LOCUS       DAORIT010000001        55217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_74_length_55217_cov_53.778670, whole genome shotgun sequence.
ACCESSION   DAORIT010000001 DAORIT010000000
VERSION     DAORIT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 55217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55217
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74_length_55217_cov_53.778670"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLD99_00005"
     CDS             complement(<1..1158)
                     /locus_tag="PLD99_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25326.1"
                     /translation="MRKFLPHIIVLTIIVLAFLVVPTSYVLAQEQTTTNWLAAGAAKM
                     VLGSFDFVGVILSPLVIALVKVVSWITMLSGVVLNASIYYTVIAMSENIGRLPAIDIT
                     WTVIRDVANMGFIFMLLYSSIMTIVGRDKDTKGLIVKMVIAAVLVNFSLFFTKVIIDV
                     TNLLALTFYKAIAPGALLGPQNFLNAGLSNGIIDALGTQKLWQMYGSIDSSVVLTSAV
                     MTIIVLIVTTFIFSAMAVMFIVRYVALIFVLVLSPIMFLSMVFPGLSGYASKWKSTLI
                     GQAVFAPVFMLMTWVTVILIQGISATVFTGMTSESLGNALSSTSFTVGAVPTLVNYVM
                     IIIFLIASFTISKGVADQADGNINKLGKWAMGFAGGATAGTVGWAGRKSIGW"
     gene            complement(1155..3068)
                     /locus_tag="PLD99_00010"
     CDS             complement(1155..3068)
                     /locus_tag="PLD99_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25327.1"
                     /translation="MESKTKTFRKRLLSLVMVFVFGFSIFFSPIAAPKAQAFWGVGDI
                     NLESVPTIIDFLVNKVGKQLAQKMIDDIVQSTIKWANTGFDGNPAFITDTGSYFGNIA
                     NGVVGDAINDTSFGFLCSPFQAQIRLSLVKQYTQNTSNYQCTLSEIGVNIDNFYQDFN
                     QGGWDAWLKMTQEDQNNPQGAFLKAKLDIDSRLANTLSVSREDRVLNTGFMSKTTCLK
                     KNQPPNMATMARYYDADPEEAQNILNEYPNWNPNKEPTACLEEEVTTPGSVISNQLNK
                     ALGSGVDKLISADDIDSLASALLAGLLQRHVFNDKGKGLFNKNSIADTRNEIIDVDGD
                     NIPDGYDYNEDGILDICHHGQKDPNSDPSNDNCLMSGSVSNSPYFIPICKEIPGTIKA
                     LEVFNDFITRNDFRKENSVSWSNRMTTMNTAVDDLVSTISRYEVMAWDPVVFTLSQYS
                     KYIGGRISSLLQDDDLKFGGQSDVNELIKIRNNTLKTLQYSRGIKDRIGQCEDPNINA
                     INQVPAPTFEGDEVGNGGTTNTPAPTTQTPTTSNPSLSCAPSTSSTQVGTDMEWIMTS
                     SYPTGTTYRWFGDEIASSDTFFVEPLQVRYSFVGTKTANILATNPSGQAVSISCNGSV
                     SVTDSARNNSAGI"
     gene            complement(3052..3819)
                     /locus_tag="PLD99_00015"
     CDS             complement(3052..3819)
                     /locus_tag="PLD99_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25328.1"
                     /translation="MRTRVTLALFISFLLVLGASWQRADKEKSVFKVSAETPEINNNY
                     QVPEPNSVSDLSTERDTDDSENLTTTDLVGRQLITDYINLATNGQISEENITALADKY
                     ADSIADIKTAPKVSALDINVVSNAKANFQKYDLITTEIERRRINKISSIGSDPTKVNS
                     TDKSLEDAAKKISLAYETAAQELKNIEVPSSLLVLHTDLVNNYLSISSGMKSISDQSV
                     DSALSFAGVISARNNIGGGERIVEDIIKVLLDNGIQN"
     gene            complement(3914..4687)
                     /gene="rsmA"
                     /locus_tag="PLD99_00020"
     CDS             complement(3914..4687)
                     /gene="rsmA"
                     /locus_tag="PLD99_00020"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRH25329.1"
                     /translation="MYAKKSLGQNFLKSEKALNQIVEAGRLSPSDTVLEIGPGLGALT
                     SWLLYSGAKVIGVEKDDDLTLKLKEMFATEIESGQLTLMNEDILDLTPKWDRYKLIAN
                     IPYNITGAILEKFLSNSNQPELMVVLVQKEVAERIIARNSKESILSISIKIYGEPKLV
                     DTVKAGSFSPAPKVDSAILAIYNISKTFFEQNSVEEAHFFKILRAGFASKRKKLSSNL
                     GVVFDKDKVLETFKNLSLSENTRAEDVPLDLWKELSLKL"
     gene            complement(4712..6688)
                     /locus_tag="PLD99_00025"
     CDS             complement(4712..6688)
                     /locus_tag="PLD99_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRH25330.1"
                     /translation="MATLNERQQQAVDTLEGPLLILAGAGAGKTKTIAERIRNLVKHG
                     VTPSSILAITFTNKAAKEMRERVEKMLLEDTELSRPITMTEKPFVSTFHSLGVHILKE
                     NSQTLGISRHFTIFDKDDAKRAIKEALVNTGFDPKTHEPDRILSIISKEKGRDTTSEE
                     YMSHEEGGHFASTVKSVWGEYEKILKRDSALDFDDLLLKTVRLLRGNPKVRDYYRQVW
                     KYIHIDEYQDTNKVQYEIAKLLTSEEKNITVVGDIDQNIYSWRGADIQNILKFEKDYP
                     ETKIILLEENYRSTKTILEAANTIIKKNKIRVEKNLFTQSESGEKISLLSTFDEISEA
                     EFIATKAKSLIAGGESADEIAVLYRANFQSRVLEEAFLRHQVPYQLLGTRFFERREVK
                     DIISYLRAGLNPGALADVKRIINTPTRGIGKTTVAKIFAGDETTLPAGTRVKIADFRK
                     LLADIGEKANELKPSEVINHIIKSTGLEAEWRGGRDEDQDRLENAFELVAFASRYDHL
                     PQGEGIENFLKDAALQSDQDDLDRKKNEKENKNGVRLMTIHASKGLEFNVVFISGLED
                     GLFPHNKITRENITAEEREEERRLFYVAVTRARKKLFLTNAQVRTVFGQRETNLPSEF
                     IFDVPEELTEEEVWNEGSVPRRDTRRDILYKIDF"
     gene            6930..7877
                     /locus_tag="PLD99_00030"
     CDS             6930..7877
                     /locus_tag="PLD99_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRH25331.1"
                     /translation="MLVLAGTINLSTTEAQGEKVARAPQVVRSANSQNIELLKGSSNI
                     NPHPSQGTYLAVVDNSALEAKATPDPTTYADQGISGSGQISVYIVRDGDTLSTIAKMF
                     KVSINTIVWANDIKGGLIKPGQTLVILPISGVKHLVAKGDTIESIAKKYKADVAEILS
                     YNDLTKLSKLSAGDEIIIPDGEITSVVTYSSGSGTANTTVTSGYFARPVQGGKRTQGL
                     HGHNGVDIGGPMGTPVLASAGGTVIIARSSGYNGGYGLYIVVSHPNGTQTLYAHLSAV
                     NVSVGDKVTKGQVIGKLGNSGKSTGPHLHFEVRGAKNPF"
     gene            complement(7880..9403)
                     /locus_tag="PLD99_00035"
     CDS             complement(7880..9403)
                     /locus_tag="PLD99_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRH25332.1"
                     /translation="MSVSRVFSAQTTALSVSLVEIETDLSRGLYAFSIIGIPDQAVEE
                     AKDRVGAAIKNSGLTSPKQKNQKIVISLAPASEKKEGAIFDLPIALSYLLASEEVHFD
                     TKNKIFLGELSLDGTLRPTRGVLSISKFAKDEGIEEIYVPKDNAKEAALVDGIKVFGV
                     ESLKELLSHLEGRNLIKQQPKTFFGKSDEESHTDFSDVRGQERAKRGLLIAAAGGHNV
                     AMYGPPGTGKTMLAKAFASILPPLTFDEALEATSIHSVAGVLKQFIVNPPFRSPHHTS
                     SYVSLVGGGATPKPGEVTLAHRGVLFMDEFPEFERRVIESLRQPLEDKVISVARAKGT
                     ARFPADVILVAAMNPCPCGNFGFRGKQCVCTPSALQRYRRKMSGPIVDRIDLWLEVDR
                     VLPNDLSGETEGAKSHVYRSKVADARKTQAKRFGEKSKITKNAEMSARDLINFVPLSN
                     EARQTLNMAAERLNFSPRVYHRMLKVARTIADLDESDEIKREHILEAVEYRPKKFDL"
     gene            complement(9446..9811)
                     /locus_tag="PLD99_00040"
     CDS             complement(9446..9811)
                     /locus_tag="PLD99_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRH25333.1"
                     /translation="MISNTDQYKTKLEEEKKKIEGELATVAEKDPNHPTNWDPALGER
                     ENASADENEAADAIEDFEENMAITNSLEGRLKDVVAALEKIESGEYGVCKVCGNDIEA
                     DRLEANPAAPTCKTHIESV"
     gene            complement(9843..10907)
                     /locus_tag="PLD99_00045"
     CDS             complement(9843..10907)
                     /locus_tag="PLD99_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25334.1"
                     /translation="MPPEGSFQIKSSGETPPEITFVPKVAPQTTAQVSQPLAPAPAPQ
                     AVPPNPIAEAIAQNNVPPQEPEGTHLKQIRTFEGDVADAIRKQNESLISIQRKEEKKR
                     EAIQTLSPNAGQSESHFSFKPLVMALFTLIFISGGGYGAYYALTTYQEKTALPVVNAP
                     KNQFLGVDKTIEVDASTLGRQAVIDEVQKVRSESRGGDTLDQIQFSRGVEPNKELLAF
                     SDFLTRLNSHAPNPLVRAFDPLFMFGILGSSPSHSVLIVKLKSFENTFPGMLEWESRL
                     VEDMLPLFIDPALLDGVATNSVFKDKTIQNHDTRVLRNSYNQIVLLYGFFDNDTLIMT
                     DNENSFRTIINRLQAEKLSR"
     gene            complement(10943..11524)
                     /locus_tag="PLD99_00050"
     CDS             complement(10943..11524)
                     /locus_tag="PLD99_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRH25335.1"
                     /translation="MSVIKHKAQRVGIFIDTQNLYHSAKNLYHAKANFGAIIKEALDN
                     RVLVRAIAYVATTEGGDEKAFFDALSKVGIETRTKPLQIFAGGAKKANWDIDLAMDAV
                     KLANKLDTVIIASGDGDFVSLIDYLKHTHGTQVEVVSFGKSSSSKLIEAADDFLDLDS
                     NPKKYLLGVETRMQNRRQSQVKAPKEAPKETAE"
     gene            complement(11563..11829)
                     /gene="rpsO"
                     /locus_tag="PLD99_00055"
     CDS             complement(11563..11829)
                     /gene="rpsO"
                     /locus_tag="PLD99_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRH25336.1"
                     /translation="MISKTKKARVIKEARVHDKDTGSPEAQISILTARIEELASHLKK
                     NHKDNHSRRGLLGLVASRQAHMKYLEKKHPKRHSALVKKLSLKK"
     assembly_gap    11873..11972
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11973..12927
                     /locus_tag="PLD99_00060"
     CDS             <11973..12927
                     /locus_tag="PLD99_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRH25337.1"
                     /translation="DANAIMSQNMASTSELENLKRQFLEHTEITRGRSLKTVENYDRY
                     LTRFIEFAKVKSPKHITDSVIREYRLWLNRQPITKNKKNQDTLKKRTQNYYMIALRAF
                     LKYLASQNISTLSPERIELAKVSERHLDLITLNELERLMNAPDTSTLQGLRDKAMLEL
                     FFSTGLRVSELASLSRDLDIDADEFSIRGKGEKVRVVFISPHARDAVKLYLKKRNDLE
                     DAMFVNIVKDHTKTAPTRLTTRSIERIVKRYAIKAGISKKVTPHVIRHSFATDLLSNG
                     ADIRSVQMLLGHANIATTQIYTHVTDRALRETHKKFHSKRK"
     gene            13005..13241
                     /locus_tag="PLD99_00065"
     CDS             13005..13241
                     /locus_tag="PLD99_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25338.1"
                     /translation="MDFIVRNWLIWAVVAIVCYVVGMINQMSLMVRMARRGPLSGNVF
                     KGTGVTFLFFGIGGVSTFLLVLSVVVSLIDRFRS"
     gene            13365..14501
                     /gene="nusA"
                     /locus_tag="PLD99_00070"
     CDS             13365..14501
                     /gene="nusA"
                     /locus_tag="PLD99_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRH25339.1"
                     /translation="MFDLKVINSVLGELEEVRGIPRAKIIEAIEAALATAYKKEYGKR
                     GQLIRASFDMKTGQVEFFQVKNVVDDTKVFFDEEAELAEGDERVFYNPEQHMLLDDAK
                     KMKRDAKVDDEIIFPIESKGDYGRIAAQTAKQVIMQKIREAEKVSVLGEFGAREGEIV
                     SGTVQRIERGNIFVDMGRATGLLAYEEQIPGERFSQGERVRAYLYRVEESPRGVFLRL
                     SRSHPKFLEKLFEAEAPELANGAVVIKSVAREAGYRSKIAAQALDSHIDPVGALVGQR
                     GVRVSTVMSELRGEKIDVIEWQEEPKKFIEEALSPAKIMKIEMDEENKTAIVHVAESE
                     QSLAIGKGGQNVRLAAKLTGFKIDIQSAKGEELATGEATADNKE"
     gene            14504..15031
                     /locus_tag="PLD99_00075"
     CDS             14504..15031
                     /locus_tag="PLD99_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRH25340.1"
                     /translation="MNLWHEISPGTADEMNVIVEINKGSKNKYEIDKETGLIALDRVM
                     HTSQDYPFDYGFIPQSLWHDGDPLDVVLLTTYPLSPGILVKSRPVAVMHMIDGGEADE
                     KVIAVPVEDPRFDDIKDLSDINKHTLKEIQHFFSTYKKLQNKEVQVGDIEGKEVAIKA
                     FEESLELYSRKFSKN"
     gene            15102..15857
                     /locus_tag="PLD99_00080"
     CDS             15102..15857
                     /locus_tag="PLD99_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25341.1"
                     /translation="MKKLSIVLSLLLAIVAIPALAEDGQTSGRGVRDDFEAKKAEIQG
                     KIKENKDKMKEERELLKGEIKDKREEMKEDRKELRDDRQEDRQDKREDRREDRQDKRE
                     DRREDRGEKSVKVRADVAVRVLEATITRLETLATRIDSRIAKIGSTGTSTTEATGFVA
                     SARTDIGTAKTHIANIKGIDLSSDTASSTATSTNKVNRDKIKSEAKLAKEALVSAKQN
                     LMKALNAIKRIEASLRKEGKLPVATSTATTTTP"
     gene            15877..16173
                     /locus_tag="PLD99_00085"
     CDS             15877..16173
                     /locus_tag="PLD99_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25342.1"
                     /translation="MEPNQNNQPVQSNNENSIGPVIAIIIVLLMVILGGVYFWGERNN
                     SAQPASQEVVVNEANVDAVSGKIMQQSTSDESASIEEDLNGTDVDTVGADLNNL"
     gene            16242..17210
                     /locus_tag="PLD99_00090"
     CDS             16242..17210
                     /locus_tag="PLD99_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRH25343.1"
                     /translation="MSKHEHKHTNNLKIDRINGEAHITGEITLEALEEARGEAIKSLN
                     SRLEIPGFRKGFIPDNVLVKNIGEMKVLEEVAEIVLAHEYVHITSENKLSPIARPQIA
                     ITKLAPGIPLEFKITVALEPEFTLPDYKKIAKDSRKEDEDLKVDDKEVDAVLAEIEKR
                     GWKPELKEGEDLRTKAKENLLEEKKFRAKEKVRLTIVESLVKATDINIPNVLIQNELD
                     RMIAQFKDDVVKHGMVWDEYLKSIKKTEVDIRSEWNDKALARAKAELIMQKIAETEKL
                     VPTKEEVEHETTHLLTHYKDADPLRLRIYVFEQLKNQKVFEFLETI"
     gene            17235..17810
                     /locus_tag="PLD99_00095"
     CDS             17235..17810
                     /locus_tag="PLD99_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRH25344.1"
                     /translation="MSYLVPTVIEKSQFGERAYDIYSRLLRERIIFLGGPIDDAVANT
                     VIAQLLFLESEDPKKDITIYINSPGGQIQSGLAIVDAMNHIKPDVSTVCVGMAASMGA
                     TILSQGKKGKRFALPNAEVMIHQPLTGVEGQASDVEITAKHIIRLKDKLAKMMADVTG
                     QNYAKVAKDKDRDYWMTADEAKKYGIIDKIL"
     gene            17924..19426
                     /gene="rny"
                     /locus_tag="PLD99_00100"
     CDS             17924..19426
                     /gene="rny"
                     /locus_tag="PLD99_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRH25345.1"
                     /translation="MLLALAAGTGFGYLLRLLVALGKKNSVELEIQEMESRAKEEAQA
                     VIESANKEAKAVLDDAEVNIREREDRLLKNEDRLIKKEELLDKRQSDLDKEADYIKEK
                     VQEIKQIKERADELVVARSKELSRVASLSEEEAKNELFKRIEQSAEEDLLVRTQKLEN
                     QGEERLGERAKEILATSIQRLANSISNEVMSTNVPLASDEVKGKIIGKEGRNIKAFER
                     VTGVEVIVDDTPGAITISSFDPVRRAVAKLALEKLIADGRIQPAKIEEFVENAKNDIN
                     KVIKEKGEQAAFECGVYNLDPKLLSILGRLHFRTSYGQNVLEHSVEMAHLSGMIAEEL
                     GANVLVAKAGALLHDLGKALDHEVQGTHVEIGRRILQKFNVDEAVIKAMQAHHGEYPY
                     ETVESMIVQTADAISGGRPGARRDTVEHYLKRLEELENIATSFDGVEKAYALQAGREI
                     RVFVEPGKVSDLEARNMARNIALRIEKELKYPGEIKVSVIRENRVIEFAR"
     gene            19515..19787
                     /locus_tag="PLD99_00105"
     CDS             19515..19787
                     /locus_tag="PLD99_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRH25346.1"
                     /translation="MNKVAIVDAVHAKLGGTKVQAEEVVDTVIDSIVSTLKKGDEVSI
                     AGLGIFSTKTRAARQARNPRTGESISVPSMRVPKFRPAKALKDAVR"
     gene            complement(19837..21327)
                     /locus_tag="PLD99_00110"
     CDS             complement(19837..21327)
                     /locus_tag="PLD99_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRH25347.1"
                     /translation="MQTNEMAKIKEKLEIPEEVSRVSRVLSSTKFENYLVGGCVRDIL
                     LGKEPKDWDLTTVATPEEITPLFEHTFYENDYGTVGIVNDDPSTESLRVIEVTPFRTE
                     GTYSDFRRPDEVKWSKTLDEDLKRRDFTINALAYDIENREVIDLFDGREDLKNKLIRT
                     VGDPNERFGEDALRILRAIRFAAELNFAIESETVTAIEVKKDLLSQISKERIRDEFIK
                     IVMSDNPSVALGLATQLGLLKYISPELEKGVGVEQNKAHIYTVWEHLIRSLDYAAEKK
                     FPLHVRIAVLLHDIAKPHTRKMINGQWTFYGHEVVGAKIARNILTDLKFPKEIMEKVV
                     KLVRWHMFFSETETISLSAVRRIVKNVGPDLIWDLMDVRSSDRTGMGRPKAVTYRLRK
                     YHSMIELVCRDPISVKQLSVNGGDIMNLTNSNAGPHIGSILEIILSEVLEDPKLNERS
                     HLEERVKELYKLNGEELIKLGKEARKKNESEDDREVEEIKDKYKVE"
     gene            complement(21344..21415)
                     /locus_tag="PLD99_00115"
     tRNA            complement(21344..21415)
                     /locus_tag="PLD99_00115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21380..21382),aa:Arg,seq:tcg)
     assembly_gap    21416..21515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21517..21587)
                     /locus_tag="PLD99_00120"
     tRNA            complement(21517..21587)
                     /locus_tag="PLD99_00120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21553..21555),aa:Gly,seq:gcc)
     gene            21642..21712
                     /locus_tag="PLD99_00125"
     tRNA            21642..21712
                     /locus_tag="PLD99_00125"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21674..21676,aa:Pro,seq:cgg)
     gene            21735..21805
                     /locus_tag="PLD99_00130"
     tRNA            21735..21805
                     /locus_tag="PLD99_00130"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21767..21769,aa:Pro,seq:ggg)
     gene            21829..21901
                     /locus_tag="PLD99_00135"
     tRNA            21829..21901
                     /locus_tag="PLD99_00135"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21862..21864,aa:Pro,seq:tgg)
     gene            21954..22024
                     /locus_tag="PLD99_00140"
     tRNA            21954..22024
                     /locus_tag="PLD99_00140"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21986..21988,aa:Gly,seq:tcc)
     gene            complement(22043..22396)
                     /locus_tag="PLD99_00145"
     CDS             complement(22043..22396)
                     /locus_tag="PLD99_00145"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRH25348.1"
                     /translation="MVKYQKVELDRMLYAISDSTRRHILDELVRGEQSVGELAAPFKI
                     SLPAVLKHIRILKESELITTQKRGRRVMCNIRPDSLMRVSTWLAKYQKIWEGKLHELE
                     AQIRENRLNTDKKDT"
     gene            complement(22451..22813)
                     /locus_tag="PLD99_00150"
     CDS             complement(22451..22813)
                     /locus_tag="PLD99_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRH25349.1"
                     /translation="MTKTAKRSFGDIGENVACVFLEKHGFEIIERNYSRKWGEIDIVC
                     SKSGRIHFVEVKSVSSETGIRGEENIHPQKIKRLERTIQTYLTEKRTNMDWQLDLVLV
                     SINIFEKEAKVQIIQNVF"
     gene            23087..23251
                     /locus_tag="PLD99_00155"
     CDS             23087..23251
                     /locus_tag="PLD99_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25350.1"
                     /translation="MRQRRQTSKYKDWGSVVSAIEVFDQNIFHGCAAHKNNATDFVAE
                     WEKTTSPSGV"
     gene            complement(23306..24112)
                     /locus_tag="PLD99_00160"
     CDS             complement(23306..24112)
                     /locus_tag="PLD99_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRH25351.1"
                     /translation="MQIISWNVNGIRAVHKKGLFLPLIEEYKPDIVALQETKAEQGQA
                     VIDLPEYKEHWNSSRTRKGYSGTAIFSKNEPISVITDFPAQIVEKYKLIEDEYGNPNE
                     EGRVITAEYKDFYLINSYTPNSKPDLTRLKLRHKKWDPAFLEYVLSLEKMKPVIFTGD
                     LNVAHTEDDLAKPKDNVGEHGFTIEEREGVDKMINAGFADAFRMFTKGNGHYTWWHYF
                     SKSRERNVGWRIDYFFVSNKLKDKVESVNILSHILGSDHCPIQITLKDIK"
     gene            24137..24604
                     /locus_tag="PLD99_00165"
     CDS             24137..24604
                     /locus_tag="PLD99_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25352.1"
                     /translation="MKFIVPVVVLFLILLGGWLFLRKPDSTTKEIRQEVTTVSNGVQV
                     TKTDISKSGSMLPEGFPKDIPVEMANIKDSYRAYYEKVKATQYTVSYISKQSRDTLWK
                     TYSDFMNGKQYTVDQSSSRSLGQISGVKGDDSMSVVITSSGGVTLVQIVYLDR"
     gene            24606..25100
                     /locus_tag="PLD99_00170"
     CDS             24606..25100
                     /locus_tag="PLD99_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25353.1"
                     /translation="MPRNSNLGPLLIILVFLVALVGGYAIREDRWPGLPWSLGLAPTK
                     FTLDAEAPKGQLISGFPEDLINMTKDARIEQSAKYFLAEGVGKSLVLTTTYKTKASIS
                     ELFAAYIEKLTAEGYTLTKTSSGTGESEIEAFNSVQSISINISVSSNLEKEVRVDVRK
                     AVVQ"
     gene            25141..27327
                     /locus_tag="PLD99_00175"
     CDS             25141..27327
                     /locus_tag="PLD99_00175"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRH25354.1"
                     /translation="MKRLFLVVGLIALSLGVGQAYAWVEPTLAPPNGNVTAPLRLDAN
                     GRLGIGITSPGKLFEVMSEIVTRASSASGGVASSFSSDSTGAQIWVNSAYDPASPSTG
                     WGRRVTIAPNGNVGINNQFPSARLHVNLDANREIRVGTGISTALTDASVFGPVLSFSR
                     GSDGADDIAGIFQYTTSGAANRLAIASRDDIVFAAGGGSTYPSAPERMRINSAGNVGI
                     GTSAPNEPLEVANSTNGGRLIVSDGAGASRRTLLLVSPTATNNFGRVESYNYGASTGV
                     PLYINSSGGGNVSVGNLAPTYKLTVAGDVNVPTGSCYRVNGVCITSGGVSGSGTVNTL
                     SMFTAASTLGDSRLSQQADGSVRTTSPTGWVDIGPKNAGFNHIYTDRPATIFNTGVWT
                     ISGIFSAYNLDNLRLQTNGIDRITALASNGNVGIGTAAPTQRLTVNGNLNKLGSWIIG
                     DANWSPQGLEVHNSSWDGVSNNNYGAVAGGKLFAYNGLQSGGATGAEATDGQLYVAGT
                     SMLMGNVGIGKNNPSNTLDVAGGMNVELNADAIYAGYVRNTHINGNGLSGEGGIYGVR
                     GLANNGSGSGVYGQGAAGAAARGVVGAANDGYGVYGISTFGYGIYCAAAGTGFCGGTR
                     AWTNTSDIRLKKNVSTITSGLDKVLRLRGVNYTWKNDETNKKNIGFIAQEVMEVLPEV
                     VNKNNEGYYTVNESSVNAVLVEAVKELKAENDDLKARLERLEAKLK"
     gene            27333..28739
                     /locus_tag="PLD99_00180"
     CDS             27333..28739
                     /locus_tag="PLD99_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25355.1"
                     /translation="MKRLLITASFFLLATGFFSTPASAQTVGLSGFAWSDLPQSTAGG
                     VDRGAGWISFSGPGYGVIANQSTGNLSGYAWSEHYGWLSFNSAEVAGCPSGTCQPNIN
                     PSTGVATGWARFINAPAGTSGPWNGWVRLSGSNYGVTYSSGTQTFSGYAWGDSDIGWM
                     RWGGPGYTVTMSPLTPPEATINIVTVPANATWTINPGSIGGTGNGTATVTPSQAGTSY
                     TVTGGAAPSGYDFPPTITNSQGGGSSALILPGTSITFTITYTQAFNYALSNSGNVDVQ
                     KAGIAQSGQNVITATHSAGTGQAVTLSASGLPSGASIGFSGQGCAPNAGTPCNYTATI
                     SVLPSTVSGTYPITITGSPLGRTTSFNLVITDSPNMIISCSANPSTAQVGQDVTWTAT
                     IVNDPNNNSPYTYQWAGDGVPSDQTIEITATQSFITSYSTTGIKQIQATVWDDVRNQA
                     LCPTSYINVGVNPGFGEF"
     gene            28760..30502
                     /locus_tag="PLD99_00185"
     CDS             28760..30502
                     /locus_tag="PLD99_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRH25356.1"
                     /translation="MHIDETELKRLVLDSGLVSKKDIEDAAKFSEENGQSLGKVLVSR
                     GVITEDEFRRLQSYILGIPFVDLKNQKLNIEVLSLIPEPIARNHNIVAFKKTSTNLEV
                     AMLDIEDMTAIDFVRKKVGLKIDARLTDNESMKWALVSYQKSLKDEFGSVIEKVAGSI
                     KAGSAKGDENASDLKKLAEDVPVVRVVDTLLKHAIVQDASDIHIEPEDEQVLVRYRID
                     GILHDSMVLPKNTAAGITARIKVLSNLKLDEKRVPQDGRFKIDLDGEKVSFRVSTLPT
                     YYGEKTVMRILRENASGFTLESLGFHGYGLETIHNAIKLTTGMILTTGPTGSGKTTTL
                     YTVLDLVNTPDVNISTIEDPIEYQMKRVNQTQVRPEIGFTFAQGMRTLVRQDPDIIMV
                     GEIRDNETAALAVNAALTGHLVLSTLHTNSAAGAIPRLLDMQVEPFLLTSTLQVIVAQ
                     RLVRRLGQGKKKYNLNKTEIESLGHLIDLNRLLTMLKAEKVVPKDADWKDVPFYKPNP
                     EDDNEGYQSRIGIHEILKVTEPIKALILKNAPGKEIEEVGKKDGMMTMLEDGIYKAVL
                     GQTSIEEVLRVISE"
     gene            30504..>31597
                     /locus_tag="PLD99_00190"
     CDS             30504..>31597
                     /locus_tag="PLD99_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRH25357.1"
                     /translation="MLYTYTAKNAKGEVYTGDFEAKSKIEVYTKVRDEGGRVLGVREK
                     GYVNLNLSLDSMFSRINSRDKIVFAKNLSSMLSAGLSVTRALEVMEKQARKNALKKLL
                     HDLAQDVSKGIPFSEALRKKPKVFPPIFVSMALAGEESGNLSDSLGVIASQMEKSYTL
                     TKRVKGAMIYPVVILSLMVVISILLLIYMVPTLTSTFSGLGVELPITTRAIIAASDFM
                     IRHTLIFIATIILSIVLVFAFARSKAGGRLLESLFLKLPVVGLIIMEVNSARTARTMS
                     SLLSAGVPIVNALEVTESVVPNHLYKIALIDARNSIQKGETISTVLARYEKIYPPFIT
                     EMTAVGEETGKISEMLLNVALFYEEDVDDKT"
     assembly_gap    31598..31697
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31698..31937
                     /locus_tag="PLD99_00195"
     CDS             <31698..31937
                     /locus_tag="PLD99_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25358.1"
                     /translation="VGGTPAGVLLASQNLVRDGINWTVTTTVVNVDDPFDGIAPTDTK
                     PADYKLVEVMVGCDTCKNFSPVAVTGIVAPRNLEP"
     gene            31937..32545
                     /locus_tag="PLD99_00200"
     CDS             31937..32545
                     /locus_tag="PLD99_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25359.1"
                     /translation="MPKQGFTLVEFVMAVAIMISIVFGVTTLARDVLVINTSATQNLV
                     AQSDARKALKSIIAELRTAAPSSTGAYTIESASSTSVIFFADLDNDNRTERIRYYLDY
                     PTRTLRRGVVVPTGNPPSYVLGQETYTTLSSDIVISTTTPVFEYFNTNYAGTSSPLTY
                     PIDVSVIRLVRVRLIIDKDPNRAPLEQITTSEVTLRNLKDNL"
     gene            32542..33987
                     /locus_tag="PLD99_00205"
     CDS             32542..33987
                     /locus_tag="PLD99_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25360.1"
                     /translation="MKKEKGFLIIIVLVFASISIAAIIGLISFTNINIMLARRAVVSE
                     QAFQLAEAGLEYYRWHLAHDQNDFTDGGGAFPHLHNFYDRNGNLLGQYELTITPPITG
                     STLVGMRSTGIPLTDPTVRRTVTSSLGKPSFARYALVSNSDMRFGQGTLVIGPTHSNG
                     GIRFDGVAQNLVTSGVANYNDPDHTGNNEFGVHTHVNPPPGSGVNGTFRANEAPPTNP
                     VPNRPDVFLAGRRFPVPTVNFAGITADFTNLKSLAQSNGRYFASSTAQGYQVTFNTND
                     TYTVHRVTSLRPAPNNCTNTAGQTGWGTWTASTTVLVGTYANPNNGVIYMEDHVWVEG
                     QIDTARVTLVAAATSTGVQRSITVNNNLLYTNYDGQDVISLIAENNINTGLFSLDNLR
                     IDAALIANSGRIGRYYYEADCAPNNVRSTITLYGMIGSYLRYGYAYVDNTGYINRNLI
                     YDASLLYAPPPSFPLTTNTYQIVSWQETIND"
     gene            34010..34765
                     /gene="tpiA"
                     /locus_tag="PLD99_00210"
     CDS             34010..34765
                     /gene="tpiA"
                     /locus_tag="PLD99_00210"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRH25361.1"
                     /translation="MKYILAANWKSHPSNADEAKKLLVGLVKNGANFKKLHTIIAPPN
                     TFLELVNSKIRSIGVLASQDMSLLPKGTYTGETTAEMLKGLGVRVSIVGHSERRKLGE
                     TNTLVAAKVSQAIKEGIVPIVCVGEDVHDTEGGYYEVLNSQIRNSLEGLKKTDVKKLV
                     VAYEPLWAIGKSAKDAMQPGDLGQMVVYIRKVLTEMYGRTAADQVTIIYGGSVEPGNA
                     KELLSSGAQGFLVGHASLNSKSFAEIAKAILNK"
     gene            34762..35688
                     /gene="pgk"
                     /locus_tag="PLD99_00215"
     CDS             34762..35688
                     /gene="pgk"
                     /locus_tag="PLD99_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRH25362.1"
                     /translation="MKSVREIGDISGKKVLVRVDWNVPLVDGLVRDDLRIKRSLDTVN
                     FLVEKGARVTLMTHLEPEERTVEVLKHFLPTGVEMLENLRQNKGEKDNSEEFAKELAS
                     NQDYYVNEAFSASHREHASIVLVPKFLPSFAGLGFINEVENLSKAFSPKHPFLFILGG
                     AKFETKLPLVKKFLNIADGIFIAGLNAKPAFEMGFDKEPKITLPIGDISAPDLTSENL
                     DILNEKIENSEFIVWNGPLGKYEDGLKEGTLRLAQMLGNSGKEVVVGGADTLAAIKEL
                     DISDKFTFVSSGGGAMLDFLASGTLPGIEALS"
     gene            complement(35685..36017)
                     /locus_tag="PLD99_00220"
     CDS             complement(35685..36017)
                     /locus_tag="PLD99_00220"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRH25363.1"
                     /translation="MENCIFCKIGRKEIPSKTVFEDDKFIAFLDINPVREGHTLLVPK
                     DHYRWFTDLPNDLYDELFRAAKTLALKLKDDYHADYVRLGIVGKDVAHTHIHLVPQKM
                     EEEGGPRI"
     gene            complement(36023..36964)
                     /locus_tag="PLD99_00225"
     CDS             complement(36023..36964)
                     /locus_tag="PLD99_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25364.1"
                     /translation="MRKTLEKGAFVEWLLTFFAISIVIFAILIPKSMRFDVSGWDSTL
                     KGRNSTYQDDTKSTNYTSSSRSSGTLRLERGNGKYENDPMSEYIVIENNGSNPVNITG
                     YRLENGKSARSYTVGSQYLHYASDVGVIPQGAKIVSPDGRSHLESIVLNRGERAIVIS
                     GGPGNLTSYPLTSFKENSCTGYLEETYSFPKGLDKSCVRPSKEVGYTSLDIKCKRYID
                     SMQSCHTPEYGGKDEDGEVCRDCVDGEKGLSSMCIAYVKSHFSYEGCLANHIGEKNFE
                     GKTWHVYLHRPWEMWAEEEETISLFDSSGVLVDSISY"
     gene            36983..38599
                     /gene="recJ"
                     /locus_tag="PLD99_00230"
     CDS             36983..38599
                     /gene="recJ"
                     /locus_tag="PLD99_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRH25365.1"
                     /translation="MSLDDILHKLLHDRGVTDKEDFLVPDYSKLHDPLLMPGMEKSRD
                     RILRAIKDGEHIFVFSDYDADGIPGAVVMKDFISRIKYDNVSFYIPHRHDEGFGLNTD
                     AVDEANKRGAKLIVTIDCGTADSVEVAHANSLGIDVIITDHHEANDLPDAYAIVNPKI
                     KGSRYPFDGLCGAGVLYKLIQAIYMKESFGLPIGVEKWSLDMVAIATLSDMVPLVGEN
                     RILSYFGLQVLRKSPRPGIQALCKKLGMDQRFLNEDDIGFMITPRINAASRMGKPEDA
                     FNLLATNDPREAEELALHLETINNERKGIVASIVKDAKKHLSERESIGKVIVIGNPEW
                     RPALLGLVANSLVEEYERPAFVWGRDGDDKLKGSCRSYNGYDLFELMNHAKESFVEYG
                     GHAGAGGFSVTLESLVTLEENLSNALLKLEMTEGKTHNSLEIEMKDVGEGLYRVLNKL
                     APFGTGNPKPVFRINNAEIKSLKQFGKAKDHLELTLTPNIKAISFFSNPESYSLLPTT
                     TLCNLDVHLEKSYFRGRPDLRLRIVDIISS"
     gene            38596..39075
                     /gene="rnhA"
                     /locus_tag="PLD99_00235"
     CDS             38596..39075
                     /gene="rnhA"
                     /locus_tag="PLD99_00235"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRH25366.1"
                     /translation="MITIFTDGAARGNPGGGGWGAIIAKEFEVHEIGGKDPYTTNNKM
                     ELTAAIEALKTLEFGVEVELYTDSEYVMKGMTEWVHNWQKNGWRTAAKKPVENQDLWK
                     DLVSASEGKNISWKYVAGHSGNEANDRCDEIATKYADHLSVELYAGSRGEYPISLKV"
     gene            39167..40507
                     /locus_tag="PLD99_00240"
     CDS             39167..40507
                     /locus_tag="PLD99_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRH25367.1"
                     /translation="MFVASAIYVLDRNVLILITLVAASLGILRYDIKDFHELVPAGNI
                     GIVVSEPENRDKDTRYTVLTDTGEKVLVTDEIYSKVLYGDEVRLTGTPKILKPSGYAD
                     YLAKDDIFYTLERAKVEILSHDNGNGIKGALIKVKGFLIDKIKNILPEPEASLLSGLI
                     VSGKQALPKETLESFKDAGVVHMVVLSGYNITIIAEFLLIVFAFLGSRFSGFIALVGV
                     VLFTIMSGAPATVVRGAIMVSFLLFGRLMKRPGSPQRILLFTAVLMLMHNPKILLYDP
                     SFQLSFLAVLGLIYGAPIIQDKLAKLALPAHGRASGHSRAMPTWLTELLATTLATQVF
                     VLPLLLYSMGNFSLVFLFANLLILPILPVVMLIGFVGTLLSFVLGVLAMPINFITHTF
                     LAYILKIADLFGNLPFSSLEIENFPLWGSLVMYLLIFIIVWHSRNSSPHSPSLG"
     gene            complement(40455..41045)
                     /locus_tag="PLD99_00245"
     CDS             complement(40455..41045)
                     /locus_tag="PLD99_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HRH25368.1"
                     /translation="MKTFEEKKFEIEDLVGISKKTIDEHLKLYAGYVKNANLIMERMS
                     VLDSAKDSYIAGELFRRFSFEYNGMRNHEIYFSSLSGGAKELNKESELGKRLLELFGS
                     MDTFLTEFKALALTRGIGWAILWYDKTDDRFLASWVDEQHIGQLQGATPLLALDMWEH
                     SYCLDYTPSEKKNYVEAFFSNLNWESVEMNFENARR"
     gene            41044..41877
                     /locus_tag="PLD99_00250"
     CDS             41044..41877
                     /locus_tag="PLD99_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRH25369.1"
                     /translation="MYSIIAVLVFSNLFVWSRVYSRSGGELKVYFLDVGQGDAILIES
                     PTKGRVLIDGGPDRKVLSELGRVLPFGDRRIDVVIESHPDADHITGLVEVLNRFNVDT
                     FVSTGMRSVNSLDETLDGVIKEKEIHKVLGKRGMSLDLGGGAILTLLYPSGSIEGLET
                     NESSIVSKLTLGDIDFILTGDSTKLAEYKLLSLNKELLDSEVLQVGHHGSETSSSLLY
                     VQAVSPLYSVISSGKDNRYGHPHKVTLDTLNKVETNILNTADVGRILFKTDGEEIEVE
                     Y"
     gene            complement(41874..42116)
                     /locus_tag="PLD99_00255"
     CDS             complement(41874..42116)
                     /locus_tag="PLD99_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HRH25370.1"
                     /translation="MAKVCPVLKKGSKMGGGYSNRTRATQFNPTGMVRKYPNLQKKRI
                     YVPELKKTITLTLSTRAIKTINKRGAHSVLKKAGII"
     gene            42161..42763
                     /locus_tag="PLD99_00260"
     CDS             42161..42763
                     /locus_tag="PLD99_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRH25371.1"
                     /translation="MEFIFQIAILIMSIVIHEVAHGYAARYYGDLTAEYEGRLTLNPV
                     KHIDPVGSVFVPLVSYLFGGFVFGWAKPVPYNPYNLKPGRWPEAFVALAGPLSNIGLA
                     VVFGLLLRSIGAPSESFVTITAGIVYINVLLAVFNLMPVPPLDGSKLLFALFPDRIYK
                     LRSFFERYGLILVLFFILFVWQLIAPLVSIFFTFLTGLSI"
     gene            42819..43232
                     /gene="rpsL"
                     /locus_tag="PLD99_00265"
     CDS             42819..43232
                     /gene="rpsL"
                     /locus_tag="PLD99_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRH25372.1"
                     /translation="MSTINQLVKKSRNKPKRKSKAIALTRGFNTIKNRPVFFNSPFKR
                     GVCIKVTTKTPKKPNSAIRKIARVRLSNGQEVTAYIPGEGHNLQEHSVVMLRGGRVKD
                     LGIRYTIVRGVLDTQGVEKRRKGRSQYGNKRPKAK"
     gene            43236..43706
                     /gene="rpsG"
                     /locus_tag="PLD99_00270"
     CDS             43236..43706
                     /gene="rpsG"
                     /locus_tag="PLD99_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRH25373.1"
                     /translation="MRRKLKAKPKITPDSVYDSEKVAKFINYVMLEGKKTTARGVVYR
                     ALESVKELSKNPNPLEIFETALKNTGPTMEVRSRRVGGANYQIPREVRPERRQYLSMK
                     WIVDAARSKKGKPMHEKLAEEIIAASNNEGEAIKKRENTHKMAEANKAFAHLAW"
     gene            43777..43989
                     /locus_tag="PLD99_00275"
     CDS             43777..43989
                     /locus_tag="PLD99_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25374.1"
                     /translation="MILSGFSPNQQSMYGVRKQVRVIRVTGTVNDPWYTIKNPFGKKY
                     ILGVRDLHGVKVLGVFKRGGTKKPRA"
     assembly_gap    44146..44245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            44247..46415
                     /gene="fusA"
                     /locus_tag="PLD99_00280"
     CDS             44247..46415
                     /gene="fusA"
                     /locus_tag="PLD99_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRH25375.1"
                     /translation="MERDYPLERVRNFGIIAHIDAGKTTTSERVLFYTGAQHKIGEVH
                     EGETTTDWMEQERERGITITAAAITCFWGPTYLSESDRQKKEKKFRFNVIDTPGHIDF
                     TVEVKRSLRVLDGAVVVFDGVAGVEPQSETNWRYAEEAQVPRICFINKLDRTGASFER
                     SYKSILDRLSKKAVRFQIPIGEEDKHEGVIDLLKMKAYYFEGNMGNQVIEKDIPAEYM
                     EDATKYRGELVERIVENDEGLMNAYLEGKEISIEALKETMRKAVIGNKIFPVMTGSAL
                     KNKGVQLVLDAVVDYLPSPLDIPPVKGLNPNTNEEVLRHADDKEPFSALAFKLQADPF
                     VGQLTFFRVYSGTIESGSYIYNSTTGNKERLGRIVRLQANQREEVKKVFAGEIAAAVG
                     LKEAKTSHTFSDEANPIILDQITFPEPVISLRIEPKTKADQEKMGLALKRLSDEDPTF
                     RIKSDSETGETVIMGMGELHLEIIVDRMKREFSVEANVGKPQVAYKETITGSAEVENK
                     YIKQTGGKGQYGHVKITIKPLEALDPEAKIPKNVHREEGFEFVDSIKGGVIPQEFIPA
                     VEKGVREAMDRGILAGFKMTDISCELTYGSYHDVDSSEIAYKIAASQAFQEAAKKAKP
                     VILEPIMKVEVVVPEKFMGDITGNLSSKRAQIEAMEDRNELKVVRAKVPLSEMFGYVT
                     NLRSMTEGRGSSTMEFDHYAIVPKNVEADIIEKRTGAKAA"
     gene            46631..46852
                     /locus_tag="PLD99_00285"
     CDS             46631..46852
                     /locus_tag="PLD99_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRH25376.1"
                     /translation="MLVSERQKGVVKWFSDKKGFGFIGLRKGTPDVFVHYSSILGTGF
                     KTLPEGSKVAFDIERTKKGLSATNVRILS"
     gene            46931..47272
                     /locus_tag="PLD99_00290"
     CDS             46931..47272
                     /locus_tag="PLD99_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25377.1"
                     /translation="MYEDSSVLAENTPTSEEWKRLEEVFESGVAKDIILALKESGNRP
                     KDVALLNSRMRQGSLGGINSSLLRQGIPFRLEVVHNNCLFRENQVHLKRVLLPKPKIV
                     ETPRENSRRCC"
     gene            47502..48704
                     /gene="tuf"
                     /locus_tag="PLD99_00295"
     CDS             47502..48704
                     /gene="tuf"
                     /locus_tag="PLD99_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRH25378.1"
                     /translation="MADAFDRSKPHVNVGTIGHVDHGKTTLTAGILHVLDMNKDKGYG
                     ARVEAIDKIDSAPEERERGITIALHHSEYWTPARHYAHIDAPGHADYIKNMITGAAQM
                     DGAVLVVAATDGVMPQTREHILLAKQVGVPKIIVFLNKCDMVPDKELIDLVEEEIRDL
                     LTKHDFDGANAPVIRGSGLKALEAKDMSDEWAQKVFELISTLDSYFPNPVREMDKPFL
                     MPIEDIFSIEGRGTVVTGRIERGMVKVGEDAEIVGLRDTMKTTVTGIEMFNKQLQEGM
                     AGDNAGILLRGVKKEDLSRGQVIAKPGSVTPHTEFTAEVYILKKEEGGRHTPFFTGYK
                     PQFYIRTTDVTGEVTLNAGVEMIMPGDTATFNVKLTAPVALEDQTHFAIREGGKTVGA
                     GVVTKVVK"
     gene            48729..49091
                     /gene="rpsJ"
                     /locus_tag="PLD99_00300"
     CDS             48729..49091
                     /gene="rpsJ"
                     /locus_tag="PLD99_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRH25379.1"
                     /translation="MAKAKATTKKAVKAGATEGAGKLRIRVRAYESKILDVSVKQIID
                     TALRYDAEIQGPIPLPTEIKKYTVNRAAFVYKNAREQFEMRIHKRLVDIVNPSGKVIE
                     ALTNLSLPSGVNIDVKMI"
     gene            49119..49553
                     /gene="rplC"
                     /locus_tag="PLD99_00305"
     CDS             49119..49553
                     /gene="rplC"
                     /locus_tag="PLD99_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010541169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRH25380.1"
                     /translation="MKSIITKKVNMTQIFDESGRALPVTVLVAAEPIGEGALVEGDMV
                     VITGTSKGKGFQGVVKRHGFKGGRRSHGQKHSEREPGSIGGGARAGGRVAKGMRMAGR
                     MGNETVTQKNLKVVKILPESNEIFIHGAVPGRRGSKVMIKTN"
     gene            49555..50214
                     /gene="rplD"
                     /locus_tag="PLD99_00310"
     CDS             49555..50214
                     /gene="rplD"
                     /locus_tag="PLD99_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRH25381.1"
                     /translation="MEAQIYDNKGKEAGKIKLPEELFGLSWNADLVHQVATSLQSNMR
                     KNIAHTKTRGEVRGGGKKPWQQKGTGRARHGSTRSPIWVGGGVAHGPRNDKNFQRVVN
                     KSMRTKALYTILSRKFKEGEVLFVDSLNLSTPKTKDAVTLMKNLSKVKGFEKLENKRK
                     NAALFATATKSVGTEKSMRNIGSLEVVEARNLNPISLLSYKYLVIENPEASLKVLPGA
                     K"
     gene            50219..50548
                     /gene="rplW"
                     /locus_tag="PLD99_00315"
     CDS             50219..50548
                     /gene="rplW"
                     /locus_tag="PLD99_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRH25382.1"
                     /translation="MAEKKTTKKEKKVVEKAEVSKSVIVSPRMTEKSVLSQESKGVYV
                     FNVKRDSTKKQIAASVKADYKVTPLKVRTSQVPSKQKFSRGKWGVKGGGKKAYVYLKK
                     GDKIAVA"
     gene            50557..>51263
                     /gene="rplB"
                     /locus_tag="PLD99_00320"
     CDS             50557..>51263
                     /gene="rplB"
                     /locus_tag="PLD99_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRH25383.1"
                     /translation="MKRYKPTTKSRRNMTGINFRKLITSTTPVKSLTTGFKRGDGRNN
                     TGRITTRHKGGGHKRRFREIDFVFDKKNVPFTVKTIEYDPNRSGFIGLVAYKDGERRY
                     ILLSKTMRVGDTFVASETATITPGNRLPLKNIPVGTFVYNVELKPNGGAKIARSAGNY
                     VEVSAKDAGYVDLKMPSSEVRKVLETAWATIGEVSNDENRLVNLGKAGRSRWLGIRPT
                     TRGTAMNPVDHPHGGGEG"
     assembly_gap    51264..51363
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <51364..51941
                     /locus_tag="PLD99_00325"
     CDS             <51364..51941
                     /locus_tag="PLD99_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRH25384.1"
                     /translation="NLVPCPLRKRAFLFAIFIIKRYISDMKQLDPRAVWLFFLSGGTR
                     ILVFLLVAFSLIIQAYFEYKDAGEAVPLLLRLWWLIPILCMIALIIYAKLSYSFYKYE
                     LTDMGFKKESGIIWKQYVTIPYDRIQNVDIYRGIFARILGLSDLQIHTAGVGGVAMGE
                     GRLPGLSVSDAEKMRDELLVRAKQSRNGQGL"
     gene            52014..52397
                     /locus_tag="PLD99_00330"
     CDS             52014..52397
                     /locus_tag="PLD99_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25385.1"
                     /translation="MSKRHGTARPVGSFREGDVVSGKMIIIFATTRNTDLTEGRGTTI
                     DHSYHLSARDAEVGAHGIDVMGSDGGIREVLALQEDQSGRVYLFANNASPVTIKENSE
                     MVANLRKRALSKLSSSERAALGLDD"
     gene            52853..53338
                     /gene="rplJ"
                     /locus_tag="PLD99_00335"
     CDS             52853..53338
                     /gene="rplJ"
                     /locus_tag="PLD99_00335"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRH25386.1"
                     /translation="MAISKQKKGEIIDKLSKAIKDAKSLVFVNFHGLKVSDTTNLRRE
                     LRKEGVEYQVSKKTLTKRVLDTEKFEGTMPELEGELAMAWSEDLIAPARGVHAFAKKF
                     PEALKILGGVFEGKYMSKAEMMEIATIPPVEVLRGKFVNIINSPIQRFAIALGEVAKK
                     K"
     gene            53343..>53677
                     /gene="rplL"
                     /locus_tag="PLD99_00340"
     CDS             53343..>53677
                     /gene="rplL"
                     /locus_tag="PLD99_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRH25387.1"
                     /translation="MTQEQIIEAVEKLSVLELNALVKAIEEKWGVSASAVAAGPAAGA
                     APAEEAKSTADVELTDGGANKIAVIKVVKEVLALGLKEAKDMVDAAPSMLKAGMKKED
                     AEALKKKIE"
     assembly_gap    53678..53777
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <53778..54373
                     /locus_tag="PLD99_00345"
     CDS             <53778..54373
                     /locus_tag="PLD99_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25388.1"
                     /translation="VGVKYAHMGKSISHLFGSEAKVKVMRLFIFNKGAIFTAKTISER
                     VQASAKVIRKELASLIKAGLIYKRARGYVLNAEYDYLKAVERFLIEATPISEKEIIRK
                     LSKAGSMRLILTSGVFKQDSEARVDLLVVGDKIKEGRLLKAISSLEADLGKELRYAAF
                     ETPDFKYRLGIYDKLVRDILDYPHRKLVDKVGLQPGV"
     gene            54426..55118
                     /locus_tag="PLD99_00350"
     CDS             54426..55118
                     /locus_tag="PLD99_00350"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25389.1"
                     /translation="MYISNWGNVLTLSFQNLWMGIVSFVPNLVVALIIVILGWLVGAL
                     LGRAIWQVFRSLKVDEALRRAGFEGFLRRGGVDLDSGAFIGGLVKWFVVVVFLVAAFD
                     VLGLTQVNLFLQQVVIAYIPRVIVAALVILVGGVIGDVVERVVVTAARTAEIRSAHFA
                     GAVSKWAVWVFAILVALSQLGIATAFSQTLFTGIVIAISLALGLSFGLGGQEAAARFI
                     ERLRGDMGGKHN"
BASE COUNT        14837 a        11566 c        13042 g        15172 t
ORIGIN      
        1 ccaaccgatc gatttcctgc cagcccaacc aacagtaccc gcagttgctc ctccagcgaa
       61 gcccattgcc cacttaccga gtttattgat attgccgtct gcttgatccg ctacaccctt
      121 agatatggtg aatgatgcta ttaggaagat gatgatcatc acatagttga ccagggtagg
      181 cacagcccct acagtgaagc tcgtactaga gagtgcgtta ccaagtgatt cactggtcat
      241 tccggtaaag actgtcgcgc taattccttg tatcaaaata actgttaccc aagtcataag
      301 catgaagaca ggtgcgaaga ccgcctgccc tattagagtg gacttccact tacttgcata
      361 tccactgagt cctgggaaaa ccattgagag gaacatgatt ggagagagca caagtacgaa
      421 tatgagcgct acataacgaa cgataaacat cactgccata gcagagaaga tgaaggtggt
      481 gacaatgagc actatgattg tcatcacagc actagtaagc actactgaac tgtcgatgct
      541 accgtacatt tgccacaact tttgggtacc caatgcgtct atgatgccgt ttgaaagtcc
      601 agcgttgagg aagttctgag gtcctaggag tgcccctgga gctatagctt tgtagaaggt
      661 cagagcaaga aggttagtca catcgataat caccttggtg aagaagaggc tgaagttgac
      721 caacacagcc gctataacca tcttcactat taaccccttg gtatctttgt ctctaccaac
      781 tattgtcatt attgaggagt acaagagcat gaagatgaac cccatgttgg ccacatctct
      841 tataacggtc caggttatgt caattgcagg aagtctacca atgttctctg acatggctat
      901 aaccgtgtag taaatagatg cgttaaggac gactcccgaa agcatagtga tccaagagac
      961 tactttgacc aaggcgatca ctaatggaga gagaatgacg ccaacgaagt caaagctccc
     1021 taaaaccatt tttgccgccc cagcagccag ccagttagtg gttgtttgtt cttgtgccag
     1081 tacataacta gttggcacca caagaaaggc taggacgatg attgttagca ctatgatatg
     1141 tggtaaaaac tttctcatat gcctgctgag ttatttctag ctgaatctgt gacacttaca
     1201 gatccattgc atgatatgga cactgcttgg ccagaagggt ttgtggctag gatattcgct
     1261 gtctttgtac cgacaaagct gtacctaact tgtaatggct cgacgaagaa tgtgtccgac
     1321 gaggctatct catcgccaaa ccatctgtag gttgttcctg ttgggtaact acttgtcatg
     1381 atccactcca tgtcagtccc tacttgtgta gagcttgttg atggtgcgca agatagagat
     1441 gggttacttg ttgtaggagt ctgagttgtt ggtgccgggg tattcgttgt tccgccattc
     1501 cccacctcgt ccccttcgaa tgtcggtgcg ggcacctgat ttattgcatt gatgtttgga
     1561 tcctcacatt ggccaattct atctttgatt cctctcgagt actgaagagt tttgagagtg
     1621 ttgtttctaa tttttattag ctcgttaaca tctgattgac caccgaactt caagtcatcg
     1681 tcttggagta atgagctaat gcgtccacca atatatttcg agtattggct taaggtaaag
     1741 accaccgggt cccatgccat cacttcgtat ctagatattg tactgactaa gtcgtcgaca
     1801 gctgtgttca tggtggtcat tctgttgctc caactgaccg agttctcctt tctaaagtcg
     1861 tttctagtta tgaagtcatt gaatacttcg agcgccttaa tggtgcctgg aatctcctta
     1921 cagattggta tgaagtatgg agagttagag acgctgccag acatgagaca gttgtcgttg
     1981 cttggatctg agtttggatc tttctgcccg tgatgacaaa tgtcgagtat cccgtcttcg
     2041 ttgtagtcat atccgtctgg aatgttgtcg ccgtccacat cgatgatttc attcctggtg
     2101 tctgcaatag aatttttgtt gaacagtccc ttgcctttgt cgttaaacac atgtctttgg
     2161 agtagaccag caagtagagc actcgctaga gagtctatat cgtctgctga tattagtttg
     2221 tctactcctg agccaagagc tttgttgagt tggttagata ttactgatcc cggtgtggta
     2281 acctcttcct caagacaagc agtcggttcc ttatttgggt tccaattggg atactcattt
     2341 aaaatattct gtgcttcctc tgggtcagca tcgtagtatc tagccatggt ggccatatta
     2401 ggaggttggt ttttcttcaa gcaggttgtt ttgctcatga atccagtgtt gagcaccctg
     2461 tcttctctac tgactgagag tgtgtttgcc aacctgctat ctatatcgag ttttgccttt
     2521 aggaaagcgc cttgaggatt attttggtcc tcttgggtca ttttgagcca agcgtcccaa
     2581 cctccctggt taaagtcttg gtaaaagtta tctatgttta ccccaatttc acttagagta
     2641 cattggtagt ttgatgtatt ttgggtgtat tgcttgacca aagataatct aatttgtgct
     2701 tgaaagggcg aacaaagaaa gccgaaagaa gtatcgttaa ttgcatctcc caccacccca
     2761 tttgcaatgt ttccaaaata acttcctgta tcagttatga aagcggggtt accatcaaat
     2821 ccagtattgg cccatttgat tgttgattgg acgatatcgt caatcatttt ttgagcaagc
     2881 tgcttaccaa ccttgtttac caagaaatca ataatggtcg gaacgctctc aaggtttatg
     2941 tctcctactc cccaaaaagc ttgggcttta ggggcagcga ttggagagaa gaaaatagaa
     3001 aacccaaaga caaaaaccat tacaagggat aggagtcttt ttctaaaagt tttagttttg
     3061 gattccattg tctaagagta ctttgattat gtcttctact atcctctctc cgccacctat
     3121 gttatttctt gcactaatta caccagcgaa agacagcgca gagtcgacac tctgatctga
     3181 aattgatttc atgcctgagg atattgagag atagttgttt actaggtcag tgtgtaagac
     3241 taaaagtgag gatggtacct caatgttttt taattcttga gcagcagttt cgtatgcgag
     3301 agaaattttt ttagcagcat cctccagtga cttgtctgtt gagtttactt ttgttgggtc
     3361 actaccgatt gaactaattt tatttatacg tcttctttct atctctgttg ttatgaggtc
     3421 gtacttttga aaattggctt tagcattgct caccacattg atgtctaggg cacttacttt
     3481 tggcgctgtt tttatatcgg caatagagtc agcatatttg tctgctagtg ctgtaatatt
     3541 ttcctcagat atttggccat ttgtggcgag attaatgtag tcggttataa gctgtctacc
     3601 cactagatca gtggtggtta ggttctctga atcgtctgta tcccgctcag tactaaggtc
     3661 ggaaactgag ttaggctcag gaacttgata gttattattt atttcagggg tttctgccga
     3721 tactttgaag acgcttttct ctttatctgc tctttgccat gaggcaccga gtactagtag
     3781 gaatgaaatg aaaagtgcta gcgtgacacg tgttcgcatg ttcctcttat tatatcagag
     3841 gtatacagac aaaattatct ttatatataa gtagtggata ctgaaacctt aataaatttc
     3901 ttattaaggt ttattagagt ttgaggctaa gctccttcca taaatctaga ggtacatctt
     3961 cggctctggt gttctcactt aaggaaagat ttttgaaagt ttccagaact ttatctttat
     4021 cgaagacgac accgaggttt gaagacaatt tcttcctctt tgaggcgaat ccagcccgca
     4081 aaatcttgaa aaaatgcgcc tcttccacac tattttgctc aaaaaaggtc ttagagatgt
     4141 tgtatatggc caaaatggcg gagtctacct tgggtgctgg gctgaatgag ccagccttga
     4201 ccgtgtccac tagctttggc tcaccataga tctttattga tattgagagg atgctctctt
     4261 tgctgtttcg agcgataatc ctctcggcaa cctccttttg taccagaact accattaatt
     4321 ctggttgatt tgagttcgag aggaacttct ctagtatggc accagtgatg ttgtacggta
     4381 tgttggctat gagtttgtat ctatcccact taggagtgag gtccaggata tcttcgttca
     4441 taagagttag ttggccagac tcgatctcgg tagcaaacat ctcttttagt ttgagggtta
     4501 aatcatcatc cttttctact ccgatgacct tggcaccaga gtataagagc catgaggtta
     4561 gagcgcccag tcctgggcca atttctaaaa cagtgtcaga gggtgagagt cttcccgcct
     4621 ctacaatctg gtttaaagct ttctctgact tgaggaagtt ctggcctaga gatttctttg
     4681 cgtacatgag tctatagtga gcttagttgg tctagaagtc tatcttgtac aaaatgtctc
     4741 ttcgggtatc tcgtcttggt actgagccct cgttccatac ctcttcttcg gtaagttcct
     4801 caggtacatc gaagatgaac tcggatggga ggtttgtctc tctttggcca aacactgttc
     4861 taacttgtgc gttagttaga aaaagttttt ttcttgctcg cgtcaccgca acgtagaaga
     4921 gtctcctctc ctcctctctc tcctccgcgg tgatattctc tcgagtaatt ttgttgtggg
     4981 ggaagagtcc gtcttcgagt cccgagatga agacgacatt gaactcgaga cccttagatg
     5041 catggatggt catgagtctc actccattct tattttcttt ttcgtttttc tttctatcta
     5101 aatcatcttg gtcgctctgg agcgctgcat cctttaggaa gttctctatc ccctcacctt
     5161 ggggtaggtg gtcatagcgc gaggcgaagg ctacaagctc gaaggcgttc tctagcctat
     5221 cttgatcttc gtctctgcct ccgcgccact cggcttcgag tcctgtgctc tttataatat
     5281 gatttataac ttcgcttggt tttagttcgt tggccttctc tcctatatct gctagcaatt
     5341 ttctaaagtc agcgatcttt acccttgtac ctgctggtag agtagtctca tctcctgcaa
     5401 aaattttagc cacagtggtc ttgccgatac ctcgagtagg agtgttgatg atcctcttca
     5461 catctgccag tgcgcccggg ttaagtcctg cacgaagata ggagataatg tctttcacct
     5521 cgcgtctctc gaagaatcgc gtgcccagta gctgataagg aacctggtgc cggagaaacg
     5581 cttcttcgag cactcttgat tggaagttcg ctctataaag tactgcaatc tcatctgcac
     5641 tttcccctcc tgctatcaaa cttttggcct tagtagcgat gaattcggct tcagagatct
     5701 catcgaaggt tgagagtagg ctgatcttct ccccgctctc actctgggta aaaagatttt
     5761 tctccactct aattttgttc ttcttaataa tagtgttcgc cgcctctagg atggtttttg
     5821 ttgagcgata attttcttca agaagaatta tttttgtctc tgggtaatct ttctcaaact
     5881 tcaaaatgtt ttggatgtct gcccctctcc aagagtaaat gttttgatct atgtcaccca
     5941 ccaccgtgat attcttctcc tcgcttgtta agagcttcgc tatctcgtac tgcactttgt
     6001 tggtgtcttg gtactcatct atatgtatgt acttccacac ctgtcgatag taatctcgaa
     6061 cctttgggtt accacgaaga aggcgaacag tcttcaaaag taggtcgtcg aagtcgagtg
     6121 cgctgtcgcg cttgaggatc ttctcgtact ccccccacac agacttaact gttgatgcga
     6181 agtggccacc ctcctcgtgt gacatgtatt cctcagaggt agtgtcgcga cccttctcct
     6241 ttgagatgat ggagaggatg cggtctggtt catgggtctt ggggtcgaat cctgtgttaa
     6301 ccaaggcctc tttgatagcg cgcttcgcat cgtccttgtc aaagatggtg aagtgtctgg
     6361 atatgcctag ggtctggctg ttctccttca atatatgaac tccgagagaa tggaaggtgg
     6421 atacaaaggg cttctcggtc atggttatgg ggcgactaag ctctgtgtcc tcaaggagca
     6481 tcttctctac cctctctcgc atctctttag ccgctttgtt tgtgaaggta atggccaaaa
     6541 tggatgacgg agtcaccccg tgctttacta aattccttat tctctccgct attgtcttgg
     6601 ttttgcccgc cccggcacct gcaagtataa ggaggggccc ctctagggta tccactgcct
     6661 gttgctgtct ttcgtttaga gtagccataa tatatgcgaa taatagcacg atttactaaa
     6721 tatggctcaa tttagccctt tttgctctat actagaacac aagaatccgc tctagaccct
     6781 tcatccgacc ctcattatct ggtataatag atacctaccg aaacaggagc cacgacagct
     6841 gtcagatcta aaagtttaac caatcgttta ttactcaggc aaaggattct aatattgttt
     6901 ttctaaacaa gagggttatc ctgctactct tgcttgtttt agccggtacg atcaacctta
     6961 gtacgaccga agctcagggc gagaaggtcg ccagagctcc acaagttgta agatcagcca
     7021 attctcagaa tattgagctt cttaagggct cttctaatat aaacccgcat ccaagccagg
     7081 gtacttatct tgctgtagta gacaatagtg ctcttgaggc taaggcaact cctgacccta
     7141 caacctatgc cgaccaaggt atctctggct caggacaaat ttctgtatat atagtgcgag
     7201 atggtgacac tttgtcgacc atcgccaaga tgttcaaggt ttctatcaat actatcgtct
     7261 gggcaaacga cattaagggc ggtcttatca agccaggcca aaccctcgtt attctcccta
     7321 tatcaggggt aaagcaccta gtggccaagg gtgacactat cgagagtatc gccaagaagt
     7381 ataaggccga tgtagccgaa atactttcat acaatgattt gaccaaactt tccaagctct
     7441 cagccggtga cgagatcatc atccccgacg gtgagatcac ctcagttgtt acctattctt
     7501 ctggttctgg cacagcgaat actacagtca cctcaggtta ctttgcccga ccagtccagg
     7561 gtggtaagag aactcagggt ctacatggtc ataacggtgt cgacattggt ggtcctatgg
     7621 gcactccagt acttgcctca gctggcggta ctgtcatcat cgcaagatct agtggataca
     7681 acggaggtta tggtctatat atagtagtta gtcatccaaa tggtactcag actctctatg
     7741 ctcacctctc ggcagtaaat gtctcagttg gcgacaaggt aaccaagggt caggtaatag
     7801 gtaagcttgg taactcaggt aagtctactg gtccacacct ccacttcgaa gttcgcggtg
     7861 ccaagaatcc gttctagttt tataaatcaa acttttttgg gcgatactcg acagcttcga
     7921 ggatgtgctc acgcttgatc tcatcacttt catctaagtc agcaatggtg cgagctacct
     7981 tgagcatcct gtgatacaca cggggtgaga agttgagtct ctcggcggcc atgtttaaag
     8041 tttgtcttgc ctcattactt agtggtacaa agttgataag gtctcgtgca ctcatctcgg
     8101 cgttcttggt tatcttactc ttctcaccaa atctttttgc ttgtgttttc ctcgcatctg
     8161 caactttgct tctatataca tgactcttag caccctctgt ctcgcccgat aaatcatttg
     8221 gcaatactcg atcaacctcg agccataagt cgattcggtc tactattggg cctgacatct
     8281 tgcgtctata tctttgtagt gcacttggag tacagacaca ttgcttgcct ctgaagccaa
     8341 agttcccaca aggacagggg ttcattgcag cgacgagtat cacatctgcg gggaatctgg
     8401 ctgtaccctt ggctcttgca actgagatca ctttgtcctc tagtggttgt cttaagctct
     8461 ctatcacccg cctctcgaac tcagggaact catccataaa gagcactcca cggtgtgcga
     8521 gcgtaacctc tccaggttta ggtgttgctc cgcctccaac cagcgaaaca taggaacttg
     8581 tgtgatgagg agaacgaaag ggtggattaa caataaattg tttgagcact cctgcaacag
     8641 agtgtataga tgtagcctct agagcctcgt cgaatgtaag aggtgggagt atggaggcga
     8701 aggccttggc aagcatggtc ttgcccgtac ctggtgggcc gtacattgcc acattgtgtc
     8761 caccagccgc agcgataagg agtccgcgct tcgccctttc ttgtccacgc acatcactaa
     8821 agtcggtgtg cgattcttca tcacttttcc caaaaaatgt tttgggttgt tgtttgatta
     8881 gattcctacc ttctaggtgt gacaaaagtt cttttaaact ctctactcca aaaactttta
     8941 taccatcaac gagtgcagct tctttggcat tgtctttggg tacatatatt tcttctatac
     9001 cctcgtcttt agcgaactta gatatcgaga gcactccgcg agttggtctt agtgtgccgt
     9061 cgagtgagag ctctcctaaa aatattttat tcttcgtatc aaaatgcact tcctcactcg
     9121 caagtaggta cgagagtgcg ataggaaggt caaatatggc accttctttc ttctcgcttg
     9181 cgggagcgag agagatcacg atcttttggt tcttctgctt aggacttgtt aaaccagagt
     9241 tcttgattgc agcacccact cggtccttgg cttcttctac tgcctggtca ggaataccaa
     9301 taatagagaa agcatacaaa ccgcgggaga ggtcggtttc tatctcaact aatgagacgg
     9361 atagtgctgt agtctgagca ctaaaaaccc ttgatacaga catacactaa ttgtatatca
     9421 gcggaagggt aattaaatag cagtcttaga cactctctat atgagtcttg caggtaggag
     9481 cggctgggtt cgcctctagt ctgtcggctt ctatatcgtt accacatact ttgcatacac
     9541 cgtattctcc tgactcgatc ttctctagtg cggcgacgac atccttcagc ctgccctcta
     9601 gtgagtttgt tatagccatg ttctcttcga agtcctctat agcgtcggcg gcctcgttct
     9661 catcagctga agcattctct ctctcaccta aagctgggtc ccagttggtg ggatggtttg
     9721 gatccttctc agctacggtt gcaagttcgc cttcgatctt tttcttctcc tcctctaact
     9781 ttgttttgta ttggtctgtg tttgaaatca tatgggaatt gtatcaaagc ctgggctttc
     9841 gtctaccttg agagtttctc tgcctggagg cggttgataa tggtgcggaa ggagttttcg
     9901 ttgtcggtca ttatcaaagt atcattgtcg aagaagccgt atagtaggac tatttggttg
     9961 tacgagttcc taagcaccct agtatcgtgg ttctgtatcg tcttgtcctt gaacactgag
    10021 ttcgtggcta caccatcgag tagagccggg tcaataaata gaggtaacat atcctcgacc
    10081 agtctactct cccactcgag catgccgggg aaggtgttct caaaagattt tagtttaaca
    10141 attagaactg agtgacttgg gctcgagccc aaaataccga acataaagag tggatcaaat
    10201 gctctgacga gtgggttagg tgcatgcgag ttcaaacgag taaggaagtc agagaatgcc
    10261 aagagttctt tgtttggttc cactcctcta ctgaactgaa tctgatctag agtgtccccg
    10321 cccctcgact cactgcggac tttttgtacc tcatctatga cagcttgtct gccgagagtt
    10381 gaggcatcta cttcgatggt tttatctact cctagaaatt ggttcttggg tgcattaact
    10441 actggcaagg ctgttttctc ttgataagtt gtgagtgcat agtacgcgcc gtacccacca
    10501 ccactgataa agatgagggt aaagagcgcc ataacaagtg gtttgaagga gaagtgactc
    10561 tcgctctgtc ctgcatttgg agagagtgtt tgtatggctt ccctcttttt ttcctccttt
    10621 ctttgtatgg atatgagaga ctcgttctgt ttccttattg catccgcaac atcgccctcg
    10681 aaggtgcgaa tttgtttcaa gtgagtccct tctggttctt gaggaggaac attgttttgt
    10741 gcaatcgctt ctgcaattgg gtttggtggt actgcttggg gtgccggtgc tggtgctaat
    10801 ggttgagaca cttgtgctgt tgtttgtggt gcgaccttgg gtacgaaagt gatttcggga
    10861 ggggtctctc cactagactt gatttggaag gagccttctg gtggcatgac tctattctaa
    10921 cagagctcta tattaatagg gattactcgg cagtttcttt aggtgcttcc ttaggagctt
    10981 taacttgaga ttgtctcctg ttttgcattc gtgtttctac gcccaggagg tactttttag
    11041 ggttgctgtc taggtcgagg aagtcgtccg ctgcttcgat aagcttagaa gatgatgact
    11101 tgccgaatga aacaacttct acctgagtac catgagtgtg ctttagataa tcaatgagtg
    11161 aaacgaagtc accgtcgccc gaggctatga tcactgtgtc taatttgtta gcgagtttaa
    11221 cagcatccat agctaggtcg atgtcccaat tcgctttctt agcaccaccg gcaaatattt
    11281 gtaatggctt ggtgcgtgtc tcgataccaa ctttagatag tgcgtcgaag aatgccttct
    11341 catctccacc ttcggtggtg gctacataag cgatggcgcg aacgaggact ctattatcta
    11401 gagcttcttt gatgatggca ccgaagttcg ccttagcgtg gtacaggttc ttggcagagt
    11461 gataaaggtt ttgtgtatcg atgaagatgc ctactctctg ggccttgtgt ttaataactg
    11521 acatattgtg taaatctatt taataaatag atttcaaaga ttttacttct tcaaactgag
    11581 tttcttcact agagcgctgt ggcgcttagg atgcttcttc tccaaatatt tcatatgagc
    11641 ttggcgacta gcgacaagtc caagtagtcc gcgtcgagaa tgattatctt tatgattctt
    11701 cttcaagtga gaagcaagct cctcgatgcg agctgtaagt atggaaattt gtgcctcagg
    11761 agagccagtg tccttgtcgt ggactctagc ctctttgata acacgtgctt tttttgtctt
    11821 tgaaatcacg tatctataat agcacacctc taccccctta gcaagagctt aannnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgatgcaa atgctataat gtcgcaaaac
    12001 atggcaagca cttcagaact agagaatttg aagagacagt tcctagaaca taccgagatc
    12061 actcgtggca ggagtttgaa gactgtagag aactacgata gatacttaac gcgtttcatc
    12121 gagttcgcta aggtgaagtc gccgaaacat attaccgact ctgtaatacg agagtaccga
    12181 ttgtggttaa acagacaacc aataaccaag aataaaaaaa atcaggatac gctaaagaag
    12241 cgcacacaga attattatat gatcgcgctc cgcgcgtttc taaaatatct cgcatcacaa
    12301 aatatctcta ccctctcccc cgagcgtatc gaactcgcca aagtttctga aaggcatttg
    12361 gatctaatca ctcttaacga actcgagagg ttgatgaacg cacctgacac atcgacactc
    12421 caaggtctcc gcgacaaggc gatgctcgaa ctctttttct ctactggtct tcgtgtgtct
    12481 gagcttgcat cactttctcg cgacttggac attgatgccg atgaattttc gataagaggt
    12541 aagggagaga aggtgcgagt tgttttcatc tcaccacacg cgcgtgatgc ggtgaaactg
    12601 tatttgaaga agagaaacga cttggaagac gcaatgtttg tgaatattgt gaaggatcac
    12661 accaagactg ctcccactag attaaccact cgctcaatag agaggatagt gaaacgctat
    12721 gctatcaagg ccggaatatc taaaaaggtt acaccgcacg tcattcgtca ctcatttgca
    12781 acggacctac tgagcaatgg ggccgacatc cgctctgttc agatgctcct tggtcacgcc
    12841 aatatcgcca ccacccagat ctacacccat gtgaccgata gagcgcttcg ggaaacacac
    12901 aaaaagtttc actccaagag gaagtagtta tttgacagtc tttatataaa gagtagattg
    12961 gctcgtgtag cgaaccttcg ttttcttcca ccatgaggct tcaaatggac ttcattgttc
    13021 ggaactggct gatctgggct gtggttgcca tcgtctgcta tgtcgttggc atgatcaacc
    13081 agatgagttt gatggtgcga atggcaagac gcggtcctct gtcggggaat gtgttcaagg
    13141 ggaccggagt cacctttctc ttcttcggca tcggtggcgt ttccactttc cttctcgttc
    13201 tttctgtggt cgtttcgctg atcgacagat ttcgttccta accacctcca aatttatgga
    13261 ggtttttcat ttgacaaagt aggcagagtg tgtatataat agagtgtgta agtaatagtg
    13321 ctgtttatgg gacccgcctt tggcgggatt aatttttaaa aaatatgttc gatcttaaag
    13381 tcatcaacag tgttctcggc gagctagaag aagtgcgcgg tattcctcgt gccaaaatta
    13441 tcgaggctat cgaagcggct ctagccactg cttataagaa ggagtacggc aagcgaggac
    13501 aactcatccg cgcgtctttc gacatgaaga ccggtcaggt tgagttcttc caagtgaaga
    13561 atgttgttga tgatactaag gtcttcttcg acgaagaggc agaactagcc gaaggtgacg
    13621 aaagagtttt ctacaaccca gaacagcaca tgctcctaga tgatgcaaag aagatgaagc
    13681 gtgatgctaa ggttgatgac gagatcatct tccctatcga gtcgaagggt gactacggtc
    13741 gtatcgccgc tcaaactgcg aagcaggtta ttatgcaaaa gattcgtgaa gcagaaaaag
    13801 tttctgtcct tggtgagttc ggtgcacgcg agggcgagat tgtctctggt actgtacaaa
    13861 gaattgaacg cggaaatatt ttcgtcgaca tgggtcgtgc tacaggactt cttgcatatg
    13921 aagagcagat ccctggcgaa cgcttctcac aaggtgagag agttagagca tatctatata
    13981 gagtagaaga gtctccaaga ggtgtcttcc tacgtctttc tcgctctcat ccaaagttcc
    14041 tagaaaagct ttttgaagct gaggctccag agcttgcaaa tggcgcagtg gttattaagt
    14101 ctgtcgctcg tgaggctggt taccgttcta agatcgctgc tcaagcactc gactcacata
    14161 tcgatcctgt cggcgcactc gttggtcaac gtggagtaag agtgtctaca gtgatgtcgg
    14221 aacttcgagg tgagaagatc gatgttatcg agtggcaaga agagccaaag aagtttattg
    14281 aagaggctct ctcccctgcc aagatcatga agatcgaaat ggatgaagag aataaaactg
    14341 caattgtgca tgtcgctgag tcagagcagt cactcgctat tggtaagggt ggacaaaatg
    14401 ttcgtctcgc agccaagctc actggattca agattgatat tcaatctgct aagggtgagg
    14461 aacttgcaac aggagaagca acagctgata ataaagagta attatgaatc tttggcacga
    14521 aatatctcca ggcacagccg atgagatgaa tgtaattgtt gagattaaca agggttccaa
    14581 gaataagtac gagatagaca aagagacagg tctaattgcg cttgatcgtg tgatgcacac
    14641 ctctcaagac tacccttttg actacgggtt tatacctcag tcactttggc atgatggaga
    14701 ccctttagac gtggttcttc tgaccaccta ccctctttct ccaggaatac ttgttaagtc
    14761 tcgtccagta gctgtcatgc acatgataga tggtggtgag gccgatgaga aggttattgc
    14821 cgtacctgtg gaggatccac gctttgacga cattaaagac cttagcgata tcaacaagca
    14881 tactctaaag gagatccagc acttcttctc tacttataag aagctacaga acaaggaggt
    14941 ccaagttggg gatattgagg gtaaggaggt ggcaataaag gctttcgagg agagtttgga
    15001 gctttatagt agaaaattca gcaagaatta gacgtttttc tgggtgtgat ataatcagta
    15061 tccacggata cggtcgaatt tactggttac ttaaatttgt aatgaagaaa ttaagcattg
    15121 ttcttagcct tcttttagca attgtcgcta ttcctgctct tgcagaggat ggtcagacta
    15181 gtggtcgagg agttagagac gacttcgagg ctaaaaaggc cgaaattcaa ggaaaaatca
    15241 aggaaaataa ggataaaatg aaggaagaga gagagttgtt gaagggggag attaaggata
    15301 agcgtgagga aatgaaggaa gacagaaagg agttaagaga tgacagacaa gaggatagac
    15361 aagataaaag agaggataga agagaagaca ggcaagataa aagagaggat aggagagagg
    15421 atcgaggtga gaagtctgta aaggttaggg ctgatgttgc tgtccgagtg ctagaggcaa
    15481 ccattactag actggagaca cttgcgacta gaattgattc tagaatagcc aagattggtt
    15541 caacaggtac aagtactact gaggctacag gttttgttgc ttcagctagg actgatatcg
    15601 gtactgccaa gactcatata gcaaatatca agggcattga tctttctagc gacactgcta
    15661 gttcaaccgc cacaagtaca aacaaagtta atcgagacaa gatcaagtca gaggcaaagc
    15721 ttgctaaaga agctcttgtg agtgcaaagc agaatcttat gaaggctcta aacgccataa
    15781 agagaatcga ggcatcacta agaaaggaag gcaagttgcc ggtggctaca tcaactgcta
    15841 ctactacgac tccttaatag agataagtaa ttcacaatgg aaccaaatca aaacaatcaa
    15901 ccagttcagt cgaataatga aaactcaatt ggtcctgtta tagcgatcat cattgtccta
    15961 ctcatggtga ttttgggcgg agtctacttc tggggcgaga gaaataactc agcacaacca
    16021 gcttctcaag aagttgttgt aaatgaggca aatgtagatg cagtatcggg caagattatg
    16081 caacaatcaa cttccgacga gagtgcatcc atcgaggaag atctcaatgg aacagatgtt
    16141 gatacagtcg gggcagatct aaacaatctc taaagtttat tagaagagca aaaaaacagt
    16201 cacgatagct tcgggactgt ttttttgtta gactcggaga catgtcaaaa cacgagcata
    16261 aacataccaa caatctaaag atagatagga tcaatggcga ggcgcatatt acgggtgaaa
    16321 ttacactaga agcgttagaa gaggccagag gtgaggctat aaagtctctt aattctcgtc
    16381 tagaaattcc tggttttcgt aaagggttta ttccagacaa cgtgcttgtg aagaatattg
    16441 gagagatgaa ggtgctagag gaggttgcag aaatcgtact tgcacatgag tatgtgcaca
    16501 ttacgagtga gaacaagctc tcccctatcg ctcgtcctca gattgctatt accaagctcg
    16561 ctcctggtat cccacttgag ttcaaaatta cagtagccct agaacctgag ttcactctcc
    16621 ctgattacaa aaagattgcc aaggattcta ggaaggaaga cgaagacttg aaggtagatg
    16681 ataaggaggt tgatgcagtt ttagccgaga tagagaaaag aggatggaag ccagagctta
    16741 aagaaggtga ggatctccgt actaaagcga aggagaatct tttagaagag aaaaagttta
    16801 gagccaagga gaaggtcaga ctaactattg tagagagtct agtgaaggcg actgacatta
    16861 atattcctaa cgtgcttata caaaatgagt tagatcgcat gattgcgcag ttcaaggatg
    16921 atgtcgtaaa gcacggaatg gtttgggatg aatatttgaa gtcgattaag aagactgagg
    16981 tagatataag atcagaatgg aatgacaagg cgctcgcgag ggctaaggcc gagctcatca
    17041 tgcagaagat cgctgagact gagaagctcg tgcctactaa ggaagaggta gagcacgaga
    17101 ctactcacct actcactcac tacaaggacg ctgatcccct ccgtctccgt atttatgtct
    17161 tcgagcaact aaaaaaccaa aaagttttcg aatttcttga aacaatctag gttttgttat
    17221 agtgtattgg aactatgagt tacctagtcc caacagttat agagaagagc cagtttggtg
    17281 agagagcgta tgacatttac tctcgactac tccgcgagcg cattatcttc cttggaggtc
    17341 ctattgatga tgcggtagca aacactgtta tagctcaact cctctttcta gaatcagaag
    17401 acccaaagaa ggatattact atatatataa actctcctgg cggtcagatt cagtctggtt
    17461 tggccattgt cgatgccatg aaccacatca agcctgatgt ctctactgtg tgcgtaggta
    17521 tggctgcttc gatgggagca acgattctat ctcaaggcaa gaaaggtaaa cgctttgcgc
    17581 taccaaatgc agaggttatg atccatcaac cgcttactgg tgttgagggt caagcatcgg
    17641 atgttgagat aactgccaag cacatcatta gactcaagga taaactagcc aagatgatgg
    17701 ccgatgttac aggacagaac tacgctaagg tggctaagga taaggacaga gactattgga
    17761 tgactgcaga tgaagctaag aagtacggca ttatagacaa aatcctatag ctctggtact
    17821 ctctaactgt tcaatctaaa tttgttttaa ctgaaacgtg cttgtttgat tacggatacg
    17881 actgaacaca ccccatatgt ctctgaaatt ggccctatta ttgatgctac ttgcattagc
    17941 tgccggcacc ggcttcggct atttgctacg tctactagta gccttgggca agaagaactc
    18001 ggttgagctc gagatccagg agatggagtc aagagctaag gaggaggcac aagctgttat
    18061 agaaagtgca aacaaggagg ctaaggctgt gctcgatgac gctgaggtca acattcgtga
    18121 gcgtgaggat aggttgctca agaatgaaga ccgtttgata aagaaggagg aactattaga
    18181 caaaagacaa tcagacttag acaaagaggc tgattatatt aaggagaagg ttcaagagat
    18241 caagcagatt aaggagaggg ccgacgagtt ggttgtagct cggtccaaag aactctccag
    18301 agtcgcttcc ctatctgagg aagaggccaa gaatgagctt ttcaaaagaa ttgagcagag
    18361 tgctgaggag gatctattgg taagaaccca aaagctagag aatcaaggtg aggaaagact
    18421 gggagaaagg gctaaggaga tactcgccac atcaatacaa cgtttggcga actcaatatc
    18481 aaacgaggtc atgtcgacca atgtccctct cgcatctgac gaggttaagg gcaagatcat
    18541 tggtaaggag ggtaggaaca tcaaagcatt tgagcgagtt acgggcgttg aggtcattgt
    18601 cgatgacact ccgggggcaa tcactatctc atctttcgat ccagtgagac gtgctgttgc
    18661 caagcttgct ctagagaaat tgattgcaga cggtaggatc cagcctgcaa agatcgagga
    18721 attcgtagag aatgccaaga acgatataaa taaagttatt aaagagaagg gcgagcaagc
    18781 tgcattcgag tgtggggtat ataacctcga tccaaagtta ctctctatcc ttggtcggtt
    18841 acacttccgc acttcttatg ggcaaaatgt tttggagcat tctgtcgaga tggcgcactt
    18901 gtctggaatg attgcagagg agcttggagc aaatgttttg gttgcgaagg ctggtgcact
    18961 tcttcatgac ttaggaaaag cacttgatca tgaggttcag ggtacgcatg ttgagatcgg
    19021 taggaggata ttgcagaagt ttaatgtcga tgaagcagtg atcaaggcta tgcaggcaca
    19081 tcacggtgag tatccatatg agactgtgga gtcgatgatt gtccaaactg ctgatgcaat
    19141 ctctggtggt agacctggtg cacgacgaga caccgtagaa cattacctaa aacgcctaga
    19201 agagctcgaa aacattgcaa catcctttga tggagtggag aaggcgtatg ccctccaggc
    19261 aggtagagag atcagagtct tcgtggagcc tggcaaggta tctgacctag aagctcgaaa
    19321 tatggctaga aatatagctt tgcgtataga gaaggagctc aagtaccccg gcgagatcaa
    19381 ggttagtgtc attagagaga acagggttat cgagttcgca agataaggat taatgaagag
    19441 gcgattttgc ggtattgcgt aaaaaatggc ttcatatata atcggtatgg gtttattagg
    19501 ataattttta gtcaatgaat aaggtagcaa ttgtagacgc agtacatgca aagctcggtg
    19561 gcaccaaagt tcaggccgag gaagtagtcg ataccgttat cgattctatt gtcagcactt
    19621 tgaagaaagg tgacgaagtc tctattgcgg gcttgggcat tttctcaacc aagactcgtg
    19681 cagcaaggca agctagaaat cctcgcacag gcgagtctat ctctgtccca tcaatgcgag
    19741 taccgaagtt tcgtcctgca aaggcactca aagacgcagt tcgttaataa caaactgcgt
    19801 gccaaacaaa aatcccgtct tgaagcggga tttttgttac tcgactttgt atttatcctt
    19861 tatttcttcc acctccctat cgtcttctga ttcatttttc ttccttgctt ccttgccaag
    19921 cttaattaat tcctctccat ttaatttgta caactcctta acacgctctt ctaagtgaga
    19981 tctttcatta agtttcggat cctcaagcac ttctgagagt attatttcca agatgctacc
    20041 aatatgtggt cctgcattcg aattagtgag attcatgatg tctccaccgt ttacactgag
    20101 ctgtttaacc gatatcgggt cacggcacac caactcaatc atcgagtgat acttacgcag
    20161 tcggtatgtt actgccttag gtctacccat accggttcta tcggatgatc gtacatccat
    20221 caggtcccat atgagatctg gacccacatt cttaacgatt ctccttactg ctgatagtga
    20281 gattgtctct gtctcagaga agaacatgtg ccaccttact agtttgacca ccttctccat
    20341 tatttctttg gggaacttca agtctgtaag aatgtttcta gcaatttttg ctccaaccac
    20401 ctcgtgtccg tagaaggtcc actggccgtt aatcatcttc ctggtgtgag gcttggcaat
    20461 gtcgtgtaga agcactgcga ttcttacatg tagtgggaat tttttctccg cagcatagtc
    20521 gagcgatcta atgaggtgct cccaaacagt gtagatgtgc gccttgttct gctctacccc
    20581 cacccccttc tctagctctg gagaaatata tttaagtaga ccgagttgtg tagccaagcc
    20641 aagagcgaca gacgggttgt ctgacatcac aatttttata aactcgtctc ttatacgctc
    20701 tttggatatt tgagaaagga gatccttctt gacctctatt gccgttacag tctctgactc
    20761 gatggcgaag tttagctcgg cagcgaacct aatagctcgt agtattctta atgcatcctc
    20821 cccgaatctt tcattcgggt cccctactgt tctaattaat ttattcttta ggtcttctct
    20881 accatcaaat agatcaatca cttccctatt ctctatatcg tatgcgagcg cgttgatggt
    20941 aaagtcgcgc ctcttcaagt cttcgtctag ggttttagac catttaacct cgtctggtct
    21001 cctgaagtca gaatatgttc cctcagtacg aaacggagta acttcgatca ccctcaaact
    21061 ctcagtggaa ggatcatcat ttactatgcc cacagtgcca tagtcatttt catagaaggt
    21121 gtgttcaaag agtggggtga tttcctctgg tgttgccaca gtagtaagat cccagtcttt
    21181 tggctcttta cctagcaaaa tgtccctcac acagcctcca actagataat tctcaaattt
    21241 ggtactactc aaaacacgcg agactcggct cacttcctct ggaatctcta gtttttcttt
    21301 aatcttggcc atctcatttg tttgcactta gtgtgttgaa gtgtgccccc agcaggaatc
    21361 gaacccacat cccttgttcc gaagacaagt actctatccg ttgagctatg ggggcnnnnn
    21421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtgcg gccagggaga atcgaactcc
    21541 catctcatcc ttggcaagga cgtgttctac cactaaacca tggccgcaag tatgaagata
    21601 ttagcctaaa agtgctataa atgctagagt aaatctctcc acggagtgta atacagcggt
    21661 agtatacacg attcgggttc gtgagacccg ggttcgattc ccggcactcc gaaagtgtat
    21721 aatctaaccg agcacggact gtagtataac ggtagtatac atccatgggg tggatgcggc
    21781 ccgggttcaa ttcccggcag tccgacaagg tttcgttggt ggttcagtcg gggcgtggtg
    21841 taacggctaa catgcttgtt ttgggtacaa gtgactccgg gttcgaatcc cggcgccccg
    21901 actgaggctc taactagaca caataggcct ctaaataagg atattttaaa taagcgggat
    21961 tagtttaatg gtagaataag agttttccaa actttaggtg ggagttcgat tctcccatcc
    22021 cgcacactgt ggtttctgtc tcttaagtgt cctttttgtc tgtatttagt ctattctcac
    22081 gaatttgggc ttcaagctca tggagtttgc cttcccatat cttctgatat tttgcgagcc
    22141 aggtggatac tctcattaga ctgtccggtc taatgttaca catgacacgc ctcccccgct
    22201 tttgggtggt gatgagctca gactctttaa gtattcttat gtgcttgagt actgctggta
    22261 aagatatttt gaatggtgca gctagctccc caacactctg ctcacctctt actaattcat
    22321 ccaaaatgtg cctcctagta gaatcagata tggcatataa catgcgatct agctctacct
    22381 tttgatactt aaccatatgg ttaagtatac ataaaatatc ttgttaaata tagatatact
    22441 gtgaacagta tcaaaataca ttctgtatga tttgcacttt cgcctctttt tcaaagatat
    22501 tgatgcttac gagaaccagg tctagctgcc agtccatatt agtcctcttt tccgtcagat
    22561 aagtctgtat tgtcctctct aaccttttga tcttctgagg gtgaatgttc tcctcacctc
    22621 taattcccgt ttcagatgaa acagatttaa cctccacaaa atgaatgcgg ccagacttac
    22681 tgcatactat atctatctca ccccattttc ttgagtagtt cctttcaatg atctcaaatc
    22741 catgtttctc aagaaacaca caggccacat tctcccctat atcaccaaaa cttcttttag
    22801 ccgtcttagt catgtttctg gtgtaacatc tatatcatat aataaatagt ttcacgtgaa
    22861 atgtatatat tggttaatct agaataatgg cttaaattaa agacttttgt cttacttgtc
    22921 ctaaataata tgtcttttat taacactttc cacacagtta tccacatata tcaaattaat
    22981 ctctaaatac tggcttcatt aagccataat gaaaacccgc ccgaggtttt ctcgagcggg
    23041 ggatagtcgc ctaagcgaca ggaaaggtag ccccagttaa agacacatga ggcagagacg
    23101 acagacttca aaatataaag attggggaag tgtcgtatct gcgattgagg tctttgacca
    23161 gaacatcttt catgggtgcg cggcccataa gaacaatgct acagacttcg ttgctgaatg
    23221 ggagaaaaca acatctccgt ctggcgtctg aaataagtat atgcttaaag gacaaatagt
    23281 taaataggct ggctgtggat aactactatt tgatgtcctt aagagtgatc tgtataggac
    23341 agtgatctga gcccaaaata tggctaagaa tattaacgga ttctactttg tctttgagtt
    23401 tattcgatac gaagaaataa tctattctcc agcccacatt tctttctctt gatttactga
    23461 agtagtgcca ccatgtgtag tgaccattgc ctttagtaaa cattctgaat gcatcggcga
    23521 acccggcgtt tatcatcttg tccacacctt cgcgctcttc gatagtgaat ccatgctcgc
    23581 ccacattatc cttaggttta gccaggtcgt cctctgtgtg ggcgacattt aagtctcctg
    23641 tgaagattac tggcttcatc ttctcaagac tgagcacata ctctagaaag gcggggtccc
    23701 acttcttgtg ccttagcttt aaccgggtaa gatcgggctt ggaatttgga gtatagctgt
    23761 taattaggta aaaatcctta tattcagccg ttattacacg tccttcctca ttcgggttgc
    23821 cgtattcgtc ctcaatcagc ttgtacttct cgacaatctg agctgggaag tcagttatga
    23881 cagaaatagg ttcattcttc gaaaatatgg ctgtaccact gtatcctttc cttgttctag
    23941 atgagttcca atgctccttg tattctggca gatctattac tgcctggcct tgttctgcct
    24001 tagtttcctg caaggctaca atatctggtt tgtactcctc aattaggggt aggaatagac
    24061 cctttttatg cacagcacgg ataccgttca cattccatga aattatttgc atttgaatac
    24121 atgatatact ttttttatga agtttattgt acctgtagta gttttgttcc taatattgct
    24181 cggaggatgg ctatttctta ggaagcctga ttcaaccaca aaggagataa ggcaagaggt
    24241 aaccactgta agtaatgggg ttcaagtcac caaaacagac atttctaaat ctggttccat
    24301 gctcccagag ggcttcccta aagatattcc tgtagagatg gctaatataa aggatagtta
    24361 cagagcttat tatgagaagg ttaaagcaac tcaatatacg gttagttata tatccaagca
    24421 gagcagagac actctttgga agacctattc tgactttatg aatggcaagc aatatacggt
    24481 agatcagtca tcatcaagaa gtttgggaca aatctctggg gttaagggtg atgactctat
    24541 gtctgtagtt ataacatcaa gtgggggagt cactcttgtg caaatcgtat atctagacag
    24601 ataacatgcc gagaaacagt aatttaggcc cacttcttat cattctagtt tttttagtgg
    24661 ctttagttgg aggttacgct ataagggagg atcgatggcc aggtttgcct tggagtctcg
    24721 ggcttgctcc aaccaagttt actcttgatg ccgaggcgcc aaagggtcag ttgataagtg
    24781 gtttccccga agatttgata aatatgacca aagacgctcg tattgagcag tctgccaagt
    24841 acttcttggc tgagggagtg ggtaagtcgc tcgtgctaac taccacctac aagaccaaag
    24901 cttctatatc cgagctcttt gctgcatata tagagaagct gactgccgag ggctacacct
    24961 tgaccaaaac aagttcgggc actggcgagt cggagattga ggccttcaac tcagttcaat
    25021 cgatatcgat aaacatttct gtttcgagca atttggagaa ggaagttcga gttgatgtga
    25081 gaaaggccgt ggtacaataa tttgtagatg ttaaaagatt attttaataa agttacacaa
    25141 atgaaaagac tttttttggt ggtaggtttg atcgcgcttt ctttgggtgt cggccaagca
    25201 tatgcttggg tcgagccaac tctcgcacca ccaaacggca atgtcaccgc acctcttcga
    25261 cttgatgcta atggtagatt aggtatcggc atcacaagtc caggaaaatt atttgaagta
    25321 atgagcgaaa tagtgacaag agcatcttct gcttctggtg gtgtagcttc ttctttctcc
    25381 tctgactcaa ctggagctca aatttgggtt aattcggctt atgaccccgc gagcccatct
    25441 acgggttggg gtaggcgagt tacaatagct ccaaacggaa atgttggtat aaacaatcag
    25501 tttccatctg ctaggcttca tgtgaacctc gacgccaaca gagagattcg tgtaggtact
    25561 ggtatttcga ctgctttgac cgacgcatca gtgttcggac cagtactgag cttttcaaga
    25621 ggttcggacg gtgcagatga tatcgccgga atctttcagt acacaacatc aggggctgca
    25681 aacagactcg ctattgcttc gagagacgac atagtttttg ctgctggcgg gggctcaact
    25741 tacccgtctg ctccagaaag aatgagaatt aattctgcag gaaatgttgg tattggtacg
    25801 agtgctccaa acgagccttt agaagtggcc aattcaacta acggaggtag attgatagtc
    25861 tcagatggtg ctggtgcatc tagaagaact ttacttttgg tttcgcctac tgctactaac
    25921 aactttggac gtgttgagtc atacaactac ggtgcctcta ctggtgtgcc tctatatata
    25981 aatagtagtg gaggaggaaa tgtctctgtt ggtaaccttg ctccaactta taaactaact
    26041 gtcgcaggag atgtaaatgt gccaactggt agttgttaca gagtgaatgg tgtttgtatt
    26101 acttctggcg gtgtgtctgg tagcggtaca gtaaacactc tttcgatgtt tactgctgca
    26161 agtaccttgg gtgattcgag actttctcaa caagcagatg gttcggtgag aacgacaagc
    26221 cctactggtt gggtcgatat tggaccaaag aatgctggtt ttaaccacat ttacactgac
    26281 agacctgcta ctattttcaa cacaggagtc tggactattt ccggaatctt tagtgcatac
    26341 aacctagaca accttaggct tcaaaccaac ggtatagaca ggatcacggc cttggcatcg
    26401 aacggcaacg tgggtatcgg tacagcagcg ccaacacagc gccttactgt gaatggtaat
    26461 ttgaacaaac tcggttcttg gataatagga gatgctaact ggtcaccaca aggactcgag
    26521 gttcataata gttcatggga tggagtgtcg aacaataatt acggagctgt cgcgggcggc
    26581 aagctgtttg cttacaacgg tctacaatct ggtggagcta ctggtgccga ggcaacagat
    26641 gggcagcttt atgtagctgg tacttctatg ctcatgggta atgtgggtat aggcaagaac
    26701 aatccatcaa acactcttga tgtggctggt ggcatgaatg ttgaattaaa tgctgatgct
    26761 atctatgcgg gttatgttag gaacactcat ataaacggta atggtctaag tggagaagga
    26821 ggaatatacg gtgttagagg attggcaaat aatggttctg ggtctggtgt atacgggcaa
    26881 ggtgcggcag gagctgcagc cagaggagtt gtcggggcag caaacgatgg ctacggagta
    26941 tacggaattt cgacttttgg gtacggcatt tactgtgctg cagctggtac tggattctgt
    27001 ggtggtacta gagcttggac aaacacatcc gatattaggt taaagaaaaa tgtttcaacc
    27061 ataacatctg gtctagacaa ggttttgaga cttcgtggag tgaactacac atggaagaac
    27121 gatgagacaa acaagaaaaa cattggtttc attgcgcagg aggtgatgga ggttctgcca
    27181 gaggttgtga acaaaaataa tgaaggctat tacacggtaa acgagagttc agtaaatgca
    27241 gtgcttgttg aggcggtaaa ggaacttaag gcagagaatg atgatttgaa agcgagatta
    27301 gagagactcg aggctaagct taaataactt aaatgaagag gctactcatc acagctagct
    27361 tcttcctctt agccacaggc ttcttttcta cacccgcatc agcacagaca gtaggcctct
    27421 cagggttcgc ttggtctgat ttaccacaat caacagctgg gggtgtagac aggggggcgg
    27481 gctggatcag tttctctggt ccgggttacg gagtgatcgc aaaccaatct acaggaaatc
    27541 tctcaggtta tgcatggtct gaacactacg gttggctgtc attcaactct gcagaagtgg
    27601 ctgggtgtcc ctctggcacg tgccagccaa acattaaccc ctctactggt gtcgctactg
    27661 gttgggctag atttatcaat gctcctgcgg gcacgtcagg gccatggaac ggttgggttc
    27721 gtctatcggg cagtaattat ggtgtgacat atagtagtgg gacacagact ttctctggtt
    27781 atgcatgggg agatagcgat attggttgga tgaggtgggg aggacctgga tacactgtaa
    27841 ccatgagccc tcttactcct ccagaggcaa ctataaatat tgtgacagtg ccagctaatg
    27901 ctacctggac gattaaccca ggatcaattg ggggcacagg taacggcact gctacagtga
    27961 caccctctca agcgggcact tcttacacgg tgacgggtgg agctgcgcct agtgggtacg
    28021 atttccctcc tactattact aattcacaag gtgggggttc ttcagccctt attcttccag
    28081 gaacatctat tacctttact attacttata ctcaagcctt caattacgcc ttgagtaata
    28141 gtgggaatgt ggatgtccag aaggcgggta ttgctcagtc tggccagaat gtcattactg
    28201 caacacattc cgctggtaca ggtcaggctg ttactctctc ggcatctgga ctaccaagcg
    28261 gcgcatctat aggcttctct ggccaaggtt gtgctccaaa tgctggaact ccatgtaact
    28321 acacagccac tatttcggtt ctaccgtcta ctgtctctgg tacatacccc attactatta
    28381 caggttctcc actcggcaga acaaccagct ttaacttggt tattacagat tcaccaaaca
    28441 tgataattag ttgttcagct aacccaagta ctgctcaagt gggacaagat gtgacctgga
    28501 ctgccacaat tgttaacgat ccaaacaata actcacccta cacttatcag tgggcaggag
    28561 atggcgtacc atctgatcaa actatagaaa ttactgccac acaatcattt atcacctctt
    28621 actcaacaac aggtatcaaa cagatacaag caacagtgtg ggatgatgtt cgtaaccaag
    28681 cactttgccc aacgagttat atcaatgtgg gtgtgaatcc tgggttcgga gagttctaat
    28741 gtgctaaaat atttaggaca tgcacattga tgagacagaa cttaagcgtc tggtacttga
    28801 ttctggtcta gtatcaaaga aggatattga agatgctgcc aaattctctg aagagaacgg
    28861 tcagagtctt ggtaaggtcc tagtaagtag aggggtgatt accgaagatg agtttagacg
    28921 tctccaaagt tatatcttgg gtatcccatt cgtcgatctg aaaaatcaga agttaaatat
    28981 cgaagtactc tctctcatcc cagagcctat tgctcgtaac cacaacattg tcgctttcaa
    29041 gaagacgtct acgaatctag aggtggccat gcttgatatt gaggacatga ctgcaatcga
    29101 tttcgtcagg aagaaggtgg ggttaaagat cgatgccaga ctcactgaca acgagtcaat
    29161 gaagtgggct ttagtgtcgt accaaaagag cttaaaagac gagtttggat cagtaataga
    29221 gaaggtggct ggttccatta aggccggcag tgccaagggt gacgaaaatg catcagatct
    29281 aaagaagctt gctgaagatg tacctgtggt tagagtggtg gacacactgc ttaagcacgc
    29341 catcgtccaa gatgcatcag atattcatat agaaccagaa gacgagcaag tgctcgtgcg
    29401 ttaccgtata gacggtattc tccatgactc aatggttttg cccaagaaca ctgctgctgg
    29461 tattactgca cgcattaaag ttctttctaa tctaaagttg gatgagaaac gtgtgcctca
    29521 agacggtcgc ttcaagatag atctagatgg agagaaggtt tctttccgcg tctcgactct
    29581 tcctacttac tacggagaga agactgtgat gagaattttg cgagaaaacg catcaggatt
    29641 cacattggag tcccttggtt ttcacggtta tggtcttgag acgatacaca acgctatcaa
    29701 gctaacaaca ggtatgatcc ttactaccgg tccaacagga agcggtaaga ctaccacgct
    29761 ctacaccgtg ctcgacctag tgaacacccc agatgtaaac atttcgacca ttgaggatcc
    29821 tattgaatac cagatgaaga gagtcaatca aactcaagtg cgtccagaga tcggcttcac
    29881 tttcgcgcag ggtatgcgta cgcttgtgcg tcaggatcca gacattatca tggtcggaga
    29941 gatccgcgac aacgagactg ctgcccttgc tgttaatgct gctcttacag gacacttagt
    30001 gctctccact cttcacacca actcagcagc tggggccata ccaagacttc ttgatatgca
    30061 ggtcgagccc tttctactaa cctctactct tcaagtcatt gtcgctcaga ggttggtacg
    30121 aagacttggc caaggtaaga agaaatataa tttaaacaag accgagatag agtccctcgg
    30181 acacctcata gacttgaacc gactcctaac aatgcttaag gctgagaagg tggtgccaaa
    30241 ggatgctgat tggaaagatg tgccattcta caagccgaac ccggaggatg ataatgaggg
    30301 ttatcagagt cgtattggta tccacgagat acttaaggtt accgagccta tcaaagccct
    30361 tattttaaag aacgcacccg gcaaggagat tgaggaagtg ggcaagaaag acggtatgat
    30421 gactatgcta gaagacggca tttacaaagc ggtattggga caaacatcaa tagaagaagt
    30481 tctaagagta atatccgaat aacatgcttt acacctacac agctaagaat gccaagggag
    30541 aagtttacac aggtgatttc gaggccaaga gtaaaataga ggtatatacc aaggtgagag
    30601 acgagggagg tagggtactt ggtgtaaggg agaagggtta tgtaaatctc aacctttccc
    30661 tagactccat gtttagcagg ataaattcta gagataagat tgttttcgct aaaaacttga
    30721 gctctatgtt atcagcaggg ctttctgtta cgagagcact cgaagtgatg gagaaacagg
    30781 ccaggaagaa tgctttaaag aaacttttgc acgatttggc tcaggatgtg agtaagggta
    30841 ttcctttttc agaggctttg aggaagaaac caaaggtttt tcctccaatt ttcgtatcta
    30901 tggcccttgc tggtgaggag tctggcaacc tatcagattc tttaggtgtt atagcctcgc
    30961 aaatggagaa gtcatacact ctgactaaaa gagttaaggg cgctatgatc tacccagtag
    31021 ttatcctctc gcttatggtg gtgatctcaa tactacttct tatctatatg gtacctactc
    31081 tcacctcaac ttttagtggt ttgggagtag agttgccaat aactaccaga gccattattg
    31141 ctgcatcgga tttcatgatc agacatactt tgattttcat tgcgactatt attttaagca
    31201 ttgttttggt ctttgcattt gcgagaagta aggctggagg aagacttctt gagtcacttt
    31261 tcttgaagct acctgtggtg gggttaatta ttatggaggt taatagcgct cgtactgcac
    31321 gcacaatgtc ctcactactc tcggcaggtg taccaatagt gaatgctctt gaggttactg
    31381 agtctgtggt accgaaccat ttgtacaaaa ttgcgttaat tgacgctaga aactcgatac
    31441 aaaaaggcga gacaatttct actgtgctcg cccgatatga gaagatttac ccaccattca
    31501 ttacggagat gacggctgta ggggaggaga cgggtaagat ctcagaaatg cttctcaatg
    31561 ttgcactttt ctatgaagag gatgtagatg ataagacnnn nnnnnnnnnn nnnnnnnnnn
    31621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31681 nnnnnnnnnn nnnnnnngtg gggggtactc cagccggagt gcttttagcg agtcaaaatt
    31741 tggtccgaga tggtatcaac tggactgtta ctacaacagt ggtaaatgtg gatgacccat
    31801 tcgacgggat agccccaaca gatactaagc ccgccgacta taaactagtg gaggtgatgg
    31861 tggggtgtga tacgtgcaaa aatttcagtc ccgtagctgt cactggaatt gttgcaccaa
    31921 gaaacttaga gccataatgc ccaaacaagg atttacatta gtagagtttg tcatggcggt
    31981 agcgattatg atttctatcg tcttcggtgt cactacttta gctcgggatg tcttggtgat
    32041 aaatacgagt gcaacccaga acctcgtagc acagtctgat gctagaaaag ctcttaagag
    32101 cattattgcc gagcttcgaa cagcagcgcc ttcctctact ggtgcttata caatagagtc
    32161 tgcttcatca accagtgtca tcttctttgc tgatctggat aacgataacc gaactgagag
    32221 gataaggtac tatctggatt atccaacaag gactttaagg aggggtgtag tcgtaccaac
    32281 aggcaacccg ccttcatatg tcttaggaca agaaacgtat acaactctct cgtccgacat
    32341 cgtcatctct acaaccactc ctgtttttga atatttcaac acgaattatg caggcacatc
    32401 ttctcctcta acatatccta ttgatgtgtc tgttattaga ttggtgagag taagactgat
    32461 tatagacaag gatccaaata gggcaccttt agaacaaatt actacttctg aagtgacact
    32521 aaggaatctc aaggataact tatgaaaaaa gaaaaaggat ttttaataat tatagtactt
    32581 gtctttgcct ctatctctat tgctgcgatt atcggtttaa ttagctttac caacattaac
    32641 ataatgcttg cgaggagggc ggttgtgagt gaacaggcct tccagttggc agaggccggc
    32701 cttgagtatt atcgatggca tttagcccac gaccaaaatg actttaccga tggaggggga
    32761 gccttcccac atttgcacaa cttttatgat aggaacggaa acttgcttgg tcagtatgag
    32821 ctcacgataa ctccgcccat tactggttct actttggttg gtatgagatc tactgggata
    32881 cccttaactg atccgacagt aagaagaacg gtaacatcaa gtttaggtaa gccatcgttt
    32941 gctagatatg cactagtctc taactcggat atgcgattcg gacaaggtac tttagttata
    33001 ggaccaactc attccaacgg gggtatacgc tttgatggag tagcacagaa tctcgttact
    33061 agtggtgtag ccaactataa cgatccagac cacactggta acaatgaatt tggggtacat
    33121 actcatgtta atccgccacc tggtagtggg gtgaatggta cctttagagc gaacgaagcg
    33181 cctccaacta acccggttcc aaataggcca gatgtttttc tcgctggaag gaggtttccg
    33241 gtaccaacag ttaactttgc aggcattacc gcagacttca ccaatcttaa gagtttggca
    33301 caatctaatg gaaggtactt tgcgagctca acggcacaag gataccaagt aacgtttaac
    33361 accaacgaca cttacactgt ccatcgtgtt acaagcctaa gaccagctcc aaacaattgt
    33421 acaaatacag cgggtcagac tgggtggggt acttggactg ctagcaccac cgtattggtc
    33481 ggtacttatg cgaacccaaa taatggtgtt atatatatgg aagaccatgt ttgggtggag
    33541 ggacaaattg atacagccag agtgaccttg gtggcagcgg caacaagtac aggagttcag
    33601 cggtcaatta cggttaataa caatctcctc tatacaaact acgatggtca agacgtcatc
    33661 tctctgattg cagagaacaa tataaacacc ggtctattta gccttgataa cctaaggatc
    33721 gacgccgcac ttattgccaa tagtggcagg attggtaggt attattatga ggcggattgt
    33781 gcccccaaca acgttagatc taccataacc ttgtatggca tgattggaag ttacttaaga
    33841 tacggttatg cgtatgtcga caatactggt tatatcaata gaaatcttat ttatgatgcc
    33901 agtcttctct atgcaccacc gccatccttc ccgttaacaa caaatacata tcaaatcgtt
    33961 tcttggcaag agacaatcaa cgactagtca tctgttataa tggttgttca tgaaatacat
    34021 tcttgctgcc aactggaaga gtcatccaag taatgcagat gaggccaaaa aattgcttgt
    34081 tggcttggtc aaaaatggcg ctaattttaa gaagttgcac accataatag ctcctccaaa
    34141 cacttttcta gaattagtaa actcaaagat tcgatcgata ggcgtcctag catctcaaga
    34201 catgtcgctt ttacctaagg gtacctatac cggcgagacg accgcagaga tgcttaaggg
    34261 tttaggagtc agagtctcaa tcgttggtca ctcagagaga agaaagcttg gtgagaccaa
    34321 tactctagtt gcagcgaagg ttagccaagc gattaaagaa ggcattgttc caatagtttg
    34381 tgttggagag gatgtgcacg acactgaggg tggttactac gaagtgctta attctcaaat
    34441 aagaaattca ctcgaagggt taaagaagac tgatgttaag aaacttgtag tggcctatga
    34501 acctctttgg gctataggta agtctgcaaa ggatgccatg caaccaggag atctcggcca
    34561 gatggttgtg tatattagga aggtgctaac tgagatgtat ggcaggaccg ccgctgacca
    34621 ggtcactatt atttatggag gttcagtaga acctggtaat gctaaggagt tactaagctc
    34681 tggagcacaa ggtttcttag ttggacatgc gtcactaaat tccaagagct ttgccgagat
    34741 cgctaaggct attctaaaca aatgaaatca gtcagagaga taggggatat aagtggcaag
    34801 aaggtactag tcagagtaga ttggaatgtt cctcttgtag atggtttggt aagagatgat
    34861 cttcgtatca aaagaagttt ggacactgtt aacttcttag ttgagaaggg ggcgagggta
    34921 acacttatga cccatctcga accagaggaa agaacagtag aggtactcaa acattttcta
    34981 ccaactggag tagagatgtt ggaaaactta agacaaaaca agggagaaaa agacaattca
    35041 gaggagtttg ccaaagagct tgctagcaat caagactatt acgttaacga agccttttcc
    35101 gcttcacaca gagagcatgc ttcaatagtg cttgtaccaa agtttttgcc aagtttcgca
    35161 ggacttggat ttataaacga agtggagaac ttgtccaagg ccttcagccc aaaacatcca
    35221 ttcctcttta ttcttggcgg agccaagttc gaaaccaagt tgcctctagt taaaaagttt
    35281 ttgaatattg ctgacggtat ctttattgca ggacttaacg ccaagccagc gtttgaaatg
    35341 ggttttgata aggagccaaa gattaccctt cctattggag atatttcagc accagactta
    35401 acaagtgaga atctagatat tttgaatgaa aagatagaaa actccgagtt tatagtgtgg
    35461 aacggaccgc tcggtaagta cgaggatggg ttaaaggagg ggacactaag actcgcccag
    35521 atgttaggaa attcaggtaa ggaggttgta gtcggtggtg cagacactct cgccgctata
    35581 aaggagctag atatttcgga caagtttacc ttcgtatcta gtggaggtgg ggctatgctc
    35641 gacttcttgg cgagcggtac tctacctggt atagaggctc tgagttaaat tctaggacca
    35701 ccctcttcct ccatcttttg tggtacgagg tgtatatgtg tgtgggctac atctttgccc
    35761 acaataccga gtcttacata gtcagcatgg taatcgtctt ttaattttaa agccaaggtc
    35821 ttagctgctc taaatagctc atcatacaga tcattaggca gatcagtgaa ccatctataa
    35881 tgatccttgg gtaccaatag agtatgaccc tctcttactg gattgatgtc taagaaagca
    35941 atgaatttgt cgtcttcaaa aactgttttc gaaggtattt cctttctacc aattttacaa
    36001 aaaatacaat tttccataat tactaatatg aaatgctatc tactaatact ccacttgagt
    36061 cgaaaagaga gattgtctct tcctcttctg cccacatctc ccatggtcgg tggagataca
    36121 catgccaagt tttgccttca aagttctttt ccccaatatg gtttgccagg cacccctcat
    36181 agctgaaatg acttttgaca taagcgatgc acatactaga caaacctttt tcaccatcga
    36241 cacagtcacg gcaaacctct ccatcctcat ccttaccacc gtattctggt gtgtggcagc
    36301 tttgcattga gtctatgtat cttttgcact tgatgtcgag cgaggtgtaa ccaacttctt
    36361 ttgatggtct tacacaactc ttatctagtc ctttggggaa actatatgtc tcctccaagt
    36421 atcctgtgca agagttctct ttgaagctgg ttagtgggta tgaggttaag ttgcccggtc
    36481 ctccagaaat cactattgct ctctcgcctc tgtttaagac aatactttcg agatgactcc
    36541 tgccgtcggg cgagacgatt tttgcaccct gtgggatgac gccaacatcc gaggcgtagt
    36601 gcaagtactg acttccgact gtatagcttc gtgcagactt accattctct agtctataac
    36661 cggtaatatt tactggatta gagccgttat tctctataac gatgtattca ctcattggat
    36721 cgttttcgta cttgccatta cctctctcga gcctgagtgt tccagagctt ctgctgctcg
    36781 aggtgtagtt agttgattta gtatcgtctt gataagtaga gttcctccct ttgagtgtgc
    36841 tgtcccaacc agagacatcg aacctcatac ttttgggtat cagtatggca aatatgacga
    36901 tacttatagc aaagaaggtt aaaagccatt ccacgaaggc acccttctcc aaggtctttc
    36961 tcataaaggg tataatacca cgatgtcttt agacgatatt ctccacaaat tacttcacga
    37021 tagaggtgtt actgacaagg aggactttct cgttcctgat tacagcaagc tccacgaccc
    37081 tctccttatg cccggtatgg agaagtctcg agacaggata cttagggcta ttaaggatgg
    37141 tgagcacatc ttcgtttttt ctgattacga tgcagatggt atccctgggg ctgtggtcat
    37201 gaaagacttt attagtagga taaagtacga caatgtttcc ttttatatac cacacagaca
    37261 tgatgaggga ttcggcttaa acactgatgc agtcgatgaa gcaaataagc gaggcgctaa
    37321 actaattgtt actatagatt gtggtacagc ggactcggta gaggtcgctc atgctaactc
    37381 gcttggtatt gatgtcatca tcactgacca ccatgaggca aatgatttgc cagatgcgta
    37441 tgcgatagta aacccgaaga ttaaggggag ccggtatccc ttcgatggac tctgtggtgc
    37501 aggggtactc tataaactca ttcaggctat ttatatgaag gagagttttg gtctcccgat
    37561 tggagtggag aagtggtctc tcgacatggt cgccattgcg acactcagcg atatggtgcc
    37621 tttggtaggg gagaacagaa tactctctta ctttggactc caggtactcc gcaaatctcc
    37681 ccgtccagga atacaggctc tttgtaaaaa actagggatg gaccagaggt tccttaacga
    37741 agatgatata ggttttatga tcactcctcg tattaatgca gcatcgagga tgggtaagcc
    37801 ggaagacgct tttaacttac ttgcaacaaa cgatccgaga gaagcagagg aactcgctct
    37861 ccacttggag acgataaata acgagaggaa gggtatcgtc gcctcgatag ttaaagatgc
    37921 taaaaaacat ctaagcgaga gagaaagtat tggaaaggta attgtcatcg gtaacccaga
    37981 gtggcgacca gcactccttg gactcgtcgc taactcactt gtagaagagt atgagagacc
    38041 tgcatttgtg tggggtaggg atggagatga caagcttaag ggttcatgta ggtcgtacaa
    38101 tggctacgac cttttcgaac taatgaatca tgccaaagaa tcttttgtag agtacggtgg
    38161 gcatgcagga gcgggcgggt tctccgttac gcttgagagt cttgttactt tggaggagaa
    38221 tttatccaac gcactactca aactcgagat gaccgaaggc aagacccata atagtctaga
    38281 gattgagatg aaagatgtgg gggaaggctt atacagagtg ttaaataagt tggctccatt
    38341 cggtacaggt aacccaaaac cagtatttag aataaataat gccgagatca aatctctgaa
    38401 gcaatttggc aaagccaagg atcacctaga acttactctc acacctaata tcaaagcgat
    38461 ttccttcttt tcaaatcctg agtcatactc actcttacca accactactt tgtgtaacct
    38521 cgatgtccat ctagagaaat cctacttccg tggtcgccct gacctccgtc tccgaattgt
    38581 tgatataatc tcctcatgat tactatattt accgatggcg cagcgagggg gaatcctgga
    38641 ggaggaggat ggggggctat tatagccaag gaatttgagg tacatgagat tgggggtaag
    38701 gacccatata ccacgaacaa caagatggag ttaacagctg ccatagaagc gcttaaaact
    38761 ctagaattcg gagtagaagt agagctttat actgactcag agtatgtgat gaagggcatg
    38821 accgaatggg tgcataactg gcagaagaac ggttggagaa ccgctgctaa gaaaccagtg
    38881 gagaatcagg acctctggaa ggatcttgtg tcggctagcg aaggtaaaaa catttcatgg
    38941 aagtatgtcg ccggccactc tggtaacgaa gcgaacgata ggtgcgacga gatagcgact
    39001 aaatatgcgg accatttatc cgtagaacta tatgcaggga gcaggggcga gtacccaatc
    39061 agtctcaagg tctaatctta atattctctt agctctttgg ggatttgtct ttggtattct
    39121 cctcgcatca ttgttatcga tacctccact tgtttcaatc ttggttatgt tcgtcgcgtc
    39181 agcgatttat gtgctcgata gaaatgtctt gatccttata accttagttg ctgcatctct
    39241 agggatatta agatacgaca ttaaagattt tcatgagtta gtgccagcag ggaatattgg
    39301 gatagtggtt tcagagcccg agaatagaga caaagatact cgttacacgg ttcttaccga
    39361 tactggtgag aaagttttgg ttacagacga gatctatagc aaagttctgt atggagacga
    39421 agttaggcta acaggtactc ctaagatcct taagccaagt ggttacgctg attacttagc
    39481 caaggacgat attttctaca cgcttgagag agctaaggta gagatccttt ctcacgacaa
    39541 tgggaatggt attaagggtg ctcttataaa ggttaaaggg tttcttatag acaagatcaa
    39601 aaatatttta cctgagccag aagcaagcct actctcgggt ctgatcgtct cgggcaagca
    39661 agcattgccc aaagaaaccc tagagagctt caaagatgca ggggtggtac atatggtagt
    39721 gctctcgggt tacaacatta caatcatcgc cgagttccta ctcattgtct tcgctttcct
    39781 tgggtctaga ttctcgggtt tcattgcact tgtgggagta gtgctcttca ccattatgtc
    39841 tggagcacct gctacggtgg tacgaggagc aattatggtt tcattccttc tctttgggag
    39901 actgatgaaa cgacccggtt cgccccaaag aatacttctc ttcactgcgg tattgatgct
    39961 catgcataac cccaagatct tgctatatga cccatccttc cagctttcat tccttgctgt
    40021 gctcggtttg atatatggag caccaataat tcaagataag ctcgccaagt tggcactgcc
    40081 tgctcacgga cgagcatcgg gacattcgcg agcaatgcca acctggctta ccgagctctt
    40141 ggcaactact cttgctactc aggtctttgt cttaccatta cttttataca gtatgggtaa
    40201 cttctcactc gtgttcctgt ttgccaatct tcttatcctg cccattctcc cagtagtcat
    40261 gcttattggt tttgttggca cgctgctctc attcgtactc ggtgtgctgg ccatgcccat
    40321 taatttcatc acccatactt tcctcgcgta catacttaag attgcagatt tattcggcaa
    40381 ccttccattc tcaagtttag aaatagaaaa cttccccttg tggggaagtt tggtgatgta
    40441 cttactgata tttattatcg tctggcattc tcgaaattca tctccacact ctcccagttt
    40501 aggttagaga agaaagcttc gacgtagttt ttcttctccg aaggagtgta gtctaaacaa
    40561 taagagtgtt cccacatatc gagggcgagt agaggcgtcg ctccttgtag ttgtcctata
    40621 tgttgttcat ctacccaaga agctaggaaa cgatcatctg tcttgtcgta ccaaagtatt
    40681 gcccaaccta ttcctcgagt tagtgccaag gctttaaatt ctgttagaaa agtatccatt
    40741 gagccaaaga gttcaaggag tcttttgcca agttcgcttt ctttatttag ttctttagct
    40801 ccaccagaaa gggaagagaa gtaaatttca tggttcctca taccattgta ttcaaagctg
    40861 aatcgacgaa acaattctcc agctatgtaa gaatcctttg cactatcaag cactgacatc
    40921 ctttccataa tgaggttagc gttcttcaca taaccggcat atagtttaag atgctcatca
    40981 atagtctttt tagaaatgcc gactaagtct tcgatctcaa atttcttttc ttcaaatgtt
    41041 ttcatgtatt ccattatagc agttttggtt ttctctaatc tttttgtttg gtcgagggtt
    41101 tattcaagaa gtggaggcga actcaaggtc tactttctcg acgtggggca gggggacgca
    41161 atacttatag agtcaccgac taaggggagg gtgctaatag atggtggtcc agatagaaag
    41221 gttctaagtg agcttggtcg agtgttgccc ttcggcgaca gacggataga tgtggttatt
    41281 gagtctcacc cagatgctga ccatataact ggtttagtag aagtactaaa caggtttaat
    41341 gtagatacat ttgtatctac gggtatgaga tcagtaaatt ctttagacga gacactggat
    41401 ggggtaataa aagaaaaaga aatacacaaa gttctaggga aaagaggaat gagtctcgac
    41461 ctcggtgggg gagctattct cacactactc tatccaagcg ggagtattga gggtcttgag
    41521 acaaatgaat cctccatagt ctctaaatta actttaggcg atatcgattt tatcctaacc
    41581 ggtgactcta ccaagcttgc tgagtacaaa ctactctctt tgaacaaaga acttctagac
    41641 tcagaagttc ttcaagttgg gcaccatggg tctgagacct caagctctct actatatgta
    41701 caagctgtga gtcctctgta ttcagtcatc tcctcgggca aggataatag gtatggtcat
    41761 ccacacaaag tgactctcga cactctaaac aaggttgaga caaatatatt aaataccgca
    41821 gatgtcggca ggatcctgtt taaaacagat ggagaggaaa tcgaggtcga gtattagatg
    41881 atacccgctt tcttcaaaac agaatgagca ccacgcttgt tgatggtttt gatagcacga
    41941 gtagagaggg taagggtgat ggtcttctta agctcaggca cataaatacg cttcttctgg
    42001 aggtttgggt acttcctaac catgcctgta gggttgaatt gggtagcacg agtacggttg
    42061 gagtaacctc cacccatctt tgagcccttc tttaatactg gacaaacttt tgccatagtg
    42121 ggcattactc taacataatt gatataattc gccaacaagt atggaattta tctttcaaat
    42181 agcaatattg atcatgtcga tcgtcatcca cgaggtagcg catggctatg ctgcccgcta
    42241 ctacggcgac ctgacagccg agtatgaggg gagactcacc ctaaacccag ttaagcacat
    42301 tgaccctgtt ggttctgtct tcgtgcctct agtgtcttat ctatttggcg gattcgtctt
    42361 cggttgggcg aaaccagtgc cttataaccc ttataaccta aagcctggtc gctggcccga
    42421 ggcgttcgtc gccttagccg gtccactctc aaatattggt ttggcagtag tatttggttt
    42481 acttcttagg tctataggtg ccccaagtga gagttttgtt actatcacag cagggatcgt
    42541 ctatataaat gtgctcttgg cagtctttaa cctcatgcca gtaccaccac ttgacgggtc
    42601 taagctccta ttcgcactct ttcctgatag gatctataag ctcaggagct tcttcgagcg
    42661 ctatggactc atcttggtcc ttttcttcat cctttttgtt tggcagctta ttgctccttt
    42721 ggtcagcatt ttcttcacat tcttgaccgg tttgtctata taagatagag tattggtctc
    42781 cattaggggt acctgcctag agcaggaggt tttgtaaaat gtctaccata aaccaactag
    42841 taaagaagag ccgcaacaag ccaaagagaa agtctaaggc tattgctttg acgcgcgggt
    42901 tcaacactat caagaaccgc ccagtctttt tcaactctcc atttaagcgt ggtgtatgta
    42961 tcaaggtgac taccaagacc ccaaagaagc ctaactccgc aattagaaag atcgctcgtg
    43021 tccgcctaag taacggtcag gaagttactg cctacatccc tggagaggga cacaacctac
    43081 aagagcactc agtggtcatg ctccgtggtg gtcgcgttaa ggacctaggt atccgctaca
    43141 ctattgttcg tggagtactc gatactcagg gagtggagaa gagaaggaag ggtcgaagtc
    43201 agtacggtaa caagcgccca aaggccaagt aatatatgag aagaaaactt aaagcaaaac
    43261 caaagataac cccagactca gtctatgact cagagaaggt ggctaagttc atcaactatg
    43321 tgatgctcga gggaaagaag accactgctc gtggtgttgt ttaccgagct ctcgaatctg
    43381 tgaaggagct aagcaagaac ccaaatcctt tggagatctt cgagacagcc cttaaaaata
    43441 ctggtcctac tatggaagtt cgttcaagaa gagtgggagg tgctaactac cagatccctc
    43501 gtgaggttag accagagcgt cgtcaatatc tatctatgaa gtggattgtg gatgcagcta
    43561 gaagcaagaa gggtaagcct atgcatgaga agttggccga ggagatcatc gcagcttcta
    43621 acaacgaggg cgaggcaatc aagaagagag agaacaccca caagatggcc gaggccaaca
    43681 aagccttcgc tcacttggct tggtaatttc gctccttgtg tttttgtaag tcgaaatgta
    43741 ccctaaggtt agacacattc tcaccttgag gtaacgatga tactttcggg tttttctccc
    43801 aaccagcagt ctatgtatgg tgtcaggaag caggttcggg tcatcagggt gaccggtact
    43861 gtgaacgatc cgtggtacac gatcaagaac ccgttcggca agaagtacat cctcggcgtc
    43921 agggacctcc atggggtcaa ggtcttgggg gtcttcaaga gagggggcac taagaaacct
    43981 cgggcatgat gcctgaggtt tttcatttga ttatctgtct aacttgcggt atcgtatcgt
    44041 gtatctaagc catttaggct ttttattttt atcccaacaa attaataatt tatggaacga
    44101 gattatcctt tagagagagt tcgaaacttc ggtattattg cgcacnnnnn nnnnnnnnnn
    44161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44221 nnnnnnnnnn nnnnnnnnnn nnnnntatgg aacgagatta tcctttagag agagttcgaa
    44281 acttcggtat tattgcgcac atcgatgcag gtaagaccac gacttcagag cgcgtgctct
    44341 tctacacagg agctcagcac aaaataggtg aggtacatga gggtgagacc actactgact
    44401 ggatggagca ggagcgagag cgtggtatta ctatcactgc tgcggcgatt acttgtttct
    44461 ggggaccaac atatctttct gaaagcgaca ggcagaaaaa ggagaaaaag ttccgtttca
    44521 acgttatcga cactccagga cacatcgact ttacagtgga ggttaagaga tctcttcgtg
    44581 tcctcgacgg agcggtagtc gtcttcgacg gtgttgcggg agtagagccc cagtcagaga
    44641 ctaactggcg ttatgctgag gaggcacaag ttcctagaat ctgcttcatc aacaagcttg
    44701 accgcacagg agcttctttc gagcgatcat ataaatctat tctagacaga ctttcaaaga
    44761 aggcggttag attccagatt ccaataggag aagaggacaa gcacgaaggc gtaatcgatc
    44821 ttctcaagat gaaggcgtat tacttcgagg gcaacatggg taaccaggtt atagagaagg
    44881 atatcccagc tgagtacatg gaagatgcga ctaaatatcg tggtgagttg gttgagagga
    44941 ttgtagagaa tgacgagggt cttatgaatg cttatctaga aggtaaggag atctctatcg
    45001 aagctctcaa ggagacgatg aggaaggcag taatcggtaa caagattttc ccagtgatga
    45061 ccggttctgc tcttaagaac aagggagtac agctagtcct cgatgcagtg gttgactatc
    45121 ttccatctcc actcgatatt ccaccagtta agggtctcaa cccaaacacc aacgaggaag
    45181 tgctacgtca tgcagatgac aaggagcctt tctctgctct tgctttcaag ctccaagcag
    45241 acccattcgt cggtcagctt acattcttcc gtgtgtactc aggaacaatt gagagtggtt
    45301 cctatatata caactcaact acaggcaaca aggaaagact aggacgcatt gtccgcttac
    45361 aagcaaacca aagagaggag gttaagaagg ttttcgcagg agagattgct gctgcggtgg
    45421 gactcaagga ggctaagacc tcacacacat tctctgacga ggccaatcca atcatccttg
    45481 atcagattac cttcccagag ccggtgatct cgcttcgtat tgagccaaag actaaggctg
    45541 atcaagagaa gatgggtctt gcactcaagc gtctttctga cgaagatcca acattcagaa
    45601 tcaagtctga ctctgaaacc ggcgagactg tgatcatggg tatgggtgag ctacacctcg
    45661 aaattatagt cgaccgtatg aagagagagt tctcagttga ggcaaatgtt ggtaagcctc
    45721 aagttgcgta caaggagaca attacaggat cagcagaggt ggagaacaag tacataaagc
    45781 agacaggagg taagggtcag tacggtcacg ttaagatcac tatcaagcca ctcgaagctc
    45841 ttgatccaga agctaagatt cctaagaatg tgcatcgtga ggaaggattt gaattcgttg
    45901 actctattaa gggaggagtt attcctcaag agtttattcc tgctgttgag aagggggtta
    45961 gagaggccat ggatcgtgga atacttgcag gcttcaagat gactgatatt tcttgtgagc
    46021 ttacatacgg ttcttaccac gatgtcgact cttcggaaat tgcttacaag atcgctgcct
    46081 cacaagcatt tcaagaagcc gctaagaaag ctaagcccgt tattcttgag cctatcatga
    46141 aggtagaagt agttgtgcca gagaagttca tgggagacat tactggcaac ctctcaagta
    46201 agagagcaca gatcgaggcg atggaagaca ggaatgaact caaggttgtg cgtgccaagg
    46261 tacctctatc tgagatgttc ggttatgtga ctaaccttcg ctctatgaca gaaggtcgag
    46321 gatcatcaac aatggagttt gatcattacg ctatcgtgcc taagaatgtc gaagcagaca
    46381 ttatcgagaa gagaactgga gctaaggctg cctaataaaa taaattcgaa agacaaaacc
    46441 tccttgacca caaggaggtt ttgctttaac ttacgacgca gactgtagga cgacgagcaa
    46501 tccagccgtc gaacgatagg ggacgagaat agtgcacatg tctcggagtg gcggacaggc
    46561 tcgcgcgagg gctcatggtc acaggggtaa caagcgtcga agcaagaaac cgaagagcac
    46621 gcctgtgagt ctgcttgtct cggagaggca gaagggagtg gtgaagtggt tcagtgacaa
    46681 gaagggtttt ggcttcattg gtctgaggaa ggggactccc gatgtgttcg tccattactc
    46741 gagtatcctc ggcacgggct tcaagactct ccctgaaggt agcaaggtgg ccttcgacat
    46801 cgagaggacg aagaagggtc tctcggccac gaatgtgagg atcctcagct agatacccac
    46861 caattggtgg gtttttcttt tctagatcct catgtatagt gcttcctggg ccctcattct
    46921 ggaggtaggg atgtacgaag actcaagtgt attggcagaa aataccccaa catcagagga
    46981 gtggaaaaga ctcgaggagg tgtttgaaag cggggtcgct aaggacataa tcctggcact
    47041 caaggagtca ggcaaccgtc ccaaggatgt ggctttgctc aactcgagga tgagacaagg
    47101 ttcgcttgga gggatcaact catcacttct ccgtcagggg atcccattcc ggctcgaggt
    47161 cgttcacaac aattgtctct tcagggagaa tcaggtccac ttgaagaggg tgcttctccc
    47221 caagccaaag atcgttgaaa ctccgaggga gaactccaga cgctgctgct agcagcgtct
    47281 ttttctttgc tgggattgac tattattggg ctagacagta atatgtcgcg agcgctatac
    47341 cgcttctgct tgttctttga gaccttctca atagggctta ggttaagcgg ttttgcgttc
    47401 ctcgtcacta cgaggttgaa tcggaactgg aagaagatgt gttctcgaac ttcttctgtt
    47461 tctgaaaaaa acttaattaa ttaaatttat acataaaata tatggcagac gcttttgatc
    47521 gctcaaagcc acacgtaaac gtcggtacta ttggccacgt tgaccacggc aagacgactc
    47581 taaccgcagg tattctccat gtcctcgata tgaacaagga caagggttac ggtgcccgtg
    47641 ttgaagcaat tgataagatc gactcagctc ctgaggagcg tgagagaggt attactattg
    47701 ctctccacca ctctgagtac tggacaccag ctcgtcacta cgcacacatc gatgcgccag
    47761 gacacgctga ctacatcaag aacatgatta ccggtgccgc tcaaatggac ggtgcagtgc
    47821 ttgtggtagc cgcaaccgac ggtgttatgc ctcagactcg cgaacacatc ctccttgcta
    47881 agcaagttgg tgtgcctaag attatcgtct tcctaaacaa gtgtgacatg gttcctgaca
    47941 aggagcttat cgacctcgtg gaagaggaga tccgtgacct tcttaccaag cacgatttcg
    48001 acggtgctaa cgcacctgtg atccgtggct caggtcttaa ggctcttgag gctaaggata
    48061 tgagtgatga gtgggcacaa aaggttttcg agctcatttc tactcttgat agctacttcc
    48121 caaatccagt tcgtgagatg gacaagccat tccttatgcc aattgaggac atcttctcta
    48181 tcgaaggccg tggaacagtg gttacaggtc gtatcgagag aggtatggtc aaggttggag
    48241 aggacgcaga gatcgttggt ctccgcgaca ccatgaagac tactgttact ggaattgaga
    48301 tgttcaacaa gcagctccaa gagggtatgg ccggagacaa cgccggaatc ctactaagag
    48361 gtgtgaagaa ggaggaccta tctcgcggtc aagttatcgc gaagccaggt tcagttactc
    48421 ctcacactga gtttactgcc gaggtttaca tcttgaagaa agaggaaggt ggtcgccaca
    48481 ctccattctt cactggatac aagccacagt tctacatccg cacaactgac gttactggag
    48541 aagttactct aaacgcaggt gttgagatga tcatgcctgg agatacagca accttcaatg
    48601 tgaagttgac tgctcctgtc gcacttgagg atcagaccca cttcgctatt cgcgagggag
    48661 gtaagactgt cggtgccggt gtcgtaacta aggtagttaa gtaatctgtt cgcaagaaca
    48721 aagccacaat ggctaaagca aaagctacaa ccaaaaaagc tgtgaaagcg ggagcaaccg
    48781 aaggtgcggg taagctccgt atccgtgtca gagcttatga gagcaagatc cttgatgtat
    48841 ccgtaaagca aatcatcgac actgcactcc gctatgatgc agagatccag ggtccgatac
    48901 cacttccaac agagattaag aagtacactg tgaaccgcgc tgcgttcgta tacaagaatg
    48961 ctcgagaaca attcgaaatg cgaatacaca agcgcttagt ggacattgtt aatccttctg
    49021 gtaaggtaat cgaggctctc acaaacttgt ctcttcctag tggagtgaac attgatgtga
    49081 agatgatcta gttcgacaag ctcactacga gtaaaaacat gaaatccatc attaccaaga
    49141 aagtaaatat gacccaaatc ttcgacgaaa gcggacgggc tttgcctgtg actgtgcttg
    49201 tcgctgccga gccaattggc gagggggccc ttgtagaggg tgatatggta gtcatcacag
    49261 gtacttctaa aggtaaggga ttccagggtg tggttaagcg tcatggcttc aagggaggca
    49321 gacgctcaca cggtcagaag cattcagagc gtgagccggg ctccattggt ggaggtgcac
    49381 gcgctggagg tagagtagct aagggtatgc gcatggcggg tcgtatgggt aacgaaaccg
    49441 ttactcagaa gaacctaaag gtcgttaaga ttcttccaga atctaacgaa atctttatcc
    49501 atggtgctgt ccctggtaga cgaggaagca aggtaatgat caagaccaac taacatggaa
    49561 gcacagatat acgacaacaa aggcaaggag gcaggaaaga tcaagctacc agaggagctc
    49621 ttcggtctct cttggaacgc agacctcgtg caccaggtag ccacatctct tcagagcaat
    49681 atgaggaaga acattgctca cactaaaaca cgtggtgagg ttcgtggagg aggcaagaag
    49741 ccttggcagc agaagggtac tggtcgtgct cgccatggct ctacaagatc tcctatctgg
    49801 gttggtggag gtgtagctca tggtccaaga aacgacaaga acttccaaag agtggtcaat
    49861 aagtctatgc gtactaaggc tctttacact atcctctcga ggaagttcaa agagggtgaa
    49921 gtgctcttcg ttgatagtct caacctctct acaccaaaga ccaaggatgc tgtaactctt
    49981 atgaagaacc tctctaaggt gaagggcttc gagaagctag agaacaagag aaagaatgca
    50041 gcactcttcg ctacagctac taagagtgtt ggtaccgaga agagtatgag aaacattgga
    50101 agcctagagg tggtggaggc aagaaatttg aacccgatct cacttctaag ttacaagtac
    50161 ttggtgatcg agaatccaga ggcatcactc aaggtcctcc caggagcaaa ataattttat
    50221 ggctgaaaag aaaacaacaa aaaaggagaa gaaggtagtt gagaaggcag aagtgtctaa
    50281 gtctgttatt gtctctccaa gaatgactga gaagtcagta ctctctcagg agtctaaggg
    50341 ggtctatgtc tttaatgtga agcgtgactc aaccaagaag caaattgctg cttctgttaa
    50401 ggcagactac aaggttactc cacttaaggt tagaacctca caagttcctt cgaaacaaaa
    50461 gttctctcgc ggtaagtggg gtgtgaaggg tggtggcaag aaggcgtatg tatatttgaa
    50521 gaagggcgat aagattgctg ttgcataaat accgaaatga aacgatacaa accaacaaca
    50581 aaatcaagaa gaaacatgac tggaatcaac ttccgcaagt tgatcacatc tactactcca
    50641 gttaagtcac ttactacagg atttaagaga ggggacggac gtaacaacac tggtcgtatc
    50701 accactcgtc acaagggagg tggacacaag agacgattcc gcgaaattga cttcgtattt
    50761 gataagaaga atgtgccttt cactgtgaag acaattgagt atgatccaaa ccgttctggc
    50821 ttcatcggac tcgtagcata caaggacgga gagaggagat acatcctact ttccaagact
    50881 atgcgtgttg gagatacttt cgttgcgtct gagacagcaa ccattactcc tggtaacaga
    50941 cttccactca agaacatccc agttggtacc tttgtttaca atgtagagct taagccaaat
    51001 ggtggtgcga agatcgctcg ctcagctggt aactatgtgg aagtatctgc taaggatgct
    51061 ggctatgtgg acttgaagat gccatcaagt gaggttcgca aagtcttgga gactgcttgg
    51121 gcaaccatcg gcgaggtgtc taacgatgag aatagactgg tgaacctagg taaggctggt
    51181 cgctcaagat ggctcggtat cagacctact actcgtggta ctgcgatgaa cccagtagac
    51241 cacccacacg gaggaggaga gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51361 nnnagaactt ggttccatgc ccgttgcgta agcgggcttt tttgtttgca atttttataa
    51421 taaagcgcta tatttctgac atgaaacaac ttgatccaag agcagtatgg ctcttttttc
    51481 tttcgggagg aacgcgaata ttggtatttc ttcttgtggc cttttctttg attattcaag
    51541 cttattttga gtacaaagat gctggcgagg cggtccctct attgttgagg ttgtggtggc
    51601 taatacctat attgtgcatg atcgcactta ttatctatgc aaaattgagt tatagttttt
    51661 acaagtatga actcacagat atgggtttca aaaaggagtc tggaattatc tggaagcagt
    51721 atgtcaccat cccttatgat cggatacaga atgttgatat ttatagagga atctttgctc
    51781 gaattttagg cttgtctgat ctacagattc acacagctgg tgttggtggt gtagcaatgg
    51841 gtgagggtag actaccagga ctctctgttt ctgatgctga gaagatgaga gatgaactct
    51901 tggtgagggc taaacagtca cgaaacggtc aggggctcta aaaataaaaa ctcgctcaag
    51961 ggcgagtttt ttgttagggt gataggagat atgacctcaa ccaggagtct attatgtcta
    52021 aaagacatgg tactgctaga ccggttggtt cttttcgtga gggagatgtt gttagtggta
    52081 aaatgatcat catttttgcg actactcgta atactgatct caccgaaggt cgtggtacaa
    52141 caattgacca ttcataccat ttgagcgcac gcgacgcgga ggttggagct catggtatcg
    52201 atgtgatggg atcagatggt ggaatcagag aagttttggc gcttcaagaa gaccaaagcg
    52261 ggagagttta tctcttcgcc aacaacgctt caccagttac catcaaagag aattctgaga
    52321 tggttgccaa tcttcggaaa agagctttga gcaagttgag ttcgagtgag agggcggcat
    52381 tgggccttga tgattagaga ttgtttattg taactcgctc ttgagcgagt tttttgttag
    52441 ggtggtttgt agaatcaact ctggggggct tgtggccaag aagcataagg agatctcggt
    52501 atcagaactt cgtgcagact tgccgatgat tcttcataga atagtgttgc tcaatgagag
    52561 ttacgtggtt aagaagcatg ggaagaaggt ttgtatcata caagcggttc ccggaaactc
    52621 ttctgttaac catccacatg gaggggcgga gtggcacagg tagtcgttgt caggcaccca
    52681 taagtagggc gccttttttg tttgcaattt ttatgggttc ctgtaaagta ttcgacaaag
    52741 gtcaaagaca gaaggtggcg aaagccttaa ttacgccttc cgagatcact ctcatccgag
    52801 acctttgtgg tcgattttat ttgtcccaat ttaaagagac aaacacaaaa caatggcaat
    52861 tagcaaacag aaaaaggggg agataatcga taagctctca aaggctatca aggatgcaaa
    52921 gtctttggtc tttgtgaact tccacggact caaggtctct gacaccacca acctcaggcg
    52981 cgagctaagg aaggagggtg tggagtacca ggtgtctaag aagactctaa ccaagcgcgt
    53041 actcgatacc gagaagttcg agggcacaat gcctgaactc gagggagagc tagctatggc
    53101 atggagtgag gatctcatcg cgcctgcaag aggtgtacat gctttcgcca agaagttccc
    53161 tgaggctcta aagatccttg gtggagtctt cgaaggtaag tacatgagta aggcagagat
    53221 gatggagatc gctactatac ctccagttga ggtgcttcgt ggtaagtttg taaacattat
    53281 caattcacca atccagcgct tcgcaatagc tctcggagag gtagcaaaga aaaagtaatc
    53341 acatgacaca agaacaaatc atcgaggcag ttgagaagtt gtcagtactc gaactcaacg
    53401 cactcgttaa ggcaattgag gagaagtggg gcgtatcagc atcagccgta gcagcaggtc
    53461 cagcagcggg tgcagcacca gctgaggaag ctaagagtac agccgatgta gaactaactg
    53521 atggaggtgc aaacaagatt gcagtcatca aggttgtgaa ggaggtacta gcactaggtc
    53581 taaaggaagc aaaggatatg gtggacgcag ctccatctat gcttaaggcc ggcatgaaga
    53641 aggaggacgc cgaggctctt aagaagaaga tcgaggannn nnnnnnnnnn nnnnnnnnnn
    53701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53761 nnnnnnnnnn nnnnnnngag tgggggtaaa atacgcccac atggggaaat caatttcaca
    53821 cttatttggg agcgaggcaa aggtcaaggt gatgcgcctt ttcattttca ataaaggggc
    53881 aatctttact gcaaagacta ttagcgagcg agtccaagca agtgccaagg tgataaggaa
    53941 agagctcgcc agtctgatca aagccggtct tatatacaag agagctagag gttatgtgct
    54001 taatgccgag tatgattatc tcaaagcagt agaacgcttc ctcatagagg ccacacctat
    54061 ctcggagaag gagatcatca ggaagttgtc gaaggctggc agtatgcgtc ttatcctcac
    54121 ctctggtgtc ttcaagcaag actctgaggc tagggtagac ctgctcgttg tgggggacaa
    54181 gatcaaggag gggagactgc ttaaggctat aagctctctt gaggcagact taggcaaaga
    54241 acttcgttac gctgccttcg agacacctga cttcaagtac cgtctaggta tatacgacaa
    54301 gctagtccgc gatatcctag actatccaca tagaaagttg gtcgataagg tgggtctcca
    54361 acctggggta taatattgag taagcccgca agggcattac tagataaact gtacagtaag
    54421 gatttatgta tatcagcaac tggggaaacg tgcttacgct ctcattccag aatctctgga
    54481 tgggtatcgt tagtttcgtt ccgaaccttg tcgtcgcgct tatcatcgtc attcttggat
    54541 ggctcgtagg tgcattgctc ggccgggcaa tttggcaagt gttccgctct ttgaaggttg
    54601 acgaagcact tcgtcgtgca ggattcgaag gattcctacg ccgtggtggt gtcgatcttg
    54661 actcgggcgc gtttattggc ggtcttgtaa agtggtttgt agtagtagtc ttcttggtag
    54721 cggccttcga tgttcttggc cttactcagg ttaacctctt cctacaacag gtagtgatcg
    54781 cctacattcc tcgcgttatc gtagccgcat tggtcatcct cgttggaggt gtcatcggag
    54841 acgtagtcga gagagtggtg gttactgcag ctcgtactgc agagattcgc tcagcacact
    54901 tcgctggagc agtgtctaag tgggcggttt gggtcttcgc tatattggta gcgctttctc
    54961 aactcggcat cgcaacggct ttcagccaga cactcttcac cggtatcgtt atcgctatct
    55021 cactagcact tggtctctcc tttggtcttg gtggccagga ggcagcagct cgattcatcg
    55081 agaggctccg aggcgacatg ggtggcaagc acaactagtc ttggtattaa gctagataat
    55141 acctaggtgc aaggaggggc tttcgagccc ctttttgcta tccataaagt gcttgacaag
    55201 tagactgaag tatgcta
//
LOCUS       DAORIT010000002        30277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_233_length_30277_cov_55.030706, whole genome shotgun sequence.
