTTMYHFPAKGVKGTPHLKQTRFTAAPP
                     PLEMPSTGVLLGTSNYRGQEVPIRLDTLDRARHLYVVGQTGTGKTSFMKTLIDQDIKA
                     GNGVCFIDPHGNDILDVLAAVPPERYEDVIYFDPAHLDRPFGLNLLEYDARFPEQKTF
                     "
BASE COUNT          372 a          389 c          413 g          350 t
ORIGIN      
        1 aaacgtcttt tgttctggga accgggcatc gtattcaaga aggttcaacc caaacggacg
       61 atccaggtgc gctgggtcaa agtaaataac atcttcatag cgctcgggag gaacggcagc
      121 gagcacgtcg agaatgtcgt taccgtgtgg gtcaataaag cacacgccgt tccccgcctt
      181 aatatcctga tcgataagcg ttttcataaa cgacgtctta ccggtaccag tctgtccgac
      241 gacatacaga tggcgcgcac gatcaagcgt atcgagtcgt atcggcactt cctgtccacg
      301 atagttactg gtgccaagga ggacacctgt agacggcatc tcaagaggag gaggagccgc
      361 cgtgaagcgc gtctgcttca agtgtggggt acccttcaca cccttggctg ggaagtgata
      421 catcgtggtc atctcagcgg tagtgagcgg catcaagtgc tcatgggaga acaaacgcca
      481 ggagaagctg cggaagagac gacgaagggc cccgccttta ggacgcgtaa atataaggcg
      541 atttccggta gtgctctcga actgatggaa cgtagcctca agttcatcaa gcatctggtg
      601 ggcatgcatc tcatcgcggg acgacgcgac gatgcggata tttgcttcaa cgattgtgga
      661 cgcttgctta cgctctaccg acttaatagt cggttcgtct acataattct cacgctcttt
      721 cttcgcagga cggaacatcc aacggaattc tcgaagcaac tcaaagaaaa gattatctga
      781 aatctcaaac gcgcgagcac gatgctcacc gtttcgaagc gctttcagta tctttcggta
      841 ggtcttttga tagtgaacac cggcaggctt aataacaaac tgcactgcag cgccttcccc
      901 accttcctga attttcgcga acgcattgaa tattgaggtc atcggatcgg cctcaaactc
      961 cgtgtagtcc gttaaggaaa gcggtccctt gtacgcaagg cgtgcagaag agcctacaga
     1021 gaacccgtct tgcgagaaga tgttgtagtc gtttggctga acgtgaatga cggcgttcgg
     1081 gaataccgca agtacgttct tctcaaacaa gtctttacgg cgcgttggta ctgacgcata
     1141 gaagcgcagg tgcggcgaat cagtcgggac cgcaagctca agcgtgtagt acgacggctc
     1201 gccggcaaac ggcgtatcaa tggcatacat accggcatat atctgctcca tggactgcac
     1261 cagctccttg aagtgcttat accgctcatc tttgtcaggc tcagagtcag gttctggaag
     1321 ctgtatctcc atgagcgtca tatggagcgc cttccactca ggcgcatgtt cattcaatcc
     1381 ctttggaggc agtccttggc tgatataagc gatgaggaag cggtggaagt cgtcctcaag
     1441 gtgcgggctg cggagttttg cgagcacgga gaacgcgtta tgaatgccac gcgcttccat
     1501 aatgcggcga agctcctcca tcgt
//
LOCUS       DAORIN010000119         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_28789_length_1519_cov_4.291667, whole genome shotgun sequence.
ACCESSION   DAORIN010000119 DAORIN010000000
VERSION     DAORIN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28789_length_1519_cov_4.291667"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PK109_03750"
     CDS             <1..374
                     /locus_tag="PK109_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRH24672.1"
                     /translation="PPQRSFLPAIFPVPMISIDEFSKIEVKVGTVLAAERVPETDKLI
                     KCSVDFGEAEGPRTIVSGIALHVPEPETLIGRQLAYVTNLAPRTIKGIESNGMLFAVG
                     SGDTFAFLTPDRPVPPGTGAH"
     gene            419..1501
                     /gene="pilM"
                     /locus_tag="PK109_03755"
     CDS             419..1501
                     /gene="pilM"
                     /locus_tag="PK109_03755"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRH24673.1"
                     /translation="MNALSRRIRVELPPPPYLSLSTVGIDISTSGIKIARLRERAHGL
                     ELADFDELPLPSGTVVSGEIRDPAAVIEGLKTLAKKHHIHTANIGLPESRGYLFEAEV
                     PDGTLDEAKTAVEQRLDEYVPLPASEVAFAVARLPKVGERARVVGAGYSHHVLDDSLA
                     AMDGAGIETRAIESETFALPRALLRKGDEETVLIIDIGKTTTKLLVATNRLPRYVTTL
                     DIGGHALTLAVAKYFGVSEEEAKRVKAERGIVAGVGNEEYLASMLTTVSVIREEIGKR
                     LDYWQTRPETGVAHDPVTRVILVGGNATVLGLPEYLQTTLKVPVELGDVFANFAPRDK
                     WLPPLEYTESLAYGTAIGLALREYVG"
BASE COUNT          331 a          398 c          433 g          357 t
ORIGIN      
        1 atccgccgca gcgctcattc ttaccagcta tctttcccgt acccatgatt agcattgatg
       61 agttttcaaa gattgaagta aaagtgggca ccgtgcttgc tgctgagcgc gtgccagaaa
      121 ctgacaagct catcaagtgc agcgttgatt ttggtgaagc ggaaggaccg cgcacgatag
      181 tgtcaggcat tgcgctccat gtccccgaac cagagaccct cattggtcgg cagctggcgt
      241 atgtgacgaa tctcgcgccc cgcactatta agggaataga aagtaacggc atgttatttg
      301 cggtagggtc gggtgacacc ttcgctttcc ttacgccgga ccgtccggtg cctccgggca
      361 cgggcgcgca ctaacagtcg ctcctccgct tcgtgcgctc aggcggtata cttttgatat
      421 gaacgctctt tcgcgccgca ttcgcgtaga acttccaccg ccaccgtacc tttctctttc
      481 aacggttggt attgatattt cgacgagcgg tataaagatc gcgcgactgc gcgagcgcgc
      541 ccacggcctt gagctggctg actttgatga actcccgctt ccatcaggaa cggtagtctc
      601 aggagagata cgtgatccgg ctgcagttat tgagggactc aagacgcttg cgaagaaaca
      661 ccacatccac acggctaaca tcggtcttcc agaatcgcgc ggctacctct ttgaagcaga
      721 agtgcccgac ggcaccttgg acgaagcaaa gaccgcggtt gaacagcgtc ttgatgagta
      781 tgtgccgctt ccagcttctg aagtagcgtt tgcggtagct cgccttccga aagtagggga
      841 gagggcacgg gtcgtggggg caggctactc gcaccatgtg ctcgatgatt ctttggcggc
      901 gatggatgga gcaggtattg agacgcgtgc cattgaaagt gagacgtttg cgctcccacg
      961 tgccctttta cgtaaaggag acgaagagac cgtgctcatc atcgatatcg gaaagacgac
     1021 caccaaactt ttggttgcta ccaatcgtct cccgcgctac gtcaccacgc ttgatatcgg
     1081 gggccatgca ctcactctcg ctgttgctaa atactttggt gtctctgaag aagaagcaaa
     1141 gcgcgtgaag gcagaacgcg gcattgtcgc tggggtgggg aacgaagagt atcttgcgtc
     1201 gatgttgacg accgtctctg tgatacgcga agagataggg aagcgtcttg attattggca
     1261 gacacgtccg gagacgggag tagcgcatga tccggtcacg cgtgtgattt tggttggagg
     1321 gaacgctacc gtgctcggac ttccggaata tttacagacg acgcttaagg ttcctgttga
     1381 actcggtgat gtgttcgcta actttgcgcc gcgagataaa tggttgccgc cgcttgagta
     1441 cacggagtcc ctcgcatacg gcacggccat tggcttagcg ctacgagaat atgtcggctg
     1501 aactcactaa tcttcttcc
//
LOCUS       DAORIN010000120         1497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_29586_length_1497_cov_4.302358, whole genome shotgun sequence.
ACCESSION   DAORIN010000120 DAORIN010000000
VERSION     DAORIN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29586_length_1497_cov_4.302358"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PK109_03760"
     CDS             <1..1021
                     /locus_tag="PK109_03760"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRH24674.1"
                     /translation="GPYKGGIRFHTSADEDEVEALAAMMAIKCAVVAIPFGGAKGGVN
                     VDAKLLSKKELEALSRAYVRAFAEHLGPDIDIPAPDVNTTPEIMDWMLDEYETIMGTK
                     APALITGKSLARGGSVGRDTATADGAVIVLNALLSDRHMSPDKLTAAVQGAGNAGAQA
                     AHLLSALGMKIVALADSKGTLTDENGINVAAALKAKEVERRLGEFLSSPEAVVETACD
                     LLVPAALEEQVRIDNVDRVTANIILEIANGPVTPEADEKLSARGTVIVPDVLANAGGV
                     TVSYFEWLQNKSGEVWTKEAVHDRLEATMKDAYRDVADFALDRGVSMREAAYALALSR
                     ILDAA"
     gene            1209..>1497
                     /locus_tag="PK109_03765"
     CDS             1209..>1497
                     /locus_tag="PK109_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24675.1"
                     /translation="MPTRAKPRKKHHWSYYALMWFLGLVALAAIGFVVVNWNRQPVMD
                     PAQTAAITWGDACDPMALKATCGGDQVTERNGRRWTVGEGTDDEYNFSVLRN"
BASE COUNT          361 a          388 c          432 g          316 t
ORIGIN      
        1 aggcccgtac aaaggtggta ttcgttttca tacgagcgca gacgaagatg aggtggaagc
       61 gcttgccgcc atgatggcga taaagtgtgc cgtcgttgct atcccgtttg gtggcgctaa
      121 aggcggcgtg aatgtggacg ctaagcttct ttcaaaaaaa gaactcgaag cgctctcacg
      181 tgcgtatgta cgcgcgttcg ccgaacatct tggtcctgac atcgatatcc cagcgcccga
      241 tgtgaacacg acgccagaga ttatggattg gatgctcgat gagtacgaaa ccattatggg
      301 tacgaaagcg cctgcactca ttacgggaaa gtcgcttgct cgagggggaa gcgtaggacg
      361 cgacactgca acggcagacg gcgccgtcat tgtcttgaat gccttgcttt cagataggca
      421 tatgagcccc gacaaactca cagcagcggt gcagggagca ggaaatgcgg gtgcacaggc
      481 tgcccacctc ttgtcagcgc ttggtatgaa gatagtcgcg cttgccgaca gtaaagggac
      541 actcactgac gagaacggca ttaatgtcgc agctgcgctg aaagcaaaag aagtagagcg
      601 ccgccttgga gagttccttt cctcgcctga agcagtggtt gagactgcct gcgatctttt
      661 ggtacctgct gcgcttgaag aacaagtacg cattgataac gtggatcgcg ttacagcaaa
      721 cattattctt gagattgcca acggacccgt cactcctgaa gcggacgaga aactctctgc
      781 tcgcggcacc gttatcgtcc ctgacgtact cgcaaacgcc ggcggggtca ccgtctccta
      841 ttttgaatgg cttcagaaca agtcaggcga ggtgtggacc aaggaagcgg tacatgaccg
      901 tcttgaggca actatgaagg atgcgtacag agatgttgct gattttgcgc ttgatagggg
      961 agtttctatg cgcgaagcgg cgtatgcatt agcactttcg cgtattcttg acgctgcttg
     1021 acgccgcttc gtatttatag aattatcaca tcggacgttc atcgatagag cgtcgtcaat
     1081 gaggagtaca acgaatggcg aacgaccctg cggagacaac ttctccggaa ggcaccacgc
     1141 ctccacccac ggatcagacg gagctggttc ttaccgaacc ggagccagaa gaaaccattc
     1201 aacaacccgt gcctactcgg gccaaaccgc gcaagaagca tcactggagt tactacgcgc
     1261 ttatgtggtt tctcggactc gtcgcgctcg cggccattgg ctttgtggtg gtgaactgga
     1321 atcgacaacc cgtgatggat ccggcgcaaa ccgcggccat cacctggggt gatgcctgtg
     1381 atccaatggc actcaaagca acgtgcggcg gcgaccaagt taccgaacgt aacggacgtc
     1441 gctggacggt gggagaaggc accgacgacg agtacaactt cagcgtgctc cggaacg
//
LOCUS       DAORIN010000121         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_30033_length_1485_cov_2.588811, whole genome shotgun sequence.
ACCESSION   DAORIN010000121 DAORIN010000000
VERSION     DAORIN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30033_length_1485_cov_2.588811"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PK109_03770"
     CDS             complement(<1..271)
                     /locus_tag="PK109_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRH24676.1"
                     /translation="MSALLWIGIGVGAFILIVLVSMYNGFVRLRNEVRNSLAQIDVQL
                     KRRNDLIPNLVETVKGYAKHEKQLFENVTQARAAMLGADTNAEKAK"
     gene            complement(814..1197)
                     /locus_tag="PK109_03775"
     CDS             complement(814..1197)
                     /locus_tag="PK109_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24677.1"
                     /translation="MSNSPHYCVALSVDHAISHVSYKIPERTEVVRSFSIPQKDGIDD
                     VVAVNAAFEREVLALQSEFGFGNPYNDGLVTIALTSFTRNGQQVLDLSKPRVVFTYNP
                     LADFLASIAREELVKAGEPVPAWLK"
     gene            complement(1197..>1485)
                     /locus_tag="PK109_03780"
     CDS             complement(1197..>1485)
                     /locus_tag="PK109_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24678.1"
                     /translation="AARWILTTEPKRLEVRSPIRGFYSSTPETHAPSHVYHTVDALLN
                     SPDIAQNAFLVIPEVSFDGVLMPLVIENFQRSPRAIIRLSQSLPAPSNEEE"
BASE COUNT          376 a          328 c          419 g          362 t
ORIGIN      
        1 cctttgcttt ctcagcgttg gtgtccgcgc caagcatcgc tgcgcgcgct tgcgtcacgt
       61 tctcaaaaag ctgtttctcg tgcttggcgt agcccttgac ggtctcaacg agatttggaa
      121 tgaggtcgtt tcggcgtttg agttgcacgt cgatctgcgc gagactgttg cgaacttcgt
      181 tgcgcaggcg cacgaagccg ttgtacatcg agacgaggac gatgagaatg aaggcgccga
      241 cgccgatacc aatccagagg agggctgaca tagggcgtaa cgagcgaagg tgccttttat
      301 agttagtgga cgctgcggaa agactcgtgg catctagccg ccaaggagta gagacgatgg
      361 agtccacgga tacgtagaat caaggaatgt gaaggtatcg atcggttctc ctgtgggtcg
      421 ttgattcggc ggcaaaaggt cgtcgagatt tcgtatgcca aacccgaatc gcgtttcgta
      481 tgcaaactcc gtaagatacg ctgatcgctg gataacagtg ggccaaatgc gctggaaagc
      541 agcatctgaa gagaacgagt cacgagaaag tgtttcaccc gctcgcaaga cgagcacttt
      601 agcgtgaata ccgcgcactc gggcggctgc agcgtgatga tgtccatcta ttgcaagata
      661 cgctccgcga cccatgtact tgacaacggt gggtggttca cgtgcaaatg cttcgggcga
      721 aagtgcacag agtttttcta aataacccca tgagatataa ttctgactgg gagctattcg
      781 ttcaggaggg atgtgaagaa gtttgaccaa ggcttatttc aaccaggccg gtacgggctc
      841 gcctgccttc acgagttctt ctcttgcaat actagcgagg aagtcagcaa gtggattgta
      901 agtgaagacc actcgcggtt tagaaaggtc cagaacttgt tgaccatttc gtgtaaatga
      961 ggtaagtgct atcgttacca atccgtcatt gtaaggattg ccaaaaccga attcgctttg
     1021 tagagcaaga acttcccgtt caaaagcagc atttaccgca acaacgtcat caataccgtc
     1081 tttctgagga attgagaatg aacgtacaac ctctgttcgt tccggaattt tatacgatac
     1141 atgagaaata gcgtggtcga cggagagtgc aacgcagtag tggggagagt tagacattat
     1201 tcttcctcat ttgatggcgc gggcaatgat tgtgaaagtc ggatgatagc acgtggagac
     1261 ctctggaagt tctcaatcac aaggggcatt aatacaccat caaaactcac ttcagggata
     1321 acgagaaaag cgttctgcgc aatgtcgggt gaattgagta gagcgtcaac ggtgtggtat
     1381 acgtgagaag gagcgtgcgt ttccggcgtt gaagagtaaa agcctctgat tggtgagcgt
     1441 acttcgagtc gtttaggttc tgttgttagt atccaccgcg ccgct
//
LOCUS       DAORIN010000122         1464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_30719_length_1464_cov_5.528034, whole genome shotgun sequence.
ACCESSION   DAORIN010000122 DAORIN010000000
VERSION     DAORIN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30719_length_1464_cov_5.528034"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PK109_03785"
     CDS             complement(<1..1034)
                     /locus_tag="PK109_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRH24679.1"
                     /translation="MALLDFLNRKHKDAEVSTKITPVLPKDIYAEGALDLADTIAPAA
                     LKVSSRELELGEKFARTFYTISYPRFLTDSWFTPVVNLTRVFDAGIFIHPVETEAALR
                     NFQRKVAEIQSQIAEREEKGLVRDPMLDTGYQDLENLRDSLQQAQERLFDVGLYLTVY
                     GDSKEELDKIEADVRGILDSRLIYTKPALFQQEQGFRSTLPLATDELAVNQKLNSSPL
                     SSIFPFVSFDLTSDHGILYGINRHNSSLILFDRFSLENYNSVVFAKSGSGKSYAVKLE
                     ILRSLMFETEVIVIDPEREYESLAEATGGRLFNISLSSEHHINPFDLPPVKEDENPSD
                     ILRSNIINLVG"
     gene            complement(1038..>1464)
                     /locus_tag="PK109_03790"
     CDS             complement(1038..>1464)
                     /locus_tag="PK109_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24680.1"
                     /translation="HSRRMDIRPYLALLEERTEVQQTELMRIQVREYIQFIKNFTEGA
                     DIMTKLFYIVVPYEPAAIKSVTSSLPLFGAKPSSQAEGLKQSFEEYRVQLEQRMSLVS
                     SSLAGSGVRAVPLGTEEIIELLYRSFNLGNQETPIKATV"
BASE COUNT          362 a          354 c          407 g          341 t
ORIGIN      
        1 cccaccaggt taatgatgtt tgaacggagg atgtctgaag ggttctcatc ttctttcact
       61 ggaggaagat cgaacggatt gatatggtgc tcagaagaaa gcgagatgtt aaagaggcgt
      121 ccgccggttg cttcagcgag ggattcatac tcacgctctg ggtcgataac aatgacctcc
      181 gtttcaaaca tgagggagcg gagaatctca agcttcacgg cgtacgactt acccgaacct
      241 gactttgcga acaccactga gttgtagttc tcaagcgaga agcgatcaaa gagaatcaaa
      301 gacgagttat ggcggttgat gccatagaga atgccgtgat cgctcgtgag gtcaaacgac
      361 acaaacggga atatagaaga tagtggagat gagttcagct tctggttaac cgcaagctca
      421 tcagtggcga gtggaagcgt tgagcggaat ccctgttcct gctggaaaag tgctggcttt
      481 gtgtagataa gacgggagtc gagaataccg cgcacatcag cctcgatttt atcgagctct
      541 tccttagaat ctccgtagac ggtgaggtaa agacctacgt caaagaggcg ttcctgtgcc
      601 tgttggaggc tatcgcgaag attctcaagg tcctggtacc cggtatcaag catcggatca
      661 cgcaccaacc ccttctcttc tcgctcggcg atctggctct gtatttcagc tactttgcgc
      721 tggaagttac gaagcgctgc ctccgtctct accggatgaa tgaaaatgcc agcatcaaac
      781 acacgcgtga ggttcactac gggtgtgaac caggagtcgg tgaggaagcg cggataagaa
      841 atggtgtaga aggtgcgagc aaatttctcg ccaagctcaa gctcacgaga ggacacctta
      901 agagctgccg gcgctatggt gtccgctaaa tcaagcgccc cttcggcgta aatgtcttta
      961 ggaaggactg gcgttatctt cgtcgatact tcggcgtctt tgtgcttgcg attgagaaag
     1021 tcgagaagtg ccataagtta tacggttgct ttgatagggg tctcttgatt cccgaggtta
     1081 aaagagcggt acagaagctc gataatctct tctgttccga gaggcacagc gcgcaccccg
     1141 cttcccgcaa ggctcgatga cacaagcgac atacgctgct caagctgaac acggtactcc
     1201 tcaaacgact gcttcaaccc ctctgcctgg ctgcttggct tggcaccaaa gagcggtagt
     1261 gagctcgtga ctgacttaat agccgccggc tcatacggca cgacgatata gaagagcttg
     1321 gtcatgatgt cagcaccttc cgtgaagttc ttgatgaact ggatgtattc acgcacctga
     1381 atgcgcataa gctccgtctg ttgcacctcg gtgcgctctt cgagcaaggc aaggtagggg
     1441 cggatgtcca tgcgacggga gtgc
//
LOCUS       DAORIN010000123         1444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_31496_length_1444_cov_4.507559, whole genome shotgun sequence.