ACCESSION   DAORIT010000002 DAORIT010000000
VERSION     DAORIT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30277
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_233_length_30277_cov_55.030706"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="trpS"
                     /locus_tag="PLD99_00355"
     CDS             <1..753
                     /gene="trpS"
                     /locus_tag="PLD99_00355"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRH25390.1"
                     /translation="LAIGLDPKKVTLYYQSDVPHVTELSWIFSCLTTMPELMRAHAFK
                     DKEAKNADINVGTFNYPLLMAADILIHDADVVPVGKDQKQHIEITREVARDFNNTYGE
                     TFKEPKELVLGAVETVPGTDGQKMSKSYGNTIPLFATDEEIEKAVMSIVTDSSGEYPE
                     NVFKMHQLFRTNDELDQIYTDNKGQYKKLKELLAEDIKNFIRPMRERRAEWAGKMDEV
                     DNIVAEGGKRMRKVVHDRMIEVRNKVGLIHND"
     gene            746..2092
                     /gene="aspS"
                     /locus_tag="PLD99_00360"
     CDS             746..2092
                     /gene="aspS"
                     /locus_tag="PLD99_00360"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HRH25391.1"
                     /translation="MTKRTFIKDLSGKKGEEVSISGWVDVRRDQGKMIFLDIRDMTGK
                     VQSVVLPNHTEALEVAKQLRSEWVVRIVGKVNARPEKNINPEVANGDIELEVTSVEVL
                     AKAEPLPFDMSIDGYNLDLVTELDNRALVLRHPKVQAVFKVQETIIDAFREHLKNNMF
                     FEFQAPAITPAVAEGGSEVFQVDYFDKKAYLTQSPQLYKQIVMSAFERVFSVNKIFRA
                     EPSATTRHLTEVVSLDAEMGFIESWEDIRDMAESTVRHILKRVSEDNAEHLKLLKVEL
                     PTMIEKTPTISLTEAQEKIFKEFGRDVRGEKDLNAQDERELCEIIKKETGSDFVFVYG
                     YPTRKKPFYVYPNPENPEFNEGVDLLCRGLEWMSGGRRINKYDQLLEHVKEWKMDPKK
                     IKMFLEAFKYGVPPEGGFALGAERVTMQLLGLKNVREASMFPRDMDRIDERLSETN"
     gene            2094..2822
                     /locus_tag="PLD99_00365"
     CDS             2094..2822
                     /locus_tag="PLD99_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRH25392.1"
                     /translation="MEGFGYSVKTEAFTGPLDLLLDLIEKRKLFVNDVSLSQVTDDFV
                     KYIETHEEFPISESAEFILVASTLMLIKSRSLLPSLPLTEEETASIEDLEDRLKLYQK
                     VKELTVELKKLYGQKIIFEKEPKKSEIIVFSPDSKTDIDNLSNALMHLLNTLPKKEAL
                     PKTTVKKIITLEQMIEKLSERIAKGMRMKFSEVYSKEEKVNTIVGFLAVLELVKRGVL
                     RVNQEVKHGEIEMETDKIGVPSYI"
     gene            2828..3373
                     /locus_tag="PLD99_00370"
     CDS             2828..3373
                     /locus_tag="PLD99_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRH25393.1"
                     /translation="MTLESKIEAILFYKNEPLTHKELSKILEVDVDTVRNALSNLKKS
                     YENKGIVLIETSDSVALGTHPELSSMFEKMQKEELSKELGRAGLETLSIILYKGPVAR
                     REIDHIRGVNSGFIVRNLLIRGLIERTESGMGERSFSYRPTLALLKHLGVTSVKDLPE
                     YGVALQKMNTFIATEEKDVNE"
     gene            3360..4307
                     /locus_tag="PLD99_00375"
     CDS             3360..4307
                     /locus_tag="PLD99_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25394.1"
                     /translation="MLTNKQEIAFFSFLGVVLVFLLTFTYILGSKSVEGQKVVVKKST
                     PLHIGRVDIEAKAAYVYDTLSGEVLYAKNENERLPLASLTKVMSALVASEEVPSYRTI
                     AISESSTKTDGDAGLVPGEHWTLKDLLDFSLVSSANDGIRAIALAVGALDQNNANDES
                     QVSDFVLKMNDLASQIGMKNTYYLNDTGLDESKEQGGAYGSAKDQAILFEYILKKNPT
                     LLASTKSSILRIRNVEGQIHTARNTSSIVNDIPGIIASKTGFTDLAGGNLIIAFDPEI
                     GRPIVISLLGSSEEGRFRDMMVLVKASLASVENSVISTE"
     gene            4304..5356
                     /locus_tag="PLD99_00380"
     CDS             4304..5356
                     /locus_tag="PLD99_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25395.1"
                     /translation="MIVDVVKIFIPSILAFVLGLVITPFVSKILIKNRMWKKKSVVVS
                     TDGSGAPITQALHGDEERKTPRMGGIVIWASALITILVFWLGAEVDGPVFDKLNFLSR
                     NQTWLPLFTLIGGALIGLIDDYWTVSEIYDQKAGGLSFKKRIAMVTIVALIGAWWFYY
                     KLEMTSIIVPFLGVVDIGWFFIPFFVLVMLGTYSGGVIDGLDGLSGGVFAIIFGTYGV
                     IAFLGNQIDLSTFCFVLMGGLLAFLWYNIPPALFFMSETGIMALTMTLAVVAFLTQQV
                     LILPLIAFPLVVSSASSSIQLLSKRFRGGKKVFLVAPLHHHFQALGWPASTVVMRYWI
                     VGVFVSLLGLVIVLLG"
     gene            5388..6506
                     /gene="ftsW"
                     /locus_tag="PLD99_00385"
     CDS             5388..6506
                     /gene="ftsW"
                     /locus_tag="PLD99_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRH25396.1"
                     /translation="MSFKEWRVDKPFFFAVIILAIAGFVSFYSASLGLLAKSSEQYSS
                     VTFSQTVLGLCLGSVAMLVAIRADYKKWKKYAFYLLLFAIFLNILVLIPQVGFAHNGA
                     RRWIEIGGLTFQPSEILKLAFVIYLAAWASKVKEKMGEYKAGFIPLVVLLVLCGTLLL
                     MQPDTDTFVMLATAGVAIYIVGGGRWRDLMILILVGVLGITLLAAFRPYVRQRIFTYF
                     NPQAEAQGSGYQIQQSLIAIGSGGFFGKGFGQSVQKFAFLPEPMGDSIFAVQAEEFGF
                     VGASSLILLFVFFAVRGLKIASNTPDPFGRLVVVGLVIMITAQAFVNIGAMLSILPLS
                     GITLPFVSQGGTSLFFTLLEAGIILSISKSATLIPKRK"
     gene            6526..7659
                     /gene="murG"
                     /locus_tag="PLD99_00390"
     CDS             6526..7659
                     /gene="murG"
                     /locus_tag="PLD99_00390"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRH25397.1"
                     /translation="MRILFTGGGTGGHFYPIIAVAEAIREVAKEKKLLPPELYYFAPT
                     PYNESILFEQSITYKKVQAGKMRRYFSILNFFDLWRTAWGVLSAIYKVYRLYPDVIFG
                     KGGYASFPVLFAAKILHIPVVIHESDAVPGRANLWAGKFANKIAVSWAEAAKYFPSGK
                     VAHTGNPVRGDIRQRKTEGGKEFMKVEEGIPTVLVLGGSQGAEAINEVVVEALPELIK
                     DYSVIHQTGKRNITSSKTTADVILSKSANKDRYKPVDYLNGLELRMAAGAADIVISRA
                     GSTIFEIAAWGVPSIIVPIKDSNGDHQRANAFSYARAGACEVVEEDNLTANILVAEVR
                     RILSSTELSDKMKAGAKAFDRADASKKVAEEILKIALSHEFEN"
     gene            7661..8881
                     /locus_tag="PLD99_00395"
     CDS             7661..8881
                     /locus_tag="PLD99_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRH25398.1"
                     /translation="MNILNKLFGRNKVVVRMAPSPTGPLHVGTARTTLFNYLYAKKMS
                     GKFIMRIEDTDKERSTKEFEQNILDGLDWLGLKYDEFYRQSERTDVYVSYVRKMIKEG
                     TAYISKETPKEPGDRAEVVRFKNPNKKVIFEDLIRDKVEFDTTELGDFVIAKSPEEPL
                     YHLAVVVDDHEMGVTHVIRGEDHISNTPRQILIQEAIGAKRPIYAHLPLMLDADRQKL
                     SKRKHGEAVSVDFYKKSGILPEALLNFLALIGWNPGTEQEVFTLKELCEVFDIKKVQK
                     GGAVFNEKKLEWFNKEHIKLLPEEEQFKILQKSIEHEDYFSRAPDLDITKINWKTVPV
                     AETKMHLEKVLMIIDDKDQIMKYAEEAGKGNVLWPLRFALSGKEKSPDPFSLIRIIGI
                     DESRRRVENAIKLL"
     gene            8881..10158
                     /locus_tag="PLD99_00400"
     CDS             8881..10158
                     /locus_tag="PLD99_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRH25399.1"
                     /translation="MKKFLFLLVILFCFFPSPTTAQTNSDILDKIEETKRERDKLLEE
                     QKKIQAALNEISKQGQTLQTTVKSLDTTRSKLANDLKLTQTNINASNLTIKKLEGDIG
                     TNESEIETHKSAIKESLQQIAAYDKQSFVMGLLTYDSLDEVWSDTVSIWDLQEKINER
                     INVLEKTQIKLIENKVAKESQKKQLVSLSSQLSGQKRVADETRTAQALLLSATKSKEA
                     EYQKMLAENKAREEEFERLLFQFESALTASDASERPTGGKGSLAWPLDNVFITQQFGR
                     TSASTRLYVSGTHNGIDFRATVGTRVKSVRDGVVSGVGNTDDQRGCYSYGRWILIRHN
                     NGLSSLYTHLSGSIVSAGQEVTEGQVIGYTGGQPRQYGSGYSTGPHLHFGLFVTAGVQ
                     VLPYSSSKNCQNVSIPLANPKDYLDPLGYLPNL"
     gene            10171..10452
                     /locus_tag="PLD99_00405"
     CDS             10171..10452
                     /locus_tag="PLD99_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25400.1"
                     /translation="MKEKGFVMIIVIVLIALVLLKYFLNWDIFDAASTEQGKSTILYI
                     RDVVNLVWSYISAPVIFIWQRIVWPIVDIAWNNFNNFIDWGRNNYNPSN"
     gene            10783..>11156
                     /locus_tag="PLD99_00410"
     CDS             10783..>11156
                     /locus_tag="PLD99_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25401.1"
                     /translation="MTRDKMVDYLPFSGPDHFGQSDVEEAKRVYIATHPRGSENPSIV
                     VVTGKPEDYTDEEIEKLFKFAKQKTAEWITNASGRPPIMIVIGKVSPRSTGSWVRKRV
                     TWTGGNFFSPTLDEAIQRMASA"
     assembly_gap    11157..11256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11257..11674
                     /locus_tag="PLD99_00415"
     CDS             <11257..11674
                     /locus_tag="PLD99_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25402.1"
                     /translation="LTSPPLVGRVGVVGLYFHPHVRHPMPLVTIYVNDQFLEPNVRMA
                     LAVRVHREIESIVKKSLDVDTVVVQQISVSHLSSIFIDITCNHRETREANLNERRDMI
                     AEWLRGIMEEAGMRTGVVHLRIVLAPVSYCELVVQA"
     gene            11745..12311
                     /locus_tag="PLD99_00420"
     CDS             11745..12311
                     /locus_tag="PLD99_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRH25403.1"
                     /translation="MELEVAKIADFYREEKRMPSYSELQRLLGYKSKSAVHYFADKLI
                     KKGFIDKDDTGRLLPKNLGEIMVLGVVEAGFPTSASEELLDTMSLDEWLVEKKEATYM
                     LTVKGESMINAGILPGDTVLVERGVTPKLGDIVIAEVDGEYTMKYYRMKNKKVYLEAA
                     NDKFDDIYPEESLSIPAVVRAVIRKYKK"
     gene            12318..13550
                     /locus_tag="PLD99_00425"
     CDS             12318..13550
                     /locus_tag="PLD99_00425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRH25404.1"
                     /translation="MQRAILHIDGDSFFASCEVALDPKLKGKPVVTGHERGIATAMSK
                     EAKALGISRGMPVFMIKKLYPSAVIVESHYHIYGMFAQRMYDIVRRYTDVVEEYSIDE
                     CFADITHLGKPGTTYKSIAESIRKDLKKELGMTFSIGLGETKVLAKVASKWEKPDGLT
                     IINKDNIQSISSKLQIGSVWGIGPSSTVELNRHNIRTVHDFVSRPQGYIESNFSRPIV
                     ETWHELRGTQVHHVSQSDESTDDQKSVQSTKSFGKASKDKKFLLSELSRNVERASAHA
                     RQLGLISKHIYIFLKTKEFRYKRVDVQLAQATASPTLIMNEVLKVFDSIYDEETLYRA
                     TGVTLANLVPENLIQEDLFGESEKKSIWKNIFEITDQLDRRYGSRTMALASSLGSMKV
                     RGYKPRRLLNIPSMGETL"
     gene            complement(13687..14130)
                     /locus_tag="PLD99_00430"
     CDS             complement(13687..14130)
                     /locus_tag="PLD99_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25405.1"
                     /translation="MIHKVSPISEIHRVASTDPKEIKSEDYMPITDPANVERFIEDYF
                     SDIPELKKIAFCESSFRHFEKNGEPRRGKVNRYDVGVMQINEMYHAKEASALGLDLET
                     IDGNVAFARHLYEREGSRPWNSSSPCWDKSEDLAINTTSKNIAKN"
     gene            complement(14425..15957)
                     /locus_tag="PLD99_00435"
     CDS             complement(14425..15957)
                     /locus_tag="PLD99_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRH25406.1"
                     /translation="MKNTDTTMQKIKSFIKARTKLQISIFVLVVIIGLFLFTGGDKPK
                     TESVVERGVVEQEVVATGKVRSASEVDLGFERSGKVVMVNANVGDVVEEGATLVVLDQ
                     SDLLADIKKANASLNEALINLEKAKRTSSSDYSNAYNKLYIALIDAYTDARDSVLNTA
                     DRFFWSPKSSSPKLEISHVSNGNDIKFYIDDTLARNISVERGALNDIFATWDLSINGA
                     KTSTNGLDGELALTEKNIDRVKNFLADLSYILNTIKGYDQSYESIIAGYKSSMTTART
                     ALNTSLSELLTAKSSYLDAPNEVSFGSGFDEVLQEESRVEATRADVESLQSELSKTVL
                     KSPISGVVTKQDAKVGEIVTSGMQTTSVVADKNLEIVADISEVNIGKIEADNTVKISL
                     DAFPGETILGRVLYVEPGDKLVDGVVNYEVRVVFVGNTPENIKTGLTSSLAISTERKE
                     DVLRIPAFAVERRSGKRFVIVKTKEGEEEREIQVGILGKDGFVEVVEGLSEGEIVLSA
                     QK"
     gene            complement(15923..16618)
                     /locus_tag="PLD99_00440"
     CDS             complement(15923..16618)
                     /locus_tag="PLD99_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRH25407.1"
                     /translation="MIEAKSLVKKFKSGDDFIYAINDVSFKVESGEFVAITGRSGSGK
                     STLMYQLGLLDHPSDGRIIIDGEDITDLSEVARTSLRLNSLGYIFQDYALIPELTAFE
                     NVLIPLMMQGLTSDEAKKKAVKSLERIGLGDRLDHLPSMLSGGQQQRVSIARAIAHEP
                     KIIFADEPTANLDSESSKLVLENFKELNKEGQTILMVTHEPEYAKMTDRVITLSDGKI
                     LSDEKHRHHHAKN"
     gene            complement(16615..17898)
                     /locus_tag="PLD99_00445"
     CDS             complement(16615..17898)
                     /locus_tag="PLD99_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25408.1"
                     /translation="MKTFGERIEHFGRSISLGWFLAYRQVRRSSFATTALIVFVMTLT
                     FLNLVVVRGVLVGLIQGSTNIYKDNYAGDIIITKLPKKNFIEHSQGIISNLESLPGVR
                     AYTYRYAEGGEIEAKYKEKIRQTDDANSAGTTIVGLDPDRENTVTQISKFIIEGRFLN
                     SDDVDGIVLGSSLLKKYLNIESPNISLLEDVEIGDKIRVSIGGFRKEFTVLGFLKSKT
                     DEIDFKAFIVESTMRALVGRGDYNVDEISVVIEPGLDMNLMKESILRTGAGDYARVLT
                     WEESLPKFLLDIQNTFSILGNAIGSIGLVVASITIFIVIFINAITRRKYIGILKGIGV
                     DSSAIEMAYVMQAIFYALLGIAAGLVILYGFLVPFIDAHPIKFPFSDGILVAELGTTM
                     IRALLLLIATIIAGFIPARLVVKQNTLDAILGKTK"
     gene            complement(17936..18265)
                     /locus_tag="PLD99_00450"
     CDS             complement(17936..18265)
                     /locus_tag="PLD99_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HRH25409.1"
                     /translation="MEKSPIKPYFLAIVIVLVALLSFGVGRLTSTQREGVEINFDQSL
                     LQNTASAANVSSSVTTGKISASVNGTRYYYPHCKSTVSEKNKIFFDSAAQAEEAGYEL
                     AVNCKPK"
     gene            18329..>18567
                     /gene="rpmA"
                     /locus_tag="PLD99_00455"
     CDS             18329..>18567
                     /gene="rpmA"
                     /locus_tag="PLD99_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRH25410.1"
                     /translation="MATKKSGGTTKNGRDSNPKYLGIKVNHGTATKVGQILVRQRGTD
                     VLPGKNVGIGKDHTLFALKDGKVTLTYKRKTHFDNT"
     assembly_gap    18568..18667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18668..19085)
                     /gene="rplI"
                     /locus_tag="PLD99_00460"
     CDS             complement(<18668..19085)
                     /gene="rplI"
                     /locus_tag="PLD99_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRH25411.1"
                     /translation="MKVILLKDIKGVGKRYEEKNVSDGYAENFLIPKKLAVPATGSAA
                     GQIKTMKESDAKHREADEKKLEVEIQKLTNTLIEVKAKANEKNSLFAALTAPKISKLL
                     SERGIEISADNIQVGNGIKELGTHSIKVKFGSKETHF"
     gene            complement(19105..20337)
                     /locus_tag="PLD99_00465"
     CDS             complement(19105..20337)
                     /locus_tag="PLD99_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRH25412.1"
                     /translation="MDLGKMSRTTIVGFVLVALIAFQAGYKRGESVTLISNVKSGLSN
                     VEDGQPTTVDFAPFWKAWSIINKDFVPTGTSTKVATDQDKVWGAIEGMVSSLDDPYTV
                     FFPPVESKQFAADIKGSFSGVGMEVGQEDGIITVISPLKGSPAEAAGLRPGDKILKVD
                     DKDVTKLTVEEAVTYIRGPEGTTVTINVFRQGKTAPFDIKVVRQIINVPVINTEELDG
                     GIFRISLYSFTENSAVLFRSALREFIESDSDKLLLDLRGNPGGYLETAVDMASWFLPS
                     GTPVIREDRGDGKEENVYRSKGYNIFNDSLKMAILVDAGSASASEILAGALSEHGVAT
                     LIGDTTFGKGSVQELLPVTKDTSIKVTIARWLTPNGNSISKQGITPKIVIKRTEEDIK
                     TGRDKQLERAVEFLKTGK"
     gene            20360..21718
                     /gene="cysS"
                     /locus_tag="PLD99_00470"
     CDS             20360..21718
                     /gene="cysS"
                     /locus_tag="PLD99_00470"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRH25413.1"
                     /translation="MISFHNSLTNKVELFVPLKDGEVKMYHCGPTVYDFVHIGNLRSF
                     ILADLIRRYFEYTGSKVTQVMNITDIGHLSGDGDDGEDKMTKALKREGKSLTMDSMQE
                     LANFYTERFKEDTLTLNILPPHHLPKASNHIQEDIELIKELDAKGFVYKTSDGLYFEV
                     SKFPAYGALGGTGAGESRIGEKSEKRDPRDFSLWKFNTELGYEAPWGHGFPGWHIECS
                     AMSQKYLGKTFDIHTGGADLAPIHHNNEIAQSEAACGCEFAHYWLHNEFVILDGSKMA
                     KSDGNIIKLKDIVDKGFSPLAYRYFLLLSHYRTPTNFSFEALEAAQTAYRRLKEVMSG
                     EEGKVNAEYKNKFLSALENDLNTSQALAVVWDLVKDESVPKGDRNSTLLDFDKVLGLN
                     LNQNEFEVKKIPSDIQDLINEREVARKNKDFAKSDELRDLINKKGFVIEDSGDSYKIS
                     KA"
     gene            complement(21715..24363)
                     /locus_tag="PLD99_00475"
     CDS             complement(21715..24363)
                     /locus_tag="PLD99_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HRH25414.1"
                     /translation="MSEAPRFNSVEEELSYLRSKVESYKDNSTEVKRTIINTHQELPV
                     QVRQSTDVKQSLETLSNKPTDSQLDHMMDISRTKGVMHALSVAEKLEDWKHEDDFHDY
                     LVELVRGGFKPKDVKESGPMYRALNMTLFEIQMPDIGSDGTKDRDLKSVISSMEQFYA
                     GMISVSEEATRGKNYFVLEVANSEGSEEANFFVSVPNEKRDLFEKHLRSVFPNVRLVE
                     RSRDYNIFSEHAFTTGAVAELNQSSAFPLKTYDEFDHDPLSSLLQSFSKIPRDGSGAA
                     IQIIFNPQGDAYTKKYTFAKKRIEKGHKSSDVLAERSILGEVVHGLSEFFGGKKIDPT
                     KVDAEALKNIDKKLLSPIVATSIRIAVSAQNEKEAKSIVSSIASSFNQFEIPNVNRLN
                     WREARGKKFFRNFTFRLWNEAEIMPLNLKELTGLLHFHTTSLSSSAQLKTSKTSEAQA
                     PLSMSLGGTVLGTNHFQGHDRQVHISYEDRLRHIYTIGQTGTGKSTLLKNIIIQDIKA
                     GNGVCMIDPHGQDVQDVLASIPPERMEDVIYFDPASTERPMALNMLEYDKDKPEQKTF
                     VVNELFSIFQKLYGKVPESMGPMFEQYFRNATMLVIDDPSSGSTLLDVSRVLSNKEFR
                     TMKLEKCTNPVVLQFWREVADKAGGEAALANIVPYITSKFDVFLANDIMRPIIAQEKS
                     SINFREIMDNRKILLVNLSKGRLGDVNANLIGLILVGKILMAALSRSGSTGFPPFYLH
                     IDEFQNITTDSIATILSEARKYKLSLTVAHQFIAQLEESIKNAVFGNVGSIIAFRVGA
                     DDAEYLEKQFAPVFTAKDLMNIDNRNAFLKLLVDGRPEKPFSMETMSPTEGNRELIDI
                     LAEKTKLKYGRNREEVEKEILAKYGN"
     gene            complement(24381..25247)
                     /locus_tag="PLD99_00480"
     CDS             complement(24381..25247)
                     /locus_tag="PLD99_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25415.1"
                     /translation="MEKFVPEGLERADISVIHGRAEKVMDKARIKEKDFVNPYGQETI
                     DRDLETVRRHERDFDHNTNEYTKEQKAVADIFEALVLENGELNDWFGPNAITHKTSKY
                     DDYENGVDAIIEFQSEMAGAASFLGLAADVSFSADTSRKFDRLKEGILKGRLPQVKYF
                     NSSDGYFHGKLSMIPEVVIGASKQTVLELAELWQFKRNKELATHRIQIMILNQMEEQM
                     EVFARFAELNGQDEIARLYRDRLQIVRDILEGKRDIAKEAVKIKSKDLVHEGIMDFLK
                     HWKLEMEREASI"
     gene            25341..26756
                     /gene="dnaB"
                     /locus_tag="PLD99_00485"
     CDS             25341..26756
                     /gene="dnaB"
                     /locus_tag="PLD99_00485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRH25416.1"
                     /translation="MQESSLASLKIRVPPHNLEAEMALLGSVMLRPDAIYEIMDISSP
                     ASFYFEKHRTIFEAMLELFAKREPIDILSLSSKLKEKGLLERVGGASFLTELTTTVPS
                     SANIKHYAEIVRKKFMMRELIDASEYISHLGFNESGELENILDQAEKKIFDITQHFSS
                     TENFKDLTPLLQEAFERFDKLSSAEHELRGVPTGFKSLDDILGGFQKSDLIILAARPS
                     VGKTAFALDVARRTASLHQTPIGIFSLEMSADQLVDRIVSAQSKVDASVIRKGVRTQQ
                     GSWRDDIFKNISTALDDLSRTPIYIDDQSGNTIMKMRSAARKLKIEKGLQLLVVDYLQ
                     LMAPTNARAGDNMVQQITEISRGLKHMARELQIPVLALSQLSRAVETRGGKPKLSDLR
                     DSGSIEQDADLVMFIHADEDGIRDESGKIKEVQRKSLIIAKHRNGPLGEVVLDYHTRN
                     NTFVEIDYSNYGKAEEEFKSF"
     gene            complement(26753..27145)
                     /locus_tag="PLD99_00490"
     CDS             complement(26753..27145)
                     /locus_tag="PLD99_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25417.1"
                     /translation="MENENDSKIKNIKRLSTLINLLAVALLCFSVLGFIVALIMNVGS
                     GPYPKAFIIMPLTTFLLGISLPRVSYGVKMLKRKSLNMLLVILPIVLSVLVIMTLSLP
                     HLILVFLALMIICIISSVYLWSQRKLFT"
     gene            27184..>27617
                     /locus_tag="PLD99_00495"
     CDS             27184..>27617
                     /locus_tag="PLD99_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25418.1"
                     /translation="MNSIDTLAEIFAHFPGIGPRQAKRFVYYLLSRDKSTVDSFVRAV
                     EKLKQETLQCTECKRYFARNGSDKPICSVCSDSHRDKSLLMIVPRDADFDAVEKSGSY
                     KGYYFILGGSVPILEKEPEKRIRLSELSTKINGNKSVLKEVIL"
     assembly_gap    27618..27717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27718..28154)
                     /gene="rplU"
                     /locus_tag="PLD99_00500"
     CDS             complement(<27718..28154)
                     /gene="rplU"
                     /locus_tag="PLD99_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRH25419.1"
                     /translation="MAEAKTAKKSAPKAKAPKAKVEGAKEAPKTAQKASRRPMAVSKT
                     NFAVIMTGGKQYRVHEGQMLKIEKILGDHKVGDALVFKEVLMKNEGDKMTLGNPTIGT
                     ASVHSTIIDISRYKTVDVIHYKQKSKYFKKYGHRQPYFEVRIDS"
     gene            complement(28162..29220)
                     /locus_tag="PLD99_00505"
     CDS             complement(28162..29220)
                     /locus_tag="PLD99_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRH25420.1"
                     /translation="MNWIIVLLVALFALNLGIFFVFVKKKEGGEKSEGMNLLLQQLNE
                     LSRTVDQKMTEGNRLMHESVKTQFGESAKLIREVTEGLTKLDETNRQVVSFADQLESL
                     QDILKNPKQRGILGEYYLETLLKNVLPPGSYEMQYELGITDDGARLVPDAVVFVKEKI
                     IPVDSKFSLENYNRLVEEKDPVEKAKLEKLFVSDLKMRIQETAKYIQPSKGTMDFAFM
                     FIPHEAIYYDLIVNKIGALQEETESLIQRAASKYKVIIVSPTSFLAYLQTVMQGLKAL
                     QIEEKAQEIIKKVEDLGRHIKTYDEYYKKLGTSLGTVVNHYNTGQKELGKIDKDVLRI
                     TGSSSGIDTLTIDRPNVE"
     gene            29236..29691
                     /locus_tag="PLD99_00510"
     CDS             29236..29691
                     /locus_tag="PLD99_00510"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRH25421.1"
                     /translation="MSLQTDIKSGIKDAMIAKDAVRLSVLRGLSAAFTNELVAKKMKP
                     DGELSDEDALAVISKEARKRKDSIEQFEKGGRPELAEDEKAELAVLSTFLPEQMSYEA
                     VLEFVKAKQVEMGATDKAKAGQFMGSVMKDLKGKADGQVVKAAIDSLFT"
     gene            29855..30262
                     /locus_tag="PLD99_00515"
     CDS             29855..30262
                     /locus_tag="PLD99_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25422.1"
                     /translation="MATKTEEKVEETFSKELLFDFSEAFKRNKELSPRRSFYLVFLDT
                     CREFRIFGERQKKFFEVFAIYRATVRIPEGIRESAYFLHEEAPHVVMAPVVVPVTPPV
                     KKVRSLPNISVRSGDTHDRHESQLPRGDRNDDD"
BASE COUNT         8410 a         6929 c         6627 g         8011 t
ORIGIN      
        1 ctcgctatag gactagaccc aaagaaggta actctctact accagtcaga tgtacctcat
       61 gtgacagagc tatcatggat cttctcttgc cttactacta tgcccgagct tatgcgtgcg
      121 catgccttca aagacaagga ggcgaagaat gccgacatca atgtgggtac cttcaactac
      181 ccactactca tggcagcaga catccttata catgatgcag acgtggtccc cgtaggtaag
      241 gatcagaagc aacacataga aataactcgt gaggtggcac gcgacttcaa taacacttat
      301 ggtgagacgt ttaaagaacc caaagaactt gtactagggg ctgtggaaac agtaccagga
      361 actgatggac agaagatgag caagagctat ggcaacacca tacctctctt tgctactgat
      421 gaggaaatag aaaaagcagt aatgagtatt gttactgatt catcgggaga gtatccagaa
      481 aatgttttca aaatgcatca attatttaga acaaacgacg agctagacca gatttataca
      541 gataataaag gtcaatacaa aaagctcaag gaattgcttg ccgaggatat caagaacttt
      601 ataagaccaa tgcgagagag gcgagccgag tgggcgggga agatggacga ggtagacaat
      661 atagtggccg agggtggcaa gaggatgagg aaagtggtgc acgatagaat gatcgaggtt
      721 agaaacaaag taggattaat acacaatgac taaaagaaca tttatcaagg acttgagtgg
      781 aaagaaaggc gaagaagtca gtatctctgg ctgggtagat gtgcgtcgcg accaaggcaa
      841 aatgatcttc ctcgatatcc gagacatgac gggcaaggtc cagtctgtgg tactccctaa
      901 ccacacagag gctctagaag tagccaaaca actccgctca gagtgggtgg tgaggattgt
      961 aggtaaggta aacgcccgtc cagagaagaa tataaatccc gaggtagcaa acggagacat
     1021 agagctcgaa gtcacgagcg ttgaggtact cgccaaggct gagcccctac cattcgacat
     1081 gtctatagac ggttacaacc tagaccttgt gacagaactc gacaacagag ctttggtact
     1141 aaggcacccc aaggttcaag ctgtctttaa ggttcaggaa actattatcg atgcctttag
     1201 agagcatctt aagaacaata tgttcttcga gttccaagca ccagctatta ctccagctgt
     1261 ggcagaagga ggtagtgagg tcttccaggt agattacttc gacaagaaag cttacttgac
     1321 tcaatcacca caactctaca agcagattgt aatgtcagcc ttcgaacgag ttttctccgt
     1381 taacaagatc ttccgcgctg agccgagtgc taccacaaga cacctaaccg aagtagtctc
     1441 actggatgct gaaatgggat tcattgaaag ttgggaggat attcgcgaca tggcagagag
     1501 cacagtaaga cacatcttga agagagtgtc agaggataat gcagaacacc tcaagcttct
     1561 caaggtcgag ttacctacaa tgatagagaa gactccaacc atatccctaa ctgaggctca
     1621 ggaaaaaata tttaaggaat ttggtcgtga tgtacgaggt gagaaagacc tcaatgctca
     1681 agacgagcga gagctatgcg agattatcaa gaaggagacg ggatcagact ttgtctttgt
     1741 ctatggctac ccaacaagga agaagccgtt ttatgtgtat cccaaccctg agaatcctga
     1801 attcaacgaa ggtgtggacc tactctgccg aggacttgag tggatgtcag gtggcagaag
     1861 aatcaacaaa tacgaccaat tacttgagca tgtaaaggag tggaagatgg atccaaagaa
     1921 gatcaagatg ttcttagagg ctttcaagta cggggtgcca cctgaaggcg gtttcgccct
     1981 cggagcagag agagtcacca tgcagctatt gggtcttaag aatgtgcgag aggcatcgat
     2041 gttcccaagg gacatggacc gtatcgacga gagactatct gaaacaaact aacatggagg
     2101 ggtttggcta ttcagttaag acggaggcat tcaccgggcc actagatctt ttgctcgacc
     2161 taatagagaa gcgaaagctt tttgtgaacg atgtctcgct ctcacaagtt actgacgact
     2221 tcgttaagta tattgagacg catgaagaat tccccataag cgagagtgct gagttcatcc
     2281 tcgttgcctc gactctcatg cttatcaaaa gtcgttctct cttgccttcg ttaccactaa
     2341 ctgaggaaga gactgcatct atagaggacc tagaggaccg cctgaagctt taccagaagg
     2401 tcaaggagtt aacagtcgaa cttaagaagc tctacggaca gaaaattata tttgagaagg
     2461 aacccaagaa aagcgagata atcgtcttct ctccggactc gaagaccgat attgataacc
     2521 tctcaaatgc cttgatgcat ctcttgaata ccttgcccaa gaaggaggct ttacccaaga
     2581 ccacagtaaa gaagatcatc accttggagc agatgataga gaagttatca gaaaggatag
     2641 ctaaaggtat gcgaatgaag ttcagcgaag tgtatagtaa ggaggagaaa gtaaacacca
     2701 ttgtcggctt cctcgcagtg cttgagctcg ttaaaagagg cgtgctcagg gtcaaccaag
     2761 aggttaaaca tggtgagata gagatggaga cagacaaaat aggtgtccca agttatattt
     2821 aaaacccatg acactagaat caaagataga agccatattg ttttacaaaa atgagccttt
     2881 gacacacaaa gagctctcta aaatacttga ggtagatgta gacacagtaa gaaatgcact
     2941 aagcaacctt aaaaaaagtt atgagaataa gggcatagtg ttgattgaga ctagtgactc
     3001 cgtcgctctc ggtactcacc cagagttaag ctctatgttt gaaaaaatgc agaaagaaga
     3061 gttgagtaag gaacttggtc gcgcgggtct tgagactctc tctataatcc tatacaaggg
     3121 gccagtagcc cgtcgtgaga tagatcacat aaggggtgta aactctggct tcatagtaag
     3181 gaacctcctt atccgaggtc ttatagagcg cacagaatca ggaatggggg agagaagttt
     3241 ctcctacagg cccacactcg ccctcctcaa gcacttggga gtaacgagtg tgaaggattt
     3301 gccagaatac ggagtagcac tacaaaagat gaatacattt attgccacgg aagaaaaaga
     3361 tgttaacgaa taaacaagaa atagcttttt tctccttcct tggagtggtc cttgtgttcc
     3421 tacttacctt cacctacata cttggatcca agagtgtaga agggcagaaa gtggttgtga
     3481 agaagagtac cccgctacac attggccgag tagacataga ggccaaggcc gcttatgtat
     3541 acgacactct aagtggggaa gtgctttatg ctaagaatga gaatgagcgc ctacctctag
     3601 cttctctcac caaggtcatg agcgctcttg ttgctagtga agaggttccg agctaccgca
     3661 ccatagcgat atcagaaagt tctacaaaaa cagacgggga tgcggggcta gttccaggag
     3721 agcattggac tcttaaggac ctgctcgact tctctctagt aagctctgct aacgacggta
     3781 tacgagctat tgcacttgca gttggtgcgc ttgatcaaaa caatgcaaat gatgagagcc
     3841 aagtaagtga cttcgtcctc aagatgaacg acctagcgag tcaaatcggt atgaagaaca
     3901 cttattactt gaatgacact ggcctagacg aatccaaaga gcaaggaggt gcttacggca
     3961 gtgccaagga tcaagccata ctctttgaat acattttgaa gaagaatcca accctactag
     4021 cgagcaccaa atcctcaata ttaagaatta gaaatgttga gggtcagata cacactgctc
     4081 gtaacactag ttctatcgtg aacgacattc caggcatcat cgcctcgaaa accggcttca
     4141 ccgacctcgc aggaggtaac ctgattatcg ccttcgaccc tgaaattggt cgccctatag
     4201 taatctcatt actcggctcg tctgaagaag ggaggttcag agatatgatg gtcttggtaa
     4261 aagccagttt agccagcgtg gaaaatagtg taatatctac agaatgattg tcgatgtagt
     4321 caaaatcttt ataccctcga tcctcgcttt tgtacttggc ttagtcatca caccctttgt
     4381 ttctaaaatt ttgataaaga ataggatgtg gaagaaaaag agtgttgttg tttcgactga
     4441 cggcagcggc gcacccatta cccaggcact ccatggcgat gaagaaagaa agactccaag
     4501 aatgggtggt atcgtcatct gggcaagtgc tctcattacc atcctagtat tctggcttgg
     4561 cgccgaagta gatggaccag tgtttgataa attgaacttc ctctcgagga accaaacttg
     4621 gcttccgcta tttactctca tcggtggagc attgattggt cttatcgacg actattggac
     4681 agtaagcgag atctatgacc agaaagctgg tggactctcc ttcaaaaagc gtatcgccat
     4741 ggttaccata gttgctctca ttggtgcttg gtggttctac tacaagctcg agatgacaag
     4801 tatcattgtg ccattcctcg gagtagtcga cattggctgg ttcttcatcc cattcttcgt
     4861 tcttgtgatg cttggtactt actctggtgg agtgatagac ggattagacg gactctcggg
     4921 tggagtattc gctattatct ttggcaccta cggggtcatt gcctttcttg gtaaccaaat
     4981 agatctatcg acattctgct tcgtacttat gggaggactc ttggccttcc tctggtacaa
     5041 catcccacct gcactcttct tcatgtcaga gacggggatc atggcactca ccatgactct
     5101 tgctgtcgtt gcttttctta ctcagcaagt tcttatactc ccacttattg ctttcccact
     5161 agtagtatcc tccgcatcaa gctcaataca gctattatca aagagattta gaggagggaa
     5221 gaaggtcttc ttggtagctc cactacacca ccacttccaa gcacttggct ggccagcatc
     5281 aacagtcgtt atgcgttact ggattgtcgg cgtattcgtc agcctcctcg gtctcgtcat
     5341 cgtccttttg ggttaaattt tagcttctct ctgatataat ttgcctcatg tccttcaaag
     5401 agtggcgagt tgataaacct tttttcttcg cagtaattat tttggccatt gcgggatttg
     5461 ttagtttcta ctctgcctca ctcggcctcc tcgccaagtc gagcgagcaa tactcttctg
     5521 ttaccttctc ccaaacagtt cttgggcttt gcctcggatc agttgccatg ctagttgcta
     5581 taagagctga ctataagaag tggaagaagt atgctttcta cctactgctc tttgcaatct
     5641 ttttaaatat tttagtactt atccctcagg tgggttttgc gcacaatggg gcaagacgct
     5701 ggatcgagat cggtggacta accttccaac catctgagat actcaagctt gctttcgtta
     5761 tctatctagc agcctgggcc tcgaaggtga aggagaagat gggcgagtac aaggccggct
     5821 tcatcccgct agttgtgcta ttagtacttt gcgggactct ccttcttatg caacccgata
     5881 ctgacacttt tgtcatgcta gctactgcag gcgtagcaat ctatattgtc ggtggtggta
     5941 gatggcgtga cttgatgatc ctaatccttg tcggagtttt gggcataaca cttcttgcag
     6001 catttaggcc gtatgtaaga caaagaatct ttacctactt caatcctcaa gctgaggcgc
     6061 agggttcagg ctaccagata caacaatctc tcattgctat tggctctgga gggttcttcg
     6121 gcaaaggatt cggtcagagt gtgcagaagt tcgccttctt gcccgaacct atgggagact
     6181 caatctttgc ggtccaagct gaggagttcg gcttcgtagg ggcatcctca ctcatcctcc
     6241 tctttgtctt ttttgctgtc cgaggcctta aaattgcatc aaacacacca gaccccttcg
     6301 gtagactggt ggtcgtcggg cttgttataa tgattactgc tcaggcgttt gtaaacattg
     6361 gggcaatgtt aagtattttg cccctttcgg gtattacatt gccctttgtg agccaaggag
     6421 gaacctcgct attctttacc ttacttgagg cggggatcat cctctcgata tctaaatcag
     6481 ctactttgat cccaaagagg aagtagtgca acttccaaaa taaacatgag aatccttttt
     6541 acaggcggcg gaactggtgg acacttctat cctattattg ctgttgcaga agctatccga
     6601 gaagtagcca aggagaaaaa actcctacct ccagagctct actacttcgc tcctacacca
     6661 tacaacgaga gtatcctctt cgaacaaagt attacttata agaaggtcca ggctggtaag
     6721 atgcgtagat acttttcgat ccttaatttc ttcgacctat ggagaacggc ttggggagta
     6781 cttagtgcca tatacaaagt ttatagactc tacccagatg tcattttcgg taagggcgga
     6841 tacgcgagct tccccgtact tttcgcagca aagatactcc acatacctgt cgtcatacac
     6901 gagtcagacg ctgtaccggg cagagccaac ctttgggccg gtaagtttgc aaacaaaatt
     6961 gccgtatctt gggccgaagc agccaagtac tttccatctg gcaaggtcgc acataccggc
     7021 aacccagtaa ggggtgacat caggcaaaga aagactgagg gtggtaaaga gtttatgaaa
     7081 gtggaggaag gtatccccac agtactggtc ctcggaggat cacaaggagc agaggcaatc
     7141 aacgaagtag ttgttgaggc actcccagag cttatcaagg attactcggt aatacaccag
     7201 acaggcaaga ggaatattac ctcttcaaag accactgccg atgtcatatt gagtaagtcc
     7261 gccaacaagg acagatataa accagttgac taccttaacg gtctcgaact tcgcatggct
     7321 gcgggtgcag cagacatagt tatctctcgt gcaggatcga caatttttga gatcgctgcc
     7381 tggggagtgc caagtatcat tgtacctatc aaagactcaa acggtgacca tcaaagagca
     7441 aatgccttct cttacgcaag agcaggtgcc tgcgaggtgg tagaggagga taacctaact
     7501 gcaaacatcc ttgttgcaga ggtaagaagg atcctcagta gtaccgagct aagtgacaag
     7561 atgaaagctg gggccaaagc cttcgacaga gcagatgcat caaagaaagt tgcagaagaa
     7621 attctcaaaa tcgcactctc tcacgaattc gaaaactaat atgaatattt taaataaatt
     7681 atttggacga aataaagtag ttgttcgcat ggcaccctct cctacaggac ctctacacgt
     7741 tggtactgcc cgtaccacac tcttcaacta tctatatgcg aaaaaaatga gtggtaagtt
     7801 cattatgcgt atcgaagata cagataaaga gcgttcaact aaagagtttg aacaaaatat
     7861 tttagacgga cttgattggc ttggcctaaa gtacgatgaa ttttatagac agtctgaaag
     7921 aacagatgta tatgtgtctt atgtaaggaa gatgatcaaa gagggtactg cttatatatc
     7981 aaaggaaact ccaaaggaac caggggatag ggctgaggtg gtaaggttca aaaatccaaa
     8041 caagaaagtt attttcgaag acctcattcg tgacaaggta gagttcgaca ctactgaact
     8101 tggtgatttc gtcatagcca agtcacctga ggaaccgcta taccacctgg ctgttgtagt
     8161 agacgaccac gagatgggag tcactcatgt tatacgaggt gaggaccaca tatcgaacac
     8221 cccaaggcaa atccttatac aagaggctat aggggccaag aggcccatat acgcccactt
     8281 accactgatg ctcgacgccg ataggcagaa gctctctaag aggaagcacg gcgaggcagt
     8341 atctgtggac ttctacaaga agtcagggat tttacccgag gccctcctaa acttcttagc
     8401 cctcattggc tggaacccag gtactgagca agaggtcttt accttaaagg aactttgcga
     8461 ggtgtttgat atcaagaagg tccagaaagg tggagcagtc ttcaatgaaa agaaactcga
     8521 gtggttcaac aaggaacata tcaagctctt gccagaggaa gaacagttca aaattttgca
     8581 gaagtctata gagcacgagg attattttag tcgtgcacca gatttagata tcacaaaaat
     8641 taattggaag actgtcccag ttgctgaaac aaaaatgcat ttggaaaaag ttttaatgat
     8701 catcgacgac aaagaccaga tcatgaagta cgcagaagaa gcagggaagg gtaatgtact
     8761 ctggccactc cgctttgccc tctctggcaa ggagaagtct ccagacccat tcagtcttat
     8821 aaggattatt ggcatagatg aatcaagaag aagggtggag aacgctatta aacttctcta
     8881 atgaaaaagt ttttattctt acttgtaata cttttctgct tctttccgtc tcctaccacc
     8941 gcacaaacca actcagacat actagacaag atagaggaaa cgaaaagaga gcgtgacaaa
     9001 ctgctagaag agcagaagaa gatccaggct gcgttaaacg aaatatctaa gcaaggccag
     9061 accctccaaa ctactgttaa aagcctagac actacgagaa gtaagcttgc taacgatctc
     9121 aaacttacac aaacaaacat taatgcctct aatcttacta tcaaaaaact tgaaggcgac
     9181 ataggtacca atgagtcaga gatagaaacc cacaaaagtg ctatcaaaga atcactgcaa
     9241 caaatagcag cttatgataa acagagtttt gtcatgggtc tcctaaccta cgactcactc
     9301 gatgaggtgt ggtctgacac cgtaagtatc tgggacctcc aggagaagat caatgagcgt
     9361 ataaatgtac tagaaaaaac acagatcaaa ttgatagaga acaaggttgc caaagagtca
     9421 cagaagaagc aactcgttag tctgtcctca caactaagtg gtcagaagcg agtagctgat
     9481 gagactcgta ctgcccaagc cctactcctt tcagccacca aaagcaaaga agcagagtat
     9541 caaaagatgc tcgcggagaa caaagctagg gaagaagaat tcgaacgact actcttccag
     9601 ttcgaatctg ctttaacagc ctctgatgct tcagagagac caacaggggg taagggctca
     9661 ttagcatggc cactcgacaa tgtattcatc acacagcaat ttggcagaac gagtgcatca
     9721 acgagactct atgtttctgg tactcacaac ggcatagact tcagagcaac tgtggggaca
     9781 agagtaaaga gtgtgcgaga cggagtagtg tctggtgtag gcaacaccga tgatcaaaga
     9841 ggttgttact cgtatggtcg atggatactt ataaggcaca acaacggtct atcttcactc
     9901 tacacacatc tctccgggtc tatagtgtca gctgggcagg aggtaaccga gggtcaggta
     9961 ataggctaca ccggcgggca gccgaggcaa tatggttctg gctactctac tggtccgcac
    10021 ctacacttcg gtctcttcgt cactgctggt gtgcaggtcc taccatactc atcaagcaag
    10081 aactgccaaa atgtcagtat ccctcttgca aaccccaagg actatctaga cccccttggc
    10141 tatctaccca acctctagta gcattaagct atgaaagaaa aggggttcgt tatgatcatt
    10201 gttatagtcc tcattgcctt agtactcttg aagtacttcc tcaattggga tatattcgat
    10261 gccgcctcga cggagcaggg taagtcgaca atcctctaca ttagagatgt tgtgaatctg
    10321 gtatggtcct atatatctgc tccagtaata ttcatttggc agaggattgt ctggccaata
    10381 gtagacattg catggaacaa cttcaacaac tttattgatt ggggaaggaa taactacaac
    10441 ccatcaaatt aacaaattag atatgaaaaa agttttattc ttccttctac taccacttta
    10501 tgctccagct atattcttcc tcaacaacac catgaagtgg tgggagggat tactcgatta
    10561 gcatgagtac aaatacttta aaatttatcc agatagtact cgcgattctt atttgtattg
    10621 gtcttattct tatattcacc aaaggtctat gggtccccaa ggtggtcaag ttccagctag
    10681 agcaagaggg gatatacagc tactacatag aggcaacaga caactttgac atttagcagt
    10741 tttgtgatat tgttctagaa agacacccaa acggagtaac agatgacgag agacaagatg
    10801 gttgactatc ttccattctc agggcctgat cacttcggcc agagcgatgt ggaagaagcc
    10861 aagagggtct acatcgccac tcacccgcgc ggctcagaaa acccttcaat cgtagtggtc
    10921 actggcaaac cagaggacta cactgatgaa gagatcgaga agctcttcaa attcgcgaag
    10981 cagaagacag cagagtggat caccaacgca agcgggagac cgcctatcat gatcgtcatc
    11041 ggtaaggttt cccccaggtc tacaggatca tgggtgagga agagggtcac ctggactggt
    11101 ggcaacttct tctctcccac gctcgacgaa gcgatccagc gcatggcctc cgcgtannnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttg acaagccctc cccttgtcgg
    11281 tagggtgggt gtagtcggtt tgtactttca ccctcatgtg agacatccaa tgccccttgt
    11341 gaccatttat gtaaacgatc agtttcttga acccaatgtg agaatggccc ttgcagtgag
    11401 agtccatagg gagattgaat cgatagttaa gaagagcttg gatgtggaca ccgttgtagt
    11461 gcagcagatc tctgtctcac atctctcttc gatattcatc gacataacct gcaaccaccg
    11521 tgagactcga gaagctaatc tgaacgaaag acgagacatg atcgcggagt ggctgagggg
    11581 tatcatggaa gaggctggaa tgaggactgg tgtagttcat cttcgcatag tactggctcc
    11641 agtttcgtat tgtgaacttg tagtgcaagc gtgacccatt cgtgggtctt tttcttatcc
    11701 actgctttag aacgattgtt ctaaatgggt gatatactat tggtatggaa ttagaagtag
    11761 ccaagattgc cgacttttat agagaggaga agaggatgcc gagttactcg gaactccaaa
    11821 gactcttggg ctacaaaagc aagtctgcgg tgcattactt cgcggacaaa cttattaaga
    11881 agggattcat cgacaaagat gacaccggca gactcttacc aaagaacttg ggagagatca
    11941 tggtcctagg agtggtggaa gccgggttcc ccacatcagc atctgaagaa ctcctagaca
    12001 ctatgagcct agacgagtgg ctcgtggaga agaaagaagc gacctacatg cttacagtta
    12061 agggcgagag catgatcaat gcggggatac tcccaggaga taccgtgcta gtagaacgag
    12121 gggtgacacc aaagcttggc gacatagtga tagccgaggt cgatggcgaa tacactatga
    12181 agtactacag gatgaagaat aaaaaagttt atctagaagc agcgaacgac aagttcgatg
    12241 atatctaccc cgaggaatca ctctctatac ccgcggtcgt aagagcagta ataagaaagt
    12301 ataagaaata actaataatg caaagagcga tactacacat agatggagac tccttcttcg
    12361 cctcatgtga ggtggcacta gaccccaaac tcaaaggtaa gccagtggtg acaggccacg
    12421 agcgggggat agccaccgct atgagcaaag aagcaaaggc tctgggtata agccggggca
    12481 tgcccgtatt tatgatcaag aagctctatc cgagcgctgt catagtcgag tcgcactatc
    12541 acatatatgg tatgttcgct caaaggatgt acgatattgt gcgacgttac accgatgtag
    12601 tcgaggagta ctctatagac gaatgctttg ccgatattac acatcttgga aagccaggca
    12661 caacctacaa gagcatcgca gagtctatac ggaaggattt gaagaaggag ctaggcatga
    12721 catttagcat cggacttgga gagactaagg tcttggccaa agtagctagc aagtgggaga
    12781 aaccagatgg gctgactatt attaataaag acaatattca aagtatctca agcaagctcc
    12841 aaataggtag tgtctggggt atcggtccat cgagtactgt agaacttaat cgtcataaca
    12901 taagaaccgt acatgacttt gtatcaaggc cacagggtta tatagaatct aacttctcta
    12961 gacctatagt agagacttgg cacgagcttc gtggcacaca ggtgcatcat gtaagtcaaa
    13021 gtgacgagtc caccgatgat caaaagagtg ttcagtccac caaatctttt ggtaaagcat
    13081 caaaggacaa gaagttcttg ctctctgaac tatctaggaa tgtagaaaga gcttctgcac
    13141 acgcaagaca acttggactt ataagcaaac atatatatat cttcctcaaa accaaagagt
    13201 ttagatacaa acgagttgat gtacaacttg cacaagctac agcctcgccg acgctcataa
    13261 tgaatgaagt tcttaaggtt tttgactcaa tatacgatga agagacctta tatagggcaa
    13321 ctggggtcac tttggccaat ttggtacccg aaaacttaat ccaagaggat ttgtttggag
    13381 aaagcgagaa gaaaagtatt tggaaaaata tttttgaaat tacagatcag ctagatagga
    13441 gatacgggag tagaacgatg gccctggcgt cgagcctagg atcaatgaag gtgaggggtt
    13501 ataaaccaag acgcctttta aatattccat ccatggggga gaccctttag cttaaccgta
    13561 gtgtcgcaaa agatacattg tgcgacgcat atagcctaag aacaaacctt aataaagcct
    13621 actcccctat gaattagggt aatacaacaa agccccgctc acgcggggct ttgtgactct
    13681 caagaattag tttttagcta tgttctttga agtagtgttg attgcgagat cctcggactt
    13741 gtcccaacaa ggggatgacg agttccatgg tctactcccc tccctctcat agaggtgtcg
    13801 ggcgaaggcc acattaccgt cgatagtctc taagtcgaga ccaagtgcgc ttgcctcttt
    13861 ggcatgatac atctcattga tctgcatcac accaacgtcg taacgattca cttttcctct
    13921 ccttggctca ccattctttt cgaagtggcg gaaggatgac tcacagaagg caatcttctt
    13981 gagttcggga atatcggaga aataatcttc gatgaatcgc tccacattgg caggatccgt
    14041 aatcggcata tagtcttcgg actttatctc tttggggtct gttgaggcca ctctgtgtat
    14101 ctccgagatc ggactgacct tatggatcat agtaggagta ggctgacccg atagagcaag
    14161 ggcgagacta agcgatgcga gctgtaaaat aactgaaggt taaactgtta agtaaagggt
    14221 ctgtgactta tcattgccct ttagaaacga aaatcagcgc actggctgat tgggtatata
    14281 ctagcacctt ttgactcgct tgtcaatggt ttttatggta taatttggta tatcgtggct
    14341 taataaagcc atattttagc tctaagtact ctgctatttt agatggagta cgtcaaatag
    14401 gagggtctgt cccctacttt cgttttactt ttgggcacta agtactatct cgccctctga
    14461 aagcccttct acaacctcta caaagccatc cttacccagg attccgactt gtatttctct
    14521 ttcttcctcg ccttctttgg tcttaacgat tacaaatctc ttacctgacc ttctctcaac
    14581 agcaaaggcg gggatacgga gcacatcctc tttcctctcg gtgctgatag ccaagctaga
    14641 tgttagacca gtcttgatat tttcaggagt gttacctacg aagactactc ggacctcata
    14701 gttcaccacg ccgtcaacaa gcttgtcccc cggctcaaca taaagcaccc tgcctagtat
    14761 tgtctcacca gggaaggcgt ctagagagat cttcactgtg ttgtctgctt cgatcttgcc
    14821 aatgttcacc tccgatatat cagcaactat ttctagattc ttgtcggcca caactgaggt
    14881 ggtctgcatg cccgatgtta ctatctcccc caccttagcg tcctgcttgg tcactacacc
    14941 agaaattgga gacttgagca ctgtcttgct tagctcagac tgcaaagatt ctacatcagc
    15001 tcgagtggcc tccacccttg actcctcttg gagcacttcg tcaaagcctg atccaaaaga
    15061 cacttcgttt ggcgcatcca agtagctaga cttagctgtt aaaagttcgg agagcgaggt
    15121 attgagcgct gtacgagcag tagtcatcga ggacttatag cccgcaataa tactttcata
    15181 tgactggtca taacccttga tagtattgag aatgtaagaa aggtccgcca agaaattctt
    15241 tactctatct atattcttct ctgttagtgc aagttcacca tctaaaccgt ttgtgctggt
    15301 ctttgcacca tttatagaca agtcccatgt agcaaatata tcgtttaaag cacctctctc
    15361 gacactaata tttcttgcca aggtgtcatc tatataaaac tttatgtcat taccatttga
    15421 tacatgactg atctcgagtt taggagacga actcttaggc gaccagaaga agcggtctgc
    15481 agtgtttagc actgagtctc tagcgtctgt atatgcatca attagagcta tatatagttt
    15541 gttgtaggca ttagagtagt ctgaggaaga agttcttttt gccttctcga gattgataag
    15601 cgcttcgtta agagaggcgt tggccttctt gatatcggcc aagaggtcgc tctggtctag
    15661 cactacaaga gtcgcgccct cttctaccac gtcacccaca ttggcgttta ccatcaccac
    15721 cttgccactt ctttcgaagc ctagatctac ctcgctcgca gagcgcacct tacctgtggc
    15781 aacaacctct tgctccacta cacctctctc aactacagac tcggtctttg gcttatcgcc
    15841 tcccgtgaac aaaaagagtc caataattac tacaagaaca aaaatgctta tttgaagttt
    15901 agttctcgct tttataaatg atttaatttt ttgcatggtg gtgtctgtgt ttttcatcgc
    15961 ttaggatctt accgtcggaa agcgtgatga ctctgtcagt cattttcgca tattctggtt
    16021 catgagtcac catcaaaatg gtctgacctt ctttgtttaa ctccttaaag ttctctaaaa
    16081 ctaattttga tgactctgaa tctagattcg ctgttggctc atcagcaaat ataatttttg
    16141 gctcatgtgc tattgccctt gctattgata ctctttgctg ctgaccacca gaaagcatcg
    16201 atggtaggtg gtctagtcta tcaccgagac caatcctttc cagtgactta acagccttct
    16261 tcttggcttc atcactcgtg agaccctgca tcataagtgg gatgagcaca ttctcaaacg
    16321 ccgttagctc tggtattagt gcataatcct gaaagatgta tcctaaagaa ttaagtctga
    16381 gagacgttct agcaacctca gagagatctg ttatatcctc accatctata attatcctac
    16441 cgtcactcgg atgatcaaga agtcctagtt gatacataag tgtgctctta cccgagcctg
    16501 atctgcctgt aattgcgacg aactctccgc tttctacctt aaatgaaaca tcattaatag
    16561 cgtaaataaa atcatctccg ctcttaaact tttttactag tgactttgct tcaatcattt
    16621 tgttttgcct aatatagcgt ctaaggtgtt ctgcttgacc accaagcgcg ctggaataaa
    16681 tccagcaata attgttgcga taagtagtag caatgctctg atcatcgttg ttccaagctc
    16741 agccaccaag ataccatcag aaaatgggaa cttaattgga tgagcatcaa tgaacggaac
    16801 caagaatcca taaagaataa cgagacccgc agctatacca agtaaggcat aaaatatcgc
    16861 ctgcattaca taggccatct cgatagcgct cgaatcgaca ccgataccct taagtattcc
    16921 aatatacttc ctcctagtga tagcgttgat gaagatgaca ataaatatcg ttatagaagc
    16981 caccaccagg ccaattgagc ctatagcatt accgagaatg ctaaaggtgt tttgaatatc
    17041 tagcagaaac tttggcaatg actcctccca tgtaagcact cttgcataat cacctgcccc
    17101 agtacgtaat atactctctt tcatcaaatt catatccaag ccaggttcaa taactaccga
    17161 tatctcatca acgttataat ctccgcgacc aacaagagct cgcatggtag actccactat
    17221 aaatgccttg aagtctattt catctgtctt actcttcaaa aaacctagta ccgtaaactc
    17281 ctttcgaaaa ccgcctatac ttaccctaat tttgtcccca atctcaacat cttcgagcaa
    17341 tgatatattt ggagactcaa tattcaaata cttcttaagc aggcttgatc caaggactat
    17401 accatcaaca tcatcagagt taaggaaacg accctcaata ataaactttg aaatctgtgt
    17461 caccgtattc tcccggtctg gatcaagacc aacgatagtg gtcccagcag aattggcatc
    17521 atctgtctgg cgaattttct ctttgtactt tgcttctatc tcccctcctt ctgcgtatcg
    17581 ataggtgtag gctctcactc caggaaggct ttctagatta ctaatgatgc cttgactgtg
    17641 ctctataaaa ttcttcttcg gtaatttggt gataataata tcaccagcgt aattatcctt
    17701 ataaatattt gtagaacctt gtatcaaccc aaccaatacc cctctaacca ctacaaggtt
    17761 gaggaaagtc agagtcataa caaagacgat aagtgcggtt gttgcgaagc tagatcgtct
    17821 tacctgccta tacgccaaga accaacccaa ggagatggat ctaccgaaat gctctattct
    17881 ctccccaaag gtcttcattt attctattct ctcattttct ggcttaaagt cgagcctatt
    17941 taggtttaca gttcacagct agttcatatc cagcctcctc ggcttgggcc gctgagtcga
    18001 aaaatatttt gttcttctcc gagacagtgc tcttgcagtg tggatagtag tatctagtgc
    18061 cgttaactga cgcgctaatc ttacccgtcg taacagagct agagacatta gcagctgagg
    18121 cggtgttttg aagcaaactc tggtcaaaat tgatttcaac cccctctctt tgtgtggatg
    18181 taagcctccc aacaccaaag gaaagcaatg cgactaagac gatgacaata gccaaaaaat
    18241 agggctttat agggcttttt tccacaacct cacccctatt gtcaaaatgg aagacttcgg
    18301 gtatactcac tgagttaaat tatagtctat ggcaacaaaa aagtctggtg gaacaacgaa
    18361 aaacgggcga gatagtaatc caaaatacct aggtattaag gttaaccacg gcaccgctac
    18421 taaagtgggt cagatcctcg tgcgtcagag aggaactgac gtcctacctg gtaagaatgt
    18481 tggcataggt aaggatcaca ctctatttgc acttaaggat ggtaaggtca ctctcaccta
    18541 taagaggaag actcatttcg acaacacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnncga agtgagtttc tttagagccg aatttaactt ttatgctgtg ggtgcctaat
    18721 tccttaatac cattacctac ttggatattg tctgcactta tctctatccc cctctcggaa
    18781 agaagtttag aaatcttcgg tgctgtgagt gctgcaaaga ggctgttctt ctcgtttgcc
    18841 ttagctttca cttctatcaa agtgttggta agcttctgaa tctcgacctc tagcttcttc
    18901 tcatcagcct cacggtgctt ggcgtcactc tccttcatag tcttgatctg ccccgctgca
    18961 ctaccagtag caggtactgc aagcttctta ggtatgagga agttctcggc gtagccgtca
    19021 ctaacattct tctcctcata cctcttcccc acccccttaa tgtctttgag taagattact
    19081 ttcatatttt tcaatacctg gattctactt cccagtcttt aggaattcaa ctgcgcgctc
    19141 gagttgcttg tctctacctg tctttatgtc ttcctcagtt cttttaataa ctatctttgg
    19201 agttatacct tgctttgata ttgagttgcc atttggtgta agccagcgag caatagtaac
    19261 cttgatagag gtgtccttgg tgaccggcaa gagctcttgt actgacccct taccaaaggt
    19321 ggtgtcgcca atgagtgtcg ccacaccgtg ctcagagagt gctcctgcaa ggatctcaga
    19381 agcagatgca gagccagcgt caactagtat tgccatctta agactatcgt tgaagatgtt
    19441 gtaaccctta gagcggtaga cattctcttc cttaccatca cctctgtcct cgcgtataac
    19501 aggagtacca gatggcaaga accatgatgc catgtcgact gctgtctcta gatatccgcc
    19561 tggattacct cgtaggtcta agaggagctt gtcgctatct gactcgatga actcgcgcag
    19621 tgcggagcgg aagagtacag ccgagttctc ggtaaagcta tacaaagaaa ttctaaatat
    19681 tcctccatca agttcctcag tattaataac cgggacattg ataatctgtc taaccacctt
    19741 gatatcaaat ggcgcggtct tgccctgtct aaagacattg atagtaacag tagtgccctc
    19801 tggtcctctt atataagtca cagcttcttc tactgtgagc ttggtcacat ccttgtcatc
    19861 aaccttaagg atcttgtctc cgggtcgtaa cccagccgct tctgcaggag agcccttgag
    19921 tggtgagatg acggtgatga tcccgtcttc ctgaccaacc tccataccca caccactaaa
    19981 gctaccctta atatctgctg caaattgctt tgactccaca ggtgggaaga agactgtata
    20041 agggtcatca agcgagctaa ccatgccttc tattgcaccc caaaccttat cttgatctgt
    20101 agctactttg gtggatgtac ctgttgggac gaaatctttg ttaattatcg accatgcttt
    20161 ccaaaatggt gcgaagtcca cagtcgttgg ctgtccgtct tcgacattac tcagaccact
    20221 cttaacatta ctaatgagag tcacactctc tcctctcttg tatccagctt gaaaggcgat
    20281 caaggcgacc aatacgaaac ccactattgt ggtgcgtgac atctttccta aatccattca
    20341 gaccattata ctagcaataa tgatttcctt ccataattcg cttaccaaca aggtcgagct
    20401 cttcgtgcct ctcaaggatg gcgaggtcaa aatgtatcac tgtggcccca ctgtgtacga
    20461 cttcgtgcac attggtaacc tgcgctcatt cattttggct gacttaataa gaagatactt
    20521 tgaatacact ggctccaagg tgacccaagt aatgaacatc accgacatag gacacctctc
    20581 aggagatgga gacgatggtg aggacaagat gaccaaggca ctcaagcgcg agggaaaatc
    20641 tttgaccatg gattctatgc aagaactagc taacttctat actgagagat tcaaagaaga
    20701 tactctgacc ctaaacatcc ttccgcctca ccacctaccc aaagcctcaa atcatataca
    20761 agaagacata gagcttatta aagaactaga tgcaaaaggc ttcgtctaca aaactagtga
    20821 cggactctac ttcgaggtct cgaagttccc tgcatatgga gcactcggtg gtaccggtgc
    20881 cggtgagtcg aggattggag agaagagtga gaagagggat cctcgcgact tctctctttg
    20941 gaagtttaat actgagcttg gatacgaggc tccttggggt cacggcttcc ccggttggca
    21001 catagagtgc tctgctatga gtcaaaagta tttgggcaaa acttttgaca tccacaccgg
    21061 aggcgccgac ctcgcgccca tacaccataa caatgagata gctcagagcg aggctgcgtg
    21121 cggatgcgaa tttgcccact actggttaca caacgagttc gtaatactcg acgggtcgaa
    21181 gatggccaag agcgatggca atatcatcaa gcttaaggat attgtagaca aaggtttctc
    21241 cccactcgct taccgatact tcctccttct ctcccactac cgtactccta ccaacttctc
    21301 cttcgaggca cttgaggctg cacaaacggc gtacagaaga ttgaaggaag taatgagcgg
    21361 tgaagaagga aaggtaaacg ctgaatacaa aaataagttt ttaagtgctt tagagaatga
    21421 tctaaacaca tcgcaagcat tagcagtagt gtgggaccta gtcaaagatg agtcagttcc
    21481 gaaaggagat aggaactcaa cactcttaga cttcgacaaa gtacttgggt taaatttgaa
    21541 tcaaaacgag tttgaagtta agaaaattcc aagtgatata caggatttaa taaatgaacg
    21601 tgaggttgct agaaaaaata aagacttcgc aaagtcagac gaactaaggg atttgataaa
    21661 caaaaagggt ttcgttattg aggactctgg agacagctac aaaatttcta aagcttagtt
    21721 accgtattta gcgaggattt ctttctccac ctcttcccta ttcctaccgt actttagctt
    21781 ggttttctca gccaagatat ctattagctc tctattacct tctgttggac tcatggtctc
    21841 catggagaat ggcttctcgg gtctaccatc cacaagcaac ttcaagaatg catttctatt
    21901 atcaatattc ataagatctt tggcagtaaa gactggagca aactgtttct ctaagtactc
    21961 tgcatcgtct gctcctactc ggaaggcaat gatagatcca acgttgccga agactgcatt
    22021 cttaatactc tcctcaagtt gcgcaatgaa ttggtgcgcc acagtgagag aaagtttata
    22081 tttacgcgcc tcagagagga tggtggcgat agaatcagta gtaatgttct ggaactcgtc
    22141 tatatgaagg tagaagggtg gaaaaccagt agaacccgac ctagaaagtg ctgccataag
    22201 gatcttaccc acgagtatta gaccgatgag attcgcattc acatccccaa gtctcccctt
    22261 cgaaagattt accaaaagga tcttcctatt atccattatc tcgcggaagt ttattgagga
    22321 cttctcctga gcaatgatcg gacgcatgat atcgtttgct aggaagacat cgaacttcga
    22381 agtgatgtag ggtactatgt ttgcaagagc tgcctctcca ccggctttgt ccgctacttc
    22441 tctccagaac tgtagtacta ctgggttggt acacttctcg agcttcatag ttcgaaattc
    22501 tttgttcgag agcactctcg agacatcgag cagagtcgaa ccgctagacg gatcatcgat
    22561 aactagcatg gtagcgtttc taaaatactg ttcgaacatc ggtcccatac tctctggaac
    22621 cttgccgtag agtttctgga atatactgaa gagctcgttc accacgaagg tcttctgctc
    22681 tggcttgtcc ttgtcgtact caagcatgtt caaagccatt ggtctctcgg tagatgctgg
    22741 gtcaaagtag atcacatcct ccatcctttc gggtgggatg ctagctagta catcttgaac
    22801 atcctgaccg tgggggtcga tcatacacac accattccct gccttaatat cctggataat
    22861 aatattcttt agcaatgtcg acttacctgt acctgtctgg ccgatagtgt agatgtgtcg
    22921 taacctgtct tcgtaactaa tatgtacctg tctgtcgtgt ccttggaagt ggttcgtacc
    22981 aagcaccgtg ccaccaagtg acatcgagag tggtgcctga gcctctgagg tcttagaagt
    23041 ctttagttgg gcactagatg aaagtgaggt ggtgtggaag tggagaagtc ctgtgagctc
    23101 cttcaagtta agaggcatga tctctgcttc attccacaat cggaaggtga agttgcggaa
    23161 gaacttttta cccctagcct cgcgccagtt aagtctgttc acattgggta tttcaaattg
    23221 attgaaggag gaggcgatac tagacacaat gctcttggcc tccttttcgt tctgggcgga
    23281 cactgcaatg cggatacttg ttgccacaat agggctcagt aactttttat caatattctt
    23341 gagtgcctcc gcatctactt tggttggatc aatcttctta ccaccaaaga actccgacaa
    23401 cccatgcacc acctccccca atatggacct ctcagccaaa acatcactcg acttatgacc
    23461 cttctctata cgtttcttgg cgaaagtgta cttcttggta taagcgtcac cctgtgggtt
    23521 aaatattatt tggattgctg cgcccgagcc gtctctaggg attttggaga aggattgtaa
    23581 gagtgagcta agtgggtcat ggtcgaattc gtcataagtc tttagaggaa aagcacttga
    23641 ctggttaagc tctgccactg ctccggttgt gaaggcgtgt tcggagaaaa tgttgtaatc
    23701 cctacttctt tcaacaagcc ttacattagg aaaaacggat cgtaagtgct tctcgaacaa
    23761 atctctcttc tcattcggta cggagacgaa gaaatttgcc tcctcactcc cctcgctgtt
    23821 agctacttct aacacaaaat agtttttacc acgagttgct tcctccgaca ccgagatcat
    23881 gccggcatag aactgctcca tggacgaaat aaccgacttt aagtcccggt cctttgtccc
    23941 atcgctccca atgtctggca tctgtatctc aaagagagtc atattaagag cccgatacat
    24001 tggaccactc tctttgacat ctttaggttt gaatccccca cgtaccagct ctaccagata
    24061 gtcatggaag tcgtcctcat gcttccagtc ctcaagcttc tctgccacag agagagcatg
    24121 cataaccccc tttgtacgac ttatatccat catgtggtcg agctgagagt ctgttggctt
    24181 gtttgagaga gtctccaagc tttgcttaac atctgtagac tgtctaacct gcacaggcaa
    24241 ctcctggtgg gtgtttatga tagtacgctt aacctctgta gaattatcct tatacgactc
    24301 aaccttcgag cgaaggtaag aaagctcttc ctctacacta ttaaaacgtg gtgcctcaga
    24361 catgaaaaaa ttatatcacg ctaaatagat gcctctcttt ccatctctaa tttccaatgc
    24421 tttagaaagt ccattatccc ctcatgcaca agatctttgc ttttaatctt gactgcctct
    24481 ttggcaatgt ctctctttcc ttccaaaata tctctaacaa tttgcaatct gtctctgtat
    24541 aatcttgcaa tctcatcttg accatttagt tccgcgaatc ttgcgaagac ttccatctgt
    24601 tcctccatct gattaagaat cattatctgt attctgtgtg tagcaagctc tttgtttctt
    24661 ttgaactgcc ataattctgc tagctcaagc actgtttgtt tgcttgctcc aattacaact
    24721 tctggaatca tactaagctt gccgtggaag taaccgtcac ttgaattgaa gtactttacc
    24781 tgcggcaacc tacctttaag tattccttcc ttcaatcgat cgaactttct tgatgtatca
    24841 gcactaaagg atacatccgc tgccaaaccc aagaaggaag cggctccggc catttcactt
    24901 tggaattcta tgatcgcgtc caccccattc tcgtaatcat catatttaga ggtcttgtga
    24961 gtaatagcgt tcggaccaaa ccagtcattc aactctccat tctcaagcac taaggcctca
    25021 aagatatctg caacagcctt ctgctcctta gtatactcgt ttgtgttgtg atcaaaatcg
    25081 cgctcgtgtc ttctaacggt ctctagatct ctatcaatgg tctcttggcc gtatgggttc
    25141 acaaaatcct tttccttaat cctggcttta tccataactt tctcagccct accatgtatt
    25201 accgatatgt cagctctttc taatccctct ggtacaaatt tttccatatt cattgtgtaa
    25261 ttaattatac acaccttacg caccttctct ccaccttttg cctcttggag cactgagtct
    25321 atggagcgta taatacgcac atgcaagagt cctcactagc gagtctaaag atccgtgtcc
    25381 ctccacacaa cctagaagct gagatggctc tccttggttc tgtcatgctt cgaccagacg
    25441 ctatatacga gatcatggac atctcgagcc ctgcgtcctt ctacttcgag aagcaccgca
    25501 ccatcttcga ggctatgctt gaactcttcg ccaagcgaga acctatagac atcctctcct
    25561 tgtcttcgaa gcttaaggag aaggggttac ttgaaagagt cggaggagcc tccttcctta
    25621 ccgagctcac caccaccgtc ccctcctctg cgaacatcaa gcactatgct gaaattgtga
    25681 gaaagaagtt catgatgcgc gagcttatcg acgcttctga atacatctca cacctcggct
    25741 tcaatgagtc gggagagtta gagaatattc ttgatcaagc tgagaagaag atcttcgaca
    25801 tcacacagca cttctcctct actgagaact tcaaggacct tactccccta cttcaagagg
    25861 cgtttgaaag gttcgacaaa ctttcttctg ccgagcacga gctccgcgga gtaccgactg
    25921 gcttcaagtc gctagatgac attcttggtg gattccagaa gtcagacctc atcatcctcg
    25981 ccgctcgccc atctgtgggt aagactgcgt tcgccttgga tgttgctaga aggacagcca
    26041 gcttgcacca aacacctatt ggtatcttct ctctcgaaat gagtgccgac caactcgttg
    26101 accgtattgt gtctgcacag tctaaagttg atgcctcggt catcagaaaa ggagtgcgta
    26161 ctcagcaagg ctcttggcgc gacgacatat tcaaaaacat ttctactgca ctagacgatc
    26221 tctctcgcac ccctatatac atcgatgatc agtcaggtaa caccattatg aagatgcgct
    26281 ccgctgcgag gaaattaaag atcgagaagg gactccaact cctagtagtc gactacctcc
    26341 aactcatggc accgacgaac gctcgtgccg gtgacaacat ggtccagcag attaccgaga
    26401 tctcgagagg cttgaagcac atggctcgcg agctacagat cccagtcctc gccctctctc
    26461 agctctcgcg tgcggttgag actcgtggcg gcaagcccaa gctctctgac cttcgtgact
    26521 caggttctat cgagcaagac gccgacctcg tgatgttcat ccatgcagac gaagacggta
    26581 tccgagacga atccggcaag ataaaagagg tacagagaaa aagcttgatc attgcgaagc
    26641 accgaaacgg ccctctagga gaagttgtcc tcgactacca tactcggaac aacaccttcg
    26701 ttgagatcga ctacagtaac tacggcaaag ccgaagaaga gtttaagagt ttctaggtaa
    26761 ataattttct ctgactccaa agatatacag atgaaatgat acaaattatc attagagcaa
    26821 gaaaaaccaa gattaagtga ggtagggata aagtcataat aacaagaact gagagcacta
    26881 ttgggagtat tactagcagc atgttaaggg attttctttt taacattttt actccataac
    26941 tgacacgggg caaacttata cctaaaagga aggtcgtcaa aggcattatt atgaaggctt
    27001 ttggataagg gccactccca acgttcataa ttagtgccac aataaaacct agtactgaaa
    27061 aacacaataa cgctacagcg agtaaattaa ttaatgtgct aagacgcttt atatttttta
    27121 tttttgaatc attttcattt tccatccatt tattctaact caattttaag ctaaacttgt
    27181 ccaatgaatt cgatagacac tcttgccgag atatttgccc actttcctgg tattggtcct
    27241 aggcaggcaa agaggttcgt ttattacctc ctctctcggg acaaaagcac agtcgattcc
    27301 ttcgttagag cggtggagaa gttgaagcaa gagaccctcc aatgcacaga gtgcaaaagg
    27361 tattttgcca ggaacggctc tgacaaaccc atttgtagtg tttgtagtga ttcccaccga
    27421 gataagtcac tcttgatgat agtccctcgc gacgccgact tcgacgcggt ggagaagtct
    27481 ggaagttaca aaggatacta cttcatcctt ggtgggtcag taccaatact tgagaaggaa
    27541 ccagagaaaa ggataagact gagtgaacta tcaacgaaga taaatgggaa taaaagtgtt
    27601 ttgaaagaag taatactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag
    27721 tcgatgcgga cctcaaagta tggttggcgg tgaccgtatt tcttgaagta cttggacttc
    27781 tgcttgtagt ggatcacgtc tacagtcttg tatcgactaa tatcaatgat agttgagtgg
    27841 actgaggcag tgccaatggt tggattacct agagtcatct tgtcaccctc attcttcata
    27901 agaacctctt tgaatacgag cgcatcccct accttgtggt cgccaaggat cttctcaatc
    27961 ttgagcattt gaccctcgtg gacacgatac tgcttgcctc ctgtcataat gacagcaaag
    28021 ttggtcttag acactgccat aggccttcta gacgcctttt gagcggtctt tggagcctcc
    28081 ttagcacctt ctaccttggc ctttggggcc ttggcttttg gggctgattt cttagctgtt
    28141 ttagcttctg ccataggttg cctattctac attaggacgg tctattgtca acgtatctat
    28201 cccactagat gagcctgtaa tacggagcac atccttgtct atcttaccca gctccttttg
    28261 gcctgtattg tagtggttta caacggtgcc caatgaagtg cctagcttct tatagtattc
    28321 gtcataggtc ttgatgtggc gacccaagtc ctccaccttc ttgatgatct cttgtgcctt
    28381 ctcctctatc tggagagcct tcaagccttg cattactgtc tggaggtacg cgaggaagga
    28441 ggttggggag acgatgatca ccttgtactt cgaagcagct ctctgtatca agctttctgt
    28501 ctcttcttgg agtgctccga tcttgttcac aataagatca tagtagatag cctcgtgtgg
    28561 gataaacatg aaagcgaagt ccatagtccc cttcgacggt tggatatact tagcggtctc
    28621 ttgtatacgc atcttgaggt cgcttacaaa gagcttttcc aatttcgcct tctccactgg
    28681 gtccttctcc tccactaacc tgttgtaatt ctcgagagag aacttggagt caactgggat
    28741 aatcttctcc ttcacaaaca cgactgcatc aggcactagt cttgctccat catccgtaat
    28801 acccaactca tactgcattt cataactccc aggaggcaaa acatttttga gtaatgtctc
    28861 aagatagtac tcaccgagga tccctctttg ctttgggttc ttcaaaatgt cttggagact
    28921 ctcgagctgg tcagcgaagg agaccacttg cctgttcgtc tcgtctagtt tggtcaaacc
    28981 ctctgtcacc tcgcgaataa gctttgctga ctcgccgaac tgagtcttca ccgactcgtg
    29041 cattagccta ttaccctcgg tcatcttctg gtccacagtg cgtgagagct cattaagctg
    29101 ttggagcagg agattcatcc cctcgctctt ttctcccccc tccttcttct tcacaaatac
    29161 gaagaaaatg cccaagttga gtgcaaacaa agcgaccaag aggacaatta tccaattcat
    29221 tggtatcatc ataacatgtc gctacaaaca gatataaaat caggtatcaa ggatgccatg
    29281 atcgctaaag acgcggtccg tttatcagtt ctaagaggtt tatcagcagc ctttaccaac
    29341 gagttagttg ccaagaagat gaagcccgat ggcgaactct ctgacgagga cgcactcgca
    29401 gttattagta aggaagcaag gaagaggaag gattctatag agcagtttga gaagggcggt
    29461 cgaccagagc tagccgagga cgagaaggcc gaactcgcgg tactctcaac attcctccca
    29521 gagcaaatga gctatgaagc agtgctcgag ttcgtcaaag ctaaacaagt agaaatgggc
    29581 gcaaccgaca aggctaaggc aggtcagttc atgggctctg tgatgaagga tcttaaaggc
    29641 aaggcggacg gccaggtggt caaggcagct atcgactccc tatttaccta gccctctcca
    29701 cagagacctc ccctccgtag aagttatcca cagtttttag acaagccctc cccttggagg
    29761 gttttgttta gcttgacttt tgggcttgtt tgtgatagta tttaaataga aaagatccat
    29821 acaactcaat ctattcgtcc ttccgggagg acaaatggca accaagacag aagagaaggt
    29881 cgaagagacg ttcagcaagg aattgctgtt tgacttctct gaggccttca agagaaacaa
    29941 ggagttgagc cccagacgga gtttctacct cgtcttcctc gatacctgtc gagagttccg
    30001 tatcttcgga gagaggcaaa aaaagttctt cgaagtcttc gccatctata gggcgacagt
    30061 tcgaattcct gaaggcatca gggagagtgc gtatttcctc catgaggagg ctccacacgt
    30121 tgtgatggcg ccagttgtgg tgcccgtcac tccgccagtc aagaaggtga gatcgctccc
    30181 aaacatatct gttaggagtg gcgatactca cgacagacac gagagccagc tcccgcgtgg
    30241 agacaggaac gatgatgatt gagttacaaa gggcgga
//
LOCUS       DAORIT010000003        24094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_336_length_24094_cov_54.820583, whole genome shotgun sequence.
ACCESSION   DAORIT010000003 DAORIT010000000
VERSION     DAORIT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24094
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_336_length_24094_cov_54.820583"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLD99_00520"
     CDS             complement(<1..431)
                     /locus_tag="PLD99_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25423.1"
                     /translation="MPGSQNIILWMVAQNLPLGHFEKYLEGGSLAKDASSAKSYTMKK
                     KETIARAIVNGLSQYAVAGQVAKEWDSFDPQIRMLITDMCNNTASLSGNVRKGVARLD
                     QLTGGKIRRNGTGPTERKLIVVDGTVVSVRQRVNTAMTNAER"
     gene            533..1039
                     /locus_tag="PLD99_00525"
     CDS             533..1039
                     /locus_tag="PLD99_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25424.1"
                     /translation="MKQGPESVPELRLVELGVTKSGEKVFDRPSSHIHGNETVEKYLP
                     LALERIDSMGRDFIVEAVDFGEEIGVSMCVETTPEDEIVYAVRPKRRGPTRFVKNREG
                     EPTSEFTVILKKVDEGYIVITAFVGPKAEKEPWDPMATEESKEFWNNHALIWESEEVI
                     PGTESTTP"
     gene            1122..>1569
                     /locus_tag="PLD99_00530"
     CDS             1122..>1569
                     /locus_tag="PLD99_00530"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRH25425.1"
                     /translation="MTTVIILSIVGAVIAAVVGTFWYSNKTPMGRIHMQYLGFDKLSD
                     EEKRQKMEEAKPKMTKIYGAQMALSLLTAFAVSYITVIGMGRGGLTIWMVLGFVLFNW
                     ICFIVPTIGTHILWGNCDPKIAWKKFFSDSFSVLTTLIITTLLASLF"
     assembly_gap    1570..1669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1670..2093
                     /locus_tag="PLD99_00535"
     CDS             <1670..2093
                     /locus_tag="PLD99_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25426.1"
                     /translation="MKKLILVLVVLSIGTLCFFQFKKEPYTLIQDYRKTNSDEFKYFG
                     NEVDQDLNNDGVVDTAFIATQEVSGQTSFYLIGAIKKDGGYLLTKSFLIGENISPQTT
                     ERMEGGVVINFAIGSNPSIGKSLRLELNSKTLEFVEKL"
     gene            2186..2392
                     /locus_tag="PLD99_00540"
     CDS             2186..2392
                     /locus_tag="PLD99_00540"
                     /inference="COORDINATES: protein motif:HMM:NF018096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HRH25427.1"
                     /translation="MKTIKCSEVGGDSCDFSVTTSTIQEARMKLLEHAKEAHPEMFKI
                     ATPDARTEWETKFKNVWDHAAEIV"
     gene            2437..2622
                     /locus_tag="PLD99_00545"
     CDS             2437..2622
                     /locus_tag="PLD99_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25428.1"
                     /translation="MENLDRIIVNNGGGLGSLWTIGWLFTIGYLKLSFWKGVLALFIW
                     PYFIGVHYSKHHQNKAV"
     gene            2625..2963
                     /locus_tag="PLD99_00550"
     CDS             2625..2963
                     /locus_tag="PLD99_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25429.1"
                     /translation="MNFLQTSLIVLAASSVAVADIFLKKTEGLGSINKALSSPWMFGA
                     LALYLFQIIFFTYAFVSGSKLVNVGVMQVALYSIIILLASFFIFNETLSQIQVLGLLL
                     ALSGIIFMHM"
     gene            2992..3495
                     /locus_tag="PLD99_00555"
     CDS             2992..3495
                     /locus_tag="PLD99_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097159 - organic cyclic compound binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5-like heme/steroid binding
                     domain-containing protein"
                     /protein_id="HRH25430.1"
                     /translation="MHNKFIPVIIGLVVAASTGIFVQSTKNPSIPNQVPTSDEVTANY
                     SSSKTLYNYDEDEDEDDDDGVSGSTVSTPATEATSATPQGTTPSTGGITLAQISSHSS
                     RSSCWSAINGVVYDLTSWIPNHPGGEGTILGLCGRDGTQSYNGQHGGKTKIFNILTGF
                     KIGTLAQ"
     gene            3498..3905
                     /locus_tag="PLD99_00560"
     CDS             3498..3905
                     /locus_tag="PLD99_00560"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRH25431.1"
                     /translation="MNYYKQLTRQEWGLLLLRFGLAIVFLWFGFSQLLDGLKWVSIVP
                     AWATDLFNIPPAMIVLANGLFEVVLGTLLAMGFWVRIVSFVLALHLIPITVDMGNTAT
                     GVRDFGLVVSAIALSLLYSKKNTSIAIENKNQY"
     gene            3923..4690
                     /locus_tag="PLD99_00565"
     CDS             3923..4690
                     /locus_tag="PLD99_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRH25432.1"
                     /translation="MEKKYIYGLVGLGVIVVLFFLIDNLAYESGRQGIIVGETVSRSG
                     KIVSIDTNQIAFDGPYIFSLESEAGELTTVAIPSMGLPMCEAYKAKKIGDVSLIKISD
                     EMEVRGVVSEDGSIVPCSSGEHYFTPKGIVVENFEGEADPNRMKLTMKTWNWVNTLLN
                     DGTTITPKIENKFSISFKEDGTFSAATDCNGVGGNYVTKDSYIVFSQMMSTLMYCEDS
                     QESKFTEFLANTSNFHFTSKGELVLDLKFDSGSVIFR"
     gene            complement(4677..5942)
                     /locus_tag="PLD99_00570"
     CDS             complement(4677..5942)
                     /locus_tag="PLD99_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRH25433.1"
                     /translation="MDSIYNKEWDVIVVGGGPAGMMAAGRAAERGAKVLLVEKNDSLG
                     KKLLITGGGRCNVTNAEPDVRKLLAKFKESEQFLFSAFSVWGVKETLDFFNGKGMETK
                     VEAEQRVFPLSNKAHSVWGVMVEYIKKHGVTVLSDSPVQELLLDRGIITGVILKNKKE
                     IRGKSVILATGGTSHPETGSTGDGFKWLTDTGHKVVKPSASLVPIAVKDDWVARVAGL
                     TLPKVKIKLLQNSVKQEESKGKILFTHVGLSGPTILNMSSSVGELLKYGEVVISLDIL
                     PDLDHSMLNSKLQEIFKLEDKKKFKNSLKSLVPTSLAPIVVSLSKIDPDKKCNSVTRE
                     ERLSLINLLKNLDLSVLGLLGEDKAITTSGGIDLTQVDFKTMQSRLFSNLYVVGDVLN
                     IDRPSGGYSLQLCWTTGFVAGNSVSIIER"
     gene            6012..7511
                     /locus_tag="PLD99_00575"
     CDS             6012..7511
                     /locus_tag="PLD99_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRH25434.1"
                     /translation="MAKDGVILRFEDLSFEYGQNKPILEEVSFTVREGSKITVMGQNG
                     AGKSTIFGLITGSIKPDVGHVFLTQGLTVAISRQTIPREELTLTVREFFERAFQKSSE
                     ANLRKDDGKIYDIDKRIDEVLEVVNLKSKSDVHTRIMNSFSGGQQARLLLASALIQDP
                     DLLLLDEPTNNLDKAGIEHLTQFLKDYKKTVIVISHDADFLNSFSDGVLYLDIFTKKV
                     EQFVGNYFDVVKQIAAQVEKENRKNAQLEKEIQANKDKSNFFANKGGQMRLVARRMRD
                     KAAELEESIIDTRKEDKTIRTFVIPVQDGVSGDLLTISEFTVLNLKTHKPVKRKANIK
                     LRKKQHLLLKGPNGIGKSTLLEAIAKGEMAGTNITPGVKVGYYRQDFSTLNFEDTVYD
                     SLMSVMEKKNEETMRATAAGFLIHADSIKSKIGSLSEGQKGLVAFARLVLEKPGVLIL
                     DEPTNHINFRHLPVIAEALDKYDGAMIMVSHVPDFVEKIRIDEVLDLEK"
     gene            7551..9758
                     /locus_tag="PLD99_00580"
     CDS             7551..9758
                     /locus_tag="PLD99_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRH25435.1"
                     /translation="MYLKSIELAGFKSFAKKSTLEFSSAISAIVGPNGSGKSNVAEAF
                     RFVLGEQSIKSLRGKKGEDLIFNGAQGEPKANRASVKVVFDNQKKLFGLDFSEISIER
                     VVHRDGVNEYLINGSTVRLKDVIELLAKAHIGASGHHIISQGEADRILSATDRERKSM
                     IEDALGLKIYQYKRLESDRKLKKTFENIAQVEGLRKEIAPHLRFLKKQAEKIEKVEGL
                     KNELLQLSLEFFKKEEEYLNHEKNLIKSEREPIEEALKTLHNELAEAKKVLEESAQGK
                     DKNLEILELESEIARVRSSKDALVRELGRLEGLMNAEERAIKKEEELALNEESKTVRL
                     KEVEELFEEVSLLSTIESVFLKIKEFIKSRKQASGTDAIVEAREQLSRYKDKKLQTES
                     EFALLEAEETKLRDKYESLKRKIEQDKDSSRDAEKAVFRIMSEENEILSRKNILKIRE
                     ERLHHDDEAFKRELAELGHMVGAEILAFRQKDYAMEADRNLQEERKRALERLKIRLEE
                     SGVSGAGDVLKEFRDVTERDEFLARELLDLEKSAESLKELIKDLEKRLAEEFSQGVAK
                     INKEFRELFSTMFDGGDASLTLIKEKKKKIVPSDTDLDLGVDVSDTEEEEVEEGLEIA
                     VSLPRKKIRGLMMLSGGERALTSIALLFAISLVNPPPFIILDETDAALDEANSKRYGD
                     MVEKLSKHSELILITHNRETMSRAGVIYGVTMGSSGVSKLLSIAFEEAVEVAK"
     gene            complement(10083..10403)
                     /locus_tag="PLD99_00585"
     CDS             complement(10083..10403)
                     /locus_tag="PLD99_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25436.1"
                     /translation="MPRYMVVTIGGAEEIMIQPINEFNTGWQWDGIKVSPPDVIPARW
                     SISKKPESVGIKVTDYCQLGVFIRSQIGAAHFITIFDLDDHVQHRRAMINLIKLHTPG
                     GQQI"
     gene            complement(10463..12373)
                     /gene="rnr"
                     /locus_tag="PLD99_00590"
     CDS             complement(10463..12373)
                     /gene="rnr"
                     /locus_tag="PLD99_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRH25437.1"
                     /translation="MKDELVGVIKVTGKGIGYFALPDTEVDLEVQSESINTALNGDTV
                     RVELLDKELYDRKQAKVVEIVKRKKMHFVGTFEDDNGKCFVIPDDKRMYRDLFIDTGN
                     SMGAKQGDKVVSELVEWPEPSKNPIGKVIKIIGRAGEHNAEMVGIVYESGFEVDFPTE
                     VEKEAREWKERWTKEDHLKDRKDFRDTTTFTIDPADAKDFDDAISIKKINNGDYEIGV
                     HIADVSHFVVPKTALDDEAVKRGTSIYLVDRTIPMLPEILSNDLCSLNPNEDKYAFGA
                     VFVMNTSGEVKERWFGKTLINSDKRFTYEEAQDILDKKEGMFYEELNTLNQIAYKLRE
                     EKFKNGAIEFESDEVKFELDSTGRPVKVFRKVRKDTHKLVEDYMLLANREVARYMHTM
                     TNGNTKAAVVYRIHDAPDREKIINLATFVKALGFNLKHEGGETTAQEITKMLRSVEGT
                     PAEALIKTASIRSMSKAIYSTSNIGHFGLAFEYYTHFTSPIRRYPDLMVHRLLQRYLT
                     QGKIEADEVARYQKLSDSSSERELEAAEAERSSIKYKQVEYMSDKVGQEFDAIVSGVS
                     EWGVYVEETTAKADGMVRLKEMTDDFYELNEKQYAVVGQKTGKKYSLGDKVRVKLLDA
                     NLDKKILDFKFV"
     gene            12434..12859
                     /gene="rpsP"
                     /locus_tag="PLD99_00595"
     CDS             12434..12859
                     /gene="rpsP"
                     /locus_tag="PLD99_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRH25438.1"
                     /translation="MLKMRLQRVGRKHDNAFRVVLTDSKNSSKSGKFKEVLGSYDPRK
                     TVDVLKTDRIKYWLGQGALPTGTVHNLFVTHKIIDAKKKNVLPKKTPIVKEAPVVEEK
                     PVVASAPAPEVVVEAPVEVAPEVAPEASEPVVESTEVAE"
     gene            12899..13231
                     /locus_tag="PLD99_00600"
     CDS             12899..13231
                     /locus_tag="PLD99_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRH25439.1"
                     /translation="MLDDVQFLEFLIKALVDHPEDVKIKRTVDEMGVLMTLDVNPDDM
                     GKVIGRQGNTAKAIRILLRVVGMKNNARVNLKINEPAGGRTFAPREEYTPRASKTIDE
                     AMEDLNLE"
     gene            13237..13887
                     /gene="trmD"
                     /locus_tag="PLD99_00605"
     CDS             13237..13887
                     /gene="trmD"
                     /locus_tag="PLD99_00605"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRH25440.1"
                     /translation="MHFHIITLFPGTFDSYFGESILKRAIEDKKLKVSFYNPRDYAED
                     KWARVDRPPYAGGPGMVIQAEPVIKAVEAAIKKIKSKKYKIIFLSPSGKQFTNEYAKK
                     TAKTYKDIIIISGRYEGIDARVKKVFKMEDVSVGPFVLTGGELPAMLMVDVISRQVPG
                     ILGNFDSLEESRTASPDVYTRPESFTYKGKKYSVPKVLLSGNHAKIEEWRRKKKGV"
     gene            complement(14254..14799)
                     /locus_tag="PLD99_00610"
     CDS             complement(14254..14799)
                     /locus_tag="PLD99_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HRH25441.1"
                     /translation="MKRRALVSVTDKRELDKLKVLTDNGWEIVSTGGTAKKLEELGIP
                     FTPIEAVTGFPEMMDGRLKTLHPNIFGGILADRSKPEHMTAIKERGIVPIDLVVVNLY
                     DFESKPSIEQIDIGGPSLLRAAAKNFESVVVLIDPDDYEWTFKILTTGEVSYDSRRSF
                     AWRVFQEVSAYDHSISRWFIH"
     gene            15071..18259
                     /locus_tag="PLD99_00615"
     CDS             15071..18259
                     /locus_tag="PLD99_00615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRH25442.1"
                     /translation="MSTKTSARPQKFFGRFREPLTEMPNLVVSQVESFKWLIEKGLKE
                     VFEEFSSIKDFSERKFQLDFVGFELDKPKIDEYEAKEKKLSYEAPLRVRIKLKNFIQK
                     TEKEQEIFMADFPLMTDHGTFIISGIERVVVPQLARSFGVFFTAQDVKGKKTFGAKVI
                     PGRGAWIEFETDADGVINVRIDRKRKFPVTVLFRAFGATTNEKILHLFDGQPEAKAYI
                     EKTLAKDHTITQDDAAIEIHKRLRDGDLASAENAREFVKSIFDAERYDLSRVGRFRFN
                     NRFDLKQSDKEMDKRTLSIEDLRIIIAHIITLNTTPNALPDDIDHLGSRRVRYVGELF
                     QQKIRVGMAQIKRNIQNRMSTIDTDVTLPVQFISPKPLQARMKEFFTTNQLSQFMQQE
                     NALTEIEHLRTLSALGPGGLNRARAGYEVRDVHPSHYGRLCPIHTPEGPNIGLILRLS
                     SYAKVNDFGMIETPYAVVKDGKITSEIKYLNALEEEQYNIAHAATPVEDNKIVNEVVD
                     VRYKGEPHSVKRSQVDYIDVATNQAFSIATSMIPFLNHDDANRSLMGSNMQKQATPCI
                     IPEAPLVATGIEEKAVRDTGRLVIAPVAGTVTYVDGKLIILTDNKGDTHEFKLVNFSM
                     TNGFTSFHQRPSVEVGQKVKKGDVLADTSSSEHGQMALGQNILVAFMSWSGANYEDAI
                     ILSERLVKESKFTSVHIEDFIVNVRDTKLGPEITTCDIPNVGESKLKNLAEDGIIRVG
                     AEVRSGDILVGKITPKGESQLTPEERLLRSLFGEKARDVKDTSKRMEGGKRGRIISVQ
                     VFSRERGDKLDSGILKQIHIQVAQLRNVSVGDKLAGRHGNKGVISRILPVEDMPYMED
                     GTPVDMILTPLGVPSRMNLGQILELHLGLAANTLNYQAIVPPFVGAEEKEIKEELKKA
                     GFKENGKMKLFDGRSGEAFDQEIAVGYMYILKLHHMVEDKIHMRSIGPYSLITQQPLG
                     GKAQNGGQRFGEMEVWALLGHGVGHTLREMLTIKSDDIQGRSAAFDAIVKGERIQEPG
                     TPASFNVLVNNMRGLALDVDVLKSRQDEPTKE"
     gene            18265..21900
                     /gene="rpoC"
                     /locus_tag="PLD99_00620"
     CDS             18265..21900
                     /gene="rpoC"
                     /locus_tag="PLD99_00620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRH25443.1"
                     /translation="MNNNRKTIDTKDFDAIGIRLASPERIKEWSYGEVTKPETINYRT
                     QRSERNGLFDEKIFGPDKDFECYCGKYRGIRYKGIVCEKCGVEITRSIVRRERMGHIE
                     LASPVAHIWFLKNVPSRISLIMGIPVSDLEKVIYFAGYIVTSVSESEKNRVLKDLDSE
                     FKSKVKSLHDDKAKEALKELAQQAKNDIESLVPGRVLDEVEYHRFGMKYSTIFEAGIG
                     AEAIFSILKSLDLKKLHAEIDEMITRAPATELARLHKRASTVQWMITSNIRPEWMFLT
                     RIPVIPPAIRPMVPLDGGRYATSDVNDLYRRVINRNNRLKKLKEINAPDVILRNEKRI
                     LQEAVDALIDNTIRHGSAGGSQAQRRPLKSLSDNLKGKRGLFRQNLLGKRVDYSGRSV
                     IVVGPELKLHQCGLPKHMALELFKPFVISELLKKELAYNIPGARRLIEDAIPEVWAIL
                     EDIIKNKYVLLNRAPTLHRLGIQAFQPILIEGNAIQLHPLVCTAFNADFDGDQMAVHV
                     PLSIEAQAEARLIMAADRNLLKPGNGEPVVTNKMLDIVLGTYWMTRIIAGEKGEGSYY
                     QSPNAAITAHDFGAVEFRSKIKVLATDSPKYKHFEGKLFETSVGRLLFNSILPTDYPF
                     INNEMDKKRMGELVDDLIERYGIENLPSIADKIKSFGFKYATKSGVTWGLDDVKIPEE
                     KPEIVEKGRKEVNKVIEQWNDGLLSENERIQKSVEIWQATKQEIESLIPRTLALKDSV
                     YDMTYSGARGSFGNLNQMAGMKGIIANVSGESLEFPITSCYKEGLTPLEYFITTHGSR
                     KGLTDTALNTAKAGYLTRRLFDVAQDEIVTEDDCGTKEGIVITKESASGIQIPLSKSV
                     KGRFLAKDVLDATGQTLFKTGHFVTRADADQIDKANLASVEVRSPMMCKSEHGLCVKC
                     YGADLGNNKIIELGEAVGTVAAQAIGEPGTQLTMRTFHAGGVAAAGGDITAGLPRVEE
                     IFEKRKPKSPAVISHVDGTVTEIKVSGHDRIIVVLPEIGETKTKGKKVDTEYTVPSAR
                     TIFVKVGNSVKKGEMLTDGSADLDELFKYAGRDITSKYIIQEVSKLYELQGAAVSHKH
                     IELIIRQMFARRRVKDPGTTTLTRGDIVEISEVNNTDVKSDSVIMGISEVSLSRSSFL
                     SAASFQHTPRVLIQAAIRGANDGLLGLKENVIIGRLIPAGTGFNGGYKQKLIWEETSK
                     QLKNGLI"
     gene            21858..23441
                     /gene="dnaG"
                     /locus_tag="PLD99_00625"
     CDS             21858..23441
                     /gene="dnaG"
                     /locus_tag="PLD99_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRH25444.1"
                     /translation="MGGNVEAIKERLDIAEVIGGYIKLEKAGANYKAKCPFHNEKTAS
                     FHISTSRQGYYCFGCGAKGDIFTFVQEMEGTDFRGALKTLAERAGIELKQERREAKGE
                     TDELYQALEVATEFFEKNLAKNKEALEYLKGRGVSAESIKTFRLGLANDAWRELREHA
                     NSLGIKDDVLLKSGLIKRSEEGRSSEPYDTFRGRIIFPLSDARGKVIAFSGRALAKDL
                     EPKYLNSPDTPLFNKSEVLYGFDKAREEIRKKNYTVLVEGQLDLVLSHQAGVKNTVAS
                     SGTAFTQMHLEKLKKISPRIILAFDNDDAGSKASYRSTALALSLGMEVKIAEIKGGKD
                     PADMVKEDEESWKEVLRNSKSAVESFISALVSRESSLAKVGKEVENKILPLIASMTSR
                     FDKEDAVSKVASLIGKSPANVGEMVMKIKPEVVHSLIKTEVESVVEEKEVMSRVEELE
                     NEIKTIEELQIGLDKGTDEYKRSELSKKELNSHLKISKLEGERNELKLRVARGGGEEL
                     LHEIVELGKKIDEERRNLL"
     gene            23457..>24094
                     /locus_tag="PLD99_00630"
     CDS             23457..>24094
                     /locus_tag="PLD99_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HRH25445.1"
                     /translation="MPKAKKQKKTKAKKISVKPRVKKVKAKKVSKPKVKKAKKVVVKK
                     KVLSKKNQEMETKMNRLIARGKERGFITYDEILKEFPNIEINIIFLDELYEKFQQAGV
                     DVLEGGGLLEISNPDDDKKFYSKSDSAYDSIQMYLKEIGRYPLISGNMEKELAKRIEE
                     GDEEARNLLAQANLRLVVSIAKKYANRSPDLTLLDLIQEGNLGLYKAVEKFDR"
BASE COUNT         7027 a         4933 c         5805 g         6229 t
ORIGIN      
        1 cgctcggcat tcgtcatggc ggtgttcacc cgctgacgca ccgagacgac ggtgccatca
       61 acgacgatga gcttgcgctc ggtcggacca gttccgttgc ggcggatctt cccaccggtc
      121 agctgatcga gcctcgcaac acccttccgg acattcccag acagggaagc cgtgttgttg
      181 cacatgtcgg tgatcagcat acggatctgg ggatcgaagg aatcccattc cttggccacc
      241 tgcccggcca ccgcgtactg cgagaggccg ttcacgatcg ctcgtgcgat ggtctccttc
      301 ttcttcatgg tgtacgactt ggcactggaa gcatccttag ccaagctacc accctcgagg
      361 tacttctcga agtgcccgag agggaggttc tgagcaacca tccagaggat gatgttctgc
      421 gatccgggca tgcttgtctc cggctgagtt atttgatgta cttctctcta cattctaata
      481 tagcatattt aaaccaaaag tcaatagtgt agttatttgc tataatacct agatgaaaca
      541 aggtccagag tcagtaccag aattgaggtt agtagagctt ggagtaacta agtctggaga
      601 aaaagttttt gacagaccaa gtagtcacat acatggcaat gagaccgttg agaagtattt
      661 acccctcgca ttagagcgta tagactcgat gggtagagat tttattgttg aggcggtcga
      721 ttttggcgag gagattggag tatctatgtg tgtagagacg actcccgagg atgagatagt
      781 ttatgcagta agacccaaga ggagaggccc gacacggttt gtcaaaaatc gtgagggcga
      841 accaactagt gagtttactg tgattcttaa aaaagtggat gaaggataca ttgttataac
      901 tgcttttgtt ggcccgaagg cagagaaaga accctgggac ccgatggcca ccgaggagtc
      961 aaaagaattc tggaataacc atgcactcat ttgggagtca gaagaagtta ttcctggcac
     1021 tgagtccaca accccctaat atccaaggag actccttatc gcaaactgtg gataactgtc
     1081 gtggtagaat ctttgtaagc taatttaata aataaacatt catgaccaca gtaattatcc
     1141 tttctatagt tggtgcagtt atcgccgcag tagtaggcac cttctggtat tcgaacaaaa
     1201 ctcctatggg tcgtatccac atgcaatacc ttggcttcga caaactctct gacgaggaga
     1261 agagacagaa aatggaagaa gcaaagccaa agatgaccaa gatctatggt gcccagatgg
     1321 ctctatcgct gctcacagca tttgcagtct catatatcac tgttattggt atgggtagag
     1381 gtgggttaac tatttggatg gttcttggat ttgtcttgtt caattggatc tgcttcatcg
     1441 tgccaacaat tggtactcat atcctctggg gtaattgtga tcctaaaata gcttggaaga
     1501 agttcttctc tgactcattc tctgtactta caacactcat tatcaccaca ctactcgcca
     1561 gtctcttcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atgaaaaaat
     1681 tgattttagt attggtagta ctttcgattg gtaccctttg tttctttcaa tttaaaaaag
     1741 agccctatac tttaatacaa gattatagga agactaactc tgacgaattc aaatacttcg
     1801 gtaacgaagt agatcaggac ttaaataatg atggagtagt tgatacagca tttattgcta
     1861 cacaagaggt tagtggtcaa acttccttct acttaattgg cgcaatcaag aaagatggtg
     1921 gttatctttt gaccaagtct tttctgatag gagaaaatat ttctccccag acaacagaaa
     1981 gaatggaggg cggagtcgtg atcaattttg caatcggatc gaatccttcc ataggtaaga
     2041 gcctccgact agagctcaac tccaaaacat tagaattcgt tgaaaagttg tagtcccgca
     2101 ctatctttgg caaacaagga tcttcatcgg aagtttagga tatatttgta gaatatggct
     2161 cttataagct attagccaaa agtctatgaa aacaatcaaa tgttctgagg tcgggggaga
     2221 ttcatgtgac ttctcggtca ccacttctac aatacaggag gcaaggatga agcttttaga
     2281 gcacgctaag gaagcacatc cagagatgtt caagatcgcc acaccagatg caaggactga
     2341 atgggagaca aagtttaaga atgtctggga tcatgccgcc gagattgtgt aaattagaaa
     2401 aacacccatc tgggtgtttt ttggtataat aaattgatgg aaaatctcga cagaataatt
     2461 gtaaacaatg gcgggggact tggatcactt tggactatag gatggctctt taccataggc
     2521 tatctaaagc taagtttttg gaaaggtgtt ttagctctat ttatatggcc atattttatt
     2581 ggagtgcatt acagtaaaca tcatcaaaat aaggcagttt gagaatgaat tttcttcaaa
     2641 ctagtttaat agtgttagca gcatcatcag ttgctgttgc ggatattttc ttgaagaaaa
     2701 ctgagggtct gggcagtatt aacaaggcgc taagtagtcc ttggatgttt ggtgcactgg
     2761 cattatattt gtttcaaata atctttttca cgtacgcttt cgtatctggg tccaaattgg
     2821 tcaatgttgg tgttatgcag gttgcgctat attcaattat aatcttgcta gctagtttct
     2881 tcattttcaa cgaaactcta tctcaaattc aggttctcgg tctattgtta gcactttctg
     2941 gaatcatttt tatgcatatg taaaatgcct atttgggtgt aaaattggca aatgcataat
     3001 aaatttattc ctgtcattat tggattggtt gtggctgctt ccactggtat ttttgtgcaa
     3061 tcgaccaaga atccatcaat tccaaatcag gttccaacat ctgatgaagt tactgccaac
     3121 tattcatcca gtaaaacttt atataactac gacgaggatg aagacgaaga tgacgacgat
     3181 ggtgtttcgg gtagcactgt ctcaacccca gctactgaag cgacttcggc cactccacaa
     3241 ggcactactc caagcacggg tggtataaca cttgctcaaa tatcatcaca ttcatcgcgc
     3301 tcaagttgct ggtcggcaat taatggcgta gtctacgatc ttactagttg gattccaaat
     3361 catcctggtg gagaggggac aatacttgga ctttgtggaa gagatgggac acagtcttac
     3421 aacggtcaac atggcggtaa gacaaagata ttcaatatct taactggatt caaaattggc
     3481 acattagctc aataataatg aactattaca aacaacttac gaggcaagaa tggggcttac
     3541 tattacttag gtttggtttg gcaatagttt ttttatggtt tggattctca cagcttcttg
     3601 atggattaaa atgggtgtcc attgtcccag catgggcaac ggatttgttc aatattccgc
     3661 cggcaatgat tgtattagct aatggtctgt ttgaagtggt gttgggtaca cttctagcga
     3721 tgggtttttg ggtgagaata gtaagttttg tgttggctct tcatttgatt cctataactg
     3781 tggatatggg caatacagct acgggtgtta gggattttgg attagttgtt tcggctatag
     3841 cgttatcatt actctatagt aagaaaaaca cttcgatagc gatcgaaaat aaaaatcagt
     3901 attaacaaaa ataattaaac atatggaaaa aaaatatata tacggactag ttgggttagg
     3961 ggtgattgtg gtgttgtttt ttcttataga taatcttgcg tatgaaagtg ggcgacaagg
     4021 tattattgtt ggggaaacgg tatcaaggtc tggaaaaatt gtttcgattg atacaaatca
     4081 aattgctttc gacggaccat acatcttcag cctggagtct gaggcgggtg agttgaccac
     4141 agttgccata ccatctatgg ggttaccaat gtgtgaggca tacaaggcaa aaaagattgg
     4201 tgatgtgagt ttgattaaga tcagtgacga aatggaggtc aggggtgtgg ttagtgagga
     4261 tgggtcgatc gtaccgtgct catcaggtga acattatttt actcccaagg gaatagtggt
     4321 agagaacttc gaaggggagg cagatccaaa caggatgaag ttgactatga agacctggaa
     4381 ctgggttaac actctattaa acgacggcac tactattact cctaagattg agaataagtt
     4441 ttcaatcagc ttcaaggaag atggaacttt ctctgcagca accgattgca atggagtcgg
     4501 aggtaactat gtcactaagg acagttacat tgtttttagt cagatgatgt caactcttat
     4561 gtattgcgag gactcacaag agagtaagtt tactgagttt ctagctaata ctagtaactt
     4621 ccacttcacc tctaagggag agttggtgct tgatctaaag tttgacagtg gttctgttat
     4681 ctttcgataa ttgatacaga gtttcctgcc acaaaacccg tggtccaaca tagctgaagg
     4741 ctgtaacctc ctgatggacg atcgatattc aatacatcac caactacata caaattgctg
     4801 aataggcgag attgcatcgt cttgaaatct acttgagtaa ggtctatgcc accggatgtg
     4861 gtgattgcct tgtcctcacc taggagtcca agtactgaaa gatcaagatt cttcaaaaga
     4921 ttaattagtg agagtctttc ctctctggta acagaattgc attttttgtc tggatcaatt
     4981 ttcgataggc tcacaactat gggagctaga gaggttggga ccaaactctt taaactgttt
     5041 ttgaatttct ttttatcctc taatttaaaa atctcttgga gcttgctgtt tagcatcgag
     5101 tgatcaaggt cgggtaagat gtcgagagag ataactacct caccatactt gagtaattct
     5161 ccgacactgc tactcatgtt aagaattgtc ggtccactta accctacatg agtgaaaagt
     5221 attttaccct tagactcttc ttgcttcaca ctattttgta atagtttgat cttaaccttt
     5281 ggtaatgtaa gtcctgctac tcgtgccacc caatcatctt taacagcaat gggtacgagt
     5341 gatgcagatg gtttgactac cttgtgtcca gtgtcagtaa gccatttaaa tccgtctcca
     5401 gttgagccgg tctcggggtg agaggtgcca ccagttgcta agataactga cttaccacga
     5461 atttcttttt tattctttag tatgacacct gtaataattc cacgatctag taataattct
     5521 tggacaggtg agtcagatag cactgtgact ccgtgctttt taatatactc gaccatgaca
     5581 ccccagacag agtgagcttt gttcgataat ggaaagactc tttgctctgc ttccaccttg
     5641 gtttccatcc ctttaccgtt aaagaagtcc agagtctcct taactcccca cactgagaag
     5701 gccgagaaga ggaattgctc gctttcttta aacttggcta gaagttttct cacatcaggt
     5761 tcggcgttcg ttacattaca tctacctcct ccagtgataa ggagtttttt gcctaaagag
     5821 tcgttcttct ctactaaaag gactttggca ccgcgctctg cagctctacc agctgccatc
     5881 atgccagccg gcccgccgcc cacaactatt acatcccatt ctttattgta gatagaatcc
     5941 atccacttaa tctatcacag tacacaatat aaacctccaa aatagctaat tttgtgctac
     6001 tctactcagc tatggcaaaa gacggggtaa tactacgatt tgaagatcta agtttcgaat
     6061 atggtcagaa taaacctatt ttagaagagg tcagctttac tgtacgcgag ggcagcaaga
     6121 taactgtcat gggtcaaaat ggtgcgggca aaagcactat ctttggactc attactggat
     6181 caatcaaacc agatgttggg cacgtgtttc tcactcaagg tttgactgtt gcaatatcta
     6241 ggcaaactat tccaagagag gagctcaccc ttactgttcg agagtttttc gaaagagctt
     6301 ttcagaagtc atcagaggct aatcttcgta aggatgacgg caagatttat gacatagaca
     6361 agaggattga tgaggttttg gaggtggtga atcttaagag taagagtgat gtacatacta
     6421 ggataatgaa ttcattctct ggcgggcaac aagctcggct gctacttgct tcggccctca
     6481 ttcaagatcc ggatttactc ttgctagatg agccgacaaa taatttggac aaggcaggga
     6541 ttgagcactt aactcagttc cttaaagatt ataaaaagac tgtgatagtg atttcacatg
     6601 atgcggactt cttgaactcg ttttctgacg gggtgttgta cttggatatc tttactaaaa
     6661 aagtggaaca gttcgttggt aactactttg atgtggttaa acaaattgcc gcccaggtgg
     6721 agaaagaaaa caggaagaat gctcagttag aaaaggagat ccaagccaac aaagataagt
     6781 cgaacttttt tgccaacaag ggaggccaga tgcgtctggt tgcaagaagg atgcgagaca
     6841 aggctgcaga gctagaagaa agtattattg ataccaggaa ggaggacaaa accattagaa
     6901 ctttcgttat accagtgcaa gacggcgtga gcggagacct tttaactatt agcgagttca
     6961 ctgtacttaa cttgaagact cataagccag ttaagaggaa agcaaatatt aaactaagga
     7021 agaagcaaca cctactcctc aagggaccga acggtattgg taagagcaca ttgctcgagg
     7081 ctatcgccaa gggtgagatg gcggggacta atattactcc tggagttaaa gtgggttact
     7141 acagacaaga cttctccacc cttaacttcg aggacacagt ttatgattct ttaatgtcag
     7201 ttatggagaa gaagaatgag gagactatgc gcgctactgc ggcagggttc ctaatacacg
     7261 cagactctat caagagtaaa attggaagtt tgtctgaggg tcagaaaggg ttagtggcct
     7321 tcgcaagact agtgctcgag aagccggggg tactcatact cgacgagcct acgaaccata
     7381 tcaacttccg ccatttaccc gtcatagcag aggcgctaga taagtacgat ggagctatga
     7441 ttatggtcag tcacgtacca gatttcgtag aaaagattag gatagacgag gtcctagatt
     7501 tagagaaata gaggggtcta aatctaacct ttttgctata attagagata atgtatctga
     7561 agtcgataga actagctggg tttaagtcat tcgctaagaa gagtacgctc gagttctcct
     7621 cggctatttc ggctatcgtt ggaccaaatg gttctggcaa aagtaatgta gcagaggcct
     7681 ttcgctttgt cttgggtgag cagtctatca agagtctaag aggtaagaag ggcgaggatt
     7741 tgatatttaa cggtgcacaa ggtgagccga aggcaaatag agcttcggtc aaagtagtat
     7801 ttgataatca aaagaagctc tttggtttag attttagcga aatatctatt gaaagagttg
     7861 tccatcgaga cggtgttaat gagtacttga tcaatggcag tacagtgcga ttaaaggatg
     7921 ttatagagct tttggccaag gcacacatcg gtgcctcggg acatcacatc atctctcagg
     7981 gtgaagcaga caggatccta agtgccacag atagagagag gaagagtatg atcgaggatg
     8041 cgcttggtct caagatctat caatacaaga gactcgagag cgataggaag ttgaaaaaaa
     8101 cttttgaaaa tatcgcacaa gtcgaaggct tgcggaagga gatcgcgccc catctaagat
     8161 tccttaagaa acaggcagag aagatagaga aggtcgaagg actaaagaat gaactcttac
     8221 agctaagttt ggaatttttc aaaaaagaag aagaatactt gaaccacgaa aagaatctta
     8281 taaagagtga gagagagcct atcgaagaag ctttgaaaac acttcataac gaactcgcgg
     8341 aggctaagaa ggttttggaa gagagtgcgc aaggtaagga taagaacttg gagattctcg
     8401 agctcgagag tgaaattgca agagtgagat ctagcaagga tgcacttgtg cgagaacttg
     8461 gccgactcga gggtcttatg aatgcggagg aaagagcaat aaagaaagag gaagagttgg
     8521 cactaaacga agagtcgaag accgtgagac tcaaagaggt tgaagagctc ttcgaggaag
     8581 tgagtttgct ctcaactatc gagagtgtgt tcctaaagat caaagagttt atcaagagta
     8641 gaaagcaggc ctcaggtaca gacgcaattg tcgaagctag agagcagttg agtaggtata
     8701 aagataagaa gctacaaacc gagagcgagt ttgctctact tgaagccgaa gagacaaagc
     8761 ttcgtgataa atacgagagt ctaaagcgta agatcgagca agacaaggat tcatctcgtg
     8821 atgcagagaa agcggtgttt agaataatgt cggaggagaa tgagattcta agtcgaaaaa
     8881 atattcttaa aataagagag gagaggttgc atcacgatga cgaagcgttt aaaagagagt
     8941 tggccgaact tggtcatatg gtgggtgcgg aaatccttgc ctttagacaa aaggattatg
     9001 cgatggaggc agataggaac ttgcaagagg agaggaagag ggctcttgag agactaaaga
     9061 tcagacttga ggagtctggc gtgtctggtg ctggtgatgt gctcaaagag tttagggatg
     9121 tcacagagcg agacgaattc ctcgctcgtg agctcttgga tttggagaag tcagctgagt
     9181 cacttaagga gcttataaaa gacctagaga agagactggc agaagagttc tctcaaggtg
     9241 ttgccaaaat aaataaagaa tttagagaac tcttctctac aatgtttgat ggaggggatg
     9301 cgagtctcac tttaataaaa gaaaagaaga agaaaattgt accgagtgat actgatttag
     9361 atttaggagt agatgtgagt gacacagaag aggaggaggt tgaagagggt cttgagattg
     9421 ccgtgagctt gcccaggaag aagatcaggg gtcttatgat gctctcaggt ggagagagag
     9481 ccttaacatc cattgcacta ctatttgcaa tatcattggt gaacccacca ccattcataa
     9541 tcctagatga gacagatgcg gcgctcgatg aggcaaactc caagcgatac ggagacatgg
     9601 tagagaagct ctccaagcac tcagaactaa tactaatcac ccacaacaga gagaccatgt
     9661 cacgcgcagg tgttatatac ggtgtaacta tgggatcttc aggagtttcc aagctcctct
     9721 ccatcgcttt cgaggaagct gtcgaagtag caaaatagaa aaaagccacc aggttggtgg
     9781 ctagtcgttg tctgcctcgt cggggccctc gtactggtcg agaccagggc gagtgttgcg
     9841 ctgtgctccg atagtcgggt gagtcatgtc tcggagaccg agtacaggct gactcgacgg
     9901 cggagccttg cggtgggcga cggcgaagtt cagacgattc tcttctgcag tgtgctttcg
     9961 tgccaagact acccccggtg gaagtgatac tacaggccca agttctttga acaggcctaa
    10021 ataccatagt acaactaata actaatgtgt caatcaataa aaaaccacca gagtttaggt
    10081 ggttatattt gttgaccccc aggtgtgtgc aatttgatca aattgatcat ggcccttcta
    10141 tgttggacat gatcatctag gtcaaaaatg gtaatgaagt gggcagctcc gatttgggat
    10201 ctgatgaata cgcctagttg gcagtaatca gtgactttaa tccccactga ttccggtttt
    10261 ttggatatcg accaacgtgc agggatcacg tcaggtggag agaccttgat accatcccac
    10321 tgccaccctg tattgaactc attaattggc tgaatcataa tttcttcagc ccctccaatt
    10381 gtaacgacca tataccttgg cattggacca tccttgttga ggtgggcttt ctggagttac
    10441 tttaatgcct ttctgatcta agtcaaacga acttgaagtc tagaatcttt ttatccaaat
    10501 tagcgtctag gagttttact cgcactttgt cgcctaggga gtacttcttg ccggtctttt
    10561 ggcccactac agcgtattgc ttctcattaa gctcatagaa gtcgtcagtc atctctttta
    10621 gtcttaccat gccgtcagct ttggcagtag tttcctctac atacacaccc cactcagaaa
    10681 ctcctgagac aatggcgtcg aattcttgtc ccactttgtc actcatgtac tcgacttgtt
    10741 tgtactttat agaagatctc tcggcttcag cggcttcgag ctctctttct gatgatgagt
    10801 ctgaaagctt ctgatatctt gcaacctcgt ccgcctcgat cttgccctga gttagatacc
    10861 tctggagtag tctgtggacc attaagtctg ggtaacgacg aataggtgag gtgaagtgag
    10921 tgtaatactc gaaggcgagg ccaaagtggc cgatattcga ggtggagtag atagccttag
    10981 acatgctgcg tattgatgcc gttttgatca gcgcttcagc aggagtacct tcgaccgagc
    11041 ggagcatctt ggtaatctcc tgtgctgtgg tctcaccacc ttcgtgcttg agattaaatc
    11101 ctagtgcctt aacaaatgta gcgaggttaa tgatcttctc tctgtccggt gcatcatgga
    11161 ttcgatagac cacagctgcc ttagtattgc cattggtcat tgtgtgcatg tagcgagcta
    11221 cttcgcggtt ggctaggagc atgtaatcct caactagctt gtgtgtgtcc ttcctcacct
    11281 ttctaaacac ctttactggt ctacctgtgc tatccagttc gaacttaact tcgtccgatt
    11341 caaattcgat tgcaccattc ttaaacttct cttctcttag tttgtaggca atctgattga
    11401 gagtgttcaa ctcttcgtag aacattcctt cttttttatc caaaatatct tgcgcttctt
    11461 cgtaggtaaa tctcttgtcc gaattaataa gagtcttgcc gaaccatctc tccttcacct
    11521 cgccagaggt gttcattacg aacactgcgc cgaaggcgta cttgtcctcg ttcgggttaa
    11581 gagaacagag atcattcgag aggatctctg gtagcatggg gatggtgcgg tccactaggt
    11641 atatagaggt accacgcttt actgcttcgt cgtcgagagc tgttttagga acaacgaagt
    11701 gagagacatc tgcgatgtgc acgccaattt cataatctcc attatttatt ttctttattg
    11761 atatagcatc gtcgaagtcc ttggcatcag ctgggtcgat ggtgaaggtg gttgtatctc
    11821 taaagtcctt tctatcttta agatgatcct ccttggtcca cctctccttc cactccctgg
    11881 cttctttctc tacctcggtg gggaagtcga cctcgaaccc agactcataa acgataccaa
    11941 ccatctcggc gttgtgctcg ccagcgcgac cgatgatttt tataacctta ccgattgggt
    12001 tcttcgatgg ctcgggccac tctacaagtt ccgacacaac tttgtctcct tgtttggcac
    12061 ccatgctatt accagtgtct ataaacaagt ctcggtacat cctcttgtcg tctggtatga
    12121 caaagcactt gccattgtcg tcttcgaagg tgccgacgaa gtgcattttt ttcctcttta
    12181 ctatctcgac caccttggcc tgcttcctgt catagagctc cttgtccaaa agctcgactc
    12241 tgacagtgtc gccattaaga gccgtgttta ttgactcaga ttgcacctca aggtccacct
    12301 ctgtgtcagg aagtgcgaag tatccaatac ccttaccggt aaccttaatt acccccacta
    12361 gctcgtcttt catgtgggat aactatacca gaaccctgtt gaccgaacca ctcttttttg
    12421 ttactctact ccaatgctaa aaatgagact tcaaagagtg ggcaggaagc acgacaacgc
    12481 ctttcgcgtt gttttgaccg actctaagaa ctcttctaag tctggtaagt tcaaggaagt
    12541 acttggttct tacgacccaa gaaaaactgt cgacgttttg aagaccgaca ggatcaagta
    12601 ttggctcggc cagggggcct tgccaacagg tactgtgcac aacctctttg tgactcacaa
    12661 gattattgat gctaaaaaga agaatgtact cccaaagaag actccaatag ttaaggaagc
    12721 tccagttgta gaagagaagc cagttgtagc atctgcacca gctccagaag tagttgttga
    12781 ggctccagtt gaggtggctc cagaagtcgc tccagaggca tcagaacccg tggtagaatc
    12841 tacagaggta gctgaatagg ttacttaaga cactcttatt tccttagctc tgaccgatat
    12901 gctagacgac gtacagtttc ttgaattttt gataaaagca ctagttgacc acccagagga
    12961 tgtgaagatc aaacgaactg tggacgagat gggcgtactt atgactctgg atgtgaaccc
    13021 agatgatatg ggcaaggtga taggtagaca gggtaatact gctaaggcta tccgcattct
    13081 acttcgagta gttggtatga agaacaacgc tcgagtaaac ctaaagatca acgaaccagc
    13141 aggaggcaga acattcgccc ctcgagagga gtatacccct agagcttcta agacaattga
    13201 cgaagcgatg gaggatttga atctcgaata gtttaaatgc atttccatat aatcacactg
    13261 tttccaggta cattcgattc atactttgga gagtcgatac ttaagcgtgc tatagaggac
    13321 aagaagctca aggtctcctt ctacaaccct agagactatg ccgaagataa gtgggcgaga
    13381 gtagacagac ctccatatgc tggtggtcca gggatggtga tacaagccga gccagtgata
    13441 aaagctgtgg aggcggcaat aaagaagata aagagtaaga aatacaaaat aattttcctt
    13501 tctccgtcag gtaaacaatt tacaaacgag tacgccaaga agaccgccaa gacttacaaa
    13561 gacatcatca ttatctctgg ccgttacgag ggtatagatg caagagtgaa gaaggtgttc
    13621 aaaatggaag atgtatctgt cggacctttt gttcttactg gcggtgagct cccggcaatg
    13681 ctaatggtcg atgtaatatc ccgccaggta ccaggaattc taggtaattt tgattctttg
    13741 gaggaatcac gtactgcatc tcctgatgtt tataccagac cagagtcatt cacatacaaa
    13801 ggtaagaagt attctgtacc aaaggttttg ctctcaggta atcatgccaa aatcgaggag
    13861 tggagaagaa agaagaaggg ggtgtagaag aggtgctatt attggtatag aaacttctac
    13921 acggaggaca gatgagagcc aataggttta gcgtaatcat cggttcgggt gtggaaacat
    13981 cgagtacatg tatggctgca cccgtcgcag caaggcagat caccactgat catcatgaag
    14041 tgttgagagc gattttcgcc gctccaccga agaccgatca gctaatcgaa gcacttctcg
    14101 atcatcaaaa tgttttgagg gatcagtgac cgctgacata gcggtctttt ttatctagat
    14161 aatcaaaagt tatccacagt tttacgacaa ggggagggct tggtaagaaa aaaggtcacc
    14221 cagagttagt gggtgaccgg tgttctcgct ctactaatgg atgaaccatc ttgaaatcga
    14281 gtggtcataa gctgagactt cctggaaaac tctccaggcg aatgaccttc gagaatcata
    14341 agaaacctcg ccagtcgtga ggatcttaaa ggtccactcg tagtcatccg gatcgataag
    14401 aactacgaca gactcgaagt tcttcgctgc ggcacggaga agtgagggtc caccgatgtc
    14461 gatctgctcg atactcggtt tgctctcgaa atcgtagagg ttcaccacta caagatcgat
    14521 tgggacgata cctcgttctt tgatggctgt catgtgttcc ggcttgctac gatccgcgag
    14581 gatcccgcca aagatgttgg gatggagagt cttcagtctc ccgtccatca tctcggggaa
    14641 gccggtaaca gcctcgattg gtgtgaaggg tatgccgagt tcctcgagct tcttggcagt
    14701 accgccggtt gagacgatct cccagccgtt atcagttaga accttgagtt tgtcgagttc
    14761 tcgcttgtcg gtgactgata ctagtgcgcg ccttttcatt gggacctctt tgaggtggtg
    14821 gaacaagggt atctgtgacc aaactacctg atttaataaa actgtcaatt taactccaga
    14881 tatgatgaag aacaaagtta ttcacagttt cttacggagg ggagggcttg gtaaggtggg
    14941 tggggtaagt ggggttgaca ccttgcttag gcttatatat acttccggta cttcgaaacg
    15001 attgagtcac gaccaaagaa ggcagtaata gggagaatta ttagcctctg tttgtaaatt
    15061 taaataaaga atgtcgacaa aaacctcggc tagaccgcag aagttcttcg gtcgtttccg
    15121 agagccatta accgagatgc caaatctcgt ggtctctcaa gttgaaagtt ttaagtggtt
    15181 gattgagaag ggtttgaagg aggtgttcga ggagttctca tctataaagg atttctcaga
    15241 aagaaaattc caactagatt tcgttggatt tgaactagat aagccaaaga tagacgaata
    15301 cgaagctaaa gaaaagaagc tctcttacga ggcgcctctt cgcgtacgta tcaaactaaa
    15361 gaacttcatt cagaagacag agaaggagca agagatcttc atggcggact tcccactcat
    15421 gactgaccac ggtaccttca ttatctctgg tattgagcgc gtagtagtgc ctcagttggc
    15481 tcgttctttc ggtgtattct ttaccgcaca ggacgttaaa ggcaagaaga cctttggtgc
    15541 caaggttatt ccaggccgcg gagcatggat cgagtttgag accgatgccg acggagtgat
    15601 taacgtaaga attgatagaa agaggaagtt cccagtgaca gtactcttcc gcgcatttgg
    15661 tgctactact aacgagaaga ttctccatct ttttgacggt cagccagagg ccaaggccta
    15721 tatagagaag actctcgcca aggaccacac tattactcag gatgatgccg ctattgagat
    15781 ccacaagaga ctcagagacg gtgacctagc ctcagcagag aatgctaggg agttcgtgaa
    15841 gtcgatcttc gacgcagagc gatatgacct ctctcgtgtt ggacgattcc gcttcaacaa
    15901 tcgtttcgat cttaagcaga gtgataagga gatggacaag agaaccctct ctatagaaga
    15961 ccttagaatc attattgctc atatcatcac tcttaatacc actcccaatg cacttccaga
    16021 tgatatcgat cacctaggaa gccgaagagt aaggtatgtc ggcgagctct tccagcagaa
    16081 gatcagagtc ggtatggccc agatcaagag aaacattcaa aacagaatgt ctactattga
    16141 taccgacgtt actctcccag tacagttcat ttctcctaag ccacttcagg ccaggatgaa
    16201 ggagttcttc accacgaacc aactctcaca gttcatgcaa caggaaaatg ctttgaccga
    16261 gatcgaacat cttcgaactc tctcagcact tggtccagga ggtttgaacc gcgctcgtgc
    16321 aggatacgag gttcgcgacg tgcacccatc tcactacggc agactctgtc caatccacac
    16381 tccagaaggt ccaaacattg gtcttattct tcgtctatct tcttatgcga aggtgaacga
    16441 cttcggcatg atcgagacac cttacgcagt tgttaaggac ggcaagatta catcagagat
    16501 caagtaccta aacgctctag aggaagagca atacaacatt gctcatgcag cgacaccagt
    16561 tgaggacaac aagattgtta acgaagttgt ggatgttaga tacaagggtg agcctcactc
    16621 agttaagcgc tctcaagtgg actatatcga tgtagctacg aaccaggcgt tctccattgc
    16681 tacttctatg atcccattct taaaccacga tgacgctaac cgatcactca tgggttcaaa
    16741 catgcagaag caagcaaccc catgtatcat ccctgaggcg ccattagtag ctaccggtat
    16801 cgaggagaag gcagttagag acacaggcag gctagttatc gctcctgtag caggtactgt
    16861 cacatatgtg gacggtaagt tgatcattct tacagacaac aagggagaca cacacgagtt
    16921 caaacttgtg aatttctcaa tgacaaacgg ctttacctca ttccaccaga gaccatcagt
    16981 tgaggtaggt caaaaggtta agaagggaga tgttttggca gacacatcat cttcggagca
    17041 cggtcagatg gcactaggac aaaacattct cgttgccttc atgagttggt caggtgcaaa
    17101 ctacgaagac gccatcatcc tctcagagag attggtgaag gagtctaagt tcacctcagt
    17161 acacatcgag gacttcattg tgaatgtccg cgacaccaaa ctaggtcccg agatcactac
    17221 ttgtgacatt cctaacgttg gcgagagtaa gctaaagaat cttgcagaag acggtatcat
    17281 ccgcgtaggt gcagaggttc gctcaggcga catccttgtc ggtaagatta ctccaaaggg
    17341 agagagtcag ctcactccag aagagagact cctaagatca ctctttggtg agaaggcaag
    17401 agatgtgaag gatacaagta agcgtatgga aggaggcaag cgaggacgca ttatctcagt
    17461 ccaagtgttc tctcgtgaga gaggagacaa gctagatagc ggtattctaa agcagatcca
    17521 catccaagta gcacaattaa gaaatgtttc tgtcggagac aagctcgcag gtcgtcacgg
    17581 taacaaggga gttatctcaa gaatcctccc agtagaggac atgccataca tggaagacgg
    17641 tactccagtc gacatgattc ttacaccact tggcgtgcca agtcgtatga acctaggtca
    17701 gatcttggaa ctccacctcg gtcttgcagc aaacactctt aactaccaag caatcgtacc
    17761 tccattcgta ggagcagaag agaaggaaat taaggaagaa cttaaaaagg ctggattcaa
    17821 agagaacggc aagatgaagc ttttcgacgg tcgctcgggc gaagccttcg accaggagat
    17881 tgcagttggt tacatgtata tcttgaagct ccatcacatg gttgaggaca agatccatat
    17941 gcgttctatc ggtccatact ctctcattac ccaacaacct ctcggcggta aggcacaaaa
    18001 cggaggtcag agattcggag aaatggaagt gtgggcactc ttgggtcacg gtgtgggaca
    18061 tactcttcgc gagatgctta ccatcaaatc tgacgacatc cagggtcgct cagcagcctt
    18121 cgacgctatt gtgaaaggcg agagaatcca ggaaccaggt acaccggcat cattcaacgt
    18181 gcttgtgaac aatatgagag gtctagcact tgatgttgat gtgctcaagt caaggcagga
    18241 tgaaccaact aaagaataat gaccatgaac aacaacagaa aaacaatcga cactaaggac
    18301 ttcgatgcaa tcggtatccg attggcaagc cctgagcgta tcaaggagtg gtcatatggt
    18361 gaggttacca agcctgagac catcaactac cgaactcaaa gaagtgagag gaacggtctc
    18421 ttcgacgaga agatcttcgg accagacaag gatttcgagt gttactgtgg aaagtacaga
    18481 ggtattagat acaagggtat tgtctgtgag aagtgtggag tcgagatcac tcgctctata
    18541 gtaagacgcg agagaatggg tcacattgag ctcgcgagcc cagtggcaca catctggttc
    18601 ctaaagaatg tcccatcaag aatctctctt atcatgggta ttccagtgtc cgatcttgag
    18661 aaggttattt acttcgcagg gtacattgtt acctcagttt ctgagagtga gaagaataga
    18721 gtgcttaagg acctagactc tgagttcaag agcaaggtta agtctcttca cgacgacaaa
    18781 gccaaggagg cactcaagga actagctcag caagctaaga atgatatcga gtctctcgtg
    18841 ccaggcagag tcttggatga agttgagtat cacagattcg gcatgaagta ttcgacaatc
    18901 tttgaggcgg gtattggtgc tgaagcaatt ttcagtatct tgaagtcact cgacttaaag
    18961 aagctccatg cagagataga cgaaatgatt actagagcac cagctacaga gctcgctcgt
    19021 ctacataaga gagcatctac agtgcagtgg atgataacct caaacattcg ccctgagtgg
    19081 atgttcctaa cacgtattcc tgtcatacca ccagccattc gcccaatggt gccactagac
    19141 ggtggacgct atgctacatc cgatgtgaat gacctatatc gtcgtgtcat caaccgcaac
    19201 aaccgtttga agaagctaaa ggagatcaat gcgcctgatg ttattctccg aaacgagaag
    19261 agaattctcc aggaagcagt agacgcccta atagacaaca ccattcgtca tggtagtgct
    19321 ggtggaagtc aggctcagag gagaccacta aagagtttgt cagacaacct caagggtaag
    19381 cgtggactct tccgtcagaa cctcttgggt aagagagtgg attactctgg tcgttcggtt
    19441 atcgtcgttg gtccagaatt gaagcttcac caatgtggtt tgccaaaaca catggcacta
    19501 gagctcttca agccattcgt tatctcagaa cttttgaaga aggaacttgc ttacaacatt
    19561 cctggcgcaa gacgtcttat agaagacgca ataccagagg tttgggcaat tctagaagac
    19621 attattaaga acaagtatgt actcctaaac cgcgctccta ctctacatcg cttgggtatt
    19681 caggctttcc aaccaatctt gattgaagga aatgctatcc agctccaccc actcgtatgt
    19741 actgccttca acgcagactt cgacggagac cagatggcag tccacgtgcc actctctata
    19801 gaggcacaag cagaagctcg tctcatcatg gcagcggata gaaacctact aaagccaggt
    19861 aacggtgagc ctgtggtaac caacaagatg ctcgacattg tccttggtac ttactggatg
    19921 acacgtatca ttgcgggcga gaagggcgag ggttcttact accagagtcc aaacgctgct
    19981 atcactgcac acgacttcgg cgcagttgaa tttagaagca agatcaaagt tcttgcgact
    20041 gattctccaa agtacaagca cttcgagggt aagcttttcg aaacctctgt aggtagacta
    20101 ctcttcaact caatacttcc aactgattat cctttcatta ataatgagat ggacaagaag
    20161 aggatgggtg agttagttga tgatcttata gagagatacg gtatcgagaa tctcccaagc
    20221 attgcagaca agatcaagag cttcggattc aagtacgcaa ctaaatcagg agtaacatgg
    20281 ggactagacg atgttaagat cccagaagag aagccagaga tcgtagaaaa gggtagaaag
    20341 gaggtcaaca aagttataga gcagtggaac gatggactcc tctcagaaaa tgagagaatc
    20401 cagaagtcag tcgagatctg gcaagctacc aagcaagaga ttgagtctct catcccaagg
    20461 actttggcac ttaaggactc tgtgtatgac atgacttact caggtgcaag aggttccttc
    20521 ggtaacctaa accaaatggc cggaatgaag ggtatcatcg cgaatgtctc gggtgagtca
    20581 ctcgagttcc caattacttc ttgttacaag gaaggtctaa caccacttga gtacttcatt
    20641 accactcacg gttcgagaaa gggtcttacc gacacagctc ttaacactgc taaggccggt
    20701 tacctcacac gtcgtctctt cgatgtcgca caagatgaga tcgttactga agatgattgt
    20761 ggaacaaagg aaggtatcgt tattaccaag gagagtgctt ccggtattca gatcccactt
    20821 tcaaagtcag tgaagggccg cttcttggct aaggatgttc tagatgcaac tggccaaaca
    20881 ctatttaaga ccgggcactt cgttactcgt gccgatgcag atcagataga caaggctaac
    20941 ctcgcaagtg tagaagtacg aagcccaatg atgtgtaagt cagagcacgg tctatgcgtg
    21001 aagtgctatg gtgcagacct aggcaacaac aagattattg aactcggcga agctgtaggt
    21061 acagttgctg cacaagctat cggtgagcca ggtactcagc taacaatgag aaccttccat
    21121 gccggaggag tggcggctgc aggtggagac atcactgccg gacttccaag agttgaagaa
    21181 atctttgaga agaggaagcc aaagagtcca gctgttatct cgcatgtcga tggtacggtg
    21241 actgagatca aggtctctgg tcatgacaga attatcgtag ttctacctga gatcggtgag
    21301 actaagacca agggcaagaa ggttgacact gagtacacag taccaagcgc tcgaaccatc
    21361 tttgttaagg ttggtaactc ggttaagaag ggtgagatgc tcacagacgg atctgcagat
    21421 ctagacgagc tattcaagta tgctggtcga gacattacct ctaagtacat cattcaagaa
    21481 gtttcaaagc tttacgaact acaaggagca gcggtgtcac acaagcatat cgagctcatc
    21541 atcagacaaa tgtttgcaag acgaagagtt aaggatccag gcaccaccac tcttactcgt
    21601 ggcgacattg tcgagatcag tgaggttaac aacactgatg tcaagtcaga ctcagtaatc
    21661 atgggtattt ccgaagtctc actctctagg tcatcattcc tctcagcagc atcattccag
    21721 cacacaccaa gagtacttat ccaagcagcg ataagaggtg caaacgatgg actacttggt
    21781 ctcaaggaga atgttattat tggaagactg attcctgcag gtacagggtt taatggtggg
    21841 tacaaacaaa aactcatatg ggaggaaacg tcgaagcaat taaagaacgg cttgatatag
    21901 ccgaggtcat aggaggatac atcaaactcg agaaagccgg agcgaactac aaagctaaat
    21961 gccccttcca caacgaaaag actgcaagct ttcatatctc taccagtaga cagggctatt
    22021 attgctttgg ctgtggggcg aagggagaca tctttacctt cgtgcaagag atggagggga
    22081 ctgacttcag aggtgctctg aaaactctgg ccgagagagc aggtatagaa ctaaagcagg
    22141 agaggagaga ggcgaaaggc gagacagacg aactctatca agcgctcgag gtagcaactg
    22201 agttcttcga aaagaactta gcaaagaata aagaagctct cgagtatctt aagggtagag
    22261 gagtgagtgc tgaaagcatt aagacattcc gactaggact agcgaatgat gcttggagag
    22321 agcttcgtga acacgctaac tcgcttggta tcaaagacga tgttctttta aaatccggac
    22381 tcatcaaaag aagcgaagag ggcagaagct cggagcctta cgataccttt agagggagaa
    22441 tcatcttccc tctaagtgat gctagaggta aggtaatagc tttctcaggt cgtgcgttag
    22501 cgaaagatct tgagccaaag tatttgaaca gtccagacac accgctcttc aacaaaagtg
    22561 aggtactcta cggcttcgac aaagcgagag aagagatcag gaagaagaac tatacagtct
    22621 tagtagaggg gcaactagat ttagttcttt cgcaccaagc aggagtgaag aataccgttg
    22681 catcatctgg cacggccttc acccaaatgc atttggagaa actgaagaaa atctcgcctc
    22741 gaataatcct tgccttcgat aatgatgatg caggaagcaa ggcatcgtac aggagtacgg
    22801 ctctagcact ctccttaggt atggaagtaa agattgcaga aataaaggga ggtaaggatc
    22861 cggctgatat ggtgaaggag gatgaagaaa gttggaagga ggttcttaga aattctaagt
    22921 ctgctgtgga gagttttatc tctgctttgg tttctagaga gtcgagtctt gcaaaggttg
    22981 gtaaggaggt agagaacaag atccttccac tcatagcatc gatgacctca agattcgaca
    23041 aggaggacgc ggtgtcaaag gtagcaagtc ttataggtaa gagtccggcc aatgtgggag
    23101 agatggtgat gaagatcaag cctgaagttg ttcactcact tatcaagact gaagtagaaa
    23161 gtgtcgtaga agaaaaggaa gtgatgagtc gagtagaaga gttagagaat gagattaaaa
    23221 ctatagaaga acttcaaatc ggtttggata aggggacaga tgaatacaaa agatcagagc
    23281 tttctaaaaa ggagcttaac tctcatctga aaatatcaaa actagagggt gagaggaacg
    23341 agttgaagct ccgtgtagca agaggtggag gagaggagtt actacacgag atagtagagt
    23401 tgggtaaaaa aatagatgaa gaaaggagaa atttattgta gaatatccaa attataatgc
    23461 caaaagcaaa aaagcaaaag aaaacaaagg ccaaaaagat aagcgtcaaa ccgcgagtca
    23521 aaaaagtgaa ggccaaaaaa gtttctaagc ctaaggtcaa gaaagcaaag aaagtagtag
    23581 ttaagaagaa agtcctctca aaaaagaatc aagaaatgga gacgaagatg aatcgtctca
    23641 tcgctcgtgg caaggagagg ggctttatca cttatgacga aatattgaaa gagttcccaa
    23701 atatcgagat taacattatc tttcttgatg aactctatga gaagttccag caagctggag
    23761 ttgatgttct tgaaggcggt ggactcttgg aaatctctaa tccagatgat gacaagaagt
    23821 tctactctaa gtcggactct gcttatgact ctatacaaat gtacttgaag gagataggtc
    23881 gctacccact catttccggc aacatggaga aggaacttgc caagcgtatt gaggaagggg
    23941 atgaagaggc taggaactta ctcgcgcaag cgaacttaag actagtggtc tctatcgcta
    24001 agaagtatgc gaacaggagt ccagacctta ctctgctcga cctcattcaa gagggtaacc
    24061 tcggcctata caaggcagtg gagaagttcg accg
//
LOCUS       DAORIT010000004        21542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_384_length_21542_cov_48.553637, whole genome shotgun sequence.
ACCESSION   DAORIT010000004 DAORIT010000000
VERSION     DAORIT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21542
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_21542_cov_48.553637"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..4724
                     /locus_tag="PLD99_00635"
     CDS             99..4724
                     /locus_tag="PLD99_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25446.1"
                     /translation="MLSGAATGCLTSTNGWITSTGSACGGVGGSSFSFPWTTLGTGEN
                     ATSTILSLQGGFLASASSTLASTTVTTLLANSATTTSLFVSDLASTTALRANTANIGN
                     LTVESCTGCGGAGGSLQGSYDSGATIETASNVAVVITETSAAANTGDLLQLTANAATG
                     GTFSGDALQITMDAVDANGYTGNGLRIVVDQSQNTGSPILIEDDAGVDLLAVSETGGL
                     TVGSAANGRSDINVYGDLITKGYNVRKSLANIIDLFVYDTTRDSDRGHWRTSLNVQGT
                     SWYTETKDDGPSDACVIATDDRCGSSEFPRTAIIATTADGVYIFDAKDNSLWMKFTQA
                     GTYALGADTNNNPSGVSAVNGVVYVGTNGASATGMYAFDFAQDKMYRYNATNRVEADT
                     TIGTRNSATSYAGDPITALAILNATVNDVSAALMNAGADAFANVVTLLTDAGAGPVKG
                     LVKIAVANDSGVSVVSPVEQKVFNYSDVANDDYNTVFLTSRGRMYAANETQAQLESWR
                     NVDRDNTSEVAGTPDKIYDEVVGRAPITNGVAPTILTSPATLAVVERASGALESAAAG
                     VVDVGDLVYFGTNQGFVEIQDSGGSTVGANADGWTKITTTGTSTPWMHGPVRSVFLFD
                     EVTGSTDAVSAVGGAGTGANNRLDAANATNPTFSGNGGVRGGSVNFNNNSYFCSDANN
                     DGTCDNDTDYNVAAISFTISLWFKHSTTAAADTLFEKCFVPATGVAAGACVAAGMNAS
                     GQITFGIDDDTTWATGSTTVAMDDAITSTNTFNDGRWHHAIFTNTDTDICMYIDGRQA
                     VACDSTLAATATLDTGSATLTIGGQCSAAACATGVAFWDGEVDDFVWSSNLNTTIGGM
                     NAEAARKLYLSSAQEREVRMASTTDATTFSSNTIGDSGESWRVNEFTGRMVTIVDGTG
                     IGQTRRVISNTATVLTVFPNWTVTPDATSDFKVDPSRLYGYSDNVTSVAVDLPQALNT
                     QRNSVYVGTSNGSDGGGVTVFTNNLVTDYYHTDAGLSSDDFGTAWSGTDADDIQAIAA
                     YNGTIAIGSLATINVVKQDKSFEQTRLETIGALNGIQTELISKHLFGSSMESFGVGAG
                     ADLAEYYSSTQPLERGEIVTLDPTNPHAVLRATKPYQSEALGVIATQPGIILGPYVDT
                     SFPVALVGRVPVKVTTENGVIKSGDRVTSATLSGYGMRATKSGRVIGVALEDAKIDDF
                     FECPDDPQGVAERRCGQVMVFVNLVDYNGESIDMLLSEIEEGVDFVSADITATTTATS
                     TTSTLDQETELDKDVRVIKLLKSINVTGQIDAEILTDRLSAAFEIYSPRIITEGLRVD
                     YVSALFEEIGFKSDVVFFGRPYFTTDTAGFALVKAGDIRVDVVFDREYLETPVINASM
                     SSKDGLSSVNQSVAIQAFFGKDIKFLITNATTTGFTIYLNKPVDEDVSFNWIALAVKS
                     AKMFESSGSSTPVNVVDEDQSPVQEDEPVVGETNDLMEESTPVNVGDSSTTTPEILPL
                     NEVPVATEDSTTPPVEELAPEEVVVVDVPVTEESIIELPVAADEPQQ"
     gene            4708..5688
                     /locus_tag="PLD99_00640"
     CDS             4708..5688
                     /locus_tag="PLD99_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HRH25447.1"
                     /translation="MSHNNKKLIILVPVVFVLGLVFGHLINFGEIVNDSREIRMDDGG
                     LTNPLLECEVAEDTIASRKVDFTPKLKDFISDMSLRGVNNISVYFRDLNNGPIVGVGQ
                     DNSFAPASLLKVPVLIAYLKWSEDKTGLLDEVVLFEESKGIDYEQVFPPEENIVVGES
                     YTVRELLERMIKYSDNQALLLLHKRIPIKYQEELYTLLGVDKSIITDPSGRITVRQYS
                     IFFRILFNSSFLSRSNSEYALKLLSSIAFDEGLRAGVPDKVLVSHKFGERQTSANEQQ
                     IHDCGIVYYPNHPYLLCVMTRGEDVKSLVEGIKNVSAFVFGIIDEQYRAI"
     gene            5727..6107
                     /locus_tag="PLD99_00645"
     CDS             5727..6107
                     /locus_tag="PLD99_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25448.1"
                     /translation="MSRVIHIIKNNIVLVLILLLVVMTGASIYLYNKSARLSADPNVK
                     AEEEVARLVERVGKLIYLPEDETPTMATVSDPELLKDQPFFAKARVGDKVLLYQKSKK
                     AYLYDPKSNKLLEVAPINIGTEEK"
     gene            6115..7416
                     /locus_tag="PLD99_00650"
     CDS             6115..7416
                     /locus_tag="PLD99_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25449.1"
                     /translation="MKIKYLHLTLFILTFIFVGRVGAQEFTSTNFKILDPVVNPGEYS
                     SSASFTLSSVLTQLGIGTSSESTYNLFGGFLYFPFITTPTLSATPSSGQVSLSWTAAD
                     GVLGWSAGSYSVGEGTVSGGPYTYTNVGNTLSTIRSGLTNGTSYYFVIRVLDTLGNVI
                     ATSTQVSSTPVAGGGGGGGGGGGGGGGGGSAGVRFSGRAYPLSRVSILKDGQIAVTTI
                     AGPNSNFAATLSDLASGNYTFSVRGEDKNNLVSNPFVFPIFITENVITEVGGIFISPT
                     ITTDKLQVKKGDNIVIFGQTANNADVLISINSEPEHFVTRKSDKDGIYLLNFDSSILA
                     LGDHTTKSKASTEDEISPYSSSVSFKVGNENILSGPVTCQMKADLNNDCRVNLVDFSI
                     AAFWYKKSLSTTFKEVERTKLNADNKIDLVDFSIMAFHWTG"
     gene            7419..8288
                     /locus_tag="PLD99_00655"
     CDS             7419..8288
                     /locus_tag="PLD99_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRH25450.1"
                     /translation="MKKLKVLIVSLVLLVPVITFGAEVSLVSKDKEYVVNKEFTVNVY
                     LDTEGDSINAVEGDISFKPSVLEVVAIKDGNSSVNFWINKPGLVTKDKISFSGVTPGG
                     VRGQDRLLFSIVFKPIKSGESNVDIGDVVLLKNDGLGTKASTKVVGLSIKVVPSTGGP
                     ATNEVVIDDREPETFLPVIGRNSEIYEGKSFLVFSTQDKESGIEYYEVKEGIFGEYTR
                     AISPFVLNNQKLDKKIYVKAVDLNGNTRVVSIKPENYKVWYKRSEILGIIILVILVVF
                     YNRKKWFNPAEKL"
     gene            8261..9370
                     /locus_tag="PLD99_00660"
     CDS             8261..9370
                     /locus_tag="PLD99_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRH25451.1"
                     /translation="MVQSCRKVVALVTVLLLAPVFVSAATLSISPQSGSYEVGDRFTV
                     RVVMSASEAINAVSGELKFPTSVFAIDSVSKFSSILNFWVSEPSYSNTLGKVNFEGVV
                     LGGFQGGTGTVVTLNLRAVSVGSGRIDFVSGQVLANDGQGTNVTQGTTGANFSIVEAT
                     KPKPVEKKVEVVEEVAKPDLAAPEIELVSKYGERAVSGISSYPKSEVVLTFVNEVGVK
                     IFILGSTDDSGKFLLLVPKSLKRGEYSVSAQVVQKGSSYSAVSNTIKIKIGNIISDIG
                     WGARIAFILLILALIYVSYRLRMYFVKNSKMKYEAGEVKDVVKKSFKILEEDIENSKD
                     NAKNAKDREGLSNTLKDLSEAESVILKEIKDIEKL"
     gene            9367..10707
                     /locus_tag="PLD99_00665"
     CDS             9367..10707
                     /locus_tag="PLD99_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRH25452.1"
                     /translation="MIKVSKWSSLLVLALLLSWSNVRAQSISVDASRLLRKPEIILSP
                     RTGSFVEGSTFDVPILLNTNGTSINSLEVRLNFDRNKLEIVRPTGGESIIGVWVEPPK
                     FDNKNGTASYVGVITNGITTNSGLIGTVTFRAKSAGRATVYIGSDSKVLMNDGLGTEV
                     ESEFGRADYSILVRPPEGVRVFSETHQFQGQWYNNNNPSLSWDKDVGVDGFSYILDNK
                     PSTIPENKIVTTETTTSYENLADGLWYFHIKARKNGAWSATGHFLVRIDTKPPALFKP
                     EVAYLTKDKIADRTLISFVTTDNLSGVDRYEVGVIDKADSSNTSPLFVEVESPYQVPI
                     VGSGYLNVIVRAIDGAGNVVDVSLDIEPQTFASSFIKSNWLSVLLIILAVLITGFITN
                     YFVGHHVLRHFRRAVEFIKRDTATSDAVLSPPPPPPLFPPINDNISKNGNEPNK"
     gene            10691..11059
                     /gene="msrB"
                     /locus_tag="PLD99_00670"
     CDS             10691..11059
                     /gene="msrB"
                     /locus_tag="PLD99_00670"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRH25453.1"
                     /translation="MNQINNNLTEEEKRVMFEKGTEAPFTGKYLQSKESGVYVCKNCG
                     TELFTSDAKFDSGTGWPSFDNPANTKNVILEEDNSYNMHRTEVRCAKCGAHLGHVFDD
                     GPTGTGKRYCINSVCLDLQK"
     gene            11179..11463
                     /locus_tag="PLD99_00675"
     CDS             11179..11463
                     /locus_tag="PLD99_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRH25454.1"
                     /translation="MSTKLYVGGIPYTSTEQNLKDAFGKAGNVLSASIIIDRMTGRSK
                     GFGFVEMSNDDEANAAINMWNGQDFDGRKLTVNVARPLEPRAPGMNRRPM"
     gene            11531..11977
                     /locus_tag="PLD99_00680"
     CDS             11531..11977
                     /locus_tag="PLD99_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRH25455.1"
                     /translation="MYKKVLLLVVLSLLLFISLKYLNPGCDCNVTERVTIGGTEMQAF
                     VSDTEDERSQGLSGKEGLGEGEGMLFVFEYPAKYGFWMKDMNFPIDIIWIGAEKRVLA
                     IEKGLEPSTYPKVFYPPDKVLYVLEVPSGFSDTAKIEVGQSLSIGQ"
     gene            12140..14779
                     /locus_tag="PLD99_00685"
     CDS             12140..14779
                     /locus_tag="PLD99_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRH25456.1"
                     /translation="MVIYVTRRDGTKEPWNADYINKSIERETKGLADPIAKVIQIAND
                     TRLTVYDGITTDEMDQATINAAVQNIKDDPAEYDKIATRLLLKTIYKKVVGHYNSDDK
                     ETLKVRHREGFIKFVIEGVEQGRLHPNMTAKFDLERLSHALKIQRDDLFLYSGLDGLL
                     NRYGMKDISQRPLETPQYIFMRIAMGLSYNEADPTAWAIRFYDKMSKHEYIAGGSTNL
                     GAGTVRAALSNCFLLEIHDNMEHIAKSVSDVMLLSKGSGGIGASVTKLRAAGSPLKSN
                     FGGVSTGPTTFAKIIDTAIRAIQRGGKKKGALAFYMENWHLDFPEFLDWKHNAGDDYL
                     RMRTANTAAFISDEFMKRVQAGDEWYLFDPAETPDLNETYGKVFSKRYAEYIEMANKG
                     EIRAFKKMPARELWRKTLVALQTTAHPWITWKDPINLRALNNNTGTIHMSNLCTEICL
                     PQDKDNIAVCNLTSLNLAAHVSEKQVNWQKLEESVRLAVRQLDNLIDINKLPVPEAEK
                     SDKENRAVGLGVMGLADVFERLGLAYDSEHAYNFTDRIFEFVSYMAIDESANLAAERG
                     VYKNFEGSRWSKGMVPFDTLEVLEERRGVPLTVDKTSHHRGLDWNILREKVKKGIRNA
                     TLMAVAPNANIGLVVGTVPGIDARFSQVFSRNKISGKYLDINHNLVRDLKNMDLWEKV
                     KGMIIELQGDISSIPEIPDHIKAIYKTSFTTSPYAYIEVAARAQKWVDQALSRNMYLE
                     TRDIDETMHLYETAWEKGVKTTYYLHMKPRHTAEQSTVAVNKAVTMGKRGFAGLSSSP
                     KVEVEVEGSKVPVWNQKEIQPKLIDATPVRKIEQPVHSGQVVRVENRETEKVEVRPVS
                     QTSKLTVHLPDDPANANICDDCQ"
     gene            14796..15860
                     /locus_tag="PLD99_00690"
     CDS             14796..15860
                     /locus_tag="PLD99_00690"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRH25457.1"
                     /translation="MLVGKASPDDMNLHPIRYQWAYDLYNQAVRNTWFPHEIALKEDL
                     DDWAKMTEDERHAVKFLMAFFNPAELIVNRSIALGIYPYLKSPECHLYLAKQMWEEAN
                     HCVAFEYVLETFPFDRENIFNLHLSVPSMTAKEAYINKYMKRMTEDSLDIDTMDGKKD
                     FIRNLVATNIVMEGVWFYSGFMVALSFRQRNQLRNFGSMINWVLRDESLHLKFGMNLV
                     HNILEENPELLTEDFANEIRDIVVEGVNLETAYNKDLFPQGILGLNADYVNQYVQYVA
                     DRRMEELGLPKYYNATNPAKWMSTATDVYELVNFFEAQNTSYEVDGHSATVKKSGSSG
                     QSEEPRKAPDAPTIPTGVGE"
     gene            15881..16912
                     /gene="gap"
                     /locus_tag="PLD99_00695"
     CDS             15881..16912
                     /gene="gap"
                     /locus_tag="PLD99_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRH25458.1"
                     /translation="MKIRVAINGAGRIGRAFYKLARENKKLEVVAMNDLGDIANIAYL
                     LKYDTTYGQSGLKIEVKPDNSALIIDGEEVKFVQVREAKDLPWRDMDIDVVVESTGLY
                     TSYEKAKAHLDAGAKRVVVTAPMKDDPIPGIEGATVLMGINDDKLATCQITSNASCTT
                     NAASPVLAILDEALGVEKAILNTVHSYTASQSIVDGPNKKDWREGRAAAQNIVPSSTG
                     AAIAVGKALTQLDGKFDGISIRVPTVVGSIADITFIAKRDTTVEEVNKIMTEAAKTDR
                     WKDIFEVTDEEIVSSDIKNAAHASIVDLAMTRVVGGNLVKVLAWYDNEMGYTHTLVQH
                     VVKSGSQIK"
     gene            16912..17388
                     /locus_tag="PLD99_00700"
     CDS             16912..17388
                     /locus_tag="PLD99_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRH25459.1"
                     /translation="MKIFVGSDHAGFGLKQKMIPFLQSLGYEVIDKGATEYDEADDFP
                     DFISQVAREISMNPNNAKGIVMGGSGQGEAMLANRFKNVRATVYYGGGKSLVEDNDSI
                     IKLSRDHNDANILSIGARFVTDDDAMRATHEWLETSFSEDERHKRRIAKFDRVTHE"
     gene            17336..17941
                     /locus_tag="PLD99_00705"
     CDS             17336..17941
                     /locus_tag="PLD99_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25460.1"
                     /translation="MKDIKGELLNSIELHMSEVLPAILPDSAQDLVDKVALLPKEIEI
                     LHLDVMESDVWAPIGKDFEAHLMVEYPEDLIERWIDRGAKRVLVHKLTPKILELRSRV
                     ELGLAIELSVPLESVWSEIAQVDFVQIMSIATIGAQGHPFDALAFDRIREVRNKFPDM
                     IISVDGGVSVENYQILREMGVKRLVVGSHFNELWTYLTKRY"
     gene            17914..18777
                     /locus_tag="PLD99_00710"
     CDS             17914..18777
                     /locus_tag="PLD99_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRH25461.1"
                     /translation="MDISDEKILDIEKKANDIRISIIDMLLEAKSGHTAGPLGMVDIF
                     ATLYFHTLKHNPKKPDWSERDRLVLSNGHICPVLYATMAHAGYFDVKELKTLRKFGSR
                     LQGHPHRDFLPALETSSGPLGSGLSQAVGMAIADKMDHKDGERRIYCMLGDGELDEGQ
                     NWEAVMLAGKNRLNNLVAVVDRNNIQIDGFTEDIMPLESLSDKWRAFNWFVIEIDGHN
                     IEEIADAFEKAKAIYEKPTVIIAHTIPGKGVKEFERDYRWHGKPPNKDEAKMALQELR
                     TLGGKIKSEHE"
     gene            18779..19762
                     /locus_tag="PLD99_00715"
     CDS             18779..19762
                     /locus_tag="PLD99_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRH25462.1"
                     /translation="MLNSNLKLNKKIFDKDIEQAPIRKGFGEGLVSAGEENPQVVALC
                     ADLTESSQTHLFKEKFPNRFVEIGVAEQNLASVASGMAAMGKIPFITSYAMFSPGRNW
                     EQIRTTIAYNNRPVKIAGSHAGISVGPDGGTHQAVEDIAITRVIPRMVVISPCDTIEA
                     KKATIALAKTDTPVYLRLAREKTPIMTTIDTPFEIGKAQIVWESKNPKVGIIATGALL
                     YKALEAAKELEGKVEVEVMNLSTIKPLDEKAVIALAKKAGKIVTVEEHQVHGGMGGAV
                     AECLAINFPVQMKFIGVQDEFGQSGTPDELIEHYGMGKKAIIEAVKSLKQT"
     gene            19887..20441
                     /locus_tag="PLD99_00720"
     CDS             19887..20441
                     /locus_tag="PLD99_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25463.1"
                     /translation="MAKYPDVTAGQTEACINRMGGWHNFLRFIGGQGKIVFDPNPLLT
                     LLRTVEIPAQPKLTTSGDYFEEAGMKWMGDNFQAQFFGLEVSATEPSNIKVSKLEKDS
                     LDVEILANLGDEAEISIQEFKTFLATNRESSGVFIFYLRGNDGNLWAVRAGWSVISQS
                     WSVGASPATDTGRWDADHRLVSRN"
     gene            complement(20536..>21542)
                     /locus_tag="PLD99_00725"
     CDS             complement(20536..>21542)
                     /locus_tag="PLD99_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRH25464.1"
                     /translation="CIHGYNCIMHDFIAVGDIVKDTFIRLKEAEVKKVDEEHSTLCMA
                     FGEKIPFESATVIHAVGNSPNAAVSAARLGLSSALISNLGDDEDGKECIAALEAQGVD
                     TSLLTTHKGTTTNSHYVLWYKDDRTILVKHEEYNYKFPDFKAPKWLYLSSLGESTLDY
                     HKELNAYLVMNPSISLAFQPGTFQMKLGTTELKDIYKRTTIFFCNIPEAERILGVNTL
                     GVKELIKRMHALGPKIVVLTDGHNGTYAYAGEEVIFQPIYPDEKPPYERTGAGDAFAS
                     TTVGALALGKDLETALKWGSINSQSVVQQIGAQKGLLTRKEIEEKLKTAPLGFTSSTL
                     "
BASE COUNT         6169 a         4221 c         5419 g         5733 t
ORIGIN      
        1 tcaacctaaa caatgcaaat acttggactg cactccagac tttctcgaac gcttcaacca
       61 ccttactttc ttcaacaatt gcatctacat cgcagctgat gttgtctggt gcagcaactg
      121 gatgtcttac ttctactaat ggttggatta catccaccgg atctgcttgt ggtggggtag
      181 gtggatcatc cttctctttc ccatggacga cacttggaac aggggagaat gcaacctcaa
      241 ccattctttc tctacaagga gggttcctcg caagtgcatc atctacactt gcttcaacta
      301 cagttacaac attgttagca aatagtgcta caacaacaag cttgtttgta tcagaccttg
      361 cctcaactac cgcacttaga gccaacactg cgaatatagg taatcttact gtggaatcat
      421 gtactggatg tggaggagca ggtggctcgc tacagggttc ttacgatagt ggggcgacaa
      481 tagagaccgc atcgaatgtg gccgttgtta ttactgagac aagcgctgcg gccaatacgg
      541 gcgatctgtt acaactcaca gcaaatgcgg ctaccggagg tactttctct ggagatgcat
      601 tacagataac catggatgct gtggatgcga acggttatac aggaaatggt ttgcgcatag
      661 tagtagatca atctcaaaat actgggtcac ctatacttat agaagatgat gcgggagttg
      721 acttactagc agtttctgaa acaggaggat taactgtggg tagcgcggca aacggtagaa
      781 gtgacataaa tgtttatggc gatctcatta ctaagggcta taacgttaga aagtctttgg
      841 caaacattat tgatctcttt gtctacgata ctactcgtga ctcagatcgt ggtcattggc
      901 gcacaagctt aaatgttcaa ggtacctctt ggtacacaga gactaaggat gacggaccat
      961 cagatgcttg tgttattgct acggacgata ggtgtggatc gtctgaattc cctagaacgg
     1021 ctatcattgc tactacagcc gacggagtgt atatctttga tgctaaggat aactcattgt
     1081 ggatgaagtt cactcaagcg ggtacatacg cactcggtgc ggatactaac aataatccat
     1141 ctggtgtaag cgcggttaat ggtgttgttt atgtcggtac gaacggtgcc tcagcaacag
     1201 gtatgtatgc cttcgacttt gctcaggaca agatgtatag gtacaacgct acaaaccgtg
     1261 tagaggcaga tactactatt ggtacaagaa actcagctac atcatatgca ggtgatccaa
     1321 ttaccgcact cgcgatacta aatgccaccg taaacgacgt ctcggcagct cttatgaacg
     1381 ctggagcaga tgccttcgct aacgtggtga cactactaac ggatgccggc gctggaccgg
     1441 tgaagggttt agtaaagatt gctgttgcaa atgactcagg tgtgtctgtg gtgagcccag
     1501 tagagcaaaa agtgtttaac tactctgatg ttgcgaatga tgactacaac accgtgttcc
     1561 taacatcacg tggtcgtatg tatgctgcta atgagacaca agcacagcta gagagttgga
     1621 ggaatgttga tcgtgacaat acgagtgagg tggcgggtac gcctgacaag atttatgacg
     1681 aggttgtagg tagggcacct attacaaatg gtgttgcgcc taccatatta acttctcctg
     1741 ccacattggc agttgtagag agagcgtctg gtgctcttga gtcagctgct gctggagtag
     1801 tagatgttgg agacttggtg tactttggta ctaatcaggg attcgttgag atccaggaca
     1861 gcggtggttc gactgttggt gccaatgcag acggttggac taaaattact actacaggta
     1921 catctactcc ttggatgcac gggccagtga gatcagtttt cctattcgac gaagtgactg
     1981 gttctactga tgcagtgagt gccgttggag gtgctggtac aggagcaaat aatagattag
     2041 atgctgcgaa cgccacaaac ccaaccttca gcggtaacgg aggagtaagg ggtggaagcg
     2101 tcaacttcaa caacaactca tacttctgtt ctgatgcgaa caacgacggt acgtgtgaca
     2161 acgacacaga ctataacgtt gcagccatat cctttaccat atcactgtgg tttaagcact
     2221 cgactactgc tgcagcagat actttgtttg agaagtgttt tgtaccagca acaggtgttg
     2281 ctgccggagc ctgtgttgca gccggcatga atgcatctgg tcaaattacc tttggtatag
     2341 acgacgacac tacttgggca actggctcaa caacagttgc tatggacgac gcaataacaa
     2401 gtacaaatac tttcaatgat ggtagatggc atcacgcaat attcaccaac acagacaccg
     2461 acatttgtat gtatatagat ggtagacagg ctgttgcttg tgactctact ttggcggcta
     2521 cagcaacact cgacactggt tcagctacgc ttactattgg aggacagtgt agtgcggcgg
     2581 cttgcgcgac tggtgttgcc ttctgggatg gagaggtgga tgactttgta tggagttcta
     2641 acctcaacac tactattgga ggtatgaatg ctgaggctgc gagaaaacta tatctttcaa
     2701 gtgcccagga aagagaggtg aggatggctt ctactactga tgcaactacc ttctcctcaa
     2761 acactattgg tgactctggc gagagctgga gagtgaacga atttaccggt cgtatggtta
     2821 cgatagttga cggtactggt attggtcaga ctcgaagggt gatatcaaac actgctactg
     2881 tactcacagt ctttcctaat tggacagtaa cacctgatgc tacatctgac ttcaaggtag
     2941 atccatctag actttatggt tactccgaca acgtgacttc tgtcgcagtg gacttgccac
     3001 aggcacttaa cactcaaaga aatagtgttt atgtgggcac aagtaatgga tcggacggag
     3061 ggggtgtgac agtctttacg aataacttag ttacagacta ttatcatacc gacgcaggac
     3121 tttcgagtga tgacttcggc acagcatggt caggcactga tgcagatgat atacaagcaa
     3181 tagcagcata caatggcact atcgccattg gctccctggc aacaataaat gttgtgaagc
     3241 aagataaaag ctttgaacaa accagattgg agacaattgg tgcgctcaac ggtatccaga
     3301 ccgagcttat atccaaacac ctcttcggct cctcgatgga aagctttggg gtaggtgctg
     3361 gcgccgactt ggcagagtat tattcttcta ctcaaccgct agagaggggt gagatagtga
     3421 cccttgatcc aactaatcca cacgcagtgc tacgcgctac taagccttat cagagtgagg
     3481 ctctcggagt tattgctacc cagccgggta ttatcctcgg cccatatgta gacaccagct
     3541 tccctgtagc attggttggt agagtgccgg ttaaggtgac tactgagaat ggtgttatta
     3601 agtctggcga cagggtcacc tcggcgacac ttagtggtta tggtatgcgt gctactaaat
     3661 ctggcagagt gattggtgtt gcattagagg atgcgaagat tgacgacttc ttcgagtgtc
     3721 ctgatgaccc tcagggtgtt gcagagagaa ggtgtgggca ggtcatggtt tttgttaacc
     3781 tggttgatta caacggtgag tcaatagaca tgctcttaag cgagatagaa gaaggagtag
     3841 acttcgtctc tgctgacatc accgctacta ctaccgctac atcaacaaca agcactcttg
     3901 accaagaaac tgagttagac aaggatgtga gggtaataaa gttacttaag agtattaatg
     3961 ttaccggtca aatagatgct gagatactaa cagacagact ctctgctgcc tttgagattt
     4021 actctccaag aattattact gaaggtttga gagtcgatta tgtttcggcc ttattcgaag
     4081 aaatcggctt taagtcagat gtagtattct ttggcaggcc atactttact actgatactg
     4141 ccggatttgc attagtaaaa gcgggggata taagagtgga cgtcgtgttt gatagagagt
     4201 acttggagac accggttatc aacgcaagca tgtcgtctaa ggatggctta agttctgtta
     4261 accagtctgt tgctattcaa gctttctttg gtaaagatat taagttcctg ataactaatg
     4321 caaccactac aggctttacc atctacctta acaagccggt tgatgaggat gtgagcttca
     4381 actggatagc actagcagtg aagtcagcaa aaatgtttga gtcaagcggt agctccacac
     4441 ccgttaatgt agtagatgag gaccaaagtc cagtacagga agatgagcct gttgtgggtg
     4501 agactaatga tctgatggag gagtctaccc ctgttaatgt gggagatagc tcgacaacaa
     4561 cgcccgagat cttacctcta aatgaggtcc ccgtagcaac agaggattcc acaaccccac
     4621 cagtagaaga gttggcacct gaggaagttg ttgtagtgga tgtgcctgtg acagaagaga
     4681 gtataataga gttaccagta gcagcagatg agccacaaca ataagaaact tataatattg
     4741 gtaccggttg tatttgtact tgggttagta tttgggcatt taattaattt tggtgaaata
     4801 gtaaatgatt caagagagat aagaatggac gatggaggtc ttactaaccc cctacttgag
     4861 tgtgaagttg ctgaggacac tatcgcctct aggaaggtcg actttacccc taagttgaag
     4921 gattttatat cagatatgag tttgaggggt gttaataata tttctgtcta ttttagagac
     4981 ctaaacaatg gccctattgt cggagtcggt caagacaact catttgcacc ggcgagtttg
     5041 ctcaaggtgc cagtattaat agcatatcta aagtggtcag aggataaaac aggtcttcta
     5101 gacgaagtgg ttttgtttga agagagtaag ggtattgatt atgaacaagt ttttccgcct
     5161 gaggagaata tagttgttgg tgagtcgtat acagtaaggg agcttctcga aagaatgatt
     5221 aaatactctg acaatcaagc tctactactt cttcataaaa gaatacctat taagtaccaa
     5281 gaggagttgt atacattgtt gggagtagat aagagtataa taactgatcc aagtggtagg
     5341 ataactgtta ggcagtatag tattttcttt agaatattat ttaactcctc tttcttatct
     5401 agaagtaatt cagaatatgc cttgaagtta ttgtcgagta tagcttttga tgaaggatta
     5461 agagccggtg taccagataa agttttggtc tcgcacaaat ttggagagag gcagacgagt
     5521 gctaatgagc agcagataca tgactgcggt attgtgtatt accccaatca tccatatctt
     5581 ttgtgcgtaa tgactagagg tgaggatgtt aagagtctgg tggaaggtat caagaacgtt
     5641 tccgctttcg tttttggcat aattgacgag cagtataggg caatttaata ttttttttgc
     5701 ttagcggggc atgatattat agtaatatgt ctagagtgat acatatcatt aagaataata
     5761 ttgttcttgt tcttattttg ttgcttgtgg tgatgactgg cgcatcaatc tatttgtaca
     5821 acaagagcgc taggttaagt gcagacccga atgttaaggc tgaagaggag gtagcaaggt
     5881 tggttgaaag ggttggtaag ttgatctatc ttccagagga cgagacgcct actatggcaa
     5941 cagtaagtga cccagagttg ctcaaagatc aaccgttttt tgctaaggca agagttgggg
     6001 ataaagttct cttataccag aagtcgaaaa aagcctatct ctatgatcca aagagtaaca
     6061 agctcttaga agtagcacct attaatatag gaacagagga gaaataaata gaaaatgaaa
     6121 attaagtatc ttcacttaac tttatttata cttacattta tttttgtcgg tagggtagga
     6181 gcgcaggagt ttacctctac aaactttaag attttagatc ctgtggttaa ccctggggag
     6241 tactcgtctt ctgcaagctt caccttaagt agtgtgctta cccaattagg tattgggaca
     6301 tcgagtgaga gtacttacaa cttatttggt gggttccttt attttccatt cattacaacc
     6361 cccactctct cagccactcc tagtagcggg caggtgtcac tgtcgtggac agctgccgac
     6421 ggagtacttg gctggtcagc aggatcatac tctgttggtg agggtacagt gtctgggggg
     6481 ccgtacactt atacaaatgt tggaaacact ctaagtacta ttagatcagg tctaactaac
     6541 ggaacgtctt attatttcgt aattagggtt ttggatacct tgggcaatgt tattgctacc
     6601 tcaacgcagg taagttctac cccagtcgct ggtggaggag gtggtggagg aggaggtgga
     6661 ggaggtggtg gaggaggtgg gagtgcgggg gtaagattct caggcagagc atacccacta
     6721 agtagagtta gtattcttaa ggatggtcag attgctgtaa caaccattgc cggtccgaac
     6781 agcaactttg ctgctacact atctgactta gcctcgggca attacacctt ctcggtaaga
     6841 ggtgaggata aaaataatct tgtttctaat ccgtttgttt ttccaatatt tattactgaa
     6901 aatgtgatta cagaagtggg tgggatattt atctcaccaa ccattaccac tgataagttg
     6961 caggtaaaga agggcgacaa tattgtgatc tttggtcaaa ccgcaaacaa cgctgatgtg
     7021 cttattagta ttaattctga acctgaacac tttgttacaa ggaaatcaga taaggatggt
     7081 atatatcttc tcaattttga tagctcaata ttagcgcttg gtgatcacac gacaaaatcc
     7141 aaagcgagca ccgaggatga aataagcccc tacagcagct cggtgtcctt taaggtgggg
     7201 aatgaaaata tcctttcagg acctgtaacc tgtcagatga aggccgacct aaacaatgac
     7261 tgtcgtgtta atcttgtgga cttttccatc gcggcatttt ggtataagaa aagtttgagt
     7321 actaccttta aggaagtaga aaggacaaaa ttgaatgcag acaataagat agatttggtc
     7381 gacttcagta taatggcatt tcattggaca ggataaatat gaagaaactt aaagtattaa
     7441 tagttagttt agtgcttttg gtcccagtaa ttacttttgg tgctgaggta tctttggtat
     7501 caaaagacaa agaatatgtt gttaataagg agttcacagt taatgtttat ttggacactg
     7561 aaggtgattc gataaatgca gttgagggtg acatttcatt taaaccaagt gttcttgagg
     7621 tcgtcgctat taaggacggt aactcctcag ttaatttttg gattaataaa ccgggtttag
     7681 ttacgaaaga taaaattagt ttctctggtg taactccagg aggagtgcgg ggtcaagata
     7741 ggctactttt ctctatagtt tttaaaccaa ttaagagtgg tgagtctaat gtagatattg
     7801 gcgatgttgt gttactcaaa aatgacggac tcggtaccaa ggcttcaact aaggttgttg
     7861 gtttgtctat aaaagttgtt ccaagcacag ggggtccagc tacaaacgag gtggttatag
     7921 atgatcgaga gccagagact ttcttgcctg tgataggtcg taatagtgag atatatgagg
     7981 gaaagagctt tctagtcttt agtactcagg ataaggagtc tggtattgaa tattatgagg
     8041 tgaaggaggg tatcttcggc gaatatacca gagcgataag cccatttgtg ctgaataacc
     8101 aaaaattaga caaaaagatc tatgttaagg cggtagatct aaatggtaat actagagtcg
     8161 tgtctataaa gccagagaac tacaaagttt ggtataaacg atctgaaatc ttaggtataa
     8221 ttatcttagt aatactcgta gttttttata atagaaaaaa atggttcaat cctgcagaaa
     8281 agttgtagca ctagtaacgg tgcttctcct cgcaccagtt tttgttagtg cagcaacgct
     8341 aagtattagc ccacagagtg gaagttatga ggtgggggat cgatttactg ttagggtggt
     8401 aatgtcggct agtgaggcta taaatgctgt gtccggcgag ttgaaatttc ctacatcggt
     8461 ctttgcaatt gactctgtat cgaagtttag ctcgatactt aacttttggg tctcagaacc
     8521 gtcatactcg aataccttag gaaaagttaa tttcgaagga gtggttcttg gtgggttcca
     8581 gggtggtact ggtactgtag taactcttaa ccttagggct gttagcgtgg ggagcgggag
     8641 aatcgatttt gtttctggcc aggttctggc taatgatgga cagggtacga atgttaccca
     8701 aggtacaacg ggagcaaatt tttctatagt tgaggcaact aaaccaaagc ctgtagagaa
     8761 gaaagttgag gttgtagaag aggttgctaa acctgattta gcagcaccag agattgagtt
     8821 ggtcagtaaa tatggagaaa gggctgtctc aggaatatcg agctatccaa aatcagaggt
     8881 agtacttacc ttcgtaaacg aggtaggggt aaagatattt atacttggct ctacagatga
     8941 tagtggtaag ttcttgcttc ttgtgcctaa gtctctaaag agaggcgagt actcggtgtc
     9001 agctcaggtt gtacaaaagg ggtcgtccta tagtgctgta tcaaatacga ttaaaataaa
     9061 aataggtaat atcatttctg atattggctg gggggcgagg attgccttta ttctcctcat
     9121 tcttgctttg atatatgtgt cgtacagact aagaatgtat tttgttaaaa actctaaaat
     9181 gaagtatgag gccggtgagg ttaaggacgt ggtgaagaag tcctttaaaa tcctagaaga
     9241 ggatatagaa aattccaagg acaatgcaaa gaatgctaaa gacagagagg gtttgagcaa
     9301 tactcttaag gatttaagcg aagcagagag tgtgatacta aaagagataa aggatataga
     9361 aaagttatga tcaaggtaag caaatggagc tcactacttg tcctggcctt attactctct
     9421 tggtcgaatg tgcgagctca gagtataagt gttgatgcct caagattatt aagaaaaccg
     9481 gagatcatcc tctctccaag gacaggtagt tttgtcgaag ggtcaacctt tgatgtacca
     9541 atacttctaa atacaaatgg cacgagtatt aatagtttgg aagttagact aaatttcgat
     9601 aggaataagt tagagatcgt gagaccaaca ggcggggagt ctattattgg agtgtgggtc
     9661 gagccaccga agtttgataa taagaacggt accgctagtt atgtgggagt cattacaaac
     9721 ggtattacca caaattccgg acttattggt acagtgactt tcagagccaa aagtgcgggg
     9781 agagcgactg tttatattgg ttctgattct aaggtgctta tgaatgacgg attggggact
     9841 gaggttgagt ctgagtttgg tcgtgcggat tacagtatct tggtaagacc acctgagggg
     9901 gtgagagtct tctcggagac gcatcagttc caaggtcagt ggtataacaa taataatcct
     9961 agcttgtctt gggacaagga tgttggggtg gatggattta gctatatttt ggacaataaa
    10021 ccatctacta ttccggagaa taaaattgta actaccgaaa ccacaacttc gtatgagaat
    10081 ctggctgacg gactttggta ctttcatatc aaagcgagga agaacggggc gtggagtgcg
    10141 acaggacact tcttagtaag gatagacact aaaccaccag cactcttcaa accagaagtg
    10201 gcttatctta ctaaagacaa aattgcggac aggactctta tctccttcgt gaccactgac
    10261 aacctctctg gtgttgatag atatgaagtc ggtgtgatcg acaaagctga ttcttcgaat
    10321 acctctccac tctttgttga ggtggagagt ccttatcagg tacccatagt tggttcaggt
    10381 tatttgaatg ttattgtgag agcaattgat ggtgcgggta atgtggttga tgtgagttta
    10441 gacattgagc cacagacttt tgcttcgagc tttatcaaga gtaactggtt atcagtactc
    10501 ctaataattc ttgctgtact tataaccgga ttcatcacta actactttgt tggacatcat
    10561 gtgcttagac atttcagaag ggcggttgaa tttatcaaac gagacactgc gacaagtgac
    10621 gcagtgcttt cgcctcctcc acccccacca ctatttcctc ctataaatga taatattagt
    10681 aaaaacggca atgaaccaaa taaataataa tctaacagag gaggagaaac gggtgatgtt
    10741 cgagaagggg accgaagcgc catttaccgg taagtacctc cagtctaaag agagtggggt
    10801 atatgtgtgt aagaactgtg gcacagagct tttcacatca gatgctaaat ttgactcagg
    10861 aactggctgg ccatccttcg acaatccagc taatactaag aatgtcatct tagaagagga
    10921 caatagctac aatatgcatc gcacagaagt aagatgtgca aagtgtggag cccacttagg
    10981 gcatgtgttt gacgacggtc caacaggcac agggaagagg tactgtatca actcagtttg
    11041 tcttgacttg caaaaataag agtagtgtgc tataataact ggtgatgtaa gtttctcgta
    11101 agtttccggg ctgtgcctag aacgtaaaga gaatcgctgg cgcttacatg tttaatattc
    11161 aggccagcat taaagtagat gtcaacaaag ctttacgtcg gaggtattcc ttacacttcg
    11221 actgaacaga accttaagga cgctttcggc aaggccggaa acgttctctc agcttcgatc
    11281 atcattgatc gcatgactgg tagatctaag ggattcggtt tcgtcgaaat gtctaatgat
    11341 gatgaggcta acgccgcaat caacatgtgg aacggccagg attttgatgg tcgcaagctc
    11401 acagtcaatg tggctcgccc attagagcct cgcgctccag gcatgaatcg ccgcccaatg
    11461 taatattggc gaaagccaat gttcgttcga aaaccccgct tcggcggggt ttttgtttta
    11521 tactactggt atgtataaga aagtcttgtt gctcgtcgtc ctctcgctac ttttgtttat
    11581 tagtctaaag tacctaaacc ctgggtgtga ctgcaatgtt acagagaggg taacgatagg
    11641 tggtactgaa atgcaggcct ttgtgagtga cacagaagac gagagatcgc agggcttgtc
    11701 cggcaaggaa gggctagggg agggtgaggg aatgctcttc gtcttcgaat acccggctaa
    11761 gtacggcttc tggatgaagg atatgaattt ccccatcgat attatctgga ttggtgcaga
    11821 aaagagggtt ttagctatag aaaaagggtt agaacccagc acatatccaa aggtttttta
    11881 cccaccagac aaggttctct atgttttaga ggtgccatct ggtttttccg acacagcgaa
    11941 aattgaagtg ggtcaatctc tctctatcgg acaataattt tcatcaggga tagggtcttt
    12001 tcacacctta ttttaaagct tttttgggag ggatctttac acatcccaga gaatatttgt
    12061 agtaagaaaa gaaaaagtta tccacaggat ttatcatgat tgtttacata aaattactct
    12121 aaataatcga gtaatttata tggtcatata tgtaacaaga cgagatggta ccaaggagcc
    12181 ctggaacgcc gattacatta acaagtcgat tgagcgagag actaaaggtt tggctgaccc
    12241 aattgccaag gttatccaaa tcgccaatga tactcgtctt actgtctatg acggcatcac
    12301 aacagacgag atggaccagg ccaccatcaa tgcagctgtg caaaacatca aggatgaccc
    12361 agccgagtac gacaagatcg ctactagact ccttctcaaa actatctaca agaaagtcgt
    12421 tggtcactac aattcggatg ataaagaaac tctcaaggtt agacatagag aagggtttat
    12481 aaagtttgtc attgaaggag tagagcaggg aagactccac ccaaatatga cagctaagtt
    12541 cgatctcgag cgcctctcgc atgctctcaa aattcagcga gatgacctct ttttgtactc
    12601 tggacttgat ggtctgctta accgttatgg tatgaaggac atcagccaaa ggcctcttga
    12661 gactcctcaa tacatcttta tgcgtattgc tatgggactc tcttataacg aggctgatcc
    12721 gaccgcatgg gctatccgct tctatgacaa gatgtcaaaa catgagtata ttgctggagg
    12781 ctcaactaat cttggagcag gaacggtgcg tgcagcactg tctaactgtt tcctccttga
    12841 gatccatgac aatatggagc acattgcaaa gtcagtttcg gacgtgatgc ttctctctaa
    12901 gggctcaggt ggaattggtg catctgtaac caaacttcgt gcagcaggtt cacctttgaa
    12961 gagtaacttc ggtggagttt ctacaggtcc aaccacattt gcgaagatta ttgatacagc
    13021 aatacgcgca atacagagag gagggaagaa gaagggggct ttagccttct acatggagaa
    13081 ctggcactta gacttcccag agtttttgga ttggaagcat aatgcaggtg acgattatct
    13141 tcgtatgagg accgcgaaca ctgctgcttt tatctctgat gagtttatga agagagttca
    13201 agcaggggat gagtggtatc tattcgatcc agctgagact cctgatttga atgagactta
    13261 tggcaaggtt ttctcaaagc gttatgccga gtatattgag atggcaaaca agggcgagat
    13321 tagagccttt aagaagatgc ctgcaagaga gttgtggagg aagaccttgg ttgctttgca
    13381 gaccaccgca catccatgga ttacttggaa ggatcctatt aatcttagag ctttaaataa
    13441 caatacaggc acgattcata tgtctaacct ctgtaccgag atatgtctcc ctcaggacaa
    13501 ggataatatt gcagtgtgta acctaacatc acttaacctg gctgctcatg tgtctgagaa
    13561 gcaggttaac tggcagaagc ttgaggagtc tgtgcgtcta gcagtaagac aactagacaa
    13621 tcttatcgat atcaacaagc tcccagtgcc agaagcggag aagtcagata aggagaatcg
    13681 tgctgtaggt ctaggagtca tgggtcttgc agatgtattt gaaaggcttg gtctcgctta
    13741 tgactctgag catgcatata actttaccga taggattttc gaattcgttt cttatatggc
    13801 gatagacgag agtgctaact tggcagccga gagaggtgtg tataagaact tcgaaggttc
    13861 tagatggtct aagggcatgg tgccatttga tacactagag gttctagaag agagaagggg
    13921 tgtgcctctc acagttgaca agaccagtca tcacagagga ctcgattgga acatcttaag
    13981 agagaaggtg aagaagggta tcaggaacgc aactctcatg gctgttgcac caaatgcaaa
    14041 cattggacta gtggtcggca cagtgcctgg tattgatgcc cgcttctcac aagtattctc
    14101 aaggaacaag atctctggta agtacctaga catcaatcac aaccttgtga gagatctaaa
    14161 gaacatggac ctttgggaga aagtgaaggg tatgattatc gagctccaag gagacatctc
    14221 atctatccct gagattccag accacattaa agcgatttat aaaacttctt tcacaacctc
    14281 tccatatgct tatatagaag tcgcggcccg cgcgcagaag tgggttgacc aagccctgtc
    14341 tcgaaacatg tatctcgaga caagagatat cgacgagact atgcacttgt acgaaacggc
    14401 gtgggagaaa ggggtcaaga ccacttatta tctccacatg aagcctcgcc ataccgcgga
    14461 gcagtccact gtggcagtca ataaggctgt aactatgggt aagagagggt ttgccgggct
    14521 aagttcgagt ccgaaggtag aggtagaagt ggaggggtcc aaggtgccag tatggaatca
    14581 gaaggaaata caaccaaagc tcatagacgc aacaccagta agaaagattg agcaacctgt
    14641 tcattcagga caggtagtcc gagtagaaaa cagagagacc gagaaagtag aggtaagacc
    14701 agtttctcag acatctaagc tcactgtgca tttaccagat gatccagcga acgcaaatat
    14761 ctgcgacgac tgtcaataat aataaattaa ctaatatgct agtaggaaaa gcgtccccag
    14821 acgacatgaa tttgcatccc atacgctatc aatgggcgta tgacttatat aaccaagctg
    14881 ttagaaacac ctggttccct cacgaaattg cgctcaaaga ggacttggac gactgggcca
    14941 aaatgactga ggatgaaagg catgctgtga agttccttat ggcattcttc aatccagcgg
    15001 aacttattgt aaaccgctct atcgctttag gtatttaccc atatcttaag agtccagagt
    15061 gtcacctata ccttgctaag caaatgtggg aggaggcgaa ccactgcgtt gccttcgagt
    15121 atgtgctaga aactttcccc ttcgacagag agaacatctt caacctccac ctctcagtgc
    15181 cttcgatgac agccaaggag gcctacatca acaagtatat gaagaggatg accgaggata
    15241 gtctagatat tgacacaatg gatggtaaga aggacttcat aaggaatctt gtggctacaa
    15301 acatcgtgat ggagggtgtg tggttctact ctggctttat ggtagctctc tcatttagac
    15361 agcgtaacca gcttcgtaac ttcggctcga tgatcaactg ggtactacga gacgagtcac
    15421 ttcatctaaa gttcggaatg aacttggtcc acaatattct tgaggagaat ccagaattgc
    15481 tcactgagga ttttgctaat gagattcgag acattgtggt tgagggagtc aacttagaga
    15541 ctgcatacaa caaagacctc ttcccacaag gaatcttggg actcaatgct gactatgtga
    15601 atcagtatgt acagtatgtg gctgataggc gtatggagga gttaggcctt ccaaagtact
    15661 acaacgctac gaatccagcc aagtggatgt ctacagcgac cgacgtgtat gaactcgtaa
    15721 acttctttga ggcgcagaat acttcttatg aggtggacgg ccatagtgct actgttaaga
    15781 aatctggttc atctggtcaa tcagaggagc ctaggaaggc cccagatgcc ccaacaatac
    15841 ctaccggagt gggggaataa gcagtggtac aataatagat atgaaaataa gagtagcaat
    15901 caacggagcc ggccgtatcg gcagagcgtt ttataagctt gcaagagaga acaagaagct
    15961 cgaggtggta gcaatgaatg atttgggtga tattgcaaac attgcgtatc ttcttaagta
    16021 cgataccacc tacggacaga gcggactaaa gatcgaggtg aagccagaca actcagccct
    16081 tatcattgat ggggaggagg ttaagttcgt acaagtgcgt gaggccaagg acttgccctg
    16141 gagagacatg gatattgatg tagtggtgga atctaccggt ctttatacca gttatgagaa
    16201 agctaaggca catctagacg caggcgctaa gcgtgtcgtg gttaccgctc ctatgaagga
    16261 cgacccaata ccaggtattg aaggcgccac agtgctcatg ggtattaacg atgataagct
    16321 tgctacttgc cagatcacct cgaatgcatc gtgtactaca aacgccgcct caccagtact
    16381 agccattctc gacgaggcgc taggtgtaga gaaggcgata cttaacacag tgcattcata
    16441 tacagcatca caatcaatcg ttgatggtcc taacaagaag gactggagag agggtagggc
    16501 agcggcgcaa aacatcgtgc cctcgtcaac cggtgcagca attgctgtag gtaaggcact
    16561 cactcaactt gatggtaagt ttgatggtat atctatccgc gtacctacag ttgtggggtc
    16621 tattgcagac attaccttca ttgccaagcg agacactaca gtagaagagg taaacaagat
    16681 tatgaccgag gcagcaaaga cagatagatg gaaggatata ttcgaagtga ctgacgagga
    16741 aattgtttct tccgacatca agaatgcagc acatgcttct attgttgatc ttgctatgac
    16801 tagagttgtg ggaggtaacc tagtaaaggt cctggcttgg tacgacaacg agatggggta
    16861 cactcacaca ttagtgcaac atgtggttaa gtcagggtct caaataaaat aatgaaaata
    16921 tttgttggat cagatcatgc ggggttcggg ctcaaacaga agatgattcc gtttcttcaa
    16981 agtcttggtt atgaagtcat agacaagggc gctactgagt atgacgaggc agacgacttt
    17041 ccagacttca tctctcaagt agcaagagag atttcgatga accccaacaa cgctaagggt
    17101 attgtcatgg gaggctcagg acagggtgag gcaatgcttg ccaacagatt caaaaatgtt
    17161 cgggcgactg tctattatgg aggtggtaag tcattggtcg aggacaatga ctctattatt
    17221 aagctctcgc gtgatcacaa tgatgcgaat attctctcta ttggcgcaag gtttgtcact
    17281 gatgacgatg ctatgagagc tactcatgag tggctagaaa cttctttctc ggaggatgaa
    17341 agacataaaa ggcgaattgc taaattcgat agagttacac atgagtgagg tcctaccagc
    17401 aatacttcca gatagtgctc aggacttggt cgacaaggta gcgctcttac ctaaagaaat
    17461 agagatacta cacttggatg taatggagag tgatgtgtgg gctccaatag gcaaggactt
    17521 cgaagcacat ttgatggttg agtacccaga ggatcttata gagagatgga tagatagagg
    17581 tgctaagagg gtcttggtac acaagcttac tccaaagatt ttagaactcc gaagtagagt
    17641 agaacttggt ctcgctattg agcttagtgt gccacttgag tcagtgtgga gtgagatcgc
    17701 tcaagtggac ttcgtgcaaa taatgtctat agcgaccatt ggcgcacaag gtcacccatt
    17761 tgatgcccta gcatttgata gaataagaga agtgagaaac aagttccccg atatgataat
    17821 aagtgtagat ggtggagtga gtgtagagaa ttaccaaatt ttgcgagaga tgggtgtaaa
    17881 gaggttggtg gtggggtctc actttaacga attatggaca tatctgacga aaagatacta
    17941 gacatagaga agaaggcgaa tgacattcgt atttccatta ttgacatgct cctcgaggct
    18001 aagagtgggc acacagctgg accacttggt atggtagata tttttgccac tctatatttt
    18061 cacactctaa aacataaccc gaagaaaccg gactggtcag aaagagacag gctagtactt
    18121 agtaatgggc atatttgccc tgtgctctac gcgaccatgg ctcatgcagg gtactttgat
    18181 gttaaagaat taaaaacact tcgcaagttt ggctcgcgct tacaaggcca tccacacaga
    18241 gacttccttc cagcgctcga aactagttcg ggcccacttg gctctggact ctcacaagcg
    18301 gtgggtatgg ctatagcaga caaaatggat cacaaggacg gagagaggag aatatactgc
    18361 atgctcggtg atggtgagtt agatgagggg cagaactggg aagccgtgat gctcgcaggt
    18421 aaaaatagac taaacaacct tgtagctgtg gtagatagga acaacataca gatagacgga
    18481 tttaccgagg acatcatgcc gcttgagtct ctctctgata aatggagagc ctttaattgg
    18541 tttgtgatag agattgatgg ccacaatata gaagagattg cggatgcgtt tgagaaagct
    18601 aaggctatat atgagaagcc aacagttatc attgcccaca ctatccccgg caagggggtc
    18661 aaagagtttg agcgcgacta tcgttggcac ggcaaaccac caaacaaaga cgaagccaaa
    18721 atggctctcc aagaacttcg tacattgggt gggaagatta agtcagagca cgagtaatat
    18781 gttgaactcg aatttaaaac ttaataagaa gatcttcgac aaggacatcg agcaagcgcc
    18841 gatacgcaag ggttttggag aggggctggt gagtgcgggg gaggagaacc cacaagttgt
    18901 ggcactctgt gccgacctca cagagtcctc tcaaacacat ttgtttaagg agaagttccc
    18961 caacaggttt gtcgagatcg gtgttgccga gcagaatttg gctagtgtag cgagtggtat
    19021 ggctgctatg ggcaagatcc cattcatcac ctcgtatgca atgtttagtc ctgggaggaa
    19081 ttgggagcag atccgcacta ctattgccta caacaacaga ccggtgaaga tcgccggctc
    19141 acatgcaggt atatcagtag gaccagacgg aggtacacac caagcagtag aagacattgc
    19201 gattacccga gtaataccaa gaatggtggt gatctcaccg tgcgacacaa tagaggcgaa
    19261 gaaggcgacc atagcactcg cgaagacaga cacaccagtg tatctccgtt tggcgagaga
    19321 gaagactcca atcatgacta ctatcgatac tcctttcgaa atcggtaagg cacagattgt
    19381 ctgggagtcg aaaaatccta aagttggaat cattgctaca ggagcccttt tatacaaagc
    19441 acttgaagca gctaaagaac tagagggtaa ggttgaggta gaggtaatga atctctctac
    19501 tattaaacct cttgatgaga aggctgttat agctttagct aaaaaggcag ggaagatcgt
    19561 gacagtagag gagcatcagg tacatggtgg tatgggtggt gcggttgcag agtgtttggc
    19621 aataaacttc cctgtacaaa tgaaatttat tggtgtccaa gatgaattcg gccagtctgg
    19681 aaccccagat gagctaatag agcactacgg catgggcaaa aaagccataa tcgaagctgt
    19741 gaaatccctc aagcagactt gacaaaacac taattttgta tatataattt agggtgatag
    19801 cacttttaca gcgaaggaga tcctgtccta atccctcaac ccacgggcag tgggaaactc
    19861 cgggctttca gctcaaggag atggtcatgg cgaagtaccc cgatgtgact gctggtcaga
    19921 cggaagcctg catcaatcgc atgggtgggt ggcataactt tctcaggttc attggcggtc
    19981 aggggaagat cgttttcgac cccaacccac tcctcaccct cctccgcaca gttgagatcc
    20041 cagcccagcc caagctcaca acctcggggg attacttcga ggaagcaggg atgaagtgga
    20101 tgggagacaa cttccaagcc cagttctttg gcctcgaagt ctctgccacg gaaccctcca
    20161 acatcaaggt gtcgaagctt gagaaggact cactcgatgt cgagatcctt gccaatcttg
    20221 gtgatgaggc cgagatctcg atccaggagt tcaagacctt cctcgccaca aaccgcgaga
    20281 gctcgggggt cttcattttc tacctccggg gcaatgacgg caatctgtgg gccgtgcgtg
    20341 cgggctggag cgtcatcagt caaagttgga gcgtcggtgc cagcccggcg acggatacgg
    20401 gcaggtggga cgctgaccac cggctggtct ctcgcaactg accctctgac tccaatccga
    20461 cgaaaggagg attggatggc cctcttcgta tgagggttca agagcgtttc gactctgtcg
    20521 agacgctctt tctttttata aagtcgatga agtgaatccg aggggagcgg ttttgagttt
    20581 ctcctctatc tctttgcgag tgagtaatcc tttttgtgcg ccgatttgtt gtacgactga
    20641 ttgagagttt attgaacccc acttaagagc tgtctctaag tctttaccaa gtgccaaggc
    20701 acctacggta gtggaggcga aggcatctcc tgcaccggta cgctcgtaag gaggcttctc
    20761 atctggataa ataggttgga agatgacttc ctctccagca taagcataag taccgttgtg
    20821 tccgtcagtg agtacaacta tctttggccc tagggcatgc attctcttga taagttcttt
    20881 aactcccagt gtatttactc caagtatcct ctctgcttct gggatgttgc agaagaagat
    20941 ggttgtcctt ttatatatat ccttaagctc tgtagtacca agcttcattt ggaaggtgcc
    21001 gggttggaat gcgagtgaga tagaggggtt catcaccaag taggcgttta gctccttgtg
    21061 gtagtcgagt gtgctctcac cgagggagga gaggtagagc cacttaggag ctttgaaatc
    21121 agggaatttg taattgtact cctcatgttt aaccaagatg gtgcggtcgt ccttgtacca
    21181 gaggacataa tggctgttcg tagtggtacc cttgtgggtg gtaaggagac tcgtgtccac
    21241 tccttgtgcc tcgagtgcag caatacactc tttgccatcc tcatcgtctc ctaagttaga
    21301 gataagagca gaggagagac caagtcgagc ggctgataca gcggcgtttg ggctgttgcc
    21361 cacagcgtgg attactgtgg cgctttcaaa agggatcttc tccccgaagg ccatacagag
    21421 agtactgtgc tcctcatcaa ccttcttgac ctcagcctcc tttagacgga taaacgtgtc
    21481 cttcacaatg tctcccacag cgataaaatc atgcatgata caattatacc catgaataca
    21541 ga
//
LOCUS       DAORIT010000005        21126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_396_length_21126_cov_50.949219, whole genome shotgun sequence.
ACCESSION   DAORIT010000005 DAORIT010000000
VERSION     DAORIT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21126
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_396_length_21126_cov_50.949219"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..512
                     /locus_tag="PLD99_00730"
     CDS             81..512
                     /locus_tag="PLD99_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HRH25465.1"
                     /translation="MRFQVPQYIEVEDKIFGPLTFKQFIYLAGSIGIAVVLLTLLPRF
                     IAIILGVPVVVFGVALAFFKVNERPFTLVIEDFIKFYIGSRLYIWKKEANKPSDAHNS
                     IEPGKSLEQLSVPKLSGSKLKDLTFELDVKQQSNPVTKDRL"
     gene            520..1158
                     /locus_tag="PLD99_00735"
     CDS             520..1158
                     /locus_tag="PLD99_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25466.1"
                     /translation="MASGALPTQSFVPIKEVRDGVIVEKDGGMRAILLASSLNFSLKS
                     VDEKNAILLQFQDFLNSLDFSVQIVVQSRKLDIRPYLALLEEQSKLQTNNLMKLQTRE
                     YIEFVRSFTESTNIMTKNFFIVVPYNPAIVSTGKSGGLSSLVSLRPKDKAGAKEASFE
                     EARTQLEERLAVVEQGLIRTGIRIARLGTDEVIELFYKAFNPGETEKATIKR"
     gene            1163..2995
                     /locus_tag="PLD99_00740"
     CDS             1163..2995
                     /locus_tag="PLD99_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRH25467.1"
                     /translation="MGFFDTLIGKKEEKSENRIQSILPQEVFEAGVLELKDLIAPSAL
                     KVTPRELDLGEKILRSFFVISYPRFLSEEWFSPILNMDKVFDVSIFVHPIETAKILRQ
                     FQKKVAEVESQIHSREEKGLVRDPVLDVAYQDLENLRDQLQQAQEKLFDVGLYITIYA
                     DSDLELDKIENEIKSILEAKLVYVKPALFQQEQGFKSTLPLGDDLLGVHSKLNSSPLS
                     SLFPFTSFDLTSDKGILYGINRHNSSLVLFDRFSLENYNSVIFAKSGAGKSYCTKLEI
                     LRTLMFDTEVIIIDPEKEYEYMAEAVGGRYFNISLNSEHHINPFDLPVPTADESAANI
                     LRSNIINLVGLFRIMMGGLTSEEDALIDRAITETYALKDITADSDFRNVEPPLMSDFE
                     LVLSGLEGGESLAQRLTKYTKGTWAGFINRPTNVDINKKLVVFSLRDMEDELKPVAMY
                     IVTHFIWNSVRRELKKRLLVIDEAWWMMKSDDTASFLTGLVKRGRKYYLGVATITQDV
                     GDFLKSPYGLPIITNSSIQVLLKQSPTNIDNLQKTFNLTDEEKFLLLESDVGEGIFFA
                     GLKHVAIKIIASYTEDQIITSDPSQVLAQKKARRELQETKEVNV"
     gene            2988..3332
                     /locus_tag="PLD99_00745"
     CDS             2988..3332
                     /locus_tag="PLD99_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25468.1"
                     /translation="MSKELSNGTILLMVSTAIFFDVLQIAFALIAMDWVVGIFGFLTF
                     FIWFKINGVSFLTPKRAITMSGSAIIEAFPFLSSLPTWTLAVIIIAMDTKAKQIAKKT
                     IGNTEDNKENKA"
     gene            3354..4061
                     /locus_tag="PLD99_00750"
     CDS             3354..4061
                     /locus_tag="PLD99_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25469.1"
                     /translation="MKKLFLLLVLGILIPICGTRAEGEVDLIWEASTFTHPFYEGLPL
                     WSNESSITLTAIPHLPNISPNNIIYRWSKNKTVLGSLSGIGKNSLSIKDTVLSLPVEV
                     TIDLFLENGSSPIGTATLSLTPTNPKLLIFENSPLYGMLSNKSIQNEFTIKEDEVTLS
                     AVPLFSTVSKRTAGAFEYTWLTNSGERRIGDSVTYRAPEGGSGSASVVVRANNSKIIA
                     QPKNVNFLIKFNEQNDF"
     gene            4042..>4349
                     /locus_tag="PLD99_00755"
     CDS             4042..>4349
                     /locus_tag="PLD99_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25470.1"
                     /translation="MNKMTFKKLFIFGILGLIFFLPTITLAQNTGVTGYTPLAPIPEL
                     MNKAGNQVDINSFIPGAIKLAISIASALSVIYIIVGGVQYITTDSMGGKSNAKGTIEN
                     A"
     assembly_gap    4350..4449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4450..5255
                     /locus_tag="PLD99_00760"
     CDS             <4450..5255
                     /locus_tag="PLD99_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25471.1"
                     /translation="TTDSMGGKSNAKGTIENAIMGLLLAIGSYAILYTINPKLVVFDI
                     KLSELEGVGIDFDGARNGPIVTPTTEDISKSLEGCNDCAQIPSGVRYKSAQANGCAMP
                     GPCVVNREVGQKLLTLTNSLSRSGISFQVTESWPPTRPHVASCQNPNNPDSGTCVDAN
                     FINAINLASPSDRTKVESFILAAQAAGLRAVYEVTNIGDRDRLISGNLDSGDVIAPRM
                     NVCVSGETTALNASTNRYSPCCPTKELKNNPGNITCSWISANHFSVYNK"
     gene            5258..6490
                     /locus_tag="PLD99_00765"
     CDS             5258..6490
                     /locus_tag="PLD99_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25472.1"
                     /translation="MSKKLTIFIIILTILLGSGILYYLLGGKGPSGLGGIPGLPFGEA
                     PGDDVGGTNNSDPNDTPNDGETPIDTVGGGTDGAKLTKITSEPVAGAITFINKGSTYL
                     RYVDRATGHVSEVKLSTMERTKILNITRPKIYEALWKKDGTGFIERTLDGEENIVNTS
                     VSLIQSTSTSSTTEPYIIRSTLLKGEVGEIKPGFDGGLVYNLTDSGVVATSNFIGEKP
                     KTLFTLPFTSWRIMPVSTTTALLTTKPSAISQGYSYLTNLSTGKLTKILGPLSGLSAK
                     IKSDSSRVIYSYIDNNKIELKAKDLKNGVVTDILPKTLADKCVWSKKAGAVLYCGAPK
                     SLPQGLPDSWYQGEVGFADSFWRFNTETNTAEELYVPQAESEGVIDSHSIELSPDEDY
                     LIFVNKSDLSLWVLKLPL"
     gene            complement(6474..7196)
                     /locus_tag="PLD99_00770"
     CDS             complement(6474..7196)
                     /locus_tag="PLD99_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRH25473.1"
                     /translation="MSALYNNLLVEPIYNTFVAIFNLFPSFGAGLAIICITLIVRLAL
                     FPISRKAVRAQVEMQNLAPELESIKAKYKDNQEEQARRTLALYKERGVNPFSGILVLL
                     IQLPVIFALYHIFISTGFPEIRADLLYSFVSVPETVSPFFLGADLTAKSVVLALLAAI
                     STFFQIKFASPVKPKVKSGAASFGDDLAKSMQTQMKYFFPVMVFFIAYTISGVVALYW
                     LTTNIFTIAQEIFVRNKLKAEA"
     gene            complement(7206..7535)
                     /gene="rnpA"
                     /locus_tag="PLD99_00775"
     CDS             complement(7206..7535)
                     /gene="rnpA"
                     /locus_tag="PLD99_00775"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRH25474.1"
                     /translation="MLSKQNRLERKDFTNLLTSKRFVNSENFSLRVGSGESKPKLAVS
                     VSKKVSKLAVDRNTLRRRVYSIVRDSVKELEPNTYLFVARPSAKKLKGEKLEKEITSL
                     LNQFKKR"
     gene            complement(7535..7669)
                     /gene="rpmH"
                     /locus_tag="PLD99_00780"
     CDS             complement(7535..7669)
                     /gene="rpmH"
                     /locus_tag="PLD99_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRH25475.1"
                     /translation="MSTTYQPKKRKRARAHGWMKRKATAGGRRTVTARRRKGRARLTA
                     "
     gene            7779..9119
                     /gene="dnaA"
                     /locus_tag="PLD99_00785"
     CDS             7779..9119
                     /gene="dnaA"
                     /locus_tag="PLD99_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRH25476.1"
                     /translation="MDQRQLWESILAEIELSVSKPAFATWFKDTHIVKRDENTIILSV
                     PNAFARDMFQSRYHATVLKLLRDKDHTIHAVEYVINRDEPKRRDEKIINTQNGNLPLP
                     ETLVNKDDNLNPRYTFENFIIGPFNELAHAAAQAVIKSPGLMYNPLFIYGSTGHGKTH
                     LIQSIGNHIKTVSPGKRVFYMTSEKFGQDCMNALQTQKMAVFKEKYRKYDVLIMDDIQ
                     FFSEKQKFQEELFHLFNTLYDNNKQVIFSSDKHPHFIQGLEDRLRSRMSAGMIVDIPQ
                     PDHESRIAILNAKSKLQNISLSPDVIDYLAKTVVGNIRDLEGALNTLIIHSQLKGREV
                     AVADVRNLIKSNQKPKRAVAVKDIVKVISDFYSVEEGSIYEKTRRKEVIKPRQIIMYI
                     LREDFNISYPSIGEKLGGRDHTTVIHSCEKIKEDLKNDQSLGQELSQIRNLLSV"
     gene            9323..10423
                     /gene="dnaN"
                     /locus_tag="PLD99_00790"
     CDS             9323..10423
                     /gene="dnaN"
                     /locus_tag="PLD99_00790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRH25477.1"
                     /translation="MKIESIKEKLVEAVSRAEKVAQKNATLPVLSGLSLEALGGILTI
                     RATNLDLGISIHIPVKVITEGQVVVPANIFSSLLNSLSKEKNVSLTLDKQVLLVETSS
                     TKSSIKTLPSEDFPIIPEIGGGSNFSIPTKDLITGLKSVIYAAAQGSIKPELSSVCLI
                     HEGENLVFVATDSFRLAEKKIKVKKIPNFNQILIPQKNAQEIIRIFDGLTEDVSISIE
                     ENQIAMRSGLVYLVSRTIDGSFPDYKQIIPKESRSKVVVLKQDLVSSLKTSLIFSDNF
                     NQLKIHISPKDKKFEIESKNQDVGENKEAVEAVIEGDEIGVNVNHRYFTDSFSSISSD
                     SLNLSFVGDGRPILIEGVGDKSFMYLLMPMNR"
     gene            10433..10693
                     /locus_tag="PLD99_00795"
     CDS             10433..10693
                     /locus_tag="PLD99_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25478.1"
                     /translation="MYSIGKFLERIKNSLDISSLNKDAVQTEILRLSGVVIDTKDIDI
                     KGDVLWLKTSPTKMTEIKLNEEKIIEATSRVLNQRVSKISFK"
     gene            complement(10695..12806)
                     /locus_tag="PLD99_00800"
     CDS             complement(10695..12806)
                     /locus_tag="PLD99_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRH25479.1"
                     /translation="MNPIKRFWGWKHRKNALSLGISVILLVFGLGFIWIASLKIPDLS
                     DLTERKVVESTKIYDRTGEILLYDMGQNIKRTLIPFKDISDYVKKAVVAVEDRNFYTH
                     GGFDFEALLRAVWVDIKTLSLSQGGSTISQQVVKNSILTMDKTITRKLKEIILSIKLD
                     GVLEKDEILNIYLNEIAFGGSLYGVEEASQSFYRKSAKDLTLAESAYLAAIINAPTYY
                     SPYGKNKDKLESRKNMVLREMKNQNLISEDEYTKALKEEVLFNPRENTGGIRAPHFVF
                     YVIDSLLDQFGEDALQNGGLKVVTTLDYDIQEKAEAVAKKYGEINQKNFNADNNAIVV
                     IDPKTGDILAMSGSRNYFDKDIDGNFNAATAHRQPGSTFKPIVYSVLFNKGYTPDTIL
                     WDVPTQFSPACAWNKFETDDTCYSPMNYDELFRGPMKIRDALAQSINLPAVQAVYLAG
                     IKNSLDFAEDLGIQSLGDTSRFGLSLVLGGGEVSLLELVSAYGVFANDGVRNPHNAIQ
                     RVYDGNGETVNEYSPSPRRVMPEETARMISSILSDNIARSPAYGLSSTLYIPSRPVAV
                     KTGTTNDYKDAWIVGYAPNLVIGAWVGNNENVPMEKKVAGFIVAPLWRELMDQVLPNF
                     DKESFEPMSPIDPNIKPILRGDLGSIGNHSILYFVDKDNPLGPAPINPSLDPQFENWE
                     NAVGAYVQFGNIRPPTPAPTL"
     gene            12837..13985
                     /gene="tyrS"
                     /locus_tag="PLD99_00805"
     CDS             12837..13985
                     /gene="tyrS"
                     /locus_tag="PLD99_00805"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRH25480.1"
                     /translation="MDNELRELLTRSVSEILPSKESLEETLLSGKKLRIYIGTDATGE
                     SLHLGHATNYMILERFRRLGHEVIFLIGDFTSRIGDPTDKSDSERKQLSREDVVKNTK
                     TWINQVKPVIDVDNKDNPVRIMYNHEWLSKLTLEDMLGLASNFTVQQMIERDMFEKRI
                     SSGKPLYLHELFYPLMQGYDSVAMDVDMEMCGNDQKFNALAGRTLLKKLKNKEKFVFI
                     TRLLENPTTKQKMMSKSLGTGVFLDFEPNKMFGALMSQPDENMQQLFVDCTWLKLEEI
                     EEITKLHPKEAKMRLAKEIVKIYHGVEKAREAEEYFEKTFSKKEIPQDIKVAKVSKDE
                     SLVNVLVSEGLVSSKSEFTRLEKAGAIEELENGVYRIGKHRFLKIERE"
     gene            complement(13986..14174)
                     /locus_tag="PLD99_00810"
     CDS             complement(13986..14174)
                     /locus_tag="PLD99_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25481.1"
                     /translation="MDEPINDDIAPIDPDDLDEIDGEEDKEHESLDALADKELDESDE
                     EEDEDEIDFEDGEEESLI"
     gene            complement(14229..14720)
                     /gene="ruvC"
                     /locus_tag="PLD99_00815"
     CDS             complement(14229..14720)
                     /gene="ruvC"
                     /locus_tag="PLD99_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRH25482.1"
                     /translation="MPNNEERIIGIDPGFDRCGVAIIEKTGGKEKLLYSTCITTNIKD
                     LHEKRLNKLGTELREIIGKWKPNSLAIEKLFFNVNQKTAIKVAEARGVIMYEASLANL
                     TVSEYSPQAIKIAVTGYGKATKPQVESMVVRILGIKSTKMLDDEMDAIAVGITHLASI
                     RHI"
     gene            complement(14710..15240)
                     /locus_tag="PLD99_00820"
     CDS             complement(14710..15240)
                     /locus_tag="PLD99_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRH25483.1"
                     /translation="MAGHNKWSKIKRQKEKTDGQKSKIFSKFAKAISTASREVKGDVN
                     SPTLRAAIEKAQEFNMPRENIERAVKKGMGGEGEQMDAITYEAYGPGGCALIIDALTG
                     NRNKAAQEVKFILKEFGIELAQPGSASWAFVKEGGEWKPTVTVPIGEADGEVLENLIE
                     KLEENDEVQDVYTNAE"
     gene            complement(15248..>15357)
                     /locus_tag="PLD99_00825"
     CDS             complement(15248..>15357)
                     /locus_tag="PLD99_00825"
                     /inference="COORDINATES: protein motif:HMM:NF017317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HRH25484.1"
                     /translation="QLGLIERTPRGRMLTVNGYNHIGLTPPKNLQNKLL"
     assembly_gap    15358..15457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15458..16431)
                     /gene="ruvB"
                     /locus_tag="PLD99_00830"
     CDS             complement(<15458..16431)
                     /gene="ruvB"
                     /locus_tag="PLD99_00830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRH25485.1"
                     /translation="MSSKIDKDTDTETGKDKSFLDTELRPSQWDEYIGQKAIKDNLRI
                     LLKAAAERSTAPEHVLFYGPPGLGKTTLAHLISKETGRQIKVTSGPAIEKVGDLASVL
                     TNLGSGDILFIDEIHRLNKTIEEILYPAMESGTLDIIIGKGPSARTIQLELPPFTLLA
                     ATTRVAAVSSPLRSRFSGGVYRLEFYTEQELEEIIRRSAKILGVETSGGAEKAIAKRA
                     RFTPRTANYFLKRARDYAQVHKTPLDDDAVTEALKLLGVDDVGLTPSDRKLLQTMADK
                     FSGGPVGLGTLAAALSEEEATIEEFNEPYLIQLGLIERTPRGRMLTVNG"
     gene            complement(16695..17081)
                     /locus_tag="PLD99_00835"
     CDS             complement(16695..17081)
                     /locus_tag="PLD99_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25486.1"
                     /translation="MNKIIIFILVVLIAGGFWYSSRPKQANLETLISNLMVEKDFPSV
                     SGYKIFESVENTSGKYTAVLFGAKNSESAFAVAVFEGEIVKKSFVLQEEGVGNKLENL
                     SWKSENQFSFDQTSSGSKVTKTLTIK"
     gene            complement(17086..18765)
                     /locus_tag="PLD99_00840"
     CDS             complement(17086..18765)
                     /locus_tag="PLD99_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HRH25487.1"
                     /translation="MIQFDEDKQNDRLDMLHKKEEEDLASTLSEKYGVSYLDLNAHPI
                     NIDALRVLKEDEARAAEIAVFNVVDKKIEVAVLSPESERTKEAIKSLRARGYVPEIFM
                     VSHASLNKVFERYKDLSYSFETKAGALDISNEEILLILSKVKYLKDVQVEIQTILGQK
                     KAYRVSKILEIILAGGIALHASDIHMEPSEKEVRLRYRLDGVLTDILEFDRETFDLLL
                     SRLKLVSNLKLNKDTAQDGRFSIKLADSAVEVRVSLLPGENGESVVLRVLSPESTLVT
                     LDHHDIHPKLLTIIKREIRKPNGMILTTGPTGSGKTTTLYSILKEIYSPDIKIITIEN
                     PIEYHLKGIVQTQVDEESGYNFAEGLRSSLRQDPDVIMVGEIRDKETATTAVNASLTG
                     HLVLSTLHTNNAAGTFPRLLDLGINPKVVSSAINIAIAQRLVRRLCDNCKKEVPLEGE
                     TKTLFDKVLDNIVDKSYLDGVQREKIWDAVGCPECNDSGFKDRLNIFEAILVNEKIEK
                     AVINNPSERDIETASRDQGLLNLVEDGFLKILLGQTSLEELKRVVDLSEVE"
     gene            18872..19279
                     /locus_tag="PLD99_00845"
     CDS             18872..19279
                     /locus_tag="PLD99_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25488.1"
                     /translation="MRKLFVLTLVLLTVSLGACTTIVQGGVSQVYSPVLQHTRVTVVN
                     NCSSEADLELGGQVVKSGLKYGESFTVVITRAGIVSQNSVDRVLIFKARIGETYLGSS
                     TRRYRLDANRSGNEEVWEVSNVRTANGSGGCGT"
     gene            19354..19797
                     /gene="tsaE"
                     /locus_tag="PLD99_00850"
     CDS             19354..19797
                     /gene="tsaE"
                     /locus_tag="PLD99_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRH25489.1"
                     /translation="MEYKSISIKETRELARNFVLTLNQNDNEATVVAMEGELGSGKTA
                     FTQELGKTLGITEHMQSPTFVIEKIYELVGSKWTHLVHIDAYRLKNEEELLALGFKKQ
                     LEDKHNLIVIEWASRVENILPKSAIHIVFTHDGEDQRTIEIKGQG"
     gene            19754..>21126
                     /locus_tag="PLD99_00855"
     CDS             19754..>21126
                     /locus_tag="PLD99_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRH25490.1"
                     /translation="MAKTKEPLKSKAKDKSEKKRLVLLDAHAIIHRAYHALPDFATSK
                     GVPTGALYGLNTMLLKIIEDLKPDYIVAAFDLPEKTHRHIAYDAYKGKRKETDENLAT
                     QLESAKSVFIAWGIPIYSVPGFEADDILGTIVELLKKDKNIEIVIASGDMDTMSLIDK
                     KKVQVFTLKKGLNDTILYDEDKVVERFGFGPELLPDYKGLRGDPSDNIIGVKGIGEKT
                     ATNLIQAFGTVEKIYKALKKNGDEYKKAGINERVRDLLVENEEEALFSKTLATIRRDA
                     PIDFKLPKVWREALDINILNTLFQELEFRTLPDRVRRLLNSSTSKDSAVLTRQNPTGS
                     DALESSRSLPEDEELKLALWVVDSNLTNPSMEDVATFTKADTLEDAWKVVNAELDKRK
                     SREVFEKIEKPLIPVVQEMNRVGVKIDKQMLTTLSTDYHKELSSLEKQIWEHAGEEFN
                     VNSPKQLG"
BASE COUNT         5876 a         4583 c         4666 g         5801 t
ORIGIN      
        1 aaattggtac caaatatccc aagcaatacg gtacactatg agggaggtaa agatccttat
       61 cgagaaccaa tataagaatt atgcgcttcc aagtaccaca atacatagag gttgaggaca
      121 aaatctttgg tcctcttacc ttcaaacagt ttatctacct agcgggtagc attggtattg
      181 ctgtcgtgct ccttactcta ctacctaggt ttatagccat aatccttgga gtgcctgtgg
      241 tcgtctttgg agtagccttg gccttcttta aggttaatga gcgccctttc accctagtca
      301 ttgaggactt tattaagttc tacatcggct ctcgtctcta tatatggaag aaggaagcca
      361 acaaaccaag tgatgcacac aactctatag agcctggtaa gtcgctcgaa caactctctg
      421 taccaaaact ctctggttca aagctcaaag atctaacatt cgaactagat gtgaagcaac
      481 aatctaatcc tgtaaccaaa gataggctat aatattctta tggctagtgg tgctcttcct
      541 actcaatcat ttgttcctat caaagaagtt cgtgatggtg tcatcgtcga gaaggacggt
      601 ggtatgcgag caatacttct cgcatcatca ttgaacttct ccctcaaatc tgtggatgag
      661 aagaacgcta ttttgctcca gttccaagac ttcttgaact ctttagactt ctctgtacaa
      721 attgtcgtcc agtctaggaa gctagacatc agaccctatc tcgccctcct agaagaacag
      781 agcaagctcc agaccaacaa cctgatgaag ctccagacca gagagtacat agagttcgtc
      841 cgctccttca ccgaatcgac caacattatg accaagaact tcttcatcgt cgtgccatac
      901 aaccctgcta ttgtctcaac aggcaagtct ggcggtctct cctcactcgt aagtcttagg
      961 cccaaggaca aggctggcgc caaggaagct agtttcgaag aggctcgtac tcagctagaa
     1021 gagcgtctcg ctgtggtgga gcaggggtta ataaggaccg gtatccgcat tgcaagactt
     1081 gggacggacg aggtaataga gctcttctac aaagccttca acccaggcga gacagagaag
     1141 gctacaataa aacgataata ttatgggatt tttcgacaca ttaatcggca aaaaggagga
     1201 aaagagtgag aacaggatcc aatccatact acctcaagaa gtctttgagg caggggtact
     1261 tgagctaaag gacctcatag ccccatcagc actcaaggtt acaccaagag agctcgacct
     1321 gggcgagaag atacttagga gtttctttgt catctcatac cctcgcttcc tctctgaaga
     1381 gtggttctct ccgatactta acatggacaa agtcttcgac gtctcgattt ttgtccaccc
     1441 tatagagact gcgaaaatac ttcgacaatt ccaaaagaag gtggccgagg ttgagagcca
     1501 gatacatagt agagaggaga agggtcttgt gcgcgacccg gtactcgatg tggcctacca
     1561 ggacctagag aatctgcgcg accagctcca gcaagcccag gaaaaactct ttgatgtagg
     1621 tctttacatc accatatatg ccgactctga cctcgagctc gataaaatag agaatgagat
     1681 caaatctatc ctcgaggcca agctcgtcta tgttaaaccg gctctcttcc agcaagagca
     1741 ggggttcaaa tctactcttc cgcttggtga cgacctgctt ggagtgcact ccaaacttaa
     1801 ttcatctcca ttatcgtcac tcttcccatt cacatccttc gacctcactt ctgacaaagg
     1861 aatcctttat ggtatcaaca gacacaactc ttctctcgtc ctcttcgacc gcttctcact
     1921 agagaactac aactcagtta tcttcgccaa gtctggtgcc ggtaagtcat actgcaccaa
     1981 gctcgaaatc ctaagaaccc tcatgttcga tacagaggtt atcatcatcg atccggagaa
     2041 ggaatatgaa tacatggctg aggcggtggg gggcagatac ttcaacatct cactcaactc
     2101 tgagcaccac atcaaccctt tcgacttacc agtacctact gcagacgagt ctgctgcgaa
     2161 cattttgcgt agcaacatta tcaatcttgt tggtctcttc cgcatcatga tggggggact
     2221 tacctccgag gaagacgctc ttattgaccg cgccatcacc gagacttatg cgctcaaaga
     2281 tattacagct gactctgact ttagaaatgt cgaaccacca ttaatgtctg acttcgagct
     2341 cgtgctctcg ggcctcgagg gaggggagtc gctcgcccag agactgacaa aatacaccaa
     2401 gggtacttgg gcaggattca ttaacaggcc taccaatgtc gatatcaaca aaaagcttgt
     2461 agtcttctcg cttcgcgata tggaagatga gttaaagcct gtagcaatgt acattgtcac
     2521 ccactttatc tggaactcag ttagaagaga gttgaagaag aggttgcttg tcatcgacga
     2581 ggcatggtgg atgatgaagt cagacgacac tgcgtccttc ctcacaggac tcgttaagag
     2641 agggaggaag tattatcttg gtgtagcaac tattacccaa gatgtgggtg acttcctcaa
     2701 atctccatac ggactaccta ttattacgaa ctcttctatc caagtcttat tgaaacagtc
     2761 tccgacgaac atcgacaatc tacagaaaac atttaacctt accgacgagg agaagttctt
     2821 gctcctagag tctgatgtgg gggagggtat cttcttcgct gggcttaagc atgtagctat
     2881 caagatcatt gcatcgtaca ccgaagacca gatcatcacc tcggacccaa gccaagtctt
     2941 agctcaaaag aaggcaagga gagagctcca agaaaccaaa gaagtaaatg tctaaagagt
     3001 taagtaacgg cacaatctta ctaatggtct caacagccat cttctttgat gtacttcaaa
     3061 ttgctttcgc cttaattgcc atggactggg tggtagggat attcggcttc ctaacctttt
     3121 ttatatggtt caagataaac ggagtgagct tcctcacacc aaaaagagct attaccatgt
     3181 caggttcagc tattattgaa gcctttcctt tccttagctc actccctact tggaccctgg
     3241 ctgtaatcat tattgctatg gacactaagg ccaagcaaat tgccaagaag acaataggta
     3301 atactgagga taataaagag aataaagcgt aacaaccttg ttataatgaa aggatgaaaa
     3361 aactgtttct tctacttgtt cttggcattt taataccaat atgtggaaca cgtgctgagg
     3421 gagaagtgga tcttatatgg gaggccagca cctttaccca cccattttac gaaggattgc
     3481 ctctttggag caacgagtca agcattacct taacggctat tcctcatctg cctaatataa
     3541 gtccaaacaa tattatttac cgatggagca agaacaaaac tgtcctcggc tctctctcgg
     3601 gtataggcaa aaacagttta tcaatcaaag atacagttct ctcgctccca gttgaggtca
     3661 cgattgatct cttcctggaa aacggtagta gtccaattgg tactgcaacg ttgtcactga
     3721 ctccaacaaa cccaaagctg cttatttttg aaaatagccc tttgtatgga atgctttcaa
     3781 acaaatctat tcaaaatgaa tttactatca aagaagacga ggtcactcta tctgcggtgc
     3841 ccctcttctc aactgtgagt aaaagaactg caggagcctt tgaatatacc tggcttacaa
     3901 actctggaga aagacgaata ggggactctg taacttacag agctccagag gggggtagtg
     3961 gctcggcgtc tgttgttgtt agagcaaata actctaaaat cattgctcag cctaagaatg
     4021 taaatttttt gataaaattc aatgaacaaa atgactttta aaaaactatt tatatttgga
     4081 attttaggat tgattttttt cttgccgaca ataaccttgg ctcaaaatac tggcgtcact
     4141 ggatacaccc ccctagcacc aatcccagag ctaatgaata aagcgggtaa ccaagtcgac
     4201 atcaactcat ttattcctgg agctattaaa ctggctatca gtattgcaag tgctttatct
     4261 gttatataca tcattgttgg tggagtgcag tacatcacta cggattcaat ggggggcaaa
     4321 agtaacgcca aaggaacaat agaaaacgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnt cactacggat tcaatggggg gcaaaagtaa cgccaaagga acaatagaaa
     4501 acgccattat gggtctgctt ctagctattg ggtcgtatgc aattctttac accataaatc
     4561 ccaaactggt cgtgtttgat ataaaacttt cggaacttga aggcgttggt atagacttcg
     4621 atggagcaag aaatggtcct attgttacac caacaactga agacataagt aaaagccttg
     4681 agggctgtaa tgattgtgct caaattccca gcggggttag atacaaaagt gctcaggcta
     4741 acggctgtgc aatgcctgga ccttgtgtgg taaatagaga ggttggccag aaattactaa
     4801 ctttaacaaa ctcattatca agaagcggta tatcatttca agtaactgaa tcttggccac
     4861 cgactagacc tcacgtagct tcttgtcaaa acccgaacaa cccagattct ggaacatgtg
     4921 ttgatgcaaa ctttattaat gccataaatt tagcctcccc gagtgacaga acaaaggtag
     4981 aaagttttat tttggctgct caagcggccg gattaagagc tgtgtatgaa gtaaccaata
     5041 ttggagatag agatagattg attagcggca acttagattc cggtgacgtc atagctccga
     5101 gaatgaatgt gtgtgtgagt ggcgagacaa ccgcattaaa cgctagtaca aacagatatt
     5161 ctccttgttg tcccacaaaa gaattaaaaa acaatccagg aaacattaca tgcagttgga
     5221 ttagtgctaa tcacttctct gtttacaata aatagttatg tcgaaaaaac ttacaatctt
     5281 tataatcatc ctcaccatcc ttcttggtag tgggatactc tactacctct tgggagggaa
     5341 ggggccaagt ggattaggag ggatacctgg actgccattc ggtgaagcac ctggagatga
     5401 tgtcggcggt actaataata gtgatccaaa cgatactcca aatgatggag aaacacctat
     5461 agacaccgtc ggaggaggca cagatggtgc caaactaacc aagatcacaa gcgaacctgt
     5521 tgctggtgca ataacattta taaacaaagg cagtacttat ctgcgttatg tagatcgcgc
     5581 aacaggtcat gttagcgagg ttaagctctc taccatggag aggaccaaga tcctaaacat
     5641 caccagacca aagatctacg aggctctttg gaagaaggac ggcactggat ttatagaaag
     5701 aactcttgat ggtgaggaga atatagtaaa cacatcggtt agcctaattc aaagcacatc
     5761 aacctcaagc accacagagc catatatcat tcgttcaacc ttgcttaagg gtgaggtggg
     5821 ggagatcaag ccagggttcg atggtggtct tgtatacaac cttactgatt caggcgttgt
     5881 ggccacatcc aacttcatcg gtgagaagcc gaagacccta ttcactctgc catttacctc
     5941 atggaggatc atgccagtat caaccactac agcactgctt accaccaaac caagtgctat
     6001 aagtcaggga tatagctact tgaccaatct aagtaccggc aaattaacca agatactcgg
     6061 accattgagt ggtctctcgg ccaagattaa gtctgactcg tcacgagtaa tttatagtta
     6121 tatagataac aacaaaattg agttgaaagc taaagatcta aaaaacggtg tagtgacaga
     6181 catattacca aagactcttg ctgataaatg tgtgtggagc aagaaagctg gcgcagttct
     6241 atattgtgga gcaccaaaga gcttgcctca aggactgcct gactcttggt accaaggaga
     6301 agtcggtttt gccgacagtt tctggagatt taatactgag acaaatactg cggaggagtt
     6361 atatgtccca caagctgaga gcgaaggtgt tatagactca cactcaatag agcttagccc
     6421 tgacgaggat tatctaatat ttgtgaacaa gtccgacttg tcactctggg ttcttaagct
     6481 tccgctttaa gtttgtttct gacaaatatc tcttgagcaa tagtaaagat gtttgttgtg
     6541 agccagtata gagcaaccac tcccgaaatc gtatatgcaa taaagaagac cataacaggg
     6601 aagaagtact tcatctgagt ttgcatactc tttgcaagat catcgccaaa ggaagctgcg
     6661 ccactcttaa ccttaggctt aactggagag gcaaacttga tttggaagaa agtgctaata
     6721 gcagcaagaa gagcaagaac cacactcttc gcagttaaat ctgctccaag gaagaaaggg
     6781 ctcacagtct ctggcacact aacgaacgaa tacagtaaat cagctctaat ctcagggaag
     6841 ccggttgata taaatatatg atacaaagcg aagattacag ggagctggat tagcaagact
     6901 aagatacctg agaaaggatt cacccctcgc tctttgtata gtgccaaggt cctcctcgct
     6961 tgctcctcct gattatcttt gtactttgcc ttgatactct ctagttctgg ggcaaggttt
     7021 tgcatttcaa cctgagctct caccgccttc ctagagatag ggaagagagc cagtctaact
     7081 atcagtgtta tacaaataat agcgagacct gcgccaaaag atggaaaaag gttaaatatc
     7141 gctacaaagg tattgtagat cggttcgact agtaggttgt tgtatagggc tgacacaaat
     7201 gaattctatc gttttttgaa ctgatttaga agcgaagtga tttctttttc taatttctct
     7261 cccttaagtt ttttcgcact tggtcttgca acaaataggt aggtgttggg ttctagctct
     7321 tttacagagt cgcggacgat tgagtagact cttcttctca aggtatttct gtctacagcg
     7381 agcttagaaa ccttcttaga tacagaaaca gcgagtttag gcttactctc tccagatcca
     7441 acacggagag agaagttctc tgaattaaca aaccgcttgg aagtaaggag atttgtaaaa
     7501 tccttcctct ccaggcggtt ttgtttagaa agcattaggc agttaggcga gctcgtccct
     7561 tgcggcggcg agctgtgacg gttcggcgac caccggcagt ggccttcctc ttcatccagc
     7621 cgtgggctct agctctcttg cgcttctttg gttgatatgt tgtagacata tattaatcaa
     7681 caggttgtga acacattata aacttttttc cgcttcaata caagtttggg atgatccacg
     7741 gttccgtata tactacatac gtttcaataa ctctttttat ggatcaacga caactttggg
     7801 aaagcatatt ggcagaaata gaactttcag tttctaaacc tgcctttgca acctggttta
     7861 aggacaccca tatcgtcaag agagacgaaa ataccattat tctctctgtt ccaaatgcct
     7921 tcgctaggga catgttccaa tctaggtacc acgccactgt tctcaagctc ctcagggata
     7981 aggatcacac catccatgct gtagagtatg taataaaccg agatgagccc aaaagaagag
     8041 acgaaaagat catcaatacc caaaacggca atctcccttt acccgaaact ctggtcaaca
     8101 aggacgacaa cctaaacccc aggtatacct tcgaaaactt cattatcggc ccttttaatg
     8161 agctagctca tgcggccgct caggctgtta ttaagtctcc aggactcatg tataacccgc
     8221 tatttatcta cggctccaca ggccacggta agacccacct gatccagtct atagggaacc
     8281 acataaagac cgtctcaccg ggcaaaaggg tcttttatat gacctcggag aagttcggcc
     8341 aagactgcat gaatgccctc caaacccaaa aaatggctgt atttaaggag aaatacagaa
     8401 aatacgatgt tttgatcatg gatgacatcc aattcttctc cgaaaaacag aagttccaag
     8461 aagagctgtt ccatctcttt aacaccctct acgacaacaa taagcaggtt atattctcct
     8521 cggacaaaca cccccacttc atacaaggct tagaagacag gctaaggtcc agaatgtcgg
     8581 ctggcatgat cgttgatata ccccaaccag accacgagtc taggatagcc atcttgaatg
     8641 ctaagtcgaa gctccaaaac atctccttat ccccagacgt catcgactac ctagccaaga
     8701 cagtagtggg taacattaga gatctcgaag gggccctaaa cacactcatc atccactctc
     8761 aactcaaagg tcgcgaagtt gctgtggccg atgttaggaa cctcatcaaa agtaaccaga
     8821 agccaaaacg cgctgtggct gttaaggaca tcgtaaaggt catatcggac ttttattctg
     8881 tagaggaggg tagtatctac gaaaagacaa ggaggaagga agtgatcaaa ccaagacaaa
     8941 tcataatgta cattttgaga gaagacttta atatctcata tccttcaata ggggagaagt
     9001 taggtggtag ggaccacacg acggttatcc actcctgtga gaagattaaa gaggacctaa
     9061 agaacgacca gtccttgggt caggagcttt cgcagattag gaacctttta tctgtttagt
     9121 tactgtagat aaactgttca caactacccg aaatgaagga acaagtaaga gcaaaaaaga
     9181 attcacagga aatgttgtta gaagtttgcc gaacaaatcc ccaagttttg tttttaaatt
     9241 tttctaaaaa ccttttctct agaacatata atgaagtttt gcacatatcc acacccctag
     9301 tattagtaac aagtatttat atatgaaaat agaatctatt aaagaaaagc ttgtggaagc
     9361 agttagtaga gcagagaaag tagctcagaa gaatgcaact ctcccagttt tatcagggtt
     9421 atcgctcgaa gctcttggag ggatactcac aatacgagca acaaatcttg atcttgggat
     9481 ttcgatacat attcctgtta aggtcattac tgaaggacag gttgtagtac cagcaaatat
     9541 tttttcctca cttctcaact ctttatctaa agagaagaat gtctctttaa ctttagacaa
     9601 acaagtcctg ttagttgaga caagtagcac caagtcatca attaagacat tgccaagcga
     9661 ggattttcca atcatcccag aaattggtgg tggaagtaat ttttctatac caacgaagga
     9721 tctaattaca gggttgaagt cagtgattta tgctgcagca caaggaagta ttaaacccga
     9781 gctttcttcg gtctgtttaa tacacgaagg agaaaatctt gtcttcgttg ccacagactc
     9841 attcagatta gcggagaaga agattaaggt gaagaagatt cccaacttca accaaatact
     9901 tattccacag aagaatgctc aagaaataat caggatcttt gatgggttaa ccgaagatgt
     9961 ctcgatcagt attgaggaga atcaaatcgc tatgagatca ggactcgttt acctagtttc
    10021 tagaacaata gatggtagtt tccctgacta taaacagatt atccctaagg agagccgatc
    10081 taaagttgtt gttttgaagc aagatctagt ttcatcactt aagacctctc tcatcttctc
    10141 tgataacttt aatcagttaa agatccatat ttcgcccaag gacaagaaat tcgaaataga
    10201 atcaaagaac caagatgtgg gggagaataa ggaggctgta gaggccgtta ttgaaggaga
    10261 cgagattggt gtcaatgtta atcaccgata ctttaccgac agcttctctt ctatatcaag
    10321 tgatagttta aatttgtctt ttgttggaga tggtcggcct atattgattg agggggtggg
    10381 ggacaaaagc tttatgtatc tcttaatgcc gatgaatcgg taagaggagt ttatgtactc
    10441 tattggtaaa tttctagaga ggataaaaaa ttctcttgat ataagtagtt taaataaaga
    10501 tgctgttcag actgagattt tgagactctc aggggttgtg attgatacca aggatataga
    10561 tattaagggt gatgtgctct ggcttaagac cagccccacg aagatgacag agataaaact
    10621 taatgaagaa aagattatag aggcaacttc tagagttttg aatcagagag tttcaaaaat
    10681 ttctttcaag taagttagag tgttggagcc ggtgttgggg gcctgatatt gccgaactgt
    10741 acataagcgc ccacggcgtt ctcccagttt tcgaattgtg ggtcaagact tgggtttatg
    10801 ggggccggtc ccaaagggtt atccttgtct acgaagtaga ggatcgagtg gttgccgatg
    10861 gagcctaggt ctccacgaag aattggtttg atgtttggat ctattggtga cattggttca
    10921 aaactttctt tatcaaagtt tggcagtact tggtccatca actctctcca gaggggggcg
    10981 acgatgaatc ctgcaacctt tttctccata ggaacattct cgttgtttcc gacccaggcg
    11041 cctattacaa ggttgggggc gtatcctact atccaagcat ccttataatc gtttgtggta
    11101 cccgttttga ctgctacagg cctactaggg atgtagagtg ttgagctcag accatatgct
    11161 ggagaacgag cgatattatc ggacaatata ctcgagatca ttctggctgt ttcttcgggc
    11221 ataactcttc ttggagaagg tgagtattcg ttaacagtct ctccgttacc gtcatatact
    11281 cgttgtatgg cattgtgtgg gttccttaca ccatcgtttg caaacactcc gtatgcggag
    11341 actagttcga gtaatgaaac ctcaccgcct ccaagaacga gcgatagccc aaaacgactt
    11401 gtgtctccta agctctgtat tcctaggtct tcggcaaaat ctaatgagtt cttaatacca
    11461 gcaagataaa cagcttgtac tgctggcagg ttgattgatt gtgctaaggc atctctaatc
    11521 ttcattggtc cgcggaagag ttcgtcatag ttcatcggag agtaacaagt gtcgtcagtt
    11581 tcaaacttgt tccaagcaca agcaggggag aattgtgtgg gtacatccca aagtatagtg
    11641 tctggcgtgt aacccttgtt gaagaggaca gagtagacta tgggtttgaa tgttgatccg
    11701 ggttgtctgt gtgccgtagc tgcgttgaag ttgccatcta tatccttatc aaaataattt
    11761 cttgatccag acatagcgag gatatcgcct gtttttgggt ctataaccac aatcgcattg
    11821 ttatctgcgt tgaagttctt ctgatttatc tctccatatt tcttggcgac agcctcggct
    11881 ttctcttgga tatcgtagtc tagagtagta acaaccttca acccgccgtt ctgtagtgcg
    11941 tcctcgccaa actgatcgag caggctatcg attacataga agacgaagtg cggggcacgg
    12001 atgccgccag tattctccct ggggttgaag agcacttctt ctttgagtgc ctttgtgtat
    12061 tcatcctcac ttattaaatt ctgatttttc atttctctta gaaccatgtt ttttctgctc
    12121 tctaatttgt ccttgttttt gccataagga gagtagtaag tgggtgcgtt aatgatcgcc
    12181 gcaagatacg cagactctgc aagagtcagg tccttagcac tcttcctgta aaagctttgt
    12241 gacgcttcct caaccccgta aaggcttcca ccgaaggcaa tctcgtttag ataaatattt
    12301 agaatctcat ccttctctag gacaccgtca agtttgatag agaggattat ctccttaagc
    12361 tttctggtaa tggttttgtc catggtgagg atagagttct ttaccacttg ttgagagatt
    12421 gttgagcccc cttgggagag agacagggtc ttgatatcaa cccacaccgc gcggagtaat
    12481 gcctcaaagt cgaatcctcc gtgtgtgtag aagttcctgt cctcgaccgc gaccacagcc
    12541 ttcttcacat agtcagagat atctttgaag gggatcaggg tgcgtttgat gttttgcccc
    12601 atgtcgtaga ggagaatctc gccagttcgg tcatagatct tggttgactc gaccaccttc
    12661 ctttctgtca ggtcagagag gtctgggatc ttgaggctcg ctatccaaat aaaacctagg
    12721 ccaaaaacca agagtattac cgaaataccc aaagaaagcg cgtttttcct atgtttccag
    12781 ccccaaaacc tttttatagg gttcataaaa ctatcttaac atgttagatt actcgtatgg
    12841 ataatgaatt aagagaactc cttaccagat cagtttcgga gatcttgcct tctaaagagt
    12901 ctttagaaga aaccctcctc tcagggaaga aactccgtat ctatatcggc acagatgcta
    12961 ctggggaatc tctccacctt gggcacgcaa ccaactacat gattcttgag aggtttcgta
    13021 ggttgggaca cgaagttatt ttcttgattg gagattttac ctctaggata ggagacccga
    13081 cagacaaatc agattcagaa agaaagcagc tctctcgcga agatgtcgtc aagaacacaa
    13141 agacttggat caaccaagtt aagcctgtga tagatgtgga caataaagac aatccagtaa
    13201 ggataatgta caaccatgag tggttgtcca aacttacttt ggaagacatg cttggtttgg
    13261 catcgaactt caccgttcag caaatgatag agagagacat gtttgagaag cgtattagta
    13321 gtggcaagcc tttgtatcta cacgaactct tctatcctct tatgcagggg tacgactctg
    13381 tggccatgga tgtagacatg gaaatgtgtg gaaatgatca gaaatttaac gctttggcag
    13441 gaagaactct tttaaagaaa ttaaagaata aagagaagtt cgttttcatc acaagacttc
    13501 ttgagaatcc tacaaccaaa cagaaaatga tgtctaagtc tcttggcaca ggagtgtttt
    13561 tggacttcga gccaaacaaa atgtttggag cactaatgtc tcagccagat gagaacatgc
    13621 aacaactttt tgttgattgt acttggctca aattagagga gattgaggaa ataacaaaac
    13681 ttcatcctaa ggaagccaag atgagattgg caaaagagat agtgaagatc taccacggag
    13741 tggagaaggc tagggaagca gaggaatact ttgagaaaac tttttctaaa aaagagatcc
    13801 cgcaagatat taaggttgca aaggtctcta aagacgagtc tttagtaaat gtattggtaa
    13861 gtgagggact tgtatcttct aaatccgaat tcactcggtt agagaaagct ggagcaattg
    13921 aggaattaga aaacggtgtc taccgcattg gcaaacaccg tttccttaaa attgagcgag
    13981 agtagttaaa ttagggactc ctcttcaccg tcttcgaagt ctatttcgtc ctcgtcttct
    14041 tcctcatcac tttcatcgag ttccttatca gcgagggcat ctaaactttc gtgttctttg
    14101 tcttcctccc catcaatctc atcaagatca tcaggatcga taggtgctat gtcatcgttt
    14161 attggttcgt ccatgtattt aaattttgtt taattatgct ccacagacag tggagcaggt
    14221 tatttgtatc aaatgtgcct tatcgaagca aggtgggtaa tccccacagc aatggcatcc
    14281 atctcatcgt cgagcatttt tgtactctta attcctaaaa tccttaccac catgctctct
    14341 acctgaggct ttgtggcctt accgtagcct gtcacagcta tcttaatagc ctgaggacta
    14401 tactccgata cagtgagatt agctagcgaa gcttcgtaca tgatgacgcc cctggcttcc
    14461 gctactttga tagcggtttt ttggtttaca ttgaagaaaa gcttttctat tgctagagaa
    14521 ttgggtttcc atttgccaat aatctcgcgg agttctgtac caagtttgtt taatcttttc
    14581 tcatgcagat ccttaatatt tgtggtgatg cacgtagagt agaggagttt ctctttgcca
    14641 cccgtttttt cgattattgc tacgccacat ctatcgaacc ctggatcgat accaattatt
    14701 ctttcctcat tattcggcat tagtgtagac gtcttggacc tcatcatttt cttctaattt
    14761 ttcaatcaag ttctcgagca cctcaccgtc agcctctccg attggtacag tcacagtcgg
    14821 cttccactct ccaccctctt tcacaaacgc ccatgaggca gaacctggct gagcaagttc
    14881 gataccgaac tccttaagaa tgaacttcac ctcttgagcc gccttattcc tgttaccagt
    14941 tagggcatca atgattagtg cgcatccacc tgggccatat gcttcgtagg tgatggcgtc
    15001 catttgctct ccctctcctc ccataccttt cttcacggcg cgttcgatgt tttctcttgg
    15061 catgttgaat tcctgagcct tttctatcgc cgctcgtaga gtgggggagt tcacatcacc
    15121 cttgacctct ctcgaagcgg tggaaatagc cttagcaaac ttagaaaaga ttttggactt
    15181 ctgtccatct gtcttttcct tctgcctctt aatctttgac catttgttat gtcctgccat
    15241 tgtgttttta taaaagctta ttttggaggt tcttcggagg agtaaggcca atgtggttgt
    15301 atccgttaac cgtaagcatt ctaccccttg gagttctttc tattagaccg agttgtannn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg ttaaccgtaa gcattctacc
    15481 ccttggagtt ctttctatta gaccgagttg tattaagtac ggctcgttaa actcctcaat
    15541 agtggcttct tcctccgaga gtgctgctgc aagtgtgcca agtcctacag gtccaccact
    15601 aaacttgtca gccatggtct ggaggagctt tctgtcggat ggtgtaagtc ccacatcatc
    15661 aactccaaga agttttagag cctctgttac agcgtcatcg tctaatggtg tcttgtgtac
    15721 ctgggcatag tcacgagctc gcttcaagaa gtagtttgct gtgcgagggg tgaagcgagc
    15781 tcgcttggcg atagctttct cagctccacc acttgtctct actcctagaa tcttggctga
    15841 gcggcgaatg atctcctcca attcttgttc tgtataaaac tctaatcgat atacaccgcc
    15901 ggagaagcgc gagcgaagag gggatgacac cgccgctaca cgagtagttg cagcaaggag
    15961 ggtgaacgga ggaagctcaa gttgtatggt gcgagcactt ggacccttac cgataataat
    16021 gtcgagtgtt cctgactcca tagcagggta tagtatctcc tcaatggttt tgttcagtct
    16081 gtggatctcg tcgataaaga gaatgtctcc tgagcctagg tttgtgagta ctgaggcaag
    16141 gtcgccaacc ttctcaatcg caggacccga tgtcaccttg atttgtctcc cggtttcttt
    16201 ggatatgagg tgagcgagag tcgttttacc gagtcctggt gggccgtaga agagtacgtg
    16261 ctctggcgca gtagatcttt ctgccgcagc cttgagcaaa atgcgcaaat tatccttaat
    16321 tgctttctgt ccaatgtact cgtcccactg actagggcgt aattctgtat ccaaaaagct
    16381 tttatcctta cctgtctcag tatctgtgtc cttatctatc ttacttgaca tagaaaatat
    16441 tatatgctag ggttagtccc ggcacaagac agacttcttc acaggttttc cagatcatac
    16501 atctctaagc aatgcccccc gcgggctttg agcgtcttac tgaggacgtg cgtggttcgc
    16561 ttgcgaactg ccgctatcct taagtctcac gcttattgct tggagatgta cgctctggaa
    16621 tctccagtac tcatttacgc atagcctcaa gctatctgca atctacccaa gcacccttta
    16681 tcgtgtctta ctgactactt gattgtaagg gtcttggtaa ccttagatcc agaacttgtt
    16741 tgatcgaagg agaattggtt ctcagacttc caagatagat tttctagctt gttgccgaca
    16801 ccctcctctt gaagaacaaa agacttcttc acaatctcac cctcgaagac tgccacagca
    16861 aaagcgcttt cgctgttttt tgcaccaaag aggacggcgg tatatttgcc cgaggtgttc
    16921 tcaacacttt caaatatttt gtagccacta acagaaggga agtccttctc taccatgagg
    16981 ttagagatta gtgtctcgag gttggcttgc ttaggacggc tggagtacca aaaaccgcct
    17041 gctatcaaaa ctactaagat aaagattatt attttgttca tattgttatt ctacttctga
    17101 caaatctacc actcttttca attcttccaa actggtttgt cccaagagta ttttgaggaa
    17161 gccgtcttca actaagttta gaagtccttg gtctcgactt gctgtctcga tgtctctttc
    17221 tgagggattg ttgataactg ctttttcgat cttttcattc accaggatcg cttcaaaaat
    17281 gtttaaccga tccttaaagc cagagtcatt gcactctggg cagcccaccg cgtcccaaat
    17341 cttctctctt tgtaccccat caagatagct cttgtcgaca atattgtcga gcactttgtc
    17401 gaaaagtgtt ttggtctctc cctctaatgg cacctccttt ttgcagttat cgcagagtct
    17461 tctaactagt ctttgagcga tagcaatgtt gatagcacta gatacgactt taggattgat
    17521 accaagatca agcaggcggg ggaaggtacc agcagcattg ttggtatgta gtgttgagag
    17581 cacgaggtga cctgtaagag aggcgttcac cgcggtagtt gcggtctctt tgtctcggat
    17641 ctcgccaacc atgatgacgt ctgggtcttg gcggagcgag gagcggagtc cttcggcgaa
    17701 gttgtaacca ctttcttcgt caacttgtgt ctgcacaatg cctttcaagt ggtactcgat
    17761 aggattctcg atagtgatga tcttgatatc tggagaataa atttctttta gtatcgagta
    17821 aagagtagta gtcttaccgc ttcctgttgg tcctgtggtt aggatcatgc cattcggctt
    17881 cctgatctca cgcttgatga ttgtaaggag cttggggtgg atgtcgtggt ggtcgagagt
    17941 gacgagtgta gactcggggc tcaagactcg gagtactact gattcaccgt tctctccggg
    18001 gagtagggag actcgcactt cgacagcgct atcagcaagt ttaatggaga agcggccatc
    18061 ctgagcagtg tctttgttta gctttaggtt agatacaagc tttaatctgg agagcagtaa
    18121 gtcgaaagtc tctctgtcga attcgagtat gtcagtgagc actccgtcta agcggtatct
    18181 tagtctcacc tccttctcac taggttccat gtgtatgtct gatgcatgga gagcgatacc
    18241 gccagctaat ataatttcta ggattttaga aactctgtaa gccttcttct gtcctaggat
    18301 tgtttgtatc tcgacctgga catctttgag gtacttaact ttactaagga taagaagaat
    18361 ctcttcgtta gaaatatcta gcgcaccagc tttggtctcg aatgagtaag acagatcttt
    18421 gtatctctca aaaactttgt taagagatgc atgagagacc atgaagatct ctggcacata
    18481 accccgggct cggaggctct ttattgcttc cttagttctt tcgctttcgg gggagagaac
    18541 cgcaacctca atcttcttgt cgacaacgtt aaaaaccgca atttcagctg ctcgcgcctc
    18601 gtcttccttg agcactcgta gggcatcgat attgataggg tgggcgttga ggtcgaggta
    18661 gctcacaccg tacttctctg agagagttga ggctaggtcc tcctcctcct tcttgtggag
    18721 catgtctaga cggtcatttt gcttgtcttc atcgaattgg atcatggcta cattctacct
    18781 caaggagccc tttatagagc cctcatttac cccttgactt ttacctataa agtatggtat
    18841 agtaggtcaa gaacccttac aggagatcag catgcggaag ctcttcgttt tgaccctcgt
    18901 tcttctcacc gtgagccttg gcgcttgcac caccatcgtg cagggtggag tgagccaggt
    18961 gtatagcccg gtcctccagc acacgcgggt caccgtggtg aacaactgtt catccgaggc
    19021 tgacctcgag ctcggcgggc aagtggtcaa gtccggtctc aagtacggtg agtccttcac
    19081 cgtcgtcatc actcgggctg ggatcgtttc ccagaacagt gttgatcggg tgctcatctt
    19141 caaggcacgg atcggggaga cctacttggg atcttcgact cgtcggtacc gcctcgatgc
    19201 gaatcgttcg ggcaacgagg aggtctggga ggtgagtaat gtccgcaccg cgaatggtag
    19261 cggaggttgc ggaacctgaa ggagacggtg ctcaaaactg gggcaggagg ttctaacaag
    19321 aacttcctgc ccttttcgtt tatatttata tacatggaat acaaatcaat tagcattaag
    19381 gagactcggg agctggccag gaacttcgtc ctcactttaa atcagaatga caacgaggcc
    19441 acagttgttg ctatggaggg ggagctcgga agtggcaaga ccgccttcac ccaagagttg
    19501 ggcaaaacgc ttggtattac cgagcatatg cagtctccaa cctttgttat agagaagatc
    19561 tatgagttag ttggcagtaa atggactcac ttggtacata tagatgctta caggctcaaa
    19621 aatgaggagg agttactagc tctaggcttt aagaaacagc tcgaagataa acataatttg
    19681 atagtcatag aatgggcctc gagagtcgag aatattttgc ctaagagtgc tatacatata
    19741 gtattcacac acgatggcga agaccaaaga accattgaaa tcaaaggcca aggataagtc
    19801 agagaagaaa agactcgttt tgcttgatgc gcatgccatt atccaccgtg cgtatcatgc
    19861 actgcccgac ttcgctactt cgaaaggtgt accaacaggt gcgctctacg gtttaaacac
    19921 catgctcctc aaaatcattg aggatttgaa gccagactac attgttgctg cttttgattt
    19981 gccagagaag acacatcgtc acatcgctta tgatgcttat aagggcaaaa gaaaggagac
    20041 ggatgaaaac ttagccactc agcttgagag tgcgaaaagt gtcttcattg cgtgggggat
    20101 accaatatat tctgtacctg gattcgaggc agacgacatc ttgggcacca tagtcgagct
    20161 tttgaagaag gataaaaata tagaaatagt tattgcatca ggtgatatgg acactatgag
    20221 ccttatagac aagaagaagg tacaagtttt cactctaaag aaaggtctta atgacacaat
    20281 tctttatgac gaggataagg tggtagagag atttggcttc ggaccagagc tcttgccgga
    20341 ctacaaagga cttcggggcg atccgtcaga caatatcatt ggcgttaagg gtataggaga
    20401 gaagacagca accaatttga tccaagcctt cggtacagta gagaagattt ataaagcgct
    20461 taagaagaac ggggatgagt ataagaaagc gggtatcaat gagagagtaa gagaccttct
    20521 tgttgagaat gaggaggaag cgctcttttc taaaaccttg gccaccatac gtcgtgatgc
    20581 accgatagat tttaaattac caaaagtttg gagagaggcg ctcgacataa atattttaaa
    20641 cacattattt caagaacttg aatttagaac tttaccagat agggtgagga gattgttaaa
    20701 cagctcgact tcgaaggatt ctgcggtctt aacccgtcaa aatccgacgg gctccgacgc
    20761 cctcgaatct tctcgaagtc tccccgaaga tgaagaactc aaacttgctc tctgggtagt
    20821 ggactcaaac ctcaccaacc cttctatgga ggatgtggcg accttcacca aggcagacac
    20881 tctcgaggac gcatggaagg tggtgaatgc cgaattagat aagaggaaga gtagagaggt
    20941 gtttgagaag atagagaagc ctctgatacc agtggtccaa gagatgaata gagtgggagt
    21001 aaagatcgat aagcagatgc ttaccactct ctccacggat tatcacaaag aactcagttc
    21061 tttagaaaag cagatttggg agcatgcagg cgaggagttt aatgtgaact cgccaaagca
    21121 gttagg
//
LOCUS       DAORIT010000006        20885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_401_length_20885_cov_51.191887, whole genome shotgun sequence.
ACCESSION   DAORIT010000006 DAORIT010000000
VERSION     DAORIT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20885
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_401_length_20885_cov_51.191887"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..734
                     /locus_tag="PLD99_00860"
     CDS             123..734
                     /locus_tag="PLD99_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25491.1"
                     /translation="MNVDKQTVQKQIYRGGRTHFDIGKLPAKIKTLTVLLGYLKFIFG
                     VLKSIEGYKLSDRLKYLVDESNKRLAILAKKSISLQPISEADIMKEIRFVKDAIFAYY
                     ESFEGFMRSATVRFSGELEKMRKQNPEIVDRFSGQLADLQRVFRDKLENEKVSYDDLV
                     SAHNAVDLLLDEISLEIEEAETARVLKENEAEARELLEALQAA"
     gene            825..2348
                     /locus_tag="PLD99_00865"
     CDS             825..2348
                     /locus_tag="PLD99_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25492.1"
                     /translation="MSFSPNKILFDRDKGTLLLSISKEDVSIPEFLVSKAKQNNFKRK
                     EEFHITVFGFGAGQRILKKLDVEPGSKEVFLNKIQELSEGTNWEIDLTNQFYHIKKTY
                     KNKNGVEEHRESYITMVKMGNISNFVKEVSNLINTHIDVPPPHTTLFSKSSIEENMLA
                     GIGVGSEEDLKKLKPAQLSEESDKPAKIKTIALPTRSQPDTIIAIFILKQFGGDKFVG
                     IGDADYGVMERMKDGETNETLMQQGILAVDIGGGMFDHHSKPFQTTASNLVSEYLGVR
                     DNPALSKLLQFAERDDFFGKGIISNDPLDRAFGLSGLIGSLNKKYVNEPGKVIEIVLP
                     IIEAFYAEENKRAFEMPQEYEEKVKSGKAQIFEVKQRGRLLKCIFIETDNASMAGFLR
                     SKGGGGFDVVALKLTSRHINILTKPITKPDLRSLTVLVRIQEAEAKGIALEGEPEKLS
                     TPGTMKEVPEWYYDPATNSLLNGGPNPQEIKPTSIDPFEFKKILEVGISEQLWSPGR"
     gene            2444..2977
                     /locus_tag="PLD99_00870"
     CDS             2444..2977
                     /locus_tag="PLD99_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25493.1"
                     /translation="MPQPNVLFKSQRFNLTRFMASLSRMGLELNRAHMKRNDGQGKTG
                     QIMLVFEKGMPPMSKAKYDELVGTFIGLEGAEDTGFAWTVQAFDNEAISRVPAHVFIN
                     CGNPQEKKGDEPNLSMRIQKLADVAKKKTMVRIAFNLYPDEMHLIPSWVTERPRHTER
                     VSERESAAELLEATQGF"
     gene            3129..3473
                     /gene="rpsS"
                     /locus_tag="PLD99_00875"
     CDS             3129..3473
                     /gene="rpsS"
                     /locus_tag="PLD99_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRH25494.1"
                     /translation="MTRSSKKGPYVDPRVMKKIEGKKPGETGVIKTWSRACQIAPEMI
                     GFTFGVHNGRDHVEVFVTEDMIGHRLGEFSLTRKFVKHGGKMQKDIESKAKEAEINAA
                     KAAKASTDTKKK"
     gene            3478..3855
                     /gene="rplV"
                     /locus_tag="PLD99_00880"
     CDS             3478..3855
                     /gene="rplV"
                     /locus_tag="PLD99_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRH25495.1"
                     /translation="MTEAKATLKNYRQSPRKVRVVADLVRGKRVEDALVTLMYTTKRA
                     TDPLSKLLSSAIANAKNLSIPTENLVVKEISVDGGAILYRRLPKSRGMATPIRKRTSH
                     VTITLAEGKPKKVKSVKAKVAKK"
     gene            3857..4528
                     /gene="rpsC"
                     /locus_tag="PLD99_00885"
     CDS             3857..4528
                     /gene="rpsC"
                     /locus_tag="PLD99_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRH25496.1"
                     /translation="MSHTVHPYSHRLGILRDWKSRWFSPKGKFKEYLEADVVIRRYLE
                     KKLKGFFIADIEIERSQKALRIVLSTSRPGMIIGRSGDGSQKLLADVKKLLEKKKLLP
                     AKVELKLDVKEVKSPESNAAIVAEMVVDGLEKRMPFRRVMKSMLEKVMANRDVQGVRI
                     FLAGRLGGATMSRTEDRKLGRIPLQTFRADVDYACTKAVMPYGAIGVKVWIYRGEIFD
                     KDAQK"
     gene            4540..4962
                     /gene="rplP"
                     /locus_tag="PLD99_00890"
     CDS             4540..4962
                     /gene="rplP"
                     /locus_tag="PLD99_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRH25497.1"
                     /translation="MLFPKKVKYRKWHTMRKNPLKVGPATRGITVAFGSYGLKAMDQV
                     RIKSNQIEAARKVLSRALGKTGRLWIRIFPDMPYTAKPAEVKLGKGKGDLQGYVAPVR
                     AGRMLFEVDGVSEAIAKEALRKAGTKLPVKTKIVAREQ"
     gene            4964..5206
                     /gene="rpsQ"
                     /locus_tag="PLD99_00895"
     CDS             4964..5206
                     /gene="rpsQ"
                     /locus_tag="PLD99_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018673892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRH25498.1"
                     /translation="MNTKAHKKKVFTGTVVSTKMKDTVVVLVERYEKHPKYEKFMKSS
                     KRFKAHDAGNTAKVGDKVSITETKPISKDKHFILTK"
     gene            5208..5579
                     /gene="rplN"
                     /locus_tag="PLD99_00900"
     CDS             5208..5579
                     /gene="rplN"
                     /locus_tag="PLD99_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRH25499.1"
                     /translation="MIQPRSIVEIADNSGAKIGRIFKVLGSSKKRYARIGDIVVLSVQ
                     KAEPRKQVKKKDVLYAVVVRSRAPMKRSDGSYVRFDENAVVILDNSKEEPMAGRVFGP
                     IPRELGERGFQKIISLAPEVV"
     gene            5591..5806
                     /gene="rplX"
                     /locus_tag="PLD99_00905"
     CDS             5591..5806
                     /gene="rplX"
                     /locus_tag="PLD99_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRH25500.1"
                     /translation="MHIKKNDNVIVLTGKDKGKTGKVLRAFPKLNRVLIEGINIKKVH
                     QKKTKKDGKGQIVEKSFPVDASNVKVK"
     gene            5811..6359
                     /gene="rplE"
                     /locus_tag="PLD99_00910"
     CDS             5811..6359
                     /gene="rplE"
                     /locus_tag="PLD99_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRH25501.1"
                     /translation="METLKTKVGKAFKEMKSHGYKNAMQSPKIEKVVVNVGVGSMKDK
                     NKMKIVEDRLMKITGQKASPRGAKVAIANFKSRQGDIVGYQVTLRGARMYDFLERLLH
                     IALPRTKDFRGISKASADEMGNYTLGIKEHTIFPETADEELKDVFGLSVTVVTSAKSR
                     VEVQDFLEYLGFPFKKVEEKKK"
     gene            6434..6622
                     /locus_tag="PLD99_00915"
     CDS             6434..6622
                     /locus_tag="PLD99_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25502.1"
                     /translation="MSNKKVRYPVPLSEIVLTPEEQKFLTPEFVEETTRGAIRYVEER
                     RSVTESPEFSVPRTKPAK"
     gene            6729..7322
                     /locus_tag="PLD99_00920"
     CDS             6729..7322
                     /locus_tag="PLD99_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25503.1"
                     /translation="MQILGGVFYTLNKIFFSFKERSVGERARQWRIWAWVVYIIGLVP
                     WLVILSHEQNWMVVFVEAGGVPAMFLGLTLALRREDENPSKRSRLFEKFFNQTAVTAA
                     VLGIAYSLYDVGIMTSVSQWAELGVTIGFLVGTFRLAKDHLDGYLWFLLMNASAGLLM
                     WLQGYDWLALQQAVSLAFVLDAYYFKRRGRAKIRLMR"
     gene            complement(7831..8073)
                     /locus_tag="PLD99_00925"
     CDS             complement(7831..8073)
                     /locus_tag="PLD99_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25504.1"
                     /translation="MSDNSDHGVSEEELDEFFAKGSKYDVAFISGLSTREIQRRVLGR
                     LKQMIPGLRQIQAEIEASGHITAKDWQSRCNVTGIY"
     gene            8168..8353
                     /locus_tag="PLD99_00930"
     CDS             8168..8353
                     /locus_tag="PLD99_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRH25505.1"
                     /translation="MAKKSVIARSKKTPKFSTRATNRCFRCGRGRAYMRDFGLCRICF
                     RELANEGKIPGIKKSSW"
     gene            8364..8750
                     /gene="rpsH"
                     /locus_tag="PLD99_00935"
     CDS             8364..8750
                     /gene="rpsH"
                     /locus_tag="PLD99_00935"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRH25506.1"
                     /translation="MDPISNMIVSIKNAGDAGRDTIVIPYSKLKHDIAKILVEEKYIK
                     SASKKGSEKKPTLEIELYMDKRTPKVKGVKRLSKPSKRVYRKVGDIRPVKQGYGILVL
                     STPAGIMAGYKAKKAGVGGEALFNIW"
     gene            8769..9302
                     /gene="rplF"
                     /locus_tag="PLD99_00940"
     CDS             8769..9302
                     /gene="rplF"
                     /locus_tag="PLD99_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRH25507.1"
                     /translation="MSRIGKQIITIPEKTEVTVTRELVTVKGPLGELKRKLHPVIDVK
                     VSGTEVTVHPKKETLESRALWGTFASHIKNMVNGVNKPFEKKLILEGIGYKSEVKGDK
                     IVFALGFSHPVEVTIPKELKVTAEKNNITVSGIDKELVGGFAADMRALKKPEPYKGKG
                     MRYSDEVIKRKQGKKTA"
     gene            9319..9636
                     /gene="rplR"
                     /locus_tag="PLD99_00945"
     CDS             9319..9636
                     /gene="rplR"
                     /locus_tag="PLD99_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002816352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRH25508.1"
                     /translation="MNTQKSQKRIRNHKKIRTQVAGTSERPRLAIFKSNTAIYAQVIN
                     DDAGVTLASAKGTDAKKVGAEVAKAAQGKKIESVVFDRGGYIYTGKVKDLADSAREAG
                     LKF"
     gene            9642..10295
                     /locus_tag="PLD99_00950"
     CDS             9642..10295
                     /locus_tag="PLD99_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRH25509.1"
                     /translation="MEDTKVQENTAVETTPVEVAESVVADVVAVPAQAADPRNSRGGD
                     NRRGPGGPRRDNRRGPRRDTRPKSEFDQKIVSLRRVTRVVAGGRRFAFSVCVVAGNRK
                     GMVGVGQGKATDTPLAIEKAFRDAKKNMISINMTKSMSIPHEVEAKYAASRVKIMPAP
                     GKGILAGSSVRAVLELAGLKEISSKLLSRSKNTINNAYVAIKALEQLKRTKIKAKDR"
     gene            10306..10746
                     /gene="rplO"
                     /locus_tag="PLD99_00955"
     CDS             10306..10746
                     /gene="rplO"
                     /locus_tag="PLD99_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRH25510.1"
                     /translation="MQLHTLKSKTKRKKSRQVGRGGKRGKTSGKGTKGQNARAGRKKR
                     PELRDFIKRFPKLRGRGVSSFKSFQDKAVVINLKALDEAYKVGETVSVPTLLEKKLID
                     MKNGKATKVKVLATGDLSKKLDFEGLLVSESAKSKIEKAGGTIR"
     gene            10751..12025
                     /gene="secY"
                     /locus_tag="PLD99_00960"
     CDS             10751..12025
                     /gene="secY"
                     /locus_tag="PLD99_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRH25511.1"
                     /translation="MFKTFIDKVGLVLSDSVLKRRILITIGILVLARVLAVVAIPGID
                     QSALASFFSGNQFLSLLNIFSGGGLASLSIIMLGVGPYITASIIMQLLTLMIPNLKDM
                     QQDGGDSGRQKLSQYGRLLSVPLSFLQAYAFISLFQKQGVLPALSTGTLVLDILLITA
                     GSMLLVWLGELITEFGIGNGISLIIFAGIVSSLPAAIAQLAFTFDPSQIPVYIAMFVV
                     TILLIFGVVIVSEAERPIPITYSKQIRGGATSGGMQSYLPLRVNQAGVIPIIFALSIL
                     LFPQMILQFLTSSTVASVASAATSILALLNNLWFYNSAYFLLVFLFTYFYTAVTFDPE
                     QIAENLQKSGAFVPGIRPGEPTAEYISRVLTRLTLIGALFLGVVAVLPLVLRSVTQIQ
                     SMAIGGTALLIVVSVVIDLIKKIDAQLSVREY"
     gene            12046..12645
                     /locus_tag="PLD99_00965"
     CDS             12046..12645
                     /locus_tag="PLD99_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRH25512.1"
                     /translation="MLHTVVFIGRSGCGKGTQAELLRNRIKEHDLKKRQILYIETGDG
                     FREFVRGKSLSSRLSKAIYDRDARQPDFLATMMWGKMLLEQLDEEMHLVFDGVARSKA
                     EAHMLSTALWFYEREKPTIIYINVSRKWSEEKLLKRGRADDVNLARIDKRLDWFDKDV
                     IPSIEYFKENPFFRVIEVNGEQSVEAVHAELISKYEYSE"
     gene            12642..13409
                     /gene="map"
                     /locus_tag="PLD99_00970"
     CDS             12642..13409
                     /gene="map"
                     /locus_tag="PLD99_00970"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRH25513.1"
                     /translation="MIRLKTKDQIQTMHEGGKRHSEILRALAEMVTPGMSTLILEEEA
                     RRLIRAHGDTPAFLNYSPKGASRPFPAALCVSINDEIVHGIPNEKERILKDGDIVSLD
                     LGLKHEGFITDSAVTVPVGSVDDESRDIMRLAKEALEAGIEAAKPGAHIGDIGEAVYK
                     VVKGTGFSLAEDLAGHGVGFEVHEDPFVPNIGRKGEGEKLVPGMVIAIEPMVNAGGSA
                     IADTDDGYTIVTADGSRSAHFEHTIAITETGNIVLTR"
     gene            13457..13882
                     /locus_tag="PLD99_00975"
     CDS             13457..13882
                     /locus_tag="PLD99_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25514.1"
                     /translation="MFAKILLGLIMAIVALGFLAEWYYHWIRRVQPVDHSDPFAVPDL
                     DKYAVEDSSPTPRAEAFFRANNEAASEMGMTLAGYSALIIKSAAESPYPTNKCLTESE
                     LENVAKQRFGLISPREADHAYHCKYCRSGVLWYRSRGPR"
     gene            13939..14541
                     /locus_tag="PLD99_00980"
     CDS             13939..14541
                     /locus_tag="PLD99_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012185494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRH25515.1"
                     /translation="MSHPKKERSFVAVKHDGVQRTLVGEIINRVERTGLKIIGLKMFV
                     PSMPQTVEHYGKDDAWCEKVGQRTIDNMAKEGKTTDKTAVEIGREVLNALYKYFTLGP
                     VVGFVVEGNKAVDIVKKLVGGTEPTTSDVGTIRGDYTLDSYDLANVDGRAVRNLIHCS
                     DKPEEAEREIKIWFKNEELINYKSIQEAILYDVNIDGILE"
     gene            14817..15215
                     /locus_tag="PLD99_00985"
     CDS             14817..15215
                     /locus_tag="PLD99_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25516.1"
                     /translation="MSRSILMSALFFSVLVGVLNKIGGIYFLYWGLSWFDMLMHFLGG
                     VTLALFFIWGYYYSGFLSRNVTSDKLYFFKIICLVMLVGLGWEVFEFYFDIAHPTRGK
                     YYEDTFLDLFFDFLGVWSVSLLIKFKEKKI"
     gene            15212..16531
                     /locus_tag="PLD99_00990"
     CDS             15212..16531
                     /locus_tag="PLD99_00990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRH25517.1"
                     /translation="MKTSLTCYGGVGTVTGANFLLEVESGGKILRVLIDCGLFQGLIA
                     EDDVSKTFDYDVTTIDHLFITHAHIDHIGRIPDLIKAGFKGKILSTRETKDIARFMLK
                     DLSHIAGEGDDAAEKALSMWEIHPYHTKKLFELGDEEVFSLELFDAGHILGSAMYKLT
                     FPSGKSILFSGDIGNSPAPLQSDVERVSDATYMLMDSVYGDRNHESIEERDKRFAEII
                     AEVIERKSTLLIPVFSLERTQVMIHELDKLFTSKGLKSIPVFLDSPLAINITEIYRDR
                     FNSKDKDLYFNKLQETAQVRDSISISHVPGPKIIMAGSGMSTAGRIVHHEERYLPDPN
                     ATILFVGYQAAGTLGRLIQDGIKEVVINDKKIKVNARVLSIGGFSAHADSDQLVKFVE
                     ASEKTLEKVFITMGEPKSSIFLAQRLKDELGVDSVIPEKGKVYELDL"
     gene            complement(16562..16906)
                     /gene="rplT"
                     /locus_tag="PLD99_00995"
     CDS             complement(16562..16906)
                     /gene="rplT"
                     /locus_tag="PLD99_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRH25518.1"
                     /translation="MTRVKKGVNALKTRKNILRKVKGYRFGRSTKERQANEAIFHAGT
                     YAFAHRRDKKGDFRRLWNVRISSAVKPLGLSYSKFIDALNKKGIKLDRKILADLAENH
                     PTVFVKIVEAVK"
     gene            complement(16911..17105)
                     /locus_tag="PLD99_01000"
     CDS             complement(16911..17105)
                     /locus_tag="PLD99_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRH25519.1"
                     /translation="MSIKTNKSFAKRIKVTKKGKILVRKTGQNHFNAKESSSEKVAKG
                     KLGEFHMSNKERSRFLVNIK"
     gene            complement(17157..17663)
                     /gene="infC"
                     /locus_tag="PLD99_01005"
     CDS             complement(17157..17663)
                     /gene="infC"
                     /locus_tag="PLD99_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRH25520.1"
                     /translation="MLTPRTNHAIKSKEVRVIGPEGENFGVLNLDEALKKAKEFDLDL
                     VEISPNANPPVAKITDLGKYMYAEAKKEKESKKKAHSVEVKSIQVKLGTGDNDLGLKA
                     KKASEWLAEGNRVQINLFLPGRAKYLDEKFLKERIERLLKLVTIPHKIADAPKKSPKG
                     MTAIIEKA"
     gene            17731..18186
                     /locus_tag="PLD99_01010"
     CDS             17731..18186
                     /locus_tag="PLD99_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HRH25521.1"
                     /translation="MEIKHTLPDLEQIIRARHPLKNSFVKTQETLSSAERFAVYVTHH
                     VGSFRFFTILLIWSFLWLAWNLFGPVELRFDPAPAFVIWLFLSNIIQLILLPLILIGQ
                     NLENRHTNMQAEEDYQVSKHSEEEIKIIIGHLENQNELMLEIMRKLDRN"
     gene            18377..18544
                     /locus_tag="PLD99_01015"
     CDS             18377..18544
                     /locus_tag="PLD99_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25522.1"
                     /translation="MQHELEVVDVVEKNGKKVAKIKHQCDPTRLITLTETAVGWVDEL
                     GVKYPADYIEV"
     gene            complement(18608..18853)
                     /locus_tag="PLD99_01020"
     CDS             complement(18608..18853)
                     /locus_tag="PLD99_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25523.1"
                     /translation="MSVLDLVLGFDNLWAVMILVTFLPLLLYCIQSEKNDAKRKKNKQ
                     VPMFDSILESFFHRTTGVMCTLSFLAILIKIGIRFLK"
     gene            complement(19241..>19593)
                     /locus_tag="PLD99_01025"
     CDS             complement(19241..>19593)
                     /locus_tag="PLD99_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25524.1"
                     /translation="VPKDQKSAIAEDIALSELPETSEHPHDGIPKPTSMLEMMLLGIL
                     EPTVTAPAGTYDMMIADGRATLFVNGEERWQRKFSTKSTAKRTVSEGVELGYIGKEDE
                     TKMMKQIEESTLPE"
     assembly_gap    19594..19693
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19694..19875)
                     /locus_tag="PLD99_01030"
     CDS             complement(<19694..19875)
                     /locus_tag="PLD99_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25525.1"
                     /translation="MCGCNPNVQHGYFHLPTGDKASPTFYSKGRALELLEEVVADGRV
                     PKDQKSAIAEDIALSEL"
     gene            complement(20090..20326)
                     /locus_tag="PLD99_01035"
     CDS             complement(20090..20326)
                     /locus_tag="PLD99_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25526.1"
                     /translation="MSTLAYKKHLEEKKGEDVSVDRRDTSIVKGRVDAVSDEAVEISY
                     RKPGTVDARIHTFVALDDIRGVSIEGWDHDIIAH"
     gene            complement(20439..20801)
                     /locus_tag="PLD99_01040"
     CDS             complement(20439..20801)
                     /locus_tag="PLD99_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25527.1"
                     /translation="MGNRGTTPSWDDEQITKFGIFGHFSDVGRRLRADMRRSDRQAMF
                     RMMIRKYRGALRCWGYENEPLITANNGEFRVGHLLTFLDRAEELLEMHHDDAAEYLIK
                     FIFGQLLEFKLMTPPYSD"
BASE COUNT         5571 a         4627 c         5237 g         5350 t
ORIGIN      
        1 tcgagcccct ttttgctatc cataaagtgc ttgacaagta gactgaagta tgctagggtt
       61 aatacagtag tttcccgttc cttcgcagta caacctagat aaccccaaac ggggaggata
      121 agatgaacgt cgacaagcag accgtccaga agcagatcta ccgcggtggt cgcacccact
      181 tcgacatcgg caagctgccg gcgaagatca agaccctcac cgtgctcctc gggtacctga
      241 agttcatctt cggggtcctg aagtcgatcg agggctacaa gctctcggac cggctcaagt
      301 acctggtcga cgagtcgaac aagcgcctcg cgatcctcgc caagaagagc atcagcctcc
      361 agccgatctc cgaggcggac atcatgaagg agatccgctt cgtcaaggat gcgatcttcg
      421 cctactacga gtccttcgag ggcttcatga ggtcggcgac ggttcgcttc tccggcgaac
      481 tcgagaagat gcgcaagcag aaccccgaga tcgtcgaccg gttcagcgga cagcttgctg
      541 atcttcagcg ggtcttccgc gacaagctcg agaacgagaa ggtctcgtac gatgacctcg
      601 tctcggcaca caacgccgtc gacctcctcc tcgatgagat ctcgctcgag atcgaggaag
      661 ccgagacggc ccgagtcctc aaggagaacg aagcggaagc tcgcgagctc ctcgaagctc
      721 tccaggccgc ctgatctact gcacaacgca cgctaatgcg tgcccgcccc acgaaagcct
      781 cacggcaatc gtggggcttt ctctttatgc tatagttttt ataaatgtca ttcagtccga
      841 ataaaatact tttcgataga gataagggaa cactcctgct ttctatctct aaagaagatg
      901 tctctatacc agagttttta gtaagtaagg caaagcagaa taattttaag agaaaggagg
      961 aattccatat tactgtattc ggttttggtg caggtcaaag aatattaaag aaattggatg
     1021 tagagccggg tagcaaggaa gtttttttga ataagatcca agagttatca gagggaacga
     1081 actgggagat agatttgacc aatcagtttt accacatcaa gaaaacttac aaaaataaaa
     1141 atggtgtaga ggagcatcgt gagtcatata taaccatggt gaagatgggt aatataagta
     1201 acttcgtgaa agaagtatca aacctaatta atacccacat agatgttcca ccacctcaca
     1261 ctactctttt ttccaagagc tcgatagaag agaacatgct tgcaggcata ggggttggct
     1321 ccgaggagga tttgaagaag cttaaacccg ctcaattaag tgaggaatcg gataaaccag
     1381 caaaaataaa aactatcgcc ttaccaacac gctctcagcc agacacgatt attgctatat
     1441 tcattttgaa acagtttggt ggtgataagt ttgtcggtat tggtgatgca gattatggag
     1501 taatggagag gatgaaggat ggggagacaa acgagactct catgcaacaa ggaatattgg
     1561 cagtggatat tggtggtgga atgttcgatc atcattcaaa accatttcaa accacagctt
     1621 cgaatcttgt atcagagtat ctcggagtta gggacaaccc tgcattgtcg aagttgctcc
     1681 agttcgcaga gcgagatgac ttcttcggca aggggattat atctaacgat cccttagacc
     1741 gagccttcgg actctcaggt ctaattggtt cgctgaataa gaaatatgta aacgagccag
     1801 gcaaggtgat cgagattgtc ctccccatta ttgaagcttt ttatgctgaa gagaataaga
     1861 gagcatttga aatgcctcaa gagtacgagg agaaggttaa gtcaggtaag gctcagatct
     1921 tcgaagttaa gcagagaggg aggctgctca aatgtatttt tatcgagaca gacaatgcca
     1981 gtatggctgg gttccttagg tcgaagggcg ggggcggctt cgatgtggtt gccttgaagc
     2041 tcactagcag acatataaat attttgacaa aacctattac caagcctgac ctaagatctc
     2101 ttacagtact tgttcgtatc caagaggcag aggcgaaggg tatagctctc gaaggcgagc
     2161 ccgagaaact ctctacaccg ggaacaatga aagaagttcc agagtggtac tatgaccctg
     2221 cgactaactc acttttgaac gggggaccaa atcctcaaga aataaaaccg acgtccattg
     2281 atccttttga gttcaagaaa attttagagg ttggaatctc cgaacaactt tggtcgcccg
     2341 ggaggtaagt tcatgcttga caaatagctc gaatatgcta taattgaaaa agagtaggga
     2401 aacctatcag atctcccaca aaatacccac acacgaggaa acgatgcccc agccgaacgt
     2461 gttgttcaag agtcaacggt tcaacttgac ccgcttcatg gcctcgctca gtcggatggg
     2521 ccttgagctc aaccgcgccc acatgaagag gaacgatggt caggggaaga cgggtcagat
     2581 catgctcgtc ttcgagaagg gtatgcctcc gatgtcgaag gcgaagtacg acgaactcgt
     2641 cggcaccttc atcggtctcg agggcgctga ggacaccggg ttcgcctgga ccgtccaggc
     2701 tttcgacaac gaagccatct cgcgtgttcc ggctcatgtc ttcatcaact gcggcaaccc
     2761 gcaggagaag aagggtgatg agcccaatct ctcgatgcgg atccagaagc tcgctgacgt
     2821 ggcgaagaag aagacgatgg ttcgcatcgc cttcaacctc tatccggacg agatgcacct
     2881 catcccgagc tgggtcaccg agcgtccacg tcacaccgag cgggtgagcg agcgcgaatc
     2941 ggcggcggag ctcctcgagg cgactcaggg gttctgatcc gaccaagggt cctttatcgg
     3001 gccaggccgt cacattgtga cggcctttta cttactttag ccccgctagg ggtggggtag
     3061 gtttgacaat agagccccat ttctatacta tatttgccta cgagcgccta agacgctttt
     3121 tgttttaaat gactagatcg agcaaaaaag gaccatatgt agaccctcgc gtgatgaaga
     3181 agatcgaggg caagaagcca ggcgaaaccg gggttattaa gacctggtca cgcgcatgtc
     3241 agattgctcc agagatgatc ggattcacct tcggagtaca caacggtcgc gaccatgtcg
     3301 aagttttcgt tacagaggat atgatcggtc acaggcttgg cgagttctca ctcactcgca
     3361 agttcgtgaa gcacggaggt aagatgcaga aggatattga gtctaaggct aaggaggccg
     3421 agatcaacgc agctaaggca gctaaggctt ctaccgatac taagaagaaa taattttatg
     3481 accgaggcta aagcaacact aaagaattat cgtcagtcac caaggaaggt ccgtgttgtg
     3541 gcagacctcg taagaggtaa gagagtagag gacgcactcg ttactttgat gtacaccacc
     3601 aagagagcta ctgaccctct aagtaaatta ctttcatcag caattgcgaa cgctaagaac
     3661 ctctctatcc ctacagagaa tctagtggta aaggagatta gtgttgatgg gggagcaatt
     3721 ttatacagga gactaccaaa gagtcgtggc atggctactc caataaggaa gcgcaccagt
     3781 catgttacta tcactcttgc cgaaggtaag ccaaagaagg ttaagtctgt taaggctaaa
     3841 gtagctaaga aataatatgt cacacacagt acacccatat tcacatagac tagggatcct
     3901 cagagactgg aagagtcgat ggttctctcc taagggcaag ttcaaggagt acttggaggc
     3961 tgatgttgtt atccgccgtt acctagagaa gaagctcaag ggtttcttta tcgcagatat
     4021 tgagatcgag agaagtcaga aggcactccg cattgttctc tccacctcac gtccaggtat
     4081 gatcattggt cgttctggtg atggctctca gaagcttctt gccgatgtga aaaaactgct
     4141 tgagaagaag aagttgctcc cagctaaggt cgagctcaaa ctcgatgtga aggaggttaa
     4201 gagccctgag tctaacgcag ctatcgttgc tgagatggta gttgatggtc tagagaagcg
     4261 catgccattc cgccgtgtga tgaagtctat gctcgagaag gtgatggcca accgcgatgt
     4321 acaaggagtg aggattttcc tcgcaggtcg tttgggcgga gctacaatgt ctcgaaccga
     4381 ggacaggaag ctcggacgca tcccacttca gaccttcaga gcagatgttg attacgcttg
     4441 caccaaggct gttatgccat acggcgctat cggagtgaag gtctggatct atcgtggaga
     4501 gatcttcgat aaggacgcgc agaaataatt tttactatca tgcttttccc taagaaagtt
     4561 aaataccgaa agtggcacac catgaggaag aatcctttga aggtgggccc agctacccgc
     4621 ggtatcaccg tcgctttcgg ctcatatggt cttaaggcta tggatcaggt tcgtatcaag
     4681 agtaaccaga tcgaagcagc caggaaggtc ctctcaagag ctcttggtaa gacggggaga
     4741 ctttggattc gcattttccc tgatatgcct tatactgcaa agccagctga ggttaagcta
     4801 ggtaagggta agggcgatct ccagggttat gtggcacctg tccgagctgg tcgaatgctt
     4861 tttgaggtag acggagtgtc tgaggctatc gccaaggagg ccttgagaaa ggcgggtacc
     4921 aagctccctg tgaagactaa gatcgtcgct cgcgaacaat aatatgaata caaaggcaca
     4981 caagaaaaag gttttcactg gcacagtcgt ctcaacaaag atgaaggaca ctgtcgttgt
     5041 gcttgttgaa agatatgaga agcatccaaa gtacgagaag tttatgaagt cttcaaagag
     5101 attcaaggct catgatgctg gcaacactgc caaggtgggg gacaaggtgt ctatcaccga
     5161 gaccaagcca atttctaagg ataaacattt catcttaacc aagtaatatg atccagccac
     5221 gctcaatcgt agagatcgca gacaactccg gtgcaaagat cggcagaatc ttcaaagtac
     5281 tcggatcttc gaagaagcgt tatgctcgca tcggtgacat cgtcgtgctc tcggtgcaga
     5341 aggccgagcc aagaaagcaa gtgaagaaga aggatgttct ctacgcggta gtagtgcgca
     5401 gccgtgctcc aatgaagcgc tctgacggat catatgtccg cttcgatgag aacgcagttg
     5461 tcatcctcga caactctaag gaagagccaa tggcaggacg tgtcttcgga ccaatcccta
     5521 gagagctagg agagagaggc ttccagaaga ttatttctct agcgcctgag gtcgtttaat
     5581 aaataccgcc atgcatatca agaagaacga caatgtaata gtgctcacag gtaaggataa
     5641 gggcaagacc ggcaaggttc ttcgcgcctt tcctaagcta aaccgcgtgc ttatcgaggg
     5701 tataaatatc aagaaagtgc accagaagaa gaccaagaag gatggtaagg gtcagatcgt
     5761 agagaagtct ttcccagtgg atgcttctaa cgtaaaggta aaataatctt atggaaactc
     5821 taaagacaaa ggtaggaaag gcattcaagg agatgaagtc acatggctac aagaatgcta
     5881 tgcagtcgcc aaagatcgag aaggtcgttg ttaatgttgg tgttggctca atgaaggaca
     5941 agaacaagat gaagatcgtt gaggatcgtc tcatgaagat cactggccag aaggcaagcc
     6001 cacgaggtgc taaggtcgct attgctaact tcaagagtcg tcagggtgac atcgttggtt
     6061 accaagtaac tctaagaggt gctcgcatgt acgacttcct cgagcgtctt ctacacattg
     6121 cactccctcg taccaaggac ttccgaggta tctctaaggc ttctgcagac gagatgggta
     6181 attacactct tggtatcaag gagcatacaa tcttccctga gacagcagac gaagagctta
     6241 aggatgtctt cggtctctct gtgactgtgg tcacttcagc taagagcagg gtagaggtcc
     6301 aagacttcct cgaatacctc ggcttcccat tcaagaaggt agaagagaag aaaaaataat
     6361 ttcaaaaata ttgttttgac ttaatagcgc gaaggcgcta ttttgtttgg agacacacat
     6421 ctcaggagac atcatgagca ataagaaggt gaggtaccca gtacctctga gcgagatcgt
     6481 gctcactcca gaagagcaga agttcctcac acccgagttt gtcgaggaga cgactagggg
     6541 cgcaatcagg tatgtcgagg aaagacgctc tgtcaccgag tcacccgagt tcagtgtacc
     6601 gaggaccaag ccagcgaagt aacgctggtt tttttcattt gtttgacaca gctagatatt
     6661 tggtgcaatg tgactttaga caaggtcccc tacatggagg cagagtgtct ctcgaactca
     6721 ggttctggat gcaaatctta ggaggggtgt tctacaccct gaacaagatc ttcttcagct
     6781 tcaaggaaag aagtgttgga gagagggctc gacagtggcg aatctgggcc tgggtcgtat
     6841 acatcatcgg actcgtccca tggcttgtga tactctcaca cgaacagaac tggatggtcg
     6901 tctttgtcga agcaggtggt gtgcccgcca tgttcctcgg actcactctg gctcttcgaa
     6961 gagaggatga gaacccgagc aaaagaagta ggctgtttga gaagttcttc aaccagactg
     7021 ctgtcactgc tgcagttctc ggcattgctt acagtctgta cgatgtcggg atcatgactt
     7081 cagtaagtca gtgggctgag ctcggggtca ctattgggtt ccttgttggc acatttcgtc
     7141 tcgccaagga ccatctcgat gggtatctct ggttcctgct catgaacgct tctgctggct
     7201 tgttgatgtg gctccagggt tacgattggc tagcactaca acaggctgtc tctctcgcat
     7261 tcgtgcttga tgcttattac ttcaagcgga gagggcgagc gaagattcgg ctgatgagat
     7321 aatcaccagc ctttttcata taaaacgaca taaatttaat cacttgacaa tagttgtgaa
     7381 gtgtgtataa taaatatgta cggtgagagt ctggcagaag ccgggcactg ccgtatgaag
     7441 tacaatattc atttccttac accggtgacg gaatggttcg actctcgaga cagagtcaga
     7501 atggttcccg atgggatgaa gccggtaact ccctgcaagc acatgcccga aaggggtgcc
     7561 caagatgttc ttcagaagaa gatggcgagg tttccgcctc acgaggctgg gggagggttc
     7621 ccccttcgtc atccacagag acaggagttt cggcattcta gatgcctagt gggggatagt
     7681 gatgtctcaa aacatttcac ttcctccata aacaaaactt cttcatctct gtgctaatca
     7741 catattgtgg cctaggccac tcacaataca gcgctcaccc tcgggttgag cgctttttca
     7801 atgttataaa aaacccacgc actgcgtggg tcaatagatt ccggtcacat tgcaacgact
     7861 ctgccagtct ttagcagtga tgtgcccact tgcttcgatc tctgcttgga tctgccgtaa
     7921 gccggggatc atctgcttta accggccaag aacccttctt tggatctctc gtgtcgatag
     7981 accactaatg aaggctacat catatttgct gcctttggcg aagaattcat cgagctcttc
     8041 ctcgcttaca ccatggtccg aattgtcgct catttagcct ccagaaatag ggcttaagta
     8101 cgctcttgaa tgattatatc ccgtatggta agctagcacc acgcctaaaa gaggcctttt
     8161 attttctatg gcaaagaagt cagtcatcgc aagaagcaag aagaccccga agttctccac
     8221 tcgggctact aaccgttgtt tccgttgtgg tcgaggtcgt gcttatatgc gtgacttcgg
     8281 actctgccgt atctgcttta gagagcttgc caatgaaggt aagatcccag gtataaagaa
     8341 gtcctcttgg taattccaaa tatatggatc ctatctcaaa catgatcgtg tctatcaaga
     8401 atgccggcga tgctggtaga gacactattg ttatccctta ttcgaagctc aagcacgata
     8461 tagccaagat cctagtcgag gagaagtata ttaaatcagc atcaaaaaag ggtagcgaga
     8521 agaagccaac cctcgagatc gagctctaca tggacaagcg cactccaaag gttaagggtg
     8581 tcaagagact ctcaaagcct tcaaagcgtg tgtacaggaa ggtaggtgac atccgccctg
     8641 ttaagcaagg ctacggtatc ctcgtccttt caactccagc cggcatcatg gcgggctaca
     8701 aggcgaagaa ggccggagta ggtggcgaag ctcttttcaa tatctggtaa tccaattttt
     8761 ataaacacat gtcacgcatc ggcaaacaaa ttatcaccat cccagagaag acagaagtaa
     8821 ctgtcactag ggaacttgtc actgttaagg gacctcttgg cgaactcaag aggaagctcc
     8881 atcccgttat agatgtaaag gtaagcggta ctgaagtaac agtacatcct aagaaggaga
     8941 ctctagagag tagggcactt tggggcacat tcgcatctca tatcaagaac atggtaaatg
     9001 gtgtgaacaa gcctttcgag aagaaattaa tactcgaggg tatcggctac aagtcagagg
     9061 ttaagggcga taagattgtt tttgcgctcg gcttctcaca cccagttgag gttaccattc
     9121 ctaaggagct caaggttacc gcagagaaga acaacatcac tgtctcagga attgataagg
     9181 agcttgttgg tggattcgcc gcagacatga gagctttgaa gaagccagag ccttacaagg
     9241 gtaagggtat gcgttactct gacgaggtta ttaagaggaa gcagggcaag aagacggctt
     9301 aatcttaaac ataccaacat gaacactcaa aaaagccaaa agagaatacg aaaccacaag
     9361 aagatccgaa ctcaggtcgc cggtacttcg gagcgtcctc gtttggcaat cttcaagtcg
     9421 aacactgcta tctatgcaca ggttattaat gatgatgctg gtgttactct agcttctgct
     9481 aagggtactg atgcaaagaa ggttggtgcc gaggtggcca aggcagctca ggggaagaaa
     9541 attgagtctg tggtcttcga ccgaggtggt tacatttata caggtaaggt gaaggacttg
     9601 gccgatagtg ctcgcgaggc cggactaaag ttttaaatat tatggaagat acaaaagtac
     9661 aagagaatac agcagtagag acaactccag ttgaggtggc agagagtgtt gttgcagatg
     9721 ttgtcgctgt tccagctcag gcggcagatc ctcgtaattc tagaggagga gacaatcgtc
     9781 gtggtcctgg tggtccaaga agagacaatc gtcgtggtcc tcgccgcgat actcgtccta
     9841 agtccgagtt cgaccagaag atcgttagtc tccgtcgtgt aacccgtgtg gttgccggag
     9901 gccgcagatt cgctttctca gtttgtgtcg tagcaggcaa caggaagggt atggtcggcg
     9961 ttggtcaagg taaggctacc gatactccac tagcaattga gaaggcgttt agagatgcga
    10021 agaagaacat gatctcaatc aacatgacga agtccatgtc tatccctcac gaggttgagg
    10081 ccaagtatgc agcatcgaga gtgaagatta tgccagcacc aggtaagggt attctcgctg
    10141 gttcatcagt gcgtgcagtg ctcgagctcg caggtcttaa ggagatctct tcaaagctcc
    10201 tttctcgttc taagaacact attaacaatg cctatgttgc tatcaaagct cttgagcaac
    10261 tcaagagaac caagataaag gccaaggaca gataacaata ccaccatgca actacacact
    10321 ctcaaatcaa agaccaagag aaagaagtca cgccaagtag gtcgtggtgg taagcgcggt
    10381 aagacctctg gtaagggtac caagggtcag aacgctcgtg ccggtagaaa gaagcgccca
    10441 gagctaagag acttcatcaa gcgttttcct aaacttcgcg gacgcggtgt ctcttcattc
    10501 aaatcattcc aagacaaggc agttgttatc aatttgaagg cgctcgacga ggcatacaag
    10561 gttggtgaga cagtctcagt accaactcta cttgagaaga agcttatcga catgaagaac
    10621 ggcaaggcta ccaaggtcaa ggttctagca acaggtgact tgagtaagaa actcgatttc
    10681 gaggggctat tggtctcaga gagtgcaaag agtaagatag agaaggcagg cggcactatc
    10741 cgctaatcaa atgtttaaaa cttttataga caaggtaggc ctagtactaa gtgactcggt
    10801 ccttaagaga agaattttaa ttactattgg tattctcgtg cttgcgagag tcttggcagt
    10861 tgtcgcaata cctggtatcg accagagtgc gttggcatcc ttcttctccg gcaaccaatt
    10921 cctctctctc ctcaacatct tctcaggagg aggtcttgca tctctctcta ttatcatgct
    10981 cggagtcggt ccttatatca ccgcttctat tatcatgcag cttttgacct tgatgattcc
    11041 taatcttaag gatatgcagc aagacggagg ggactcaggc agacaaaagc tttctcaata
    11101 cggtcgtctc ctctctgttc ctctatcatt cctacaggca tatgctttta tctctctctt
    11161 tcagaagcag ggagtactac cagcactctc tacaggtact cttgtactag acattttact
    11221 aatcaccgcg ggctcgatgc tcttagtgtg gttgggagag ctcattactg agtttggaat
    11281 aggcaacggt atttcgctca tcatttttgc aggtatcgta agctccttgc cagctgctat
    11341 tgcgcaatta gcattcacct tcgacccatc acagattcct gtttatatcg ctatgtttgt
    11401 cgtaaccatc ctgcttatct tcggagtggt tattgtgagc gaagccgagc gtccaatacc
    11461 tataacttat tcaaagcaga tcagaggtgg tgcgacatcg ggtggtatgc agagttatct
    11521 accacttagg gttaaccaag caggggtaat cccaatcatt ttcgcactct ccatccttct
    11581 tttcccacag atgattttgc aattccttac ttcttctact gtagcttcag tagcaagcgc
    11641 ggctacatct attctcgctc ttcttaataa tctttggttc tataattcag cttacttcct
    11701 tttggtcttc ctcttcactt acttctatac agcagtgacc ttcgacccag agcagattgc
    11761 agagaacttg cagaagtctg gtgctttcgt gccaggtatc cgcccaggcg agccaacagc
    11821 ggagtacatc tcaagagtat tgactcgtct tacacttatc ggggcgctct tccttggtgt
    11881 ggtagccgtt cttccacttg tgcttcgttc ggtgacacag atccagtcta tggcgattgg
    11941 tggtacagca cttcttatcg tggtttcagt ggtaattgat ctcatcaaga agatcgacgc
    12001 acaactctca gtacgagaat actagttttg ctctataatt agagcatgtt acatacagtt
    12061 gtatttatag gtcgcagtgg ttgtggtaag gggactcagg ccgagttgct tcgtaaccgt
    12121 atcaaggagc acgatttaaa gaagcgacag atcctctata tcgagacagg cgacggattt
    12181 agagagttcg ttagaggtaa gagtttgtcg tctaggttgt ctaaggctat atatgaccgc
    12241 gatgctagac agccagactt ccttgctact atgatgtggg ggaagatgct tctcgagcag
    12301 ttggatgagg agatgcacct agtcttcgac ggtgtagcta ggtccaaggc cgaggcacat
    12361 atgctctcta ctgcactctg gttctatgag agggagaagc caacaatcat ttatattaac
    12421 gtttctcgca agtggagcga ggagaagctc ttgaagcgcg gacgggccga tgatgtaaac
    12481 ttggcgagaa tagacaagag actcgactgg ttcgacaagg atgttatacc ctcgattgag
    12541 tacttcaagg agaatccatt cttcagagtg atagaggtga atggcgagca gagtgttgag
    12601 gcagtgcatg cagaattaat ttctaaatac gaatactcag agtgatcaga ctaaagacca
    12661 aagaccaaat acaaacaatg catgagggtg gtaagcgcca ctctgagatt ctccgtgctt
    12721 tagccgagat ggttactccc ggtatgtcca ccttaattct cgaagaggaa gcacgaagac
    12781 ttattcgtgc tcatggtgac acaccagctt ttttaaacta ctcacccaag ggtgcgtcgc
    12841 gtcctttccc cgcagcactt tgcgtatcta taaacgacga gatcgtgcat ggtattccta
    12901 atgagaagga gaggatactc aaagacggcg acattgtctc tctcgacctt ggactaaagc
    12961 acgagggatt cattactgac tctgcggtta cagtaccagt cggttctgtt gatgatgagt
    13021 ctagagacat catgcgttta gccaaggaag cactagaggc ggggatagag gctgcaaagc
    13081 cgggtgcgca cataggcgac ataggcgagg cagtgtacaa ggtagttaag ggtactggtt
    13141 tttcccttgc cgaggaccta gctgggcatg gtgtgggctt cgaggtgcat gaggatcctt
    13201 ttgtaccgaa cattggtcgt aagggagagg gggagaagct ggtaccaggt atggtcatag
    13261 ccatagagcc gatggttaat gctggaggta gtgctatagc cgacacagat gacggctaca
    13321 ctatagtgac agctgacgga tctcgcagtg ctcatttcga acatactatt gctattaccg
    13381 agacaggaaa tattgttttg actcgttagg tgggtgtggt aaggtgggcc ttagaacgaa
    13441 cctataagga ggtcaaatgt tcgctaagat cttgcttgga ctaatcatgg ctatcgtagc
    13501 tctggggttc ctggctgagt ggtactacca ctggatcagg cgtgtgcagc cagtcgacca
    13561 ctctgatcca ttcgcagtgc cagatctcga taaatatgca gtagaggata gttcaccaac
    13621 accaagagcc gaggctttct ttagggcgaa caacgaagct gctagtgaga tgggaatgac
    13681 actagctggg tactctgctc ttatcatcaa gagtgcagcc gagtctccgt acccaacaaa
    13741 caaatgtctc acagaaagtg agcttgagaa tgtggcaaag caaaggtttg gtcttatttc
    13801 tccgagagag gctgatcatg catatcattg caagtattgt cgatcgggag tgttgtggta
    13861 tcgcagtcgc ggaccgagat aatctcggtc tttttctttg gtctaacctt tgttatactc
    13921 atcttctaat tcatatttat gtcacatcca aagaaagaaa ggagtttcgt agcagttaag
    13981 catgacggag tacagaggac tctggtgggg gagatcatca accgagtcga gagaacaggt
    14041 cttaagatca taggtctcaa gatgtttgta cctagtatgc ctcagacagt agagcattat
    14101 ggtaaggatg atgcttggtg tgagaaggtt gggcagagga ctatagacaa catggccaag
    14161 gagggtaaaa caaccgacaa gacggcagtc gagattggtc gcgaggtgtt gaatgctctt
    14221 tataaatact ttacccttgg tccagtcgtt ggatttgtcg tagagggtaa caaagctgtc
    14281 gatattgtga agaagttggt cggtggcacc gagccaacca cctcagatgt gggtacaatt
    14341 cgaggcgatt acaccctaga ctcttatgat ctagccaatg ttgatggccg agctgttaga
    14401 aacttgatac attgctctga caagccagag gaagcagagc gcgagataaa aatttggttc
    14461 aaaaacgaag aattgatcaa ctacaaatct attcaagaag cgatattgta cgacgtaaac
    14521 attgacggca tcttagaata attccgtatt cgagtttaac ttacctcata ttttggaaat
    14581 ctggggatta ctccctactt acaacttttg caaaagttgt aagtggttat aatggttgaa
    14641 gggttatcta aggtattatc tagctaacca atatttaggg atctcgaatc gtttgagacc
    14701 ttggtcccaa actgagagaa cccacctagt tttagaacta gggaataccg agataacccc
    14761 atgaaagtcc cccattccaa gtatcggaat ggggggttct cgtttataat atgccgatgt
    14821 cgagatcgat actgatgtcc gccctctttt tctctgtatt ggttggagtt ttgaacaaaa
    14881 ttggtggcat atacttcttg tattggggtc tttcttggtt cgatatgtta atgcatttct
    14941 tgggaggggt cactttggcc ctatttttta tctggggata ttattacagc ggattcctat
    15001 caaggaatgt cacatcagac aaattatatt tttttaaaat tatttgttta gtcatgttgg
    15061 tggggttggg gtgggaggtg tttgagtttt atttcgatat agcgcaccct acaagaggca
    15121 agtactacga agatacattt ttagatttgt tttttgattt tttgggagta tggtctgtga
    15181 gtttgcttat aaaatttaag gagaaaaaaa tatgaagacg agtctaactt gctacggtgg
    15241 tgtgggcacg gtaactggag caaacttcct cctcgaagtt gagagtgggg gcaagatact
    15301 tcgtgtgctt atcgactgtg gactcttcca agggttgata gccgaagacg atgtgtcgaa
    15361 gaccttcgat tatgatgtga caaccataga ccatctcttt atcactcatg cacatattga
    15421 tcacattggt cgcattccag atcttattaa ggcaggattt aagggcaaga tcctctcaac
    15481 tcgagagaca aaggacattg cccgctttat gttgaaagat ttgtcgcaca tagcggggga
    15541 gggagacgat gctgccgaga aggctctcag tatgtgggag atacatccat accataccaa
    15601 aaagttgttc gagcttggtg acgaagaagt tttctcgctt gagttattcg acgcggggca
    15661 catcttaggc tctgcgatgt acaagctcac attcccctcc ggcaagtcga tactttttag
    15721 cggtgacatt ggtaactcgc cagcaccact acagagcgat gtcgagagag tgagcgatgc
    15781 cacttacatg cttatggact ctgtctacgg agacaggaat cacgagagta tagaggagag
    15841 agacaagaga ttcgctgaga ttatagccga ggttatagag aggaagagca ccctcctcat
    15901 accagtcttc tcgctcgagc gtacacaggt gatgatccac gagttagaca agctcttcac
    15961 ttctaaggga cttaagagca tccctgtttt cctcgactca ccacttgcta tcaatatcac
    16021 cgagatttat agagacagat ttaatagtaa ggataaggac ctctacttca acaaacttca
    16081 agagactgca caggtgcgag actctattag catcagtcat gtccctggac caaagataat
    16141 tatggcgggc tctggcatgt cgactgcagg acgcatcgtg catcatgagg agaggtatct
    16201 acctgacccg aacgccacta tactctttgt tggttaccag gcagcaggta cactcggcag
    16261 actcatccaa gatggtatca aggaagtggt gatcaatgat aagaaaatta aggtgaatgc
    16321 gagggtgctg agtattggag gcttctccgc tcatgcagac tcagaccagt tggtgaagtt
    16381 tgtcgaagcg agcgagaaaa ctctggagaa agtatttata acaatgggtg agccgaagtc
    16441 atctattttc ctagcacaaa gactcaagga cgaactcgga gttgactctg tcatcccaga
    16501 gaaggggaag gtgtacgaac tagatttata aataaaaaaa cccgggacgt cccgggtttt
    16561 tttatttaac tgcttctacg atcttcacga aaacggttgg atgattctct gcgaggtcag
    16621 caaggatctt acgatcaagc ttgataccct tcttgttaag agcgtcgatg aacttggagt
    16681 aggagagacc aagtggctta acagctgaag agatgcggac gttccaaaga cggcggaagt
    16741 cacccttctt gtctcttcta tgagcgaagg cgtatgtacc cgcatggaag atagcttcat
    16801 tagcctgacg ttctttggta gagcgaccaa agcggtatcc cttaactttc cttaatatat
    16861 tctttctggt tttgagggca tttacaccct ttttgactct ggtcatctta ttatttgata
    16921 tttactagga aacggcttct ctccttattg ctcatgtgga attcgcccaa cttgcccttg
    16981 gcaaccttct ctgagctaga ctccttggca ttgaagtggt tttgaccagt tttgcgcacc
    17041 aaaatcttgc cctttttggt gactttgatc cttttggcga acgatttgtt ggtcttaatt
    17101 gacataaaat aagccgttta ttggcctgta catattactt atttctagag gtttgtctag
    17161 gctttctcga taatagcggt catgcccttg gggcttttct tgggggcgtc ggcgatcttg
    17221 tgggggatgg tgactagctt gagcaggcgc tcgatgcgtt ccttgaggaa cttctcatct
    17281 aggtacttgg cacggccagg gaggaagagg ttgatctgga cacggttacc ctctgcgagc
    17341 cattccgagg ccttcttggc ctttaggccg aggtcgttgt cgccggtacc gagctttacc
    17401 tggatacttt taacctctac agagtgagcc ttctttttgc tctccttctc cttcttggct
    17461 tctgcataca tgtacttgcc caggtcagtg atcttggcaa ctggagggtt agcatttgga
    17521 gagatctcga caaggtctag gtcgaattcc ttggccttct tgagtgcttc gtccaagtta
    17581 agaacaccga agttctctcc ctcagggcct atgactcgaa cttctttaga ctttatagcg
    17641 tgattcgttc tcggggtgag caatgccttt ttattagttg gtgtgagtat acactatttc
    17701 accctaggtt tctactggta ctatataggt atggaaataa agcacaccct gccagacctc
    17761 gagcaaatta tccgcgccag acatccgctt aagaactctt tcgtcaagac tcaagaaaca
    17821 ctttcgagtg ccgagcgctt tgctgtctat gttacgcatc atgtgggttc ttttaggttt
    17881 ttcactattc tattgatttg gagttttctg tggttggctt ggaacctttt tggccccgtg
    17941 gagctacgat tcgatccagc accagccttt gttatatggc ttttcctttc aaacattatt
    18001 caacttatac tcctaccact catccttatt gggcaaaact tggagaaccg tcacaccaac
    18061 atgcaagcag aggaggatta tcaggtgagc aagcattctg aggaggagat caagatcatc
    18121 atcggacact tagagaatca gaacgagctt atgctagaga tcatgcgcaa gctagacaga
    18181 aattaggagt ggcactagaa tggttgatat gacaattaag cagacattta ttggactcga
    18241 tctggcctat catgtgggtt cggagtctta tgtctgtctt tcttaagaaa ataaattaag
    18301 gaaaccaaac acaagcctcc gaatctcgga ggctttgttc tttcatccag tgtctcactc
    18361 ccaccaagag gaccagatgc aacatgagct cgaagtcgta gacgttgtcg agaagaatgg
    18421 caagaaggtc gccaagatca agcatcagtg cgacccgact agactcatca cgctcaccga
    18481 gacagctgtt ggttgggtgg acgaacttgg ggtgaagtat ccagccgact acattgaggt
    18541 gtagtcttat gtcccgcgca tagcgggact ttttctatag aaaaaacgca ccacaagtgg
    18601 tgcggactta cttcaggaac ctgatcccaa ttttgataag tatagcgagg aagctaagag
    18661 tgcacatcac accggtagta cggtggaaga agctctcaag tatcgagtcg aacatcggta
    18721 cttgcttgtt cttcttccgc ttcgcatcat ttttctccga ctggatacag tagagaagaa
    18781 ggggcaagaa tgttacaagg atcatgacag cccagaggtt gtcgaaacca agcaccaagt
    18841 cgagcacgct cactcctttc taagggttag gctctgggat tttcttgatc ttgattctga
    18901 actgtttggg tccgccagta gccatcttgt ggagagctct gtggcaaata ccaatcattg
    18961 taccttcgaa gcaaatccat ttgccggacg ttgcctcaag gttatcgagc ggatgaggta
    19021 tttcgaccag tgtgtatgta ccaggtggac actccatctc aacaaaattg ttgcttgcat
    19081 cgtagagata gaatctcacc gggaaggtga ctttgtacat gttcgccacc tccatttgag
    19141 gttcaggtct agagcgatac taagtccaat aaacctcaaa gtcaaacaaa taaaaagcgc
    19201 agatcttttg atctgcgcag tgtaatacgt ctgctctaca tcactcgggc agggtcgatt
    19261 cctcgatctg cttcatcatc ttcgtctcgt cttccttgcc gatatagccc agttcgactc
    19321 cctcgctcac agtcctcttg gcagtcgact tggtgctgaa ttttcgctgc cagcgctctt
    19381 cgccattgac gaagagtgtc gctcttccgt cggcaatcat catgtcgtag gttccggctg
    19441 gagctgtcac agttggttcg aggataccca gaagcatcat ctcgagcatg ctagtaggct
    19501 tcggtattcc gtcatggggg tgctcactcg tctccggtag ttccgagaga gcgatatcct
    19561 ccgcgatcgc cgacttctgg tccttaggga cccnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnagttccg agagagcgat atcctccgcg atcgccgact tctggtcctt
    19741 agggacccgt ccgtcagcca ccacctcctc caggagctcg agcgctcgac ccttcgagta
    19801 gaaggtgggg ctcgccttat cgccagttgg caggtggaag tacccatgtt gtacattggg
    19861 gttgcacccg cacatctgga agctgaagcg tccatcggtt gaccggtgtt ccgtcttggg
    19921 ctctgctctt gcggtcatgc agcgtttcct cttggttggg gaggtgaagt ggggactact
    19981 ataaggatct gtttgatagc acgatagtac atttatgtct aaatgtcaaa tatgaaaaca
    20041 gtattaaaaa agccctgacg aatcagggcc agaatcagta cggtaacatc tagtgagcga
    20101 tgatatcgtg gtcccaacct tctatcgaca ctccccggat gtcgtcgagt gccacgaagg
    20161 tgtgaatacg ggcgtcgaca gttccaggtt tgcggtatga gatctcgact gcttcatcac
    20221 tgacagcatc tactctgcct ttgacaatag aggtgtcgcg gcggtcgaca gagacatcct
    20281 cgcccttctt ttcttcgaga tgcttcttgt acgctagagt actcacaggg tcctcggctg
    20341 gttggtggat acaaaatctt ttttgataat ataatactat tatataaatg tcaaatatat
    20401 gaagacatga aaaactccac catgagtgga gtgtaatact agtcagaata cggaggtgtc
    20461 atgagcttga actcgaggag ttgtccgaaa atgaatttga tcagatattc ggctgcatca
    20521 tcatggtgca tctctaggag ttcctcggct ctatcgagga aggtgagcag gtgtccgact
    20581 cggaactctc cattgtttgc tgtgattaag ggttcgttct cataacccca acagcggaga
    20641 gctccacggt atttcctgat catcattcgg aacatggctt gccggtcgct tcttcgcatg
    20701 tcagctctga gacgcctacc gacatcacta aagtgtccaa agataccaaa tttggtaatc
    20761 tgctcgtcat cccaactcgg ggttgtacca cggtttccca taaactcctc ctgtgaggtt
    20821 caagattctt caaccagcct actctcaaaa ttaaaaaatg caaaagaaaa actccgacac
    20881 tgtcg
//
LOCUS       DAORIT010000007        20338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_424_length_20338_cov_52.138047, whole genome shotgun sequence.