ACCESSION   DAORIN010000123 DAORIN010000000
VERSION     DAORIN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31496_length_1444_cov_4.507559"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK109_03795"
     CDS             complement(<1..278)
                     /locus_tag="PK109_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24681.1"
                     /translation="MADTPITELVTLEDLKELLEENIALTKDTRKMLKRMRREAFIAG
                     VVKTAVWAVLLIASFWLSAKFLEPYLGMMTAGQEGAEQDYGALFEQYK"
     gene            complement(297..368)
                     /locus_tag="PK109_03800"
     tRNA            complement(297..368)
                     /locus_tag="PK109_03800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Arg,seq:cct)
     gene            complement(436..693)
                     /locus_tag="PK109_03805"
     CDS             complement(436..693)
                     /locus_tag="PK109_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRH24682.1"
                     /translation="MDKNVSIYTTPTCHFCQMSKEFFKANGVEYTEHNVAADQAKRQE
                     MIDKSGQMGVPVIFVGEEMVIGFDKNRLSQLLGLTEATTEV"
     gene            complement(728..801)
                     /locus_tag="PK109_03810"
     tRNA            complement(728..801)
                     /locus_tag="PK109_03810"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(767..769),aa:Gly,seq:ccc)
     gene            complement(803..1153)
                     /locus_tag="PK109_03815"
     CDS             complement(803..1153)
                     /locus_tag="PK109_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24683.1"
                     /translation="MMRRKRHTPKEYLIAGLLVLLLLFLLLQFWNIFRKEEIARHAAE
                     ETKRQLAALTLREETLKQDIADLHTERGQEASIRETYGVARPGEEVIIVVPADEGEAV
                     PTESWWDKLWSWMK"
     gene            complement(1172..>1444)
                     /locus_tag="PK109_03820"
     CDS             complement(1172..>1444)
                     /locus_tag="PK109_03820"
                     /inference="COORDINATES: protein motif:HMM:NF018161.2"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRH24684.1"
                     /translation="RMVEKLHQILPKQLQVTKIQAQALGRIISSRTLSAVRKDVTGYL
                     YGGDVSRKNKLLQKQKKGKKHRAQFEKNRVDIPEDVFMKMVQESEK"
BASE COUNT          328 a          390 c          358 g          368 t
ORIGIN      
        1 ctcttatatt gttcgaaaag ggccccgtag tcctgctccg ccccctcctg ccctgccgtc
       61 atcatgccaa ggtatggctc aaggaatttt gcagagagcc agaacgaggc gatgagaagg
      121 acggcccaga cagcggtttt aacgacgcca gcgataaacg cctcgcggcg catacgcttc
      181 aacatcttac gtgtgtcttt agtcagcgcg atgttctctt caagcaattc cttaaggtcc
      241 tcgagcgtca cgagctcagt gatcggggtg tcagccatgc taggtagtat atcaggtgct
      301 ctcggcagga atcgaacctg cattacctcg ttaggagtga ggagttctat ccattgaact
      361 acgagagcaa atcaaccaga gggagaatag cacaaaactg gccgctcgaa gcggccagtt
      421 ttgtaagaat attctttaga cttcggtggt cgcttcagtt aagccaagga gctgggaaag
      481 cctgttcttg tcaaaaccga taaccatctc ttcacccacg aagatgaccg gaacgcccat
      541 ctggccgctc ttatcaatca tttcctggcg ctttgcctga tcggcagcaa cgttgtgctc
      601 cgtgtactct acgccgttag ccttgaagaa ttccttcgac atctggcaga aatggcaggt
      661 cggggtcgta taaatgctga cgttcttatc catatatgtt ctcgttatgt cggttcatta
      721 tagcacttgg tgcgggatac gagaatcgaa ctcgtgcctc aaccttggga aggtcgcgtc
      781 ctgccactaa accaatcccg cattatttca tccagctcca gagcttatcc caccaagatt
      841 cggtaggaac ggcctcaccc tcatctgccg gtacgacgat gataacctcc tctccgggac
      901 gtgcaactcc gtaggtctca cgtatggaag cctcctgacc ccgctcggtg tgaaggtctg
      961 caatatcttg cttcaaggtc tcttcccgaa gcgtgagagc agccagctgg cgcttggttt
     1021 cttcggcggc atgccgcgct atttcttcct tgcggaagat attccagaac tggagaagca
     1081 agaacaggag aagcagaact aggagccctg caatcaggta ctcctttggg gtgtggcgtt
     1141 tgcggcgcat catccgccca gtataccaac cctatttctc actttcctgg accatcttca
     1201 taaagacgtc ctcagggata tcaacgcggt tcttttcaaa ctgtgcacga tgcttcttac
     1261 ccttcttttg tttctgaagg agcttattct tacgagagac gtcgccgcca tagaggtacc
     1321 ccgtaacgtc tttacgtact gctgagagcg tgcgggagga aatgatgcga cctagcgcct
     1381 gcgcctgaat tttggtgacc tgaagctgtt ttggaagaat ctgatgaagc ttctctacca
     1441 ttcg
//
LOCUS       DAORIN010000124         1377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_34228_length_1377_cov_11.894856, whole genome shotgun
            sequence.
ACCESSION   DAORIN010000124 DAORIN010000000
VERSION     DAORIN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34228_length_1377_cov_11.894856"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..485)
                     /locus_tag="PK109_03825"
     CDS             complement(63..485)
                     /locus_tag="PK109_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24685.1"
                     /translation="MYVEYESNNSWGSWWLEDSHWRALEAAGWKVQWAHLEKKYTAKG
                     YERDADGTPLLVPIGNSSLARKGEDGEYRWLGALATCAFRVGLGLCGAADEWKRITGM
                     DITAAGCACCGQPHTFTEYDDAGKFVRRGPFTSYEARW"
     gene            complement(785..861)
                     /locus_tag="PK109_03830"
     tRNA            complement(785..861)
                     /locus_tag="PK109_03830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(825..827),aa:Arg,seq:tct)
BASE COUNT          306 a          412 c          304 g          355 t
ORIGIN      
        1 ataacatacg gtttattgct cattttgttc tcctgtagtt ttcattactt gggtgttaga
       61 acttaccagc gtgcttcata actcgtgaac ggaccgcggc ggacgaactt tccagcatcg
      121 tcgtactctg tgaacgtatg cggttgaccg cagcacgcac accctgcggc ggtgatatcc
      181 attccggtga tgcgcttcca ttcatcggcg gctccgcaca agcccaagcc gaccctgaac
      241 gcacacgtcg caagcgcacc gagccagcga tattcgccgt cctcaccctt gcgcgccaaa
      301 ctgctgttgc cgattggcac aagcaaaggc gtcccatcag cgtcgcgctc gtagcccttg
      361 gcggtgtact ttttttcaag gtgcgcccat tgcactttcc aaccagcggc ctccaaggcc
      421 ctccagtgcg aatcttccag ccaccaggag ccccaagaat tgttgctttc gtattctacg
      481 tacatcctcg tttcctctta tcgtgcgaac ctggagtggg ggatgagagc tacgatagtg
      541 tcatgaccaa agattacatc cttctgcacc cccagttggg tgcaatggct atccgcttcc
      601 ttacgataga ttccgtcgtt cctgatgcgc ttcttcccct ccaccttggg ggagatgaca
      661 cgaaacacac tgccattagg caggtcacgg atagaaacag ttacattggc attcattacg
      721 ctactctcct tcgctggtga atagactcat ccacccggat gaatccgtta aaaaaatgtt
      781 aatgtggccc gcccggaggg attcgaaccc ccgaccgtcg gcttagaagg ccgatgctct
      841 atccggctga gctacgggcg gaagaaatta tggtacgtcc ttagcagcaa gcacaacctt
      901 tcgcggtact acaacctcgg tcacgccgag atgttccagc agtcggatat gggactcccg
      961 caattcccag tagcgatccc ggaggtcttt gtactggcgc tccatgtaat ccatttgtgt
     1021 atcaaaactt ctcgtgctta caaacatctc ttcgctcctc atttgacaat ggtggctatt
     1081 gctatcccat aacagctaaa atccacgcta aagagtatgt actcttctcc ttctttatag
     1141 gagcaatcaa actcctcagg aggaatcaga gcacagaact cagtatatac ttcctcgcgt
     1201 gttttaagac gtttctccgt ctcattccac gcacgaatcc cgggcactcc atctgctagg
     1261 aaatatttac ggacaatcct atcctgtgct tcttggctca tacatttatc gcacatctcc
     1321 cactcctttt gtttagccca tgcttcgccg ctctcctaga acccgcacga caggcgg
//
LOCUS       DAORIN010000125         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_35188_length_1357_cov_4.336406, whole genome shotgun sequence.