ACCESSION   DAORIT010000007 DAORIT010000000
VERSION     DAORIT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20338
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_424_length_20338_cov_52.138047"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLD99_01045"
     CDS             complement(<1..791)
                     /locus_tag="PLD99_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25528.1"
                     /translation="MKKRKISLSVTVGLVITLVASFLGAFSTPVLAQGAPSIISYQGR
                     LADSGGNLLGSTSGTTYYFKFSIWDNQTVGSGSRVWPVALPGVTTSTVRSGVFNVNIG
                     DTTNGYPDALDYAFSNPNIYLQVEVSADNSTYETLSPRQRLSSTIFAQVANSVLGASS
                     TITTLTNTNIASTNGTTTNATSTNLFVSSLASTTQLRANIASLGNLTSVSSIITNLAG
                     FGSRCVQTDNNGQLSVAAGACGTGGSGSGGGSWATTTSQTSSYIN"
     gene            complement(836..1396)
                     /locus_tag="PLD99_01050"
     CDS             complement(836..1396)
                     /locus_tag="PLD99_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRH25529.1"
                     /translation="MVTFDRKKIKRRIKPSKNFFLSLAGSIIYQQISTKAGDAIYARF
                     IKLFGRKKPTPDNFLSISEVNLRSVGLSTQKISYLVDLASKFIDKTIDPKLIPKMRDD
                     DVKEHLIRVKGIGPWTADMFLIFALNRPNILPVGDLGIKKGFKKVFGLKMLPSEKKMR
                     ILAKSNEGEHTYLSLYLWESINGDKN"
     gene            complement(1396..1483)
                     /locus_tag="PLD99_01055"
     tRNA            complement(1396..1483)
                     /locus_tag="PLD99_01055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1447..1449),aa:Ser,seq:gga)
     gene            complement(1526..1957)
                     /locus_tag="PLD99_01060"
     CDS             complement(1526..1957)
                     /locus_tag="PLD99_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25530.1"
                     /translation="MTNIQKVSILALRVTMGWMFFWAGITKILDPNWSASGYLSGAKA
                     FNQLFEYLSTPNILPIVNFVNEWGLALLGVSLILGVLVNVSIPLGILLMVLYYLPLGF
                     PEPNAHSYIVDEHIIYIFSLVLLWVLGAGNVWSVSRIWRSR"
     gene            complement(1977..2867)
                     /locus_tag="PLD99_01065"
     CDS             complement(1977..2867)
                     /locus_tag="PLD99_01065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRH25531.1"
                     /translation="MTSNIYAQADKNIRKTWLLMALFLVVVIGIGYTFSCIYGDSNIL
                     YIAVIFAVLMNVISYWYSDKIVLKLHKAIPVTRDGQRDLYNSLENLAITAGLPMPRLY
                     IIEDAAPNAFATGRDKDHAVVCVTTGLLHLLNKNELEGVLAHELSHIGNRDILISTVV
                     VVLAGFITILSDIFLRSTMHNSRDRDSRAGGMMFVLGLILAILAPIAATLIQLAISRK
                     REFLADASGALLTRFPEGLASALRKINTNSISMKTANTATAHMFIANPFARESKSFSF
                     VRNMFLTHPPLEDRVKALVG"
     gene            complement(2873..3424)
                     /locus_tag="PLD99_01070"
     CDS             complement(2873..3424)
                     /locus_tag="PLD99_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRH25532.1"
                     /translation="MMYIWIILGVLVLWGVFSYNGFVKLVNRAEEAWSDIEVQLKRRY
                     DLIPNLVNTVKGYALHESTAFEKVTEARSRAMQAGNREDKGKAEMELSGSLKSLFAIA
                     EAYPDLKANQNFLSLQGELSDTENKIQASRRFYNTNVRDLNTSVDSFPGNLIAKMFNF
                     AKKEFFELAEDESVAKNPVQVKF"
     gene            complement(3447..5105)
                     /gene="groL"
                     /locus_tag="PLD99_01075"
     CDS             complement(3447..5105)
                     /gene="groL"
                     /locus_tag="PLD99_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411268.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRH25533.1"
                     /translation="MAKQIIWGDEARKELKSGVDQVANAVRITIGPRGRNVVIDRGYG
                     APMITNDGVTIAKDITLPNKFQNMGAEIVKEVAQKTNDLAGDGTTTSVVITQALVEAG
                     FKKTMMGANPMGIRRGIEAATRDAVAHLKKMSKPIKTGEEIKQVATISAESEEIGVII
                     ANTIKKVGKDGVVTVEASQSFGIESEVVDGFEFDKGYISPYMITNTERMEAEYKDPLV
                     LVTDKKISSIKDVLPLLEKIAQSGKKELVIVADDVDGEALTTFVLNKLRGGFNILAVK
                     APGFGDKKKDMLQDIAIMVGAKFVSEEVGLTFEKLDIGHLGRAQKIVSTKDDTIIVGG
                     KGKKADILERVKNLQSQRENSKNKFDREKLDERIGKLTNGVAVIRVGAATETEMKYLK
                     DKIEDAVNATKAAISEGIVIGGGSALVKVSKFLEEKFRLSKEAKTAHESVLSSEYSAG
                     YLALIESLRMPLKQIAINAGMDGGVVVSEVMKGGENFGYDALNDEFKENMFEEGIIDP
                     AKVERCAIENASSAVAVLLTTEVAITEEPKKESKSDLGGSDMSY"
     gene            complement(5110..5427)
                     /locus_tag="PLD99_01080"
     CDS             complement(5110..5427)
                     /locus_tag="PLD99_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRH25534.1"
                     /translation="MKKVIKKTKIMPVGDRVLIRPMGNDLPSSIGKIKIVLPESVTSE
                     KSDRGLVLAVGEGRYQDGKLIPIKLKVGDMVIFSKYSYDEVKQGEEDLYLVKSENILA
                     LIK"
     gene            5502..5726
                     /gene="secG"
                     /locus_tag="PLD99_01085"
     CDS             5502..5726
                     /gene="secG"
                     /locus_tag="PLD99_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRH25535.1"
                     /translation="MTNILPYIQAVLSVVLVVAILLQRSDASLGSAFGNEQGNTKFTR
                     RGFEKFLFNATIVIAILFAVSAILSLFYRS"
     gene            5739..7493
                     /locus_tag="PLD99_01090"
     CDS             5739..7493
                     /locus_tag="PLD99_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRH25536.1"
                     /translation="MENKNFRALLSKRFHIPKARTIERALTRFTSAEKALFYSLTFLL
                     VLTSIIMLLKVNSAFLVRVPISGGSVTEGVVGNPRFVNPVLAISEADKSLVSLIYSGL
                     VRIGVNGDTRNDLAESIVVSEDGLTYEVTLRKDAVFHDGRPLTADDVVFTIQKVLDPN
                     IKSPQFGDFAGVTISKVDDHKISFSLRKPYSPFIKNLTIGILPKHIWSTVTDDEFSFS
                     QWNVLPIGSGPYMVDKTDRDSGGIPNYYNLIPFDEYLYDKPFISNYIFKFYSSETDML
                     EAYDGGALDILGGISPDEALTINKNRSDIINSPLPRIFGVFFNQNANKVLLDKTVREA
                     LDIAAPKEEILKIVLNGFGRIIDGPLPPMFSKASTTESSKTAAERLELASSTLSKAGW
                     VKNKDTGILEKKNKSDTIKLSFSISTSDNPDLEKVAQMLEAAWTALGAEVKVSIYESG
                     DLNQNVIRPRKYETLLFGEVIGKDGDAFPFWHSSERNDPGLNIALYVNNQVDKILESI
                     RSERDEDKKNDLYLSFEKAIKGDIPAVFLYSPDYIYVIPEKLKGISLEKLSKPEDRYR
                     GTTGWYVETNSVWKVFQK"
     gene            7526..>9297
                     /locus_tag="PLD99_01095"
     CDS             7526..>9297
                     /locus_tag="PLD99_01095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRH25537.1"
                     /translation="MNNQSNFKRGLRGKNMHRRNDRPIAEKRAPDILPPLAPGNIRIV
                     PLGGVEEIGRNMMAIEYGNDIIVVDCGIQFTDAETPGVDYILPNVKYLEERKGNIRAM
                     VITHGHLDHIGGIPYIMNRIGNPPIYTREFGALLIKKRQEEFPHMPPLDLRIVDQSDK
                     SIAITPNLKVRFFGLTHSIPDSTGVIIETPLGDIVNTGDVRVDNENGVPTDKEVEQYK
                     MFKDRNVLLFTMDSTGIEKPGFSPSEDFILKNIDSIVANVPGRVIIATFASQVERIIE
                     FMRIAKRYGKKVVIEGRSMRNNVDIIKHLNLVELDHIVPIEDIEKYPPNKIMMIATGA
                     QGEEFAALMRMSNKTHKFIKLQRSDTVILSSSVIPGNERAIAKLKDNLFRHDAKIITY
                     LDSDVHTSGHGKRGELEWVHKQIPYKFFMPVHGHHYMLKMHAELARQLGAAPENVIVA
                     DNGSLIEIREEGKIITRAVEKAPSEDMVVEGFSVGDVSEVLIRDRKMLAEEGFVVVVA
                     MVVTKTGKLRKSPDIISRGFIYLRDNQELLTETRHIIKRAIESTTVGMNPINFDYAKN
                     AVSEDVARFLFQKTMKKPVIIPVIL"
     assembly_gap    9298..9397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9398..10519
                     /locus_tag="PLD99_01100"
     CDS             <9398..10519
                     /locus_tag="PLD99_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRH25538.1"
                     /translation="IFVANFFFSIHFAATLYINSSFLGHFFSPSTIGWLYLLGALGNV
                     YFLMNAERMLSYLGSKGFLLFFSVTTLISTIGAALATTPGMATIAFLIYASVSMMIYY
                     SFDILLEEVSKNENTGKTRGIYLTITNAAIMGGPVLIAFAGDQNNFKDLYILSAYFLI
                     PIFLLMIFSLKTKRGVETYLKVPLPFSVWKELKNVRRVTLARSVLEFFFAVMVIYMPI
                     YLHNIVGFSWEQIGIIFTFMLLPFVLFEWPAGELADKKYGEKEIMFSGFVILCLSVLI
                     MPYLGSSMIIWAIALFVSRIGASLIEITTDSYFFKQVGPHHVGLISIFRLSRPVGLML
                     GAGIGALSNAFLHQSSIFLILGVMILYGIRQSFLLVDTK"
     gene            complement(10525..11121)
                     /locus_tag="PLD99_01105"
     CDS             complement(10525..11121)
                     /locus_tag="PLD99_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25539.1"
                     /translation="MKDVPKALRVLGKLNTPDKIQNFLNNIPFNHERKGETYHSVLKT
                     LESGKAHCFEGALVACAALWLQGDKPLLMDLRTAKGDQDHVVALYKVGALWGAISKTN
                     HPVLRFRDAVYKTPRELAMSYFHEYYLADGTKTLRKYSAPFDLSKSKFDWLLGDDDLS
                     QLVNALDNSPHTDIVPKELKLRKADEIERTFTDHVEYK"
     gene            complement(11123..11674)
                     /locus_tag="PLD99_01110"
     CDS             complement(11123..11674)
                     /locus_tag="PLD99_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HRH25540.1"
                     /translation="MHPQGVNDTSDTKIWMVLIGIIVFFVLIIIYAQHITNVRRGEPV
                     PVVARYTNYVYGVNLRYPPEWQPVGGLSFDRYEGESGFFSISAGGEDNVSIDTLTQSE
                     AKNNKKPYGSEPYIQTLTIDGQDARLILPSLDQSPAMHGQAVLIVRYPTPKVVGSTTY
                     KYLVFWADRANIQDIASSLTFIK"
     gene            complement(11694..12767)
                     /locus_tag="PLD99_01115"
     CDS             complement(11694..12767)
                     /locus_tag="PLD99_01115"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HRH25541.1"
                     /translation="MLWSKLNSIYKNYEYHISSIALVLGFIFDVLTLRRADAFFENFW
                     VVFHLIVVATAILIINRRSKDRDADSDTDFWLLSMMQFSFGGLLSAFLILYFRSSSLA
                     VSWPFLLMLALAFVANERLKRNYQRLTYQVSFFFSALLLFSIFFVPVVVGYIGPTIFV
                     FSGLISLLVLRFFLAILQRVSGEKFTLALGSIRGIVLAIFALVNILYFTNIIPPIPLS
                     LREGGVYHLVDKEITGQYVLRAERKSWLEKLSPRTSVNILPDQPLFVYTAIFSPAKFS
                     TKIVHEWQYFDQGKDEWVSVSRVLLPITGGRNNGYRTYSISSVVPGSWRVNVMTERGQ
                     VLGRIWFSVDYVGAQPELVTEVH"
     gene            complement(12826..13224)
                     /locus_tag="PLD99_01120"
     CDS             complement(12826..13224)
                     /locus_tag="PLD99_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRH25542.1"
                     /translation="MKGYITNIEKETLENNNFRKVLYTAKSSQLVLMSLEPLQDIGEE
                     VHQLDQFLRVEEGIGRVVLDGVGHEVRDGSAIVVPAGTKHNLINTSVDKPLKLYSVYS
                     PPNHKDGTVHETKADAEANEEHFDGVVTEA"
     gene            complement(13244..13609)
                     /locus_tag="PLD99_01125"
     CDS             complement(13244..13609)
                     /locus_tag="PLD99_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25543.1"
                     /translation="MKKVFIEIFFSLAFVAVATFFVYGQLVGVNDFWSAQNVWSIVLA
                     CGWIIVSLGYYHQGYVVHKSKSAEHVSVVLPGAVFIVQCILFVKGIYYDDWALIFGAL
                     VVNSGVVFSLYQITHVKFR"
     gene            complement(13606..14004)
                     /locus_tag="PLD99_01130"
     CDS             complement(13606..14004)
                     /locus_tag="PLD99_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRH25544.1"
                     /translation="MLIKLYIITLPVLLAIDLVWLRFVAKGFYQDKIGYLMKPEVNLV
                     PALIFYLLFALALVIFVISPALSEGVWTKALLLGLFLGFISYAAYDLTNHATIRDWPL
                     MMTVVDMLWGALLSGATSVIAYFIARKIGL"
     assembly_gap    14035..14134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14135..14460)
                     /locus_tag="PLD99_01135"
     CDS             complement(<14135..14460)
                     /locus_tag="PLD99_01135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HRH25545.1"
                     /translation="MSLKLETPVTKFKLRTGRGLSGKGVFALETIPKGKRIIEYIGRN
                     IPIEEQEFATGRYLFSTGRNKMINGNIKENVARFINHSCKPNCEARGPSGRVFVYSLR
                     RIKAGE"
     gene            complement(14504..14574)
                     /locus_tag="PLD99_01140"
     tRNA            complement(14504..14574)
                     /locus_tag="PLD99_01140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14540..14542),aa:Gln,seq:ctg)
     gene            14686..14961
                     /locus_tag="PLD99_01145"
     CDS             14686..14961
                     /locus_tag="PLD99_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25546.1"
                     /translation="MLVVPLPIRSLIEEHRRNGTARPGRAPMMTTSQKGDVKRYGEDL
                     KAEIARLDMELRGLKQQQLDLSSICDHERQTNGKCKHCDQPVLAIMA"
     gene            complement(14997..15419)
                     /locus_tag="PLD99_01150"
     CDS             complement(14997..15419)
                     /locus_tag="PLD99_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HRH25547.1"
                     /translation="MNKIIALLGVAVVLVGGFLLMKTGQGGTLLDSVGNAVSPVGNTT
                     ENKVSVKEFTVEGSSFAFAPKTMTVNKGDTVKITFINKVGFHDFVIDEFVGAKTKQIK
                     AGENEVITFVADKSGTFEYYCSVGTHRQQGMVGSLTVI"
     gene            complement(15460..16386)
                     /locus_tag="PLD99_01155"
     CDS             complement(15460..16386)
                     /locus_tag="PLD99_01155"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRH25548.1"
                     /translation="MLWTNFKRISKTGFMNFWRNGTVSLASVFMMTATLSVIGFTMLG
                     GAVLTTALTTLKDKVDISVTFIPSAPEEDILKIKSSLETLSEVSLVTYTTREEALEAF
                     KLRHENDQSILTALNELNENPLGAALDIKAKEPSQYKSIAEFLEGNSALSSGGLMIVD
                     HVNYYQNKVAIDKLTGIVRSADKLGVAIALSFILLSVLIAFNTIRLTIYISREEINVM
                     RLVGASTNYIEGPFVTLGVIYAVVATILTALIFLPVTYWIGGVTQDFFIGLNLFSYYI
                     GHFVSIFFTLLGAGVAVGVVSSLLAIRRYLRV"
     gene            complement(16403..17083)
                     /gene="ftsE"
                     /locus_tag="PLD99_01160"
     CDS             complement(16403..17083)
                     /gene="ftsE"
                     /locus_tag="PLD99_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRH25549.1"
                     /translation="MIFFDRVTKVYPDTSVALQNITLTIEPNEFVSIVGQSGAGKTTL
                     LKMLLAEEKPSEGKVFYESSDVHRLSKNEMNNLRRKIGMIFQDFRLISNKTVYENIAF
                     TMEASGKTDEEIEQDVPHVLELVDLANKVWNFPHELSGGEKQRVAIARALVNQPDVLI
                     ADEPTGNLDPVNTYDIVQILKKINDFGTTVILTTHNKGIIDMLGKRVVAMEKGKIVRD
                     DKEGKYFI"
     gene            complement(17144..17623)
                     /locus_tag="PLD99_01165"
     CDS             complement(17144..17623)
                     /locus_tag="PLD99_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25550.1"
                     /translation="MKRYRVLYAYLWQDKETDKYYFEAKHWAPCNGAVVFHRSLIHHS
                     FDKDDGVVVVLGSRDKPYMSVNGHKIRPRQIVEPTYHMLLAKEVFREKQARYCAVCGD
                     KATYTIEGLSHFGSGITGFDRGKIYYSCDKPNHQRWIIGSIEADTISDGPLPEDEVW"
     gene            complement(17716..>18599)
                     /locus_tag="PLD99_01170"
     CDS             complement(17716..>18599)
                     /locus_tag="PLD99_01170"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRH25551.1"
                     /translation="YMASKDEIQKKIDALEERMQDANFWNDKDSAQGAIKELQNLKDE
                     LEGVGKYDKGDAVMTIFSGAGGDDAEDFTNILLQMYLKYFGNKGWSYSVMHKNDNDHG
                     GYRNITLEISGKGVYGTLKNESGVHRLVRISPFNAKKLRHTSFSMVEVIPKFEKVGEV
                     SIPPEDIRLEYSKSSGPGGQNVNKRETAVRVVHIPTNLAVHVESERSQAQNKERALAM
                     LEAKLYKLQDEERKAKEKGMYVSKTTSIEWGSQIRSYVLHPYKMVKDHRTDVEVRDVD
                     RVLNGDLDEFIEAEKGL"
     assembly_gap    18600..18699
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18702..18774)
                     /locus_tag="PLD99_01175"
     tRNA            complement(18702..18774)
                     /locus_tag="PLD99_01175"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18739..18741),aa:Thr,seq:cgt)
     gene            complement(18795..18870)
                     /locus_tag="PLD99_01180"
     tRNA            complement(18795..18870)
                     /locus_tag="PLD99_01180"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18835..18837),aa:Thr,seq:ggt)
     gene            complement(18919..19578)
                     /locus_tag="PLD99_01185"
     CDS             complement(18919..19578)
                     /locus_tag="PLD99_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25552.1"
                     /translation="MWKQLTSLILLVLVFTIVAGVAWFVDYYAPINSSDSVVIGDVFP
                     TKEPEEGQITLKIGESGVFRNISIKPTEVVQDSRCPVNVNCIWAGTLKVKVEVYSGLG
                     ASTPTIELGGSITTEAEEIKLVSVSPEKMQGETLANDYKFVFEVTKRAQAPIGAKGCF
                     VAGCSSQICSDKEEMVSTCEFRAEYACYRSAKCERQASGQCGWTDTSELRMCLNNPPA
                     L"
     gene            complement(19591..19950)
                     /locus_tag="PLD99_01190"
     CDS             complement(19591..19950)
                     /locus_tag="PLD99_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25553.1"
                     /translation="MSNSQKAVWVLRIAVAGEFLGHAVFALQGKADWVKWFAKFGVSD
                     PATATTLLMLVGIMDLIVALVVLVKPMKPILLWAVFWGFWTALVRPIVGMPIWDFIER
                     FANWGAPLALYYLIKKE"
     gene            20185..20258
                     /locus_tag="PLD99_01195"
     tRNA            20185..20258
                     /locus_tag="PLD99_01195"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20219..20221,aa:Met,seq:cat)
     gene            20263..20336
                     /locus_tag="PLD99_01200"
     tRNA            20263..20336
                     /locus_tag="PLD99_01200"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20297..20299,aa:Asp,seq:gtc)
BASE COUNT         5729 a         4928 c         3859 g         5522 t
ORIGIN      
        1 tagtttatat aacttgaggt ctgagaagta gttgtggccc aagaacctcc accagaccct
       61 gatccgccag taccacaagc tcccgcagct accgaaagtt gcccgttgtt atctgtctgt
      121 acacatcttg agccgaagcc agcaaggttg gtgattatag acgataccga ggtaagatta
      181 cccaaagaag cgatatttgc tctaagttga gtggttgacg caagactgga tacaaaaaga
      241 tttgttgagg tggcattagt ggttgttcca ttggtgctgg ctatattcgt atttgtgagc
      301 gtggtgatag ttgacgatgc accaagaaca gagtttgcaa cttgtgcaaa aattgtcgat
      361 gaaagtctct gacgtggcga aagtgtttcg taagtactat tgtcagcaga aacttctacc
      421 tgaagataaa tatttggatt actgaaagca tagtcgagcg catcagggta tccgttggtt
      481 gtgtcaccaa tgtttacatt gaatactcca gacctaacag ttgatgtagt aactccagga
      541 agagcaaccg gccaaaccct agaaccagat cctacagttt ggttatccca aatcgagaat
      601 ttaaagtaat aagtggtacc agaagtacta cccaaaaggt tcccaccaga gtcagcaaga
      661 cgaccttggt aagaaataat actcggtgct ccttgagcta aaacaggagt agaaaatgca
      721 cccaaaaagc tggccacaag agtgataacc aatccaaccg taactgacaa tgaaattttc
      781 ctctttttca aaggtttgct tcctcatatt ttaccgcatt taagccattt tttgcctaat
      841 tcttatcccc atttatcgac tcccaaagat agagagaaag ataggtatgc tctccttcgt
      901 ttgatttggc cagaatgcgc atttttttct cacttggaag catttttaat ccaaaaactt
      961 ttttaaaacc cttctttata cccaaatctc ctactggcaa gatatttggc ctgttcaaag
     1021 caaaaataag gaacatgtct gccgtccaag gtcctatacc cttaactcga atcaaatgct
     1081 ccttaacatc atcgtctctc atttttggaa ttaatttcgg atcaatagtt ttatcaataa
     1141 atttacttgc caaatctaca agataagaga ttttttgtgt tgatagacca acagatctca
     1201 aattcacttc agaaatagac aaaaaattat ctggagttgg tttttttctg ccaaaaagtt
     1261 ttataaacct cgcataaatc gcatctccag cttttgtaga aatttgttga taaattattg
     1321 atccagcgag tgaaagaaag aaatttttgg acggttttat ccttcttttt atcttttttc
     1381 gatcaaatgt aaccatggcg gagggtaaga gattcgaact cttggtgagt tgccccacac
     1441 acgctttcca agcgtgcgca ctagaccact atgcgaaccc tccagttcgg ctatagtagc
     1501 tctttttcaa tcttttgaga atatgctatc tagaacgcca aattctactc acactccaca
     1561 cattccccgc tcctaaaacc caaagcaaaa caagagaaaa gatatatatg atgtgctcgt
     1621 ctacgatgta cgaatgagca ttgggctctg ggaagccaag tggtaggtag tataggacca
     1681 ttaatagaat cccgagtggt attgagacat tgaccaacac ccctaagatg agcgagacgc
     1741 cgagtaatgc tagtccccac tcattaacaa agttaactat aggcaaaata tttggagtag
     1801 agagatattc aaacaactgg ttgaaggcct ttgctccact caagtatccc gaggcagacc
     1861 agtttggatc aagtattttt gtaattccag cccaaaagaa catccagccc atggtgacac
     1921 gaagcgccaa aatggaaact ttttgtatat ttgtcatgaa ataattatac caaagtttaa
     1981 cccaccagtg ccttaaccct atcctcaaga ggtggatgag ttagaaacat atttcttaca
     2041 aaagaaaaac tcttagattc cctcgcaaac ggattagcaa taaacatatg cgcggttgca
     2101 gtgttcgctg tcttcatcga gatcgaattt gtgtttatct tccttagagc actcgcaagt
     2161 ccttctggaa aacgagtcag gagcgcccca gacgcatcag cgaggaactc tctcttcctc
     2221 gatattgcca gttggataag agtagcggct attggagcta aaatggctaa aattaagcct
     2281 aaaacaaaca tcattccgcc cgcccggctg tctcgatctc tagaattatg catagtagac
     2341 ctaagaaata tatccgaaag tatggtgata aaaccagcca aaaccaccac cacagttgag
     2401 atcaaaatat ctctgttgcc tatatgcgaa agctcgtgtg ctagcacacc ctctaattca
     2461 tttttattca aaagatgtaa caagcccgtg gtcacacata ctactgcatg atctttatcc
     2521 ctacctgtag caaaagcatt cggggccgca tcttctataa tgtagagcct tggcataggt
     2581 agtccggctg tgatagcaag attctctaat gaattatata aatctctctg accatctcga
     2641 gttaccggaa tagctttatg gagcttaagg acaatcttat ccgaatacca gtaagaaatg
     2701 acattcataa gtaccgcaaa aataaccgca atgtaaagaa tattactgtc tccatatatg
     2761 caagagaaag tgtaaccaat cccaataaca actaccaaaa acagggccat aagcagccaa
     2821 gtcttacgga tatttttgtc cgcttgagcg tagatattac tggtcattta gtttagaact
     2881 taacttgtac agggttcttg gcgacagact cgtcttctgc taattcgaaa aactctttct
     2941 tagcaaagtt aaacatttta gcaattaaat tacctgggaa ggagtcaaca cttgtattta
     3001 ggtcacgaac attagtgttg tagaaacgtc tagatgcttg gatcttgttc tctgtatccg
     3061 aaagttcgcc ttgtagcgag agaaagttct gatttgcctt aagatctggg tatgcttctg
     3121 ctatagcgaa gagtgatttc aaagaacctg agagttccat ctcagcctta cctttgtcct
     3181 ccctatttcc tgcttgcata gctctacttc ttgcttctgt caccttctca aaggcggtgc
     3241 tttcgtgcaa agcgtagccc ttaacagtgt ttactaagtt aggtataaga tcgtatctgc
     3301 gcttgagctg aacctcgatg tctgaccaag cctcttctgc tctgtttacc aattttacga
     3361 aaccgttata agagaatacc ccccaaagca ccagtacgcc caatattatc catatgtaca
     3421 tcataatgaa tttattctaa cacactctaa taagacatat cgctccctcc aagatctgac
     3481 tttgactcct tctttggctc ctcggttata gctacctcag tagtcaaaag caccgctaca
     3541 gcactagacg cattctcgat agcacacctc tcaaccttag cagggtcaat tatcccttct
     3601 tcaaacatat tctccttaaa ttcgtcattt agtgcatcgt aaccgaagtt ttctccaccc
     3661 ttcatcactt ctgaaacaac tactccgccg tccatacctg cattaattgc gatctgcttc
     3721 aaaggcatcc ttaaagactc aattagtgct agataacctg cagagtactc actcgagagc
     3781 acactctcat gagcagtctt cgcctcctta gatagtctaa acttctcttc aagaaatttt
     3841 gacaccttaa ccaaggcaga cccacctccg atgacaatac cctcggagat agcagccttg
     3901 gtggcattga ccgcatcctc gatcttgtcc ttaagatact tcatctctgt ttccgttgct
     3961 gcaccaaccc ttattaccgc cacgccattc gtgagcttac cgatcctttc atccaacttt
     4021 tccctatcaa acttgttctt actattctct ctttgtgatt gtaggttctt aacgcgctct
     4081 aaaatatctg ccttcttacc cttacctccc acgataatag tgtcatcttt tgtggaaaca
     4141 attttctgcg cacgacccag gtgaccaata tctaacttct caaatgtaag ccccacttct
     4201 tctgaaacaa atttcgcccc aaccataata gctatgtctt gaagcatatc cttcttttta
     4261 tcgccgaagc ctggtgcctt aactgccaag atgttaaatc ctccacggag tttgtttaag
     4321 acgaatgtag taagagcctc cccgtctaca tcatcagcaa cgattaccaa ctctttcttg
     4381 cccgactgag caatcttttc taaaagtggc aggacatcct taatggagga aattttcttg
     4441 tctgtcacta gcactaatgg gtctttgtat tctgcctcca tgcgctcggt attggtgatc
     4501 atgtatggcg agatataacc cttatcaaac tcaaatccat caaccacctc actctcaata
     4561 ccaaatgatt gagaggcctc gacagttacc acaccatcct taccaacctt cttaatagta
     4621 tttgcaataa tcacacctat ctcctctgac tccgcagaaa tcgtcgcaac ttgcttaatc
     4681 tcctcaccag tcttaattgg cttagacatc ttcttcaaat gagccactgc gtcccgggtt
     4741 gcagcctcaa tacctcgtct tatacccata ggattcgccc ccatcatcgt ctttttgaag
     4801 cccgcctcaa ccaatgcctg agtaatcacc acagaggtag ttgtaccgtc tcctgccaaa
     4861 tcatttgtct tctgggcaac ctccttcact atctctgctc ccatattttg gaatttatta
     4921 ggcagagtaa tatctttagc aattgtcaca ccatcgttcg ttatcatcgg tgctccgtaa
     4981 ccacgatcta tcacaacatt cctacctcga gggccaatag ttatgcgtac tgcattggct
     5041 acctgatcaa ccccgctttt caactccttt cttgcttcat cgccccaaat aatttgttta
     5101 gccataaatt tacttaatta aagctagtat gttctcagat ttaaccaaat aaagatcttc
     5161 ctctccttgc ttcacttcgt cgtaactata tttgctaaaa atcaccatat ccccaacctt
     5221 aagcttgata ggtatcaact tcccatcctg gtatctcccc tctccaaccg caagcaccaa
     5281 acccctatct gacttctcgc ttgttacact ctctggtaaa acaattttta ttttcccgat
     5341 actacttggc aaatcattac ccattggtcg tataagcacc ctgtctccta caggcattat
     5401 ctttgttttt ttgattactt tcttcatagt gactggattt tatcatcata gatataattg
     5461 atgtcaaggt aagagctatg ctatgattat ttcctaaata tatgacaaac atacttccat
     5521 acatccaagc cgttctctct gttgtattag tagtagcaat ccttctgcaa cgctcagacg
     5581 cgagcttggg aagcgctttt ggcaacgaac aaggtaacac taaattcaca agacgaggtt
     5641 ttgagaaatt cttgtttaac gctacaatag ttattgccat cctttttgca gtttctgcta
     5701 ttttgtctct cttctaccgt tcttaacata gctctaatat ggagaataaa aatttccgag
     5761 cgcttttgag taagcgtttc cacatcccca aagcacgcac catagaaaga gctcttaccc
     5821 gctttacctc agcagagaaa gctcttttct attcactgac atttctcctg gtgctgacaa
     5881 gcattattat gctcctcaaa gtaaacagtg ccttcttagt aagagtgccc attagtggag
     5941 gctcagtaac agaaggagtt gtgggcaacc ctagatttgt gaacccagtt ttagccatat
     6001 ccgaagcaga caaaagcctg gtctctctta tctattcagg tctagtgaga ataggtgtta
     6061 acggagacac ccgtaacgac ttggcagaga gtattgtagt gagcgaggac ggcctaacct
     6121 acgaagtaac cctaagaaag gatgcagtct ttcatgatgg aagaccacta accgctgatg
     6181 atgtggtctt caccattcaa aaagtcttag acccaaatat caagagccct cagttcggtg
     6241 attttgctgg agtcactata agcaaggtgg atgatcacaa aataagcttc tcccttagga
     6301 aaccttattc tccattcatt aagaacttaa ctataggcat actaccaaaa cacatttggt
     6361 ccacagtaac agatgacgaa ttctccttca gtcagtggaa cgtcttaccc ataggctctg
     6421 gtccatatat ggtagataaa accgatcgtg actctggtgg aataccaaat tattacaacc
     6481 tgattccctt tgatgaatat ttgtacgaca aaccatttat ctctaattac atatttaaat
     6541 tctacagttc cgagacggac atgcttgagg catatgatgg gggtgcgtta gacattctag
     6601 gcggcatctc acctgacgaa gctctcacaa taaacaaaaa cagatccgac atcatcaact
     6661 ccccactacc ccgaatattt ggtgtcttct tcaaccagaa tgcgaataaa gttctactcg
     6721 acaaaactgt tagagaggct ctcgacatcg ccgctcctaa agaggaaatc ttgaagatag
     6781 tcttaaacgg ctttggaagg attatagacg gtccattacc accaatgttc tctaaagcta
     6841 gcacaacaga atcatctaaa actgccgcag aaagactaga actggcctca agcactctct
     6901 ctaaggctgg ctgggtaaag aacaaagaca caggtatact tgaaaagaaa aataagagtg
     6961 atactatcaa gctctcattc tcgatctcga cgagtgacaa ccccgatcta gagaaggtcg
     7021 cccaaatgct agaagctgca tggaccgctc tcggtgctga ggttaaagtc tctatctacg
     7081 aatctggtga tctcaatcaa aacgtcatca gacctaggaa gtacgaaact cttctctttg
     7141 gagaagtaat cggtaaggac ggagacgctt tccccttctg gcactcaagt gaaaggaatg
     7201 atccagggct aaacattgcc ctatatgtaa acaaccaagt agacaaaatt cttgaaagca
     7261 ttagatcaga gcgagatgaa gataagaaga acgatcttta cctctccttt gagaaagcaa
     7321 tcaagggcga catcccagcc gtattcttgt attcaccgga ttatatctat gtgataccag
     7381 aaaaacttaa gggtattagc ttggaaaaat tgtctaagcc tgaggataga tatagaggta
     7441 cgacgggttg gtatgtcgag acaaattcag tttggaaggt attccagaaa taaatacaga
     7501 ataaataaac aattaaaaac ttaaaatgaa taatcagtcc aatttcaaaa gaggtctaag
     7561 aggcaagaac atgcacagga ggaacgaccg cccaatcgcc gagaaaagag ctccagacat
     7621 acttccacca cttgctcctg ggaacattcg tatcgtacca ctaggaggtg tagaggaaat
     7681 cggacgcaac atgatggcta tcgagtacgg taacgatatc attgttgtcg actgtggtat
     7741 ccagtttacc gatgcagaga ctcctggggt tgattatatt ttgccaaatg ttaaatacct
     7801 cgaggaaaga aagggtaaca tccgcgccat ggttatcacc cacggacacc tagaccatat
     7861 cggcggtatc ccctacatca tgaaccgtat tggcaaccct cctatataca caagagaatt
     7921 cggcgcacta ctcataaaga agcgtcaaga ggagttccct catatgccac cactagactt
     7981 gcgtattgta gatcagagcg acaagtctat tgctattaca ccaaacttaa aggttcgctt
     8041 cttcggactc acgcactcta tccccgactc aaccggagtg attatcgaga ctcctctagg
     8101 cgacatcgtg aacactggag atgtcagagt tgataacgag aatggtgtgc caacagataa
     8161 agaggttgaa cagtacaaga tgtttaagga tagaaatgtc ctcctcttca ccatggactc
     8221 aacaggtatc gagaagcccg gtttctcccc ttctgaagac ttcattctaa aaaatatcga
     8281 ctcaattgta gccaatgtcc ctggtcgcgt gatcatagcc actttcgctt cgcaagttga
     8341 gcgtatcatc gagttcatgc gcattgccaa gagatatggc aagaaagtag taatcgaagg
     8401 ccgaagcatg aggaataatg tggacatcat caaacacctc aatctagttg aacttgacca
     8461 cattgttccc atcgaagaca tcgagaagta tccgccaaac aaaatcatga tgattgcaac
     8521 tggtgcccaa ggagaggagt tcgccgcact tatgcgcatg tcaaacaaga ctcataaatt
     8581 catcaagctt cagagatcag acacggtgat cctatcctcc tcagtgatcc ctggtaacga
     8641 acgtgcaatc gcaaaactca aggacaatct atttagacat gatgcgaaga tcatcactta
     8701 ccttgattcc gatgtgcaca cctcaggtca cggtaagcgt ggtgagctcg agtgggtcca
     8761 taaacagata ccttacaaat tcttcatgcc tgtacatgga caccactaca tgctcaaaat
     8821 gcacgcggag cttgctcgtc aactcggtgc agcaccagag aatgtcattg tcgcagacaa
     8881 cggctctcta atagagatta gagaggaagg caaaattatt accagagcag tggagaaggc
     8941 tccatcagaa gacatggtgg ttgaagggtt ctctgtggga gatgtctcag aagtgctcat
     9001 cagagaccgt aagatgctcg ccgaagaggg cttcgttgtc gtagttgcta tggtagtaac
     9061 aaaaaccggc aagctaagga agtcgccaga catcatctct cgcggattca tctatctacg
     9121 agacaaccag gagctactca ccgagactcg ccacatcatc aagagagcaa ttgagagcac
     9181 cactgtcggt atgaacccaa ttaacttcga ttacgccaag aacgctgtgt ccgaagatgt
     9241 tgctcgcttc ctcttccaaa agacaatgaa gaagcccgta atcatccccg taatcctnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnata tttgtggcga acttcttttt
     9421 ttctattcat tttgccgcca ctctttatat caattcctca ttcctcggac acttcttctc
     9481 cccttccaca atcggttggt tatatctact tggcgcgctt ggtaatgttt atttcttaat
     9541 gaatgccgag aggatgctaa gctacctcgg gagcaagggt ttcctactct tcttctctgt
     9601 cacaactctt atctcaacca taggagcggc tctagccacg acaccgggca tggctaccat
     9661 cgccttcctt atctatgctt ctgtgtcgat gatgatctac tactccttcg acatcttgct
     9721 cgaagaagtc tctaagaatg aaaatactgg taagactcgg ggtatatacc tcacaatcac
     9781 caatgcagct attatgggcg gacccgtcct catcgccttc gcaggagatc aaaacaactt
     9841 caaggatcta tacatactct ctgcatactt cctcatacca atattcctcc ttatgatctt
     9901 ctcacttaaa acgaagcgag gtgtagaaac ttatctcaag gttccgctcc cctttagcgt
     9961 atggaaggag ttaaagaatg taagacgagt aacacttgct cgcagcgtgc ttgagttctt
    10021 cttcgctgtc atggttatct atatgccgat ctatctgcac aacattgtcg gcttctcatg
    10081 ggaacagata ggtatcatct tcaccttcat gctcttgccc tttgtcctct tcgagtggcc
    10141 agcgggagaa cttgcagaca agaaatatgg cgagaaggaa ataatgtttt caggattcgt
    10201 catcttgtgt ctctcagtac tcatcatgcc gtatctaggt agcagtatga tcatctgggc
    10261 catagcactc ttcgtctcgc gcattggggc tagtcttata gaaatcacga cagacagtta
    10321 cttcttcaag caagttggtc cgcatcatgt ggggcttatc tcaatcttca gactctctag
    10381 accggttgga ttaatgctcg gagcaggtat tggtgcccta tctaacgcgt tcctacacca
    10441 gagctcaatc ttccttatac ttggagtgat gatactctat ggcattagac agagcttcct
    10501 tctggtagat accaaataag aggtttattt atactcgaca tgatctgtga aggttctctc
    10561 aatctcatct gccttcctta actttaattc cttaggcact atatcggtat gtggagagtt
    10621 atccaatgca ttaactaatt gagagagatc atcgtcacca agcaaccagt cgaatttact
    10681 tttcgacaga tcaaatgggg cagaatactt tcttaaagtc ttggtaccat cggccaggta
    10741 atactcgtgg aagtaactca tcgcaagctc tcttggagtc ttatacacgg catcgcggaa
    10801 gcgaagtacc gggtgattcg tcttcgaaat agctccccaa agtgctccta ctttatacaa
    10861 agcgactaca tgatcctggt ccccttttgc agtccttaag tccataagta agggtttatc
    10921 tccttgtagc caaagtgcgg cacaagccac aagtgctcct tcgaagcagt gggccttacc
    10981 gctttctagt gtcttcaata ctgagtgata cgtctccccc ttcctctcgt ggttgaaggg
    11041 tatattattt aaaaaatttt gtattttatc cggagtgttt agtttaccga gcactctaag
    11101 tgctttgggt acgtccttca tcttatttga tgaaggttag cgagctcgca atgtcttgaa
    11161 tgttagctct gtcggcccaa aagaccaaat atttatatgt cgtagatcca acaaccttag
    11221 gtgttggata ccttactata agcacagcct gtccgtgcat tgcggggctt tgatcgagtg
    11281 agggtaagat gagtcttgcg tcctgcccat ctatggtgag tgtctgtatg tatggctcgc
    11341 taccataagg cttcttgtta ttttttgcct cactctgagt cagtgtgtct atagaaacat
    11401 tatcctcacc cccagcagaa atgctgaaga atccactctc tccttcgtac ctatcaaacg
    11461 agagtccacc gacgggttgc cactctggtg gatagcgtaa gttgactcca tagacatagt
    11521 tcgtgtagcg cgctaccaca ggcactggct ctcctcgcct tacgttggtt atatgctgag
    11581 catagataat tatgagtaca aaaaagacta taatgccaat aagtaccatc catatcttag
    11641 tatcggaagt atcatttacc ccttgaggat gcataatcgt tgattataca tgattagtgt
    11701 acctccgtca caagctcggg ttgagcccct acatagtcga cactaaacca tatcctaccc
    11761 aagacctgac ctctctcggt cataacattt actctccagc tgcctggcac tacactcgag
    11821 attgagtatg ttcggtaacc attatttcta cccccagtaa tcggcaagag aactcttgag
    11881 acacttaccc actcatcttt accctgatca aagtactgcc actcgtgtac tatcttagtg
    11941 ctaaactttg ctggactaaa tattgcggta taaacgaaaa gtggttgatc aggtaggata
    12001 ttcacgctcg tccttggact aagcttctcg agccaactct tcctctcagc tcttagtaca
    12061 tattggccgg taatctcttt atctacaagg tgatacacac ctccctcacg gagggaaagt
    12121 ggtataggtg ggatgatgtt tgtgaagtaa agtatgttaa ccaatgcaaa aatggcgagc
    12181 actatccccc gtatggatcc gagggctagg gtaaacttct ctccacttac tctttgcaga
    12241 attgctagaa agaaacgcag aacaagcaaa ctgatcaacc cactaaagac gaaaatagtt
    12301 ggtccgatgt accctaccac caccggcaca aagaagatcg agaaaagtag tagagcagag
    12361 aagaagaagg acacttgata agtcagtctt tgataattcc tcttcaacct ttcgttggcc
    12421 acgaaggcaa gagcgagcat gaggagaaag ggccaactca ctgctagtga cgaacttcta
    12481 aagtagagga tgagaaatgc agagagcaat ccaccgaaac taaattgcat catactaaga
    12541 agccagaagt cggtatcgga gtcagcatct ctgtccttac tcctccgatt tattataagt
    12601 atggctgtgg caaccacgat aagatggaac acaacccaaa agttctcaaa aaaggcgtcc
    12661 gctcttctaa gtgtcaaaac atcgaagatg aaccccaaaa caagagcaat ggacgaaatg
    12721 tgatactcgt aattcttgta gattgagttt agcttgctcc agagcatgta attgtatgcc
    12781 aaatataccc cctgacccgc ctccgagcaa gtaggacaaa caaccctaag cttcagtcac
    12841 cacgccgtca aagtgttcct cattggcttc ggcatctgcc ttggtctcat gtaccgtgcc
    12901 gtccttgtgg ttcggaggag agtagacact atagagtttt agaggcttat cgactgaagt
    12961 gtttattaga ttatgcttag ttcctgcggg tacgactata gcagagccgt cacgaacctc
    13021 gtgccctacc ccatcaagca ccacccttcc aataccttct tccaccctaa gaaactgatc
    13081 caactgatgc acctcttctc caatgtcctg tagtggctca agactcatca gcaccaactg
    13141 agagctcttg gctgtgtaca atactttcct aaaattatta ttctccaacg tctccttctc
    13201 aatatttgta atgtatcctt tcatgatatt aattatatta aagttatctg aacttcacat
    13261 gagttatctg atagaggcta aagacaacgc ccgagttaac tactaaggcg ccaaatatta
    13321 aggcccaatc atcataataa atccccttaa caaagaggat gcattgaacg ataaaaacgg
    13381 ctccgggtag gacaacagat acatgctcgg cactcttact cttgtgaacc acatatcctt
    13441 ggtgataata accaagcgag acaataatcc agccacacgc tagcacaata ctccacacat
    13501 tctgagcact ccaaaagtcg ttcacaccaa ctaattgtcc atatacaaag aatgtggcaa
    13561 cggcaacaaa tgctaaacta aaaaatattt ctataaaaac cttcttcata agccaatttt
    13621 ccttgctata aagtatgcta tgacacttgt cgctcctgag agcaatgcac cccaaagcat
    13681 atcaacaaca gtcatcatga gtggccagtc tcttatggtg gcgtggttcg taagatcgta
    13741 cgctgcataa gatatgaagc ccaagaagag cccgagtaat agggccttgg tccaaacacc
    13801 ttctgatagt gctggagaga taacaaagat aacaagagct agagcaaata acaggtaaaa
    13861 gataagtgcc ggtacaagat taacctctgg cttcataaga tagccgattt tgtcttggta
    13921 gaagcccttg gccacaaacc tgagccacac taaatctatt gccaaaagca ctggcagggt
    13981 gatgatataa agtttaatta gcatcgttta attatacccc accgccaagc cctcnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcttcc cccgctttta ttcttcttaa
    14161 cgaatagaca aagactcttc cgcttggacc tctggcctca caattaggtt tgcacgaatg
    14221 attgataaat ctcgccacat tctccttaat gtttccgttg atcattttat tcctcccggt
    14281 cgaaaacaag tacctccctg ttgcaaattc ttgctcctct attgggatat ttctaccaat
    14341 gtactcaata attcttttcc ctttaggaat tgtttctaga gcaaaaaccc ccttaccaga
    14401 aagaccgcgt cccgtacgca gtttaaattt tgtgaccggt gtttcaagtt ttaatgacat
    14461 atctaaagcg tgtatattcg aaacaaataa atcaattcac tagctgggat acagggattc
    14521 gaaccccaat taaccgctcc agaggcggtt gtcctaccat tagacgatat cccaataagc
    14581 ctagaatact tgaattttgg ccttatttca agatttgatt tttagtctag ttgctgtaat
    14641 ctcttgtagg agcagtacct ttttacccca ttccggagaa cgccaatgct cgtggtccca
    14701 ctcccgatca gaagtctcat cgaagaacac cgtcgcaacg gcacagctcg tccgggccga
    14761 gctcccatga tgaccacctc acagaaaggt gacgtgaaga gatacggcga ggacctcaaa
    14821 gctgagatcg cgagactcga tatggagctc agaggcctga agcaacagca gctcgatctc
    14881 tcttcgatct gtgatcatga gaggcaaacg aatggcaagt gcaagcattg cgaccagccg
    14941 gtcctcgcta tcatggcgta ggccaacaca gctgtcccga aaggacagct ttttctttat
    15001 ataacagtaa gagaacccac cataccctgt tgtctgtgtg taccaactga gcagtaatac
    15061 tcgaatgtac cagacttgtc tgcaacgaag gttatcacct cattctctcc tgccttgatc
    15121 tgcttggtct tagcaccgac aaactcatct attacaaagt catggaagcc gactttgttt
    15181 atgaaagtga tcttcaccgt atcacccttg ttcaccgtca tggtctttgg agcgaaggcg
    15241 aaactactcc cctcaacagt aaactcctta acactaactt tgttctcagt ggtgttccct
    15301 actggagaaa cagcgttgcc cacactgtcg agtagtgtcc caccttgtcc cgtcttcata
    15361 agcaagaagc ctccaaccaa tacaactgcg actccaagta atgcaattat tttattcatc
    15421 aaattaaatt actgcagata atcactcgaa tactttatcc tatactctca agtacctcct
    15481 gatagcaaga agtgaggaca cgactcccac cgctacaccc gcaccaagaa gagtgaagaa
    15541 aatagaaaca aagtgaccga tatagtaact aaagaggttt agcccaatga agaagtcttg
    15601 cgtcacacca ccgatccaat atgtaaccgg aaggaagata agcgctgtga gtatagtggc
    15661 aacaaccgca tagataacgc ccagggtgac aaatggaccc tctatatagt tcgtcgaggc
    15721 accaaccaat cgcataacat tgatctcctc acgagagata taaatggtaa ggcggattgt
    15781 gttgaaagcg ataagtaccg aaagaaggat aaacgatagt gctatagcaa ccccgagttt
    15841 gtcggcactc ctaacaatgc cagttagttt gtcgatggcg accttgttct gataataatt
    15901 tacatgatct actatcatta gtccgccact agatagagca ctgttacctt cgagaaactc
    15961 agcgatactc ttgtactgcg agggctcttt ggccttgatg tcgagtgctg ctcctagagg
    16021 attctcattc agctcgttta atgcggtgag tatagactga tcattctcat ggcgaagttt
    16081 aaatgcttca agtgcctcct ctctggtggt ataagtgact agcgagacct cagagagtgt
    16141 ctcgagtgag ctcttgatct tcaatatatc ttcctcggga gcagaaggga tgaaggtaac
    16201 agatatgtct accttatctt taagagtagt aagtgcagta gtaagcaccg ccccaccaag
    16261 catggtgaag ccaatgacac taagtgtcgc cgtcatcata aaaacggagg ccagggaaac
    16321 ggtaccattc ctccagaagt tcataaaccc ggtcttagat attcttttaa agttagtcca
    16381 gagcatgttg gtttctattc tactatataa agtacttacc ctccttgtcg tctcttacaa
    16441 tcttgccctt ctccatagca accacgcgct tgcccaacat gtcgatgata cccttattat
    16501 gtgtggtgag gatgactgta gtgccgaagt cgtttatctt ctttaagatc tgcacaatat
    16561 cgtaggtatt caccgggtct aggttgccag taggctcgtc ggctataagg acatcaggct
    16621 ggttaaccag ggcacgagct atggctaccc tttgcttctc tccgcctgag agctcgtgtg
    16681 gaaaattcca aaccttattt gccagatcca ccagctctag aacatggggt acgtcttgct
    16741 caatctcctc atcagtcttg cctgaggcct ccatggtgaa agcaatgttt tcatatacag
    16801 tcttatttga aataagacgg aaatcttgga aaatcatccc gatctttctc ctcaagttgt
    16861 tcatctcgtt tttactcaaa cgatgtacat ctgatgactc gtagaaaacc ttaccctcac
    16921 ttggtttctc ctcggctaag agcatcttca aaagcgtggt cttacccgca ccagattgtc
    16981 ccacgataga tacaaattcg tttggctcta tcgtaagggt tatgttttga agggctaccg
    17041 aagtatccgg gtaaactttc gtcactcggt caaagaaaat catcctcata ttttaacaca
    17101 catgctcagg gtataaaaaa ccactctaac aagagtggtt agattaccaa acctcatcct
    17161 caggaagcgg cccatcactg atagtatcgg cctcaatact cccaatgatc cacctctgat
    17221 gattcggctt gtcgcaactg tagtagatct tgcccctatc aaacccagtg atgccagaac
    17281 caaaatgact taaaccctcg attgtataag tagccttatc accacaaacc gcacaatacc
    17341 tagcttgctt ctcacggaaa acctctttag cgagaagcat gtggtaggtc ggctccacaa
    17401 tctgacgagg gcgaatcttg tgtccattaa ctgacatgta aggtttatct cgactgccaa
    17461 gcaccaccac cactccatca tctttgtcga agctgtgatg aatcagagat cgatggaaca
    17521 ccacagcccc attgcatgga gcccagtgct ttgcttcgaa gtaatacttg tcagtctcct
    17581 tgtcctgcca gagatatgca tagaggaccc gatacctttt catcctcctt ctctcaaaaa
    17641 acttccacaa gagtctccac atacaataat ctccaatgct gaggaagaac ttcgtctcga
    17701 gggattctac actatttata aacctttttc agcttctata aactcgtcga ggtcgccatt
    17761 caacacccgg tctacatctc tcacctcaac atctgttcta tgatccttaa ccatcttgta
    17821 tgggtgtaac acatatgagc ggatttgact cccccactcg atcgaagttg ttttggaaac
    17881 atacatcccc ttctccttcg cctttctctc ctcgtcttgt agtttatata gtttggcctc
    17941 aagcatggct agagctctct ccttgttctg tgcctgactc ctctcgctct ccacatgcac
    18001 cgcaaggtta gtgggtatgt ggaccacgcg cactgcagtc tcgcgcttat taacattctg
    18061 tccacccgga ccactagact tcgaatactc aagccgaata tcttcaggcg gtatgctaac
    18121 ctcaccaacc ttctcgaact tcggtattac ctcaaccata gagaaggaag tgtgacgaag
    18181 cttcttggca ttaaagggcg agatgcgtac caatcggtgc acccctgatt cgttcttcag
    18241 agtgccgtac acccctttac cgcttatttc aagagtgatg tttctatatc ctccatggtc
    18301 attatcattc ttgtgcatta cagagtagct ccacccctta ttaccaaaat acttcaaata
    18361 catttgaagc aagatgtttg tgaagtcctc tgcatcgtca ccacccgcac cgctaaatat
    18421 agtcatcacc gcatcgccct tgtcgtactt gccgacaccc tctaactcgt ccttaagatt
    18481 ctgaagttcc ttaatagcac cttgtgctga gtccttatca ttccaaaagt tagcgtcttg
    18541 catccgctcc tctagggcat caatcttctt ctgtatttca tccttactgg ccatgtattn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gagccaaagg tgaggatcga
    18721 actcacgacc tgcagtttac gatactgccg ctctaccaac tgagctactt tggcacaaga
    18781 agcattctaa cacttggagc caagacccgg gatcgaaccg gggacctctt ccttaccatg
    18841 gaagtgctct accgactgag ctatcttggc acaaaacacg aaagaacagg cccaatagta
    18901 ttacaaatca gcctcttttt aaagtgcagg tggattattc aaacacatcc ttaattcact
    18961 agtgtctgtc catccacact gaccacttgc ctgtctttcg cacttagcac ttctatagca
    19021 tgcgtactct gccctaaact cacacgtaga aaccatttct tctttatcag agcaaatttg
    19081 gctcgagcac ccagcaacaa agcaaccttt tgcccctatt ggagcctgag ctcttttggt
    19141 tacttcgaag acaaatttgt aatcattagc tagagtctct ccctgcatct tctcaggcga
    19201 aacagaaacc aactttattt cctctgcttc agtggtgatt gaaccaccta attctattgt
    19261 gggagtagaa gcaccaagac cagaatatac ctcaacttta accttaagag tcccagccca
    19321 tatgcaatta acattcaccg gacaacgact atcctgaaca acctcggtcg gcttgataga
    19381 gatatttcta aatacacctg actcaccaat cttaagagtt atctgaccct cttccggttc
    19441 cttagttgga aagacatctc caataactac cgaatccgaa gaattaattg gtgcataata
    19501 atcaacaaac caagccacac ctgccacgat cgtaaacact aatactaaaa gtattagaga
    19561 tgttaactgc ttccacatat aaaagaaact ttactctttt ttgatcaagt agtacagagc
    19621 gagtggtgct ccccagttag caaatctttc gataaagtcc cagattggca taccaactat
    19681 tggcctcact agtgctgtcc agaatcccca gaacacagcc caaaggagta ttggcttcat
    19741 cggcttaact aagacaacta gtgccacaat gagatccata ataccaacaa gcatgagtag
    19801 agtggtcgcc gtagccgggt cacttactcc aaacttagca aaccacttaa cccagtctgc
    19861 cttgccttgt agagcaaata ctgcgtggcc caaaaactcg cctgctacgg caatacgcaa
    19921 gacccaaaca gccttttgtg aattggacat aaatataaaa attactaata aatcccttat
    19981 aaattatact cctaatttag gaaacaaaat tgttgcgggt gcccgatttg cacggacgcc
    20041 tacaggttat gagcctgtcg aggtactgct cctccaaccc gctgtagaaa gccattatat
    20101 cgcactctac tgacaaaatc aatgtaaaat agtatagtac tagccacgca atgtgagaca
    20161 gcttacggca gtaagccaaa gcttgtcggc gtagctcagt tggttagagc gtgggactca
    20221 taagcccaag gtcggaggtt cgatccctcc cgccgacaca ttgcggtcat agctcaactg
    20281 gttagagcac tcgcctgtca cgcgagaggt tgccggttca agtccggttg accgcgca
//
LOCUS       DAORIT010000008        18848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_474_length_18848_cov_51.625020, whole genome shotgun sequence.
ACCESSION   DAORIT010000008 DAORIT010000000
VERSION     DAORIT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18848
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_474_length_18848_cov_51.625020"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1284
                     /locus_tag="PLD99_01205"
     CDS             151..1284
                     /locus_tag="PLD99_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25554.1"
                     /translation="MLTALLVLLILTAWFLAAGLWFVKSGTIVAVLRFGRFDRVARPG
                     PNIIIPIFESGERFSTQTHQTEYPLPDSSGKKTPFRILQKGLREAKFYKLKDPNLDPS
                     DPESYEFVYVDEFIKENGVQNTTLLAAMENDPLHSPLTSEVAVVAEWNLYAEMIRVQR
                     FIENIEPRDGRTREEEVDKRIEDVIAKTLQEFLGEITVGHARDRFPLLNGLIKLRLEE
                     LVGEHLTNGVPDEKAWGINVTKGYIKNIDPGHTVNKQRSEAAGSVSEMQKTINAAEAE
                     AVATVKAGNAEAKKIEAVGKATKEATIMAGDAEAHRIRVTGDATKDAMMKQNDARADE
                     IERVVKPSAADPLMVEVVKAHAYRDNGTVIAVGGTPLINTSPK"
     gene            1407..2069
                     /locus_tag="PLD99_01210"
     CDS             1407..2069
                     /locus_tag="PLD99_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25555.1"
                     /translation="MTLTQWFILTIGVLGLIAIVVYKSGWRPKKAAVAPTGATVVPGK
                     KSEGWWKKNFGSVIMIAVFGYVAWAMYAHGDRVVKSTDSTRALANALAEANQMKKDAS
                     KLFVDTLVIFATEEGSPTIEIPKGAKVRFERMEDVSYALHYETENGRTGTVEFRKELE
                     SIISLPPDLKKVEFLSLEKSSVRIIFMSTKPLEHRVTPYEGPRIIAGAVFYLHLAVRY
                     GI"
     gene            complement(2040..2324)
                     /locus_tag="PLD99_01215"
     CDS             complement(2040..2324)
                     /locus_tag="PLD99_01215"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25556.1"
                     /translation="MDISRFLNSVNAVIINPIIVLVFAIAFLVFFWGLYQFISKAGES
                     KDREEGKKKILYGLIGMFVMFSAYGLMRIVLNTFGISSPQYLNTVPNRQM"
     gene            complement(2335..2679)
                     /locus_tag="PLD99_01220"
     CDS             complement(2335..2679)
                     /locus_tag="PLD99_01220"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRH25557.1"
                     /translation="MKKLLYTALALGPSVAFAQTLGGVNTLLDSISRLVDTALPVVVG
                     LALLAFFWGLMKFIFAAGNEDNKEDGKRIMIYGLIGLFVMVAVWGLVGFIADNLNVDT
                     GGSANVPTVPGL"
     gene            complement(2782..3060)
                     /locus_tag="PLD99_01225"
     CDS             complement(2782..3060)
                     /locus_tag="PLD99_01225"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25558.1"
                     /translation="MQNLRTLIIEFGNLVELAIPIVAGLALLGFFWGLAKYIFSQGNE
                     QSKIEGKKVMVYGLVALFVMFSVWGIIGFFQRDLNIDPGAGIIQIQTI"
     gene            complement(3073..3360)
                     /locus_tag="PLD99_01230"
     CDS             complement(3073..3360)
                     /locus_tag="PLD99_01230"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25559.1"
                     /translation="MPTDLRSLVNIFLGLINPLILVLAGLSLLMFFRGLLNFIIKSDD
                     TKSLEEGKEMMKWGLVGLGVMVSIWGILTFFYSGFGWGQMGFPLLPENGRV"
     gene            complement(3360..3752)
                     /locus_tag="PLD99_01235"
     CDS             complement(3360..3752)
                     /locus_tag="PLD99_01235"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25560.1"
                     /translation="MKKFSPHIFAFILVFFISVTFVSAQRGGETVSGGSGSIPNPFNC
                     GGAGGNCNLFMFVNTIVDRVLLPIGGILVVLAFIYSGFKFVMAQGNDGELKTAKAALT
                     YTAIGAAILLGAKVLTTVIENTVNSLRA"
     gene            complement(3755..4081)
                     /locus_tag="PLD99_01240"
     CDS             complement(3755..4081)
                     /locus_tag="PLD99_01240"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25561.1"
                     /translation="MDRIWKPLIVCDGALGTSDPCGFDDLIELANVVINNLTVIAILG
                     ASIAFIWVGFQLLTSQGDAGAMKKAKDAGVNVLKGLFFVLAAWVIVKTITSVLLSPEF
                     LNSSII"
     gene            complement(4085..5368)
                     /locus_tag="PLD99_01245"
     CDS             complement(4085..5368)
                     /locus_tag="PLD99_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRH25562.1"
                     /translation="MSKFQIVLLVVFGAFIVIAVILFSQNRGSGTSKTNITIWGSLSA
                     YDFSDLVSTAGLSKDGTFSYNYVEKAESTLSKDFTDALAVGKGPDLIILGVDSIMSES
                     EKLMLIPNESVRPADFANTFISEAKLFQTTLGTYALPLYVDPMVMYWNREMFARASLA
                     VPPVYWDQIYTYITKLTNKDGAGNITQSAMALGEAKNIPHVKEIMTLLLLQAGTPITA
                     FAGENLRSTLLESNGGAQIPSLSALEFYTQFANPQKSYYSWNRSQVSAENHFTSGKSA
                     MYLGFASELPTLKAKNPTMDIGVSPVPQSRASGKSITSGKLYGIALVRSTRDPAGALA
                     GALALVSKESASALTSITPVVPARRDLLAQPPTDPAGFTFYGAALQSDWWRDPEPKAT
                     RKIFVDMVESVTSGRARVDEAVSAANTALNNLLEK"
     gene            complement(5375..5917)
                     /locus_tag="PLD99_01250"
     CDS             complement(5375..5917)
                     /locus_tag="PLD99_01250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRH25563.1"
                     /translation="MFTDPKKNVLEFGFIPGQRVVDLGSGAGHYSLALSHALGEAGKV
                     VAIDIDKPLLSKLKNESMELGQTNVDVLDGNIEKLGGVRLRDAYADGVVFSNILFQLN
                     SIPTALEEAKRVLKPGGKACVVEWTDLTMLTGKKDYLQGKAIPEVDTKKFFEKAGFVF
                     ERSFNAGEKHYGLIFKKPLQ"
     gene            6046..6309
                     /locus_tag="PLD99_01255"
     CDS             6046..6309
                     /locus_tag="PLD99_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25564.1"
                     /translation="MTELRDSAKTKKSGTILLPSTVKQIPTVVKKGDHLFYVEGNGKL
                     RFAEDLDPMPISELPLSQIPPRLLTLVAGKQLAGEKIILYARN"
     gene            6449..6907
                     /locus_tag="PLD99_01260"
     CDS             6449..6907
                     /locus_tag="PLD99_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25565.1"
                     /translation="MKLKLLPLALIAVSLAMPPTADAEITKFNNGFIDQSRAAPADYT
                     LEVYARQLDFPSFILTSNPAMPDLIMVGLSEHRAYNASRLAATMVVVDQEVSPSGDLA
                     VMITSSTRLLTRSKNQTTAIDARSTTRYPATATNTDKAMTLVDLGIVNRC"
     gene            7050..7583
                     /locus_tag="PLD99_01265"
     CDS             7050..7583
                     /locus_tag="PLD99_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25566.1"
                     /translation="MVEFLDQLCTPHQKEKSVKIVDYLRIVGFCFALIAAPLVQFLSV
                     AVVLGMVAVSAEAQQTKIQVVVPVIEITRTNTPASTSYNSFIIDHNPSAARPNFEVSS
                     GEKVDIFFKASRATACQVDNPADNEPATGITLESNFSIGPQHGWYPAMGKTSTIKFSC
                     IDRFMKSTEAVVTISRR"
     gene            complement(7646..10084)
                     /gene="gyrA"
                     /locus_tag="PLD99_01270"
     CDS             complement(7646..10084)
                     /gene="gyrA"
                     /locus_tag="PLD99_01270"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRH25567.1"
                     /translation="MTDNTEDSKRVVSRSIVTEMKDSFIDYAMSVITDRALPDVRDGL
                     KPVHRRILYAMHQIGLTASAKTRKSATVVGEVLGSYHPHGDASVYDAMVKLAQEFSTR
                     YPLVIGQGNFGSIDGDGAAAYRYTEAKMSKLAQELLGDIEKDTVNFRPNYDGTKKEPV
                     VLPSAAPNLLLNGTLGIAVGMATNIPPHNLNEVVDATVMLLNDPDATTEDLLEFVKGP
                     DFPTGGIIYNQKDIAHAYATGRGGILARGVAEIVDDGKIEQIIITEIPYRVNKSTLIE
                     KIAELHREKKIEGIKALRDESTKDIRIVIDLKQGSQPQKVLNALYKHTQLEDTFHVNL
                     VALVDGVPQTMSLKGILESFVKHRDEVIRRRSAFDLAQAEAREHILLGLKKALDHIDE
                     IVKLIRASKTVDDARAGLMKTFKFSELQANAILEMRLQKLAGLERKKIEDELKEVQTL
                     IAYLKDLLSSDKKIRNLIKEELLKVSEKYGDERRTKVVKGGVKNISMEDLIPDEEQAI
                     VLTSGGYIKRTDPHEYKRQKRGGVGVVDLDTKEEDFVTNFLVASTHSDLLFFTDKGKA
                     YQIKMYDIPEAKRATRGKSIMNFINLSAEEKVTSILAMPKDAKNIEGSLVLITKDGTG
                     KRVSAKSFTDVRSSGIIAIRLDTGDKLVAVGHAEKGDTVSLVTKEGMSIRFKDSDIRE
                     MGRTAGGVRAISLDKKDEVIGAHVIKSAWKNAHLLVISANGYGKRTELDEYKIQGRGG
                     SGILTSKVTDKTGDVMASQVVTEEESEVIAISKKSQVVRVDIAEVPVLGRSTQGVRIM
                     KLRDGDSLASLICL"
     gene            complement(10074..10331)
                     /locus_tag="PLD99_01275"
     CDS             complement(10074..10331)
                     /locus_tag="PLD99_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25568.1"
                     /translation="MRKYLSELHTKPRHHKRRFALLTSAVVTLSIFAIWSGVKFGAKP
                     MVVKKETGTVNLAATASQNSLEKVVGSLKNSWKNLVTSDDR"
     gene            complement(10348..10914)
                     /locus_tag="PLD99_01280"
     CDS             complement(10348..10914)
                     /locus_tag="PLD99_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRH25569.1"
                     /translation="MIVTYQGQSSFKISQGSLSLSINPTSKTAADITLFTSKRSETSE
                     KSGFVIFGPGEYEVKDVSIKGFLSKGKGDLINTVYKMNVEGMNICFLGDLANSDLDSS
                     IIEELEDIDLLFVPVGGDGVLDPNTGYKLSVKLEPALIIPMHYSAESLKKFIKEAGED
                     KVDPIDKLVLKKKDLEGKEGEIVVLKEE"
     gene            11036..11530
                     /locus_tag="PLD99_01285"
     CDS             11036..11530
                     /locus_tag="PLD99_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25570.1"
                     /translation="MTKPADPHGQKVVEKDEDDVSWYTDWRWLGIILLLILMLGLVIF
                     RPWDKNKEEVAEAVSTPETNQVDSTSVTHSSPMITLPPAPKYCDVSDSTQTFCSSDPQ
                     TIEVLQNGELRLANLGEVRICARKPIKSITYKVVPNGKFDFTSSLDIENPRCDKFFVT
                     RGDQ"
     assembly_gap    11709..11808
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12611..13720
                     /locus_tag="PLD99_01290"
     CDS             12611..13720
                     /locus_tag="PLD99_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRH25571.1"
                     /translation="MSKEKVKKEKVVEVDNSLMGQLVAKSPNPPKVEDVVEGSVISID
                     KGAVYIDLPPYGTGIIYGREFMNARDIIKKINVGDAVAGKVVSMGGKDGYIEISLKEA
                     RQALIWSDADEAIRDKRVFDILVTDANKGGLILNWQGIQGFLPASQLKPEHYPRVNDG
                     DKDKILDELRKLVGEKLTVTIIGAAPKEGKLIFSEKSPETKSKESILARYTLGDELMG
                     EITGLVDFGVFVKLEEGLEGLVHISEIDWGLVENPKAMFKIGEKVKVKIIEIKDEKIS
                     LSIKALKENPWKDASKNYKKDDVVEGVIIKYNKHGALASIEEGVAGLVHVSEFGSEDN
                     LRATLEMGKSYSFKITLFDPKEQKMALSYIDKSKK"
     gene            13786..14310
                     /locus_tag="PLD99_01295"
     CDS             13786..14310
                     /locus_tag="PLD99_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25572.1"
                     /translation="MNVTVVVDTELNKRPKMGDRMIEEDNIKELIVFSVLVCLIRDKG
                     YRRDSVSVLKLHECWHLIKETKIEGVNVNWFRKNPVTRIWEELDRAIQRLVFSHWIER
                     GKIYRSSTLSLLTGTTTERKVFTEYWRIYSAILQIFPDFARLYDAIEEPVVIDGIREM
                     PIHGLENQVSTPEA"
     gene            14385..14843
                     /locus_tag="PLD99_01300"
     CDS             14385..14843
                     /locus_tag="PLD99_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25573.1"
                     /translation="MPKFRHGEAEDWRDIITLYLIYCFDRINLREIEVAPLHVVLQSV
                     MNHIRRECDLTESDIDMYIGFVYDAGKQRFPAFETSLQKLLEDLVIEQDNDGVRDLIR
                     IIGRGLNTAERNLRKRYPNLADADKSFFLMVCGRAVNNYKTLVNVPNRDD"
     gene            complement(14860..15345)
                     /gene="pth"
                     /locus_tag="PLD99_01305"
     CDS             complement(14860..15345)
                     /gene="pth"
                     /locus_tag="PLD99_01305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRH25574.1"
                     /translation="MYTIIGLGNPGEEYSKTRHNAGKMAVEVLENKEVKAKYIHQDTF
                     MNKSGSAVSKVVKSKKAAEKLIVIYDDLDLPLGTMKISYNRGSGGHKGLESVARAVKT
                     KEFIRIRIGIGKKGDIEKHILGKFTPKELLELKKVFKNLVTAVETIIDDGYEAAMTQF
                     N"
     gene            15376..15951
                     /gene="lepB"
                     /locus_tag="PLD99_01310"
     CDS             15376..15951
                     /gene="lepB"
                     /locus_tag="PLD99_01310"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRH25575.1"
                     /translation="MEPIVTNTESKQNFWKELVKLVLVAVVIVVPFRLFIAQPFIVDG
                     ASMDPTFRNSQYLIVDELTYHFNTPERGSVLIFKYPKDPKKFFIKRVIGLPGEEVRII
                     NGEVTIVNTENPNGLKLVEPYVVYKKEDSHSVKLGEGEYFVMGDNRAGSSDSRFWGPV
                     PEKNVVGRPIFRVWPLSLWPGSLEKFTNEKN"
     gene            15938..17053
                     /locus_tag="PLD99_01315"
     CDS             15938..17053
                     /locus_tag="PLD99_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRH25576.1"
                     /translation="MRKTEFDKPLWIAAHFGFSPIDAPSVTPIDIKITKDCEEDVPKN
                     SNQLVCNAPEKAAFIRHYVDKKLASLPHPLAVSWKRGHNYSLELVGYPVGVAEAKLIR
                     TALSVLTEEGYKDMVVEINSIGDKDSIASYERELKNFVAKTCHNLSQELKKIFKDDAF
                     QIAKSGSEEAKGLRKDMPSSIASLTSQSRAQFKEVLDYLDALKIEYRFAPELVGHKNL
                     CSETLFAIRDAENNLLAGGFRYSRLSRRFGFKRELPLIGATIYGGKKVKGVKIYKEAP
                     KSKFYLIHLGRDARMKALNLIEHLRGYRIPIHHYLGKDKLVAQMQMPEATKSPYHLIV
                     GQKEALDNTVTVRNTNTRAQDTISMDHLTEYLKHLPL"
     gene            17105..17356
                     /locus_tag="PLD99_01320"
     CDS             17105..17356
                     /locus_tag="PLD99_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25577.1"
                     /translation="MINIEVTKGSNENNLSVLRRFQKRVQNAGILVKVREKRYTERTK
                     SENVKRAKTLSYLKRKEEVAELMKLGKMNEVQKFGKKRR"
     gene            17367..17744
                     /locus_tag="PLD99_01325"
     CDS             17367..17744
                     /locus_tag="PLD99_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HRH25578.1"
                     /translation="MDLSIKNLTRSRAPKLPFEEIKNKILGTKYELSLVFIGSTRSRK
                     LNLTYRGKDKPANVLSFPLTKSSGEIFIDLNKTKTFSTEHLFIHGLLHLKGMQHGGKM
                     ESEEQRLLKLFNGQTHFRRHRHR"
     gene            17707..>18848
                     /gene="ftsA"
                     /locus_tag="PLD99_01330"
     CDS             17707..>18848
                     /gene="ftsA"
                     /locus_tag="PLD99_01330"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRH25579.1"
                     /translation="MAKHISVGIDIGSLNIKVVIAEEVRDGARSVQKIIGTGLAETRG
                     IDKGLIVSIPEVARCVQMAVSKAEKQANVEVKRAYVSVSGAGLGSAVATGSVAVSRAD
                     LEVTALDIENAHEAAENSIPEALSINRKIINSIPLETKLDGKPALTRVEGMRGSKLEV
                     KMLFITCLEHHLEDLIKSVEEAGVEVVDVVAGPIASSFVILSKKQKRAGCLLLNIGAE
                     TTSMVVFENSNPHSLEVFDVGGADLTNDIALGLKITLEEAESVKLGSYTRAEYPKKKL
                     DDIIGRRLSSIFELVENHLKKISRNQLLPAGVILTGGACAQYGLKDFAESALKLPARV
                     GTIHFGEEEKTSNKDLPWSTAYGLAVVGFNSDNERGSVGQQGLDKFA"
BASE COUNT         5111 a         4705 c         4300 g         4632 t
ORIGIN      
        1 cagcatcccg acaagccctc cccttgtcgg gttttgttgt ttagattcga tgcggttttg
       61 acttgacttt tggatgattt tgtgatatcg tctaggttag aagctcattg taactactac
      121 atccacgcga catagtcgca aggagacaaa ttgttaacgg cattgttggt attactcatc
      181 ctcacggcct ggttcctggc agcaggactc tggttcgtga agagcggaac gatcgtcgcg
      241 gtactcaggt ttgggcgatt cgacagagtc gcccggccag gaccgaacat catcatcccg
      301 atcttcgaga gtggtgagag gttctcgacc cagacccatc agacggagta ccccctacct
      361 gactcgtcag gcaagaagac acccttccgc atcttgcaaa aggggttgag agaagccaag
      421 ttctacaagc tgaaggatcc caaccttgat ccctcagacc cggaatcata tgaattcgtt
      481 tatgttgacg agttcatcaa ggagaacggg gtgcagaaca ctacattgct cgccgcaatg
      541 gagaacgacc cattgcactc cccgctcacg agcgaggtgg cagtggtcgc agagtggaac
      601 ctgtacgcgg aaatgatccg agtgcaacgc ttcatcgaga acatcgagcc aagagacggt
      661 cgtaccagag aggaagaagt ggacaagcgc atcgaagacg tgatcgccaa gactctccaa
      721 gagttccttg gcgagatcac tgtcggccac gcgcgagacc ggttccctct attgaatggc
      781 ctgatcaaac tacggcttga agagcttgtt ggtgaacatc tcacgaacgg cgtgcctgat
      841 gaaaaggctt ggggcatcaa tgtcaccaag ggttacatca aaaacatcga ccccggccac
      901 acagtgaaca agcagaggtc tgaagctgct ggctcagtca gtgagatgca gaagacgatc
      961 aacgcagcag aagctgaggc cgttgccacg gtcaaggctg gcaatgctga agccaagaag
     1021 atcgaagctg tcggcaaggc cacgaaggaa gctaccatca tggctggtga cgctgaggca
     1081 catagaatca gggtcaccgg cgatgcgacg aaggacgcca tgatgaagca gaatgatgcc
     1141 agggcagatg agattgagag ggtggtcaag ccctcagcag cagaccctct catggtcgaa
     1201 gtcgtcaagg ctcacgccta tcgcgacaac ggcaccgtga tcgcagttgg gggcacgcct
     1261 ctcatcaata catctccgaa gtagtcgtac ccagggccaa ccaaagtatc ggttggccct
     1321 ttttcatact taaataactt gacaaaatag tattttatgc atataatata gaaagaacaa
     1381 actgatctct caaccttgga ggaagcatga ccctgactca gtggttcatc ctgacgatcg
     1441 gagtcctcgg actcatcgcc atcgtggtgt acaagagtgg atggagaccg aagaaggctg
     1501 ctgtagcacc tactggagct accgtagtcc ctggcaagaa gtcagagggt tggtggaaga
     1561 agaacttcgg ctcggtgatc atgatcgcgg tcttcggata cgtcgcatgg gcgatgtacg
     1621 cccacggtga ccgagtggtc aagagcaccg acagcacccg agcactcgcg aacgctctcg
     1681 ccgaagcgaa ccagatgaag aaggacgcga gcaagctctt cgtcgacacc ttggtcatct
     1741 tcgcaacgga agaaggttca cccacaatcg agatccccaa gggagcgaaa gtgcgcttcg
     1801 agcggatgga ggatgtctcc tacgctctcc actacgagac cgagaatgga aggactggta
     1861 ccgtcgagtt ccgtaaggag ctcgagagca tcatctctct cccacccgac ctgaagaagg
     1921 tcgagttcct ctcactcgag aagagctcgg tcaggatcat cttcatgtcg acaaagccac
     1981 tcgaacaccg agtgaccccg tacgaaggcc cccgcatcat tgcgggggct gttttctatc
     2041 tacatttggc ggttaggtac ggtatttaga tattgcggac tagatattcc gaaggtgttg
     2101 agcacaatcc tcataagccc gtaagcagaa aacatgacaa acatccctat caatccataa
     2161 agaatcttct tcttcccctc ctctctatcc ttactctccc cagctttaga tatgaactgg
     2221 tatagacccc agaagaacac taggaaagct atggcgaaga ctaggacaat gatcggattg
     2281 atgatgacag cattaacact gtttaagaac cgagagatgt ccatccgtga gagattagag
     2341 tcccggaaca gtaggaacat tagcactgcc gcctgtgtcc acatttaagt tgtctgcaat
     2401 gaagccaaca agaccccaaa cagcaaccat aacaaagaga cctataaggc cgtagatcat
     2461 gatcctctta ccatcctcct tgttatcctc attgcctgcg gcaaagatga acttcatcaa
     2521 accccagaag aatgcaagta gagcaagtcc aacaacaact ggtaacgcag tatcgactaa
     2581 tctactaatt gagtctagga gagtattaac tccaccaaga gtctgtgcaa aggctacgct
     2641 tggaccaagt gctaatgctg tgtaaagtaa ctttttcatg tatagctttt aaacttaaat
     2701 taattgtaat gtaccgggac aggtacctca taatgatacc tcatatcctc gaactcataa
     2761 tcgagtactc cacaggtctt gttaaatcgt ctggatctgg ataattccag cacctggatc
     2821 aatattcaaa tccctttgga aaaaacctat aatcccccac actgagaaca taacaaagag
     2881 tgcgaccaaa ccgtagacca tcaccttctt accctcgatc ttagattgtt cattgccttg
     2941 ggagaaaatg tatttagcca agccccagaa gaaaccaaga agtgctaggc cggctactat
     3001 agggatagcg agttctacta gattaccgaa ttcaattatc aatgttctta agttttgcat
     3061 caatatattt tattacactc tgccattctc cggtagtaga gggaacccca tctgccccca
     3121 gccgaaacca ctataaaaga aagtgagtat cccccatatg gagaccatta cgccaagacc
     3181 aacaagtccc catttcatca tctccttacc ctcctcgaga ctcttagtat cgtctgactt
     3241 gatgatgaag ttaagcaacc ctctaaagaa cataagaaga gagagacctg caagaaccaa
     3301 aataagaggg ttaatcaacc cgagaaagat gtttaccagg cttctaagat ctgtaggcat
     3361 tatgctctaa gtgagttaac tgtgttctct atgacagtag tgagcacctt cgcccctaga
     3421 agaatggctg ctccgatagc tgtataggta agagcggctt ttgctgtttt aagctcccca
     3481 tcattaccct gcgccatgac gaatttaaat ccagagtaga tgaatgctaa gactaccaag
     3541 atgcctccaa ttggtaggag cactctgtct acaatagtat tcacaaacat aaataggttg
     3601 cagttacctc ctgctcctcc acagttaaat gggtttggta ttgagccact accaccagac
     3661 acggtctcgc cccccctctg tgcactaacg aaagtaacac tgatgaaaaa gactaggatg
     3721 aaagcgaata tatgtggaga aaatttcttc atatttaaat gatactacta tttaggaact
     3781 ctgggctaag taacacgctg gtaatagtct tcactatcac ccatgcggca aggacgaaaa
     3841 aaagaccttt aaggacatta actcctgcat ctttggcctt tttcattgcg ccagcatcac
     3901 cttgagaagt taagagttgg aaaccgaccc aaataaaggc gatagaagca ccgagaatag
     3961 ctataacagt gagattgttt atcaccacat tcgcaagctc aatcaaatcg tcgaaaccac
     4021 aaggatcgct cgtaccgagt gctccgtcac acactatcaa tggcttccag attcggtcca
     4081 tgaattattt ttctaacaag ttattaaggg cggtgttcgc agcactcacg gcttcgtcta
     4141 ctcgagcacg accagaggtg acagactcaa ccatgtcgac aaagatcttc ctcgttgcct
     4201 taggttctgg gtcacgccac caatcagatt gtaacgccgc tccatagaag gtgaaccccg
     4261 caggatctgt aggcggttga gcaagcaaat ctctccttgc tggtacgact ggtgtaatag
     4321 aagtaagtgc actagcagac tccttcgata ctaaggcaag tgccccggca agagcaccgg
     4381 cggggtctct agtcgagcgt actagagcaa taccgtaaag cttgccagaa gtgatgctct
     4441 tacctgaggc tcttgattga ggtactggag agacgcctat gtccatagta gggttcttgg
     4501 ctttgagagt tggcagctcg cttgcgaagc cgagatacat agcgctcttg ccactcgtga
     4561 agtggttctc tgcagatact tgagagcggt tccaagaata atatgacttc tgtgggttcg
     4621 cgaactgagt gtagaactct agtgcggaca aacttggtat ttgagcacca ccattactct
     4681 caagtagagt tgagcgcaag ttctctcctg caaatgcagt gataggcgtc cccgcttgaa
     4741 gtaagagtag ggtcatgatc tccttcacat gaggaatatt cttggcctcg ccgagtgcca
     4801 tcgcagactg agttatgtta cctgcaccat ctttgttggt tagcttggtt atatatgtgt
     4861 aaatctgatc ccagtacacc ggagggacgg cgagcgatgc cctggcaaac atctctctgt
     4921 tccaatacat aaccattggg tcaacataga gaggaagtgc gtaagtaccg agagttgtct
     4981 ggaagagttt ggcctcagag ataaatgtat ttgcgaagtc tgctggtctt acactctcgt
     5041 ttggtataag catgagcttc tcgctctcac tcatgatgct atccacccca aggatgatca
     5101 agtccggtcc cttaccaact gcgagcgcat cagtaaaatc ttttgaaagt gtactctctg
     5161 ccttctcaac atagttataa gagaaagtgc catccttcga gagtccagca gtagacacta
     5221 ggtcagagaa gtcgtaagcc gataatgaac cccagatagt aatattggtc ttagatgtac
     5281 cactacctct gttctgagag aagagtatga ctgcgataac aatgaaggct ccgaaaacaa
     5341 ctaagagcac tatttggaat ttcgacattt gattctattg tagaggtttc ttaaagatta
     5401 gaccatagtg cttctcccct gcattgaagc tcctctcgaa gacgaaccca gctttttcga
     5461 agaacttttt tgtatccact tcggggatgg ccttaccttg gagataatct ttcttacctg
     5521 tgagcatagt gagatccgtc cactcaacga cacaggcctt acctcctggt ttaagcactc
     5581 tctttgcttc ctctagtgca gtaggaatgc tgtttagctg aaacaaaata ttagagaaga
     5641 ccacgccgtc tgcataagcg tcacgtagcc ttactccgcc caacttctca atattgccgt
     5701 ctaacacatc tacattagtc tgaccgagct ccatagattc gttctttaac tttgaaagca
     5761 ggggcttgtc tatatcaata gcgacaacct tacccgcctc acctagggca tgtgatagtg
     5821 cgagcgagta gtgccctgca ccgctaccca agtcgaccac tctctgaccg gggataaagc
     5881 cgaattctaa aacattcttc tttggatcag taaacatgag ctttattata gcaccaatct
     5941 tatccacatt tgacaaatag taaaaaatgg attattgtat aaaaagagtt gttccccgca
     6001 gtacagttca aaaccttaag aaaggacagg ttcgtgatgt gttccatgac ggaactccgg
     6061 gattccgcca agaccaagaa gtctggcacc attctgctcc cctcaactgt gaagcagatc
     6121 cccactgtcg taaagaaggg ggatcacctc ttctacgtcg aaggtaacgg caaactccga
     6181 ttcgcggagg acctcgaccc catgcccatc tccgagctcc cgctctccca gatcccgccg
     6241 aggttgctca ccttggtggc aggcaagcaa cttgcggggg agaagatcat cctttacgcg
     6301 cggaactaac catccgccca caggctcctt agggagcctt ttttctacct gagacgcttt
     6361 acaaattttc atatcggggt atgatactct gcaggatgat cgaacaaagt cgatcattgc
     6421 ttgttccttc cacacacgga gtgtgcgcat gaagttgaag ctcctgcccc tcgcgctgat
     6481 cgccgtttcc ctcgccatgc cgcccaccgc ggatgccgag atcacgaagt tcaacaacgg
     6541 cttcatcgac cagtcccgcg cggcgccggc cgactacacc ctcgaggtgt acgcccggca
     6601 gctcgacttc cccagcttca tcctcacctc caacccggcg atgcccgacc tcatcatggt
     6661 cggtctctcc gagcatcgcg cgtacaacgc ctcccgtctc gccgccacca tggtcgtcgt
     6721 cgaccaggag gtgagccctt cgggcgatct cgcggtcatg atcacctcgt ccacccgtct
     6781 gcttacgcgg agcaagaacc agacgacggc gatcgacgcg cgaagtacca cgcggtaccc
     6841 ggccacggcg acgaacaccg acaaggcgat gaccctcgtc gatctcggca tcgtgaaccg
     6901 atgttgatga ccaagccctc cgagtgagct gacgctcacc aagaggcatc cagcccagac
     6961 aacgatacac tgttgtctgg gctgactctt tttatactag tttttaaggc ttgacatttt
     7021 gttaatttgg gtttattatg tagttagtat tggtcgaatt cctcgaccaa ctctgtaccc
     7081 ctcatcaaaa ggagaagtca gtgaagatcg tggattactt gaggatcgtg gggttctgct
     7141 tcgcactgat cgctgcccca ctcgtccagt tcctctccgt tgctgtcgtc ctcgggatgg
     7201 tcgccgtctc tgctgaagcg cagcagacca agatccaggt cgtcgttccg gtgatcgaga
     7261 tcactaggac gaacaccccc gcgagcacga gctacaactc gttcatcatc gatcacaacc
     7321 cgagcgcggc acgaccaaac ttcgaggtga gctccggcga gaaggtcgac atcttcttca
     7381 aggcaagccg ggcaacggcc tgccaggtgg acaacccagc ggacaacgag cccgctaccg
     7441 gcatcacact cgaatcgaac ttctcgatcg gcccccagca cggctggtac ccagcgatgg
     7501 gcaagaccag cacgatcaag ttcagctgca tcgatcgctt catgaagtcg accgaagcgg
     7561 tcgtaacgat ctctcgacga tgatcaacaa gccccgcttg cacagtagtg caagcggggc
     7621 tgatcttttt tatatattca aatttttaca aacaaatgag agatgcaaga gagtcaccgt
     7681 cgcgaagctt cattatccta actccctgag tcgatctacc gagtactggg acttcagcga
     7741 tatcaacacg caccacttga gacttcttag aaatagctat cacctcagac tcctcttcag
     7801 tcacaacctg gctcgccatt acatctccag tcttgtctgt aaccttggat gtcagtatcc
     7861 cactaccacc cctaccttgg atcttatatt catctagctc tgtgcgcttg ccgtaaccgt
     7921 tagctgaaat gacgagcagg tgcgcattct tccaagcact tttaattaca tgtgctccga
     7981 taacctcatc tttcttatca agagaaattg ctctcactcc accagcagtg cgtcccatct
     8041 cacggatgtc agagtctttg aatcgtatag acatgccctc tttggtgacg agagacactg
     8101 tgtcaccctt ctccgcatga cctaccgcaa caagcttgtc tccagtatca aggcgaattg
     8161 caatgatccc gctcgatcgc acatcggtaa atgattttgc agacacacgc ttaccagtac
     8221 cgtccttggt aataagcacg agtgatccct cgatattctt cgcgtctttt ggcattgcga
     8281 ggatcgaggt caccttctcc tcagcactaa ggttaatgaa gttcattata gacttgcctc
     8341 tggtagcacg cttggcctct ggtatgtcat acattttgat ctgatatgcc ttacctttgt
     8401 cggtaaagaa gagtaaatcc gagtgagtag aagccacaag gaagtttgtg acgaagtcct
     8461 cctccttcgt atccaagtcc accactccga ctccaccgcg cttctgtctc ttatactcgt
     8521 gtggatcagt acgcttgatg tatccgccag atgtgagtac tatcgcctgc tcttcgtcag
     8581 gtattaggtc ttccatagag atattcttca ccccaccctt aacaacctta gtacgacgct
     8641 cgtcaccata cttctccgat accttgagta actcctcttt tattaaattg cgaatctttt
     8701 tgtccgaaga taggagatcc ttaagatatg caataagggt ctgtacttcc ttaagctcat
     8761 cctcaatctt cttcctctct aggcctgcca acttctgcag tctcatctca agaatggcat
     8821 ttgcctgaag ttcagagaac ttaaaggtct tcatgagacc tgctctggca tcatcaacag
     8881 tctttgatgc gcgtattaac ttcactatct cgtctatatg atcaagtgcc ttcttgagtc
     8941 cgaggaggat atgctctctg gcctctgctt gtgcaagatc gaaggcactt ctccttctta
     9001 tcacctcgtc tctatgcttc acaaagctct cgagtatacc cttcaaggac atggtttgag
     9061 gcacaccatc cactagtgcc accaagttca catggaaggt atcttcgagt tgggtgtgtt
     9121 tgtacaaagc attcaaaacc ttttgtggct gactaccttg cttcaggtca ataacgatac
     9181 gaatgtcttt agtcgactcg tcacgaagcg ctttgatacc ctcaatcttc ttctctctat
     9241 gtagttcagc gatcttctct ataagagtag acttgttcac tctataagga atctcggtga
     9301 tgataatctg ctcgatcttg ccgtcgtcca cgatctcggc tactccacga gcaagtattc
     9361 ctccgcgacc ggtagcgtat gcatgggcaa tgtctttctg attataaata atgcctccag
     9421 ttgggaagtc gggtcctttc acgaactcga gtaagtcctc ggttgttgca tctggatcat
     9481 tcaagagcat cacagtcgca tcaacaactt cgtttaagtt gtgaggaggg atgttcgtcg
     9541 ccatacccac agcaatacca agtgtaccgt taagcaaaag gtttggtgcg gcactaggca
     9601 agaccacagg ctccttcttg gtaccgtcgt agttaggacg aaagttaaca gtatccttct
     9661 caatgtcacc aagtaactct tgagcgagtt tactcatctt ggcctcggtg tatcggtaag
     9721 cagcagctcc gtctccgtca atagagccaa agttaccctg accaatcact agtgggtaac
     9781 gggtagaaaa ttcttgagcg agcttaacca tcgcgtcata caccgacgca tcgccgtgtg
     9841 ggtgataact tcctagcacc tctccgacta ccgtagcact tttcctcgtc ttcgcagaag
     9901 ctgtaagacc aatctgatgc atcgcgtaga ggatgcgtct gtgcacaggc ttcaacccgt
     9961 cgcggacatc aggcaaagcg cgatcagtaa taactgacat ggcatagtcg atgaaggagt
    10021 ccttcatctc tgtgacgatc gaacgcgaga ctactctctt actgtcttcg gtattatctg
    10081 tcatcactgg ttactagatt cttccaagag ttcttaagcg agccaacaac cttctccaaa
    10141 ctgttttggc tagctgttgc agccagattc actgtaccag tctccttctt caccaccata
    10201 ggcttcgcac cgaacttaac tccagaccaa atagcaaaga tcgagagagt gacaaccgca
    10261 gaagtaagta gagcaaacct tctcttgtgg tgccttggct tggtatgtag ctcgcttaag
    10321 tactttctca tatctttata ttacaattta ttcttcctta agtaccacta tctcaccttc
    10381 tttgccttcg aggtccttct tcttaagcac gagtttatca attgggtcca ccttgtcctc
    10441 tccggcttct ttgataaatt tcttcaagct ctcggcactg taatgcattg ggataataag
    10501 ggcgggctca agcttcacac tcaacttgta tcctgtattt gggtcgagta ctccgtctcc
    10561 tccaacaggt acgaatagta gatcaatgtc ctctaactcc tcaataatac tggagtccaa
    10621 atctgagtta gccaagtcac cgaggaaaca gatattcata ccctctacat tcatcttgta
    10681 tacagtgttg atgaggtcac ccttgccttt agacaaaaat cctttgatcg agacatcctt
    10741 aacctcgtac tctcccggac caaagatgac aaatccagac ttctccgagg tctcactcct
    10801 cttcgaggta aagagtgtaa tgtcggcagc agtcttcgaa gtaggattga tacttaaaga
    10861 gagagatcct tgtgagatct tgaaagaaga ctggccttgg taggtcacta tcatgcctat
    10921 attttagcat atttgaggct aaaaaccagt acatttctct tcatcttaaa cttgacaatg
    10981 cagtcaattt aagacattat acccacagaa cccgaacccc tcatggaggt agtaaatgac
    11041 gaagcccgct gacccgcacg gacagaaggt ggtggagaag gacgaagacg atgtgagctg
    11101 gtacaccgat tggcggtggc tgggcatcat actcttgctg atcttgatgc tcggtttggt
    11161 catcttccgg ccctgggaca agaacaagga agaagtggcc gaggccgtgt cgacgcccga
    11221 gacgaatcag gtcgactcga cttccgtcac ccattcgtct ccaatgatca cgcttccacc
    11281 tgcaccgaag tattgcgatg tgagcgacag cacacagacc ttctgctcga gtgacccgca
    11341 gacgatcgag gtgcttcaga acggcgaact ccgcctcgcg aatctcggtg aagtgcgcat
    11401 ctgcgctcgc aagccgatca agagcatcac atacaaggtg gtgccgaacg gcaagttcga
    11461 cttcacttca tcactcgaca tcgaaaatcc tcgctgcgac aagttctttg tcacgagggg
    11521 tgaccagtga tccaaggaat caagagggct ctctggacca ctatgggtct gacagttctc
    11581 atgcttggca tctcttcggc aataacagtt gcagaggtgc tgagcaagca gattccaaga
    11641 gctgtcaagc tcgatccaat tacggtgcgt aggagtcgat tccgcaccat ctccccggac
    11701 gaatctgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag atgactgtac
    11821 ccccaaagac ccgctagcac acactagcgg gctttttctt acccatttcc aattaactac
    11881 ccttacctaa ccctcctatt ggtagggtta tccacagttt atccccatat ttctaccaag
    11941 gccgggcctc gggtatgatg tgagttttgc aaaaaagtgc ttgacattta aaacatagta
    12001 tgctaaggta tatgtagatc agttcctgcc cgtacgccac cgtggcgtgc gggtgctgcc
    12061 cctcactctc gcctgaatcc agcgaattgg ctacctggcg gtaaaccaat gagcctaggt
    12121 gagacagtga cgggctactc cgctccggtc agactggtgg ccactcggca atcaactcga
    12181 ctcggagctt gcgcagggcg gacgcattcc gcccaaccac agtcgccgac cccacggggg
    12241 tttacaaagg aggtgaaacc atcgaccaac ccgcggagaa tggacaagct taagcttccc
    12301 atgtctccag ggcccgctta caccaacatt gtcccggcaa ggaccggcgt tggaacacac
    12361 agagcagacg aaatcgcggc atagttttac cccgcgggcc ccggacacga tccggggccc
    12421 gcgggagccc ccgccacacg caacccccac aaagggttgt tcttttttta tatttaaaag
    12481 catttacaaa cagctcattt tctgttacct taatggctcg tgtgagttta atagcaactc
    12541 ttggtacgta tccccttcta gcctaggcaa acactcttga ggctgaagga ccgaccaaga
    12601 atcacactaa atgtcaaaag aaaaagttaa gaaggagaag gttgtcgagg tagacaactc
    12661 tcttatgggt cagttggtag ctaagtcacc aaacccacct aaggttgagg acgtggtaga
    12721 gggttctgtc atcagcatcg acaaaggagc ggtgtatatc gacttacctc catacggtac
    12781 cggtatcatc tacggacgcg agttcatgaa cgctcgtgac attatcaaga agatcaatgt
    12841 cggcgacgca gtcgcaggta aggttgtgtc gatgggtggc aaggacggtt acatcgagat
    12901 ttctcttaag gaagcaagac aagcactcat ctggagcgac gctgacgaag caatacgcga
    12961 caagagagtc tttgatattc ttgttactga tgccaataag ggcggactta ttttgaactg
    13021 gcaaggtatc cagggattcc tcccagcatc acagctcaaa cctgagcatt atcctagagt
    13081 taacgatggc gataaggaca agatcttaga cgagctaaga aagctagtcg gagagaaact
    13141 aactgtcact atcatcggtg cagcacctaa ggagggtaag ctcatcttct ctgagaagtc
    13201 acctgagacc aagagcaagg agagtatcct cgctcgttac actctcggcg acgaactcat
    13261 gggtgagatc acgggacttg tcgacttcgg cgtctttgtt aagctcgaag aaggactaga
    13321 gggcctagta cacatctctg agatcgattg gggtctagta gagaatccta aggcaatgtt
    13381 taagattggt gagaaggtga aggtgaagat catcgagatc aaagatgaga aaatctcact
    13441 ctctatcaag gccttgaagg agaatccatg gaaggatgca tcaaagaact acaagaagga
    13501 cgatgttgta gaaggtgtca tcatcaagta caacaagcat ggtgcactcg cttctataga
    13561 ggaaggtgtg gcaggcctag tacatgtctc tgagttcggc tctgaggaca acctaagagc
    13621 gactcttgag atgggtaagt catactcgtt caagatcaca ctctttgatc ctaaggagca
    13681 aaagatggct ctctcgtaca tagacaagag taaaaaataa agtagaaata attctgtttt
    13741 aaagaaatcc ccactcaggt ggggatttct tattatgaat ttaaaatgaa tgttacagtt
    13801 gttgtagata ctgaacttaa caagaggcca aaaatgggcg atcgaatgat tgaagaagac
    13861 aacatcaagg aattgattgt cttctcagta ttggtgtgtc tcatcaggga taaggggtac
    13921 cgaagagatt cagtctcggt cctcaagcta cacgagtgtt ggcacctcat taaggagacg
    13981 aagatcgaag gtgtcaatgt caactggttc agaaagaatc ctgtcacacg tatttgggaa
    14041 gagctcgacc gtgccatcca gaggcttgta tttagccatt ggatcgagcg cggcaaaatc
    14101 tatcgatcga gcactcttag tctgttgact ggcaccacga ccgaaaggaa ggttttcact
    14161 gagtattggc gaatctattc ggccatactc cagatcttcc ctgatttcgc gagactctat
    14221 gatgccatcg aggaacccgt cgtgatcgac ggtatccgag agatgccgat acacgggctc
    14281 gagaatcaag tttctacccc cgaagcgtag cttcgggttt ttctttgcat aaatacataa
    14341 tttgtggcta tatgggttca gattgaaccc caaacggagg caatatgccc aagttccgcc
    14401 atggagaagc cgaggactgg agagatatca ttactctcta cctcatctac tgcttcgatc
    14461 gcatcaacct gcgtgagatc gaggtcgccc cactacacgt agtcttgcag agcgtgatga
    14521 accatattcg aagagaatgt gatctcacag agtcagatat cgacatgtac atcggcttcg
    14581 tctatgatgc gggcaagcag cgattcccag catttgagac ttcgcttcaa aaactcttgg
    14641 aagacctcgt tatcgaacag gataacgatg gagtgcgaga cttgatcagg atcatcggtc
    14701 gtggactaaa caccgccgag aggaacttga gaaagcgcta tccgaatctc gccgatgcgg
    14761 acaagagctt cttcctcatg gtctgcggtc gagcagtcaa caactacaaa actttggtga
    14821 atgtaccaaa ccgcgacgat taggcgcggt ttttcttaac taattaaact gagtcattgc
    14881 tgcctcgtat ccatcatcaa ttattgtctc tacagcagtt accaaattct tgaagacctt
    14941 cttaagctcg agtaattcct ttggggtgaa cttacctaag atatgtttct ctatatcacc
    15001 cttcttgcct ataccaatac ggatccttat gaactccttg gtcttcactg cccttgcaac
    15061 agactctaga cccttgtgtc caccagaacc tcggttataa gaaatcttca tagtgccgag
    15121 tggtaggtcc aaatcatcat aaataacaat caatttctct gcagccttct tacttttgac
    15181 taccttagac accgcacttc ccgatttatt catgaaggtg tcttgatgaa tgtatttggc
    15241 cttaacttct ttgttttcta ggacctcaac agccattttg ccagcattgt gacgagtctt
    15301 ggagtactcc tcacccggat ttcctaggcc aattattgtg tacatgaaaa gattataata
    15361 caatgtctcg aaattatgga accgattgtc actaatacag agtccaaaca gaatttttgg
    15421 aaagaattag tgaaattggt gctcgttgca gttgtaatag tcgtcccctt ccgtctcttc
    15481 atcgctcaac cattcattgt tgatggtgcc tcgatggatc caaccttcag gaacagccaa
    15541 tacttgatag tcgacgagct tacttaccat ttcaacaccc cagagagagg ctcggtgctc
    15601 atcttcaagt acccaaaaga tccaaaaaag tttttcatca agagagtaat aggtttaccg
    15661 ggtgaagaag taaggatcat taatggagaa gtaactatcg tgaacacaga gaatccaaat
    15721 gggctaaagt tagttgagcc ttatgttgtg tacaagaagg aagactctca ttcggtcaaa
    15781 ttaggagaag gcgagtactt cgtcatgggt gataaccgcg cggggagctc tgactcaaga
    15841 ttctggggtc ccgtacctga gaaaaatgtt gttggtcgac caatctttcg tgtttggcca
    15901 cttagtctat ggccaggtag cttagaaaaa tttacaaatg agaaaaactg aattcgataa
    15961 accactatgg atagcagcac acttcggctt ctcgccgatc gatgccccgt ccgtcactcc
    16021 aatagatatc aaaattacca aagactgcga ggaagatgta cctaaaaaca gcaatcaact
    16081 agtctgtaac gctccagaga aggccgcctt catcaggcat tacgtagata agaaattggc
    16141 cagtctcccc cacccacttg cagtatcttg gaagcgaggt cacaactact cgctcgagct
    16201 tgtaggttac cccgtgggag tagccgaggc caagcttatt cgtacagcac tctctgtctt
    16261 gaccgaagaa ggttacaagg acatggttgt cgagatcaac tctatcggcg acaaggactc
    16321 aatagcttcg tatgaaagag agttaaagaa tttcgtagcc aagacttgtc acaatctctc
    16381 acaagagtta aagaaaatat ttaaagacga cgccttccaa attgctaagt cggggagcga
    16441 ggaagcgaaa ggacttagga aagacatgcc ctcctctatt gcgagcctca cttctcaaag
    16501 tcgagctcaa tttaaggaag tgctcgacta cctagatgct ttgaagatcg agtaccgctt
    16561 cgccccagaa ttagtcgggc ataaaaatct ttgttctgag accctctttg ccataagaga
    16621 tgcagagaac aatttgctcg ctggtggatt cagatactca agactctcca gacgtttcgg
    16681 cttcaagcga gagctacctc tcattggtgc caccatctat gggggcaaga aagttaaagg
    16741 agtgaaaatt tacaaagaag ctcccaagtc aaaattctat ttaatacacc ttggacgaga
    16801 cgcgaggatg aaggctctta acctcatcga acatttgaga ggctatagga tcccaataca
    16861 tcactacttg ggcaaagata agctagtagc tcagatgcag atgccagagg caaccaagtc
    16921 accctaccac ctcatcgttg ggcaaaagga agccctcgac aatacagtta cagtgagaaa
    16981 taccaacact cgagctcaag acaccatctc catggaccac ctaactgagt acctcaaaca
    17041 cctacctcta tagccatttc agggcttgcc tgagcccaaa ccttgtgcta aaatggccca
    17101 actaatgata aatatcgaag taaccaaagg tagtaacgaa aacaacttaa gcgtcctaag
    17161 gcgcttccaa aagcgtgttc aaaacgcagg tattctagtg aaggttagag agaagcgtta
    17221 caccgaaaga accaagagtg agaatgttaa gcgtgctaaa accctaagct atctaaaaag
    17281 gaaggaggaa gtcgcagagt tgatgaagct cggtaagatg aacgaagttc agaagtttgg
    17341 caagaagcga cgctaatttt atttcgatgg atctctccat caagaatctc acacgttctc
    17401 gagctcccaa gctccccttt gaagaaataa aaaacaaaat tttaggtact aaatacgagc
    17461 tatcgctcgt ttttattggt agtactcgct cgagaaaact caatttgact taccgaggca
    17521 aggacaaacc tgcgaatgta ctctccttcc ctctcactaa aagtagtgga gaaattttca
    17581 tcgatttgaa taaaacaaaa acttttagca ccgaacacct ctttatccac ggcttactac
    17641 atttgaaagg gatgcaacat ggcggtaaga tggagagtga agaacaaaga cttttgaaat
    17701 tatttaatgg ccaaacacat ttccgtcggc atcgacatag gtagtttaaa tattaaggtt
    17761 gttatagccg aggaagttcg tgatggagca agaagtgtgc agaagattat cggcaccgga
    17821 ctcgccgaaa ctcgtggtat agataaggga ttaattgtaa gcatccctga ggtagctaga
    17881 tgtgtacaga tggcggtgag caaggcagag aagcaagcca atgtcgaggt caagcgagcg
    17941 tacgtgtcag tcagtggcgc aggactaggc agtgctgttg ctactggctc ggtagcagtc
    18001 tcaagagcag acctagaggt cacagcacta gacattgaga acgcccatga ggccgcagag
    18061 aactctatcc ccgaagcact ttcgatcaac aggaaaatca tcaacagcat cccgctcgaa
    18121 acgaaactag acggcaagcc agcactcact cgtgtagagg gtatgcgtgg gagtaagctt
    18181 gaggttaaaa tgctttttat cacctgtctt gagcatcatc tagaagattt aattaaatca
    18241 gttgaggaag caggggtcga agttgtcgat gtcgtcgctg gacccatcgc ctcatcattc
    18301 gttatccttt caaagaagca gaagcgcgct ggttgcctcc ttcttaatat cggcgccgag
    18361 acaacctcca tggttgtgtt tgaaaattct aatcctcact cactcgaggt ctttgatgta
    18421 gggggtgctg acctcacaaa tgatattgct cttggactaa agatcactct cgaagaagca
    18481 gagtctgtaa aacttggttc ctacacccgt gccgagtatc ccaagaagaa gttagacgac
    18541 attattggta ggaggctctc aagcattttc gaactagtgg agaatcactt aaagaagatc
    18601 agcaggaacc aactcctccc agcaggagta atcctcaccg gtggtgcatg tgcacaatac
    18661 ggtctcaagg acttcgccga gagtgctctc aaactccctg ctcgagtcgg cacaatacat
    18721 tttggcgagg aggagaagac ttcgaacaaa gatctgcctt ggtccacagc gtacggactt
    18781 gctgtagttg gcttcaactc agacaacgaa cgaggttctg tcggccaaca aggtttggat
    18841 aaatttgc
//
LOCUS       DAORIT010000009        18619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_486_length_18619_cov_53.639733, whole genome shotgun sequence.
ACCESSION   DAORIT010000009 DAORIT010000000
VERSION     DAORIT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18619
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_486_length_18619_cov_53.639733"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLD99_01335"
     CDS             complement(<1..649)
                     /locus_tag="PLD99_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25580.1"
                     /translation="MADKKEAPKTDPVVEVGFIILVLIFLWVIWSTILNYLLVSRFGS
                     YSTMWEAVATWFIRYIYPIIILLGLVATILAIMSMVRNMSKLKALKEAEKKIFGSDPD
                     GVLVKEVVAPLNPKWEKAMSHLNSTNASEWRLAIIEADIMLDDMLRAQGHHGDSIGEM
                     LKGIERSDMLTLDFAWEAHKIRNEVVHSGSTYELNEREARRVMSLFEAVFREFKMI"
     gene            complement(659..>2044)
                     /gene="gatA"
                     /locus_tag="PLD99_01340"
     CDS             complement(659..>2044)
                     /gene="gatA"
                     /locus_tag="PLD99_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRH25581.1"
                     /translation="KLTVVSAREALKKKEFSAVDLTKRSLEVIKEKDGELHAFLEVFD
                     DVLDQAEVADKKIGEGIDLPLLGIPVAIKDNILFKGHIASAGSKILENYRATYDSTLI
                     KKLKEAGAIIIGRTNCDEFAMGSSTENSAYGITKNPVDTTRVPGGSSGGSAVVVAAGM
                     VLASFGSDTGGSIRQPAAFTGIVGMKPTYGRVSRSGLIAMASSLDQIGPFTKTVGDAE
                     LLYEISKGRDDMDSTTISDDTYEIRGNKKVIGVPRSFFESSLDKEVMENFNLAVEKFK
                     KLGYEVRDIELPHLKYSLAVYYILMPAEVSSNLARFDGVKYGLHVDGENSIDDYFKTR
                     GQGFGREARRRVILGTYILSSGYYDAYYGQAQNMKKVITDEIREAFKSVDVILTPTTP
                     TPAFKFGEKSDPLSMYLADIFTVPANITGCPSISVPSGSVDGLPLGVLITGDLGSEEK
                     LFATAKDFEKS"
     assembly_gap    2045..2144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2145..3595)
                     /gene="ligA"
                     /locus_tag="PLD99_01345"
     CDS             complement(<2145..3595)
                     /gene="ligA"
                     /locus_tag="PLD99_01345"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRH25582.1"
                     /translation="MKPVPKEVKERVDKLRKTIDRYRYDFHVLNLESISPEALDSLKD
                     ELTKIERDYPELVTPDSPTQRVAGAPSAGFKKITHKVSQWSFNDAFTVEDILEFNERV
                     KRFYKTETGEDIEPVYTCELKIDGSHLVLEYENGILISGATRGDGKVGEDITSNVRTI
                     ESVPLRLSEDVSVIVEGEVYMPKSQFEKINNEQRRRGEKEFANARNIVAGTVRQLDSK
                     LVAERGLSTFIYDVSKADNLPKTQEEELDRLEVLGFKVNKHHKVANNIDEVVEYWKLW
                     SKKKDKEDYLIDGVVVKLNDRRAQEILGYTGKAPRWGIALKFEAEQVTTVVEDITLQI
                     GRTGVLTPVANLRPVRVAGSTVSRATLHNEDEIQRLDVRVGDTVIIQKAGDVIPDVVS
                     VVVDLRPKNSKPFKFPTHFPLCGGDGRIERIPGQAAHRCVAKNSFAQQRRKLAHFTSK
                     HCFDIAGFGQKTVDQLLEAGLISTFEDIFRIKRG"
     gene            complement(3620..4075)
                     /locus_tag="PLD99_01350"
     CDS             complement(3620..4075)
                     /locus_tag="PLD99_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HRH25583.1"
                     /translation="MKNRGFTLFIAIVISATLLLVSSGIISVAVKEAFLTTANRHSQE
                     AFYAADTGIECALYWDLKNPGGLSAFATSTTAVISCNGQSFTVGGSSGQSNFTLTFLP
                     DLYCTEVRVTRRRAQPSLNIKSTTIESYGYNTCDPTNPRRVQRAIGVSY"
     gene            complement(4072..>4606)
                     /locus_tag="PLD99_01355"
     CDS             complement(4072..>4606)
                     /locus_tag="PLD99_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRH25584.1"
                     /translation="QNLQTNKKGFTLVETLIAITILVLAVTGAFAAAHNGMMSSLYSK
                     DQVTAFYLAQEAIEQIRNIRDQNGIRGNTQGWLAGIANSGDPCAVGNTCYVDALSSSA
                     PLIQCPATCPNLLLNSNGFYSYDNGTTTKFRREVTVTSVPGSNGNEVVIQAKVFWSKG
                     VLVDRSFTATEHLFNWQ"
     assembly_gap    4607..4706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4707..4933)
                     /locus_tag="PLD99_01360"
     CDS             complement(<4707..4933)
                     /locus_tag="PLD99_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRH25585.1"
                     /translation="MMKKGFTLIEIMTAVSIFAVITTISMGSILAVFDVNRKSESIKT
                     VMDNLNLAIESMAREMRFGTNYICSPLLPML"
     gene            complement(4930..5613)
                     /locus_tag="PLD99_01365"
     CDS             complement(4930..5613)
                     /locus_tag="PLD99_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25586.1"
                     /translation="MFKHSSIKGSRGLTVMELLVSIAIMIIISTVIASNQKQFGAGAA
                     LKNIVNNLSLSLRQAQIYGVSVKQLSPSSSQAYTTTGFNAGYGVHFSIVPSYPAGDNG
                     SYLFFLDTKPSALVAPNGLYLGDMNCNPIGGTPASECLDKVVLGQGHTITALCSLESG
                     TENCTNEQLDITFVRPAVDARITFNNTGVLTDTACIEVSATDGRKNSIVVYTTGQVSV
                     RGTSCMDAL"
     gene            complement(5591..6334)
                     /locus_tag="PLD99_01370"
     CDS             complement(5591..6334)
                     /locus_tag="PLD99_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRH25587.1"
                     /translation="MIVFIFILGVIIGSFLNVVGLRFRSGLTLGGRSFCPTCGKTLTW
                     HELIPVVSYILLRGRCSKCKTHISLQYPLIELWTGMVFASFYSVFGLTSYSLLLAIVF
                     SVYTVILIYDLRHKIIPDFLVYLSIGLSLLVPIFIIPYSLNDWLAGPILFSAFGLIWL
                     VSKGRALGFGDAKLVLSIGLLLGAAQGYSATALAFWIGTAVTLPIMLFSRKGITMKSE
                     IPFAPFLIIGAWVSVFFGFDLFHVQTLFN"
     gene            complement(6347..6820)
                     /locus_tag="PLD99_01375"
     CDS             complement(6347..6820)
                     /locus_tag="PLD99_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRH25588.1"
                     /translation="MNKTNQQGFTLIELLVVIAIIGILSSVVLASLNSARTKANNSKV
                     KAQLSSIRASAELFYDSNNSAYIASNMGTEQPACTGTMFFDASSPVSTITGTSQVWPG
                     NVLLSCQATDSAYAVSASLPVAEGVGGAITHWCVDSAGRSMGITSALASNDVSCN"
     gene            complement(6851..7354)
                     /locus_tag="PLD99_01380"
     CDS             complement(6851..7354)
                     /locus_tag="PLD99_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRH25589.1"
                     /translation="MLNTSKKGFTLIELLVVIAIIGVLSSVVLASLNSARNKANNSKV
                     KAQLATVRAAAELYYDTNNSSYAPAVTGMVSAPACTAGAPVNGGGMFVDVNSGMNALT
                     GTAGSWPTNTSMRCYSTAQAYAVSASLPIAEVVGANTYTLWCVDSGGRSMGRTAVQGH
                     VTAATCL"
     gene            complement(7348..7803)
                     /locus_tag="PLD99_01385"
     CDS             complement(7348..7803)
                     /locus_tag="PLD99_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRH25590.1"
                     /translation="MLKRTTKGFTLIELLVVIAIIGILSSVVLASLNAARGKSQNAKI
                     KAQLANARSSAEIYYDNNGSTYNGIAGDVTGDCTTADSMFVDTGSNMASFTDPANYPN
                     NPTITCYSTLDAYAVSAPLYVDDNGNNEWCVDSTGTSMGRVGPITSTSC"
     gene            complement(7928..8398)
                     /locus_tag="PLD99_01390"
     CDS             complement(7928..8398)
                     /locus_tag="PLD99_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRH25591.1"
                     /translation="MLKRTTKGFTLIELLVVIAIIGILSSVVLASLNSARTKANDSKV
                     KAQLATLRASAELYYDNNNSSYGAVVAMAAPASVCAGSGTMFQDTTSGMANLTNNASA
                     WPSGTLLSCQVTATSPVGYAVSANLSTAGTFWCVDSSGKSASTSAAQASGDITC"
     gene            complement(8442..9650)
                     /locus_tag="PLD99_01395"
     CDS             complement(8442..9650)
                     /locus_tag="PLD99_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRH25592.1"
                     /translation="MLYNYKALEQTGVQKEGSIDAVSLEVAIDSLQKRGLIIASIDEA
                     DKEPWYSKLTIGAKVKNKDIVILSRQIATLFGASVSALKIFSLLSGEIENIHLRKSMV
                     EIADDLQAGSSISKALSKHPDIFSDFYVNMVKAGEETGKLNETFDYLADHLDRNYEVV
                     SKAKNALIYPIFVIGVFVAVMVLMFTLIIPKISVILLDSGQPVPVYTAIVFAISDFII
                     DYGVILLVFLVIVAFVLIRYLNTQDGKRALGRFKLEVPYVGDLYQKLYLSVISDNLAT
                     MILSGIPMVRAIEVTANVVGNDIYKTILSDSGVAVKSGSSLSQSLMQYEEVPSILTQV
                     IKVGEESGELGEILKTMAKFYQREVINAVDTLVSLIEPVMIVALGLGVGTLLASVLIP
                     IYNIANTAGV"
     gene            complement(9659..10723)
                     /locus_tag="PLD99_01400"
     CDS             complement(9659..10723)
                     /locus_tag="PLD99_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRH25593.1"
                     /translation="MAASHSLLDELVDIVNKEGASDLHIAEGRKPIIRVSSFLIPLEK
                     YPAMTADDVKSFFNIFLTPENMKEFEMKQEANFAFSHKSGARFRGNAFETVGKRAIAL
                     RLIPTRIRTFEELNLPKILSHFTSKQQGFFLVVGPAGQGKSTTLASMVELINNDRLEH
                     IVTIEDPIEFLFEPKKSMIDQREVKTDTPDFHTALWGIFRQDADVIMVGEMREPHTIA
                     TAVTAAETGHLVLSTLHTNTAAQTVDRIIDSFPAESQDQIRYQLSSSLLGIFSQRLIP
                     RIKGGLIPAYELLINNTAVSNLIREKRTHELNSIIETSSEDGMIDFNRSLSELVRLGE
                     ITPENAFQYSTNPRSLERMI"
     gene            complement(10727..11113)
                     /locus_tag="PLD99_01405"
     CDS             complement(10727..11113)
                     /locus_tag="PLD99_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRH25594.1"
                     /translation="MKKPYTVLLVDDDKFLLGMYQKKFDKLGAKAEMCAVPEEALNKL
                     REGAKYDIVVLDVIMPGMDGLELLKKIREEKVAESSIFIMLTNENTSARIEEARSLGV
                     AGYIVKATSIPSEVAEEIIKIADGQN"
     gene            complement(11121..12845)
                     /locus_tag="PLD99_01410"
     CDS             complement(11121..12845)
                     /locus_tag="PLD99_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRH25595.1"
                     /translation="MTILDVLKDKKVIKATDVASIEEEAKSTGLSLEQVLVGRGVPPQ
                     KILEAKGAELEIPTKSLKNVSVPSKMLEYISEESAEHYKVVPIGIKDGVLEIGVVDPD
                     DIEARDALNFISSKAGMPYKLFLISQEDFERIFAQYKGLSSEVTQALSELDSELTQRE
                     NVILEKTDKEKEAQQDREAKIVEDAPVTKIVATILSYAAEGAASDVHIEPLHETVRVR
                     FRVDGVLNTSLTLPIKVHSAVVARIKILSNMRLDEKRKPQDGRFSAKVDDRRIDFRVS
                     TFPTTFGEKVVMRILDQSKGVKTLKDLGLSDENMKLIKDAIDRPYGLILISGPTGSGK
                     STTLYAMLNELDKDHENVLSLEDPVEYNMEGVSQSQVQPEIDYTFATGLRTTLRQDPD
                     VIMVGEIRDKETAQLAIQAALTGHLVLSTIHTNNAIGVIPRLIDMGVDPYLIAPTLVL
                     AMAQRLVSKLVEGSGKPVPVSESMKLMMEKQFADLPAEFRKKIPTGKEFYEAAPTPES
                     VTGVSGRVAVMEVLSMDKDIEQVLLKNPTEIELFKMARSKGMLTMKEDAIIKAFNRII
                     PFEEVNTL"
     gene            complement(12857..13156)
                     /locus_tag="PLD99_01415"
     CDS             complement(12857..13156)
                     /locus_tag="PLD99_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25596.1"
                     /translation="MDFFHKNKNIILILVLLACGFYFYSAYFKPDSQIVVTPDDLTAQ
                     AVGAEVIELQSKITSVNLKQGLFTSSLYNKLIDFSVPILEQPRGRSNPFSPVGFD"
     gene            complement(13165..13713)
                     /gene="pilO"
                     /locus_tag="PLD99_01420"
     CDS             complement(13165..13713)
                     /gene="pilO"
                     /locus_tag="PLD99_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRH25597.1"
                     /translation="MKNSTALILLLISVALVYTVGFPYYDKIMILRTQSAQYKNSLDQ
                     VDILIKKRNDLEAKYQNMPQNEIAKLEKVLPDNVDTVNLAVNFDSIAAKYGISIKKIS
                     TVDQVDQSGAAIVQDSSGGSYKPVTVIFSFTAPYEDFRDFIGDIEQSLRITDIKSVSF
                     ESNDDGLYEYSLAVQTYWLKTE"
     gene            complement(13719..14387)
                     /locus_tag="PLD99_01425"
     CDS             complement(13719..14387)
                     /locus_tag="PLD99_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25598.1"
                     /translation="MEPKFQSSFIPKGPLATTGTATKTSRQSDTSFLGTLAVFVFALA
                     ILATLSAVGYEYYLKKDIGSLAKKLLDAKSTLQPEVIKKISDLDERLKATESLLNNHI
                     VLSPLFEYLETETVKTIQLTQFKYTSEEGVLKLTMRGKSRGYSTLAFQAQRFNASDFM
                     KDVVFGDLSLDNTGNVDFTFKATLDPSIIAFNKGDEPEAVVEAPVEAGVSVEEPVTTN
                     GTTQ"
     gene            complement(14441..15544)
                     /gene="pilM"
                     /locus_tag="PLD99_01430"
     CDS             complement(14441..15544)
                     /gene="pilM"
                     /locus_tag="PLD99_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRH25599.1"
                     /translation="MAFLGKFFGKKESPSVVGIDIGSSAIKVVQIKKKGGQAVLETYG
                     ELALGPYGGASIGQAVNLPQEKVASALFDLMREKEVNITTTQAGISIPFASSLMSVIE
                     MPEVSQKQLEVMVPIEARKYIPVPVNEVMLDWSVIPKPSNPNEDEDDEEKKNALKRID
                     VLVVAIHNDTIARYQNIIQKAGLEPSFFEIEIFSTMRSVVDESLTPVMVMDMGAASTK
                     LYIIERGIIRASHTVNRGAQDITSAIAQSLGLSIEKAEIMKREIGASGQDPTLAKIIS
                     LVLDFIFAETNRTLLNFEKRYNKSVSKVVLVGGGAAMKGLNELTKNAFKTEAVMGNPF
                     GKLATPAFIENILKETGPEFAVAIGAALRRLND"
     gene            15564..16208
                     /locus_tag="PLD99_01435"
     CDS             15564..16208
                     /locus_tag="PLD99_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRH25600.1"
                     /translation="MFRALISNLFDFLYPKDTSVISLESLKPEELLTKLTTAKSLSND
                     TFAIWNYADEKVRTIIWEIKYRRNEVLLKNVGQVLYDTVSSEVLERMVTENFVRPLLL
                     PIPISKRRRLERGYNQTELLCKVMKKLDTENLFEYSPNALRRVIHTESQARTKNKKER
                     ITNMQNTIEANESTKDRNIILIDDVTTTGATLGDARRALRESGAKRIICFALAH"
     gene            complement(16225..16629)
                     /locus_tag="PLD99_01440"
     CDS             complement(16225..16629)
                     /locus_tag="PLD99_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25601.1"
                     /translation="MPRNLAIAVVAIALIGGGYLLKNSDAPKVVTNTDANLALVSFEP
                     RTGEIDATGVLDCTPHKSGKPVAEGECVMGLKADDGKFYSLDTSKMEDAKGSNLEKVR
                     VLGTFVATDAENPEVGDYAYDGVITVRAIEAN"
     gene            complement(16631..17077)
                     /locus_tag="PLD99_01445"
     CDS             complement(16631..17077)
                     /locus_tag="PLD99_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25602.1"
                     /translation="MDSNMQSAWYKNKMVLGVVLIVVVALGLVLFGGDKAPKTEGGVV
                     GEVTETTNVEPKSGEIAVVGKLGCTPLKSGAQPTNEQCVLGLVGSDGKFYALEYSKLE
                     MAESGIEPNSVVKVVGTYTKVDASSEEATVFKYDGVMAIRLMVINK"
     gene            complement(17163..18056)
                     /locus_tag="PLD99_01450"
     CDS             complement(17163..18056)
                     /locus_tag="PLD99_01450"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRH25603.1"
                     /translation="MQKIIPSLWFNNNCEEVINFYTSIIPNSKITTIKRYPTDFQVGP
                     IPGMGGKVLTAVFELAGEKFMALDGGDYFKFNPSVSFTITCPTVEETEAMYKKLSEGG
                     EDLMPFQKYPWAERYGWFKDKFGLSWQINMEPGVVKIVPSMMFVGKYFGHVEEAIDFY
                     TSVFKNSSKHMVVKYEEGDSDVTGKVKYSSFSLENHPFIAMESSAEHKFEPNGAVSFT
                     IECEDQAETDYYADKLSANPEAEQCGWVQDKYGFSWQVIPRRMGELVTGPDKAGADRA
                     LKAMLEMKRINLAKLEEAYKG"
     gene            complement(18072..18560)
                     /gene="msrA"
                     /locus_tag="PLD99_01455"
     CDS             complement(18072..18560)
                     /gene="msrA"
                     /locus_tag="PLD99_01455"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRH25604.1"
                     /translation="MTKKAVFAGGCFWGLEELIRSQPGVVNTEVGYTGGENDNPTYTN
                     HPGHAEAVEIEYDPTQTTYKKLLDFFFQIHNPTTLNQQGNDIGTSYRSAIFYANEEER
                     EEAEKFIKIVDDSKRWQRPVVTSLEPLTKFYKAEEYHQDYLQKNVGGYTCHAIWFDSY
                     LK"
BASE COUNT         5108 a         4498 c         3666 g         5147 t
ORIGIN      
        1 atatcatctt aaactctcgg aaaacggctt caaagagtga catcactctc ctggcttcgc
       61 gttcattaag ctcataggtg gagcctgagt ggactacctc gtttcttatc ttgtgtgcct
      121 cccaagcaaa gtcgagagtg agcatatctg acctctctat acccttaagc atctcgccta
      181 tagagtcacc atggtgcccc tgagctcgaa gcatatcgtc gagcattata tcggcctcta
      241 tgatagccaa gcgccattcg gaggcattag tcgaatttag gtgactcata gccttctccc
      301 actttgggtt caatggtgct acaacctcct taaccagaac accatctgga tctgagccaa
      361 agatcttctt ctcagcttct ttaagagcct taagcttcga catatttctc accatactca
      421 ttatggccaa aatcgtagcc acaagaccaa gaaggataat aattgggtat atgtatctaa
      481 taaaccaggt tgctacagcc tcccacatag tgctatacga cccgaagcgt gagacgagta
      541 agtaattcaa aatggttgac catatcaccc agaggaatat aagtaccagg ataataaaac
      601 caacttctac gactgggtct gttttaggag cttctttctt gtctgccata cttacattct
      661 aactcttttc gaagtcttta gctgtggcaa aaagcttttc ctcactacct aagtctccgg
      721 taataagcac accaagtggc aaaccatcta cactacccga tggtactgat atcgaggggc
      781 aaccagtaat atttgctggt acggtaaaga tatctgccag gtacattgag agaggatctg
      841 atttctctcc aaatttaaat gctggtgtag gtgtggttgg agtaagtatc acatccacac
      901 ttttaaacgc ttctctgatt tcatctgtta tgactttctt catattctgt gcctgaccat
      961 aataagcatc atagtaacca gaggaaagaa tataagtacc gaggatcact cgcctcctcg
     1021 cctcgcgacc aaagccctgt cccctagttt tgaaataatc atctatagaa ttttctccat
     1081 ctacatgtag cccgtacttt accccatcaa atcgagctag gtttgaggaa acctcggccg
     1141 gcattaagat gtaatacact gcaagcgagt attttaggtg cggtaactcg atatctctaa
     1201 cttcatatcc tagtttctta aacttctcca cggcaaggtt aaagttctcc atcacttcct
     1261 tgtctaaact agattcaaag aacgatcttg gcacacctat tactttctta ttacccctta
     1321 tctcatatgt atcatcgcta atagtagtgc tatccatgtc atctcggccc ttagaaattt
     1381 cgtacaaaag ttctgcatct ccaactgttt tagtaaaagg gccaatttgg tcgagcgagc
     1441 tcgccatagc aatgagtcca cttctagata ctcttccata agttggcttc atccccacta
     1501 tgcccgtaaa ggcagctggc tgccttatag aaccccctgt gtctgacccg aagctcgcga
     1561 gcaccatacc agcagctaca accactgccg aacctcctga ggaaccccct gggactcttg
     1621 ttgtatcaac aggattctta gtgataccgt aagccgagtt ctcggtcgaa gagcccatgg
     1681 caaactcatc acagtttgtc ctaccgataa taattgcccc tgcctccttt agtttcttga
     1741 ttaaggttga gtcgtaagta gcacgataat tctctaatat ctttgatccg gcagaagcaa
     1801 tatgtccctt gaataaaata ttatccttaa tagcaaccgg gatacctaaa agtggtagat
     1861 ctataccctc cccgatcttc ttgtcagcaa cttccgcctg gtctagcaca tcatcaaaca
     1921 cctcaagaaa tgcatggagc tctccatcct tctctttgat aacttcaaga gacctcttgg
     1981 ttaaatcaac tgcagaaaat tccttcttct tcagagcttc tctcgcgctt acaactgtta
     2041 acttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctctc ttaatcctaa
     2161 atatatcttc aaaggttgag ataagtcccg cctcaagtaa ttgatcaact gttttctgcc
     2221 cgaaaccagc aatatcaaaa caatgtttac tagtgaaatg cgcaagcttc ctcctctgct
     2281 gagcaaaaga gttcttggct acacatctgt gagctgcttg gccaggtatc ctctcaatac
     2341 gaccatctcc tccacaaagt gggaagtgag tcgggaattt aaatggcttt gaattcttgg
     2401 gtctaagatc caccaccaca ctcacaacat cgggtattac gtctcctgcc ttttgtatta
     2461 ttactgtatc tccaacacgc acatcgagcc tctgtatctc atcctcgtta tggagtgtcg
     2521 ctcgagatac ggttgagccc gctactctta caggccgaag gttggccacg ggggtgagaa
     2581 cgcctgtacg accaatctgc aaagttatat cctcaacaac agtagttacc tgttcggcct
     2641 caaacttaag cgcaataccc cagcgtggtg ccttgccggt gtaaccaagt atctcttgag
     2701 ctcttctgtc attcagcttc accaccactc catctatcaa gtaatcctcc ttatccttct
     2761 tctttgacca caacttccaa tactcaacca cctcatcaat gttatttgcg accttgtggt
     2821 gtttattgac cttaaaacca agtacttcta acctatctaa ctcctcttct tgagtcttgg
     2881 gcaagttatc agccttagac acatcataga taaatgtaga taaacccctt tctgctacaa
     2941 gcttggagtc tagttgcctt actgtgccgg caacaatatt tctagcgtta gcaaactctt
     3001 tttctccccg ccttctttgt tcattgttta tcttttcaaa ctgactcttc ggcatataca
     3061 cctcgccctc tacaatgaca gagacatcct cacttagtct aagtggcaca gactctatgg
     3121 ttcgcacatt gcttgttata tcctctccca ccttaccatc acctcgagtg gcaccactta
     3181 taagaatccc gttctcatac tcaagtacca ggtgcgatcc atctatctta agttcacatg
     3241 tgtatacagg ttctatatcc tcaccggtct cagtcttgta aaatctcttt actctctcgt
     3301 taaattccag aatatcctca acagtaaagg cgtcgttaaa tgaccattga gataccttgt
     3361 gtgtgatttt cttaaagcca gcacttgggg ccccggccac tctttgagtt ggagaatccg
     3421 gtgtaacaag ctctgggtag tctctctcga tcttcgtaag ctcatcctta agagagtcta
     3481 aggcctcagg actgatgctc tcgagattaa gtacgtgaaa atcgtatcga taacgatcaa
     3541 tagtcttgcg aagtttatct actcgctcct taacctcttt tggaactggc ttcatattac
     3601 ttgtagtgag cccgtcgaac taatacgaca caccaatggc tctttggact cgacgaggat
     3661 tggtagggtc acaagtatta tacccgtaag attcaattgt tgtactcttg atattaaggc
     3721 ttggttgagc tcttctcctg gttactctca cctctgtgca atatagatca ggtaggaagg
     3781 ttagtgtgaa gtttgactgt ccactactac cacccacagt aaaactctga ccgttacagc
     3841 taataacagc tgtagttgat gtggcaaagg cactgagtcc tcctggattc tttaggtccc
     3901 agtatagagc gcattctata cctgtatctg cagcataaaa tgcttcttga gagtgcctat
     3961 tcgcagtggt gaggaaagct tccttaaccg caacagagat gatacccgaa gatacaagca
     4021 aaagagttgc tgagatgaca atcgctatga atagtgtgaa gccgcggttt ttcattgcca
     4081 gttaaataaa tgctctgttg cagtaaagct cctatccaca agcaccccct tagaccaaaa
     4141 aacttttgct tggatcacca cctcattacc attacttcct ggaaccgagg tcacggtcac
     4201 ctctcttcta aactttgtgg ttgtaccatt gtcataacta tagaagccat ttgagttcaa
     4261 aagtaggttc gggcaagttg cagggcactg aatgagaggg gcactagagc ttaaagcatc
     4321 tacataacat gtgttaccga cagcacacgg gtcacccgag ttcgcaatac cagcaagcca
     4381 cccttgagtg ttgcctcgta taccattttg atctcggata ttccttatct gctctatcgc
     4441 ctcttgcgca agatagaagg cagttacctg atccttagaa tatagagatg acatcatacc
     4501 gttgtgagca gcagcgaagg cacccgtcac cgccaaaact aagatagtga tagcaataag
     4561 agtctcaact agagtgaatc ctttcttatt cgtttgtaaa ttttgtnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnntcga gcattggtaa caaaggacta caaatatagt
     4741 ttgtaccgaa gcgcatctca cgggccatgc tctcaatagc gagattcaag ttatccataa
     4801 cagtcttgat cgactcggac ttcctattaa catcaaagac tgcaagtatg gaacccatag
     4861 atatagtagt aataacagca aatatcgaca cagctgtcat tatctcgata agagtaaatc
     4921 cttttttcat catagtgcgt ccatacatga tgttcctcta actgaaacct gtcctgtggt
     4981 ataaacaaca atcgagttct ttctaccatc tgttgcagac acttctatac aagctgtatc
     5041 tgtgagtaca ccagtattat tgaaggttat gcgagcatcc acagccggtc ttacaaaagt
     5101 gatgtccaac tgctcatttg tacaattctc tgtgccgctc tctaaactac aaagtgctgt
     5161 aatagtatgc ccttgcccca agaccacctt atccaaacac tcagatgctg gggtacctcc
     5221 tataggatta caattcatgt caccaagata caggccattt ggagcaacaa gggcagatgg
     5281 cttcgtgtcg aggaaaaaaa gataagaccc attgtcacca gcaggatatg acggaactat
     5341 actaaagtgc acaccatacc ctgcattaaa acctgttgtt gtgtaggcct gtgagcttga
     5401 gggagaaagt tgtttaaccg aaaccccgta gatctgtgcc tgtcttagtg agaggctcaa
     5461 gttgtttaca atattcttca gcgctgctcc tgctccaaac tgcttctggt tcgatgcaat
     5521 tacggtacta ataataatca taatagctat agagactaaa agctccataa cagtgagtcc
     5581 cctactaccc ttaattgaag agtgtttgaa catgaaatag atcaaaacca aaaaacacag
     5641 agacccaggc gccgataata aggaacggag caaagggtat ctcgctcttc attgtaatgc
     5701 cttttcgaga aaagagcatt atgggtaatg taactgctgt gcctatccaa aatgccaaag
     5761 cggtagccga atatccctgt gctgccccta agaggagtcc gattgataaa acaagcttag
     5821 catcaccgaa accaagtgct ctacctttag atactagcca gataagacca aaggcagaaa
     5881 agagaattgg tccagcaagc caatcattta atgaataagg aataatgaat attgggacaa
     5941 gcagactaag cccaatcgat aaataaacta aaaaatctgg aatgattttg tgtcgtaggt
     6001 cataaataag gatgacagta tagacagaga aaacaatagc cagcaacaag gagtaagacg
     6061 tgagaccaaa gactgagtag aaagaggcaa ataccattcc tgtccaaagc tctatcaatg
     6121 gatactggag agaaatatgg gtcttgcact tactgcatct accccgaagt agaatataac
     6181 taacgaccgg tataagctcg tgccaggtaa gagtcttgcc acatgtagga caaaaagacc
     6241 tgccgcctag agtaagtcca ctcctaaaac gcagaccaac cacatttaag aagctaccga
     6301 ttattacccc caggataaaa atgaagacaa tcaaactatc aatcagttag ttacaactaa
     6361 catcattact tgctagagcg ctggtaatac ccatagacct accagcactg tctacacacc
     6421 aatgtgtaat agcaccacca acaccctctg ctactggcaa agatgctgaa acagcatatg
     6481 cgctgtcagt tgcctgacaa ctaagaagaa cattacctgg ccaaacttga gaagtacctg
     6541 taatagtaga tactggagat gatgcatcaa agaacattgt ccctgtgcat gctggttgtt
     6601 ctgtgcccat gttagatgct atataagcac tattgttact atcgtagaat agctcagctg
     6661 atgctctaat actagaaagc tgagctttta ccttagagtt atttgccttt gttctggcgc
     6721 tgtttagcga agcgagtaca acagaactca aaataccaat aatagcgatc acgactagaa
     6781 gctctataag agtgaagcct tgttgatttg ttttattcat actcgcttta gtatacatca
     6841 agtaataagt ttatagacag gtcgctgcgg ttacatgacc ctgcacagca gttctaccca
     6901 ttgatctacc accactgtct acacaccaaa gagtataagt attggcacca acaacttccg
     6961 caatgggtaa gctcgcagaa acagcatatg cctgcgctgt agaatagcac ctcatgcttg
     7021 tgtttgttgg ccaagaacca gcagttccag ttaaagcgtt cataccagag ttaacatcga
     7081 caaacattcc acctccgttt actggggcgc cagcagtaca tgcaggagca gaaaccatac
     7141 cagttactgc aggtgcatat gaactattat tagtatcata atacagctct gctgcggctc
     7201 taacagtagc caactgggcc ttaactttag aattgtttgc tttgttacga gcactgttaa
     7261 gtgatgccaa tacgacggaa ctaagcacac caataatagc gatcacgact agaagctcta
     7321 taagagtgaa gcctttttta ctggtattta gcatgacgta gatgtaattg gtccaaccct
     7381 acccatcgat gttcctgtcg aatctacaca ccactcattg ttaccattat cgtctacgta
     7441 tagaggtgcg gacacagcgt aagcgtctaa tgttgaatag caggttatgg ttggattatt
     7501 tggataattt gcaggatctg tgaacgatgc catattgctt cctgtatcaa caaacatact
     7561 gtccgctgtt gtgcagtcac cagtaacatc accagctatt ccattgtaag tactgccgtt
     7621 gttgtcatag taaatttcag ctgaagatct agcatttgct agttgagcct tgatcttggc
     7681 attctgactc ttaccacgag cagcatttag agatgcgaga accaccgaac taagtattcc
     7741 aataatcgca atcaccacaa gaagctcaat aagagtaaat ccttttgttg tccttttaag
     7801 catgaaatta aaaaattatt acctagtaat tataccccta atttcaagtc tcaatctagt
     7861 attaatccac acatacaaaa aaactcggtt acccgagttt ttttgtatta tctttatagt
     7921 cgagagacta gcaagtaatg tctcctgaag cctgtgcagc tgatgtagat gctgatttac
     7981 cagagctgtc aacacaccag aaggtgcctg ctgtagagag gttagcggac acagcgtatc
     8041 ctacgggtga agttgcagta acttggcaac taagcaaggt acctgatggc catgctgatg
     8101 cattgttggt caagttagcc atacccgaag ttgtatcctg gaacatagta ccagagccag
     8161 cacaaactga tgcaggtgct gccattgcta caaccgctcc gtaagaactg ttgttgttgt
     8221 cgtaataaag ctctgcagaa gctctcaagg ttgcaagttg tgccttgacc tttgagtcat
     8281 ttgcctttgt tctggcactg ttaagtgatg cgagcacgac tgaactcaag ataccgatga
     8341 tggcaataac caccaagagt tcgatgaggg taaagccttt tgttgtcctt ttaagcatgt
     8401 aaattataaa tttttaatac ccacttatta tacaccttag cctaaacgcc tgcggtgtta
     8461 gcaatattgt agatggggat aagcactgat gcaagaagcg tacccacacc caatccaagc
     8521 gctacgatca tcactggctc tataagagac accaaggtgt caactgcgtt aataacctct
     8581 ctttggtaga acttggccat ggtcttcaaa atttcaccca gttctcctga ctcctcaccc
     8641 accttgatca cttgagtgag gatcgagggc acctcctcgt attgcatcaa ggactgggag
     8701 agcgaactac cactcttcac cgctacacca ctgtctgaga gaatagtttt gtagatatca
     8761 ttacctacta catttgccgt aacctctatt gcacgaacca ttggtatccc tgagagaatc
     8821 atggttgcga ggttgtccga gatgacagag agatagagct tttgatacaa atcccccaca
     8881 taaggcacct cgagcttaaa gcgaccgagc gctcttttgc catcttgggt atttagatag
     8941 cgtataagca caaaagccac gataaccaag aacacgagta gaatgacacc atagtcgata
     9001 ataaagtcag agatagcgaa gacaattgca gtataaacag gcaccggctg accagaatca
     9061 agaagaataa ctgaaatctt tgggataatg agggtgaaca tgagcaccat caccgctacg
     9121 aacactccaa taacaaagat agggtatatc aaagcgttct ttgccttcga gactacctcg
     9181 taattcctgt ccaggtggtc agcaagatag tcgaatgtct cattcaattt ccctgtctcc
     9241 tctccggcct tgaccatgtt tacatagaaa tcagagaaga tgtctgggtg cttggaaagt
     9301 gctttcgata tggaactacc cgcctgaagg tcgtctgcaa tctcgaccat gctcttccta
     9361 agatgaatgt tctctatctc tcctgaaagt agagaaaaga tcttcaaagc tgaaactgag
     9421 gcaccgaaga gagtggcgat ctgcctactt aggatcacga tgtccttatt cttcacctta
     9481 gcccctatag taagcttcga gtaccatggc tccttatcag cctcatctat tgaggcaata
     9541 attagtcctc tcttttgcaa actgtctatt gcaacctcaa gcgaaacagc atcaatagat
     9601 ccctcctttt gaactccagt ttgttccagt gctttgtagt tatatagcat tgtcgtattt
     9661 aaatcatcct ctcaagcgac cttggattag tcgagtattg gaaggcattc tctggtgtga
     9721 tctcgcctaa acgcaccaac tcagaaagcg agcggttgaa gtcgatcatc ccatcctcgc
     9781 ttgaggtctc gatgatagag ttgagctcgt gagtacgctt ctctcttata agattcgaca
     9841 ccgctgtgtt attgataagt aactcatatg cagggataag tcctcccttg atacgtggga
     9901 taagtctctg ggagaagata ccaagaagcg aactagaaag ttggtatctt atctggtcct
     9961 gactctcagc agggaaagag tctatgatac ggtctacagt ctgtgcagca gtgttggtgt
    10021 ggagtgttga gagcacaaga tgacccgtct ctgcagcagt gacggcggtt gcaatagtgt
    10081 gaggttcgcg catctctccc accataatga catcggcatc ttgtctaaat attccccaaa
    10141 gcgctgtgtg aaagtctggt gtatcagtct ttacctctct ttggtcgatc attgacttct
    10201 taggctcaaa gaggaattca atcggatcct caatggtcac tatgtgttcg agtctatcgt
    10261 tgttgatgag ctcaaccata cttgcaagtg tggtcgactt accttgacca gcaggaccca
    10321 ccacgaggaa gaatccttgt tgtttagaag tgaagtgact taaaattttg ggcagattca
    10381 attcttcaaa ggtcctgatc ctcgtcggta taagacgaag agcgatagca cgcttgccga
    10441 cagtctcaaa tgcattacct cggaaacgtg ccccactctt atgagagaag gcgaagttgg
    10501 cttcctgctt catctcaaac tccttcatat tctctggggt aagaaagatg ttaaagaaac
    10561 tcttaacatc atcagcagtc atggcaggat atttctctag cggtatgagg aagctcgaca
    10621 ctcttataat aggcttccta ccctcagcaa tgtgtagatc agaggctcct tccttgttaa
    10681 caatatcgac caactcgtcg agtagagagt gagatgctgc catgggttaa ttttggccgt
    10741 cagctatctt gataatctcc tccgccacct cagaaggtat cgaggttgcc ttaacgatat
    10801 aacctgcaac ccctaaagac cttgcttcct ctatcctggc tgaggtgttc tcgttcgtaa
    10861 gcatgataaa gatcgagctc tcagcaacct tctcttcacg aatttttttc aaaagttcta
    10921 gaccatccat accaggcata atgacatcga gtactacgat gtcatacttt gccccctctc
    10981 ttagtttgtt aagagcttct tcgggtacag cgcacatctc ggctttagca ccaagcttat
    11041 cgaacttctt ctgatacatt ccaaggagaa atttatcatc atcaaccaag agtactgtgt
    11101 atggcttttt catatcttta ttatagcgta tttacttctt cgaatggaat gattcggttg
    11161 aaagccttga taatggcatc ctccttcatg gtgagcatcc ccttcgacct cgccatcttg
    11221 aagagctcta tctcggttgg attcttcaaa agcacctgct cgatatcctt atccatcgag
    11281 agaacctcca taaccgcaac acgaccagag acaccagtaa cactctctgg ggttggtgct
    11341 gcctcgtaga actccttgcc cgttggaatc tttttcctaa actcagctgg taggtcagca
    11401 aactgtttct ccatcatgag cttcatactc tcagacactg gtaccggctt accactaccc
    11461 tcaaccaatt tagataccaa tctctgagcc atggcgagga cgagagtagg tgcaataagg
    11521 taaggatcca cacccatgtc aatcaatctt gggatcacac cgatagcatt gttggtatgg
    11581 atggtcgaaa gcaccaagtg tcctgtaagc gccgcttgga tagcgagctg ggctgtctcc
    11641 ttgtctcgaa tctctcccac catgattaca tctgggtctt ggcgaagtgt agtacggaga
    11701 cctgtggcaa aggtgtaatc aatctctggc tggacctgtg actgactcac cccctccatg
    11761 ttgtactcaa ctgggtcttc gagcgagagc acgttctcat ggtccttatc gagctcattt
    11821 agcatggcat agagagtggt ggacttaccg cttccagtag gaccagagat tagaatgagc
    11881 ccgtaaggac gatcaatagc gtctttaata agcttcatat tctcatctga taatccaaga
    11941 tcctttaggg tcttaacccc cttagactgg tcaagtattc tcatcaccac cttctcgccg
    12001 aaagttgttg gaaatgtcga tacacggaag tctattcgcc tatcatcaac cttagcgcta
    12061 aagcgtccgt cttgaggctt cctcttctcg tcgaggcgca tgttggagag gatctttata
    12121 cgcgcaacca cggccgagtg gaccttgata ggcagagtaa gtgaggtatt gagcacgcca
    12181 tcaacacgga agcgaactct aactgtctcg tggagtggct cgatatgcac gtcagaagct
    12241 gctccttcgg cagcataaga gaggatggta gccacaatct tagtcaccgg tgcatcctca
    12301 acgatctttg cctctctatc ttgctgagcc tccttctcct tatcggtctt ctccaggatg
    12361 acattctccc tctgagtaag ttcactatcg agctcagaaa gggcctgagt cacctcactc
    12421 gaaagtcctt tgtattgtgc aaatattcgc tcaaaatcct cctgacttat taaaaatagt
    12481 ttataaggca tccccgcctt tgatgaaata aagtttagag catctctcgc ctctatatcg
    12541 tcagggtcaa ccacaccaat ctcaagtacg ccgtctttga taccaatagg cacaaccttg
    12601 taatgctctg cactctcctc agatatatac tcgagcatct tcgagggcac tgaaacattt
    12661 ttcaaacttt ttgttgggat ctcgagctct gcacccttag cttcaaggat tttttgtggg
    12721 ggcaccccac gacctactag cacctgctct aaggaaagac cagtgctctt ggcctcttct
    12781 tcgatcgagg cgacatcggt cgccttaatc accttcttgt cctttagtac atcaagtatt
    12841 gtcattaaaa actggcttaa tcgaacccaa cgggactgaa tgggttactc ctgccccttg
    12901 gctgctcaag tattggtact gagaaatcta ttagtttgtt atataaagat gaggtaaata
    12961 aaccttgctt caaattcaca gaagttattt tgctctgcag ctcaatcacc tcggccccca
    13021 ctgcctgagc agtcaaatcg tctggggtca ccacaatctg agaatcaggc ttgaagtatg
    13081 cactgtagaa atagaatcca catgccaaaa gcactaggat gaggattata tttttatttt
    13141 tgtggaagaa atccatggta ttaattattc ggttttgagc caataagtct gcaccgccaa
    13201 actatattcg tacaaaccat catcattcga ctcaaaggaa acgctcttta tatctgtgat
    13261 cctaagactc tgctctatgt caccaataaa gtctctaaag tcttcgtaag gtgcagtgaa
    13321 ggaaaagatc acggtaaccg gtttataaga cccgcctgat gagtcttgta caatagcagc
    13381 tccggactga tccacctggt ccacagtact aatcttcttt attgatatgc cgtacttggc
    13441 agcaatggaa tcgaagttca ccgccaaatt aacagtgtcc acattgtcgg gtagtacctt
    13501 ctccaactta gcaatctcgt tttgaggcat attttgatat ttagcctcta ggtcgttcct
    13561 cttcttgatc aagatatcca cctgatctaa agagttttta tactgagcag actgagttct
    13621 aagaatcata atcttgtcgt aataaggaaa acctacggta taaaccagtg ctacagagat
    13681 caaaagtagg attaatgcag tagagttctt catattattt attgagtggt tccattagtc
    13741 gtgactggct cttccactga tactcccgct tctactggcg cctccactac cgcttctggc
    13801 tcgtctcctt tattgaaagc aatgatagac ggatcgagag tcgccttaaa agtgaagtct
    13861 acattacctg tattatcaag agacagatcc ccgaatacaa cgtctttcat aaaatcacta
    13921 gcattaaatc gttgagcttg gaaagctaga gtggagtacc cacgtgactt acctcgcatt
    13981 gtaagcttca aaaccccttc ctctgaagta tatttgaatt gagtaagctg aatggtctta
    14041 acagtctctg tttcaaggta ttcaaaaagt ggagagagga cgatatggtt attcaaaaga
    14101 ctttcagttg cctttagacg ttcatctagg tcactgatct tcttaattac ttctggttga
    14161 agagttgatt tagcatccaa aagcttcttc gcaagactgc caatgtcttt cttcaaataa
    14221 tactcataac caacagcact aagagtagca aggatagcca aggcaaaaac aaagacagct
    14281 aaggtaccga gaaaactcgt atcagactgg cgagaggtct tcgttgctgt accagtcgtt
    14341 gcgagtggac ccttaggtat gaatgatgat tggaatttcg gttccatctt tacaaattat
    14401 agcttaatta ttggtcaaaa ataaaaagtt atacacaatc ctagtcattc aacctcctca
    14461 aagctgcacc gatagccaca gcgaactcag gaccagtctc cttcaaaatg ttttcgataa
    14521 atgctggcgt cgctagtttg ccaaaggggt tacccatcac tgcttcggtc ttgaaggcgt
    14581 tcttggtgag ctcatttaac cccttcatcg ctgccccacc acccacaagc accaccttag
    14641 atacactttt gttgtatctc ttctcaaaat tgagcagagt tctgttggtc tcggcgaaaa
    14701 taaagtctag tacgagagaa ataatcttgg ccaaagttgg atcttggcca gacgcaccaa
    14761 tctctcgctt catgatctca gccttctcga tagaaaggcc aagtgactga gcaatggccg
    14821 aggtaatgtc ctgggcacca cggttcaccg tgtgcgaagc acgaataatc cccctctcga
    14881 taatgtagag tttggtcgaa gcggccccca tatccataac cataaccgga gtgagactct
    14941 cgtcgaccac actcctcatg gtggagaaga tctctatctc aaagaaacta ggctcgagac
    15001 cagccttctg gataatattt tgatatctgg caatagtatc gttatgaatt gctacaacca
    15061 ggacatctat tcgcttcaag gcattcttct tctcctcatc gtcttcgtct tcattagggt
    15121 ttgaaggctt ggggataacc gaccaatcta gcatcacttc gtttactggt acgggtatat
    15181 acttcctcgc ttcaatgggt accattacct cgagttgctt ctgacttacc tcaggcatct
    15241 ctatcacaga catcaaagac gaagcgaaag gtatagagat ccccgcttgt gtggtggtga
    15301 tgttgacctc cttctctcgc atgaggtcaa agagtgccga agccaccttc tcttgtggca
    15361 aattcactgc ctgaccaatg gaagcaccac cgtatggacc aagagcgagc tctccataag
    15421 tctcgagtac cgcctgtcca cccttcttct tgatctggac tactttgata gcagaagagc
    15481 cgatgtctat ccctacaact gagggagact ccttcttgcc aaagaacttc ccgagaaatg
    15541 ccatataatg cttattatac attatgttta gagctttaat ctctaactta ttcgattttc
    15601 tctatcctaa ggatactagt gtaatctctc ttgagagctt aaaacctgaa gaattgctca
    15661 ccaagctcac cacagcgaaa agtttaagca atgacacctt cgctatatgg aactatgccg
    15721 acgagaaggt gcgcaccatc atctgggaga taaaatacag aagaaatgaa gtacttttaa
    15781 agaatgtggg tcaagtcttg tatgacacag tgtcgagcga agtacttgaa agaatggtaa
    15841 ccgaaaactt tgttcgacca ctcctactac caataccaat atcaaagagg aggagactcg
    15901 agcgaggata caaccagact gaattgcttt gcaaagttat gaaaaaactc gatacagaaa
    15961 atttgtttga atactctccc aatgcactaa ggagagtcat ccataccgaa agtcaggctc
    16021 gcaccaaaaa taaaaaagag agaataacta atatgcagaa cactatcgaa gcaaatgagt
    16081 caaccaaaga tagaaacata atacttatag acgatgtaac gacaacgggc gccaccttgg
    16141 gtgacgcccg tcgagctctc cgcgagtcgg gtgcgaagag gattatttgc tttgcactag
    16201 cacattaacc acaatcgagt attcttagtt tgcttctatc gctctcacgg ttataacacc
    16261 atcataggca tagtccccca cctctggatt ctctgcgtcg gttgcgacaa aagtccccag
    16321 tactcttacc ttctctagat tactaccttt agcatcctcc attttagaag tatcgagcga
    16381 gtaaaactta ccgtcgtctg ccttaaggcc catgacacac tcaccttctg ctacaggctt
    16441 accactcttg tgtggagtac agtcaagtac tcctgtcgca tctatctcgc cagtacgagg
    16501 ttcgaaggaa acaagcgcca ggttcgcatc agtattcgtt actaccttag gcgcatcact
    16561 attcttaagt aggtagcctc cgccgataag ggcgatcgct acaactgcaa tagctaagtt
    16621 cctaggcatg ttatttattt atcaccatta gtctaatagc catcacaccg tcgtacttga
    16681 agactgttgc ttcctcactt gaagcatcta ccttggtgta tgtaccaact accttaacaa
    16741 ctgagtttgg ttcgatacca ctctctgcca tctctagctt tgagtattca agcgcgtaga
    16801 acttaccatc agaaccaacg agacccaaga cacattgctc gttagtaggc tgcgcaccgc
    16861 tcttaagtgg tgtacaacca agcttaccca caacggcaat ctcaccactc tttggttcaa
    16921 cattagttgt ctcggttacc tctccaacca caccaccctc agtctttggt gccttatctc
    16981 caccgaaaag tactagacct agagccacaa caacgatgag gacgactccg aggaccatct
    17041 tgttcttata ccaagcactt tgcatattgc tgtccatgtt tttgatcttt cttattaaat
    17101 ttggttacca atctactata taccattcag accatttcac aacaactgct tcgggggtca
    17161 aattagccct tatatgcctc ctcgagcttg gccagattga tcctcttcat ctctagcatc
    17221 gccttaagag ctctatcagc cccagctttg tctggcccgg taaccaattc ccccatacgc
    17281 ctgggtatca cctgccaaga gaaaccgtat ttgtcctgta cccaaccaca ctgctccgct
    17341 tcaggattag ccgagagctt gtctgcataa tagtccgtct cagcctggtc ctcacattcg
    17401 atagtgaagg agaccgcccc gtttggctcg aacttgtgct ctgcactact ctccatagcg
    17461 atgaatgggt gattctcgag tgagaagctc gagtacttca ccttgcctgt tacatcgctg
    17521 tccccctcct cgtacttaac gaccatatgt ttagaagagt ttttgaaaac tgaggtataa
    17581 aaatcaatcg cctcctcgac atgtccaaaa tacttaccaa caaacatcat tgagggtacg
    17641 atcttcacca cgccaggctc catattgatc tgccatgaga gcccgaactt atccttgaac
    17701 catccatatc tctcagccca tggatacttt tggaatggca tgagatcctc gccaccctca
    17761 ctaagttttt tatacattgc ctccgtctcc tcgactgttg gacaagtaat agtgaaggat
    17821 acagaagggt tgaacttgaa gtagtctcct ccgtctaggg ccataaactt ctctccagcg
    17881 agttcgaata cggctgttag taccttgcct cccatacctg ggataggacc aacttggaaa
    17941 tcggttgggt aacgcttgat agtagtgatc ttcgagttcg gaataataga agtgtaaaag
    18001 tttatgactt cctcgcaatt attgttgaac caaagtgatg gaattatttt ttgcatcaga
    18061 acattctact actacttcaa ataactatcg aaccagatgg cgtgacaggt gtagcctccg
    18121 acattcttct gcaagtagtc ttgatgatac tcctcagctt tgtagaactt cgtaagaggc
    18181 tctagactcg tgaccaccgg cctctgccat ctcttagagt cgtccactat cttgataaac
    18241 ttctctgctt cctccctctc ctcctcattc gcataaaata ttgcagatct gtatgaggta
    18301 ccaatgtcgt taccctgttg attcaaagtt gtggggttat gtatctggaa gaagaaatcc
    18361 aaaagcttct tgtatgtagt ctgtgtcgga tcgtactcta tctcgaccgc ctctgcgtgt
    18421 cctgggtggt tcgtataagt cggattatca ttctccccgc cggtataacc cacctcagta
    18481 ttaactactc caggctgact tcgtatcaac tcttcgagtc cccagaagca cccgcctgca
    18541 aaaaccgctt tcttcgtcat cggtttaacc aagtgatagt gagctgtaac actgtggcga
    18601 aggagaccca aagtaggta
//
LOCUS       DAORIT010000010        18208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_501_length_18208_cov_46.571035, whole genome shotgun sequence.
ACCESSION   DAORIT010000010 DAORIT010000000
VERSION     DAORIT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 18208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18208
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_501_length_18208_cov_46.571035"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLD99_01460"
     CDS             <1..664
                     /locus_tag="PLD99_01460"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRH25605.1"
                     /translation="RTAVSKTAGEGSIPSSPAMTDLFNIPYLLSTYGYVGVFVIVFLE
                     SGIFFALPGDSLLFTAGILASGLGFKLTLLVPLIFAATFLGGIAGYFIGVYLERLHKY
                     RFFQRLIKREHLDKTHTFFEKHGLFAILLSRFVPIVRTFLPIIAGVARMNFGKFLVYS
                     FWSSLLWALSMTSLGYFVGMQFPEIRDYMHYAIILVVLVSVVPIALEWWREKRFLHQG
                     AK"
     gene            complement(579..1481)
                     /gene="miaA"
                     /locus_tag="PLD99_01465"
     CDS             complement(579..1481)
                     /gene="miaA"
                     /locus_tag="PLD99_01465"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRH25606.1"
                     /translation="MKPKVLVIVGPTASGKSDLGVALAKKLLGEIVSADSRQVYKGLN
                     HGTGKIIRSEMQGIPHHLLDIVSPKIQFTATQYRELALKALREIVARRKFPIIVGGTG
                     FYIDTVTCGVLYPEVPPNKKLRDKLSKLSVEKLFDMLKAKDPNRASLIDSKNKVRLVR
                     ALEIISTLGSVPKIKHVTPEYEFVFVGLNPKDLDERIERRLKKRLPKIITEVKKLRSE
                     GLTWKRLYELGLEYRYVSLHLRGVMNKKEMNMELLKEIRKYAKRQMTWFKRNKSITWL
                     PDGGNASLSTILKLLGQQKRVQPK"
     gene            1515..1817
                     /locus_tag="PLD99_01470"
     CDS             1515..1817
                     /locus_tag="PLD99_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HRH25607.1"
                     /translation="MKGFLDFIREQGVVGLAVGFILGGAVSKLVSAIVTDIVNPLLGL
                     ALGSVSGLKEAYFAIGSAKIMYGDLLAVAIDFVVIALVVYFGVKALKLDKLDKPKI"
     gene            complement(1847..2185)
                     /locus_tag="PLD99_01475"
     CDS             complement(1847..2185)
                     /locus_tag="PLD99_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25608.1"
                     /translation="MANCSFVIVHCIDFRTQKSGKKLRRSLGISEKDCDVISVPGGAG
                     NIEQLKHCLELSHRLHHTSTAILTVHEDCGAGAKKEDLKKAASIAIQIGLKPRCFFIK
                     LDRKWEEIMV"
     gene            2389..2976
                     /locus_tag="PLD99_01480"
     CDS             2389..2976
                     /locus_tag="PLD99_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRH25609.1"
                     /translation="MYIYLALAGAGLIFFVSSLFHGDGDAHDFHHGGDGHDHDSSDGN
                     GDHGTQFRDFLSLRSIALICLGLGLTGAVMGALGIFGFIVPIIGTAFGVLLAYMSIKA
                     IRFMLKQEATTTSSLDDFNGMNGVVSIEIPADGIGQVCVTSFKGEGRYITARSTDGNS
                     IAADQPIEVVEVVNGELKVRKVQSLPESSSEVLSK"
     gene            3015..4814
                     /locus_tag="PLD99_01485"
     CDS             3015..4814
                     /locus_tag="PLD99_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRH25610.1"
                     /translation="MQDTGGLGITTFVIPTAILLLAVVTVIVVRFLIKQYNRVSPNTV
                     AVVYGKRNGEKGYRIVTGGGFFLWPVIEEVKYLSLNIMSIEVAVVNAPDKNGVLLSVK
                     GVANVKIRSQTEHLPAAIERFLGKSEEEIKRIAKDNLEGNLRALTGTLTVEELIQDRT
                     KFQAQVLQQVGADMAKLGLDIDLLNIQNITDDKGYIDSLGRKQTAGIHRDAEIGEAEA
                     RRETAIKTAEARQAGEIATSLADQKVSDAHKQRDMAVAENKAQVEAAQARISIVSQAA
                     AAAEQKKLNIATVEAEQAQVEAEIGLQESVRKRTEAKLNATTIVEAEKGKEAKIIAAE
                     AARRARVIEAEATKEARIIEANADQEASVLEGEASRIKSEKEGLGAQARMTAEAAGRV
                     KVAEATRAEKEAEAAGRKALLLAEAEGEEARLMATARGTQATLMAEADGRKALLLAEA
                     EGAEKKAAAFAKLDEASKAMLILEHLPAIIGALGDAASKVIVPAAEAIGEGLGNVKEI
                     RLVSMGGQNGSVDGNVLSQFMKAPSEALFGLFQRINAAGLGPMVKSMLSKYGIDVSDL
                     FPEPAKLAGGSEVPATAAPLSTSKEGSAKVGSK"
     gene            complement(4882..4962)
                     /locus_tag="PLD99_01490"
     tRNA            complement(4882..4962)
                     /locus_tag="PLD99_01490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4927..4929),aa:Leu,seq:caa)
     gene            5008..5976
                     /locus_tag="PLD99_01495"
     CDS             5008..5976
                     /locus_tag="PLD99_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRH25611.1"
                     /translation="MSQIYNDAIFWIEVDKIHPNPYQPRREFDELALKSLADSIRQYG
                     VLQALVVTRKEVEKPDGGLATEYELIAGERRLRASKLAGLTQVPAQVRNGEETDLMKL
                     ELAIIENIQREDLNAVDRAKAFDKLAKEFGFKHAEIGKKVGKSREYVSNTLRILMLPD
                     EMLNALAEGKITEGHTRPLMMLVDKPEEQMTLFKEIMFKKINVRDAEGIARRIAFEKA
                     RKLMDPDLVDLEDKFKETLGTRVRIEKRDDGGKLTIDFFSKDDLISLLEKLSNEVHVL
                     PDMIASTPTSEVPVPTEEEKEVMSEGSKEAMEASDEDLYSIKNFSV"
     gene            5985..6626
                     /locus_tag="PLD99_01500"
     CDS             5985..6626
                     /locus_tag="PLD99_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25612.1"
                     /translation="MKKQYIQIGALVVVLVGIVLFVPFNKPEIKDLEDIKTGELKTST
                     KSSTSGSTTVNTPTNTYNPSNKEFPFSFTEKNLVLGTPFGSFKYSGFDYANYHKFSGS
                     ATVSGQVTAVKTDGEDNLGITVDKADMYKLPQYYFASIQGENGTYNYVRAEKPYVVCF
                     GYYYPSQYDYNELLGKLALKAGERVTVKIDNYKIIASKEHVGACASLLEIVGN"
     gene            complement(7048..8475)
                     /locus_tag="PLD99_01505"
     CDS             complement(7048..8475)
                     /locus_tag="PLD99_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25613.1"
                     /translation="MHVALDNMATALGIRPNSLRIKLGIVTRLQGQVRAAKTFLDMVA
                     LEPEFRKKMHSSCGPKFEPEELHEYKARRGEFFSDYLGQFSTIEQFKSFGKIAAGPSL
                     SFYNDEWSTASSKLYSLIKEMLESANDLTALIEVHQVVEYFHKYFKIYDQGFAQDLKY
                     KMIRYFIEALRSTKSLKDRLKIIKTMPISYYDLFGFEHHRQEKSSAQWKFVWEEIESL
                     EDAFLVEQTKTGDFISSDGLKVRMTTLLDKHMGRMRGFRQRLAFANSLAKRDNNGPDY
                     LHSRSLELVAEKASTLMQVKEIIKIWRSRPKNSVGSGPHISWPELPQIMLDKWDDLKL
                     YVAEESVDANTLAELLESSPFGTKTRQVALDKLNNHFKQMIDSAQTEEELDQVEKDAK
                     SLSGSISTWGYERRDAIRAKKNQRRAEAVLSKTTMGEHPDPKKALIGLYHHYKESEPE
                     QANKILRMLVIYFPAEAVEEVPVAQ"
     gene            complement(8559..10712)
                     /gene="topA"
                     /locus_tag="PLD99_01510"
     CDS             complement(8559..10712)
                     /gene="topA"
                     /locus_tag="PLD99_01510"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRH25614.1"
                     /translation="MRLVIVESPSKAKTIEKYLGKDFRVVASVGHVRDLPKNNAKAID
                     IPHGFVPHYEVSKGKDKVIAEIKAIAKKASEVILATDPDREGEAIAWHIADAIGLKKP
                     KRIVFHEITKEAVEEAIEHPRLIDENLKEAQEARRVLDRLVGYDLSGIIWKKVRYGLS
                     AGRVQSPALRILVEREREIRAFNPETFWKIFAELNKLNFECSIEPRDKKEVESILKNG
                     EKGSWSVIDIKETEVKRSPRAPFITSTLQQAASSRLGFAPSRTMGIAQKLYESGYITY
                     MRTDSTNLGAQARADIKSTIISKFGESYYESHVFSKKSKNAQEAHEAIRPTHMNVENA
                     GFTDEQKKLYKLIWSRTLATQMKDAVVMRTKVVANIKDKSIPDFWLNGSRILFDGWTK
                     ADKAAQGEEVILPKMSVGDKLDLDKIFSEEKQTEPPSRYSEAGLIKELEKRGIGRPST
                     YASIIKTIVDRGYVEKDQKALKPTDTGEVVSGFLEDNFGEYISDTFTADMEDKLDDIA
                     LGKRTYVKTLTDFYTPFNKDVKSKEKLAKATDLGTADKKYKCPTCGSSMIIKLGRGGR
                     FLSCSRFPDCTGALTINGEEIKADTPIGVNPESGLSIFVKTGRFGPYVQEGEDDKKKK
                     IKARKASIPKTKNLSEISIEDALVYLSLPRVLGTHPDTGEKITASIGRFGPYIVHEKD
                     FRSLKTDDVYKITLERALEILKEPKKTRGFKKKEK"
     gene            complement(10777..11631)
                     /gene="dprA"
                     /locus_tag="PLD99_01515"
     CDS             complement(10777..11631)
                     /gene="dprA"
                     /locus_tag="PLD99_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRH25615.1"
                     /translation="MIRDLLEEEWPELLKEINDPPKSLRLWGEPPIEGNKLLAIVGSR
                     KFSLYGQEVTSSLIQGLAGYPITIVSGLALGIDSIAHRSAMRAGLQTIGVPGSGLDKK
                     VLHPHSHVSLAEEIVREGGGLISEYNDTMPSGAWAFPRRNRIMAGMCHATIVIEAEKK
                     SGTLITSRLATEYNREVGAVPGPISSSTSDGPHMLIRLGAALIRDSHDILELLGLRSE
                     GHDTLLNIDDLSDEEKAIIELLDKPKERDVLVRESGLETHQVTSTMSLLEIKGLIVEE
                     LGLVRKTF"
     gene            11649..12929
                     /gene="serS"
                     /locus_tag="PLD99_01520"
     CDS             11649..12929
                     /gene="serS"
                     /locus_tag="PLD99_01520"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRH25616.1"
                     /translation="MLDIKFIRENKDLVAMAAKKKHIDFDVEKLLDVDEKRRSLLQKV
                     EEKRGEQNMASKEIMSASGPERANILEKMKFVKEELQKGEDELKELMKDWQGLMLQVP
                     NVPDISVPDGVSDEQNLEVKKWGDLPRFDFEPKSHVELMENLGMLDLERGTKVAGFRG
                     YFLKGDGARLQFALWRFVQDYFAKKRDYVPMIVPSLIKREVFFGTGYLPQGEEDLYKT
                     QDGEYLSGTAEVATMGYYMDEILDKKDLPIKHLSFSDAFRREAGSHGKDTRGILRVHE
                     FYKFEQVILCEANHEESVRFHEEIQRNVEEITEALGLPYRVVVNCGGDLGLGQVKKYD
                     TEVWLPSEKKYRETGSASYFHDFQTRRLGIRYKDTDGSLKFAHSLNNTALSGRPLIML
                     VENFQEADGSIKIPEVLLPYFGKDKISRDETSRE"
     gene            12910..13734
                     /locus_tag="PLD99_01525"
     CDS             12910..13734
                     /locus_tag="PLD99_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRH25617.1"
                     /translation="MKLVGSKGAVRSAEFHKKKEREKKFYQLLFTLAFLLIIVGPIFL
                     LRSEKLKVSQVSVSGNMVTQSSEVESLVKERLSGYYLGLIPKSSTLFYPKDEIVDTLD
                     KQIPRLASASVSLNGFRGVSVEVVEREPAALYCAHVVDKESCYFLDNTGYIYSEAPDF
                     TDGVYLVYRRDPSINDPVDKYILEENEFARVNKFINSLGKLEMKSVSILNKEGDYILT
                     LDNGPEIMWNSNQDLDKVLVDLESFLKGSKLTMSDIKNLLYLDLRFDNKIYYKGRE"
     assembly_gap    14193..14292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14293..15246
                     /locus_tag="PLD99_01530"
     CDS             <14293..15246
                     /locus_tag="PLD99_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRH25618.1"
                     /translation="MNFWKNLKRPIFVLAPMADVTDSAFRAIIAKYGKPDVTWTEFVS
                     ADGLALAPEEGRKKLLKDLEFTNAERPIVAQFFTSKPENMRKAAALAKELGFDGIDIN
                     MGCPDRSIEKQGAGAAMMKNPKLAREVLRAAIEGAAGLPVSVKTRVGYNKVELEDWLP
                     EILKEDPAVVTIHARTRKEMSKVPARWEFVKRAVEIRNELGSGTLILGNGDALSIEDA
                     KKKVDETGADGVMLGRAIFGNPWLFSEKTPTLEEKLIVCVEHTKLFEKKLGGLKNFAL
                     MKKHYKAYVNGFDGAGDLRGKLMEAGNAEEVEDIVKNFLYN"
     gene            15834..17165
                     /locus_tag="PLD99_01535"
     CDS             15834..17165
                     /locus_tag="PLD99_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25619.1"
                     /translation="MRTRWFPRLVTFLVAILMFSACGDNKQITKVIVNIWLPLGTRTD
                     TTGTCVRTTMIGTSDTSNVAGSNILLPRGEADSLSVSQRFNPSGCGTPYTFRWSTSNA
                     SVVSVTAFDSVSARITAISSGTAVITATAVEDSTIVLRVTVVVPGVSTPTPVSISVEP
                     VSATLYSGQTMTLGTGTYRVVVTMSDGTTRVNDLSTFSCSSANTSQVTVNSSCLISAL
                     APTTSGGVVVTYAALGVTGVTDTLVVSVNALPVISLNPVSLCFSTVVGTPQSQTVTVT
                     VTGASVSNVVLASNTTGVTVSGSGSTWTVTRTAGSVVANGSLVATLTTMSGVVTAIPV
                     QIISTVCPGTTSSIDFTPSGGPLPIGLDTLTATCVQGAEIPNVCEPYWTSSDPSIATV
                     RGNTAKPVGPMTFWVGLIAYLERLRPGTVEICVQWSLTQTSPRRCYTFTSP"
     gene            17223..>18208
                     /gene="dnaX"
                     /locus_tag="PLD99_01540"
     CDS             17223..>18208
                     /gene="dnaX"
                     /locus_tag="PLD99_01540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRH25620.1"
                     /translation="MSVLYRKYRPKSFDDVIGQEPIVNVLKNEIKLGTLAHAYLFAGS
                     RGTGKTSVARIFANEVGTSKSDIYEIDAASNNGVDEIRALNDAVHTLPFDSKYKVYIL
                     DEAHMLSKGAWNAFLKTLEEPPAHVIFILATTELNKVPETVQSRCQSFVFKRPSQKIL
                     REFSQKIAKDEGRVLADDSAELVALLGDGSFRDTHGILEKIFSSTKEKKIEREEVEMV
                     TGAPRGELVRSVLEALINEDLEQGMKAIAEARENNVEAKIFAKLILERLRYLFLLRLK
                     AGMEDYISSETSDDEFEFLKTLASKPAKHLTSEVLLRFIEASEQSGRSSIPEL"
BASE COUNT         4523 a         4203 c         4602 g         4780 t
ORIGIN      
        1 tagaacagcg gtctccaaaa ccgctggtga gggttcgatt ccttcatcgc ccgccatgac
       61 agatcttttc aatattcctt atctgctctc cacttatggg tatgttgggg tatttgtaat
      121 agttttcctt gagtcgggca tcttctttgc gctaccaggc gacagcctac tattcactgc
      181 gggtatcttg gcctctggtc ttggatttaa gcttacatta cttgtaccac ttatctttgc
      241 tgcaacattt cttggtggca tagcaggtta cttcattgga gtgtatctag agcgcttaca
      301 caaatataga tttttccaga ggctcatcaa acgcgagcac ttagacaaga cgcatacctt
      361 ctttgagaaa catggacttt tcgccattct tctctcgcgt tttgttccca tagtacgaac
      421 cttcttaccc attatcgcgg gcgtggctag gatgaacttt ggcaagttct tagtctacag
      481 tttttggagt tctctacttt gggccctgtc gatgacttcc ctaggttact tcgtgggcat
      541 gcagttccca gaaattaggg actatatgca ttacgcaatc attttggttg tactcgtttc
      601 tgttgtccca atagctttag aatggtggag agagaagcgt ttcctccatc agggagccaa
      661 gtaatagatt tgttcctttt gaaccacgtc atctgacgct tggcgtactt cctgatctcc
      721 ttgagtaatt ccatattcat ttctttctta ttcatcactc ctctcaaatg taatgatacg
      781 tatctatact cgagaccaag ctcgtataga cgcttccagg taagtccttc ggaccttaat
      841 ttttttacct ctgtaattat ttttggtagc cttttcttta atcttctttc tatcctctca
      901 tccaaatcct tcgggttaag tcccacaaat acgaactcgt attccggtgt aacatgtttg
      961 atctttggta cggatcctag agtacttata atttccagtg ccctcactag acgcactttg
     1021 tttttagagt ctatgagaga tgcgcggttc gggtctttgg ccttaagcat gtcaaataac
     1081 ttctcgactg agagcttaga gagtttatct ctcaactttt tgttcggcgg gacctctgga
     1141 tagagtacac cgcaggttac tgtgtcgatg tagaagcccg tgccaccgac gatgataggg
     1201 aatttccttc ttgctacgat ttctcttaga gctttcaaag caagctctct gtattgtgtg
     1261 gcggtgaact gtatcttggg actcactata tcgagtaggt ggtgcgggat gccttgcatt
     1321 tcacttctaa taatcttacc cgtgccatgg ttgagtcctt tgtatacctg tcttgagtcc
     1381 gcagaaacaa tctcaccaag aagtttcttg gcgagcgcga ctcctaggtc gctcttgcca
     1441 gaggctgttg gaccgactat tactaagact ttaggcttca tatataatta tcttatacat
     1501 taatttatat ttatatgaaa ggatttttag attttattcg tgagcaagga gtggttggtc
     1561 ttgcggtcgg ctttattctt ggtggtgcag tatcgaagtt agtttctgca attgtaactg
     1621 acatcgtgaa cccgttactg ggtctcgcac ttggttctgt gtcgggtctc aaagaagcgt
     1681 atttcgctat aggtagtgca aagataatgt atggtgatct tcttgcagta gccatcgact
     1741 tcgtcgttat tgcgcttgta gtctactttg gtgttaaagc acttaaactc gacaagctcg
     1801 ataaaccaaa aatttaaaaa cacacgcagt taagcgtgtg tattattcag accatgatct
     1861 cttcccactt cctgtcgagc ttaatgaaga agcatctcgg tttgagcccg atctgtattg
     1921 cgatcgaagc cgccttcttc agatcctcct tcttggcgcc agcaccgcag tcctcgtgga
     1981 cagtgaggat cgcagttgaa gtgtggtgaa gccggtgcga tagctccaag cagtgcttca
     2041 gctgttcgat atttcctgcg ccaccgggca ctgagatgac gtcacagtcc ttctcggaaa
     2101 tgcccagtga tcgcctgagc ttcttccccg atttctgggt cctgaagtcg atgcagtgga
     2161 cgatcacgaa cgagcagttt gccattggtg cctcctaagg gtgaatatac gacccactaa
     2221 tccaaataga taattaacat taaaaatgtg ataagtcaat catacttagg cccactacct
     2281 taagtatagt tgactttttt taaattatat gatagtgtcc cgaacggaaa acagtcgatc
     2341 tttgactgaa aatcgtaaga aaaaattccc aaacccagag gttcgacgat gtatatctac
     2401 ctagcacttg ctggagcagg gctcatcttc tttgtctcat cgctatttca tggcgatggg
     2461 gatgcccatg attttcatca tggtggagat ggacacgacc acgactcttc agatggaaac
     2521 ggtgatcacg ggactcagtt ccgagatttc ctatctcttc ggagtatcgc tcttatctgt
     2581 ctaggcctag gcctgacagg ggcagtgatg ggcgcgttag gaatcttcgg gttcatcgtg
     2641 ccaatcatcg gcaccgcgtt tggtgtacta ctcgcgtaca tgagcatcaa agcaattcgc
     2701 tttatgctca aacaggaggc tactaccacg tcgagtctcg atgatttcaa cggcatgaat
     2761 ggggtggtct cgatcgagat cccagctgat ggcattggac aagtctgtgt caccagtttc
     2821 aagggagaag gtcgttacat cacagcccgc tcgactgatg gaaactcgat agctgcagat
     2881 caaccgatcg aggttgtcga ggtggtaaac ggagaactca aggttcgcaa ggttcagtcc
     2941 ttgcctgagt cttcgtcaga agtgttgtcg aagtaacttc cgcaggtcgc agttaaccca
     3001 tcaacgaggt aaagatgcaa gacacaggcg gtctcggcat aaccaccttc gtgatcccga
     3061 cagcaatcct gctgctcgcg gtcgtgacgg tgatcgtcgt acggttcctc atcaagcagt
     3121 acaaccgcgt gtccccgaac accgtcgctg tcgtctacgg caagcgcaac ggtgagaagg
     3181 gctatcggat cgtcactggc ggcgggttct tcctctggcc ggtgatcgag gaggtgaagt
     3241 acctcagctt gaacatcatg tcgatcgaag tcgcggtggt gaacgccccc gacaagaacg
     3301 gtgtgctgct ctcggtgaag ggcgtcgcga acgtcaagat ccgttcgcag acggaacatc
     3361 ttccggctgc gattgagcgc ttcctcggca agtccgagga agagatcaag cgtatcgcca
     3421 aggacaactt ggagggtaac ctccgagccc tgaccggtac gctcacggtc gaagagctca
     3481 tccaggatcg cacgaagttc caagcccagg tgctacagca ggttggtgcc gacatggcca
     3541 agcttggcct cgacatcgac ctactgaaca tccagaacat caccgacgac aagggctaca
     3601 tcgactcgct cggccgcaag cagaccgccg gcatccatcg tgatgccgag atcggcgaag
     3661 ccgaagctcg gcgcgagact gccatcaaga cggcagaagc tcgtcaagct ggtgagatcg
     3721 caacatcact cgctgatcag aaggtctcgg acgctcacaa gcaacgagac atggccgtcg
     3781 ccgagaacaa agcacaagtc gaagcggcac aggctcgtat ctcgatcgtg tcccaagcgg
     3841 ccgcggctgc ggagcagaag aagctcaaca tcgcgaccgt cgaagcggaa caggcgcagg
     3901 tcgaagcgga gatcggactc caggagtccg tccgcaagcg gacggaagcc aagctcaacg
     3961 ccacgaccat cgtcgaggcg gagaagggca aggaagccaa gatcatcgcc gcggaagctg
     4021 cgagaagggc gcgtgtcatc gaggcggaag ccacgaagga agcgcgtatc atcgaggcca
     4081 acgctgatca agaagcatct gtcctcgagg gtgaggccag tcgcatcaag tcggagaagg
     4141 agggtctggg cgcacaagcc agaatgacgg cagaagcagc tggtcgcgtc aaggtggcgg
     4201 aagccactcg agcggagaag gaggcagaag cagctggtcg caaggccctg cttctcgccg
     4261 aggctgaggg cgaggaagct cgtctcatgg caacggcgcg cggcactcaa gccactctca
     4321 tggcggaagc agatggtcgc aaggccctgc ttctcgccga ggccgaaggc gcggagaaga
     4381 aggcagctgc cttcgcgaag ctcgatgaag cgagcaaggc gatgctcatc cttgagcact
     4441 tgcccgctat catcggggca ctcggtgatg ctgccagcaa ggtcatcgtt ccggccgcgg
     4501 aagcgatcgg tgaaggcctt ggcaacgtca aggagatccg cctcgtcagc atgggtggcc
     4561 agaacggctc ggtggacggc aacgtcctct cgcagttcat gaaggctccc tctgaggcac
     4621 tcttcggtct cttccagcgg atcaacgccg ctggcctggg gccgatggtc aagtcgatgc
     4681 tcagcaagta tggcatcgat gtctcggatc tgttccccga accagccaag ctggcgggtg
     4741 gaagcgaagt tcccgcaacg gctgccccac tttcgacttc gaaggaaggt tcggcgaagg
     4801 tgggaagcaa gtaacaagtt ccttacgaac atcaggacac tccacacagg aaactgtgtg
     4861 gagttttttt ttataaaatt gtgccggaga gaggactcga acctccaccc cttgcgagac
     4921 acgattttga gtcgtgcgcg tctaccattc cgccactccg gcaatttgtt ctatcaactt
     4981 acaccaaaaa agtgctaaaa tctacccatg tcgcagatat acaatgacgc aattttctgg
     5041 attgaggtag acaagatcca tccaaaccct tatcaaccgc gtcgtgagtt cgatgagtta
     5101 gcactaaaga gtcttgctga ttcgattcgt cagtacggag tacttcaagc actggtggtg
     5161 actagaaaag aagttgaaaa gccagatggc ggcctagcta ctgagtatga acttattgct
     5221 ggtgagagac gcctccgtgc ttctaagctc gcagggttaa cgcaggtgcc agcacaggtg
     5281 cgtaatggtg aggagacaga cttgatgaag ctcgagcttg cgatcataga aaatattcaa
     5341 agagaggatt tgaatgcagt tgatcgtgcg aaagctttcg ataaacttgc aaaagagttc
     5401 ggcttcaaac acgctgagat tggcaagaag gtaggtaaga gtcgagagta tgtatcgaac
     5461 accttgcgca tcctgatgct cccagacgag atgctgaatg ctttggcaga ggggaagatc
     5521 acagagggtc acacacgacc attgatgatg ctcgttgata aaccagagga gcagatgacc
     5581 ctcttcaaag aaataatgtt caaaaagatc aatgtgcgtg atgctgaggg tattgctcgt
     5641 cgcatcgctt tcgaaaaggc tcgcaaactt atggacccag acttggtgga tcttgaagac
     5701 aagttcaaag agactcttgg gacccgagtg cgtatagaga agagagatga tggtggcaaa
     5761 cttacaattg atttcttctc caaggacgat cttatatcac ttctcgagaa actctcgaac
     5821 gaagttcatg tactcccaga tatgatagca agcacgccaa catctgaagt accagtaccc
     5881 acagaggagg agaaagaggt gatgagtgag gggagcaagg aggctatgga ggcttcagac
     5941 gaggatctgt actcaattaa gaacttttcg gtataattaa tactatgaag aaacaatata
     6001 ttcagattgg tgcattagta gtcgttttgg tgggtattgt gcttttcgtg ccatttaata
     6061 agccagagat caaggatctg gaggatatta agacaggtga gttgaagact agtaccaaga
     6121 gtagtacctc tggttctact acagttaaca cccccacaaa tacatataac ccaagtaaca
     6181 aggagttccc atttagcttc acagaaaaga atcttgtttt aggtacacct tttggttcgt
     6241 tcaagtattc tggattcgac tatgcgaatt atcataagtt ttctggtagt gcgactgtct
     6301 ctggtcaggt aactgctgtt aagacagatg gtgaggacaa tctaggcatc actgttgata
     6361 aggctgacat gtacaagctc ccacagtact acttcgcaag tattcaaggt gagaatggaa
     6421 cttataacta cgttcgagct gagaagcctt atgttgtttg cttcggatac tactacccat
     6481 ctcagtacga ttacaacgaa ctactgggca agcttgctct taaggcggga gagagagtta
     6541 ctgtaaagat cgacaactac aagatcatag cttcgaagga acatgtaggt gcctgtgcaa
     6601 gtcttcttga gatagtcggt aattagctag ctattgacaa atagtgtatt tcatgtatac
     6661 taaatacagg tagttcatcg tgctgaaact taaccgggag agttcgagat ggctcgtagt
     6721 cgtcacgacc gaagcaatcg caggaagtcc ttggacttcc agatcagtcg cttgaagcgg
     6781 cgtcggcttc ttgagaggtt tatgatgaag gatctcgacc tcgagcccat cacgatcttt
     6841 cggatccctc gccggcgtag tcgaacgatt aacatcttcg accgcggtcg ccggatccag
     6901 gtgaccctct tccgcgagac cgcgcatgtc ggtttcgtcc cctagaccat ggtcaccacc
     6961 cgtccgccca acctcacaga ggtctgggcg gatttcgttt atagaaaata aaaacgcagt
     7021 atggacttaa ccatactgcg atgtttgtta ctgcgccaca gggacttctt cgacagcctc
     7081 agctgggaag tatatgacga gcatcctaag gatcttattg gcctgctcag gctcgctctc
     7141 cttgtagtgg tgatacagac caataagggc tttcttggga tcggggtgct cgcccattgt
     7201 cgtcttgctg agtactgctt cggcacggcg ctgatttttt ttggctcgaa tagcatccct
     7261 gcgttcgtat ccccaggttg aaatgcttcc agatagtgac ttagcgtcct tttcaacctg
     7321 atcaagctct tcctcagtct gggctgaatc aatcatctgc ttgaagtggt tattcagctt
     7381 gtccaacgcc acttgcctag tcttcgttcc aaaaggagac gactcgagta gctcggccag
     7441 agtgttggcg tcgacactct cttcggctac atagagtttg aggtcgtccc acttgtcgag
     7501 catgatctgt ggaagttctg gccaggagat gtgaggacct gatccaacag aattctttgg
     7561 acgcgatctc cagattttga tgatctcctt aacttgcatt agggtacttg ccttctcagc
     7621 gactagttcc agggatcgag agtggaggta gtctggacca ttattgtctc tttttgccag
     7681 gctgttggcg aaggcgagtc gctgtctgaa cccacgcatg cgacccatgt gcttatcgag
     7741 caacgttgtc atgcgcacct tcaaaccgtc actgctgatg aagtcacccg tcttggtttg
     7801 ctcaacgagg aaagcatctt ccagcgactc aatctcctcc cagacgaact tccactgcgc
     7861 tgagcttttc tcctgccgat gatgttcaaa gccaaacaag tcgtaataac ttattggcat
     7921 tgtcttgatg attttgagtc gatccttgag actcttcgtt gagcgaagtg cttcgatgaa
     7981 gtagcgaatc atcttgtact tcagatcttg ggcaaaacct tgatcgtaga tcttgaagta
     8041 cttgtgaaag tattccacta cttgatgaac ctcgatcaag gcggtgagat cgttggccga
     8101 ctctagcatt tccttgatga ggctgtagag cttggatgat gcagtagacc attcatcatt
     8161 gtaaaaactc agtgatgggc cagctgctat cttgccgaat gacttgaact gctcgatcgt
     8221 actgaactga ccaagatagt ccgagaagaa ttctcctcgg cgtgccttat actcgtgcaa
     8281 ttcttcaggc tcgaacttcg gaccacaact gctgtgcatc ttcttcctaa attctggttc
     8341 gagtgcgacc atgtcgagga aagtcttggc cgctcttacc tggccctgga gccgagtgac
     8401 gataccaagt ttgatgcgta agctatttgg cctgatacca agtgctgtgg ccatgttgtc
     8461 cagtgcgacg tgcattatcc ttctcctgtg atcgaggtgt gttgtattgc aaaggacttc
     8521 cgcgcctatt tctacgcccc ttttgaaaaa aggtcaaact atttttcttt cttcttgaat
     8581 cctctggttt ttttaggttc ctttagaatt tctagtgcac gctcgagcgt gatcttgtat
     8641 acatcgtcgg tcttgagtga tctaaagtcc ttctcatgca caatatatgg tccgaagcgt
     8701 ccaatacttg cagtgatctt ctctcccgtg tctgggtgag tgcctagtac tctagggagt
     8761 gagaggtaga caagtgcgtc ctctatagaa atttcagaca ggttctttgt cttggggatg
     8821 ctcgccttac gagcttttat ctttttcttt ttgtcatctt cgccttcctg tacatatggt
     8881 ccaaagcgac cagttttgac gaatatagac agaccgcttt ctgggttcac tcctatagga
     8941 gtatcggcct taatctcctc gccgttaata gtgagtgcac cagtacagtc ggggaagcga
     9001 gagcacgata ggaagcgtcc accacgtccg agtttgataa tcatgctgct accacaagtt
     9061 ggacacttgt atttcttgtc ggcagtaccg aggtcggttg ctttggccaa cttttcctta
     9121 ctcttcacat ctttgttgaa aggggtatag aagtcggtta atgttttcac ataagttctt
     9181 ttaccaagag ctatgtcgtc tagcttgtcc tccatatctg cggtgaaggt gtcgctaata
     9241 tactcaccga agttgtcctc taggaagccc gagacaacct caccagtgtc ggttggcttc
     9301 agagctttct ggtccttctc tacataacca cgatcaacaa tagttttgat aatagacgcg
     9361 taggtagaag gtcgtccaat accacgcttc tcaagctctt taataagtcc tgcctctgag
     9421 tatctgcttg gcggctcggt ctgcttttcc tcagaaaaga ttttgtccaa atctaatttg
     9481 tctccaacac tcatctttgg aaggatcacc tcctcacctt gtgcagcttt atcggccttg
     9541 gtccatccat cgaaaagtat gcgagagcca ttaagccaga agtcaggaat ggatttatcc
     9601 ttgatatttg ccaccacctt ggtgcgcata actacagcat ccttcatctg ggtggcaaga
     9661 gtgcgggacc aaataagttt gtacaacttc ttctgctcgt cggtgaaacc agcattctcc
     9721 acattcatat gagttgggcg tattgcctca tgagcctctt gagcattctt gctcttcttt
     9781 gagaatacat gtgattcgta atacgactct ccaaatttgc ttatgatagt tgatttgatg
     9841 tctgctctgg cttgcgcgcc taggttggtt gagtcggtac gcatgtaggt aatgtatcca
     9901 gactcatata gcttctgagc aatacccata gtgcgagatg gagcgaagcc aagacgagat
     9961 gacgcggcct gttgtagagt cgaggtaatg aagggggctc gaggtgacct cttgacctct
    10021 gtctctttaa tatctattac actccacgaa cccttttctc cattttttaa aatactctcc
    10081 acctctttct tgtctctggg ttctatagag cattcgaaat ttagtttatt tagttctgca
    10141 aatattttcc aaaatgtctc tggattaaat gctcgtatct cgcgctcccg ctcaaccaat
    10201 atacgaagcg cgggggactg tactctaccg gcggataggc cgtaacgcac cttcttccag
    10261 atgatgccag agaggtcgta acccacaaga cgatcgagaa ctctacgagc ttcctgagct
    10321 tccttaagat tctcgtcaat tagccttggg tgttctatgg cttcttccac agcttctttg
    10381 gtgatttcgt ggaagacaat tctttttggt ttcttaagac ctatggcgtc ggcaatatgc
    10441 caagctatag cctctccctc acggtcgggg tcggtagcaa gtattacctc gctagctttc
    10501 ttggcaattg ctttgatctc ggctatgact ttgtctttgc ccttagacac ttcgtagtgg
    10561 gggacgaagc cgtgaggtat gtctatagcc ttcgcattat tcttcggtaa gtcacgcaca
    10621 tgaccaacag acgccacgac gcgaaagtcc ttacccaaat atttttctat cgtcttggcc
    10681 ttgcttggag actcgactat tactaaacgc atttctcaat taatactacg gatatctttt
    10741 ccatgcaaat ttcgtctatg aaagaatgag agaaggttaa aaagtttttc tcactaatcc
    10801 aagctcttct actataagtc ctttgatctc aagcaaggac attgtggagg taacttggtg
    10861 ggtttcgaga cctgactctc tgactagcac gtctctctcc ttcggcttat ctagtagttc
    10921 aattatcgct ttttcctcat cactcaagtc gtcaatattt agtagcgtgt catgaccctc
    10981 gctccttaga cctaggagtt ccaaaatgtc gtgcgagtcc cttatgagtg ctgcacctaa
    11041 gcggatcagc atatgtggac catcggatgt tgaggagctt ataggtcccg gtactgcacc
    11101 aacctctcta ttatattcag tagcaaggcg tgaggtgata agggtcccac tcttcttctc
    11161 tgcttctata actatcgttg catgacacat gcctgccata attctattcc tcctggggaa
    11221 ggcccatgct ccagagggca tggtgtcgtt gtattctgat attagaccac caccctctct
    11281 tactatttcc tcagctaagg atacatggga gtgcggatgg agtacttttt tatcaagccc
    11341 tgaccctggt actccaatag tctggagccc tgcccgcata gcactcctgt gcgctataga
    11401 gtcgatacct agagctagac ccgagacaat agtgatgggg tagcccgcta gtccttgtat
    11461 aagagaggat gtaacctcct gcccatatag gctgaacttc ctagaaccaa ctatagctag
    11521 aagcttgtta ccctctattg gaggttcgcc ccatagacgg agactctttg gcgggtcgtt
    11581 aatctctttc aagagttctg gccactcttc ctctagaagg tcccgaatca tgtggtaata
    11641 ttctagcaat gttagatatc aaattcatta gagagaacaa ggacctagta gccatggcgg
    11701 cgaagaagaa gcacatcgac tttgatgtag agaagctcct agacgtagat gaaaagagga
    11761 ggagtttgct ccagaaggtg gaggagaagc gtggtgagca aaacatggca agtaaggaga
    11821 tcatgagtgc ttctggtccc gagcgcgcga atattttgga gaagatgaaa tttgttaagg
    11881 aagaattaca aaaaggggag gatgagttaa aggagctgat gaaggattgg caaggcttaa
    11941 tgcttcaagt tccgaatgtc cccgatatct cagtcccaga tggagtaagt gatgagcaga
    12001 atcttgaggt gaagaagtgg ggtgatttac ctaggttcga tttcgagccc aagagtcatg
    12061 tagaacttat ggagaacttg gggatgctag acctcgagcg agggaccaag gttgcgggtt
    12121 tcagaggtta cttccttaaa ggcgatggtg cacgactaca atttgcgctt tggagatttg
    12181 ttcaggatta ctttgcgaag aagagagact atgtgccgat gatagtgcca tctttaatca
    12241 agagagaagt atttttcggt actggttact taccacaagg cgaagaggat ttgtataaga
    12301 cacaagatgg agagtatttg tcaggtaccg ccgaggtagc gaccatgggt tactacatgg
    12361 acgaaatttt ggataagaaa gatttaccca taaagcactt gtcattttct gacgcatttc
    12421 gtcgtgaggc tgggagtcac ggcaaggata cgaggggcat actccgagtt catgagtttt
    12481 ataaatttga acaagtgatc ctttgcgagg cgaatcacga ggagtctgtc aggttccacg
    12541 aggagataca gaggaatgtc gaggagatca ctgaggctct tgggctacct tatagagtgg
    12601 tagttaactg cggaggggac ctaggacttg gccaggtcaa gaagtacgac accgaggtct
    12661 ggctaccttc ggagaagaag tacagagaga ctggctctgc atcttacttc cacgacttcc
    12721 aaacaaggag gcttggtatc cgttacaagg acacagacgg ctcactcaag tttgctcact
    12781 cgcttaacaa cacagctctc tcgggacgac cacttattat gttggtagag aacttccaag
    12841 aggcggacgg cagtatcaag ataccagaag tgttactacc ttatttcggg aaggataaaa
    12901 tcagccgaga tgaaactagt cgggagtaaa ggagctgtcc gctctgcaga gttccacaaa
    12961 aagaaggaga gggagaaaaa gttttatcaa ttacttttta cgctcgcatt tcttttgata
    13021 atagttggcc cgatcttcct ccttaggagc gagaaactca aggtgagcca agttagtgtg
    13081 agtggtaaca tggtgacgca aagtagcgaa gttgaatcgt tggtgaagga gagactgagt
    13141 ggttattatc tcggtctcat acctaagtcg agcacactct tctacccaaa ggatgagata
    13201 gttgatacgc tggataaaca aataccaaga ctcgctagtg cgagtgtctc gcttaacgga
    13261 tttcgaggag taagtgtgga ggttgtagag agagagcccg ctgcactcta ttgtgctcat
    13321 gtggtagaca aagagtcttg ctacttttta gataatactg gctacatata ctctgaggca
    13381 ccggacttca ccgatggtgt gtatctagtt tatagaagag acccgagtat taatgatcca
    13441 gttgataaat atatattgga agagaatgag tttgctagag taaacaagtt tataaatagt
    13501 ttgggcaagc tcgagatgaa gtcggtaagt attttgaaca aagaaggaga ctacatccta
    13561 actttagata atggtcccga gataatgtgg aacagtaatc aagatttgga caaggtcttg
    13621 gtcgacctgg agtcattcct caagggctca aagctcacaa tgagcgatat aaagaacctg
    13681 ctttacttag atctcaggtt cgacaacaag atctactata aggggaggga atagctattt
    13741 gcttgacaat ttctgatagg gggtgtatag tcttaaaaga atgttcattc gggtagttgc
    13801 tagctctgag atagattgtt caacagtcct acggaatgtt gacgagatat caacgaggtg
    13861 tacaaatgac tgtaaagcac gcagatggac caatctggtt caagtgaaag ggttctccct
    13921 caatcttgta ctaggactaa tgggaaccgg ccccggtctc cctcacatcg caaatggtcg
    13981 acacactgcg gatcgctaac cttcatttgt ctcggggaaa tcgatacttg aattcagggc
    14041 ctccgaaagg tgcccagagc aagcctccta gcaacttccc accccccttc accctcgaca
    14101 tgttcgaggg tgttttcatg tttagtacaa tacacatatg aatttctgga agaatcttaa
    14161 gagaccaata tttgttctag cccccatggc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnatgaattt ctggaagaat cttaagagac caatatttgt tctagccccc
    14341 atggctgatg ttactgactc ggcttttcga gctattatcg ccaagtatgg taagcccgat
    14401 gttacttgga ccgagttcgt ctcggctgac ggcctggcac tcgcacccga ggaaggaagg
    14461 aaaaaacttt tgaaagatct tgaatttact aacgcagagc gtcctatagt tgcacaattc
    14521 tttacatcaa aacctgagaa catgcggaag gcagcagctc ttgccaagga acttggtttc
    14581 gacggtatag acatcaacat gggttgtcca gatagaagta ttgagaagca gggtgcagga
    14641 gcagctatga tgaaaaatcc aaagctcgca agagaggttc ttagagccgc tatagaaggc
    14701 gctgccggat tgcccgtgtc ggttaagact cgtgttggct ataacaaggt ggagttggaa
    14761 gattggttac ccgagatact caaagaagat ccagctgtag tgactattca tgctcgtaca
    14821 aggaaggaga tgagtaaggt tcctgctagg tgggagttcg tgaagcgtgc tgtagagata
    14881 cgtaacgaac ttgggtcagg tacgttgata cttggtaatg gtgacgcctt gagtatcgaa
    14941 gatgctaaga aaaaagtaga tgagacagga gccgatggag tgatgctcgg tcgcgcgata
    15001 tttggtaacc catggctttt tagtgagaag actccgactt tggaagaaaa gcttatagtt
    15061 tgtgtcgaac atacaaaatt atttgaaaaa aaattaggtg ggttaaagaa ctttgctcta
    15121 atgaagaagc actacaaagc atatgtgaat ggttttgatg gagcgggaga cttgagaggt
    15181 aagctaatgg aagcaggtaa tgcagaagag gtagaggaca ttgtaaaaaa ctttttgtat
    15241 aattaagctt cacccaagca tgaggtggtg catggcagcg aagaggctca ccaggtggaa
    15301 gatcgcgctc ctgaacaaga agggcaggaa gagcaacaag aagaagggcg gcggcgcgaa
    15361 caacgccgga ggctccacct gaacgcgatg tagggcaggg tgccagtacg gagggtttgg
    15421 gggggactcc gtactggtta ctctggcttt caaaccgcct tagggtagat acactacgac
    15481 catttggtcg tttttttata tttaatttag acttgacaaa caatttaagt aggtttataa
    15541 tagcaagtag attacagaag tagagaattg tgttgtggca caagtctgtg gtcacggtag
    15601 tggtgtgtgg tgagtcgtcg aggcccggaa tcctcgccgt tccacccatg tcctaccgtg
    15661 gcccagacgg ccatatccta tctactccta gatctttctc taggccccaa ttctggggcc
    15721 tttctcatat ctgaatttcc agtgaattta cctttgacaa aagctttctt ttgtgataat
    15781 aagagggcag aaacagctct tcgaaaatca catttcttac actaggagca ccaatgcgaa
    15841 ccaggtggtt tccgaggctg gtgaccttct tggtcgccat cctcatgttc tccgcttgcg
    15901 gagacaacaa gcagatcacc aaggtgatcg tgaacatctg gctgccgctt ggcacccgga
    15961 ctgataccac gggcacctgc gtgaggacta cgatgatcgg aacctccgac acatcgaacg
    16021 ttgcagggtc gaacatcctc ttgccacgag gcgaggcgga ctcactttcg gtgagtcagc
    16081 gcttcaaccc gagcggttgc ggtacgcctt atacgttcag gtggagcacg agtaacgcga
    16141 gtgtcgtctc cgttactgcc tttgactcag tctctgctcg catcacagcc atctcttcgg
    16201 ggacagcagt gatcactgcg acagctgtcg aggacagtac catcgtcctt agggttactg
    16261 tggttgtgcc cggagtgtct acgcctaccc cggtctcgat ttcggttgag ccggtgagcg
    16321 cgactctcta ctctgggcag accatgaccc ttggcactgg tacctaccgt gtggtggtga
    16381 ccatgtccga cggaacgacc agggtcaacg acctgtccac cttctcctgc tcgagtgcaa
    16441 acacgagtca ggttacggtg aactcctctt gcttgatctc ggcgctcgcg ccgacgacct
    16501 cgggtggagt ggtggttact tacgccgcgc tcggagtcac tggggtgacc gacacactcg
    16561 tcgtgagtgt gaatgctctc cccgtgatca gtttgaaccc cgtgtcactc tgcttcagca
    16621 ctgtggtcgg gactccacaa tctcagactg tgacggttac cgtcacagga gcgagtgtga
    16681 gcaatgtggt cctcgcgtcg aacaccactg gtgtcactgt ctctggttca ggttctacct
    16741 ggactgtgac gaggacggca ggaagtgttg tggcgaacgg gagcttggtt gcgactctca
    16801 ccacgatgag tggtgtggtc acggctatcc cggttcagat tatttctacg gtgtgtcctg
    16861 ggacgacgag ttccatcgac ttcaccccga gtggtggccc gcttccgatc ggactcgata
    16921 ctctgaccgc tacttgcgtt cagggcgccg agatcccgaa tgtgtgcgag ccgtactgga
    16981 cgtcgtccga cccgagcatt gcgacggtga gaggtaacac agcgaagcct gttggcccga
    17041 tgaccttctg ggttggcctt atcgcctacc tcgagcgtct ccggcccggc acggtcgaga
    17101 tctgtgtgca gtggtccctc actcagacga gcccgcgtag gtgttacacc ttcacgagcc
    17161 cgtaagagcg aaccccagag acaagttatc gtctctgggg tttttcattt ataatgttca
    17221 acatgtcagt cctctatagg aagtaccggc ccaagagctt tgatgatgtt atcggccagg
    17281 aaccgatagt taatgttttg aaaaacgaga tcaagctcgg cactcttgct catgcgtatc
    17341 tcttcgctgg ctcgcgtggt acaggcaaga cctcagtagc caggatcttt gctaacgagg
    17401 ttgggacttc gaagagcgat atttacgaga tagatgccgc gtcgaacaac ggagtggatg
    17461 agatccgagc gctgaatgac gcagtgcaca ccctgccatt cgattctaaa tacaaagtct
    17521 atatattgga cgaggcacat atgctctcta agggtgcgtg gaacgccttt cttaaaacac
    17581 ttgaggaacc tccagcgcat gtcatcttta tcctcgctac aacagagtta aacaaagttc
    17641 cagagactgt gcagtctagg tgccagtcat tcgtattcaa aagaccaagt caaaagatct
    17701 tgcgtgagtt ttcacaaaag attgcaaagg atgaggggag ggtacttgct gacgacagcg
    17761 cagagttggt tgcgctactt ggtgacggat ccttccgcga tactcacggg atactggaaa
    17821 agattttcag tagtaccaag gagaagaaga ttgagaggga agaagtcgag atggttacag
    17881 gtgcgccaag gggagagctt gtaaggagtg tgctcgaggc acttataaat gaagacctag
    17941 agcaagggat gaaggcgata gctgaggcga gagagaacaa tgttgaggcc aagatttttg
    18001 ccaagctcat tctcgagaga ctgcgttatc ttttcctcct cagacttaaa gcagggatgg
    18061 aggattatat atcgagcgag acctcagatg acgaattcga gttcttgaag accctagctt
    18121 ctaaacctgc caagcacctc acctccgaag tgctcctccg ctttatcgaa gcgtctgagc
    18181 aatccggccg atcttccata cccgaact
//
LOCUS       DAORIT010000011        17487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_541_length_17487_cov_52.336508, whole genome shotgun sequence.
ACCESSION   DAORIT010000011 DAORIT010000000
VERSION     DAORIT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17487
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_541_length_17487_cov_52.336508"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..525
                     /locus_tag="PLD99_01545"
     CDS             277..525
                     /locus_tag="PLD99_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25621.1"
                     /translation="MLSILLGWLAGYFLTVPLMVLISVGCLIIVIIMSIRLQEMAGLV
                     TVIFAALASIGNLVMWATYYIVTDQTFLGDFVQKYITR"
     gene            complement(657..863)
                     /locus_tag="PLD99_01550"
     CDS             complement(657..863)
                     /locus_tag="PLD99_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25622.1"
                     /translation="MSLENLNFHGDPSLPSFKFTVERPEMLKKLIEGGMTDEEAEAEV
                     SRKEAEITNKQAQARFRKENKRAA"
     gene            complement(860..1738)
                     /locus_tag="PLD99_01555"
     CDS             complement(860..1738)
                     /locus_tag="PLD99_01555"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25623.1"
                     /translation="MRIAVLRGGPSKHYDDSLMTGETVLSVLRDEPKYKPLDIFVSKD
                     GEWHHMGRRYEPHHLLRHVDLVWNAMHGEFGEDGKLGQLLSQMNVPHTGSTSLHLAFS
                     LNKDLAKKVFDQHNLLTPKHEVLLGTISNDDLVRIFQTYLHPVMVKPVSTRSSLGERV
                     AHSFNELKDSVVEAFKHAERVIVEEFIKGDSAKCGVVENFRGKSLYTLIPTPSTFKSN
                     VHKEIERMSAEAHRALGLRHYSTSEFVVTPKGKVYILETTALPPISPETHMHQSLGSV
                     GITPKEFVNHVINIAI"
     gene            complement(1757..2983)
                     /locus_tag="PLD99_01560"
     CDS             complement(1757..2983)
                     /locus_tag="PLD99_01560"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRH25624.1"
                     /translation="MNSQDYKKANLPDAPGVYFFLGPRKEILYVGKATSLKDRVKSYF
                     SNDLNKTRGPLLVKMIDEAKTIDFTTTDSVLEALLLEASQIKQWRPPYNSRDKDNKSF
                     NTVVITDEEFPQILVVRARNLEHWEKISKDLKIRSYFGPFPHGGELREALKIIRKIFP
                     YRDEKCNLKKKCFNAQIGLCPGVCAGLMTSKEYKKVVRRIELFFNGKKQDLIKDVEKE
                     MKSLAKGQKFEEAEMQKRTLFALEHIHDMALIKRDIERGDSANTTRIEAYDIAHLSGK
                     DTVGVMVVVEDGEVAKSQYRKFKIKVDSNDDTNNLREIIRRRLGHLEWDLPRIIVVDG
                     GAGQINAARDEIKKRGFDIELVAVVKDDKHKAREIVGEKALISKWGKSILLANTEAHR
                     FAIGYHRKLRNKGFRI"
     assembly_gap    3022..3121
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3122..3348
                     /locus_tag="PLD99_01565"
     CDS             <3122..3348
                     /locus_tag="PLD99_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25625.1"
                     /translation="DQSLRANMPKMKKYRKRNTGTLGKKKVRSKKLKNLRMSDHYLNR
                     EYFDHKVNTPHGRKKAIWKTRWNQARTRSY"
     gene            complement(3345..5990)
                     /gene="uvrA"
                     /locus_tag="PLD99_01570"
     CDS             complement(3345..5990)
                     /gene="uvrA"
                     /locus_tag="PLD99_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRH25626.1"
                     /translation="MHMSKDKKNDTEGKIIVRGARTHNLKNITVEMPRNKMVVFTGLS
                     GSGKSSLAFDTIFAEGQRRYVESLSSYARQFLHQMQKPDVDEIIGLSPAISIDQKSRS
                     NNPRSTVATITEIYDYLRILYARIGRPHCLVCGREIQKLSNEEIKNFILDKITEKAKT
                     ITKGKKIDLPVYAEIFSPIVRGRKGEYYQLLYDLLGKGYIEVLVDGKRHNLRERIELT
                     KTKTHSISVLVDAISLGDFENDTKSANERLSEAVEKAVHESEGLVVLRFRDLASEQKG
                     KDKKTDIDDTEFLMSAKWSCPHDGFSFPEIEPRLFSFNSPYGACPECNGLGTKYFFGT
                     EPCPKCKGNRLREEALNVLVGGKNIVDITKLSIKESEEFFKKLKLTVREKSISKVVTK
                     EIESRLDFMLNVGIDYLSLDRRAHTLSGGEAQRIRLASQLGSGLVGALYVLDEPTIGL
                     HARDNDRLIKTLVNLRDLGNTILIVEHDEDTIYSSDYIVDIGPGAGRHGGEVVVSGDL
                     EELLTAKKNPSGSLTLSYLRGETEIEIPDSRRESEKGVIQIRGGNIFNIKHMNVDIPL
                     GRLISVTGVSGSGKSSFVYEILYKNLQARLEKRYRSAETYNAQSFAGSEYVSRAILID
                     QSPIGRTPRSNPATYTGAFTFIRDMFATSEEARARGWKANRFSFNVKGGRCETCQGNG
                     LIEVEMHFLPTVYVPCDVCQGKRFMKETLEVKYKKKSIYDVLEMTVEEAINFFEDIPA
                     IYDRLKTLGDVGLGYLKLGQSATTLSGGEAQRVKIASELYRPHIAKTIYILDEPTIGL
                     HYEDVKKLIEILQKLVEKGNTVLVIEHNLDLIKSSDYIIDIGPEGGDGGGKLVAKGTP
                     EEVANTDTHTGDYLRRILRKKKRTT"
     assembly_gap    5994..6093
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6423..8465)
                     /gene="uvrB"
                     /locus_tag="PLD99_01575"
     CDS             complement(6423..8465)
                     /gene="uvrB"
                     /locus_tag="PLD99_01575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRH25627.1"
                     /translation="MSGFKIKREWEPAGDQPKAIKALTEGVKKGHKYQTLLGVTGSGK
                     TFTAANVIQNIGKPTLIIAHNKTLAAQLAQEYREFFPENAVHYFVSYYDYYQPEAYMP
                     VTDTYIEKEAMINEEIDRLRHATTQALLTRKDVIIVASVSCIYGLGSPEEYQKVNIKI
                     EVGQSISRSDFVRELIRIHFSRTNSDVKPGTFRALGNAIEIMPTGERMIYRVEIDGGM
                     IKSIKRIDAVSMSIISEPNFYFIFPAKHFITQDDQAKRALLSIKQELEEQLKVFAKEG
                     KILEAERLKRRTTYDLAMIKEIGYTSGIENYSRHFSNRGPGEAPDTLLSYFPHKADGT
                     PDFLTIIDESHVTLPQIGGMHAGDQARKKTLVEHGFRLPSAKDNRPLTMDEFKERVGQ
                     IVFTSATPSDFERENSAQIVEQVIRPTGLIDPVVTIRPINERGDYRGQIFDFIEEAEK
                     VIEKGGRAIATTLTKRMAEDLSEYLKEKKIKASYLHSDIKTIERIEILTAFRRGEFDV
                     LVGVNLLREGLDLPEVTFIGILDADKEGFLRSETSLIQIIGRAARNVDGKVYLYADNI
                     TGSIERAINETNRRRTIQLAYNKEHGITPKTIMKKIKDITDTLEREHGQAVKTLLSTD
                     EEMFKKNPKKLIKDKMKEMEDAVENLDFETAAIIRDEIKVLESRTEIKKDRKKKAA"
     assembly_gap    8467..8566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8567..9702)
                     /gene="tgt"
                     /locus_tag="PLD99_01580"
     CDS             complement(<8567..9702)
                     /gene="tgt"
                     /locus_tag="PLD99_01580"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRH25628.1"
                     /translation="MAPLSFGILKKINGALGRAGVIETPHGKINTPAFVSVGTKGTVK
                     AITPEQLLDLGAEVVLANTYHLYLDPGPERMKNFGGLHKFMNWSGPTMTDSGGFQVFS
                     LGAAYGKELSKVVSITDPRQMLAERSLDGETPSLAKIGADGVSFRSHHDGSLHYITPE
                     KSIDIQHHIGADIIFAFDECTSPSETFAYQEEALERTHRWAKKSLEYHKSKPNAETQA
                     LFGIVQGGREESLRKKSAEFIGSLDFDGFGIGGSFAKEDMETAVKWVNEILPEEKPRH
                     LLGIGEPEDLLMGIENGVDLFDCVAPTRNGRGGTLYTHQGKVNIRNAEFRDDTNTLDS
                     ECGCYVCKNYSRAYLCHLFRTHEMLGGTLGSIHNLYFIINLVKRA"
     gene            complement(9702..10721)
                     /locus_tag="PLD99_01585"
     CDS             complement(9702..10721)
                     /locus_tag="PLD99_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRH25629.1"
                     /translation="MISLQKKLGIDLGTTNILVFVPGKGIIMNEPSVVAVSISDNKIL
                     AIGAEAKTMVGRTPEDIVAYRPMKDGVIADYRVTEAMLRYYINKALGKWNIFKPDVLV
                     SVPAGVTSTERRAVIEATLKAGAKNAYVVKEPILAAIGAGIPIQEAAGHMIVDIGGGT
                     TDVAVISLGGIVASTSVKCAGNKVDSAIQDYIKRTHNLSIGDKTAEEIKIKIGSAVIL
                     QAEDELSMQVKGRDTILGLPRTIELKSNEITKAISKELREMVGAIKDVFQNTPPELSS
                     DIIDKGIIMTGGSSQLRNFPELVLRRTGVKAVLAKEALYCVAKGTGVALEHLDVYKKS
                     IITKR"
     gene            10757..12622
                     /locus_tag="PLD99_01590"
     CDS             10757..12622
                     /locus_tag="PLD99_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25630.1"
                     /translation="MKVVEVVPIVKGFTRETLSYFSKESFAPGSFVKIPLRNGSILGI
                     VESTVDVSQKKSLIKKAGFPLKKISLAPKQALSRGFMKAVETTSKFYATSSGSVLGTL
                     LTKFFLDNPNFLKKNSRTNSETSEVKLIQLSEEERYREYRGVIRESFAKGSSVMFIAP
                     TKKDLDKAKNSLNVGIEKFVYSTAGITPKGLKKLFLKIHEEKHPVLIITSMSNCVFDR
                     PDLDLIIVERENSRAYRGLKRPYLDYKMFLKNYTKECGKSLIFGDSVLSLETLWLEKE
                     GMYSAVTPLTWRLKDKASGEIIDMREKKEFEIISEKLQKRIKMSLTENKKIFLFGVRK
                     GLSSTTACGDCGSLLLCKNCSSPLVLHTQQIQGETLNYYKCHHCGAKRTAETRCDSCQ
                     SWKLTPLGIGIDTIAKECREKMPEVNVFILSKEHTPTKSKAVETVKRFFDTPQSILIG
                     TEMAIEYLESVPTVGVVSLDSLFSIPNFYINERIFYLVTRLKEITGESFLIQTRNAGV
                     NILEWATAGNILDFYRSEIEEREDLHYPPFSYFIKISFEGTIDKVEAKSILLKNDFEL
                     FNPHFMIKKSGQGKTAELTMIIRISRENWPDESLQRKLLLLTPDFLIKVDPESIF"
     gene            12624..13151
                     /gene="def"
                     /locus_tag="PLD99_01595"
     CDS             12624..13151
                     /gene="def"
                     /locus_tag="PLD99_01595"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRH25631.1"
                     /translation="MPKTNILQKDSPILRKVALDVPENEISSQRIKTIISKMKIAVHA
                     EDDGVAIAAPQIGVSLRIFVVAGRTFNIGKKADKETVFPDLVFINPEITKLSKKKKIM
                     EEGCLSVRWLYGKVERHERVSIKALDETGRKIERGASGLLAQIFQHEIDHLDGTLFID
                     KATDIEDVPPTHQDE"
     gene            13123..13863
                     /locus_tag="PLD99_01600"
     CDS             13123..13863
                     /locus_tag="PLD99_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRH25632.1"
                     /translation="MYHQHIKMNKLDFIFFGSSKFSEYVLNVLRDKGYEPKLCITSAK
                     GDLPEIPDADLYIVASFGKILKDELINKPKYKTLNVHPSLLPKLRGPAPIQGAILSGE
                     KTGVSIMRMNAKMDQGPVLATKEVIFENNPEHYVIAEEILGTIGGELLAEVIPGWLSG
                     EIKEKPQDDSQATYTKLIKKEDSDITNDSPEVAMRKIRAYEVWPRARLGELIVTDAHI
                     EGGKLVLDKVIPPGKKEMVYTDYLRGKH"
     gene            complement(13864..14238)
                     /gene="raiA"
                     /locus_tag="PLD99_01605"
     CDS             complement(13864..14238)
                     /gene="raiA"
                     /locus_tag="PLD99_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRH25633.1"
                     /translation="MKTNIKATNIELTEAIRDYALKRFSKLEKYLGNKDVLLSIELGK
                     TTDHHKHGDIFRAEARLSGAGMDNYVAQEADDLYRAIDLVETEVIQDVTRETSKKREL
                     LRSGQRAIKNMIQGFPWIKWRR"
     gene            complement(14251..15636)
                     /locus_tag="PLD99_01610"
     CDS             complement(14251..15636)
                     /locus_tag="PLD99_01610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRH25634.1"
                     /translation="MIPNTSPWIKQLNRSRPAVPLDKDIETDVTIVGGGIAGVVTAYF
                     ALKDTKHRVVLIEADKVAHGATGHNAGQLASYFERPLPGMVEEFGEKLATDGVRDVES
                     AWLLLDEMVEEAKLRTPIYRFTGYAGVAGMSKLVDFLESNKYRERNGLQRESIIISES
                     WDGLKDISDEYKEYYSVAKHKDILDLLDTNNSDYIACISYQKGCTNSALLTEELVGYL
                     SATYKDRFSFFEESPLKIVRLDKESAVLEVLNKNIQTKRVVLCTNGFENFSIDNNVGQ
                     DINTEFHHSVAGRIGYMAGYIQPLNHPPTAISYFSKSEINSNDPTGDTYFYLTRRPHE
                     NEGEKDRDFNLICAGGPEKVLPNGALYSRAHACDEDVQDKINDFLLANYGKHQKETEY
                     AFCWHGLMGYTPNGIRRIGPEPLNPVLLYNLGCNGVGILPSIFGGKKISKFLNNDEVA
                     PSIFDPHDQSK"
     assembly_gap    15704..15803
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15804..16378)
                     /locus_tag="PLD99_01615"
     CDS             complement(<15804..16378)
                     /locus_tag="PLD99_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRH25635.1"
                     /translation="MSLDKTHKSYLIGLIALLLIAVFGLWNLYSKNLDLNTSLTETRE
                     TLGKTLEENLKLTDDLKLARGDIDEYSSQIDGLSSTLEKLKKLAETDRELLKQYSKVY
                     FLNENYVPRGLTDISSVFLSNPAKPQQILALVYPFLQRLLEEADIDGMDLKILSGYRS
                     FESQTSLKNAYTVTYGAGTANKFSADQGYSE"
     gene            complement(16465..16680)
                     /locus_tag="PLD99_01620"
     CDS             complement(16465..16680)
                     /locus_tag="PLD99_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25636.1"
                     /translation="MESVRQIQESAFWSGLTEAVNTLHNTLQRKPKRLRIVFTDNPGS
                     ILNAYREGDLTFKQAVKQLEKWKRSTR"
     gene            complement(17059..17487)
                     /locus_tag="PLD99_01625"
     CDS             complement(17059..17487)
                     /locus_tag="PLD99_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRH25637.1"
                     /translation="MTTINPWINFNGNAEEAFTFYKSAFGGEFTKIVRFKDLASEEFK
                     VPENEENKIMYIALPIGGGSILMANDVPEFMGKINERENRSKIVVSAESKEEAEKLFK
                     TLSLGGEVEGQMGNSPWGTYAGMFRDKYGIEWIIEFSSKE"
BASE COUNT         4554 a         4107 c         3459 g         4967 t
ORIGIN      
        1 tccagagttg cggggcagga cggaccttcc gagaccttgc taacgacata agccaatcca
       61 tggcacttgg ccttgtcaca ataggacccc acaccagagc gtgtcggggt cctctatttt
      121 tatgcaatag acaaatcacc cccccaataa ttaggtggtt atcggtgtga atccggttta
      181 ttcataacaa cttgacaatg aagttagttt atgataataa cttatctgga tttcagatct
      241 acaaacccta caccagaggc tctgatgctg ttgattatgc tttcgatcct gctcggctgg
      301 ctagcgggat acttcctgac tgtacctctc atggtcttga tcagcgtggg ttgcctgatc
      361 atcgtgatca tcatgtccat caggctccaa gagatggcag gactggtgac ggtaatcttc
      421 gctgcactcg cctcgatcgg gaacctggtc atgtgggcga cgtactacat cgtcactgac
      481 cagacatttc ttggtgattt cgtccagaag tacatcacca ggtagatacc gcgagaagtc
      541 gcgagacgta agtctccccg cttctcgcgg ctttttgttt attcaacaca aaaccacaca
      601 atgcaaagag atgatttgtt ttgttactct aagattttct cacttagcaa gaaaaattag
      661 gcggctcttt tattctcttt cctaaagcga gcctgagctt gtttgttagt aatctctgcc
      721 tcctttcttg atacctcggc ttcggcttct tcatctgtca tacctccttc tattagtttc
      781 tttaacatct ctggcctttc aactgtaaat ttaaagctgg gtaaacttgg atctccatgg
      841 aagtttaaat tttctaaact catattgcta tatttataac atggtttaca aactccttag
      901 gagtgatccc tacactaccc aaagactggt gcatgtgagt ctcgggtgat atgggaggca
      961 gggctgtggt ttctagtata taaaccttac cctttggggt gacaacaaac tcagatgtag
     1021 agtaatgtct aagaccaaga gcacggtgcg cctcggccga catcctctct atctccttgt
     1081 gtacattgga cttgaaggtg cttggagtag gtatcagagt gtacaaggac ttacctctga
     1141 agttctctac cacaccacac ttagcgctat caccttttat aaactcttct acaattactc
     1201 tctctgcatg tttgaaggcc tcgaccactg agtccttgag ctcattaaag ctgtgcgcca
     1261 ctctctcacc aagactactc ctagtagaaa caggtttgac catcacagga tgcaaataag
     1321 tttggaagat cctaactaag tcatcattac taatagtgcc tagaagcacc tcgtgcttag
     1381 gagtaaggag gttgtgttgg tcgaatactt tcttggccaa gtccttattc aaagagaagg
     1441 caagatgaag cgaggtggaa cccgtgtggg gcacgttcat ctgcgagagc agttgcccaa
     1501 gcttgccatc ttcaccgaac tcaccatgca tagcattcca caccaaatcc acatgtctca
     1561 gcaagtgatg tggttcgtac cgtcttccca tgtggtgcca ctcaccatcc ttagatacga
     1621 aaatgtccaa gggtttatac ttaggttcat cccgaaggac cgaaagtacg gtctcccccg
     1681 tcataagaga gtcgtcatag tgctttgatg gaccgccgcg cagaactgca attctcattc
     1741 aaaaattata acagagttat atcctaaatc ctttgttcct gagcttcctg tgataaccaa
     1801 tagcaaacct atgcgcttct gtattggcta ggagtataga tttaccccac tttgaaataa
     1861 gggccttctc cccgactatc tctctagcct tatgtttgtc atccttaaca acagcgacta
     1921 gctctatgtc gaaacctcgc ttcttaatct catctcgagc agcatttatc tgtcccgcac
     1981 ctccatcaac tacaataatt cttggcaggt cccattctag gtgaccaagt cttcttctaa
     2041 taatctctcg aagattgttt gtatcgtcat tactatctac tttaattttg aacttcctat
     2101 actgactctt ggcaacttcg ccatcctcta caacaaccat cacacccaca gtgtccttgc
     2161 ccgagaggtg cgcaatgtca tacgcctcta ttcgagtagt gttggctgaa tcccccctct
     2221 cgatatctcg cttgataagt gccatatcgt gtatatgctc gagagcgaag agtgtcctct
     2281 tttgcatttc tgcctcctcg aacttctggc ccttggccag agacttcatc tccttctcca
     2341 catccttgat tagatcttgc ttcttaccgt taaagaataa ttctattctt cttacaacct
     2401 tcttgtactc cttactggtc ataagtcctg cacatacacc tgggcagagt ccgatctgcg
     2461 cgttgaaaca cttcttcttc aagttacact tctcatctct gtaagggaag atctttcgaa
     2521 tgatcttaag tgcttctcgt agctcgcctc cgtgtgggaa agggccgaaa taacttctga
     2581 tctttaagtc ttttgaaatc ttttcccaat gttctaagtt cctggctctt acaacaagta
     2641 tttgagggaa ctcttcgtca gttataacta cagtgttgaa gctcttgttg tccttatctc
     2701 ttgagttgta tggaggcctc cattgcttga tctgagatgc ctcaagcagg agagcctcga
     2761 gcacagaatc tgtggtggta aagtcgatag tctttgcctc atcaatcatc tttaccagta
     2821 agggaccacg agtcttgttt aaatcgttgc taaagtagct cttaaccctg tctttaagtg
     2881 aggttgcttt acctacatat agaatttctt tcctaggtcc aaggaagaaa taaactcccg
     2941 gggcgtctgg gagattggcc tttttatagt cttgactatt cattttttgt attctactat
     3001 attttcagac cccgaacaaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 ncggaccaat cactgagggc aaatatgcca aagatgaaga agtacaggaa gaggaacact
     3181 ggtaccctcg gcaagaagaa ggttcggagc aaaaaactca agaatctccg aatgagtgat
     3241 cattacttga atcgcgagta tttcgaccat aaggttaaca cccctcatgg tcgtaaaaag
     3301 gctatttgga aaacacgttg gaatcaagcg cgcactaggt cttactaagt agtgcgcttt
     3361 ttctttctta aaattctgcg caaatagtct cctgtgtgag tgtcggtatt tgccacctct
     3421 tcaggagtac ccttggctac caacttacca ccaccatctc caccctcagg accaatatca
     3481 ataatgtagt cagaactttt tataagatcc aaattgtgct cgattactag tacagtgttc
     3541 cctttttcta ctagcttctg aagtatctca ataagtttct tcacatcttc gtagtgtagc
     3601 cctattgtgg gttcgtccaa tatataaatt gttttagcaa tgtgaggacg atagagctct
     3661 gaagcaatct tcaccctctg tgcttcacct ccggaaaggg ttgtggctga ttgaccgagc
     3721 tttaggtatc cgagtcctac atctcccaag gtttttaacc tgtcgtatat tgcaggtata
     3781 tcctcgaaga agttaatagc ctcttcaacg gtcatttcca aaacatcata aatactcttt
     3841 ttcttgtact ttacctccaa ggtctccttc ataaacctct taccctggca cacatcgcag
     3901 ggtacataga cagtaggtag gaagtgcatc tcaacctcaa ttagtccgtt accctggcag
     3961 gtctcgcacc taccaccttt aacgttgaag gagaatctat tggccttcca ccctcttgct
     4021 ctcgcctcct cagaggtggc aaacatatcg cgaatgaatg tgaaggcccc agtataagta
     4081 gctgggttac tcctaggtgt cctacctatt ggagactggt ctataagtat cgcgcgcgag
     4141 acatactcac taccagcgaa gctctgtgca ttgtaggtct cggcacttct atatctcttc
     4201 tctagtcttg cttggagatt cttataaaga atttcataga caaaagagga cttaccagaa
     4261 ccagacaccc cggtcacaga tataagtcta ccaagcggga tgtcgacatt catatgcttg
     4321 atgttgaaga tgtttccacc tcttatctgt attaccccct tctcactctc tcgcctgctg
     4381 tctggaatct caatctctgt ctcgccgcgc agatatgaga gtgttaatga gccggaagga
     4441 tttttcttag cagttaaaag ctcctccaag tctccagata cgacaacctc gccgccatgc
     4501 ctaccggcac ccggaccaat gtctacaata tagtcagaag aataaatagt atcctcgtcg
     4561 tgctcaacga taaggatagt gttaccaaga tctctcagat taacaagtgt tttgattaat
     4621 ctatcgttgt ctctagcgtg tagaccaata gttggctcat ccaaaacata gagtgcaccg
     4681 acaagtccag agccgagttg cgatgcaaga cgaatacgct gtgcctctcc accagagaga
     4741 gtgtgtgccc gacgatctag tgagagatag tcaataccga catttagcat aaagtcgagt
     4801 ctagactcga tttctttcgt tactaccttt gaaatacttt tctcccttac agtcaactta
     4861 agcttcttaa agaactcctc ggactccttt atagagagct tcgtgatgtc gacaatgttc
     4921 ttgccaccaa ccaaaacatt gagtgcctcc tctcgaagtc tattgccctt acactttgga
     4981 catggctctg tgccgaagaa gtatttggta cccaagccat tacactctgg acacgccccg
     5041 tatggcgagt tgaatgagaa gagtctcggt tcaatctcgg ggaacgagaa gccatcatgc
     5101 gggcaggacc acttggcaga catgaggaac tcggtgtcgt ctatatcggt cttcttatcc
     5161 ttgcccttct gctcgctagc caggtctcta aatcggagta ctaccaagcc ctcagactcg
     5221 tgaaccgcct tctcaactgc ctcacttaac ctttcgttcg ctgacttagt gtcattctca
     5281 aagtcgccaa gacttatagc atccaccagg acagagatgc tgtgtgtctt tgtcttagtg
     5341 agttcgatcc tctcgcgaag attatgtctc ttaccatcaa ccagtacctc tatataaccc
     5401 ttaccgagta agtcataaag aagttggtag tactcaccct tacgccccct cacaattgga
     5461 gagaagatct ctgcatatac tggtaagtca atcttcttgc ctttagtaat tgttttcgcc
     5521 ttctcagtta tcttatccag aataaagttc ttaatctcct cgttcgataa cttttgtatt
     5581 tctcttccgc aaacaaggca gtgtggacga ccaatgcgtg catataaaat acgaaggtaa
     5641 tcatagatct cagtaatagt agctactgtc gaacgcgggt tgttggagcg acttttttga
     5701 tctattgaaa tagctggaga gaggccaatg atctcgtcga catcaggctt ctgcatctga
     5761 tgcaagaatt gacgcgcgta ggaagagagg ctctctacat agcgcctttg gccctcggcg
     5821 aagattgtgt cgaaagcgag cgacgactta cccgagcccg aaagaccggt gaagaccacc
     5881 atcttgttcc tcggcatctc gaccgtaatg ttcttaaggt tgtgagtgcg tgcgcctcgc
     5941 acaataatct tgccctctgt atcgttcttt ttgtctttgg acatatgcat atgnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgtgcat tactttatgg acacagagtg
     6121 taataggtat actcttaaat tagagctgta cccgttagac tccctacaac cccggtgcct
     6181 caatgtcacc aagcgctcgc aggtatatgt cttgcagacg caagatcact cacctcacgc
     6241 gcaaagaagc atgggatcac gccagacgag ttggtgatgg caacaagtgt tatccgtgca
     6301 gttactgtgg tcacttccac gttggacacc caacacgtga cccacatctt cgagccaaga
     6361 gagtcaaacg cattctcaag cgattcgcgg gcaagaaagc aagattcacc cgagctgcct
     6421 agttaggcgg cttttttctt tctatctttt tttatttctg ttctactctc taaaactttt
     6481 atctcatctc ttataatggc ggctgtttcg aagtccagat tctccacggc atcttccatc
     6541 tccttcatct tgtctttgat aagtttcttt ggattctttt tgaacatttc ttcatctgta
     6601 ctgagcaatg ttttcaccgc ctgaccatgc tctctctcta gagtgtcagt aatatcctta
     6661 attttcttca tgatagtctt tggagtgatg ccgtgttcct tgttgtatgc aagttgtatc
     6721 gtgcgtcttc tatttgtttc gttaattgct cgctctattg aacctgtaat attgtctgcg
     6781 tagagataca ccttaccatc cacatttctt gcagcacgcc caatgatctg tataagagag
     6841 gtctcagatc ttaggaagcc ttccttatct gcgtctaaaa ttccaataaa tgtcacctca
     6901 ggcaggtcca agccctctct taggagattc acccccacaa gcacatcgaa ctcacctcgt
     6961 ctaaatgcag taagtatctc aatgcgctct atagttttaa tgtcgctatg taagtaactc
     7021 gctttgatct tcttctcctt caaatattcc gagagatcct ctgccattct cttggtaagg
     7081 gttgttgcta tagctctgcc acccttctct atcacttttt ctgcttcctc gatgaaatca
     7141 aaaatttgtc ctcgatagtc ccccctttca ttaataggtc taatggttac aactgggtca
     7201 atgaggccag tcgggcggat gacctgctca acaatttgcg ctgagttctc tctctcgaag
     7261 tcactaggag ttgctgaggt aaaaacgatt tgccccactc tctctttgaa ctcatccata
     7321 gtaagaggtc tgttatcttt agcactaggg agcctgaagc cgtgctcgac caaggtcttc
     7381 ttccttgctt ggtctccggc gtgcatgcca ccaatctgag gcaaggtaac atgtgattcg
     7441 tcgataatgg tgagaaagtc aggtgtgccg tctgccttgt gtgggaagta tgagagcaga
     7501 gtatccggtg cctccccagg accacggttc gagaagtgac gagaatagtt ctcaatacca
     7561 ctcgtataac caatctcctt aatcatggcg agatcataag tagtacggcg cttcagcctc
     7621 tctgcttcca aaatttttcc ttccttggca aaaactttta actgttcttc tagctcttgc
     7681 ttgatactaa gtagtgcgcg cttggcttga tcatcttgcg taatgaagtg ctttgctggg
     7741 aagatgaagt agaagttagg ttccgaaata atcgacatag ataccgcatc aatcctctta
     7801 atacttttaa tcatgcctcc atctatttct actcggtaga tcatcctttc gcctgtgggc
     7861 atgatctcaa tagcattgcc cagtgcgcgg aaggtgcccg gcttcacatc tgagttagta
     7921 cgagagaaat gaatacggat tagttcacgc acaaagtcag atctgctaat actttgaccc
     7981 acttcaatct ttatattcac cttctgatac tcttctggac ttccgaggcc atagatacag
     8041 gacactgagg caactatgat cacatccttc ctagttagta atgcttgagt agtagcgtga
     8101 cgaaggcgat ctatctcctc gttgatcatg gcctcctttt caatataagt atccgtgact
     8161 ggcatataag cctctggctg atagtagtcg tagtaagaga caaagtagtg cactgcattc
     8221 tcagggaaga actctctata ctcctgtgca agttgtgctg cgagagtctt gttgtgagcg
     8281 ataataagag ttggcttgcc aatattctgt ataacattcg ccgcagtaaa ggtcttgccg
     8341 gaacccgtaa caccaagaag ggtctgatac ttgtggcctt tctttactcc ttctgtaaga
     8401 gccttgatgg ccttaggttg gtccccggcc ggctcccact ctcgttttat cttaaatcct
     8461 gacattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgc gtttgactaa
     8581 gttgattatg aaatagagat tgtggataga tccgagcgta ccaccgagca tctcgtgagt
     8641 gcggaagaga tggcaaagat atgcacgtga ataattttta catacatagc acccacactc
     8701 tgagtctagt gtgtttgtat catctcggaa ttctgcattg cgaatattta ctttaccttg
     8761 atgtgtgtag agggtgcctc ccctgccatt acgagtcggt gccacacagt caaaaagatc
     8821 aacaccattt tctataccca taagcaaatc ctcaggctcg ccaataccaa gaaggtgtcg
     8881 aggcttttct tctggcaata tttcgttcac ccacttcacc gcagtttcca tatcttcttt
     8941 agcaaaggag cctccgatac caaaaccatc aaaatcgaga gagccaatga actctgctga
     9001 cttctttctc aaactttctt ccctacctcc ttggacaata ccgaagaggg cttgtgtctc
     9061 ggcatttggc ttcgacttgt gatactcgag acttttcttg gcccatctat gagttctctc
     9121 gagcgcctcc tcttgataag caaaagtttc agacggcgaa gtgcattcgt cgaaggcaaa
     9181 gattatgtct gcacctatgt gatgctgtat atctatggac ttctctggtg tgatgtagtg
     9241 tagcgatcca tcatgatgag agcgaaagga gactccatca gcaccaatct ttgccagact
     9301 tggtgtctca ccatcaagag atctttctgc aagcatttgc ctagggtctg taatacttac
     9361 caccttggaa agctccttgc catacgctgc tccaagagag aaaacttgga accctccaga
     9421 gtcggtcata gttggtccag accaattcat aaacttgtgc agtcctccaa agttcttcat
     9481 cctctctgga cccgggtcga ggtataagtg gtaagtgttc gcaagtacca cctctgctcc
     9541 aaggtccaga agttgctctg gagtaattgc cttcacggta cccttggtac ccactgatac
     9601 gaaagccgga gtattgatct tgccgtgagg ggtctctata actcccgcac gaccaagagc
     9661 accatttatc ttctttagta ttccaaaaga taatggggcc attatctttt ggtaataata
     9721 gatttcttat aaacatcaag gtgctcaagt gccacgcccg taccctttgc cacacagtag
     9781 agcgcttctt tggccagcac ggccttgacc ccagtacgcc tgagtactag ttcggggaag
     9841 ttcctaagtt gagatgatcc acctgtcatg atgatcccct tgtcgataat gtccgatgaa
     9901 agctcgggtg gagtattttg gaaaacatct ttaatagcac caaccatctc tcgaagctcc
     9961 ttagaaattg ctttagtaat ttcgttgctc tttagctcaa tagttcttgg tagtccgagt
    10021 atcgtgtccc tacccttcac ttgcatagaa agttcatctt cggcttggag gattacagca
    10081 ctaccgatct tgatcttgat ctcctcggcc gtcttgtctc cgatcgagag gttgtgcgtt
    10141 ctctttatat aatcctgtat agcagagtct actttgttgc cagcgcactt caccgaagtg
    10201 gatgcaacta tacccccaag agagatcacc gctacatctg tcgtaccacc ccctatgtct
    10261 actatcatgt gacccgcggc ttcttgtata ggtataccag cacctattgc agccaaaata
    10321 ggctccttga ccacatatgc attcttagct cctgccttaa gggttgcttc tatcaccgct
    10381 cttctttcag tggaggtgac gccagcaggt acagagacca agacatcggg tttaaatata
    10441 ttccacttac ctaaagcctt gttgatatag taacggagca tagcctccgt taccctgtag
    10501 tcagctataa caccgtcctt catcggtcta taggccacta tgtcctctgg agtcctacca
    10561 accatagtct tggcctcggc cccaatagct aggattttgt tgtctgatat agatacggcc
    10621 actaccgaag gttcgttcat aatgataccc ttcccgggca caaagactag aatattggtg
    10681 gttccgaggt ctataccgag cttcttctgg agactgatca tgggcacaat aatagctcct
    10741 ccgctataat gaggcaatga aggtcgtaga ggttgtaccg attgtcaaag gctttaccag
    10801 agagaccctt tcctacttca gcaaggagag cttcgcacca ggtagcttcg tcaaaattcc
    10861 acttagaaat ggatctattc ttggcatcgt agaaagcact gtcgacgtca gtcagaagaa
    10921 aagtttgatt aagaaagctg gcttcccact aaaaaagatt tctctagcgc ccaagcaagc
    10981 cttgtctaga ggctttatga aagctgttga gactacttcc aaattctatg ccactagctc
    11041 gggctcagta cttggtacac tacttaccaa gttttttctg gacaacccaa acttccttaa
    11101 gaaaaattcc agaactaact ctgagacaag cgaggttaaa ctaattcagc ttagtgaaga
    11161 ggaaagatac cgtgagtatc ggggtgtcat aagagagtct ttcgccaagg ggtcctctgt
    11221 catgtttatt gcacccacca aaaaagactt ggacaaagca aagaacagcc ttaatgttgg
    11281 tatcgaaaaa tttgtgtact cgactgccgg cattactccc aaggggttaa agaagctttt
    11341 cttgaaaatt catgaggaga agcacccggt tcttataatc accagcatga gtaattgtgt
    11401 cttcgataga cctgatttgg atttaataat agtagagaga gaaaactcta gagcctacag
    11461 aggactcaag cgcccttacc tagactataa aatgtttctc aaaaattaca ctaaggaatg
    11521 tggtaaaagc ttaatattcg gcgactcggt tctaagtctt gaaactcttt ggctcgaaaa
    11581 agaaggcatg tatagtgccg tcactcctct tacttggaga ctcaaggata aagcttcagg
    11641 ggagatcatt gacatgagag agaagaaaga gttcgaaatc atttctgaga aacttcaaaa
    11701 aaggattaaa atgtctctaa cagagaacaa aaagatcttc ctcttcggag tgcgcaaggg
    11761 cctctcatct acaaccgctt gtggtgattg tggctcgctc ctactatgca agaattgttc
    11821 atcacccctc gtactccata cccaacagat tcaaggcgag acacttaact actacaaatg
    11881 tcatcactgt ggcgccaaga ggactgcaga gactcgctgt gactcatgcc aaagttggaa
    11941 gctcacgccc ctcggtatag gcatagacac tattgctaaa gaatgtagag agaagatgcc
    12001 tgaggtcaat gtctttatcc tgagcaaaga gcacacccca accaagtcca aagctgtaga
    12061 aactgttaaa cgcttcttcg atactccgca aagtattctt ataggtaccg aaatggcaat
    12121 agagtaccta gaaagcgtac ccactgttgg agtggtctcg ctcgactcgc tcttctccat
    12181 acctaacttc tatataaatg agaggatctt ctacctagtt actagactta aggagatcac
    12241 cggagagagt tttctcatcc agacgagaaa tgcgggtgta aacatcctcg agtgggccac
    12301 agcaggcaac atcctcgact tttaccgaag tgagatagaa gaaagggagg atttgcatta
    12361 cccaccattc tcttacttca tcaaaatcag tttcgaagga acaatagata aggttgaagc
    12421 caagtcgatc ttactcaaga acgattttga gctcttcaac ccacacttta tgatcaaaaa
    12481 gagtggccaa ggaaaaacag ctgagctaac aatgattatc agaatttcta gagagaactg
    12541 gccagacgaa tccctacaga ggaaactcct tttgctaaca cccgactttt tgattaaagt
    12601 agatcctgag agcatcttct aatatgccca aaacaaacat cttacaaaaa gactccccca
    12661 tccttagaaa ggtagcctta gatgtgccag aaaatgagat ctcatctcaa aggatcaaaa
    12721 cgataataag caagatgaag atagcggtac atgccgagga tgacggtgta gctattgcag
    12781 cacctcagat aggtgtaagt ctacgaattt ttgttgtagc tggaagaacc ttcaacattg
    12841 gtaagaaagc ggacaaggaa accgttttcc ctgacctcgt ctttatcaat ccagagatca
    12901 ccaaactctc gaaaaagaaa aaaattatgg aggagggttg tctctcggtt agatggctct
    12961 atggaaaggt cgaaagacac gaaagagtct ctattaaggc tctcgacgaa acaggcagaa
    13021 aaattgagag aggtgcgtca ggactcttgg cacaaatatt ccaacacgag atagatcacc
    13081 tcgacggcac tctctttata gacaaagcaa cagatatcga ggatgtacca ccaacacatc
    13141 aagatgaata aactagactt tatctttttt ggttcttcta aattctctga gtatgttttg
    13201 aatgtgctca gagataaagg ttacgagcct aaactttgta ttacgagcgc caagggcgat
    13261 ctaccagaaa tacctgatgc cgatctatat atagttgctt ccttcggtaa gattcttaag
    13321 gatgaactta taaacaaacc taagtataag actttaaatg tccacccatc tctactaccc
    13381 aagctccgcg gacccgcacc tatccagggt gcaatactta gtggtgagaa aaccggagta
    13441 agtatcatga gaatgaatgc caaaatggat caaggcccag tgctagctac aaaagaagtt
    13501 attttcgaaa acaatccaga gcactatgtc atagctgagg aaatacttgg cacaattgga
    13561 ggggaactct tggcagaagt gatacctgga tggttatctg gcgagattaa ggagaaacca
    13621 caagacgatt ctcaggcgac atacaccaag cttattaaaa aagaggattc agatattacg
    13681 aatgactcac cagaagtagc aatgagaaag atacgagcat acgaagtttg gccacgagca
    13741 agacttggcg agctcatcgt tactgacgct cacatagaag gaggaaaact agtgttagac
    13801 aaggttatac caccaggcaa aaaagagatg gtttacaccg attacctacg aggtaagcat
    13861 taattacctt ctccacttga tccacgggaa tccttgaatc atatttttta tagctctctg
    13921 accgctccta agtaactctc tcttcttgct ggtttctcgg gtgacatcct gtataacttc
    13981 tgtctctaca agatctattg ccctatacaa gtcatccgcc tcctgggcaa catagttatc
    14041 catccctgcg cccgagagcc tggcctcagc cctaaatata tctccatgct tgtggtggtc
    14101 agtggtcttg ccaagttcta tagatagtaa gacatccttg ttaccaaggt acttctccag
    14161 ttttgaaaat ctttttaatg cataatctct tatagcctcg gttaactcaa tattggttgc
    14221 tttgatattg gttttcatag ctctattata tcacttgctt tgatcgtgtg ggtcaaagat
    14281 tgatggggca acttcatcat tatttaagaa ttttgatatt ttctttccac caaaaattga
    14341 tggtagtatc cccaccccat tacatcctag gttataaagc aaaactgggt taagaggttc
    14401 tggcccaatc cttcttattc cattaggagt gtaacccata agtccatgcc agcaaaaagc
    14461 gtactctgtt tccttttgat gtttaccata gtttgctaag aggaagtcgt ttatcttatc
    14521 ttgcacatcc tcgtcgcatg catgtgccct cgagtacaag gcgccattag gcaacacctt
    14581 ttcaggtcct cctgcacaaa taaggttgaa gtctctgtcc ttctcaccct cattctcatg
    14641 aggcctcctc gtaagataaa agtacgtgtc tcctgttgga tcgttcgaat ttatctcgct
    14701 ctttgagaag taactaatag ctgtcggagg gtggtttagt ggctggatgt agcccgccat
    14761 ataaccaatg cgtcctgcca cagagtgatg aaactcggta ttaatgtctt gccccacatt
    14821 attatcaata gaaaaattct caaacccgtt tgtgcagagt actactcttt tagtttgaat
    14881 attcttgttt aaaacttcaa gcacagcgct ctctttatcc aaacgaacaa ttttcaaagg
    14941 actctcttcg aagaaggaga atctatcttt ataagttgct gacaaataac ccacaagctc
    15001 ttcggtaagc agagcgctgt tagtacaacc cttctggtat gagatgcagg ctatgtagtc
    15061 agaattattg gtgtctagta gatcaagaat atctttgtgt ttggcaacgg agtaatactc
    15121 tttgtactca tcactgatat cttttaatcc atcccaactt tcagaaatta ttattgactc
    15181 tctttgcaaa ccatttcttt ctctgtattt atttgattct aaaaaatcta caagcttgct
    15241 catacctgcc acccctgcgt aaccagtaaa gcgatatatt ggggtcctga gctttgcttc
    15301 ttctaccatc tcgtcgagga gcagccatgc tgactccaca tcccttactc cgtctgtagc
    15361 taacttttca ccaaattcct ctaccatacc tggaagcggt ctctcaaaat aactcgcgag
    15421 ttgcccagcg ttatgcccag ttgcgccgtg tgccaccttg tctgcttcta tgagcactac
    15481 tctatgcttt gtatctttaa gtgcaaagta tgctgtgaca acacccgcaa taccaccacc
    15541 cactatggtt acatcagtct cgatatcttt atccaaggga acagctggcc tagagcgatt
    15601 aagctgcttt atccaagggg aggtgttcgg aatcatttac caatattcta gcttattcga
    15661 ccctactttc aaaaaggttt agaagataag catcgatctg cctnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnntgctcgg agtaaccctg atcagcagag aacttgttgg
    15841 ctgtgcccgc accataagtc accgtatatg cgttcttcaa acttgtttgt gactcgaatg
    15901 acctgtaacc cgaaagaatc tttaaatcca taccgtctat atctgcctcc tctaatagtc
    15961 tctgtaggaa tgggtacaca agagcaagga tttgctgtgg cttggcgggg ttactgagga
    16021 aaaccgaact aatgtctgta agacctctgg gaacataatt ctcattcaaa aagtagacct
    16081 tggagtattg cttcaagagc tctctatctg tctcggctaa cttcttcaac ttctccaagg
    16141 tgctagagag tccgtcgatc tgtgaactgt attcgtctat gtcaccacga gccaatttta
    16201 gatcgtcagt caacttcaaa ttttcttcta gagtcttgcc aagagtctct ctagtttctg
    16261 taagcgatgt gttcaaatcc aaattttttg aataaaggtt ccataagcca aatactgcta
    16321 tcaacagtaa ggctattagt ccaatcaaat agctcttatg agttttatca agtgacatca
    16381 gacaattata tatgaaaaag aaccaaatcg aatttggttc ttctgagtgg ttcacttaac
    16441 ccacatcaaa ctgtggtgac gagcttaccg agtagacctc ttccacttct cgagctgctt
    16501 gacagcctgc ttgaaagtga ggtcgccttc acggtaggcg ttcaaaatag aaccaggatt
    16561 atcagtgaaa acgatacgca ggcgcttagg cttcctctga agcgtattgt gaagcgtatt
    16621 gactgcctca gtgagtccac tccagaatgc ggactcttga atctggcgta ctgactccat
    16681 gggtagcctc gtaattcagg tttgtattgg cgaacagtct ctataacaaa aaataacatt
    16741 tatacttgat atgtcaatac tacattttga taacagaatt cttgacctat agacactcaa
    16801 aatgtaggat tgctgtgggt gtgagggact aggaagaaag caaccgcgat tccagaaatg
    16861 gaacagccta aacctcaagg aagatccaag gtacttgagg ggtcgaaggg tacaccacgc
    16921 cttgaagttc gcctggttat gccctaagta gtggattggc tcgccaaacc acgaatcagc
    16981 gagtactcaa tcgcacctcg caaggctaaa ccgaccccct ctcacccacc cccgcaagtc
    17041 ttacagcttg cggctttttt attccttgga agaaaactcg attatccatt caataccgta
    17101 tttgtctcga aacattcctg catatgtacc ccaagggctg ttgcccattt gtccttcgac
    17161 ctcccctcca agagaaagag tcttaaataa tttttctgct tcttccttac tttcggcgct
    17221 taccacaatt ttactcctgt tttcacgttc atttatcttg cccataaact ctggaacatc
    17281 attcgccatt aagatacttc cgccaccaat aggtagggca atatacataa ttttgttctc
    17341 ctcgttttct ggaactttaa attcttcact tgctagatct ttgaaacgaa ctatttttgt
    17401 aaactctcca ccaaatgctg atttgtagaa agtaaacgct tcctcggcat ttccgttaaa
    17461 attaatccaa ggatttattg ttgtcat
//
LOCUS       DAORIT010000012        17050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_564_length_17050_cov_47.110385, whole genome shotgun sequence.
ACCESSION   DAORIT010000012 DAORIT010000000
VERSION     DAORIT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17050
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_564_length_17050_cov_47.110385"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..927
                     /locus_tag="PLD99_01630"
     CDS             214..927
                     /locus_tag="PLD99_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRH25638.1"
                     /translation="MPEEQNTHNHTQEEKDRIKKIRMERIHSEFEEGFKFIDEYPKSV
                     TFFGSSLLKEDDPYCISARTLAGRIAKELNYAVLTGGGPGIMEAANRGAHENGGSSLA
                     LTIRLPHPQIVNPYITRMVDPNYFFVRKVLLSFNAAAFIFFPGGYGTLDEFFEILTLV
                     QTQKVVGVPIICVGTDFWSSLKVFIEKELLTRGTILPDNVDLFKITDNHDEILEIIKK
                     TPIRDNLDIHEVKQVSEIS"
     gene            complement(924..1355)
                     /locus_tag="PLD99_01635"
     CDS             complement(924..1355)
                     /locus_tag="PLD99_01635"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HRH25639.1"
                     /translation="MKKVLYILSILLVVGIAWFYSRGVDSQIDLNQAGSLATQDEVVV
                     GYPKSGDLFRSGDKVAGRARGPWYFEASAPVKVAGPNGESLGGGYVTAQGEWMTTDFV
                     SFEGTINFDKGRNKSGYLIFMNDNPSGEESRLKYFVVPVKF"
     gene            1374..2117
                     /locus_tag="PLD99_01640"
     CDS             1374..2117
                     /locus_tag="PLD99_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25640.1"
                     /translation="MQKKIIIVVIFIVLAFLGYRSFVMAPEVVTSTESSSTSTSTPTS
                     TPATKPTPSKNDLLIKDAAWKVFEAYLKATKDKDIETVKKLSYQVSSDCLDQTKVKEC
                     QDRMNTAFIYGKQFKYENMKYISYDEKQIILAGDYFESLEGESPSFARGVLYFIREGS
                     TVKFLSLNPFFGAIIIREEGEATTTVRDRLLNATEDTDVDYALDVFEKCIGIGALPGC
                     VKTDSTKRDTNGDGFWDSTESLFYKQSTQ"
     gene            complement(2107..3276)
                     /locus_tag="PLD99_01645"
     CDS             complement(2107..3276)
                     /locus_tag="PLD99_01645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRH25641.1"
                     /translation="MEYRRRGFTPHKRFSNSRPKQQKFFSDVSKFINKAVITEEVEVF
                     TPEHMFKDFAINDKLKANIEEKGYIAPTPIQDRVIPHILRGEDVVGIANTGTGKTAAF
                     LIPLIDKVMKNENEKVLIVVPTRELAFQIDEELRAFAKGSGLFSVICIGGAGIGKQIT
                     ELRHKHSFVIGTPGRLKDHIEARRLNLALFNNVVLDEADRMLDMGFINDMRYMMERTP
                     KTRQTLFFSATLSREIEALIGEFLNNPAKVSVKTRDTSKNIDQDVIRIQGKTKAEVLL
                     DLLVQEDFDKVLVFTRTKHGADKLSKFLSTSGVKTEAIHGDKQQSARLKALKEFKEDR
                     VKVLVATDVAARGLDISDVSHVINFDLPATYEDYVHRIGRTGRANKKGKALTFIE"
     gene            complement(3400..3789)
                     /locus_tag="PLD99_01650"
     CDS             complement(3400..3789)
                     /locus_tag="PLD99_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25642.1"
                     /translation="MNKKLILAIIVLGALVYVLSQGGDRVTSGIKGVVRLGPTCPMQK
                     NEDDPNCADKMYQTKLELTRFNSVEVLKTFSSNKDGEFEVSVAPGEYSIREAGLGKEG
                     KCGKNGLVPVEANQFTEVIVFCDTGIR"
     gene            complement(3782..4321)
                     /locus_tag="PLD99_01655"
     CDS             complement(3782..4321)
                     /locus_tag="PLD99_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25643.1"
                     /translation="MRRRLIILLGILIVTIATSLLWYTGKIEKYNSRNFGMAFYYSNK
                     YFLEEKVVSDKEKIVILTEDTEENRLVREGKSPGREGPTAITLRVIKLSNSSTTPLEW
                     AKNNMESNFHLAIGEHHEGKVGKRDSVYYITDGLYVADNIVVLANGRMVHISVTYLTP
                     EDKIRKDLARIIGSIYFYE"
     gene            complement(4318..4941)
                     /locus_tag="PLD99_01660"
     CDS             complement(4318..4941)
                     /locus_tag="PLD99_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRH25644.1"
                     /translation="MFQSISHKYIKFGLVVLLIIGIFFLLRDERTITNYPSQGSDIIA
                     FGDSLVAGVGSSTGGFVDMLSKDLGVEVVNLGISGNTTKDALDRVEVLKDYKPKLVMI
                     LLGGNDFLRKVPTEETFANLDKIVRVVVNQGSVVVLLGVRSGVLSNRYDEEYEKLASK
                     YGAVYVSDVLDGVFGRPNLMYDTIHPNDLGYRIIADRLNPVIKKLIQ"
     gene            complement(4919..5254)
                     /locus_tag="PLD99_01665"
     CDS             complement(4919..5254)
                     /locus_tag="PLD99_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HRH25645.1"
                     /translation="MAKTVKKTVKKAAKKVVKKAAKKPAKKARKANAAFMKPMKISND
                     LGMVIGKGPMPRTEVTKKLWAYIKKNKLQDEKNKRNINSDEKLKVIFGGKKTVSMFDM
                     TKLVSKHLS"
     assembly_gap    5276..5375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5470..6159)
                     /gene="rnc"
                     /locus_tag="PLD99_01670"
     CDS             complement(5470..6159)
                     /gene="rnc"
                     /locus_tag="PLD99_01670"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRH25646.1"
                     /translation="MDFSKFEETLGLSFNNKALLRQAFTHRSYINENRSSGLTHNERL
                     EYLGDAVLQLVTTDFLFRKYPDRDEGELTSIRSALVNADTCAEVAQKLSMNDFLLLSK
                     GESKDTGRARQYILANTFEAIIGAIYLDRGFAEAINFINKHITPLTEAILEGGKWVDA
                     KSLFQAKAQENLSHTPMYKTLSESGPDHDKQFSVGVFVGEEKMGVGEGKSKQDAEQAA
                     ARDALKKQGWQ"
     gene            complement(6168..7085)
                     /gene="nusB"
                     /locus_tag="PLD99_01675"
     CDS             complement(6168..7085)
                     /gene="nusB"
                     /locus_tag="PLD99_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRH25647.1"
                     /translation="MSHRHLSRSVVLQTLFEWDFDISNGKSVDAYEVFGRQVQEFAPD
                     SKDRPFMNNLLKNVVDKKNDLDNIIVKAAPDWPIEKISIVDRNILRMGLAELLFADRG
                     EVPPKVAINEAIELAKSYGGETSGRFVNGVLGAVYREMGEPGKDDQPKPKKKYGNVPD
                     SELPLEKLAGAVVYAKENGETYLALVHDIFGHWTLTKGKLEGEETEEAALVREAEEEL
                     GVPIVVKEKLGENAYTTFHPEKGKIKKQVTYFLAEAPYQELTLEKKEEKGGLDDVRWF
                     RLADILGLNFYDDILPIVTKALQILAQTG"
     gene            complement(7106..7252)
                     /gene="rpmF"
                     /locus_tag="PLD99_01680"
     CDS             complement(7106..7252)
                     /gene="rpmF"
                     /locus_tag="PLD99_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRH25648.1"
                     /translation="MRHTRAHTKNRRSHHALENPALTVAKSGAVHLRHRAHPKTGEYR
                     GRKV"
     gene            complement(7344..7862)
                     /locus_tag="PLD99_01685"
     CDS             complement(7344..7862)
                     /locus_tag="PLD99_01685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRH25649.1"
                     /translation="MTATMRQMKTPQDLGYINPSEDDSHLGVAVICRDGDEEREYLVM
                     EYDSGDGVQIKFPAGTNNDRPGELIFDTCLRETEEETDLILPNPSECVWVGPRQKNRD
                     KPGFHQKVAFISRFEDCGGQLRTEEKKDDDGDKHSPPFWRTESELLVQVEDGGLFWSH
                     RPILHAAKMHQF"
     gene            complement(7943..8530)
                     /locus_tag="PLD99_01690"
     CDS             complement(7943..8530)
                     /locus_tag="PLD99_01690"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRH25650.1"
                     /translation="MTDIIGIDEAGRGPLAGPVAVGVAKICHDFDMSFFEGIKDSKKL
                     TEKGRKEWFAKAEEMKREGKLNFTVELVGADVIDKRGIVYAIKLGMSKCMKRLGVDED
                     AKIFLDGSLHAPKEFKNQETIIKGDEKIALISLASICAKVARDQYMTKLATEYPEYKF
                     DIHKGYGTKAHIEAIKKHGSSAVHRMSFLKNILKA"
     gene            8554..11709
                     /gene="dnaE"
                     /locus_tag="PLD99_01695"
     CDS             8554..11709
                     /gene="dnaE"
                     /locus_tag="PLD99_01695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRH25651.1"
                     /translation="MSKFVHLHTHSHYSLLEALPKISDLVAHAKKHEMKALALTDAGN
                     LYGAIEFYKECKDEGLKPIIGVDAYIAPRTRLDKEARIDASRSRLVLLAENIVGYKNL
                     LKLVSLSYLEGFYYKPRMDKEILEKYKDGLIAILPSFNNTELEEQITWYKKTFGDKFF
                     VEITHHPEIANHEDKQSERIALSKKLGVEIVAAHDVYYLEKADRPARETLMSIQSHIG
                     VGRGRSFEDEEDDFSFISPKEAEKRFKSTPEAIDNIEKIVDMCNVELKLGDWVFPNLE
                     IPDGKTPDEALREIVEAGLIRRKLEKTKEVEERIEYELKVISDKGYPKYFLVVSDLLH
                     FAHENGILTNIRGSVAGSLVTYLAGITNVNPLEYKLPFERFLNPDRPSAPDIDMDFAD
                     DRRDEVIDYARKKYGEDRVAQIGTFGTMMARGAVRDVTRAMGFAVAIGDKIAKLIPMG
                     AQGFPMTIDRAKKESPDFKKLYTSDPDVKRIVDMAEKIEGNARHISVHAAGVVISPSP
                     LTDYTALQYDTKGENKVITQYDMYSIEEAGLLKFDFLGIRNLSIIADAINRIKKIEGV
                     EIDMDEIPVDDKKTYEMLARGETEGTFQLNGDGMTKALMELKPTVIHDINVMVALYRP
                     GPMDNIQEYIARKHKKKPVTFLHPKMKDFLDTTYGVLVYQDDLLMTAIEVAGYSWGEV
                     DKFRKAVGKKIPEEMKKQHVMFVDGCQKHGGMSKDKAEALWELFVPFQGYGFNKAHAA
                     SYGHIAYITAYLKANYPAIYMSAVLTAESGDVETIGVMVKECKRMGIEVLPPSVNESY
                     SQFTVPPRESKDDKYKIRFGLTTIKNFGQGVATSIIEERKKGGKFKSLIDFLDRVKDK
                     NLNKKSLESLIKAGALDDFAERGEMLQNLETLLAYHKESSAKATNQDSLFSGLTGSSA
                     LPPLRMTKAEPINKKERLMWEKELLGLYITGHPLDEFSEKLDKAKNNSLNLKDAPENA
                     ECVIGGVITDLRTTLTKAGEMMAFARLEDLKGSVDMVVFPKVYEEYKEFLQGDQCIAV
                     KGRIGKKNDELSFIVEKVKSL"
     gene            complement(11737..12195)
                     /locus_tag="PLD99_01700"
     CDS             complement(11737..12195)
                     /locus_tag="PLD99_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25652.1"
                     /translation="MSKLSSFMARKEMFDRMKKEMELGAGLVVEFAKKNLSRLKWNSR
                     VGVVSRFTFFDGKCEWQLAHFGGRTEVVLIAHVPSTNDSESLEERESRFDYPIMGAST
                     QMAVRHSALVHIETIRLSFDEFLDHFAVVHPEFQKFIEPFDQCRPTCQEG"
     gene            complement(12329..12730)
                     /locus_tag="PLD99_01705"
     CDS             complement(12329..12730)
                     /locus_tag="PLD99_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25653.1"
                     /translation="MKAVDFSAVITTVLRESNLKTVEEIEPLLDAFLQWFSLVPEALD
                     GKPLQMLESVDPIWHAMILNTAFYRRFCEEFIGEFVDHNPMDVTQNTRAVKRAYADHT
                     LALLRRTYGDSVNPALLSLHDSITCCCGGSC"
     gene            12945..14162
                     /gene="thrS"
                     /locus_tag="PLD99_01710"
     CDS             12945..14162
                     /gene="thrS"
                     /locus_tag="PLD99_01710"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRH25654.1"
                     /translation="MMNVEDNDHRKIGKELDLFTFSDLVGSGLPLWTPKGTVVRGELD
                     KFVWELRSKHDYSKVTIPHITKRDLYDTSGHWEKFSDDLFKINSREGKEYALKPMNCP
                     HHTQIFARKPHSYREMPQRYAETTMVYRDEQSGELGGLTRVLSITQDDSHVFCRTTQI
                     KEEFNKIWDIVDNFYKTFGFSIRVRLSFRDPSAPEKYLGTKEIWDKAESELREIATER
                     GADYFEGPGEAALYGPKLDFVAKNAAGREWQIATIQLDMNLPERFDLSCVNEKGEKER
                     IVMIHSAITGSIERCVAIILEHLEGVLPLWLSPVQVKVLPIGESHFDYAKSVFESLKS
                     ESIRAELDESNESLGKKIRNAKTDKVFYTLVIGDKEVEDKTVTVEGREEKLGAMSVEA
                     LISKIKDEIKNRK"
     gene            complement(14826..16880)
                     /locus_tag="PLD99_01715"
     CDS             complement(14826..16880)
                     /locus_tag="PLD99_01715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HRH25655.1"
                     /translation="MAKEPKKTSNYSAESITVLEGLEPVRKRPGMYIGTTGPDGLHHL
                     IWEIFDNSRDEAMGGFANDIEVSLLPGNYIRVVDNGRGIPVDVHKQTKVSALETIMTT
                     LHAGGKFGGEGYKVSGGLHGVGASVVNALSTHTIAQVHKDGGVYLQEYNAGKRKAAVK
                     KTGGTKNQGTVITFRPDPTIFPDTNFDFKRVVNHLRQQAYLVKGLRITVIDAREEAMV
                     ESEDAFYIRDLALNVPSMSFYFEGGLMSLVRFENELQKPIHKNIFYVEKAANGNDYET
                     VEVALQYVDDISSRILPFANNIYNSEGGTHVTGFKTALTRTLNTYARKNNLVKESEDN
                     FTGEDVLEGLTVVISVKLREIQFEGQTKGKLGSVEAQAMVTTIFGEAFSTFLEENPDD
                     ARAMINKVVLALKARKAAKAAKDSVLRKGALEGMTLPGKLADCQSKSAEESELFIVEG
                     DSAGGTAKMGRDRRTQAILPLRGKILNIERARLDRMLGSEQIKNLVVALGTAIGDTFD
                     LEKLRYHKVIIATDADVDGAHIRTLLLTLFYRHFRQVIDGGFIYIAQPPLYKIKKGKE
                     VHYAYTDEEKNKIVGKDINVEDVIEENAEIKEEEEAEEETTKKVKSNKVSIQRYKGLG
                     EMNAEELWETTMDPKVRILKQVTVEDGRDADKVFDILMGTDVPSRKSFIQSNAKKATL
                     DV"
BASE COUNT         4584 a         4104 c         3648 g         4614 t
ORIGIN      
        1 tgtcctgtac cagctaccaa atcatccttt tcgtatccag ttgtaatgtt tgtactcatg
       61 gtgttgttct cttttggtgt gttattaaat aaattcataa agaggttacc ataaaatagg
      121 aaccccaata ctactaacgc tgcagctacc cctataacct tgtttactgt tttgttcata
      181 attttacttt aatgtcatga tattatagcc cttatgccag aagaacaaaa tacccacaat
      241 cacacacaag aagagaaaga tagaataaag aagataagaa tggagaggat ccatagcgag
      301 ttcgaagagg gattcaagtt tatcgatgag tatccaaagt cagtaacatt cttcggatca
      361 agcttgctca aggaagacga cccttactgc atctccgctc gcactctagc tggtcgtata
      421 gccaaggagc taaactacgc agtacttact ggcggtggtc ctggcatcat ggaggctgcc
      481 aaccgcggcg cacatgagaa cggcggctct tctctcgcac tcaccattcg cctaccacat
      541 cctcagattg ttaacccata catcactcgt atggttgacc cgaattattt cttcgttaga
      601 aaggttctcc tttcatttaa cgcagcagct tttatcttct ttccaggagg atacggaaca
      661 ctggatgagt tcttcgaaat acttactctc gtccagactc aaaaagttgt tggcgtacca
      721 atcatttgcg ttggtactga cttttggtcg tctctcaaag tgtttataga aaaggagctc
      781 ctaaccagag gtaccatact ccccgacaat gttgatctct tcaagattac cgacaaccac
      841 gacgaaatac ttgagatcat caagaaaact ccgatcagag ataacttaga tatacacgag
      901 gtaaagcaag ttagcgagat ttcttaaaac ttaactggta caacaaagta ctttaaccga
      961 ctctcctctc cggatggatt gtcgttcata aatattaagt aaccactctt gttccttccc
     1021 ttgtcaaagt taatggtgcc ctcgaatgac acaaaatcgg ttgtcatcca ttcaccctga
     1081 gccgtaacat aaccacctcc caaactctca ccgtttggac ccgcaacctt aacgggtgca
     1141 gatgcttcaa agtaccaagg cccacgagcg cgaccagcaa ccttgtcccc acttctaaac
     1201 aagtcaccag actttggata ccccactacc acctcatctt gagtagccag actacctgct
     1261 tggtttagat ctatttgact atctactccc ctcgaataaa accaggcaat acctactacc
     1321 agcaaaatgc tcaagatgta taaaactttc ttcatactgc tataataccc cacatgcaaa
     1381 agaaaataat aatcgttgtg attttcattg tcctagcctt tcttggatat agatccttcg
     1441 taatggcacc agaagtagta acatcaacag agagttcatc aacaagtacc agcactccta
     1501 caagcacacc tgcgaccaag cccaccccaa gcaagaatga tttattgata aaggatgctg
     1561 catggaaagt tttcgaagct tacctaaaag caactaagga caaagacata gaaactgtta
     1621 agaagttgtc ttaccaagta tctagtgatt gtcttgatca aaccaaggtt aaggagtgcc
     1681 aagatagaat gaacacagca ttcatctatg gaaaacaatt taagtatgaa aacatgaagt
     1741 acatctctta tgatgaaaag cagatcatct tggcaggaga ttactttgag tcacttgaag
     1801 gcgagtcacc atctttcgct agaggagtac tatactttat tagagagggt tcaacagtga
     1861 agttcctatc actgaaccca tttttcggag caattattat tcgagaggag ggtgaggcta
     1921 ccacgacagt aagagacaga ttactcaacg ctacagaaga tactgatgta gattacgccc
     1981 tagatgtgtt cgaaaagtgt attggtatcg gcgccttacc tggttgtgtt aaaacagata
     2041 gtacaaaaag agataccaac ggagacggct tctgggactc taccgaatcc ctattctaca
     2101 aacagtctac tcaataaagg ttagtgcctt gcccttcttg ttggcgcgac cagtgcgtcc
     2161 gatacggtgg acatagtctt cgtaagtagc aggtagatcg aagttgatca catgtgagac
     2221 atcgctgatg tcgagtccac gagctgccac gtctgttgcc acaagcacct tgaccctgtc
     2281 ttctttgaac tccttaagcg ccttcagtct tgctgactgt tgcttgtcgc cgtgtatcgc
     2341 ctcagtctta accccagagg tagagaggaa cttagaaagt ttatctgcac catgcttagt
     2401 acgggtaaag acaagtacct tatcaaagtc ctcttgaacc aaaagatcaa gcagaacctc
     2461 ggccttggtc ttgccttgta tgcggataac atcttgatca atatttttag aagtatctct
     2521 agtcttcacc gagaccttgg cagggttatt caaaaattcg ccaatcagtg cctcgatctc
     2581 gcgagatagt gtggcggaga agaagagtgt ttgcctcgtc ttaggggtcc tctccatcat
     2641 gtacctcatg tcgttgatga accccatatc aagcattcta tctgcctcgt cgagtaccac
     2701 attgttgaat agtgcaagat tcagtctcct tgcttctata tggtccttga ggcgaccagg
     2761 tgtaccaata acaaagctgt gcttgtgtct gagctcggta atctgcttgc caatacctgc
     2821 gcctccaata caaataactg agaaaagacc cgagccctta gcaaaggctc ttagctcctc
     2881 gtcgatctga aaggcgagct cgcgagtagg aaccacaata agcaccttct cattctcatt
     2941 cttcatcacc ttatcaataa gaggtatgag gaaagcagcg gtcttgccag taccggtgtt
     3001 cgcgataccc acaacatcct caccacgaag aatgtgtgga atcacacggt cctggatagg
     3061 agttggagcg atgtagccct tctcctcgat attggccttc aatttgtcgt tgatagcgaa
     3121 gtccttaaac atgtgctcag gggtaaagac ctcgacctct tcggtgataa cagctttgtt
     3181 gatgaacttc gaaacatcag aaaaaaactt ctgttgcttt ggacgactat tactaaatct
     3241 tttgtgcgga gtgaacccgc gtcttctgta ttccatatga cataaacctt gctctgaggt
     3301 ttatggcatt tagccgacga atgagattaa tccccagacg aaacctatga gtgttagtta
     3361 cttattcaat atatatcgtt ccatcaattt tgtcaagctc taacgaatac cggtgtcgca
     3421 gaagactatc acctcggtaa actgattcgc ttcgactggt actagaccat ttttgccaca
     3481 cttaccctcc ttgcctaaac ccgcttcacg aattgagtac tctcctggag ctacgctgac
     3541 ctcgaactcg ccatccttgt ttgatgagaa agtttttaaa acctcaactg agttgaagcg
     3601 agttagctcg agcttggtct gatacatttt gtcggcacaa tttggatcat cctcgttctt
     3661 ctgcatagga caagttggtc cgagtctaac aacacccttg ataccactag tcactctatc
     3721 ccctccctgt gacaagacat atacaagcgc accgagaact ataatcgcta gtattaactt
     3781 tttattcata gaagtatatt gatccgataa tcctcgccaa gtcttttctt attttatcct
     3841 cgggggttaa gtatgttaca gatatatgga ccattctacc attagccaag acaacaatat
     3901 tgtctgccac ataaagcccg tccgttatgt agtataccga atccctttta cccaccttac
     3961 cctcgtggtg ctcgccaata gcgagatgga agttcgattc catattattc ttcgcccact
     4021 cgagcggagt agtagaggaa ttagagagct tgataactct aagtgtaata gctgtcggac
     4081 cctctcgccc cggtgattta ccctctcgta ctagcctatt ttcttcggtg tcctcggtaa
     4141 gtatcacaat cttctctttg tcactcacta ccttctcctc caagaagtat ttgttggagt
     4201 agtagaaggc catgccaaaa ttcctagaat tatacttctc aatcttaccg gtataccaaa
     4261 gcagtgatgt tgctatggtg actataagta tccccagaag aataattaat ctccttctca
     4321 ttgtattaat ttctttatta ctgggttcag tctgtcggca attattctat aaccaagatc
     4381 attcggatgt atcgtatcgt acataagatt aggacggcca aaaactccat ctaatacatc
     4441 actcacatat acagctccgt acttgctagc caacttttca tactcttcgt cataacggtt
     4501 gcttagtacc ccgcttcgta caccaagaag tactactaca gaaccctgat tcactacaac
     4561 tcgcacaatt ttatcaaggt tagcaaaagt ttcttcagtt ggcacctttc ttaagaagtc
     4621 gttgccaccg aggaggatca tcacaagctt gggtttgtaa tccttgagca cttcaactct
     4681 atctagtgca tccttagtcg tgttaccact tatacctagg ttgaccacct caacacccaa
     4741 atccttcgac aacatatcga caaacccacc agtactcgag cctactccag ctacaagact
     4801 atcgccgaaa gcaatgatgt cagacccttg agacggatag ttagttattg ttctctcatc
     4861 tctaagcaag aagaaaatgc cgataatgag cagaacaact aacccaaact ttatatattt
     4921 atgagagatg ctttgaaaca agtttggtca tgtcgaacat tgagacagtc ttcttacctc
     4981 cgaagatcac cttgagcttt tcgtctgaat tgatgttcct cttgttcttc tcatcctgga
     5041 gcttgttctt cttgatgtat gcccagagtt tcttagtaac ttctgtgcga ggcataggac
     5101 ccttgcctat taccatgcca agatcattac taatcttcat tggcttcatg aatgcggcat
     5161 tcgccttcct tgctttcttt gctggtttct ttgctgcctt cttaactact ttctttgctg
     5221 ccttcttaac tgttttttta actgtctttg ccattctaat ttattaaatt tctatnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccttc ttaactgttt ttttaactgt
     5401 ctttgccatt ctaatttatt aaatttctat actcctaatt ataccccact ttcataggag
     5461 aaatctcagt tattgccacc cctgtttctt gagtgcgtcc ctggccgctg cctgctcggc
     5521 gtcttgtttg ctcttaccct cacctacccc cattttctcc tcaccaacga atacaccaac
     5581 agagaactgc ttgtcatgat ctgggcccga ctctgagaga gtcttgtaca ttggggtgtg
     5641 actcaagttc tcttgagcct tggcttggaa gagactcttc gcatcaaccc acttccctcc
     5701 ctcaagaata gcctcagtaa gaggagtaat gtgtttgttt ataaagttaa tggcttcggc
     5761 aaaaccacgg tctagatata tcgcacctat aatagcttcg aaggtgttcg ccaagatgta
     5821 ttgtctagcg cgaccagtat ccttggactc ccccttagac aaaagtaaaa agtcgttcat
     5881 agagagcttc tgcgccacct cggcacaggt gtctgcgtta acaagcgcgg agcggataga
     5941 ggtgagctca ccctcatccc tgtcggggta cttcctaaag aggaagtccg tggtaacaag
     6001 ttggagtacc gcatcaccca agtactctaa tctctcgttg tgagtgagac ccgaagagcg
     6061 gttctcattg atataagagc ggtgcgtaaa agcctgcctc aagagcgcct tgttgttgaa
     6121 gctaagtcca agcgtttcct cgaatttact gaaatccata tgttgagtta accagtttgt
     6181 gccaaaattt gcaaagcctt agtaactata ggcagtatat cgtcgtaaaa gttgagtcca
     6241 agtatgtcag ccagtctaaa ccacctgaca tcatcgagac cacccttctc ctccttcttc
     6301 tcaagtgtaa gctcttgata tggtgcctca gcgaggaagt atgtgacctg ctttttgatc
     6361 ttgcccttct ctgggtggaa ggtggtgtag gcgttctctc ccaatttctc cttcaccact
     6421 attggtacac caagctcttc ctctgcctcg cgtacgagtg cggcctcctc tgtctcctca
     6481 ccttcaagtt tgcccttagt aagagtccaa tgaccaaaga tgtcgtgtac aagtgctaag
     6541 taggtctctc cgttctcctt ggcataaacc actgctccag caagcttctc tagtgggagc
     6601 tcggagtctg ggacattacc atacttcttc ttaggctttg gttgatcgtc cttgcctggc
     6661 tcgcccatct ctctatatac tgcgccaaga accccgttaa caaacctacc actagtctct
     6721 ccaccataag atttagcgag ttcaattgct tcgttgatag ccaccttagg aggcacctca
     6781 ccccggtctg caaaaaggag ttcagcaaga cccattctta gaatgtttct gtctactatg
     6841 gaaatttttt ctattggcca gtcaggagcg gccttcacaa taatgttatc taaatcgttc
     6901 ttcttatcga ctacattttt caaaagattg ttcatgaacg gacggtcctt cgagtctgga
     6961 gcaaattcct ggacctgtct gccaaatact tcatatgcgt ctaccgactt accattcgag
     7021 atatcaaaat cccattcaaa aagcgtctgt aatacgacgg agcgcgaaag atgtcggtgt
     7081 gacataaatt aaagaactgt attgtctaga ccttacggcc tcgatattca cccgtcttag
     7141 ggtgagcacg gtgtcgcaaa tgaacagcac cactcttcgc cacggtcaaa gccgggttct
     7201 caagtgcatg atgtgagcgc ctgtttttag tatgcgcccg tgtgtgtctc attcgaacaa
     7261 ccatagggcc atattaccag ctttttagaa aaagaaaagc cccgcaccag aagtggagcg
     7321 aagctctctt ctcgtgcggg gcgctagaac tgatgcatct tggcggcgtg gagtatcggt
     7381 cgatgactcc agaagagccc cccatcctcg acctgaacca ggagctctga ctcagtccgc
     7441 cagaacggcg gagagtgctt gtccccatcg tcatctttct tctcttcggt gcggagctgt
     7501 cccccacagt cttcgaagcg agagatgaac gcgaccttct gatgaaagcc gggcttgtcc
     7561 cgattcttct gacgtgggcc tacccagaca cactctgagg ggttgggtag gatgagatca
     7621 gtttcttctt cggtctcccg gaggcaggtg tcgaagatca attcgcccgg tcggtcgttg
     7681 ttcgtgccgg cagggaactt gatctgcacc ccgtcaccag agtcgtactc catgacgagg
     7741 tactctctct cctcatcacc atctctacaa atgacggcga caccgagatg cgaatcgtcc
     7801 tctgaggggt tgatataacc caggtcttga ggagtcttca tctgacgcat cgttgctgtc
     7861 atacaagctc tcctatcaaa aggttctaga agtgggtata tcccaccttt gttcttatta
     7921 tactacataa tttgcctatt tgtcaagcct taagaatgtt ctttagaaaa ctcattctgt
     7981 ggaccgcaga tgaaccgtgc ttcttgatag cctctatgtg cgctttggtg ccgtagccct
     8041 tgtggatatc gaacttatac tctggatact ctgtagccaa cttcgtcata tattgatctc
     8101 ttgcaacttt ggcacatata gaggcgagcg agattaacgc gatcttctcg tcccctttaa
     8161 taattgtttc ttgattctta aattcttttg gagcatgtag cgagccgtct aaaaaaattt
     8221 ttgcatcctc atccacaccg agcctcttca tacacttact cataccgagc ttaatggcgt
     8281 agactattcc cctcttgtct attacatctg ctccgactag ttcgacagta aagttgagtt
     8341 tgccctccct cttcatctcc tcggccttag caaaccactc tttcctccct ttctctgtga
     8401 gcttcttaga atccttgata ccttcaaaaa aactcatatc gaaatcatga caaatctttg
     8461 ctactcctac cgccacagga ccagctagcg ggcctcgccc agcctcatct atcccaatta
     8521 tgtctgtcat cttctctata ttatactgtg tgcatgtcga agttcgtcca cctccacacc
     8581 cactctcact actctctcct tgaggcctta cccaagatct ctgaccttgt agctcatgcc
     8641 aagaagcatg agatgaaggc cttggcctta actgatgcag gcaacttata tggtgctatt
     8701 gagttctata aggaatgtaa ggatgagggg ctaaagccga tcatcggagt cgacgcctac
     8761 atcgcaccga ggaccaggct cgacaaagag gcccgtattg acgcatcacg ctctcgcttg
     8821 gtgctacttg cagagaatat agttgggtac aaaaatcttt tgaagttagt gtcactctcc
     8881 tatctcgagg gtttctatta caaaccaaga atggacaagg agatcctgga gaagtacaag
     8941 gacggactta ttgctatcct cccctcattc aacaatacag agcttgagga gcagataacc
     9001 tggtacaaaa aaacttttgg agacaagttc ttcgtcgaga tcactcatca ccccgagatc
     9061 gcgaatcacg aggataagca gagtgaaaga attgctctct caaaaaaact tggagtagag
     9121 atagttgcag cgcatgatgt ctattaccta gagaaagcag acagaccagc gcgtgagacc
     9181 ttgatgtcta tccagtcgca tatcggtgtt ggtcgtggca ggagttttga ggatgaggag
     9241 gacgacttct ccttcatctc gcccaaggag gcagagaaga gattcaaaag cactcccgaa
     9301 gcaatagaca acatagagaa gatagttgat atgtgtaatg tggaattgaa gctaggtgac
     9361 tgggtcttcc ccaaccttga aattcctgat ggaaagaccc ctgacgaagc tctgcgcgaa
     9421 attgtcgaag caggactcat aagaagaaag ctcgagaaaa caaaggaggt tgaagaacga
     9481 attgagtatg agctcaaagt cattagtgac aagggttacc ccaagtactt cctcgtcgtg
     9541 tctgacctac tccacttcgc ccatgagaac ggcatcctta caaacattcg aggatctgtg
     9601 gcgggctccc tcgttactta tcttgctggt attacaaatg tgaacccgct tgagtacaag
     9661 ctccccttcg aacgattcct caaccctgac cgtccttctg caccagatat cgacatggac
     9721 ttcgccgacg acaggcgcga cgaggttatt gattatgcga ggaaaaagta tggtgaggat
     9781 cgagtggccc aaattggtac cttcggcacc atgatggcga gaggtgcagt gcgcgatgtc
     9841 actcgtgcca tgggattcgc agtagccata ggagacaaga tcgcaaagtt gatacccatg
     9901 ggcgcacaag gcttccccat gactatcgat agagcgaaga aagagagtcc tgacttcaaa
     9961 aagctctaca cctctgaccc ggatgtgaag cgtattgtgg acatggccga gaagatcgag
    10021 ggcaacgcac gccacatctc ggtacacgca gcgggagtgg ttatatcccc ctctcctctt
    10081 accgactaca ccgcactcca atacgatacc aagggtgaga acaaggtgat cactcagtac
    10141 gacatgtact cgatagaaga agccgggcta ctcaagttcg acttccttgg tattaggaat
    10201 ctctcgatca ttgctgatgc tatcaatcgt ataaaaaaga ttgaaggtgt tgagatagat
    10261 atggatgaga tcccagtgga tgacaagaaa acttatgaaa tgcttgctcg tggcgagacc
    10321 gagggcacct tccaacttaa cggcgatggc atgacgaagg ccttgatgga acttaagccc
    10381 acagtgatcc acgacatcaa tgtcatggtg gcactatatc gtcctggccc gatggacaac
    10441 atccaagagt acatagcgag gaagcacaag aagaagcctg taacattcct ccatccaaag
    10501 atgaaggact tcctcgatac cacttatgga gtactcgtgt atcaagatga cctgttgatg
    10561 actgctatcg aagtggctgg ctactcgtgg ggcgaggtcg acaagtttag aaaggcggtg
    10621 ggcaaaaaga ttcctgaaga gatgaagaag caacatgtca tgtttgttga tggatgccaa
    10681 aagcatggag gcatgagtaa ggacaaagct gaggcacttt gggagctctt cgttcccttc
    10741 caaggatacg gattcaacaa ggcacatgca gcgagttacg gacacatcgc ttatatcact
    10801 gcgtacctca aagctaacta ccctgctatc tacatgtcag cagtactgac tgcagagtct
    10861 ggagatgtag agactatcgg cgtcatggtc aaagagtgca aacgtatggg tatagaagta
    10921 ctacccccat cggtaaacga aagttattcg caatttaccg taccacctcg cgagagtaag
    10981 gatgataaat acaaaataag attcggactt acaactatca agaacttcgg acaaggtgta
    11041 gcaacctcga tcatcgagga gaggaagaag ggtggtaagt tcaaatcatt aatagacttc
    11101 ctcgacagag ttaaagacaa gaatttaaat aaaaaatctc tagagagcct catcaaggct
    11161 ggtgcactcg acgactttgc cgagagagga gagatgctac aaaacctaga aactctactc
    11221 gcctaccaca aggagtcgag cgccaaggcg acgaaccagg actcactctt tagcggctta
    11281 accgggtcga gcgcactacc cccactacgt atgacaaagg ccgagcccat aaataagaaa
    11341 gaaagactca tgtgggagaa ggagctcctc ggtctctaca tcactgggca tccattggat
    11401 gagtttagcg aaaagttaga taaggcgaaa aataattcct taaacctcaa ggatgcccca
    11461 gagaatgccg agtgtgtcat aggcggtgtc attaccgatc ttcgtacgac acttaccaaa
    11521 gctggcgaga tgatggcctt tgctagacta gaggatctta agggcagtgt agatatggtc
    11581 gtcttcccaa aagtttatga ggagtacaaa gagttcctcc aaggcgacca gtgcattgcg
    11641 gtgaagggtc gcataggcaa gaagaacgac gaactcagct ttatcgtaga aaaagtaaaa
    11701 agtttataaa aaaaagcgag ttacctcgct ttaaaattaa ccctcttggc atgtgggtcg
    11761 acactgatca aacggttcga tgaatttctg aaactcggga tgcactacag caaagtgatc
    11821 gagaaactcg tcgaagctaa gacgaatggt ctcgatgtga acgagagcgg agtgccgaac
    11881 ggccatctgc gtcgaagcac ccattattgg gtagtcgaac cgactctccc tttcctctaa
    11941 tgactcggaa tcattggtgg aagggacatg cgcaatgagc acgacctcag tccttcctcc
    12001 aaagtgagct agttgccact cacacttacc atcgaagaaa gtgaatcgtg agaccactcc
    12061 caccctgcta ttccacttga gtctggataa gttctttttt gcgaactcaa cgacaagtcc
    12121 tgctcctagc tccatctcct tcttcattct gtcaaacatc tccttacgag ccatgaatga
    12181 cgaaagtttc gacacgcctc ctcctaccct cacgggtttt gtatatgagc atcactgccc
    12241 ggggaactgc ctgaacacat aaaaccattt atttgcttat tagtcaaaca aagaaaaacg
    12301 acaccttatc ggtgtcgtag aacttgtatc agcagcttcc gccgcaacaa caagtgatcg
    12361 aatcatggag agagagcagg gccggattca cgctatcccc gtaggtacga cggagaagtg
    12421 caagggtgtg gtcagcatat gctcgcttga ccgccctcgt gttctgcgtc acatccatgg
    12481 ggttgtgatc cacgaactcg ccgatgaact cctcacagaa ccttcgatag aaggctgtgt
    12541 tgaggatcat ggcatgccag atcgggtcga ctgactcgag catctggaga ggcttgccgt
    12601 cgagagcttc agggaccagc gagaaccact ggaggaaagc gtcaagcaac ggctcgatct
    12661 cttcgaccgt cttgaggttc gactcgcgca gcaccgtcgt gatgactgca gagaagtcga
    12721 ctgctttcat ccgacgatac aactccctcg cgtaaactgt acgctgatca tcggaaggct
    12781 gaccgatcgg cttgaaactc aaggagctgc gcattgaaac ctcataagtg aagggactga
    12841 agatcgcctg tttggcgagt tgttctacaa ttcatagtac atactaaata tcctgtagtc
    12901 aatacaaata aacaatcatc aacttatatg ttaaaatcac tcgtatgatg aatgttgaag
    12961 ataatgacca tagaaagatt ggtaaggagc tagatctctt caccttttct gatttagtag
    13021 gatcaggtct ccctctttgg accccaaaag gaacagtagt tcgaggtgaa ttggataagt
    13081 tcgtatggga attaagatca aaacacgact acagcaaggt cactatcccc cacataacta
    13141 agcgtgacct gtacgacaca tcgggccatt gggaaaaatt ctcagatgac ttattcaaaa
    13201 taaattcccg tgaaggtaag gagtacgcct tgaagccaat gaattgtcct catcacactc
    13261 aaatctttgc cagaaagcca cattcatatc gagagatgcc acaacgatat gctgagacaa
    13321 caatggttta ccgagatgag cagtcgggtg aattgggcgg tttaactcgc gttctttcta
    13381 tcacccaaga tgattcccat gtattttgta gaactacaca aatcaaagag gagttcaata
    13441 agatatggga tattgttgat aacttttata aaacatttgg tttcagtata cgagtacgtc
    13501 tatccttccg tgacccaagc gctcctgaaa agtacctagg tacaaaagaa atatgggaca
    13561 aagctgagag tgaactcaga gagattgcga cagaacgtgg cgctgattat ttcgaaggcc
    13621 ctggagaagc tgctctgtat ggtccaaagc ttgatttcgt agcgaagaat gctgctggac
    13681 gcgaatggca gattgccaca attcaactcg acatgaatct acccgaacga tttgatctat
    13741 cgtgtgttaa tgaaaaaggt gagaaggaac gcatagtgat gatccactct gctatcactg
    13801 gttctattga gcgttgtgtc gcaattatcc tagaacatct tgagggagtg ctccccctct
    13861 ggctctcgcc tgtacaagtt aaggtgctac ctattggaga aagtcatttt gattatgcta
    13921 agagtgtatt cgagtcgctt aagtccgaga gtatccgagc agagctagat gagtcgaacg
    13981 agtctctggg taagaagatc aggaatgcga agaccgacaa ggtcttctac accctagtta
    14041 ttggagacaa agaagttgag gataagacag tcacagtcga aggccgtgag gaaaaacttg
    14101 gtgcaatgtc tgtcgaagca ctaatctcta aaatcaaaga cgaaattaaa aatagaaaat
    14161 aatgcaaaac ggactttggt atttagttat tggttggata gtagggttca tatgctttac
    14221 aggtatttgg atatatgcat tcaatacttg ggggtttctt ataggattgg caatcggttg
    14281 gttacccgcc ttgataggcg gcttgatcgc cggattcctc tggccactaa ttttggttgc
    14341 catagttgtt atagcttttt tactttacta tcagttttaa acatattgac aagttccaat
    14401 aaaagatgac tacacagcgc tctatatatg gatattcaaa tttttgtgga taaagttata
    14461 gatatccaat accacaaaga gtaatatcta atggtaccta aatagcaaaa acaccctggg
    14521 aatcattttc tggagtaaac ataccggcgc cggcttaagg agtaattccc agccatccag
    14581 ttaaatgatt agtgggtgtt tttgtttggt gtattgaaca ccatccacat tctacttaag
    14641 caaaaatgat aaatcctgtg gataaacatt tcttttctca ttacaaatat tttgcgcaac
    14701 ctgtaaagat ccctctcgaa ttaaccctat aaacaaagct ataaactact tgtgctttat
    14761 aaaatttatt gtccgataca agaaatttga tccacttcaa ctaattagaa gtcggaaaac
    14821 cagccttaaa catccaaagt agctttcttt gcgtttgatt ggatgaaaga cttcctagat
    14881 gggacatcag tacccatgag gatgtcgaag accttgtcgg cgtcgcgacc gtcttctact
    14941 gttacttgct tcaagattct taccttaggg tccatggtgg tttcccaaag ttcctcagca
    15001 ttcatctctc cgagaccctt gtagcgttgt attgatacct tattagactt aaccttcttg
    15061 gttgtctctt cctctgcctc ctcttcttcc ttgatctcgg cattctcctc tatgacatcc
    15121 tcaacattga tgtccttgcc gacaatcttg ttcttctcct cgtctgtata tgcataatgt
    15181 acttccttac ccttcttgat cttatatagt ggtggttgag caatgtagat aaatccccca
    15241 tcaatgactt gtctgaagtg tctgtagaag agtgtaagaa gaagagtacg aatgtgtgcg
    15301 ccatcgacgt cggcatctgt ggctatgatg accttgtggt atcggagctt ctctaggtcg
    15361 aaggtgtcac caatagcagt accaagggca acaactaggt tcttgatctg ctcacttcct
    15421 agcattctat ctagtctagc tctctctatg tttaggatct tgccccttag tggaagtatg
    15481 gcttgtgtac gacggtctcg acccatcttg gcagtaccac ccgccgagtc tccctctact
    15541 atgaagagct ctgactcctc tgctgactta ctctgacagt ctgcgagctt gcctgggaga
    15601 gtcatgcctt cgagtgcgcc cttccttagc accgagtctt tagctgcctt cgctgcttta
    15661 cgagccttaa gagcgagcac taccttgttg atcattgcgc gagcgtcatc aggattctcc
    15721 tcgaggaagg tgctgaatgc ttcaccaaag atggttgtca ccatcgcctg tgcctctaca
    15781 ctccctagct tacccttggt ctgtccctcg aactggatct cacggagctt aaccgagata
    15841 actacagtaa gaccctcaag tacatcctcg ccggtgaagt tgtcctcact ctcctttacc
    15901 aaattattct tcctcgcgta agtattcaaa gttcttgtga gagcggtctt gaacccagtg
    15961 acatgagtac ctccctctga gttataaatg ttgtttgcaa atgggagtat gcgagacgat
    16021 atgtcgtcta catactggag agccacctcg actgtctcgt agtcgttgcc attggccgcc
    16081 ttctccacgt agaaaatatt tttgtgtatc ggcttctgaa gttcattctc aaagcggaca
    16141 agtgacatga gtcccccctc gaagtagaag gacattgatg gcacattgag agcgaggtca
    16201 cgaatgtaaa aggcatcctc tgattcgacc attgcttctt ctcttgcatc gatcactgtg
    16261 atcctgagcc ccttcacaag atacgcttgc tggcgcaggt ggttcaccac tcttttgaaa
    16321 tcgaaattag tgtctgggaa aatagttgga tccgggcgaa aggtgatgac agtaccctga
    16381 ttcttggttc cgcccgtctt cttcaccgca gccttcctct taccagcatt gtactcttgg
    16441 agataaacac caccgtcctt atgtacctga gcaatagtgt gagtcgagag cgcgttcacc
    16501 accgaggcac ccacaccgtg gagacctcca gaaactttgt atccttcacc tccgaactta
    16561 cctccagcgt ggagtgtggt catgattgtc tcgagtgctg agaccttggt ctgcttgtgt
    16621 acatcgacag gtattcctcg accgttgtcc accactcgaa tgtagttacc aggaaggagc
    16681 gagacctcga tatcattggc gaagccaccc atcgcctcgt cacgagagtt gtcaaagatc
    16741 tcccaaatga ggtggtgcaa gccgtcagga ccggtggtcc cgatgtacat acctggacgc
    16801 ttgcggacag gttccaagcc ttcgaggaca gtgatggatt cagcgctata attcgaggtt
    16861 ttcttgggct ctttagccat atttgtgccc ataattatag caaatatgag gcggaaatga
    16921 tagggtgctt atagagtcaa atagcacctt tccgtgccta cggcaccagc tctcggctga
    16981 tccgagaaat gcattaagtg aggtgtgcgg tgactagaga atccgtgagc cgaactctca
    17041 gcgttcacac
//
LOCUS       DAORIT010000013        17022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_566_length_17022_cov_50.410621, whole genome shotgun sequence.