ACCESSION   DAORIN010000125 DAORIN010000000
VERSION     DAORIN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35188_length_1357_cov_4.336406"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PK109_03835"
     CDS             <1..461
                     /locus_tag="PK109_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24686.1"
                     /translation="AQGVRKPGAKLAAAVQTFTESDMILVRGKDGWRLSGAVLAHDWF
                     ATLPAGARSRAGRIARLLLRLVHGETSDTASFYIFRGLLEALPMLSEPEADAAETLAA
                     LRILRALGLDAGEMPGTTDTDYTKETLATVVAERRVFVMRVNRGIEASGL"
     gene            487..>1357
                     /locus_tag="PK109_03840"
     CDS             487..>1357
                     /locus_tag="PK109_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24687.1"
                     /translation="MAEPIEDSPLEKMRKRLYTQGGIEQFQEPTLREMQAQAPEHWKA
                     TPPPPAKPKSKISGAALFLGFAVGFFILAGGITAAVLFLGGRSVSSDHVEIAIEGPTT
                     VAGGEGVPLLITIKNANPVALTGASLTVQFPEGTMNADDTAKPQPHYSDSLGDIGAGE
                     SVTRTVRAAYFGTENQKVTLPITLEYRTGSSNAVFTKKAQYEFTISTSPVGISVTTLS
                     EVATGQPLTLSILVRSNASAPLPNVAVRAEYPFGFSETSATPEPISGKLFSLGTLSPG
                     EEREIRVTGTLAGQ"
BASE COUNT          293 a          380 c          386 g          298 t
ORIGIN      
        1 gagcgcaggg tgtgcgtaaa ccaggcgcga agttagctgc tgcggtacaa accttcacag
       61 agtccgacat gattctcgtg cgcgggaagg atgggtggcg cttgtcgggc gctgtgcttg
      121 ctcatgattg gtttgccacg cttcctgcgg gagcccgttc tcgtgcagga cgcattgcgc
      181 gcctcttgct tcgtctcgtg cacggagaga cgagcgacac cgcgtcgttt tatatattcc
      241 gcggccttct tgaagcgtta cctatgctca gcgaaccgga ggcggatgcg gctgaaaccc
      301 ttgcggcgct ccgtattctt cgagcgctcg gtctcgatgc aggagagatg ccaggcacta
      361 ccgacactga ctatacaaaa gagacgctcg ctacggttgt tgctgaacga cgtgtattcg
      421 tgatgcgtgt gaaccgggga attgaagcgt caggactcta acccgcacgg tatactgagg
      481 gacagtatgg ctgagcctat tgaagacagt ccgcttgaga agatgcgtaa gcgcctctat
      541 acccagggcg gcatagagca gtttcaagaa ccaacccttc gtgagatgca ggcgcaggcg
      601 ccagagcact ggaaggccac tccgccgccc ccagcaaaac cgaagtcaaa gatttcaggc
      661 gcagcacttt ttcttgggtt tgctgtcggg ttctttattc ttgccggagg cattacggca
      721 gccgtccttt tccttggtgg ccgttccgtt tcaagtgatc acgtagagat agcaatagaa
      781 ggacccacca ccgttgctgg gggagaagga gtaccgcttc tgatcaccat aaagaatgcc
      841 aaccctgtcg cgctcactgg tgcttcgctc accgtgcaat tcccagaagg aacgatgaat
      901 gcggacgata ccgcaaaacc ccagccgcac tatagcgact cgcttggtga cattggggcg
      961 ggggagagtg tgactcgtac ggtacgtgcc gcctactttg gtacagagaa tcagaaagta
     1021 acgctcccga tcacgcttga gtatcggacg ggaagctcaa acgctgtgtt caccaagaaa
     1081 gcccagtatg agttcaccat ctccacgtct ccagtgggca tctccgtaac gacattgtct
     1141 gaggttgcta ccggtcaacc gctcacgctt tcaatactcg tgcggtcaaa cgcaagcgca
     1201 ccgctcccca acgttgccgt gcgtgctgag tatccgtttg gattctctga aacctccgcc
     1261 actcccgaac ctatctcagg gaaactcttt tcgcttggta ccttatctcc gggcgaggag
     1321 cgcgagatcc gggtcacggg aacgttagcc ggccaag
//
LOCUS       DAORIN010000126         1294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_38395_length_1294_cov_4.477805, whole genome shotgun sequence.
ACCESSION   DAORIN010000126 DAORIN010000000
VERSION     DAORIN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38395_length_1294_cov_4.477805"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /gene="dnaB"
                     /locus_tag="PK109_03845"
     CDS             <1..1069
                     /gene="dnaB"
                     /locus_tag="PK109_03845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRH24688.1"
                     /translation="YAHYADLVSRKHLMRALIEASHAIEESAYDEARDTIEVLDAAEK
                     SVMSISDGTGSHKFTAIGDMVDDTFERIDALSKREDGIRGVPTGYDALDSMLSGLHKS
                     DLVILAARPSVGKTSLALDIARNAAVKHGVPVGIFSLEMSSEQLVDRMLAAESKVDSW
                     KIRTGAVRDQDDFAEIRNALERLSKAPIYIDDKPGNNILAMRSVARRLKREHGIGLIV
                     VDYLQLMNPTNTKASDSMVQQVTEISRSLKSLARELEVPVLALSQLSRAVEQRGGKPR
                     LSDLRDSGSIEQDADVVMFIHREDKNNPESDRQNIAEILIEKHRNGPTGKVDLVFDDK
                     RTSFQSLDTKGYGDLAGEFGM"
     gene            1075..>1294
                     /locus_tag="PK109_03850"
     CDS             1075..>1294
                     /locus_tag="PK109_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HRH24689.1"
                     /translation="MADRVEDLARAFERFPGIGPRQARRFVYHLLGVSSSDRARMADL
                     IAQLAADVRQCTECMRFGNGGNTVCKYCS"
BASE COUNT          291 a          355 c          335 g          313 t
ORIGIN      
        1 ctatgcgcac tatgcagact tggtctcacg aaagcacctc atgcgtgccc ttatcgaagc
       61 atcgcacgct atagaagagt ccgcatacga cgaagctcgc gacactattg aagtactcga
      121 tgctgcggag aagtccgtaa tgtccataag cgacgggacc ggttcacata agttcacagc
      181 gattggcgac atggtagatg acacctttga acgcattgac gctctctcga agcgtgaaga
      241 cggtattcgt ggcgtcccta ccggatacga cgcactcgac agcatgctct ccggactcca
      301 taagtcagat ctcgtgattc ttgccgcacg tccgtcggta ggtaaaacct cccttgcgct
      361 agatatcgcg cgcaacgccg cagtgaagca tggggtgccg gtaggtatct tctcccttga
      421 aatgagttct gagcaactcg ttgatcgtat gcttgccgct gagtcgaaag tggactcatg
      481 gaagattcgt acgggtgcgg tccgcgacca ggatgacttc gccgaaattc gtaacgcact
      541 tgaacgcctc tctaaggctc ctatctatat cgatgacaaa cctggcaata acattcttgc
      601 gatgcgctca gtcgcgcgtc gcttaaagcg tgagcatggt atcggtctta tcgtcgtcga
      661 ctacctccag ctcatgaacc ctacgaacac caaggcctcc gattccatgg tgcagcaggt
      721 gacagaaatt tctcgttcct taaagtcgct tgcccgtgag cttgaggtac cagtgttagc
      781 gctttcccag ctttcccgtg ccgttgaaca acgcggcggc aaacctcgtc tctctgacct
      841 tcgtgactct ggatctattg aacaagacgc tgacgtcgtt atgttcatcc atcgtgagga
      901 taagaataat cctgagagtg atcgccagaa cattgcagaa attcttatcg agaaacatcg
      961 taacggcccg acaggcaagg tggatcttgt cttcgatgat aagcgcacca gcttccagtc
     1021 ccttgatacc aagggatatg gggaccttgc cggagagttc ggcatgtaac tactatggct
     1081 gaccgcgtag aagatcttgc acgcgccttt gaacgcttcc ctgggatagg tcctcgccag
     1141 gcccgtcgtt tcgtatatca cctccttggg gtgtcttcct ctgatcgcgc ccgtatggct
     1201 gacctcattg cgcagcttgc ggcagacgta cgccagtgca cggaatgcat gcgcttcggg
     1261 aatggtggga ataccgtctg taagtactgc tctg
//
LOCUS       DAORIN010000127         1287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_38757_length_1287_cov_4.557630, whole genome shotgun sequence.