ACCESSION   DAORIT010000013 DAORIT010000000
VERSION     DAORIT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 17022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17022
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_566_length_17022_cov_50.410621"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLD99_01720"
     CDS             complement(<1..232)
                     /locus_tag="PLD99_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRH25656.1"
                     /translation="MKIWVKARTGAKVDEVVPPPARLIPVEEEWYIVKTKAEPVEGKA
                     NEAITILLAEHFGVARSQVRLIRGATSKQKVFE"
     gene            complement(257..343)
                     /locus_tag="PLD99_01725"
     tRNA            complement(257..343)
                     /locus_tag="PLD99_01725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Ser,seq:act)
     gene            422..510
                     /locus_tag="PLD99_01730"
     tRNA            422..510
                     /locus_tag="PLD99_01730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:456..458,aa:Ser,seq:gct)
     gene            632..1093
                     /locus_tag="PLD99_01735"
     CDS             632..1093
                     /locus_tag="PLD99_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25657.1"
                     /translation="MRAAMISYNTFVAGNENGWKNQGDNNLLLLQNADGNMWGTSQFG
                     KTAEQWNDETKSLIDPLWADLERELASLDVVVFYVGSYGAERIIELAAEHGLTPDRAV
                     FVFCDCNMEKKSEVLRARGFENSRVIDCECGGHTTMRRVYTEILRYGRVLD"
     gene            1167..1460
                     /locus_tag="PLD99_01740"
     CDS             1167..1460
                     /locus_tag="PLD99_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25658.1"
                     /translation="MKNPYFNALGASLYIGVVGTFMHLMEKGYANTPDKWFSGIAFIS
                     LLTLSVATMGYFFVFVPLQMYLDGQKQESVRFFLKTLATFALITLVIFFFLFR"
     gene            1511..1747
                     /locus_tag="PLD99_01745"
     CDS             1511..1747
                     /locus_tag="PLD99_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25659.1"
                     /translation="MEYIVPLVLIVLIMLALGKLRVFDPVAKPEEVNRIFNDLTYLAN
                     GNRKLVWNIIAGLRAEKKRTPTLGEVVDRLVHGI"
     gene            1862..2698
                     /locus_tag="PLD99_01750"
     CDS             1862..2698
                     /locus_tag="PLD99_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRH25660.1"
                     /translation="MIQFIRSHKKRIVLGILVIVFIVIYKPLLSPPKDADWQEHLKVS
                     STAEFNGDLVTVRNVRNFRYSPTEADIHSNYYDKTYDLREVEKVWFVAEPFNEATYAA
                     HTFVSFEFKNGDFLAITIEARKTKNQDYSMLKGLLKTYPLMYIAADERDVVLLRANVR
                     KDKVYVYPVKTSSPENARLLLVDMLERMNELVERPAWYNTLFASCTSSIAKHVNNITR
                     GRVSTLSWQLYLTASADELALKSSLLDTDLTIDEAREKYQINKKSEKAGDVANYSTLI
                     RQ"
     gene            2782..3234
                     /locus_tag="PLD99_01755"
     CDS             2782..3234
                     /locus_tag="PLD99_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25661.1"
                     /translation="MLGDIVDMIPLFIHLAGFVIGLGAVTVIDLHGFLGRKSSYWSEA
                     TTRTHKVTKPLIWLGMLLAIIGGALFYQEEGLTLIPLIESGIAIILVLNGLFLSFSVS
                     PYLLKREMEGKSKELLPKSWQTKIALSLIVSDIGWWSALLLLVYYLSK"
     gene            3234..3749
                     /locus_tag="PLD99_01760"
     CDS             3234..3749
                     /locus_tag="PLD99_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011428229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25662.1"
                     /translation="MKKMVKVDNKMQRGYEYELVEPIGKNFHKDFRPDLTPKEMLELG
                     VFGGRYMRDCKNEYPRSWFTKAKLHKEGVPGHDKNLNFFKVDASQPLEVWQKKGWIHK
                     DDPRGWFEWYARYYMGRRLPKEDEKQIKRWIAMRRHVAQVVNKCRAGDYSCNTKQRQA
                     LLHWAYDSRKM"
     gene            complement(3752..4162)
                     /locus_tag="PLD99_01765"
     CDS             complement(3752..4162)
                     /locus_tag="PLD99_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007696214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRH25663.1"
                     /translation="MNINREKFVHVSLVLLRIVAGLLFLQHGGQKLFGWFGSGMSMGE
                     LPTLILVAGLLEAVGGLLIVIGAFVRPVALVLSGEMAFAYFMAHFANGFWPLQNGGEA
                     ATLFAFIFLFFAAHGGGLWSVDAWLKSRNMGVQM"
     gene            4249..5178
                     /locus_tag="PLD99_01770"
     CDS             4249..5178
                     /locus_tag="PLD99_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25664.1"
                     /translation="MAESIFIFVGSLFLVIRGSTLATKHATRLAQSFHISRYVIGFIV
                     VALISILPETLIAINSALAGIPEFGLGTLFGSNVADLTLIFAILIFHIGRGVRVSTKV
                     LSTVHFYPLFILLPLIFGLNGYYSRLEGIFLIVAGAFFYYKIFKGGVSDSPTQHTLVS
                     AKRSLLLLIFSIGLLLVGSHFTVSSASSLALALGISPVLIGMLVVGLGTTMPELFFSL
                     KSVEREEDSLAVGDLLGTVLADATIVVGILAVISPFAFPTKIVYITGGFMFLGAMMLI
                     NFMHTNKKISVKEGLILTLLWLTYVIVEFTVSS"
     gene            complement(5175..5507)
                     /locus_tag="PLD99_01775"
     CDS             complement(5175..5507)
                     /locus_tag="PLD99_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRH25665.1"
                     /translation="MKILIHLLLSALAIFVTAYLLPGVDVDTWATYFILAVVLGAINI
                     FIKPILTLLTLPLSIITLGLFSIVLNALMVMLADKIVPGFSVDGFVSALLFGLVLGVV
                     SFVFKKFD"
     gene            complement(5512..5862)
                     /locus_tag="PLD99_01780"
     CDS             complement(5512..5862)
                     /locus_tag="PLD99_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRH25666.1"
                     /translation="MFISLLDYYDIGLLLLRLTVGVIFIVHAVPKFKDKEWIPLGVLE
                     VLGGLAVLLGIFTQLAALGLAIIMVGAIYMKTRKWGVPFTTQNSTGWEFDLLILVSNI
                     VIITTGGGTLVLLS"
     gene            5934..6323
                     /locus_tag="PLD99_01785"
     CDS             5934..6323
                     /locus_tag="PLD99_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25667.1"
                     /translation="MSNIEHYWKEGKHIDLWSVVHTLSGVALGSILFYFEMDITIALV
                     IALMLFVGWEAVEVALGIKEHATNMVMDVVFDFLGFFGVLYFYRGLDMSINISIIIYS
                     CVALLFFSTWGFLAHKRRTREGESHLQ"
     gene            6388..7017
                     /locus_tag="PLD99_01790"
     CDS             6388..7017
                     /locus_tag="PLD99_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRH25668.1"
                     /translation="MQKARPAKRGRNNPEFHDEDNRLVARYLSGEEKAFELLYKKYRT
                     KLLRFIKDSIGDWEKSEDIVQETFIRVWRHLHRYDASRKFSTWIYTIASNLCRNELRN
                     RSRNIVTIFRKITEDETEDRWIEFEDPRQDPEIAIREREAKIVLATCLKQIPERRRRI
                     FVLRRLEGHTIAETAKIMMCPRGTVKSGVSRTEDEIVELSRELIRREHT"
     gene            7046..7384
                     /locus_tag="PLD99_01795"
     CDS             7046..7384
                     /locus_tag="PLD99_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25669.1"
                     /translation="MTIAKKQVIIQGRQLNPGVALLDHSPAWHTLNFKEALVASVLFV
                     ELVASGGIEFIDLNLVERETRMDLKTFMQTIDSLLDKNLIRVNEEGNLGLEGDLLRLF
                     NEEVLDRRGQ"
     gene            complement(7397..8041)
                     /locus_tag="PLD99_01800"
     CDS             complement(7397..8041)
                     /locus_tag="PLD99_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRH25670.1"
                     /translation="MKDFFKTLPENIVENFKGLNLLWNALAIVVTFFIVTLGVDWYYF
                     LITRSDIVTLFSWPAAVLGGLLPILLPLTLLFYGKRRFRIDLVILAWALAQAALIGSI
                     VSSFYKSLTGRIQPDLANTIVDSSRQFNFGFLEHGVFWGWPSSHTTIAFAMTLTLITL
                     YPDKRKLKVFSIIYAIYIGLGVSTTIHWLSEFIAGAIIGSVIGMIVGRSFKKRL"
     gene            8084..10240
                     /gene="recG"
                     /locus_tag="PLD99_01805"
     CDS             8084..10240
                     /gene="recG"
                     /locus_tag="PLD99_01805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRH25671.1"
                     /translation="MNLNDSLKTHFRILPVQEKALKRLGLLTVEDLLYHFPTRYTDTA
                     EVSSIASLKKGEVATIFGKITGLETKKAWTKKIPMSEACVTDGSERIKIVWFHQAYLA
                     KMLHEGGLVRIEGKVSERNGELYFSNPKVEQVDKLPETIGISLFGEESDVHALYPVYR
                     ETKGLTSNWIVHKIQNIFLSGVLDKLDDPIPEPILKKYSLPSLRTALLYIHTPKKITD
                     AEVARKRFAFEEIFFIQLEKQRTKHLFQVQNAFSINKKMEEVEKFIARFPFEATKAQR
                     RAVDAALEDFRRGHPMSRLLEGDVGSGKTAVAATLAYAITTSKPKGQNFGTLQTAIMA
                     PTEILAKQHFDSFIKLFRHMPINIALITGSGCKKFPSKVNPDIATDISRPQLKKWVAN
                     GEIAIVIGTHALIQKSVSFKHLALAVIDEQHRFGTKQRQSLVNKDHIAPHLLSMTATP
                     IPRTLALTLYGDLDLTLLDEMPEGRKKIITEIVLPNKRKETYEKIREELVRGRQAYII
                     CPRIDEPDPDKEFAVQAKSVKEEAKRLKKSVFPEYEIGIMHSKMSTSEKEQVMNEFKE
                     GRIDILVSTSVVEVGVNVENASVIVIEGAERFGLSQLHQLRGRVLRGTHQPYAYVFTD
                     STSQTSIERLRALKTAKNGFELAEHDLKQRGAGELVGLKQWGISDIGMEAIKNLKMVE
                     AARTEAVKIIETDPELKRHPKLGQRLESSDQKIHFE"
     gene            10410..10874
                     /locus_tag="PLD99_01810"
     CDS             10410..10874
                     /locus_tag="PLD99_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25672.1"
                     /translation="MSASVRFVAPSTCARLDSPFGTVNMGTVEFENTSTTASIVPKQL
                     AAQLSTTSIASVKMFHFGPEWRNADNSITANIDAQTDPVASYIQFGALPSIPPGGKFQ
                     TVLKGNIPNATAGTYTFAMIPDSVKVKTVAGDNVVVTMSANSFTLTTKASCP"
     gene            10939..11373
                     /locus_tag="PLD99_01815"
     CDS             10939..11373
                     /locus_tag="PLD99_01815"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HRH25673.1"
                     /translation="MNYGLLANLTALSHIATIVAVIAGLLLSFRYKRFRPWEAGALIL
                     IVILWSYYGNCPLTIIEQHFRDLAGQTGVNLTSVGFLPYYMHKLFDVSLASKTVQHST
                     FFTGGILFTASIEWFTPFFHMEIFKLRKFLKKVFKKKRLARA"
     gene            11475..11897
                     /locus_tag="PLD99_01820"
     CDS             11475..11897
                     /locus_tag="PLD99_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25674.1"
                     /translation="MIFMSLPLTGCTKEILAPVVEEKIELPPFRYPVENIAVGYTYVQ
                     DVNFYAPIGVAPQIEHKVTEDPKNPANLELWPPRLELVSNQLCEPNCTRMAWRLLVLY
                     KATQAGEATVTIHEASNPREKISFMVIATVWDDGAGKG"
     gene            12051..12857
                     /locus_tag="PLD99_01825"
     CDS             12051..12857
                     /locus_tag="PLD99_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25675.1"
                     /translation="MRQTLRVILLLFVLSIVFGCTSKEKPTIIEPEQPKQMCVVNIIM
                     NDSNPPSWIEMSDIHVSPSPPDTGEYYLGFPWWLRRLPDKYRLTLMIEERKTFWLAAR
                     NYYGYNIYPYGSAGANLLQVSDWNVTSPSDTTSGLEVASKTRYYSGFWRVTEFDALGR
                     PLWKEYGNDHIWRIKDVKNGMANVNYFSRQKAVYFLESSYEVKSLKCIRTGVTNLTSS
                     MGVRFRRTDFEEPLGVLSNPVKVPNITIKTEMYGTVSEARTALLLLMRDK"
     gene            complement(13010..13083)
                     /locus_tag="PLD99_01830"
     tRNA            complement(13010..13083)
                     /locus_tag="PLD99_01830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13047..13049),aa:Ile,seq:tat)
     gene            13145..13402
                     /locus_tag="PLD99_01835"
     CDS             13145..13402
                     /locus_tag="PLD99_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002833626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HRH25676.1"
                     /translation="MFAIINLEVISKLINLMLHKIAFILLIVGGLNWLLTGLMSSWDL
                     ANFVGSTVAMVIYILVGISAIVEIFGHKKMCRNCNASGSTM"
     gene            complement(13467..15407)
                     /gene="ftsH"
                     /locus_tag="PLD99_01840"
     CDS             complement(13467..15407)
                     /gene="ftsH"
                     /locus_tag="PLD99_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRH25677.1"
                     /translation="MSENKNQQNKKKIIIKPSGKGGRKMPESFARQAVLTVFIFFVIT
                     SLYSYINGGGEKPVEIPLSVLAQDVVGEKVQKIEVKGDELIVTYKDESQKVSKKEEGA
                     PLTTTLANYGVEKDALSGVEVSVISDRGAGFWILSLTPIILPIIFVIFFVWFLSRQVK
                     GAGMQAFTFGQSKARMIHPDDKVSRITFKDVAGAKEAKEELSEIVDFLKNPKKFLDIG
                     ARIPKGILLMGAPGTGKTLLARAVAGEAGVSFFSISGSEFVEMFVGVGASRVRDLFDL
                     AKKSAPAIIFVDEIDAVGRVRGGGMGGGNDEREQTLNQILVEMDGFEPNEKVIVMAAT
                     NRPDVLDPALLRPGRFDRRVTLDLPDRSDREEILKIHARKKPLAEDVNLVLIAERTPG
                     FSGADLYSLMNEGAILAARESRTKVSQFDLIRSIEKVMLGPERKSHILSKKEKELTAY
                     HEAGHAIVASVLPYADPVHKISIISRGRAAGYVLKLPIEDRKLQSKKEFLDDIAVALG
                     GYVVEKTIFGDLTTGPSNDLQVASALARDMVAKYGMSEKLGAVAFESQPGRPMFGQRL
                     EDKGYSEEVAAKIDVEVSKIIDDSMKKALEIITTHREALNDIAKTLIEVETIEQKQFE
                     EILMRNKIKPKKKLDIEHQPLV"
     gene            15453..15890
                     /gene="smpB"
                     /locus_tag="PLD99_01845"
     CDS             15453..15890
                     /gene="smpB"
                     /locus_tag="PLD99_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRH25678.1"
                     /translation="MSNYAENRKARFDYEFVEEFEAGLELTGAEVKSVRAGKISLVGS
                     FVSVRGGEAFLLNAEVQPYQPKNLKADYDPRRAKKLLMTKDELEKLTTAEATKGLTIV
                     PLTVYNKGRFIKCRVAIARGKKEFDKRESIKKRDTERDLKRSL"
     gene            15906..16300
                     /gene="ssrA"
                     /locus_tag="PLD99_01850"
     tmRNA           15906..16300
                     /gene="ssrA"
                     /locus_tag="PLD99_01850"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            16453..16956
                     /locus_tag="PLD99_01855"
     CDS             16453..16956
                     /locus_tag="PLD99_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25679.1"
                     /translation="MALCHDEREHAVGWSRWNPHASKASANTRKKKVKKRVNYLKSLT
                     VHQRLAYERVHLCLMAIGQYHEVLGVLIPISPDELHEHFPAFAKTLGFKGRFYRKGKD
                     GKCPAILIYLPEEKDAVYFITPNFSYSFEDDSEKKRVHSKTIRKAALKFCKAWLPKWP
                     WDEAKLS"
BASE COUNT         4787 a         4217 c         3814 g         4204 t
ORIGIN      
        1 tttcaaaaac cttttgcttc gaggtcgctc ctcttatgag tctcacctgc gaccttgcta
       61 cgccgaagtg ctctgcaaga agtatcgtga tagcctcatt cgccttccct tccacgggct
      121 cagccttcgt cttcactatg taccactcct cttctacagg tatgagtctt gcgggtggag
      181 gcaccacctc atccaccttc gcccccgtcc tcgccttaac ccatattttc atctctctat
      241 tatagaggca caaatgcgga gagagcgaga ttcgaactcg cggttccaat taaggaacga
      301 cagtttagta aactgttgat ttaaaccact catccatctc tccttgggcc acttaagagc
      361 cgattatagc agaaagctct ttcgctaaaa acggtctatt atgctaaatt gaccacattc
      421 tggagacgtg tcagagtggt ctaacgtacc tctttgctaa agaggcaggc cccttaaaag
      481 gcctcgaggg ttcgaatccc tccgtctccg caaaaattgg atgttggaat tatattaatt
      541 gacaaaggtg tattaatgtg ctaatatcta atgtagattc atcgggcaca agccccaaac
      601 gcacattcta aacaaacagg agtgccgaga gatgcgcgca gcaatgatct cgtacaacac
      661 gttcgttgcc ggaaatgaga acggctggaa gaatcaaggt gacaacaacc tccttctcct
      721 ccagaatgcc gatggcaaca tgtgggggac aagccagttc ggcaagaccg ccgaacagtg
      781 gaatgatgag accaagtccc tcatcgaccc gctctgggcc gatctcgaaa gagagcttgc
      841 atctctcgat gtggtcgtgt tctatgtggg gagctatggc gccgagcgca tcatcgagct
      901 cgcagccgaa catggcctca cccccgatcg tgcagtattc gtcttctgcg actgcaacat
      961 ggagaagaag tccgaggttc ttcgcgctcg tggtttcgaa aattcgcgag taatcgactg
     1021 tgaatgcggc ggacacacca ccatgcgtcg ggtgtacacc gaaatcctgc gttacgggcg
     1081 agtactagat taatctacga ctcactcccg ccctctttga atctttgatt cagagagggt
     1141 ttttctttgt tgttataata gagattatga aaaatccata ttttaatgcc ttaggtgcga
     1201 gtctatatat aggagtagtg gggaccttca tgcatcttat ggagaagggt tacgccaata
     1261 ctccagacaa gtggtttagt ggcatcgcct tcatctcgct cctcactctc tctgtcgcta
     1321 ctatggggta cttcttcgtc ttcgtccccc tacaaatgta tctagacggg cagaagcaag
     1381 agtcagtaag attcttcctc aaaactctag caacctttgc tctcatcacc ctcgtcatct
     1441 tcttcttcct ctttagataa gagagctttg tgtgatatct tggtattaga atctcccacc
     1501 aggagtggtt atggaataca tcgttccctt agtcctgatc gtgcttatca tgctagccct
     1561 agggaaactc agggtcttcg atccagtggc caagcccgag gaggtgaata ggatcttcaa
     1621 cgacctcact tacctcgcga atggcaatcg caagcttgtc tggaacatca ttgcggggct
     1681 ccgagcggag aaaaagcgga caccgacact tggcgaagtt gttgaccgcc tcgtccacgg
     1741 tatctagaaa cacccacaac tctttgggtg ttttttctta aaaaattgat actccattca
     1801 ctacaacacc tcaccttgtc atacaaatat cttctcttta tatgagtgtt aagattgata
     1861 aatgattcaa ttcataagat ctcacaagaa acgaattgtg ttaggtattt tggtaatcgt
     1921 attcatcgtt atatacaaac cccttctctc ccctcctaaa gatgcggact ggcaagagca
     1981 tttaaaagta tcctcaacag cggagtttaa cggggacttg gtcacagtta ggaatgttag
     2041 gaacttccgc tactcaccta ctgaggctga catacactcc aactactacg acaagactta
     2101 tgacctgcgt gaggtggaga aggtgtggtt cgtagcagag cccttcaacg aagcgacata
     2161 cgcggcgcac accttcgtat ccttcgagtt taagaacggc gacttcctcg ctattactat
     2221 tgaagcgagg aagaccaaga atcaggacta cagtatgtta aagggtctcc ttaagacata
     2281 tccccttatg tacatcgcag cagatgagag agatgtggtc ctgctccgag ccaatgtacg
     2341 caaagacaag gtctatgtct acccagtaaa gaccagtagc ccggagaatg cgcgcctact
     2401 cttggtagat atgcttgaga gaatgaacga gttggtagag agaccagctt ggtacaacac
     2461 actctttgcc agctgtacct cgagtatcgc caaacatgtg aacaacataa ctcgtggtcg
     2521 agtctctacc ctctcgtggc aactctatct caccgcatca gctgatgaac tcgctctcaa
     2581 gagtagtctc ttagatacag acctgacaat cgatgaagca agagaaaaat accaaataaa
     2641 caaaaagtca gagaaggctg gtgatgtagc aaactattct acacttatcc gccaataatc
     2701 cccggtggcc tcccctcagt atcggatgac aagccctccc cttgtcgtgt aactggggcg
     2761 agagtatgaa ataatctaga gatgctagga gacatagtcg acatgatccc cctctttatc
     2821 caccttgctg gatttgtcat aggactcggg gctgtgacag taatagacct gcacggcttc
     2881 cttggtagga agtcctcgta ctggagcgaa gcgactacaa ggacacacaa agtgactaag
     2941 ccccttatct ggctcggtat gttactagcc attatcggcg gagcactctt ctaccaagag
     3001 gagggtctca ctctcatacc acttatagaa agtggcattg ctatcatctt agtacttaac
     3061 ggattgttcc tctcttttag tgtaagtcca tatcttttga aacgagagat ggaaggaaag
     3121 tccaaagagc tcttacccaa aagttggcag acgaagatag cgctatccct catcgtctct
     3181 gatattggtt ggtggtccgc actactccta ctcgtatact acttgagcaa ataatgaaaa
     3241 agatggttaa ggtagataac aaaatgcaaa gaggttacga gtacgaactc gtagaaccaa
     3301 tcggcaaaaa cttccataaa gactttaggc ccgacctcac acccaaagaa atgctagaac
     3361 ttggagtctt cggcgggagg tatatgcgag actgcaaaaa tgagtaccct agaagctggt
     3421 tcaccaaggc caaactacac aaagaaggcg tgcccgggca cgataagaat cttaacttct
     3481 tcaaagtaga tgcatctcaa ccacttgaag tgtggcaaaa gaaaggctgg atacacaagg
     3541 acgacccgag gggttggttc gagtggtacg ctcgatacta catgggtagg aggttaccca
     3601 aagaagatga gaagcaaatc aagagatgga tcgcaatgcg taggcatgtc gcacaggtgg
     3661 tgaacaagtg tcgagcgggc gactactcat gtaacacaaa acagcgccaa gcgctgcttc
     3721 attgggctta cgattcaaga aagatgtagc cctacatctg cacacccata tttcgactct
     3781 tcaaccaagc atctacactc cacaagcctc caccatgagc agcgaagaag aggaagataa
     3841 aggcaaagag agttgctgcc tctcccccgt tttgcaatgg ccaaaaacca ttcgcaaagt
     3901 gcgccatgaa gtatgcgaac gccatctcac ccgagagcac aagtgcaaca ggtcttacga
     3961 aggcaccaat gacaatgagt aatcctccaa ccgcctcaag tagtcctgct actaggataa
     4021 gagttgggag ctcgcccata ctcatacctg agccaaacca accaaagagc ttctgacctc
     4081 cgtgctggag gaagagtagt cctgccacga ttcttaataa taccagcgat acatgcacaa
     4141 acttttctct attgatattc attttgttat taatcaatta atttagttac agctactact
     4201 ataccaaaat aagtgctgtg taagccaaat acaatatata attgagtgat ggcagaaagc
     4261 atctttatct tcgttggctc gctcttcctc gttatacgtg gctccacact tgccaccaag
     4321 catgcaacta ggctcgcaca gagtttccac atctcacgct atgtcatcgg atttatcgtc
     4381 gttgctctca tctccatact cccagagacc ttgatagcca ttaactcggc cttggctggt
     4441 ataccagagt ttggtcttgg aacactcttt ggctcgaatg tcgcggacct cacactcatc
     4501 tttgccatac ttatcttcca catcggacga ggagtacgcg taagtacaaa agtgcttagc
     4561 acagtgcact tctacccgct ctttatcctc ctcccgctta tctttgggct caatggttat
     4621 tactctaggc tcgagggtat cttcctcatc gtcgctggag ccttcttcta ttacaaaata
     4681 tttaaaggtg gagtaagcga ctcccccact cagcacactc tggtcagcgc caagagaagt
     4741 cttctactac tcatcttctc tatagggctc ttgcttgttg gatcgcactt taccgtctcg
     4801 tctgcaagct cgcttgcgct cgccctaggc ataagcccag tcctcatagg catgcttgtt
     4861 gtcggtctcg gcaccaccat gcctgaactt ttcttctctc tcaaatctgt agagcgtgag
     4921 gaagacagct tggcagtagg tgaccttttg ggcacggtac tagctgatgc aacgatagtt
     4981 gtcggtatac tagcggttat ctcgcccttc gccttcccca caaagattgt ttacatcacg
     5041 ggtgggttca tgttccttgg cgcaatgatg ctcatcaact ttatgcacac gaacaaaaag
     5101 atcagtgtaa aggaaggtct catcctcaca ctgctgtggc tgacttatgt gattgtagag
     5161 tttactgtaa gtagttaatc aaacttcttg aatacaaagg agacaactcc gagcaccagt
     5221 ccaaagagca aagcagatac aaagccatct accgagaaac ctgggacaat cttgtcagca
     5281 agcatcacca tgagagcatt taacactatg gagaataggc cgagcgtaat gatcgaaaga
     5341 ggaagagtga gtaaggtaag aattggtttg ataaagatgt tgatcgcacc caaaacgaca
     5401 gcaaggataa agtaagtagc ccaagtatct acatcaactc ccgggagtaa ataggcggta
     5461 acaaagatgg caagagccga gagtaagaga tgtattagaa ttttcatatt gttaggacaa
     5521 gagcactaga gtaccgccac cagtcgtgat gatgacgatg ttcgacacca agatgagtaa
     5581 atcgaactcc caaccagtcg agttctgtgt agtgaagggt actccccact tcctcgtctt
     5641 catatagatg gcaccaacca tgatgatagc gagaccaaga gctgcgagct gggtaaagat
     5701 gcccaaaaga accgcaagac cacctagcac ctctaggact ccaagaggta tccactcctt
     5761 atccttgaac ttaggtactg cgtggacaat aaatatcacc ccaaccgtca gtcttagaag
     5821 aagaagtcct atatcataat aatcaagtag agagatgaac attgggataa ttaagttatg
     5881 ttaataatga gattatacca aattgcttca tgcttgtgtg atatgcttcg aatatgagca
     5941 atattgaaca ctattggaaa gaaggtaaac acattgacct ttggagtgtg gtccacaccc
     6001 tgtcaggcgt cgcactcggg tctatcctct tctacttcga gatggatatt actatcgcct
     6061 tagtcatcgc cctaatgctc tttgtcggat gggaggctgt cgaagtagct ctcggtatca
     6121 aggaacatgc aaccaacatg gtgatggatg ttgtcttcga cttcttggga ttctttggcg
     6181 tcctatattt ctaccgcggg ctcgacatga gtattaacat ctcaataatc atatactcat
     6241 gtgttgccct cctcttcttc tccacctggg gattcctcgc tcataaaaga agaaccaggg
     6301 agggagaatc acatttgcaa taaggactga gtaggagcag aattctttat tggaactgca
     6361 cccaaccctt aagtaggaac tctgaccatg cagaaagcaa ggccggcaaa gcgagggaga
     6421 aacaaccccg agttccacga cgaggataac agactcgttg caaggtacct tagtggtgag
     6481 gagaaagcct tcgaacttct gtataaaaag tacagaacaa aactactccg cttcattaaa
     6541 gactccattg gagactggga gaagtccgag gacatagtcc aagagacttt tatcagagtt
     6601 tggcgacacc ttcatcgtta cgatgccagt agaaagttct ctacttggat ctacaccatt
     6661 gcatcaaacc tctgccgtaa cgaacttcga aaccgcagta gaaacatcgt caccatcttc
     6721 cgaaaaatca ccgaagatga gactgaggac agatggatcg aattcgagga cccgcgacag
     6781 gaccccgaga ttgctatccg agagcgcgag gcgaagattg tactcgccac atgcctcaaa
     6841 cagataccag agagaaggag aaggatcttc gtactccgga gactcgaagg tcacaccatt
     6901 gcagaaacag cgaagattat gatgtgcccc agaggcacag tgaaatcagg tgttagtcga
     6961 actgaagatg agatcgttga actttccagg gaactgatca ggcgcgagca tacctagtgg
     7021 tatgcttttt cttttataat tttgtttgac tatagctaaa aaacaggtta taattcaagg
     7081 tagacaactt aaccctggag tagctttgct cgatcattca ccggcatggc acaccttaaa
     7141 cttcaaggaa gcgctggttg cctcagtcct cttcgtcgag ctcgtagcct ctggaggaat
     7201 agaattcatc gatctgaacc tcgttgagag ggagactcgc atggacctaa aaaccttcat
     7261 gcagacgata gactctctac tcgacaaaaa cctgatcaga gtaaatgagg aaggcaatct
     7321 cggactcgaa ggagaccttc ttaggctctt caacgaagag gtgctcgacc gccgcggaca
     7381 gtagtccgcg gctttttcat aatctttttt tgaaacttct gccgacgatc ataccaataa
     7441 cagaaccaat aatagccccg gcaataaact ccgataacca atggatagta gtcgagactc
     7501 ccaaacctat gtaaatagcg tagataattg aaaaaacttt aagtttcctc ttatctggat
     7561 acaaggtaat gagggtgaga gtcatcgcaa aggcgatagt ggtgtgtgat gatggccaac
     7621 cccaaaatac tccatgttcc aaaaatccaa aattaaattg cctactagaa tcgacaatcg
     7681 tgtttgccaa atccggttga atacgcccag tcaatgactt atagaaagag gacacgatcg
     7741 agcctataag tgcagcctgt gcaagtgccc aggccaagat aaccaaatct atcctaaacc
     7801 tgcgcttacc ataaaaaagt aaagttagag ggagcaagat aggcaacaac ccaccaagca
     7861 cagctgccgg ccatgaaaac aaggtgacaa tgtcgctcct agttattagg aaataatacc
     7921 aatcaacacc caaagtaacg ataaaaaaag taacgactat agcaagggcg ttccaaagca
     7981 gattcagacc cttaaagttc tctactatat tctccggcaa tgtcttgaag aaatccttca
     8041 tataatagat cctattctat ccttttaaag gtagaatgga aaaatgaatt taaacgacag
     8101 tctcaaaaca cacttccgta ttctccctgt gcaggagaaa gcattaaaga gacttggtct
     8161 cctaactgtc gaggacctct tgtaccactt ccccactcgc tacaccgaca cagctgaagt
     8221 aagtagcatc gcctcgctta agaagggtga ggttgcaacg atctttggca agatcactgg
     8281 actcgagaca aaaaaggctt ggaccaaaaa aatacccatg tccgaggctt gtgtgacaga
     8341 cggatccgaa agaataaaaa tagtttggtt ccaccaagcg tatctcgcca agatgctcca
     8401 cgagggaggg ttagtacgca ttgagggtaa agtaagtgag agaaacggtg agttatattt
     8461 ttcgaaccct aaagtagagc aggtagacaa gctacccgag acaatcggga tctcgctctt
     8521 tggtgaggag agtgatgtac acgctcttta ccccgtatac cgggagacta agggtctcac
     8581 tagtaactgg atcgtgcaca agattcagaa tatcttcctc agcggtgttc tcgacaaact
     8641 agatgaccca ataccagaac caattttgaa aaagtattct ctcccctctc tccgcacggc
     8701 actgctctat atacatacac caaaaaaaat taccgatgca gaagtagcaa ggaaaagatt
     8761 tgcttttgaa gaaatattct tcatccaact tgagaagcaa agaacaaaac acttattcca
     8821 agtacaaaac gctttctcta tcaacaagaa aatggaagag gtggagaagt ttattgctcg
     8881 cttcccattc gaggcgacta aggctcaaag aagagctgtc gatgcggctc tagaggactt
     8941 ccgacgtggg cacccaatgt ctagactcct cgaaggagat gtaggtagtg gtaagactgc
     9001 tgtcgccgcg actctcgcgt atgcaataac cacttctaaa cctaagggac aaaactttgg
     9061 cacgctacaa actgcgatca tggcgccgac cgagatcctg gccaagcaac acttcgactc
     9121 ttttatcaaa ctctttagac acatgccaat aaatattgca ctcatcactg ggagtgggtg
     9181 caagaagttc ccaagtaaag taaacccaga catagcgacc gacatctctc gacctcagct
     9241 caagaagtgg gttgcgaatg gtgagattgc tattgtgatt ggtactcacg ctctaataca
     9301 gaagtcagtc tccttcaaac atcttgcgtt agcagtcata gacgaacaac accgcttcgg
     9361 taccaaacaa agacaaagtc tggtgaataa ggatcatata gctccccacc ttctctccat
     9421 gacagcaacc cctatacccc gaacccttgc tcttactctt tatggagacc tagacctcac
     9481 cctactagac gagatgccag aaggcaggaa gaagataatc accgaaatag tcctaccaaa
     9541 caagcgcaaa gaaacttatg agaagatacg cgaggagtta gtccgtggac gtcaggctta
     9601 catcatttgc ccgaggattg atgagccaga ccctgacaaa gagttcgctg tgcaggccaa
     9661 gtcggtcaaa gaggaagcta agaggttgaa gaagagtgtc ttccccgagt acgagatagg
     9721 tataatgcac tcaaagatga gtacgagtga gaaggagcaa gtgatgaatg aatttaaaga
     9781 aggaaggatc gacatcctcg tctcgacctc ggtagtggag gtcggagtga atgtggagaa
     9841 tgcgagcgta attgtaattg aaggtgcaga gcgcttcggc ctctcacaac tccatcaact
     9901 tcgagggcgt gtgctccgcg gcacgcatca accatatgct tacgtcttca ccgactcaac
     9961 atcacaaacc tctatagaaa gactacgcgc cttaaagact gcaaaaaatg ggttcgaact
    10021 agcagaacat gatcttaaac agcgtggtgc tggagaactc gtgggtctca agcaatgggg
    10081 aatatctgat atcggcatgg aagctatcaa gaacttaaaa atggtagagg ctgcacgtac
    10141 cgaggcggta aaaataattg aaaccgatcc agaactcaaa agacatccaa agttaggtca
    10201 aagacttgaa tcctctgacc aaaaaatcca ttttgaataa ttattgacaa aaacaataaa
    10261 taggagtcta atggcaacca gatacgcagt tctccaaacc aattgacccg gaggggccaa
    10321 tgatccgcta catccagtgc acgctcatcc tctgtctcac tgccatcctg gcagcctgcc
    10381 agagcacaac cgaaccgaag gaagacgtgg tgagcgcctc tgtgcgcttc gttgcgccga
    10441 gcacatgtgc tcgactcgac agcccctttg gtacagtgaa catgggcacc gtcgagttcg
    10501 agaatacctc gaccacggcg tctatcgtac cgaagcaact cgccgcacag ctctcgacca
    10561 catcgatcgc ttcggtgaag atgttccact tcggaccgga gtggcggaat gccgacaaca
    10621 gcatcaccgc taacatcgac gctcagacag accctgtggc gtcttacatc cagttcggag
    10681 cactgccttc gatcccgcct ggtggcaagt tccagaccgt gctcaaggga aatatcccga
    10741 acgccacggc cggcacctac accttcgcga tgatcccaga ctcggtgaag gtgaagactg
    10801 tggccggcga caatgtcgtg gtcaccatga gtgcgaacag tttcaccctc acgacaaagg
    10861 ctagctgtcc gtgatcgaca caccatttga attcgaaagg attcaaatgg tgtttttctt
    10921 tatgttagga ttattaaaat gaattatgga ttactcgcga atttaactgc tctctctcac
    10981 attgcaacta ttgttgcagt aatagctgga ctactactct cctttagata caaacgcttc
    11041 cgaccttggg aggctggagc tttgatactt attgtcatcc tctggtcata ttacggtaac
    11101 tgccctctca ctataataga acaacacttc cgcgatcttg cgggacaaac tggggtcaat
    11161 ctgacatcag tcggcttcct cccctattac atgcacaaac tctttgatgt atctctcgca
    11221 tcaaaaacag tacagcactc aaccttcttc actggaggta tactcttcac cgcctctatc
    11281 gagtggttta cacctttctt ccatatggag atatttaaac ttagaaaatt cttgaaaaaa
    11341 gtgttcaaaa agaagcgttt agccagggct tgacaaaatg ggtatttgtg taatataata
    11401 agtacagtag ttccccagtc acacacaccc tagttcggag gcaacacatg cttcgtcgct
    11461 tgctcttgct cgtcatgatc ttcatgtcgc tcccgctcac cggctgcacc aaggagatcc
    11521 tcgcgcccgt cgtcgaggag aagatcgagc tcccgccgtt ccgctacccg gtcgagaaca
    11581 tcgccgtcgg ctacacctac gtgcaggacg tgaacttcta cgccccgatc ggcgtggcac
    11641 cgcagatcga acacaaggtc accgaggacc ccaagaaccc ggcgaacctg gagctctggc
    11701 cgcctcggct cgaactggtc tcgaatcagc tctgtgagcc caactgcacg cggatggcat
    11761 ggcgactcct tgtcctttac aaggccaccc aagccggcga agcgaccgtg accatccacg
    11821 aagcctcgaa cccgcgggag aagatctcct tcatggtgat cgccacggtg tgggatgatg
    11881 gagccggcaa aggctgagtc ctgagctcgg ccaagatcaa aagccctcga cagtagaaat
    11941 gctgtcgagg gtgctttttt tataaaaatt ctgtttgaca tataaatcca taagtagcag
    12001 tattcgacta gaagttcttc ttatacactc ccctgacttg gagacatgac atgcgtcaga
    12061 ctctgagagt catccttcta ctcttcgtgc tcagcatcgt atttggctgt acttcgaaag
    12121 agaagccgac catcatcgag cccgagcagc cgaagcagat gtgtgtggtc aacatcatca
    12181 tgaacgactc gaatccgcca tcttggatcg agatgagtga catccatgtg agccctagtc
    12241 cgccggacac aggtgagtac tatctgggct ttccttggtg gttgcgtcga ctacctgaca
    12301 agtacagact gaccctcatg atcgaagagc gtaagacctt ctggctagct gcaaggaact
    12361 actatggtta caacatctac ccttatggca gtgctggggc aaatctccta caagtctctg
    12421 actggaatgt gacaagccca agtgacacga caagcggcct agaagtagcg agtaaaactc
    12481 gctactactc gggcttctgg agagtcactg agtttgatgc cttgggcaga cccctctgga
    12541 aagaatacgg caacgaccac atctggcgca ttaaagatgt gaagaatgga atggcgaatg
    12601 tgaattactt cagccggcag aaagcggtgt acttcctcga aagttcgtat gaagttaagt
    12661 cactcaaatg catccgtact ggtgtgacca atctcacatc aagcatggga gtcagattca
    12721 ggaggactga ctttgaggag cctctgggtg tactatcgaa ccctgtgaag gtgcccaaca
    12781 tcactattaa aactgagatg tacggcactg taagcgaggc aaggactgca ctccttctgc
    12841 ttatgcgaga caagtaggac cccctgcccc gaactattgt tcggggcttt ttcttgtccg
    12901 cccgcttgga tagaacctct gggaaaccca cggttttccc aaaccttcct ccacgggaga
    12961 ccaaaatgtt ctcccgatcc ctctctagtt cgaatccaag ctaaaagtgt ccgcccgctt
    13021 ggattcgaac caaggacccc agaggtataa gctctgtgct ctaaccaact gagctacggg
    13081 cggttgatta cacaagaata tctcaaaaga cctctgtgga caaacttcat ctataacaca
    13141 agccatgttt gctataatta accttgaggt tataagtaaa ttaattaatt taatgcttca
    13201 caaaatcgcg tttatcttgc tcatcgttgg aggtcttaac tggcttctaa ctggacttat
    13261 gtctagttgg gatctagcta acttcgttgg ttcaacagta gctatggtta tctacatcct
    13321 cgtaggtatc tcagctatcg ttgagatctt cggtcacaag aagatgtgcc gaaactgcaa
    13381 cgcttctggt tcaacaatgt agttctaggt ttattcttaa gaatcaaaaa accgccactc
    13441 tggcggtttt ttgattccac acttttctaa actagaggct gatgctctat gtcgagcttc
    13501 ttcttaggtt tgatcttgtt cctcatcaaa atctcctcaa actgcttctg ctcgattgtc
    13561 tctacctcga ttaaagtctt cgcaatatca ttcaatgcct cgcggtgagt ggtgatgatc
    13621 tctaacgctt tcttcattga gtcatctatt attttagaaa cctctacatc aatcttggct
    13681 gctacctcct cggagtaacc cttgtcctca aggcgttgac caaacattgg tcgacctggc
    13741 tgactctcaa aagcaactgc gcccagcttc tcactcatgc catacttagc caccatatcg
    13801 cgcgcaagtg ctgaggcgac ttgcaggtcg ttcgatggac cagtagtaag gtcgccgaag
    13861 atcgtcttct ccaccacata gcctccgagc gctactgcaa tgtcgtctag gaattccttt
    13921 ttactttgta gcttcctgtc ttctatgggg agcttgagta catagcctgc agcacgacca
    13981 cgactaatga tagaaatttt atgcacagga tctgcatacg gaaggacaga agcgacgatg
    14041 gcgtgacctg cttcgtggta ggcggttaat tctttttctt tcttgctcaa tatatgactc
    14101 ttcctctctg ggccgagcat caccttctct attgatctaa taagatcaaa ctgtgagacc
    14161 ttggtacgac tctcgcgtgc tgccagtatc gcaccctcgt tcatcaaaga gtaaaggtct
    14221 gcaccagaga agccgggagt acgctcggca ataagaacca agttcacatc ctcggctaga
    14281 ggcttcttcc ttgcatggat tttcaaaatt tcttctcgat cagagcggtc aggcagatca
    14341 agagtgacgc gacggtcgaa acgaccaggt cgaaggagtg ctgggtcgag gacgtcaggc
    14401 ctgtttgtcg cagccatcac gatcaccttc tcgttcggct cgaagccgtc catctcgacc
    14461 aagatttgat tcaaggtctg ctctctctcg tcattgcctc cacccatacc accaccacgc
    14521 accctaccca cggcatctat ctcatcaacg aagataatag ctggggccga cttcttcgcg
    14581 aggtcgaaga ggtctcgcac acgagatgca ccaacaccca caaacatctc tacgaattca
    14641 gatcccgaga ttgaaaagaa ggacacccca gcctcacccg ccaccgcacg tgcgagtagc
    14701 gtcttaccag taccaggtgc acccatgagt aaaataccct taggaattcg tgcacctata
    14761 tccaaaaact tttttggatt cttcaagaag tcgacaatct cgctcaactc ttccttagct
    14821 tcctttgctc cagccacatc cttgaatgta atgcgactca ccttgtcgtc tgggtggatc
    14881 atcctcgctt tagactgtcc gaaggtaaaa gcttgcatcc ccgcaccctt aacttgtctc
    14941 gaaaggaacc agacaaagaa gatcacgaaa atgattggga gaataatggg cgtaagcgag
    15001 aggatccaga aaccagcacc acggtccgag ataacactta cttctactcc cgagagagca
    15061 tccttctcta cgccatagtt agcgagagta gtggtgagag gcgcaccctc ctccttcttc
    15121 gaaacctttt gtgattcatc tttataagta acgataagtt cgtcaccctt aacctcgatc
    15181 ttttgtacct tctcccccac aacatcttga gcaaggaccg agagtggtat ctcgactggt
    15241 ttctctccac caccattgat ataagagtag agactggtaa tgacgaagaa aataaagacg
    15301 gtgaggacgg cctgtctagc aaagctctct ggcattttcc taccaccctt accacttggt
    15361 ttgatgatta tcttcttctt attctgctga tttttgttct ctgacattca ccgaattata
    15421 ccgaaaaatc taggttttgc tatgattgct tcatgagtaa ttacgcggag aacaggaagg
    15481 ctcgcttcga ctacgaattc gtcgaggagt ttgaggctgg gctcgagctc acaggagcag
    15541 aggtgaagag tgttcgagct gggaagatca gtctagtcgg ctcattcgtc tctgtaagag
    15601 gcggtgaggc cttcctcctc aatgcagaag tgcaacctta ccaaccaaaa aaccttaagg
    15661 cagactacga tccaaggcgt gcaaagaaac tgcttatgac caaagatgag ttggaaaagc
    15721 tcaccacagc cgaggccacc aagggcttga caattgtacc acttacagtg tataataaag
    15781 gtaggtttat caagtgtcgc gtggctattg ctcgaggcaa gaaagagttc gacaaacgcg
    15841 agagtatcaa aaagcgcgac accgagagag acctaaagag aagtctgtaa cttacatttc
    15901 aatacgggga tgatcggcct agacaaggac gaccatcaga gtgatgcgtg agagagccga
    15961 gaggactctt taatccctct caaaactcaa ctgcaaaaga ttcttttgct aggatttctc
    16021 catacttcgg tatgaaggac ttccagttcg catacgctag agcctaaccg ctcgcctgtg
    16081 cgcatggcct cagcctttgt ccaaggggtt gaaagctgtg gaatactttg gactcgaagt
    16141 agagcctcct cactctcctt ctaacactcg aggatagagc attaagagct ttcgtctgtt
    16201 ttgtatctta atgtttgaaa ccaaatgaag cagtctacct catgtagacc tcactctgat
    16261 tcatctttgc acggcggttc gattccgccc atctccacca atttacaaaa ccatcctaat
    16321 gggtggtttt gtattaaagg aacttgatga gtggaaggta tagaactccg acagtgtcgg
    16381 agtttttctt tttacatttt ttaattttta gagtattcta aaaatagaaa cgaaccaaca
    16441 cttggagaca aaatggcact gtgtcatgat gagcgcgaac atgcagtggg ctggagcaga
    16501 tggaaccccc atgctagtaa ggcgtccgcg aacacgcgaa aaaagaaagt gaagaagcgc
    16561 gtcaactacc tcaagtctct aacggttcac caaaggctgg cctacgaaag agttcatttg
    16621 tgtctcatgg ctattggtca ataccatgag gtattagggg tattgatccc aatttctccg
    16681 gatgaacttc atgaacactt ccccgccttt gctaagacac tcgggttcaa gggccgtttc
    16741 tatcgaaaag gtaaagacgg taagtgccca gcgatactga tctatctacc tgaagaaaaa
    16801 gacgctgtgt actttatcac tccgaatttt agctacagtt tcgaggacga ttcagaaaag
    16861 aagagggtcc actcaaaaac cattcgcaag gcggcactca agttctgcaa agcttggctc
    16921 cccaaatggc cctgggatga agcgaagctg agctagatac aagactccga cagtgtcgga
    16981 gtttttcttt tgcatttttt aattttgaga gtaggctggt tg
//
LOCUS       DAORIT010000014        16203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_605_length_16203_cov_50.043659, whole genome shotgun sequence.
ACCESSION   DAORIT010000014 DAORIT010000000
VERSION     DAORIT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_605_length_16203_cov_50.043659"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..648
                     /gene="nusG"
                     /locus_tag="PLD99_01860"
     CDS             103..648
                     /gene="nusG"
                     /locus_tag="PLD99_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRH25680.1"
                     /translation="MSKQKISEGRNWYVIHTYAGYENAVERNLKQRIESLGMEDKIFN
                     VVVPTEKKIKIKGGKRVEEEEKIYPGYILVDMIVTDDSWYVVRNTPRVTGFVGSGVYP
                     VPIDKSEVDSLFARMNTDKVMHNINLVPEDGVLIVDGPFKDLEGKVSEIDQERGKVKV
                     LVSMFGRETPVELDFLQVKKM"
     gene            672..1094
                     /gene="rplK"
                     /locus_tag="PLD99_01865"
     CDS             672..1094
                     /gene="rplK"
                     /locus_tag="PLD99_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRH25681.1"
                     /translation="MAKKVTKILKLQIPGGKAVPGQALGPVLGGAGINIGEFVKRFND
                     ETKDRVGDIVPTVVEVYDDRTYGMTYKTEPASNLILKVLGKEKGSGKPNTAKVGTITK
                     AQVKAIAEKKMADLNATDIDAAMKIIEGSARSMGVDVK"
     gene            1201..1899
                     /locus_tag="PLD99_01870"
     CDS             1201..1899
                     /locus_tag="PLD99_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HRH25682.1"
                     /translation="MRKNKLFAVIHVEDEKQAHRNAVVALNNGADGIFLINHRVSVLE
                     LIRSYRYVRERHKASWIGLNLLGFSLTQAVHNKRLGDSLPRDIQALWCDDVRYTEGLG
                     PERAVYFADRLHFELQVATNHALLFGGYAFKYQEPVSDLVLGARLLAPHVGVITTSGP
                     ETGTPPTTEKIELIRKGMGDGAKLAVASGMTPENVDPFLDLVDYFLVATGISESFTEL
                     DPVRVRLMADKMRR"
     gene            2032..2670
                     /locus_tag="PLD99_01875"
     CDS             2032..2670
                     /locus_tag="PLD99_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRH25683.1"
                     /translation="MTDNKELAARLGQIVIHARKETDIDTRSRFLQNDDEVRFLVDRA
                     RNLGLPVVLTQGTFDLVHIGHARYVREAKNRGHLLIVGIDDDEKARERKGENRPVVPF
                     IERCEMMCHLRYVDAVAMKKHDDPKWHMIKLVRPDVLVAVEGTYSEEEKMELEKICDQ
                     VVVLPRQAETSTSAKVRTLVLDGAENLTRILAQKLPDFIHRIYQDLKKNGGA"
     gene            2667..3515
                     /locus_tag="PLD99_01880"
     CDS             2667..3515
                     /locus_tag="PLD99_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HRH25684.1"
                     /translation="MRKALVAYVPVLHQGYLDFFKRHKDAEELFIFSQPIIDKFDWLK
                     RKDIRAVNPEEMLLALRALGIAKQVTLLDDEQIAKLRDSNISLVFADESESRRVVEEF
                     FGESQVSFDSVFLRWDKPRVLDEKNAQGFPVTSEEVHRKFMDKASLASERSSDWWIHV
                     GAVLVSGDRVVVGYNRHTPSEHTPYALGDPRSLFNKGIAVELTSALHAEGAIVGAALR
                     EGVQTKGASLYITHFPCPYCANILAETGIAEIYFRTGYSMLDGADLLRKAGIKLFQVV
                     DEKSPE"
     gene            complement(3528..3977)
                     /locus_tag="PLD99_01885"
     CDS             complement(3528..3977)
                     /locus_tag="PLD99_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRH25685.1"
                     /translation="MKRLMHITNARVPEQRAQMEDIRRRGICPFCRENFEADHKAPIL
                     FENHHWLLTKNDYPYEGTRLHLLLVHKRHIEDLSGMTHASLAALSPVLKWAKKRFRIP
                     GGTFLMRFGDMRYNGATIAHLHAHIVVGVREGKNTQDIKKKLGYARK"
     gene            complement(3974..4474)
                     /locus_tag="PLD99_01890"
     CDS             complement(3974..4474)
                     /locus_tag="PLD99_01890"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRH25686.1"
                     /translation="MLNIIVAISQNGVIGKNGGLPWHLPDDLRWFKGMTAGYPVVMGT
                     KTFDSIIKAIGKPLPGRESVVLTRRPRARRCDGVIFTDNFDMVVEMSKTRGVFVIGGA
                     DLFRLALPFASKIYLTQVHTEIEGDTVFPEWNKGEWILEYSDDHPVDEKHEFPFTWEI
                     YSRRAT"
     gene            complement(4483..5367)
                     /gene="thyA"
                     /locus_tag="PLD99_01895"
     CDS             complement(4483..5367)
                     /gene="thyA"
                     /locus_tag="PLD99_01895"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRH25687.1"
                     /translation="MLPFSDRTVDTQYRDRLRLILEQGELIGETQQGVGALTYMAPPP
                     MHFDLSNGVPLITERKIPFWKAAVGELLAFANGVRTQKGLEEFGCSWWDKWLTAEKCA
                     KRGLEAGDLGPGGYAAMHDFPTAEGNPFNQIKHLVEQISEKPQLRTHFVSPWVPQYVG
                     RGKGKKQRVVVAPCHGWMHVRIIPHKGLVLHMFQRSADFPVGVPANMIQYAAFTLALA
                     HVTGHKPYKFIHSFSDAHIYVDQVEQVKEMLSREPRRFPTMTLNTDKKDIFAIRSDDF
                     VISDYDPHPGIREIPVAV"
     gene            5461..7044
                     /gene="argS"
                     /locus_tag="PLD99_01900"
     CDS             5461..7044
                     /gene="argS"
                     /locus_tag="PLD99_01900"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRH25688.1"
                     /translation="MKETIQVLIRKALKELAIEDKGFIVEHPTDLRMGDYSTNVAIKN
                     GLKKQEIFEYIKSHKPEEIERVEMVGPGFINFYLSKKFFAESLKQIIKAGDKFGRTKK
                     FKGEKVMVEYTDPNPFKAFHIGHLMSNAIGEAVSRVIEWNGADVRRANYQGDTGMHIA
                     MAVWGYFHGEWSTAYAIGAKAYVDNLEARKEIEEINKKIFERSDKSITKVYDEGRKAS
                     LEYFESIYKKLGTKFDYYFFESEAGELGKKLVEKNVGKIFEESDGAIVFHGEKFDPSL
                     HTRVFVNRHKLPTYEAKDLGNAVNKYKKYKYTKSVVITGNEIKEYFKVMLTALSQMYP
                     DLSKSTKHLPHGMLRLPTGKMSSRTGDVITAEDLIKDVSAKVKDSEPLSIAAIKYMIL
                     RQSIGNDIVFDFDKSVSTEGDSGVYLEYAHARATSVLDKATTSWSIKGERGETAEIER
                     LLYRLPEIVERAGEDYSPQFVTTYLTELASSFNNFYAHEQVNSDSPETKYRLAIVEAF
                     KVVMKNGLTILGIPVLDRV"
     gene            7101..7733
                     /gene="gmk"
                     /locus_tag="PLD99_01905"
     CDS             7101..7733
                     /gene="gmk"
                     /locus_tag="PLD99_01905"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRH25689.1"
                     /translation="MPGDVVAFSGPTAVGKNRILDRFWATKDSAKRVAHTTRQPRVGE
                     VNGDNYHFVTREEFEVLVAQGEFLEHAEVYGNLYGTTRLGLSEVVASGKNVFFDLDIQ
                     GARQLKAKMPEVISIFVLPPSIAELERRLRDRKTEDEETILLRLKKVEGEIRQAPRFD
                     YFVVNDDLDKVVHEIQVLLGILCLGKRPPPERFRNTDHIDKLLLELVASQ"
     gene            7779..11153
                     /locus_tag="PLD99_01910"
     CDS             7779..11153
                     /locus_tag="PLD99_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRH25690.1"
                     /translation="MPEDKGMTRSEREEKVLQFWKENKIFEKSLEKPSPKGEFVFYEG
                     PPTANGKPGIHHLESRAFKDAVPRYKTMQGYKVPRKAGWDTHGLPVEIESEKKLGLKS
                     KREIEEFGVALFNAECRRSVLSYIDLWSKFTERIGYWVSFDDAYFTFDNSYIESVWSI
                     IAEVDRRKYLYKDYKVLPWCSRCGTGLSSHELAQPGAYQDIKDLSIYAKFKIVGFDKA
                     YFVAWTTTPWTLPGNLALAVGKDITYVEAKVGEEILVLAKDRLSVLTESYEIIAEHKG
                     SEMVGMEYEPLYPFIREKGGEGMENAFKVYTADFVTTEDGTGIVHTAVMYGQEDFDLG
                     TKIGLPKVHLVSPDSTFVKGTGWLEGRSVVDEVLAVDILKDLQSKGLVYKKESYLHSY
                     PFCWRCKTKLIYYARDSWYIRMSSLRGELLSENEKINWEPDYIKNGRFGEWLREVKDW
                     AISRERYWGTPLPVWLSEGGERIVADSIDTLRKYVKKSGNKYFVMRHGGSEGNEKDLV
                     SYKLEASDHLTEAGRGQAIDSGENLKKEKIDLIISSPFARTKETAEIVRSELGLAEDK
                     LIFDERLREINPGDYDGKNWDEYHRHVYNVGANWFNESIAGGESLADVCKRVGECLYE
                     IEEKYKGKNILIVTHGGPAWLSFVISGYYDPRNKKYHIPNTHIFIKEFKRFNNAEVRE
                     LPFAPLPHNEGYQVDLHKPYIDEVQLEKDGVEYKRTKEVMDVWLDSGAMPIASYAGTG
                     EEHYPADFISEAIDQTRGWFYTLHAVGVLLGKGASYKNVICLGHILDKEGKKMSKSIG
                     NVVEPFSAIEKYGVDVLRLWMYSVNQPGEPKNFDEKSVEEVNKRIFNLLDNVYSFYEL
                     YRDEEVEKTNIKESKNILDKWILAKLDLLIEDMTKNINEYRLLEPVRGMRDFIDDLST
                     WYLRRSRDRVKSGDVEVKQTLYHILKTLSKLLAPFAPFAAEELYQKLRNEVDKESVHL
                     DSWPEVKKKFSLFGSGTDKLVDDMEEVRRVVSLALEARSKANIKIRQPLQELRIKNSE
                     LSVEYLDLIKDEINVKSVVADKNLKEEVLLDTKLTPELEEEGRVREFIRNLQEMRKEK
                     NLKPSDKMEYKVAEDIDLIKKYQSTVESSTNTTLSF"
     gene            11164..11727
                     /locus_tag="PLD99_01915"
     CDS             11164..11727
                     /locus_tag="PLD99_01915"
                     /inference="COORDINATES: protein motif:HMM:NF023393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HRH25691.1"
                     /translation="MSHHIYTTKGIVLSLRPSKEVDKLALILTRDLGVVYGSVRGIRQ
                     TSSKMRGVISDLALIRVSLVKGKRSWRVTTASLIESPSSVLREEREMLKALYRVGNFL
                     SRLIRGEEKHVEIFDDFEDAIVTLISGRISKSEIEAWELLVVSKLLSHLGYLSKDVIP
                     RDLSEAKERKKELVAQINTGILESGLR"
     gene            11744..13672
                     /locus_tag="PLD99_01920"
     CDS             11744..13672
                     /locus_tag="PLD99_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25692.1"
                     /translation="MSVPQNSGKSRLERMGEKLYSRTKYHDPMDTRESINPVEAEAAP
                     SEWDSPKIDDLLARESNTQDHHPIIKKMFYISLIFSVCAALAASLIYFNGDNFISTKN
                     LDIEIDGPINIGAGEIADLEISIINKNNASLDKLTLSIDYPDGTRSATDANDGQTNVV
                     EKLEELGPGKRITKNEKAIFLGVEGQVREVKVSVEYGVKGSNATFTKEKSFEITIGSS
                     PVTVSVSKPQTVDSGDEFLTTITITSNSKEKLDNVILRGEFPYGWRFVNASPQPTNKE
                     KTLWSIGSLAPKDKKIVTLRGVLSGEDGEERTFRYFVGVGEGQATKIDTNLYSDSVGV
                     TIEKPTIDLNVKLNGETSEEYIAPAGKNILTTINFKNNLTETLENPVVEVRLSGGALD
                     KNNITVQNGGTYNATNNSIVWNRSNSGDIEDLNPGDSGNLTFEFASLEVLPLGSQNQR
                     IDLTIILTGRPEGSALDSKVTLTRSVKIASEIVLSAKSLYARGPIENKGPIPPKAETE
                     TTYTITLNLSNTRNDVDNVKVSGQLGPNVEWAEEVAPANDSGLSYNATTRTVSWEIGK
                     LLSGSGFSSPGKEVSFKVSLMPSLGQVGGVPVLVSNITLTGFDSFTASDVRISHPSVT
                     TRISSDPTYVQGDEIVVK"
     gene            13683..14885
                     /locus_tag="PLD99_01925"
     CDS             13683..14885
                     /locus_tag="PLD99_01925"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRH25693.1"
                     /translation="MEKLISLAKRRGFIYQGSEIYGGLAGTWDYGPLGVLLKENVKNL
                     WLKMFRSEREDVYGLDASIIMNPKVWEASGHVAGFADPMENGEQFNVMFKTSVGAGEE
                     ATVSYLRPETAQGIFVNFKNVVDSFHPKLPFGIAQVGKAFRNEISPRDFIFRQREFEQ
                     MEVEYFVKESEWEKHFEMWRKTMHQFGERVGISHVHELDVPDNDRAHYSKKTIDFEFD
                     YPFGRKELWGLAYRTDYDLRQHSTSSGQALEYFDEENGERFIPHVIEPSLGVDRTILA
                     ILTSAYTEDDLGGETRSYLKLPKEIAPIRAAVFPLLKNKPELVEKAREVYKMLKSEFG
                     NIEFDDNGNIGKRYRRQDEVGTPYCITIDFDTLGETKELEGTVTVRNRDTGEQSRVKI
                     EDLKEHLR"
     gene            14903..16171
                     /locus_tag="PLD99_01930"
     CDS             14903..16171
                     /locus_tag="PLD99_01930"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRH25694.1"
                     /translation="MQYKKITLPNGLRIIYVPVKGNPSVTVMALVETGSNYESKEENG
                     LSHFLEHMVFKGTTNRPKSSDITRELDGLGAQSNAFTGNEMTGYYAKAEKKHFKKLLE
                     IIADLYLNPTLPKEELEKERGVILQEISMYEDLPQRKVWNVFAELLYGDTPAGRTILG
                     PEKNIKKFKREDFEAYRAKHYTAKKTLVVVAGDVKESDLVKEVEKHFKDIRDAKKLGK
                     EKVSSLQDSPQLKVFKKKTDQTHLILGFKTKPKSEKERAVLELLAGVLGKGMSSRLFE
                     RLREKMGVCYYVRASDNEHTDHGYFAISTGIDGKRVEEVVSALLEECKKLTTETVTEL
                     ELNKVKDYLVGNMYMGLETTDSLVEFYADQEITREKLFEPKYFEESLRKISGKDVLKV
                     AQELFKKERLNLAVVGEMKNPSRLKKLLVL"
BASE COUNT         4610 a         3236 c         4240 g         4117 t
ORIGIN      
        1 ctattcttac ctcagttcta cttgggttct tcgactacct actctcactc gttattcaga
       61 agttcgttat ctagttccac aagctcacta taaataaaaa ctatgtcaaa gcaaaaaatt
      121 tcagaaggtc gaaactggta tgtcatccac acttatgcgg gttatgagaa cgcagtggag
      181 aggaacctga agcaacgaat tgagtcactt ggtatggagg acaagatttt caatgtagtt
      241 gtacctaccg agaagaagat caagatcaag ggtggcaagc gtgttgagga agaggagaag
      301 atttatccag gttacatctt ggtagacatg atagtgacag acgattcttg gtatgtggtt
      361 aggaatactc caagagttac tggtttcgtt ggctcgggtg tgtacccagt gccaatagac
      421 aagtccgaag ttgattcgct cttcgccagg atgaacaccg acaaggtaat gcacaacatc
      481 aacctagtac ctgaggatgg agtgctcatc gtggacggcc cattcaagga ccttgagggc
      541 aaggtttctg agattgatca ggagagaggc aaggttaagg tgctggtgtc gatgttcggt
      601 cgtgagaccc cagtcgagct cgacttcttg caggtgaaga agatgtaagg tagtctaaga
      661 gtcccaaata tatggcaaag aaagttacaa aaatattaaa gcttcagatc ccaggtggta
      721 aggctgttcc aggacaggct cttggaccag tccttggagg tgctggtatc aacatcggtg
      781 agttcgttaa gcgctttaac gacgagacca aggaccgagt gggcgacata gtccctacag
      841 tggttgaggt gtacgacgac cgtacctacg gcatgactta taagaccgag ccagcttcaa
      901 acctcatcct taaggtccta ggcaaggaga agggctcagg caagccaaat acagccaagg
      961 ttggtactat caccaaggcc caggtaaagg caatcgctga gaagaagatg gccgacctca
     1021 atgctacaga tatagatgca gcaatgaaga tcatcgaggg ctcagcccgc tcaatggggg
     1081 tggatgttaa ataactacaa atattaagcc acaaagtccc gctctgcggg acttttgctt
     1141 ttgaccgaag actagagtac tcttcgttaa gatttgaacc cttaaacgag cggggaatta
     1201 atgcgcaaga acaagctctt tgccgtgatt catgttgagg acgaaaagca ggctcaccga
     1261 aatgccgttg tcgcgctcaa taatggtgca gacggcatat tccttatcaa ccaccgcgtt
     1321 tctgtgcttg aattgatccg atcttacaga tatgtgaggg agaggcacaa agcttcttgg
     1381 atcggtttga acttgcttgg attcagcttg actcaggcag tccacaacaa gcgcctagga
     1441 gattctctcc cgagagatat ccaggcactt tggtgtgatg atgttcgcta tactgaaggc
     1501 ctgggtcctg agagagctgt ttactttgca gatcgtctcc atttcgaatt gcaggtggcc
     1561 acgaaccacg ccctgctttt tgggggttac gccttcaagt atcaagagcc tgtgagtgat
     1621 ctcgttcttg gggcaaggct cctcgcccca cacgtcggag ttatcaccac aagtgggccc
     1681 gagacgggca ctcctccaac tacggagaag atcgagttga ttcgtaaagg tatgggggat
     1741 ggtgccaagc ttgcagtagc gagcgggatg actccggaga atgttgaccc attcctcgac
     1801 ctcgtggact acttcttggt tgcgaccggg atcagcgagt ccttcaccga gctcgatcct
     1861 gtgagagtga gattgatggc cgacaaaatg cgccgctagg gttaccccta gcggtttttc
     1921 ttttgacatt atgcttattc cagtgcttat ttttaagtat tctcgctttt ttaacttata
     1981 acagtaaagt taacgtagat tgatcattgt catctacaac aagaggacca aatgacagac
     2041 aataaggagt tggcagccag gcttggtcag atagtgatcc atgctcgaaa ggaaacagac
     2101 atagatacaa ggagtcgttt tcttcagaat gatgatgagg tcaggttcct tgttgatcga
     2161 gcgagaaatc ttggtcttcc ggtagttctg acacagggca catttgatct tgtccatatt
     2221 ggccacgctc gttatgtgag agaggctaag aatcgtggac atcttcttat cgttggaatt
     2281 gatgatgatg agaaggctcg cgagagaaag ggggagaatc gtccagttgt accattcatc
     2341 gagcgttgcg agatgatgtg tcatttgaga tatgttgatg ctgttgctat gaagaagcac
     2401 gatgatccta agtggcacat gatcaagttg gtgagaccag atgttcttgt cgccgttgag
     2461 ggtacttaca gcgaggaaga gaagatggag ctcgaaaaga tctgtgacca agttgtcgtc
     2521 ttgcctcgtc aagcggaaac aagtaccagt gcgaaggttc gtactctcgt cctagacggc
     2581 gctgagaatc tgaccagaat cctggcacag aaacttcctg acttcatcca caggatctat
     2641 caggatctga agaagaatgg tggagcatga gaaaggcctt ggtcgcttat gttccagtgc
     2701 ttcatcaggg gtatctagat ttcttcaagc gtcacaagga cgctgaagaa ttgttcattt
     2761 tcagccagcc aatcatagat aaatttgatt ggttgaagag aaaggacatc cgggcagtta
     2821 acccagaaga aatgcttctt gcgctccgtg ctttaggaat tgcgaagcaa gtgaccttgc
     2881 tcgatgatga acaaatcgcc aagcttcgtg atagcaatat ctctcttgtc tttgctgacg
     2941 agagcgagtc tcgacgagtt gtcgaagagt tctttggcga atcgcaggtc agctttgata
     3001 gtgtgttctt aaggtgggac aagcccagag tccttgatga gaagaatgca cagggatttc
     3061 cagtgacttc ggaagaagtg catcggaagt ttatggacaa agcctcactc gcctcggaaa
     3121 gaagttctga ttggtggatt catgttgggg ctgtactggt gtccggggat cgggtggtgg
     3181 ttggctataa caggcacaca ccatccgaac atacccccta cgctctaggt gatccacgca
     3241 gtctgttcaa taaggggata gccgtggagc tgacaagcgc cttgcatgct gagggagcaa
     3301 ttgtgggcgc tgcattacga gagggtgtgc agaccaaggg cgcttcgctc tacatcactc
     3361 attttccatg tccgtactgt gcaaatatct tggccgagac tggtatcgcg gagatttatt
     3421 ttcgcaccgg ttactcgatg cttgatgggg cggaccttct taggaaggcg ggcatcaaac
     3481 tcttccaagt tgtagatgaa aaatcccccg agtagtactc gggggattta ctttcttgcg
     3541 taacccaatt tcttctttat gtcttgtgtg ttcttacctt cccttactcc gacgacgata
     3601 tgggcatgaa gatgagcgat agtggcaccg ttataacgca tgtcaccgaa cctcatgagg
     3661 aacgtccctc ctgggattct gaatcgcttc ttggcccact tcaacacggg agagagagcg
     3721 gcaagcgagg catgtgtcat gccagacaaa tcctcgatat gcctcttgtg cacaaggagt
     3781 agatgcaacc tcgtgccctc gtacgggtaa tcgtttttcg tcaggagcca gtgatgattc
     3841 tcaaagagga ttggtgcctt atggtctgct tcgaaattct ctcggcagaa tgggcaaatt
     3901 cctcttcgtc tgatgtcttc catctgagcg cgttgctctg gaactctcgc gttcgtgata
     3961 tgcatcaacc ttttcatgtt gctctccgag agtagatctc ccaagtgaag ggaaactcgt
     4021 gcttctcatc cactgggtgg tcgtccgaat actcgagtat ccactcgcct ttgttccact
     4081 ccgggaaaac tgtgtcgcct tcgatctcag tatgcacttg ggtgaggtat atcttgctcg
     4141 cgaacgggag cgctagtctg aatagatcag caccaccaat cacgaacaca cctctcgttt
     4201 tcgacatttc aactaccata tcgaagttgt cggtgaagat cactccatca caccttcgtg
     4261 ctcgtggtct tcgagtaagt acgacactct ctcttcctgg caatggtttg ccaattgcct
     4321 tgatgattga gtcgaaagtt tttgtaccca tgaccacagg ataccctgcg gtcattccct
     4381 taaaccacct gagatcatcg ggtagatgcc agggcaatcc accgttcttt ccaatgaccc
     4441 cgttttgaga tattgccaca atgatattga gcattgtttc tcctatacgg cgacggggat
     4501 ttctcggata ccaggatgtg gatcgtaatc cgagatcacg aaatcatcag aacggatagc
     4561 gaaaatatct ttcttatccg tattgagtgt catcgtgggg aagcgacgtg gttctctgga
     4621 aagcatctct ttcacttgct ccacctgatc cacatagatg tgggcatcag agaagctgtg
     4681 aatgaatttg tacggcttgt gccctgtcac atgggccaga gccagcgtga aggctgcgta
     4741 ttggatcatg ttggctggca caccgacagg gaagtctgct gaacgttgga acatgtgaag
     4801 taccaacccc ttgtgtggga tgatgcgcac atgcatccag ccatggcatg gagccacgac
     4861 tactcgttgc ttctttccct tgcctcgacc gacatattgc ggaacccatg gcgacacgaa
     4921 gtgtgtgcgg agttggggct tttccgagat ctgttcaacg agatgtttga tctgattgaa
     4981 aggatttcct tcggctgtgg ggaagtcgtg catggcagca tacccgcctg gtcctaggtc
     5041 gccggcttcg agtccgcgtt tggcacactt ttctgccgtc aaccacttgt cccaccagct
     5101 acacccaaat tcttcaagac ctttctgagt ccttacgccg tttgcgaaag ccaagagctc
     5161 tccaaccgcg gccttccaga acgggatttt cctctcagtg atgagtggaa ccccgttgct
     5221 cagatcgaaa tgcatcggag gtggggccat gtaggtcagg gctccgacac cttgctgggt
     5281 ttctccgatc aattctcctt gttctagaat gagacggagt cgatcacgat attgtgtatc
     5341 cacggtgcga tcagaaaacg gaagcatctg tctatccttt tgttgggttg tgaaagggcg
     5401 acttctgacc cccatactac tcattttgat agaggtgtct attcgggtat cattgacctt
     5461 atgaaggaga ctattcaggt actaataagg aaggcactga aggaacttgc tatagaggac
     5521 aagggtttta tcgtagaaca tccaacagat cttagaatgg gtgattactc gacgaacgtt
     5581 gctataaaga atggtctaaa gaagcaagaa atatttgagt atataaagag ccataagcca
     5641 gaagagattg agagggtaga gatggtaggg ccggggttca ttaactttta tttgtctaag
     5701 aagttttttg ccgaatcgtt gaagcagatt atcaaggcag gagacaagtt tggtcgaacc
     5761 aagaagttta agggtgagaa ggtgatggtc gagtacacag acccaaatcc tttcaaagca
     5821 tttcacatag gacacctcat gagtaacgcc attggtgagg cggtgtctcg agttatagag
     5881 tggaatggtg cagatgttag gcgtgccaac tatcaaggtg atacaggtat gcacatagca
     5941 atggctgtgt ggggttactt ccacggagag tggtcgactg cctatgctat aggcgccaag
     6001 gcctatgtag acaatttaga ggcaaggaag gagattgagg aaataaacaa aaaaatattt
     6061 gagaggagcg acaagagtat caccaaggtc tatgacgaag gcagaaaggc gagcctagaa
     6121 tacttcgaaa gtatttataa aaagcttgga actaagttcg actattactt ctttgagtca
     6181 gaggcgggag agctcggtaa gaaattggta gagaagaatg ttggcaagat tttcgaagag
     6241 tcagacggtg caatagtttt tcatggagag aagttcgatc cctctctcca caccagagtg
     6301 tttgtgaaca gacacaagct tccaacctac gaagccaagg acttgggcaa tgcggtgaac
     6361 aaatataaga aatataaata caccaagtca gtagtaataa ctgggaacga gatcaaggaa
     6421 tacttcaagg ttatgctcac cgcactctct caaatgtacc cagatctttc caagagtacc
     6481 aaacacttgc cacacgggat gttaaggcta ccaacaggca agatgagttc gcgtacagga
     6541 gatgtcatca ccgcagaaga tttgatcaaa gatgtgagtg cgaaggttaa agatagtgag
     6601 ccactttcaa tcgctgctat taagtacatg attctcagac agtcgatagg taacgacata
     6661 gtatttgatt tcgacaaatc ggtttctacc gagggagact ctggtgtgta tctcgagtac
     6721 gcacacgcga gggcgacgtc tgtgttggat aaggcgacga ctagttggag cattaagggc
     6781 gagagaggag agacggccga gatagagaga cttttgtacc gcttgccaga gatagtcgag
     6841 agagcaggag aggattactc gccacagttc gtcactactt accttacaga acttgcatct
     6901 tcatttaata acttttacgc gcatgagcag gtcaacagcg attcacctga gacgaagtat
     6961 agactggcaa ttgtagaggc cttcaaggtg gtgatgaaaa acggtttgac catcttaggc
     7021 atccccgtac tagatagagt gtagacagag agttttttag tgtgtagagt gtcggaagaa
     7081 gcatccttca aggagatcaa atgcctggtg atgttgttgc attttcggga ccgactgctg
     7141 tcggcaagaa caggatcttg gacaggttct gggctacgaa ggattctgcg aagcgggttg
     7201 ctcatacaac tcgtcagccg agagttggag aggtgaatgg agataattac cacttcgtta
     7261 cccgcgagga atttgaggtc ctcgtagctc aaggcgagtt ccttgagcat gcagaagtat
     7321 acgggaatct ctatggcacc acgaggctcg gcctaagcga ggttgtagca tccggtaaga
     7381 atgtcttctt tgacctcgac attcaggggg ctcgtcagct caaggctaag atgccggaag
     7441 tcatatcgat ctttgttctg ccaccaagca tcgctgaact cgagcggagg ctaagggatc
     7501 gtaagaccga agacgaggag accatacttc ttcgattgaa gaaagtcgaa ggagagatcc
     7561 gacaagctcc aaggttcgat tacttcgtgg tgaatgacga tctcgataag gtggtacacg
     7621 agatccaagt acttctcgga attctctgcc ttggtaagag gcctcctccg gagagattca
     7681 ggaacacaga tcacatcgac aagcttctgc tggagttagt ggcttcccaa taggggagcc
     7741 tttttctttg tcataattta agctagaatg tctccacaat gcccgaagat aaagggatga
     7801 ctcgctccga aagagaagag aaggtcctac aattttggaa ggagaataag atattcgaga
     7861 agtcactcga gaagccttcg cctaagggcg aattcgtgtt ttatgagggg cctcctacag
     7921 ccaatggtaa gccggggatt catcacctag agagtagagc cttcaaggac gctgtgccta
     7981 ggtacaagac catgcagggc tataaggtgc caaggaaggc aggttgggac actcacggtc
     8041 tacctgttga gatcgagtca gagaagaagc tcgggctcaa gagcaagagg gagattgagg
     8101 agtttggtgt ggcgctattt aatgccgagt gtaggaggag tgtgctttct tatatagatc
     8161 tctggtccaa gttcaccgag cgtattggtt attgggtaag ttttgatgac gcgtacttta
     8221 ccttcgacaa ctcatatata gaatctgtgt ggagcattat cgccgaagta gataggagaa
     8281 agtatttata caaagattac aaagttttac cttggtgctc acgttgcggt acaggacttt
     8341 cttctcatga gctcgcacaa cctggtgcgt accaagatat taaagacctt tctatctatg
     8401 cgaagtttaa gatagttggt ttcgacaaag catacttcgt tgcgtggaca actactccat
     8461 ggactctgcc aggcaactta gctctagctg ttgggaagga cattacttat gtcgaggcaa
     8521 aagtaggaga agaaattcta gttttagcta aagacaggct ttcagtactt acagagtctt
     8581 atgagatcat cgccgagcac aagggtagcg agatggttgg tatggagtac gaaccacttt
     8641 atccctttat aagagagaag ggtggagagg gtatggagaa tgcgtttaag gtttatactg
     8701 ctgacttcgt taccacagag gatggtacag gtattgttca tactgcagtc atgtacggtc
     8761 aggaagactt cgacttaggg accaagattg gactacctaa ggtgcatctt gtatctccgg
     8821 attcaacctt tgttaaaggt actggttggc tcgaaggcag gtcggtagta gatgaagtgt
     8881 tggcagtaga cattttgaag gatcttcaat ccaaaggact ggtatataag aaagagagtt
     8941 acttacattc atatccattt tgttggagat gtaagaccaa actcatttat tatgcacgag
     9001 attcttggta tatcaggatg tcgtcactaa gaggagagct attgagtgag aacgagaaaa
     9061 taaattggga gccagattat attaagaatg gtaggttcgg cgagtggctc cgagaggtta
     9121 aggattgggc aatttctcgc gagaggtatt ggggcacacc actgccagta tggttaagtg
     9181 agggcggaga gagaattgtt gctgattcta tagacacttt gagaaagtat gtgaagaagt
     9241 ctggtaacaa atatttcgtc atgcgacatg gtgggtcaga gggaaatgag aaagatttag
     9301 taagttacaa actagaagcc agtgaccatc taacagaggc gggcagagga caggcaatag
     9361 attcagggga gaatctgaag aaagagaaga tagatctgat tatctcatct ccttttgctc
     9421 gtactaaaga aactgcagag atcgtgagga gcgagcttgg gcttgctgag gacaaactaa
     9481 tttttgatga gagactgcgt gagataaatc caggggacta tgacggtaag aattgggacg
     9541 agtatcaccg acatgtatac aatgtcggag caaattggtt taacgaaagc attgcaggtg
     9601 gagagtcact agccgatgtg tgcaagagag tgggtgagtg cttgtacgaa atagaggaga
     9661 agtataaggg caagaatatc ctcattgtta ctcacggtgg accagcctgg ctctcattcg
     9721 tcatatctgg gtactacgat cctaggaaca agaagtacca cataccgaac acacacattt
     9781 tcatcaagga gttcaagcgc tttaataatg cagaggtgcg tgaactacca ttcgcaccgc
     9841 tacctcacaa cgagggttat caagtggact tgcacaagcc ttatatagac gaggttcagc
     9901 tagagaagga cggcgtcgag tacaagagga ccaaggaagt catggatgtt tggctcgact
     9961 caggtgccat gccaatagca tcttacgcag gtactggtga ggagcattat cctgcggact
    10021 ttatatctga ggctatagac cagactaggg ggtggttcta caccctccat gctgtcggag
    10081 tactcttggg caaaggggcc tcgtataaga atgttatttg ccttggacac atcttggaca
    10141 aggagggtaa gaagatgagt aagtctatag gaaatgtggt cgaaccattt tctgctatag
    10201 agaaatacgg tgtggatgtg ctccgacttt ggatgtactc ggtgaaccag ccgggtgagc
    10261 caaagaactt tgatgagaag tcggtcgagg aggtaaacaa gaggatcttt aacttactcg
    10321 acaatgtcta ttctttctat gaactatata gagatgaaga agtagaaaaa acaaatataa
    10381 aggaatcaaa aaacatttta gacaagtgga tcctagccaa actcgacttg ctcatagagg
    10441 acatgaccaa gaatattaat gagtacagat tgctcgaacc tgtgagaggt atgcgcgact
    10501 tcatcgatga cttgtctact tggtacttac gcaggtcacg agacagagtg aagtcgggtg
    10561 atgtggaggt taagcagact ctttatcata ttttgaaaac tctttctaaa ctccttgcac
    10621 catttgcacc atttgctgct gaagaactgt atcagaagtt aaggaatgag gtggataaag
    10681 aaagtgtcca tctagacagc tggcccgagg tcaagaaaaa gttctctttg tttggatcag
    10741 gtacagataa attagttgat gatatggaag aagtaagaag agtggtgagt ctcgctcttg
    10801 aggccagatc gaaggcaaac attaagatca gacaaccact ccaagaatta agaataaaga
    10861 atagcgaatt atcggttgaa tacctagatc ttattaaaga tgagataaat gttaagagtg
    10921 ttgttgctga taaaaattta aaagaagaag tattgcttga taccaagcta acacctgaac
    10981 ttgaggaaga ggggagagtg agagaattta tccgtaatct acaagagatg aggaaagaaa
    11041 agaatctcaa accatcggac aagatggagt acaaggttgc agaagatata gatctaataa
    11101 agaagtatca atctacggta gagagttcaa caaacactac actttctttc taatggcttt
    11161 ctaatgtctc atcacatata taccaccaaa ggtattgttc tctcccttcg gccatctaag
    11221 gaagtagata aattggcact catccttaca agggatctag gagtagtcta tggatctgta
    11281 agaggtatca ggcagactag ctcgaagatg aggggggtaa taagtgatct cgcactcatc
    11341 agagtatctt tggtcaaggg caagaggagt tggagggtga ccacagcaag cttgatagag
    11401 tcaccgagta gtgtgcttag agaagagcga gagatgttga aggcgctata tagagtagga
    11461 aattttctat ctagacttat acgaggtgag gagaagcatg tagagatctt tgatgatttt
    11521 gaggacgcaa tcgtgacctt aataagtggg agaatatcta aatcagagat agaggcttgg
    11581 gagctcctag tggtgtcgaa gttgctctcg catctgggtt acttatctaa ggatgtaata
    11641 ccaagggatt tgagcgaggc aaaagaaagg aagaaggaat tagtggccca gattaacacc
    11701 ggcatactcg agagcgggct caggtagggt ataataacca cacatgtcag tcccccaaaa
    11761 tagtggcaaa agtcgcttag agcgtatggg tgagaagctc tactcgcgta ctaagtatca
    11821 cgatcccatg gacaccaggg agtctattaa ccctgtcgaa gctgaagctg ctccgagcga
    11881 gtgggactct cctaagatcg atgacctttt ggccagggag tccaacaccc aggaccatca
    11941 cccaataatc aagaagatgt tttacatctc tctcattttc tctgtttgtg ctgcgcttgc
    12001 tgcgagtttg atctacttta acggtgataa cttcatttct acaaagaatt tggacatcga
    12061 gatagacggt cctataaaca taggagcagg ggaaatagct gacctggaga tcagtattat
    12121 taacaagaac aatgcatcac tcgacaaact tactcttagt attgattatc cagatgggac
    12181 taggagtgcg acagatgcca atgatggcca gacgaatgtt gtagagaagt tagaagaact
    12241 tggtccaggc aagagaataa ccaagaatga gaaagcgata ttccttgggg tcgaaggtca
    12301 ggtgagagaa gtgaaggtct ctgttgagta cggtgttaag ggctcgaacg ccaccttcac
    12361 caaagaaaag agtttcgaga ttactatcgg ctcttctcca gtaactgttt cggtttccaa
    12421 gccgcaaaca gtagattctg gcgacgagtt cctcactaca atcaccataa cctctaactc
    12481 gaaggagaag ttagacaacg ttatcctccg tggtgagttc ccttatggat ggagatttgt
    12541 taacgcgagc ccacaaccga caaataagga gaagaccttg tggtccatag gtagtctagc
    12601 cccaaaggac aagaaaattg tgactctaag aggtgtgctg agtggtgaag atggagagga
    12661 gagaacattt agatacttcg ttggagtagg cgagggtcag gcaacgaaga ttgatacaaa
    12721 cctgtactcc gactcagttg gtgtcactat cgagaagcca acaatcgatc ttaatgtgaa
    12781 gcttaatgga gagacaagtg aggagtacat cgcacctgca ggaaaaaaca tcctgaccac
    12841 tataaatttc aaaaacaatc tgacggaaac tttggagaac ccggttgtcg aggtgcgtct
    12901 ctcgggtggg gcgctagaca agaacaacat cacagtgcaa aacggtggta cttataacgc
    12961 caccaataac tcaatcgttt ggaacaggtc taactcaggt gacatcgaag atctaaatcc
    13021 tggagactct ggtaatttaa catttgaatt cgcatcactt gaggttttgc cattagggag
    13081 tcagaatcag cgtatcgacc tcactattat tctcactggt agaccagagg gctcagcctt
    13141 agacagcaag gtcaccctga ctcgctcggt caaaattgct tccgagattg tgcttagtgc
    13201 caaatctctc tatgctcgtg gtcccataga gaacaagggt ccaataccac caaaggctga
    13261 gacagagact acttacacta ttactcttaa tctctcgaat accaggaacg atgtagacaa
    13321 tgtgaaggtc tctggtcagt tgggaccaaa tgtcgagtgg gccgaggagg tggcacctgc
    13381 gaatgactca ggtctctcgt acaacgcgac aacaagaaca gtttcttggg agataggcaa
    13441 attgctgtcc gggtctggct tctcaagtcc gggtaaggag gtgtcgttca aggtctcgct
    13501 tatgccatct ttgggacagg tgggcggtgt accggtactt gtaagcaata ttactctcac
    13561 cggctttgat agctttaccg cgagtgacgt gaggatttca cacccttcgg taacgactag
    13621 aatttctagt gacccaacct atgtgcaggg tgacgaaatt gttgtaaagt aggcgaataa
    13681 ttatggaaaa actgatatca ctagccaaga ggagggggtt catctaccag gggtccgaga
    13741 tctacggtgg tcttgcaggt acttgggatt acggtcctct tggagtgctt ctaaaagaga
    13801 atgtaaagaa cctatggctc aagatgtttc gaagtgagag agaagatgtc tatggtctag
    13861 acgcatctat tatcatgaac ccaaaagttt gggaggcctc gggtcatgtc gctggctttg
    13921 ccgatccgat ggagaatgga gagcagttca atgttatgtt taagacctct gttggtgcag
    13981 gagaggaagc aactgtttct tatctacgac ccgagacagc gcaggggata tttgtaaact
    14041 tcaagaatgt tgtggattca ttccacccca aactaccttt cggtattgct caggttggta
    14101 aggcttttag aaacgagatc tcacctagag acttcatttt ccgtcagcgt gagtttgagc
    14161 agatggaggt tgagtacttt gttaaggaga gtgagtggga aaaacatttt gaaatgtggc
    14221 gcaagactat gcatcaattt ggcgagagag tgggtatctc acatgtgcac gaacttgatg
    14281 tacctgataa cgacagagcg cattactcga agaagaccat cgacttcgag tttgattatc
    14341 cgtttggtag gaaggagctt tggggtcttg catacaggac tgactacgac ttaagacaac
    14401 attcaacctc atcaggtcag gcgcttgaat actttgacga ggagaacgga gagagattca
    14461 tccctcatgt tatagaaccg tccctcggag tggatcgcac catacttgcc attcttacat
    14521 ctgcatacac cgaagacgat cttggtgggg agacaagaag ctatttgaaa ttgcctaaag
    14581 agatcgcgcc catccgtgca gccgtcttcc cacttcttaa gaacaagcca gagttggtag
    14641 agaaggcgag agaggtttat aaaatgctta agtctgagtt cggtaatatt gagtttgatg
    14701 acaacggcaa tattggtaag cgttatagaa gacaggatga ggttggtact ccgtactgta
    14761 ttacaatcga ctttgataca ttgggtgaga ccaaggagct agaaggtacg gtaactgtta
    14821 gaaacagaga caccggcgag caaagtaggg taaagattga ggatttgaaa gaacatctca
    14881 ggtaaggtac gcttgaggct ctatgcaata caaaaagatt actctgccga acggtttgag
    14941 aattatttat gtcccagtta aggggaatcc ctcggtcaca gtgatggcct tagttgagac
    15001 gggttctaac tatgagagta aggaggagaa cggactttca cacttcttag agcatatggt
    15061 tttcaaggga acaacgaaca gacccaagtc tagcgacatc actcgcgagc tcgatggtct
    15121 tggcgcacag tcaaacgcat tcactggtaa cgagatgact ggttactatg ctaaggcgga
    15181 gaagaagcat tttaaaaaac ttttagaaat tattgcagac ttatatttga acccaactct
    15241 tcctaaggag gagctggaga aggaaagagg agtcattctt caagagatct cgatgtatga
    15301 agacctacca cagaggaagg tatggaatgt tttcgcagaa ctgttgtacg gtgacactcc
    15361 agccggtaga acaatccttg gcccagagaa aaatatcaag aagtttaaga gagaggattt
    15421 tgaagcctat agagccaagc actacactgc aaagaagacc ttggtggttg tggcggggga
    15481 tgttaaggag agtgatctcg tcaaagaggt tgagaagcat tttaaggata ttagggatgc
    15541 aaaaaagttg ggtaaagaga aagtttcttc actccaagat tctccacagc ttaaggtttt
    15601 caaaaagaaa acagatcaaa ctcatttaat cttaggattt aagactaaac caaagagcga
    15661 gaaggagaga gctgtgcttg aactactcgc tggggtgctt ggtaagggta tgtcctctag
    15721 acttttcgag agactaagag agaagatggg tgtctgttac tatgttcgtg catctgacaa
    15781 cgagcacaca gatcacggtt acttcgctat atctacaggt attgatggga agcgggtaga
    15841 agaagttgtc tcggcccttc tcgaggagtg taaaaagcta actacagaaa cagtaactga
    15901 attggaatta aacaaagtta aggattatct agtgggcaac atgtacatgg gtcttgagac
    15961 cacagactca cttgttgagt tttatgccga tcaagaaatt actcgtgaga aactctttga
    16021 gccaaaatat tttgaagagt cgttaagaaa gataagtggc aaagatgttt tgaaggtagc
    16081 tcaggagctt ttcaagaaag aacgacttaa tttggctgtg gtaggggaga tgaagaaccc
    16141 ttctcgcttg aagaaactct tggtgttatg attgtcccat ggatcaatct aatggcaata
    16201 tag
//
LOCUS       DAORIT010000015        16091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_609_length_16091_cov_51.226615, whole genome shotgun sequence.
ACCESSION   DAORIT010000015 DAORIT010000000
VERSION     DAORIT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16091
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_609_length_16091_cov_51.226615"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..125)
                     /locus_tag="PLD99_01935"
     tRNA            complement(50..125)
                     /locus_tag="PLD99_01935"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Asn,seq:gtt)
     gene            180..644
                     /locus_tag="PLD99_01940"
     CDS             180..644
                     /locus_tag="PLD99_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRH25695.1"
                     /translation="MKSFRDYVHARVSKQSLHLLLIAGVISLFTTGFALTKQVEIALD
                     EANAGIAPSLHLAYVNRDANNPRIPNTPPPYLEVAKKTLKSGDAGEEVLLLQSFLSWR
                     GYWPKGEPLSKYFGETTKKAVMNYQMKNGLPPEGTVGPLTREVWRKDIERAL"
     gene            696..1061
                     /locus_tag="PLD99_01945"
     CDS             696..1061
                     /locus_tag="PLD99_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HRH25696.1"
                     /translation="MSLTPDSAFEVSRPWGKFLQFTKDTPSTVKILNINAGEAFSLQY
                     HNKRDEMWFVLKGEGTIEINGESFPVLPNAKFLIPKGSTHRITAGESNVEVLEISTGE
                     FDEEDIVRLDDRYGRATNE"
     gene            1054..2157
                     /locus_tag="PLD99_01950"
     CDS             1054..2157
                     /locus_tag="PLD99_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25697.1"
                     /translation="MSDIILNQILIVCIATFLLLLLWLLSKPVLKLVHYSLNRVTHNP
                     VLAPHGLNEWETEAVFNPGAFVDDEGNIHLLYRAMGGDGLSKIGHAWSSDGKNFERKY
                     PYPVYHPQRGFGLPEDVLVPKKFDPVNYASGGGWGGCEDPRTVVIDGRVFMTYTAFEG
                     WNNARITLTSIDMQDAKSGRWKWQRPIYLSPANEMHKNWVLFPEKINGKYAILNSLAP
                     KVLIEYVDDVMQDKNIKSSHNHGGYGYTELTRKNMWDERMKGVGSPPIKTSLGWLVLY
                     HAVEPNVGYRLGAMILDHNDPTKILYRSPAPILTPDMHYENDWKPGVVYASGAVVVNG
                     ELQVYYGGGDKYICMAHTNLEQLLLWLKEYGKI"
     gene            2175..4073
                     /locus_tag="PLD99_01955"
     CDS             2175..4073
                     /locus_tag="PLD99_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25698.1"
                     /translation="MFTIKRSEDNPYLSPRKEHPWEAAGAYNWCPVKSGKTTYVVYRA
                     LSERELLEEPKIHKSVIGRATSTNGKDFKDRVPFIVPEHEWEKYGCEDPRITKIGGKY
                     YIFYTALSNYPFDASGIKVALAISKDMKTIESKHLVTPFNAKAMALFPEKINGKYAAV
                     LTFHTDMPGTNVALALFDKIEDIWSESYWKDWYEHRDRHVIDLHRSNDDQVEVGAVPV
                     KTKEGWLLIYSHANHYFSRNEHSFGVEAVILDKNNPRLIIGRTKGAFLVPELYYEAIG
                     LVPNIVFPSGALIKGKNLEIYYGGADTHSCKATVKLDDLLKSILHSSKPLVNRFVGNP
                     ILTPRLDKEWEAHGTFNPAAIDLGGMVHILYRAMSTDDTSTFGYAASKDGLVISERSD
                     MPVYTPRADFENKHQQGNSGCEDPRLMEIGKEIFMTYTAYDGYVPRVAITRILKTEFL
                     KKSWDKWSMPIIITPMDVTNKDACIIPEKTAAGYIFLHRINESICADILSSLDFEKQK
                     VVRCIELIQPRRGMWDGKKVGISGPPVKTPKGWLLFYHGVSETSTYRMGAVLLDLKDP
                     TTILARTAIPLFEPKEDYETKGIVPKVVFPCGQVVRKDTIYLYYGGADTVVGVATLSL
                     KALLKILS"
     gene            4070..4837
                     /locus_tag="PLD99_01960"
     CDS             4070..4837
                     /locus_tag="PLD99_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRH25699.1"
                     /translation="MSVEQYKNKEVFIFDLDGTLAVSKQPLDDEMHDLLDSLLDKHKV
                     IIISGGHLKQFDKQVIKHLDLTPERIPNLFIMPGSGSSMYRFNGSREEKIYADLLSPE
                     DKEKIYSAFYYALERAPFNLPEFPFGERIEDRDVEITFSALGQLAPVKEKEKWDPDHR
                     KREILIRFLLEKIPEYEIHMGGMTSIDVTQKGIDKAYGIRKASKELNIPIERMVFVGD
                     ALYEGGNDEPAKKTGIECIAVKDVDETKKLIREIISI"
     gene            4834..5376
                     /locus_tag="PLD99_01965"
     CDS             4834..5376
                     /locus_tag="PLD99_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25700.1"
                     /translation="MKKFLILIIIVGVAYGLYWYKTNNSEVKKVEIAPTVSKPDIANA
                     NFLFEGEEVKLRNGKSSTEIAPDSAVTIETALSGDIVYGDINNDSKSDAVSLATQTGG
                     GSGLFVYLLAYVSGNVEYKSLDAVFLGDRISPESLKIDSNGNVLVSYLDRKASEPMSA
                     EPTQLVKKTFKIVSGRLEER"
     gene            5400..7190
                     /gene="typA"
                     /locus_tag="PLD99_01970"
     CDS             5400..7190
                     /gene="typA"
                     /locus_tag="PLD99_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRH25701.1"
                     /translation="MQIRNIAIIAHVDHGKTTLTDALLRQNGMFAEGESMDSNALEKE
                     RGITIYSKNTSLFYKDTKINIVDTPGHSDFGSEVERVLRSIDTVLLVVDAQEGPMPQT
                     RFVLKKSLELGLKPIVVINKIDKPAARPEVVHEMVLELFLELGADNTQIDFPTVYAIG
                     REGMAKRNLGDTSDNLNPLLDVVLEHVPPAEQKLDVPVRLQAFNLAYDNFLGRLAIAR
                     VYEGVLKMGQEVWVQKNNGEKYKGKITKLFTFSGIKRVESEEAIAGDIVMLAGLPEIY
                     IGETISGDQNAEVLPFIHVDEPTISLRFLVNDSPFAGKEGKFVTGRQIRERLERELEV
                     NVGLKVDFSSSEYLTVYGRGELHIAILCENMRREGFELQVSQPHVIIKEEGGKKTEPF
                     EEVVIDVPEEASGTVISTLTSRRAQLMDMKTDNGQTRLIFEAPTRGLLGYRGQFVVDT
                     KGLGIFSSRFIEFRAYAGVIDHHDVGSMISMENGKALAFSLDNLQQRGVLYIEPATEI
                     YEGQVIGNTSKGEDMYVNPTKGKQLTNMRASGSDDAIFLVPPYKLDIERAMEIMKDDE
                     YIEITPTSVRLRKQLLTQTARAKATRKDLK"
     gene            7284..7709
                     /locus_tag="PLD99_01975"
     CDS             7284..7709
                     /locus_tag="PLD99_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25702.1"
                     /translation="MFDLTETEKQALRTNLLTSGVSGMVIGLILGFMFGIMAGIVDGL
                     MVGLKIGVMAGLMISSLIGLISVMIGGPIRATNSLTFFLGFTSVFAFFVALGHYGATK
                     AFGSLFGFLALQIVGIIFVLLIFTSLFADKRSGATTKGF"
     gene            7887..>8091
                     /locus_tag="PLD99_01980"
     CDS             7887..>8091
                     /locus_tag="PLD99_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25703.1"
                     /translation="MASRNLKDWLYTIGVLTAVMFMGWAIINIPMKDQYKDTVVSTAE
                     ASTISPRAEPVIYFSDMAGSLEQP"
     assembly_gap    8092..8191
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8192..8569
                     /locus_tag="PLD99_01985"
     CDS             <8192..8569
                     /locus_tag="PLD99_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25704.1"
                     /translation="RAEPVIYFSDMAGSLEQPSPNTISVATPSVHFVVTRTEKSSQLE
                     EGILLNLVRTTPGVDVYGSSMDADEASLVVVGKPLNLRRNVIIMSHYRFLIMVKDQGS
                     RVLQCNVEVPMHRLSNGMIVICS"
     gene            complement(8631..9362)
                     /locus_tag="PLD99_01990"
     CDS             complement(8631..9362)
                     /locus_tag="PLD99_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25705.1"
                     /translation="MSSEVIKLYKNLGETPLECIERFRAKNKGYEETNMTYLGRLDPM
                     AEGVMLVLSGNTKDKDKYLSLDKTYEFEVLWGFETDTYDILGLVTNTGAMPAKLDFKM
                     EKLLIEMREKKTQKYPIFSSKVFNKDFMKVRGKTSDLETPERGIKIFSIEHINTKIEK
                     QEDILKEILDKIDLVKGDFRQTEIKDKWREVIIPGEKVLISHFKASVSSGTYIRSLAH
                     EMGQKLGTSALAYSIKRTRVGEYKI"
     gene            9667..10431
                     /locus_tag="PLD99_01995"
     CDS             9667..10431
                     /locus_tag="PLD99_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRH25706.1"
                     /translation="MSKLDTAPYKGVRDFYPEDMRIQNFIFNTWKKVVERKGYEEYNA
                     SPLEHTEIYAEKSGEEIVREQTFTFKDRGDRSVTLRPEMTPTLARMVAARRRGLKFPL
                     RWFSIPNLFRYEKPQRGRKREHYQLNADLLGVPGLDGDKEIITLLYEIMKEFGAKDSD
                     FEIRVNNCNFEDFKNLTPNMAFDETLARGQAYYTGTVFEIFDKDPENPRALAGGGRYD
                     NLLELFDVEPVPAVGFGMGDITMRDFLETHKLLPNA"
     gene            10424..>10812
                     /locus_tag="PLD99_02000"
     CDS             10424..>10812
                     /locus_tag="PLD99_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRH25707.1"
                     /translation="MRKIVFDIETRNVFSDVGKNDPTLLDISLVGVWDSETDAYTSYL
                     QEDLSLLWPILERADMLIGFNSDHFDIPLLNKYYPGDLTKLKSLDILKEIYKTYGRRM
                     KLDQIAEGTLGKRKSGNGLEAIEWWKKG"
     assembly_gap    10813..10912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11073..11531
                     /locus_tag="PLD99_02005"
     CDS             11073..11531
                     /locus_tag="PLD99_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRH25708.1"
                     /translation="MNYFFIFGAKYLFFLSVIIAAIYFFKSLDKRKLFKFGVIILPVS
                     YIFGLVARTLYYNPRPFVTENFTPLIDHVADNGFPSDHTLFVASLAVWTTLFNKKLGL
                     VLWCIALVVAISRVYVGVHHPVDVLGSIVISALVGLMLYCINKYGEQPNK"
     gene            11509..12210
                     /locus_tag="PLD99_02010"
     CDS             11509..12210
                     /locus_tag="PLD99_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRH25709.1"
                     /translation="MENNPTSNNNNVSVPLAIILAGGMIAAAVYLGGRTPLPDTNNNL
                     AKTELPDIEPISSGDKVFGNASASVVVVEYSDLECPFCKVFHSTMHQLVDEYKGKVAW
                     VYRHYPIKELHSRATKEAEASECAYEQGGSTIFWKYIDKVFATTGTNDTLDPLELPKI
                     AGSLGLNTDVFNTCLNTGKYAAKVQSDIKKAVEAGARGTPYSVILKDGVEVETINGAE
                     PIEDVREKLDTALGN"
     gene            complement(12207..15137)
                     /locus_tag="PLD99_02015"
     CDS             complement(12207..15137)
                     /locus_tag="PLD99_02015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRH25710.1"
                     /translation="MEENFAKAYKSLNKAQKEAVDTIEGPVMVVAGPGTGKTTVLTLR
                     IANILKQTDTPPSGILAITYTDAGVKAMRGKLRNLIGTRAHEVEMHTFHSFAKAMMNE
                     YQDHFIELSDMKQMTDVDKEMLIREIIESPKHKDLRPSGKPDAYLAAIISSISNSKRD
                     AITPEDVRKFANSEIERITNDEDSISTRGATKGKLKADAEERIEKCKKTLIFADIYEE
                     YEEGKKKLRKLDFDDLIIQLLVTLKTDNLFLRLLQERFLYILVDEHQDTNDAQNYIVS
                     MLADFFETPNVFIVGDEKQAIYRFQGASVENFLLLRKRWPEMKLISLESNYRSHQSIL
                     DSSFSIIERNYTEGEHDDLRVRLVSESAESERPIEIVSVEDTRTMESYLVDELKRITK
                     DELEATVAIITKRNRDLERVIKLLEFNSIPVSSERSVDIFHHPIGVTFFDLITYVYEN
                     DKYEYLARTLSTGMWGLSFDESIALSKALRSGKLGELKVIEHKLKEIRSEAVSSSPIE
                     FIIKLGDISGFAGLVTLDPSYVYVWKGIVSLAESLARDSEVASPNELLRLMLAYRESA
                     EMKPVKVSVGAPDLKVRALTAHGSKGLEFDYVFIPYATDEAWVGRNRGSSFVLPTKDV
                     VGGDIRDMRRLFYVALTRARKHVVVLTPLEESDGKVLTPLRFISEIESGATAVSMPRK
                     KLEIVVDRKKSKEASYDSKVLALAKELLMKNGLSVTALNHFLECPNKFIYESILKMPQ
                     APSINSARGTAMHEALSHIWINEGRESLDKNEIEKIVLSVAERSIEDTFLSMRDKELV
                     MNDIREDAPAIARELETHLKTRGTVYTEKWLEAVHNANKIETGLYIPIHGKLDTIVDT
                     GDEVQVYDYKSKQAMSVNEIKGETKNSNGDYFRQLVFYKMLVLANPKWKLKRSTQSLV
                     FIRPDKKERCPIITLDVANADIDKVNTEIDSLALSVWSGEILKSTCGKEDCEYCSIRA
                     LLKH"
     gene            complement(15457..>16091)
                     /locus_tag="PLD99_02020"
     CDS             complement(15457..>16091)
                     /locus_tag="PLD99_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003145858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRH25711.1"
                     /translation="LIQEGNLGLYKAVEKFDRTKGFKFSTYATWWIRQAITRALADQS
                     RTIRVPVHMVETIAKYKQVVRRLTQDLGREPMPEEIAMEMNLDVDKIYQIEKIDQSTV
                     SLESPVGDEGDDGKSKLGDFLADDKILSPAEESSRRIISDQVKEILNDLPPKERKILE
                     MRHGLTDGYTHTLEEVGKVFGVTRERIRQIEAKAHEKIRQHEKINRLRGY"
BASE COUNT         4429 a         3709 c         3497 g         4256 t
ORIGIN      
        1 ctttttgaac ctcctaaaga atagcatatt gctatgctat tgtcaagctt ggctccgcgt
       61 ctagggctcg aacctaggac atcctcctta acagggaggc gctctaccga ctgagctaac
      121 gcggaatagg tcatatatta acagatatat ttaccttttc aatgatacaa tttatgacaa
      181 tgaaatcgtt tcgggactat gttcatgctc gagtttccaa acagtctctt cacctacttt
      241 tgattgcggg ggtgatctct ctttttacta ctgggtttgc actgactaaa caggtggaga
      301 ttgcgctcga tgaagcgaat gctggtatag ccccatctct acacctggct tatgtaaaca
      361 gagatgccaa taacccaagg atcccaaata ctcctcctcc atacctagag gtggcaaaga
      421 aaacactgaa gtcaggcgac gcaggcgagg aagtcttact cctacaaagt ttcctatctt
      481 ggcgcggtta ctggcccaaa ggagaacctc tttcgaaata cttcggtgag acaacaaaaa
      541 aagctgtgat gaattaccag atgaagaatg gactaccacc tgaaggtacg gtcggtccgc
      601 tcactcgaga agtttggaga aaggatatcg aaagggcttt gtaatacaca gttttggtgg
      661 cataagtctc cacttacata taaaatatag aggaaatgag ccttacacct gactcagctt
      721 ttgaagtttc tcgcccttgg ggtaaattcc tgcaatttac taaggacact ccttcgactg
      781 taaagatttt aaacataaat gccggagaag cctttagcct ccagtatcac aataagagag
      841 atgagatgtg gttcgttctt aaaggtgaag gtactatcga aattaatgga gagagcttcc
      901 ctgtcttacc gaatgcaaag tttcttatac ctaagggctc tacacaccga ataacagcag
      961 gagagagtaa tgtggaggta ttggaaattt ctaccggaga gtttgacgaa gaagatatag
     1021 tgcgtctaga tgaccgctac ggtcgggcaa caaatgagtg acatcattct caatcagatc
     1081 ctgattgtct gtatagcgac tttcctattg ctcttgctct ggctcctatc taagccggtg
     1141 ctcaaactcg tccactactc tcttaacaga gtaacgcaca atccagtact cgccccacat
     1201 gggctaaatg agtgggagac ggaagccgta ttcaacccag gggctttcgt cgatgacgaa
     1261 ggtaacattc atttactcta tagagctatg ggaggtgacg gactctcgaa gataggtcat
     1321 gcatggagta gcgacggcaa gaattttgag cgcaagtacc cataccccgt gtatcatcca
     1381 caaagaggtt tcggtttacc cgaggatgtc ttggtaccaa agaagttcga tccagtgaat
     1441 tatgcatcag gaggaggttg gggtggatgt gaggatcctc gcactgttgt catcgacggt
     1501 agagtgttca tgacttacac agccttcgaa ggatggaaca acgctcgcat cactctcacc
     1561 tctattgata tgcaagatgc gaaatctggt agatggaagt ggcaaagacc gatctatctc
     1621 tctccggcca atgagatgca taagaactgg gtcctcttcc ctgaaaagat taatggtaag
     1681 tacgccatct taaacagctt ggcaccaaag gtactcattg agtacgtaga tgatgtcatg
     1741 caagacaaga atatcaagag ctctcacaac catggcggct acggctacac agagctaact
     1801 agaaaaaata tgtgggacga aagaatgaag ggtgtaggtt ccccacctat caagacctca
     1861 ctaggctggt tggtgcttta ccatgcagta gaaccaaatg ttggatacag actgggagct
     1921 atgatcctag accataatga tccaaccaag atcctctacc gctctcctgc tccgatactt
     1981 actccagaca tgcattatga gaatgattgg aagccaggtg tcgtttacgc ctctggggca
     2041 gtagtagtga atggagaatt acaggtttac tacggtggtg gggacaaata catttgcatg
     2101 gcacacacca atctcgaaca acttctgctt tggttgaagg aatatggaaa gatttaaata
     2161 tataatgtaa gtaaatgttc accatcaaaa gatcagaaga caatccttat ctctcaccca
     2221 ggaaggaaca cccatgggag gcggcaggtg cttataattg gtgtccagta aagagtggca
     2281 agactactta tgtcgtatat cgcgcacttt cagagagaga acttcttgag gaacccaaaa
     2341 tccacaagtc agtaattggt agagcaacga gtactaatgg caaagacttc aaggaccgcg
     2401 tgcctttcat cgtgccagag catgagtggg aaaaatatgg ttgcgaggat ccaagaataa
     2461 caaaaattgg tggcaagtat tacatcttct acacagcgct ttctaattac ccctttgatg
     2521 caagtggcat taaggtagca cttgcaataa gcaaagacat gaagactatt gagtccaaac
     2581 atcttgtaac cccattcaat gccaaggcta tggcgctctt ccctgaaaag attaatggca
     2641 agtatgctgc ggtgcttacc ttccatactg acatgcctgg aacgaatgtg gcattagcct
     2701 tgttcgacaa gattgaagat atttggtccg aaagttattg gaaggattgg tacgagcaca
     2761 gagacagaca cgtgattgac cttcacagga gtaatgatga ccaagtagaa gtaggagcag
     2821 tacctgttaa gaccaaagaa ggttggttac tcatatactc acatgccaac cactacttct
     2881 ctcgcaatga gcactctttc ggagtggagg cagtaatact agacaagaat aatcccagac
     2941 taattattgg tagaaccaag ggagccttcc ttgtacctga actttattac gaagcgattg
     3001 gccttgtacc caacatcgtc ttcccctctg gagcactgat aaaaggtaag aacctagaaa
     3061 tttattatgg tggagccgac actcactcct gtaaggcgac agtaaagcta gatgatctgc
     3121 tcaaaagtat tttgcattca tcaaaacctt tagtgaatcg cttcgttggt aacccaatac
     3181 ttaccccaag actcgataag gaatgggagg cacatggcac cttcaaccct gctgccatag
     3241 accttggggg tatggtccac atactctacc gcgccatgtc gaccgatgat acctcgacat
     3301 tcggatatgc cgctagcaaa gacggtttag ttataagcga gagaagtgac atgcctgttt
     3361 atactccaag agcagacttc gaaaacaaac accaacaagg taactctggg tgcgaagacc
     3421 ccagactgat ggaaatcggc aaggaaatat ttatgaccta caccgcatac gatggttatg
     3481 tgccaagagt agccataacc aggattttga agacagaatt cttaaagaaa agttgggaca
     3541 agtggagcat gcccatcatt atcacaccca tggatgtaac caacaaagat gcttgcatca
     3601 tcccagagaa aactgctgct gggtacatct tcctacatag gatcaacgaa agcatttgtg
     3661 cggacatttt atcgtctctc gattttgaaa agcagaaggt ggtccgatgt attgagctta
     3721 tccaaccaag acgaggcatg tgggacggga agaaagttgg tatctcgggc cctccagtta
     3781 aaacacctaa aggctggcta ctcttctacc acggagtatc cgaaacctct acatacagaa
     3841 tgggcgcagt actcctcgat ctcaaagatc ccaccaccat actcgcacgt actgctatac
     3901 cactattcga gcccaaggag gactatgaga ccaaaggtat tgtacctaag gttgtcttcc
     3961 catgcggaca agtggttagg aaggacacca tataccttta ctacggagga gcagacactg
     4021 tggttggagt cgcaactctt tctctaaaag ctttgttgaa gatcttgtca tgagtgtaga
     4081 gcaatacaag aataaagaag tcttcatctt tgacctggat ggcactcttg cagtgagcaa
     4141 acaaccacta gatgatgaga tgcatgacct gctcgactcc ctactcgaca agcataaggt
     4201 gataattatc tctggtggac atcttaagca gttcgataaa caagtaataa agcatcttga
     4261 tttaactcca gaaaggattc caaacctttt catcatgcct gggtctggct ctagcatgta
     4321 tcgcttcaac ggcagtcggg aggagaagat ttatgcagac ctactctctc cagaggataa
     4381 agagaaaatt tattcggcat tctactacgc actcgaaaga gcaccattca acctacccga
     4441 gttccccttc ggagaaagaa tagaggacag agatgtagag atcaccttct ctgccttagg
     4501 ccaactcgct cccgtaaagg agaaggagaa gtgggaccca gatcatagga agcgagagat
     4561 tctgatcagg ttcctgctag agaagatccc tgaatacgaa atacacatgg gaggtatgac
     4621 atctatcgat gtcacgcaga aaggtataga caaagcctat ggcataagga aagccagcaa
     4681 ggaactaaac atccccatag agagaatggt gtttgttgga gatgcactgt atgaaggtgg
     4741 taacgacgaa cctgcaaaaa agactggtat tgagtgtata gcggttaaag atgtcgatga
     4801 gaccaagaag cttattcgtg agataataag tatatgaaga agtttctaat cctaattatc
     4861 atagttggtg ttgcttatgg cttgtattgg tacaaaacca ataatagcga ggtaaaaaag
     4921 gttgagatcg ctcctactgt gtctaagcct gacattgcta atgctaattt cctctttgag
     4981 ggtgaggaag tcaagctacg aaatggcaag tcttcaactg agatcgcccc tgatagtgct
     5041 gtgacaatag aaacagctct tagtggagat atcgtgtatg gcgatataaa taatgatagt
     5101 aagagtgatg cagtctctct cgcaacacag actggcggtg gctcagggct atttgtctac
     5161 ctcttggcat atgtttcggg aaatgtggag tacaagagtc tcgatgcagt cttcctcggt
     5221 gacagaatct ctccagagag cctcaaaatt gattctaacg gtaatgtact cgtgagctac
     5281 ttggatagga aggcctcaga gcccatgtca gccgaaccaa cccaattggt taaaaagact
     5341 tttaaaatcg tttcgggtcg acttgaggaa agatagtttt cgtgctttaa tatctcctca
     5401 tgcaaattag aaacatagcg attatcgctc acgtcgacca cggcaagact accctaactg
     5461 acgccctact acgccaaaat ggcatgttcg ccgaaggaga gagtatggac tctaatgctt
     5521 tggaaaagga gcgtggtatt accatctact ccaagaatac ctctctattc tataaagaca
     5581 caaagattaa cattgtggac acccctggac actcagactt cggttctgaa gtagaacgag
     5641 tactcaggtc tatcgacacg gtgctattgg tagtcgacgc ccaggaaggt cctatgcccc
     5701 agacccgttt cgtgctgaaa aagtctttgg aacttggcct taagccaatc gtagttataa
     5761 ataagataga caaaccagct gcaagaccag aagtagtaca tgagatggtg ctagagctct
     5821 tccttgaatt gggggcggac aacactcaga tcgacttccc tactgtatac gctattggac
     5881 gtgaaggtat ggccaagagg aatctcggcg acacatcgga caatttgaac cctctacttg
     5941 atgtagtact tgaacatgtc cctccagcag agcagaaact tgatgttcca gtgcgtctac
     6001 aagcatttaa cctcgcgtat gacaacttcc tcggccgttt ggctattgct cgtgtgtacg
     6061 aaggagtact aaagatgggt caagaagttt gggtacagaa gaacaatggc gaaaagtata
     6121 aaggcaagat taccaagctc ttcaccttct ctggtatcaa gagagttgag agcgaagagg
     6181 ctattgctgg tgacatcgtt atgcttgcag gactacctga gatctacatc ggcgagacta
     6241 tttcaggtga ccaaaatgca gaagtacttc cttttatcca tgttgatgaa cctacaatct
     6301 ccctaagatt cttggtcaat gactctccat tcgccggcaa agagggcaag ttcgtcacag
     6361 gtcgtcagat ccgcgaaaga ctcgaaagag agctcgaggt gaatgtgggt cttaaggtcg
     6421 acttctcctc tagtgagtat cttaccgtct acggtcgtgg agaactccac atcgccatac
     6481 tttgtgaaaa catgcgtaga gaaggatttg aactacaggt ctctcaacca cacgtaatta
     6541 tcaaagaaga aggcggtaag aaaaccgaac cattcgaaga ggtggtcatc gatgtacccg
     6601 aggaagcgag tggtaccgtt atctctactc ttacctctcg tcgtgcgcaa cttatggata
     6661 tgaagaccga caacggtcaa actcgattga tattcgaagc acctactcgt ggactattgg
     6721 gatacagagg ccagttcgtc gttgatacga aaggtcttgg tatattctcc tctcgattca
     6781 tcgagttccg cgcctatgca ggagtaatag accaccacga tgtcggctct atgatctcta
     6841 tggaaaatgg taaggcactc gccttctcac tcgacaactt acagcagcgt ggagtgctct
     6901 acattgagcc agctactgag atatatgagg gtcaggttat cggtaacacc tctaagggag
     6961 aggatatgta tgtgaaccca actaagggta agcaactcac caacatgcgc gcatctggtt
     7021 cagacgatgc aatcttcctc gtacctcctt acaaactaga tatcgaaaga gcgatggaaa
     7081 taatgaagga cgatgaatac atcgagatca ccccaacatc agtccgtctt cgtaagcaac
     7141 ttctcactca aaccgcccgc gccaaagcta ctcgaaagga tctgaagtaa taattgacat
     7201 taagccgcag atatagtatt aactaagtag atctacgtac caccacaccc caaagcgact
     7261 gtgtcgccaa ggagaaagtc agaatgtttg accttaccga aacggaaaag caagctcttc
     7321 gcacgaacct cctgaccagt ggtgtgtcgg gaatggtgat cggcctaatc ctcggcttta
     7381 tgtttggaat catggctggt atcgtggatg gcttgatggt cggactcaag atcggagtga
     7441 tggccggact catgatcagc tcactcatcg gtctgatctc agtgatgatt ggcggcccca
     7501 taagagccac caatagtctc acattcttcc tcggattcac ttctgtcttc gctttcttcg
     7561 tagctcttgg acactatgga gcaacgaagg cttttgggtc actgttcggc tttctggctc
     7621 ttcagatcgt cggaataatc ttcgtgttgc tcatcttcac gagtctcttt gcagacaaaa
     7681 gatctggcgc aaccacaaaa ggtttctagt tccacccacc cgcgtttgga tcacatccat
     7741 acacgggctt tttgttaatc tttaaatcaa actgcccgct ccaaagccac tcgaaaggat
     7801 ctgaagtaat ccttgacttt tgcatataaa tgtgctagtg tttaaataga actctcgcac
     7861 ctcactcaac cagtttggag ggcctaatgg ccagcagaaa cctaaaggat tggctataca
     7921 ccatcggcgt cctaacagcg gtgatgttca tgggctgggc catcatcaat atcccgatga
     7981 aggaccagta taaggacacc gtagtcagca ctgctgaggc aagcacgatc tcgccccgcg
     8041 cggaacccgt gatctacttc tcagacatgg ctggctcgct cgagcagccg tnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcgcggaa cccgtgatct acttctcaga
     8221 catggctggc tcgctcgagc agccgtcgcc gaacacgatc agtgtggcga cacctagcgt
     8281 gcacttcgtg gtgacacgca ccgagaagag cagtcagctc gaagaaggca tcctactcaa
     8341 cctcgtgaga actaccccgg gtgtggatgt ttacggtagc tcgatggacg cggacgaagc
     8401 cagtcttgtc gttgtcggca agcctctcaa cctccgtcgt aatgtcatca tcatgagtca
     8461 ttatcgtttt ctgatcatgg tgaaggatca aggcagtcgg gtgctgcaat gcaatgttga
     8521 ggtcccgatg catcgcctgt cgaatggtat gatcgtgatc tgcagttgaa ccaccgagct
     8581 ccggctcgca cccgcttggg agattaattt ctccctggcg ggctttcgtt ttatattttg
     8641 tactctccta ctctggtgcg tttgatactg tatgccaagg cacttgtgcc gagcttctga
     8701 cccatctcat gtgctagaga acggatgtag gtgccactag agacactggc tttgaagtga
     8761 gatattagaa ctttttcccc ggggattatt acctctctcc acttatcttt tatctctgtt
     8821 tgtctgaagt cgcccttgac cagatcaatc ttgtccaaga tttctttgag tatatcttcc
     8881 tgtttttcta tttttgtatt tatatgctct atcgagaaaa tctttatccc cctctccggc
     8941 gtctctagat cacttgtctt acctcttact ttcataaagt ctttgttgaa taccttggaa
     9001 gaaaagattg ggtacttctg cgtttttttc tccctcatct ctattagcaa cttctccatc
     9061 ttgaaatcga gtttagctgg catggcgcca gtattggtaa cgagaccgag gatgtcatag
     9121 gtatctgtct caaaccccca aagcacttcg aactcatagg tcttgtcgag actcaagtat
     9181 ttatctttat ccttagtatt acccgatagg acgagcataa caccctcggc catagggtcg
     9241 agtcgtccaa gatatgtcat gttagtttcc tcatatcctt tgttcttcgc tctaaatctc
     9301 tcgatacatt cgagaggagt ctcaccaaga ttcttatata gttttataac ttctgaactc
     9361 ataattatga aaatggtccc gaaactactt cgggaccctg actgctgggg gcacatgcac
     9421 cactctcacg atcggccagt agtaccacga gaaagcgacc atgagagcga tggcgaccgt
     9481 gggcataagc caccatgtag caccacgatc cttcgtccaa tcgtactcat cccagtcttc
     9541 cttttcgacc aagttttttt cgatcatgat tcctccaaag ggttcacttc tggcaacaaa
     9601 ataacccaga atctgcataa agtcaaataa gatttgaaaa agcttaattt ttgatataat
     9661 tctctcatgt caaaacttga tactgcccca tataaggggg ttcgcgactt ctaccctgag
     9721 gatatgagga tacagaattt catcttcaac acctggaaga aagttgtaga gcgaaagggt
     9781 tatgaggagt acaatgcctc acctcttgag cacaccgaga tctatgctga gaaatcgggg
     9841 gaagaaatag tgcgtgagca gaccttcacc ttcaaagacc gaggagacag gtctgtcacc
     9901 ctacgaccag agatgactcc aacactcgca agaatggtcg cagctagaag aagaggtctc
     9961 aaattccccc tcaggtggtt ctctataccc aacttgttca gatacgagaa gccacaaaga
    10021 ggtcgcaagc gcgagcatta tcaactcaat gcagatctct taggagtacc tggtctagat
    10081 ggtgacaaag aaataatcac gctactttac gagatcatga aagagtttgg tgccaaggac
    10141 tctgacttcg aaataagagt gaacaactgt aactttgaag acttcaagaa ccttaccccc
    10201 aacatggcat ttgatgaaac tctagctcgc gggcaggcat actacacggg tacagtcttt
    10261 gagatatttg ataaggaccc agagaaccct cgcgctctcg ctggtggtgg tagatacgac
    10321 aatctactcg aactctttga tgtagagcct gtgcctgcag ttggcttcgg tatgggagat
    10381 ataaccatga gagacttcct agaaactcac aagcttcttc ctaatgcgta aaattgtttt
    10441 cgatattgag actagaaacg ttttttctga tgttggtaag aacgacccaa ccctattaga
    10501 catttctctc gttggagttt gggattctga aactgatgct tacactagtt acctgcaaga
    10561 agacttgagt ctactttggc cgatactaga gcgggccgac atgcttatag gattcaactc
    10621 tgaccacttt gacatcccac tactcaacaa atactatccg ggcgacctca ctaaacttaa
    10681 gagcctagac atcttgaagg aaatctacaa aacctatggc agaaggatga agctcgacca
    10741 aatcgccgaa ggtactctag gcaagaggaa gtcaggcaac ggtcttgagg ccatagaatg
    10801 gtggaagaag ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtctcgac
    10921 gatgtccgga ttaccaaaga ggtttacgac tatgccttgg agaacaaaaa aattattttc
    10981 aaagagggtg gtaaactcta cgatgttaaa ctcgacacta gtaactggga ggctccgagc
    11041 gatcacagaa taacttacac cctaccattc taatgaatta cttttttatc tttggagcaa
    11101 agtatttatt cttcctttca gtcattattg cagctattta cttcttcaaa tctttggaca
    11161 agagaaagct atttaagttt ggagtcatta ttctgcctgt gtcttatata tttggtctcg
    11221 ttgcaagaac tctatactac aatccaagac cttttgttac ggagaacttt accccactga
    11281 ttgaccatgt agctgacaac ggcttcccct cagaccacac cctgtttgta gcgtctttgg
    11341 cagtatggac gacattgttc aataagaaac ttggtcttgt tttgtggtgt attgccctcg
    11401 tagttgcgat ttcccgagta tatgttggag tacaccaccc agtagatgtg ttggggagta
    11461 tagtgatttc tgcattagta gggctaatgt tatactgcat aaataaatat ggagaacaac
    11521 ccaacaagta acaataacaa tgtctctgta cccttagcta taatccttgc tggaggtatg
    11581 atcgcggcgg ctgtgtattt gggtggcagg actccactac cagacacgaa caacaaccta
    11641 gccaagactg aactccccga catcgaacct attagttctg gagacaaggt atttggtaac
    11701 gcatcagcaa gcgtggtagt agttgagtac tctgacctcg aatgtccgtt ctgcaaagtc
    11761 ttccacagca ccatgcatca attggtagat gaatacaaag gcaaggtggc ttgggtttat
    11821 cgtcactacc ctatcaagga gctccacagc agagccacta aagaggccga ggcgtcagaa
    11881 tgtgcttatg agcaaggtgg gtcaacaatc ttttggaaat acatcgacaa agtctttgct
    11941 accacaggta caaacgacac tctagaccca cttgaactac ccaagattgc agggagtttg
    12001 ggactaaaca ccgatgtatt caacacctgt ctaaacactg gtaagtatgc agccaaggta
    12061 cagagtgaca tcaagaaagc tgtggaagca ggggcacgag gcacacccta ctcagttata
    12121 ctcaaggacg gcgtagaagt agagaccata aacggcgcag aacccataga ggatgtgaga
    12181 gagaagttag acacagctct cggtaattaa tgtttaagta aggctcgtat tgagcaatac
    12241 tcacaatcct ccttcccaca agtgctcttt agtatctctc cactccacac ggatagagca
    12301 agactatcta tctctgtatt taccttatct atatcggcat tcgccacatc aagagtaata
    12361 ataggacatc tctccttttt atctggcctg atgaagacaa gactctgagt cgacctcttc
    12421 aacttccact taggattagc caggacaagc atcttgtaga aaacaagttg cctaaagtag
    12481 tctccgttcg agttcttagt ctcacccttg atctcattta ccgacatggc ttgcttactc
    12541 ttgtagtcat acacctgcac ctcatcgccg gtgtcaacaa tagtgtctaa tttgccgtgt
    12601 attggtatgt agagtcctgt ctcaattttg tttgcattgt gtactgcttc tagccacttc
    12661 tctgtataca ctgtacccct ggttttcaaa tgtgtttcta actcacgcgc aattgctgga
    12721 gcatcttctc taatatcatt cattacaagc tccttgtccc tcatggagag gaaggtatcc
    12781 tcgatggacc tctcggcaac actcaaaaca attttctcta tctcattctt atcaagactc
    12841 tctctgccct cgtttatcca gatgtgcgag agcgcctcat gcatggctgt accacgagct
    12901 gagttaatac tcggtgcctg aggcatctta agaatactct cgtatataaa tttgttagga
    12961 cactcgagaa aatggtttag cgctgtcaca gaaagaccgt tcttcatcag tagctctttg
    13021 gccaaagcaa gtactttgga gtcatagcta gcttccttgc tcttttttct gtctaccact
    13081 atctcaagtt tcttcctcgg catacttaca gccgtagcac ctgactcaat ctccgatata
    13141 aacctaagtg gtgtaagcac cttgccatct gactcctcaa gtggagtaag cactaccaca
    13201 tgctttcttg ctctggttag cgctacataa aacaacctcc tcatatctcg tatatcacca
    13261 ccgacaacat ccttagtcgg taagacgaag gaactcccgc gattcctacc tacccaggct
    13321 tcgtctgtag catatggtat gaaaacataa tcgaactcta aacccttaga accatgtgcg
    13381 gttaaggccc tcaccttcaa atccggagca ccgacactca ctttgactgg cttcatttct
    13441 gctgactctc ggtaagcaag catgagtctt aagagctcgt ttggacttgc cacctcggaa
    13501 tctctagcaa gtgactcagc caacgagaca atacccttcc acacatatac ataagacgga
    13561 tcaagtgtca ccaaaccagc gaatccgctt atatcaccaa gtttgataat aaactcaata
    13621 ggtgaagagg aaactgcttc tgatctaatc tccttcaatt tatgttcaat tacctttagt
    13681 tcaccaagct tccccgacct taatgcttta gaaagcgcaa tactctcgtc gaaactgagt
    13741 ccccacatac ccgtagacaa agtgcgtgcc agatactcgt atttatcatt ttcatatacg
    13801 taagttataa ggtcgaaaaa ggttacccca ataggatggt ggaatatatc aacactcctt
    13861 tcagatgaca ctgggataga gttgaactca agtagtttta ttaccctttc aagatccctg
    13921 tttcttttag taataattgc gacggttgcc tcaagttcat ctttagtaat tctcttaagc
    13981 tcatcgacta gataactctc catagtccga gtatcctcga cactaacaat ctcaattggt
    14041 ctttctgact cagcactctc ggacacaagc cttacccgaa ggtcgtcatg ctcaccctct
    14101 gtataattcc tctcaatgat agagaagctc gaatctagaa tgctttgatg cgagcggtag
    14161 ttagactcta gagaaatcaa tttcatctct ggccaacgct tcctgagaag gaggaaattc
    14221 tcaaccgatg caccctgaaa gcggtatata gcctgcttct catctccgac aataaagacg
    14281 tttggtgttt cgaaaaagtc tgcaagcatc gagacaatgt agttttgtgc atcgttcgta
    14341 tcttgatgct catcgaccaa aatatacaaa aatctttctt gaagtaacct aagaaataga
    14401 ttatcagtct taagtgtgac aagaagttgg attataaggt cgtcgaaatc gagcttcctc
    14461 aacttcttct taccctcctc atactcctca taaatatcag cgaagattaa agttttcttg
    14521 cacttctcta tcctctcttc tgcatcggcc ttgagcttgc ctttggtggc acctcgagtt
    14581 gaaatagagt cttcgtcatt tgtaattctt tctatctccg aattagcaaa cttcctgaca
    14641 tcttctgggg ttatggcatc acgctttgag ttactaatac tacttatgat ggctgcgagg
    14701 tatgcatcgg gcttaccact tggtcgtaga tctttgtgtt ttggactctc aataatttca
    14761 cgaatgagca tctctttgtc tacatctgtc atctgcttca tatcactaag ttcaatgaag
    14821 tgatcttgat actcgttcat catcgccttg gcaaagctgt ggaaggtatg catctcaacc
    14881 tcgtgagcgc gagtgcctat aaggttacga agcttgcccc gcatagcctt gacccctgcg
    14941 tctgtgtaag taatggcaag gattccgctt ggaggcgtgt cggtctgttt gagtatgttc
    15001 gctatacgga gagtaagaac cgtagtctta ccggtcccgg ggccagctac gaccattaca
    15061 gggccctcta tagtgtctac agcctccttt tgggccttat tcagggattt ataagctttg
    15121 gcaaagtttt cttccatttg agcctatttt agcatggttt atttcctcct tgacttttac
    15181 accatttgat gctattatta aaacagaccc caacggagaa ccgtctgtgc ccaagatgat
    15241 gaaatacacg atcagcgctg gacttgcggt attcggactt cgagtgatgt gggaatggtg
    15301 gcaggtctgg acacacccaa tcccccacat gccgcttgca gagtcattgc tcctgctagc
    15361 gggaggcatg ctgctcatcg ctgcccacgc cacgttcaag atgaaatctg atcggaatta
    15421 aacaaagggc gactcttcgg agtcgccctt aatcttttaa taacccctga gtctatttat
    15481 cttttcgtgt tggcgaatct tctcgtgtgc cttagcttca atttgtctga tacgctcgcg
    15541 tgtgacacca aagaccttac ccacttcttc tagagtgtgg gtgtaaccgt ctgtaagtcc
    15601 gtgtcgcatt tctagtatct ttctttcttt tggaggcaag tcattcaaaa tttctttaac
    15661 ctgatcagaa ataattcttc gtgaggactc ctctgctgga gagagtatct tatcatccgc
    15721 caggaagtca cctagtttac tcttgccatc atctccttca tcaccaaccg gactctcgag
    15781 cgacactgta gattggtcga tcttctctat ctggtaaatc ttatcgacat ccagattcat
    15841 ctccattgcg atctcctctg gcattggctc gcgaccaagg tcttgagtga gacgtcttac
    15901 aacttgttta tacttggcaa tggtctcaac catgtgcaca ggtacgcgga tagtacgaga
    15961 ctggtctgca agggcacgag taatagcttg gcgaatccac caggtagcat aggtagagaa
    16021 cttgaatccc ttagtacggt cgaacttctc cactgccttg tataggccga ggttaccctc
    16081 ttgaatgagg t
//
LOCUS       DAORIT010000016        16001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_617_length_16001_cov_46.042895, whole genome shotgun sequence.