ACCESSION   DAORIN010000127 DAORIN010000000
VERSION     DAORIN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38757_length_1287_cov_4.557630"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PK109_03855"
     CDS             <1..170
                     /locus_tag="PK109_03855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRH24690.1"
                     /translation="ANGTKTRYAHLSSVAVSVGQTVDKGEKIGGVGNTGRSTGIHLHF
                     EVRGATNPFAN"
     gene            253..525
                     /locus_tag="PK109_03860"
     CDS             253..525
                     /locus_tag="PK109_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009803406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24691.1"
                     /translation="MTQQYSGPDHCNKEGATRLKLRLEEYWRERGYLVEMKIVPAGFV
                     TAMRSAREDVRSDMINGFPTKRAEPEVAKPPHYSATANEERQLQTA"
     gene            702..1067
                     /locus_tag="PK109_03865"
     CDS             702..1067
                     /locus_tag="PK109_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24692.1"
                     /translation="MSSKRVNLAKGTVVDALCEEMNALTIDCNDPAVRAATLARVIRL
                     TDWTSTTTRSRLYESLSNMMIRLGGTKHKRFTRYQRDQVNAAFDALQSTNDNVTVLAQ
                     RSVALTTADTTQQQAGASG"
BASE COUNT          334 a          318 c          331 g          304 t
ORIGIN      
        1 atgcgaatgg tacgaagact cgctatgcgc acctttcttc tgttgcggtt tccgtaggtc
       61 aaaccgtaga taagggcgag aagattggtg gcgttggtaa cacgggacgc tctactggta
      121 tccatctcca ctttgaagta cgtggtgcga cgaatccgtt tgcgaactaa tacctacatt
      181 gacttatatt tttatagtgt tacattgacc gcgtcctcaa agggacgtgg ttgcaatcca
      241 ggagcatgag taatgactca gcaatatagc ggtccagacc actgtaataa agaaggtgcg
      301 acacgcttaa agctgcgact tgaagaatac tggcgtgaac gcggctatct cgtcgagatg
      361 aaaatcgttc cggccggctt cgtcactgca atgcgttcgg cgcgcgaaga tgtccgctcg
      421 gacatgatca acgggttccc cactaagagg gcggagcctg aagtcgcgaa acctccgcac
      481 tactctgcga cggccaacga agagcgtcag ctccaaaccg cgtaacattg ttcttcgttt
      541 ctatagaata tgggcgtcct acattctccg taggacgcct gtttttattg caaagcggaa
      601 tagatcatcc actaactcac tttgactttt gtatatacga gacgtagtct cgatgaatcc
      661 ggcgcatacc gcgtacggaa caaaccatga ggtttgggga aatgtcctct aaaagagtaa
      721 accttgcaaa aggtacggtg gttgacgctt tgtgcgaaga aatgaacgca ctcacgattg
      781 attgcaatga tccggcggtg cgtgcagcga cacttgctcg cgttattcgc ctgacagact
      841 ggacgtcgac gacgacacga agccgcctct acgagtcact ttcaaatatg atgattcgac
      901 tcggcggcac gaagcacaaa cgcttcacac ggtaccagcg agatcaagtg aatgcagcgt
      961 tcgacgcgct tcaatcgacg aacgacaacg tcacggtgct cgctcaacga tcagtcgcgc
     1021 ttacaaccgc agacaccacg caacaacaag caggtgcgag cggataacgc tggcctactt
     1081 gtaacggcag tcgccggccc tttcacgggt cggcgatttt gtattcacat ctttttattt
     1141 atcttccgac gtacgcgtaa ataagagatc ttcaggaaga cgaaagagtc cctcgtcgtt
     1201 ttgtttttgt tcaaggaagt gcgcgatgaa cccgacggag cgtggaagta taaagagtgc
     1261 gttaaagaat tctgcatcaa tgagtgc
//
LOCUS       DAORIN010000128         1276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_39339_length_1276_cov_4.390663, whole genome shotgun sequence.
ACCESSION   DAORIN010000128 DAORIN010000000
VERSION     DAORIN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39339_length_1276_cov_4.390663"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PK109_03870"
     CDS             complement(<1..1262)
                     /locus_tag="PK109_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRH24693.1"
                     /translation="MKAYTFDGNMGENVIEGEIPEQYLEDAKKYRAEFIERIVENDDA
                     QMNAYLEGKEPTLEELKATLRKAVINNAIFPVYTGSALKNKGVQLVLDAVVDYLPSPL
                     DLAAVKGTNPKTGEEIERKASDAEPFTALAFKLQTDPFVGALTFFRVYAGSVTAGSYV
                     YNANTGTKERVGRIVRLQADKREEVEQVFTGEIAAMVGLKDTKTSHTLCDENNPIILE
                     QIKFPEPVVSLRIEPATKADQEKMGMAIRKLADEDPTFKVTSDEETAETIISGMGELH
                     LEILVDRMKREFNVVANVGKPQVAYRETIMGAADAEGKYIKQTGGKGQYGHVKIKMKH
                     LEPVDPEAKVAKNVTREDHFEFINNIKGGAIPGEFINPIEKGIREAMTRGILAGYPMV
                     DISVDLYDGSFHDVDSSEIAFKIAASMA"
BASE COUNT          260 a          341 c          343 g          332 t
ORIGIN      
        1 aatgccattg atgcggcaat cttgaaggcg atttcagaag agtccacatc gtggaaggat
       61 ccgtcataga ggtcgacaga gatgtccacc attgggtagc cggcgagaat accgcgggtc
      121 attgcctcgc gaataccctt ctcaattggg ttgatgaatt ctcctggaat agcacctccc
      181 ttgatgttgt tgatgaactc gaagtgatcc tcgcgcgtca cgttcttcgc gaccttcgcc
      241 tctgggtcta ctggctcaag gtgcttcatc ttaatcttca cgtggccgta ctgaccctta
      301 cctcccgtct gcttgatgta cttaccttca gcgtcagcag ctcccatgat ggtctcgcgg
      361 tatgccacct gcggcttacc aacgttagcg acgacgttga actcacgctt catgcggtcg
      421 acgagaattt cgaggtgaag ttcacccatg cctgaaatga tggtctctgc cgtctcttcg
      481 tcagaagtta ccttgaaggt tggatcttcg tcggcaagct tgcgaatggc catacccatc
      541 ttctcctggt cagccttggt agcaggctca atgcgaagcg acaccactgg ctcagggaat
      601 ttaatctgct cgaggatgat tgggttgttc tcatcgcaca gcgtatgaga ggtcttggtg
      661 tccttaagac ccaccatagc ggcgatttct cccgtgaaga cctgctccac ttcttcgcgc
      721 ttatctgcct gaagacggac gatgcgtccc acgcgctcct tggtaccggt gtttgcgtta
      781 tagacgtaag aaccagcggt aacggagcct gcgtacacac ggaagaaggt aagggcaccg
      841 acgaatgggt cggtctggag cttgaaggca agcgccgtga aaggctcagc gtctgatgct
      901 ttgcgctcaa tctcttcacc cgtcttaggg ttcgtaccct ttactgcagc gaggtcgaga
      961 ggtgatggaa ggtagtcaac gaccgcgtcg agtacgagct gcacgccctt gttcttgagc
     1021 gcggaaccgg tatagaccgg gaagatcgcg ttgttgatga ctgccttacg aagggttgcc
     1081 ttcaattcct cgagggtagg ctccttccct tcaaggtagg cattcatctg tgcgtcgtcg
     1141 ttttcaacga tgcgctcgat gaattcagca cggtacttct tcgcgtcctc aaggtactgc
     1201 tcgggaattt ctccttcaat gacgttctca cccatgtttc cgtcgaaggt gtaggccttc
     1261 atagtgagga ggtcaa
//
LOCUS       DAORIN010000129         1241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_41356_length_1241_cov_4.442664, whole genome shotgun sequence.
ACCESSION   DAORIN010000129 DAORIN010000000
VERSION     DAORIN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41356_length_1241_cov_4.442664"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK109_03875"
     CDS             <1..188
                     /locus_tag="PK109_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24694.1"
                     /translation="IRGDEADLFVPPGTRVTYVVGQEVEAAKSAEAAFKGLNLVDGSL
                     LYVDLRFDGKVYVKRRE"
     gene            201..1181
                     /locus_tag="PK109_03880"
     CDS             201..1181
                     /locus_tag="PK109_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRH24695.1"
                     /translation="MKSFWNELPKPFMALAPMADVTDPAYRRLIAETASPHVTWTEFV
                     SADGLFHTREKKGMQDEENPLVRDLLYTEGERPIVAQFFTSNPDTMEYAAALALERGF
                     DGVDINMGCPDKSIEKQGAGAAHMKDPARAKEVIAAALRGTQGKLPVSVKTRIGYNKV
                     EYTEWLPYVVDSGISALTVHLRTRKEMSLVPAHYELAGDIVSLVRKENPEVILSANGD
                     LLSVADAREKAALYGFDGMMLGRAIFGNPWLFSGKAPADFSPEERIQMLLRLAQYFDA
                     LRPQKHFAILKKHFKAFISGWDNAAELRARAMGTNSREELEAVLTGVQYD"
BASE COUNT          307 a          305 c          342 g          287 t
ORIGIN      
        1 ctattcgtgg ggacgaagct gatttgtttg tgccgcccgg aacgcgcgtc acctatgtcg
       61 tcgggcagga agtagaagct gcgaaaagcg ccgaagcagc gttcaaagga ttgaatcttg
      121 ttgatggttc gcttctctat gttgaccttc gatttgacgg aaaggtgtac gtaaaacgac
      181 gcgagtgata gcgtaatcac atgaaatcgt tctggaacga attaccgaaa ccattcatgg
      241 cgcttgcccc tatggcagac gttacggatc cggcctatcg ccgacttata gcggagaccg
      301 catctcctca cgttacgtgg actgaatttg tgtcagcgga cgggttattt catacacgcg
      361 agaagaaagg aatgcaggac gaagaaaacc ctctcgtacg cgatcttctc tacacggaag
      421 gtgaacgacc tatcgtcgct cagtttttta cgagtaaccc ggacaccatg gagtacgcgg
      481 cggcactcgc gctcgagcgg ggatttgatg gagtggatat caatatgggt tgcccggata
      541 agtcgataga gaagcaggga gcaggagcag cgcacatgaa agacccagca cgcgccaaag
      601 aggtgattgc tgcggcactt cgcggtacgc aagggaaact accggtctca gtaaaaacac
      661 gcatcgggta taacaaggtg gaatatacag aatggctccc gtacgtggtc gactctggta
      721 ttagcgcgct cacggtccac ttgcggaccc gaaaagaaat gtcactggtg cctgcccact
      781 acgagctcgc gggcgatatt gtttcgctcg tgcgaaagga aaatcctgaa gtcattcttt
      841 ctgcaaacgg tgatttactc agcgttgctg acgcgcgcga gaaggcagcg ctgtatggct
      901 tcgatggcat gatgcttggt cgggcaatat ttggaaaccc ctggctcttt agcggcaaag
      961 ctcccgcgga tttctcccct gaagagcgta tccagatgct ccttcgttta gctcagtact
     1021 ttgatgcgct tcgtcctcag aaacatttcg ccatactcaa aaaacacttt aaagccttta
     1081 tttctggctg ggataacgct gcggagcttc gtgctcgtgc catgggtacc aacagtcgag
     1141 aagaattaga agcggtgctt accggtgtac aatacgacta aggcttacgg taggagggaa
     1201 ttccatgttt attgcgccca cttccaacac ctcagaggcc g
//
LOCUS       DAORIN010000130         1179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_45406_length_1179_cov_5.248221, whole genome shotgun sequence.