ACCESSION   DAORIT010000016 DAORIT010000000
VERSION     DAORIT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16001
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_617_length_16001_cov_46.042895"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /gene="rpsI"
                     /locus_tag="PLD99_02025"
     CDS             complement(<1..155)
                     /gene="rpsI"
                     /locus_tag="PLD99_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRH25712.1"
                     /translation="MADKAERYIGATGRRKTSIARVRITPAAKTHIEVNERPFEDYFP
                     VAELRRNV"
     gene            complement(161..505)
                     /gene="rplM"
                     /locus_tag="PLD99_02030"
     CDS             complement(161..505)
                     /gene="rplM"
                     /locus_tag="PLD99_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRH25713.1"
                     /translation="MKYTFDATDKKPGRLAAELAVLLMGKNKADFAKNVVTDVEIEVT
                     NANSMSFDAKKLIDKKYASHSGYPGSLKLPSMKQMIEKKGGKEVLRRAVLGMLPKNKL
                     RPRMIKNLKITD"
     gene            complement(510..857)
                     /gene="rplQ"
                     /locus_tag="PLD99_02035"
     CDS             complement(510..857)
                     /gene="rplQ"
                     /locus_tag="PLD99_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRH25714.1"
                     /translation="MRHHNANRKFGRVAGQRKALMKSLAHNLVLEGKIKTTEAKAKEL
                     RPYVEKLVTMGKKETPASKRLLTSRVGKMAAEKIAGSLATTYKKREGGYTRITKLVRR
                     DSDGAKMAVIEFV"
     gene            complement(877..1833)
                     /locus_tag="PLD99_02040"
     CDS             complement(877..1833)
                     /locus_tag="PLD99_02040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRH25715.1"
                     /translation="MADHTILLPSKPKIVSEEGLSGVYEIEGLYPGYGHTLGNSLRRI
                     ILSSLPGVAITSVKITGVSHEFSVIDGIKEDVITILLNLKKVRMRFSTDEAQTLTLKI
                     KGDKEVTAADIVLPGQVEILNPDQHIATITDKSTTLEIEMRAEKGLGFIQKDVHQKER
                     VDIGFIALDAIFTPIRRVSYEVENMRVGDRTDFNKLKLSIETDGTISSNEALTKSIEI
                     MINQLKAIIGFKEEEVPMSSMPTAEHDGMKSDLDPEILKTRVESLDLSSRTQNALNNG
                     NIRTLGGLARKKEKDLLEIEGLGSKGIQEIKKALAEFGITLK"
     gene            complement(1858..2463)
                     /gene="rpsD"
                     /locus_tag="PLD99_02045"
     CDS             complement(1858..2463)
                     /gene="rpsD"
                     /locus_tag="PLD99_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRH25716.1"
                     /translation="MKIGPKYKLARRLGAPIFEKTQGPKYALSLSKKEKNDQMPTRPK
                     SEFGKQLIEKQKARFSYGVTEKQFAKYVKKSLSAAEPTQKLFQALESRLDNVLYRAGF
                     AKTRLQARQMASHGHTTVNGGRVTVPSIELREGDVVSVRDGSQKSALFTGVDERLKQA
                     PVPAWLTVNPDKVEAKVMGKPVYEKSASVFDLGAVIEFYSR"
     gene            complement(2473..2907)
                     /gene="rpsK"
                     /locus_tag="PLD99_02050"
     CDS             complement(2473..2907)
                     /gene="rpsK"
                     /locus_tag="PLD99_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRH25717.1"
                     /translation="MGKKRIIETEGKEETKPAVAAQPKTSGKKRLETGILNVESTYNN
                     TKVVLADEKGNVVAWSTSGALGFKGAKKGTPFAAAKIGEVIGDKALLLGVKEVSVNVR
                     GVGSGRESAIRGFISKGINLTTITDRTPVPHNGPKPPKPRRA"
     gene            complement(2914..3294)
                     /gene="rpsM"
                     /locus_tag="PLD99_02055"
     CDS             complement(2914..3294)
                     /gene="rpsM"
                     /locus_tag="PLD99_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRH25718.1"
                     /translation="MRLLGITIPEDKRIDIGLTILFGIGRSRAAAILSEAKVDSSKRP
                     KDINIEEENKIRSIIEKITLEGDLKREVQANIKRLKDIKAYRGVRHQRGMPVRGQRTK
                     TNSRTRRGNVRKTMGTGRRAVEKT"
     gene            complement(3295..3411)
                     /gene="rpmJ"
                     /locus_tag="PLD99_02060"
     CDS             complement(3295..3411)
                     /gene="rpmJ"
                     /locus_tag="PLD99_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRH25719.1"
                     /translation="MKVKASVKKMCGQCQIVRRKGYVYVICKANARHKQRQG"
     gene            complement(3460..3675)
                     /gene="infA"
                     /locus_tag="PLD99_02065"
     CDS             complement(3460..3675)
                     /gene="infA"
                     /locus_tag="PLD99_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRH25720.1"
                     /translation="MPHNPELERTGTVTEALPNIMFKVKIDGSDEEILTYLSGKMRLH
                     RIRVLVGDRVILELEPYGGKPRIIKRL"
     gene            complement(3676..5004)
                     /locus_tag="PLD99_02070"
     CDS             complement(3676..5004)
                     /locus_tag="PLD99_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25721.1"
                     /translation="MPKKIEDVVHSNRKSIRDIPIPIRKTDDVRPVAREINRETARET
                     FINPQKSVGTAMSTPQVIRSEPRLSYVEPVKNNFSGAKRIIGGILVVVLIVLMFITLK
                     NGAVLTYKPRVTQVAFANDTYSAYPNASTSALVYSTIKLSSTKSLDVEAAGDETVSEK
                     SKGRIAIYNEQTTDQQLVKSTRFESTDGKVFRLQEDVTVKAKSNLEVEVIADQPGKEY
                     NITLSDFTLPGLKGTARFAKVYGRSKTNMTGGFIGLRKKVSQDTLNTAKTALESEAKA
                     DLDAQIQAQTPEGYILFPGLNKVTYELLPTEAGDQGKAKVTLRGDLSAWIFKKTDLAQ
                     YLAVQKLGADKVGAPVDFLDTSKLSASLVNTEATSTQAIKLSFIGSATLISVTDEASL
                     MSDIKGKEESELFEILKGYPSIVEASAVIRPFWKSSFPTDETKIKIEVQK"
     gene            complement(5021..6178)
                     /locus_tag="PLD99_02075"
     CDS             complement(5021..6178)
                     /locus_tag="PLD99_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25722.1"
                     /translation="MSLWFSGKKDFNILVIDISSGSTSGALVATSKGKVPRLLTAIES
                     EYNFKVVDSAFSPDKAMLNSLGIVLDFIVKKISTMKKVRVDKAVITFSSPWVDSYLKT
                     VEIKKEKEFVFDEKFMLGVINEEKSIFQNNLRESFSEESEIFEAGVMDLYLNGYPASE
                     PKKESVDEVEISFILSASTKDLLTRIEDQIIKTLALRKGLVMHSFMYVFYKVLSHSFN
                     NLHSALLINMTTHITDVLFLRHNNLALNANLPFGPASIIEAVAGKLNIPSEVASSYLT
                     LFAEGALDKDTTESIDKILTENETVWRDLWAEVKEKIIHTAEVPYSIFLVTDPKFGKL
                     MKTFLESVFPDKNIILIGDTNTFTKELVENSAKESKDERILLLSSFANLIS"
     gene            complement(6202..7917)
                     /locus_tag="PLD99_02080"
     CDS             complement(6202..7917)
                     /locus_tag="PLD99_02080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRH25723.1"
                     /translation="MESTEIRRRFLSFFEKRGHAVIPSASLIPENDASVLFTTAGMHP
                     LVPYLLGEEHPKGKRLVDVQKCVRTGDLDDVGDNRHFTFFEMLGNWSLGDYFKEDAIK
                     WSYEFLTSKVEGLGLDPTKLYVTVFEGNDDAPFDEESKKIWMSLGVPEHRIYALGAED
                     NWWSAGENGPCGPDTEMFYDLTEGLGDLNKDEFLKAVKREDLVEIWNDVFMEFRKENG
                     KVVEKLKSKNVDTGAGLERMTAVMQGKKTAYDTDIFEPIFNALGKTSDNVSARIIADH
                     IRASVFMISDGVVPSNTDQGYILRRLLRRSFIHAQKLGLDSINLIKVAEAIIDHPVYS
                     SYTLNRDLTNNVIDAEIGKFKETLKDGLKQFEKGADPFDLFTTYGFPFELTKELANDR
                     GVEINEEDFKQKFQKHQELSRAGAEQKFKGGLAGHSEIEVRYHTATHLLHQALRDVLG
                     NEVSQKGSNITPERLRFDFSFSRKMTDEEKKQVEKIVNTKIEEKLDVTNIVTSKDEAV
                     KMGALHFFDQKYPDQVSVYKIGDYSKEFCGGPHVKNTGEIGKFKIDKEEAVSAGVRRI
                     KATLL"
     gene            complement(7933..>8148)
                     /locus_tag="PLD99_02085"
     CDS             complement(7933..>8148)
                     /locus_tag="PLD99_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRH25724.1"
                     /translation="IATLGRFSMEYVMKRYGLSEEIKAISDIHGQVLKSPNGFKLVPL
                     FHPAAAIYNQKLRPVLLDDFKVLKTLI"
     assembly_gap    8149..8248
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8249..8642)
                     /locus_tag="PLD99_02090"
     CDS             complement(<8249..8642)
                     /locus_tag="PLD99_02090"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRH25725.1"
                     /translation="MEEKVISMDGELADLRRAGDYLPVIGEGSTTPKIMFIGEAPGDK
                     EAKTGKPFVGRAGQVLNELLESVGIDRESVYITNIVKDRPPKNRDPKPEEIELYGPFL
                     DEQIETMKPAVIATLGRFSMEYVMKRYGL"
     gene            complement(8645..9334)
                     /locus_tag="PLD99_02095"
     CDS             complement(8645..9334)
                     /locus_tag="PLD99_02095"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRH25726.1"
                     /translation="MTKLFFDIETIPASEESMEALKYLYDKKISKVEPDSDKIQDTFE
                     KFVEKTSFDGAYGRICCIAYAINDKPAEYLSGDEKQMVKKFWEIAGKVDLIIGHNIRD
                     FDLPFIMQRSAILGVKPTWNKFEEPGKKPWDMVKFLSFARYKNAPIFDTMHEWDNWSG
                     YKKSLEHLCYAMGVPTPKEGIDGSQVWNFYKAGKIKEICEYCARDVEATRAVYKKMTF
                     ENAEETQFEPF"
     gene            complement(9336..9770)
                     /locus_tag="PLD99_02100"
     CDS             complement(9336..9770)
                     /locus_tag="PLD99_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRH25727.1"
                     /translation="MTEALAPQYWHLLIALTLGVIIGLERTIAHKTAGMRTYGLVSMG
                     SCLFVILSQLVLADSGMVNLDPMRTAAGVIMGIGFLCGGVIIFQNHELTGLTTAAGLW
                     VSAGIGMAVGFGQVGLAIFTTFITLIVFTLLWTVEHLITRKN"
     gene            complement(9767..10465)
                     /locus_tag="PLD99_02105"
     CDS             complement(9767..10465)
                     /locus_tag="PLD99_02105"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRH25728.1"
                     /translation="MTDIIKADGTREIFNPSKLRTSLIRARASEEAADAVIAQVMSEL
                     REGMTTREIYKSAFKHLVEMQKPVAQRYSVRRAMLDMGPTGFPFEDFVAEVLRTKGFE
                     TLTRQTVLGGCVPHEMDVVAWNDDKLVMVEAKFHNGLGIKSDLKVALYVKARFDDLKD
                     NMHHYGGRDRKLDEGWLITNTKFSSTALHYGLCQKLTMIGWNYPERGNLQDMIEENPE
                     LLDLIFKKHIEQLQ"
     gene            complement(10466..11560)
                     /gene="mnmA"
                     /locus_tag="PLD99_02110"
     CDS             complement(10466..11560)
                     /gene="mnmA"
                     /locus_tag="PLD99_02110"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRH25729.1"
                     /translation="MTKKARHIMLGHLMNTSKISRNTSKKVFVGLSGGVDSAVSAALL
                     KEEGYDVTGVFIKVWQPDFLPCTWRDERRDAMRVAAKLDIPFLFFDFEKEYKNGVADY
                     MIEEYRKGKTPNPDVMCNREVKFGAFWDRAKEMGADYIATGHYARNIDNELYEGVDKE
                     KDQSYFLWTLTKDDLEHVLFPIGHLKKHEVRKIAKKHGLHTAEKKDSQGICFIGDVDI
                     EDFLSHYIETKSGEVLDTKGNIIGSHTGALYYTLGERHGFEITEKVSDSKPYYVISKN
                     IEKNTITVSNDNEEILKSKSEEVNLERLNLVAMKLPVDIEARIRYRQPKFKVSLENNI
                     VQFLEPQTVDSGQSIVFYSGERCLGGAIIA"
     gene            complement(11487..12365)
                     /locus_tag="PLD99_02115"
     CDS             complement(11487..12365)
                     /locus_tag="PLD99_02115"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRH25730.1"
                     /translation="MKDIFRDKEIYLVVGLFLFVLFFAHIISPPNAFKEESILNIERG
                     LSLNQVSDVLKNSNVIRSRVLFNTFVTLFGTQSGVKAGEYYLHYRESVIRLAWRMVRG
                     EHNIDEVKITIPEGFDNTEIASLFDERFPLFNKKMFLDLGEQGHLFPDTYFMKVSVTA
                     EKVVEILNSNFESKTLLFEDKFDASKYSKEEIITMASLIEGEVQTKEDKELVSGILWK
                     RLSIGMALQVDSAPITYDEPGLPKKPINNPGLVSIEAALSPKSSPYLYFISDKEGKTH
                     YARTLDEHIQNIKKYL"
     gene            complement(12365..13606)
                     /locus_tag="PLD99_02120"
     CDS             complement(12365..13606)
                     /locus_tag="PLD99_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25731.1"
                     /translation="MLDAFFALLTSKEFYLQLAHILYVTSPVWIPIIFISVAFQIWMN
                     LKRREWVAAQGSVLLEIKLPRELMKSPLAMELFLNHLHQTSAGTLIDVYLKGRVRPWF
                     SLELVSIGGAVKFFIWMHKSFRARVEAQLYAQFPNIEIHEAEDYSLGVKRDPEKMSIG
                     YFGQFTLTKPDAYPIKTYIDYGLDKDPKEEFKVDPMVSVIEYLGSLKRGEQAWIQILI
                     RGHAKEGLKLGRIVVKPDWKKDIEKEIAKILKEGRFKSAEEKNISGQHMSEGQKDLVK
                     AIERTTGKLAFDTAIRATYLAEKDAFNPANIGGIIGSITQFGSATLNGFRPSFGTGYD
                     YPWQDPFGKKKANNERELLEAYKRRSYFELPFKHIGGKPFILTTEEIATIFHFPGEVA
                     GTPTLTRIPSKKAEAPANLPI"
     gene            complement(13626..14663)
                     /locus_tag="PLD99_02125"
     CDS             complement(13626..14663)
                     /locus_tag="PLD99_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRH25732.1"
                     /translation="MSEKITLSFKPKAVTKRLLSVLNKRGADVVENRYGLGAKSVRMT
                     LDAIGKKYGITRERVRQIENHSLSLIRKSKEYKSEQVVFDELKAVVVELGGLVSEKDL
                     LKHLAKDESGQNHIHFLLVVGEEFMREKEDEEFKHRWHVDSDLSKRIHASLRKLYSKL
                     SDDELVLEGDLINDFLEEVKDMNDKYKNEEVIKRWLSISNKIGKNPLGEWGRTDSPNV
                     NAKGMRDYAFLVIRKHGSPIHFKEVAKAISQYFNKKAHVATTHNELIKDPRFVLVGRG
                     LYALAEWGYMSGVVKDVIKKIIDKNGPLTKEEIIKKVLKERYVKENTIMVNLQNPKYF
                     KKDKEGRYNIA"
     gene            complement(14702..>16001)
                     /locus_tag="PLD99_02130"
     CDS             complement(14702..>16001)
                     /locus_tag="PLD99_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRH25733.1"
                     /translation="SDYGTGAIMGVPAHDERDKVFAKKHDLPIVTDYKKAGFEDFGNK
                     VIKYKLRDWVFSRQRYWGEPIPVIHCAKCGAVPVPESDLPVLLPEVANYQPTDTGESP
                     LATIDSWVNTECPKCGGDAKRETDTMPNWAGSSWYFLRYMDPKNGESFASKDALKYWS
                     PVDWYNGGMEHTTLHLLYSRFWNKFLFDIGLVPTSEPYKKRTSHGIILASDGEKMSKS
                     RGNVVNPDMVIEQFGADTLRVYEMFMGPFEQAIAWSEASLVGPRRFIERVWKLGNKVG
                     GVETDNYSMHSAIKKVTLDIEGMGFNTAVSALMVWVNELDKKTSISKEEYETLVSLFA
                     PFAPHVAEELWSILGHKESVHTASWPTYDESKLVKSEIVYAIQINGKVRSEISVSKDQ
                     DQIEIEKLALSNEAVSKWLEGKEVKKVIVVPGKVVNIVVA"
BASE COUNT         4152 a         3803 c         3016 g         4930 t
ORIGIN      
        1 acattccttc taagctctgc tactgggaag tagtcctcga aaggtctttc attcacctca
       61 atatgagttt tggctgcagg tgtaatgcga acacgagcaa tagaggtctt cctgcgtcct
      121 gttgcaccta tgtatctctc tgctttgtct gccatataag ttaatctgtg atttttaaat
      181 tcttgatcat gcgaggtcgg agcttgttct taggaagcat accaaggact gcgcggcgaa
      241 gcacttcctt accacccttc ttctcaatca tctgcttcat agaaggaagt ttgagagaac
      301 ctgggtaacc tgagtgcgag gcgtacttct tgtctataag cttcttggcg tcgaaggaca
      361 ttgagttagc attggtcacc tcgatttcaa catctgtaac aacattctta gcaaagtcgg
      421 ccttgttctt acccatgaga agtaccgcta gctctgctgc tagtcgtcct ggtttcttgt
      481 ctgttgcgtc gaatgtgtat ttcatatatt taaacgaatt ctattacagc catcttggca
      541 ccgtctgagt ctctacgaac aagcttggta atgcgagtgt agccaccctc acgtttcttg
      601 taagtagtag cgagtgagcc tgcaatcttt tctgcagcca tcttgcctac acgagaggtc
      661 aaaagtctct ttgatgctgg agtctccttt ttgcccatgg tcactagctt ctcaacataa
      721 ggtcgaagct ccttggcctt ggcctcggta gtcttgatct tgccctcaag aactaggttg
      781 tgcgccaaag acttcatcag agcttttctc tggcctgcta ctcttccgaa ttttctgttg
      841 gcgttgtgat gtctcatagg agcactaatt tatacactat ttgagcgtaa ttccaaactc
      901 ggccaaggct ttcttgatct cctggatacc ctttgagcct agaccctcaa tctcaagaag
      961 atccttctcc ttcttccttg ctaggccacc tagggtccga atattgccgt tgtttagagc
     1021 gttttgagtt ctacttgata ggtctaggga ctcaactctg gtctttagga tctcagggtc
     1081 taggtctgac ttcataccgt cgtgctctgc tgtaggcata gatgacatgg gtacttcctc
     1141 ctccttgaag ccgatgattg ccttaagctg gttgatcatg atctcgatag acttagtgag
     1201 ggcttcgtta gaagagatgg tgccgtcagt ctcgatagag agcttgagct tattgaagtc
     1261 tgttcggtca ccaacgcgca tgttttccac ctcgtagctt actcttctta taggggtaaa
     1321 gattgcatcg agtgcaatga agccgatgtc tactctctcc ttctgatgta catccttctg
     1381 gataaatcca agacccttct ctgctctcat ctctatctca agagttgtag acttatcagt
     1441 gatagtagca atatgctgat ctgggttaag gatctcaact tgaccaggaa gaacaatatc
     1501 agctgctgta acctccttgt cgcccttgat cttaagagta agagtctgag cctcgtctgt
     1561 agagaaacgc atgcgaacct tctttaggtt aagaaggata gtaatcacat cttccttgat
     1621 gccatcgata acagagaact catgtgagac tccggtaatc ttgactgagg taatagcgac
     1681 gccaggaaga gacgagagga taatccttct caagctgtta cctagagtgt gaccgtatcc
     1741 agggtaaagt ccctcgatct cataaacgcc agagagtccc tcctcagaga cgatctttgg
     1801 cttcgaaggt agaagaattg tgtgatctgc catatgtttg ttaaataaat attaaaatta
     1861 tctactataa aattcgataa ctgccccaag gtcaaatact gatgcactct tctcatatac
     1921 tggcttgccc ataacctttg cctcaacttt atctggattt acagtgagcc aagctggcac
     1981 tggggcctgc ttgaggcgct catcgacgcc agtgaagaga gcactttttt gactcccatc
     2041 acgcacacta accacatctc cctcacgaag ctcgatcgaa ggcacagtca cacgaccacc
     2101 atttaccgta gtgtggccat gagaagccat ttgtcttgct tggagacgag tctttgcaaa
     2161 gccagctctg tatagaacgt tgtcgagtct agattccagc gcttggaaga gtttttgagt
     2221 tggttcggca gcacttaatg atttcttcac atattttgca aattgctttt cagtcacacc
     2281 gtaggagaaa cgagccttct gcttctctat aagttgctta ccgaactcag acttaggtct
     2341 agttggcatt tggtcatttt tctccttctt tgagagtgag agtgcgtact ttggtccttg
     2401 agtcttctca aagatagggg caccgagtct tctagctaac ttgtattttg gtccaatttt
     2461 cataaaaatt tattatgctc gacgaggctt aggtggctta ggaccgttgt gtggcacggg
     2521 tgtcctgtcg gtaatagtag taagattgat accctttgaa ataaagcctc tgatggctga
     2581 ctcacggcct gagccaacac cgcggacatt cacactcacc tccttaacac ccagtagaag
     2641 tgctttatct ccgataactt ctccaatctt ggctgctgcg aaaggagttc ccttcttagc
     2701 gcccttgaag cctagtgccc cagaggtcga ccaagcgacg acattgccct tctcatcagc
     2761 aagaacaacc ttggtgttgt tataagttga ctcgacattg aggatcccag tttctaggcg
     2821 cttcttgcca ctagtcttag gctgggccgc tactgctggc tttgtctcct ccttgccttc
     2881 tgtttctata attcgttttt tacccatata attttatgtc ttttctactg cacgacgacc
     2941 agtacccatg gtctttcgta cgttacctct tcgagtacga gagttagtct tagtcctttg
     3001 acctcttact ggcatacctc tctggtgcct aacacctctg taggccttga tgtccttaag
     3061 tctcttgatg tttgcctgaa cttcacgctt taggtcaccc tcgagtgtaa tcttttctat
     3121 aatagatctg atcttgtttt cctcctcaat atttatatcc ttgggtctct ttgagctatc
     3181 aaccttagcc tcagagagga tagcggctgc acgagatcta ccgatgccaa aaaggatcgt
     3241 taatccaata tcaatgcgct tatcttctgg tatggtaatt ccgagtaatc tcatttaacc
     3301 ttgtcgctgc ttgtgtctag cgttagcctt gcaaataaca tacacgtatc ccttgcgcct
     3361 aacaatctgg cactgaccgc acattttctt gacagacgcc tttactttca tagagaaaac
     3421 acattgtaac cgagtgcttg tttcttgtca acaacggctc tacagacgct taataatgcg
     3481 gggcttacct ccatagggct ctaattcaag gataaccctg tcgccgacaa gaaccctgat
     3541 tctatgaagc ctcatcttgc cagacaggta ggtcaaaatc tcctcgtctg acccatcaat
     3601 cttcacctta aacatgatgt ttggtaaggc ttcggtgact gtccctgttc tttctagctc
     3661 tgggttatgg ggcatttatt tttgaacctc tatcttaatc tttgtttcgt cagttgggaa
     3721 gctcgatttc cagaatggtc taatgactgc agacgcctca acaattgatg ggtaaccctt
     3781 tagtatctca aacaactcag attcctcctt accctttata tcagacatta gagaggcttc
     3841 gtcagtaaca gagataagag ttgcagaacc gataaacgaa agcttaatag cttgggtact
     3901 tgtggcttca gtatttacaa gtgaagcgct aagcttgctt gtgtccaaga agtcgactgg
     3961 agcacctacc ttatcagccc ccaacttttg caccgccaag tattgagcta agtcagtttt
     4021 cttaaagatc caagcactta ggtcgcctct taatgtaacc tttgccttac cttggtcccc
     4081 cgcctctgta ggtaaaagct cataagtaac cttgtttagt ccagggaaaa gtatgtaacc
     4141 ctctggtgtc tgagcttgga tttgtgcgtc aagatctgcc ttggcctcgg attctagtgc
     4201 tgtttttgca gtattaagag tatcttggct caccttcttt ctcaaaccga tgaatccgcc
     4261 agtcatatta gtcttagatc taccataaac ttttgcaaat ctagccgtac ccttgagacc
     4321 cggcagagta aagtcagaaa gtgtaatgtt gtactctttg cccggttggt ctgcaataac
     4381 ttctacctct aagttgctct tcgccttcac tgttacatcc tcttgcaacc taaacacttt
     4441 gccatcagtg gattcgaaac gagtactctt aaccaattgt tggtctgttg tttgctcgtt
     4501 atatattgca attcgaccct tagacttctc tgaaacagtt tcgtctcctg cagcttctac
     4561 atccaaagac tttgtacttg aaagtttgat agtgctgtag accaatgcac tagtactggc
     4621 gtttggatag gcggaataag tgtcgttagc gaaggcaact tgagtaacac gaggcttata
     4681 ggtaagaact gcaccattct ttaaagtgat aaacattaaa acaataagaa ctactacaag
     4741 tatccctcct attattcttt tagccccgga aaaattattc ttaacaggct ctacgtaact
     4801 aagtcgaggc tctgatctaa ttacctgagg agtgctcatg gctgtgccca ctgacttttg
     4861 tggatttata aatgtctccc gcgcagtctc tctattgatc tctctagcta caggtctgac
     4921 atcatcagtc tttcttattg gtatgggtat atctcggata ctctttctat ttgaatgaac
     4981 tacatcttct attttctttg gcatttgagt aaattgtatc ttagctaatc aggtttgcaa
     5041 aggaggataa gagcaatatc ctctcatcct tggactcttt ggcactattt tctaccaatt
     5101 ccttggtaaa ggtgttggtg tcgccgataa gaataatgtt tttgtctgga aagacgcttt
     5161 ctaaaaaagt tttcatcaat ttgccaaact ttggatcagt gacaaggaag atggaataag
     5221 gcacctctgc tgtatgaatt attttttcct tcacctcagc ccataagtct ctccaaactg
     5281 tctcattctc tgtaaggatc ttgtctatcg actctgttgt gtctttgtct agcgcacctt
     5341 cggcaaagag tgttaggtaa gagcttgcaa cctcacttgg tatattcaac tttcctgcca
     5401 ctgcttcgat aattgatgct ggaccgaagg gtaaattcgc attcaacgcc aaattattat
     5461 gccttaggaa caagacatca gtaatatgag tcgtcatatt gataagcagg gctgagtgta
     5521 ggttattgaa ggagtgcgag agcactttgt agaacacata cataaagctg tgcattacca
     5581 atccttttct caaagctaag gtcttaataa tctggtcctc aattcttgtc aaaagatcct
     5641 tggtcgaagc actaagaatg aaactgatct ctacttcgtc tacactttcc ttttttggct
     5701 ctgaagctgg gtaaccgttc aaataaaggt ccataacccc cgcttcaaaa atctcactct
     5761 cctcggagaa actctccctt aagttgtttt gaaaaatact tttttcctca ttgattacac
     5821 caagcataaa tttttcgtcg aaaacgaact ctttctcctt tttgatctct acagtcttca
     5881 aatatgagtc gacccaagga gaggagaagg tgataacagc cttatctact ctgaccttct
     5941 tcattgtaga aatttttttt acgataaaat caagaacgat gccgagagag ttaagcatgg
     6001 ctttgtctgg cgagaaggca ctgtccacaa ccttgaagtt gtactcggac tctatagcag
     6061 ttaggagtct gggtacttta cccttacttg tagcgactag agcaccggat gtagaaccgg
     6121 aggagatgtc tattactaga atgttaaaat cctttttccc actaaaccaa agtgacataa
     6181 ctctattgta gcaaagctac attataaaag cgttgctttg atgcgcctaa ctccagctga
     6241 tactgcctcc tccttatcaa tcttgaactt accaatctcg cctgtgttct taacatgtgg
     6301 tccaccacaa aactcttttg agtaatctcc aatcttatat acagagactt ggtcagggta
     6361 cttctggtcg aagaaatgca gagcacccat ctttaccgcc tcgtctttgc ttgtaacaat
     6421 attcgtcaca tcaagcttct cttctatttt tgtattaact attttctcaa cctgcttttt
     6481 ttcttcatct gtcatctttc gagagaatga gaagtcaaaa cgcaatcttt cgggagtaat
     6541 gtttgaaccc ttttgactta cttcgttacc taaaacgtct cgaagtgctt gatgcaaaag
     6601 gtgtgtggct gtgtggtacc taacttcgat ctccgagtga ccagcaagcc ctcctttaaa
     6661 cttttgttct gcaccagcac gcgagagttc ttggtgcttt tggaactttt gcttgaagtc
     6721 ctcttcgtta atctcaacac ccctgtcatt cgcaagctct ttggtaagtt caaaaggaaa
     6781 tccataagtg gtgaagaggt caaaggggtc agcccctttc tcaaattgtt tcaatccatc
     6841 cttcagagtc tctttaaact tacctatttc tgcatcaatg acattattag ttaaatccct
     6901 atttaaagtg taagaactat aaacaggatg gtcaattata gcttctgcta ctttgatcag
     6961 gttaattgaa tccaggccta acttttgtgc gtggataaat gatcttctta gcaaccttct
     7021 caaaatataa ccctgatctg tgtttgaagg cactactccg tcagaaatca taaacacaga
     7081 agctcttatg tgatctgcga tgattcttgc tgatacattg tcggatgttt tgccaagtgc
     7141 gttaaatatc ggttcaaata tgtctgtgtc atatgcagtt ttcttgcctt gcataacagc
     7201 tgtcatacgc tctagaccag cacctgtatc cacattttta ctcttaagct tctcaacgac
     7261 tttgccattc tcctttctaa actccatgaa gacgtcgttc caaatctcga ccaaatcctc
     7321 tcgctttact gccttaagaa attcatcttt gttcaaatct cccaaacctt ctgttagatc
     7381 ataaaacatc tcagtatctg ggccacaagg gccattctcc ccagcactcc accagttgtc
     7441 ctctgctcct aacgcataaa tacggtgctc agggacacct aaactcatcc aaatcttctt
     7501 cgactcctca tcgaaaggag catcatcatt accttcgaac acggtgacat atagcttggt
     7561 tggatctagc ccaagacctt cgactttcga ggtgagaaac tcatagctcc acttgatagc
     7621 atcttctttg aagtagtcac caagagacca gttaccaagc atttcaaaga aggtgaagtg
     7681 tctgttatcc cccacatcat ctaggtcgcc tgtacgaaca catttttgta catcgaccag
     7741 cctcttaccc ttaggatgct cctcaccaag gaggtatggc actagtgggt gcatacccgc
     7801 agtggtgaaa agtactgatg catcgttctc tggtataagc gatgcgctag ggatcacagc
     7861 gtgtccccgc ttctcaaaaa aagatagaaa ccgtcttctt atctcggttg attccatggc
     7921 gctaatatat cactaaatca aggttttcag aactttgaag tcatctaaca acactggcct
     7981 tagcttttgg ttatatatag ccgcagctgg gtggaagagt gggactaatt taaacccatt
     8041 tggagatttt aggacctgac cgtgaatgtc agatattgcc ttgatctcct cacttagccc
     8101 atagcgtttc ataacatact ccatagagaa tctaccaagt gtggcaatnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt agcccatagc gtttcataac atactccata
     8281 gagaatctac caagtgtggc aatgaccgcg ggcttcattg tttcgatctg ctcatctaag
     8341 aacggtccgt acaactcgat ttcttcaggt ttgggatctc tattttttgg aggcctatct
     8401 tttacaatat tagtaatgta aacactttct ctatcgatac caacggactc aagtaactca
     8461 tttaacactt gtcctgcacg accaacaaag ggtttacctg ttttcgcctc cttatctcca
     8521 ggtgcctcac ctataaacat tatttttgga gtggtgctac cctctcctat tacaggtaaa
     8581 tagtccccgg ccctacgcaa gtcggccaac tctccatcca tagatatgac cttctcctcc
     8641 atgtttaaaa tggttcaaac tgtgtctcct ctgcattctc aaaagtcatc ttcttgtaga
     8701 ctgctctagt tgcttccaca tccctcgcgc agtactcaca tatttcttta attttacccg
     8761 ctttgtagaa gttccaaacc tgcgaaccgt ctatgccctc ttttggagta ggaacgccca
     8821 tggcataaca tagatgctct aaagactttt tgtatccaga ccaattatcc cattcgtgca
     8881 ttgtgtcaaa aattggcgca ttcttgtatc tggcaaatga tagaaatttc accatatccc
     8941 aaggtttttt gccaggctct tcaaatttat tccaagtagg tttaactcct aagatcgctg
     9001 atctctgcat tatgaatggt aggtcaaaat cacgaatatt gtgtccaatt attaaatcaa
     9061 cctttcccgc aatctcccaa aattttttaa ccatttgctt ctcatctcca gacaaatatt
     9121 cagctggttt gtcatttatt gcataggcaa tacaacaaat tctaccgtac gctccatcga
     9181 atgaagtctt ctcaacaaat ttctcgaaag tatcttgaat cttgtcagaa tcaggttcta
     9241 ctttggatat ttttttatca taaagatact ttaaggcctc catcgattcc tcactcgcgg
     9301 gaatcgtctc aatatcaaaa aaaagtttag tcacgtcaat ttttcctagt gatcaaatgt
     9361 tccactgtcc aaaggagtgt aaaaacaata agagtaatga aggtggtgaa aatagccagt
     9421 cctacttgac caaaaccaac cgccatacca ataccagccg agacccaaag acccgcagct
     9481 gtcgtaagac ctgtaagctc gtggttctgg aatatgatca ctcctccaca gagaaagcct
     9541 atacccataa tgacaccagc cgcagtacgc attgggtcta agttgaccat accactgtct
     9601 gcaagaacga gttgagagag tataacaaag aggcatgagc ccatagatac gagaccatat
     9661 gtacgcatcc cagccgtctt gtgggcaata gttctctcaa gtccgattat taccccaagg
     9721 gtgagagcta tcaaaagatg ccaatactgt ggtgctaatg cttcagtcat tgtaattgtt
     9781 ctatatgttt tttaaatatt aaatcgagca actctggatt ctcttctatc atgtcttgca
     9841 agttacctct ctctggatag ttccatccta tcatggtgag cttttgacaa agtccataat
     9901 gaagcgctgt tgaggaaaac tttgtgttag ttataagcca tccctcatcc agcttcctat
     9961 ctctaccacc ataatggtgc atattatcct tcaagtcatc aaagcgtgcc ttaacataaa
    10021 gagccacctt gagatctgat tttattccca ggccgttgtg gaacttcgct tcaaccataa
    10081 caagtttgtc atcgttccat gccacgacat ccatctcgtg cggcacacag ccgccgagca
    10141 ctgtttgccg ggttaaagtt tcaaagccct tggtcctaag cacctcagct acaaagtcct
    10201 caaatgggaa cccagttggt cccatatcaa gcattgctcg tcttacagag tatctttgcg
    10261 ccacaggctt ctgcatctct acaagatgtt tgaaggcgct cttgtatatc tctcttgttg
    10321 tcatcccctc gcggagctca ctcatcactt gagctattac tgcatctgct gcttcctcac
    10381 tggctctagc tctaataagt gaggttctaa gttttgatgg gttgaagatc tctcttgtcc
    10441 catcggcctt aataatgtca gtcatttagg caattatagc accaccaagg cacctctctc
    10501 cagagtaaaa tactatggac tgacccgaat caaccgtctg aggttctagg aactgtacaa
    10561 tgttattttc tagactcact ttaaactttg gttgtctgta cctaatcctt gcttctatat
    10621 ctactggcaa tttcatggcg acaagattta atctctccaa attcacttcc tctgacttac
    10681 tctttagtat ttcctcatta tcattcgata cagttatagt gtttttctct atattcttac
    10741 taataacata ataaggttta gagtcagaga ctttctccgt tatttcgaat ccatgtctct
    10801 cgcccaaagt atagtagaga gcgccagtgt gcgagccaat aatattgccc ttagtatcaa
    10861 gcacttcccc agacttagtt tctatataat gcgagaggaa atcctcgata tccacatcgc
    10921 cgatgaagca tataccctgg ctatccttct tctccgcagt atgtagaccg tgtttcttgg
    10981 caatctttct tacttcatgt ttcttcaagt gacctatggg gaagagcacg tgttccaaat
    11041 catctttggt caaagtccaa agaaagtaac tttgatcttt ttctttatct acaccttcat
    11101 ataattcgtt atcaatattt ctagcatagt gacccgtagc gatgtaatca gcacccattt
    11161 ctttggctct atcccaaaag gcgccaaact tcacctctct attacacata acatctgggt
    11221 tcggggtctt accctttcta tactcttcga tcatataatc tgcaacgccg ttcttgtact
    11281 ctttttcaaa gtcgaaaaat aggaagggta tatcgagctt cgctgccact ctcatagcat
    11341 cacgtctttc atctctccag gtacatggca aaaagtcagg ttgccagacc ttgataaaaa
    11401 ctccagtgac gtcgtatccc tcttctttaa gcagtgcagc cgaaacagct gaatctacgc
    11461 caccagaaag tcccacaaaa acctttttag aggtatttct tgatattttg gatgtgttca
    11521 tcaagtgtcc tagcataatg tgtcttgcct tctttgtcac tgatgaagta taggtatgga
    11581 gagctcttgg ggctaagagc agcttcaata gacacgagtc ctgggttatt aattggtttc
    11641 tttggtaggc caggctcgtc ataagtaata ggagcggaat cgacctgaag ggccatacct
    11701 attgagagtc gcttccaaag aatacctgag actaactctt tatcttcttt tgtttgcacc
    11761 tctccttcga taagcgaagc catagtaatt atttcttctt tgctgtattt agaagcatca
    11821 aatttatcct caaataaaag tgttttcgac tcaaaattcg agttaagtat ttccaccact
    11881 ttctccgcag tgacactcac cttcataaaa taagtgtcgg ggaagagatg tccctgctcg
    11941 cccaaatcaa gaaacatctt cttattaaaa agtgggaagc gctcatcaaa caggcttgca
    12001 atctctgtgt tatcaaaacc ctctggtatc gtaatcttaa cttcatctat attgtgctct
    12061 ccgcgaacca ttcgccacgc aagtcttatt acactctctc tataatgcaa ataatactcc
    12121 cctgccttaa ctccagattg tgtaccaaat agggtgacaa aagtgttgaa aaggactctc
    12181 gaacgaatta cattagaatt cttcaaaaca tcactcactt ggtttaggct gagtcctctc
    12241 tcaatattca aaatactctc ttccttaaat gcatttggtg gagagataat gtgggcaaag
    12301 aataatacaa acaaaaaaag acccacgacc aaatatattt ctttgtctcg gaaaatgtct
    12361 ttcatcaaat aggtagattt gccggagcct cggccttctt tgaaggaata cgcgtaagag
    12421 ttggtgtgcc agcaacctca ccaggaaagt ggaagatagt agcaatctcc tcagttgtaa
    12481 ggataaatgg tttgccacca atatgcttga atggtagctc gaagtaagaa cgtctcttat
    12541 acgcctcaag taactctctt tcgttattgg ccttcttttt accaaaagga tcttgccagg
    12601 gataatcgta accagtacca aaacttggtc tgaagccgtt aagtgtcgct gacccaaatt
    12661 gagttataga tccaataatg ccgccaatat ttgctgggtt aaaggcatcc ttctcagcga
    12721 gataagttgc gcgaatagct gtgtcgaagg cgagtttgcc agtagttctc tcaatagctt
    12781 tgaccaaatc cttctgacct tcagacatgt gctggccgct aatgttcttc tcctcggctg
    12841 acttgaacct accctcctta agtatcttag caatttcttt ttcgatatct ttcttccagt
    12901 ctggtttcac cactatgcga ccaagcttaa gaccttcctt ggcatgtcca cggattagta
    12961 tttgtatcca agcttgctca cctcgcttga gtgaccccaa atactcaata acagaaacca
    13021 ttggatcaac cttgaactcc tccttaggat ctttatccaa tccataatca atataagtct
    13081 tgattggata tgcatcgggt ttagtaagag taaattgccc aaagtaacct atactcatct
    13141 tctctggatc tctcttcact cccagtgaat aatcctctgc ctcatgaatc tcgatgttgg
    13201 ggaactgggc atatagttga gcctccaccc tagctctaaa actcttatgc atccatataa
    13261 aaaatttaac agctccacca atggatacaa gctcgagaga gaaccacggt ctaacacgac
    13321 ccttaagata cacatctata agtgtcccag cacttgtttg gtgcaggtga tttaagaaga
    13381 gctccatcgc aagaggcgac ttcataagct ctctcggaag tttaatctcg aggagtacac
    13441 taccctgagc agcaacccac tctcgtctct tcaaattcat ccaaatttga aaagctacac
    13501 ttataaagat aataggtatc cacacaggag atgtgacata caagatgtgt gcgagttgta
    13561 agtagaattc tttacttgtg agaagtgcga agaacgcgtc tagcattctt ttattttacc
    13621 ctaaattagg caatgttgta acgaccctcc ttgtctttct tgaaatactt tgggttttga
    13681 agattaacca taatggtatt ctccttaaca taacgctcct taagtacctt cttgattatt
    13741 tcctccttag tgagagggcc attcttatca ataatcttct tgataacatc ttttacaact
    13801 ccggacatat aaccccattc agcaagcgca taaaggcctc gacctacgag gacgaaacga
    13861 ggatccttga taagttcgtt gtgtgtagta gctacatgag cctttttatt gaaatattgt
    13921 gagatcgctt tggccacttc cttgaagtgt attggtgagc cgtgctttct aataacaagg
    13981 aaagcgtagt cacgcattcc cttggcatta acatttggag aatctgttcg accccactca
    14041 cctagaggat ttttgccgat cttatttgag atagatagcc aacgctttat aacctcctcg
    14101 tttttatact tatcattcat gtccttaact tcctctagga agtcgttgat aagatcacct
    14161 tcaagtacaa gctcatcgtc agagagcttg gagtaaagct tgcgaagtga tgcatggata
    14221 cgcttagaaa gatctgagtc aacatgccat ctgtgcttga actcttcgtc ctctttctct
    14281 cgcatgaact cctcgccaac tactagcagg aagtggatat ggttctgacc agactcatcc
    14341 ttagctagat gctttagaag atccttctca gacaccagac ctccaagttc gaccacgacg
    14401 gccttaagct catcaaagac tacttgctcg gacttgtact ccttggactt tctgattaat
    14461 gagagggagt ggttctctat ttgtctgact ctttcacgag taataccgta cttttttccg
    14521 atcgcatcaa gtgtcatgcg caccgatttt gcaccaagac cgtaacgatt ttcgactaca
    14581 tctgctcctc ttttattgag aacagaaaga aggcgcttgg tgaccgcctt tggtttgaag
    14641 cttaatgtaa tcttttcgct catatagctt tatctatttg tatcaccccc ttgacatcat
    14701 gtcaagctac cacaatattc acaactttac ctggtacgac tatcactttt ttgacctcct
    14761 taccctctag ccatttagac acagcttcgt ttgatagggc caacttctca atctcaatct
    14821 gatcctggtc ttttgatact gatatctcac tcctgacttt accgtttatt tgtatagcat
    14881 aaacaatctc gctctttacc agcttggact catcataagt gggccacgat gcagtatgca
    14941 ctgactcttt gtggccaagg atactccaaa gttcttcagc gacatgtgga gcaaacggag
    15001 cgaaaagaga tacaagtgtc tcatattcct ctttcgatat agaggtcttc ttgtctaatt
    15061 cattcaccca gaccataagt gcagagacag ctgtgttgaa acccatacct tcaatatcta
    15121 gggttacttt ctttattgca gagtgcatag agtagttatc tgtctcaaca cctccaactt
    15181 tgttgccaag cttccatact ctttcaataa accttcttgg tccgactaaa cttgcctcac
    15241 tccaggcaat agcttgttca aatgggccca taaacatttc ataaacacga agggtgtctg
    15301 caccaaactg ctcaataacc atatctgggt taaccacatt gcctctggac ttactcatct
    15361 tctctccatc actcgcaagg ataataccgt gcgatgtacg tttcttgtat ggctcgcttg
    15421 taggcacaag accgatgtcg aagaggaatt tgttccaaaa acgagagtaa agaaggtgga
    15481 gtgttgtgtg ctccatacca ccgttatacc aatcaactgg agaccaatat ttaagtgcat
    15541 ctttggaagc aaatgactca ccgttctttg gatccatgta acggagaaag taccatgatg
    15601 atcctgccca gttaggcata gtatctgtct ctcttttagc gtcccctcca cactttggac
    15661 actcggtatt cacccaagaa tcaatagtcg ctaagggtga ctctccggta tcagtgggtt
    15721 gataattagc aacctcgggc aataacactg gtaagtcgct ctctggcaca ggcactgctc
    15781 cacacttggc acaatgtata acaggtattg gctctcccca gtatctctgg cgagagaata
    15841 cccagtcacg aagcttgtat ttgataacct tgttgccaaa gtcttcgaac ccagccttct
    15901 tatagtcagt aacaataggt aaatcatgct tcttggcaaa gaccttatct ctctcatcat
    15961 gtgctggcac acccatgatg gcaccagtgc cgtagtcaga c
//
LOCUS       DAORIT010000017        13160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_801_length_13160_cov_52.358565, whole genome shotgun sequence.
ACCESSION   DAORIT010000017 DAORIT010000000
VERSION     DAORIT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_801_length_13160_cov_52.358565"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..911
                     /locus_tag="PLD99_02135"
     CDS             45..911
                     /locus_tag="PLD99_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRH25734.1"
                     /translation="MNTEAKTLKQWIAWAEEKGVAIGHFNVSDSEGFNAVVDAAKELQ
                     VPVIIGVSEKERSFLGVAEIRALVQVEKDKGLPVYLNADHTYSVEASKEAVDNKFDEV
                     IIDGAEKPFFENVMMTKEVVQYARENCPGMLVEGELGFIGSGSMLRDEIPDGVSVETQ
                     TSVEDAVKFVEETQVDLFAPSVGNIHGLIKSGEPELNIKRIKEIAEAVKIPLVLHGAS
                     GNTDEEVGKAIDAGIRIVHINTELRQAYKKGLEKGMSTDELAPYKFMAEGVEEMKKLV
                     IQKLKVFNKMNA"
     gene            904..2538
                     /locus_tag="PLD99_02140"
     CDS             904..2538
                     /locus_tag="PLD99_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRH25735.1"
                     /translation="MLKEEIKKFFKGDVLDDEETLIKYSKDASLFLVRPKIVVYPKDS
                     EDIRNLVKWVGENKESNPTLSITVRAAGSCMSGGPLNESIIVDVMKYMNKVNTIDNNE
                     VVTETGVYYRDFEPKTLEKGLILPCYTASKNLNAIGGMIGNNSAGEKTLRYGKMENYV
                     LESKVIFADGNEYVVSPLEKTELDKKMKQKDFEGLIYKKLFNLIETNKQIIDEAEPKV
                     SKNSAGYTLWNVWDGNKFDLNKLLVGSQGTLGITTEARLKLVKVEPKSRLFVIFLPNL
                     SHLAELINDVLPTNPDSVETYDDATMKLAFRFFPEMLRTMKAKHFFKLIFSFIPEALM
                     MLRGGVPKLIVLVEYTGTTDEEIEAKMQDLKSKIAHLHFVERETRSEEESNKYWTIRR
                     ESFNLLRKHIKGLRTAPFIDDVVVKPEHMPEFLPRMREILDSYKMVYTIAGHAGNGNF
                     HVIPLMDMKNRDNVRVIKEVGEKVYNLVKEFNGSITGEHNDGIVRTPYLDKMYSSEVL
                     KLFKETKDIFDPQNVLNPGKKVNGTIEYMENHLAIE"
     gene            2585..3232
                     /locus_tag="PLD99_02145"
     CDS             2585..3232
                     /locus_tag="PLD99_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRH25736.1"
                     /translation="MTKLKVELRDKSVKLDLLRKEGRLPAVFYGKKEASTPISVSKID
                     FLKAWKEAGESTVINLETPNGVVESLIHDVDFDPVTGNPRHADFYVFEKGHKVKIDIP
                     LEFVGVSAAVKDLGGILVKVMHEVKVEAMPKDLPHNIEVDISSLLTFDSQILAKDLNL
                     PTGVELIENGEEVVALVSAPREEKEEESKPIDLAAIEVEKKGKEETEEAPAEESK"
     gene            3280..4632
                     /locus_tag="PLD99_02150"
     CDS             3280..4632
                     /locus_tag="PLD99_02150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRH25737.1"
                     /translation="MFFVSLVVGGSFFVTGFRVAQAQTDPCNGNIVGKTNSQLQADLE
                     ACNAEIAKWTDVLNSTKKNTASYATEVSKLTAKINAAQATIKSKTIAITNLNNNISKK
                     QSEITDLETQIQNDIDHIAEIIRKTNQIDSYSVVESLFSDKDLSEFFVDVDTYASTRS
                     ALKTVLDNLRGVKSVTEEEKAKLAKERDLQAAAKAEIEANKRKVEVDQKTQKTLLTQS
                     QNQEKAFASLVAEKQAKAAAIRTALFGLRDSAAIPFGTALAYAQDAGAKTGVRPALIL
                     GILQQESNLGANVGTCVITDLSSGQTKSVNSGKVFSNGIHPTRDLPLLQSLLPALGRD
                     PLVTKVSCPLSIGYGGAMGPAQFIPSTWNIMRSRIATALSKETPDPWNPADAIMAMAL
                     FLKDLGAGSQTYTAERNAACRYYGGGTSCNTRTSGYGNSVMSRATNIQNNMIDLLQDA
                     "
     gene            4656..4919
                     /locus_tag="PLD99_02155"
     CDS             4656..4919
                     /locus_tag="PLD99_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRH25738.1"
                     /translation="MKKDIHAKVYRPVLFKDSGNDHEYIISSTVETKDEGKAKDGKTY
                     PLFRIEISSSSHPFYTGQEKVLDSAGRVERFKQKQAKAGTTKK"
     gene            5031..5981
                     /locus_tag="PLD99_02160"
     CDS             5031..5981
                     /locus_tag="PLD99_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRH25739.1"
                     /translation="MDIQKYKDNPKTLYLAESFERLLKEEEETRAMAGADAEMAKLAE
                     EELNNIEIQKKELLTQMDEILKAEEKEEEFPNEIILEVRAGAGGEEAALFAKELADMY
                     RAYAKLQGWGVQVVDESFSSLSGYKEASFEVRGMDVYKKLRYETGVHRIQRVPDTEKT
                     GRVHTSTASVAVLPVRKKSKIEIKPTDLEIETSRSGGAGGQNVNKVETAVRIIHKPTG
                     IDVRSTSQRNQLKNKEMAMSILMAKLEAKKQEEEDAKYSADRKSQVGTADRSEKIRTY
                     NILQDRITDHRINESWHNIQKIFQGYMEPIFESLEAEANK"
     gene            6457..7170
                     /gene="rpsB"
                     /locus_tag="PLD99_02165"
     CDS             6457..7170
                     /gene="rpsB"
                     /locus_tag="PLD99_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRH25740.1"
                     /translation="MTTKTSNNSNLIDELFQAGAHFGFVKSRRHPSAKPFIFGSKNDV
                     EIFDLEKTSEELLKVLAFVEEKGKEGAQGLFVGGKSEAREAIRKAGTELAQPFVDGRF
                     IGGTMTNWPEIKKRIAMLEDLTTKREKGELGHYTKKERLLIDRKIDKLTLYFSGLSTM
                     KGLPKFLIVVDPKKEHIAVAEAKRIKIPVIAIAGSDNNIFDVDFAIPANDSSKASIGL
                     IMEKIVLAYKTGAASVKVA"
     gene            7194..7655
                     /locus_tag="PLD99_02170"
     CDS             7194..7655
                     /locus_tag="PLD99_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HRH25741.1"
                     /translation="MADITTADIKALRDETGLSIAEVKKALDESEGDKAKALEVLKSR
                     SAEVAAKKGDRALGAGVVNAYIHNGGASGTLLELSCETDFVAKNEEFKALARDIAMHV
                     AAMNPADVAELMPQPFIKSPDMTIQDMVNGATQKFGERIEVRRFVKLSVSE"
     gene            7678..>8033
                     /locus_tag="PLD99_02175"
     CDS             7678..>8033
                     /locus_tag="PLD99_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25742.1"
                     /translation="MILNIIFIISLLGIVTLIISKILEEKSKGKPLFLRLVSLGDERV
                     RNATVEFAHMYSDWRERGHFFLNNQLPLHTKNLMNKFSLMIKDKFEKHVGDIRGSKFL
                     KKSDGLSEYLKSISEK"
     assembly_gap    8034..8133
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8134..8209
                     /locus_tag="PLD99_02180"
     tRNA            8134..8209
                     /locus_tag="PLD99_02180"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8167..8169,aa:Thr,seq:tgt)
     gene            8173..9396
                     /locus_tag="PLD99_02185"
     CDS             8173..9396
                     /locus_tag="PLD99_02185"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRH25743.1"
                     /translation="MKVLGSNPRGGSKEVTNNHKIYFENLDSLRFLAFFVVFVSHAAL
                     FLGFTSNSSLYQSIKYNILVNGDLGVTFFFVLSGFLITFLLLKEREVSGGISLKKFYL
                     RRILRIWPVYFVVLLMGYFLIPYLVQLFGSPSLPIDALPPQSALPWHTFFITNFAMAR
                     SFFSSVPTDILWSVGIEEQFYLLWPLLVILTPRRMLSRVVGLIVFAAFIFRGYFAHEP
                     DVLAYSTFSAMSDLGIGCLLAIFVLSEYVSRLRAMFSKVTTWLLYILVFALTISRHGL
                     VSFFSDYEHVFSIFVPVLYLFVSILFALIIFEQNESNNSIFKAGKFPLMSGLGRISYG
                     LYAYHPAVYVFVLLIMTKIGSATSYSSPFKFLVVSIAVLISTIIFSKISYAVIEKWFL
                     KRKPSSLTSSDSFSL"
     gene            complement(9397..10095)
                     /locus_tag="PLD99_02190"
     CDS             complement(9397..10095)
                     /locus_tag="PLD99_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRH25744.1"
                     /translation="MPDSKKYIFAFVMTVAIFATALFISNSVSEGKLAEIQNIQNRLS
                     LDILASETQSALLEETSCKNVNQIPLTKELGELAERLAAAEEQSGSDNQDVQSLKKQY
                     FLLEIRDYLLMKKISEKCGVNPTFILYFYANETDCEDCQKMGFVLTALHEEFPDLRIY
                     SFDYNMDLEALHTLRTIYGLGETLPAIVINGDPYYGFRPLNELVESVPALSRLRAEQI
                     KQQKKATTTKSLGL"
     gene            complement(10104..11354)
                     /locus_tag="PLD99_02195"
     CDS             complement(10104..11354)
                     /locus_tag="PLD99_02195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRH25745.1"
                     /translation="MAISSAILYISLFVSLFFEIFLLITYFEVKSEIDFEIEHGGKDI
                     KSFPTVTIVVPCHNEERTVVDTIKSILSLDYPKDKLRLMVIDDGSTDRTKAVVLETFR
                     GTPQVEVFSKENGGKHTALNFALEKIDSDLVGCLDADSFVEPTALKRIVTFFDKEEVM
                     AVTPSIRIHDPKTPLQYMQRVEYSWGIFFRRLLSSINGLYVTPGPFSIFRTKVFKTIG
                     GYKHAHHTEDMEMALRMQKNGYKIVNAHSAHVFTVGPDKLKKLYKQRVRWIYGFLNNA
                     IDYKEMFFNKKYGNIGMFVLPIATFSIFTTLYAAGNLLWSIASKIPEHLAKYEAVGFN
                     LGMPNFSKINWFAFDLNTSFWITVTTLSLTFVILYLSLRVANGKARMGRDVFYYLTLY
                     AFIVPLWLAKATYSTITQKKISWK"
     gene            11408..12559
                     /locus_tag="PLD99_02200"
     CDS             11408..12559
                     /locus_tag="PLD99_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25746.1"
                     /translation="MKNLYKVSLEEQNKYLQILESRTDSEALRMKRYLAMKDLSRTEG
                     SPLKEMVENILGQETFKDFDVIETPEVVSTKIGFDLFDFPADHPARSKSDTYYVDDNS
                     FLRTQTTVMWYYYLQEKDVQERMARGEAVGCFSHGKVYRKDEIDRHHMNVFHQLDGWY
                     LAPKEKETIGEEDLKNVLNSIAKAVYGDSAITRFNPDTFPYTHPSLEMEIDKDGKWVE
                     LLGSGVVKGSVLEKFGIDSTKWTGWAFGFGLERLAIVSMELPDIRLLWSDDERVAKQL
                     VLGNKYKEVSKFPPVVRDISFVVDKSSFVLNDYFDAIRDIGGEMVEEVALIDKYENAA
                     KFGEGKMSYTWRVTYRDLNKTLTNEEVNALHAKVEEMTVKDYKGVIRVS"
     gene            12556..13137
                     /locus_tag="PLD99_02205"
     CDS             12556..13137
                     /locus_tag="PLD99_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRH25747.1"
                     /translation="MSYLVGLSKDEISEDTLNKSGLKWKKVNPRETILSIAPSLLGVP
                     YKFGASVSKDSPNAFDCSSLTAYLYTQGGVAIPRVCDDQYKAGDEVPETEALPGDLVF
                     YKGNRTDISADKVGHVGVYMGEGDIIHAGGVSIGYGKVVREKITESKYYPSGFLGYRR
                     LLQSGDDRFVVEIPEDRPELRDRNILIKELQSL"
BASE COUNT         3797 a         2511 c         3263 g         3489 t
ORIGIN      
        1 atgtctccca cagcgataaa atcatgcatg atacaattat acccatgaat acagaagcaa
       61 agactttaaa gcagtggata gcatgggcgg aagagaaggg tgtggcgata gggcacttca
      121 acgtttcgga ctcggaggga ttcaatgcgg tggttgatgc agcgaaggag ttacaagtgc
      181 ctgtaattat tggagtgtca gagaaggaga ggagtttcct tggagttgca gaaattcgcg
      241 cacttgtaca agtggagaag gataaggggt tgcctgtcta tttgaatgct gaccacactt
      301 attcagttga ggcttcaaag gaggcagtcg ataacaagtt tgatgaggtg attatcgacg
      361 gtgcggagaa gccattcttt gagaatgtca tgatgaccaa agaagtggtg caatatgcga
      421 gagagaattg tcctgggatg cttgtagagg gtgaactcgg ctttattgga tctggctcaa
      481 tgcttcgtga cgaaatacca gatggtgtgt ctgtagagac ccaaacctct gtcgaagatg
      541 ctgttaaatt cgtagaagag actcaggtgg acctttttgc tccatcggtt ggtaacatcc
      601 acggtcttat caagagtgga gagccagagc tcaacatcaa aagaattaag gagatcgccg
      661 aagcggtgaa gatccctctt gtactacacg gtgcatcggg gaacactgat gaagaagttg
      721 gtaaggctat agacgctggt atacgcattg tacacatcaa cactgagctt cgtcaggctt
      781 ataagaaggg tttggagaaa ggtatgtcga ctgacgaatt ggcaccatac aaatttatgg
      841 cagaaggagt agaagaaatg aagaaattgg taatacaaaa attaaaagtt ttcaacaaaa
      901 tgaatgctta aggaggaaat aaaaaagttt tttaagggag atgttttaga tgacgaggag
      961 acccttatca aatacagcaa ggacgcatcg ctcttcttgg tacgacctaa aatagttgtt
     1021 taccctaaag acagtgagga tataagaaac ttggttaagt gggtggggga gaacaaggag
     1081 agtaatccaa ccctctctat tactgtgcgt gctgctgggt cttgtatgtc tggtggtcct
     1141 ctcaatgagt ccatcatcgt ggatgtgatg aagtatatga ataaagttaa tacgatagat
     1201 aataacgaag tagtgactga gacgggtgtc tattatagag attttgagcc taagaccttg
     1261 gagaaggggc tcatactgcc ctgctacact gcttctaaga atctaaatgc tataggaggc
     1321 atgataggta acaactctgc gggagaaaag actttgagat acggcaaaat ggagaactat
     1381 gttctcgaat ccaaagtcat tttcgctgat ggtaacgaat atgtagtttc gccacttgag
     1441 aaaactgaac tagataagaa aatgaagcaa aaggactttg aaggattaat atacaaaaag
     1501 ttatttaact tgattgagac taataagcag ataatagacg aagccgaacc aaaagtttct
     1561 aaaaactctg caggttatac actttggaat gtttgggatg gtaataagtt tgacctaaac
     1621 aaactccttg tagggtcaca agggacgctg ggtattacca cagaggcaag gttgaagttg
     1681 gtgaaggtag aaccaaagtc taggctcttc gtcatattct tgcctaattt gagtcacttg
     1741 gccgagctta ttaacgatgt tttgcctacg aaccctgatt ctgttgagac atatgacgat
     1801 gcaacaatga agctcgcctt tcgattcttc cccgaaatgc ttcgtactat gaaggccaag
     1861 cacttcttca aactaatatt tagcttcatt ccagaggcgt tgatgatgct acgaggtggg
     1921 gtgccaaaac ttattgtgct cgtcgaatac actggcacca ctgatgaaga aatagaggct
     1981 aagatgcaag acttgaagag caagattgct catctacact tcgtagagcg agagacgagg
     2041 tcagaagagg agtctaacaa gtactggact attaggcgtg agagcttcaa cctacttagg
     2101 aaacatatca agggtctccg tacagcgccc ttcatcgacg atgtggtggt taagccagag
     2161 cacatgccgg agttcttgcc acggatgaga gagatcttgg acagctacaa aatggtatac
     2221 accattgcgg ggcatgcggg taatggtaac ttccacgtca ttcctcttat ggatatgaag
     2281 aatagagata atgtacgggt tattaaggaa gtgggggaga aggtctacaa tttggttaag
     2341 gagtttaatg gatcaattac tggagagcat aacgatggta ttgtgcgcac gccatactta
     2401 gacaagatgt attcaagtga ggttctaaaa ctattcaaag agaccaaaga tatattcgat
     2461 ccgcaaaatg tcttgaaccc aggtaagaag gtcaatggca cgatagagta tatggagaat
     2521 cacttagcga ttgaataggg taaatggggg ttgattttgg tctcattttg ggtaaaatgg
     2581 gggaatgaca aagctaaagg tagaactaag ggacaagagc gttaagctcg atctcttgag
     2641 aaaagaaggc agactccctg cagtttttta tggcaagaag gaggcaagta cccctatttc
     2701 agtctcaaag attgactttc ttaaggcttg gaaggaggcc ggagagtcga cggttatcaa
     2761 cctagagacc ccaaacggtg tagttgagag tcttatccac gatgtggatt tcgaccctgt
     2821 tacaggtaac ccaaggcatg ctgacttcta tgtgttcgag aagggtcata aggttaagat
     2881 agacatacct cttgagttcg taggcgtttc tgctgcagtt aaggatcttg gtggtatctt
     2941 ggttaaggtt atgcacgagg ttaaggttga ggctatgcca aaggatcttc ctcacaatat
     3001 cgaagtagat atttcttcac tccttacctt tgactctcaa atcttggcaa aggaccttaa
     3061 tctccctact ggagtagaac ttatagagaa cggtgaggag gtggtggccc tggtctcggc
     3121 tcctcgtgag gagaaggagg aggagtccaa gccaatagac ctcgcagcca ttgaagtaga
     3181 gaagaagggc aaggaagaaa cggaagaagc cccagccgag gaatcaaaat aatattcatt
     3241 ccgggatacc ggaggatttg tatgcacctt tttagacaaa tgttttttgt gtcacttgtg
     3301 gtcggaggct ctttcttcgt gactggattt cgtgttgccc aggctcaaac agacccttgt
     3361 aatggcaata tcgtaggcaa gaccaactca caactccagg ccgatctcga ggcatgtaat
     3421 gccgagatcg ccaagtggac agatgttttg aacagtacca agaagaacac agcttcgtat
     3481 gctactgagg tgtctaagct cacggccaag atcaatgccg ctcaagcaac tatcaaatcg
     3541 aaaaccatcg caataaccaa tttgaataat aatatctcga agaaacaaag cgagatcacc
     3601 gacctagaaa ctcagataca gaatgacata gaccacatag cagagattat tcggaagacg
     3661 aatcaaatag attcttactc tgtagttgag tctctcttct cagacaaaga tttgtcagaa
     3721 ttttttgtcg acgtggatac ttatgcatcg actaggtcag ctcttaagac agtattagat
     3781 aacttgagag gggtgaaaag tgtaacagag gaggagaagg ccaagctcgc taaagagaga
     3841 gatctccaag cggcagcgaa ggcagagata gaggcaaata agaggaaggt cgaggtagat
     3901 caaaagacac agaagactct gctcacgcag agtcagaacc aggagaaagc ttttgccagt
     3961 cttgtagcag agaagcaagc caaggcagcg gcgatacgca cagcactctt cggcctaaga
     4021 gactctgcgg ccataccttt cggtacggcg ctcgcctatg cgcaggacgc aggggcaaag
     4081 acgggggtga gaccagctct tatccttggc attctccagc aagagtccaa cttgggagcg
     4141 aatgtaggta cttgtgtcat caccgatttg tcttcaggtc agaccaagag tgtgaactcg
     4201 ggcaaggttt ttagtaacgg tatccacccc acaagagact tgccactact ccagtcgctt
     4261 ctaccagctt tgggtcgcga cccacttgtg accaaggtat cgtgcccact ttctataggc
     4321 tacggaggtg ctatgggtcc ggcacagttt attccatcta catggaatat aatgagatct
     4381 cgcatcgcta cagctctaag caaggagacc ccagaccctt ggaacccagc agacgccatt
     4441 atggctatgg cccttttcct caaagatctt ggggcgggct cccagaccta cacagccgag
     4501 aggaatgccg cctgtaggta ttacggaggc ggaacatctt gcaatacacg cacttcgggc
     4561 tatggtaact cggtaatgtc gagggccacc aacatccaga acaacatgat tgacctgttg
     4621 caagatgctt aaaatactgg taaattaaag cccttatgaa gaaagatata catgccaagg
     4681 tttaccgccc agtcctcttt aaggacagcg gtaacgacca cgaatacatc atttcatcta
     4741 cggtagagac caaggatgag ggtaaggcta aggacggtaa gacctatccg ctatttcgta
     4801 ttgaaatatc ttcttcctca caccctttct acactggtca ggagaaggtt cttgactctg
     4861 caggacgtgt agaacgcttc aagcagaagc aggccaaggc tggaacaaca aagaagtaag
     4921 tttgctgtgc acgcaagtat cccgtgctcg ggatcttgct cgcgctcggt ccgtcgacct
     4981 gcgcgaggca cgacaaactt acttctttgt tttttgttaa gcttatgaca atggatattc
     5041 aaaaatacaa agacaaccca aagactctgt atctagcaga gagttttgag cgtctactca
     5101 aagaagagga ggagaccagg gctatggcgg gtgcagacgc tgagatggct aagcttgcag
     5161 aagaagagct aaataatatt gaaatacaaa agaaagagtt gctgactcag atggacgaga
     5221 ttcttaaggc tgaggagaag gaagaagaat tccccaacga aatcatcctc gaagtgagag
     5281 caggtgcagg aggagaggaa gcggctctat tcgccaagga acttgcagat atgtaccgag
     5341 cctatgccaa gcttcagggt tggggtgtac aggttgtgga tgagtcattc tcgagcttaa
     5401 gtggatacaa ggaagcgtct ttcgaagtgc gaggaatgga tgtatacaag aagctgcgtt
     5461 atgagaccgg ggtgcaccgc atacagcgcg tgccagatac tgagaagaca ggcagagttc
     5521 atacttctac ggcctctgtc gcagtgcttc cagttaggaa gaagtcgaag atcgagatca
     5581 agccaacaga cttagagatc gagacgtcac gctctggcgg tgccggcgga cagaatgtga
     5641 acaaggttga gactgcggtc cgaataatcc acaaaccaac aggtattgat gtgcgctcta
     5701 cgagccagag gaaccagctg aagaacaagg agatggccat gtccatactc atggctaagc
     5761 ttgaggcaaa gaagcaggag gaagaggatg ccaagtattc tgcagacagg aagtcacagg
     5821 tgggtacagc tgacaggtca gagaagataa ggacttacaa catcctccaa gaccgcatta
     5881 ccgatcatcg tattaatgag agctggcaca atatccagaa gatattccaa ggttatatgg
     5941 agccaatttt tgaatctttg gaagcagaag caaataaata aacttgactt tgtatacata
     6001 gtgtgataag attgtactaa ctcaggaatg gccaccttgg ggggtctctg gcatgaggcc
     6061 atcgagacat cgggctacac gtgagtagga cacaactgaa agcgcaggac cctcggtggc
     6121 cggcaatgca ggaatgcaac gccgtcgccg ccgaggcaaa cacaatctct cgcaagagag
     6181 tgagggtata gcagttcttc caagacttca ggagaggaaa aacggttcgc ggaataggta
     6241 tgccatagtc taggccagaa gtaatggttc gatgacaaag gtaaccatca gcccgagccc
     6301 ttgaatacta ggggtaaaag cccaaacctc ctggagtcag atttatcctc tctatatgag
     6361 agtgaacaat ataaaccccg acgttacgcg tcggggtgct ttttatatag attgcaagaa
     6421 aagcctatat ttggtaagat tccgaggcac taataaatga caaccaagac ttcaaataac
     6481 agcaatttga tcgatgagct cttccaagca ggggctcatt tcggctttgt aaagtctaga
     6541 cgccatccat ctgctaagcc attcatcttc ggatctaaga acgatgtcga gatctttgac
     6601 ctagagaaga cctcagagga actactcaag gtccttgctt tcgttgagga gaagggtaag
     6661 gagggtgcac aaggactctt cgttggtggt aaatcagagg ctcgtgaggc tataaggaag
     6721 gcaggtaccg agcttgcgca accattcgtc gatggcagat tcattggtgg tactatgacc
     6781 aactggccag agatcaagaa gaggatagct atgctcgaag atcttactac taagagggag
     6841 aagggtgagt taggtcacta taccaagaag gagcgcctac tcatcgacag gaagatcgac
     6901 aagcttactc tctacttcag tggactttct acaatgaagg gtttgccaaa gtttctcatc
     6961 gttgtagatc ctaagaagga acacatcgcg gtcgcagagg ctaagaggat taaaatacca
     7021 gtcattgcta ttgcaggatc tgacaacaat atcttcgacg ttgactttgc tatcccagct
     7081 aacgactcgt ctaaggcgtc tattggcctt ataatggaga agatcgtctt ggcatacaag
     7141 acaggtgcag caagtgttaa ggttgcttaa gttctacaaa tttagataaa aatatggcag
     7201 atataactac cgcagatatt aaagctcttc gtgatgagac agggctctcg attgcagagg
     7261 taaagaaggc attggacgag tcagaggggg acaaggccaa ggctctagag gtcctaaaga
     7321 gcagaagtgc cgaggtcgca gccaagaagg gagaccgtgc tcttggtgcc ggtgtggtta
     7381 atgcatatat ccacaacgga ggtgcttctg gtacgctcct cgagctatct tgtgagactg
     7441 actttgtggc caagaacgag gagtttaaag cacttgcacg tgacattgct atgcatgtag
     7501 cagctatgaa cccagcagat gtggctgagc ttatgccaca accatttatc aagtcaccag
     7561 acatgacaat acaggacatg gtgaacggcg ctacacagaa gtttggtgag aggatcgaag
     7621 tgagacgctt cgttaaactc tcggtttctg agtaattgat ttttcgccta gagcgacatg
     7681 atcttaaaca ttatcttcat cataagtttg ctcggtatcg ttaccttaat catctcaaag
     7741 attttagagg agaagagtaa gggtaagcca ctattcttaa gattagtgtc tttgggtgac
     7801 gagagggtaa ggaatgccac tgtggagttc gcccatatgt actctgactg gagagagagg
     7861 ggacacttct tcctcaacaa tcagttacca ttacacacta agaacctgat gaacaagttc
     7921 tccctcatga tcaaagacaa gttcgaaaag catgtaggag acattagggg ctccaagttc
     7981 ttgaagaaga gcgatggtct ctctgagtat ctaaaaagca tttcagagaa ggannnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccacct tagctcagtt ggtagagcaa
     8161 cacttttgta aagtgaaggt cctcggttca aatccgagag gtggctccaa ggaagtgact
     8221 aataaccata aaatatattt tgagaatctg gactccctca gatttctagc cttttttgtc
     8281 gtgtttgtgt ctcatgcagc attgttcctc ggttttacta gtaacagctc tttgtatcaa
     8341 tcgatcaagt acaacatttt agtaaatggt gatttaggtg tcactttctt ttttgtattg
     8401 tctggatttc ttataacctt cttactactt aaggagaggg aagtaagtgg gggtatttct
     8461 cttaagaaat tttatctaag aaggatatta agaatctggc cggtgtactt tgttgttttg
     8521 cttatgggtt acttcctcat accatatcta gtccagttgt ttggatctcc tagtctccca
     8581 atagatgcat tgcctcccca atctgctttg ccgtggcata ccttttttat aaccaacttt
     8641 gctatggcta gaagtttctt ttctagtgtt ccgacagata ttctttggtc agttggtata
     8701 gaggagcagt tctacctttt gtggcctttg ttggttattc tgacaccaag gagaatgttg
     8761 tcgagagttg tgggtctgat agtctttgca gcttttatat ttagaggcta ctttgcacat
     8821 gaaccagatg tcttagctta ctcaactttc tctgcaatgt cggatttagg gattgggtgt
     8881 ctattggcca tatttgtact tagcgagtat gtttctagac ttagagccat gtttagcaaa
     8941 gtaaccactt ggctgctcta tatattagtt tttgctctaa ctataagtag acacgggtta
     9001 gtaagctttt tttctgatta cgaacatgta tttagtatat ttgtgcctgt tctctatctg
     9061 tttgtctcca ttctttttgc tctcataata ttcgagcaga acgaatcaaa taattcaata
     9121 ttcaaagctg gtaagtttcc cctaatgagt ggtctgggtc gtatatccta cgggctgtac
     9181 gcttatcatc ctgctgtgta tgtatttgtg cttcttataa tgactaagat cggttcggcc
     9241 acgagttatt cgtcaccatt caagtttctg gttgtttcta ttgcagtttt gattagtacg
     9301 attattttct ccaagatttc ctatgcagtg atagagaaat ggttcttaaa gagaaagccg
     9361 tcctccctta catcctcaga tagcttcagt ctatagttat aaacctaatg atttggtggt
     9421 agttgctttc ttctgctgtt ttatctgctc tgctcttaga cgcgatagtg cgggtacact
     9481 ctctaccagc tcatttagag gtctaaagcc gtagtatggg tcaccgttga taacaatggc
     9541 gggcaaggtc tctcctaggc cataaatggt gcggagggtg tgtaaggcct cgagatccat
     9601 gttgtagtcg aaggagtata tacgaaggtc ggggaactcc tcgtgtaagg cggtgagtac
     9661 gaagcccatc ttctgacaat cctcacagtc agtttcattc gcatagaagt agaggataaa
     9721 ggttggattt acgccacact tctcactaat ctttttcatc aataaatagt ctctgatttc
     9781 gaggaggaag tattgcttct tcaaactttg gacatcctga ttgtcgctac cactttgttc
     9841 ttctgcagcg gccaatctct ctgctaactc gccaagttct ttggtaagag gtatttggtt
     9901 gacattcttg caagaggttt cttctaagag tgctgattga gtctcagagg ccaagatatc
     9961 tagagagagc ctattctgaa tgttttggat ctctgctaat ttaccttcgg aaactgagtt
    10021 agatatgaaa agtgctgtag cgaagatggc tacagtcata acaaaagcaa agatgtattt
    10081 ctttgaatca ggcatttatg ggtttacttc cagctaattt tcttctgtgt gatagtacta
    10141 taagtcgcct tagctagcca gaggggtact atgaaggcgt agagcgttag atagtagaag
    10201 acatctctac ccattcttgc cttaccgttc gctaccctta gtgataggta gagaatgacg
    10261 aaggttagag acagtgtggt cacagttatc cagaaagagg tgttcaagtc gaaggcgaac
    10321 cagttgatct tagagaagtt tggcatcccc aggttgaacc caactgcttc gtacttggct
    10381 aaatgctcgg gtattttaga agcgatgctc cataagagat ttcctgcagc atagagggtg
    10441 gtgaagatag agaaggttgc aatcggtaga acgaacatac cgatattccc atatttcttg
    10501 ttaaagaaca tttctttgta atcaattgca ttgttgagga atccgtagat ccagcgcact
    10561 ctctgcttat aaagcttttt taatttatct ggtccaacgg taaacacatg agcactgtga
    10621 gcgttcacta tcttgtagcc gttcttctgc attcttagag ccatctccat gtcctcggta
    10681 tgatgagcgt gtttgtatcc tccaattgtt ttaaaaactt tggtacgaaa gatcgagaat
    10741 ggtccagggg taacgtatag accgtttatg gaagagagta gtcttctaaa aaatattccc
    10801 caactgtact caactctttg catgtactgt agtggtgtct ttgggtcatg aattcttatt
    10861 gatggggtga cggccataac ttcctcttta tcaaagaagg taacaattct ttttagtgct
    10921 gtgggttcta caaatgagtc tgcatcgaga catccaacaa ggtcactgtc gattttctct
    10981 agagcgaagt tgagggcagt gtgtttaccg ccattctctt tgctaaatac ctcgacctga
    11041 ggagtccctc tgaatgtctc tagtaccact gccttagtcc tatctgttga gccgtcatct
    11101 atgaccatga gtcggagctt atccttaggg taatcgagtg agaggataga ctttatggtg
    11161 tcgactactg ttctttcctc gttgtggcag ggtactacta tagtaacggt cgggaatgac
    11221 ttgatatcct tgcctccatg ctctatttcg aagtctatct cgcttttgac ctcaaaatag
    11281 gtaataagca aaaagatctc gaaaaagagc gagacaaaga gcgatatata caatatggcg
    11341 ctagatatgg ccatggatta cttctcacta tagcgtataa tacggcttat gccaaaggca
    11401 ttttgatatg aaaaacctat acaaagtaag cttagaagaa caaaataaat acctccaaat
    11461 tctcgaatcg aggactgatt cagaggcctt aaggatgaag aggtatctcg ccatgaagga
    11521 tctctcaaga actgagggta gcccactaaa ggagatggta gagaatatct tggggcaaga
    11581 aactttcaag gattttgatg ttatagagac cccagaggtt gtctcgacta agatcgggtt
    11641 cgacctcttt gacttcccag ctgaccatcc agcaaggagt aagtctgata cttattatgt
    11701 agatgataat agtttcctcc gcacgcaaac tacagtgatg tggtactact acttgcaaga
    11761 gaaggatgtg caagagagga tggcgcgagg cgaggcagtt ggatgtttct cacacggtaa
    11821 ggtttatcgt aaggacgaga tagacagaca tcatatgaat gtctttcatc agcttgatgg
    11881 ttggtatcta gctccaaaag agaaggagac tataggagaa gaagatttga aaaatgtttt
    11941 gaactctatt gccaaggctg tgtatggaga tagtgctatt actcgcttca atccagacac
    12001 cttcccatat acacacccat cactcgagat ggagatagat aaggatggta aatgggttga
    12061 gcttctagga agtggggtgg tgaagggaag tgtgttagag aagtttggta tcgatagtac
    12121 taagtggact ggttgggcct ttggcttcgg actcgagcgc ttggccatag tgtctatgga
    12181 gctgccagac attagactcc tttggtcaga cgacgagaga gttgctaagc agttggtgtt
    12241 aggaaataaa tacaaagaag tgagtaagtt cccaccagtg gtgcgcgaca tttcttttgt
    12301 tgtggataaa tcatctttcg tattaaacga ttactttgat gctatccgag atatcggcgg
    12361 tgagatggtc gaagaagtgg cacttataga caagtatgag aatgctgcca aattcggtga
    12421 gggtaaaatg agttatacct ggcgagtgac ataccgagat ttaaacaaga ctcttacgaa
    12481 tgaggaggta aatgcattgc acgccaaggt cgaagagatg acggttaagg attataaggg
    12541 agtaataaga gtgtcatgag ttacttagtt ggactgtcca aagatgagat aagtgaggac
    12601 acgcttaaca agtctggtct caagtggaag aaggtaaatc ctagagaaac tattttatct
    12661 atagctccat ctctactagg cgtaccatat aagttcggtg ctagtgtgtc taaggatagt
    12721 ccaaatgcct tcgactgctc ctctcttact gcttacttgt acactcaggg tggtgtggcg
    12781 atacctaggg tttgtgatga tcagtataaa gccggcgatg aagttcctga gacagaagct
    12841 ctgccggggg atttggtttt ttataaaggt aatagaactg acatatcagc agataaggtt
    12901 ggtcatgttg gagtatatat gggtgagggg gatatcatac acgctggtgg agttagtatt
    12961 gggtatggca aagtagtgag agagaagatt acagaatcaa aatattatcc aagcggattt
    13021 ttaggttata gaagattatt acagagtggt gatgataggt ttgtagttga gataccagaa
    13081 gataggccag aactaagaga tagaaacatt ttaataaagg aattgcagag tttgtgatac
    13141 tatttcagaa atatgaagac
//
LOCUS       DAORIT010000018         9429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_1261_length_9429_cov_53.059526, whole genome shotgun sequence.
ACCESSION   DAORIT010000018 DAORIT010000000
VERSION     DAORIT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1261_length_9429_cov_53.059526"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLD99_02210"
     CDS             complement(<1..437)
                     /locus_tag="PLD99_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25748.1"
                     /translation="MAKNGSSTLEWEAPEHEHKERSSDWFWVTGIITLGIILVSIIVG
                     NIIFGILVLVSAVSLVIFINKKPENLKLAVTEDGVQREGVFYPFETLESFWIDLEHPH
                     NKIIIKSKKMLMPLIIVPLADGANVEEIRNMMVKNIEEEYHSLP"
     gene            complement(465..549)
                     /locus_tag="PLD99_02215"
     tRNA            complement(465..549)
                     /locus_tag="PLD99_02215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(513..515),aa:Ser,seq:tga)
     gene            complement(574..786)
                     /locus_tag="PLD99_02220"
     CDS             complement(574..786)
                     /locus_tag="PLD99_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HRH25749.1"
                     /translation="MEAWIIAHPIISALALIWIMAWKGLALWKSAELQQKPWFIAILI
                     VNTFGLLEIFYLFVIARKYTVEVVEN"
     gene            812..1192
                     /gene="ruvX"
                     /locus_tag="PLD99_02225"
     CDS             812..1192
                     /gene="ruvX"
                     /locus_tag="PLD99_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRH25750.1"
                     /translation="MRLMAIDYGGKNVGIASTDEAGNFAIPRMVLPNDKDLLNAVSRL
                     SEDWQIGRIIVGESLNYKNEPNPIDEEAKAFAESLRMLGKTVEFHPELMTSLEAQHIQ
                     GDIPMLDASAAALILKSYIDTNRA"
     gene            1189..1515
                     /locus_tag="PLD99_02230"
     CDS             1189..1515
                     /locus_tag="PLD99_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25751.1"
                     /translation="MINIDEFKKTEITIGEIKKVEDVEGSDKLLKLTVDFGEEVPRTV
                     LSGIKKYFANPQTLIGVKSPFVTNLEPRPLMGMVSEAMILAVSTEDKFSLLSVGEEIP
                     IGTKVK"
     gene            1528..2502
                     /gene="pilM"
                     /locus_tag="PLD99_02235"
     CDS             1528..2502
                     /gene="pilM"
                     /locus_tag="PLD99_02235"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRH25752.1"
                     /translation="MKLFDIFPTPRFLTLTDAGVSISERTVRYISFRPSGSGDLHIEL
                     SGEIALPEGVVSQGEVKDKEKLVEVLSTLSRDKGIKFVKATLPEERAYLFTTKVDRVP
                     YKDLRDAVAFLIEENAPVSLSKSLFSFEILDNPEDFKVAVSVLPTDVAQTYIETFEAA
                     GLTPVSFDIESQAISRSLVKRGDERAHLIIYLGHNRVGFYLVNDGVVQFSTTMLIDVA
                     DSENPETIKALKLEIRKFFSFWSSKVDAGSVPNQKVERIIIGGPWAKDEKLVSSIMSD
                     VETPYSLGNVWINVSDIEKRLPDMSFEESLAYAAAVGAALRGGREEYV"
     gene            2495..3040
                     /locus_tag="PLD99_02240"
     CDS             2495..3040
                     /locus_tag="PLD99_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25753.1"
                     /translation="MYKLLTPEETNKVKKEYSLRRLSVSLALLSAVAMFMIITLLPAL
                     IFTIKKKESALFTLSTVNKPLRLTNKQSLDAWVERTKLELTALTPDGWPDTPYIYFKK
                     IIDKKPNSITIGSLSFSRAANGNKNTRVVGTASNRKELLAFQSALNESGDWTQVDLPL
                     STIAREADIPFEMNLVPQKIK"
     gene            3037..3558
                     /locus_tag="PLD99_02245"
     CDS             3037..3558
                     /locus_tag="PLD99_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25754.1"
                     /translation="MNSVTRTIIVGLIFLIMLGGVGYELYYIKSADSAVRNAISKADE
                     QIGSDSRARSVVYLQNNSVEEIKVLDKAILTKDELVTFVESLETMGRNMGLVVAISSI
                     TKEGNKSSTSTPEVVRITVDATGPWGESLHFINLLENLPYKIGIDKVDFNSDEKGWRS
                     NTTIKITTYPEKS"
     gene            3555..3863
                     /locus_tag="PLD99_02250"
     CDS             3555..3863
                     /locus_tag="PLD99_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25755.1"
                     /translation="MKIFDKDGYKNKGRFLGWGSGPKEDWMILFGVFCVSLLIVAAYS
                     GWVFYDSMRGEFASETNVIAESPIDIKVIKSTAAQYTTKSASFETLLKTKETTPDPSK
                     "
     gene            complement(3866..3940)
                     /locus_tag="PLD99_02255"
     tRNA            complement(3866..3940)
                     /locus_tag="PLD99_02255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3905..3907),aa:Arg,seq:tct)
     gene            complement(3995..4067)
                     /locus_tag="PLD99_02260"
     tRNA            complement(3995..4067)
                     /locus_tag="PLD99_02260"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4032..4034),aa:Val,seq:tac)
     gene            4112..4831
                     /locus_tag="PLD99_02265"
     CDS             4112..4831
                     /locus_tag="PLD99_02265"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRH25756.1"
                     /translation="MATDPEILYEDKDILVINKPAGLVVHSDGKTVEPTVSEWLVSKY
                     PEAKEVGEDMGEVERPGIVHRIDRDTSGVLVLAKTKEGHANLKEQFKERLIDKVYETF
                     VYGKLKEEHGSIKLPIGKSNSDFRKWSAERGAKGVKRDALTYFRVLGRGDGLTYIEAK
                     PKTGRTHQIRVHFKALHHPVVCDKLYAAQKEPMLGFERLALHARSLTFKDLSGQSHTV
                     VAPYPEDFKRAIDTLGKPVLD"
     gene            4840..5244
                     /gene="rplS"
                     /locus_tag="PLD99_02270"
     CDS             4840..5244
                     /gene="rplS"
                     /locus_tag="PLD99_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRH25757.1"
                     /translation="MKKVLNRVITPVKVEDRANIDLRSGDTVRVWQKIQEKGKTRLQA
                     FEGLVLARKHGTEAGATFTVRRIASGVGVEKIFPLYSPMIDKIEIVKRAKVRRAKLYH
                     IRDKAAKEVRRQMRNERFAAPTSVVEETVEEK"
     gene            5257..5342
                     /locus_tag="PLD99_02275"
     tRNA            5257..5342
                     /locus_tag="PLD99_02275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5291..5293,aa:Leu,seq:gag)
     gene            5454..6194
                     /locus_tag="PLD99_02280"
     CDS             5454..6194
                     /locus_tag="PLD99_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25758.1"
                     /translation="MAKKYIYSNHEEFMDKSNSLRDRRRIESTTWAMIDAVRSAGYRP
                     AGKRSREVNRILADINSEFLDRVTNLLKNLRFDEKVQQVEGQLDLKLVSSLESLVSGP
                     RRRRLDSVRSLKKSLSHRRETMPRDRAMSILDIHEIAVYWAATLSVRGWRGVVDYRFM
                     EMLKNEARFILQEYGYTLALGKSINCPFITAWQDHGFWRHICVLDGVSSAEINAQKEL
                     LRRTGCTKGCPGYFAEPRCMQTPLDMSA"
     gene            6253..6323
                     /locus_tag="PLD99_02285"
     tRNA            6253..6323
                     /locus_tag="PLD99_02285"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6285..6287,aa:His,seq:gtg)
     gene            6590..7168
                     /locus_tag="PLD99_02290"
     CDS             6590..7168
                     /locus_tag="PLD99_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25759.1"
                     /translation="MPAYIRGHAFDRVSERLQMSPQEVADLLDQERYVLIGIEDGSSV
                     AHKLIYSVLDNSWFVVIQDDVCGHVITILPAAFEKRMEVSADAIRQARLLAEGELPLK
                     SAPSGFTGPSFWFNAVIKDRRGITRTISLKPVPLVGEATIDAVLEERPMIEYIKKEIS
                     GKILFMEKVVSVDIRIGRKGKRVSIDLRRLYR"
     gene            7269..7661
                     /locus_tag="PLD99_02295"
     CDS             7269..7661
                     /locus_tag="PLD99_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25760.1"
                     /translation="MPYPQVSQELESGTTLNARARKKGKSNETSQKKREMMNGHIGHF
                     SELALARLNRRPWEPSNKRGKSAFAMQKEICATYNNLPSTDFGRLITIAGRKDGPIDV
                     RMFLSKRLDNPGYTLQEYIDSTPSLRGH"
     gene            7713..8411
                     /locus_tag="PLD99_02300"
     CDS             7713..8411
                     /locus_tag="PLD99_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRH25761.1"
                     /translation="MIGLYDDRMNLTRAIKNIFFLLVAVFSGLTLVVITAAVSAGGGE
                     LSPFNSAIETAISLLRNPPLSSLMLLITNIGSPFVLTSLSIILGVILLSKNDTYDTLL
                     YVFSMALSIVTFVIMKNALSVPRPVDTMLMELTGWSFPSGHATVATAFFFVTGYSFFN
                     WPKSWTLKIFLATLCVIGALLVSFSRVYLGAHFAIDVLAGMALGLLTVSFSILVFNIF
                     LSEREWWRRRVRSL"
     gene            8424..9197
                     /locus_tag="PLD99_02305"
     CDS             8424..9197
                     /locus_tag="PLD99_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRH25762.1"
                     /translation="MQQFFEQKLGRAVLILLVVFLGVQTLSIFKNLGEPSAAYNTIVV
                     TGQGESFAAPDVASFSFSVSADAKTVGSAQDTVTEKTNAILEALKGMGIEEKDIRTTD
                     YSVFPKYTYESAVCAYGGYCPPTKQVPDGYTVTHTILIKVRATADAGQALSLAGANGA
                     TNISSLTFTIDDPDMVMNEARDEAVAEAKAKAKALAKSLGVRLGRVVDFTDSTYGGVY
                     PTMYRGEAMGMGGADSKVIDIPTGENKVSSNVTITYEIR"
     gene            9266..>9429
                     /gene="secE"
                     /locus_tag="PLD99_02310"
     CDS             9266..>9429
                     /gene="secE"
                     /locus_tag="PLD99_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRH25763.1"
                     /translation="MKRFIQYIKDTRAELVHVKWPTRREAMSFTLLVILVSILTSVLL
                     GFFDYLLSLV"
BASE COUNT         2581 a         1987 c         2331 g         2530 t
ORIGIN      
        1 gggagtgagt gatactcttc ttctatattt tttaccatca tatttctgat ttcttcgaca
       61 tttgctccgt cggctaaggg gacgatgatt agtggcatca acatcttttt agacttgata
      121 atgatcttat tgtgagggtg ctcgaggtcg atccagaagc tctcaagcgt ttcaaagggg
      181 taaaacaccc cctctctctg cacgccatcc tctgttacag cgagcttcaa gttttcaggc
      241 ttcttattga tgaatattac aagagagact gcgcttacga ggactaggat gccaaagatg
      301 atattgccaa cgatgattga gacgaggatg ataccgagag taatgatacc tgtaacccaa
      361 aaccagtcag agcttctttc tttgtgctca tgctctggtg cctcccactc gagtgtcgaa
      421 gatccgttct tagccatgcg ctaattttat cacacaaatt tttgcggagg aggtgagatt
      481 cgaactcacg gtcccttgcg agactcttgt tttcaagaca agtgcgatag accactctgc
      541 cactcctccg tgggctctat aaaaccataa aactcagttt tctaccacct ctacggtata
      601 tttgcgagcg atgacgaaga ggtagaagat ctcgagtagg ccgaaggtgt ttactatgag
      661 tattgcgatg aaccatggct tttgctgtag ctcagcagac ttccacaagg ccaatccctt
      721 ccatgccatg atccaaatga gggcgagtgc tgagattatg gggtgtgcga tgatccaagc
      781 ttccatcagt taattatact ataatgcgtt catgagactg atggcaattg attatggggg
      841 gaagaatgtt ggtatcgctt caacagatga ggcagggaac tttgctatcc ctcgtatggt
      901 cttgccgaac gataaggatc ttctaaacgc agtctctagg ctatcggaag attggcagat
      961 agggaggatt attgttggcg agtctttaaa ttataaaaac gaaccaaatc cgattgatga
     1021 agaggccaaa gcctttgccg aaagtctaag aatgctcggc aagacggtcg agtttcaccc
     1081 agagcttatg acttccttag aggcacagca tatccaagga gacataccta tgctcgatgc
     1141 ctcggcagca gcattgatac ttaaaagtta tatagacaca aacagagcat gatcaatatc
     1201 gatgaattca agaagacaga gataactata ggcgagataa agaaagttga ggatgtagag
     1261 gggagtgata agctcttgaa gctcactgtg gacttcggtg aggaggtgcc tcgtacagta
     1321 ctctcaggta tcaagaagta tttcgccaat ccacaaacac tcattggtgt taagagccca
     1381 ttcgttacta acttggaacc tcgtccgctc atgggtatgg tgtcggaagc gatgattttg
     1441 gcagtttcta ctgaggacaa attctctctt ctctctgttg gggaggaaat accaatcggt
     1501 acaaaagtga aatgatatac tagtgggatg aagttgtttg atattttccc tacacctcgc
     1561 tttctcaccc tcactgatgc gggggtctct atctctgaga ggacagttag atacatttct
     1621 ttcagaccgt caggaagtgg agatttgcat atagaattat caggagaaat tgctttgcca
     1681 gagggagtag tctcacaggg tgaggttaaa gataaggaga agttggtcga agtactctcg
     1741 acgctctcta gggacaaggg tattaagttt gttaaggcca ctcttcctga ggagagagcg
     1801 tatctcttta ctaccaaggt tgatcgagtg ccatacaagg atcttcgaga tgcagtcgct
     1861 ttccttatcg aggagaacgc tccagtatcc ctttccaaat ctctcttctc gtttgaaata
     1921 ctcgataacc cagaggattt taaagtcgct gtatcggtcc tgccgactga tgtagcacaa
     1981 acttatatag agactttcga agcagcggga cttactcctg tatctttcga cattgagtct
     2041 caagccatat ctagatcctt agtgaagcgt ggtgacgaga gagcgcatct tataatttat
     2101 cttggacaca acagagttgg cttctatttg gttaatgatg gtgtggtcca attcagcacc
     2161 actatgttga tagatgtcgc tgactcagag aacccagaga ctatcaaagc gttgaaactt
     2221 gagatcagaa agtttttttc tttctggagt agcaaggtag atgcgggctc ggtcccaaac
     2281 cagaaggttg aacgcataat cattggtgga ccatgggcca aggatgaaaa acttgtttca
     2341 agtatcatga gcgatgtaga gacgccatat tcactgggta atgtgtggat caatgtttcg
     2401 gacattgaga agcgtttgcc cgatatgtcc tttgaagagt ctctggccta cgcagcagca
     2461 gtcggtgccg cattaagagg tggtagagaa gaatatgtat aaactactaa caccagaaga
     2521 aactaataaa gttaagaaag agtattctct cagacgactt tctgtatctt tggcactact
     2581 ttcggcagtg gcgatgttta tgatcataac ccttcttcct gcactgatct tcactatcaa
     2641 gaagaaagag tcggctctct ttaccttaag cacggtcaac aaaccactca gactaacgaa
     2701 caaacagagt ttggacgctt gggtcgagag gaccaagctc gagctaacag cactcactcc
     2761 cgatggttgg cccgacacac cttatatcta tttcaaaaaa ataattgata agaagccaaa
     2821 ttcgataact ataggttctt tgagtttctc tagagcagcc aatggcaaca agaataccag
     2881 ggtcgtgggc accgcctcaa ataggaagga gctcttggcc ttccagtcgg cgcttaatga
     2941 gtctggagat tggactcagg tagatctgcc gcttagcacc atcgcaagag aagctgacat
     3001 accgtttgag atgaacctag tcccacaaaa aataaaatga actcagtaac aagaacaatt
     3061 attgttggac tcatcttcct catcatgctc ggtggggtgg gctacgagct ttactacatt
     3121 aaaagtgctg acagtgcggt gaggaacgct ataagcaagg cagatgaaca aataggtagc
     3181 gatagtagag ctaggagtgt tgtttatctt caaaacaatt ctgtcgaaga aattaaggtg
     3241 ttagataagg ccatactaac caaagacgag ttagtaacct ttgtagaaag tttagagacc
     3301 atgggcagaa acatgggact ggttgtcgct atctcttcta tcaccaagga ggggaacaag
     3361 agctctacct ctacccctga agttgtaaga attactgttg atgctaccgg cccttgggga
     3421 gagagtctcc acttcataaa tcttttagaa aacctacctt ataagattgg aattgataag
     3481 gtcgacttta acagtgatga aaaaggttgg agatctaaca ctacaatcaa aataacaact
     3541 tatcctgaaa aatcatgaaa atttttgata aagatgggta caagaataaa gggagatttc
     3601 tcggttgggg tagtggtccc aaggaagact ggatgatttt gtttggagta ttctgcgtgt
     3661 ctctacttat tgttgctgct tattctggat gggtgttcta cgactcgatg agaggagagt
     3721 ttgcctcaga gaccaatgtt atagctgagt caccaataga tataaaggtt atcaaaagta
     3781 ctgcagctca gtatacaacc aagtctgcct cgtttgagac acttcttaaa accaaggaaa
     3841 ctaccccaga tccttctaaa taatttggtg cctccagcag gaatcgaacc cacatcaact
     3901 ccttagaaga gagctgctct atccattgag ctatggaggc gagaacacaa gttagcatat
     3961 acgatttttt caaacaaaac cagaaagata agtgtgggcg atacaggact cgaacctgtg
     4021 accttctctg tgtaaaagag ttgctctacc aactgagcta atcgcccgct tggacgatac
     4081 taacatatgt agtagtctct tggtaagtac catggccact gatccagaaa tactctatga
     4141 ggacaaagat atcttggtta taaacaaacc ggccggtctt gtggtgcata gcgatggtaa
     4201 aactgttgag ccaactgtga gtgagtggct tgtatcgaag tatccagagg ctaaggaggt
     4261 gggggaggac atgggagaag tagaaagacc tggtatcgtg caccgtattg accgcgacac
     4321 ttccggagtg cttgttttgg cgaagaccaa ggagggtcac gccaacttga aggagcaatt
     4381 taaggagagg ttgatagaca aggtctacga aacatttgtg tacggcaagt tgaaggagga
     4441 acacggctcg atcaagcttc cgattggcaa gagtaactcg gactttagaa agtggtctgc
     4501 cgagagagga gctaaagggg tcaagcgaga cgccttaaca tactttagag ttctggggcg
     4561 tggagacggt ttgacatata tagaggcgaa gccgaagacc ggcaggacgc accagatccg
     4621 tgtccacttc aaagcacttc atcatccagt ggtttgtgac aagctatacg ctgcacagaa
     4681 ggagcctatg cttggcttcg agcgcttggc actacatgcc agaagtctta ccttcaagga
     4741 cctttctggc cagtctcata ccgtcgtagc gccatatccc gaggacttta agcgcgctat
     4801 agacactttg ggcaaacctg tgttagattg agtccctcaa tgaaaaaggt attaaacagg
     4861 gttattaccc cagttaaggt agaggacaga gccaatatcg acctcaggtc gggagacact
     4921 gtacgcgtct ggcagaagat acaggagaaa ggcaagaccc gtcttcaggc tttcgaaggt
     4981 ctagtccttg ctcgtaagca cggcacagag gcgggagcca ccttcactgt tcgtcgtatt
     5041 gcctcaggtg taggagtaga gaagatcttc cccctatact ctccaatgat cgataagatc
     5101 gaaatagtta agcgtgccaa ggttcgtcgt gccaagcttt atcacatccg cgacaaggct
     5161 gctaaggaag tcagacgaca aatgagaaac gagcgcttcg cagctccaac ttctgttgtt
     5221 gaagaaactg tagaagagaa gtaagattgg cgtattgcgc gggtggcgaa attggtagac
     5281 gcactacctt gaggtggtag cgcccattgg gtgtggaggt tcaaatcctc tcccgcgcac
     5341 caaaacaaaa tcagtaacac aagttactga tttttgtttt acctatagac acttcctcca
     5401 agatatttct ctataggctg tgcacatata tttttattca catagagtta tgattggcaa
     5461 agaagtacat ttactcgaat catgaggaat ttatggacaa gagtaactct cttagagatc
     5521 gtagacgaat cgaatctacg acttgggcca tgattgatgc ggtgagaagt gctggctatc
     5581 gccctgcagg gaagaggagt cgagaagtta atagaattct tgctgatatc aattctgaat
     5641 tcttggacag agtgaccaac ttactcaaga atcttcggtt cgatgagaag gttcagcaag
     5701 ttgaaggaca gcttgatctc aagctcgtat cgagtctgga atcattggtc tctggtccta
     5761 gacggcgcag gttggattct gtcagatctc tcaagaagtc tcttagtcat aggcgcgaaa
     5821 ccatgcctcg cgatagggct atgagtattc ttgatattca tgaaattgcg gtttactggg
     5881 cagcaaccct atcagtaagg ggttggcgag gagtcgttga ttacagattc atggagatgc
     5941 ttaagaatga ggcccgcttt atcttacaag agtacgggta cacccttgct cttggaaaga
     6001 gtatcaattg cccattcatc accgcgtggc aggatcatgg gttctggaga catatctgcg
     6061 ttcttgatgg tgtatcaagt gccgaaatca atgctcagaa agagctgttg agacgcactg
     6121 ggtgcaccaa gggttgccct ggatactttg ctgagccgag atgcatgcaa acacctctgg
     6181 atatgagtgc ctagtagacc gctgtaatag gcggtttttt tctatatttg tggtataaag
     6241 atggagttaa tggtggctat ggtgtagcgg tagcacgcga gcttgtggag ctctctgtca
     6301 gggttcgact ccctgtagtc acccaagatc aaaaacactc tcttgcgaga gttgtttttg
     6361 tatctgtgtt ttaggagtcg aacaaggaga gggggtcggg gagacgcgga tcgtctcccc
     6421 gtggagggag gattgggaaa accgtgggtt tcccagtagt gagacatccc tgtagtcacc
     6481 caaatattga ctttttgttt ttgggtgtta tttttaaatt tgaatgagtt gattcaagtt
     6541 agcacaaggc taacttgata aacttttgaa ctttttatgg aggatttaga tgcctgcata
     6601 catcagggga cacgcttttg atcgtgtatc tgagagactt cagatgagtc cgcaagaagt
     6661 ggcagatctg ttggatcagg agaggtatgt tttgattggt atagaagacg ggtcatctgt
     6721 cgctcacaag ctcatttaca gtgttctcga taacagctgg ttcgttgtta tccaagatga
     6781 tgtgtgtggg catgtgatca ccattctccc cgcagcgttt gagaaaagaa tggaggtttc
     6841 ggcagatgcc attcgtcagg caagacttct ggctgagggt gaacttccac tgaagtctgc
     6901 gccaagtgga tttactggac cgtccttctg gttcaatgca gtgattaagg accgaagggg
     6961 aatcacgcgg acgatcagtc tcaagcctgt tccgctagta ggagaagcaa ccatcgatgc
     7021 tgttttggaa gaaagaccaa tgattgaata tatcaaaaaa gagatcagtg ggaagatctt
     7081 gtttatggaa aaggtagttt cagttgatat aagaattggc aggaagggga aacgagtttc
     7141 tattgatctg cgccgcctct ataggtgagc gcatttttgt ttgacaatct atatatttct
     7201 gatactttga aagagggcaa atccgccctt ttttgttcat ttcacatttt gtattgaggt
     7261 agcgtaacat gccctacccg caagtctcgc aggagctgga atccgggacc actctcaacg
     7321 ctcgcgcccg caagaagggt aagagcaacg agacttcgca gaagaagagg gagatgatga
     7381 atggccatat tggccatttc tctgaactcg ctcttgcacg actcaaccgg cgaccgtggg
     7441 agccaagtaa caagaggggt aagtcggctt tcgccatgca gaaggagatc tgcgcgacct
     7501 acaacaatct gccgagcact gacttcggca gactcatcac catcgcaggt cgcaaggatg
     7561 gaccaatcga cgtcaggatg ttcctctcga agaggctcga caatccgggg tacactcttc
     7621 aggagtacat cgacagcacg ccgtcgcttc gcggccacta agccacgatc aacgagtgcc
     7681 ccgaccaaat ggtcggggcc ttttcttttt ttatgatagg cttatatgac gaccgtatga
     7741 acctcacaag ggctatcaaa aacattttct tccttcttgt ggctgtattc tccggcctta
     7801 ccctggttgt cattaccgct gcagtatccg caggtggtgg agagctctcg cccttcaact
     7861 cagctattga gactgccata tctcttctcc gcaacccacc tcttagtagt ttgatgcttc
     7921 ttattaccaa tataggttct ccattcgtcc taacatccct atcgatcatt ttgggtgtta
     7981 tcctcctttc gaagaatgat acttacgata ctctcttgta tgtattctct atggctctct
     8041 caatagtcac ttttgtgatt atgaagaatg ctttgagtgt ccccaggcca gtagacacca
     8101 tgctcatgga gcttactgga tggagcttcc cctcgggtca tgccactgtg gcaactgcct
     8161 tcttctttgt tacggggtac tccttcttca actggccgaa gagttggaca ctcaaaatat
     8221 ttttagccac actatgcgtc attggggcac tacttgtatc attctctaga gtttatctgg
     8281 gagctcactt tgctatagat gtattggctg gtatggcact tgggctactg acggttagct
     8341 tctcaatact cgttttcaac atctttttgt cagagagaga gtggtggagg aggcgagtaa
     8401 ggagcctgtg atatactcct agcatgcaac aatttttcga acaaaagctc ggtagagcag
     8461 tgcttatatt attagtagtg tttctcggtg ttcaaacgct aagtatattt aagaacctag
     8521 gagagccaag tgctgcttac aatactattg tggttactgg tcagggtgag tccttcgctg
     8581 cacctgatgt agcgagcttc tccttctcag tttcagcaga tgctaagact gtaggtagcg
     8641 cgcaagacac tgtgacagag aagaccaacg ctattctcga ggctcttaag ggtatgggta
     8701 tagaggagaa ggatattagg actaccgact actcggtctt ccctaagtac acttacgagt
     8761 ctgctgtttg tgcgtatggt ggttattgcc ctccaacaaa gcaggttcca gatggataca
     8821 cagtaactca cacaatctta atcaaagtta gagctactgc cgacgctggc caagcactct
     8881 cactcgcggg tgctaatggt gcaaccaaca tctcatcact aacatttact atcgacgacc
     8941 cagacatggt gatgaacgag gcaagagacg aggctgtagc agaggccaag gccaaggcca
     9001 aggcactcgc taagtcctta ggggttagac ttggtcgtgt tgtcgacttc actgacagta
     9061 cttatggtgg tgtgtatcca accatgtaca gaggtgaggc tatgggtatg ggaggagccg
     9121 actctaaggt cattgatatc ccaacagggg agaacaaggt gagctcaaac gtcactatta
     9181 cctacgaaat aaggtaattg acctagaggt tcgagtgtag tattatctaa aaagcgccga
     9241 ggggcgtttt ttagtaaaaa aagaaatgaa gagattcatc caatacatta aagacacaag
     9301 agctgagctg gttcatgtga agtggccaac caggagagag gcaatgtcct tcaccctgct
     9361 tgttatactc gtgtctattc ttacctcagt tctacttggg ttcttcgact acctactctc
     9421 actcgttat
//
LOCUS       DAORIT010000019         9040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_1341_length_9040_cov_52.470117, whole genome shotgun sequence.
ACCESSION   DAORIT010000019 DAORIT010000000
VERSION     DAORIT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1341_length_9040_cov_52.470117"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..917)
                     /locus_tag="PLD99_02315"
     CDS             complement(306..917)
                     /locus_tag="PLD99_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25764.1"
                     /translation="MKFFRLFVLFLLIASPLEAQDWEFGLYHQRVKKADSLGIDLSSI
                     NNYIDTWAKVHGRTGVWGFAYSETGYSSLVGGLYYDLTDWFELGLGAGLESVSGEEKK
                     SERYAAFLLLFSGRTCTLEVYYERGRVVANPWHQADLLCRPVDGIALGIFSQRFVGTG
                     PRLVLTLTDKIPLQLWLSPFMYDNETKTSSKALGVQLVFRDKK"
     gene            complement(1039..1686)
                     /locus_tag="PLD99_02320"
     CDS             complement(1039..1686)
                     /locus_tag="PLD99_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25765.1"
                     /translation="MRWFVRFLSFILLTSMLGTSAMAQQNPSAMSLQSITVGSGRDAI
                     ASGIVGTAIFGSNDGKRYGDFTIQPQQAWLTFARNYGTKTKATIGSTIGYFQGAPWVG
                     PYVYVNQPITRSISVSGLYWPAVFVWEPSDWKNDGVKNPESFAFGNFGMMSIGTDRVK
                     LTYAMLDFLDDPFNRLPGISISAPYAERMTFTANSTWNTVAEEWMFYMGMTWSPK"
     gene            complement(1824..2300)
                     /locus_tag="PLD99_02325"
     CDS             complement(1824..2300)
                     /locus_tag="PLD99_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25766.1"
                     /translation="MYVTGDGGSFPQMDISGSIKLTPKTGMFIWSLVSQNWGESITGL
                     TYSPKNWIEFGFGAGVEKHDNPLRAMGRVWIGNSKGSLFTAVEQGGSGHWYLVDGNIP
                     LPQTNIGVGFRLQRFIGLGPRLQVTTKAVTLIVVPIAWDIESRGASNSLFVLRITP"
     gene            complement(2480..3832)
                     /locus_tag="PLD99_02330"
     CDS             complement(2480..3832)
                     /locus_tag="PLD99_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRH25767.1"
                     /translation="MASIDEIRETRIKKLEALIEKGINPYPTTSHFDKTLADAAKDFV
                     EGERVQLVGRVMAIRGQGKVIFIDIDDGTGKFQALLKKGDPTKDEDFELFSEYFDIGD
                     FIEIKGELFVTKQNEKTLLVEGLRMLAKSLRPLPEKWAGLTDIEERFRKRYLDILSNP
                     EVKARFLTRSKIVSLIREFYLKHGYVEVDLPNLQPLAGGATAAPFTTHHNALDMDFYL
                     PIAQELYLKELLAGGMPKVFEIGKRFRNEGIDSTHNPEFTMLESNSAYEDAVSQREFI
                     QELFRYVVKGIFGKLQFEYEGQIIDLEPDFKVMKFPDTTDEEYKKNLRPTLIQPTFLI
                     DYPVAFNPFAKRKEEDPTLIDRFQLVICGAELVNAFSELNNPIDQKERYLEEDKKGKG
                     GNKDISPSDMEYLEAMEYGMPPNGGIGIGIDRLVMLLTGVSNIKEVILFPTLKPKNRD
                     "
     gene            complement(3837..4289)
                     /gene="greA"
                     /locus_tag="PLD99_02335"
     CDS             complement(3837..4289)
                     /gene="greA"
                     /locus_tag="PLD99_02335"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRH25768.1"
                     /translation="MEYLTQAKYNEFKAELDVLKTEKRTEIAKQLEYAKSLGDLSENA
                     EYHEARNAQALVEDRIIYLEHLLRSATIVSNNNTDSVSVGGVVTLKKDDGTKKIFTIV
                     GSEESDAASGKISVKSPIGVAALGKKKGESFSVVTPNGTMTYKVIDIE"
     gene            complement(4345..6054)
                     /locus_tag="PLD99_02340"
     CDS             complement(4345..6054)
                     /locus_tag="PLD99_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRH25769.1"
                     /translation="MHNFLRRIRKSGRGRNKEINPEDIFIDSANLPGLNRDRFEGRME
                     SPIGENTFLLFKVALVLVFLLLSTKLWALQVSDGSLYKQVSEENRLSRYIIFSDRGVI
                     FDRNSVPLAENVLYATSSDFALRKYPDIKGMSALVGYVKHPLKDSSGRYYDETYRGEL
                     GVEKTYNEILKGENGSKLVETDALGDITSESVVLLPKKGGDIFLSVDSRLNEKMYKTI
                     ESLANEKGFTGGAGVIMDVNTGEILALTSYPEYDQNLVAEGANKPEINKLLNDKRTPF
                     LNRVVSGLYTPGSIVKPIVALGALNEGVIDPYKEILSTGALTLPNPYNPDKPSIFKDW
                     KAHGLVDMRKAIAMSSDVYFYEVGGGFGTQKGLGITKLDEYFSLFGLNEMTGIDLPSE
                     SLGYIATPSWKEINFPDDPVWRVGNTYHTSIGQYGTQITPISAVRFVAAIANGGKMLK
                     PSVLLGGQTTEAKVTRTIDLKKEYFDVVREGMRQTVTAGTASGLSKPEVKVAGKTGTA
                     ELGLRKEFVNAWVVGFFPYESPRYAFVVIMEKGPVKNTVGGVYVMRQVLDWMVENTPE
                     YLN"
     gene            complement(6047..6301)
                     /locus_tag="PLD99_02345"
     CDS             complement(6047..6301)
                     /locus_tag="PLD99_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25770.1"
                     /translation="MRTLTTIMLGLSIVFAPAWVYLSVLFIAIFLHPYYLEGIVAGLL
                     IDTLYGARAGAPLFGYIFGFSATVAIIFVTPLRKYLRFHA"
     gene            complement(6303..7085)
                     /gene="mreC"
                     /locus_tag="PLD99_02350"
     CDS             complement(6303..7085)
                     /gene="mreC"
                     /locus_tag="PLD99_02350"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRH25771.1"
                     /translation="MKTNFHKSYRRINRDKERRLTYIFGAFIFMALVVTLFKSPILGL
                     VSPLWQGQNFLARSFKELSLMIKDKNALVEENILLKEKLVLLDSLESSVRNYEDTQTE
                     MLELFGRNGVGDFIAATVIARPPKTLYDILVLDAGEVNGIRDGARVSDPVLGELGTVV
                     EVSQFNSKVSLYSTSGRETSAFLDRDDEPVTLIGRGGGTFFFTVPKDVLVMPGDRILL
                     GSIDAPLVAVVEASFIEPTDSLKKVLARSVVNMHKIRFVEVR"
     gene            complement(7057..8118)
                     /locus_tag="PLD99_02355"
     CDS             complement(7057..8118)
                     /locus_tag="PLD99_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRH25772.1"
                     /translation="MKKIREKLRQVLSHDIGIDLGTANTLVYLKGRGIVINEPSVVAV
                     NQKTGQVVAVGAEAKMMLGRTPAHISAVRPLVDGVISDFEVTEEMLAYLLSKAQENSR
                     KLMAPRVVVGVPSGITSVEIRAVRDAAVSAGAGVVHIVEEPMAAAIGMRLPIHDPVGS
                     MVVDIGGGTSDIAVVSLGGMVRSKNLRIAGDRFNTDIINYIRNQFKILIGEKTAESVK
                     FVATALIPLDVTLEARVKGRDLVTGLPREVIITDADVREALSQSIESLVIAIREVLEA
                     TPPEVLADIIERGIFLSGGGALLRGLPEVLETELGIKIHVASDPLSAVARGTGVILED
                     LEKYESILIENEDELPQKL"
     gene            complement(8123..>9040)
                     /locus_tag="PLD99_02360"
     CDS             complement(8123..>9040)
                     /locus_tag="PLD99_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRH25773.1"
                     /translation="TNLKDGAEVGLAFTHEEPISNMLEQYVSSYKDLPFSVYQFQNKF
                     RNEARAKSGIMRTREFVMKDLYSFAKNEAEHQDFYNKAAEAYKNIFKRVGLGEVTYMT
                     RASGGSFSEFSHEFQTITNAGEDVIYITDEDKKEAINKEIAKGNEGREVKAVEVGNIF
                     TLGTKFSDPNLTYKDESGESKPVFMGSYGIAPSRTMGTIVEVLSDDKGIVWPKAVAPF
                     RVHIISIGQDELATKLYETLSKDIEVLLDDRDASAGEKFADADLIGIPTQIIIGKKSV
                     ESGKFEVKDRRSGERSEIGETELVEFLRN"
BASE COUNT         2362 a         2361 c         1869 g         2448 t
ORIGIN      
        1 gggagatttc ttcgaactcg atcggaagta tgaactcctt cgacttggtg tcgaagagac
       61 catatttcaa atccttacac accatgtaga aagttctgtt gaggtgggag atccggtcgt
      121 aattctgcaa caactgcaaa agttgatcct tcaaagtact tctcattttt tccataagca
      181 ctcctaggct tgatgtgaga aatgtttgtt cttctgataa actacaataa caaaagtttt
      241 gtgtcaatag ttaataaaaa aagcgcgggt tccttagaat tcgcgcccct gtgttcaaaa
      301 catctttact tcttatctcg aaacacgagt tgtactccga gcgccttact cgatgtcttg
      361 gtctcgttgt cgtacatgaa gggtgagagc cagagctgaa gcgggatctt gtcggtgaga
      421 gtaagcacca gtctcggacc cgtccccaca aacctttggg agaagatgcc aagcgctatc
      481 ccatcaaccg gccgacacaa gaggtcagcc tgatgccacg ggttagcaac tactctgcct
      541 ctctcgtaat acacctcgag tgtgcaggtt ctgccactga agagaagaag gaatgccgcg
      601 taacgctcgc tcttcttctc ctcacctgac acagactcaa gaccagctcc caggccaagc
      661 tcgaaccaat ctgtgagatc gtagtagagt ccaccaacga gcgagctata accagtctca
      721 ctgtaggcga atccccacac ccctgtccta ccatgaacct tcgcccaggt gtcgatgtag
      781 ttgttgatcg agctgagatc gattccgaga gagtcagcct tcttcacccg ctgatgatag
      841 aggccgaact cccagtcctg tgcctcgagc ggagaagcaa taagcaagaa gagcacaaac
      901 aatctgaaga acttcatttg ccctccaaga ggttgaaata tctaaaaaca gaatatcaca
      961 aattatacaa aagtcaattt agcataaagg gcggaggact caagaatgaa tcccccgccc
     1021 ttgtggtgcc agtcgccgtt actttggcga ccaggtcata cccatgtaga acatccactc
     1081 ctcggcgact gtgttccaag tactgttcgc cgtgaaggtc atccgctcgg catacggcgc
     1141 cgagatcgag atccctggga gccggttaaa cggatcgtcg aggaagtcga gcatggcgta
     1201 ggtgagcttc accctgtccg tgccgatcga catcataccg aagttgccga aggcgaagga
     1261 ctcggggttc ttcaccccat cgttcttcca gtcactcggt tcccagacga agaccgctgg
     1321 ccaatagagg ccactgaccg agatggaacg cgtgatcggc tgattgacgt agacatacgg
     1381 accgacccac ggcgctccct ggaagtagcc gatggtgctg ccgatcgtcg ccttggtctt
     1441 cgtgccgtag ttccgcgcga atgtgagcca cgcctgctga ggctggatgg tgaagtcgcc
     1501 gtagcgcttg ccatcgttcg agccgaagat cgccgtaccg acgatcccac tcgcgatcgc
     1561 atcgcggcct gagccgacgg tgatcgactg gagggacatg gcgctcggat tctgctgagc
     1621 catcgcactc gtgccgagca tgctcgtgag caggatgaag ctgaggaaac ggacgaacca
     1681 gcgcatcggg actcctgttg ctgaggtttg gtgttctgaa ggttcaacta ctatataaat
     1741 gatagcacac tatgtacacc ttgtcaaatt atttaatgca gaaagggcgc aagactctcg
     1801 aaagaatcct gcgcccttgt tgatcacgga gtgatcctca gaacgaacaa tgaattactc
     1861 gctccgcgac tctcgatgtc ccaagcgatc ggaacaacga tcagggtcac agccttcgtg
     1921 gtgacctgaa gacgtggacc gaggccgatg aaacgctgga ggcggaagcc gacaccgata
     1981 ttggtctgag gtagtgggat gttcccgtcc accagatacc agtgtccact acccccttgc
     2041 tcgacagcag tgaagagcga gcccttgctg ttgccgatcc acaccctacc cattgcacgt
     2101 agcggattgt catgcttctc gactccggca ccgaagccga actcgatcca gttcttcggc
     2161 gagtatgtca ggccggtgat cgattctccc cagttctggc tgacgagaga ccagatgaac
     2221 atgccagtct ttggcgtgag cttgatgctc cccgagatgt ccatctgcgg gaatgatcca
     2281 ccgtcgcccg tcacgtacac ggcttcggtc cagaaagagg cttgggcctc cgcacggaac
     2341 gagatcgtga tcatcaggat gaaaagaaga atgaaacgca agtgatcctc ctgtctatgg
     2401 gtcaaagacc cgttaaagta cgacaaacat atttctactc taataatagc acaaattata
     2461 ccactttgtc aagtagtatt tagtccctat ttttaggctt gagagttggg aagagaatta
     2521 cttctttaat attagatact cctgtaagaa gcatcactag acggtctatg ccgataccga
     2581 taccaccgtt aggaggcata ccatactcca ttgcttcaag gtattccata tctgatggag
     2641 agatgtcttt gttgccaccc ttacccttct tgtcctcttc taggtatctc tctttctgat
     2701 ctattggatt attaagctct gagaaagcat tcacaagttc cgcaccacag atcacaagtt
     2761 ggaaacggtc gataagagtt gggtcttcct ccttcctctt cgcaaatgga ttgaaggcta
     2821 caggataatc aatgaggaat gttggttgta tgagtgttgg acgcaagttc ttcttgtact
     2881 cttcgtcagt agtatcggga aacttcatta ctttgaaatc tggttcgaga tcaataattt
     2941 gtccttcgta ttcaaattgg agtttgccga aaatcccctt aacaacatat ctaaataatt
     3001 cttgaataaa ctctctttga gacaccgcgt cctcgtatgc tgagttcgac tcaagcatgg
     3061 tgaactctgg attgtgggtc gagtctatcc cctcgtttct aaatctcttg cctatctcga
     3121 agaccttggg cataccacca gccaagagct ccttgaggta tagttcttga gcaataggta
     3181 gataaaagtc catgtcgagt gcgttgtgat gcgtggtgaa gggtgctgca gtcgcaccac
     3241 cagcaagagg ctggagattt ggtaggtcca cctcgacata accatgcttc aagtagaact
     3301 ctcgtatcaa agaaacaatt ttcgatcgag taaggaatct cgctttgacc tctggattag
     3361 aaagtatgtc caagtatctc ttgcggaatc tctcttcgat atcagtaaga ccggcccact
     3421 tctccggtaa tggcctcaaa ctctttgcta gcatgcggag accttctact agcaatgttt
     3481 tttcattttg ctttgttaca aaaagttcgc ccttgatctc aatgaaatct ccaatatcaa
     3541 aatactcact aaataattca aaatcctcat cctttgttgg atcacccttc ttgagtagtg
     3601 cttggaattt acccgtacca tcgtcgatat ctatgaaaat cactttccct tgtcctcgaa
     3661 ttgccatcac tctacccacg agttgcaccc tttctccctc cacaaaatcc ttagctgcgt
     3721 cagctaaagt tttatcgaaa tgagaggtag ttgggtacgg attaataccc ttctcgataa
     3781 gtgcttcgag tttttttata cgggtttctc gtatttcgtc tatacttgcc acaagtctat
     3841 tctatatcaa tgaccttata ggtcatagtg ccattaggag ttactacaga aaaactttct
     3901 cccttcttct taccaagagc agctacacca attggagatt taaccgaaat cttaccactt
     3961 gctgcatcag actcctcgga accaacaatg gtgaaaatct ttttggtacc atcatctttc
     4021 ttcaaagtaa caacacctcc cacagagaca gagtcggtat tattgttgga gacaatagta
     4081 gcgctacgaa gcaaatgctc gaggtagata atacggtctt caacaagggc ctgagcattc
     4141 ctagcctcgt ggtattcggc attctctgag aggtcgccaa gacttttagc atactcgagt
     4201 tgcttagcaa tctcggtcct cttctctgtc tttaagacat ctagctctgc cttgaattcg
     4261 ttgtacttcg cttgggttaa gtattccatg tggttaaaat tatctcgaag atgatatcag
     4321 aaattgaccc aattttcaac ctgactagtt caagtattcc ggagtgttct cgaccatcca
     4381 gtctaggacc tggcgcatga catatacacc cccgacagtg ttctttacag gacctttctc
     4441 cattatgacc acaaaagcat acctggggct ctcgtaaggg aagaagccaa caacccaagc
     4501 attcacaaac tcctttctta aaccaagctc agccgtacct gtcttacctg caaccttaac
     4561 ctctggtttc gataatcccg aggcagtacc cgcagtcacc gtttgtctca taccctctct
     4621 aactacatca aagtattcct tttttaagtc tattgttctt gtcaccttgg cctctgtggt
     4681 ctgtccaccc aggaggactg agggttttag catctttcca ccattcgcta ttgctgccac
     4741 gaatctaaca gcactaatag gtgtaatctg tgtaccatac tgaccaatcg aagtgtggta
     4801 agtattacct actcgccata ctgggtcatc agggaagttg atctccttcc acgaaggtgt
     4861 agcaatgtac cccaatgact cactcggtag gtcgataccc gtcatttcgt ttaatccaaa
     4921 aagggagaag tattcatcta gcttagttat acccaatcct ttttgagtac caaatccgcc
     4981 ccccacttca tagaagtaaa catccgatga catagctatt gcctttctca tatcgaccag
     5041 accatgtgcc ttccagtcct tgaaaatact tggtttgtcg gggttatatg ggttgggtag
     5101 tgtgagcgct ccggtagaaa gaatttcttt gtacgggtct ataacaccct cattcaaagc
     5161 acctagtgca acaatcggct taactataga acctggggta tagagtccag agacaacacg
     5221 gttaagaaat ggagtacgct tgtcgttaag gagtttgttg atctcaggct tattggcacc
     5281 ctctgcaacc aagttctgat cgtactcagg gtaactagtt agagcaagga tctccccggt
     5341 gttcacatcc attatcaccc ccgccccacc agtgaagcct ttctcattcg ccagactttc
     5401 tatagtttta tacatcttct cattcaaacg actatccaca gaaagaaaga tatctccacc
     5461 cttctttggc aggagcacca ccgactctga agtaatgtca cccaatgcgt ctgtctcaac
     5521 caacttgcta ccattctccc cttttagtat ttcgttatat gttttctcta caccaagctc
     5581 gccacgataa gtctcgtcgt agtacctgcc cgaagagtcc ttgagtggat gcttaacgta
     5641 accaacaaga gcgctcatgc ccttaatatc tggatacttc ctaagagcaa agtctgaact
     5701 agtcgcatac aaaacattct ctgctagagg tactgagttc ctgtcgaaga tcacacccct
     5761 gtcagagaaa ataatgtaac gggacaatct attttcttcg ctcacctgct tgtacaaact
     5821 tccgtcactt acctgtagcg cccaaagctt agtggaaagg aggagaaaaa caagaaccag
     5881 agcaacctta aataggagga aggtattctc cccgattggg ctctccatcc ttccttcgaa
     5941 acggtctcga tttaacccgg gcaagttagc cgagtcgatg aaaatgtctt caggatttat
     6001 ctccttgttt ctgcctctac cactcttcct aattcgtcgt aagaaattat gcatggaatc
     6061 tcaaatattt ccttagcggt gtgacaaaaa taatagctac ggtggctgaa aaaccaaata
     6121 tataaccaaa gagtggcgca ccagccctag ccccataaag agtgtctata agcaaccctg
     6181 caactatacc ttccaagtaa tacgggtgta aaaagattgc aataaacaat actgacaaat
     6241 agacccatgc tggagcgaag actattgata gaccaagcat tattgtagtt agggtcctca
     6301 tgttatctga cttctacaaa cctgatctta tgcatattaa caacactcct ggctaaaact
     6361 ttttttaaag aatctgtagg ctcgataaac gatgcctcaa ccactgcgac aagcggcgca
     6421 tcaatacttc cgaggagaat cctgtctcct ggcatgacca atacatcctt aggaacagta
     6481 aagaagaatg ttcccccgcc tctaccaatt agagtcactg gttcatcatc tcgatccaga
     6541 aaagccgaag tctctctacc gctcgtacta tatagagaga ccttggagtt aaactgactc
     6601 acctctacta cagtacccag ttctcccaat acgggatctg acactctggc cccatctctt
     6661 atgccgttta cctcaccagc atcaagaact aatatgtcat acaaggtctt tggcggtctc
     6721 gctattactg tcgcagcaat gaagtcgccc accccattcc ttccaaagag ttctagcatc
     6781 tctgtctgag tatcctcata gttcctcacg gatgactcaa gactatcaag taagaccaac
     6841 ttttccttaa gcagaatatt ttcttctacc aacgcattct tatctttaat cattagggaa
     6901 agctctttga aacttctagc caagaagttc tgcccctgcc aaagtgggga aaccaatccc
     6961 aagattggac ttttaaataa tgtaacaaca agtgccatga atatgaaggc accaaaaata
     7021 tatgtgagtc tcctctcttt atctctattt attctcctat aacttttgtg gaagttcgtc
     7081 ttcattttct attaaaatac tttcgtactt ctctaagtcc tccaagatga cacctgtccc
     7141 tcgtgctaca gcagaaagtg ggtctgaggc aacatgaatc ttgataccaa gctcggtctc
     7201 taacacctca ggcaagcctc taagtagtgc cccaccaccc gaaagaaaga tccctctctc
     7261 gataatatcc gccaggacct caggtggagt tgcctcaagc acctcgcgta tagcaatcac
     7321 gagactctct atagactgac tcagagcctc tcttacatct gcgtctgtta tgatcacttc
     7381 gcgtgggaga cctgttacca agtccctccc cttcacccta gcctcgagag taacgtcgag
     7441 aggtatgagg gctgtggcaa caaacttcac cgactcggcg gtcttctctc ctatcaatat
     7501 cttgaactga ttcctaatgt agttgatgat gtctgtgttg aacctgtcgc cagcgatgcg
     7561 caagttcttc gatctgacca taccccccaa tgagacaaca gcaatgtccg aggtaccacc
     7621 accaatgtcg accaccatgc tacccactgg atcgtgtata gggagacgca taccaatggc
     7681 tgctgccatg ggctcctcta ctatatgcac tactcctgcg cccgcactaa ctgccgcatc
     7741 tctaaccgct cgtatctcaa ccgaagtgat accagagggt acaccaacaa ctactcgagg
     7801 agccatgagc ttcctcgagt tctcttgagc cttgctgagt aagtaagcaa gcatttcctc
     7861 ggtaacctca aagtctgaga tcacaccatc aacaagaggt ctaacagcag agatgtgtgc
     7921 gggtgtacga ccgagcatca tcttggcctc ggcaccgacg gctactactt ggccagtctt
     7981 ttggtttact gcgacgactg agggttcgtt aattactatc cctctacctt tgaggtagac
     8041 caaagtgtta gcagtaccta agtcgatacc tatgtcgtga ctaagcactt gcctcagttt
     8101 ctcccgtatc tttttcatca tgttagttcc ttaagaattc aaccagctca gtctcgccta
     8161 tctcgctcct ctccccactt ctcctatcct tcacctcgaa cttaccactc tccacactct
     8221 ttttgccgat aataatctgt gttgggatac caataaggtc tgcgtcagcg aacttctccc
     8281 ccgcacttgc atctctgtcg tctaggagta cttcaatatc ttttgagagc gtctcataga
     8341 gtttggtggc caactcatcc tgaccgatag atattatatg tacgcggaac ggtgccactg
     8401 cctttggcca gacaataccc ttatcatccg agagcacctc aacaatcgta cccatagtgc
     8461 gagacggagc tatgccatat gagcccataa atactggctt actctcacca gactcatcct
     8521 tataagttaa gttagggtca gagaacttgg tgccgagggt aaatatattt cctacctcta
     8581 cagccttcac ctccctacct tcgttcccct tggcgatctc tttgtttatt gcctctttct
     8641 tgtcttcatc agttatatag atcacgtcct cacctgcatt tgtaatggtc tgaaactcgt
     8701 gtgaaaactc agagaaagaa ccaccagaag cccttgtcat ataagtcacc tctcctaatc
     8761 cgactctttt aaaaatattc ttgtatgctt cggcagcctt gttataaaaa tcttgatgct
     8821 ctgcttcgtt cttcgcgaaa gaatacaaat ctttcatcac aaactctctg gtgcgcataa
     8881 tgccactctt ggcacgagct tcgtttctaa acttattttg gaattgataa acggagaaag
     8941 gtaggtcctt gtacgaagag acatattgct cgagcatgtt cgatattggc tcctcgtgag
     9001 taaaggcgag accaacttct gcaccgtcct tcaaattagt
//
LOCUS       DAORIT010000020         8387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_1485_length_8387_cov_49.661906, whole genome shotgun sequence.
ACCESSION   DAORIT010000020 DAORIT010000000
VERSION     DAORIT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1485_length_8387_cov_49.661906"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1175)
                     /gene="secF"
                     /locus_tag="PLD99_02365"
     CDS             complement(273..1175)
                     /gene="secF"
                     /locus_tag="PLD99_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRH25774.1"
                     /translation="MFVVKYRKVWFVISALLLGLSIYSFFSFGFKLSTDFIGGSITEV
                     KYIGEVPSKQLVQDEVRELNLGGISVRPSAGDRYVIRTQSLDSEETKKLTDALTINNE
                     FKSEIVRSNTIGPVAGAELQSKALKAILVVIIMIVLFITFAFRGVSKPVSSWKYGFAT
                     VIALVHDVLIPTGLFVLLGHLYGVEIDLLFVTGLLAILGYSVHDTIVVFDRVRENLKI
                     NGERNITEEFETTVGKSVSQTFTRSINTSVTIFITLLALYIFGSSATKDFSLLLLVGV
                     IIGTYSSIFVASPLLVTFFKSQRK"
     gene            complement(1177..>1800)
                     /locus_tag="PLD99_02370"
     CDS             complement(1177..>1800)
                     /locus_tag="PLD99_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HRH25775.1"
                     /translation="VRDLNYGALPVPINLVNSQTIGASLGEEALNAGVKAGIIGFIAL
                     SLFLIFWYRLPGLIAVMALAAYVVLSLTVFKLIPVTLTAAGLAGFILSIGMAVDANVL
                     IFERTKEELMKGKKISDALHEGFSRAWLSIRDSNTASIITAIILFWLGTSAVKGFALT
                     LGVGVVVSMFTAITLSRTFLFAIAPKDTGEAEGKFKRFLFSNGFHIK"
     assembly_gap    1801..1900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1901..2232)
                     /locus_tag="PLD99_02375"
     CDS             complement(<1901..2232)
                     /locus_tag="PLD99_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25776.1"
                     /translation="MKATKYRVWSIILLALFSLLFFRFLDSDSRPFNYGLDLVGGTEL
                     IYHADTSEVSDRTGAMDSLKEVIERRVNIFGVSEPLVQTEHAGFVSGLHDDRLIVELP
                     GVTDIEEA"
     gene            complement(2242..3531)
                     /locus_tag="PLD99_02380"
     CDS             complement(2242..3531)
                     /locus_tag="PLD99_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRH25777.1"
                     /translation="MQILGKKEPVILVIGDILLFVVSLWFSLLLRNLSIPTGTFFATH
                     LVPFSIIFLASVVSFYIGGLYGKHTGLLRSKLPNTLINTQIANSLIAVIFFYLIPIFG
                     IAPKTLLFIYLAISSSLIIFWRRYGYFFLVPRSSENAIIIGNAEIVKEIVSEVNTNNI
                     YNIRFVKNFDEASVVIADFSDENIKSILPDLYEQIFSGMTFIDIHKIYEEIFDKVPLK
                     LLNYNWFLENISTQPRVMYDSLKRAMDLALSLVLGAVSLLFYPFIYVAIKLDDGKDIF
                     ITQDRVGQGGRLIKIYKFRTMSTNDQGDYNSGNINTVTRVGAFLRKSRLDEFPQLWNV
                     IRGDISLIGPRPELPNLVKLYDKEISYYNVRHIIKPGLSGWAQIYQENHPHHKEAVDE
                     TKEKLTYDLYYIKERSLLLDLKIALKTVKTLLSRVGV"
     gene            3571..4674
                     /locus_tag="PLD99_02385"
     CDS             3571..4674
                     /locus_tag="PLD99_02385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRH25778.1"
                     /translation="MRILYGITKSNFGGAQRYVFDLALESKKLGHEVGVICGGEGTLV
                     DKLNEANIDVYNIQNFGRDFSPIRDLYGLFYFYGVLRNFKPEVLHLNSAKMSGAGIFL
                     GRLLRIKKIIFTAHGWAWNENRSWISKIIIKKLSWLTIIGSHRTIAVSKAVYDGISSW
                     PITKKKMVVIRNGVREFALLEHDIARNKLGVSNSDFIVGTISELHHIKGLDFLIEAWS
                     KFKNSRDGKLIIIGAGEEEENLKAQAKRLGVSESIYFKGFVDDARSLLKGFDIFTLTS
                     RSEGLPYAILEAGTAALPIIASRVGGIPEIIDGENTGMLINVGDVKQLETYLKDLSDS
                     EQRRAALGGNLHNKIENEFTLEKMVRDTLELYA"
     gene            4667..5122
                     /locus_tag="PLD99_02390"
     CDS             4667..5122
                     /locus_tag="PLD99_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25779.1"
                     /translation="MLNNFFISIDQTIISLVRKVSIPVSRIAIFIVYFWFGLLKVLGL
                     SPASPLVLSLLDRTMPFISPDAFLVWFGLFEVLIGTIFLIPKFARAAIALLVIHLITT
                     VMPLFLLKSSLWTGMMVPTLEGQYIIKNVLIVALAIVVASNLTPWRSRA"
     gene            complement(5096..6175)
                     /gene="rodA"
                     /locus_tag="PLD99_02395"
     CDS             complement(5096..6175)
                     /gene="rodA"
                     /locus_tag="PLD99_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRH25780.1"
                     /translation="MRKVFDWSLFVPALLISLSGLVTMNSFAHEGSYFNKQIIWLLVG
                     VFVFFLANAIDWRFLARTKVSVAVYLLCLGLLLLLFSLGVTANGAQSWFNFGGFAIQP
                     SDPAKLAIILILAKYFSRRHIEIANLRHIIISAIYTGVIAFLVFLQPDFGSAVIILGI
                     WFGMVLASGISKKHLLVVIGLGVLSVLSLWLFVFEPYQKARIATFLSPLSDIQGAGYN
                     AYQSTIAVGSGEILGKGIGLGSQSKLLFLPEYQTDFIFAAFAEEWGFVGTLFLLILAF
                     ALLYRLVDNAKNSGGNFESLFTLGVATLFGIHILIHAGMNMGLLPVTGIVFPFMSYGG
                     SHLLTEWFALGIISSMSLRSRAPRS"
     gene            complement(6177..>6962)
                     /locus_tag="PLD99_02400"
     CDS             complement(6177..>6962)
                     /locus_tag="PLD99_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25781.1"
                     /translation="MNPWAFKRKSITFGIFLLFVLLFVALPAYYFTRSSPKCTNLIQD
                     GDETGVDCGGSCALLCKPETLPLITRGEARINRISSSSYTASVLVENPNLSGEVKRAK
                     YNLSIYSSGVKDPVKVFNKETYIGPNSTFALFEGPFTLTGSGPFRAVFEWEDGLIWEK
                     NVVSSALIGIESPNLLITATSSQRLEARLINRSTKSISNVEVVAILSDIDGNTVASSK
                     TFVDFIEAGDSAPISFSWPEPFASVPVTTKILPHPLPDKSYIR"
     assembly_gap    6963..7062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7077..7183)
                     /gene="rrf"
                     /locus_tag="PLD99_02405"
     rRNA            complement(7077..7183)
                     /gene="rrf"
                     /locus_tag="PLD99_02405"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(7274..7379)
                     /locus_tag="PLD99_02410"
     tRNA            complement(7274..7379)
                     /locus_tag="PLD99_02410"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7311..7313),aa:Glu,seq:ttc)
     gene            complement(7467..>8387)
                     /locus_tag="PLD99_02415"
     rRNA            complement(7467..>8387)
                     /locus_tag="PLD99_02415"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2494 a         1855 c         1628 g         2210 t
ORIGIN      
        1 ttacatcttc ctcccaatgg gtaggcccca aggaagccga cctcccgcca agacagttcg
       61 tttggtgccc ttcaggttaa gcaccgggta actatcgtga ccttgggtta actcattagc
      121 tgccacttcg ggtagctaag cgatagaagc cacccacttc tgggatcggc ggtgcgaaag
      181 gtgtatgtac caggtacacc tgggaagatt aacgttcggt cgaaagactg gatacaccca
      241 cgctgcatac cactcttaaa acgagtggtt ttttatttcc tctgagattt gaagaaggta
      301 acgaggagtg gtgaagcgac gaagattgag gagtaagtac ctatgattac tccaactagt
      361 agaagaagag agaagtcctt ggttgcagat gatccaaaga tgtagagagc aaggagagtg
      421 ataaatatgg tcacagaggt attaatagaa cgagtaaatg tctgggacac agacttacct
      481 accgtagtct caaattcttc agtaatgttc ctctcaccat ttatctttaa gttctcacgc
      541 actcggtcaa aaacgacaat cgtgtcatgc actgagtatc caagaattgc gagaagcccg
      601 gtaacgaaaa gtaagtcaat ctcgactcca tacaaatgtc caagcaaaac gaagagtcct
      661 gtagggatca aaacatcgtg taccagggcg ataactgtag cgaaaccata cttccaagat
      721 gaaacaggtt ttgaaacacc tctaaaggcg aaggtgatga agagcactat cataatgata
      781 accacgagaa ttgccttcag cgccttcgat tgtagctctg ctccggccac tgggccaata
      841 gtgttcgaac gaactatctc actcttaaat tcattattaa tcgttagagc gtcagtgagc
      901 ttctttgttt cctcagaatc aagtgattga gttctaataa catatctgtc accagctgat
      961 ggtctaactg atatacctcc aagattcagt tctcttactt catcttgtac aagttgttta
     1021 cttggaactt ctcctatgta cttaacctct gtaatagaac ctccaataaa gtcagttgag
     1081 agcttaaaac caaatgagaa aaagctatat atagagagtc caagaagtaa cgccgagata
     1141 acaaaccaaa cttttctgta ttttacgaca aacatattat ttaatgtgga acccattact
     1201 gaataaaaat cttttgaact tcccctctgc ttcaccggta tcctttggcg caatggcgaa
     1261 aaggaatgtc ctactcaaag taatcgcagt aaacatagat acaactaccc ccacacctag
     1321 tgtaagcgca aagcccttaa ccgctgatgt accgagccag aaaagtataa tcgcagtaat
     1381 gatacttgct gtatttgagt cacgaattga gagccatgct cgagagaatc cctcgtgcaa
     1441 ggcatcactg atcttctttc ccttcataag ctcctccttg gtacgctcaa agataagcac
     1501 attcgcatcc actgccatac caatagagag aataaaccca gccaatccag ccgcagttag
     1561 tgtgacaggt atcaacttaa agacagttaa agatagaaca acatatgcag caagagccat
     1621 aactgcaatt agtccaggta gtctatacca aaagattagg aacagtgaga gagctataaa
     1681 accaattatc cccgccttca caccagcatt aagcgcctcc tctccgagtg aggcgccaat
     1741 agtttgacta tttaccaaat taataggtac aggcagcgca ccgtaattaa gatctcttac
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttgcctctt ctatatctgt
     1921 cactcccgga agctcgacta tcaacctgtc atcatggagt ccagagacaa agccggcgtg
     1981 ctctgtctgg acgagcggct ctgatacacc aaagatattc accctccttt caataacctc
     2041 cttaagactg tccatcgctc ctgtcctgtc actcacctca cttgtatcag catggtagat
     2101 tagctctgtt cctccaacca agtctaaccc gtagttaaaa ggtcttgaat cactgtccaa
     2161 gaacctaaag aaaaggagac tgaaaagggc tagcaatata attgaccaga ctcggtattt
     2221 ggttgctttc atagcgagct attatacacc aactctagaa agtagggtct tgacggtttt
     2281 cagagctatt tttaaatcta aaaggagtga acgctctttt atatagtaga ggtcatatgt
     2341 tagcttctcc ttagtctcat ctacagcctc tttgtgatgc ggatgattct cctggtaaat
     2401 ctgagcccaa ccggaaagac ctggttttat gatatgtcga acattgtaat aagagatctc
     2461 tttatcatag agcttaacta agtttgggag ctctggtctt ggaccaataa gagagatgtc
     2521 tccgcggatg acattccaga gttgtgggaa ctcatctaac ctagactttc taagaaaagc
     2581 tccaactctt gtaactgtat ttatattccc gctattataa tctccctgat cattagtact
     2641 cattgttcta aatttataaa tcttaatgag tctacctccc tgacctacac gatcttgagt
     2701 tataaaaata tctttaccat catctaactt aatcgcaaca tagataaacg gataaaagag
     2761 taaggacact gctccgagca caagagagag cgctaagtcc atagctcttt ttagagaatc
     2821 gtacattact cttggctgag tagaaatgtt ttcgagaaac caattgtagt taaggagctt
     2881 taatggcacc ttatcgaaga tctcttcata gatcttgtga atatcaataa atgtcatacc
     2941 agaaaaaatc tgttcatata aatctggcaa aatagatttg atattttcat ctgaaaaatc
     3001 agcaatgaca acagatgctt catcaaagtt cttcacaaat cggatattat aaatattatt
     3061 tgtattaacc tcactcacta tctccttaac aatctctgcg ttgccaataa tgatcgcatt
     3121 ctctgaactt cttggcacga gaaagaagta cccgtatctc ctccaaaaga tgattagtga
     3181 tgacgatata gccaaataaa taaagagcaa ggtcttcggc gcaataccaa atattggaat
     3241 gaggtagaag aatataaccg caataagaga attcgcaatt tgggtattga tgagagtgtt
     3301 tggtaattta gagcgtagaa gtccagtatg cttaccataa aggcccccta tgtaaaaact
     3361 cacaactgaa gctaggaaaa taatagaaaa cggcaccaag tgagtggcaa aaaaagtacc
     3421 agttgggatg ctaagatttc ttagcaagag tgagaaccac agtgagacaa caaaaagcag
     3481 tatgtcccca atcaccagta ttacaggctc ctttttgccc aaaatttgca ttgtatatac
     3541 attataacac aaaaaagtta taatgactat atgcgtattc tatacggtat tactaaatcc
     3601 aatttcggtg gagcacagag atatgtcttc gatctcgcac tagaatctaa aaagcttggt
     3661 catgaagtag gagttatctg cgggggcgag ggcaccttgg tagacaagtt gaatgaagcc
     3721 aatatagatg tttataacat tcagaatttt ggccgagact tctcccccat tagagatcta
     3781 tacggtctat tctatttcta tggagtacta agaaacttca agccagaggt tctacacctt
     3841 aacagtgcca aaatgagtgg cgcgggcatc ttcctcggca gattactcag gattaaaaaa
     3901 atcatcttta ccgcgcatgg ttgggcgtgg aacgaaaata gatcttggat ctccaaaata
     3961 attatcaaaa aactttcttg gcttactatt atcggttcac accgaactat tgctgtctca
     4021 aaagcagttt atgatggtat atcttcctgg cctatcacta agaaaaaaat ggttgtgata
     4081 aggaacggtg tgagagagtt cgctctgtta gaacatgata ttgcaagaaa taaactcggt
     4141 gtcagcaaca gtgattttat agttggcaca atatccgaac ttcatcatat taaaggtcta
     4201 gacttcctaa tcgaagcttg gtcaaaattc aaaaacagta gagatggcaa actaattatc
     4261 attggcgcag gcgaggagga agagaactta aaagctcaag ccaaaagact tggagtatct
     4321 gaaagtatct acttcaaagg ctttgttgat gacgcgaggt ctctacttaa aggttttgat
     4381 atatttactc ttacttcaag atcagagggt ctaccctacg ccatactcga agccggcacc
     4441 gccgcccttc ctatcatcgc ttcaagagta ggtggtatac ctgagattat agatggtgag
     4501 aacaccggta tgttaataaa tgtaggagat gtaaaacaac tagaaactta tttaaaagat
     4561 ctcagtgatt ctgaacaaag acgagctgct ctaggtggca atctccacaa caagatcgaa
     4621 aacgaattca cactagagaa aatggtgcgt gatacactag agctatatgc ttaacaactt
     4681 ttttatctct atagaccaga caattatttc tttagtaaga aaagtttcta taccagtctc
     4741 tagaattgca atttttatcg tttacttttg gtttggtcta cttaaagtat tgggtcttag
     4801 tcctgcaagt cccctggtct tgtccctcct tgaccgcacc atgccgttta ttagccctga
     4861 cgcctttctc gtttggttcg gattatttga ggtcttgata ggcaccatct tcctcatccc
     4921 taaatttgct cgtgcggcca tagctcttct cgtcatacac ttaatcacca cagtaatgcc
     4981 cctattctta ctcaagagct cactctggac tggaatgatg gtcccaacac ttgaaggtca
     5041 gtacattatc aaaaatgttt taatagtagc tttggctata gttgttgctt ctaacctaac
     5101 tccttggcgc tctagagcgt aagctcatac tagaaataat ccccagtgca aaccactcgg
     5161 taagcaagtg agaccctcca taactcataa atggaaaaac gattcctgtt actggcaata
     5221 accccatgtt cataccagcg tggattaaga tatgtatacc aaaaagtgtc gctaccccca
     5281 atgtaaagag tgattcaaag ttcccccccg agttcttggc attatctact aatctataca
     5341 agagagcaaa agctagtatt agaagaaaaa gtgtaccgac aaacccccac tcctctgcga
     5401 acgccgcaaa aataaagtcc gtctgatact cgggtaagaa gagaagcttc gactgacttc
     5461 caagtcctat ccccttaccc aagatctcgc cggaccccac agcaatcgtt gattgataag
     5521 cgttgtaccc cgcaccctga atgtctgaga gtggcgagag gaaagtggca atacgagcct
     5581 tctgataagg ttcaaagaca aaaagccaca agcttagcac cgataaaaca cccaacccga
     5641 tgacaaccaa taaatgtttc ttagaaatcc ctgaggccaa gaccatacca aaccaaatcc
     5701 caaggataat aaccgcacta ccgaagtctg gttggaggaa tacgaggaag gcaatgactc
     5761 ctgtataaat tgccgagata atgatatgtc tcaaattagc aatctcgata tgacgcctag
     5821 aaaaatattt tgccaaaata agtattatgg cgagcttcgc tggatcagag ggttggatgg
     5881 cgaaacctcc aaaattaaac caagattgag cgccgttcgc agttactcca agagagaaca
     5941 agagtagaag caatcctaaa caaagtaaat acactgccac tgacacctta gtcctggcca
     6001 aaaacctcca atcaatagca tttgccaaga agaagacaaa tacccctacc aaaagccaaa
     6061 tgatttgctt attaaagtaa gacccttcgt gcgcgaagga attcatagta acaagaccag
     6121 acagtgatat gagtagcgct ggcacaaaaa gtgaccagtc aaaaactttt cgcatattat
     6181 cttatataac ttttatctgg gagaggatgt ggcaggattt ttgtagtcac tggtactgag
     6241 gcaaatggct ctggccatga gaagctgatt ggtgcagagt ctcctgcttc gataaagtct
     6301 acaaaggttt tgctcgatgc tactgtgttg ccatcaatgt ctgagagtat ggctaccacc
     6361 tccacattgc ttatactctt agtagagcga ttgataagtc ttgcttctag tctctggctc
     6421 gatgttgcgg taataagcag gtttggactc tcaataccaa taagtgcact gctaactaca
     6481 ttcttttccc aaattagacc atcctcccat tcgaaaactg ctcgaaatgg gcccgatcca
     6541 gtcagtgtaa atggtccttc aaaaagagcg aaggtgctgt tcggtcctat gtaggtttcc
     6601 ttattaaata cttttacagg atccttaact cctgaagaat atatggacaa attatacttc
     6661 gctctcttca cctcgccaga aagatttgga ttctctacca aaacagaagc agtgtaggag
     6721 ctcgaagaga tacggttgat acgagcctca ccacgagtga taagcggtaa ggtctcgggc
     6781 ttacaaagca gtgcacacga cccgccacaa tctactccgg tctcgtctcc atcttgtatc
     6841 aaattagtac acttaggact acttcgggtg aagtagtatg caggtagtgc gacaaacaaa
     6901 agtacaaaaa gcaaaaagat gccaaaggta atactcttcc tcttaaaggc ccaagggttc
     6961 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttcaaac gctatgttcc
     7081 ggcgactacc tactttcccc cagaggagta tcatcggctc aacgaggctt aactgccgtg
     7141 ttcggaatga gaacgggtgt gaccctcgcg acaaatcacc agaacataat tttcgaaatt
     7201 gatgtggaat gctttgagtt agggcgaaca tcttgttcgc cggtgaaagg acaatgttgg
     7261 aaaaaacaat acatggggcc gaaaggattc gaacctctgg cctctgcagt gaacgtgcag
     7321 cgctctacca tgggtatgtg ctcttcagct gaagcactct cccgctgagc tacggcccct
     7381 cataaccaac tcaaaacatt ccacaaaact aattaacaat tgatttcatt tagcaggaca
     7441 gagttacctc tttgtgactt aaaagtttcc taataagatt cgagcaatta gtacgtctcg
     7501 gctcaacacc ttacgatgct tacacctaac gcctatcaac ctagtcatct cctagggctc
     7561 tcaacgattc cttatcttgg ggctggcttc attcttagat gctttcagaa tttatccatt
     7621 cccgacttag ctacccagcg gtccacttgg cagtagagct ggtacaccag tggtcagttc
     7681 aactcggtcc tctcgtacta gagtcaaatc ccctcaagaa tcgaacgcct gcagtagata
     7741 ggagaccaac ctgtctcacg cttgtttact ctcatgagtt tccatccttt ccaggatgca
     7801 atggactata tcttcacccg aacattcgaa aatgttttag ggggctaacg tgtagtctct
     7861 acgggcctcc atagaagttc cctcggtgtt gtcctttcgg aggatttcac cgatattagt
     7921 tagcagtatc aacgagaatt acttctcgcg atcgccgtga ttgtggttgg tttaccattt
     7981 ttttatcttt tcaagaacga aaaatttcga ttggttcaga ctgtacatgc cacgaatcaa
     8041 aagactcatg atctgaacgg tctgaaccca gctcgcgtaa cattttaatt ggcgaacagc
     8101 caaacccttg gaagcttctc cacctccagg atatgttgag ccgacatcga ggtgccgaac
     8161 tgcgccgtcg ctatggacgc ttaggcgcaa ccagcctgtt atccccagag tagcttttgt
     8221 ccgataatct ccggccgcat ctaacgcgga ccgtcggttc actatgttct gctttcgcat
     8281 ctgctcgaca agtacgtctt acagttaagc cagcttatgc cattacacta tcggcacggt
     8341 ttccattcgc gcctagctga ccttaataaa ctcctccgtt acttttt
//
LOCUS       DAORIT010000021         7603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_1721_length_7603_cov_55.993111, whole genome shotgun sequence.
ACCESSION   DAORIT010000021 DAORIT010000000
VERSION     DAORIT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_7603_cov_55.993111"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..84)
                     /locus_tag="PLD99_02420"
     tRNA            complement(2..84)
                     /locus_tag="PLD99_02420"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Tyr,seq:gta)
     gene            complement(97..2769)
                     /locus_tag="PLD99_02425"
     CDS             complement(97..2769)
                     /locus_tag="PLD99_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRH25782.1"
                     /translation="MPPFNHFTTKAKEAIRRAHELAIERGQNHVNPIHLLAALILQEE
                     SMVISIIDKLDIDSILLTDNVLEAIESRESSQTLSPSYQIYLTPELANIIDTSSKISQ
                     GLGDEFVSTEHLFLSVLESPGEAKELLTKFKIDKATVIRIIEELKKSGTAEPKTEKKF
                     KSLIKYTRSLTKLAKLNKLDPVIGRDQEISRIIQILSRRTKNNPILIGEAGVGKTAIV
                     EGLAIRMANGNVPESLRDKELVSLDLGLLIAGTKYRGEFEERLKNIIKEIEKSEGKII
                     LFIDEIHTIVGAGAAEGSIDASNMMKPPLARGELRAIGATTIREYQKHIEKDPALTRR
                     FQPVYVNEPSVEDAMTILRGLKERYELYHGVHITDDAIRAAVTLSSRYITDRFLPDKA
                     VDLIDEASSFLKISLENMPPILQETHSKVMKLEIEREALKRETKNPKVTARLKVIDKE
                     IADLREKTSEIELKWKNEKETVGEIKNIKKMLETTRLEAESAEATADLARAAELRYGQ
                     IPELERDLDTKAKRLKKLQSSRRILKEEITESDIADVVSRWTNIPVSRMLEEEAEKLG
                     RMEEYLKRRIVGQDEAVKKIADTVKRSRAGISDPQRPIGSFMFLGPTGVGKTELTKAL
                     AEFMFDDDKALIRVDMSEFMEKHSVSKLIGAPPGYVGHEEGGTFTETVRHRPYSVVLF
                     DEIEKAHPEVFNILLQVLDNGRLTDAKGRVVNFKNTIIIMTSNVGAQYIDKMQSIGFS
                     KGEKESYEGVKDKVLDALKDQFRPEFLNRLDDIIVFDVLTQENIKNIVNIQVDAVKKR
                     LQDKEIELVISDAVLTELAKEGYSPQYGARPLKRLIQTKILNPVATMMISNKILSGGT
                     VLVDKKGGEYTFDVKKGRRSGSVSAMVDKQRTTR"
     gene            complement(2788..3576)
                     /locus_tag="PLD99_02430"
     CDS             complement(2788..3576)
                     /locus_tag="PLD99_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRH25783.1"
                     /translation="MTTMEDLTKTQIVLLTLLVSFITSIATGIITSSLLAEAPQGVTQ
                     TIDRVVERTVERVTTPSGGGTVKEVTIVKEGDAIVSAVENNTKAVVRIKSPNGADGVQ
                     RFYGLGVVVNKQGLVLSDRRDIIQTGNYTVVFSDGSELSATIHKVSYTGSMILFRLIA
                     DDSKLRSLSTVTLSESDPKLGQTVVAIEGSERNAINVGRILSLNIATGFAETDIKGIE
                     TAGGPLLNLTGELVGLKTSNTDLSITQGLYTSISSLKEFIANNS"
     gene            complement(3585..4655)
                     /locus_tag="PLD99_02435"
     CDS             complement(3585..4655)
                     /locus_tag="PLD99_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRH25784.1"
                     /translation="MGILKRARIFLDFAGGRDNPSAIHEEGAGAKRILESSRERLARL
                     LKVQKKEVVFTSGGTESDNLAILGVFEAARVNMDKPHIIISSVEHSAVSESAKEVERR
                     GGEVTVIELEDGVLNVNTVLKSIKENTVLISIVYANSHTGGVSELPKIARLVRDIRSK
                     NNSKYPLVHTDATQALGYLSVDFPKLGCDLVTLDTSKLLGPKGVSALIVRSNVLIKPI
                     IFGGGQEFGLRSGTENVEAIQKFVEVVEECERDREKNFAKVSKLKELFLSEVKSLVPQ
                     AIINSPVGSLPSIVSLSMRGALHEFLVIKLDVKGVAVSTGSACLFRKSDGDTDAIRFS
                     FSPKTTESEIKKVVRILKDVVL"
     gene            4716..6212
                     /gene="infB"
                     /locus_tag="PLD99_02440"
     CDS             4716..6212
                     /gene="infB"
                     /locus_tag="PLD99_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRH25785.1"
                     /translation="MKSRRPPVVAIMGHIDHGKSTLLDYIRKSNTVDKEAGGITQHVS
                     AYEIVHTDGDGIMNKITFLDTPGHEAFNSIRARGAKAADIGVLVVSAEDGVKPQTIEA
                     LKRIREANVPMIVAITKIDKPSANIEMAKQSLAENDVFVEGYGGDVPVVALSAKSGVG
                     INDFLNLVLLLAEVENCTGEEDRLGSGVIIESRLDPKKGITAVGVLKDGTIKTGQVAA
                     SIGTIAPLRFILDATGTKIDSLTFSSPVQIIGWDRLPLVGATFEIFENKPAAQFYADS
                     EAIKAHIPKSEMSQANTEDGSISLPIIIKADAAGSLEAIKYELSKINKDKISIKIVSA
                     DVGTISEGDVKTGMTTPGTIIFGFNTKIDPQAGALAERTETISIELYDIIYKLTERVE
                     ELLNEKAPKFETEEVMGRAKVLKCFSKTKDKQVLGARMESGLFEGKSNFRIIRREEEV
                     GRGKVKELQQSKVVVDSITEGSEFGMLVESKTEIAPGDHLERVVMVTK"
     gene            6214..6546
                     /locus_tag="PLD99_02445"
     CDS             6214..6546
                     /locus_tag="PLD99_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRH25786.1"
                     /translation="MRQIKHDKVEETIHREAAKFILENSGNSSLITVTRVTLNPSGKD
                     AIIYFTTLPESEEDTAKKFLDRKAPEFKDHMMHESRIGIIPHLRFQIDYGERNRQRLD
                     EIGKDIQA"
     gene            6577..6648
                     /locus_tag="PLD99_02450"
     tRNA            6577..6648
                     /locus_tag="PLD99_02450"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6609..6611,aa:Cys,seq:gca)
     gene            6674..6755
                     /locus_tag="PLD99_02455"
     tRNA            6674..6755
                     /locus_tag="PLD99_02455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6708..6710,aa:Leu,seq:taa)
     gene            6897..6967
                     /locus_tag="PLD99_02460"
     tRNA            6897..6967
                     /locus_tag="PLD99_02460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6929..6931,aa:Gly,seq:ccc)
     gene            6988..7401
                     /locus_tag="PLD99_02465"
     CDS             6988..7401
                     /locus_tag="PLD99_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25787.1"
                     /translation="MRESGEFPLIPEPAPQEETRANRLESSDLNISEVEDYIKSTKFE
                     DKEELEDVLLKQFNMGKRESYSDIQLGMTRVIKAIGFGKDRRIIEDKPLSGKEPEADS
                     IIGIETIDGNKYYLMIFKVTNRNQKFRYEYILGAK"
     gene            7444..7516
                     /locus_tag="PLD99_02470"
     tRNA            7444..7516
                     /locus_tag="PLD99_02470"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7477..7479,aa:Lys,seq:ctt)
     gene            7521..7601
                     /locus_tag="PLD99_02475"
     tRNA            7521..7601
                     /locus_tag="PLD99_02475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7554..7556,aa:Leu,seq:tag)
BASE COUNT         2054 a         1795 c         1669 g         2085 t
ORIGIN      
        1 gtgcgccggg aaggattcga accttcgtag gccgaagccg acagatttac agtctgttgt
       61 aattgaccac tctaccaccg acgcgtagaa cctatattaa cgggtagtac gctgtttatc
      121 aaccatcgca ctaacacttc cactcctcct acccttcttc acatcgaaag tgtattcacc
      181 accctttttg tccacaagca ctgtaccacc agagaggatc ttgttgctta tcatcatggt
      241 tgccactggg tttagaatct tggtctgtat taagcgcttt agaggtctcg caccatactg
      301 tggactgtac ccttcttttg cgagctctgt tagtaccgca tcgcttataa ctaattcaat
      361 ctccttgtct tgcaaacgct tcttaacagc atcaacttgg atgttcacaa tgttctttat
      421 gttctcttga gttagaacat cgaagacaat aatgtcatct agtctgttta ggaattcagg
      481 acggaactga tccttaagtg catcaagcac cttgtccttc acaccctcat aactctcctt
      541 ctcacccttg gagaaaccaa tagactgcat cttgtcgatg tattgagcac cgacatttga
      601 ggtcatgatg ataatagtgt tcttgaagtt gaccactcta cccttagcat ctgttagacg
      661 accgttgtcc aaaacttgaa ggaggatgtt gaacacctct ggatgtgcct tctctatctc
      721 gtcgaaaagc acaactgagt atggcctgtg tctaacagtc tcggtaaagg tcccaccctc
      781 ttcgtgaccc acatatccag gaggagcacc aatgagcttt gagactgagt gcttctccat
      841 aaactcactc atgtcgactc ttataagtgc cttatcatcg tcgaacataa actcagcgag
      901 ggccttggtt agctcggtct tacccactcc tgttggacca aggaacataa atgagccaat
      961 aggtctttgt gggtccgata ttcctgcacg actcctctta acagtgtcag caatcttctt
     1021 tacagcctcg tcttgaccaa caatacgtcg cttcaagtac tcctccattc gaccaagctt
     1081 ctcagcctct tcctcaagca ttctcgagac aggaatattc gtccaacgac taacaacatc
     1141 agcaatgtct gactcggtaa tttcttcttt aagtattctc ctcgagcttt ggagcttctt
     1201 taatcttttg gccttagtgt ctaagtcgcg ctctagctct gggatctgtc cgtatctaag
     1261 ttctgcagca cgagccagat ctgctgtagc ctctgccgac tcagcctcga gtcgagtggt
     1321 ctcgagcatc ttcttgatat tcttgatctc acctacagtc tccttctcat ttttccactt
     1381 aagctctatc tcagaggtct tctctcgaag gtcggcgatt tctttatcaa ttaccttaag
     1441 tcgagcagta actttcggat tcttggtctc acgcttgagt gcctctcgct cgatctcgag
     1501 cttcattacc tttgaatgag tttcttgaag tattggtggc atgttctcga gtgagatctt
     1561 caagaatgaa gaggcctcat ctataaggtc tactgctttg tccgggagga atctgtcggt
     1621 aatgtagcga gacgataggg tgacagcagc tcttatggcg tcgtctgtta tatgcactcc
     1681 gtggtaaagc tcgtagcgct ccttaagacc ccgaagaatt gtcatggcat cctcgaccga
     1741 tggctcattt acatacacag gttggaagcg tcgagtaaga gcaggatctt tctctatatg
     1801 cttctgatac tcacgaatag tggtggcacc aattgcacgg agttctccac gggcaagtgg
     1861 tggcttcatc atgtttgagg catctataga accctctgct gctccagcac caacgatagt
     1921 gtggatctcg tctataaaga ggatgatctt gccctctgac ttctcgatct ccttgataat
     1981 atttttcaat ctctcttcga actcaccgcg gtattttgta cccgcaataa gaagcccgag
     2041 gtcaagcgac accaactctt tgtcacgaag agactctggt acattgccat tagccattct
     2101 gatagctaga ccctctacaa tagcagtctt acccacacct gcctcaccaa taaggattgg
     2161 gttgttcttg gtacgtcgag agagtatttg gatgatgcgc gagatctctt gatcgcgtcc
     2221 tatgacaggg tcgagtttgt ttagcttagc tagcttggtc agactcctcg tgtacttaat
     2281 aagggattta aatttcttct ctgtctttgg ctcggctgta ccagacttct tgagttcctc
     2341 gattattctt atcacagttg ctttgtctat cttaaacttg gtcaaaagtt ccttggcctc
     2401 acctggagat tctagtactg agaggaaaag atgctcggtc gaaacaaact cgtccccaag
     2461 tccttgagag atcttcgaag aagtgtcaat aatattggcc aactctggtg tgaggtagat
     2521 ctgatatgag ggtgagaggg tctgagagga ctctctactc tctattgctt cgagcacatt
     2581 gtcggtcaga aggatggagt cgatgtcgag cttgtcgata atagaaataa ccatactctc
     2641 ctcctgaagg atcagagctg ctaaaagatg aatgggatta acatggttct gtccacgctc
     2701 aatagcgagc tcgtgagctc ttcttatcgc ctctttggcc ttcgtcgtaa aatggttgaa
     2761 tggtggcata ttgttttgta atctctgtta cgagttatta gcaataaatt ccttgagcga
     2821 actgatcgat gtgtaaagcc cttgagttat cgataggtcc gtgtttgaag tctttagccc
     2881 gacgagctca ccggtcaaat taagtagtgg tcctcctgct gtctcgatgc cttttatatc
     2941 agtctctgca aacccggtag cgatattcag cgagaggatc ctacctacat taatagcgtt
     3001 tctctcgctt ccttctatag ctacaactgt ctgaccgagc tttggatctg attctgagag
     3061 agtaactgtc gagagagagc gaagcttgga atcatctgcg ataagtcgga agaggatcat
     3121 actacctgta tagctcacct tgtgtattgt tgccgagagt tcagacccgt cagagaagac
     3181 taccgtataa ttccctgtct ggataatgtc tcttctatca gagagcacaa gaccctgctt
     3241 attaaccaca acacctagcc cataaaaacg ctgaactcca tctgcaccat ttggagattt
     3301 tattcgaact acagccttag tgttgttctc aacagccgag actatcgcat caccctcttt
     3361 gacaatggtc acttccttaa ccgtacctcc tcctgatgga gtggtcactc tttcgacagt
     3421 gcgctcaact actcggtcaa tagtctgagt tactccttgt ggtgcctcgg ctaaaagaga
     3481 actcgtaata atcccggttg caatcgaagt tataaagctc accaaaagcg ttagtaggac
     3541 gatttgggtc tttgtaagat cttccatcgt tgtcataaaa cccattataa cacaacgtct
     3601 ttcaagattc taactacttt ttttatctca ctttctgtag tttttgggct gaaggaaaag
     3661 cgtatcgcat cagtatctcc atcgctcttt ctaaagaggc aggcactccc tgtagatact
     3721 gccacacctt taacatccag tttgataacc aagaactcat gtagggcacc cctcatactc
     3781 aaactcacaa tacttggaag actcccgacc ggagaattaa ttattgcttg gggtaccaaa
     3841 ctcttaactt cgcttaagaa taattctttc aatttggaaa ctttggcgaa gttcttctct
     3901 ctgtctctct cacactcttc gacaacctct acaaatttct gaattgcctc tacattctct
     3961 gtaccactcc ttagaccgaa ctcttgtcct ccgccaaaga taattggttt tatcaataca
     4021 ttacttcgaa cgataagtgc cgaaacgccc ttaggcccaa gaagtttaga tgtatcgaga
     4081 gtcactaagt cacatccaag tttggggaag tcaacagaca gatagcctag tgcctgagtg
     4141 gcgtcggtgt gtactagtgg gtacttggag ttgttcttgc ttcttatatc tcgaaccaac
     4201 cgtgcaatct ttggcaactc gcttacacca cccgtgtgac tgttcgcata gactattgag
     4261 atcaaaactg tattctcttt gatcgatttc aaaacggtat ttacattcaa taccccatct
     4321 tctaactcaa tgacagtgac ctccccacct cgtctctcta cctctttggc tgactcactc
     4381 accgcactgt gctctacact cgagataatg atgtgaggtt tatccatatt taccctcgca
     4441 gcctcaaaca cacccaaaat cgccaagttg tcggactctg taccaccgct tgtgaagacc
     4501 acctccttct tctgtacctt aaggagcctg gctagacgct cacgagagct ctctaatatc
     4561 cttttggccc cagctccctc ctcatgtatt gctgatgggt tatccctacc acccgcaaaa
     4621 tccaagaaaa ttctagctct ttttaatatt cccattgact cattctagaa ataaagcgtg
     4681 attttgcaac ttttttgtgc taaagtgccc ctactatgaa atctagaagg ccaccagtcg
     4741 tagcaataat gggacatatc gaccacggca agtcgactct gcttgattac atcaggaagt
     4801 cgaacactgt cgacaaagaa gcgggtggga tcactcagca tgtctctgca tatgaaatag
     4861 tacacacaga cggtgatggc ataatgaata agattacatt ccttgatact cctgggcacg
     4921 aggcattcaa ctctattcgt gcacgaggtg ccaaagcagc ggacattggt gttcttgtcg
     4981 tctcagcaga agatggagtc aaacctcaaa ctatagaagc tctaaaaaga attcgtgagg
     5041 caaatgtacc aatgatcgtc gctattacta agatcgacaa accttcggca aacatcgaga
     5101 tggcaaaaca atctctcgcg gagaacgacg tcttcgttga gggttacggc ggcgatgtgc
     5161 cagtggtggc attatccgct aaatctggcg ttggtataaa cgacttccta aacctagtgc
     5221 ttctcttggc tgaggtggag aactgcactg gtgaggaaga ccgtctaggc tctggagtga
     5281 tcatagaaag cagactcgat ccaaagaaag gtatcactgc agtaggggtg ctcaaggacg
     5341 gtactataaa gaccggacag gtagctgcgt ctataggcac tattgcacca ctaagattca
     5401 ttttggacgc aacggggacc aagatcgact cactcacctt ctcctctcca gtacagataa
     5461 ttggctggga ccgtctacct ctagtcggtg caacatttga aatatttgag aacaagccgg
     5521 ccgcacagtt ttatgccgac tctgaagcca tcaaggccca catacctaaa agcgagatgt
     5581 ctcaggcaaa tacagaagat ggctcgatat ctctacctat aatcattaaa gcagacgccg
     5641 caggaagtct tgaagctatc aaatatgaac taagcaaaat taataaagat aaaatttcta
     5701 taaaaatagt tagtgcagat gtcggtacta ttagcgaggg tgacgtaaag acgggcatga
     5761 ccacaccggg aacaataatc ttcggcttca acaccaagat cgatcctcaa gcaggtgcac
     5821 ttgcagagcg tacagagact atctcgatcg agctatacga cattatctac aagctgaccg
     5881 agagggtcga ggaacttcta aatgagaagg cacccaagtt tgaaacagag gaagtaatgg
     5941 gtagagcgaa agttctaaag tgctttagta agacaaagga taaacaagta ttaggtgccc
     6001 gaatggagtc aggactattc gagggtaaat ctaacttccg catcattagg agagaggagg
     6061 aagtcggccg aggtaaggtc aaagaacttc agcaatctaa agttgtggta gatagcatca
     6121 cagaaggtag tgagttcggt atgctcgtcg aatctaagac agagatcgct ccaggagacc
     6181 accttgagag agttgtaatg gttacgaaat aaaatgcgcc agatcaaaca cgataaagta
     6241 gaagagacta tacaccgcga ggctgctaag tttattttgg agaactctgg taactcgtct
     6301 ctcattacag tcacgcgtgt gactctcaat ccatctggca aagacgctat catctacttc
     6361 accacactgc ctgagagtga ggaagatact gctaaaaaat tcctagacag aaaagcacct
     6421 gagttcaagg atcacatgat gcatgagtct cgcattggca taatacccca cctccgcttc
     6481 caaatagact atggcgaaag gaaccgccaa aggttggatg agattggtaa ggatatacag
     6541 gcataaaagc tgtttttttg ataaggtagt gtatacggcc tagtcgccaa gtagtaaggc
     6601 aactctctgc aaaagagtta tacggcggtg cgattccgcc ctaggcctca atcggaaaca
     6661 agtttccgag tatgcccgag tggcggaatt ggtatacgcg cacgacttaa aatcgtgtct
     6721 cgcaagggat gtgggttcga cccccacctc gggcacaagc agataattct gcttttgtgt
     6781 ggtggtcgaa agccggagct atgtcgagcg agcacacgag attgcgaggc ggggtcgcga
     6841 ccaaggcgag tgacgacgag ccgagagttg tgaccgaccc ccacctcggg cacaatgcgg
     6901 gtatggttta gtggtagaat gcgaccttcc caaggttgag acaggagttc gattctcctt
     6961 acccgcacaa ggtgttaata tatatacatg agagaatcag gagaattccc acttatacca
     7021 gaaccagcac cacaagaaga gacacgtgct aaccgtctcg agtcttcaga tttaaatatc
     7081 agtgaagtgg aggattatat aaaaagcact aaatttgagg acaaagaaga gttggaggat
     7141 gttttattga aacagttcaa catgggcaag agagagagtt actcggatat ccagcttggt
     7201 atgacaagag ttataaaagc aattgggttc ggtaaagata ggaggatcat cgaagacaaa
     7261 ccattgtccg gcaaagagcc agaagcagac tcaattattg gtatagagac aatagacggt
     7321 aataagtatt atctgatgat atttaaggtc actaaccgta atcaaaagtt tagatacgaa
     7381 tacattctag gagcaaaata gtattgaaag tgtattaatt ttccgttata gtttcatttg
     7441 cctgcaccct tagctcagct ggtagagcag atccctctta aggataaggt cgaaggttcg
     7501 atcccttcag ggtgcacatt gggcgggtgg tgaaatggta gacacgcttg gcttaggacc
     7561 aagtgccgca aggtgtggag gttcaagtcc tctcccgccc aca
//
LOCUS       DAORIT010000022         7329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_1819_length_7329_cov_55.097333, whole genome shotgun sequence.