ACCESSION   DAORIN010000130 DAORIN010000000
VERSION     DAORIN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45406_length_1179_cov_5.248221"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1179)
                     /locus_tag="PK109_03885"
     CDS             complement(<1..>1179)
                     /locus_tag="PK109_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRH24696.1"
                     /translation="IAVVSTSGGMTNELIHGVAASGKGVSFALAIGGDRFPVASLSQV
                     LALAEADGATKALAYFGELGGEDEYEIVELLKSKKFTKPVIAYIAGIIDEAFDEHMQF
                     GHAKALVARKDESARAKREALRAAGAIAPDTFPEFLAAFAQTPGSDIVAPMGTADELA
                     GRRESILSTRKIIDTEHAPTYVADGALVPQTTSYTAHILSALLGRSPKSPITVAFADA
                     VFSQLIDHGGAVSGAVNTMITARAGKDMVSSLAAGLLTVGPRFGGAINDAAKNWLNGL
                     SSGMSAKEYVESETKAGRLILGIGHKKYRIGIPDPRVAALSQFATLLKSHPRYDFARA
                     VEEVTTAKSGPLILNVDGIIAALFTDLLIECEGYTEAEVRALIDAEFFNALFILPRSV
                     "
BASE COUNT          272 a          321 c          308 g          278 t
ORIGIN      
        1 cgacggagcg tggaagtata aagagtgcgt taaagaattc tgcatcaatg agtgcgcgta
       61 cctctgcttc agtgtaccct tcgcattcaa taaggagatc ggtgaacagt gccgctatga
      121 tgccgtcgac atttaggata agtggaccac tctttgccgt agtcacctct tcaactgcac
      181 gcgcaaagtc ataccgcgga tgtgatttca ataatgtggc gaactgagaa agcgcagcga
      241 cacgcgggtc aggaatacca atacggtatt tcttgtgacc aataccaaga atcaatcgtc
      301 ccgccttggt ttcactttca acatattctt ttgcgctcat gccgcttgag agtccgttca
      361 accagttctt cgcggcgtca ttaattgcgc caccaaagcg cggaccgaca gtgagaagac
      421 ctgccgcaag agacgacacc atatctttac ctgcgcgtgc ggtaatcatc gtgttcacgg
      481 cgcccgacac ggccccaccg tggtcaataa gctgactaaa cactgcgtct gcaaacgcaa
      541 cggtgatcgg tgattttggt gagcgtccga gtaacgcaga aagaatgtgt gcggtgtacg
      601 acgttgtttg tggcacgagt gcgccgtccg caacataggt aggagcatgc tccgtatcaa
      661 ttattttgcg tgtggaaaga atactttcgc gacggcccgc taactcatcg gcagttccca
      721 taggtgccac tatatcgctt cccggcgttt gtgcgaacgc agcaagaaac tcagggaagg
      781 tatcaggcgc aatcgcgccc gcggcgcgta gtgcttcgcg cttagcgcgt gcgctctcgt
      841 ccttgcgcgc aacgagtgct ttggcgtgac caaactgcat gtgctcatca aacgcttcat
      901 cgatgatgcc ggcgatgtat gcgataaccg gcttcgtgaa tttctttgac tttaatagtt
      961 ccactatttc gtactcgtct tccccaccga gctctccaaa gtacgcgagt gctttcgtgg
     1021 caccgtctgc ttctgcgagc gcgagcactt gagacaacga cgctaccggg aaacggtcac
     1081 caccaatagc gagtgcgaac gagacacctt ttccgcttgc cgcaacgccg tgaataagtt
     1141 cgttcgtcat gccgcctgag gtagacacta cggcgatgg
//
LOCUS       DAORIN010000131         1107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_50989_length_1107_cov_6.679658, whole genome shotgun sequence.
ACCESSION   DAORIN010000131 DAORIN010000000
VERSION     DAORIN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50989_length_1107_cov_6.679658"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PK109_03890"
     CDS             complement(<1..518)
                     /locus_tag="PK109_03890"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HRH24697.1"
                     /translation="MQTPKKVLSVRSKILLTIVGSIVILGLAIYAISSVVLLQSYRDI
                     ENEAMVQNLRRATDAIAEFSGQQRIKLSDWAAWDEAYNYARDRDPEWAADTIYATGLA
                     NLDINAMMFADPDGNIIRLMTVDIPKRTEVSSDSLASYFTAHRDLITFNSLEGVTEGI
                     AMLPNGPLIIVSL"
     gene            632..928
                     /locus_tag="PK109_03895"
     CDS             632..928
                     /locus_tag="PK109_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24698.1"
                     /translation="MEPKPMNGWKVATGALVVLVVILLAMMWAQWSKQEHSLDNVLQE
                     GKEDVVEARAAIEMKCRGPQADEDDCQDALKDLADILKEFSKDVNAASTSGTAQ"
BASE COUNT          279 a          267 c          283 g          278 t
ORIGIN      
        1 agcgacacaa tgatgagcgg accgttcggg agcatggcga ttccttccgt cactccttcg
       61 agagaattga acgtgatgag atcgcgatgc gcggtgaaat aactggcaag actatcactt
      121 gatacttcgg tacgtttcgg aatatcgacg gtcatcagtc ggatgatgtt cccatcaggg
      181 tcagcgaaca tcattgcgtt tatgtcgagg ttcgcaagtc ctgtcgcata gatggtgtct
      241 gctgcccact ccggatccct atcccgtgca tagttgtatg cctcatccca tgctgcccag
      301 tcggagagtt tgatgcgctg ctgaccagaa aattctgcga tggcatcggt agcacgacgt
      361 aggttttgta ccatcgcctc attctcgata tcgcgataac tctgaagcag cacgactgac
      421 gatatcgcgt atatggcaag accaagaata acgatagacc ctactatcgt gaggagaatc
      481 tttgagcgga ctgacaatac ttttttggga gtctgcatag cgttctgcca taagtatata
      541 ccggtgatgg tgcatgaata tgcttcatga agcattcata ggatatgcgg tacaactccc
      601 cgcgtagtaa tcaatacaac aacgcatata tatggaacct aagcctatga acggatggaa
      661 ggtagcaacg ggagcactcg ttgtcctcgt cgttatctta ctcgccatga tgtgggcgca
      721 gtggagcaaa caggagcaca gtctcgacaa tgtacttcag gaaggtaaag aagatgtcgt
      781 ggaggcacgc gctgctattg aaatgaagtg tcgtggccca caggctgacg aagacgattg
      841 tcaggatgca cttaaggatc ttgccgatat tctcaaagaa ttcagcaaag atgtgaacgc
      901 agcctcaacg agcggtacgg ctcaataagc tacggttgta gaaaccgctc ccggttaccc
      961 gggagcggtt tctatttctc cctttgagaa atggagcggc accggtatag tgcgcgcatt
     1021 atcttcctag cacttacgca tgcctacgtt gactgattcg gccttccgca agattctcat
     1081 cgcccttgcg gtctatatca cttctct
//
LOCUS       DAORIN010000132         1105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_51164_length_1105_cov_5.128571, whole genome shotgun sequence.