ACCESSION   DAORIT010000022 DAORIT010000000
VERSION     DAORIT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_7329_cov_55.097333"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="PLD99_02480"
     CDS             complement(<1..1429)
                     /locus_tag="PLD99_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRH25788.1"
                     /translation="MTRNSIARLAGFSAGAAMVLGFAAPVAAQSVAELQAQIAALMAQ
                     LSAMSGGASASSAITSDLTVGSTGSQVVALQNSLVSGGYLVMPAGVAPGYFGALTKTA
                     VAKWQAAVGLPATGYFGPLSRAKFTGSVAGTTTGGTTTGGTVSGTITTPGVEGTLTVT
                     SAPVSNSTLYEGENMVTVLGFKAEADLSDIAIQRVKIDLGTTNTLYRDILSKIYLVDD
                     AGKTLASADLNNSTVVRNGSNEYELTMTGFSSVVAKDATRNYMIKADVQDTIDSGDLT
                     SYTITLADNGVRGIDGAGIDLYSPALDTDVTKSVTFASSLTDSASLTVSANSSNPSSR
                     EIVAASGSSDNEADKVVLMVFDVRAEKDAIKVTDLQNFVITKSASATAGATASTSYLY
                     VGNGTGGQLLASAALTGSDADFTDIDYTIPAGTTRTFTLAVDVRSANAVQATFDASFT
                     GNTTNVIAENSLGDTINSVSGSADAN"
     gene            complement(1676..2317)
                     /locus_tag="PLD99_02485"
     CDS             complement(1676..2317)
                     /locus_tag="PLD99_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25789.1"
                     /translation="MNIEHKKTEKLISALYLLTSFFSDLEPMKWRLRDLGSKLLSVKE
                     SRSLVQEIMSLLTVSKNAGLISDMNHEIIHREFGNLLPKTESLGEMLKKIETHEEPVF
                     VAKEVLNKPEEKSVTNYLPRPNELQTLKDKIVRDVVKSEPVKDGAVALKKNGRQSAIL
                     NLLKKKPEIMIKDITPHIEGVSEKTIQRELLSLVKDGVLKKEGEKRWSRYSLA"
     gene            2499..4640
                     /locus_tag="PLD99_02490"
     CDS             2499..4640
                     /locus_tag="PLD99_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRH25790.1"
                     /translation="MAKKERKNRKEDVSGSFLSSLSEETRNAIFGVGSLVLTILLSMA
                     AFGVGGPVGGFVYKWLSFLFGVGYYLIPLVFLLLSISFFRAHEKNFALPQTIGSILFF
                     FSALGVVGVLLPTKGGIIGYWVAKPLLSLFAVYLSLIILIALIVVSLLVIFDTSIKVD
                     LMKWTKKLLMFKEKENTDTLLPVENVAIKEAVDKLGTNETDNKPEVEPVIKNFNTKKK
                     DEMDGFAPITLKSSGKAWTPPPLSLLERDKGKPGVGDIKANANIIKRTLQNFGIIVEM
                     DEISIGPSVTRYALKPAEGVKLSKILGLQTNLELALAAHPVRIEAPIPGKSLVGIEVP
                     NSAKTTIGLGSLLSEREYAESQKPLLVALGKDVAGESHFANIAKMPHALIAGATGSGK
                     SVTIHAIVTSLLYRNPPENMKFIMIDPKRVELTLYNKIPHLLTPVITDAKKAILALKW
                     ATKEMERRYEILEKESVRDIDSFHQSMKEGESMPFIVIIIDELADLMQAYPRELEAAI
                     VRLAQMSRAVGIHLLLSTQRPSVNIITGLIKANVPSRVALQVASQIDSRTILDAPGAE
                     KLLGAGDMLWLSGERGKPTRLQSAYISETEVKKVVKYLVDNYADELTEEINFSNENTS
                     NAIFSATLDSDPSDDDELYEEAREIVTQTGKASTSFLQRKLRIGYARAARLIDILEEK
                     GVVGPGSGAKPREVIGVSSDGGAEGFGQDEE"
     gene            4641..4964
                     /locus_tag="PLD99_02495"
     CDS             4641..4964
                     /locus_tag="PLD99_02495"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25791.1"
                     /translation="MKKILIIGFFVILGIYVVFQGRFIIFGPEISIELPKHGSIVDTG
                     PITVSGKVKNISYLSLNDGQIYTDNEGYFEEKLIAQDGTNIIKITAKDRFGRVREKLI
                     EIQAR"
     gene            5011..6003
                     /gene="recA"
                     /locus_tag="PLD99_02500"
     CDS             5011..6003
                     /gene="recA"
                     /locus_tag="PLD99_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRH25792.1"
                     /translation="MGERNESKNIDSTLDAIKMKFGEDSIMMLGAKPRVGIDSISTGS
                     IGLDAALGVGGLPRGRIIEIFGPESSGKTTLSLHVIAEAQKMNGVCAFIDAEHAMDPD
                     YAQRLGVKINELLISQPDTGEQALEIVESLVRSGKMDVIVIDSVAALTPKDEIEGEMG
                     AHHVGKQARLMSQALRKLTAIVAKSKTIVIFINQIRMQIGVMFGNPETTPGGKALKFY
                     TSVRIDIRRIAQIKKGEEIMGGRIRVKVVKNKVAPPFRQTEFDLMYNEGISREGEMIA
                     LGEKMGLLKKSGNSYEYGDVKLGRGYDATRQFLRENKPIGNQIMKEIRQKLKEE"
     gene            6016..6591
                     /locus_tag="PLD99_02505"
     CDS             6016..6591
                     /locus_tag="PLD99_02505"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRH25793.1"
                     /translation="MLEYNEITERKYIVFENEPYEVLSSHVFRKQQRKPVNATKMRNV
                     LTGRIVEHSFHVSDKATEADINKKEVKYLFTNKGEYWFCEPNNPSKRFELPAELIGDA
                     GKFLKTNITVDALLYDDGSEDGKIIGLKLPIKMELKVVEAHPAVKGNTAQGGTKAVVL
                     ETGAEIQVPMFINEGDIVRVNTETGQYTERV"
     gene            complement(6625..6816)
                     /gene="rpsR"
                     /locus_tag="PLD99_02510"
     CDS             complement(6625..6816)
                     /gene="rpsR"
                     /locus_tag="PLD99_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRH25794.1"
                     /translation="MKNPEVNYEKIDYKDADTLRKFLTPHARIQSRKRSGLPVSFQRS
                     VEVAIKRARFMGLLPYISR"
     gene            complement(6853..7302)
                     /gene="ssb"
                     /locus_tag="PLD99_02515"
     CDS             complement(6853..7302)
                     /gene="ssb"
                     /locus_tag="PLD99_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRH25795.1"
                     /translation="MYLNKAMVIGNLTRDPEMKSLPSGIQVTSFSLATNRVWKDKNGA
                     KQESVDYHNIVVFGRQAEIVSQFLRKGSSALIEGRMQTRSWDSPDGKKNYRTEIVADR
                     VQFGPRSSGAPASTSSPAKQDDDKSSIPPMETIDYPEESISLDDIPF"
BASE COUNT         2111 a         1638 c         1670 g         1910 t
ORIGIN      
        1 tgtttgcatc tgcagaacct gatactgagt tgatggtgtc accaagtgag ttctctgcaa
       61 taacatttgt ggtgtttcca gtgaaggaag catcgaatgt tgcttgaacc gcatttgctg
      121 agcgaacgtc aactgcgagt gtgaaggttc ttgtagtacc agctgggatg gtgtaatcga
      181 tgtcagtgaa atctgcatct gaaccagtca aagctgctga agcaagaagc tgtccacctg
      241 taccgttacc tacgtatagg taagaagtag aagcggttgc acctgctgtt gctgatgctg
      301 acttggtgat aacaaagttc tgaagatcag taaccttgat agcatccttc tcagctctta
      361 catcgaagac catgaggaca accttgtcag cctcattgtc gcttgaacca ctagctgcaa
      421 cgatctctcg tgaagatggg tttgagctgt ttgcagaaac agtgagtgat gcactatcag
      481 tcaagctaga tgcaaatgta actgacttag ttacatctgt atcaagagct ggagagtaga
      541 gatcaatacc tgctccgtcg atacctctaa caccgttatc cgctagggta atggtgtatg
      601 aagtaaggtc accagaatcg atggtgtcct gaacgtcagc cttgatcatg tagttgcgtg
      661 ttgcatcctt agccacaact gagctgaagc cagtcatagt aagttcgtac tcgttcgagc
      721 cgtttctaac aacagtactg ttgttaaggt cagctgaagc aagagtctta cctgcatcat
      781 caactaggta gatcttggaa agaatatctc tgtaaagagt gtttgttgtt ccaaggtcaa
      841 tcttaactct ctggatagcg atgtcagaaa ggtctgcctc tgccttgaag ccgagaacag
      901 taaccatatt ctcaccctcg tatagagttg agttagatac tggagctgag gtaactgtaa
      961 gagtaccttc aacacctggg gtggtgatag taccagaaac agttccacca gtggttgtac
     1021 caccagtagt tgtgcctgca actgaaccag tgaacttagc ccttgaaaga ggaccgaagt
     1081 aaccagtagc tggaagacca actgcagctt gccacttagc aactgctgtc tttgtaagag
     1141 caccgaagta acctggagct acacctgcag gcatcaccaa gtatccaccg gatactagtg
     1201 agttctgaag tgcaactacc tgtgagccag tagaaccgac tgtaaggtca gaagtgatgg
     1261 ctgaagatgc tgaagcaccg ccagacatag ctgaaagctg tgccataagc gcagcgattt
     1321 gtgcctggag ctcagcaact gattgtgcag ctacaggtgc ggcgaagccg agaaccatag
     1381 cagcacctgc tgagaaacca gcaagtcgtg cgattgaatt tcttgtcatt atttaattac
     1441 tatttctaat tgcgtttcgc tttcctgatt atttctaata atgttcaggt gacgaaacgt
     1501 ctgttaattg ccggcaacgg ttaagccttt gccggcaagc tcgtctaagt gagccaaact
     1561 tttgtttgca ctagttaatt atatagagct aaggcctttc gacccaaatc tatgtgtgga
     1621 taaccacagt taatcagcta gtaaaactgg gctcaattat acacattttg accctttaag
     1681 caagggagta acggctccat ctcttctccc cctccttctt caaaactcca tccttaacaa
     1741 gagagagtag ctctctttgt attgtcttct cactaacacc ctctatatga ggagtaatgt
     1801 cctttatcat gatctctggc ttcttcttca agagatttag tatggcagac tgtctaccat
     1861 tcttcttcaa agccacagcg ccgtccttta caggctctga cttaactaca tcacggacaa
     1921 tcttgtcctt aagagtttgc agttcgttag gtcttggcag atagttagtt acagactttt
     1981 cttctggctt gttaaggact tccttagcga caaaaactgg ttcctcgtgg gtctctattt
     2041 tcttaagcat ttcccctagt gactctgtct taggtaggag gttgccaaac tcacgatgaa
     2101 taatttcatg gttcatgtcg gaaattaggc cagcattctt agatacagta aggagactca
     2161 tgatttcttg cacaagagaa cgactctcct taacagagag aagcttggag ccaagatctc
     2221 ttagacgcca cttcattggc tcaagatcac taaaaaagct tgttaggaga taaagggcac
     2281 ttattagttt ttctgtcttt ttgtgttcta tattcatgtc ttttaactgt cttttactca
     2341 atgtccatta taacatgaaa tgtcctttat tgcaagcttt taagacataa gaagtatagg
     2401 acaagtaaga gacagatcgc aatagacatg ggcatctttt taatgcggaa agtgctctaa
     2461 aacgttgtga tataatgctt atatagaaag ctcgataaat ggccaaaaaa gagagaaaaa
     2521 accgaaagga ggacgtgagt gggtcgttcc taagctcact atctgaggag acgagaaacg
     2581 ctattttcgg cgtaggctcg cttgtcctaa ccattctcct atctatggca gcattcggcg
     2641 ttggggggcc tgtgggaggc ttcgtatata agtggctctc cttcctcttt ggggttggat
     2701 actatcttat acccttagta ttcctactcc tttcaatctc cttctttaga gcgcacgaga
     2761 agaactttgc cctacctcag acaattggct caatactatt tttcttctca gcacttggag
     2821 tggttggagt gcttttgcct actaagggtg ggatcatcgg gtactgggtt gcgaagcctc
     2881 tgctctcttt attcgcggtc tacctctccc tcatcatact tattgcactc atagtagtgt
     2941 ccttacttgt catttttgat acctcgatca aggttgacct aatgaagtgg acgaaaaaat
     3001 tactaatgtt taaagaaaag gaaaacaccg acacactact ccctgttgag aatgtagcta
     3061 ttaaagaagc tgtggacaaa ctaggaacaa atgagacaga taataaacca gaagttgaac
     3121 cagtgatcaa gaattttaat accaaaaaga aagatgaaat ggacggcttc gcaccaatta
     3181 cactcaagag ctcaggcaaa gcttggacac ctcccccact ctccttactt gaaagagaca
     3241 agggtaagcc aggggtcgga gacatcaaag caaacgctaa tattattaag aggacacttc
     3301 agaacttcgg catcatcgtc gagatggatg agatctctat cggtccatct gttactcggt
     3361 atgctctaaa accagccgaa ggtgtgaagc tctcgaaaat ccttggtctc cagacgaacc
     3421 ttgagctcgc tctcgcagcg cacccggtac gtatagaagc acccataccc ggcaagtctc
     3481 ttgtcggtat cgaagtaccg aacagtgcaa agaccactat cggacttggc tctcttcttt
     3541 ctgaaagaga gtacgcagag agtcagaagc ctttactagt tgccctcggg aaagacgttg
     3601 ctggagagtc tcacttcgca aacattgcca agatgcccca cgcgctaatc gccggtgcta
     3661 caggaagcgg taagtctgta acaatacacg ccatagttac ctctcttcta tacaggaatc
     3721 ccccagagaa tatgaaattc atcatgatcg acccaaagag agttgagctc actctttaca
     3781 acaagatccc ccatctcctc acccctgtta tcactgatgc taagaaggca atacttgcct
     3841 tgaagtgggc cacaaaagaa atggaaagaa gatatgaaat acttgaaaaa gagtcagtgc
     3901 gcgacatcga ctcattccac cagagtatga aagagggaga gtctatgccc ttcatcgtaa
     3961 ttattatcga cgagttggca gacctaatgc aggcatatcc tcgggaactt gaagctgcca
     4021 tcgtcagatt ggcccaaatg tctcgagctg tgggtataca cctcctcctc tcgacccaga
     4081 gaccttcggt caacattatt actggtctca ttaaggccaa cgtcccctct cgtgttgctc
     4141 tccaagtagc atcccaaata gactcacgca ctatccttga tgcgccaggt gctgagaaac
     4201 tcttaggcgc aggagacatg ctctggttat cgggagaacg tggcaaaccc actcgcctac
     4261 agtctgcata tatctcagaa actgaggtaa agaaggtggt gaaatacttg gtcgacaatt
     4321 atgcagatga actcacagag gaaattaatt ttagtaacga aaatacttcg aatgccatct
     4381 tctctgccac tctagactct gatccaagtg atgatgacga actatatgag gaggcgcgag
     4441 agatagtaac ccaaaccggc aaggcctcaa cctccttcct ccaaaggaaa ctccgtatcg
     4501 gttacgctcg tgctgctcgt cttatcgaca tcttggagga gaaaggggtt gtcggcccag
     4561 gctcaggcgc taaacccaga gaggttatag gagtatcatc tgacggcggt gcagagggct
     4621 tcggtcaaga cgaagaataa atgaaaaaga tactgataat aggcttcttt gtcattcttg
     4681 gaatatatgt tgtgttccag ggtcggttta tcatctttgg gcctgagatc agtatcgaat
     4741 tgcccaaaca tggcagtatt gtggatacgg ggcccataac agtaagtggt aaggtgaaaa
     4801 acatctcata cctctcccta aacgacgggc agatttatac cgacaatgaa ggctattttg
     4861 aggaaaaatt gatagctcaa gacggaacga atataattaa gatcactgcc aaagaccggt
     4921 ttggccgagt gagagagaag ctgatcgaaa tacaagccag ataaagaata aaaaatgaaa
     4981 aaaccaaaaa caccaaaacc gccaaaagaa atgggagaaa gaaatgaatc caaaaatatt
     5041 gactccacac ttgatgctat caagatgaaa ttcggtgagg actcgatcat gatgcttggt
     5101 gccaagccaa gagttgggat cgactctata tccactggat ccattggtct cgacgctgct
     5161 cttggcgtgg gcggactccc tcgtggacgc attatagaga tcttcggtcc agaatcttct
     5221 ggtaaaacca cactctctct ccatgttatc gctgaagcac aaaaaatgaa tggtgtttgt
     5281 gccttcattg atgccgaaca tgccatggat cccgattatg cacaaagact tggcgtaaag
     5341 atcaacgaac ttctcatctc tcaaccagac acaggcgaac aagcactaga aattgttgag
     5401 tcactcgttc gttcaggcaa gatggatgta atcgtcatcg actctgttgc ggctctaact
     5461 cctaaagacg aaattgaggg cgagatggga gcacaccatg tgggcaagca agcaaggctc
     5521 atgagccaag cactccgtaa gctcaccgct atcgttgcta agtcgaaaac tatagtgatc
     5581 ttcatcaacc agattcgtat gcagatagga gtgatgttcg gcaaccccga gactacccca
     5641 ggaggtaagg cgctcaaatt ctacacttca gtccgtattg atataagacg tattgcccag
     5701 attaagaagg gcgaggaaat aatgggaggt cgcataagag tgaaggtggt aaagaacaag
     5761 gtcgctcccc cattccgcca aactgagttc gacttaatgt acaacgaagg aatctctaga
     5821 gaaggagaaa tgattgcttt gggagaaaag atgggactac tcaagaaaag cggtaactca
     5881 tatgagtacg gtgatgtgaa gctcggtagg ggttacgacg ctacacgcca gttcctaaga
     5941 gaaaacaaac caatcggaaa ccagattatg aaagagatta gacaaaagtt gaaggaggag
     6001 tagaatgtca gtcctatgct tgaatataac gaaataacag agaggaaata catcgtgttt
     6061 gagaacgagc cttacgaagt actctcctca cacgtctttc gcaaacaaca aagaaagcct
     6121 gtgaatgcta ccaagatgag gaatgttctt accggacgca tcgtcgaaca ctcattccat
     6181 gtttcagaca aggctactga agcagacatt aacaaaaagg aggtcaaata cctttttacc
     6241 aacaagggtg agtactggtt ctgcgagcca aacaatccat ctaaaagatt cgaactccca
     6301 gctgaactta ttggcgacgc tggcaagttc cttaagacaa acataacagt agacgcccta
     6361 ctctacgatg atgggtcaga agacggcaag atcattggtc tcaagctacc catcaagatg
     6421 gaattgaagg ttgttgaggc tcaccctgct gttaagggta acactgccca gggaggcact
     6481 aaggcagtag tactagagac aggagccgag atccaagtac ccatgtttat aaacgaaggt
     6541 gatatagtca gagtgaatac cgaaactggt caatacactg aaagagttta atataaaaag
     6601 agcccctctc ggggctcttt ttgtttatct agatatgtaa ggaaggagtc ccatgaagcg
     6661 tgctcgcttg atggcaacct ctacagatct ctggaagctt actggcaaac cacttctctt
     6721 cctagactgg atacgagcgt gtggagtaag gaacttcctt agagtatcag cgtccttata
     6781 atctatcttt tcgtaattaa cttctgggtt tttcatttta atttattttt tactcgtagt
     6841 gagctcgtcg aactagaatg ggatgtcatc tagactaata ctctcctctg gatagtcgat
     6901 ggtctccatg ggtgggatac tactcttatc atcatcttgc ttggctggag atgaggtaga
     6961 agcaggcgca ccagacgatc ttggaccgaa ttgtacccta tcggccacaa tctcggttct
     7021 ataattcttc ttaccgtctg gactgtccca acttcttgtc tgcattctac cctcaatgag
     7081 agcagaagag cccttgcgaa ggaactgaga gacgatctcg gcctgcctgc cgaagaccac
     7141 aatgttgtga taatcgaccg actcttgctt ggcaccgttc ttatccttcc acactctgtt
     7201 tgtggcgagt gagaagctgg taacctggat acctgagggc agagacttca tctctgggtc
     7261 tcgggtcaag ttacctatga ccatggcttt gtttagatac atgactattg tggttggcta
     7321 ctagtagat
//
LOCUS       DAORIT010000023         7043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_1943_length_7043_cov_52.365341, whole genome shotgun sequence.
ACCESSION   DAORIT010000023 DAORIT010000000
VERSION     DAORIT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1943_length_7043_cov_52.365341"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLD99_02520"
     CDS             complement(<1..1028)
                     /locus_tag="PLD99_02520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRH25796.1"
                     /translation="MSSKSKRVLIFSISYYPLVGGAEVAVKEVTDRLTDIEFDMVTLR
                     FEKNHKSFEKIGNVNVYRIVGFKNLFPFLALFKAWRLNRERKYTHAWSIMANWAGFAG
                     FFFKLVNKETKFILTLQEGDSLSYIKRKVFFVYPIFKKIFTKADKIHAISNYLADWAR
                     DMGYKEKVEVIPNGVDYNKFESKEAKIKNDGKFVLITTSRLVKKNGVEDIIKAMSLLS
                     RDVSLHVLGSGPLDKQLRQKAKSLGVENRVQFIGFVDQREIPKFLHRADVFIRPSLTE
                     GMGNSFIEAMAAGLPVIATPVGGIVDFLTDPDKSRDKPPTGLFVPPRDPISIAKAVER
                     LIKNERLR"
     gene            complement(1018..2784)
                     /locus_tag="PLD99_02525"
     CDS             complement(1018..2784)
                     /locus_tag="PLD99_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HRH25797.1"
                     /translation="MYNPEKITYFAETDSRNKRVAFGIKAKDRLKHVYVIGKTGMGKS
                     TLLENMAVQDIKGNEGMAFIDPHGKTADLLLEYVPPERIKDVVYFAPFDTDFPISFNV
                     MEDVGPDKRHLVVNGLMSAFKKLFGEESFSDRMQYILQNTILALLEYPDSTMLGINRM
                     LTDKAYRDKVVANVTDSSVKTYWTQEFANYTERFAAEATPAIQNKIGQFTANPLVRNI
                     IGQKKSSFDFRKLMDEGKIMIINLSKGRVGEANANLIGSMLITKIYLAAMSRADTKES
                     DLNKLPNFYLYVDEFQSFANESFADILSEARKYKLNLTIAHQYIEQMEEEVRAAVFGN
                     VGTMITFRVGAYDAEVLEKEFAPVFTAEDLVNLGSYQIYLKLMIDGIGSQPFSASTLP
                     PIEKPEVSSVEEIIRHSRRVYAEPKAEVEKKIMDWHEEGKVAPLPKPPKGEKRNDFKK
                     EERRDERPREERRDFKPKQEFVRPEPVAQRPVSEQTQRPATPRVIVPQDDFKPLKSIT
                     LDTLKQKSNNQRPVSEKNVSDLRNALSSIIKTSNKVETQQLKQEPKIEVKVEPKVDLQ
                     PTETKKPKEIPEAELKALLDVE"
     gene            2867..3148
                     /locus_tag="PLD99_02530"
     CDS             2867..3148
                     /locus_tag="PLD99_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25798.1"
                     /translation="MFEEEDKFPILVNPKGLGRHNDLLGYIDITMTWRDFLLVRPEAE
                     QFVYALNPPAWTPLRAQPFQFIRNEDVKDLHERWSMQVDILLALKPTAQ"
     gene            complement(3161..4345)
                     /locus_tag="PLD99_02535"
     CDS             complement(3161..4345)
                     /locus_tag="PLD99_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HRH25799.1"
                     /translation="MLTRTPVHKHSNGFVWVGLLGILFLGITAFALPNYLKKDYTLAV
                     EDISLSKIVEEPKFKVTHIPTPEPMKAVYMSACVASTPSWRSDMKKFVEETELNSIII
                     DIKDYSGTISIYDESLQANDTTGCKVTDMMEFVGELHKSGIYVIGRVTVFQDPYYAKL
                     HPSLAVQSKSGGVWKDRKGLSFIDVGAKEYWDYIIDLSKKSYALGFDEINFDYIRYPS
                     DGNMADAHYSLTVGTSTKSEMLENFFSYLHENLKNTGVKTSADIFGYATLLPDDLGIG
                     QILEKVLPYFDFVYPMVYPSHYSQNTGGFGDPATHPYEIVKYSMESAVKREAAQRIVL
                     GVATSTPSKLKPWLQDFDLGANYGVAEVQAQIKATYDSGLTGWLMWDASNKYTRDAYL
                     KE"
     gene            complement(4311..4580)
                     /locus_tag="PLD99_02540"
     CDS             complement(4311..4580)
                     /locus_tag="PLD99_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25800.1"
                     /translation="MPKSKIIFFIGFFLGLQPIFLHFPKAWEDFFQVVGGLGIVLLSV
                     LISVDKRITRKAKAEKRQAVMRTKMAEEAQREFDNANQDTGAQTQ"
     gene            4631..4703
                     /locus_tag="PLD99_02545"
     tRNA            4631..4703
                     /locus_tag="PLD99_02545"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4664..4666,aa:Ile,seq:gat)
     gene            complement(4737..4871)
                     /locus_tag="PLD99_02550"
     CDS             complement(4737..4871)
                     /locus_tag="PLD99_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25801.1"
                     /translation="MADRPQGKFNAGLPEEEKSSKAPAFVVIVILGIVGAVLWAWLTP
                     "
     gene            complement(5331..6848)
                     /locus_tag="PLD99_02555"
     rRNA            complement(5331..6848)
                     /locus_tag="PLD99_02555"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1790 a         1764 c         1419 g         2070 t
ORIGIN      
        1 tctcttagcc tctcattctt gattagacgc tctacggcct tggctatgct tatagggtct
       61 ctaggaggca caaataggcc tgtaggaggc ttgtctcggc tcttatcggg gtctgtgagg
      121 aaatcgacta taccccctac tggagtggct attacaggca gtcccgctgc catggcctct
      181 ataaaggagt tccccatccc ctctgtaaga gagggtctga taaagacatc tgcccggtgc
      241 aagaacttgg gtatttctct ctgatctaca aagcctataa attggactcg gttctctacc
      301 ccaagactct tagccttctg ccttaactgc ttgtctaacg gcccagaacc aaggacatgg
      361 agactcacat ccctagacaa taaactcatc gccttgatga tgtcctctac accattcttc
      421 tttaccagtc ttgaggttgt aatgagaacg aactttccat cgttcttaat ctttgcttct
      481 ttagattcaa acttattgta atcaactcca tttgggatca cttccacttt ctctttataa
      541 cccatgtctc ttgcccagtc tgcaagataa tttgatatgg catgtatctt gtctgcttta
      601 gtaaatatct ttttgaaaat tgggtaaacg aagaaaactt ttctcttgat atacgaaagt
      661 gaatcaccct cctgaagagt aagaatgaat ttagtctctt tattaacaag tttgaagaag
      721 aatccggcga agccagccca gtttgccatt atgctccacg catgagtata cttcctctct
      781 ctattcaatc tccaagcttt gaagagtgct aagaatggaa agaggttttt gaatcctaca
      841 attctataga cattcacatt cccgatcttt tcgaaactct tatggttttt ctcgaacctg
      901 agagtaacca tatcaaactc aatatccgtg agtcggtctg tcacttcttt cactgccacc
      961 tctgctccac cgacgagtgg gtagtaagat atggaaaaaa ttaatactcg tttgctttta
     1021 ctcgacatct aagagggctt tgagttcagc ttctggaatt tcttttggtt tctttgtttc
     1081 tgttggttgc aaatcaactt ttggttctac cttaacttct atttttggtt cttgttttag
     1141 ttgttgagtc tctaccttat ttgaagtctt aatgattgaa gacagagcgt ttctcaaatc
     1201 actaacattc ttttccgaga caggtctctg attgtttgac ttttgcttaa gagtgtcgag
     1261 agtaatactc ttaagtggct tgaaatcatc ttgaggaact ataactcgtg gtgtggctgg
     1321 tctctgtgtt tgctcagaca caggtctttg cgccacaggt tctggtctta caaactcttg
     1381 ctttggctta aagtctcgtc tctcctctcg tggtcgttcg tctctcctct cttctttctt
     1441 aaaatcattc cttttttctc ccttaggtgg tttgggtaat ggagcaacct taccctcctc
     1501 atgccagtcc atgatcttct tctccacctc ggccttcggc tctgcataaa ctcttcttga
     1561 gtgacgaatg atttcctcta ctgaagatac ttctggcttc tctattggtg gcaaggtgct
     1621 cgcagaaaaa ggttgagaac ctatgccgtc tatcattaac tttaaataga tttggtacga
     1681 acccaggttc actaggtcct ctgcagtgaa tactggtgca aactcttttt ccaacacctc
     1741 ggcgtcatat gcacccactc taaaggtgat catggtacca acgttaccga agactgccgc
     1801 cctaacctcc tcctccatct gctcaatgta ctggtgcgca atagtgagat taagtttgta
     1861 cttcctcgct tcggatagaa tgtcagcaaa agattcattg gcaaaagatt gaaactcgtc
     1921 cacataaaga tagaagtttg gcagtttgtt caagtctgac tccttcgtgt ctgcacgact
     1981 catggctgca agataaatct tcgttatgag catagaacca atgagattgg cattagcctc
     2041 tcccactctt cctttagaga ggttgataat cataatcttt ccctcatcca taagttttct
     2101 aaagtcgaat gaggacttct tctgcccgat aatattcctc accaaggggt tcgcagtaaa
     2161 ctgcccaatt ttgttctgga tcgctggagt agcctcagcg gcaaatcgtt cagtgtagtt
     2221 ggcaaactct tgagtccaat aagtcttaac tgacgagtca gtgacatttg ctaccacttt
     2281 gtctctatac gctttatcgg ttagcatcct gttgataccg agcatggttg agtctggata
     2341 ctcaagcaga gccaaaatag tattttgcaa gatgtactgc atcctgtcgg agaaactttc
     2401 ttcaccgaaa agctttttga aggcactcat gagcccgttc accaccaagt gcctcttgtc
     2461 tggacccaca tcctccatca cgttaaaaga tattgggaag tcggtgtcga acggggcaaa
     2521 gtacaccaca tccttaatac gttcaggtgg cacatactca agaagtaagt ctgctgtctt
     2581 gccgtgtggg tcaatgaagg ccataccttc attacccttg atatcctgca cagccatgtt
     2641 ctcaagcaag gtcgacttac ccataccggt cttaccaata acataaacgt gcttaagtct
     2701 gtccttggcc ttgataccaa aagcgacgcg tttgttccga gagtcggtct cggcgaagta
     2761 tgtaatcttc tccggattgt acatttcctt atattataca acacaagctt gattttggct
     2821 tagataaagc ttaatcttgt cggagacctt attccggaga atatgaatgt tcgaagagga
     2881 agacaagttc cctatcttgg ttaaccccaa gggtttggga agacacaatg atctacttgg
     2941 ctacatcgat atcaccatga catggagaga cttccttctg gtgagacctg aagctgaaca
     3001 atttgtatat gctcttaatc caccagcatg gacaccgctt cgtgctcaac cgttccaatt
     3061 cattcggaac gaagatgtta aggatctgca cgagcgctgg tcaatgcagg ttgatattct
     3121 tctagctcta aagccaaccg ctcaataatg agcggttttt ctattccttc aaatacgcat
     3181 cccgagtgta cttattagac gcgtcccaca taagccatcc ggtaagtcca gaatcataag
     3241 ttgcttttat ttgcgcttgc acctctgcta ctccataatt cgcaccaagg tcaaagtctt
     3301 gtaaccaggg tttcaactta gacggagttg aggtggcaac accaagtaca attctctgag
     3361 cagcttctct ctttactgca ctttccatgg aatacttaac tatttcataa ggatgcgtcg
     3421 caggatcacc gaatccccct gtattctgag aataatgaga cgggtagacc atcgggtaaa
     3481 caaaatcgaa gtatggaagc accttctcaa gtatttgacc aatacctaag tcatcaggta
     3541 aaagcgtcgc atagccgaag atatctgcag aggtcttcac accggtattc ttcaaattct
     3601 catgtaaata agaaaagaaa ttctctaaca tctctgactt agtagaagtg ccgactgtta
     3661 gagagtaatg tgcatcggcc atattgccgt ccgatgggta ccgaatgtaa tcaaaattaa
     3721 tctcatcaaa tcctaatgca taactcttct tagaaagatc aatgatgtaa tcccaatact
     3781 ccttcgcacc gacatcgatg aatgacaaac ccttcctatc cttccacact ccgcctgact
     3841 ttgactggac tgcaagactt ggatgaagtt tagcgtaata agggtcttgg aaaactgtta
     3901 ccctaccaat tacatagata ccgctcttat gtaactctcc cacaaattcc atcatatctg
     3961 tcaccttaca gcctgtagta tcgttggctt gaagcgactc atcgtatata gatatagtcc
     4021 ccgaataatc cttgatatcg ataatgatgc tattcaactc tgtctcctcg acaaacttct
     4081 tcatatcact tctccatgaa ggagtgctcg caacacacgc ggacatatat acagccttca
     4141 tgggctcggg cgtgggtata tgagtaacct taaactttgg ctcttctact attttgctca
     4201 gagatatatc ttctacagca agcgtataat ccttcttcaa ataatttggg agagcgaatg
     4261 ctgtaatacc cagaaacaat atcccaagta acccaaccca aacaaaacca ttactgtgtt
     4321 tgtgcaccgg tgtcctggtt agcattatca aattccctct gcgcttcctc tgccatcttt
     4381 gttctcatca cagcttgcct cttctctgcc tttgctttac gagtaatgcg tttatcgaca
     4441 gaaataagga cagacagcag aacaataccc aaacctccaa ctacctggaa gaaatcctcc
     4501 caagctttag ggaagtgtaa gaaaattggt tgcaatccaa ggaagaaacc aatgaagaaa
     4561 atgattttac tttttggcat tttcaatatt atacacgaga gttttggtat gatatagttc
     4621 ttgccgaaat gcgcatatag ctcagctggt agagcacacg actgatactc gtgaggtcct
     4681 aggttcgatc cctagtgtgc gcacaaagat aaagaaaaag accgcaacga gtgcggtcag
     4741 ggagtgagcc atgcccagag aactgctccg acgatcccga ggataacgat cacaacgaag
     4801 gcgggagcct tcgagctctt ctcttcctcc ggcagtcctg cattgaactt gccctgtggt
     4861 cgatctgcca taactacctc actgaatggg tggtggcggg agcctaagta ctagattaag
     4921 gatcggcttg aggatgtcaa ccgaagaaaa tcttgtaaca cgtaaagccg atccaacctg
     4981 ctgcatagat gatacatccc cagatgaaca atttgccgag atccgtcaga atcttagcag
     5041 ttccttcaac ctgctcaggg cgtcgacctt gacgatacat ctcatcctca ttccggatca
     5101 caactcctcc attagagatt ctcgaaacag attactcttt atataaatgt agtcaacaca
     5161 aaaccgcccg aaggcggttt tgtctatcat tctctgtttt gctccgttca ccagtgcttt
     5221 cccggtgtca tgttgcattt tcggactttg caataattgt ccatcatacg ctcaattgag
     5281 gcgcacgata tcgactctgt aacctctcaa tgttattgag aagattttca aattgaatta
     5341 ctccacgagc agcttccgct acccgtgcct tgttacgact tactccctgt cgatgagctt
     5401 accgtgggca gacaaacgtc tgacttcggg tactcccatc tcccttgagt tgacgggcgg
     5461 tgagtacaag acttgagaac gtattcaccg cggtatagct gacccgcgat tactagtaat
     5521 tccggcttca tggggtcgag ttgcagcccc caatccgaac tggcgcacct tttcttagga
     5581 ttggctcagg gttaccccgt cgctacccgt tgtagatgcg attgtattat gcgtgtggcc
     5641 caaggtatca gagggacatg ctgacctggc gtcatcctcg ccttcctccc ccgtttgtat
     5701 aaacgaattt tgcaattcgc gcacacaaac gggggcagtc tcgccagaca cttgtaactg
     5761 gcaacgaggg ttgcgttcgt taccccactt aagggaacaa ttcaaatcac gaactgacga
     5821 cggccatgca tcacctgtct acgagtcctt gcggacggaa tctatttcta gaagccttca
     5881 cgtagatttc taaccttggt aaggtttttc gtttgtcatc gaattaaacc gcataatcca
     5941 ctacttgtgc aagtccccgt ctattccttt gagttttagc cttgcggccg tactacccag
     6001 gcgcttcact taacgcgtta gcttcgcctc cgagggggtc gattcccccg aaagctagtg
     6061 aagaacgttt agggcgtgga ctacctgggt atctaatcca gttcgctacc cacgctttcg
     6121 tgtttcagtg tcaggaatgc gccagtgaat tgccttcgct tttggtgttc cccatgatat
     6181 caacggattt cacccctact ccatgagttc cattcacccc tcgcatcctc tagtctggcc
     6241 gtttcccctg cagatcccgg gttaagcccg gggttttgac agaagactaa ccaaaccacc
     6301 tacacaccct ttacgcccaa taattccgga taacgcttgg ggcactcgta ttaccgctgc
     6361 tgctggcacg agtttagcaa ccccttattc ttcgagtact atcaaaactt tctcctcgac
     6421 aaaagatgtt tacgtcccag agggacttca tccatcacgc ggcgtcgctc cgtcagactt
     6481 tcgtccattg cggaagattc tcgactgcag ccacccgtag gtgtatgggc cgtgtctcag
     6541 tcccatcggt gggggtcagc ctctcagctc ccctagccgt catagccatg gtaggccatt
     6601 accctaccat ctagctgata gcccgcaggc cctttcctaa gcgtcttgcg actttgatcc
     6661 ggagatcaca tcgagtatta gccaatcttt cgactagtta ttcccgactt aagaggaggt
     6721 tcctacgtgt tactacctcg tctgccacta acacaaaatt gatgtaatcg ctttagtgtc
     6781 cgtttgactt gcatgcctta tccacgccgc cagcgttcat cctgagctag gatcaaactc
     6841 taaattgaaa tgaacggaac aaaaaagaaa acaatatgta tccttcttcg cccgactttc
     6901 agtttgtcct aactctactc acatctcgtg ttgctattag agatgtaagc ggttttattt
     6961 tagaaacatt tgagaacttg cttgtttctc aatcttaact tcaattttga aagaacaggc
     7021 tcggaccatt ccgagcgtgg ggt
//
LOCUS       DAORIT010000024         6290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_2313_length_6290_cov_53.172895, whole genome shotgun sequence.
ACCESSION   DAORIT010000024 DAORIT010000000
VERSION     DAORIT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2313_length_6290_cov_53.172895"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1787)
                     /gene="lepA"
                     /locus_tag="PLD99_02560"
     CDS             complement(9..1787)
                     /gene="lepA"
                     /locus_tag="PLD99_02560"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRH25802.1"
                     /translation="MERIRNFSIIAHIDHGKSTLADRMLELTNTIEKRKMKDQVLDSM
                     ELERERGITIKMQPVRMKFGEYVLNLIDTPGHIDFSYEVSRALKAVEGSILLVDATQG
                     VQAQTLTTLGMAKDAGLVIIPVISKIDSPLALIAEVKQEIMTLLGVDESEILMVSGKT
                     GEGVKELLNEIVKRVPAPKTDGVKDFRSLVFDFKYSNHKGVIVFVRAMSGIARKGDDL
                     VFQVADKKFQALEVGYFAPEETQAESLNAGETGYIVTGIKEPGIVSVGDTISLTTSVL
                     PPLDGYQKARPVVWASIYPESQDDFTLLKQALMKLRLSDSAFSFEEESSGVLGRGFRC
                     GFLGMLHLEIISERLRREFDLPLIITLPSITYIVTYKNGKTETIYTPSFFPEDHLIDN
                     VREPWVEAKIITPPKFLGDILKLLYEHEAEVGETQTLADRTSLSLQMPLRELMRGFFD
                     KLKSTTSGFASISYDILGERDADVTRLDILVADEAVPAFAKVISRRQVEGEAESAVEK
                     LSKILPRQMFTLKIQGQALGRIISSRTLSGMKKDVTQHMYGGDITRKMKLREKQKKGK
                     KKMAARGKGNVNIPQDVFLKMIRSDN"
     gene            complement(1801..3474)
                     /gene="murJ"
                     /locus_tag="PLD99_02565"
     CDS             complement(1801..3474)
                     /gene="murJ"
                     /locus_tag="PLD99_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRH25803.1"
                     /translation="MVRQIFSFFYKETHKLHQAAFLLAVFSLISQILGFVRDRSLAHY
                     FGASTNLDIYYAAFKVPDLIFVTFASVVSLSVLVPFIIERESEGRERVKKFVDDIFSF
                     FSFFILVSCLVFFWLMPEISRVLFKGFQPEALKEVVYLSRILLLSPIFLGVSNLFGSL
                     TQAYNRFLVYAFAPVLYNLGIIVGIFAFARSGEVSGVAWGVVLGSVLHLLIQIPFVIK
                     MELMPKLKIFEWVSIKRVIFHSLPRTVTLSLSQLSGVFLVALASLMTAGSISIFTFAQ
                     NISSVPLSIIGVSYSLAAFPTLSRHFSQNNIKDYLEQMSITVRHIIFWSMPIIALFVV
                     LRAQIVRVLLGTGNFDWADTRLTAASLAILAISALSQSLTLLFIRAFYSAGHTRKPFL
                     ISIFSTIFLALISYLFVKLFYFWPAFGLFISTLLRVEDLAGTVVLMLPLGFSVGMIVS
                     SIVYWRAFEKVFGKFGRETYHTLFVSLSTAVFVGLGSYIGLNLFEGWFNLETLLGIFG
                     QGFFAGILGILFGMFVLWLLKSVEFRELWQALHQKFGKVEAVAENQEIV"
     gene            complement(3497..5821)
                     /locus_tag="PLD99_02570"
     CDS             complement(3497..5821)
                     /locus_tag="PLD99_02570"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRH25804.1"
                     /translation="MQQDSKISFNVLLALVFLLPIFFMPTQVIPLGLAKISILVIASL
                     VCFAVLAYKMFKERSVAVVDTKLILVVLALPLIYIVSSLTSASPMFSMFGYALESTTA
                     GSILALSLLLLASAQIFRDRSSLIKVLTFFFASLVILAIFTVIKIVTGDSLLTLNTFY
                     GKMGTPIGAWTDLAVTIGLLSVMTIFVTEMLPLAKRIKFFTKILFLLSVVLLVIINFF
                     TAWMLVLCSALILMIYFGTVEKLEGVAYRKREGVRMAAVLLVLSVVFILNPSVNGKDL
                     TTKISEVTGVVNSDVRPNLKSTMIVAKTTLAKNPVLGSGPNTFDKEWLLSKPVETNAT
                     PFWNIAFPYGFGFIPTAVATTGILGTLAWAAFFIFYLMLGIKALSKSQENRSDKFIVI
                     STFFVSLFLWASAFTYAPSQTVIALAFIFTGLFIGALETTNVLNRKEYSFSQSRVKMF
                     IALLLLIVAVAGVAIFGFLSGKKVLAATHFQKALVYSNEGKTIDQIESELGKAISISP
                     ADPYWSAVSQVELRRANEALANTTATDEENQQTFQNALAASIAALQNAINLNPTYQNW
                     VALGNIYSSLVPKPFAVEGAYESAKVAYQSALSKHPLSPEVSLLMARLEVDNKNTKAA
                     REFIAEAISKKADYAEAYFFLAQLEIDQNNLDRAIESAETGTLLLPNNPGVLFQLGVM
                     KYSNSDYKGAVDSLQRALQLVPDYANAQYYLGLSLDKLGKKSEAIVQFENLAKTNPDN
                     AQVKDVLKNLKAGRAALSQAEEKEEKESAPIKGQ"
     gene            complement(5892..6195)
                     /gene="rnpB"
                     /locus_tag="PLD99_02575"
     ncRNA           complement(5892..6195)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLD99_02575"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1901 a         1480 c         1248 g         1661 t
ORIGIN      
        1 tcaataactt agttatctga cctaatcatc ttcaagaata catcttgagg tatgtttaca
       61 ttgcccttac cacgagcggc catcttcttc ttacccttct tctgcttctc tctcagcttc
      121 atcttacgag taatgtcgcc tccgtacatg tgctgagtca catccttctt cattcctgaa
      181 agtgttcggg aagatattat tcgtccgagt gcttgtcctt gaatctttag agtaaacatt
      241 tgtcttggca atattttcga gagcttctcc acggcgctct ctgcctcccc ctctacttgc
      301 ctcctcgaga taactttagc gaatgccggt accgcttcgt ccgccaccaa aatatcgagc
      361 cttgtcacat ctgcgtctct ctcacctaat atgtcgtatg agatagaagc aaagccggat
      421 gtagtgctct tcaacttatc gaagaagcct ctcataagct ccctaagcgg catttgtagg
      481 ctaagcgagg ttctgtctgc caaggtttga gtctcaccca cttcggcctc atgttcgtac
      541 aggagtttca aaatgtcgcc caaaaacttt ggcggagtga tgatcttagc ctcaacccaa
      601 ggctctctca cattatctat taagtgatcc tctgggaaga aagatggtgt atatattgtc
      661 tccgtcttac cattcttgta agtaacaatg tatgtaatag aaggcagtgt gatgataaga
      721 ggtaggtcaa actcacgcct taatctctca gaaataattt ctaggtggag catacccaag
      781 aacccacacc taaatcccct tcccaacact ccagaagact cctcctcgaa ggagaaggcc
      841 gagtccgata gccttagctt catcaaggcc tgcttgagta gagtaaaatc gtcttggctc
      901 tcgggatata tagaggccca gactactggg cgagcttttt gataaccatc taatggaggt
      961 aagacgctag tagtcaaaga tatggtatct cccaccgaca caatccccgg ctctttgata
     1021 cctgtcacta tgtaacccgt ctcgccagca ttgagtgact ctgcttgagt ctcctctggg
     1081 gcaaagtatc caacctcaag agcttggaac ttcttatctg ccacctgaaa gaccaagtcg
     1141 tcccccttcc tcgcgatccc actcatagct cggacgaaaa ctatcacccc cttatgatta
     1201 gaatatttga aatcaaagac caaagaccga aaatctttga ccccatcagt ttttggtgca
     1261 ggcactctct taactatttc gtttaagagc tctttcaccc cctcaccagt cttacccgag
     1321 accatcaaga tctctgattc atctactcct agaagggtca taatttcctg cttcacctca
     1381 gcgatgagag ctaggggcga gtcgatcttc gagatcacag gtatgatcac gagtccagca
     1441 tccttcgcca tacctaaggt cgtcagtgtc tgcgcctgga cgccttgagt agcatcgaca
     1501 aggagaattg agccctcaac cgccttaagt gctcgcgata cctcgtagga gaagtctatg
     1561 tgcccgggag tgtctataag gttaagtaca tactcgccaa acttcattct cactggctgc
     1621 atcttgatag taatacctcg ctcacgctcg agctccattg agtcgaggac ctggtccttc
     1681 atcttcctct tttctatagt attagtgagc tcaagcatcc tgtcagccag agtagactta
     1741 ccgtggtcta tatgagcaat gatagagaag ttacgtattc tttccatgag agtatgaacg
     1801 ctacacgatt tcttggttct cagcaaccgc ctcgactttg ccgaacttct gatgcagtgc
     1861 ttgccacaat tctctaaact caaccgactt aagtagccat agcacaaaca taccgaagag
     1921 aataccaagt attcctgcaa agaatccttg accaaagata ccaagcaagg tctctagatt
     1981 aaaccaacct tcaaaaagat ttaacccaat atacgaacct aatccaacaa aaaccgcagt
     2041 agaaagcgaa acgaaaagag tatggtatgt ctctctgcca aacttgccaa acaccttctc
     2101 aaaggctcgc caataaacga tactacttac aatcatcccc acagagaacc cgagtggcaa
     2161 catcaagaca acggtacccg caagatcctc aactctaagt aatgtactga taaataagcc
     2221 aaaagccggc cagaagtaaa acaacttaac aaacaagtag gaaattaagg ctaaaaatat
     2281 tgttgagaag atactaataa ggaacggttt cctggtgtgc cccgcagagt agaaggctct
     2341 tataaagagt agagtcaaac tctgcgacaa tgcagatata gccagtatcg caagtgacgc
     2401 agctgtaagt ctggtgtccg cccagtcaaa gttaccagta cccagcaaga ctcttacaat
     2461 ctgagctcgg agaacgacaa acagagcaat aataggcatc gaccagaata tgatgtgcct
     2521 aacggtgata gacatctgct cgaggtaatc cttaatatta ttttgagaaa agtgtctaga
     2581 caatgtggga aaggctgcca aagagtatga cactccgatg atagagagtg gtaccgaaga
     2641 aatgttttgt gcaaaggtaa atatggatat agaccccgct gtcataagcg aagccaaagc
     2701 taccaagaag actccagaga gctgactcaa ggacaatgtg actgttctag gtaatgagtg
     2761 gaaaattacc ctcttgatcg agacccactc aaaaatcttt agcttaggca tcaactccat
     2821 cttgatcaca aatgggatct gtattagtag atgaagcacg gatccgagca ccactcccca
     2881 ggccacacca ctaacttccc cactgcgagc aaaggcaaaa attccgacaa taatacctag
     2941 gttataaagt actggagcaa aggcatatac aagaaaccgg ttatatgcct gcgtaagaga
     3001 accaaacaag ttagagactc ccaaaaatat tggggaaaga agaagtattc tagacaaata
     3061 aactacctcc ttcaaagcct ctggctgaaa ccctttgaac agaactctcg aaatctctgg
     3121 catcaaccag aagaacacca gacaagaaac aagtatgaag aaagagaaga aagaaaatat
     3181 gtcatcaaca aatttcttta ccctctctct accctctgac tctctctcga taataaatgg
     3241 cacaaggacc gagagtgaca ccactgatgc aaaggtaaca aatattaggt ctggcacttt
     3301 aaaggcagcg tagtagatat cgaggttagt cgaagctcca aagtaatgcg ctaaggatct
     3361 atctctaaca aatcccaaaa tctgcgaaat taaagagaag acagcaagca ggaacgctgc
     3421 ttgatggagc ttgtgagtct ccttatagaa gaaactaaat atctgtcgaa ccatttactt
     3481 ttagtggttt gtcgaactac tgacccttaa taggggcgga ttccttttct tctttctcct
     3541 cagcttgaga caatgcagcc ctacccgcct tgagattctt caacacatct tttacctgag
     3601 cattatctgg attagtctta gcgagattct caaactgtac aattgcctca ctcttcttac
     3661 ccaacttgtc gagggatagt cctagataat actgtgcatt cgcataatct gggacaagct
     3721 gtagtgctct ttgtagcgaa tccactgctc ccttgtaatc actgtttgaa tacttcatca
     3781 ctccaagttg gaagagtacc ccggggttat ttggtaagag gagagtgccg gtttctgctg
     3841 attctatagc tctatccaaa ttattttgat caatctccaa ctgagcaagg aagaagtatg
     3901 cttcggcata gtctgccttc ttgcttatag cctcggctat aaactcgcga gcagccttgg
     3961 tgttcttatt gtctacttca agtcttgcca ttagaagaga aacctcgggg cttagtggat
     4021 gcttactcaa agctgattgg tatgccacct tagcgctctc atatgcaccc tcgacagcaa
     4081 aaggtttggg gactaaggaa ctatagatgt ttcctagggc aacccagttt tggtaagttg
     4141 gattaaggtt aattgcattt tgtagtgcag caatacttgc ggccaaagca ttctggaatg
     4201 tttgctgatt ctcctcatcg gttgctgttg tattcgcaag cgcttcattg gctcttcgta
     4261 gttcaacttg tgacacagca ctccaatatg gatcagctgg agaaatgcta attgctttac
     4321 caagttccga ttctatttga tctatagtct tcccttcgtt cgaataaaca agtgccttct
     4381 ggaagtgagt agcagctaac accttcttgc ctgacaagaa gccgaatatg gctacacccg
     4441 caacagcaac aatcaataaa agtagagcaa taaacatctt cactcggctt tgactaaatg
     4501 agtactcttt tctattcaac acatttgttg tctccaaagc tccgataaac aaacccgtaa
     4561 agatgaaggc caaggcaatc actgtttgtg aaggagcata agtaaatgca ctagcccaca
     4621 agaagagtga gacaaagaag gtcgagataa caataaattt gtccgatcta ttttcctgac
     4681 tctttgacaa agcctttata ccgagcatca agtagaagat aaagaatgca gcccaagcta
     4741 gagtaccgag gattccggta gtagctactg ctgtaggtat gaagccgaat ccgtatggga
     4801 aggctatgtt ccagaagggt gttgcattag tctcaactgg cttactaagc aaccactcct
     4861 tgtcgaaagt gtttggacca gagcctaaaa ccggattctt ggccaaagta gtcttagcaa
     4921 ctatcatggt cgacttcaag tttggtctta catcactgtt taccacccct gtaacttcag
     4981 aaattttagt agttagatcc ttaccattaa cactaggatt aagaataaac acaacagaaa
     5041 gtactaatag aacagccgcc attcttactc cttctctttt tctataagcg acaccctcta
     5101 atttctccac tgttccgaaa taaatcatga gtatcagagc agagcagagc accaacatcc
     5161 aagctgtaaa gaaattaatg attacaagta atactacaga caagaggaaa agaattttgg
     5221 tgaaaaactt aatccttttt gcgagaggca acatctcggt cacaaagatg gtcatcacag
     5281 acaagagtcc tatagttact gctaaatctg tccaagcacc tataggagta cccatcttgc
     5341 cgtagaaggt gtttagcgta agcaatgagt caccagtcac aatttttata actgtaaata
     5401 ttgcaagtat aacgagtgag gcaaagaaga atgtcagcac cttaataaga ctagaacgat
     5461 ctcggaagat ctgcgcagat gcaagcaaaa gaagagagag ggcaaggatc gatcctgctg
     5521 tagtgctctc aagagcatac ccaaacatag agaacattgg gcttgcactc gtgagagagg
     5581 agacaatata aatcagtggc aaagccagta ctactagtat cagtttcgta tccacaactg
     5641 ctaccgacct ctccttaaac attttgtagg caagaaccgc aaaacaaact aggcttgcta
     5701 taaccaagat cgagatcttt gccaaaccaa gcggtataac ctgagtaggc atgaagaaaa
     5761 taggtaaaag gaatactaga gccaaaagca cattgaaact gatcttgctg tcttgctgca
     5821 ttatttttaa tatttatttc taatgtgtgc atattttatc acaaacaaac acccaccaag
     5881 gttattggtg ggtgtttggg tgtctataag ccgaattctg tatttcgata aatcgaaacg
     5941 atagctattt atctggtcta ggtatcacta cctagatcta gcggcactcc ccataggggc
     6001 acggccttgc attcaagtaa gaattttgcc gtttcagccg gacttaaccg gatcgtttct
     6061 gttcgcatct ctaggattac tcccggcagg agttacctgc tactcttgtt gtgtatgagc
     6121 atatgcagac acacacaact gaatgttcgg actttcctcc ccacccgaag gcagagcaac
     6181 tatccgacac ctctattatt ataccacaat taagcttaat ttcaaatgtt aataacaaca
     6241 cggattatat ccttatgtct atgatacaat tttgaaatga tttccaaaaa
//
LOCUS       DAORIT010000025         6285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_2315_length_6285_cov_54.880899, whole genome shotgun sequence.
ACCESSION   DAORIT010000025 DAORIT010000000
VERSION     DAORIT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_6285_cov_54.880899"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..340)
                     /locus_tag="PLD99_02580"
     CDS             complement(89..340)
                     /locus_tag="PLD99_02580"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRH25805.1"
                     /translation="MNFKDAVYAVVRKIPRGEVMTYKEVAKKAGKPLAFRAVGNILNK
                     NTEKGVPCHRVIRSDGKIGGYNGLQGQKRMLLVKEGYLK"
     gene            complement(370..720)
                     /locus_tag="PLD99_02585"
     CDS             complement(370..720)
                     /locus_tag="PLD99_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25806.1"
                     /translation="MPAKKRAGIIGEERWYIHSDSKGFKVLFGHVSFEEHGQMARMCT
                     DGRRRDFSEFGDKEETLRLAKDLRDQGFKAEVFFNFGNGIRPYVHEVKPKETRVSLSD
                     LIKRILGMTPATSK"
     gene            811..882
                     /locus_tag="PLD99_02590"
     tRNA            811..882
                     /locus_tag="PLD99_02590"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:844..846,aa:Glu,seq:ctc)
     gene            906..1241
                     /locus_tag="PLD99_02595"
     CDS             906..1241
                     /locus_tag="PLD99_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25807.1"
                     /translation="MKKISIIIILISIFAVGYMWWPGLMSQYHSLNDNTPQGKLMSVE
                     SYVSQNISELSPIKEVLGGKFYVTDIEVLDGRGTVSYEDGHIAYTADFSYTSSDRTGH
                     NITSFVIRN"
     gene            complement(1242..2543)
                     /gene="murF"
                     /locus_tag="PLD99_02600"
     CDS             complement(1242..2543)
                     /gene="murF"
                     /locus_tag="PLD99_02600"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRH25808.1"
                     /translation="MKEILKQILVVVLHKLSHLVIKKYKPKIIGITGTVGKTTTKDAV
                     YTALSRFESVRKSQKSFNSELGIPLTILDSDNPGRDMLGWLRVIVDGLVMLFFKSPYP
                     KWLVLEVGTDKPGDIAMITKWLKTDITIVTKLSKVPVHVEAFGAPEYLFEEKGNLVKA
                     LKPGGTLILNADDLDVMAYKSLCEEKILLFGEKLGSDIRSSDYQILYDEQKLPHGIEF
                     NVLIGDTESHRITLADTLGEQNISHVLVALLVVKDLGLDVRLAASSFKSEHPTPGRMR
                     LLDGLKGSLLIDDSYNSSPVALEEALKTLKSIKIGRGHRKIAILGDMLELGRYTVDEH
                     KKMGERASKSATILVTVGIRSRYAAEAALSGGMSESKVFQFDDSLEAGMFMKDLIKSG
                     DVILIKGSQGTRMEKCVEELMAKPEYKEKLLVRQSTDWADK"
     gene            complement(2545..4293)
                     /locus_tag="PLD99_02605"
     CDS             complement(2545..4293)
                     /locus_tag="PLD99_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRH25809.1"
                     /translation="MHQGRLRFLSFLTIGFALILIGKLYTVQVVNTETYRAKADHQYV
                     EGTNYFDRGSIFFTTKSGDLVPGASVKSGYILAINPQLFRLQGVNPDSVFEKLSKVYE
                     IEKSFWDSKASRDYDPYEELAKKLPRESAEMIRDLNIPGLDVYKEDWRIYPGGEMSAH
                     ALGFMGFGGESGNEYKGRYGLERSYEEVLVRSGDKAFVNFFAEVFSDIKKSITPGVSP
                     EGNIITTIEPTVQAMLEEELGNIQEKYNSEFTGGIIVDPKTGAIIGMALNPTFDPNTP
                     SSASSVGVFSNKLVEDRYEMGSILKALTMAAGLDSGAVTRKTTFNDPGCKTLDKKTFC
                     NFDKKSYGTKVPMQLVLSKSLNTGAAFVVEKMGNSKFRDYMLSYGLASTTGIDLPNEG
                     KSLVANLSVPRDLEYAQASFGQGVAYTPIQMVRALSVLANGGYLTTPHLVRKIDYELG
                     ITKDVTPPKGKQIFKPGTTEEISRMLTEVVDTALRDGKVRLDKYSVAAKTGTAQIANP
                     SGGGYYADHYLHSFFGYFPSYDAKFLVFLYTFHPQGVQYASETLTDSFINLTKFLINY
                     YNIPPDREMPPKATLD"
     gene            complement(4303..4578)
                     /locus_tag="PLD99_02610"
     CDS             complement(4303..4578)
                     /locus_tag="PLD99_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25810.1"
                     /translation="MFWIFSITICLSLGVYGYAVLATINHTIAKKNIVESSSTLATRV
                     SELEFAMIGEKNDVDLETALSYGFKEVRNPIYISRISNSLTLNTEGR"
     gene            complement(4607..5515)
                     /gene="rsmH"
                     /locus_tag="PLD99_02615"
     CDS             complement(4607..5515)
                     /gene="rsmH"
                     /locus_tag="PLD99_02615"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRH25811.1"
                     /translation="MKHVPVLLHEIIDGLNLKDGDIFLDGTLGSAGHTLAVAERFGDS
                     VEIIGIDRDEEALRRSEERLRTFNHSAYLKLSSFRNLDDVLRDLGVREVNAILLDLGI
                     SSEQIDDSGRGFTFKKDEPLSMSMEKDPKGVTAKEILNHWDEETLEIILRGFGEERFS
                     KRIAKAIVDRREIKPFETTFDLLEAVDAALPASLKNRGYQKINPATKTFQALRIAVNE
                     ELASLEEGLTKAFNALGDKGRLAVISFHSLEDRIVKNFMRDLAKSGQARLITKKPITP
                     SDEEVDANPRSRSAKLRIIEKNAIQN"
     gene            complement(5517..5951)
                     /gene="mraZ"
                     /locus_tag="PLD99_02620"
     CDS             complement(5517..5951)
                     /gene="mraZ"
                     /locus_tag="PLD99_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRH25812.1"
                     /translation="MFIGEYLHTVDEKKRISLPAKFRKELGQKVVLTRGLDNCLFLFP
                     HKTWKAISTEMAKLGMMQADTRGFSRFMFSGASEVEVDSLGRVLIPEFLKEFAGIKNP
                     VIFAGVHDRIEIWNDKRWQDYKKRIEADAEGMAEKLGSVGLK"
BASE COUNT         1655 a         1585 c         1223 g         1822 t
ORIGIN      
        1 gagccagctc ccgcgtggag acaggaacga tgatgattga gttacaaagg gcggactcca
       61 gcacattgga gtccgccctt attcgtgtct attttaaata cccttctttt accaacaaca
      121 ttcgcttctg tccctgtagt ccgttgtatc caccaatctt tccatctgac ctaatcacac
      181 ggtgacacgg cacacctttc tcagtgttct tgtttagaat atttcctact gcgcgaaagg
      241 ctaatggctt cccagccttc tttgccactt ctttgtaggt catcacctcg cctctcggta
      301 tttttctcac cacagcataa acagcatctt tgaaattcat gtgagtatta taaaagaaaa
      361 agccacgagt tacttactcg tggcgggtgt catacccaga atcctcttga tcaagtcact
      421 gagactcacc ctcgtctcct tcggcttgac ctcatgcaca taaggcctaa tgccattccc
      481 gaagttgaag aagacctcag ccttgaaccc ctgatccctc aggtccttcg ccaagcggag
      541 tgtttcctcc ttatcgccaa actctgagaa atccctacga cgcccatcag tgcacattcg
      601 tgccatctgc ccatgctcct cgaaggaaac atgtccaaac agaaccttga accccttgga
      661 gtctgagtgg atgtaccacc gctcttcgcc aatgatccca gcccttttct ttgcaggcat
      721 tctgcctcct atatacggtt ctttgcattt tataacacat aaattaaact ttgaaaaata
      781 tctggacttt tgctattctt ctctagctac ggccctatcg tctatcggct aggacacatc
      841 cttctcaagg atggaagcag ggttcgactc cctgtagggt cacaaattta ggttagaatg
      901 aaggtatgaa aaaaatatca attatcatca tcttgatatc aatttttgca gttggttaca
      961 tgtggtggcc aggcttgatg agtcaatatc attctttaaa cgacaatact ccccagggta
     1021 agcttatgtc agttgagagc tatgtatcgc agaatatctc cgagctatcg cccatcaaag
     1081 aagttttggg tggcaaattc tacgtgactg acattgaagt acttgatggc agaggcacag
     1141 tgtcgtacga agacggacac attgcttata cagccgactt ctcttacaca tcttcagacc
     1201 gcacaggtca caacatcacc tcgtttgtaa ttcgtaacta attatttatc agcccagtcg
     1261 gttgattgtc tgaccaaaag cttttctttg tactcgggct tggccataag ctcctctacg
     1321 cacttctcca tcctggttcc ttgagaaccc ttgatgagga tgacatctcc ggatttgatc
     1381 aaatctttca taaacatacc agcctctagt gagtcatcaa attgaaacac cttgctctcg
     1441 ctcataccac cactcaatgc tgcctctgcc gcatatcgag agcgaatacc aactgttact
     1501 aaaattgttg ctgacttaga agctctttca cccatcttct tatgctcgtc gacagtatag
     1561 cgaccaagct cgagcatgtc gcctagaatt gcgatctttc tgtgacctcg acctatctta
     1621 atcgacttca aagtcttgag tgcttcttct agtgctactg gagaggagtt atatgagtca
     1681 tcgatcaaga gcgatccctt aagaccatcg agtaacctca tcctacctgg cgtagggtgc
     1741 tctgacttaa atgagctggc agccaacctt acatctaagc caagatcctt aacaacaagt
     1801 aatgccacta aaacatgaga aatattttgc tcgcctagag tatctgcaag tgtaatcctg
     1861 tgtgactcgg tatcccctat caatacatta aattctatac cgtgaggaag cttctgctca
     1921 tcataaagaa tctgataatc actagagcga atatcgctac ccaacttctc accaaaaaga
     1981 agtatcttct cctcacacaa actcttgtac gccattacat ccaaatcatc tgcattgagt
     2041 ataagtgtgc cacctggttt aagtgccttc accagattgc ccttctcctc aaagagatac
     2101 tcaggagcac caaaagcctc aacatgcaca ggtaccttgg acagctttgt aacgatagtt
     2161 atatcggttt tgagccactt ggtaatcatg gcaatatcac ctggtttgtc ggtaccaacc
     2221 tctaacacca gccactttgg gtacggagac ttgaaaaaaa gcatcaccag tccatcaact
     2281 attaccctta accaacccaa catgtctcgt cctgggttat cgctgtctaa gatggttaac
     2341 gggataccaa gctcactgtt aaaacttttt tgactcttcc tgacactttc aaacctagaa
     2401 agtgcagtat agacagcatc tttggtggtg gtcttaccta cagtaccagt aataccaatt
     2461 atctttggtt tgtatttctt tataacaaga tgagatagtt tgtgcagcac aacaaccaga
     2521 atctgcttga ggatttcttt cattttagtc tagggtagct ttaggtggca tctctctgtc
     2581 tggcgggata ttgtaatagt ttatcagaaa tttagtgagg tttataaagc tatctgtaag
     2641 agtctcagat gcatattgca caccttgtgg gtggaaggtg tagaggaata ctagaaattt
     2701 tgcgtcatat gatgggaagt atccaaagaa tgaatgtaag tagtggtcag catagtatcc
     2761 accgccgcta gggttcgcaa tttgtgcggt accagtcttt gccgctacag aatatttgtc
     2821 gagcctaacc ttgccatcac gaagagcggt gtcgaccacc tcagtaagca ttctagagat
     2881 ttcttctgta gtgccgggct tgaatatttg tttgccttta ggtggagtaa catcttttgt
     2941 aataccaagc tcataatcta tcttccttac caaatgtgga gtagtgagat acccaccatt
     3001 agctaatacc gaaagagccc gaaccatctg tatgggtgta tacgccactc cctgaccgaa
     3061 tgaggcctga gcatactcta gatctcttgg aacactaagg ttagcaacaa gcgatttacc
     3121 ctcattaggt aaatcaatac cagtagtaga tgctaagcca tatgagagca tatagtctct
     3181 aaacttgctg ttacccattt tctccactac gaacgcagcc cccgtgttca gtgacttgct
     3241 caaaactaac tgcataggga ctttcgtgcc atatgacttc ttgtcgaaat tacaaaaggt
     3301 cttcttgtcc aaagtcttgc acccaggatc gttgaaggtc gtcttcctag taactgcacc
     3361 tgagtcaagt cctgctgcca tagtgagagc cttcaagata gatcccatct catacctatc
     3421 ttcaacaagt ttatttgaaa atactccaac cgatgaggca cttgaaggag tgttcggatc
     3481 gaaggttggg ttaagtgcca taccaataat ggccccagtc tttggatcaa ctattatccc
     3541 tccagtaaac tcagaattat atttttcttg aatgttaccg agctcttcct caagcatagc
     3601 ttgaactgtt ggctcgatag ttgtaatgat attaccctca ggactaacac cgggtgtgat
     3661 ggacttcttg atatcagaga aaacctcggc aaaaaagttc acaaaggctt tatctccact
     3721 ccttaccaac acctcctcat acgacctctc tagaccatat ctacctttat actcgttgcc
     3781 gctctcgcct ccgaaaccca taaagccaag tgcgtgagca gacatctcac cccctgggta
     3841 gatcctccag tcctccttat agacatcaag gcctgggata ttaaggtcgc gaatcatttc
     3901 tgctgactct ctggggagct tcttcgctag ctcttcgtat gggtcgtagt ctctcgaagc
     3961 cttggaatcc caaaaagatt tctctatttc ataaactttg cttaacttct cgaacaccga
     4021 atccggatta actccttgta gccgaaaaag ttgtgggtta atagccagga tgtaaccact
     4081 tttgaccgat gctcccggta ctagatctcc actcttggta gtgaaaaaga ttgaccctcg
     4141 gtcaaaataa ttggtgccct ctacatactg gtggtctgcc ttagctctgt aggtctcagt
     4201 attaactacc tgaacagtat aaagtttgcc tataagtatc aaagcgaaac cgatagttag
     4261 aaaagaaaga aatctaagtc gcccttggtg catacctaca ttctatcgtc cttctgtatt
     4321 aagtgtaagc gaattactga ttcgagagat ataaattgga ttacgaacct ctttgaaacc
     4381 gtacgagagt gcagtctcga gatcaacatc attcttctcg ccaatcatag cgaactcaag
     4441 ctcactcaca cgagttgcca aggtgctact agactcaacg atattcttct tagcaatagt
     4501 gtgattaata gtggcaagta ccgcatagcc atacacccct agtgacaaac aaatagtaat
     4561 actaaatatc caaaacattc ttactcgtgt atctattgaa attgttttag ttttggattg
     4621 catttttttc gataattctt agtttggcgc ttctagatct tgggttcgca tccacctcct
     4681 cgtcactcgg ggtaatgggc tttttagtga ttaacctggc ttgacctgat ttagctaggt
     4741 cgcgcataaa gttcttcact atcctgtcct caaggctgtg gaaggaaatg acagcgagcc
     4801 tgcccttgtc tcccaaagcg ttgaaagcct tggtcagacc ctcttctaga gaggcgagtt
     4861 cttcattaac agcaatacgg agggcttgaa aggtcttcgt tgctggattg atcttttgat
     4921 aaccacgatt cttgagagag gcaggtaggg cagcatctac cgcctcaagg aggtcgaagg
     4981 tggtctcaaa cggtttgatc tctcttctat cgacaatggc cttggcaatg cgcttggaga
     5041 atctttcttc tccgaagcct cggagaatta tctcgagagt ctcttcgtcc cagtggttga
     5101 ggatttcctt ggcagtaact ccctttgggt ccttctccat cgacatactg agtggttcat
     5161 ccttcttgaa ggtgaatccc ctacccgagt cgtcaatctg ctcactggat attcctaggt
     5221 caagtaagat tgcattaacc tctctcacac ccaagtcacg aagtacatca tccaagtttc
     5281 taaagctcga aagttttaaa tatgctgagt gattaaaagt ccgcaacctt tcctcactgc
     5341 gtcttagagc ttcttcgtct ctgtcgatcc caatgatttc gacactgtct ccgaacctct
     5401 cggctaccgc tagagtgtgt ccagcactac cgagtgtccc gtcgagaaaa atatctccat
     5461 ctttgagatt taaaccgtct atgatctcgt gtaaaagtac aggtacgtgc ttcatgttat
     5521 ttcagtccga cactgccgag tttctcggcc atgccttctg catctgcttc gatcctcttc
     5581 ttgtagtctt gccagcgctt gtcgttccag atctcgatac gatcatgaac acctgcgaaa
     5641 ataactggat tctttatccc agcgaattcc ttcaagaact cggggataag aactctaccc
     5701 aaagaatcta cttcaacctc agatgcacct gagaacatga acctcgagaa tccgcgggta
     5761 tcagcctgca tcatcccgag cttagccatc tcagtcgaga tcgccttcca cgtcttgtga
     5821 ggaaagagga aaagacaatt atccaagcct ctggtgagta ccactttctg accaagttcc
     5881 tttcgaaatt tggctggtaa cgagattcgt ttcttctcgt cgactgtgtg tagatactcg
     5941 cctataaaca ttgcttgttt ttattatcat accactttat cccacctaca caccattgta
     6001 tcccaccact ctccacatac ctactattct tatccacaac cactcacttt agtaagaaaa
     6061 aaaggcgcaa gttccgtaga acctgcgccc ttgcactaaa gacgagtcta gaactcgatc
     6121 taccccttct tccagaccac ttcgccagtc ttcctgtcga agaggccctg cttcgcaccc
     6181 ttccgcacca agtagaaggt gccggagaga tgggagatat cgtcgtactc atggttgatc
     6241 acgaatttct tcaacttgat gtcgaagatg tcatacttcc cgccc
//
LOCUS       DAORIT010000026         3736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_5449_length_3736_cov_49.281445, whole genome shotgun sequence.
ACCESSION   DAORIT010000026 DAORIT010000000
VERSION     DAORIT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5449_length_3736_cov_49.281445"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..273)
                     /locus_tag="PLD99_02625"
     CDS             complement(133..273)
                     /locus_tag="PLD99_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25813.1"
                     /translation="MIQKARQSGGLELISLLFAEPWFLDEEYHEHERTETEEHKIVQE
                     LG"
     gene            303..1226
                     /locus_tag="PLD99_02630"
     CDS             303..1226
                     /locus_tag="PLD99_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRH25814.1"
                     /translation="MYDLIIIGGGPAGISAGVYAARKRLKTLIVTESFGGQSVDSMGI
                     ENWIGTQKISGPDLAKQLESHLRSYESDSVVIKTGVRVSKVEKIDNIFSVTMGEETAK
                     ATTVLITSGSSRRKLDIPGGKEFENKGITYCASCDGPLFADMDTVVIGGGNAGFETAA
                     QLLSYAKSVTLLHRADKYRADEMTVEKVLGNPKMKGILNALPIEIKGDKFVKSVVYTD
                     KMTGENVEIPASGVFVEIGLVPNTSFVEGVVPMNEFKQIIADPRTGRTETLGIWAAGD
                     VTDTMYHQNNIASGDAVRALENIYMYIRTGR"
     gene            complement(1953..2039)
                     /locus_tag="PLD99_02635"
     tRNA            complement(1953..2039)
                     /locus_tag="PLD99_02635"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2003..2005),aa:Ser,seq:cga)
     gene            2093..2164
                     /locus_tag="PLD99_02640"
     tRNA            2093..2164
                     /locus_tag="PLD99_02640"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2126..2128,aa:Glu,seq:ttc)
     gene            2169..2241
                     /locus_tag="PLD99_02645"
     tRNA            2169..2241
                     /locus_tag="PLD99_02645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2202..2204,aa:Val,seq:gac)
     gene            2244..3296
                     /gene="tsaD"
                     /locus_tag="PLD99_02650"
     CDS             2244..3296
                     /gene="tsaD"
                     /locus_tag="PLD99_02650"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRH25815.1"
                     /translation="MKILAIETSCDETAICLLECSGSLPSPTFKVIANSLISQIEIHK
                     EFGGVFPMLAKREHTKNLPIILDKVLKDAGEDPNKPDIDYIAVTSGPGLEPALWTGIV
                     FAEELSQKWGKKVIPINHMEGHLFSSIFQNTDTLELPAIALLVSGGHTELVLVESFDK
                     FRIIGRTRDDAVGEAFDKVARLLDLPYPGGPKISELANRSRARSKEGTFTFPRPMMKT
                     SDYDFSYSGLKTSVLYAIQKLDSINNETKEDIARGFEEAAIEPLVYKTKSAIEEFGVG
                     SIILGGGVSANTYLQSELKTLSNEYGLPLHLPKSDLSTDNAIMIAVAAYIRIVTERIS
                     IPEKIVANGNLSLDSN"
     gene            complement(3293..>3736)
                     /locus_tag="PLD99_02655"
     CDS             complement(3293..>3736)
                     /locus_tag="PLD99_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25816.1"
                     /translation="QLEEDKGQFSQFAAAVFSFPETLRDKMQCLYELALILFVLYILG
                     SIVSDVLYKKGVEKSFKKFAYKWTTIVLGLTIAIILAFLLKEFCLLLPLIIALVLSLL
                     WIAMYPKHDTVRTKLSSFKKKTHVTETTIDLPGAFEIKEDDKLDF"
BASE COUNT         1039 a          857 c          939 g          901 t
ORIGIN      
        1 cttatccacg gttttacgac aagccctccc cttgtcggga taatggataa atatgagggt
       61 atgtactatg atggccaaag ttcttaacca ggagaccggg tgactgaaaa aaggaagaag
      121 gaacctagcg ggttatccaa gttcttgtac gatcttatgc tcctctgtct cggtgcgttc
      181 atgctcgtgg tactcctcat caaggaacca tggttcggca aaaaggagag aaattagttc
      241 tagtccgcca ctctggcggg ctttttgtat catgaaaaaa tttctgatat ccttattcca
      301 ctatgtacga tttgatcatc attggaggag gcccagcggg tatatcggcg ggtgtgtatg
      361 cagcgaggaa gagacttaag actctcattg ttaccgagag cttcggcggt caatcggtag
      421 actcaatggg catagagaat tggatcggta cccaaaagat ttctggtcca gacttggcca
      481 agcaactcga aagtcatcta agaagttacg agagtgattc tgtggtgata aaaacaggag
      541 tgagagtaag caaggttgag aagatcgaca acatcttctc tgtaactatg ggagaggaga
      601 cagcaaaggc gactacagta ctcatcacct caggatcctc aaggaggaag ctcgacatcc
      661 ctggtgggaa ggagtttgag aacaagggta tcacctactg tgcatcttgc gacggtcccc
      721 tcttcgccga tatggataca gtagttatag gtggaggtaa tgctgggttc gagacagcag
      781 cacaactact ctcatacgcc aaatcagtga ccctcctaca cagagcagac aaatacagag
      841 ccgacgagat gactgtagag aaggtgctcg gtaatcctaa gatgaaaggc attcttaatg
      901 ctctaccaat cgaaatcaag ggtgacaaat tcgttaagtc tgttgtctat accgacaaaa
      961 tgactggaga gaatgtggag atccccgcat caggagtatt cgtcgagata ggtcttgtac
     1021 cgaacacctc ttttgtagaa ggagttgtgc ctatgaatga gttcaagcag atcattgctg
     1081 accctcgtac tggaaggact gaaacactag gtatctgggc cgcaggagat gtaacagaca
     1141 ccatgtacca ccagaacaac attgcatcag gcgatgctgt gagggcactc gagaatatat
     1201 acatgtatat aaggacaggt aggtagttaa tcaaaaaaat ataaaaagaa gagggcgggt
     1261 ccgtgaggac tcgcccttta ctaataccac cttcttctga tcagcgattg acgataaccg
     1321 tcgtactctg agtgttgtca ccaccacatt cgacgatgca ccacgccgtg agtacgactc
     1381 caaccgcgcc gtagatcctc ttgtgcttcc ccacgtgagt gaagaatcga tcgagtgtgg
     1441 acacccggtt gttaaccgag tagtcgatcc tgacgaccgt cgtgtcgcta tgtgtgatgt
     1501 gcgtcacgtg ttgcaccggc gcaccgctct ggcgaggcaa ctccgtctgg accggctggg
     1561 tctgagaagg agcaggctgc ggagcacgca ccacacgagg cggtgtacga ggcgcaccga
     1621 ccgactgctg ccgagcggtc gagcgaggct gtgaggccgt gctctgcgga caggtcgagt
     1681 tcgtcttcgg caacaggctc gagacaagag cggtgtcgcg acgggctgcc gtgtcgttcg
     1741 ggaccgtgta cacgaagttc ctgcagaact gcgtgctacc agtgtccttg ggtgcactcg
     1801 tagccgtctg agccgcgagg ccgatcggca tgaacgcgag gactgcgaga agaagattac
     1861 gagagaacat ccctacctcc gaaaagtggt ttgtgggttc tacgtatata ctataacaaa
     1921 atatctatct tgtaaaggcc taaagaaaaa ggcggaagag gagagatttg aactctcgag
     1981 acccttacga gcctgccggt tttcgaaacc ggtgccttca accactcagc cactcttcct
     2041 taggccaaat atagcatttt aggcataaaa tggtactata acgcatgttt atggccctat
     2101 cgtctatcgg ttaggacaca agcttttcaa gcttggaagc agggttcgac tccctgtagg
     2161 gtcacactgc gcggttagct cagttggtag agcatcccct tgacgtgggg agggtcagag
     2221 gttcgaatcc tctatcgcgc acagtgaaaa tacttgccat agagacttcg tgtgatgaga
     2281 ctgcaatatg ccttttagag tgttcgggtt cactcccttc acctaccttc aaagtcattg
     2341 cgaactcgct catatctcaa attgagatac acaaagagtt tggaggcgtc ttccccatgc
     2401 ttgccaagcg tgagcacacg aagaacttgc ccatcattct tgataaggtt ctgaaggacg
     2461 ctggagagga tccaaacaaa cccgacatag actacattgc tgtcacttcg ggccccggac
     2521 tcgagccagc tctttggacc ggcatagtat tcgctgaaga gctgagccaa aaatggggga
     2581 agaaagtaat acccatcaac cacatggagg gacacctctt ctcctcaata tttcagaata
     2641 cagacacact cgagctccct gctatcgcac tacttgtctc tggtggtcac accgagctcg
     2701 tattagtaga gagctttgat aaatttagaa tcattggtcg caccagggac gatgctgtgg
     2761 gagaagcctt cgacaaagtg gctcgcctcc tcgacctacc ctaccccgga ggacccaaga
     2821 tctcggagct tgctaacaga tcaagagcta ggagcaagga gggtactttc acctttcctc
     2881 gtccgatgat gaagacaagt gattacgact tctcctactc tggactcaag acctcggtac
     2941 tttatgctat acaaaaatta gattctataa ataatgagac caaagaagat attgctagag
     3001 gcttcgagga agcagctatt gaaccacttg tgtacaagac taagtcagct atagaagaat
     3061 ttggagtcgg aagtattatc ctcggtggtg gagtctcggc aaacacctat ctacaaagtg
     3121 agttaaagac attaagcaac gagtacggct tgccactaca tttacccaag tcagacctaa
     3181 gcactgacaa cgccataatg atcgctgttg ctgcttatat aaggattgta accgagagaa
     3241 tatcaatacc tgagaaaata gtcgcgaatg gtaatctcag cttagattcc aattagaagt
     3301 cgagcttgtc atcctctttg atctcaaatg caccagggag atcgattgta gtctcagtaa
     3361 catgagtctt cttcttaaag ctagagagtt tggttcttac agtgtcgtgc tttggataca
     3421 tagcaatcca gagcaggcta agtaccagcg ctatgattag gggtaggagt agacagaact
     3481 ccttgagcag aaaagctagg ataatagcga tagttaaacc gagtacaata gttgtccact
     3541 tgtaggcaaa cttcttaaag gacttttcta cacccttttt ataaaggaca tcggaaacta
     3601 tagacccaag gatataaagg acaaacagta ttagcgcaag ttcatataga cattgcattt
     3661 tgtcccttag agtttctggg aaggagaaga ctgctgcagc aaattggctg aattgtcctt
     3721 tatcctcctc tagctg
//
LOCUS       DAORIT010000027         3669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_5638_length_3669_cov_50.861372, whole genome shotgun sequence.
ACCESSION   DAORIT010000027 DAORIT010000000
VERSION     DAORIT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5638_length_3669_cov_50.861372"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..325)
                     /locus_tag="PLD99_02660"
     tRNA            complement(254..325)
                     /locus_tag="PLD99_02660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(290..292),aa:Arg,seq:gcg)
     gene            complement(348..611)
                     /gene="rpsT"
                     /locus_tag="PLD99_02665"
     CDS             complement(348..611)
                     /gene="rpsT"
                     /locus_tag="PLD99_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRH25817.1"
                     /translation="MPITRGAKKALRQSLKKRNFNRARKDGVSTAVKSLKKLIEDGKK
                     KDAQKALSLVQKALDKAVKGKTITKNTASRKKSRLSKMIKKIA"
     gene            635..1204
                     /gene="ruvA"
                     /locus_tag="PLD99_02670"
     CDS             635..1204
                     /gene="ruvA"
                     /locus_tag="PLD99_02670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRH25818.1"
                     /translation="MIGTISGTIRHKESNMLLVDVNGVGYKVFVTADTALELKMSDPC
                     FLWTHLAVRETALDLYGFLDKETLDLFELLIGISGIGPKTALGILTVASPAILRKAVA
                     NEDTSYLVKVSGIGKKNAEKIVLELRGKLLVGSDDKSSDMRSEGDVMEALISLGYNER
                     DIRDILKKMPKNIENTQDKVKYALKNLSN"
     gene            1214..2437
                     /locus_tag="PLD99_02675"
     CDS             1214..2437
                     /locus_tag="PLD99_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRH25819.1"
                     /translation="MLDKTLNFIKKFIPRSLFDALSPIYHYSLALVGAVLFGFPSRKL
                     TVIAVTGTKGKSSTTEIINSIFEAAGHKTALLNTIRFKIGDSSKPNKMKMTVPGRFFL
                     HMMLRKAQMAGCDTAVIELSSEAAKQYRHKFVYLDALVFTNLAPEHIESHGSFEKYRN
                     AKVSLVKNLKDKGVLVVNKDDENSIHFKQATDKKTFEWSTGDQFDYKTSLIGDFNKKN
                     ILASVALAKTFDISESDIKKGIENVSVIRGRAEFVKISDKQNFDVVVDYAHTSDSLKE
                     LYKAFEGKRKICVLGNTGGGRDKWKRPEMAKVADEYCDQVFLTNEDPYDEDPDQIIKD
                     MLPGFKKNNPKIIMDRRQAINTAIKNASAQSAVLITGKGTDPYIMLANNERLPWSDYE
                     IAREELKKILQNKTA"
     gene            complement(2470..2919)
                     /locus_tag="PLD99_02680"
     CDS             complement(2470..2919)
                     /locus_tag="PLD99_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25820.1"
                     /translation="MFVRVSVKFRNREISPLMFGPFNTKSDDVELLRLSVITERVQKL
                     VDRVNRTSGLITSKNLFDRFRLDVEPREDEDPLLPAISVRQIMVEQYLFQLEESLFAY
                     EPPLPQIDFKKKLTLWERVKKGFASFGRFLDDMGLEMAKNPNSHTWV"
     gene            complement(3352..>3669)
                     /locus_tag="PLD99_02685"
     CDS             complement(3352..>3669)
                     /locus_tag="PLD99_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25821.1"
                     /translation="IHLILVGRAHRPVVTHSNVHCEEKVATNNDKPNFQEQFHGAASS
                     YDPSTIGASDVPYTEAEAQKVKEDSLRAYKEEEARDQQRAEEQSDAWWANEMEDARRK
                     SEE"
BASE COUNT         1033 a          874 c          832 g          930 t
ORIGIN      
        1 aagaaatcag aaatatgatg gtaaaaaata tagaagaaga gtatcactca ctccccctcc
       61 tagagaaact cttggaatac ttaggattct agattgtgct cccagtagga cgctttgcta
      121 tctcactcct cggaaaccca tggttttccg aaaccttcct ccacgggaga ccagagtgtt
      181 ctcccgaccc ctctcctgtt cgattccaat aacactataa aaaactccgc gagaagcgga
      241 attttttata gtgtgctccc agtaggaatc gaacctacat tttaacttcc gcaaagtcat
      301 gtcctatcca ttgaacgatg ggagcgtcaa accattctaa cccaaaccta tgctatcttc
      361 ttgatcatct tcgaaagacg agacttcttt ctagatgcag tgttcttggt gatggtctta
      421 cccttcaccg ccttgtctag cgccttctgc acgagactga gtgctttttg agcatccttc
      481 ttcttgccat cttcgatgag cttctttaga gacttaacgg cggttgagac accgtctttc
      541 ctagcacgat taaaattgcg cttcttgagc gattgtcgga gagccttctt ggcaccgcgt
      601 gttattggca tgagggagat gctatcatac caagatgatt ggcacaatat ctggcaccat
      661 aaggcacaaa gagtcaaata tgctccttgt cgatgtaaac ggtgtgggtt ataaggtttt
      721 tgtcacagcg gatacggctc tagagctcaa aatgagtgat ccatgcttcc tctggaccca
      781 tcttgcggta agggaaaccg ctctcgactt gtacggtttc cttgataaag agacactaga
      841 cctcttcgaa ctcctcattg gtatatctgg tattggaccc aagactgctc ttggcattct
      901 cacagtcgcc tcccctgcca tacttaggaa ggctgtagca aacgaagaca ccagctacct
      961 tgtaaaagtc tcgggcatcg gcaaaaagaa tgccgagaag attgtgttag aactccgtgg
     1021 caaactactc gttggatcag atgacaagag cagtgacatg cgctctgagg gtgatgtaat
     1081 ggaagcactc atatctcttg ggtacaacga gcgagacata cgagacatac tgaagaaaat
     1141 gcccaagaat attgaaaata cccaagacaa agtaaagtac gctctaaaga acctatctaa
     1201 ttaacaaatt cgaatgctag ataagactct aaactttata aagaagttca taccacgaag
     1261 tctctttgac gcactttctc caatctacca ctactcgctc gccctggtgg gtgcagtact
     1321 cttcggcttt ccttctagga aattaactgt gattgccgtt acaggcacta aaggaaagtc
     1381 atctaccacc gagattatta attcgatttt cgaagcagcg ggacataaga ctgctcttct
     1441 aaatactatt cggttcaaaa ttggcgactc atcaaaacca aacaaaatga agatgacagt
     1501 ccctggacgt ttcttcctcc acatgatgct ccgcaaagcc caaatggccg gatgcgatac
     1561 tgcggttata gagctctcaa gtgaggcggc taagcagtat agacacaagt ttgtatacct
     1621 cgatgctttg gtcttcacca acctcgcacc cgagcacata gagagtcatg gctcgtttga
     1681 gaagtatcga aatgcgaaag taagtctggt aaagaatttg aaagacaagg gagtgctcgt
     1741 ggtaaacaaa gatgatgaga actcgatcca tttcaaacaa gctacggata aaaagacttt
     1801 tgaatggagt acgggcgacc agttcgacta caaaactagt ttgattggag attttaataa
     1861 gaaaaacatt ttggcctctg tagcgctggc taagacattt gatataagtg agagtgatat
     1921 aaaaaaaggg attgagaatg tttctgtcat caggggtcgc gcagaatttg taaagataag
     1981 tgacaaacaa aattttgatg tggtcgttga ttacgcacac acatctgact ctttgaaaga
     2041 gttgtacaaa gcctttgaag gcaagagaaa gatctgtgta cttggtaaca ccggaggcgg
     2101 gagagataaa tggaagagac ccgagatggc caaagttgcc gatgagtact gtgatcaagt
     2161 gtttctaacc aatgaagacc cgtacgacga agatccagac cagatcatta aagatatgtt
     2221 gccaggattc aagaaaaaca atcctaaaat tattatggat aggcgtcagg ccataaacac
     2281 agcaatcaaa aacgcaagtg ctcaaagtgc agtgcttatt accggtaagg gtactgaccc
     2341 atacataatg ctagcgaaca acgagagact accttggagc gattatgaaa ttgcgcgcga
     2401 agaactaaag aaaattttgc aaaacaaaac cgcgtaagta tctacgcggt tggcacagct
     2461 agtccggtgt tagacccatg tgtgcgagtt cgggttcttt gccatctcga gacccatgtc
     2521 atcgagaaag cgtccgaagg acgcgaagcc cttcttcacc ctctcccaga gtgtgagttt
     2581 cttcttgaag tcgatctgag gaagcggtgg ctcgtaggcg aacagactct cttccaactg
     2641 gaagaggtac tgctcgacca tgatctgacg aacgctgatc gcaggaagaa gagggtcctc
     2701 atcctctctc ggctcgacat caagcctgaa ccgatcgaag aggttcttgc tggtgatgag
     2761 gcccgaggtc ctgttgaccc tgtcgaccag tttttgcact cgctcagtga tgacactgag
     2821 tcgtagcagc tccacgtcat cactcttcgt gttgaacggt ccgaacatga gaggactgat
     2881 ctctctgttt cgaaacttga ccgaaaccct gacgaacata acgcctcctt gaatgtgttg
     2941 gtgcttatat ctacatataa tagtaccaca ctaaacaccc aaaagtcaag tttagattag
     3001 cgttgacgtg tagccatgat tttggcaagg taaatacaga ctaccaccgt gcattcagtt
     3061 gcacacaaag gaggtgagaa agatggagca gaagccaatc gagatcgccc cgatcgtgca
     3121 cacgacccct tccggagaag ccgtcttcaa gggggctggc aacagccact tcagccatgt
     3181 gcccggccag gggttccacg tcacgaccga tgtccccggg ttccctcgcg gatcctcgga
     3241 ccacaccgag gtcaagacga agtagccaga cacggccatc ccacacaaca cattgtgtgg
     3301 gattttcttt ataaaaaacc cgcacaatat acatgcgggg gtcactttca actactcttc
     3361 acttttgcgg cgagcatctt ccatctcatt tgcccaccac gcatcactct gttcttcagc
     3421 cctctgctga tctcgtgctt cttcctcctt gtatgcacgg agactgtcct cctttacttt
     3481 ctgtgcttct gcttcagtgt atggaacatc actagcccct atggtactag ggtcataact
     3541 ggaagcggca ccatgaaatt gttcctgaaa attgggttta tcattatttg tggccacttt
     3601 ctcctcacag tgtacgtttg aatgggtcac caccgggcgg tgggccctgc ctacgaggat
     3661 caggtggat
//
LOCUS       DAORIT010000028         3610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_5818_length_3610_cov_54.212940, whole genome shotgun sequence.
ACCESSION   DAORIT010000028 DAORIT010000000
VERSION     DAORIT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5818_length_3610_cov_54.212940"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1082
                     /gene="mutM"
                     /locus_tag="PLD99_02690"
     CDS             222..1082
                     /gene="mutM"
                     /locus_tag="PLD99_02690"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HRH25822.1"
                     /translation="MPELPEVETTVRGLNRTVRGRKIVSVSTVYKSAYHKDKDNIKDP
                     KFFKLFKSKVIGQKILRAERRAKNILMHLDNGQTILTHMKMTGFYFYNPPSDAKFIRL
                     NFELDNGKHLAFSDMRKFAKVTLIETSKLTHSPHLSHLGPEPLDKKFQLVKFVEQLNK
                     RADTPIKTVLMDQTLIAGIGNIYSDEILWRAGIHPSSKPKAIPKKNLILAFKAMKEVL
                     KKGIDFGGDSMSDYKNIDGKSGKFQEHHQAYRRTGEKCFKRGCIGIMQRLVVGTRSAH
                     FCPVHQTQYE"
     gene            1111..1392
                     /locus_tag="PLD99_02695"
     CDS             1111..1392
                     /locus_tag="PLD99_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25823.1"
                     /translation="MKTLIIAILFLVPVASFAGNTTVTYPVKDKTTTTATYDKTKTTT
                     TYDKTTKYTGTVMSEAERQQEMIRLQNLIAQLLQQLIALLQAQQKSLGQ"
     gene            complement(1381..1851)
                     /locus_tag="PLD99_02700"
     CDS             complement(1381..1851)
                     /locus_tag="PLD99_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRH25824.1"
                     /translation="MNKTCVLVLDNIRSVENVGSIFRTAEGLGASKLYLIGTTPTPLD
                     RFGRKRSDMAKVALGAEETLEWEYRENLVEVLDELKSQNFQIISLEQSERSSDLKSFK
                     PQDVFALIVGNEVDGVSKEVLEMSDDIVEIEMRGEKESLNVSVSTGIALYQLLS"
     gene            1869..2222
                     /locus_tag="PLD99_02705"
     CDS             1869..2222
                     /locus_tag="PLD99_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25825.1"
                     /translation="MLNPFPDLLIYSMLAPFILRIVLGLVYLDLGTLNWKKSGNMKIL
                     GLVEIIGAAMLFFGIYTQYAAMLFILIGGISFYIEYKDASVLKRDIVFYLLVLAISIS
                     LLLTGAGAYAKDLPL"
     gene            2267..2337
                     /locus_tag="PLD99_02710"
     tRNA            2267..2337
                     /locus_tag="PLD99_02710"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2299..2301,aa:Lys,seq:ttt)
     gene            complement(2445..>3610)
                     /locus_tag="PLD99_02715"
     CDS             complement(2445..>3610)
                     /locus_tag="PLD99_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRH25826.1"
                     /translation="PRRPSWFGRNRNFARKNFAHTLMKRILFATILLVIGFNHVNAQE
                     VFSEYQGTWRAEVLDILKIESRIIPGTENSENIQTLSARIIDGPKKGEVVTIKNDFLD
                     LKKGSKFYFSYNIDVGGVRSYNALSVDRRGSLIFLIAIFSLAVVVFGGFQGLRSLLAL
                     AGSFLAVFYILIPGLLDGWHPLIASFSVAAGILFAAIFFTHGLNKESLVAYTGTMIAV
                     ILTSVFALIAVKMTSLTGFATDEAVYLDFDTKGALDFTSILLGAIIIGVLGVLDDIAI
                     TQAAVVTELYGSNPSLSKFEVYRRAIRIGREHVGALVNTLVLAYTGASLPLLLYFSLS
                     TSTLGSIMNTEIIATEIVRAVVGSLGLILTVPIVTLLAVFYLKDYKGSHSHHH"
BASE COUNT         1106 a          858 c          711 g          935 t
ORIGIN      
        1 cgtttgaatg ggtcaccacc gggcggtggg ccctgcctac gaggatcagg tggatcaggg
       61 aacttcgtgg gacgaggtgg tgtgggcgga gggggcaact tgcctccatc cgtcgtcttg
      121 ggctcgcttc ctgacacaaa actctccttg aaagggcgtg ggtaggtcta taaacaaact
      181 acctatctat taaatttttg caaataatat aagctaacct catgccagaa ctaccagagg
      241 ttgagacaac agtaaggggg ttaaatagga cggtccgagg caggaagatt gtcagtgtga
      301 gcactgtata caagagtgcc taccacaagg ataaagacaa catcaaagac cctaaattct
      361 tcaagctctt taaaagtaag gtcattggac aaaagatttt aagggcagag agaagagcca
      421 aaaatatttt gatgcatttg gataatggtc agacaatcct cacccacatg aagatgactg
      481 gcttctactt ctacaatcca ccttcggatg ctaaattcat ccgacttaac tttgagttag
      541 ataacggcaa acatctcgct ttttccgata tgagaaagtt tgccaaggtg accttgattg
      601 aaacttccaa actcactcac tctcctcacc tctcgcattt gggccccgag ccgttagaca
      661 aaaaatttca actagtaaaa ttcgtcgagc aattaaacaa gagagcagac acgccgatca
      721 aaacagttct gatggaccag actctcattg ctgggattgg caatatttat tctgacgaga
      781 tcctctggcg cgccggcata caccctagct ctaaacctaa agcgataccg aagaagaacc
      841 tgattctcgc cttcaaggca atgaaagagg tcttgaagaa aggaatcgac ttcggcggag
      901 actcgatgag cgactataag aacatcgatg gcaagagtgg taaattccaa gagcatcatc
      961 aagcatatag acgtactgga gagaaatgtt ttaagcgcgg gtgcattggt ataatgcaaa
     1021 gactagtagt tggaacccgt agcgcccact tctgccctgt gcatcaaact caatacgaat
     1081 aagttataat tgagagacaa caacaaaaat atgaaaacat taattatcgc aatattattc
     1141 ctagtcccag tagcaagctt tgctggtaac accacagtaa cttatccggt gaaggacaag
     1201 accaccacaa ccgctacata cgataaaacc aagaccacca ctacttacga caaaacaact
     1261 aaatacacag gcactgttat gagcgaggcg gaaagacaac aagagatgat aagactccag
     1321 aatcttattg ctcaacttct ccaacaattg atcgcacttc tacaggctca acaaaagtcc
     1381 ttaggacagt aattgataaa gcgcgatacc agtagagact gagacattca gagactcttt
     1441 ctcccctctc atctcgatct cgacaatatc atcggacatc tctaacactt ccttcgatac
     1501 tccgtctact tcgttaccta caattaatgc aaacacatct tgtggcttaa aagatttcag
     1561 gtcactagac ctctcacttt gttcgagaga aataatttga aagttttgag acttaagctc
     1621 atccaagacc tcaacgagat tttctctata ttcccactca agtgtctcct ctgctcctag
     1681 agcaaccttg gccatatcag agcgcttacg accaaagcgg tctagaggtg taggtgtagt
     1741 gccaataaga tatagcttgg atgccccaag accttccgcg gtgcggaaga tcgagcctac
     1801 gttctctacg cttcttatgt tatctaagac tagtacacag gttttgttca tggtgctaag
     1861 attacactat gttaaatcca tttccagacc tgctcattta ttcgatgctt gcgccattta
     1921 tccttaggat tgtcctcggc ttggtctacc tagaccttgg aacccttaat tggaagaagt
     1981 caggcaacat gaagatcctc ggcctagtag agataatagg cgcggcaatg ctcttttttg
     2041 gcatctacac acaatacgcg gcaatgctct ttatccttat tggtggcatt agcttctata
     2101 tagagtacaa agatgcttca gtcctcaagc gtgatatcgt cttctacctt ctcgtcctcg
     2161 ctatctccat ctctctcctc ctcaccggtg caggcgctta cgccaaagac ctgcctctct
     2221 aaaatcctag ctttagagcg agaattattg tattttaaag acctaagctc ccatagctca
     2281 gtggtagagc agttgccttt taagcaattg atcatggttc gattccatgt gggagcacac
     2341 aataaagtaa aaaattcttc ttggtcagca cctcaatctc agccagctcg cggtgagagt
     2401 cgtgacaact ggtcactaag accatttcga tcttaataat gagctcagtg gtggtgacta
     2461 tgactcccct tgtaatcctt aagataaaag accgctagaa gtgtcactat cggtacggtt
     2521 aatataagtc caagtgaccc aaccacagcc cttactatct cagtggcaat tatctcagtg
     2581 ttcataattg atccaagtgt tgatgtagaa agactgaagt aaagcaaaag aggtaatgag
     2641 gctcctgtat atgccaaaac aagtgtgttt accaaggcac caacatgttc acgaccaatc
     2701 ctgattgctc gcctataaac ctcaaactta ctaaggcttg gattactccc atacaactca
     2761 gtcacaacag cagcttgagt aatagcaata tcatcaagca ctccgagcac tccgataata
     2821 attgctccga gaagtatgga tgtaaagtct agggccccct tagtatcaaa atcaagataa
     2881 acagcctcat cagttgcaaa gccagtcaga ctagtcatct tcacagcaat gagagcaaaa
     2941 acgctagtga gaataaccgc gatcattgtg cccgtatatg caacgagtga ttccttattc
     3001 agcccatgag tgaagaatat cgctgcaaac aaaatacccg ctgccacaga gaagctcgct
     3061 ataagtggat gccaaccatc aagaagtcca ggtatgagga tatagaatac tgccagaaaa
     3121 ctccctgcca gagccaaaag agatctcagt ccctgaaaac cgccaaacac taccaccgcc
     3181 aaactaaaaa ttgctattag aaagatgaga gaccctctcc ggtctacact cagggcgtta
     3241 tagctgcgaa ccccaccaac atcaatgttg taactaaaat agaacttgct accctttttg
     3301 agatccaaga aatcattttt tatcgtaaca acttccccct tctttggacc atctataatt
     3361 cttgcgctta atgtttgtat attctcgctg ttttcagtac caggaattat tcttgattct
     3421 atcttcaaaa tatcaaggac ttctgctctc catgtaccct gatactcact aaagacctct
     3481 tgtgcattaa catgattaaa accaattacg agaagtattg tggcaaataa gattcttttc
     3541 atcagagtgt gagcaaaatt ttttcgagca aagtttctat ttctaccaaa ccacgacggg
     3601 cgtcgtggta
//
LOCUS       DAORIT010000029         3320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_6811_length_3320_cov_54.826953, whole genome shotgun sequence.
ACCESSION   DAORIT010000029 DAORIT010000000
VERSION     DAORIT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6811_length_3320_cov_54.826953"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..858)
                     /locus_tag="PLD99_02720"
     CDS             complement(1..858)
                     /locus_tag="PLD99_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRH25827.1"
                     /translation="MSKDYYKILGVERGASEDEIKKAFRKLAHQYHPDKKSGDEAKFK
                     ELNEAYTVLSDKQKRAQYDNFGSTGGMGGGGQGFNPNDFGFDFSNFSQGGFEGGDLGD
                     ILSSIFGGRRVRRGRDIAVDIELSFHESVFGADRKVVINSKLVKQKEVAISVPPGIDD
                     GQMIRMSAMGETVEGGVPGDLYVKVHVRRHPTLKKEGYNLLMDLQIKLSEALLGAEHR
                     IATLDGDIMLKVPQGTNTGTILRVKGKGVPMSKDKRGDLYVRVKVTIPEKLSKDAKKL
                     IEELKKEGI"
     gene            complement(858..2744)
                     /gene="dnaK"
                     /locus_tag="PLD99_02725"
     CDS             complement(858..2744)
                     /gene="dnaK"
                     /locus_tag="PLD99_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRH25828.1"
                     /translation="MAKIIGIDLGTTFSAVAVMEAGSPKIMENSEGARTTPSIVAVSK
                     TGERLVGLLAKRQAVTNPKNTVFGIKRFIGHTFAEPSTQKDKAVVPYEVKEGPQGGVL
                     VKMNDKDYRPEEISAMILQKIKTDVEARLGEKVTEAVITVPAYFNDAQRQATKDAGQI
                     AGLDVKRIINEPTAAALAYGLNKKKNEKIAVFDFGGGTFDISILEVGDDVVEVKSTDG
                     DSHLGGKDIDQKILNWIADEYKKESGIDVRGDALALQRLDEAAEKAKIELSSAVETEI
                     NIPFITSDASGPKHLLLKMNRAKLEELAQEFVDRAMTITKRAVESSPFKVGEINEVLL
                     VGGQTRMPKIAESVKNFFGKEPNKSINPDEVVALGAAVQGGIIAGDVKDILLLDVIPL
                     SFGIETMGGVATKLIEKNTTIPAAKSQIFSTAADNQPSVEIHIVQGERAMASDNKSIG
                     RFVLDGIPPSPRGLPQIEVTFDIDANGILQVKAKDKSTGKEQSIRIEASSGLSKEEIE
                     KMQKDAEMNAAEDEKKKSLVDAKNLSEQLIYTAEKGLKDAPTLPEDIKTGVNGKIEDL
                     KKVKDGTDLEAIKSASEALSTEMQKIGEYLNKNQSAPNSNEPKTAENTENPDIKDADT
                     SEAK"
     gene            complement(2755..>3320)
                     /locus_tag="PLD99_02730"
     CDS             complement(2755..>3320)
                     /locus_tag="PLD99_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRH25829.1"
                     /translation="KPVIMRAIMQENDDIQIDSESDLDDSVLAEEHQGDTIKKLREKL
                     KLAEEKAKEYLNELQRAKADFVNLRKRDEDDRVEFIKFAKSDVVSELIPVLDSFTLAE
                     AHGDEGSKPIHKQLLKILKTHGLEEVNPLHEVFDPKHHEAIGVVETENPEEDNQILEV
                     VQKGYILSGKTLRPAIVKTGEYKINRY"
BASE COUNT          668 a          862 c          746 g         1044 t
ORIGIN      
        1 ttatatccct tcttttttga gttcttcgat gagtttcttg gcgtccttag agagcttctc
       61 ggggatggtt actttgacgc gtacatagag gtcgccacgc ttatccttgc tcataggtac
      121 tcctttaccc ttaaccctaa ggattgtgcc agtattcgtg ccttgaggca cctttagcat
      181 gatgtcgcca tctagggtgg ctatgcggtg ctcggcgcct agtagggcct ctgagagctt
      241 gatttggagg tccataagga ggttgtagcc ctctttcttt agggttgggt ggcgacgcac
      301 atgcaccttc acatacaaat cgccagggac tccaccttct acagtctctc ccatagcaga
      361 catgcggatc atctgaccat cgtctatacc tggaggcacg cttatagcca cttccttctg
      421 ctttacaagc ttggagttga tcactacctt tctgtccgca ccaaagactg attcgtggaa
      481 tgagagttct atgtccactg caatgtctct gcctctcctt actcgtctac cgccgaaaat
      541 tgatgagagt atgtcaccca agtccccacc ctcgaatcca ccttgcgaga agttggagaa
      601 gtcgaaccca aagtcgtttg gattgaatcc ttgtcctccc ccgcccatgc caccagtact
      661 accgaagttg tcgtactgag cacgcttctg tttgtcagag agtactgtgt aggcctcgtt
      721 caattccttg aacttagctt cgtcgccaga cttcttatct ggatgatatt gatgagcgag
      781 tttgcgaaag gctttcttga tctcgtcttc tgaagcacct ctctcaactc ctagaatctt
      841 atagtagtct ttagacatta tttagcttct gatgtatcag catccttaat gtctgggttt
      901 tcagtatttt ctgctgtctt tggttcgttt gaatttggtg ctgattgatt tttgttcagg
      961 tactcaccaa tcttttgcat ctcagttgaa agagcctcgc tagcagattt gatagcctct
     1021 aggtcagtac catccttaac tttctttaag tcctcgatct tcccattaac acccgtcttg
     1081 atatcttcgg gtagagttgg tgcgtcctta agacccttct cagctgtata gatgagttgc
     1141 tctgagaggt tcttggcatc caccaaactc ttcttcttct catcttccgc agcattcatc
     1201 tctgcgtcct tctgcatctt ctcgatctcc tccttagaga gtccacttga tgcttcaata
     1261 cggattgatt gctccttgcc agtagattta tccttcgcct ttacttgtag gataccgttt
     1321 gcatctatgt cgaaggtaac ctcgatctgt gggagaccgc gagggcttgg tggtatgccg
     1381 tcgaggacga agcgaccaat agacttgttg tcagaggcca ttgctctctc gccttggacg
     1441 atgtggatct cgactgatgg ttggttgtcg gctgctgtgg agaaaatctg tgacttagca
     1501 gctgggatgg tggtgttttt ctcgatcaac ttggtagcga ctccacccat agtctcgata
     1561 ccaaatgaga gtggtataac atcgagtagt aggatgtcct tcacatctcc tgcaatgata
     1621 cctccttgca ctgcagcacc tagagccaca acctcgtctg ggttgattga tttgttaggc
     1681 tctttaccga agaagttctt cacgctctcg gcgatcttag gcatcctggt ctgacctccc
     1741 acgagtaaca cttcgttgat ctctccaact ttgaatggag acgactcgac cgcgcgctta
     1801 gtgatggtca tagcacggtc gacgaactcc tgtgcaagct cctccaactt agctctgttc
     1861 atcttgagga gtaggtgctt tggacctgat gcatctgagg taatgaatgg gatgttgatc
     1921 tcggtctcta cagcacttga gagctcgatc ttcgctttct ctgcggcttc gtctaggcgc
     1981 tggagtgcta gtgcgtctcc acggacatct atgccagact ccttcttgta ctcgtctgcg
     2041 atccagttaa ggatcttctg gtcgatgtcc ttacctccaa ggtggctgtc accgtctgta
     2101 gacttaacct caaccacatc gtcaccgact tctagtatag aaatatcgaa tgtgccccct
     2161 ccgaagtcga agactgctat cttctcgttc ttcttcttgt ttagaccata tgcaagagca
     2221 gctgcagtag gctcgttgat gatgcgcttc acgtccaagc ctgcaatctg accagcgtcc
     2281 ttagtagctt gtctctgtgc atcgttgaag tatgctggca cagtgatcac tgcttccgtc
     2341 accttctctc ctagtcttgc ctctacgtcg gtcttaatct tctggagtat catggccgag
     2401 atctcctctg ggcggtagtc cttgtcattc atcttaacga gcactcctcc ttgaggaccc
     2461 tccttaacct cgtaaggcac aacagcttta tccttctgag tagaaggttc tgcgaaggtg
     2521 tgaccgatga agcgcttgat accaaagact gtgtttttag gattggttac tgcctgcctc
     2581 ttcgcgagta gaccgacgag tctctctccg gtcttagaca cagcaacaat agagggtgta
     2641 gtacgagcac cctcgctgtt ctccattatt tttggactcc cagcttccat aactgcgact
     2701 gctgagaagg ttgtacctaa gtcgatgccg attattttag ccatggttgt gttgttagta
     2761 tctattaatc ttgtactctc cagtttttac aatagcggga cggagagttt tgcctgatag
     2821 tatataacct ttctgcacta cttcaagaat ctgattatct tcctcgggat tctctgtctc
     2881 taccacccct atcgcttcat gatgcttcgg gtcgaagacc tcgtggagcg gattcacctc
     2941 ctctaggccg tgggtcttga ggatctttag aagttgcttg tgtattggtt tggatccttc
     3001 gtctccatga gcctcagcga gggtgaagct atcgagcact gggatgagct cgctcactac
     3061 gtcacttttc gcaaacttga tgaactcgac cctatcgtcc tcgtctctct tacgcaggtt
     3121 gacgaagtcg gccttggcac gctgcagttc atttaagtat tctttggcct tctcctcggc
     3181 tagcttgagc ttctccctca gtttcttaat agtgtcacct tgatgctctt cggctagcac
     3241 ggagtcgtcc aaatccgact cagaatctat ctgaatatca tcgttttcct gcataattgc
     3301 ccgcattata accggttttt
//
LOCUS       DAORIT010000030         3272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_6992_length_3272_cov_53.930370, whole genome shotgun sequence.
ACCESSION   DAORIT010000030 DAORIT010000000
VERSION     DAORIT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6992_length_3272_cov_53.930370"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..74)
                     /locus_tag="PLD99_02735"
     tRNA            complement(3..74)
                     /locus_tag="PLD99_02735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Arg,seq:cct)
     gene            complement(148..220)
                     /locus_tag="PLD99_02740"
     tRNA            complement(148..220)
                     /locus_tag="PLD99_02740"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Phe,seq:gaa)
     gene            complement(222..452)
                     /locus_tag="PLD99_02745"
     CDS             complement(222..452)
                     /locus_tag="PLD99_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25830.1"
                     /translation="MNPEEKHQFERLLKLTEENNEMLRKIRRNTKWTFVWGIVKIAVF
                     VVPFFIAYFYLEPYLGTFGDTLKQAQDLIKGV"
     gene            complement(491..943)
                     /locus_tag="PLD99_02750"
     CDS             complement(491..943)
                     /locus_tag="PLD99_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRH25831.1"
                     /translation="MTKRSFFERLTGAVKLDEETYESELPATQTQKQNIFEDEEEEGQ
                     LTVDVYETPTEIVLKTMVAGVKPEDLDVAISREAVTIRGKREEDRTVAKEDYFHRELY
                     WGTFSRTVVLPAEIDVDEADAIEKYGLLILRLPKLDKNRQTKLKVKGN"
     gene            complement(968..1684)
                     /locus_tag="PLD99_02755"
     CDS             complement(968..1684)
                     /locus_tag="PLD99_02755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRH25832.1"
                     /translation="MTNSIVTFESVMYAFLGGVLPAILWLLFWLREDAKRPEPKGRII
                     ETFIAGMLSVLVVLYLQREIFYFSYYGVFPFLVWGTLEELVKLGSAYSTALRSKDDNE
                     PIDPLIYMITAALGFVALENTLFILNPLINEGLILAEQSVPIAVNTGATRFLGASLLH
                     ILASGAIGVALSLAFYKSRSKKILYGFIGLLVAMAIHTTFNAFVYKYFDTHSLVIFGS
                     VWFGVAVLLLIFEKIKSIAP"
     gene            complement(1674..>3272)
                     /locus_tag="PLD99_02760"
     CDS             complement(1674..>3272)
                     /locus_tag="PLD99_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRH25833.1"
                     /translation="MYDDGLIYRGSRIVNWDPKGQTVISDDEIVYEEEKSTLYYLKYG
                     PFTIATARPETKFGDKYVVMHPEDARYKEYEHGQKIELEWINGPITATVIKDESIDMD
                     FGTGVMTITPWHDVTDFEIAERHSLDKEQIIDKFGKLLPVAGDFAGLKINDARERIVE
                     RLREKGLLIKDEPYTHNIATAERTGGRIEPQIMKQWFINVNKPIEARGGKSLKELMRE
                     PVENDDIKILPEYFDKTYFHWIDNLRDWCISRQIWYGHRIPVWYKGEEIYSGIEAPEG
                     DGWMQDEDTLDTWFSSGLWTFSTLGWPEKTKDLETFHPTDVLETGHEILFFWVARMIL
                     MTKYVIGENPFKVVYLHGTVRDGQGKKMSKSLGNGIDPLDVAKEFGADAGRMALVVGN
                     TPGTDTRISNDKIKAYKNFANKIWNITRFVLTQENKGELKTDLINEFNALVKETTLDM
                     DNYRFYLAAEKIYAYVWHRFADEIIEESKGKEDYSATLYYILNNSLKLLHPFMPFVTE
                     EIWQTLYNGERILMVEPWPTTNDK"
BASE COUNT          835 a          768 c          701 g          968 t
ORIGIN      
        1 ggtgctctca gtaggaatcg aacctacatc tcatccttag gacggatttg ctctatccat
       61 tgagctatga gagcttgagg acaatatagc acactgactt tctatccaca gcattccaca
      121 cgttttccac aggtttatcc acaaatgtgc ttgggaccag gttcgaactg gtgacccttc
      181 cctcttcagg ggaatgctct accaactgag ctacccaagc attaaactcc cttaatcaag
      241 tcttgagcct gctttagcgt gtcaccaaat gtgccaagat aaggctcaag atagaagtag
      301 gcaataaaga acggaactac gaagactgca atctttacga ttccccagac gaaggtccat
      361 ttggtatttc gacggatctt acggagcatc tcgttattct cctctgttag cttaaggagt
      421 ctttcgaact gatgcttttc ctcgggattc atggggagat tctaccagaa agtggctaaa
      481 actcaataaa ttagttgccc ttaaccttta gcttggtttg tcggttcttg tcgagcttgg
      541 ggaggcgaag tatgagtaga ccatactttt ctatagcatc tgcttcgtcc acatcgatct
      601 ctgcagggag cacaacagtg cgagagaagg tgccccaata tagctctcgg tggaagtagt
      661 cctcctttgc cacagttctg tcttcttcgc gtttacctcg gatggttact gcctcacggc
      721 tgatagccac atctaagtct tcaggcttaa caccggctac catggtttta aggacgatct
      781 cagtaggtgt ctcgtacaca tcgacagtta gctgaccctc ttcttcctca tcttcgaaaa
      841 tattttgctt ctgtgtttgg gtggcaggaa gttcgctctc gtatgtttcc tcatcgagct
      901 taaccgcacc agtcaatcgt tcaaaaaaag atctttttgt cataggttta attatatctc
      961 tatattgcta tggtgcaatt gatttaatct tttcgaaaat tagtaggagc accgctacac
     1021 cgaaccaaac gctgccaaag ataactaagc tgtgagtatc gaaatatttg tagacgaagg
     1081 cgttgaatgt tgtgtgtata gccatggcta ccagtagtcc aataaatccg tatagtattt
     1141 tcttgctgcg actcttatag aaggcaagtg aaagggcaac accgatggcg ccagaggcca
     1201 agatgtggag gagactcgca ccgaggaatc tggttgcacc agtgttcacc gctataggta
     1261 cactctgctc agcgagaata agaccttcgt ttatgagtgg gttcaatata aagagagtgt
     1321 tttcaagtgc gacgaaacca agagcggctg taatcatgta gattagtgga tctataggtt
     1381 cgttatcatc tttacttctt agagctgtgg agtaagcact tccaagtttg accaactctt
     1441 cgagggtccc ccaaacgagg aagggaaaca ctccatagta tgagaaatag aaaatctctc
     1501 tttgtaagta aagaacgacc aagactgaaa gcatgccagc aatgaaggtc tctattatcc
     1561 taccctttgg ttctggtctt ttcgcgtctt ctcttagcca gaagagtagc cagagtatgg
     1621 cggggagcac acctcctaag aaggcgtaca taacagattc gaaggtgacg atgctatttg
     1681 tcatttgttg ttggccaagg ttccaccatt aggattctct ccccattata aagtgtttgc
     1741 cagatctctt cggtaacgaa tggcataaat gggtgcaaga gcttaagaga gttgtttagt
     1801 atgtaataaa gagtagcact atagtcttcc ttacccttac tctcctctat gatctcatct
     1861 gcgaagcgat gccatacata cgcatagatc ttttctgctg cgaggtagaa gcggtagttg
     1921 tccatatcga gtgttgtctc ctttaccaaa gcattgaact cattaatcaa gtcagtctta
     1981 agttcgccct tgttttcctg cgtcagaaca aatcgagtaa tgttccaaat cttattagcg
     2041 aagttcttgt atgctttgat cttgtcatta gaaatacggg tgtcggtccc gggagtattg
     2101 cctacaacta gagccattct gccagcatct gcaccaaatt ccttagccac atcaagaggg
     2161 tctatgccgt ttcccaagga tttgctcatc ttcttgccct gtccatcacg tactgtgccg
     2221 tgcaggtaca ctaccttaaa tgggttctca ccaatgacat acttggtcat aaggatcatt
     2281 cttgctaccc agaagaagag gatttcgtga ccagtctcga gtacatctgt agggtggaaa
     2341 gtctccaagt ctttagtttt ctcaggccag cccaaggtag agaaagtcca aagccctgaa
     2401 gagaaccatg tgtccaaggt gtcttcgtcc tgcatccaac catcaccttc aggtgcctca
     2461 atgccagaat atatttcctc tcccttatac caaacaggaa ttctgtggcc gtaccagatt
     2521 tgtcttgaga tgcaccaatc acgaaggtta tctatccagt ggaagtaagt tttgtcgaag
     2581 tattctggaa gtatcttaat gtcatcattt tccacgggtt ctctcataag ttccttcaaa
     2641 ctcttgcctc cgcgggcttc tattggcttg ttcacattga tgaaccactg cttcattatc
     2701 tgtggttcga tcctgccacc ggtgcgttct gctgtggcta tattgtgagt ataaggctca
     2761 tccttgatga ggagaccctt ctctctcaat ctctcaacaa ttctctctct tgcgtcatta
     2821 atctttaggc cggcgaagtc accggcaaca ggcagtaact tgccgaactt atctataatc
     2881 tgttctttat ccaaagagtg tctctcggcg atctcgaagt cagtaacatc gtgccagggg
     2941 gtaatagtca tcactccagt accgaaatcc atatcgatag attcatcctt aatgacggta
     3001 gctgttatag gtccatttat ccactcaagt tcgatcttct gaccatgctc atactctttg
     3061 tagcgggcgt cttctggatg cataacaaca tatttgtcac caaattttgt ttccggtctg
     3121 gctgtggcaa tagtgaaggg cccgtacttc aagtaataaa gcgtagactt ctcttcctcg
     3181 tatacaattt cgtcgtccga gatcactgtt tgacccttgg ggtcccagtt aactattcga
     3241 ctaccacgat aaatcagacc gtcgtcgtac at
//
LOCUS       DAORIT010000031         3022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_8153_length_3022_cov_53.298281, whole genome shotgun sequence.
ACCESSION   DAORIT010000031 DAORIT010000000
VERSION     DAORIT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8153_length_3022_cov_53.298281"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..260
                     /locus_tag="PLD99_02765"
     tRNA            188..260
                     /locus_tag="PLD99_02765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:221..223,aa:Ala,seq:tgc)
     gene            319..564
                     /locus_tag="PLD99_02770"
     CDS             319..564
                     /locus_tag="PLD99_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25834.1"
                     /translation="MYRITSWRFDVRSTTHSTPEFEGETVEDCDRQALAHFDEVSAKP
                     DNAWECMDIARIDVPAVAEKVTRLAKNENAKQDELRD"
     gene            complement(620..796)
                     /locus_tag="PLD99_02775"
     CDS             complement(620..796)
                     /locus_tag="PLD99_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25835.1"
                     /translation="MTRPIQPTPTVRGKDAIEILREIRAGTPLTPERAELFRRADEIF
                     RNSGHAFRINREPK"
     gene            891..1052
                     /locus_tag="PLD99_02780"
     CDS             891..1052
                     /locus_tag="PLD99_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HRH25836.1"
                     /translation="MDEKVIGYCVKCKDKREMKDVQKVEIKPGRPAAKGTCSVCGTGM
                     YKILPKSAA"
     gene            1086..1298
                     /locus_tag="PLD99_02785"
     CDS             1086..1298
                     /locus_tag="PLD99_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25837.1"
                     /translation="MMKKVLEHKLKSLPEAQRNAIIGAVEKNPDFFKKIGDEIEKRKK
                     SGQSEIEATMAVMRENQKELQSLMMQ"
     gene            1299..1880
                     /locus_tag="PLD99_02790"
     CDS             1299..1880
                     /locus_tag="PLD99_02790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRH25838.1"
                     /translation="MKKKKIFILLGHPDKDTLNGTLASEYERGAEEAGHETRRMNLGD
                     MQFDPILHKGYKTIQELEPDLKIFQENVKWADHFVIFYPSWWSTMPALLKGLMDRVWL
                     PGFAYHFTHNGNWWQKLLKGRSATMVVTSDTMPIIQRIMFGDTTNELRKGILGFSGFS
                     PVRVYKFGYLKHFGMWQSERMKRKVNNLGKKAK"
     gene            2289..>3022
                     /locus_tag="PLD99_02795"
     CDS             2289..>3022
                     /locus_tag="PLD99_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRH25839.1"
                     /translation="MSLSIGIVGLPNVGKSTLFNALTKKSVPAENYPFCTIDPSVGIV
                     PVPDERLDRLGEMSKSKKIIPAVVEFVDIAGLVKGASEGAGLGNKFLQNIREVDAIIQ
                     VVRIFEDKDIIHVHDKVDPLFDIEVINMELEAAGIKKPVLYVVNTSEVAENKRDILDK
                     IPGPYIEVDAIFGTGLDQLIKASYDLLNLITFLTTGEKESRAWTTKRNATAPEAGKSI
                     HTDFQEKFIRAEVVAYDKLIEAGSYAA"
BASE COUNT          857 a          630 c          766 g          769 t
ORIGIN      
        1 tggcttatgt cgttagcaag gtctcggaag gtccgtcctg ccccgcaact ctggagcgag
       61 ctccagtcct ccaggacaat acgatcaatt catgtcctgt tgccatctcc cacataatca
      121 caggtgacaa gtgatgtcaa gcgtctgttg attttctcgg ccttgttggg taatattgtg
      181 ccttgtaggg cccttagctt agttggtaga gcgcctcatt tgcaatgagg aggtcatcgg
      241 ttcgaatccg atagggtcca cagtggtatc tggtcagaat tgaatgtcaa ttaaccacgg
      301 gaggttcttt tgaaacagat gtatcgcatc acatcgtgga ggtttgatgt gcgctcgacc
      361 acccactcca ctccagagtt tgagggagag actgtcgagg attgcgacag gcaggcgctt
      421 gctcatttcg atgaggtctc tgcgaagcca gataatgctt gggagtgcat ggatattgca
      481 cgcattgatg tgcccgctgt cgccgaaaag gtcacccgtc ttgcaaagaa tgagaatgcg
      541 aagcaagacg agcttcgcga ttaggtttca tcaccagcta aacaaaagct ggttttttca
      601 tgccaaataa aaccgcgaac tactttggtt cgcggttgat acggaatgca tgcccagagt
      661 tgcgaaagat ctcatcagcc ctcctgaaga gttctgccct ctctggagtg agaggcgtac
      721 ccgcccttat ctccctcaag atctcgatcg catccttgcc ccttactgtt ggggtcggtt
      781 ggattggtct cgtcactgtt ttctcctcaa gatggtggtc tatacccaga ctagcacaat
      841 ttgagcctaa aaggtggtat aataggaggg taatagaata atataaccac atggacgaaa
      901 aagtcattgg atactgtgtt aagtgtaaag ataagcgtga gatgaaggat gtacagaaag
      961 ttgagataaa gccaggtcgc ccagcagcta agggcacttg ctctgtttgc ggtactggta
     1021 tgtacaagat tctcccaaag tcagcagcct aactcctaaa gcaaatgggt aaaatacaaa
     1081 acttcatgat gaagaaggtt cttgagcata agctaaagag cctgcccgaa gcacaaagaa
     1141 atgccattat tggtgctgta gagaagaacc cggacttctt caagaagatt ggagatgaga
     1201 tagagaagag gaagaagagc ggtcagtccg agatcgaggc taccatggct gtcatgcgag
     1261 agaatcagaa ggagttacaa agcttaatga tgcaatagat gaagaagaaa aagattttta
     1321 tactacttgg tcacccagac aaggacacac taaatggcac tctagctagc gagtatgagc
     1381 gaggtgcaga ggaggctgga cacgaaacca ggaggatgaa ccttggggat atgcagttcg
     1441 atcctatact ccacaaaggg tacaagacca tacaagaact cgagccagat ctaaagatat
     1501 tccaagagaa tgttaagtgg gctgaccact tcgtcatctt ctacccatct tggtggtcta
     1561 ctatgcctgc attgcttaag ggtcttatgg atcgagtttg gttaccaggc tttgcatatc
     1621 acttcactca caacggcaat tggtggcaga agctcctcaa ggggcgctca gctactatgg
     1681 ttgtgacatc agacacaatg ccaataatcc aaagaatcat gtttggtgat actaccaacg
     1741 agcttaggaa gggtattttg ggattctctg gcttctctcc agtaagagtg tacaaatttg
     1801 gatatctcaa gcactttggt atgtggcaaa gtgagagaat gaagaggaag gtcaataatc
     1861 tcgggaaaaa agcaaaataa aatcgcagtt aactagactg cgatcggtga gccgactcat
     1921 taaggtactg ttttccacct ccaattcttg ctaggtgaaa gcagaaaatg agcagtatat
     1981 aaattgcgat aaatgagcgg tgagcttgaa tcatcactag ggctgtcacc gtcaaaattt
     2041 tgaagagtat gagatacgga tctctcaact tcttgaggta ccacatcacc caatcttttg
     2101 tcgagttgat gattcgtgat tcatagagta tctgtgcagt tcttgctcct gccttgatgg
     2161 attgatcaaa tatccgagcg attaggagga ggattagagt cagaataatg ctcgcgataa
     2221 ttatctgagg cgtgaacatt cttactcctt gtgtgtgtgc ggttcttcag ctacaatact
     2281 gactcaacat gagtctgtca attggtatcg ttggtttgcc aaatgtgggg aagtcgactc
     2341 tcttcaacgc gcttacaaag aagagcgtgc ccgcagagaa ctaccccttc tgcaccatag
     2401 acccttctgt gggtattgtg cctgtgccag atgagagatt ggataggttg ggtgagatga
     2461 gtaagtcgaa gaagatcatc cctgccgtgg ttgagttcgt agacattgca ggacttgtta
     2521 agggagcctc tgagggtgca ggacttggca acaaattcct acagaacatt cgtgaggtgg
     2581 acgcgattat acaagtagta agaatctttg aggacaaaga cattatccac gtgcatgaca
     2641 aggtggatcc acttttcgat attgaggtga tcaacatgga gctagaagct gcgggtatca
     2701 agaagcctgt gctttatgtg gtcaacacct ccgaagtcgc agagaacaag cgagatattt
     2761 tagataaaat tccaggacca tatatagagg tagacgccat ctttggcacc ggactcgacc
     2821 aacttattaa agcgagttac gaccttttga acctcattac ctttctaact acaggagaga
     2881 aggagtctcg tgcatggaca accaagagga atgcaactgc accagaagcg gggaagtcta
     2941 tacatactga ttttcaagag aagtttatcc gtgctgaggt tgtggcctac gacaaactta
     3001 tagaagcggg atcctatgcc gc
//
LOCUS       DAORIT010000032         2926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_8654_length_2926_cov_54.229188, whole genome shotgun sequence.
ACCESSION   DAORIT010000032 DAORIT010000000
VERSION     DAORIT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8654_length_2926_cov_54.229188"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1053
                     /locus_tag="PLD99_02800"
     CDS             31..1053
                     /locus_tag="PLD99_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRH25840.1"
                     /translation="MDQSNGNIVNISFSSWVKAVLVLVLAYAAYLIKDIFLVIIASIV
                     IASALEPAAHWAKSKGIPRLPTILSVYVILAILFAGLFYFLFLPLLGEVSGFISGFSG
                     YADSFSANSSLGNSLNISNLGEKLNTILLSLSQGVISSATLLFGGITSFVLIVILSFY
                     LAVQDDGVGKFLRIVTPLNREKYILDLWNRSRQKIGLWMQGQLLMSVIIAVLAYLGLL
                     LLGIPNALLLAVGAGLFEIIPLFGPILAAIPAVIIGFTTGGTSIALLVAGLFIIIQQF
                     ENQLIYPLVVKKVIGVPPMVSILALLIGAKLAGFLGMLISVPLAAIFIELISDMEKAK
                     IAQGNK"
     gene            1076..2494
                     /gene="metG"
                     /locus_tag="PLD99_02805"
     CDS             1076..2494
                     /gene="metG"
                     /locus_tag="PLD99_02805"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRH25841.1"
                     /translation="MSRNKAQYITTTLPYVNSKPHMGHALEFIRADAIARWKKKLGNE
                     VYFNTGTDEHGQKIFTAAGSANMGVQEYVDKFALEFKALLPLLNISDDIHFVRTTDPK
                     HMQAAQEFWKRVNDNGFIYKKSYKTKYCVGCESEKTDSEIVDGKCAIHTNLELEVIEE
                     ENYFFKFSAFGDRLLELYKDDFIIPDSRLNETKEFVKRGLEDFSISRLREKMQWGVPV
                     PGDDKHVMYVWFDALVNYISTLGWPEDTDAFNKFWVEGEPIQFCGKDNTRFQGAMWQA
                     MLIAAGLPNSKKIVVNGFVTGEGGVKMSKSLGNVVDPVELSHKFGAEALRYFVCREIS
                     TFEDSPFTVERFRESYNANLANGLGNLVSRTMKLAETYLDGPVVVEHKFHNTIKDLLE
                     KFEIQKAMNHIWGEIGELDQFIQEKKPWETKDKKVIAEMVTKLSHIGHSLMPFMPETS
                     KKIVEAISNNKIETGLFPRIDA"
     gene            2487..>2926
                     /locus_tag="PLD99_02810"
     CDS             2487..>2926
                     /locus_tag="PLD99_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRH25842.1"
                     /translation="MPKYFDIHSHLNFKDFEGDLDAVIQRLRDTDTHTIVVGTDYESS
                     KWAVELAEKYEEIYACIGVHPVDNPTRVFDDEGFKELIKNKKVVAVGECGFDFFHAKK
                     EEDYERQEKLFDTQIEFALEYDKPLMIHSRGAYQELLEKLEGLK"
BASE COUNT          793 a          587 c          689 g          857 t
ORIGIN      
        1 tgaagaaact cttggtgtta tgattgtccc atggatcaat ctaatggcaa tatagtcaat
       61 atttccttct ctagctgggt taaggcagtg cttgtacttg tcttggctta tgccgcatac
      121 ctaattaagg acatcttcct cgttattatt gcctctattg ttatagcctc agcccttgag
      181 cctgctgctc attgggccaa gtcgaagggt atacctagac tccctactat tctctcggtc
      241 tatgttatct tggcaatact cttcgcaggt ctcttctact tcttgttctt gccactcttg
      301 ggtgaggtat cgggctttat ctcgggcttc tctggctacg cggactcatt ctctgcaaac
      361 tccagtcttg gtaactcttt aaatattagt aaccttggag agaagttgaa taccatcctc
      421 ctctcgctct cgcagggtgt gatctcctcg gccacacttc tcttcggagg tataactagt
      481 tttgtgctta ttgttatcct ctctttctac ctcgcggtgc aagatgatgg agttggtaag
      541 ttcttgcgca tcgtaactcc tctaaatagg gagaaatata ttcttgatct atggaatcgt
      601 tcgagacaaa agattggtct ctggatgcag ggacaactct tgatgtccgt catcattgcc
      661 gtacttgcgt acctaggtct attgcttctt gggattccga acgccttgct cttggctgtt
      721 ggtgcaggac tcttcgagat cattccactc ttcggtccaa tacttgcagc tatccctgcg
      781 gttatcattg gctttaccac aggtggcaca agcattgctt tactcgtggc tggactcttc
      841 attatcatcc agcagtttga gaaccaactt atctacccac tggtggtaaa gaaggttata
      901 ggcgtgccac caatggtatc gattcttgct cttcttatag gtgccaagct tgcaggattt
      961 ctaggtatgc ttatctctgt tcctcttgct gctatattta ttgagcttat ctcagatatg
     1021 gaaaaggcta agatcgctca aggtaacaaa taagttgcca aatagtttta aaccaatgag
     1081 tagaaacaaa gctcaatata ttacgaccac tctgccgtat gttaactcta agcctcatat
     1141 gggacatgcc ctggaattta ttcgtgcaga cgcaatcgcc aggtggaaaa aaaaattagg
     1201 taacgaagtt tactttaaca ccggtaccga cgagcatggt caaaagattt ttaccgcagc
     1261 agggtccgct aatatgggcg tacaagagta tgtcgacaaa tttgctttgg agtttaaggc
     1321 acttctaccg cttttaaata ttagtgacga tattcatttt gtccgtacaa cagatccaaa
     1381 gcacatgcag gctgcacaag agttctggaa gagggtgaat gacaacgggt ttatatataa
     1441 gaagagttat aagaccaagt actgtgttgg ttgcgagtcg gagaagacgg actcagagat
     1501 agtagatggc aagtgtgcta tacataccaa cctggagctc gaggttatag aagaggagaa
     1561 ttacttcttc aaattcagtg ctttcggcga tagacttctt gagctataca aagacgattt
     1621 tattattcca gattctagat tgaacgaaac caaagagttt gtgaagcggg gtcttgagga
     1681 cttctctata tctagactgc gggagaagat gcagtggggt gtaccagtac cgggggatga
     1741 taagcatgtt atgtatgtgt ggttcgacgc cttggttaat tacatctcca cccttggctg
     1801 gccagaagac actgatgctt tcaataagtt ttgggttgag ggagagccaa tacagttctg
     1861 tggtaaggac aacaccaggt tccaaggagc tatgtggcag gcgatgctta tagcagcagg
     1921 tctaccaaac agtaagaaga tagtggttaa tggcttcgtt acaggcgaag gtggggtaaa
     1981 gatgagtaag tcactcggca atgttgttga cccagtagag ctttcacata agtttggtgc
     2041 tgaagctctc cgctacttcg tctgccgaga gatctcaacc ttcgaggaca gcccctttac
     2101 cgttgagagg tttagagagt cttataatgc taaccttgcg aatggcctcg gtaatcttgt
     2161 gtctcgtacc atgaagcttg ctgaaaccta tttggatggc ccggtagttg tagagcacaa
     2221 gttccacaac acgatcaaag atcttttaga aaagtttgag atccagaagg cgatgaatca
     2281 tatttggggg gagattggtg agcttgacca atttatccag gagaagaagc cctgggagac
     2341 aaaggataag aaggttattg cagagatggt cacaaagctc tcgcatattg gtcactcact
     2401 tatgcctttc atgccagaaa cttctaagaa gattgttgag gcaatttcta ataacaagat
     2461 tgagactggt ttattccctc gtatagatgc ctaagtattt cgacattcac tcgcatctta
     2521 acttcaagga ttttgaagga gatcttgatg ctgtaataca aagattaaga gacactgata
     2581 cacacaccat agtcgtcggt actgattatg aatcttctaa atgggcagta gagttggcgg
     2641 agaagtatga agaaatatat gcatgtattg gcgtgcaccc agtggataat ccgactaggg
     2701 tattcgatga cgagggattt aaagaattaa ttaagaacaa aaaagtggtt gctgtgggtg
     2761 agtgtgggtt cgattttttc catgccaaga aggaggaaga ttatgagagg caagaaaaac
     2821 tttttgatac acaaatcgag ttcgcactcg aatacgacaa acctctgatg atccactcgc
     2881 gcggtgccta ccaagaactt ttagaaaagt tagaaggtct aaaaaa
//
LOCUS       DAORIT010000033         1827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Parcubacteria group bacterium isolate AMR_MDS_5570
            NODE_20514_length_1827_cov_59.697517, whole genome shotgun
            sequence.
ACCESSION   DAORIT010000033 DAORIT010000000
VERSION     DAORIT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867787
            Sequence Read Archive: SRR1145916
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Parcubacteria group bacterium (wastewater metagenome)
  ORGANISM  Parcubacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 05/03/2023 17:30:48
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline (PGAP)
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 564
            CDSs (total)                 :: 519
            Genes (coding)               :: 519
            CDSs (with protein)          :: 519
            Genes (RNA)                  :: 45
            rRNAs                        :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs               :: 1, 1 (5S, 16S)
            partial rRNAs                :: 1 (23S)
            tRNAs                        :: 40
            ncRNAs                       :: 2
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Parcubacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20514_length_1827_cov_59.697517"
                     /isolate="AMR_MDS_5570"
                     /isolation_source="Conventional activated sludge (CAS)"
                     /db_xref="taxon:2026774"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="31.3 N 121.5 E"
                     /collection_date="2013-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1451
                     /gene="ftsZ"
                     /locus_tag="PLD99_02815"
     CDS             249..1451
                     /gene="ftsZ"
                     /locus_tag="PLD99_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRH25843.1"
                     /translation="MPQVKPEIESFARIKVIGVGGSGKNAVNHMINSKVRGVDFVAVN
                     TDAQDLHNSLAKKKVHIGKNLTRGLGAGMNPELGRKAVEETKEEIQEVIKGADMVFIA
                     GGMGGGTGTGAAPVIARTAKENGILTVAVVTKPFYFEGVQRMRIAEQGIEELKQVVDA
                     IIIIPNDRLLSTIDKDTSARNAFAICDNILKEAVEGISDLITMPGIINIDFADIRAVM
                     QDAGAALMGIGLGQGDKRAEDAARAAISSPLLEISINGAKGVLFSIAGGDDLTMFEIQ
                     DAAKVITESVDPNARIIFGTVHDDKLKKGEVKITVIASSFPENVQKKVTSLFQNREVM
                     EDEDKGKNKIFGSFTSSPKPEKSPEPVIIPTPAPAAPAKKVEPVVVNDDEDDWGAVPS
                     FLRRSKLK"
     gene            complement(1484..1786)
                     /locus_tag="PLD99_02820"
     CDS             complement(1484..1786)
                     /locus_tag="PLD99_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRH25844.1"
                     /translation="MSFGYDKWRLIEEVLLSDSDVGPTEDETRAFLIAFATAVGYISP
                     LATDMESVRRREEALRKVRAEVAPLIEASRRAERMTQDDHRILIDLHPSFGIGGGR"
BASE COUNT          514 a          457 c          413 g          443 t
ORIGIN      
        1 ttggtgggga ttttaatttc caaaatataa ttttctgaat aaaacattct gaataagtaa
       61 gtcaaacaca ttttcctggc tcaaacacaa tatatttcag cctcaattca acatatttga
      121 agactattct tttcgaagaa taatccaacc tgttttatgc gagtgtcaac tgtttatttt
      181 tccccaatgc tcatgtacta tgtttgcacc tcaaatctta gaggcacaat tctcacaaaa
      241 acatacttat gccacaagtc aaaccagaga tagaatcatt tgcacgtatc aaggtcatcg
      301 gtgttggtgg ttccggtaag aacgcagtga accacatgat caactcgaaa gttcgtggag
      361 ttgatttcgt cgcagtgaac actgatgcac aagatctgca caactctctc gctaagaaaa
      421 aggtgcatat cggcaagaac ctcactcgag gtcttggtgc tggtatgaac ccagaactcg
      481 gtcgcaaggc agtagaagaa acaaaggaag aaatacaaga agtcatcaaa ggtgcagaca
      541 tggtcttcat cgctggtggt atgggaggag gtaccggcac aggtgcagcc cctgtcatcg
      601 ctcgtaccgc taaggagaat ggcatactca ccgtcgctgt cgttaccaag ccattctact
      661 tcgagggtgt tcagcgcatg cgtattgccg agcaaggtat cgaggagttg aagcaggtgg
      721 tagacgccat catcatcatc cccaacgacc gactcctctc tacaatagac aaggacacta
      781 gtgccaggaa cgccttcgcc atctgcgaca acatacttaa ggaggcggta gagggtatct
      841 ccgacctcat cactatgcca ggtattatca acatcgactt cgccgacatc agagcagtca
      901 tgcaagacgc tggtgccgct ctcatgggta taggactagg tcagggagac aagcgtgccg
      961 aggatgccgc tcgtgccgct atctcctctc cacttcttga gatctctatt aatggtgcta
     1021 agggtgtcct cttctctatc gctggaggcg acgatcttac aatgttcgag atccaagacg
     1081 cggccaaggt tattactgag tcagttgacc ctaacgcccg cattatcttc ggtacagtac
     1141 atgacgacaa gttgaagaag ggtgaggtca agatcacagt tatcgcctca agcttccctg
     1201 agaatgtaca gaagaaggtt acctccctct tccaaaacag agaggtcatg gaggacgaag
     1261 acaagggcaa gaacaagata tttggtagct ttacctcatc tcctaagcca gagaaatcac
     1321 ctgagccagt aatcatccca acacccgctc cagctgctcc agcaaagaag gttgagccag
     1381 tggttgtgaa cgacgacgaa gatgattggg gtgcagtccc ctcattcctt aggagatcaa
     1441 aactaaaata aagaaaaaac cagtcccata cggggctggt ttgctatctg cctccaccaa
     1501 taccgaatga cggatgaagg tcaatcagaa ttcgatgatc gtcttgtgtc atcctctctg
     1561 cccttcttga ggcttcgata agtggagcca cctctgctcg tactttgcga agggcttcct
     1621 ctcgccgtct cactgactcc atgtcagttg cgaggggtga gatataaccc acggcagtag
     1681 cgaaagcaat gagaaaggct ctcgtctcat cctcagtggg accgacatca gaatcactca
     1741 aaagtacctc ctcgataagt ctccatttat catacccaaa actcataact tatccacggt
     1801 tttacgacaa gccctcccct tgtcggg
//