ACCESSION   DAORIN010000132 DAORIN010000000
VERSION     DAORIN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51164_length_1105_cov_5.128571"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK109_03900"
     CDS             <1..193
                     /locus_tag="PK109_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRH24699.1"
                     /translation="GGGWYDRFLTKVPRSWLRVGFCYEHQFSSTPLLRQSWDQEMDAV
                     IVVSAAGARLIHSSATIAS"
     gene            190..531
                     /locus_tag="PK109_03905"
     CDS             190..531
                     /locus_tag="PK109_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24700.1"
                     /translation="MILSAFLFFVLSFITYFLIALFGHLTGGAPGTFWQSLTAAFKPL
                     PLLLILLSNLCFAAALWHGFKITAYAIPISIAIGVIASFTYSIAILHASVTPVKLLGV
                     MLILLGIYFLR"
     gene            537..851
                     /locus_tag="PK109_03910"
     CDS             537..851
                     /locus_tag="PK109_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HRH24701.1"
                     /translation="MKYKLVFTVPLVDADRVRMAIGEAGAGKSGNYSFASFSVRGIGR
                     FRPEEGATPAIGEVGKMEEVEEERVECQVDERYIEEVLAALRRTHPYEEIAYDVQVLE
                     DM"
     gene            854..>1105
                     /locus_tag="PK109_03915"
     CDS             854..>1105
                     /locus_tag="PK109_03915"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRH24702.1"
                     /translation="MHRITAIAAIGKTTNVLGKDNDLVWDIPADLERFRNVTRDHPII
                     MGRKTWESLPAERRPLPKRTNIVVSRQTDYDAPGAILVSS"
BASE COUNT          240 a          307 c          260 g          298 t
ORIGIN      
        1 aggcggtggc tggtatgacc gtttcctcac gaaggttcca cgctcctggc ttcgtgtcgg
       61 cttttgttac gaacatcagt tctcttctac tcccttgcta cgacagtcct gggatcagga
      121 aatggatgcg gtaatcgtcg tcagtgctgc cggagcgcga ctcatccact catctgctac
      181 cattgcctca tgattctttc cgcatttctt ttcttcgtac tgagcttcat tacgtatttc
      241 cttattgctc tcttcggtca cctcaccggt ggagctccgg gaacattttg gcaaagccta
      301 accgccgcat ttaaacccct ccccttactc cttattctct taagtaatct ctgcttcgcc
      361 gctgcgctgt ggcatggctt caaaattact gcgtatgcaa ttcctatttc cattgctatc
      421 ggcgtcatcg catcatttac ctattcaatc gccatactgc acgcaagtgt gacgcccgta
      481 aaacttcttg gcgtcatgct tatactcctc ggtatatact tccttcgcta gcccttatga
      541 aatacaaact cgtattcacc gttcccctcg ttgatgctga tagggtgcgt atggccattg
      601 gtgaagcggg tgctggaaag tctggtaact actctttcgc aagtttctcg gtacgcggta
      661 ttggacgttt ccgcccagaa gaaggtgcta cccctgccat cggtgaagta ggaaaaatgg
      721 aagaagtgga agaagagcgc gttgagtgtc aggtagatga aaggtacata gaagaggtac
      781 ttgctgcgct acggcgcaca catccctacg aagagattgc atacgatgtg caggtgcttg
      841 aagatatgta gccatgcatc gcatcaccgc tatcgctgct atcgggaaga caacgaacgt
      901 ccttggaaag gataatgatt tggtatggga tattcccgca gacctcgagc gcttccgtaa
      961 cgttacgcgc gaccacccta tcatcatggg ccgtaagacg tgggaatcac ttcccgcaga
     1021 gcgccgtccc ctcccgaagc gtacgaacat cgtcgtatca cgccaaactg actatgacgc
     1081 ccccggcgcc atactcgtgt cgtcc
//
LOCUS       DAORIN010000133         1090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_52468_length_1090_cov_5.465700, whole genome shotgun sequence.
ACCESSION   DAORIN010000133 DAORIN010000000
VERSION     DAORIN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52468_length_1090_cov_5.465700"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1090
                     /locus_tag="PK109_03920"
     CDS             <1..>1090
                     /locus_tag="PK109_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRH24703.1"
                     /translation="DHTLTLTPPETLSLDNLDRKAAERTRSILMFIGPLVHYFESFYL
                     PQSGGCTLGLRSVRPHLWALEKFGISIDAKTDRWPVSHKGLKPAEVIFYESSDTATIN
                     ALLAAARIPGRSVLKYASANYQVQEICGFLKALGVGVEGIGTTTLIIDGVRDIQKDVQ
                     YSVGEDPTDAMFFIAAAVATRSALRIERAPIEFLEPELIVLQKMGLTFRTEDVGISEN
                     GITKLVDLYIEPSVLKAFPEKIHARPYPGLNIDNLPFFAVIATQAEGTTLIHDWVYEK
                     RAVYYTELDRFGAETQLLDPHRINITGPSHLRAADITCPPALRPATILLIGALAAEGR
                     SVLKNVYSINRGYEDLVARLRSIGASIES"
BASE COUNT          238 a          321 c          268 g          263 t
ORIGIN      
        1 acgatcacac cctcaccctc accccacccg agacgctctc attggataac cttgatcgta
       61 aggcggcgga acgcacccgt tccatcctta tgttcatcgg tcccctcgtg cactacttcg
      121 agtccttcta tcttccccag tcaggcggct gcaccttagg actccgttct gtgcgtcctc
      181 acctctgggc gcttgagaag ttcggcattt ccatagatgc aaagactgat cgctggccgg
      241 tgtctcacaa aggattgaag cctgcggagg ttatttttta tgaatcaagt gatactgcaa
      301 ctattaacgc gcttcttgct gcggcacgta tcccaggacg tagcgttctt aagtacgcct
      361 ccgcgaacta tcaggtgcag gagatctgcg gattcttgaa agcgcttggc gtgggggttg
      421 aaggaatagg cacgacgacg ctcatcatcg atggcgttcg cgatatacag aaggacgtgc
      481 agtactccgt cggtgaagat ccaacggacg ccatgttctt catcgccgcg gctgtcgcca
      541 cccgctccgc gcttcgtatt gagcgcgctc caatagaatt ccttgagcct gaactcatcg
      601 tgcttcaaaa gatgggactc acgttccgca cagaagatgt cggtatttca gagaacggca
      661 tcaccaagct cgtagacctt tatatagagc catcggtgct gaaagcgttc cctgaaaaga
      721 ttcatgcacg tccgtaccca ggattaaaca ttgataacct tcccttcttt gcggttatcg
      781 ctacccaagc agaaggcacc acgcttatcc atgactgggt gtatgaaaag cgtgccgtct
      841 actacaccga gcttgatcgc ttcggcgcgg agacacagct cttggatccg caccgcatta
      901 acatcaccgg gccttctcat ctgcgggcgg cagatatcac ctgtccgcca gctcttcgcc
      961 cggcgactat ccttctcatc ggcgcgcttg cagcagaagg tcgttccgtc ttaaagaatg
     1021 tatacagcat taaccgcggg tatgaagacc tcgtcgcacg ccttcgctct attggcgcgt
     1081 cgatagagtc
//
LOCUS       DAORIN010000134         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_52631_length_1088_cov_5.141336, whole genome shotgun sequence.
ACCESSION   DAORIN010000134 DAORIN010000000
VERSION     DAORIN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52631_length_1088_cov_5.141336"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PK109_03925"
     CDS             complement(<1..664)
                     /locus_tag="PK109_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRH24704.1"
                     /translation="MENFDAEPAPEERKKFTAMMRIAMLIALVAVLSFTGGIIVAGQG
                     KDLLKGVPILSDGLDATPDPSADLSDFWKAWNTLNARFVQTHGTSTLPDSKEKVWGAI
                     EGLTKSFGDPYTVFMRPDDAKVFAEDIAGNFTGVGMEIGVNKDGILTVIAPLKGTPAE
                     KAGIRSGDLIIAIDEKSTDGMSTDEAVKLIRGEKGTNVVFRIYRDGEPSEITVTRDVI
                     NVP"
     gene            719..>1088
                     /locus_tag="PK109_03930"
     CDS             719..>1088
                     /locus_tag="PK109_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRH24705.1"
                     /translation="MITRHEREGLTWVDLESPTRDELRSVVEEFSIDAQVEEEIISPT
                     PYPLFVASSEYAYLILHFPTAEVSGGARNQEIDIIVGKNFLITARYEVVGCILTLHKA
                     FEAEELLGLPTEGGCAALVER"
BASE COUNT          243 a          300 c          274 g          271 t
ORIGIN      
        1 tcggaacatt gataacgtcg cgggtgacgg taatctcgct cggctcccca tcgcgataga
       61 tgcggaacac gacgttcgtg cccttttcgc cacgaataag cttcactgct tcgtccgtag
      121 acatgccgtc ggtggacttc tcatcaatcg caatgatgag gtcgccggaa cgaatacccg
      181 ctttttctgc aggcgttcct ttaagaggag cgatgacggt aaggatgccg tccttgttaa
      241 caccaatttc cataccgact ccggtgaagt ttcctgcgat gtcttcagca aatactttcg
      301 cgtcatcagg acgcatgaac acggtgtacg ggtcaccaaa gcttttggta agtccttcga
      361 tagctcccca taccttttcc ttactatctg ggagcgttga cgtgccgtgc gtttgcacga
      421 agcgtgcgtt cagcgtattc catgctttcc agaaatcact aagatcggcg gaagggtcag
      481 gcgttgcatc aaggccatca gagagaatcg ggacgccctt caatagatct tttccttgac
      541 ccgcaacaat gatgcctcca gtaaatgaaa gtacggcaac gagcgcgatc aacatcgcga
      601 tgcgcatcat ggctgtaaac tttttccgct cttctggagc gggttccgca tcaaagttct
      661 ccatatttct cacattctat catggggtca atcaccctca tgcgttatac tagtgcgtat
      721 gattacccga catgagcgcg aaggcctcac gtgggtggac cttgagtccc caacgcgcga
      781 cgaactccgt tcggtcgtcg aagagttcag tattgatgcg caggtagagg aagaaatcat
      841 ctcaccaacc ccgtacccgc tcttcgttgc ctcaagtgag tacgcctacc ttatccttca
      901 cttccctacc gcagaggtgt ccggcggcgc gcggaaccag gaaatagata ttatcgtggg
      961 aaagaacttc cttattacgg cccgctatga ggtagtgggc tgcattctca cgcttcacaa
     1021 ggcgtttgaa gcagaggagc tccttggtct tcctaccgaa ggcgggtgcg ccgcgcttgt
     1081 tgaacgtc
//
LOCUS       DAORIN010000135         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_53002_length_1084_cov_3.561710, whole genome shotgun sequence.
ACCESSION   DAORIN010000135 DAORIN010000000
VERSION     DAORIN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53002_length_1084_cov_3.561710"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..478
                     /gene="rplQ"
                     /locus_tag="PK109_03935"
     CDS             128..478
                     /gene="rplQ"
                     /locus_tag="PK109_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRH24706.1"
                     /translation="MRHHVKGRNLGRPKNQRVALKKSLMRSLIIHERIATTEAKAKEI
                     RPLIEKMVTRARTDSVANRRLTMARIGDADAVKKLFTTIAPRFKGQEGGYVRIVKRAV
                     KADGRKNAYIAFTK"
     gene            482..889
                     /locus_tag="PK109_03940"
     CDS             482..889
                     /locus_tag="PK109_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HRH24707.1"
                     /translation="MTTLERMPKDLRNRVDHTVAPVEYTVDAAGRTLGRVASEAASAL
                     LGKKSVHFLKTKVLPVKVTIINAKKLKLSEKRLSEKKYRYYTGHVGGLREFSLPMLLE
                     KKGVGEALRMAVDGMIPRNKLRKERMKRLTIKD"
     gene            893..>1084
                     /gene="rpsI"
                     /locus_tag="PK109_03945"
     CDS             893..>1084
                     /gene="rpsI"
                     /locus_tag="PK109_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRH24708.1"
                     /translation="MPELAYTTAVGRRKTATARVKLTPAAKGSVTVNGKTDVEYFKTR
                     ERVLVTKDAFHIDGVKGTFE"
BASE COUNT          272 a          301 c          285 g          226 t
ORIGIN      
        1 tggccttatt cgcaagacgg cgtcatctct taaggagctc gacggcgttg gcgacaaggc
       61 agttactgaa ataactgaag ccctcgagaa gctcggactc gagcttaagg ccgattaatc
      121 aactggtatg cgtcatcacg tcaaaggaag gaacctgggc cgccccaaaa atcagcgcgt
      181 tgcgttgaag aagtcgctta tgcgctcact catcatccat gagcgtattg caacgacgga
      241 ggcaaaggct aaggaaatcc gcccactcat cgagaagatg gttacccgcg cccgaaccga
      301 ctcggtggca aaccgccgcc ttaccatggc gcgtattggc gacgctgacg cagtcaaaaa
      361 gctcttcacc actattgctc cacgctttaa gggtcaggag ggtggctatg tccgcatcgt
      421 taagcgcgct gtcaaagcgg atggccgtaa gaacgcctat atcgccttca ccaaataatt
      481 tatgactacg cttgaacgaa tgccgaaaga cttgcgcaac cgcgtcgacc acaccgtcgc
      541 tccggttgaa tacaccgtag acgccgcagg gcgcaccttg ggacgcgttg cgtctgaggc
      601 tgcttccgcg cttcttggta agaagtcggt ccacttcctt aagacgaagg tccttccggt
      661 gaaggtcacc atcatcaatg caaagaagtt gaagctctcg gagaagcgcc tttctgaaaa
      721 gaaataccgc tactacaccg gccacgttgg tggtctccgc gaattctctc tcccgatgct
      781 tcttgagaag aagggcgtcg gcgaagcact gcgcatggca gttgacggaa tgatccctcg
      841 caacaagctc cgcaaggaac gcatgaagcg ccttactata aaagattaat atatgcctga
      901 actcgcttac accaccgctg ttggacgccg caagactgct acggctcgcg ttaaattgac
      961 gcctgccgca aaaggctcgg tgaccgttaa cggcaagact gacgtcgagt acttcaagac
     1021 gcgtgagcgc gtgttggtca ccaaggacgc tttccatatc gatggcgtta aaggtacctt
     1081 tgaa
//
LOCUS       DAORIN010000136         1055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_55593_length_1055_cov_4.737000, whole genome shotgun sequence.
ACCESSION   DAORIN010000136 DAORIN010000000
VERSION     DAORIN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55593_length_1055_cov_4.737000"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PK109_03950"
     CDS             <1..545
                     /locus_tag="PK109_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRH24709.1"
                     /translation="LGTAALNAGLIAGIIGFALVGLFMTLWYRLPGLVATVALVIYVI
                     ANIALIKTIPVTLTASGIAGLILSIGMAVDANVLIFERMKEELRKGDTVREALTIGFS
                     RAWPAIRDGHLTMIISSVILFWLGTSIVQGFALVFGFGVIISLISAVFITRIFLLAIL
                     PEDMKGWGRYLLGAGFKLKP"
     gene            550..>1055
                     /locus_tag="PK109_03955"
     CDS             550..>1055
                     /locus_tag="PK109_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH24710.1"
                     /translation="MFIINHRTFFFWLAGIITAGAIAAIVFFGLPLSIEFTGGSLVEI
                     AYTTERPSLESMKEKIHQAGLGEVTLRESGENGVLIKAPTLTPEEHDRMLMALSGDDA
                     HQFSEMRFNSIGPSLGDELAKKALWALGAVILAIVLYIAWAFRRVARPLTSWVYGGSV
                     VLILIHDVL"
BASE COUNT          208 a          263 c          286 g          298 t
ORIGIN      
        1 ctcttggaac ggctgccctt aacgcaggcc ttatcgcagg catcattggg tttgcgctcg
       61 ttggcctgtt tatgacgctc tggtaccgat tgccaggact ggtagcaacc gtggcgctgg
      121 tcatctacgt gattgcaaac attgcgctca tcaagaccat tcccgttacc ctaacggcgt
      181 ctggtattgc gggactcata ctctctatag gtatggccgt tgacgcgaac gtgcttatct
      241 ttgagcgtat gaaggaggag cttcgcaagg gcgacacggt gcgcgaggcg ctcacgattg
      301 gctttagtcg tgcgtggcct gcaatccgag atggtcacct cacgatgatc atttcatcag
      361 ttattctctt ctggcttggt acttctatcg tgcagggctt cgcgctcgtg ttcggattcg
      421 gcgttatcat ttctcttatc tctgcagtct ttattacgcg catcttcctg ctcgccatcc
      481 ttccagaaga catgaaggga tggggtcgct atcttcttgg agcaggattc aaacttaaac
      541 catagacgta tgtttatcat caaccaccgt acattcttct tctggctcgc aggcatcatt
      601 actgcagggg ccatcgcggc catcgtcttt ttcggtctcc ctctctctat tgaatttacc
      661 ggagggtctc ttgtggagat agcgtataca acggagcgac caagtcttga atcaatgaag
      721 gagaaaatcc atcaggcggg ccttggggag gtgacgcttc gtgagagcgg agagaatggg
      781 gtgcttatta aagcaccaac gctgactcct gaagagcatg atcgtatgtt gatggcgctg
      841 tctggagacg atgcgcatca gtttagtgag atgcgcttca actccattgg accatcgctt
      901 ggagacgaac tcgccaagaa agcactctgg gcgcttggag cagtgattct cgctatcgtg
      961 ctttatatcg cgtgggcatt tcgtcgtgtt gcgcgtccac tcacctcatg ggtgtatggt
     1021 gggtctgtgg tgctcatctt gattcacgat gtgct
//
LOCUS       DAORIN010000137         1052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_5576
            NODE_55885_length_1052_cov_5.220662, whole genome shotgun sequence.
ACCESSION   DAORIN010000137 DAORIN010000000
VERSION     DAORIN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867793
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 17:30:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 792
            CDSs (total)                      :: 750
            Genes (coding)                    :: 746
            CDSs (with protein)               :: 746
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55885_length_1052_cov_5.220662"
                     /isolate="AMR_MDS_5576"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PK109_03960"
     CDS             complement(<1..933)
                     /locus_tag="PK109_03960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRH24711.1"
                     /translation="MRLLIATPLYPPEPGGPATYAQVLEETLPKEGIEVTLAKFRDVR
                     GLPKIIRHIAYFFMLCSKGKHADALLALDPVSTGLPAALAARVLNKPFFVKVVGDYAW
                     EQGTQRFGVQGTLDEFTSERQLPFMVRMLLDIERWVALRAKKIIVPSNYLKSVVMRWR
                     IHSEKIVVIHNAVSITDIGTVPDAVAALPHPVIVSVGRLVPWKGMKGLIDAVEKIRET
                     VPVSLVIVGSGPEQEALTRLAHKKLGGAGFVLGELPHADALAVMQAADVFALNTAYEG
                     LSHLLIEALALGLPIITTPVGGNTELITHGKNGML"
BASE COUNT          242 a          310 c          262 g          238 t
ORIGIN      
        1 gagcataccg ttctttccat gagtaatcag ctccgtgttg ccgcccaccg gtgtcgtgat
       61 aatgggaagc ccgagcgcga gcgcctctat caacaaatgc gatagccctt cgtacgcggt
      121 attgagcgca aagacgtcag ctgcctgcat cactgcaagc gcatcagcat gcggcagctc
      181 tccgagaaca aagcctgccc ctccgagttt cttatgcgcc aagcgtgtca gcgcttcctg
      241 ttcaggtccg ctgccaacga tcacgagtga tactggcacc gtctcgcgta tcttctctac
      301 ggcatcaatg agtcctttca ttcccttcca cggaacgagg cgccccaccg acacaatcac
      361 aggatgagga agcgcagcaa cggcgtcagg caccgtacca atatctgtta tggaaacggc
      421 gttatgaatg acgacaatct tttcagagtg tatgcgccac cgcatcacca cactttttaa
      481 gtaattgctt ggcacgataa ttttctttgc acggagcgcg acccacctct caatatcaag
      541 cagcatgcgc accataaacg gaagctgacg ttcgctcgtg aattcatcga gtgttccttg
      601 taccccgaag cgctgggtac cctgctccca cgcatagtcc cctactactt ttacgaaaaa
      661 aggtttgttc aatacacgcg cagcgagcgc tgcggggagt ccggtagaaa cagggtcgag
      721 cgccaagagc gcatcggcat gcttcccttt actacaaagc ataaagaaat acgcgatatg
      781 gcggatgatt ttggggagtc cccgcacatc gcggaatttc gcaagcgtaa cctctatccc
      841 ttccttagga agggtctcct ccagcacctg cgcataggtt gcgggaccgc ctggctcagg
      901 cggatagagg ggcgtcgcga taagaagtcg catgctttat tttaccgtgc cagaaagcat
      961 ctggtcgaag agcgtcttca tctgtccggc gacaagcgtc cagtcatact tttcggtgac
     1021 gagcttccgt gcagtctcca ccaccttctc tg
//
